LOCUS       DATRQA010000001        49641 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.001, whole genome shotgun sequence.
ACCESSION   DATRQA010000001 DATRQA010000000
VERSION     DATRQA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49641
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.001"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(139..1206)
                     /locus_tag="VBD27_000001"
     CDS             complement(139..1206)
                     /locus_tag="VBD27_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7024049.1"
                     /translation="MAYYRKRDNGWEYRISYKDESGKFRQKSKSGFKTKKLAQAAARD
                     IEKKLSQNILTDGEVTLYDFVKTWSEVYKRPYVKDKTWETYTKNFRHIKTYFKDIKVK
                     DITPLYYQKRLNEFGEKYAQETLEKFHYQIKGAMKVAVREQVIHFNFADDAKVKSQIE
                     SRAEENDFLEESEYKALLSLTRENIQYVSYFTLYLLSVTGLRFSEVMGLTWSDIDFKN
                     GILDINKSFDYSNTQDFADLKNESSKRKVPIDSNTIDILREYKKNHWQANIKNRVCFG
                     VSNSACNKLIKKIVGRKVRNHSLRHTYASFLILNGVDIVTISKLLGHESPDITLKVYT
                     HQMEALAERNFEKIKNIFLVA"
     gene            complement(1330..1734)
                     /locus_tag="VBD27_000002"
     CDS             complement(1330..1734)
                     /locus_tag="VBD27_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HEO7024050.1"
                     /translation="MALFGGKDKEIEVELFTSEPNERVFEFKKSKTIVRIDDYFIRIA
                     RKSNMSNMLLHGLDGEKSILLSEITAYQLKEPSSTVGYLQLVYPGSSDTKGGVFDAVK
                     DENTVTFSKDEKIAILELKNAIENALKNKVKK"
     gene            complement(1756..2502)
                     /locus_tag="VBD27_000003"
     CDS             complement(1756..2502)
                     /locus_tag="VBD27_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO7024051.1"
                     /translation="MKYTPLSREQYFDYHNKAYQIISSIDKMKENVSYQDVIKHFEQN
                     YPILFNFLDYDEMKERYPELPDYHPTDKDIKYRGLVSNRTVTYTDRVLCESCAGLTVP
                     DLDLGRYIIYINQHTNTKGRVIFTILHELSHIHCHLNQTENQPIYMSLMSKNASEKYP
                     KELIPIEKEADTVASILYLTDERLKKALTTRESFEDIQRETDISKPALHNRLMNYLIY
                     NLQYAESYALKLVLNYRQGGGQIFNILRLQ"
     gene            complement(2511..2891)
                     /locus_tag="VBD27_000004"
     CDS             complement(2511..2891)
                     /locus_tag="VBD27_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7024052.1"
                     /translation="MFFTFEKIKELADKQGISLNQLEEQLDFPRNTIYNMKKSTPNVE
                     RVSKIADYFNVSTDYLLGRTENPKIADGQDNTAVDDLTQQAIVLFRKETEGLSASEKE
                     RFNSALEGLMKTARQLIQDDSNWK"
     gene            3179..3343
                     /locus_tag="VBD27_000005"
     CDS             3179..3343
                     /locus_tag="VBD27_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024053.1"
                     /translation="MGIGSMTVTVKVTNMDKFIELSKEFNKKARELEELAHELKSFDF
                     EGEVVSTDSN"
     gene            complement(3326..3796)
                     /locus_tag="VBD27_000006"
     CDS             complement(3326..3796)
                     /locus_tag="VBD27_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024054.1"
                     /translation="MNLNQIRIIEACHKFLIGITNFEEELQDDVLVYRYKGNLVSFET
                     YQEYEQRSFVDYNLKYGYLDDTRTYIDDRADLIAAFPSEEHLRALQRVNDAEQARVQI
                     FKLLSQVNLDSLSEKNSHIKKDNFGYDFFNFATKEEYPVYLFSDDESFELVAIS"
     gene            3857..4069
                     /locus_tag="VBD27_000007"
     CDS             3857..4069
                     /locus_tag="VBD27_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7024055.1"
                     /translation="MSQQQKKWIQLVKDKLNEEQMTQTHLARACGVAKATISELLKYG
                     KGSVRLKNKVSDILHIDESWTDLEED"
     gene            4069..4410
                     /locus_tag="VBD27_000008"
     CDS             4069..4410
                     /locus_tag="VBD27_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024056.1"
                     /translation="MPITREMTITEINVLNAIKNSATYDLPIQARELRQQLGLSKRSL
                     EAVIENLRVIYKQPIVAKKKQPSGYYLPRNEEERNDGLAPYKRQILTEQNNLAAIMSV
                     NLEEYWATEKA"
     gene            4457..4987
                     /locus_tag="VBD27_000009"
     CDS             4457..4987
                     /locus_tag="VBD27_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO7024057.1"
                     /translation="MNEVWKDIPIVKGYYQVSNLGRVRSIGRTVNAKQRTRKTKGRIL
                     KQSLSSGYAIVTLSVNGLRKSIRVHRLVAEAFIPNPINKRTINHIDENKLNNRVDNLE
                     WATDKENANHGNRTTKSSLGRCKPVEQFTLEGEFINTFDSIKSASMKTGISSQRITAT
                     AMGHQKQTHGYKWRYA"
     gene            4987..5271
                     /locus_tag="VBD27_000010"
     CDS             4987..5271
                     /locus_tag="VBD27_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO7024058.1"
                     /translation="MNDLMIQMLDQFEAGLMDRTMKVMHVVTDEKYRFPLELNKSQCA
                     LMLLGTKDTGTFDERFNCHKDFPRIPNAREKYPRDEVIEWYHKNWQRTVI"
     gene            5295..5513
                     /locus_tag="VBD27_000011"
     CDS             5295..5513
                     /locus_tag="VBD27_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024059.1"
                     /translation="MNNLQIIAVGTLISVTLIESLISNIKLRKALKNKQTAGTVIAQY
                     IPQRGFIDYKTGRRVDIDPETKKEVFVD"
     gene            5522..6853
                     /gene="dnaB"
                     /locus_tag="VBD27_000012"
     CDS             5522..6853
                     /gene="dnaB"
                     /locus_tag="VBD27_000012"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO7024060.1"
                     /translation="MDELKVLPHDIQAEQSVLGSIFIKPEKMIEVAEYLKPNDFYRPA
                     HKILFKAMVSLADRGEAIDIVTIKSTLESTDELGMVGGISYIAEIVNAVPTSSHAEHY
                     AKIVAKKAQLRSIIDNLSDSIGNAYDEDMDIDEIIAKAERSLIEVSQASNKSSFRPIH
                     DVLLENHSKIEERSNNTSQITGIETGFYDFDKLITGLHEDQLIVLAARPAMGKTALAL
                     NIAQNVATKSNKAVAVFSLEMGAESLVERMLSAEGTIINHHIRTGNLTVNEWQRLIYA
                     QGQLAEAPIFIDDTAGVKITDIRARARRLSQETDGLGLIVIDYLQLIQGSRSDNRQQE
                     VSEISRQLKIIAKELKVPVIALSQLSRGVEQRNDKRPIMSDLRESGSIEQDADIVAFL
                     YRDAYYQDKKEGQPENDITELIIRKNRHGNLGTVKLYFHKEYTKFSSVEEE"
     gene            6855..7457
                     /locus_tag="VBD27_000013"
     CDS             6855..7457
                     /locus_tag="VBD27_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024061.1"
                     /translation="MKKFDHYFSNHIAVKNESDTVTIVDYYNPEKGYEYNLRYIFDRK
                     NSSLAITGDFGELVARNFYNMGEWDTFYNHYTGHLGYFLEKLLCSSRPVHYYDTDKAK
                     REILNEFFDGADYDDLDFDDEFLLDQLFDNFDEDKGFTYILPEAFERFSEDMEEYEVY
                     EFLSRAGRYVNDVFELYLDAYKRAYEYLTKEVDDGTTQNV"
     gene            7435..8181
                     /locus_tag="VBD27_000014"
     CDS             7435..8181
                     /locus_tag="VBD27_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HEO7024062.1"
                     /translation="MAQRRMFSRKITETDRFLEMPLSSQALYFHLNMGADDEGFIDKA
                     KTIQRTIGASDDDMKLLIAKGFLIPFDSGVVVIRHWRIHNYIQSDRFQSTLYQSEKAQ
                     LEYDKSKTSSLKPIGNCIQNVSKMETQVRLSKGSLDKDSLTTYPTVSDNEEEDIPYKE
                     IISYLNEKANRNYRPNIQKNKTLIKARWSEGFRLDDFKHVIDTTVKDWSGTKYEKYLR
                     PETLFGSKFEGYLNQAPRIKTETIDGRLGF"
     gene            8182..9003
                     /locus_tag="VBD27_000015"
     CDS             8182..9003
                     /locus_tag="VBD27_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7024063.1"
                     /translation="MNPFKNFETRQVLEETCEVHGCQLWLTKVPIKGRLEELKQCPEC
                     TKAAINIFENKLNSQSKINSKLADTYAVFERDSLVSDKLRGKSLENYEIKDDIDQQAI
                     NYAKRMEQFYRQDRTGNAIVTGPSGVGKSHLTYGLAKFMNEQFKAYESPKSVLFISLV
                     SLFTKIRESFKADNGYRQAEMIELLTSVDYLFLDDLGKESRKGDSQNNEWTHQILYEI
                     LDNRSNTIINTNLSSKEIKALYADNYGNGALSSRILEGVTGNSFAYPKDMEDRRY"
     gene            9003..9251
                     /locus_tag="VBD27_000016"
     CDS             9003..9251
                     /locus_tag="VBD27_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024064.1"
                     /translation="MSYKATFMLYGYKAVPIGLFDTKELAAQAIVDHIIENSGIPRPR
                     VVATMKIRSKEIRIDYGSKTCYYLITLINNKTEEKQHD"
     gene            9244..9738
                     /locus_tag="VBD27_000017"
     CDS             9244..9738
                     /locus_tag="VBD27_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO7024065.1"
                     /translation="MTKTMTLEEKVEQWFIDRNLHEANPVKQFQKMMEETGELYSGIA
                     KDKSEIIYDSLGDMQVVLIGIEQQIKNGAQIEASPQDMELLLLASSLGEMAQKLYKHI
                     FHNETKTPLIRPELSLLHSTIHAIAIHNLTTADDCLSIAYEEIKDRKGKLINGIWVKE
                     EDLK"
     gene            9735..10064
                     /locus_tag="VBD27_000018"
     CDS             9735..10064
                     /locus_tag="VBD27_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEO7024066.1"
                     /translation="MKNMNWKAFFNNLKAFASVIIITVFVLGFNYLFYEIGYQKGHQE
                     ADRVIIYVADNAGAEMFGKITDKEIIEGRHTVTAGAYGKFLVTEEQYNEITVGDDIPD
                     YLKGRGN"
     gene            10064..10483
                     /locus_tag="VBD27_000019"
     CDS             10064..10483
                     /locus_tag="VBD27_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEO7024067.1"
                     /translation="MTKCNFEAGHKPIDTIGQLAKEVLDGKYGDGEERRRNLGCLYDL
                     VQTRVNYMVHERDSKFTGIKIYDNVNKPSHYQGKNGMEAIDVVRNFIGQLTGVSAFYW
                     GNSMKYLLRFQGKNGLEDLKKARKNLDWLIEEMEKEK"
     gene            10480..10686
                     /locus_tag="VBD27_000020"
     CDS             10480..10686
                     /locus_tag="VBD27_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024068.1"
                     /translation="MKKSIGILEFKEFDAFVGGKVMPVYLHSANPNVPTTNKNEAMRV
                     TKKRYRKEFSDKYIFRKVGEVDDQ"
     gene            10676..11092
                     /gene="ssb"
                     /locus_tag="VBD27_000021"
     CDS             10676..11092
                     /gene="ssb"
                     /locus_tag="VBD27_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7024069.1"
                     /translation="MINNVVLIGRLTRDVELRYTPSNIANATFNLAVNRNFKNAAGDR
                     EADFINCVMWRQQAENLANWTKKGMLIGITGRIQTRSYENQQGQRIYVTEVVADSFQI
                     LEKRDNSTNQASMDDQLPPSFGNSQPMDISDDDLPF"
     gene            11102..11362
                     /locus_tag="VBD27_000022"
     CDS             11102..11362
                     /locus_tag="VBD27_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024070.1"
                     /translation="MNEYKKPTYIVIQEAMAERIRFLEDELYNRAYKDIERLEVENDR
                     LKVRCLDLQLENADYVWDDMCRSAIARAKSENRRPHRRWRGR"
     gene            11359..11706
                     /locus_tag="VBD27_000023"
     CDS             11359..11706
                     /locus_tag="VBD27_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7024071.1"
                     /translation="MKLRLEELRNKLGLSRRDVHEATGISMNTLLHYEKGGTPSIGQV
                     ENIAKAYNINPAWLLGWIDEGQQPQPLETVVEKIIYVEKEGCRLPPYWNNDNNGRIIK
                     WKESRRAFQRRTI"
     gene            11703..12167
                     /locus_tag="VBD27_000024"
     CDS             11703..12167
                     /locus_tag="VBD27_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024072.1"
                     /translation="MTKKTASKTRRDFLEFELEAKYLKIDKLIGQRRHELERIYAVKN
                     LTMPGIDDSGASRSGTSTNTSENLAVAYASDPMILKLEEFQNAISELLEVLEPDDKKI
                     FHLRWGEHTGYDWIQVWHIMENGETGYLYRHSKQIYRRREVILDTLAKLLFM"
     gene            12277..12819
                     /locus_tag="VBD27_000025"
     CDS             12277..12819
                     /locus_tag="VBD27_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7024073.1"
                     /translation="MNIVEPLRDKDDIQAMKDYLSSWNEKYYMLFLLGINTGFRVGDI
                     LKLKVKDVQGWHIKVREQKTGKYKSIKMTRPLKNELREFVKDKELHEYLFQSRVGKNK
                     ALSYKTVYWFLKRAAEDLGIDNVGTHTMRKTFGYHYYKKYKNVADLMSLFNHSSPAVT
                     LIYICVRQDELDTKMSNFSL"
     gene            13109..13396
                     /locus_tag="VBD27_000026"
     CDS             13109..13396
                     /locus_tag="VBD27_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024074.1"
                     /translation="MKDENAFDGIRLEAGSTLEIYLTKNDLEHIANGYEVTLDIKPNE
                     TVNKIVIKPSFVNNILNPLINYDKRIVSEADLKFRDISREVARDSFALGSM"
     gene            13396..13755
                     /locus_tag="VBD27_000027"
     CDS             13396..13755
                     /locus_tag="VBD27_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO7024075.1"
                     /translation="MGYRNPKHSDWFRAWQIKFYNSKPWRTLRNKIRKAKRMRCDMCG
                     RLIHGKSIVDHIIEIDETNYQDESITLNEENCQLLCLECHNTKTFQSKINLNLDNRNI
                     NLFLIFLFLNFCGLPPF"
     gene            13843..14328
                     /locus_tag="VBD27_000028"
     CDS             13843..14328
                     /locus_tag="VBD27_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024076.1"
                     /translation="MILKINEVLEKLGISRATLTRYRKKLGIFEETRSNITKSQFKEL
                     EKLANQRQKYTRQERVELSRKTFKLIPKEKMLEISDNDSVVLKNLKTQYNHNQKVIEN
                     FQLEINKVINDGELPDKYLLDGMEKYQKLNMQIMSTIEKQSPQGDSLKEMIQEKLARY
                     G"
     gene            14321..16033
                     /locus_tag="VBD27_000029"
     CDS             14321..16033
                     /locus_tag="VBD27_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEO7024077.1"
                     /translation="MVEMRYFDKYAQLIYTGKIRICKLTMKSIRRVERYKEQYLFKQE
                     EADKRIEFIEEECSNTKGLAGKLRLALPQKVWLETTWGFYHTVEVTKTNPDTLEEYTD
                     YEERRLIHEVPIIVPRGTGKTTLGSAIAEVGQIIDGEWGADIQLLAYSREQAGYLFNA
                     SRAMLSNEESLLHYMREADILRSTKQGILYETTNSLMSIKTSDYESLDGTNAHYNIFD
                     EVHTYDDDFIKVVNDGSSRKRKNWITWYISTNGTKRDKLFDKYYNIWVDILDDKIIND
                     SVMPWIYQLDDVSEIHDPDMWQKAMPLLGITTEKETIARDIEMSKNDPAQQAELMAKT
                     FNLPVNNYLAYFSNEECKGWSDKFDESLFVGDDERNARCVIGIDLSDVNDICSISFMV
                     VRGEERHYLNKKFMPRHTIETLPKELRDKYTEWELSGMLHVHELDYNDQAYIFEELRQ
                     FMSDNRILPVAVGYDRYNARELIRLFNDYYGDICHDIPQTVKSLSNPLKVYKEKAKMG
                     KIIFDDPVATWNHANVRVKIDANNNIFPNKEKAKEKIDVFASQLDAFICYENFKEDLS
                     YYFD"
     gene            16042..17184
                     /locus_tag="VBD27_000030"
     CDS             16042..17184
                     /locus_tag="VBD27_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO7024078.1"
                     /translation="MNNYLKNLKEVFARIFRPNNRKSTRTYLQRSISYWRRNSIYLDN
                     IYNKISTDTAQLRFKHVKITRNPGGVDSMVWYEHSDLAEVLTVSPNPLEVPVVFWSNV
                     TRAMLRDGVAVVVPRWKNGRLVEIWLAKKTVTWTAESVELMLDDVAVELPLTDVWVFE
                     NPKLNVTAQLNQITELIDINLNALTEKLSDGNSSLRGFLKLPTKAADEHLKQQARDRV
                     DSMLDLAKNGGIAYLEQGEEFQELSKDYSTASKEELEFLKSQLYNAHGINEKLFTCDY
                     TEEQYRAYYSSVMKLYQRVYSEEINRKYFTKTARTQGNKLLVFFDMADMISFKDLVEG
                     GFKSKYAGLMNSNEFRETYLGLPGYEGGEVFETNLNAVRIGAEESE"
     gene            17234..17776
                     /locus_tag="VBD27_000031"
     CDS             17234..17776
                     /locus_tag="VBD27_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEO7024079.1"
                     /translation="MEKLKTFVVKSVEDESADFHFEAYASTYDNTDREGDVMAKGCFD
                     NTLKSKAVVPMCLNHDRNCVIGKHELSVDEKGLRTRSTFNLSDPEAKKTYDLMKMGAL
                     DSLSIGFFIKDYEPIDAKQPYGGWIFKEVEIFEISVVTVPANPQATVDNIKEFDMSAV
                     DKRIAQVNMRQEIMSKLAKI"
     gene            17787..19049
                     /locus_tag="VBD27_000032"
     CDS             17787..19049
                     /locus_tag="VBD27_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEO7024080.1"
                     /translation="MKKSLIELLEARQKATDELAEVKLKKATIEAKMKSSTIEDDDLE
                     QLKTDAESLVSQATAIKETIAGLDSDIEETEEELSKAAKIIKEKQKGNTPMDYLKTKA
                     AALDFVRILMDNEGSANSARKAWEANLVEKGVTNLTKILPEPVLIAIQDAFTNYNGIL
                     NHVSKDPRYAVRVALQTQVSQAKGHKAGKTKKDEDFTFLDFTINSATVYIKYAFEYSD
                     LKKDTTGAYFNYVMKELAQGFIRTIERAVVIGDGKSNSAEDKITEIKSIAEETEANLF
                     ETQEINVAGVFDNAVLETLVAGIDKMVPNTTPILVTSKAIARKLKLVKDAEGRYIDPQ
                     PFAPIATNGNVIAGFQVYIYDWMDGATNPIIAFADQAYKMIGDEAAADRFDDYDVTVN
                     RRHIELASVMGGRLAQYKSAVKFTNQAG"
     gene            19070..19405
                     /locus_tag="VBD27_000033"
     CDS             19070..19405
                     /locus_tag="VBD27_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024081.1"
                     /translation="MTILKTIKEMVEVDVEEDIFDVQLLRYINSGISYLQRNAIPVIR
                     IDKETELEGWTDIKEEDRETVLDWLHLRCVQRFDKSLMTGSAATMEWIDGEMTNLLYQ
                     LKAIYEVKT"
     gene            19402..19707
                     /locus_tag="VBD27_000034"
     CDS             19402..19707
                     /locus_tag="VBD27_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HEO7024082.1"
                     /translation="MKSSRVAIILCYDERVEVEKGVWEKKTVEKKVKAKKEKIYQRRL
                     DKAMADGQVLTARFSVRSNYVTDTLDYVKYNGKEYKVNIGTESDDGHYTIIELGELK"
     gene            19707..20054
                     /locus_tag="VBD27_000035"
     CDS             19707..20054
                     /locus_tag="VBD27_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024083.1"
                     /translation="MAKKFFTRQDIQSILETNPLKAKVFYMEREEKSSPDNVILYYRL
                     TPGSSITADDRVHMRKVTLQVSHYHKKKLDSIENLMLSNFMCEPNQLNLKQPDTDYLL
                     TTYRLEVFTNGRW"
     gene            20041..20385
                     /locus_tag="VBD27_000036"
     CDS             20041..20385
                     /locus_tag="VBD27_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO7024084.1"
                     /translation="MGGGSVKMTPLKVDIKNQVLENIKKAAQSTENDIRTKSPRRNGV
                     YEKGWTHDIIDDAAVVHNNGKEKSLSHLLENGHATKNGGFVAPREHIRPAYIKNKEKF
                     LNDMKSIKITPK"
     gene            20400..21083
                     /locus_tag="VBD27_000037"
     CDS             20400..21083
                     /locus_tag="VBD27_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7024085.1"
                     /translation="MTYQYDTREVTHGNANGFYAKIAKTDAGALDLQKPYPFTGLRST
                     SFETSQESNAYYADNVEHVRLQGKKSTEGSITTYQIPKQFMIDHLGKKLTNSTPPALI
                     DTGVNTNFIWGYAETVTDEFGAEIEEFHIWTNVKASAPKGSTSTDETSATPKEIEIPC
                     TASPNNFIVDSEKKPVSEIVWRDDSKGTVRGKFDKLFADKSPGKLIDFINEALGTTTT
                     PTTTVSGGS"
     gene            21083..21559
                     /locus_tag="VBD27_000038"
     CDS             21083..21559
                     /locus_tag="VBD27_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024086.1"
                     /translation="MIKKELSFTAFDSYGEEREHTETVRFLYSLPAIKMYEQRTGRNF
                     FDDNQKALTAYTQLALATGVNGNLSDLTDEEKIKMMPLLMEPDFMNFLTEVIPCLYGE
                     VENGRLVQNELTAETASLAPWFGDLIDIGFFSDLFYEFNRSRAKVPQDKKKPFQKL"
     gene            21745..24480
                     /locus_tag="VBD27_000039"
     CDS             21745..24480
                     /locus_tag="VBD27_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO7024087.1"
                     /translation="MTETFEGLYVKFGANTVEFDRSVKGINTALSSLKKDFNNINRQL
                     KMDPDNVDLLNRKLVNLQEQARVGAMKIAELKKQQKALGESEVGSAQWNKLQLEIAKV
                     ESQMKIVDKAMESTKKHIEDVGDPKSILNLNKELDNVAKELDIVNQKLELDTDNVELA
                     EQKMKLLGKQSELVGDKVQELKKKQAALGDEKIGTEEWRQLQNEIGQAEVEVLKIDRA
                     MDILGESSRSATGDIKEATSYLRADVMMDVADKAGQIGQKMVDAGKMTVDAWSEIDEA
                     LDTVTTKTGLTGDALAELQEIAKDIATGMPTSFQNAGDAVGELNTQFGLTGEKLKSAS
                     ELLIKYAEINETDISSSAISAKQAIEAYGLTAEDLGMVLDNVTKAAQDTGQSVDTIVQ
                     KAIDGAPQIKGLGLSFEEGAALIGKFEKSGVDSSAALSSLSKAAVIYAKDGKTLTDGL
                     NETVSAIQNSTSETEALSIASEIFGSKAAPRMVDAIQRGAFSFDDLAEAAKSSSGTVS
                     TTFDETLDPIDKLTQYSNQAKEGMAELGGKLLETVIPALEPLMGMLESSVNWFTSLNE
                     TDQQTIVILGLVTTAVMMLLGAIAPLVIAIGAIGAPVGIVVAAIVGAIAVITLIIQAI
                     MNWGAITEWLQSTWDSCAAWLSELWTNIVTTATTAWSNFTAWLSGLWSSVVSTGQSLW
                     SSFTSSLSNIFSSLITGAQSLWSSFTSTLSNLWSGLVSTGSNLFNNLSSTISGIFNGI
                     LSTASNIWNSIKSTISNAIDGAKNAVSNGVNAIKNLFNFQIKWPHIPLPHFRVSGSAN
                     PLDWLKGGLPSIGIDWYAKGGIMTKPTLFGMNGNRAMVGGEAGAEAILPLNKSTLGAI
                     GQSIANTMNTSNNINVNFSGVTIREEADLNRLANVVGNRIAEELQRKTNLRGGMA"
     gene            24477..25199
                     /locus_tag="VBD27_000040"
     CDS             24477..25199
                     /locus_tag="VBD27_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7024088.1"
                     /translation="MTKINELTIDGVKTSSFKCDVLVETRPNVIVSSSKTALLEHDGI
                     SGAVVQSNRHRGLIEKPYHITLIEPSDEEIYRFSALLNREKFWLENEQEPTIRLWCYK
                     VDSFEIGKDEFGAWVVDVTFICHPTKFFKTTDTQTLTGNGVLRVQGSALAFPKITVVG
                     QSASETSFTVGNQVIKLEKLSESLVMTNDSDNPSFKTASGKLIKWAGDFITVDTAKGQ
                     NVGVVLGAGITSLKFETVWGWA"
     gene            25200..28874
                     /locus_tag="VBD27_000041"
     CDS             25200..28874
                     /locus_tag="VBD27_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001918212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7024089.1"
                     /translation="MLFLLDKDVRTVKWNGIPLHEASSAIVKEETNSDFYLTVRYPIT
                     DSGIYQLIKEDMLIKAPTPVLGAQLFRIKKPIENDDSLDITAYHVSDDIMKRSITPVS
                     VVGQGCAMALSQMVQNAKTGLGDFSFTSDIMDSRTFNTTETKTLYSVLMDGKHSIVGT
                     WEGELVRDNFALSIKRSRGADRGVVITTHKNLKSYQRTKNSQGVVTRIHARSTFKPDG
                     AEDEVTLRVSVDSPLINSYPYINEKEYENNNAETVEDLRKWAEAKFTNEGIDKVSDAI
                     EIEAYELDGQVVNLGDTVNLKSRKHSADLYKKAIAYEFNALTEEYISITFDDKPGVGG
                     SGVSSGLSNVADAILVASATAQDVAVQRAVKNANAAFDAEFGKTKTKINDDIEIAKAK
                     VESFKSELSNRMDNQLLPLATEAKNLASQAQADLTRKEIELRAELNRQVTSTETVKIS
                     LTNLSHNMDIIKQKALNDLRDAETRLKEADSVQQLATKRVEDKLTGLSTKLESFSVGG
                     YNYVIDGGEPKELMANFYGKTYDINPQLLERTSQATLSFSYEAESTSRLEVRLYKKMH
                     TGDTSKITIIVMPNFDLSPGKGFISQSFDLGGVMPDPRNQAWLVMRGTNANPLTLSKV
                     KLERGTVATDWNNRDETLKASFAEYKQTVDENLANLRTSTETLAGQLTSAESSIRQTS
                     ESFSNRLVSLETYKDSESNRASRYFEASKSETAKQLSALRTEVNGSYVDKSTYEENAR
                     GLTRRFESLSSGTQNYALGTERDVYLNVSGTTVYDLYSFGTTLAIKGIEMGDTVTIAF
                     NYFADSRTAFGSYELELYGHAGKIERVGEVQRTISNRGTYTASFVANNANFRANSLKI
                     RFTDSRLKFRVTTLRVTKGTIPADWSPSPDDLKAYSDTKLEQTANEIKASVTSLDHKT
                     LKQTDITMTSEGIVLRAGKTSNDVARAIGSYFKVTPDAIALFSSLIKVSGNMLVDGSV
                     TSRNLVTGAVETGHVKAGAITGVLLAAEAVTAEKLKVDQAFFNKLMANDAYLKQLFAK
                     SAFITQVQSVTISASQISGGVIKALNNAMEIQMNSGQILYYTDQAALKRVLSGYPTQF
                     VKFATGTVSGKGNAGVTVIGSNRYGTESTNDGGFVGVRAWNGSNIDSLDLVGDKIRLA
                     SSAFDNSDGWDVRTLDSGLKITPHNRAAERNSRIEVGDVWILKGNGSYSSLRDILNSF
                     NENFSKGPNADSYTYYHSGF"
     gene            28891..29121
                     /locus_tag="VBD27_000042"
     CDS             28891..29121
                     /locus_tag="VBD27_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024090.1"
                     /translation="MNQEQQLNQALRLTVNELTAQLANESTTKNLLAIQLTEVDQEKQ
                     QLTQQNAELQARVSELEGLLDEQTQPEIIEGE"
     gene            29124..29462
                     /locus_tag="VBD27_000043"
     CDS             29124..29462
                     /locus_tag="VBD27_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024091.1"
                     /translation="MTTTENNLLDLETITEPFDLATALKYMKENGELIRCKNAVNDFY
                     MYRDMQKRPVIVNGRRQFKDVETVWAFNQWGGTTPTINMADLFNLEYYIMTFDENGNP
                     DWTEPHLEDK"
     gene            29497..29907
                     /locus_tag="VBD27_000044"
     CDS             29497..29907
                     /locus_tag="VBD27_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024092.1"
                     /translation="MPPWLIDSTVVVAMVTVLGGLFSTIITTSANRKDQLIKHQYEDI
                     KEDLSGLIDKVKTIDHTTTETKKISEITKDGTLKIQRYRLFHDLTKEISQGYTTIEHF
                     RELSILFESYQLLGGNGEIEALFEKFKQLPIEED"
     gene            29910..30239
                     /locus_tag="VBD27_000045"
     CDS             29910..30239
                     /locus_tag="VBD27_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO7024093.1"
                     /translation="MTQITDIIISSAMGILTILAGIAVQAVKVYLIKKGGEKAVLITE
                     ILAKNAVNAVEQVATETGFKGADKLTSAKAQILAELQKYNIHMSDDDLTLFVESSVKQ
                     MHDAWKE"
     gene            30243..31649
                     /locus_tag="VBD27_000046"
     CDS             30243..31649
                     /locus_tag="VBD27_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEO7024094.1"
                     /translation="MEINTETAIAWMSARQGKVSYSMDYRDGPNSYDCSSSVYYALRS
                     AGASSAGWAVNTEYMHDWLIKNGYELIAENVDWNAVRGDIAIWGMRGHSSGAGGHVVM
                     FIDPENIIHCNWANNGITVNNYNQTAAASGWMYCYVYRLKSGASTQGKSLDTLVKETL
                     AGNYGNGEARKAVLGNQYEAVMAVINGKTTTNQKTVDQLAQEVIAGKHGNGEARKKSL
                     GSQYDAVQKRVTELLKKQPSEPSKAQEVNKPTETKTSQTELTGQATATKEEGDLSFNG
                     TILKKAVLDKILGNCKKHDILPSYALTILHYEGLWGTSAVGKADNNWGGMTWTGQGNR
                     PSGVTVTQGSARPSNEGGHYMHYASVDDFLTDWFYLLRAGGSYKVSGAKTFSEAIKGM
                     FKVGGAVYDYAASGFDSYIVGASSRLKAIEAENGSLDKFDKTTDIGDGSKDKIDITIE
                     GIEVTINGITYELTKKPV"
     gene            31856..32041
                     /locus_tag="VBD27_000047"
     CDS             31856..32041
                     /locus_tag="VBD27_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEO7024095.1"
                     /translation="MTERELKKIAKKQGFSKTNFGKGSHEVWKHPDGRTVTIPKPKEK
                     DYRPGTLNNILKVLYGE"
     gene            32102..32506
                     /locus_tag="VBD27_000048"
     CDS             32102..32506
                     /locus_tag="VBD27_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEO7024096.1"
                     /translation="MKYTYLALFEVDKENGGYNISFPDFPGAFSEADSLNEAIFNARE
                     VLEIYTIMFEDEGKEFPKASSFKALASNLASDEDVIQAISVDTELVRERERSKIVNKT
                     VTLPSWLVEVGKENKVNFSQLLQKAIREELQV"
     gene            complement(32720..32790)
                     /locus_tag="VBD27_000049"
     tRNA            complement(32720..32790)
                     /locus_tag="VBD27_000049"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32756..32758),aa:Cys,seq:gca)
     gene            complement(32924..34456)
                     /gene="ahpF"
                     /locus_tag="VBD27_000050"
     CDS             complement(32924..34456)
                     /gene="ahpF"
                     /locus_tag="VBD27_000050"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEO7024097.1"
                     /translation="MVLDKEIKAQLAQYLDLLESDIVLQTDLGDNDNSQKVKDFLDEI
                     VAMSDRISLESTHLKRQPSFGIAKKGQESRVIFSGLPMGHEFTSFILALLQVSGRAPK
                     VDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIEGGMYQD
                     EVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGP
                     AGNSAAIYAARKGLKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSY
                     DIDIIKSQLATGIEKKELVEVTLANGAILQAKTAILALGAKWRNINVPGEEEFRNKGV
                     TYCPHCDGPLFEGKNVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKADQVLQERA
                     AKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLK
                     DSGIELNERQEIVVDKFGSTNIPGIFAAGDCTDAAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(34474..35034)
                     /gene="ahpC"
                     /locus_tag="VBD27_000051"
     CDS             complement(34474..35034)
                     /gene="ahpC"
                     /locus_tag="VBD27_000051"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO7024098.1"
                     /translation="MSLVGKEIIEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLDVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQGFDVLGQDGLAQRGTFIIDPDGVIQMMEINADGIGRDASTLIDKVRAAQYIRQH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            35311..36081
                     /gene="rpsB"
                     /locus_tag="VBD27_000052"
     CDS             35311..36081
                     /gene="rpsB"
                     /locus_tag="VBD27_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO7024099.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVAEEAKRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFELLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDVIIPANDDAIRAVKLITSKLA
                     DAVIEGRQGEDADVDFAQEAQADSIEEIVEVVEGSNND"
     gene            36175..37215
                     /locus_tag="VBD27_000053"
     CDS             36175..37215
                     /locus_tag="VBD27_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO7024100.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDLDKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVIEVNAETDFVAKNDQFVTLVNETAKVIAE
                     GRPSNNEEALALTMPSGETLEQAFVTATATIGEKISFRRFALVEKTDEQHFGAYQHNG
                     GRIGVITVVEGGDDALAKQVSMHVAAMKPTVLSYTELDAQFVHDELAQLNHKIEQDNE
                     SRAMVNKPALPFLKYGSKAQLTDEVIAQAEEDIKAELAAEGKPEKIWDKIVPGKMDRF
                     MLDNTKVDQEYTLLAQVYIMDDSKTVEAYLESVNAKAVAFVRFEVGEGIEKASNDFEA
                     EVAATMAAALEK"
     gene            complement(37374..37835)
                     /locus_tag="VBD27_000054"
     CDS             complement(37374..37835)
                     /locus_tag="VBD27_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HEO7024101.1"
                     /translation="MNYIITFLISMIPLVELRGAVPFAIANGIPLWEALAIGVVGNML
                     PVPIIFFFARKVLEWGADKPYTGKFFTWCLKKGHSGGQKLEKVAGEKGLFIALLLFVG
                     IPLPGTGAWTGTLAASLLDWEFKHSVIAVMLGVILAGCIMGTLSIIGFNLF"
     gene            38008..38472
                     /locus_tag="VBD27_000055"
     CDS             38008..38472
                     /locus_tag="VBD27_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEO7024102.1"
                     /translation="MAIKNTSDNIEEYIKSLLEQSGIAEIKRSNLADTFQVVPSQINY
                     VIKTRFTESRGYVVESKRGGGGYIRIAKVHFSDQHQLFGNMLSTIGERISEQVFDDLI
                     QLLFDEEIITEREGNLILATSGDDVLGEQASVIRARMLRKLLQRLDRKGFHL"
     gene            38469..40916
                     /locus_tag="VBD27_000056"
     CDS             38469..40916
                     /locus_tag="VBD27_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO7024103.1"
                     /translation="MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVA
                     GLTFAEYESEVAIEEYEAATILALGRAPKEEITNYQFLEQSPALKKILKLAENISIVV
                     GAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERHAGFTKD
                     DIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDKE
                     ISRMVQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGNIPYELRDMRVLELDMMS
                     VVAGTRFRGDFEERMNQIIADIEEDGHIILFIDELHTIMGSGSGIDSTLDAANILKPA
                     LARGTLRTIGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGLKPAYEAF
                     HNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQ
                     AILDDDMKSASKALKASHKDKKHKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLN
                     LEKELHKRVIGQDDAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKALA
                     EVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTEKVRNKPYSVLLFD
                     EVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGA
                     KDISHDYTAMQKRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILA
                     LKDKGMDLKFQPSALKHLAEDGYDIEMGARPLRRTIQTQVEDHLSELLLANQVKEGQV
                     IKIGVSKGKLKFDIAKS"
     gene            complement(41136..41627)
                     /locus_tag="VBD27_000057"
     CDS             complement(41136..41627)
                     /locus_tag="VBD27_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO7024104.1"
                     /translation="MIIRPVLPSDAKELLAIYAPYVTDTTITFEYDIPSISEFTNRIK
                     NISKSFPYLVAEENGKILGYAYASTYYARASYDWTCELSIYLDKDVRSKKIGSQLYDT
                     LEKELVNQGYVNLLACISLPNDISIAFHKKRGFNQVAHFPKIGFKFEQWHDIVWLQKR
                     LKS"
     gene            complement(41641..42282)
                     /locus_tag="VBD27_000058"
     CDS             complement(41641..42282)
                     /locus_tag="VBD27_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO7024105.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGQHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIFFLNKRFQSIKEAYKANNNVLDRSIFEDELFLTLNFKNGNVTKTELD
                     IYKELLANMLEELEGMPKKRPDLLVYIDVSFDKMLERIDKRGRSFEQVDSNPELYDYY
                     KQVHSEYPEWYENYDVSPKIRIDGNKLDFVKNPEDLQHVLDTIDSELQKLDLL"
     gene            complement(42403..43380)
                     /gene="dusB"
                     /locus_tag="VBD27_000059"
     CDS             complement(42403..43380)
                     /gene="dusB"
                     /locus_tag="VBD27_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEO7024106.1"
                     /translation="MTQLNSSFMIGKVEIPHRTVLAPMAGITNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQNNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPEKIYHIVKEVTSVLDIPLTVKMRTGWSDSSNAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLGKVAKAVTSIPFIANGDIRTVHDAK
                     FMIEEIGADAIMVGRGARSNPYIFTQINHFFETGEILPDLPFEKMLDVAEDHLTRLVN
                     LKGETIAVREFRGLAPHYLRGKSGAAKIRGAVSRAETLAEVQELFAGLR"
     gene            complement(43364..44239)
                     /gene="hslO"
                     /locus_tag="VBD27_000060"
     CDS             complement(43364..44239)
                     /gene="hslO"
                     /locus_tag="VBD27_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO7024107.1"
                     /translation="MDKIIKSISTSGSFRAYVLDCTETVRTAQEKHQTLSSSTVALGR
                     TLIANQILAANQKGNSKVTVKVIGDSSFGHIISVADTKGNVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVVITDYGTGQPYTSTTPLITGEIGEDFAYYLTESEQTPSAVGLNV
                     LLDDEDKVKVAGGFMLQVLPGASDEEISRYEKRIQEMPSISSLLESENHIESLLSAIY
                     GEDDYKRLSEDSLAFYCDCSKERFEAALLTLGTKELQAMKDEDKGVEITCQFCNQTYY
                     FTEEDLEKIINDSIK"
     gene            complement(44379..45635)
                     /locus_tag="VBD27_000061"
     CDS             complement(44379..45635)
                     /locus_tag="VBD27_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEO7024108.1"
                     /translation="MSDTFNFDIDQIADNAITKTDKTTEIISNQTTSQTGQIAFFEKL
                     TPAQKSAISEKTPALVDTFVGDQNALLDFGQSAVEGVNTTVNHILSEQKKIQIPQVDD
                     LLKNANRELNGFIAKYKDATPAELEKKPNLIQKLFKQSKTSLQEFYFDSQNIEQKMDM
                     MAANVVKQEDTLARNIVSAEMLIEDNTKSIENLVGVIAFIESSQAEAANRASHLQQEI
                     LALDSQTSEYQIKSNQLARMTEVINTLEQQHTEYVSRLYVAWATTPQMRNLVKVSSDM
                     RQKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGVTADAIVNANNAALQMLAETSKEAIP
                     MLEKTAQSPTVSIKSVTALAESLVAQNNGIIAAIDKGRKERAQLESAVIKSAETINDS
                     VKIRDKKIVEALLNEGKSTQEKVDES"
     gene            complement(45632..46450)
                     /locus_tag="VBD27_000062"
     CDS             complement(45632..46450)
                     /locus_tag="VBD27_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024109.1"
                     /translation="MVKKRYSKNSHNLITLLGIVVLASLISDFWSEVIATLLIIGGGY
                     CAYYVYDKKRLKCFTSNQRIEALKSDIKETDQDIRHLEILKKDNRSKEYIKLAHQILP
                     QLDLIRNEANQLQKAIEPNIYKRITKKANTFSNEINEQLIKLHASPELEPISDQEDEM
                     IRIAPELKPFYHNIQDDHYAILKKIEEADNKAELAAIHQANMKRFTDVLAGYIRIKQS
                     PKNFNNAKERLEQALQAIKKFNLDLDETLRQLNESDMKDFDVSLRMMQDERNNK"
     gene            complement(46574..48826)
                     /gene="gshAB"
                     /locus_tag="VBD27_000063"
     CDS             complement(46574..48826)
                     /gene="gshAB"
                     /locus_tag="VBD27_000063"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEO7024110.1"
                     /translation="MIIDQLLQRSHSHLPILQATFGLERESLRIHQPTQRVAQTPHPK
                     TLGSRNYHPYIQTDYSEPQLELITPIAKDSQEAIRFLKAISDVAGRSINHDEYLWPLS
                     MPPKVREEDIQIAQLEDAFEYDYRKYLEKTYGKLIQSISGIHYNLGLGQELLTSLFEL
                     SQADNAIDFQNQLYMKLSQNFLRYRWLLTYLYGASPVAEEDFLNQKLNNPVRSLRNSH
                     LGYVNHKDIRISYTSLKDYVNDLENAVKSGQLIAEKEFYSPVRLRGSKSCRNYLEKGI
                     TYLEFRTFDLNPFSPIGITQETVDTVHLFLLALLWIDSSSHIDQDIKEANRLNDLIAL
                     SHPLEKLPNQAPVSDLVDAMQSVIQHFNLPPYYQDLLESVKRQIQSPKLTVAGQLLEM
                     IEGLSLETFGQRQGQIYHDYAWEAPYALKGYETMELSTQLLLFDVIQKGVNFEVLDEQ
                     DQFLKLWHNSHIEYVKNGNMTSKDNYIVPLAMANKVVTKKILDEKHFPTPFGDEFTDR
                     KEALNYFSQIQDKPIVVKPKSTNFGLGISIFKTSANLASYEKAIDIAFAEDSAILVEE
                     YIEGTEYRFFVLEGDCIAVLLRVAANVVGDGIHTISQLVKLKNQNPLRGYDHRSPLEV
                     IELGEVEQLMLEQQGYTVNSIPPEGTKIELRRNSNISTGGDSIDVTNTMDPTYKQLAA
                     EMAEAMGAWVCGVDLIIPNATQAYSKDKKNATCIELNFNPLMYMHTYCQEGPGQSITP
                     RILAKLFPEL"
     gene            complement(48996..>49641)
                     /locus_tag="VBD27_000064"
     CDS             complement(48996..>49641)
                     /locus_tag="VBD27_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO7024111.1"
                     /translation="TIITFLHNVLGDNYEIVLHVVDENDIYIGELVNSHISGRTISSP
                     LTTFALDLIKNKVYKEKDFVTNYKAIVSPLNKEVRGSTFFIKNAQNELEGMLCINLDI
                     SAYQNIALDILDLVNLNVNKILSKSPQKISLPQQEEPVEVLSGNIQDIISEIVDPSLL
                     NQNIHLSQEVKVEIVSKLHEKGVFQLKGAVSKVAEVLNISEPSVYRYLKKIETH"
BASE COUNT        15801 a         8954 c        10311 g        14575 t
ORIGIN      
        1 ataacaaaaa aatcaacgaa caatcgttga tttttttgtt atctttggat aaaatctttt
       61 atatttaagg cggtagacgg atttgaaaac cttggtttat caaggttttt ggttgtttcg
      121 ccccaaattc gccccaaatt atgcgactaa aaaaatattt ttgattttct caaaatttct
      181 ctcagccagt gcttccatct gatgtgtata aactttcaga gtaatatctg gactttcatg
      241 tccaaggagc ttagaaattg tcacaatgtc aaccccgttc aaaatcaaaa acgaagcgta
      301 tgtgtgccgt aatgagtgat ttctgacttt cctacccaca attttcttaa tcagtttatt
      361 gcaagctgaa ttagatacac caaagcacac tctattcttg atattagctt gccaatgatt
      421 tttcttatat tctcgaagaa tatctattgt atttgaatca attggaactt ttctttttga
      481 tgactcattc ttcagatctg caaaatcttg agtattcgag taatcaaaac tcttgtttat
      541 atccaaaatg ccatttttga agtctatatc gctccaggtt agtcccatga cctctgagaa
      601 ccgcaaacca gtgacagaaa gaaggtagag agtgaaatag gatacatact ggatattctc
      661 tcttgtgagg gataaaagag ccttatactc actttcttct aagaagtcat tttcctcagc
      721 tcttgattca atttgtgatt taacctttgc atcatcagca aagttaaaat gaataacttg
      781 ttccctcaca gctactttca tagctccctt gatttggtag tggaacttct caagagtttc
      841 ttgagcatat ttctccccaa attcatttag ccgtttttga taatagagtg gtgttatatc
      901 cttaaccttt atatctttaa aataggtttt gatgtgtcta aagttctttg tgtaggtttc
      961 ccaggtctta tcctttacat aaggacgttt ataaacttct gaccaagttt tgacaaaatc
     1021 atatagagta acttctccat cggtcaggat attttgagat agttttttct caatatccct
     1081 agcagctgct tgagcaagtt ttttggtttt gaaaccactc ttagactttt gacggaactt
     1141 accgctctca tctttatatg aaatgcgata ttcccagcca ttatcacgtt ttcgataata
     1201 agccatttgt ttttcacctc attttttgat aaaatgggta tagtaaagag acctactgct
     1261 aaagcaggtt tctcactatc cacaattgcc ttacgcttgc accgaccaaa gttgagcgta
     1321 gggctttttt tattttttaa ctttattttt caacgcattc tcgatagcat ttttaagctc
     1381 aagaatagct attttctcat ctttactgaa ggttactgta ttttcgtctt ttactgcatc
     1441 gaaaacaccg cccttagtat cagatgaacc aggataaacc aattgtagat agccaacagt
     1501 tgaactaggt tccttgagtt gataagctgt aatttctgat aatagaattg acttttctcc
     1561 atctaagcca tgtagcaaca tatttgacat gttgcttttt cttgcgattc tgatgaagta
     1621 atcatcaata cgtacaattg tttttgattt tttgaattca aaaacacgtt cgttaggctc
     1681 tgatgtaaag agttctactt caatttcttt atcttttccg ccaaataatg ccattattgt
     1741 tgtctccttt tgtttttatt gtaaccgtaa aatgttaaaa atctgccctc caccttgacg
     1801 gtagtttaaa actaacttga gggcgtagct ttcagcatac tgcaagttat agatgagata
     1861 gttcatcagt ctgttgtgta gtgctggttt gctaatatct gtttcacgtt gaatatcttc
     1921 aaagctctct ctagttgtga gggctttttt gagtcgttca tctgttaggt agaggattga
     1981 agccactgta tcagcttctt tctcaattgg aatcagctct ttcgggtact tctcactagc
     2041 gtttttactc atgagagaca tatagattgg ctgattttct gtttgattaa ggtgacagtg
     2101 tatgtgacta agttcgtgta aaatcgtgaa gataacacgt cctttggtgt ttgtatgttg
     2161 attgatatag atgatgtagc gtccaaggtc taggtcaggg acagtcaatc cagcacagct
     2221 ttcgcagagt accctatcag tataagtaac ggttctattt gataccagcc ctctgtactt
     2281 gatgtcttta tctgtgggat gataatcagg taattctgga taacgttctt tcatctcatc
     2341 gtaatcaaga aagttaaaga gaataggata gttttgttca aaatgtttaa tgacatcttg
     2401 ataactaacg ttctctttca ttttatcaat tgagcttatt atttgataag ccttattgtg
     2461 atagtcaaaa tactgttccc ttgatagcgg tgtgtatttc aataagtcac ctacttccag
     2521 ttagaatcat cttgaatcaa ttgtctagct gtcttcatta gaccttcaag agcagaattg
     2581 aaccgctctt tttcagaagc tgaaagtcct tctgtttcct tacgaaaaag aacaatagct
     2641 tgctgagtta gatcatcaac tgcagtatta tcttggccat cggcaatctt aggattttct
     2701 gttctaccga gaaggtagtc agtgctgaca ttgaaataat ctgctatttt tgagacacgc
     2761 tcaacatttg gtgtagattt tttcatgttg tatatggtat ttctaggaaa atccaactgt
     2821 tcttcaagtt gattgagtga aataccttgc ttgtcagcca attccttgat cttttcaaat
     2881 gtaaaaaaca ttgatttatc aacctttcta aggcatgaca aaaaatattt aataaattta
     2941 ctacaaaact gttgacaggt tttaataaat ttactacaat atcgtttgta agctaagtcg
     3001 ttagcgaaca agacaagtaa aaaataaagc ctaaacaact gattggcgtc ggttttatgg
     3061 gttaagcctt agctttttag caggtctttt ctctatgctt tgattttaat aaatttattt
     3121 attgttgtca agaagttcgc taacttttta gcaaaaataa taaaaggagg gtagatgaat
     3181 gggaatcggt tcaatgacag tgacagttaa agttactaac atggataaat ttattgaact
     3241 tagtaaagag ttcaataaaa aagctcgcga gctagaagaa ctagctcacg aactgaagtc
     3301 atttgatttt gaaggcgaag ttgtatcaac tgatagcaac tagctcaaat gattcatcat
     3361 ctgaaaaaag ataaacaggg tattcttctt tagttgcaaa attgaagaaa tcatatccaa
     3421 aattatcttt tttgatgtgg gaattttttt cggagagaga gtcaagattg acctgagata
     3481 gtagcttaaa gatttgcaca cgagcttgtt cagcatcgtt aactctttgc aaggctctca
     3541 agtgctcttc gctagggaaa gctgctatca aatcagctct gtcatctatg taagttctag
     3601 tgtcatcaag atagccatat tttagattat agtctacaaa tgaccgctgt tcatattctt
     3661 gataggtttc aaagctgaca aggttacctt tgtaacgata gacgagaacg tcatcttgta
     3721 actcttcttc gaaatttgtg atgccaatta gaaatttatg gcatgcctct atgatacgaa
     3781 tttgattcaa attcatggct tgacctcctt tcataaaaga tttcaagtca attataccaa
     3841 actagaaagg aaaaatatga gtcaacaaca gaaaaaatgg attcagctag tgaaagataa
     3901 gctgaatgaa gagcaaatga cacagacaca tctagcccgt gcttgtggag tcgcaaaagc
     3961 gacaatctca gagctactaa aatacggaaa aggtagtgtc cgtctgaaaa ataaagtctc
     4021 agacattctt cacattgatg aaagctggac ggacttggag gaagattgat gccgataaca
     4081 agagaaatga caatcactga aatcaatgtt ctcaatgcta tcaagaatag cgctacttat
     4141 gatcttccga ttcaagccag agagctaagg cagcagctag gacttagcaa acgatcactt
     4201 gaagcagtca ttgagaacct gagagtgatt tacaagcaac caatcgttgc caaaaaaaaa
     4261 cagcctagcg gatactatct tccaagaaat gaagaagaaa gaaacgacgg cttagcccca
     4321 tacaaaagac agatattgac tgagcaaaat aatcttgctg ctattatgtc tgtcaatctg
     4381 gaagaatatt gggcaacaga aaaagcctga cggcaatcag gctcacaaaa tattattaca
     4441 agaggattat atcacaatga acgaagtttg gaaagatata ccaattgtta aaggatatta
     4501 tcaagtttcc aatcttggtc gtgttcggtc tattggtaga acagtcaatg caaaacagag
     4561 aacaagaaaa acaaaaggac gcatcttaaa acagagctta tcttcaggct atgcaatagt
     4621 gacactttca gtcaatggat taaggaagtc gataagagtt catagactag tagctgaagc
     4681 atttatacca aatccaatca ataagcgaac gataaatcat attgatgaaa ataaacttaa
     4741 taatagagta gataatttag aatgggcgac agataaggaa aatgctaatc atgggaatag
     4801 aacaacgaaa tcatctttag gtcgttgtaa acctgtagaa caatttacac ttgaagggga
     4861 attcataaac acgtttgact caataaaatc tgcatcaatg aaaacaggta tctcgtcaca
     4921 aagaataaca gcaacagcaa tggggcacca aaaacagact cacggatata aatggaggta
     4981 cgcataatga atgatctaat gattcaaatg cttgaccagt tcgaagctgg tctaatggac
     5041 agaacgatga aagtaatgca cgttgtgaca gacgaaaagt atagatttcc tttagaactc
     5101 aacaagtctc aatgcgcttt aatgctctta ggtactaaag atactgggac atttgatgaa
     5161 cgctttaact gtcataagga ttttccgagg attccaaatg ctcgtgagaa ataccctcgt
     5221 gatgaagtca ttgaatggta tcacaagaat tggcaaagaa ctgttatcta agaaagaaca
     5281 aaaggaacta tatcatgaac aatttacaaa ttatcgcagt aggcacactt atttcagtaa
     5341 ccctgattga gtctcttatc tcaaatatca agctcagaaa agctctcaag aacaaacaga
     5401 cagctgggac tgtcattgct caatatatcc cacaacgagg cttcattgac tacaagactg
     5461 gaagacgtgt tgacattgac cctgaaacaa agaaagaagt ctttgttgac tgaggaaagc
     5521 tatggatgaa ttaaaagtat tgcctcacga tatccaggct gagcagtcgg ttcttggttc
     5581 aatttttatc aaacctgaaa aaatgattga ggtggctgag tatctgaaac caaatgactt
     5641 ttacaggcca gctcacaaga ttctatttaa ggccatggtg agccttgctg atcgtggcga
     5701 ggccatcgat attgtcacaa tcaagtcaac gctggaaagt acggatgaac ttggcatggt
     5761 tggcggtatc agctacatcg ctgagattgt taatgcagtg ccaaccagct cacacgctga
     5821 gcattatgcc aagattgttg ctaaaaaagc acagctacgg tctatcattg acaacttgtc
     5881 tgactcaatt ggcaatgcct atgatgagga catggatatt gatgagatta tcgcaaaagc
     5941 tgagcgatca cttatcgaag ttagtcaagc cagtaacaag agtagtttca gacctatcca
     6001 tgatgtcctt ttggaaaatc attcaaagat tgaggaacgc tcaaacaata ccagtcaaat
     6061 cactggtatt gaaacaggat tttatgactt tgacaagctg ataacaggct tgcatgaaga
     6121 ccagctaatt gtcttagcgg cacggccagc gatggggaag acagccctag ctcttaacat
     6181 agctcagaat gtggcaacta agtccaataa ggctgtggcc gtcttctcgc ttgaaatggg
     6241 ggctgaaagc cttgttgagc ggatgttgtc agctgaagga acaatcatca accatcatat
     6301 tagaacagga aatttgacgg ttaatgagtg gcaacgtctt atctatgcac agggtcagct
     6361 tgctgaagct ccaatcttta tagatgacac agctggagtc aagattactg acatcagagc
     6421 aagagctagg aggctatctc aagagactga cggacttgga ttgatagtca ttgactatct
     6481 tcaactcatt caagggtcac ggtctgacaa ccgacagcaa gaggtatctg agatttctag
     6541 gcagttaaag attattgcca aggaattaaa agtgccagtc attgcactat cacagcttag
     6601 ccgtggggtt gagcaaagaa acgacaaaag gcctatcatg tccgacttaa gggaatcagg
     6661 cagcattgag caagacgctg atattgtcgc cttcctctat cgtgatgctt attaccaaga
     6721 caaaaaagaa ggtcagccag aaaacgacat cactgagctg atcatcagaa agaacagaca
     6781 cggaaacctt ggcacagtca agctgtattt ccacaaggaa tataccaagt tttctagcgt
     6841 tgaggaggaa tgaaatgaaa aaatttgacc actatttcag caaccatatt gctgtaaaaa
     6901 acgaatctga tacagtcacc attgttgact attacaatcc agaaaagggc tatgagtata
     6961 acttacgtta cattttcgat agaaaaaatt catcccttgc tatcacagga gactttgggg
     7021 aattagttgc acgaaatttc tacaatatgg gtgaatggga cacattctac aatcactata
     7081 ctggtcatct ggggtatttt cttgaaaagt tactatgttc aagtaggcca gttcattact
     7141 atgatactga caaggctaaa agagaaattc tcaatgaatt ctttgatggt gctgattatg
     7201 atgatttaga ttttgatgat gaattcctac ttgatcaatt atttgacaat tttgacgaag
     7261 ataaaggatt tacttatatt cttccagagg cttttgaacg ttttagcgaa gatatggaag
     7321 aatatgaggt ctatgaattt ctatcaaggg ctggccgata cgtcaatgat gtctttgaac
     7381 tttacttaga cgcttataag agagcttatg agtatctaac aaaggaggtt gatgatggca
     7441 caacgcagaa tgtttagtag aaaaatcact gagactgacc gttttttgga aatgccacta
     7501 tcatcccaag ctctctattt ccatttgaac atgggagctg atgatgaggg ctttatagat
     7561 aaggcaaaga cgattcagag aactattggc gctagtgatg atgatatgaa attactgatt
     7621 gcaaaagggt tcttgattcc ctttgatagt ggtgtagtcg ttatccgtca ttggcggatt
     7681 cacaactata tccagtctga ccgctttcaa tcaaccttat accagtcaga aaaggctcag
     7741 ctagagtatg acaagtcaaa aacatctagt cttaaaccta ttggaaattg tatacaaaat
     7801 gtatccaaaa tggagacaca ggttaggtta agtaagggta gcttagataa ggatagctta
     7861 actacctatc ctacagtttc agacaatgag gaagaagata ttccatacaa ggaaattatt
     7921 tcttacttga atgaaaaagc taatagaaat tacagaccta acattcagaa aaacaaaact
     7981 cttatcaagg ctagatggtc agaaggattc aggttagatg atttcaaaca tgtgattgat
     8041 accacggtca aggattggtc aggcactaag tatgaaaagt atctcagacc tgaaacgctc
     8101 tttggttcta agtttgaagg ctatttgaat caagcaccac gaatcaagac ggaaacaatt
     8161 gacggaaggt tgggctttta gatgaatcct tttaagaatt ttgaaactag gcaagtcttg
     8221 gaagaaacct gcgaggttca cggttgccag ctctggctga ctaaagtacc gattaaggga
     8281 cggcttgaag aactcaagca gtgtccagag tgtaccaagg ccgctatcaa catctttgaa
     8341 aataagttga acagtcagag caaaatcaac agcaagcttg ctgatacata tgctgtcttt
     8401 gaaagagata gcttggtatc agacaagcta agaggcaaga gccttgagaa ctatgaaatc
     8461 aaggatgata ttgaccagca agctatcaat tatgctaagc gtatggaaca gttctaccga
     8521 caggatagaa caggcaatgc cattgtaact ggcccttctg gtgttggaaa aagtcatctg
     8581 acttacggct tggccaagtt tatgaacgaa caattcaaag cctacgaatc accaaaatca
     8641 gtcctcttca tctcactggt gagccttttt accaaaatca gagaaagctt caaagctgac
     8701 aatgggtacc gacaggctga gatgattgag ctgctaacta gtgtggacta cctctttctg
     8761 gacgatctag gcaaggagag tcgcaaaggc gacagccaga acaatgagtg gactcatcag
     8821 attctctatg agattttgga caatcggagt aacacaatca tcaacacgaa tctgagcagt
     8881 aaggagatta aggccttata tgctgacaat tacggtaatg gtgctctttc tagtcggatt
     8941 cttgagggag tgactggcaa tagctttgct taccctaagg atatggaaga taggaggtat
     9001 tgatgagcta taaagccaca ttcatgctat atggttacaa agctgttccg attggtctat
     9061 ttgatacaaa ggagttggcc gctcaagcaa ttgttgacca catcattgaa aactctggaa
     9121 tacctagacc cagggtagtt gccactatga aaatccgaag caaggaaatc agaattgatt
     9181 atggttctaa aacttgctac tacttgatca ctttaattaa taataaaacg gaggaaaaac
     9241 aacatgacta aaacaatgac acttgaagaa aaggttgaac agtggtttat tgaccgtaac
     9301 ttacatgagg ccaatcctgt taagcagttt cagaagatga tggaggaaac tggggagctt
     9361 tactctggta ttgctaagga caagagtgaa attatctatg attcgcttgg agatatgcaa
     9421 gttgtcctga ttggcattga acaacaaatc aagaatggcg ctcagattga agcaagtcca
     9481 caagatatgg agttgttgct attggcttca agtttggggg aaatggcaca gaaactctac
     9541 aagcatatct tccacaatga aacaaaaaca ccgcttatcc gtccagaatt aagcttgctt
     9601 cactctacta ttcatgcaat agctatccac aacttgacaa ctgctgatga ctgcctatca
     9661 atcgcttatg aagaaatcaa agacagaaaa ggcaagttga tcaatggcat ttgggttaag
     9721 gaggaggatt tgaaatgaaa aacatgaatt ggaaagcatt cttcaacaat ctcaaggcct
     9781 ttgcaagtgt catcatcatt actgtatttg ttcttggatt caactatctg ttttatgaga
     9841 ttggctacca gaaaggccat caggaagctg acagggtgat tatctatgtc gctgataatg
     9901 ctggcgctga gatgtttggc aaaatcactg ataaagaaat cattgaaggc cgtcacacgg
     9961 ttacagctgg cgcttatgga aaatttctgg tcactgagga acaatacaat gaaatcaccg
    10021 ttggtgatga cataccagac tacctgaaag gacgtggaaa ctaatgacaa aatgtaattt
    10081 tgaagcagga cacaaaccaa ttgacacaat tggacaacta gccaaagaag tccttgatgg
    10141 gaaatatggc gatggtgagg aacgaagaag aaatctaggc tgtttatacg atctagttca
    10201 aacaagagtt aattatatgg ttcatgaacg tgacagcaag ttcacaggta tcaagattta
    10261 cgacaatgtg aacaaaccta gccattatca aggaaagaat ggcatggaag ccatcgatgt
    10321 tgtcagaaat ttcattggac agctcacagg agtatctgct ttctactggg gaaactcaat
    10381 gaagtacctg ttacgcttcc agggcaagaa cggccttgaa gacctcaaaa aagcaagaaa
    10441 gaaccttgac tggttgattg aagaaatgga gaaagagaaa tgaaaaaatc aataggaatc
    10501 cttgaattta aagaatttga tgcttttgtt ggtggaaagg ttatgcctgt ctatcttcat
    10561 agtgctaacc caaacgtgcc aacgactaat aaaaatgagg ctatgagggt taccaaaaag
    10621 agatacagaa aagagttctc tgacaagtac attttcagaa aggttggtga ggttgatgat
    10681 caataatgtt gtacttattg gccgcttgac aagagatgtt gagctacgtt acacgccgtc
    10741 aaacatcgca aacgctactt ttaacctagc agtcaatcga aatttcaaga atgctgctgg
    10801 tgatcgtgaa gctgatttca tcaactgtgt gatgtggcga caacaagctg aaaacttggc
    10861 caattggacg aaaaaaggaa tgctgattgg tattactgga cgaatccaga caagaagcta
    10921 cgaaaatcag caaggtcagc gtatctatgt gactgaggtt gttgctgaca gcttccagat
    10981 tcttgaaaag cgtgataatt caacgaacca ggcaagcatg gatgaccaat tgccaccatc
    11041 atttggaaat agtcagccta tggatatttc agatgatgat ctaccgtttt aggaggtgct
    11101 tatgaacgaa tataagaaac ccacctacat cgtcattcaa gaggcaatgg ctgagcgtat
    11161 tagatttctt gaagatgaat tatataacag agcatacaaa gacattgaac ggctagaagt
    11221 tgagaatgac cgtctgaagg ttagatgcct tgatttgcag ttggaaaatg ctgactatgt
    11281 ctgggatgac atgtgtcgct ctgctattgc tcgtgcaaaa tcagaaaatc gaagaccaca
    11341 cagaagatgg aggggaagat gaagttaagg cttgaagaac taagaaacaa acttggcctc
    11401 agtcgcagag atgtgcatga agccactggc atttctatga acacgctgct acattatgaa
    11461 aaaggcggta caccatctat tggtcaggtt gaaaatatcg ctaaggctta caatatcaac
    11521 ccagcctggt tgcttggttg gattgatgaa ggccagcaac cacaaccact tgaaaccgtt
    11581 gttgaaaaga ttatctatgt ggaaaaggaa ggctgtagat taccgccata ttggaacaat
    11641 gataataatg gcagaatcat caagtggaaa gaatcaagaa gagcattcca aaggagaaca
    11701 atttgacaaa gaaaacagca agtaagacaa gacgtgattt ccttgagttt gagcttgagg
    11761 ctaagtatct aaaaattgat aaacttattg ggcagcgccg tcatgaactg gaaagaatct
    11821 atgcagtcaa aaacctgaca atgccaggaa tcgatgattc aggagctagt cgcagtggaa
    11881 cttcaactaa tacttctgaa aatctagctg ttgcttatgc cagtgaccca atgattctga
    11941 aactggaaga gttccaaaat gcgatatcag aattactaga ggtacttgaa cccgatgata
    12001 agaaaatttt ccatctgagg tggggagaac atactggata tgattggatt caagtttggc
    12061 acatcatgga aaatggcgag acaggttatc tttacagaca tagcaagcag atttatagaa
    12121 gacgtgaagt catccttgat acacttgcaa aattattgtt catgtaactt gtcaaaaaaa
    12181 catatagaat tgacagaaac aatctgatag attgatagtg tcgctaagca ccgagaaatc
    12241 cttggtgctt tatttttttg agaaaggagc aaagcgatga atattgttga accgctacga
    12301 gacaaagacg atatacaagc catgaaggac tatctatcat cttggaatga aaagtattac
    12361 atgctatttc ttttgggcat caatacaggt ttccgtgttg gagatattct caagctaaag
    12421 gtcaaagatg tccaaggctg gcacatcaaa gttagggaac aaaagactgg taagtataaa
    12481 agtattaaga tgacaaggcc actcaaaaac gaattgaggg aatttgtcaa agacaaggaa
    12541 cttcatgagt atctatttca gagtcgtgtc ggaaagaaca aggcgctcag ttataagacg
    12601 gtttactggt ttcttaaaag agctgctgaa gacctcggca ttgacaatgt tggaactcat
    12661 acaatgcgaa aaacctttgg ctatcattac tacaagaagt acaagaacgt tgctgacttg
    12721 atgtcactat tcaatcattc aagcccagca gttacactaa tctacatttg tgtaaggcaa
    12781 gatgagcttg atactaagat gagtaatttt agcctctaat atttttttga ttttttcaac
    12841 tatccataac gaggaagttt ctagtctata ttttgaagtg agcttaaagc cttgtcctaa
    12901 ttagttttct agtgtgaaac aaaattggat aaaatataag atatagatag ttcagcatgg
    12961 ttattttaca taatttcagc catagaaaaa taatcttgtc aaaaaaatgg gtattattga
    13021 caaaaacaat ctgatatatt tgtaacatga gaaaaatcca gagagcaagc ttaggcatgt
    13081 tctttttttt gttgggggtg acgatgccat gaaagatgaa aatgcatttg atggaattag
    13141 gcttgaagct ggtagcactt tagaaatcta tctcacgaag aacgacttag aacatattgc
    13201 aaatggatat gaggtgactt tagatattaa gcctaacgag actgtaaata aaatcgtaat
    13261 aaagccaagt tttgtaaata atattctaaa tccgttaatc aactatgaca aaagaatagt
    13321 gagtgaagca gatttaaagt ttagagacat ttcaagagag gttgcaagag atagttttgc
    13381 attggggtca atgtagtggg ctatagaaac cctaaacact ctgactggtt cagagcttgg
    13441 cagattaaat tctacaactc gaaaccttgg agaactctga gaaataaaat cagaaaagct
    13501 aagcgtatgc gttgtgacat gtgcggacgt ttgattcacg gtaagagtat tgttgaccac
    13561 atcatagaga ttgacgaaac taattatcaa gatgaatcta tcactctcaa cgaagagaac
    13621 tgtcagttac tttgtcttga atgtcacaac acaaaaactt ttcaaagtaa aataaattta
    13681 aatttagata atcggaatat aaatttattt ttgatttttt tatttttgaa tttttgtgga
    13741 ctccccccat tttgaatttt gacagcgcca aaataataac ggtgtcaatc ctcttgtgta
    13801 cctctccccc aaaattgacg aaaattgata caagaaagga gcatgatttt gaaaatcaat
    13861 gaagttttag aaaagctagg aataagtcgt gctaccctta ccaggtatcg aaaaaagctg
    13921 ggcatatttg aagaaactag gtcaaatatc accaaaagtc agttcaaaga gttggaaaag
    13981 cttgccaatc aacggcaaaa gtacacaagg caggaacgtg ttgagctatc tcgtaagact
    14041 ttcaagctga ttccaaaaga aaaaatgctt gaaatcagtg ataatgattc agttgtgttg
    14101 aaaaatctca aaactcaata caatcacaat caaaaagtga ttgaaaactt ccagcttgaa
    14161 atcaataagg tcatcaatga cggtgagcta cctgataagt acctacttga tggaatggaa
    14221 aagtatcaaa agcttaacat gcagatcatg tcaacgattg aaaagcaaag tccacagggt
    14281 gacagtctca aagaaatgat tcaggagaag ttagcacgtt atggttgaga tgagatattt
    14341 tgataagtat gctcagctca tctatactgg taagattcgt atttgtaagc tcacaatgaa
    14401 atcaattaga cgtgttgagc gatacaaaga gcaatacctc ttcaaacagg aggaagctga
    14461 caaacggatt gagttcattg aggaagagtg cagcaatact aaaggccttg ctggtaagtt
    14521 acgcttagca ttaccacaaa aggtttggtt agaaacaacg tggggctttt atcacacggt
    14581 tgaggttact aagaccaatc ctgatacctt ggaagaatac acagattatg aagaaaggcg
    14641 tctcattcat gaggtgccta ttattgtgcc tcgtggcaca ggtaagacta ctcttggttc
    14701 tgctattgct gaggttggtc aaatcattga cggtgagtgg ggtgctgata ttcagcttct
    14761 tgcttacagt cgtgaacagg ctggctattt gttcaatgcc tcaagggcga tgttgtcgaa
    14821 tgaagaaagc ttgctgcact atatgcgtga ggctgacatc ctacggtcaa ccaagcaagg
    14881 tatcttgtat gaaacaacta acagtcttat gtctatcaag acttctgact atgaaagcct
    14941 tgacggtact aatgctcact acaatatctt tgatgaggtg cacacttatg atgatgactt
    15001 catcaaggtt gtgaatgatg gttccagccg taagcgtaag aattggataa cctggtacat
    15061 ttccacaaat ggaactaagc gtgacaagct ctttgataag tattacaaca tctgggtaga
    15121 tatccttgat gacaagatta tcaatgattc tgtcatgcct tggatttatc agttggacga
    15181 tgtgtcagag attcatgacc ctgatatgtg gcagaaagct atgccattac ttggtatcac
    15241 gacagagaaa gaaaccatcg ctcgtgatat tgagatgagc aagaatgatc cagcacaaca
    15301 agctgagctg atggctaaga ctttcaatct tcctgtcaac aactatcttg cttacttcag
    15361 caatgaagag tgtaaaggtt ggtcagataa gtttgatgag agtttgtttg tcggagatga
    15421 tgaacggaac gcccgttgtg tgattgggat tgacttgtca gatgtcaatg acatctgctc
    15481 tatctctttt atggttgtgc gtggggaaga acggcactat ctaaacaaga aattcatgcc
    15541 acggcatacc attgagacat tgccaaagga actgcgtgat aagtacacgg agtgggaatt
    15601 aagtggcatg ctgcatgtgc atgaattgga ctacaatgac caagcctata tctttgaaga
    15661 gttacggcag tttatgagtg acaacagaat tttgcctgtg gcagtcggtt atgaccgcta
    15721 caatgcaagg gaacttattc gcttgtttaa cgactactac ggggatattt gtcacgatat
    15781 tccccagacg gtcaaatcgt tatcaaatcc gctcaaggtt tacaaggaga aggctaagat
    15841 gggcaaaatc atctttgatg atcctgtggc gacatggaat catgccaacg tccgtgtcaa
    15901 aattgatgcc aataacaata tttttccaaa caaggaaaag gcaaaagaaa agattgacgt
    15961 ctttgctagt cagctagatg cctttatctg ttatgaaaat ttcaaggaag acttgagcta
    16021 ctactttgat tgaggtgaag aatgaacaac tatttgaaaa atttgaagga ggtttttgct
    16081 aggattttcc gtccaaacaa tcggaaatct acaagaacct atcttcaaag aagtatctcc
    16141 tactggcgta ggaactccat ctatttggat aacatctaca ataagatttc aactgacaca
    16201 gctcagttaa ggtttaagca tgtcaagatt actcgtaacc caggcggtgt cgattcgatg
    16261 gtttggtatg agcatagtga tttggctgag gtgctaacag tttcaccaaa tccactagaa
    16321 gtgcctgttg tcttttggtc aaatgtaact agggccatgc tgcgtgacgg tgtggcggtt
    16381 gttgtaccac gttggaagaa tggccgactg gttgaaatat ggcttgctaa gaagacagtg
    16441 acttggacag cagagagcgt ggagcttatg cttgatgatg ttgctgttga gcttcctctt
    16501 actgatgtgt gggtatttga aaatcctaag ttgaatgtca cggctcaact caatcagatt
    16561 actgagctta ttgacattaa cttgaatgct ttgacagaaa agcttagcga tggcaattct
    16621 agcttgagag gtttcttaaa gctaccaact aaggcagctg atgaacattt gaagcaacaa
    16681 gccagggacc gtgttgatag tatgcttgac ttggccaaga acggtggcat tgcttatctt
    16741 gagcaaggcg aagaattcca ggaacttagc aaagactatt ctactgcttc taaggaagaa
    16801 ttagagttct tgaaatcaca gctttataat gctcatggta tcaatgagaa attatttacc
    16861 tgtgactata ctgaggaaca atatagagcc tactattcta gcgtcatgaa gttatatcag
    16921 cgtgtctatt ctgaggagat taacagaaaa tacttcacga agacagcacg gactcagggc
    16981 aataagttac tggtcttctt tgatatggct gacatgattt catttaagga tttggttgaa
    17041 ggtggcttca agtccaaata tgctggattg atgaactcaa atgagttccg tgaaacctat
    17101 cttggtttac ctggctatga gggcggtgaa gtatttgaaa ccaacctgaa tgctgttcgt
    17161 atcggtgcag aagaatctga gtagaatcta aagggtgggt ggttggcata tctttcacga
    17221 aaggaggtaa gcaatggaaa aactaaaaac ctttgttgtg aagtcggttg aggatgaatc
    17281 agctgacttt cattttgagg cttacgcttc aacctatgac aatacagaca gagaaggcga
    17341 tgtgatggcc aaggggtgtt ttgataacac tctgaaatct aaggctgttg tccctatgtg
    17401 tcttaatcat gaccgtaact gtgttattgg taagcatgaa ttgtctgtgg atgaaaaagg
    17461 cttgcgaaca cgttcaacgt ttaatcttag tgatccagag gctaagaaaa cttatgacct
    17521 catgaaaatg ggggcattgg atagcctgag tattgggttc tttattaaag attatgagcc
    17581 tattgacgct aagcaacctt atggcggatg gattttcaag gaagttgaaa tctttgaaat
    17641 ttctgttgtg accgtgccag ctaaccctca agcaactgtt gacaatatta aggaatttga
    17701 tatgtcggcg gttgataagc gaatcgctca ggtgaacatg aggcaagaaa tcatgagtaa
    17761 acttgcaaaa atttaaagga gacatgatga aaaaatcact cattgagctt ttggaagctc
    17821 gtcaaaaagc aactgatgaa ctggctgagg taaagcttaa aaaagccact attgaagcaa
    17881 agatgaaatc ttcaaccatt gaagacgatg acttggaaca gttaaaaact gatgcagaaa
    17941 gtttggtttc tcaagcaaca gctattaagg aaacaattgc tgggctggat tctgacattg
    18001 aagaaactga agaagaactc agcaaagctg ctaaaatcat taaagaaaaa cagaaaggta
    18061 atacacctat ggattatttg aaaaccaaag ccgctgctct tgactttgtc cgtattctga
    18121 tggacaacga aggtagcgca aacagtgccc gtaaagcgtg ggaagctaac cttgttgaaa
    18181 aaggagtgac taacctcact aagattcttc cagaacctgt tcttatcgct attcaagatg
    18241 cctttactaa ctataatggg atcttgaacc acgtttcaaa agatccacgt tatgcggtgc
    18301 gtgttgctct tcaaactcaa gtatctcaag ctaaaggaca caaagctggt aagactaaga
    18361 aagatgaaga cttcacattc ttggatttca ctatcaactc tgcgacagtt tatatcaaat
    18421 atgcctttga atattctgac ttgaagaaag atacaacagg cgcttacttc aactatgtga
    18481 tgaaagaact tgctcaaggc ttcatccgaa ctattgaacg tgctgttgtc attggtgatg
    18541 gaaaatctaa cagtgctgaa gacaaaatca ctgaaatcaa atcaattgct gaagaaactg
    18601 aagcgaatct ctttgaaaca caagaaatca atgttgctgg tgtatttgac aatgctgttc
    18661 ttgaaacgct ggttgctggt attgataaga tggttccaaa tactactcca attcttgtca
    18721 cttcaaaagc cattgctcgt aaattgaaat tggtcaaaga tgcagaaggt cgctacatcg
    18781 atcctcaacc atttgctcca attgcaacca acggaaatgt tattgctggt tttcaagttt
    18841 acatctatga ctggatggac ggtgcaacta acccaatcat tgcatttgct gaccaagctt
    18901 ataagatgat tggtgatgag gctgccgctg accgctttga tgattatgat gtaacagtca
    18961 accgccgtca tattgagctt gctagtgtta tgggtggtcg tttggctcag tacaaatcag
    19021 cggttaagtt tacaaaccaa gcaggttaaa tatagaaagg ggagtctgaa tgacaatcct
    19081 taaaacaatc aaagaaatgg tagaagttga cgttgaagag gatatctttg acgtccaact
    19141 tttacgatat atcaacagtg ggatttcata tttacaaaga aatgctattc ctgttatccg
    19201 tatcgataaa gaaacggagt tagagggatg gacagacatt aaggaagaag atagggagac
    19261 agtgcttgac tggttacatc tgagatgcgt ccaacgtttt gacaaatctt tgatgacagg
    19321 cagtgctgct actatggaat ggattgacgg agagatgaca aatcttttgt atcaactcaa
    19381 ggcaatctat gaagtgaaga catgaaatct tcaagagtag ctatcattct ttgctatgat
    19441 gagcgtgttg aggttgaaaa aggtgtctgg gagaaaaaga ccgtagaaaa gaaagtcaaa
    19501 gctaagaaag agaaaatcta tcagcgaagg cttgataaag ctatggcaga cggtcaagtt
    19561 ctgactgcta ggtttagtgt tcgttccaac tatgtgactg acactcttga ctatgtcaaa
    19621 tataatggca aagagtacaa ggttaacatt gggacagaat cagatgatgg tcattacact
    19681 atcatcgaac ttggagaact gaaataatgg ccaagaaatt tttcactaga caagatattc
    19741 aatctatcct tgaaacaaac cctttgaagg ctaaggtctt ctacatggaa cgtgaggaga
    19801 aatcttctcc tgacaatgtc atcttgtatt atcggttaac accaggcagt agcattactg
    19861 ctgatgatag agtgcacatg agaaaagtga ctttgcaagt tagtcactac cacaagaaga
    19921 aactagacag cattgaaaat ttgatgctgt ctaattttat gtgtgagcca aatcaattga
    19981 atctaaaaca acctgacact gactatctac tgacaactta cagacttgag gtgtttacaa
    20041 atgggcggtg gtagcgtgaa gatgacaccg ctaaaggtgg atatcaagaa tcaagtttta
    20101 gagaacatta agaaggctgc ccaaagtaca gaaaatgaca taagaaccaa aagccctaga
    20161 cgaaacggtg tttacgaaaa aggttggaca cacgacatca tagatgacgc cgctgttgtt
    20221 cataataatg gcaaagaaaa atctctatcc cacctattag agaatggtca tgccactaaa
    20281 aacggtggct ttgttgcccc tagagagcat atcagaccag cctacatcaa gaataaggag
    20341 aaattcctta atgatatgaa atccattaaa atcacaccta aataagaaag gagtcttaca
    20401 tgacttatca atacgataca agggaagtca ctcacggtaa tgcgaatggt ttttatgcca
    20461 aaattgctaa aactgatgct ggtgccttag acttgcagaa accttatccc tttacagggc
    20521 tacgaagcac atcctttgaa acttctcagg aatcaaatgc ctactatgca gacaatgtgg
    20581 aacatgttcg tttgcaaggt aaaaaatcca cagaagggtc tattacaacc tatcagattc
    20641 ctaaacagtt tatgattgat cacttaggta aaaagcttac gaactctaca ccaccagctt
    20701 tgattgatac tggtgtcaat actaacttca tttggggtta tgctgaaact gttacagatg
    20761 agtttggagc tgagattgaa gagttccaca tttggacgaa cgttaaggca tctgcaccta
    20821 aaggcagcac atcaactgat gaaacatcag cgacaccaaa agaaattgaa atcccttgta
    20881 ctgcctctcc aaataacttc attgtggatt ctgaaaagaa acctgtatca gaaattgtat
    20941 ggcgtgatga cagcaaagga accgtccgtg ggaagtttga caagttgttt gctgacaagt
    21001 caccaggcaa gctgattgat ttcatcaatg aagcgcttgg aacaaccact acacctacca
    21061 caactgttag cggaggctct taatgattaa aaaagaacta tcattcacag cgtttgatag
    21121 ttacggtgaa gaaagagagc acactgaaac agtgcgcttt ctttactctt taccagctat
    21181 caagatgtat gaacagcgaa ctggtcgtaa cttctttgat gataaccaaa aagcacttac
    21241 agcatataca caacttgctc ttgcaactgg tgtaaacggt aacctatctg atttaactga
    21301 tgaagagaaa atcaagatga tgcctctact tatggaacct gatttcatga acttcttgac
    21361 tgaagtcatt ccttgccttt atggtgaggt tgaaaatgga cgtttagtac aaaatgagct
    21421 taccgctgaa acagcttcat tagctccttg gtttggtgat ttgattgata ttggtttctt
    21481 ctcagacctc ttctatgaat ttaaccgaag cagagcaaag gtgccgcaag ataaaaaaaa
    21541 gccttttcag aagttgtaac ttccgaaaag gtttataagg ttatctttga aaatcggatg
    21601 gatgtttttt gggcagaatc ccaacacttt aactatttga tgggaacact tcaccaaatg
    21661 agcatcaatc aggaagagaa gaaaacctta tccaacgctg atttacttag cgtgatgtca
    21721 gattaaaaag aaaggaggaa atctatgact gaaacgtttg aaggcttata cgtcaaattt
    21781 ggtgctaata ctgttgaatt tgataggtct gtaaaaggta tcaacactgc cttatctagt
    21841 ttgaaaaaag acttcaataa catcaacaga caattgaaga tggatccaga caatgttgac
    21901 ttgttgaatc gtaagttggt taacttgcaa gaacaggctc gtgttggtgc tatgaaaatt
    21961 gctgaactca aaaagcaaca gaaggcactg ggagaatctg aagttgggtc agcacagtgg
    22021 aataagcttc aacttgaaat tgctaaggtt gaatcacaga tgaagattgt tgataaggca
    22081 atggagtcaa caaagaaaca cattgaagat gtaggagacc caaagtctat tctgaatctt
    22141 aacaaagaac ttgataatgt tgctaaagag cttgatattg tcaatcaaaa gcttgagcta
    22201 gacactgaca atgtcgaact agcagagcaa aaaatgaaac tacttggtaa acagtcggaa
    22261 ttggttgggg ataaagtcca agaattaaag aaaaaacaag ctgcccttgg cgatgagaaa
    22321 ataggtacag aagaatggcg tcaacttcaa aatgaaatcg gtcaagctga agttgaagtt
    22381 ctaaagattg accgtgcaat ggacattctt ggtgagtcaa gccgttctgc aactggagac
    22441 atcaaagagg caaccagcta tttaagagct gatgtcatga tggatgttgc agataaggct
    22501 ggtcagattg gccagaaaat ggttgacgct gggaaaatga cagtagatgc ttggtctgag
    22561 atagatgagg ctctggacac cgtcacaacc aaaactggtc tgactggtga tgccttagca
    22621 gagcttcagg aaattgctaa agacattgct actggtatgc ctaccagctt tcagaatgct
    22681 ggtgatgccg ttggggaatt gaatactcag ttcggtttga ctggggaaaa gctgaaatca
    22741 gcatctgaat tacttatcaa gtatgctgag attaacgaaa cagacatttc aagctctgcc
    22801 atttctgcaa aacaagctat tgaagcttac ggtttgacag ctgaagactt gggaatggtc
    22861 ttagacaatg tgaccaaagc cgctcaagat acaggacagt cagttgacac gattgttcaa
    22921 aaagccattg acggtgctcc tcagattaaa ggtttgggac tttcttttga agaaggtgct
    22981 gcactgatcg gtaagtttga gaaaagcggt gtggattcat ctgctgctct atcctctcta
    23041 tcgaaagctg ctgtcatcta tgctaaagac ggtaagactc tgacagatgg attgaatgag
    23101 actgttagtg ctattcaaaa ttctactagt gagacagagg ctttaagtat tgcctcagaa
    23161 atctttggta gtaaggctgc tcctagaatg gtcgatgcta ttcagcgtgg tgcttttagc
    23221 ttcgatgact tagctgaagc agctaaaagt tcctctggta ctgtctccac cacatttgat
    23281 gagacgcttg acccaataga taagttgact cagtattcta accaagcaaa agagggaatg
    23341 gcagaacttg gcggtaaatt gcttgagact gtcatcccag ctttagaacc tttgatgggt
    23401 atgcttgaat cttctgtcaa ttggtttact agcctaaacg aaactgatca acagactatc
    23461 gtgattcttg gcctagttac aactgctgtg atgatgttgc ttggtgcaat tgcaccgctg
    23521 gtcatcgcca taggggcaat aggtgcgcct gtcggaattg tagtggcggc aatagtaggg
    23581 gctattgccg tcataacact tatcatccaa gcaatcatga actggggagc cataactgaa
    23641 tggcttcagt caacgtggga ttcttgtgct gcctggcttt ctgaattgtg gactaacata
    23701 gtcacgactg ccaccacagc gtggtcaaat ttcactgcct ggctttctgg cctttggtct
    23761 tcagtagtct caactggaca gtctttgtgg tctagcttta ctagttcctt gtccaatatt
    23821 ttctcaagtt tgattacagg tgctcagtct ctgtggtcaa gtttcacttc cactctttcc
    23881 aatttgtggt ctggactggt ctcaactggg tcaaatttgt ttaataattt gagtagcacg
    23941 atttcaggaa tttttaatgg gatactttca acagcaagca atatttggaa ttccataaaa
    24001 tccactattt ccaatgcaat agatggggcg aaaaatgcag tgtccaacgg ggtcaatgcc
    24061 atcaagaatc tgtttaactt ccagattaaa tggcctcata ttccactacc tcacttccgt
    24121 gtgagtggtt ctgctaaccc tctggattgg ctaaaaggtg gcttaccaag tatcggcatt
    24181 gactggtatg ccaagggcgg tatcatgacc aaaccaaccc tatttggcat gaatggaaac
    24241 cgtgcaatgg ttggcggtga ggctggcgct gaagccatct tgccattgaa taagtcaacc
    24301 ctgggggcaa ttggtcaaag tattgctaac acgatgaata catcgaacaa tattaacgtt
    24361 aacttctctg gcgtcactat cagggaagaa gctgacctta acagactagc caacgtggtt
    24421 ggaaatcgta ttgctgaaga attgcaacgt aaaactaatt tgagaggagg aatggcatga
    24481 caaaaatcaa tgaacttacc attgacggtg tgaaaacatc atcttttaag tgtgatgtat
    24541 tggttgaaac tagaccaaat gtcattgtct ccagctccaa aacagctcta ttagagcatg
    24601 atggtattag tggtgcagtt gtgcaatcga atagacaccg tgggctaatt gagaagcctt
    24661 atcatatcac tttaattgag ccaagcgatg aagaaattta tcgcttttct gctcttttga
    24721 accgtgaaaa gttttggttg gaaaatgaac aggaaccaac tattaggctt tggtgttata
    24781 aggttgatag ctttgagatt ggaaaagacg aatttggtgc ctgggtggtc gatgttacct
    24841 tcatctgcca ccctaccaag ttttttaaaa caactgacac ccagactttg actgggaatg
    24901 gggttttgag ggtgcaaggg tcagctcttg ctttcccgaa gattacagtg gttggtcaaa
    24961 gcgcttctga gacatcgttt acggtgggga atcaagtgat taaacttgaa aagctctcag
    25021 aatcgcttgt gatgaccaac gattctgaca atcctagctt taagacggct agtggcaagc
    25081 ttattaagtg ggctggtgat tttatcacag ttgatactgc taaggggcag aatgtcggtg
    25141 ttgttttagg tgctggcata acgtccttga aatttgaaac agtttggggg tgggcatagt
    25201 tgctcttttt acttgataaa gatgttagaa cggtcaaatg gaatggcatt ccacttcacg
    25261 aggccagctc tgccattgtc aaagaagaaa ccaacagtga tttctacctg actgttcgct
    25321 atcctatcac ggattcaggt atctatcaac ttatcaaaga ggatatgctg attaaggcgc
    25381 ctacgcctgt gctgggtgct cagctgttta gaatcaagaa acctattgag aatgatgata
    25441 gcttggatat cactgcttat catgtttctg acgacatcat gaagcggtct atcactcctg
    25501 taagtgtagt tggtcaaggt tgtgctatgg cactgtctca gatggttcaa aatgctaaga
    25561 ctggtctagg tgatttttcg ttcaccagcg atattatgga cagcagaacc ttcaacacga
    25621 cagagacgaa aacactgtat tcagtcctta tggacggtaa acacagcatt gttggaacgt
    25681 gggagggtga gcttgtccgt gacaattttg ctctttctat caagcgtagc cgtggggctg
    25741 atcgtggtgt tgtcatcaca acacacaaga acctcaaatc ctatcaacga accaagaact
    25801 ctcagggtgt tgtcacaagg attcatgcac ggtcaacctt caaacctgat ggagctgaag
    25861 atgaagtgac gctcagagtg tctgttgaca gtccactaat aaactcttat ccctacatca
    25921 atgagaaaga gtatgagaac aacaacgctg aaaccgttga agaccttaga aagtgggctg
    25981 aggctaagtt tacaaatgag ggcattgata aggtttctga tgccattgag attgaagcct
    26041 atgagcttga cggtcaagtt gtaaatctgg gcgacacggt taacctcaag agtagaaagc
    26101 acagtgctga cctctacaag aaggccattg cctacgagtt taacgcttta acggaagagt
    26161 atatctccat aacttttgat gataagccag gagttggtgg ttctggggtg tcaagtggct
    26221 tgtctaatgt tgctgatgct atacttgtag caagtgccac agctcaggac gttgctgttc
    26281 aaagggctgt gaaaaatgct aacgctgcct ttgatgcaga gtttggtaaa acaaaaacaa
    26341 aaattaatga tgatatcgaa atcgcaaaag ccaaagtaga aagctttaaa tcggaactat
    26401 ctaatcgcat ggataatcag cttcttcccc ttgcgacaga agctaagaat ttagccagtc
    26461 aggcacaagc agacctgacc agaaaagaaa tagagcttcg agctgaatta aatagacaag
    26521 tcacaagcac agaaactgta aaaatctctc taacaaatct tagtcacaat atggatatca
    26581 ttaagcaaaa agccctaaac gaccttagag acgcagagac aaggctaaag gaagctgata
    26641 gcgtccaaca acttgcgaca aagcgggttg aggataaact gacaggactt agcacgaaac
    26701 tggagtcatt ctctgttggt ggctataatt atgtcattga cggcggagaa cctaaagaac
    26761 taatggctaa cttctatggt aagacctatg atattaatcc ccaactactc gaacgaacta
    26821 gtcaagcgac cttgagtttt agctatgaag cagaatcaac tagtcgttta gaagttagac
    26881 tttataaaaa gatgcacacc ggggacacta gtaagattac aattattgtc atgcctaact
    26941 ttgacttatc tccagggaaa ggatttatct ctcagagctt tgatttaggt ggtgttatgc
    27001 ctgaccctag aaaccaagcc tggcttgtga tgcgggggac aaatgctaac cccttgacgc
    27061 tctcgaaagt taagctggaa cgtggtaccg ttgctacaga ctggaataat cgcgatgaaa
    27121 ccttaaaagc tagcttcgcc gaatacaagc agaccgttga tgaaaactta gccaacctta
    27181 gaacaagtac ggaaaccttg gcaggtcaac tgacaagtgc tgagtctagc attaggcaga
    27241 cgtcggaatc ctttagcaat cgtttggtta gtcttgagac ctataaagat agtgaatcaa
    27301 atcgagctag tcgttacttt gaagcaagca agtctgaaac cgctaagcag ctatcagcct
    27361 tacgaacaga agttaatggc tcttatgtgg ataagtcaac ctatgaggaa aatgcaagag
    27421 gtttgacaag acgctttgaa agtctgtcat cagggacaca gaactatgct ttggggactg
    27481 aaagagatgt ctatctaaac gtttcaggga ctaccgtcta tgacctttat agcttcggca
    27541 ctaccttagc aataaaaggg attgaaatgg gagatactgt aactattgca tttaactatt
    27601 ttgcggatag tcggacggct tttggttctt atgaactgga gctatacgga catgcaggca
    27661 aaatcgaacg ggttggtgag gtacagagaa cgatatcaaa tcgtggtacc tataccgcat
    27721 cttttgtggc aaacaatgcc aatttcaggg caaactccct taaaattaga tttacggata
    27781 gccgacttaa gtttagagtg acaacacttc gtgtgactaa ggggacaatt cctgcggact
    27841 ggagtccatc tccagatgac ctaaaagcct actcggatac taagcttgaa cagacagcta
    27901 atgagattaa agctagtgta acaagtcttg atcataaaac tcttaagcaa actgatatta
    27961 ctatgacttc tgaagggatt gtcctaagag caggcaagac gagtaatgat gtggcaagag
    28021 ctattggctc ttactttaag gtcactcctg atgctatagc tctgtttagc tcactgataa
    28081 aagttagcgg gaacatgttg gttgatggtt ctgtaactag tcgtaatttg gtaacaggcg
    28141 ctgtcgaaac gggacatgtt aaggctggag caatcacagg agtgctttta gcagctgaag
    28201 ctgtaacggc agaaaaactt aaagtcgacc aagctttctt taacaagttg atggccaacg
    28261 atgcctactt gaagcaacta tttgccaagt cagccttcat cactcaggtt caatccgtga
    28321 caatatctgc cagtcagatt tcgggcggtg ttattaaagc tttgaataat gccatggaaa
    28381 tccagatgaa cagtggtcag atactttact acactgacca agctgcactg aaacgggttt
    28441 tgagtggcta tcctactcag tttgttaagt ttgcaactgg tacagtatct ggtaaaggaa
    28501 atgctggtgt tacggtcatt ggttctaacc gctacggtac agaatcaacc aatgatggtg
    28561 gatttgttgg agtccgtgca tggaacggct caaacattga ctcacttgac ttagttggtg
    28621 ataaaatccg attagcaagc tctgcctttg acaattcaga tggttgggat gttcggacac
    28681 ttgattcagg cttgaaaatc acaccacaca atagggctgc tgaacgcaat agccgaattg
    28741 aggttggtga tgtctggatt ttaaaaggaa atggatccta ttcttctctc agagatattt
    28801 tgaactcatt caatgagaac ttctcaaaag gcccaaatgc tgactcttac acttactacc
    28861 attcggggtt ctaactagaa aggaacaatc atgaaccaag aacaacaact caatcaagcg
    28921 ctacgcttga ccgtcaatga attgacagcc cagttggcca atgagtcaac cactaagaac
    28981 ttgctggcta tccagctgac tgaagttgat caggagaaac agcaactgac tcagcaaaat
    29041 gctgaacttc aagcaagagt ttccgaacta gaaggcttgc ttgatgaaca aactcaacca
    29101 gaaatcattg aaggagaata accatgacta caacagaaaa taatttactt gaccttgaaa
    29161 caatcacaga accctttgac ttggcgacag cactcaagta catgaaggaa aatggtgagt
    29221 taatccgttg caagaatgct gtcaatgact tctacatgta ccgtgacatg cagaaacgtc
    29281 ctgtcatcgt caatggtcgc cgtcaattca aggacgtgga aactgtctgg gcatttaacc
    29341 aatggggcgg gacaacaccc acaatcaaca tggctgatct ctttaatctt gaatactaca
    29401 tcatgacctt tgatgagaac ggaaatcctg actggactga gcctcacttg gaggataagt
    29461 aagctcatct aaaataaaca ggataggaga tagtgaatgc caccatggtt aatagatagc
    29521 actgtggttg tggcgatggt gacggtgctg ggcggtctgt tcagcactat tatcaccact
    29581 tcagccaatc ggaaagacca gctgataaaa catcagtatg aggatatcaa ggaagatttg
    29641 tctggcttga ttgacaaggt taagacgatt gatcatacaa ccacggaaac aaagaaaatt
    29701 tcagaaatca caaaagacgg tacgcttaaa atccagcgtt accgtctttt tcacgatttg
    29761 acaaaggaaa tttctcaagg ctacacaact attgaacatt tcagggagtt gagcattctc
    29821 tttgagagtt atcaactgct tggcggaaat ggggaaattg aagccttgtt tgagaaattc
    29881 aaacagttac caattgagga ggactaaaca tgacacaaat tacagatatt atcatcagct
    29941 cagctatggg aattttgact attttggcag gaattgcagt gcaagctgtc aaggtttatt
    30001 tgattaaaaa aggcggtgaa aaggccgtct tgattacaga aatcttggcc aagaacgctg
    30061 ttaatgctgt ggagcaagtg gccactgaaa ctggcttcaa aggagctgac aagctaactt
    30121 ctgctaaggc tcagatttta gctgagttgc aaaaatacaa tatccacatg tcagacgatg
    30181 atctcacttt gtttgttgaa tcatctgtca aacaaatgca cgatgcctgg aaggagtaac
    30241 ctatggaaat caacacagaa acagccattg cctggatgtc tgcacgtcaa ggcaaggtca
    30301 gctattccat ggactaccgt gacggaccta acagctatga ctgttccagc tctgtctatt
    30361 atgctctcag gtcagctggt gcaagctcag caggttgggc ggtcaacact gagtacatgc
    30421 acgattggct gattaaaaac ggttatgagc ttattgctga aaacgtggat tggaatgctg
    30481 tccgtggtga tatagcaatt tggggcatgc gagggcactc aagcggagct ggtggtcatg
    30541 tagtcatgtt tattgaccct gaaaatatca ttcactgtaa ctgggcaaat aatggcatca
    30601 cagtgaacaa ctacaatcag acagcggctg ctagtggctg gatgtattgc tacgtttacc
    30661 gtttgaaaag cggagcttct acccaaggaa aaagccttga taccttggtc aaggaaaccc
    30721 ttgctggtaa ctacggtaat ggcgaagcac gcaaggcagt gcttggcaat caatatgagg
    30781 ctgttatggc agtcatcaat ggcaaaacta cgactaatca aaagactgtt gaccaacttg
    30841 ctcaagaggt aatcgctggc aaacatggca acggtgaagc tcgtaaaaag tcgcttggta
    30901 gtcaatatga tgcagttcag aaacgagtga cggaattgct caaaaaacag ccctctgagc
    30961 cgtctaaggc tcaagaggta aacaagccca cggaaaccaa aacaagccaa acagagctaa
    31021 ctggacaagc cacagccacc aaagaagagg gcgacctctc tttcaatggg actatcttga
    31081 aaaaagcggt gctggacaag attctgggca actgtaaaaa gcatgatatt cttccaagtt
    31141 atgcactgac tatcctacac tatgaaggtc tttggggaac ttcagcagtt gggaaggcag
    31201 ataataactg gggcggtatg acctggactg gtcaaggcaa ccgtccaagc ggtgtcactg
    31261 tcacacaagg ttccgcacgt ccttcaaatg agggcggtca ctatatgcac tatgcaagcg
    31321 tggatgactt tctgacagac tggttttatc ttctaagggc tggtggctct tacaaggtgt
    31381 ctggtgctaa aaccttcagt gaggctatca agggaatgtt taaagttggc ggtgctgtct
    31441 atgattatgc tgcaagcggc tttgatagct atattgtcgg agcttcaagc cgcctcaagg
    31501 ctattgaggc agaaaatggt tcactggaca agtttgataa aactaccgac attggtgacg
    31561 gtagcaaaga caagattgac attactattg aaggtattga agttaccatc aacggtatca
    31621 cttatgaact gactaaaaag ccagtttaga attagatacg ataagacaaa ataagccctc
    31681 agcaattgcc gggggctgtt tttccgttat aacggacttt cctcaaaata tcggttataa
    31741 tggacttttt ttgataaaaa ttttaaaaat atttgttaaa aacaagtgtt ttctattgac
    31801 aagtgtttaa aacaagtgta taatacaatt aaagataagg aaaggagata agccaatgac
    31861 agagcgagag cttaagaaaa ttgctaagaa gcaaggtttt agtaaaacta actttggcaa
    31921 agggtcacac gaagtttgga aacatccaga cggacggaca gtgacgatac ctaagccaaa
    31981 ggaaaaagat tacagaccag gcacactgaa caacattctc aaagtcttgt atggggagtg
    32041 aggacgctcc tccccgtacc cctaaaaggg gatactctga tcattggctt aatctaatat
    32101 tatgaaatac acttatttag cattgtttga agtggataaa gaaaacggtg gctataacat
    32161 ttctttccct gattttcctg gagcatttag tgaagctgat agcttgaacg aggccatttt
    32221 taacgctcgt gaagtccttg aaatctatac aattatgttt gaagacgagg gcaaagagtt
    32281 tcctaaagca tcatcattca aggctcttgc aagcaattta gcaagtgacg aagatgtgat
    32341 tcaggctatt tcagttgata ctgagcttgt ccgtgagcgt gaacgctcta agattgtcaa
    32401 caagactgtc acactgccaa gctggcttgt ggaagttgga aaagaaaaca aggtcaactt
    32461 tagccagctg ttgcaaaaag caatccgtga ggaattgcaa gtataagaaa gagcggtcaa
    32521 gatggccgct tttttctgta taaaaaatta tgaaaataga tagtaatact tttcaaaaat
    32581 tgacctatga aaaaccgtcc gccccaaatt cgccccaaat tatttttaaa gttagcctga
    32641 tttaacctga tggaaaatca aaaaagccta taaaatcagg cttttcattc ggttaattcc
    32701 tgttaattca ggtactgaaa ggcggtagac ggatttgaac cgacgatcaa gcttttgcag
    32761 agccgtgcct taccacttgg ctataccgcc ataacagata atattctatc gaaaaaaaaa
    32821 agattcgtca agtctctttc gtaaaatagc caattttagc ccttaaactt cctcatatta
    32881 ccaaaaaaag agattgatac aatgggtatc aatctctttt agattattgt ctgattaaat
    32941 aatcaaatgc gccaatggca gctgtggcac cagaccccat ggaaataatg atttgcttgt
    33001 aggcagcatc tgtacagtca cctgcagcaa aaataccagg gatatttgtt gagccaaatt
    33061 tatcaacgac aatttcttga cgttcattga gttcaatgcc gctatccttg agccaagaag
    33121 tacttggtac aagaccaatt tgtacaaaaa caccttctaa atcaatgtgt ttttcttcgt
    33181 ttgtatcacg atcggtatag tttaaaccag tgacatgatc ttcaccaacg atatctttag
    33241 tagcaacatt cttaagaata gttaaattgt cagttttagc tgctcgttct tgcagtactt
    33301 ggtctgcttt gagctcaggt aagaattcta ggactgtaac atgtttagtg acaccggcta
    33361 gatctaaagc agcttccata cctgaattac caccacctat aacagcaaca ttttttcctt
    33421 cgaaaagagg gccatcacaa tgtggacagt aagtaacacc tttattgcgg aattcttcct
    33481 cgccaggaac gttgatattt cgccacttag cacccagagc taggatagca gttttagctt
    33541 gtaaaatagc tccgttagcc agtgttacct caactaattc ttttttctca atgccagttg
    33601 ctaattgact cttgataata tcaatatcgt aagatttggt atgctcttca atttgagcca
    33661 ttaatttagg gccttcggtg tacagtgtac caatcatgtt ctcaatacca actgtttcga
    33721 taacttgtcc accaaaagtt tctgctaaaa tacctgtttt taacccttta cgagcggcat
    33781 aaattgcagc actgttacct gcaggaccac caccaataac tagaacatca tagacacctt
    33841 tgtcagcaaa tgcttcagca tcaagaggtc catctagttg ttctaaaagc tgttcaattg
    33901 tagcacggcc agaagtaaat tcttcttggt ccttataaac agtaggaaca gacatgatac
    33961 cttttgattt gacttcgtct tgatacatgc ctccttcaat catagtatga gtgatatttg
    34021 gattcaaaac agccattata ttaaaggctt gaacgacatc tggacaatta tggcaagtta
    34081 atgaaacata agtttctaga ttgatagttt tctcaatccc cttaattcgt ttgataatat
    34141 cttcatcaac ttttggtgcg cgaccagaga cttgcaacaa agctaaaatg aaagatgtga
    34201 attcatgccc cattggcaag cctgaaaaaa taacgcgact ttcttgccct ttttttgcaa
    34261 tcccaaaact aggttgacgt tttaaatgtg ttgattcaag gctgatacga tcagacatag
    34321 cgacaatttc atctagaaaa tcctttactt tttgagaatt atcgttatct cctaaatcag
    34381 tttgtaatac aatatcagat tctagcaaat ctaaatattg cgctaattga gccttgattt
    34441 ctttatctaa gaccatggat tacctcctgg ttattaaatt ttacctacaa ggtctaaact
    34501 aggagttaaa gtttcagctc cttctttcca tttagctgga caaacttcac ctggatgttg
    34561 gcggatgtat tgagcagcac ggactttatc aatgagagta cttgcatcac gtccgatgcc
    34621 atcagcatta atttccatca tttggatgac accgtctgga tcaatgataa atgtaccacg
    34681 ttgtgctaga ccatcttgtc caaggacatc aaaaccttgt gaaataaggt gtgaagggtc
    34741 accaatcata ggatatgtga tagtaccaac gacatctgag tcatcatgcc atgctttatg
    34801 aacaaaatga gtatcagtag agacagaata aacttctaca tcaagagatt taagtgtttc
    34861 gtattgctct tgaaggtcac caagttcagt tgggcaaaca aatgaaaagt ctgctgggta
    34921 gaaacaaaaa actgcccatt ttcctttaac gtcttcattt gtaacagtga tgaattttcc
    34981 atcgtgataa gcttgagctg aaaattcaat aatttctttt ccgactaatg acataataat
    35041 gtcctccttt attttttaat agcctaatta taaacgaaat acttttaaat ctccaataaa
    35101 aacgtttaca tttttctaag aaaagtatga tttttctaat agagtggaag attttatagc
    35161 ttgtcaaatc taactttttt tgataaacta ctatagttgc ttaaagtgac aaatactcat
    35221 cttagatcaa ttgtggtctt gttgccgaaa ggttaggctg caagtcgaag tctgagagag
    35281 gaaaaaacat ttttaaagga gaacttactc atggcagtaa tttcaatgaa acaacttctt
    35341 gaggctggtg ttcactttgg tcaccaaact cgtcgctgga accctaagat ggctaagtac
    35401 atcttcacag aacgtaatgg tatccacgtt atcgaccttc aacaaactgt aaaattagct
    35461 gaccaagctt acgaatttgt tcgtgatgct gcagcaaacg atgcagttat cttgtttgtt
    35521 ggtactaaga aacaagcagc tgaggctgtt gctgaagaag ctaaacgtgc tggtcaatac
    35581 ttcattaacc accgttggtt aggtggaact cttactaact ggggaactat ccaaaaacgt
    35641 attgctcgtt tgaaagaaat caaacgtatg gaagaagaag gaacttttga acttcttcct
    35701 aagaaagaag ttgcattgtt aaacaaacaa cgtgcacgtc ttgaaaaatt cttgggtggt
    35761 atcgaagata tgccacgcat cccagatgta atgtatgttg ttgacccaca taaagagcaa
    35821 atcgctgtta aagaagctaa aaaacttggt attccagttg tagctatggt tgacactaac
    35881 gctgatccag atgatatcga tgttatcatc ccagcaaacg atgacgctat tcgcgccgtt
    35941 aaattaatta cttctaaatt agctgatgca gttattgaag gtcgtcaagg tgaagatgct
    36001 gacgttgact tcgctcaaga agcacaagca gattcaattg aagaaatcgt tgaagtagtt
    36061 gaaggatcaa acaacgacta atatttaatt tattgtaaat cctaaaacgg ggcagatgca
    36121 aaccgctctg tctcgttttt ttaatcatta tttgaaatta agaggagaac gaaaatggca
    36181 gaaatcacag ctaaacttgt aaaagaattg cgtgaaaaat caggtgcagg cgttatggac
    36241 gctaaaaaag cattagtaga aactgatggt gaccttgata aagcgattga attacttcgc
    36301 gaaaaaggta tggctaaagc agctaaaaaa gcagaccgtg ttgctgctga aggtttaaca
    36361 ggtgtttatg ttgatggtaa cgttgcagca gttattgaag ttaacgctga gactgacttt
    36421 gttgctaaaa acgaccaatt tgtaacatta gttaatgaaa ctgctaaagt tattgcagaa
    36481 ggacgtcctt caaacaatga agaagcactt gctttaacaa tgccttcagg tgaaacttta
    36541 gaacaagcat ttgtaactgc aactgctaca attggtgaaa aaatttcatt ccgtcgcttc
    36601 gctttagttg aaaaaactga tgagcaacat tttggtgcat accaacataa tggtggacgt
    36661 atcggagtaa ttactgttgt tgaaggtggc gacgatgcac ttgctaaaca agtatcaatg
    36721 cacgttgcag ctatgaagcc aactgttctt tcatatactg aacttgatgc acaatttgtt
    36781 catgatgaat tagcacagtt gaaccacaaa attgagcaag acaacgagag tcgtgctatg
    36841 gttaacaaac cagctcttcc attccttaaa tatggatcaa aagctcaatt gactgatgaa
    36901 gttattgccc aagcagaaga agacatcaaa gcagaattag ctgcagaagg taaaccagaa
    36961 aaaatctggg ataaaatcgt tccaggtaaa atggatcgtt tcatgcttga caacactaaa
    37021 gttgaccaag agtacacact ccttgctcaa gtttacatca tggatgatag caaaacagtt
    37081 gaagcttatc ttgagtcagt aaatgctaaa gcagttgctt tcgtacgttt tgaagttgga
    37141 gaaggtattg agaaagcttc taacgacttt gaagcagaag ttgcagctac aatggcagca
    37201 gcacttgaaa aataattaac actcgcttta tattataatt tggcagcaaa atgttgtcag
    37261 taaatatatt ttaaaataag acacttggct taagtcaagt gtcttatttt gctatcgaat
    37321 tggtggattg gcaaatatta aaaaggagag acagtcatct caccttaact tttctagaat
    37381 aggttgaagc cgataatgga taatgttccc atgatgcatc cagctagtat aacacctagc
    37441 ataacagcaa taacactatg tttaaattcc caatctagga ggctagctgc aagtgtgcca
    37501 gtccatgctc ctgttccggg taagggaatg cctacaaaca atagcaaggc aataaacaat
    37561 cctttttctc cagctacttt ttcaagtttt tgaccaccgc tatgtccttt tttaaggcac
    37621 catgtgaaaa acttaccagt ataaggttta tctgctcccc attctagaac tttgcgagca
    37681 aagaagaaga tgataggtac aggtagcatg tttccaacaa caccgatagc tagcgcttcc
    37741 cacaaaggta ttccattagc gattgcaaat ggcacagctc ctcgtagttc cactaaggga
    37801 atcattgaaa tcaaaaatgt aataatgtaa ttcattaaat aaaacctttc tatggtatta
    37861 ttccatttta tcttatcttg ctagtaatga caactaaaat ccgcggtcat tctagtttta
    37921 ctatccttag atagatttca tttccgtatc ttttaaaaat ttgatataat aatagtcagt
    37981 tatagtcaaa gaggaaggag tgaatttgtg gccataaaaa atacttccga taatattgaa
    38041 gaatatataa aatcattatt agaacaatca ggaattgctg aaataaagcg ttccaattta
    38101 gctgatactt ttcaagttgt tcctagtcag attaattatg tcattaagac acggtttact
    38161 gaaagccgtg gttatgtggt tgagagtaaa cgaggtggtg gaggttacat ccgcattgct
    38221 aaggttcatt tttctgacca acaccaatta tttggaaata tgttatcaac gattggcgag
    38281 cgtatcagtg aacaagtctt tgatgatttg attcaattat tattcgatga agagataatt
    38341 acagaacgtg aaggaaattt gattctggcg acatcaggtg atgacgtctt aggagagcaa
    38401 gcctctgtta ttagagctcg tatgctccgt aaattattac aacgattaga tagaaaagga
    38461 ttccatttat gagtcattat tccataaaat tacaagaagt attcagattg gcgcaatttc
    38521 aagcagcacg ctatgaaagt cactatttag aaagctggca tcttctttta gcaatggttt
    38581 tagttcatga ttcagtagct ggtttgacat ttgctgaata tgagtctgaa gttgcaattg
    38641 aagagtacga agcagcaaca attttagctt taggtagagc accgaaagaa gagattacca
    38701 attatcaatt tttagagcaa tcaccagctt tgaaaaagat cttaaagtta gcagaaaata
    38761 ttagtatcgt agtaggagct gaagatgtgg gtactgaaca tgtcttgctt gctatgttgg
    38821 ttaataaaga tttattagcc acacgtatct tagaattagt tggctttaga ggtcaagacg
    38881 atggtgaatc agttcgtatg gttgatttac gtaaggctct agaacgtcat gccggtttta
    38941 caaaagatga tatcaaagca atttatgaat tacgtaatcc taaaaaagca aagtcgggtg
    39001 cttctttttc tgatatgatg aaaccaccaa gtactgctgg tgatttagct gatttcacac
    39061 gtgatttatc acagatggcg gttgatgggg aaattgaacc tgttattggg cgtgataaag
    39121 aaatttcacg tatggtccaa gtcttaagtc gtaaaacaaa gaataatcct gtcttagttg
    39181 gagatgcggg tgtcggtaaa acagctttgg cgtacggtct agcacaacgt attgctaatg
    39241 gaaatattcc ttatgagctt agagatatgc gtgttctaga gttagatatg atgagcgttg
    39301 ttgctggaac tcgttttcgt ggtgattttg aggaacgtat gaatcaaatc attgcagata
    39361 ttgaagaaga cggtcatatt attcttttca ttgatgaact gcatactatt atgggttctg
    39421 gctctggtat tgatagcaca cttgatgctg ctaatatttt aaaaccagct ttggctcgag
    39481 gcactttacg cactattgga gcaacaactc aagaggaata tcaaaagcat attgaaaaag
    39541 atgctgcttt atctcgtcgc tttgcaaaag ttttagtaga agaaccaaat cttgaagacg
    39601 catatgaaat tctacttgga ttgaagccag cgtatgaagc ctttcataat gttaccattt
    39661 cagatgaagc agttatgact gctgtaaaag tggctcatcg ttatttgaca agtaagaatt
    39721 taccagactc agctatagac ttacttgatg aagcttctgc cactgtgcaa atgatgatta
    39781 agaaaaatgc tccatcgctt ttaacagaag ttgaccaagc tattttagat gatgatatga
    39841 aatctgcttc taaagctcta aaagcaagtc ataaggataa aaagcataag cctattgccg
    39901 taaccgaaga tcatattatg gcaactctca gccgtctatc tggtattcct gttgaaaaat
    39961 tgacacaagc tgatagtaaa aaatacctca atttagaaaa agaattgcat aaacgtgtta
    40021 tcggtcaaga tgatgcagtt actgccatta gtcgtgctat ccgtcgcaat caatctggca
    40081 ttcgtactgg taagcgtcct attggttcct ttatgttctt gggaccaact ggtgttggta
    40141 agacagagtt agcaaaagcc ttggcagaag tgttgtttga tgatgaatca gccctaattc
    40201 gttttgatat gtcggaatat atggaaaaat tcgcagcttc tcgcctgaat ggggcacctc
    40261 caggttatgt tggttatgat gaaggaggag agctgactga aaaagtacgc aataaaccat
    40321 attcagtttt actttttgat gaagttgaaa aagctcatcc agatattttt aatgtattat
    40381 tacaagtgct tgatgatggc gttttaacag atagtcgagg acgaaaagtt gacttttcaa
    40441 atactataat cattatgact tctaatttag gagcaactgc attacgtgac gataaaacag
    40501 ttggttttgg agctaaggat atcagccatg attatactgc tatgcaaaaa cgtattatgg
    40561 aggagcttaa aaaagcttat cgaccagagt ttatcaatcg tattgatgag aaagttgtat
    40621 tccatagtct cagtcaagat aatatgcgtg aagttgtgaa aattatggtt aaacctttaa
    40681 ttttagcgct taaagataaa ggtatggatc tgaaattcca gccttcagcc ttgaaacact
    40741 tggcagagga tggctatgat atagaaatgg gtgctcgtcc actgcgtaga actattcaaa
    40801 cgcaagtaga ggatcattta tcagaactac tattggcaaa tcaagtgaaa gaggggcaag
    40861 taattaagat tggcgtatcc aaaggcaaat tgaaatttga tattgcaaaa tcataaaaca
    40921 ttccagtacc gatgggtact ggaattttaa tataaaaaga aaatgtccaa aatattttgg
    40981 acattttctt atagatttat gaaaagtaaa aggatctata tctaacaatt gcacttgtag
    41041 atataggtac gactgaaagt cgttgaaaaa ttttaggata aatatattat aatatcctaa
    41101 tcttttgcta acttaaaata aatctaaaga ttaccttaag atttcaatcg cttttgtaac
    41161 catacaatgt catgccattg ttcaaattta aacccgattt ttgggaaatg tgcgacttga
    41221 ttaaatcctc tttttttgtg gaatgcaatg cttatatcat ttggtaaact gatacaagcg
    41281 agtagattaa catacccttg gttaactaat tctttctcca atgtatcata tagttggcta
    41341 ccaatttttt tgctacgtac atctttatca aggtaaatag aaagctcgca agtccagtca
    41401 taggaagctc tagcgtagta agttgaagca tatgcataac ctaatatttt cccattttct
    41461 tcagcaacta aatatgggaa agatttgctg atatttttga tacgattagt gaattccgat
    41521 atactaggaa tatcgtattc aaacgttatt gtagtgtcag taacgtaggg agcatagata
    41581 gcaagtaatt ctttagcatc tgagggtagg acaggtctta taatcataga tatcacctaa
    41641 ttatagtaaa tcaagttttt gcaattcgga atcaatagtg tcaaggacgt gttgaagatc
    41701 ctctggattt ttgacgaaat caagtttgtt accatcgatc cgaattttag gtgaaacgtc
    41761 gtaattttca taccattctg gatattcaga gtggacttgt ttgtagtagt cgtataattc
    41821 tgggttagaa tctacttgct caaagctgcg tcctcgtttg tcaatacgtt ctaacatttt
    41881 atcaaatgaa acatcaatgt agactagcaa atcaggtctc ttcttaggca taccttctaa
    41941 ttcttccaac atatttgcaa gaagttcttt atagatatct agctctgttt tagtcacatt
    42001 tccattctta aaatttagag tgaggaatag ttcatcttca aaaatagaac gatccaaaac
    42061 attgttgtta gccttatagg cttcttttat agattggaat cgtttattta aaaagaatat
    42121 ttgtagtaaa aaagcatatt ttttgggatc ttgatagtaa agatctaaaa ctgggttatt
    42181 atcaaccgcc tcatagaaaa catctgtccc taggtgttgt cctagagcag ctgcaaggga
    42241 gcttttacct gctccaatgg ttccagctaa aacaatcaac ataattctcc ttaattctgt
    42301 tatactaaaa taatttgtta tacttttgag ttttcattac acaatgattt atgctaaatt
    42361 tgtacagaga taggaaaaaa cagtcaaagg actgtttttg atttagcgta aacctgcaaa
    42421 aagttcttga acttctgcta gtgtttctgc acgtgaaaca gctcctctaa tttttgctgc
    42481 gcctgatttt ccacgtagat aatgaggtgc taaccctcga aattcacgaa cggcaatagt
    42541 ttcacctttt aaattaacta gtcgagtgag atgatcctcg gcaacatcta acatcttctc
    42601 aaatggtagg tcgggaagga tttcgcctgt ttcaaagaaa tgattaattt gtgtaaagat
    42661 gtaaggattg ctacgagcac cacgtccaac cattatagcg tcagctccaa tctcttcaat
    42721 cataaatttg gcatcgtgaa cagtacgaat gtcaccattg gcaataaaag gaatactagt
    42781 aacagcttta gcgaccttgc caagtgtttc atggtcacac gtgccagtat acatttgttc
    42841 acgtgttcga ccgtgcatag caagtgcaga tacaccagca gattcggcag cgagtgcatt
    42901 ttctacagca ttagatgagt cagaccatcc tgtacgcatt tttactgtta atggaatgtc
    42961 aagtacagac gttacctctt taacaatatg ataaattttt tcagggtcac gtaaccattt
    43021 agctccagct tcgtttttaa caactttgtt aactggacat cccatattga tgtctacgat
    43081 atctgctttc gtattatttt ggataaagtc agctgcacgt tttaagcctt ctgcatcgcc
    43141 accaaatagt tgaattgaca taggatgttc attttcatcg atatggagca tatgtaaggt
    43201 cttttcgttg ttataaagta accctttctc agaaatcatt tccataacaa cgagaccagc
    43261 accaaattct ttagcgatag tacgaaaagc agaattagta atacctgcca tcggtgctag
    43321 tacggtacga tgaggaattt caaccttccc aatcataaat gaactattta attgagtcat
    43381 taattatctt ctccaaatct tcttctgtga aatagtaagt ctggttgcaa aattgacaag
    43441 taatttcaac acctttatct tcatccttca tggcttgcaa ttctttagtt cctaaagtca
    43501 atagagcggc ttcaaaacgt tcttttgaac agtcacaata gaaggctaaa gagtcttccg
    43561 ataagcgctt gtagtcgtct tctccgtaaa tagctgaaag gagagattct atatgatttt
    43621 cagactctag cagacttgaa atagaaggca tctcctgaat gcgtttttca tagcgagaaa
    43681 tttcttcgtc agaagcacct ggtagcacct gaagcatgaa tccacctgca actttaacct
    43741 tatcctcgtc atctaagagg acatttaaac cgacggcaga aggtgtttgc tcactttcgg
    43801 ttaaataata agcaaaatct tctccaattt ctcctgtaat tagtggagtt gttgaagtgt
    43861 aaggttgccc agttccgtaa tcggtaataa cgacaaagtg tccattcccc atgaaaggtc
    43921 caactaagac ctctccagta gctgttttct tgatatcaac acccgtattt tggatgtaac
    43981 ctttaacatt tcctttagta tcagctacag aaataatatg accaaaagag ctatctccaa
    44041 taaccttgac agttacttta ctatttcctt tttgatttgc tgctaaaatt tgatttgcaa
    44101 ttaatgttct cccaagagct acagtagaac ttgaaagtgt ctgatgtttt tcttgagctg
    44161 ttcttacagt ttctgtacaa tcaaggacat aggcccggaa agaaccggaa gttgaaattg
    44221 attttataat tttatccata agtcatatta tagcataaaa ggcttatttt gcattggaga
    44281 tagtcaggaa aatatagatg aaaacggtat aaattaatga tataaaaacg atactgtgtt
    44341 ttacagtatc gtttttatat agtggatatg atagtaagtt aagactcatc aactttttct
    44401 tgggtagatt taccttcgtt gagtaaggct tcaactattt ttttatcacg aattttgaca
    44461 gaatcattga ttgtttcagc cgatttaata acagcagatt ctaattgggc acgttcctta
    44521 cgtcctttgt ctatggcagc gataatacca ttattttgag ccactaagct ttcagctaat
    44581 gcagtgacag atttaataga aacagtgggg ctttgtgcgg tcttctctaa catcggaatc
    44641 gcttctttac tagtttcagc cagcatttgc aatgctgcat tattagcgtt gacaatagca
    44701 tcagcagtga caccggattt gacagattgt tgcatcatgc ctaactgagc gattgagagt
    44761 ttcattgttg gaatggtatt tcgacgtaac ataccaagtt tctgacgcat atctgacgat
    44821 actttgacca agtttcgcat ctgtggtgtt gttgcccatg caacgtagag acggctgaca
    44881 tattccgtat gttgctgttc gagggtattg ataacttcag tcattcgggc taattggtta
    44941 cttttaattt gatattcgga cgtttggcta tctaatgcta gaatttcttg ttgtaagtgg
    45001 cttgcacgat tggcagcctc ggcttgactc gattcaataa aagcaataac tccaaccaaa
    45061 ttttcaatag atttagtatt atcttcaatg agcatttcag cagagacgat atttcttgcc
    45121 aaagtatctt cttgtttgac aacatttgct gccatcatat ccattttttg ctcgatgttt
    45181 tgtgagtcaa aataaaattc ctgtagcgag gtcttgcttt gtttgaataa tttttgaatc
    45241 aagtttggtt ttttctctaa ttctgccgga gtagcatctt tatatttggc aataaatcca
    45301 tttagttcgc gattagcatt ttttagtaaa tcatcaactt gaggaatttg aatttttttc
    45361 tgctcagaca agatatgatt aacagtagta ttaacgcctt ctactgcgga ttgtccaaaa
    45421 tcaaggagcg cattttggtc accgacaaaa gtatctacca aagctggtgt tttttcagag
    45481 atagcagact tttgtgctgg tgttagtttt tcaaaaaagg caatttgccc agtttggctt
    45541 gttgtctggt tggaaataat ttctgttgtt ttatctgttt tagtgatagc attgtctgca
    45601 atttggtcaa tatcaaaatt aaaggtatcg ctcatttatt gttcctttcg tcttgcatca
    45661 ttcttaggct aacgtcaaaa tctttcatat cactttcatt caattggcgc agcgtttcat
    45721 ctaaatctaa attaaatttt ttaattgctt gtaatgcttg ttctaggcgt tccttagcat
    45781 tgttaaaatt tttaggagat tgtttgattc tgatgtagcc agccagaaca tctgtaaaac
    45841 gtttcatatt ggcttgatga attgctgcta attcagcttt gttatctgcc tcttcaatct
    45901 tttttaagat agcatagtga tcatcttgaa tattgtgata gaacggtttt aattccggag
    45961 caatgcgtat catttcatct tcctgatcag aaataggttc tagttctgga gaggcatgta
    46021 gctttattaa ttgttcgtta atctcattac taaatgtatt tgcttttttg gtgatacgtt
    46081 tatagatatt aggctcaatg gctttttgta gttggtttgc ttcattacga attaggtcca
    46141 attgaggtaa tatttgatgt gctaatttga tatattcctt agaacgattg tcttttttta
    46201 aaatttccaa gtgtctaatg tcttggtcag tctctttaat atcggatttt aaagcttcaa
    46261 tacgttggtt tgatgtaaaa catttaagac gctttttatc ataaacgtaa taggcacaat
    46321 aaccaccacc aataattaac agagtagcaa tcacctcact ccaaaagtct gaaatcaggg
    46381 aagcaagaac aacaatacca agtaatgtta ttagattatg agaatttttg gaatagcgtt
    46441 ttttaaccat aataatattt aacctaactt tctgtagttg ttaatatttt atcttaaatg
    46501 aactagtgat gacaagggat aactttattt taaccaaaaa ttccttaagc ttagcctaag
    46561 gaactctttt tagttataat tctgggaaca gtttagccaa aattcgaggt gtaatagatt
    46621 gaccaggccc ctcttgacag taggtgtgca tatacatcaa tgggttaaag tttagttcaa
    46681 tgcaagtagc atttttctta tcttttgagt atgcttgagt ggcattagga ataattaaat
    46741 caactccaca aacccaagcc cccatagcct ctgccatctc agctgctagt tgtttatatg
    46801 taggatccat tgtatttgtt acatctatag agtcaccgcc agtggaaata ttagaattac
    46861 ggcgaagttc tatttttgtt ccttctggag ggatactgtt aacagtgtat ccttgttgtt
    46921 ccaacattaa ctgttctact tctccgagct caataacctc tagaggagag cgatgatcat
    46981 agcctctgag agggttttgg tttttaagtt taactagctg actaatagta tgaataccat
    47041 ctccaacgac attagctgct accctaagca atacagcaat acagtccccc tcaaggacga
    47101 agaaacggta ttcggtaccc tcaatatatt cttccacaag aatagcgcta tcttcggcaa
    47161 aagcaatgtc aattgctttt tcataagaag ctaagttagc tgatgtttta aaaatagaaa
    47221 tgcctaaacc aaagtttgta gattttggct taacgacgat tggtttatct tggatttgtg
    47281 aaaaatagtt aagtgcctct ttacggtcag taaattcatc tccaaaagga gttgggaaat
    47341 gcttttcatc taggattttt tttgtaacaa ctttgttagc catagcgagc ggtacaatat
    47401 agttatcttt tgaggtcata ttaccatttt taacgtactc aatatgacta ttgtgccata
    47461 attttaggaa ttgatcttgt tcatccagta cttcgaagtt gactcctttt tggataacgt
    47521 caaatagtag caattgggta gaaagttcca tcgtttcata tccctttaag gcgtagggag
    47581 cttcccaagc ataatcgtga taaatttgtc cttgtctttg tccaaatgtt tctaaggaaa
    47641 gtccttcaat catttctaaa agttgaccag ctaccgttaa tttaggtgac tgaatttgcc
    47701 tttttacaga ttccaatagg tcttgatagt aaggtggtaa gttaaaatgt tggataacag
    47761 attgcatagc atccactaag tcagaaactg gtgcttgatt aggtaatttt tctaatggat
    47821 gactgagtgc tataaggtca tttaatctat tggcttcctt gatatcttgg tcaatatgac
    47881 tactagaatc tatccacaaa agcgccaata agaaaaggtg aacagtatca acagtttcct
    47941 gcgttatacc gattgggcta aatgggttga gatcaaaagt gcgaaattct aaatatgtaa
    48001 ttcctttttc taaataatta cgacaggact tactaccacg gagacgaaca ggtgaataaa
    48061 attctttctc agcaatcaat tgcccacttt ttacagcatt ttctagatcg ttgacataat
    48121 ccttcaagct agtataagaa atacgaatat ctttatgatt cacatagcct aagtgactat
    48181 ttctaagtga ccggacaggg ttattaagtt tctgatttaa aaagtcttct tcagctactg
    48241 ggctcgctcc gtatagataa gttagtaacc aacggtaacg taaaaaattt tgagacaatt
    48301 tcatgtaaag ctggttctga aaatcaatag cattatctgc ttgactcaat tcaaagagag
    48361 aagttaataa ctcttgacct aaaccgagat tatagtgaat tccagagatg gattgtatta
    48421 gttttccata agttttctcc aaatatttgc gataatcata ttcaaaagca tcctctaatt
    48481 gggcaatctg tatgtcttct tccctcactt tgggtggcat agaaagcggc cataagtatt
    48541 catcgtggtt gatagatcgt ccagcgacat cacttatagc tttcaaaaat cgaatggctt
    48601 cttggctatc ctttgcaata ggtgtaatga gttctaattg aggttcacta taatccgttt
    48661 ggatataagg atgatagtta cgactcccta atgttttggg atggggtgtt tgagcaaccc
    48721 tttgagtggg ttggtgaata cgaaggcttt ctctctctaa gccaaatgta gcttgtagaa
    48781 tcggtagatg agagtggctt ctttgtaaca gttgatcgat aatcacgaca acattcctcc
    48841 tagtaacaga tgatactagt ttatcaaaaa aataataaaa actaagtttt acggcacaga
    48901 aaactaaaat aatcttatac tggtgtcata aaaaataaaa gattgaactt ttgagtccaa
    48961 tcttattaat ctataagtta aaaagagctt tagagttaat gagtttcaat ttttttaaga
    49021 taacgataga cgcttggttc ggagatgttg agaacttcag cgacctttga aacggcacct
    49081 tttaattgga atacaccttt ttcgtgaagt ttagaaacaa tttctacctt aacttcttga
    49141 cttaaatgaa tattctgatt taaaagacta ggatcaacaa tctcactaat aatatcctga
    49201 atattgcctg atagaacttc tacaggttcc tcctgttgag ggagagaaat tttttgaggg
    49261 gacttagata gtattttgtt aacatttaaa ttaactaagt ctagaatatc caaagcaata
    49321 ttttggtatg cagaaatatc aaggttgata cataacatcc cttcaagttc attttgagca
    49381 ttcttgatga agaaagtaga tccacgaact tctttattga gagggctgac aatcgcctta
    49441 tagttagtaa caaaatcttt ttccttataa actttattct tgattaaatc aagggcgaaa
    49501 gtcgttagag gtgaacttat ggttcgtcca ctaatgtgac tattaaccaa ctcaccaata
    49561 tagatatcat tttcatctac tacatgcaaa acaatttcgt aattatcacc taaaacatta
    49621 tggagaaacg ttattatcgt c
//
LOCUS       DATRQA010000002        39168 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.002, whole genome shotgun sequence.
ACCESSION   DATRQA010000002 DATRQA010000000
VERSION     DATRQA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39168
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.002"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            126..824
                     /gene="coaB"
                     /locus_tag="VBD27_000065"
     CDS             126..824
                     /gene="coaB"
                     /locus_tag="VBD27_000065"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEO7024112.1"
                     /translation="MYNMAMKILITSGGTTEKIDTVRSITNHATGALGKIIAEKYLRE
                     GHQVTLVTTKNAVKPESATNLSIFEIEDVDSLIKTLKPLVKEHDILIHSMAVSDYTPV
                     YMADFEKVKSSDDLDTFLRKDNHEGKISSESEYQVLFLKKTPKVISLVKKWNPQITLV
                     GFKLLVNVTKENLFKVARHSLIKNKATFILANDLIDITSKHHIAYLLDHDNVYKATTK
                     EDIAQLIYEKVKKI"
     gene            821..1363
                     /gene="coaC"
                     /locus_tag="VBD27_000066"
     CDS             821..1363
                     /gene="coaC"
                     /locus_tag="VBD27_000066"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEO7024113.1"
                     /translation="MIKRITLAVTGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNPIHLDVMDEHNPKIINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALAMPDETPKLIAPAMNTKMYHNTITQRNIDILKKIGYQEIEPRISLLACGDT
                     GQGALADISTILKCIQEVAN"
     gene            1409..1981
                     /locus_tag="VBD27_000067"
     CDS             1409..1981
                     /locus_tag="VBD27_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7024114.1"
                     /translation="MKKKNKSSNIAIIAIFFAIMLVIHFLSSFIFSFWLVPIKPTLMH
                     IPVIIASIAYGPRIGATLGALMGGISVANSSIVLLPTSYLFSPFVENGNFYSLIIALV
                     PRILIGIIPYFVYKLLHNRFGLAISGAIGSLTNTVFVLSGIFIFFSSTYNGNIKLMLA
                     GIISSNSLAEMVIAAIIVYLTVPRILNIKH"
     gene            2091..3809
                     /locus_tag="VBD27_000068"
     CDS             2091..3809
                     /locus_tag="VBD27_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO7024115.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVNADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQIATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATVGNDLADAETKIANIEKE
                     ITTFVN"
     gene            complement(3873..4070)
                     /locus_tag="VBD27_000069"
     CDS             complement(3873..4070)
                     /locus_tag="VBD27_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024116.1"
                     /translation="MKKRLQLLLLIFISAVSWFISNQLATKNTVLSVLIGLVAVIGIT
                     WLIVSLLYYLIVKLIAVIFHD"
     gene            complement(4084..5817)
                     /locus_tag="VBD27_000070"
     CDS             complement(4084..5817)
                     /locus_tag="VBD27_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7024117.1"
                     /translation="MKASHPKKIQKKLVQDLLSKKSLVGMALLGTVVQVCLTVYLPVL
                     IGQAVDVVLSPHSMILLLPIMWKMVAVILANTIIQWINPLLYNRLIFHYVASLRKAVM
                     EKLNLLPIAYLDKRGIGDLISRVTTDTEQLSNGLLMVFNQFFVGLLTILVTIFSMAKI
                     DLLMLFLVLFLTPLSLFLARFIAKKSYHLYQNQTASRGRQTQFIEEMVSQESLIQAFS
                     AQEESSDHFRTINQEYANFSQSAIFYSSTVNPSTRFINSLIYGFLAGIGALRIMSGAF
                     SVGQLITFLNYVNQYTKPFNDISSVLSEMQSALACAERLYSILEESSPNITGTEKLDS
                     STVKGQIDFKNVIFGYNKSKPLLNGINLHIPAGAKVAIVGPTGAGKSTLINLLMRFYE
                     VDGGNILLDGKPITDYEPSQLRQEIGMVLQETWLKSATIHDNIAYANPKASREEVIEA
                     AKAANADFFIKQLPNGYDTYLEDAGDSLSQGQCQLLTIARIFLKLPRILILDEATSSI
                     DTRTEVLVQEAFQMLMKGRTSFIIAHRLSTIQTADIILVMVSGEIVEVGNHSELIAQK
                     GIYYQMQNAQK"
     gene            complement(5818..7539)
                     /locus_tag="VBD27_000071"
     CDS             complement(5818..7539)
                     /locus_tag="VBD27_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7024118.1"
                     /translation="MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETI
                     PRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYSSKAAVGYTRQLTEDLYQKVMSLGKK
                     DRDELGTASLITRLTADTFHIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPSLTIWFL
                     IMVVTLFIIVFVMSRLLNPIYLKIRTSTDYLVKLTRQQLQGVRVIRAFNQVDRESEAF
                     NDINYHYTNLQLKAGRLSSLVTPLTFLVVNITLVVIIWRGNLNIANHLLSQGMLVALI
                     NYLLQILVELLKMTMLVTSLNQSYISAKRIIAVFERPSEIIDDKLEPKYSNKALEVQE
                     MAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYKVQEGDIDIYH
                     QGKSPDTISNWRSLVRVVPQNAQLFKGTIRSNLSLGLGKVSEEKLWTALEIAQASDFV
                     KEKDGQLDAPVESFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTEARLF
                     KAIIKHFNQTNLIIVSQRINSIQNADRILLLDKGKQVGFDNHQSLLAHNKVYKSIYHS
                     QNFKEEE"
     gene            complement(7551..8153)
                     /locus_tag="VBD27_000072"
     CDS             complement(7551..8153)
                     /locus_tag="VBD27_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO7024119.1"
                     /translation="MFKFLKRLIALIIIIFIGYRLVIIHENVKKVLQYHDLVQNTLAE
                     NGSEANVHLVLSMIYTETKGDAIDVMQSSESISGTTNSITDSHTSIKHGVTLLSQNIS
                     QAKKAKVDVWTAVQAYNFGSSYIDYVADHGGENSIELAKNYSKNVVAPSLGNYNGDTY
                     FYYHPLALISGGKLYKNGGNIYYSREVQFNLYLIKIMELF"
     gene            complement(8155..9132)
                     /locus_tag="VBD27_000073"
     CDS             complement(8155..9132)
                     /locus_tag="VBD27_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEO7024120.1"
                     /translation="MMDFYIKQIIIHQFSPNDTELVLSDTPLTLTPRIDDYFRKKLSK
                     VFSDEAKRGYFGEDNVFMSHLQDDLYVSSCQIAQLWKEEFVISEDQKTNDLVFIQFDK
                     DGMEHFAFLRISLKEQFAHVSENQEQPITITQNNLPSAAQTPDEALVVNKSSKQYYLI
                     EKRIKHNGSFANYFSENLLQVQPEQSVKKSIKMVEQTAQKIAENFNKDDFSFQSKMKS
                     AIYKNLEEEQELSPEKLADQLFDDNLTARLTFVDELKDAIPGSVQVSDIDHSRQIKKL
                     ENQKLSLSNGIELIVPNNVYQDAESVEFIQNPDGTYSILIKNIQDIQNK"
     gene            complement(9137..10393)
                     /locus_tag="VBD27_000074"
     CDS             complement(9137..10393)
                     /locus_tag="VBD27_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO7024121.1"
                     /translation="MIFDKDNFKEFDQELWQAIHDEEIRQQNNIELIASENVVSKAVM
                     AAQGSVLTNKYAEGYPSHRYYGGTDCVDVVESLAIERAKMLFNAEFANVQPHSGSQAN
                     AAAYMALIEPGDTVLGMDLAAGGHLTHGASVSFSGKTYHFVSYSVDPKTEMLDYDNIL
                     KIAQETQPKLIVAGASAYSRIIDFEKFRQIADAVDAYLMVDMAHIAGLVASGHHPSPI
                     PYAHVTTTTTHKTLRGPRGGLILTNDEAIAKKINSAVFPGLQGGPLEHVIAAKAVALK
                     EALDPSFKIYGEDIIKNAQAMAKVFKEDDDFHLISDGTDNHLFLVDVTKVIENGKKAQ
                     NVLEEVNITLNKNSIPFERLSPFKTSGIRIGTPAITSRGMGVEESRRIAELMIKALKN
                     HENQDVLTEVRQEIKSLTDAFPLYEN"
     gene            complement(10485..11081)
                     /locus_tag="VBD27_000075"
     CDS             complement(10485..11081)
                     /locus_tag="VBD27_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO7024122.1"
                     /translation="MNDLGQILEDHGAVIMPTETVYGIFAKALSEEAVNHVYELKKRP
                     RDKAMNLNICDFETILKYSKNQPTYLKQLYDAFLPGPLTIILEASQEVPDWINSGLSS
                     VGFRMPKHPVTLDMIANHGPLIGPSANISGCDSGRVFSEIQKQFNHQVLGIEDDKALT
                     GVDSTIIDLSGDRVKILRQGAITQEVLTATIPELIFEN"
     gene            complement(11074..11904)
                     /gene="prmC"
                     /locus_tag="VBD27_000076"
     CDS             complement(11074..11904)
                     /gene="prmC"
                     /locus_tag="VBD27_000076"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO7024123.1"
                     /translation="MNYAQLIKHYEQLLEACGEEVENFIYVLKDLKQWSTTDYLLNQN
                     SSVSDTDQQLMENIFQQLKKHRSPQYITGKAYFRDLIFFVDERVLIPRPETEELVDLI
                     LSENKVEDCSVLDIGTGSGAIAISLKKERPSWDVLASDISVSALDLAKENANNCDAEV
                     TFIESDVFSNISDKFDIIVSNPPYISYNDKDEVGKNVLASEPHSALFADEEGLAIYRK
                     IIENSREYLQPRGKLYFEIGYKQGDDLRSLLKRYFPNNRCRVLKDIFGKDRMVVLDNE
                     "
     gene            complement(11904..12983)
                     /gene="prfA"
                     /locus_tag="VBD27_000077"
     CDS             complement(11904..12983)
                     /gene="prfA"
                     /locus_tag="VBD27_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO7024124.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQNISDAEEMIKDASGDAELEEMAKEELKESKAAKEEYEERLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYEI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTQKLEALNK"
     gene            complement(13018..13587)
                     /locus_tag="VBD27_000078"
     CDS             complement(13018..13587)
                     /locus_tag="VBD27_000078"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO7024125.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     EFGVVSSRIGMRREAVPISDDMDIFSYIQNLPQKPYCVLIDECQFLSKKNVYDLARVV
                     DDLDVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            13725..13907
                     /locus_tag="VBD27_000079"
     CDS             13725..13907
                     /locus_tag="VBD27_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEO7024126.1"
                     /translation="MPFVKIDLFEGRSQEQKNELAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGELKKK"
     gene            14056..14994
                     /locus_tag="VBD27_000080"
     CDS             14056..14994
                     /locus_tag="VBD27_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO7024127.1"
                     /translation="MQLSHSLRLMGTTIDIQINSKNAQKQIQEVIELLELYKNRFSAN
                     DFNSELMAINNNAGIKPIQVHPDLFELITIGKEHSLARPSNLNIAIGPLVQTWRIGFS
                     DAKLPSPSEVSEAMILSDPTHILLDSKNQSVFLNQIGMKIDLGALAKGYIADKIMTYL
                     KNEMIDSAIINLGGNVLVHGDNPNRSEGYWVIGIQHPKKKRGQNIGAVKIKNQSVVTS
                     GTYERRLIIDDKEYHHIFDRQTGYPIQTEMASISIVSKQSVDCEIWTTRLFGLSIKEA
                     LDILNAVSYIEGIIITKDDRIYLSDGLKHHFQLFYH"
     gene            15012..15614
                     /locus_tag="VBD27_000081"
     CDS             15012..15614
                     /locus_tag="VBD27_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7024128.1"
                     /translation="MKLIGIVGTNSNKSTNRQLLQYMQQHFADKAEIELIEVKDLPLF
                     NKPADKNVPQVILDIAAKIEEADGVIIGTPEYDHSIPSALMSVLAWLSYGIYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKASVLPDEFLLSHSLQAFDKDGNLHDIET
                     SQKLDAIFDDFRLFVKITGKLSNARDLLQKEAENFDWESL"
     gene            15638..16873
                     /locus_tag="VBD27_000082"
     CDS             15638..16873
                     /locus_tag="VBD27_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7024129.1"
                     /translation="MKFVGIVGSNAEQSYNRMLLEFIRKNFKTKFELEVLEIDDIPMF
                     NQVQNWEESFQLRLLNNKITRADGVIIATPEHNHTITAALKSVLEWLSFAVHPLENKP
                     VMIVGASYYDQGTSRAQIHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDNGNIINPG
                     TVQFLETCLDNFIKYVGVVSSLRKPKAIESEDLYCQGQIDTTITSIDPDDPDWLDKAA
                     EKVNAVEGDAYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNNHPDPDTML
                     GKRVREQVGNRMATVHGSLPPARMKNVEWVIGTLRNGNQEYVRTVVPGTPPEVINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLQTTGQRLVGGTTAAPGASAVDATSSASTS
                     TEDAPVDQATDATSGASEH"
     gene            16972..17760
                     /locus_tag="VBD27_000083"
     CDS             16972..17760
                     /locus_tag="VBD27_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEO7024130.1"
                     /translation="MSLFQEKIAYNCAKKEALYKESLGRYALRSMLAGAYLTMSTAAG
                     IVAADTIGKISPALSGFVFAFIFSFGLIYVLIFNGELATSNMLYLTAGAYNKNISWKK
                     AITILIYCTFFNLVGACILAWLFNQSYSFQHLTNDSFLGHVVAKKLGKPSSGAFLEGI
                     IANMFVNLAILAYMLLKEESAKMTVILSAIFMFVFLSNEHLIANFASFMLAAFSHIEH
                     IKGFTLLNVIRQWTLVFFGNWIGGGVFIGLAYAWLNKTKTSHHE"
     gene            complement(17859..19133)
                     /locus_tag="VBD27_000084"
     CDS             complement(17859..19133)
                     /locus_tag="VBD27_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEO7024131.1"
                     /translation="MKFNEQSNSQAALLGLQHLLAMYAGSILVPIMIASALGYNAKQL
                     TYLIATDIFMCGIATLLQLRLSKHFGVGLPVVLGCAFQSVAPLSIIGAQQGSGYMFGA
                     LIASGIYVVLVAGIFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKEPSLQSL
                     TLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVAEAPLVHIPK
                     PFYFGAPRFEFTSILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLA
                     VLLSGLFNTFPYTGFSQNVGLVQISGIRTRKPIYFTALFLVILGLLPKFGAMAQMIPS
                     PVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNETNLFISLPN
                     TLQMFLTNGIVISTLTAVVLNIILNGLPKKLI"
     gene            complement(19133..19714)
                     /locus_tag="VBD27_000085"
     CDS             complement(19133..19714)
                     /locus_tag="VBD27_000085"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEO7024132.1"
                     /translation="MKLLEERILKDGDVLGENILKVDSFLTHQVNFELMQEIGKVFAD
                     KYKEAGITKVVTIEASGIAPAVYAAQALGVPMIFAKKAKNITMTEGILTAEVYSFTKQ
                     VTSQVSIVSRFLSNDDTVLIIDDFLANGQAAKGLLEIIGQAGAKVAGIGIVIEKSFQD
                     GRDLLEKTGVPVTSLARIKAFENGRVVFAEADA"
     regulatory      complement(19818..19913)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(19969..20952)
                     /gene="guaC"
                     /locus_tag="VBD27_000086"
     CDS             complement(19969..20952)
                     /gene="guaC"
                     /locus_tag="VBD27_000086"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEO7024133.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIISSRSQADTSVKLGNYTFKLPVI
                     PANMQTIIDEEVAETLACEGYFYIMHRFNEEARRPFIKRMHDKGLIASISVGVKDYEY
                     DFVTSLKEDAPEFITIDIAHGHSNSVIEMIQHIKQELPGTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCSKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHLESPGKLVEVEGQQFKEYYGSASEYQKGEHKNVEGKKIL
                     LPVKGRLEDTLTEMQQDLQSSISYAGGKELDSLRHVDYVIVKNSIWNGDSI"
     gene            complement(21156..22496)
                     /locus_tag="VBD27_000087"
     CDS             complement(21156..22496)
                     /locus_tag="VBD27_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7024134.1"
                     /translation="MTKKGWRIMPAIITTAILGFSGILIETSMNVTFPLLMKEFGVNP
                     AVIQWVTTGNLLAVAVTVPLSAFMIKNLSERQIFTLANVLFLFGVLIDSFAPNLAILL
                     VGRVLQGVGTGLALPLLFHIILTQIPMERRGLMMGVAAMVTLLAPAVGPTYGGVISGM
                     LGWKMIFMLLAPILIISTFIGLASIPKRQVRINDKLNFPAFVSLGIGLATLLLAIEKM
                     SIFYLLVAIVSFVIFYYLNKQLEFLNLNVFKDKDFSILLYGVLAFQMIPLALSFLLPN
                     LLQLVLHQTSTKAGLFMFPGAIAVVFLSPFAGYLLDKIGAFKPIMIGISLSLIGLIGT
                     AIFIPAKFVVVLLAFDILTKIGMGIGASNMVTTALTKLKPAQSADGNSILNTLQQFAG
                     AFATAVASQIFTIGQVAIPKNGAIIGSQFAVLFVIVVVILAIVGLTYLRKRKAI"
     gene            22788..23459
                     /locus_tag="VBD27_000088"
     CDS             22788..23459
                     /locus_tag="VBD27_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024135.1"
                     /translation="MAFASNESENNFFITLKTYFNYLFSIQIITDISTLNHADFDGSF
                     AFHDIETSIPHLVIDSNYLAISQTNSKTEANDIKTFSELSKTMTEFYYMLNFDLFNHL
                     PYRFRLHNKDGQTIYSNHKPEDPFDIYPEEEYPIDKWVQNSLIEKKAKELHLLLPGAS
                     QDYILVQSYKRLENDSGQLVGYIEHVHNIKPLLEGYLKESGQAIVGWSDVTSGASISN
                     DDFEL"
     gene            complement(23530..25530)
                     /locus_tag="VBD27_000089"
     CDS             complement(23530..25530)
                     /locus_tag="VBD27_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO7024136.1"
                     /translation="MQHVNHSSFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GISSVTESFILGSISLIIWTLTLITTIKYVLVALKADNHHEGGIFSLYTLVRKMTPWL
                     IVPAVIGGATLLSDGALTPAVTVTSAVEGLKVVPSLQHIFQNQSNVIFATLFILLLLF
                     AIQRFGTGVIGKLFGPIMFIWFAFLGISGLLNSFAHPEVFKAINPYYGLKLLFSPENH
                     KGIFILGSIFLATTGAEALYSDLGHVGRGNIHVSWPFVKVAIILSYCGQGAWILANKN
                     AGNELNPFFASIPSQFTMHVVILATLAAIIASQALISGSFTLVSEAMRLKIFPQFRST
                     YPGDNIGQTYIPVINWFLFAITTSIVLLFKTSAHMEAAYGLAITITMLMTTILLSFFL
                     IQKGVKRGLVLLMMIFFGILEGIFFLASAVKFMHGGYVVVIIAVAIIFIMTIWYKGSK
                     IVSRYVKLLDLKDYIGQLDKLRHDHRYPIYHTNVVYLTNRMEGDMIDKSIMYSILDKR
                     PKKAQVYWFVNIKVTDEPYTAEYKVDMMGTDFIVKVELYLGFKMRQTVSRYLRTIVEE
                     LLESGRLPKQGKTYSVRPDSKVGDFRFIVLDERFSSSQNLKPGERFVMLMKSSVKHWT
                     ATPIRWFGLQFSEVTTEVVPLIFTANRGLPIKEKIELTTTGD"
     gene            complement(25665..26425)
                     /locus_tag="VBD27_000090"
                     /pseudo
     CDS             complement(25665..26425)
                     /locus_tag="VBD27_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649058.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(26487..27479)
                     /gene="pta"
                     /locus_tag="VBD27_000091"
     CDS             complement(26487..27479)
                     /gene="pta"
                     /locus_tag="VBD27_000091"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEO7024137.1"
                     /translation="MRSLFGGLREKVASDYVKIVFPEGSDERVLRAAARLKFEGLVDP
                     VILGKADEVHDSLARLGFVDQDYSVIDPEQYEKFEEMKEAFVEIRKGKATMEDADRLL
                     KDVNYFGVMLVKLGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRENT
                     QERYIFADCAINIDPNAQELAEIAVNTADTAKIFDINPKIAMLSFSTKGSAKAPQAEK
                     VQEAAKIAKDLSPELAVDGELQFDAAFVPETAEIKAPNSDVAGKANVFIFPDLQSGNI
                     GYKIAQRLGMFEAIGPILQGLNAPVNDLSRGSSAEDIYKLAIITAAQAIDEKSK"
     gene            complement(27505..28389)
                     /locus_tag="VBD27_000092"
     CDS             complement(27505..28389)
                     /locus_tag="VBD27_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7024138.1"
                     /translation="MKFEYIAEERCKVKTLLKSHDVSRGLLAKIKYRGGKIFVNGEEQ
                     NAIFLLEIGDVVTIDIPDEPSHETLEPVPHDLDIVYEDDHFLILNKPFGFASIPSSIH
                     SNTIANFIKHYYVSNNYANQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQAKAIE
                     KRYYALVSGSGDLADSGDIIAPIARDVDSIITRRVHESGKYAHTSYQVVARYGDVRLV
                     DIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRMDLGINRQALHCHSLSFYDPFMGKIN
                     KQTLDLTDDFDSVIMELH"
     gene            complement(28386..29222)
                     /locus_tag="VBD27_000093"
     CDS             complement(28386..29222)
                     /locus_tag="VBD27_000093"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEO7024139.1"
                     /translation="MTQMNFTDRATRVAIIANGKYQSKRVASKLFAAFKHDPDFYLSK
                     KDPDIVISIGGDGMLLSAFHMYEKQLDKVRFVGVHTGHLGFYTDYRDFEVDTLINNLK
                     NDKGEQISYPILKVTITLEDGRVIRARALNESTIKRIEKTMVADVVINQVVFERFRGD
                     GILVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVYRTLGSSVIIPKKDAIE
                     IVPKRVGVYTISIDNKTVHYKNVTKIEYSIDEKSINFVSTPSHTSFWERVNDAFIGEP
                     EH"
     gene            complement(29197..29868)
                     /locus_tag="VBD27_000094"
     CDS             complement(29197..29868)
                     /locus_tag="VBD27_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO7024140.1"
                     /translation="MSMDWETFLDPYIQTVGELKIKLRGIRKQFRKQNRHSPIEFVTG
                     RVKSVESIQEKMVLRGISEENLAQDLQDIAGLRIMVQFVDDVDEVLALLRKRHDMTVV
                     QERDYITHMKSSGYRSYHVVVEYPVDTIDGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPEEIKQRLEKTAKIALELDEEMRKIREDIREAQLLFDPLNRKLSDGVGNSDDT
                     DEFYR"
     gene            29978..30550
                     /locus_tag="VBD27_000095"
     CDS             29978..30550
                     /locus_tag="VBD27_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEO7024141.1"
                     /translation="MTHLEIEYKTLLNKDEFNRLTSLFSHVQPITQTNYYFDTETFEM
                     KAHRMSLRIRTLPNRAELTLKIPREVGNLEHNHDLTLEEAKYIVKNGQFPEDTEIASL
                     ILEKGVDPTKLAVFGQLTTTRREMETSIGLMALDSNIYADIKDYELELEVKQPKQGKR
                     DFDQFLKENNINFKYAKSKVARFSATLKNN"
     gene            30727..31701
                     /locus_tag="VBD27_000096"
     CDS             30727..31701
                     /locus_tag="VBD27_000096"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO7024142.1"
                     /translation="MAEQYADKQIKLFSLTANREIAEKISQASGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVVPYFGYSR
                     QDRIAASREPITAKLVANMLVKAGVDRVLTLDLHAVQVQGFFDIPVDNLFTVPLFAEH
                     YNQLGLSGEDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREEGYIIGEVEG
                     KKAIIIDDILNTGKTFAEAAKILERGGATEIYAVASHGLFAGGAADILESAPIREIIV
                     TDSVLSKERIPSNIKYLTASHLIADAIIRIHERKPLSPLFSYRSDKKD"
     gene            31705..32820
                     /locus_tag="VBD27_000097"
     CDS             31705..32820
                     /locus_tag="VBD27_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7024143.1"
                     /translation="MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQL
                     LRECRQIIAEYLNVNSREIIFTSGGTESNNTAIKGYALANQLKGKHIITSEIEHHSVL
                     HTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTIMVSLMFANNETGDFLPIQEI
                     GQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFD
                     SLLHGGDQEEKRRASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLD
                     FYLNNGQDCLPHVLNIGFPRQNNGLLLTQLDLAGFAVSTGSACTAGTVEPSHVLTSLY
                     GANSPRLNESIRISFSELNTQEEILELAKTLRKIIGD"
     gene            32822..33169
                     /locus_tag="VBD27_000098"
     CDS             32822..33169
                     /locus_tag="VBD27_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEO7024144.1"
                     /translation="MAFEKEINLPDCNYIYTISPNIKKYTLRDTTFTQNRLGNYELER
                     LLETVPNSGDGFPLKITINKELSGFKLLITDKSGLRIVNIFKSTDHKILQDKFYFLMD
                     SLVERNIFTKTAK"
     gene            33324..33962
                     /locus_tag="VBD27_000099"
     CDS             33324..33962
                     /locus_tag="VBD27_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO7024145.1"
                     /translation="MIMDKSIPKATAKRLSLYYRIFKRFNTDGIEKASSKQIADALGI
                     DSATVRRDFSYFGELGRRGFGYDVKKLMNFFAEILNDHSTTNVMLVGCGNIGRALLHY
                     RFHDRNKMQISMAFDLDSNDLVGKTTEDGIPVYGISTINDHLIDSDIETAILTVPSTE
                     AQEVADILVKAGIKGILSFSPVHLTLPKDIIVQYVDLTSELQTLLYFMNQQR"
     gene            complement(33975..34655)
                     /gene="radC"
                     /locus_tag="VBD27_000100"
     CDS             complement(33975..34655)
                     /gene="radC"
                     /locus_tag="VBD27_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO7024146.1"
                     /translation="MYHIELKKEALLPRERLVDLGADRLSNQELLAILLRTGIKEKPV
                     LEISTQILENISSLADFGQLSLQELQSIKGIGQVKSVEIKAMLELAKRIHKAEYDRKE
                     QILSSEQLARKMMLELGDKKQEHLVAIYMDTQNRIIEQRTIFIGTVRRSVAEPREILH
                     YACKNMATSLIIIHNHPSGSPNPSESDLSFTKKIKRSCDHLGIVCLDHIIVGKNKYYS
                     FREEADIL"
     gene            complement(34751..35884)
                     /locus_tag="VBD27_000101"
     CDS             complement(34751..35884)
                     /locus_tag="VBD27_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO7024147.1"
                     /translation="MKFEKRQVYYVVITFAICYAIQAYWGAVSNILTTLHKAIFPFLM
                     GAGIAYIINIVMSVYERLYIKLFKGSRLLMAIKRSVSMILSYATFIGLIVWLFSIVIP
                     DLISSLSSLLVIDTGALAKLVNNLNENKQISEALNYMGTDKDLVSTLSGYSQQILKQV
                     LSVLTNLLTSVSSIAATLLNVFVSFIFSIYVLANKEQLGRQFNLLIDTYLGSTGKTFH
                     YVRHILHQRFHGFFVSQTLEAMILGSLTVIGMLIFQFPYALTVGVLVAFTALIPVVGA
                     YIGVTIGFILIATESLTEAFLFVLFLILLQQFEGNVIYPKVVGGSIGLPSMWVLMAIT
                     IGGALWGILGMLLAVPVAATIYQIVKDHIIKRQTLRNRARTYR"
     gene            complement(35975..37414)
                     /locus_tag="VBD27_000102"
     CDS             complement(35975..37414)
                     /locus_tag="VBD27_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO7024148.1"
                     /translation="MMVKQVFPKGFLWGGATAANQCEGAYNVDGRGLANVDVVPTGED
                     RFAIISGQKKMFDFEEGYFYPAKESIDFYHHYKEDLALLAEMGFKTYRMSIAWTRIFP
                     KGDELYPNEAGLRFYENIFKECRKYGIEPLVTITHFDCPIYLIKHYGGWRSRKMIGFY
                     ERLVRALFTRFKGLVKYWLTFNEINMILHAPFMGAGLYFEDGENQEQIKYQAAHHELV
                     ASAIAVKIAHEVDPNSQIGCMLAAGQYYPNTCHPQDYWASMQKNRENYFFIDVQARGK
                     YPNYAKKYFEHLGISIQMTAEDLALLRDYTVDFISFSYYSSRVASGNPTVSEQVQENI
                     FASLKNPYLKSSEWGWQIDPLGLRITLNAIWDRYQKPMFIVENGLGAVDIPDENGYVE
                     DDYRIDYLRQHIAAMRDAIYVDGVNLIGYTTWGCIDLVSAGTGEMEKRYGLIYVDRNN
                     KGEGTLKRYKKKSFYWYKKVIASNGSQIE"
     gene            complement(37574..38188)
                     /locus_tag="VBD27_000103"
     CDS             complement(37574..38188)
                     /locus_tag="VBD27_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEO7024149.1"
                     /translation="MLEVIDKALRDYQMKREQFFEINNQTVQEGAIVFTGDSIVEFFP
                     LKKHLGRDYPLVNRGVAGSDSYWLLENLRTQVWELLPSKVFILIGTNDIGLGHSQSEI
                     IANITDIIAEIRAESYMTEINILSVLPVSEEDDYIERVKVRNNQAIKALNKTLSVISG
                     INYIELYDLLVDEKGQLASSFTKDGLHLTDQAYAKISETIKLHL"
     gene            complement(38289..39110)
                     /locus_tag="VBD27_000104"
     CDS             complement(38289..39110)
                     /locus_tag="VBD27_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7024150.1"
                     /translation="MIKLIATDMDGTFLRSDKTYDKARFSSLLTLMEKYDIKFVAASG
                     NLYDQLLLNFLEYPNRIAYVAENGGRVIDQDGTLLKETYLSNDTVAAVLSYLYQNYPE
                     TLISLSGEKRSYLERRTPINRRTELEYYMPNFIYKDHLLPLDDDRYFQMTLWVNENLV
                     SEMLLDISEHFKNHHIRLTSSGFGCIDVLPADVNKADGIAILLEKWGLKQDQVMVFGD
                     GGNDVEMLRAANISYAMSNAPEEIKAIAKYQTVSNDQDGVLETIENFLHKMSDNE"
BASE COUNT        12993 a         7501 c         6135 g        12539 t
ORIGIN      
        1 ctttctctag aaaactctgc aagatttatt ttatcgcaat cgcttgaaat tttccagtaa
       61 gtttccctca aattccatta ctatcaagaa gaaatatatc ttttatttac gaaaagcatt
      121 tttttatgta caatatggct atgaaaattt tgattacatc aggtggaact accgagaaaa
      181 ttgataccgt taggagtatc acgaatcacg caacaggagc tttaggaaaa ataattgcag
      241 aaaaatattt acgagaagga catcaggtga cactcgtgac tactaaaaat gcagtcaaac
      301 cagaatctgc taccaactta tccatatttg agattgagga cgttgactca ttaataaaaa
      361 ccttgaagcc attagtgaaa gagcatgata ttttaattca tagcatggca gtgtctgact
      421 atacccccgt ctacatggct gatttcgaaa aggttaaatc aagcgatgat ttagatactt
      481 ttttgcgcaa agataaccac gaagggaaaa tatcatctga atcagaatat caagtccttt
      541 ttctcaaaaa aacaccaaaa gttatcagcc ttgtgaaaaa atggaatcct caaattactc
      601 tagtaggttt taaactactt gtgaacgtta ctaaagaaaa cctttttaaa gttgctcgtc
      661 atagcttaat caaaaataaa gctacgttta ttttagctaa tgacctcatt gatattacct
      721 caaaacatca catagcctac ctacttgatc atgataatgt gtataaagct accaccaaag
      781 aagatattgc tcaattaatt tacgagaagg taaaaaaaat atgattaaac gtattacact
      841 agccgtcaca ggttcaattt cggcctataa agcagcagac cttacaagtc aattaactaa
      901 aattggctat gatgttcata tcataatgac acaagctgcc acacaattta ttacaccgtt
      961 aactctgcaa gtattatcaa aaaatcctat tcatttagat gttatggatg agcataaccc
     1021 aaaaatcatc aaccatatcg aattagcaaa acgcacagat ttatttatcg ttgcacctgc
     1081 ctctgctaat actattgctc atctagcata tggcttcgct gataacatcg taacaagcgt
     1141 tgctcttgct atgcccgatg aaactccaaa acttatcgca ccagcaatga atacaaagat
     1201 gtatcataat acaatcaccc aacgtaacat tgatattttg aaaaaaatag gttatcagga
     1261 aattgaacct cgtattagtt tgcttgcctg tggtgatacc ggtcaaggag ccctagctga
     1321 tatctctact atacttaaat gtatacaaga agttgcaaat taggctgtaa atttgtatac
     1381 tatttctagt ttaatttaag gagttataat gaaaaagaaa aacaaatcct ctaacattgc
     1441 tataattgca atcttttttg ctattatgct tgtcattcat tttttgtcat catttatttt
     1501 tagtttttgg ttagtcccta ttaaacctac tttgatgcat atcccagtta ttattgcatc
     1561 tatagcctat ggacctcgta ttggtgcaac tctaggcgcc ttaatggggg ggatcagcgt
     1621 agctaacagc agcattgttc tattaccaac gagttacctc ttctcacctt ttgttgaaaa
     1681 tggtaatttt tattcgctaa ttattgcact tgtaccacgt attctaatcg ggattattcc
     1741 ttatttcgtt tacaaattac tacacaaccg ctttggtttg gctatctcag gtgctatagg
     1801 ctctctaaca aacacagtat ttgttttatc tggaattttt atcttttttt caagtactta
     1861 taatgggaat atcaagctaa tgctcgctgg gattatttca tctaattcat tagctgagat
     1921 ggtcattgca gctatcattg tatatctaac tgttcctcgt attctcaata ttaaacatta
     1981 aaaactggcc aggaagaaat cttcctggct tttcttactt atattttata gtcatattga
     2041 aaaaaacatg ttataatgaa agcgtttata aatattgaag gagtatttca atgacttaca
     2101 cagaaaattt tcaaaaatgg ttagattttg agcagcttcc tgactatctt cgtcaagaat
     2161 tactatcaat ggatgaaaaa acaaaagaag atgcttttta cacaaactta gaatttggta
     2221 ctgctggtat gcgtggttat attggtgctg gtacaaatcg tattaatatt tatgttgtcc
     2281 gtcaagcaac cgaaggattg gcaaaattaa tcgaaactaa gggagaggaa gctaaaaaac
     2341 gtggtgtagc cattgcttat gattcacgcc acttctctcc agaatttgct tttgagtctg
     2401 cacaggtctt ggcacagcat ggaattaaat cttacgtatt tgaagctctt cgtccaacac
     2461 cagaactctc ttttgctgtt cgccatttaa atgcctatgc tggtattatg gttactgcaa
     2521 gccacaatcc agcacctttc aatggttata aagtctatgg acaagacgga ggtcaactac
     2581 caccagctga tgctgatgca ctaacagact ttatccgtgc tattgaaaat ccatttgcag
     2641 ttgaacttgc tgatttagat gaaagcaaat catcgggcct tattcaggtt atcggtgaag
     2701 atgttgatat tgaatatctt agagaggtta aagatgttaa tatcaatcaa gatttaatca
     2761 ataactttgg aaaagacatg aaaattgtct atactcctct ccacggaact ggtgagatgt
     2821 tgacacgccg tgcccttgct caagccggtt ttgaatctgt tgttgtagta gaatcacaag
     2881 ctaaggcaga tccagacttt tctactgtta aatcgcctaa ccctgaaagc caagctgcct
     2941 ttgctcttgc tgaagaactc ggacgagaag ttaatgcaga tgtcttagta gctactgatc
     3001 ctgatgcaga ccgcctaggc gttgaaattc gccaaccaga tggttcttat aaaaaccttt
     3061 ctggaaatca aattggagcg attattgcga aatacatcct ggaagctcat aaaacagcag
     3121 gaacacttcc agaaaatgct gctctcgcta aatcaatcgt atcaacagaa ttagtaacaa
     3181 aaattgccga aagttatggc gctacaatgt ttaacgtttt aactggattc aaattcatcg
     3241 ctgagaaaat tcaagagttt gaagaaaaac ataaccacac ctatatgttt ggttttgaag
     3301 agagcttcgg atatcttatt aaaccatttg tccgtgataa agacgctatt caagcagttc
     3361 ttttggttgc tgagattgca gcttattacc gttcgcgtgg tttaacactt gcagatggta
     3421 ttgatgaaat ttacaaagaa tatggttatt ttgctgaaaa aacgatttca gtaacacttt
     3481 caggagtgga tggagctgct gaaattaaga agattatgga caaattccgt gaaaatggtc
     3541 caaaacaatt taacaatact gatattgtat tattagaaga tttccaaaaa caaatcgcta
     3601 ctaaaaatga tggaacaata tctaacttaa caactcctcc aagtaatgtt ttgaagtaca
     3661 cgttagctga tgattcatgg attgcagttc gcccttcagg aactgaacca aaaatcaagt
     3721 tctatattgc tacggtcggt aatgatttag cagatgcaga aacaaaaatc gccaatattg
     3781 aaaaagagat tacaacattt gtaaattaat aaaaaaagct aggaacttac tcctagcttt
     3841 ttttatatct atctaacgat gttctattat tttcaatcat gaaatataac tgcaattaac
     3901 ttcacgatca aataatagag cagacttaca attaaccacg ttatcccaat aactgcaact
     3961 aaacctatta aaacagataa taccgtattc ttagttgcta gttggttact gataaaccat
     4021 gaaactgcag aaataaatat cagtaataat agttgaagcc tttttttcat ataattcctc
     4081 ttactacttt tgtgcatttt gcatttgata ataaatacct ttttgagcta ttaattcaga
     4141 gtggttaccc acttctacaa tttcaccaga taccataact aaaataatat ctgctgtttg
     4201 aatggtagat aagcgatgtg caataataaa gcttgttctc cccttcatca acatttggaa
     4261 tgcttcttga acaagaactt cagtacgagt atctatagac gatgtcgcct catctaatat
     4321 caatattcta ggtaatttaa gaaaaatacg agcaattgtc aatagctgac attgcccctg
     4381 tgataacgaa tctccagcat cttctaaata cgtatcatat ccattcggta attgtttgat
     4441 aaagaaatct gcattagctg cttttgcagc ctctatcact tcttcacggc tagctttagg
     4501 attggcatat gcaatgttat cgtgaatcgt agcacttttt aaccacgttt cttgtaatac
     4561 cattccaatt tcttgcctta attgtgatgg ctcataatca gtaataggct taccatctaa
     4621 aaggatattt cccccgtcaa cttcatagaa acgcattaat aaattgatta aagtagactt
     4681 cccagcacct gttggtccta caatagcgac cttagctcct gctggaatgt gtaaattaat
     4741 accattcaat agtggttttg atttattata accaaaaata acgtttttaa aatcgatttg
     4801 acctttgacc gttgatgaat caagtttttc tgtacctgtt atatttggtg acgattcttc
     4861 taaaatagag taaaggcgtt ctgcacaagc cagagcactt tgcatctcag acaggacgga
     4921 agagatgtca ttaaatggct tagtatattg attgacataa tttaagaaag tgattaattg
     4981 accaacacta aaagcacctg acataatacg aagggcacca atccctgcta agaagccata
     5041 aatcaaacta ttgataaatc gggttgatgg attgacagtt gaagaataaa aaattgctga
     5101 ttgagaaaag ttagcatact cttgattaat agtcctaaaa tgatcactac tttcttcttg
     5161 cgcactgaat gcttgaataa ggctttcttg actcaccatc tcttcaatga attgagtttg
     5221 tcgtcctcgt gatgccgttt gattttgata taagtgataa ctctttttag caataaagcg
     5281 ggctagaaag agtgataaag gcgtcagaaa taataccaaa aaaagcatta ataaatcaat
     5341 ctttgccata ctaaaaatag taactagaat agttaataaa ccaacaaaga actggttaaa
     5401 aaccattaat aaaccatttg ataattgctc cgtatcggta gtcacacgac taatcaagtc
     5461 acctatgcca cgtttatcaa ggtaagctat aggtaataga ttcaactttt ccattacagc
     5521 ctttcttaaa gaagcaacat aatgaaaaat taagcggtta tagagcagag gattaatcca
     5581 ttgaataata gtatttgcta aaataactgc aaccatcttc cacattatag gtaacaataa
     5641 tatcatagaa tgaggcgata atacaacgtc taccgcctgt ccaattagaa caggcaaata
     5701 aacagtgagg caaacttgta ctaccgtccc taataatgcc attcctacta agcttttttt
     5761 agaaagaaga tcttgaacaa gctttttttg tatcttttta ggatgacttg ctttcattta
     5821 ctcctcctcc ttaaaatttt gtgaatggta aatggactta taaactttgt tatgagctaa
     5881 taaagattga tgattatcaa aaccaacttg tttgccctta tcaaggagta agattctatc
     5941 tgcattttgt atactattaa ttctctgtga tacaataata agattagttt ggttgaaatg
     6001 tttaattata gctttaaata aacgagcttc tgttaaataa tccaatgcag atgttgcatc
     6061 gtctaaaatc aaaaatggaa tcttatcttg aactaaagct cttgcaatcg tcaacctttg
     6121 cctttgacca ccagagaaat tcctaccaaa actctctaca ggggcatcaa gttgaccatc
     6181 tttttctttt acaaaatcac tagcttgtgc tatttctaaa gcagtccaaa gtttttcctc
     6241 actaacttta ccaagtccca aagaaaggtt agaacgaata gttcctttaa acaactgagc
     6301 attttgagga acaactctta ccaggctacg ccaatttgaa attgtatctg gactttttcc
     6361 ctgatgatat atatcaatat ccccttcttg cactttataa atatgaagca gtaaattaac
     6421 caaggttgat ttcccagagc cagttccacc aattatccct aaagtttctc ctacattcat
     6481 agaaaaagta atatcagata aagctttttc agaggagttt ggataagaaa atgccatttc
     6541 ttgcacttct aaagccttat tcgaatattt tggctcaagt ttgtcatcaa ttatttcaga
     6601 cggtctttca aaaacggcta taattcgctt agcactgata taactttggt tcagtgatgt
     6661 caccaacatt gtcattttta acaattcaac aagaatttgc aataagtaat taattaaagc
     6721 aaccaacatt ccttgtgata aaagatgatt agctatattt aaattaccac gccaaataat
     6781 cacaaccaaa gtgatattaa caactagaaa tgttagaggc gttactaaac tagaaagcct
     6841 acctgctttt aattgtaaat tcgtatagtg ataattgata tcattaaatg cttcactctc
     6901 tctatctact tgattaaagg cacggataac tcgtacacct tgaagttgtt gcctagtcaa
     6961 ctttaccaaa taatcggttg aagttctgat tttaaggtaa ataggattta atagtcgaga
     7021 cattacaaaa acaatgataa ataacgtgac aaccattatc aagaaccaaa tagtcaatga
     7081 gggactaatg gaaaaagcca taattatggc accaaaaaca ataatcggag ccctcaaaaa
     7141 taaacgtaaa aattgattta aaccagtttg gatgtgaaat gtatcagcag tcaaacgagt
     7201 aatcaaacta gcagttccca attcatctct gtctttttta cccagtgaca tgactttttg
     7261 atagagatcc tctgtcaatt gtctcgtata accaactgcg gcttttgaag aataatattg
     7321 agcagttatc gctactacaa cacccaccgc agccaaaaag aaaatcaatc caatttgcaa
     7381 cagtaaacta cttctatccc ctcgtggaat ggtctcgtca atcatttttg caataacgat
     7441 tggtaccaaa agttcaaaag aggcttctaa taatttgaaa aggggtccaa agatagtttc
     7501 tttgatatac cccttaaaat aataagttag tcttttcata cttacccttc ttaaaacaac
     7561 tccattattt ttatcaaata aaggttgaat tgaacttcac gtgagtagta aatattacca
     7621 ccatttttat agagcttccc tcctgaaatt aaggcgagag gatgatagta aaagtaagtg
     7681 tccccgttat aatttcctag gctgggagct acaacatttt tagagtaatt tttggccaac
     7741 tcaatggaat tttcaccccc atgatccgct acataatcaa tataagaaga accaaaatta
     7801 tatgcttgga cagcagtcca tacatcaacc ttagcttttt tagcttgact tatattttgt
     7861 gacaacagag tgacgccatg tttaatactg gtgtgactat ccgtaatact gttcgtagtt
     7921 ccactgatac tttcacttga ttgcatcaca tctatagcat ctcccttagt ttcagtataa
     7981 atcatagata atactaagtg cacattagcc tctgaaccat tctctgcaag agtattctgc
     8041 actaaatcat gatattgaag cactttttta acattttcat ggatgataac tagacggtac
     8101 ccaatgaaaa taataataat taatgctatc aaacgtttta aaaatttaaa catattattt
     8161 attttgaata tcctgtatgt ttttgattag tattgagtat gtaccatcag ggttttggat
     8221 aaattcaact gattcggcat cttggtagac attgttgggt acaatcaatt caataccatt
     8281 tgaaagagat agcttctgat tctctaactt cttaatttga cgggaatgat caatatcact
     8341 tacttgaaca gatccaggta ttgcgtcttt caattcatca acaaaagtca gcctggctgt
     8401 taaattatca tcaaataatt gatctgctaa tttttctgga gacaattctt gctcttcttc
     8461 taaattctta taaatagccg atttcatttt tgattggaaa gaaaaatcat ctttgttgaa
     8521 attttcagca attttttgag ctgtctgttc aaccattttt atagattttt taacagactg
     8581 ttctggttgt acttgtaata aattttcaga aaaataatta gcaaatgaac cattgtgttt
     8641 gatacgcttt tcaattaaat agtattgctt tgatgacttg ttaacaacca aagcttcatc
     8701 tggagtttga gcagcacttg gaaggttatt ctgtgttatt gtaattggct gctcttgatt
     8761 ttcagaaaca tgagcaaatt gttcctttaa tgagattcga aggaaagcaa aatgttccat
     8821 tccatccttg tcaaattgaa taaaaactaa gtcattcgtt ttttgatctt ccgatatgac
     8881 aaactcttct ttccaaagtt gcgcaatttg acaggaagaa acatataaat catcttgtaa
     8941 atgactcata aatacattat cctctccaaa atagccacgc ttagcttcat cagaaaaaac
     9001 tttacttaat tttttacgaa agtagtcatc aatccttgga gttagggtta acggagtatc
     9061 tgatagcact aactctgtgt cgttaggaga aaactgatgt ataataatct gtttgatata
     9121 aaaatccatc ataacattaa ttctcataca aagggaaagc gtccgttaat gatttaattt
     9181 cttgacgaac ttctgtgagg acatcttgat tttcatgatt tttgagtgcc ttaatcatta
     9241 attcagcgat tcttcgagat tcttcaactc ccattcctcg actagtaatt gctggagtac
     9301 caattcgaat acctgatgtt ttaaatggtg atagtcgctc aaacggtata gagttcttgt
     9361 taagggtgat attgacttct tcaagaacat tttgtgcctt tttgccattc tcaataactt
     9421 ttgtgacgtc tactaagaac aaatgattat cagtaccatc agaaatcaaa tgaaagtcat
     9481 catcttcttt aaaaacttta gccatggctt gagcattttt aataatatcc tcaccgtata
     9541 ttttaaagga aggatctaga gcttctttca aagctacagc ttttgcagca attacatgtt
     9601 ctaatggtcc gccttggagt cctggaaaga ctgcagaatt aattttttta gcaattgctt
     9661 catcattggt taaaatcaaa ccacctctag gtccacgtag agtcttatgt gttgtcgttg
     9721 tcgttacatg agcataagga ataggactag gatgatgacc agaagcaact aaacctgcaa
     9781 tgtgagccat gtcaaccatt aagtaagcat ctactgcgtc tgctatttga cgaaactttt
     9841 caaaatcaat aatgcgagaa taggccgacg cccctgcaac aattaattta ggttgcgttt
     9901 cctgtgctat tttcaaaatg ttatcatagt ctagcatctc cgtcttagga tcaacagagt
     9961 aagaaacaaa atgataagtt tttcctgaga agctaactga agcgccatgt gttaagtgcc
    10021 cacctgctgc taagtccatc cctaagacag tatctcctgg ctcgattaaa gccatgtaag
    10081 ctgctgcatt agcctgacta ccagaatgtg gttgaacatt agcaaactca gcattaaata
    10141 acatcttagc tcgttctata gctaaacttt caactacatc aacacaatct gttcctccat
    10201 aataacgatg actaggatac ccttcggcat atttatttgt caaaacagaa ccttgtgctg
    10261 ccataacagc ttttgaaaca acgttttctg atgcaattaa ttcaatatta ttttgttggc
    10321 gaatttcctc atcatgaata gcctgccaaa gttcttggtc aaattcttta aaattgtctt
    10381 tatcaaaaat cattttttac tccttaagct cgttataact tttaaataat ggtttcaatc
    10441 attatattta tcacattttt actattagtt gcaagatgat taaattaatt ttcaaaaatt
    10501 aattcaggta tcgtagccgt tagtacctct tgcgtgattg caccttgcct taaaatctta
    10561 actctatcac cagacaaatc aataatagta gaatccacac ctgtcaatgc tttatcatct
    10621 tcaataccta aaacttgatg attaaactgt ttttgaattt ctgagaatac acgaccacta
    10681 tcacatccag aaatattagc agaaggacca atcaatggac catggttagc aatcatatca
    10741 agggttacag ggtgcttagg catccgaaat cctacgcttg ataacccaga attgatccaa
    10801 tccggaactt cttgtgaagc ttccaaaata atggttagcg gtcctggtaa aaaagcatcg
    10861 tacaattgct tcaaataggt cggttgattt ttagaatatt ttaaaatagt ctcaaaatca
    10921 catatattta agttcatagc cttatcacga ggtctttttt taagctcata gacgtggttc
    10981 acagcttctt cactcaaagc tttggcaaag attccgtaga ccgtttctgt tggcataata
    11041 actgcgccat gatcctctaa aatctgtccc aaatcattca ttatctaata ccaccattct
    11101 atctttacca aatatatctt tcaaaactct gcaacgattg tttggaaaat atcttttcaa
    11161 taaacttcgt aaatcatcac cttgtttgta acctatttca aaatataatt tacctctagg
    11221 ttgcaaatat tctcttgagt tttcaatgat tttacgataa atagctaatc cttcttcgtc
    11281 tgcaaaaagg gcactatgag gctctgaagc tagcacattc ttccccacct cgtctttgtc
    11341 attgtaagaa atatagggag gattagacac aataatatca aatttatctg aaatgttact
    11401 aaagacatct gattctataa aagtcacttc cgcatcacaa ttattagcat tttcctttgc
    11461 caagtctaat gcagatacag aaatgtcact agctagaaca tcccaagagg ggcgttcttt
    11521 ttttagagaa atggcaattg ctccactacc tgtaccaata tctaaaacag aacaatcttc
    11581 tactttattc tctgataaga ttagatcaac caactcctct gtctcaggcc tcggaattaa
    11641 aactcgttca tcaacaaaga aaataagatc tctaaagtaa gctttacctg taatatactg
    11701 cggtgatcta tgttttttaa gctgttgaaa aatattttcc attaattgct gatcagtatc
    11761 actcacacta ctattttgat ttaaaaggta atcagttgtt gaccattgtt ttaggtcttt
    11821 taatacataa ataaaatttt cgacttcttc accacaagcc tctaacaact gttcataatg
    11881 cttgattaac tgcgcataat tcattattta tttaaggctt ctaacttttg agtctgatca
    11941 tacataacaa gtgcatcaat aacttcatcc atttttccag ataaaatagt atctaatttt
    12001 tgaagtgtca aaccaatacg atgatctgtt acacgatttt gtgggaaatt ataagtgcga
    12061 atacgttctg aacgatcacc agtaccaact gttgatttac gttcagcatc ttgttcatct
    12121 tgagcaattt gcgcaaaatg gtcagcaaca cgagcacgaa tgattttcat cgctttatca
    12181 cggttttttt gctgagtacg ctcttcttgc atttcaactt tgattcctgt aggaatgtgt
    12241 accatacgaa ctgcagtagc aactttattg acgttctgac caccagcacc agacgcgtgg
    12301 taaatatcga cgcgaagatc tttttgatca atttcgtatt ctacttcttc aacttctggc
    12361 attaccaaaa cagttgcagt tgaagtatga acacgaccct gactctctgt caccggaaca
    12421 cgttgaacac gatgagctcc tgattcatat ttaagcttag aataaactga ctgaccagaa
    12481 accatagcaa ccacttcttt aataccacca acaccattaa cggatgattc cataacttca
    12541 aaacgccatc cttgagtttc agcatatttt tgatacattg ttaaaagatc accagcaaaa
    12601 agcgctgctt catcgccacc agcagcacca cgaatttcta aaataatgtt tttatcatcg
    12661 ttaggatctt taggtaatag aaggattttt aatctttctt catactcttc tttagctgct
    12721 tttgattctt taagttcttc tttagccatt tcttccaatt cagcatcacc agaagcatcc
    12781 ttgatcattt cttcagcatc tgaaatattt tgaatgactt gcttatattc tctataagct
    12841 gtgacagttt ctcgagtatt agcttcttca cgtgataatt ccataaatcg ctttgtatct
    12901 gaaacaacat ctggatcact aagtaactca cctaactctt cataacgatc ttcaactgcc
    12961 tgcaattgat cataaatatt catttttctt tgtatctcct ttaaaaccga ttatctttta
    13021 aatatctgga ttaaaataat gttttcgaca aactgggata tatgtttcat taccaccaat
    13081 ttgtatctga tcaccttcat agacaggttt tccgttttct gtccttaaaa ccatggttgc
    13141 tttcttacta cagtattggc aaattgtttt gatttcatca atcttatcgg ccaataatag
    13201 caaatgtttt gaaccttcaa aaagattgtt ttggaaatca tttttcaaac caaaagccat
    13261 gactggtaca tctaagtcat ctactactct agctaaatcg tagacgtttt tcttactcaa
    13321 aaattgacat tcatcaatta aaacacaata aggcttttgt ggtaaattct gaatatagga
    13381 gaaaatgtcc atatcatctg aaattggaac tgcttcacga cgcattccaa tacgacttga
    13441 aacgactcca aattcatctc gagtgtccaa ggcgcttgtc ataataacca caggttttcc
    13501 ttgttcttca tagttatgag caacttttaa aatttcaatg gttttaccag aattcattgt
    13561 cccatattta taatagagtt gagccaaagc ggaaaatcct actttctata gtattctacc
    13621 tattttacca caaaggctga agttttccca gccattgaaa acagtgtgta ctttttcaca
    13681 acattatgtt acaatgatgc ccaataacat aaaggagtaa tattatgcca tttgtaaaaa
    13741 ttgacttgtt tgagggacgc tcacaagaac aaaaaaatga attagctcgc gaggttactg
    13801 aagtagtatc acgcattgca aaagcaccta aagaaaatat ccatgttttt atcaacgata
    13861 tgcctgaagg aacctactat cctcaaggtg aacttaagaa aaaataagaa atagtaaaag
    13921 aggttaccca ggtaacctct tttgatagtc tttgcaagca tatttcttga caaattgaaa
    13981 atatttgaaa ataagtattg attttcaagt tagaaaacgg tatcattaga atatattgct
    14041 ttgggggtgg aaaatttgca actttctcat agtttaagac tcatgggaac gactatcgat
    14101 attcaaatta attctaaaaa tgctcaaaaa caaatccaag aggtcataga actcttggag
    14161 ctctacaaaa atcgctttag tgcaaatgac tttaattcag agttaatggc tataaataac
    14221 aacgctggta tcaagcctat tcaagtacat ccagacctct ttgaattaat cacaattggg
    14281 aaagaacaca gcttagctag gcctagtaac ctaaatattg ctattggtcc tttagtgcaa
    14341 acttggagaa ttggattttc agatgctaaa ctacctagcc ctagtgaagt atcagaagct
    14401 atgattttat ctgacccaac tcatatactt ctagattcaa aaaatcaatc tgttttttta
    14461 aatcagatag gaatgaaaat tgatttgggt gcacttgcaa aaggatatat tgctgacaaa
    14521 attatgacct atctcaaaaa tgagatgatt gattctgcta tcattaattt aggcggaaac
    14581 gttttagttc acggagataa tcctaatcgt tcagaagggt attgggttat cggcattcaa
    14641 catccgaaaa agaaacgggg acaaaatatt ggagctgtca aaattaaaaa ccaatctgtt
    14701 gtaacctcag gtacttatga acgccgtctg attattgacg ataaggaata ccatcatatt
    14761 tttgatcgac agactggtta tccgatacaa acagagatgg ctagcatttc tattgtttct
    14821 aaacaatccg tcgactgtga aatctggacc acaaggttgt ttggcttatc cattaaagag
    14881 gctcttgata ttttaaacgc tgtttcctac attgaaggca ttatcatcac taaagatgac
    14941 cgtatctatt tatctgatgg cttaaaacat cattttcaac tattttatca ttaagaaaga
    15001 ggatttctaa tatgaaactt attggcatcg ttggaactaa ctcaaataaa tcaacaaatc
    15061 gtcaattatt acaatacatg caacaacact ttgctgacaa agcagaaatt gaactcatcg
    15121 aagttaaaga ccttccgtta tttaataaac ctgctgataa aaatgttccc caagtaattt
    15181 tagatattgc tgctaaaatt gaagaagctg atggtgttat tattggtacg ccagaatatg
    15241 atcattctat accttcggcc ttaatgagtg ttttagcttg gctatcatat ggtatttacc
    15301 cacttctcaa caaacccgtt atgattactg gtgcttcata tggaacactt ggatcatcac
    15361 gtgcacaatt acaattacgt caaatactta acgctcctga actaaaagca agtgttttac
    15421 ctgacgaatt cttgttatca cactctctcc aagcctttga taaagatggc aacctacacg
    15481 atatcgaaac aagtcagaag ctagatgcta tctttgatga ttttcgtctt tttgttaaga
    15541 ttactggtaa attatcaaat gctcgtgatt tacttcaaaa agaagcagaa aattttgact
    15601 gggaaagttt ataagagtat agaagggaat aactaagatg aaatttgtag gtattgttgg
    15661 ctcaaatgca gagcaatcat ataatcgtat gttgcttgaa tttattcgca agaattttaa
    15721 aaccaaattt gaattggaag tccttgaaat cgatgatatt ccaatgttta atcaagtcca
    15781 aaattgggaa gaaagtttcc aacttcgtct tcttaataat aagatcacac gagctgatgg
    15841 cgttattatt gccactcctg aacataatca tactattacg gccgctctta aaagtgtgct
    15901 tgagtggctc tcatttgctg tgcatccact cgaaaataaa cctgttatga ttgtcggtgc
    15961 ttcatattat gatcaaggca catcacgtgc tcaaatacac cttcgtaaaa tccttgatgc
    16021 tcctggcgtt aatgcttaca cacttccagg aaatgaattc ttattaggaa aagctaaaga
    16081 agcctttgat gataatggaa acattatcaa tcctgggact gttcaatttt tagaaacttg
    16141 tcttgataat tttatcaaat atgtaggagt tgtatcatca ttgagaaaac ctaaagctat
    16201 tgaatctgaa gacctctact gtcagggaca aatcgataca accattacta gtatcgatcc
    16261 agatgatcct gattggttag acaaggcagc cgagaaagtt aacgccgttg aaggggatgc
    16321 ttacgttaaa ctagacaatg gtttacttac tgttaaccaa cttaatatgt ttttaaatgc
    16381 tatgccattt gagctaacat atgcagatga taataatcaa tttctatatt acaataataa
    16441 tcatccagat ccagatacaa tgttaggaaa acgtgttcgt gaacaagtag gaaaccgtat
    16501 ggccacagtt catggtagtt taccaccggc acgaatgaaa aatgttgaat gggttatcgg
    16561 tactcttcgt aatggaaatc aagaatatgt acgtacagtc gttcctggta cacctccaga
    16621 agttatcaat actcacaact atcaagctat gtactaccca gacggatctt atgctggtat
    16681 taatgaaatt atctttaact ttaaaccgtg gttagattgg tacctccaaa ctactggaca
    16741 acgtttggtc ggtggaacta ctgctgctcc tggagcttca gcagttgacg ctacttctag
    16801 cgcttcaact agcacagagg atgctcccgt tgatcaagcc acagatgcca cttctggtgc
    16861 ctctgaacat taatctcctc gcttttgagg atctgatacc ttaaaaagat atcaagatcc
    16921 tcctttttaa tatttaatca agttaataaa atcacataaa aggaatatac tatgtcactt
    16981 tttcaagaaa aaattgctta caattgcgct aaaaaggaag cgctttataa agagagttta
    17041 ggacgctacg ccttgagatc aatgctagca ggggcttatt tgacaatgag tactgctgcc
    17101 ggtatcgtcg cagctgatac tattggtaaa atttctcctg ctctatcagg ttttgtattt
    17161 gctttcatct ttagttttgg acttatttat gttttaatat ttaatggtga attggcgaca
    17221 tctaatatgc tttatctcac tgcaggagcc tataataaaa atatctcttg gaaaaaagcc
    17281 ataacaattt taatttattg tacttttttc aacctcgttg gtgcttgtat attagcttgg
    17341 ttgtttaatc aatcgtactc attccagcat ttaacaaatg atagcttttt agggcatgtc
    17401 gttgctaaaa agttaggaaa acctagttca ggagctttcc tagaaggtat tattgctaat
    17461 atgtttgtga atttagctat ccttgcttac atgttattaa aagaagaatc ggcaaaaatg
    17521 acggttattt tatctgctat ttttatgttt gtttttctat caaacgaaca cttaattgct
    17581 aattttgcat cctttatgtt agctgctttt agccatatcg aacatattaa aggttttact
    17641 cttctaaacg ttatccgcca atggacactt gtattttttg gtaactggat aggagggggc
    17701 gttttcatcg gcctagccta tgcttggtta aataaaacaa aaacttctca ccatgaataa
    17761 aagaaaaact gagtaattta ttactcagtt tttcttttat attctcatca tgaaatcaat
    17821 aactaaaaat taactttttt gataaataaa ggaaagtatc atattaattt tttaggcaat
    17881 ccattcaaaa tgatatttaa aacaacagct gtcagggttg agataacaat accatttgtc
    17941 aaaaacattt gtaaagtatt aggaagacta atgaagagat ttgtctcatt aaaacctact
    18001 cctgctgcaa ttgatacggc tgcaatgata aagttatgct cattatgctc aaaatcaacc
    18061 tgattaagca ttttcatccc ttgaagtgct accataccaa ataaaactaa catagcacca
    18121 ccaagaactg gacttggaat catttgagcc attgcgccaa atttaggtaa caagccaagt
    18181 ataactaaga agagagctgt aaaatatatc ggcttacgcg tacgtatccc agatatctgt
    18241 accagtccca cattttgaga aaaaccagta tatggaaagg tattaaataa gccgctaagt
    18301 aatactgcca atccttctga acggtaacca ttacgaagtc tcttactgtc taatttatcg
    18361 tttgtaatat ctgaaagcgc aaggtaaaca cctgttgatt ctaccataga aactgttgca
    18421 ataatacaca tcattaaaat agaagtaaat tcaaatctag gagctccaaa ataaaatggc
    18481 ttcggaatat gtacaagtgg tgcttctgct accacagaag catcaactaa gcccataaat
    18541 gctgcaagaa ttgtacctga aatgagtcca ataaggattg aaattgactt caaaaagcct
    18601 tttgcgaaaa tattaattaa taaaacaaca ccaatcgtta ccagtgataa agttaaagat
    18661 tgtaagcttg gttcttttgc attatcaccc atatttccca tcgcaactgg tattaatgtc
    18721 aaaccaattg tagtaataac tgatcctgtt acaattggag gaaagaaatt tgcaacttta
    18781 gaaaaaatgc cagcaactaa aacgacatat attcctgaag caattaaagc tccaaacata
    18841 tagccagaac cttgttgtgc accaataatt gataaaggag ctacagactg aaaggcacac
    18901 cctaatacaa ctggaagacc cactccaaag tgcttactta atcgtaattg taataaggtg
    18961 gcaatcccac acataaaaat atctgtcgca ataagataag ttagttgctt agcattataa
    19021 ccaagagcac tagcaatcat gataggtaca agaatggatc ctgcatacat agctaacagg
    19081 tgttgcaagc ctagaagtgc tgcttgcgaa ttactttgct cattaaattt cattaagcat
    19141 cagcctccgc aaaaacaact cgaccatttt caaacgcttt aatacgagca agagaagtaa
    19201 ccggaacacc tgttttttct aacaaatcac gcccatcttg gaaagatttt tcaataacga
    19261 ttccgatacc agcaacctta gctccagctt gaccaataat ttcaagtaat cctttagccg
    19321 cttgaccgtt tgctaaaaag tcatcaatga tgagtacagt atcatcgtta gataaaaagc
    19381 gactcacaat agaaacttga ctcgtaactt gctttgtaaa agaatacact tcagcagtta
    19441 agataccttc agtcatagta atgttcttag cttttttagc aaatatcatt ggtacgccca
    19501 atgcttgagc tgcgtacact gctggcgcaa ttccagatgc ttcaattgta acaaccttcg
    19561 taatgccggc ttctttatat ttatcagcaa aaactttacc tatttcctgc attaactcaa
    19621 aatttacctg atgagtcaaa aaagaatcaa cttttaaaat gttctcacct aaaacatcac
    19681 catctttaag aatacgttct tccaataact tcataataac cccttcactc tttagaaaat
    19741 aaaaaaataa cttacctcaa tcagaggaca caaatctcct ctaatttatc gcttcattaa
    19801 catgaaccaa caggcaagct atttattagc ttacttattg ttagacatta cggtgtcttg
    19861 tagagacgct aagccaatcc tcagcataaa taagtaaatc atatatttaa ttgttatgta
    19921 tgatagataa ctattgtaac atttttaatt aagatttcaa gatttaattt aaatggaatc
    19981 accattccaa atagaatttt taacaataac gtaatcaacg tgtctcaagc tatccaattc
    20041 ttttccacct gcgtaagaaa tagaactttg caaatcttgt tgcatttctg tcaaagtatc
    20101 ctctagtcgc cctttaacag gtaatagaat tttcttacct tcaacatttt tatgttctcc
    20161 cttttgatat tcagaagctg agccatagta ttctttaaat tgctgtcctt ctacttccac
    20221 aagttttcca ggactttcaa ggtgaccagc aaacagcgaa ccaatcatta ccatactagc
    20281 accaaagcgg atagattttg caatatcccc atgtgttcga ataccaccat cagcaataat
    20341 tggcttgcga gcagctttag aacaccaacg taaggcagct aattgccaac ctccagtacc
    20401 aaaacctgtc ttgactttag taatacaaac tttaccaggt ccaataccaa ctttagttgc
    20461 atctgcacct gcattttcca attctctaac agcttcaggc gttcctacat ttccagcaat
    20521 aacgaaagtt cctggaagct cttgtttaat gtgttggatc atctctataa cactgttaga
    20581 atggccatgt gcaatatcaa tagtgataaa ctcaggagca tcttctttca gacttgtcac
    20641 aaaatcatat tcatagtctt tgaccccaac cgaaatcgaa gcaattaagc ctttatcatg
    20701 catacgcttg ataaaaggtc ttcttgcttc ttcattaaaa cggtgcataa tataaaaata
    20761 accttcacac gctaatgttt cagctacctc ttcatcaata atagtctgca tattagcagg
    20821 gattactgga agtttaaagg tatagtttcc aagtttgaca cttgtgtcag cttgtgagcg
    20881 gctagatata atacacttgt ttggtattaa ttgaatatct tcataatcaa aaacaggaat
    20941 atcgttaaac ataatggttc tcctaatgag taaaatgacc ttcctataac tttgtataac
    21001 aaagcatcgc taggtaaaaa catacctttc ctttttaaat tatatacttt atttattttt
    21061 agtcaatttt tgatcaattt atttgaataa tgttcgtttt tttcaaaata aatgtaaaat
    21121 cctatagagc agtactctat aggattttac atttattata ttgcttttct ttttcgaaga
    21181 taagttaatc ctacaatagc taagataaca acaacgataa cgaatagaac tgcaaattga
    21241 ctaccaatta tagctccatt tttcggaata gctacttgtc cgatggtaaa aatttgtgag
    21301 gctactgcgg ttgcaaaagc tcccgcaaat tgttgtagtg tatttaagat actattacca
    21361 tccgctgact gtgctggctt tagttttgtt aaagctgtcg taaccatatt acttgctcca
    21421 atccccatac caattttagt aagaatatca aaggctaaaa gtactacaac aaacttcgca
    21481 ggaatgaata tagctgtacc aattaaacct atcaaagaaa gggagatacc tatcataatt
    21541 ggcttaaatg caccaatttt atccaggaga taacctgcaa aaggggataa aaaaactact
    21601 gctattgcac ctggaaacat aaacaaacca gctttggttg aagtttgatg taaaacaagt
    21661 tgcaagaggt taggtaataa gaacgaaagt gctagaggaa tcatttgaaa agccaggacg
    21721 ccatacaata agattgagaa atctttatct ttaaaaacat tcaaattcaa aaattctagc
    21781 tgtttattta aatagtaaaa aataacaaaa ctaacaatag ctactaataa gtaaaaaata
    21841 gacatctttt caatagctaa aagaagggtt gccaagccaa tacctaaact gacaaaggca
    21901 ggaaaattga gtttatcatt aattcttact tgacgtttgg gaatagaagc caaacctata
    21961 aaggtagata tgataagtat tggtgccaga agcataaaaa tcatcttcca tcctaacatt
    22021 cctgaaatga caccaccata agtaggtcca actgccggtg ctaaaagtgt gaccatagca
    22081 gctactccca tcattagtcc tctacgctcc atcggaattt gtgtaagaat gatatgaaac
    22141 aataaaggga gggctaatcc tgtcccaaca ccttgcaaga ctctccctac taacaatata
    22201 gctaaattag gggcaaagct atcaattaaa acaccaaata aaaaaagcac atttgccaat
    22261 gtaaaaattt gtctctctga taaattttta atcataaatg ctgacaaagg cacagtcaca
    22321 gcaacagcta acaaatttcc tgttgtcacc cattgaataa cagcggggtt aaccccaaat
    22381 tctttcatca aaagcggaaa agtgacattc atagatgttt caataagaat ccctgaaaaa
    22441 cctaatatag ccgttgtaat tatggcaggc ataatacgcc atcccttttt agtcattaaa
    22501 atctccttac tttgattatt atcataaaat agagcacccc gctaaatcaa ggtgctccat
    22561 ttaattcatt cgaattaaaa ttaaattgtt tatattagtt gtcaaaggac aatcaacagc
    22621 tcttattata tcaaataaag ctaagaaaaa caaagagttt tttgaatatt aatgccttca
    22681 ctttcttatc tattctctac ccatcaatca ccacttcaga gaacataaca attgcaattt
    22741 actcttaata ccgttatcat gttcatatgc taatacccaa gaaaatagtg gcttttgcct
    22801 caaatgaatc tgaaaataat ttctttatta cgttaaagac ttactttaat tatttgtttt
    22861 ctattcaaat cattacagat atttcaactc tcaatcatgc agattttgac ggtagctttg
    22921 cattccatga tatagaaact agcatacctc atctggtaat tgactccaac tacttagcga
    22981 ttagtcagac taactcaaaa acagaggcta acgatattaa aacgttctcg gaactctcca
    23041 aaacaatgac cgagttttac tatatgctaa attttgactt atttaaccat ctcccatatc
    23101 gttttagatt gcataataag gacggtcaaa caatttattc gaaccataaa cctgaagatc
    23161 cttttgatat ttatcctgaa gaagaatatc ctatagacaa atgggttcaa aacagtctaa
    23221 tagagaaaaa agcgaaagag ctacatttac tcttacctgg cgcatcacaa gattatattc
    23281 tagtacaatc ttataaacgc ttagaaaatg atagtgggca actagttggt tatattgaac
    23341 atgtccataa tattaaacca ctattagagg ggtatttaaa agaatccggt caagctatcg
    23401 ttggatggtc tgatgtcacg tctggtgctt ctatctctaa cgatgatttt gaactataat
    23461 ccactcgtca ctctaaaaag aaaactaata actaaaaggc actggtttaa ttccagcgcc
    23521 ttttattaat caatctccag ttgttgtaag ttcaattttt tctttaattg gtagacctcg
    23581 attagctgta aaaatcaaag gaactacttc tgttgtaact tcggaaaatt gtagaccaaa
    23641 ccatcgtatt ggtgttgctg tccaatgctt tacagatgat ttcattagca taacaaatcg
    23701 ttcaccaggt tttaggtttt gactgcttga aaaacgttca tcaaggacaa taaacctaaa
    23761 atctcctact ttactatcag gtctaacgct gtatgtcttt ccttgtttag gcagacgtcc
    23821 actttcaagt aattcttcta caatcgtacg aagatagcgt gacacagtct gacgcatctt
    23881 aaatccaagg taaagttcta ccttcacaat gaagtcggtt cccatcatgt caactttata
    23941 ctcagctgta taaggttcat cagtgacctt aatgtttaca aaccaataaa cttgtgcttt
    24001 tttagggcgc ttatcaagga tagaatacat gatagactta tcaatcatgt ctccttccat
    24061 acgatttgtc aaataaacaa cgttagtatg ataaatcgga taacgatgat catgtctaag
    24121 tttatctaat tgtccgatgt aatcttttaa atccaacaac ttaacataac gcgaaacaat
    24181 tttactacct ttataccaga tagtcatgat gaatataatt gctactgcta taataacaac
    24241 aacatagcct ccatgcataa atttaacagc tgaagcaagg aagaagattc cctctaaaat
    24301 accaaagaaa atcatcatca ataagactaa acctcttttt actccttttt gaattaaaaa
    24361 gaaagacagt aaaatagttg tcattagcat cgtaattgtt atcgctaatc catatgctgc
    24421 ttccatgtgc gctgaagttt taaaaagcaa aacaatagag gttgtaatgg caaataagaa
    24481 ccaattaata acaggtatgt aggtttgacc aatattgtca ccaggataag ttgaacgaaa
    24541 ttgtgggaat atttttagtc gcatagcttc agaaactaag gtaaatgatc cagaaatcag
    24601 tgcctgtgaa gcgatgatag ctgccaaagt agctaaaata acgacatgca ttgtaaattg
    24661 cgaaggaata ctagcaaaaa agggattcaa ttcatttcct gcgttcttat tagctaaaat
    24721 ccatgcccct tgcccacaat aagaaagtat aatggcaacc ttaacaaacg gccatgaaac
    24781 atgtatattt ccacgcccaa catgacctaa gtcagagtat agtgcttctg ctcccgttgt
    24841 cgccaggaaa atagatccta aaataaaaat acctttatga ttctctggac taaataacaa
    24901 tttcaaaccg tagtatggat taattgcttt gaaaacttct ggatgggcaa aactattaag
    24961 gagaccgcta atacctaaaa aggcaaacca tataaacata attggcccaa ataatttccc
    25021 aataacaccc gttccaaacc tttggatggc aaaaagtaac agtaaaataa acaaggtcgc
    25081 aaaaataaca ttactctgat tctggaaaat atgctgcaaa ctaggaacta cttttaatcc
    25141 ttcaacggct gaagttacgg ttacagctgg cgtcaaagct ccatctgaca acaaggttgc
    25201 acctccaata acagccggaa caattaacca aggtgtcatt tttctaacaa gggtatataa
    25261 agaaaaaata ccaccttcgt ggtgattatc cgcctttaaa gctacaagca catacttgat
    25321 agttgtaata agtgtcaagg tccatatgat taaagatata gaacctaaga taaacgattc
    25381 tgtgacacta gaaataccac cttggttttc aaccaatgat tgcatcgtat agagtgggct
    25441 tgtaccaata tctccataaa caatgcctaa agcaataata aatcctgctt ttgatgcttt
    25501 atcaaaagaa gaatgattga catgttgcat ttctgccaac cctccgatta aataatatta
    25561 gtcaaaaaaa tggctgaact cattatagca gattaagtta aaaaaacaac acttatctat
    25621 aaaaagacca gaaattatta aagcgtttcc tggtctttac tattttaatc acgataaaca
    25681 ggttgaggtc cataagtttg actgactggc attatttcta tcgattaata ttaatgtgtt
    25741 ctggttggct agcaacccat gacacggtat tagcaatatc ctctgcttga attgcatgcg
    25801 caccttcata aagtttttca actcttttat gatctccttt gaaacgtaca gttgaaaatt
    25861 ctgtcccttc acataaaccg ggttctaggt tagtaactct gattttagtt ccagctaaat
    25921 cggcacgcaa gttgagagca aattgtttta caaaagcttt tgatgcgcca tagacattag
    25981 ctcctggata aggaattgtt cctgcagttg atcccaaatt gataattaac cctctattaa
    26041 cttctaccat tttcggtaaa atgcatcgtg tcaagtaaat taatcctaca acgttagtat
    26101 taatcatagt catccaatct tcaaaatcag cttcatatga tttatccaat ccaagtgcta
    26161 aacctgcatt attaaccaac aaatcaattg attgcaaatt tttaggcaga ctttctaaag
    26221 ctttgtcaac cgattctcta cttgtaatat ccatttaaag tggataaaag taaccctctg
    26281 aaaacttttc tccaaggcat tttaatttat ccattcgacg agcacaacca ataacgctat
    26341 aaccatctga aactaattta gtaacaacag ctgcaccaaa accagcagat gcccccgtca
    26401 caagcgcaac tttacgtgat atcataaata ctcctcctct agtatataga aaaacgagct
    26461 aggaaaaatc cttctcgttt tataacttat tttgatttct catcaatagc ttgagcagct
    26521 gtaatgatag caagtttgta aatatcttca gcacttgatc cacgtgataa atcatttact
    26581 ggagcattca atccttgtaa gatcggtccg attgcttcaa acatacccaa acgttgtgca
    26641 attttatagc caatatttcc tgattgaagg tctgggaaaa taaaaacatt tgcttttcca
    26701 gcaacatcac tattaggagc tttaatttct gcagtttccg gaacaaaagc tgcatcaaat
    26761 tgtaattctc catcaacagc taattcggga gataaatctt tggcaatttt agctgcttct
    26821 tgaacttttt cagcttgagg cgcttttgca cttccttttg ttgaaaaact aagcatagca
    26881 atcttgggat taatatcaaa tattttggct gtatccgcag tatttactgc aatttctgct
    26941 agctcttgtg cattaggatc aatgttaata gcacagtcag caaaaatata acgttcttgt
    27001 gtattttcac ggttcatcaa gaatacacct gaagtgcgtg aaattcctgg ttttgtttta
    27061 ataatttgaa gagcaggacg tacagtatca gctgttgaat gaatggcccc tgataccata
    27121 ccatctgcca aaccaagttt tactaacata accccaaaat agttaacatc cttcaaaaga
    27181 cgatctgcat cttccatagt agctttgcct ttacgaatct caacaaaagc ttctttcatc
    27241 tcttcaaatt tttcgtattg ttcagggtca ataacgctat aatcttgatc aacaaaacca
    27301 agtcttgcca aagaatcgtg aacttcgtca gccttaccta aaataacagg atccacaaga
    27361 ccttcaaatt tcaaacgagc tgctgcacgt aatacacgtt catcgcttcc ctcagggaaa
    27421 acaatcttaa cataatcact tgcaactttt tctcttaaac caccaaataa ggaacgcata
    27481 tatataaaac cttctatttc taaattaatg taattccatg ataacgctgt caaagtcatc
    27541 tgtcaagtct agcgtctgtt tatttatttt tcccataaaa gggtcgtaaa aagacaaaga
    27601 atgacaatgc aatgcttgcc tgtttattcc tagatccata cgtcctccgt acaaatcatc
    27661 tcccaaaaga ggaaagccga tatgagcaaa gtgaactcta atttgatgag tacgccctgt
    27721 atgtaatttg atatccacta atctaacatc accataacga gcaactactt gataactagt
    27781 atgtgcatat ttgcctgatt catggactcg cctcgtaata atactatcaa catctcgtgc
    27841 aataggagct ataatatcac cactatctgc taaatcacca ctccctgata ctaatgcata
    27901 ataccttttt tcaattgctt tagcctgaag ttgtttatct agacgagcat gggcataacc
    27961 atgcttggcg aataacatta atccacttgt atctcgatcc aaccgggtca caatatggac
    28021 ctgctgattg gcatagttat tagacacata atagtgtttt ataaaattag caattgtatt
    28081 tgaatgaata gagctaggaa tactagcgaa accaaaaggt ttatttaaaa tcaaaaaatg
    28141 atcatcctca taaacaatat ctaaatcgtg aggtactggt tccaaagttt cgtgagaagg
    28201 ctcgtcagga atatctatcg taacaacatc gccaatctct aataagaaaa tagcgttctg
    28261 ctcttcacca ttgacaaaaa tcttaccacc tctgtactta atctttgcta ataaccccct
    28321 tgaaacatca tgacttttca aaagtgtttt aactttgcac ctttcttctg ctatgtattc
    28381 aaatttcaat gttcaggttc tccgataaaa gcatcgttaa cacgttccca aaagctagtg
    28441 tgacttggag ttgaaacaaa gttaatactt ttttcgtcaa tactatattc tattttggta
    28501 acatttttgt aatggaccgt tttgttatct atagaaattg tatagacacc aactcgttta
    28561 ggaactatct ctatagcatc ttttttgggg atgataaccg atgaccccaa ggtacgatag
    28621 acgcgattat taagactaga aatttctgtt agttgcaagg cttctatcgt tgggtggagt
    28681 actgcccccc ctagtgattt attgtaagca gtactaccgg ttggtgtaga aactaaaatg
    28741 ccatctcccc taaacctttc aaataccact tgattaataa caacatccgc taccattgtt
    28801 ttttcaatac gcttaatagt agactcattt aaagcacgcg cacgaataac tcgaccatct
    28861 tcaagggtaa tagttacttt caaaatagga taggagattt gttcaccttt atcatttttc
    28921 aaattattaa ttaatgtgtc aacttcaaaa tctcgatagt ctgtataaaa ccctaaatgt
    28981 cctgtatgaa caccgacaaa ccttacttta tccagttgtt tttcgtacat atggaaggct
    29041 gacaaaagca taccatctcc accaatagaa atgacaatat caggatcctt ttttgataaa
    29101 taaaaatcag gatcatgttt aaaagcagca aagagctttg aggctacacg cttgctttga
    29161 tattttccgt tagcaattat agcaacacgt gttgctctat ctgtaaaatt catctgtgtc
    29221 atcactattt cctacaccat cgctcaattt tcgatttaaa ggatcaaata aaagctgtgc
    29281 ttcacgaata tcttcgcgaa tcttacgcat ttcttcatcc aattctaaag cgatttttgc
    29341 tgttttctcc agtctttgtt taatttcttc agggaagtcc ccctgatact tgtaatttaa
    29401 tgaatgttcg atggttgccc aaaaattcat agccagagtt ctaatttgta tttctgctaa
    29461 aactttcttt tgtccatcta ttgtgtctac aggatactct acaacgacat gatatgaacg
    29521 atatccacta gatttcatat gggtaatata gtctcgctcc tgaaccactg tcatgtcatg
    29581 acgttttctc aacaaggcaa ggacttcatc aacgtcatct acaaattgta ccatgatacg
    29641 aagacctgca atatcttgca agtcctgagc aagattttct tctgaaatac cacgtaaaac
    29701 catcttttct tgaatacttt caaccgactt tacgcgtcca gtaacaaatt ctattggaga
    29761 gtgacgattt tgctttctaa attgtttacg aataccacga agctttattt ttaattcacc
    29821 aaccgtttga atataagggt ctagaaatgt ttcccaatcc attgacattc taatcctcca
    29881 aaatatttaa tgtactctct tgtaaaataa actttttttc tataaaatta acacaacaat
    29941 taatattata ccatatttag gaaaaggaag tgataagatg acacacctag aaattgaata
    30001 caaaactcta ttgaataaag acgaatttaa tcgtctaaca agcttatttt ctcatgttca
    30061 acctattact caaaccaatt actattttga cacagaaaca tttgaaatga aagcacaccg
    30121 aatgtcactt cgcattagaa cgttacctaa ccgtgccgaa ctaacactaa aaatccctag
    30181 agaagtagga aatttagagc acaatcatga ccttacttta gaagaagcta aatatatcgt
    30241 taaaaatggc cagttccctg aagatactga gattgcttct ttaatccttg aaaagggagt
    30301 tgatccaacc aaattagctg tctttggaca acttacaaca acaagacgag agatggaaac
    30361 ttcaatcggc ttaatggctc tagacagcaa tatatatgct gatattaaag attatgaact
    30421 tgagttagaa gtcaaacaac ctaaacaagg gaaaagagat ttcgaccagt ttttaaaaga
    30481 aaataatatt aattttaaat acgccaaaag taaggtggct agattctctg ctactttaaa
    30541 aaataactaa cgttgagaga ttggattttc caatctcttt tataattctc atttaagcaa
    30601 gaacttaaaa tctcatacta tctctcctca acattgttat tgcaagcatt ttcatgtttc
    30661 ttatcgtgaa ttttttctat ttttttggta aactattagt atcaatctaa aggagtcatc
    30721 tatctcatgg cagaacaata cgccgataaa caaatcaaac ttttctcatt aaccgctaat
    30781 agagaaattg ctgaaaaaat ttcccaagct tcaggaattc ctttgggtaa gatgtcatca
    30841 cgtcaattct ctgatggcga aattatgatt aatattgagg aaacggtgcg cggtgacgat
    30901 atctatatca tccaatctac aagcttccca gtcaatgata atctctggga attactcatt
    30961 atgattgacg cttgcaaacg tgcaagtgct aatactgtta acatcgtagt cccttatttt
    31021 ggatactctc gtcaggaccg catcgctgca tctcgtgaac ctattactgc aaaattggtc
    31081 gctaatatgt tagttaaagc tggagttgat cgagttctta ccctcgactt acatgcagtg
    31141 caagtacaag ggttcttcga tatcccagtt gacaatcttt ttactgtccc actatttgct
    31201 gaacactata atcaactcgg tctctctggt gaagatgtcg tcgttgttag tcctaaaaat
    31261 tctggaatca agcgtgctcg tagtttggca gaataccttg actctccaat tgctatcatt
    31321 gattatgcac aagatgattc tgaacgtgaa gaaggctata ttattggtga ggtagaaggt
    31381 aagaaagcta ttattattga tgatatttta aatacaggaa aaacctttgc cgaagctgca
    31441 aaaattttag aacgtggtgg agcaactgag atttatgctg ttgctagtca tgggcttttt
    31501 gctggcggcg cagctgatat tttagagtct gcaccaatta gagaaattat cgtaacagac
    31561 tctgttttat ctaaagagcg tattcctagc aatattaaat atctgaccgc tagccatctc
    31621 attgcagacg ctattatccg tatccatgaa agaaaacctt taagtccatt attttcatat
    31681 cgttcagata agaaggatta attgatgatt tatttagaca atgctgctac taccgctcta
    31741 accccatctg ttattgagaa aatgaccaat gtcatgacaa gtaactatgg taatccatct
    31801 agtatacata cctttgggcg tcaagcaaat caacttttac gtgaatgtcg acaaattatt
    31861 gctgaatatc taaatgttaa ttcacgtgaa attattttca cttctggggg aactgagagc
    31921 aacaatacag ctatcaaagg gtatgctctt gcaaatcagc taaaaggtaa acatattatt
    31981 acctctgaaa ttgaacatca ttcagtccta catactatga cttacttatc agagcgattt
    32041 ggttttgata ttacttactt aaaaccaaac catggacaaa ttactgcaaa agacgttcaa
    32101 gaagctttac gagatgatac tattatggta tctctcatgt ttgctaataa tgaaaccggg
    32161 gactttttac caattcaaga gattggtcag cttctcagga accaccaagc tgtttttcac
    32221 gttgatgccg ttcaagtctt tagcaaaatg gaacttgatc ctcattcttt aggaattgac
    32281 tttttagctg cttctgccca taaatttcac ggtccaaaag gtgttgggat actttactgt
    32341 gctccccatc actttgatag tctacttcat ggtggagacc aagaggaaaa aaggcgtgct
    32401 tcaactgaaa atataattgg tattgctgga atgtctcaag ctcttactga tgctacgact
    32461 aacaccctta aaaattggac tcacattagt cagctgagaa cgaccttttt agatgctatt
    32521 tcagaccttg acttctatct taataacggt caagactgct tacctcatgt acttaatata
    32581 ggttttccta gacagaataa tggcttgtta ttgacacagt tagatttagc tggattcgca
    32641 gtttcaacag gttctgcatg tactgcagga acagtcgaac ctagtcatgt cttaacaagc
    32701 ttgtatggag ccaactcacc acgtctaaat gaatcaatac gtattagttt ttcagaacta
    32761 aatacccaag aagaaattct tgaattagct aaaaccttaa gaaaaattat aggagattaa
    32821 tatggcattt gaaaaagaaa taaatcttcc agactgcaat tacatttaca ctatcagccc
    32881 gaacattaaa aaatatacct tacgcgatac tacattcact caaaatcgct taggtaatta
    32941 tgaattagag cgtctcctag aaacagtacc taattctggc gatggctttc ctctaaagat
    33001 tacaatcaat aaagaacttt ctggttttaa gctcttaatt actgataaat ctggccttag
    33061 aatagtaaat atctttaaat ctaccgatca taaaattctt caagacaaat tttatttctt
    33121 aatggacagc ctcgttgagc gtaatatctt tacgaaaact gcaaaataat ttaccgacca
    33181 ctatttttaa cattttagtg gtctttttgc gaaccatttt gttgtcactt tcacaatctt
    33241 tttgtacaat agagatgtga agaattaagt tatataactg attctaaatc gaataataaa
    33301 atttttaaca aggagataat tttgtgatta tggataagtc tattcctaaa gcaactgcta
    33361 aacgtttatc actgtactac cgtattttta aacgttttaa tactgatggc atcgaaaaag
    33421 ctagttccaa acaaattgca gatgccctag gtatcgattc tgctactgtt cgacgtgatt
    33481 tttcttattt tggtgaacta ggacgccgtg gttttggtta tgatgtcaaa aaacttatga
    33541 acttctttgc agaaatattg aacgatcatt ctacaacaaa tgttatgctg gtggggtgtg
    33601 gaaatatcgg tagagctctc ttgcattatc gtttccacga tcgcaataaa atgcaaattt
    33661 caatggcttt tgatttagat agcaatgatt tagttggtaa aacaaccgag gatggaattc
    33721 ctgtctacgg tatttcgact atcaatgacc atttaataga tagtgatatt gaaactgcta
    33781 tcctaacagt acctagtaca gaagcccaag aagttgctga catcttagtc aaagcaggta
    33841 taaaaggcat cttgagtttt tctccagttc atttaacatt accaaaagat atcattgttc
    33901 agtatgtaga tttaacaagc gaattacaaa ctttacttta tttcatgaac cagcagcgat
    33961 aaatagttca ttatttataa aatatctgct tcttctcgaa aactataata tttatttttt
    34021 ccaacgatga tgtgatctag gcagacaatt cccagatgat cacatgatcg ttttattttt
    34081 ttagtgaaac ttaaatcact ttcactggga tttggagaac ctgagggatg attatgtata
    34141 ataatcaaag aagttgccat gtttttacaa gcataatgta gaatttctct tggctctgct
    34201 actgaacgac gtacagtacc aataaaaata gttctctgtt cgataatacg attttgtgta
    34261 tccatataaa tagctactaa atgttcttgt tttttatccc ctaattcgag catcattttc
    34321 ctcgctaatt gttcactact taaaatttgc tctttacgat catattcagc tttgtgaatc
    34381 cgttttgcta gttctagcat agcttttatt tcgacggatt taacctgacc gattccttta
    34441 atggattgca actcctgtaa ggataattga ccaaaatctg ctaaactgct tatgttttct
    34501 aaaatttgcg ttgaaatttc aagaacaggt ttttctttaa tacctgtacg taagagaatg
    34561 gctaataact cctgattact caatctatct gcgcctaaat caactaggcg ttctcttggt
    34621 agtaaagctt ccttttttaa ttcaatatgg tacataaaac acctccagtc ataatattcg
    34681 taaactaatc aaaaactcat gttttaaatc aataaaaaat acttagatga aaaatctaag
    34741 tattttttat ttaacgatag gttcgtgcac gatttctaag cgtttgtcgc ttgataatat
    34801 gatcttttac aatctgatag atagtagctg caacaggaac agcaagtaac atgcctaaga
    34861 tcccccataa agcacctccg atagtaatag ccattaaaac ccacatagaa ggcagtccaa
    34921 tcgatccacc gacaactttc ggataaatga catttccctc aaattgttgt aaaaggatca
    34981 agaaaagaac aaacaagaat gcttcagtaa gcgattcagt agcaattaag atgaaaccga
    35041 ttgtaacacc aatgtaggct cccacaaccg gtattagagc tgtaaaagca actaaaaccc
    35101 caactgttaa agcataagga aattggaaga tcaacatacc aataaccgtc aaacttccta
    35161 aaatcatagc ttctaaagtt tggcttacaa aaaaaccatg gaaacgttga tgaaggatat
    35221 gacgaacgta atggaatgtt ttgcctgttg aacctaaata ggtatcaatt aacaaattaa
    35281 attgacgtcc caactgctcc ttgtttgcca aaacgtaaat tgaaaaaata aaactaacaa
    35341 aaacattcag aagtgttgcc gcaatagagg aaactgaggt tagtaaattt gttaaaacag
    35401 ataaaacttg cttcaaaatc tgctggctat aaccacttaa agtagaaact aagtctttat
    35461 ctgttcccat ataatttaaa gcctcagaaa tttgtttatt ttcattgaga ttattaacca
    35521 atttagcaag tgctccggta tcaataacca ataaagaact caaactagaa atcaaatctg
    35581 gaatgacaat tgaaaatagc cagacaatta aaccaataaa agttgcatag gataaaatca
    35641 tagaaacact acgcttgatt gccattaata gtctagatcc tttaaaaagc tttatatata
    35701 atcgctcata gactgacatt acaatattaa taatataggc aattccagct cccatcaaaa
    35761 aaggaaatat tgccttatga agcgtagtta aaatattaga aacagctccc caataagcct
    35821 gtatagcata gcaaatagca aatgttatga caacataata gacctgccgt ttttcaaatt
    35881 tcatgaaatc tccttattaa tcttaatttc tttcaattat tatacactat tttatgaatg
    35941 agaaaaagag agttaacagt tattagtctg ctctttattc tatttgactg ccattacttg
    36001 caataacctt cttgtaccaa taaaacgatt ttttcttgta acgcttgagt gttccctctc
    36061 ctttattatt gcgatcaaca taaattaaac catagcgttt ttccatttca ccagttcctg
    36121 cagatactaa atcaatacat ccccatgtag tatagccaat tagattaaca ccatctacat
    36181 atatagcatc tctcattgca gcaatgtgct gacggaggta atcaatacga taatcatctt
    36241 ctacgtaacc attctcatca ggaatatcta ctgctccaag tccattttca acaataaaca
    36301 tcggtttttg ataacgatcc cagatagcat ttaaggtaat gcgtaatcca agcggatcaa
    36361 tttgccatcc ccattcagaa gatttcaagt aaggattctt aagagatgcg aaaatatttt
    36421 cttgaacctg ttcactgaca gttggattac ctgaagctac tcggcttgaa tagtaagaaa
    36481 atgaaataaa atcaaccgta tagtctctta acaaagcaag atcttctgct gtcatttgaa
    36541 ttgaaatacc taaatgctca aaatattttt tagcataatt agggtatttg ccacgcgctt
    36601 gcacatcaat aaaaaaataa ttttctctat ttttttgcat cgaagcccaa taatcttgtg
    36661 gatgacacgt atttggatag tattgacctg cagctaacat acatccgatt tgactatttg
    36721 gatcaacttc atgagcaatt tttactgcaa tagccgaagc cactaactca tggtgagctg
    36781 cttgatattt aatttgctct tgattttcac catcttcaaa atataagcca gcgcccataa
    36841 aaggagcgtg taaaatcata ttgatttcgt taaaggtcaa ccaatattta actaacccct
    36901 taaaacgagt aaataaagct cgtacaagac gctcataaaa accaatcatt ttacggctcc
    36961 gccacccacc gtaatgttta ataaggtaga taggacagtc aaaatgtgtg atggttacca
    37021 aaggttcaat accatactta cgacactctt taaaaatatt ttcataaaac cgaagaccag
    37081 cttcatttgg atatagctca tcaccctttg gaaaaatacg cgtccatgct attgacatac
    37141 gataggtttt gaaacccatt tctgcaagta atgcaaggtc ttctttataa tggtgataaa
    37201 aatctattga ttcttttgct ggataaaagt atccttcctc aaaatcaaac attttctttt
    37261 gtcctgaaat aattgcaaat cgatcttctc cagtaggaac aacatctaca tttgctaggc
    37321 cacgtccatc aacattgtaa gctccttcac attgattggc agcagttgcc ccgccccata
    37381 aaaaaccttt tggaaatact tgttttacca tcatttaccc ctcttgtttt ttcttatcat
    37441 aatattaaaa aagaccagtt actgacattg attcgccaaa tactggtact atccgtgaca
    37501 tgaactttct acttagaagg tcttttcact aattctgtgt atttaaatac ttaaaaatag
    37561 tatctaaaca cttttaaagg tggagtttta tcgtttcaga aattttagca taggcttgat
    37621 ctgttaggtg caaaccatct tttgtaaaac tgctagcaag ctgacctttt tcgtcaacta
    37681 ataaatcata caattcaata taattaatgc cagaaattac tgataaggtt ttgtttaaag
    37741 cttttattgc ttgattattt cttaccttga cacgctctat atagtcatct tcttcactaa
    37801 caggtaaaac actcagtata ttaatctctg tcatataaga ttccgcacga atttctgcaa
    37861 ttatatccgt gatattagct atgatttctg actgtgaatg ccctaaacca atatcatttg
    37921 ttccaattaa aataaatact ttggatggta atagttccca tacttgggtt ctcaagtttt
    37981 ctaaaagcca ataagaatct gatcctgcta ctcctctatt aactaaagga tagtcacgtc
    38041 ctaaatgttt ttttaaagga aaaaactcaa cgatagaatc tcctgtgaaa acaatggcac
    38101 cttcttgtac tgtctgattg ttaatctcaa aaaactgttc tcgtttcatt tgataatctc
    38161 ttaaagcttt gtcaataact tctaacatcg ttctccttca ttcttaaata tttctctagg
    38221 ctttcttgta ttataacaaa atttcacctg agaattgaat agtatgcttc gtgaaaagct
    38281 acgtggttct attcattatc actcatcttg tgaagaaagt tctctatcgt ttctaacaca
    38341 ccatcttgat cgttactaac tgtttgatat ttagcaatag ctttaatttc ttctggcgca
    38401 tttgacatag cgtaagaaat atttgctgct ctcaacattt ccacatcatt gcccccatca
    38461 ccaaaaacca tcacttgatc ctgtttcaat ccccactttt caaggagaat agcgatacca
    38521 tccgctttat tgacatcagc tggtaagaca tcgatacaac caaaaccact agaggtcaat
    38581 ctgatatgat gatttttaaa gtgttcacta atatctaaca acatctcaga aacaagattt
    38641 tcatttaccc aaagtgtcat ttgaaaatat cggtcatcat ctaagggcag caaatggtct
    38701 ttatagataa aattaggcat atagtattct aattcagtcc gcctattaat aggagttctt
    38761 cgctcaaggt atgaacgttt ttcaccagat aagctgatga gtgtctcagg atagttctga
    38821 tataaatagg ataaaacagc tgcaactgta tcattagata aataagtttc ttttagtaat
    38881 gtgccatctt ggtcaatgac acgaccacca ttttcagcga cgtaggcgat acgattaggg
    38941 tattccaaaa agtttaacaa caattgatcg tataaattac cactagcagc tacaaattta
    39001 atatcatact tctccatcaa cgttaataaa gaagaaaacc gtgctttatc ataagtttta
    39061 tctgagcgta gaaaagttcc atccatatct gttgctatta gtttaatcat acaagtaacc
    39121 tttgcctttc aagagggtgc caaatatctc aatatgttct ttcattat
//
LOCUS       DATRQA010000003        36352 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.003, whole genome shotgun sequence.
ACCESSION   DATRQA010000003 DATRQA010000000
VERSION     DATRQA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36352
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.003"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..243)
                     /locus_tag="VBD27_000105"
     CDS             complement(<1..243)
                     /locus_tag="VBD27_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7024151.1"
                     /translation="MAISIKSINLQNFRGFEDLHLNLNKYVTCISGHNGTGKSTILAA
                     LSNTGEYKKDKTLNNKPFRGEFEKIILGDPNYDKKIN"
     gene            complement(368..754)
                     /locus_tag="VBD27_000106"
     CDS             complement(368..754)
                     /locus_tag="VBD27_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO7024152.1"
                     /translation="MTIEEILDFHNVELAYFDNDLWPRPGIYVDEIKVVFVNKALSDE
                     SKKKVIFHELGHIDHDSNQYGRRHEEFELEANRFMIRCLLEDEFDEVEDKHEFNYLSF
                     MKRHNLKTTTDEVMVIDEYYNLLDAV"
     gene            complement(758..1105)
                     /locus_tag="VBD27_000107"
     CDS             complement(758..1105)
                     /locus_tag="VBD27_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7024153.1"
                     /translation="MFTTFDRVKELSQKRGISLSKLEETLGLGKNSIYALKRNQPSAE
                     RLQEIADYFNVSTDYLLGRTDNPNIAKDNQEYTSDDLRKMAEKAKTFDGKPLTDSDID
                     AIQNIIEIYLKGR"
     gene            1395..1586
                     /locus_tag="VBD27_000108"
     CDS             1395..1586
                     /locus_tag="VBD27_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO7024154.1"
                     /translation="MPDIANGRKKICDFLKENNIKKAALAAAYGMSRQEVTNILSGST
                     RGLKANQFILRVIEDYKID"
     gene            1620..2390
                     /locus_tag="VBD27_000109"
     CDS             1620..2390
                     /locus_tag="VBD27_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEO7024155.1"
                     /translation="MNEIFNFHGQEVRTVTINNEAYFVGKDVADILGYSNGSRDVNRH
                     VEEEDRLKYRFGTSGQDREMTIINESGLYSLILSSKLPQAKDFKRWVTSEVLPTIRKH
                     GLYAIDDLLENPDMAIAALQKLKEERQLRLQAQEEVAQKNQIIQELQPKATYYDLILQ
                     SESLVAISVIAKDYGMSAKKLNSLLHELKVQYKQGNTWLLYQKYASKGYTQSKTHPID
                     AERSKMHTYWTQKGRLFIYDLLKNKKGILPLIEQEEVA"
     gene            2468..2779
                     /locus_tag="VBD27_000110"
     CDS             2468..2779
                     /locus_tag="VBD27_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO7024156.1"
                     /translation="MPKAEIIYRPANQSEKATHGDYAHLCQVWEGLTIGTAKVWAAEM
                     REHPDFRQFIDNPTHRIVFIDYEGFRLFVKWKSRNRYRPKKETLSEMIDNIKREKQLG
                     V"
     gene            2784..2930
                     /locus_tag="VBD27_000111"
     CDS             2784..2930
                     /locus_tag="VBD27_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024157.1"
                     /translation="MKKLLKKLFVKKNRKIDVRPAWKIETHGWEANARRYDNMIEHNR
                     GRTC"
     gene            3059..3470
                     /locus_tag="VBD27_000112"
                     /pseudo
     CDS             3059..3470
                     /locus_tag="VBD27_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3619..3822
                     /locus_tag="VBD27_000113"
     CDS             3619..3822
                     /locus_tag="VBD27_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024158.1"
                     /translation="MNYLEYALAYLERELEIIDNEAIEVELPDGDWEFVLNPYYEEGL
                     HDNPYYRSQVAKDILDIKGLLGR"
     gene            3912..4211
                     /locus_tag="VBD27_000114"
     CDS             3912..4211
                     /locus_tag="VBD27_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024159.1"
                     /translation="MIEVTFKAESMAGVFDAMREALDMPKTVTKDVVEEKPAPKKKEE
                     SVTLTLADIKKMAKAKVEEGKSKDIKEVLREMNVAKVGELEEYQFAEFVEKLEAL"
     gene            4211..5368
                     /locus_tag="VBD27_000115"
     CDS             4211..5368
                     /locus_tag="VBD27_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEO7024160.1"
                     /translation="MPVENHALLSASSAHRWLYCPMLPRLEADYPSRDTVYTQEGTSA
                     HELSEIKLMYKSGKITKRKFNTLTKAFKENSDFYNEEMEEMTELYTDIVMEHLNAYEN
                     AEIELEKRVDFSDWVPGGFGTSDVVILADGVIEIIDLKYGKGMPVSANQNPQMGLYAL
                     GAYASYDMVYDFDRIKMTIIQPRLDSVSSVDIYVEELLYWADNVVLPMAAQADAGIGD
                     WNLSEKVLQWSPVAAKLVPRAQENWELIDKYDYQEPVYLSDEAVAEILDKASAIKKWV
                     ESVEAYALKEALSGKEVPGYKIVEGRSNRVITDKDKAIDILQDNGFDDEIFKPKELLS
                     MGALEKLIGKTTFADLLAEVIDKPQGKPVLVPEKDKRPAINSLEQAIKDFE"
     gene            5382..5945
                     /locus_tag="VBD27_000116"
     CDS             5382..5945
                     /locus_tag="VBD27_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEO7024161.1"
                     /translation="MTTTPNTTKVVTGKVRLSYVALLEPKAFEGQEAKYSTVILIPKT
                     DKATIKKIKDAQKAAYEAAKDNKLKGVKWERVKTTLRDGDEEMDTEEHPEYAGHMFMS
                     ISSKTKPQIIDKYKNSVDSAEEVYSGVYARVSLNAYAYNTAGNKGISCGLNNVQIVAK
                     GDYLGGRSSADADFDEWNEEEDEDDIL"
     gene            5988..7910
                     /locus_tag="VBD27_000117"
     CDS             5988..7910
                     /locus_tag="VBD27_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEO7024162.1"
                     /translation="MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVE
                     CLDLTRQSLPEDIKDMLFDDKVRKHAFNAQFERVCLSRYLGLPYYLDPCQWQCTMVLA
                     QELGLPSSLEKCALYLKLAQEKDTSGKNLIRYFSLPCKPSKTNGGRTRNLPEHAPEKW
                     QMFIDYCIQDVVVEMAIAEKLESVPVHDREWDYYVCDQRINDRGVALDKELVASALYC
                     KDVKMESLSGELKALTGLDNPNSRAQLLPWLKEHGYSANGLTKADVERELKTAEGELK
                     RVLELKLQTAMSSLKKYEAMERAMCSDGRVHGLLQFYGASRTGRWAGRVVQVQNLARN
                     YIKDLDDAREYVKKRDIDAVEILYDSLNDTLKQLVRTALVAKDCCTFYVSDFSAIEAR
                     VIAWFAGEQWRLDVFSTHGKIYEASASQMFGIPIEEIDKELRQKGKISELALGYQGGP
                     GALKQMGALNMGVKEEELQGLVDDWRRTNKKIVQFWKDVQRAAIKAIKSRAPIKLGKL
                     RFRYRKGFLFITLPSGRNLAYARAKVEPGDFGDKIIYEGQGDKAYFTAQETYGGKLVE
                     NIVQATARDILAEALLRIEAAGHGVVFHVHDEAIIEGSGLTIEEVNELMAQAPDWAEG
                     LPLNSEGYITKYYMKD"
     gene            7915..10299
                     /locus_tag="VBD27_000118"
     CDS             7915..10299
                     /locus_tag="VBD27_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO7024163.1"
                     /translation="MKQEKLIVKSSPLQELHIATGNSRTAKTWKNITLTWQELVERLE
                     KPTVTQETFAEYQKMSRAEKGQAKDVGGFVGGWLKQGKRKNENVQSRSLVALDADSPS
                     KDFLDRLDLLADYAYVLYSTHSHSKKAAKYRLIIPTDRLMMPDEYEPVARYLANQLGM
                     SNFDDTTYQSVRLMFWPSHSRDADFTFKYNDEAFLSVDEVLDTYPDWHDSSFWPESPT
                     HAVKRQREAKKQGDPLSKKGLIGAFCRNYDIRQAIATFLPEVYEEGTTPDRYTYTEGS
                     TANGLVIYDDVFAYSHHGTDPVGDTLVNAYDLVRIHKFGEQDSGAKDNTPTNKLPSSK
                     AMNAFVCDLPEIKDYLMAEALGDFDEELPVEDDRSWLEIDERGEPEVNSYLLATQIIK
                     EVPIYWDGFEFLRYDAKKGIWLPNAEEYLKSYISTKKLGKITKIRHISETIVAIKAQA
                     FSSEVFTESDLNKIVLANGVYDLRDNSFKTKFDPELHARSSHPVVYDPEATCETFEGF
                     LRETVGAENIDFIFEWFGYNFYREYTIQKMLFIYGSGGTGKSTLINILREMIGADNYS
                     AVTLQYLMQERFAKIGLYRKTANFDTDAKPQYLADGATLKMLTGEDTIHADRKNKEPI
                     NFYNYAKLSFAMNELPPMRDFSGGLKRRMMILEMDKVLTQEVKAKYPLDKIMGEVSGI
                     FNRAMEGLRKALSKRDFSISASMRSSVEKWEKGNDVVAMFLEDECEPGEDFKAPVRDV
                     YPAYKFYCQDSGYKPLAKNAFNHRLRELNYENKNVKSGGKQAKNWVGFRLKSEF"
     gene            10686..10961
                     /locus_tag="VBD27_000119"
     CDS             10686..10961
                     /locus_tag="VBD27_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEO7024164.1"
                     /translation="MRTEKDIENYLKKKTKGLCLKFTSPGTVGVPDRIVVMKTGVFFV
                     EVKAPGKKPRPSQILMHKKIKEVGQPVWVVDSYESVDIALKEMENWV"
     gene            10958..12280
                     /locus_tag="VBD27_000120"
     CDS             10958..12280
                     /locus_tag="VBD27_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO7024165.1"
                     /translation="MRLHEYQEYAKTWIVEHPYCGLLLDMGLGKTLTTLSAIDEIQNI
                     FSEDHKILIVAPKKVAEETWPTEIGKWHFDFTYSKVLGSEGKRIEALETEADIYLINR
                     ENVTWLVEYYKTKWPFTFVVIDELSSFKSSKSKRFRALRKVRPKVQRLVGLTGTPAPN
                     SLIDLWPQIYLMDRGDRLETSQTRFKDKYFVPDKRNGPIIYSWALRDGAEAEIYNKIE
                     DICVSMKAKDYLKLPPRTDNVVSVKLSNMKAYKQFEADLVLEFKNKEISAANSAVLAN
                     KLLQMANGAIYDDDKATVAIHDDKLDALENIVEESQGQPILVFYQYQHDLERLKKRFP
                     QAEELTSVDKWNSEKIPILLCHPQSAGHGLNLQKGGHIIVWFGLTWSLEYYQQANARL
                     DRQGQTEPVIVHHIVAENTVDEKVLRILQGKEKNQNALLEAVKAQLGV"
     gene            12281..12451
                     /locus_tag="VBD27_000121"
     CDS             12281..12451
                     /locus_tag="VBD27_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024166.1"
                     /translation="MKKEYVVRIYTGREKNLEAKPQFEEKTFTRKADMLKFWNSCEAT
                     VKEKYTREREND"
     gene            12733..12927
                     /locus_tag="VBD27_000122"
     CDS             12733..12927
                     /locus_tag="VBD27_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024167.1"
                     /translation="MKIEFFLKQGPTKSVSNYGEVGIDEVIGAIKNNDIVALNGKNNA
                     VIIPKQNISHVIIHKEVTNE"
     gene            12920..13336
                     /locus_tag="VBD27_000123"
     CDS             12920..13336
                     /locus_tag="VBD27_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HEO7024168.1"
                     /translation="MNKLSNAQLKAFDEWLFDYRFIDNKIALRKLELQTDMNTEVDKN
                     IGGGRANFVSKVTEDVVARWDSDRELKGLSNFKEAVIATLNLLDEELTDIFNLRWGVG
                     SSNTWEEIAYTRHLSRKSIYRKRERILEIFSKKIGM"
     gene            13426..13878
                     /locus_tag="VBD27_000124"
     CDS             13426..13878
                     /locus_tag="VBD27_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEO7024169.1"
                     /translation="MLGGDGKLSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARS
                     IGQENLTKPDIKAYIDERLEKLESEKIATQEEVLQYLTSIMRGDQQEKTLISVGEFGQ
                     KIVDIDVGAKDRIKAAELLGKRYRLFTDKVEMDVSSDITISVGEWDDD"
     gene            13868..15145
                     /locus_tag="VBD27_000125"
     CDS             13868..15145
                     /locus_tag="VBD27_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEO7024170.1"
                     /translation="MTIKQRPKINIVIKHPSKVFNKHIYDKLYNYSNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGVLDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQVYLMFNPVSKANWVYKAFFIKSPKNTVVYQTTYKDNRFLDDVTRENIEELAD
                     RNEAYYKIYALGQFATLDKLIFPKYDKQILNKDKLSHLPSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYILEEYVRKNLTNDKIANAIKDLGYAKEEIRGDSAEKKSNQELRNLGIP
                     RMIDVTKGPGTVMQGIQYLLQYDWIVDERCVKTIEELENYTWKKDKKTNEYINEPVDS
                     YNHCIDAIRYAVQDRIYQSADRSKRMKNAKYYF"
     gene            15161..16693
                     /locus_tag="VBD27_000126"
     CDS             15161..16693
                     /locus_tag="VBD27_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO7024171.1"
                     /translation="MEEKQFLVGTRFNENANKQFIMLQEDFEAIDYESKLWIDQLKNY
                     ISRFKAEQLERLKELKRYYLGDNNIKYRPAKTDKYAADNRIASDFAKYITVFEQGYML
                     GVPVEYKNENKDLQAAIDLMSVRNNEDYHNVKIKTDLSIYGRAYELLTVEEIDDKKTE
                     VKLYQLPAEQTFVIYDDTYQRNSLMAVHFYDIDYGSGKRKQIIKVYTSDTIYTYEDYN
                     FETKGMRLKEHEEHFFKGVPVNEYANNEERTGAYESVLDNIDAYDLSQSELANFQQDS
                     VNALLVIAGNTYTGADENDYLDDGRLNPNGRLAISIGFKKAQVLILDDNPNPNGVKPQ
                     AYFLKKEYDTAGSEAYKNRLVADILRFTFTPDTQDMKFSGVQSGESMKYKLMASDNYR
                     EKQERLFKKGLMRRLRLAANIWAIKGNEATTYSLVNDTSIVFTPNLPQNDNEIVTAAQ
                     NLYGIVSDQTIFEILNTVTGVDAEAELERLKEEADKKQSLPEPRLVGDASGQEEPTAE
                     KP"
     gene            16653..18101
                     /locus_tag="VBD27_000127"
     CDS             16653..18101
                     /locus_tag="VBD27_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEO7024172.1"
                     /translation="MLVVKKNQQQKSLNYWQKRQEDILSYLDRTDLDVFSELQKLYNE
                     QAFELQKELFDFYTKYSEENKMTYQDVVKKLRHEDLSDYVANANKYRKQAEKDPELLK
                     RLNEQYVSARATRMDALNLELVYRAGILKGVLDSVFENHLKKAASYAYKKAMGGRSGT
                     INGPVLEELVRTPFDGYNYSEQLWGNTDNLVKNLQKKLKQGFVRGEHPRAIARDLAKQ
                     FNVANHRAETLIRTDGTMVINNATARRYLNAGLKYYRDLVRLDDRTTEICRKIAKENK
                     RKLLSELKPGINAAPYHFNCRTTIIPDEDELSIEVDPIDDKSTKYFKDVTSDWIDGNE
                     HKPQLSLLNEYVKNGTPYKVDGHNVVLDHSNYEYRVANWLSKKTGLQVDMVPRVNSPE
                     HINTPDYLVDGVPFDLKEITGSGKNVIDGNLRKAKKQATNIIFDITKTPLSFEEIMGQ
                     LEHIYMLDRRGLDISIIKNKDEVLAVLEKEGR"
     gene            18129..18317
                     /locus_tag="VBD27_000128"
     CDS             18129..18317
                     /locus_tag="VBD27_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024173.1"
                     /translation="MGVRPSSFFISIIHQPIKTIKEENMFIWQMVLVALGVLVLILIV
                     GIAAIAVKSIIAELKKVG"
     gene            18320..18586
                     /locus_tag="VBD27_000129"
     CDS             18320..18586
                     /locus_tag="VBD27_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024174.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAMQAEALRNQNKQITELRAIVQ
                     QNAQATNEEFASVKAATLDNQSAIADIAIDVNYIKKNYKRKWGK"
     gene            18742..19317
                     /locus_tag="VBD27_000130"
     CDS             18742..19317
                     /locus_tag="VBD27_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEO7024175.1"
                     /translation="MAEENVTTETTEQVDTQQETVEHPKQEHERTFTRADISKMMTAE
                     RAKWEAEQSEAIEKARTEGERLAKLSKDERAKEEEQKRLSAIAEREKAVAEREMRIET
                     QSLLAEKGLPLDFIDVVLATTADEVKTNIDNLQSIFDQAVENRVNERLTQKSPRTGNG
                     SVGMTKAEIMAIENDDERLKAIAANRNLFTR"
     gene            19336..20220
                     /locus_tag="VBD27_000131"
     CDS             19336..20220
                     /locus_tag="VBD27_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEO7024176.1"
                     /translation="MAENNLNVMSDLGEIKSIDFVNKFSTNINELLKLLGVTRKQELT
                     NDLKIQTYKWSTTLDKTNPAEGETIPLSKVTRAKAKEYTVEWFKKRRATTAEAIARHG
                     VARAINEADTRIMREIQNGFKTNFFEFLKKSTTKVTGTTLQSALSQSWAKIATFNEFD
                     GSPIISFVSPLDVAKYLGDTNVGADASNVFGMTLLKNFLGMQNVIVLPSVPEGKVYTT
                     AVENLVFAYLNVNSGDLGGMFADFTDETGLIGAARTRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEAPKVVPGG"
     gene            20224..20580
                     /locus_tag="VBD27_000132"
     CDS             20224..20580
                     /locus_tag="VBD27_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO7024177.1"
                     /translation="MDKELLLTEVKLFKGISAKDTSQDDLIKLSINESIDRILAKLND
                     YSDTEMTEIPKRLTFIVRDVAIKRYNRLNSEGTVEDSEEGKTFKWDSYLKEYESTLRS
                     AAIGKVYSGKGVARFI"
     gene            20591..20869
                     /locus_tag="VBD27_000133"
     CDS             20591..20869
                     /locus_tag="VBD27_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024178.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGRYDGFSKIIYKGKPRLIKGLAHHKNSTVIYV"
     gene            20866..21210
                     /locus_tag="VBD27_000134"
     CDS             20866..21210
                     /locus_tag="VBD27_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO7024179.1"
                     /translation="MSLIYRMRGLDRFLRSVERKQKSVRIAVDKELSKSAARIERQAK
                     ILAPVDTGWLRAQIYSEQQRLLHYRVVSPALYSIYLELGTRKMEAQPFLDPALRKEWP
                     VLMANLKKMFKR"
     gene            21214..21573
                     /locus_tag="VBD27_000135"
     CDS             21214..21573
                     /locus_tag="VBD27_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024180.1"
                     /translation="MDYSPETLYLKKVKNRLGVLDIPIYFKLPKSDVLEPFIVVGTNI
                     SDLSKTAQTGAVIDDFSLNIDAFLPGDSRLDAEEIKSRMLRLLGRNNQIKAQILVDNS
                     IGREVYRVAINITEILI"
     gene            21585..22217
                     /locus_tag="VBD27_000136"
     CDS             21585..22217
                     /locus_tag="VBD27_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEO7024181.1"
                     /translation="METTVEITTAKPIKGKNIFYFIQSVDAELGSKAILPAYRTDGTT
                     KLGGDFIDEQTQQGRVLEKATDEHSIDLTSYYTPKDKAIEIVEIAKTTGKSVKVWRVI
                     ADESLKEKGSGTLEGKEVYPAKFGYGKIDEIEYSEGVEKLVEASYTLNIIDSLKDGKF
                     PLTAEDIALLTSVYAYQNPGETTGDYDNIQKKEVSGSLLGAVPGISSEGH"
     gene            22268..22723
                     /locus_tag="VBD27_000137"
     CDS             22268..22723
                     /locus_tag="VBD27_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024182.1"
                     /translation="MELTVAKRSIEIKFDFKTMFKINNRLGTVNKETGERNADGVGTL
                     FYKILERDDSAVVDLVKLSVGSGKKALSEDEVLDSIAELVEEEGSTEALFKEIENDMV
                     ESGFFKEKISKYIESMEKSVKYLEAREDADEAQIQIVKDMIGKMKDAIS"
     gene            22798..23028
                     /locus_tag="VBD27_000138"
     CDS             22798..23028
                     /locus_tag="VBD27_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024183.1"
                     /translation="MEGLQYKQIAERENLSELALNLRYTLNAKNVNANKLKLDKQRNK
                     IKRTYQNKQDLTNVSNDFAERVAKLNLHFQNL"
     gene            23047..27273
                     /locus_tag="VBD27_000139"
     CDS             23047..27273
                     /locus_tag="VBD27_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEO7024184.1"
                     /translation="MAGTFDGSIFADIGANTKDYEAAMARIVSTTQNAFRKAQDTAVN
                     NSNKMIQMIGQIMAQLANNGQSLGQRLGNAYSTGLKLSLGQIQRIAASIGEKIPEPIK
                     NGFNKAFSIIQNSVQKLSSAIPQPIKSAFTSATSAVVSFSSKITSAVSAAFNSVSSKA
                     STVADKISSSFGGRITSAVTNLATKLSTGLSNGFSRMSSSATSALNGISQRFASASSA
                     GEKLRSTVGSIISAFSLMAIARKGIQAITGAMDGAISRVDTMNRFPKTMALFGYSAQQ
                     SKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTELALAFNNAMIGYGATTE
                     GAEQALRQFNQSLGSGKIYAEEFNSVSEAAPGLMSKMAESFGFGKNGVQDLKAALSDG
                     SITAQEFADKMIELNKAQGGFAEMAQSSAGGIRTAWKNVHTAVVKGVAGMISAFDEAA
                     KANGMKTIAESINSIKPAINSAFATVNSIMPNLVAAFARISKMVNIDFSPLTSGIKSA
                     FGLLNTALGEFAKTGEVSSATIDRIKSKIASIGPTAIAAWALLNPASAIATIMPLISI
                     IGKIGVALGTVGSALGSFGEMVASGLSTASGLVGAFGRAMSGLPGIFGVAASRGLAVL
                     DGMTTAISSAAILAMSAIGPAAILGLVVVGLGLVNNQFGTQIDKLINTAITKGPGIIQ
                     GLVKGITSKIPALIASGTQLIAKFANALTVLLPVIIQAGVQLITSLVQGIGQNAGSLI
                     SSALKVIGTFVSSIASALPQILAVGVQLILNLARGIVQNIPLIIQYAQQIISNFGSSL
                     QANMPSIISNGIAISTNLNAGIIQMLPAVIQIATQVITGFISGIAQYLPQVIQGGIQI
                     IIMLVQGIIQNLPQVIQAAMQIIQSLISGLSQALPQIISAGIQLVVQLAVAIIKGLPQ
                     IISAGIQLIAGLGKAMLEAIPNALSGVWEGIKGGFSSMWDQITGKSSTSAAKVTSDMS
                     TMTMNVGMQTTTMANQTNIDTTTMLNSISQNTGLANLNATTNATQMATNVNAQTASMA
                     NQGLLNAAGFNEALSSNMTLANTNATNQANQMTANVNNATSQMSLGSLPNFLELSQQA
                     TSNMQTAQANATTSANGMSSGVTGATAGMASGVANNMLGITSTVSTGMNQAANAAKSG
                     GANISNSVNSSFTQAKSTVSSSMSGMNSTVTSGMNSINNTVKSGGNNIKSTFTSSFNS
                     AKSTVSNAMSSIRSSISSGMNSAVSVTRSAMNSVVSAMNAAASGARSAGYNTGIGFAN
                     GLASTAGTIMAIANSIAANVAATIRSAMDIHSPSRVTRELGAYTGEGFALGMQDWMDD
                     INAIGKEYANAVTDQRWETDSQLATSANISSAGVSSSLDNLSDEVRNQQLTSPTFEVH
                     NELVGDEIYTTIKSKEARESNKDNYFNFAT"
     gene            27286..28128
                     /locus_tag="VBD27_000140"
     CDS             27286..28128
                     /locus_tag="VBD27_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO7024185.1"
                     /translation="MDLLVQKSDQSFRFSEIGLRILDVDDRSSSLEVDSRTVKGRSGK
                     IFASAKYGTKKVKVNGRLVVASIKDFMTKKDDVNGLLVDSEPFYITKMYPQKENLYDF
                     ELPGMKAGDLDLLNQSYTAWHYRWKVYVSSEPEYTFVGKSSEGLKYNFTIAFETAELP
                     FGETIAQNIVITNGTFNYSGTAPFSQLEYPWSVELTSTGGQSSFYLEIDGRRWAYQYS
                     APLNQGDVIVITGISTTLNKINVTARTNHEYFVLKQKVNKTILYSTNFQGTIKFLNFK
                     ELYK"
     gene            28141..31968
                     /locus_tag="VBD27_000141"
     CDS             28141..31968
                     /locus_tag="VBD27_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7024186.1"
                     /translation="MTTFLDHKDNEFEAQAVVKTTNAVNGERSLSGTIYTNEEVLNKV
                     DKGWKLEFDDEYYKIIYAKPTDTGNKIQVEFDAVHQFFYDFDKSSLHKQLNDGSHTFQ
                     AYLNFIFDGSGYTYSLEVVVKSFEKQSFGYKSRLDLFNDIIKSAGVEFFVRGKVVRIL
                     QKTGTDLSTIVRKNFNMNEIIIEKKINDFITYQKGFGAWADQEDHSKGRLEVEYESPL
                     AKEYGRIEGEPVVDERYTNADNLKSVLKQNVDSSYNIAVKIDIEDLTKAGYQYTQPIA
                     GDYIMAINETLGFKEKIRIVSFESEYDVTGQLINHKVTCNDIGTIKKQTVAVSQLSSK
                     INNNQAILDNAVFKANQALASADGKNTNYYGTEMPVDNPKGTLRKGDLLFLTVGDTTR
                     MYFWNGAEWIINSFSDDIDFVKKEITNKIIEVNAAMKLADEQLTEKYNAIVAKNVSQD
                     ELINQAKTLSDSAKKEATEALTNLISESKKLTDQMSALSKTEVEHYSTTNQQLTQIDG
                     TVKGLQTSYEALLKKDGDITQALANYKQTIDQNSASIMTNKKTADNALSSLQTQVIQT
                     SNEITTRLSQTNIEALITSKSAVIADNKVKETADSFSRELTRVNNTIDNLKIGTVNLL
                     SGTKGWTKNWFNRNNWQADDDSLSIGNFNLTVLKRKMMWSGISQSYAVKSGESYVFSA
                     YAKSSIDNDVVNLYLLPNDQSQQAKISISFKSITLNTDWQRIVIKFNVNSDGYILPRF
                     ERFNENGWLYIAGYQLEHGNVTSDYSESPADQEDKMTVEFNKINDTVNSHSQLIGKQN
                     ESLTATIQKVDNIQSTVSNVDGRLSRVTQTADGIVATVSQLDNNILPGTKKFTGWQTE
                     GAVLEELTQSPYPFVFKKWVSGNKVSPLIEYDVKKDQEYTFTAYISREQAGNLYFYLY
                     DLWNHHITSTTPRETIIKDVTQTIRRFKITFIPTRDGKIRPRFAMLASDPGSFSTGGF
                     MLSKGTVELEWSESKQDIKDSVTAINTTVTQTANGWAIKNLTSSGTVLSQLNLTDGNV
                     KLEGKLIHLSGQSIIDNGIITNAMIKDLTADKITAGTIDANRINVINLDASNITSGRM
                     SADLIRSGVLISQNGAMLADLNTGQIEFYTDSPAIKRITAGYPNQFVKFATGNVEGKG
                     AAGVTVIGSNRWGSESSNDGGFVGIRAWNGKDIDTLDLVGDSIHLASSAYTNADGWEI
                     ITLPNQLSIDARNINHRVTSKIKVGDIWLWKNTSTYTSMKDTINMIIDNLQLLHNNKT
                     TEKGYSYTIPGKI"
     gene            31977..32129
                     /locus_tag="VBD27_000142"
     CDS             31977..32129
                     /locus_tag="VBD27_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024187.1"
                     /translation="MEEKIKSNLALQIAQLSLDKATLQAQNEQLQLQIAELNQQLDEI
                     TKPGAK"
     gene            32140..32556
                     /locus_tag="VBD27_000143"
     CDS             32140..32556
                     /locus_tag="VBD27_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO7024188.1"
                     /translation="MKQWNIVGKPFPIYQDGVVLKTDVRLQGDDGTYLPQELIGNHVD
                     KDNQSLIKLVLETFAKENVVDFAMLESVKDIEQLKADKVTLIKKIAEADEAISKMSQA
                     TKDMQLKVDKAVVELTDLVTSSVSSLSVPSTSMESS"
     gene            32619..32780
                     /locus_tag="VBD27_000144"
     CDS             32619..32780
                     /locus_tag="VBD27_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024189.1"
                     /translation="MTYTFKKDHLIVDVWYRRVANGICDFEAVPKLFNLRDVVLELLS
                     QKVTDKESE"
     gene            32790..33080
                     /locus_tag="VBD27_000145"
     CDS             32790..33080
                     /locus_tag="VBD27_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024190.1"
                     /translation="MIDFVQVGAFAGAALSILGVWGFIVNPFKKAMEANEIAMAQLKD
                     SLKELAYELKNIDRDREITKAVIDRHEERLGRVEDDIIINKEQIKTLFNKEK"
     gene            33082..33333
                     /locus_tag="VBD27_000146"
     CDS             33082..33333
                     /locus_tag="VBD27_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO7024191.1"
                     /translation="MINWKVRIKNKAFWSAIIPAIFLVVQAVANVFGYTLELSDLGNK
                     LLVVVNSGFSVLVIAGIVTDPTTQGVSDSSRALSYTEPK"
     gene            33463..34806
                     /locus_tag="VBD27_000147"
     CDS             33463..34806
                     /locus_tag="VBD27_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEO7024192.1"
                     /translation="MATYQEYKSRSNGNAYDIDGSFGAQCWDGYADYCGFLGVPYANC
                     TNTGYARDIWEQRHENGILNYFGEVETMQAGDVAIFMVVDGVTPYSHVAIFDSDAGGG
                     YGWFLGQNQGGPNGAYNLVKIPYSATYPTAFRPKVFKNAVTVTGNTGLNKGDYFIDVS
                     AYQQADLTTTCQQAGTTKTIIKVSESIAWLSDRHQQQANTSDPIGYYHFGRFGGDSAL
                     AQREADLFLSNLPSKKVSYLVIDYEDSASADKQANTNAVIAFMDKIASAGYKPVYYSY
                     KPFTLNNVDYQQIIAKYPNSIWIAGYPDYEVRSEPLWDYFPSMDGVRWWQFTSVGVAG
                     GLDKNIVLLADDSSKVDIPKIDKPQEPQSQLTFNQKLDTNTKLDNSNVPYYEATLSTD
                     YYVESKPNASSTDKEFIKAGTRVRVYEKINGWSRINAPQSEQWVEDAYLIDATDM"
     gene            35757..36269
                     /locus_tag="VBD27_000148"
     CDS             35757..36269
                     /locus_tag="VBD27_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024193.1"
                     /translation="MIVDLKEIVNDYELNFCGKRCKVETNFKHLPEFMILFDIRDLYH
                     LLGIHKLKTKYRATNWVEAVKADVFLLSNYSKHPNFREVLPRVDNYNFLYEIFYQFRV
                     NVCILDKDLTKNTMKLSVVFYKDNKKKLVVVGLKRDETGVFRPATLHESRNNPYKRIR
                     HTAIKSITWI"
BASE COUNT        12491 a         6015 c         7672 g        10174 t
ORIGIN      
        1 atttattttc ttatcataat ttggatcacc aagaatgatt ttttcaaatt cacccctaaa
       61 aggtttatta tttaaagttt tatctttttt gtattctcca gtattactaa gagctgcaag
      121 tatagtagat tttccagttc cgttatgacc agaaatacaa gttacatatt tatttaaatt
      181 aagatgtaag tcttcaaatc ccctgaaatt ttgaagattt atacttttta ttgaaatcgc
      241 catataactt ttccttttta taacctaatt agcttacttt atttagaatt tttatatttc
      301 taatttaata atgtaattga attattaaga ttcaaaactt gttccacaat cagcgcaata
      361 ccattgatta tactgcatcc agcagattgt aatactcgtc gataaccatg acttcatcag
      421 tcgtggtttt taagttgtgc cttttcataa aagataagta attaaactca tgtttatctt
      481 ctacttcgtc aaattcatcc tcaagtaagc acctgatcat aaatctatta gcttcaagtt
      541 caaattcctc gtgtctgcgc ccatattggt tagagtcgtg gtcaatatgt ccgagttcat
      601 ggaagataac tttctttttt gattcatctg atagtgcttt atttacaaag actactttta
      661 tttcgtcaac atatatccct ggacgtggcc acaaatcatt gtcgaaataa gctaattcta
      721 cattatggaa gtctaaaatc tcttcaatag tcatagacta tcttcctttc aaataaattt
      781 caataatatt ttgtattgca tcaatatctg aatcagtaag tggcttgcca tcaaatgttt
      841 tagccttttc tgccattttt ctaagatcgt cagaagtgta ttcttggtta tctttagcta
      901 tattggggtt atctgttcta cctaataagt aatctgtaga tacgttgaag tagtctgcta
      961 tttcttgtaa cctttcagca gatggttggt ttcttttaag agcatagata gaatttttgc
     1021 ccaatcctag agtttcctct aatttactta gggaaattcc tcgcttttgg gataattctt
     1081 taactctatc aaaagtcgta aacattgatt ttatcaacct ttctagggta tgacaaaaaa
     1141 tatttagaga attttgtaga aaactgttga caaacagtag agaatactct aaaatagttt
     1201 ttgtaaataa gatttaacta gaaaaacacc taaacaaata aatcaaaaaa aagtgtttta
     1261 gcgaacggta ttcattgatt tattagcttc tttttctatg ctttcatttt agaatattct
     1321 ctaaaatatg tcaataaaaa acgcaaaaaa atagttgttt cttatttaca attttaaata
     1381 aaggaggaag aaagatgcct gatattgcaa atggacgcaa aaaaatttgt gacttcttaa
     1441 aagaaaacaa tatcaaaaaa gcagctttag cagctgcata tggcatgagt cgacaagaag
     1501 ttactaatat tttaagtggc tcaactcgtg gcctgaaagc taatcaattt atcttacgag
     1561 ttatcgaaga ttataaaatt gattaaatcg caggaagagt ttgaagaaag ggataatgta
     1621 tgaacgaaat ttttaatttt cacggacaag aagtccgcac agtaacaatc aataatgaag
     1681 catactttgt tggtaaagat gtggcagaca ttcttggtta ctcaaacggg agccgagatg
     1741 taaatagaca cgttgaagaa gaagataggc taaagtaccg ttttggtacc tcaggtcaag
     1801 atagagaaat gactatcatc aatgaatcag gtctttactc acttatctta tctagcaagt
     1861 taccgcaagc aaaagatttt aaacgttggg ttacgtcaga agtcttaccg acaatccgta
     1921 aacatggatt gtacgctatt gatgatctgc tggaaaatcc agacatggca atcgcagcac
     1981 ttcagaagct caaggaagaa cgtcagctac gtttacaggc acaagaagaa gtagctcaaa
     2041 agaatcaaat tattcaggaa ttacaaccaa aagcaactta ttatgatttg attttacaaa
     2101 gtgaatcctt agtggctatc tctgtcattg caaaagatta cggcatgagt gctaagaaac
     2161 taaatagtct gttgcatgaa ttgaaagttc aatataagca aggcaatact tggctactat
     2221 atcagaagta tgctagcaag ggttacacac aatccaagac gcatccgatt gatgcagaac
     2281 gcagtaagat gcacacttac tggacacaaa aaggacgcct gttcatctat gacttgctaa
     2341 aaaacaaaaa ggggattttg ccattgattg agcaggaaga agtggcgtaa taaaaaaagc
     2401 acctaacgaa gtcaggcgct caacaaaaat aactaactag attataacac agaaaagaga
     2461 gtttaacatg ccaaaagcag aaattattta cagaccagct aatcagtctg aaaaggcaac
     2521 gcatggagac tatgcccatc tttgccaggt ttgggaaggt ttgacaatcg gaacggccaa
     2581 ggtttgggcg gcagaaatgc gagaacatcc agactttagg cagtttattg acaatccaac
     2641 gcaccgtatt gtctttattg attacgaggg tttccgtcta ttcgtcaaat ggaagagtcg
     2701 caatcggtac agaccgaaaa aagaaacatt gtctgaaatg atagacaaca ttaaacgcga
     2761 aaaacaatta ggagtataac atcatgaaga aattattaaa aaaactattt gtaaaaaaaa
     2821 atcgaaaaat agatgtgcgt ccagcatgga agattgaaac acatggctgg gaagctaatg
     2881 cacgacgtta cgacaatatg attgaacata atagagggcg tacatgttaa aagaagtatt
     2941 actagaaaat gaactgttgc gtgatgaaaa tagacgacta actaatgaaa tggcagatta
     3001 ttattttacg aacgtagcaa aagcaaattt attggatatt atcgtcaatg aaggctacat
     3061 cttagatagc acactggaaa aatgtattgc taagctagat aacgttgata gacaaatact
     3121 agaaaaaaat tggagtgatg ataatgattg aagttaaaag acgagatagc ttacctatcc
     3181 tatctgctca gttgagtgtt atcaagcaag atttaaaatt gatgctttgg attgagctac
     3241 cgacgttaaa tgacgtagag aaatatgaag ctctttatca aggaaatgat tttaaattca
     3301 gtgaaacttt tttggagcgt caagcgatga gaaaagcctt ttggagacga ttagctaagg
     3361 aaacttttaa agaagaccag gataggagga actttgttaa gttggctgag aaagaattta
     3421 aaggtgcaac tttgtcatgg caagaatttt tgtggggtaa aaagaaatga atatttcaat
     3481 aaaactcata ggtgaaaccc tggctggttt aaatgatatt ttacgccggg ggggggggct
     3541 ctcttgctcc caaaaccaag ccttagcaga cgcagttttt atattaacaa ctttaaaaca
     3601 agtaattgag gagagaaaat gaactactta gaatacgcac tagcttatct tgaacgtgag
     3661 ttagaaatta ttgataacga agctatcgaa gttgagttgc cggacggaga ttgggagttt
     3721 gtgcttaatc cctattacga ggaaggttta catgataacc catactatcg tagtcaggtt
     3781 gctaaagaca ttcttgatat taaaggactt ttggggaggt gaggctattg attgcatcaa
     3841 aaaagacggc ttaggaaacc gtcaacaatt acacacacac ttaaattata acacagaaag
     3901 agagaaacac aatgattgaa gtaactttta aagcagagag catggcaggt gttttcgatg
     3961 ctatgcgtga agctctggat atgccgaaga cagtaaccaa ggatgttgtt gaagaaaaac
     4021 cagctcctaa aaagaaagaa gagtctgtaa ctttaacatt agctgatatt aagaaaatgg
     4081 ctaaagccaa ggttgaagaa gggaagtcaa aggacatcaa ggaagtcttg agagagatga
     4141 acgtcgcaaa agttggtgaa cttgaagaat accagtttgc ggagtttgtt gagaagttgg
     4201 aggctctcta atgccagtag aaaatcacgc actactgtct gcttctagtg ctcacagatg
     4261 gctttattgc cctatgctac cgagattaga agcagactat cctagccgcg acaccgtata
     4321 cactcaagag ggcacaagcg cccacgagct ttctgaaatc aaactgatgt acaagtctgg
     4381 taaaatcacc aagcgtaaat ttaacacgtt aacaaaggct tttaaggaaa actcagactt
     4441 ctacaacgaa gagatggaag agatgacgga gctttacaca gatattgtta tggagcacct
     4501 aaatgcttat gagaatgccg aaattgaact tgaaaaacgg gttgatttta gcgactgggt
     4561 gcctggcggt tttgggactt cggatgtcgt cattttggcg gatggggtca ttgaaattat
     4621 tgatcttaag tatggcaagg gtatgcctgt gtctgctaac caaaacccgc agatggggtt
     4681 gtacgctctg ggagcttatg cttcctacga tatggtttat gactttgacc gtatcaagat
     4741 gaccattatt cagccacgtt tagattcggt tagttctgtt gacatttacg tagaggagct
     4801 tctgtattgg gcggataatg ttgtcttgcc tatggcagct caagcagatg caggcattgg
     4861 tgactggaac ctaagtgaaa aagtattgca gtggtctcct gtcgcagcta aattggtgcc
     4921 aagagcgcaa gaaaactggg aattaattga taaatatgac tatcaggagc ctgtctattt
     4981 atctgatgaa gccgtcgcag agatccttga caaagcctca gctatcaaga agtgggttga
     5041 gtcagttgaa gcctatgccc tgaaagaagc actctcagga aaagaagttc caggctataa
     5101 gattgtcgaa ggtagaagta atcgagttat cactgataaa gacaaagcaa ttgatatttt
     5161 acaagataac ggctttgacg atgagatttt taaaccgaaa gagctgttat caatgggagc
     5221 tcttgaaaag ttaataggta aaaccacttt tgctgattta ttagcagaag taattgataa
     5281 accacagggc aaacctgtac ttgtccctga aaaggataag cgcccagcaa taaacagttt
     5341 agaacaagca attaaagatt ttgaatagga gaaaaataat tatgacaaca acaccaaaca
     5401 caactaaagt agtgaccgga aaagtacgcc taagctatgt agctttacta gagcctaaag
     5461 cctttgaagg ccaagaggct aagtattcaa cagttatctt aattccaaaa acagacaaag
     5521 ccacaatcaa gaaaattaaa gacgcgcaga aagctgctta tgaagctgcc aaggacaata
     5581 aactcaaagg agttaaatgg gagcgcgtta agacaacgct tcgtgatggt gacgaagaaa
     5641 tggataccga agagcaccca gagtacgcag gccatatgtt catgtcaatc tcaagcaaaa
     5701 ctaaaccgca gatcattgac aagtataaaa actctgttga ttccgcagaa gaagtctact
     5761 ctggcgtcta tgctcgtgta tcacttaatg cctatgctta caacacagcg ggaaataaag
     5821 gaatctcttg cggattaaac aacgtccaaa ttgttgctaa aggagactac cttggcggcc
     5881 gttcgtcagc tgatgcagat tttgacgagt ggaacgaaga agaggacgaa gacgacattt
     5941 tatagttgag ggcctcttta gaggctcctc atttttaaag gagaggtatg agacatttaa
     6001 atattgatat tgaaacctat agttcgaatg acatcaaaaa tggggtttac aagtatgctg
     6061 acgcagaaga ttttgagatt ttacttttcg cttactctat agatggcgga gaagtagagt
     6121 gccttgattt gacaaggcag tctctacctg aagacatcaa agatatgtta tttgatgata
     6181 aagtccgaaa gcacgccttt aatgcccaat tcgaaagagt ttgtctcagt cgttacctcg
     6241 gcctacctta ctatctagat ccttgccaat ggcaatgcac catggtgctg gcccaagagt
     6301 tgggtctgcc ttcaagcttg gaaaagtgtg cgctgtattt aaaattagca caggaaaaag
     6361 atacctcggg taaaaactta atcagatact tttccctgcc ttgcaaacca agtaaaacta
     6421 acggtgggag aactagaaat ttaccagaac acgcccccga aaagtggcaa atgtttattg
     6481 actactgcat ccaagacgtt gttgttgaga tggcaattgc cgaaaaacta gagtcagttc
     6541 ctgtgcacga ccgtgagtgg gattactacg tctgcgacca gagaatcaac gacagaggcg
     6601 tggcgcttga taaagagtta gttgcttcgg ctttgtattg taaagatgtt aagatggaaa
     6661 gtttgtctgg tgaactaaaa gctctaacag gacttgataa tcctaatagc agggcgcagc
     6721 tgctaccgtg gctaaaagaa cacggttatt cggctaatgg gctgactaaa gcagatgttg
     6781 aacgggaact taagacggcc gaaggagaac ttaagagagt cttagaactt aaactacaaa
     6841 ccgcgatgtc aagtctaaaa aaatatgaag ctatggaaag agctatgtgc tcagacggac
     6901 gagttcatgg actacttcag ttttacggag ctagcaggac aggaagatgg gcgggcagag
     6961 ttgtccaagt acagaactta gctaggaatt atataaagga tctagatgat gctagagagt
     7021 atgttaaaaa gcgtgatatt gatgctgtgg agattttata cgatagtctt aatgacactt
     7081 taaagcagct agtaagaacg gcactcgtgg ccaaagactg ctgtaccttc tacgtctctg
     7141 acttctcagc gattgaggct agggtgattg cgtggtttgc cggagagcag tggaggcttg
     7201 acgtgttttc gacgcacgga aaaatctatg aggcatccgc tagccagatg ttcggaattc
     7261 caattgagga gattgataag gaactacgcc aaaaaggcaa aatctcagag ttggcacttg
     7321 gctatcaagg aggtcctgga gcgctcaagc agatgggggc tctaaatatg ggggtcaagg
     7381 aagaggagct tcaagggtta gttgatgact ggcgcaggac caataagaaa atcgtccaat
     7441 tttggaaaga cgtacaaaga gccgccatca aagccatcaa atcgagagca ccgataaaac
     7501 ttggaaaact acgatttaga taccgtaaag gtttcctctt tataacattg cctagcggta
     7561 ggaacttagc ttatgcaaga gccaaggttg agcctggtga ctttggagac aaaatcatct
     7621 atgagggcca aggagataag gcatacttca cagcgcaaga gacttatggc ggtaagcttg
     7681 tcgaaaatat cgttcaggcg acggctaggg atattctagc tgaagcgctt ctgaggattg
     7741 aagctgcagg ccatggtgtt gttttccacg ttcatgatga ggctattatc gaaggctcag
     7801 gcctgacaat cgaagaggtt aatgagttga tggctcaagc gcctgactgg gcggagggtc
     7861 ttcctttaaa tagcgaaggc tacataacaa agtattatat gaaggattag atagatgaag
     7921 caagaaaaac taatagtaaa gtcttctcct ctgcaagagc tccatatcgc aacgggtaat
     7981 tcacgaacag ctaagacgtg gaaaaatatt acgctaactt ggcaggagct ggttgagagg
     8041 ttagagaaac ctacagtcac ccaagagacg tttgcggagt accagaagat gtctcgagca
     8101 gaaaaagggc aagcaaaaga cgtagggggc tttgtcggtg gatggctaaa gcaaggtaaa
     8161 cggaaaaatg aaaacgttca aagtaggtcc ttggttgcgc ttgacgcaga tagcccaagt
     8221 aaagatttct tagataggct agacctgctt gcggattatg cctacgtact ctacagtact
     8281 cacagccact caaaaaaagc tgctaagtac cgtcttatta tccctactga ccgtttaatg
     8341 atgcctgatg aatatgagcc agtcgctaga tacttggcta accaactagg catgtcaaac
     8401 tttgatgaca cgacttatca aagtgtacgc ttgatgttct ggccgagtca ctcaagagat
     8461 gcagacttta cgtttaaata taacgacgag gcttttctga gtgttgatga ggtgcttgac
     8521 acatacccgg actggcatga ctcaagcttc tggccagaaa gcccgacgca cgctgttaag
     8581 agacagcgcg aagctaaaaa acaaggtgac ccgcttagta aaaaaggact tattggagcc
     8641 ttttgtcgta actatgacat tagacaggcc attgcaacgt tcttacctga ggtttatgaa
     8701 gaaggaacaa ctcctgatag gtacacctac actgagggct caaccgcaaa cggcttagtt
     8761 atctatgatg atgtcttcgc ttatagtcat cacgggacag atcccgtggg ggatacgctt
     8821 gtaaatgcat acgaccttgt tcgtatccat aaattcggag agcaagatag cggggctaaa
     8881 gataatactc ctactaataa gctaccatca agcaaagcga tgaatgcttt tgtctgtgac
     8941 ttacccgaaa ttaaagacta tctaatggcg gaggctttag gcgatttcga tgaagagtta
     9001 ccagtcgaag atgacagaag ctggcttgaa attgatgaga ggggtgaacc tgaggtcaat
     9061 agttatttgc tagcaacgca gattattaag gaggttccga tttattggga cggctttgag
     9121 ttcttaagat acgacgctaa aaaaggcatc tggttgccaa atgcagagga atatttgaag
     9181 agttatatct caactaagaa actcggtaaa attactaaga ttaggcacat tagcgaaacc
     9241 attgtagcga ttaaagcaca ggctttttca agcgaagtgt ttaccgagag tgatcttaac
     9301 aagatagtgc tagcgaacgg agtctatgac ctgagggata acagctttaa gactaagttt
     9361 gatccagaat tgcatgccag gtcaagccat cccgttgtct atgaccccga ggcgacctgt
     9421 gaaacctttg agggttttct tagggagacc gtcggagctg aaaatataga tttcatcttt
     9481 gagtggtttg gctataactt ttatcgtgaa tacactattc aaaaaatgct attcatctat
     9541 ggcagcggcg gtacgggtaa atcaacactg attaatattt tacgtgaaat gataggtgct
     9601 gataattatt cagccgtgac actgcagtac ctgatgcaag aacgctttgc aaaaatcggc
     9661 ttatatcgta agacagccaa ctttgacact gacgcaaagc cccaatattt agcagatggc
     9721 gcaaccctta aaatgttgac gggagaggat actatccacg cagaccgtaa aaataaagag
     9781 ccgattaact tttacaatta tgctaagctg tcttttgcca tgaatgagct cccgcctatg
     9841 cgagatttca gcggaggact taagcgccgc atgatgatcc ttgagatgga taaagtttta
     9901 acgcaggaag ttaaggcaaa atacccccta gataagatta tgggcgaagt gtctggtatc
     9961 tttaacagag cgatggaggg gcttagaaag gccttaagta agagagattt cagtattagt
    10021 gccagcatga ggtcaagtgt cgagaaatgg gaaaaaggca acgatgtcgt agctatgttc
    10081 cttgaagacg agtgtgaacc tggtgaagac ttcaaagctc ctgttaggga cgtctaccca
    10141 gcctataaat tctattgtca ggattcaggc tacaaacctt tggcaaaaaa tgcatttaac
    10201 caccggctaa gagagctaaa ttatgaaaat aaaaacgtta aatctggtgg aaagcaagcc
    10261 aaaaattggg tcggttttag gctaaaaagt gagttttagg ggttactttt ttgggattag
    10321 agtttggtaa ccctagaaaa aagttaccaa gttaccggtt tttcaaactt taaaaataaa
    10381 aaatttaaaa ataattttta aaaaaccaaa actgaattgg aaaaatcggt aacttagtaa
    10441 ctcgaccgtt agaaatgtgt tcgaaaagta accccggtag ctttttggta gccctaagag
    10501 gcttttccta gcaatagttt agcccctatg gttaccaatc taccactttt cttctattaa
    10561 cttaaatata aatataaaaa ataaatatat ataaaataag gcatatataa aacacatata
    10621 taaaaaagag aaagtaagtt gaaaaaaaac ggtaaccctg acctttattg accaaaggag
    10681 gaaggatgag aactgaaaaa gacattgaaa attatttgaa aaagaaaaca aaaggcctgt
    10741 gtttaaaatt taccagtcca gggacggtag gagttccgga cagaattgtt gtcatgaaaa
    10801 ctggtgtatt ttttgtagag gtcaaagcgc ctggtaaaaa gccaagaccg agtcaaatcc
    10861 tgatgcacaa aaaaataaag gaggtaggtc aaccagtttg ggttgttgac tcctacgaat
    10921 cagtggacat agccttaaaa gaaatggaga actgggtgtg agactgcatg agtatcaaga
    10981 atacgctaag acatggatag tagagcaccc ttattgtggc cttttacttg acatgggcct
    11041 tggtaaaacg ctgacaacac tatcggcaat agatgagatt caaaatattt tttccgagga
    11101 tcataagatt ttaatcgtag ctcctaaaaa agtggcggaa gaaacgtggc caacggagat
    11161 tgggaaatgg cattttgatt tcacctattc taaagttttg gggagtgagg gaaaacgaat
    11221 tgaagcctta gaaacagaag ccgatatcta tttgattaat cgtgagaatg ttacttggct
    11281 tgttgaatac tacaagacta aatggccgtt tacctttgtt gttattgatg agctgtcaag
    11341 ctttaagtct agtaagtcaa aacggtttag ggctttgcga aaagttagac cgaaagtcca
    11401 acgccttgta gggctaacag gaaccccagc gcctaacagt ttgattgatt tgtggccgca
    11461 gatttatctg atggacagag gcgacaggct tgagacgagc cagactcgat ttaaagacaa
    11521 gtattttgtt cctgataagc gtaatggtcc aatcatttac agttgggcac ttagggatgg
    11581 tgcagaagca gaaatctata acaagattga ggacatctgt gtcagcatga aagctaaaga
    11641 ctatctcaag ttaccgccgc gaaccgacaa cgttgtatca gttaagttat ctaatatgaa
    11701 agcctacaaa cagtttgaag ctgatttggt gttggagttt aaaaataaag aaatatctgc
    11761 ggctaattct gcggttttgg ccaataaatt acttcaaatg gccaatggtg ctatctatga
    11821 tgatgataaa gcaacagttg ctatacacga cgacaaactt gacgcgcttg agaacatagt
    11881 agaagaaagc caaggccagc ctatcttagt tttttaccag tatcaacatg atcttgagag
    11941 acttaagaaa cgatttcctc aggctgaaga gctgacgtcg gttgacaagt ggaattccga
    12001 aaaaatacca attcttctgt gccaccctca atcggcgggg catgggctta atctacaaaa
    12061 aggcgggcat attattgttt ggtttgggct aacatggagt cttgaatatt atcagcaagc
    12121 taatgccaga ttagatagac aggggcagac agaacccgtt attgtgcacc acattgttgc
    12181 agaaaataca gttgatgaaa aagtacttag gattttacaa ggcaaagaaa aaaatcagaa
    12241 cgccttactt gaagcagtta aggcgcagtt aggagtctag atgaaaaaag aatatgtcgt
    12301 tagaatctac acgggtagag aaaagaatct tgaggcaaaa cctcagtttg aagagaaaac
    12361 ctttacgcga aaagcggaca tgttgaagtt ttggaattct tgcgaagcaa cagttaaaga
    12421 aaaatatact agggagcggg agaatgattg aagaacaata aatacttgac tgcttagtcg
    12481 acaaaatgtt tcagcaggac acaaaagcaa cttaaacgtg ctagcgagca aaatcagaga
    12541 cagacaaagc ggattgctga tttgactgga aatggggggt aggtatgaat ataatccata
    12601 gaatggaaaa cgggataaac gctgcaggtg atttaataac ttatgcagta ccatgttgtc
    12661 cagagtgtaa aagtgaaaca tatagtcaga aacactgtcc tttttgcggt gaaaatctta
    12721 tggaggtagc aaatgaaaat tgaatttttt ttaaagcaag gtcctactaa gagcgtttct
    12781 aattatggtg aagttggtat cgatgaagta attggtgcaa taaaaaataa cgatatcgtt
    12841 gcgctaaatg gaaaaaacaa cgcagttatt atacccaaac aaaatatttc tcacgtaatt
    12901 attcataaag aggtgacgaa tgaataaatt atctaacgca cagctaaagg cttttgatga
    12961 atggctgttt gattatcgtt ttattgataa caagattgca cttcgaaaac ttgagctaca
    13021 aactgatatg aatacagaag tagataaaaa tatcggtgga ggaagagcaa attttgtttc
    13081 aaaagttact gaggatgtgg ttgcaaggtg ggattcagat agggagctaa agggtctatc
    13141 caatttcaaa gaagcagtta ttgcaacgct aaatcttttg gatgaagagc taactgatat
    13201 ttttaatctt agatggggtg ttggttcaag caacacttgg gaagaaattg cctatacaag
    13261 acatctgtcg cgtaagtcta tttaccgcaa aagagagcgg attctagaaa tattctccaa
    13321 aaaaatagga atgtgacaca aaaatgtatg gaattgtcat ctttttgatg ataaattgat
    13381 agtatgagtt taaaggtaag gcaaatagtg tcttgccttt ttgttatgct tggaggtgat
    13441 ggaaaattga gtaaattaac cctaaaacag aagcgttttg cagatgagta catcatctca
    13501 gctaacgcga cagcagcggc tattaaagca gggtatagta aaaagacagc aaggtcaata
    13561 ggtcaagaaa acttgaccaa acctgacatt aaagcttata tagacgagcg gcttgaaaaa
    13621 cttgaatctg aaaagattgc tacgcaagaa gaagtcctac aatatctcac ttcaattatg
    13681 cgaggagacc aacaagaaaa gacgctcatt agcgttggag agtttggcca gaagatagtt
    13741 gacatcgatg taggtgcaaa agatagaatc aaggcagctg agctacttgg taagcgatat
    13801 agattattca ccgataaagt tgaaatggat gtgtcatcag atataactat tagcgtaggt
    13861 gagtgggatg acgattaaac agcgccctaa aattaatatc gtaatcaagc atcctagcaa
    13921 agtttttaac aaacatatct acgacaaact ttacaactat agcaacttca cggaagttca
    13981 ttatggtggg gcgtctagcg gaaagtctca tggtgttttt caaaagataa ttttaaaagc
    14041 acttaacccc aaattcaaac atcccagaaa gatattagtt ctcagaaaag ttggtgcaac
    14101 agttagggac tctgtcttcg ctgatattat gtctaatttg tcgtattttg gcgtcttgga
    14161 taaatgcaag ataaatatgt cagcttttag aataacattg ccgaatggct cggaatttat
    14221 tttcaaaggc atggataacc ctgagaagat taagtcaatt aaggggatat ctgatgtcgt
    14281 catggaagaa gctagtgagt ttactcttga cgattacaca cagcttacct tacgtcttag
    14341 ggacaagaaa catttagaga agcaagtcta tctcatgttc aatcctgtga gtaaagcaaa
    14401 ctgggtttat aaagctttct ttatcaagtc acctaagaat acagttgtct atcaaacaac
    14461 ttataaagac aaccgcttct tagatgatgt tactagagaa aatatcgagg aactagccga
    14521 taggaatgaa gcttattaca aaatatatgc tcttggccag tttgctacac ttgataaact
    14581 aatttttccc aagtatgaca agcaaatatt aaacaaagac aagttatcac acttgccttc
    14641 tttttttggt ttggactatg ggtttatcaa tgacccttcg gcatttttgc atgttaaaat
    14701 cgatgacgca aacaagaagt tatacatctt agaggaatat gtcagaaaaa atttgaccaa
    14761 tgataaaata gcaaatgcta taaaggacct tggctatgcc aaagaagaaa tcagaggaga
    14821 ttcggctgaa aagaaatcta accaagagct gaggaattta ggtattccta gaatgattga
    14881 tgttaccaaa ggtcctggaa ccgttatgca aggaattcag tacctgcttc agtatgattg
    14941 gattgttgat gaaaggtgtg tcaagactat tgaagaactc gaaaattaca cttggaagaa
    15001 agacaagaag actaatgagt atatcaatga accagttgac agttacaacc actgcattga
    15061 cgccataaga tatgccgtac aagacagaat atatcagtcg gcagatagaa gtaagcgcat
    15121 gaagaacgcg aagtactatt tttaggaatg aggtgagact ttggaagaaa aacaattttt
    15181 agtaggaact cgttttaatg agaatgctaa taagcaattt attatgctgc aagaagactt
    15241 tgaagcaatt gactatgaat caaaactttg gatagatcaa cttaagaatt acatttcaag
    15301 atttaaagca gaacaattag agcgtttgaa agagttaaag cgatactatc ttggggataa
    15361 taacatcaag tatagacctg ctaaaacaga taaatacgca gcggataatc gtattgcaag
    15421 tgattttgct aagtatatta cggtatttga gcaaggttat atgcttggcg ttcccgttga
    15481 gtacaaaaat gaaaataaag acctccaggc agctattgac cttatgtcgg tcagaaacaa
    15541 tgaggattac cacaacgtca aaataaaaac agatttatca atttatggaa gagcctacga
    15601 gctgttaact gttgaggaaa tagatgacaa aaaaaccgaa gtaaaactct accagctccc
    15661 agccgagcaa acttttgtta tctatgatga tacataccag cgaaattcgt tgatggccgt
    15721 tcatttttac gacatagatt atggctcagg taaacgcaaa cagattatca aagtatatac
    15781 ttccgacaca atttatacct acgaagacta taatttcgaa acaaaaggta tgcgcttaaa
    15841 agagcatgaa gaacattttt ttaaaggtgt accagtcaat gaatatgcta ataatgagga
    15901 gcgtacaggg gcttatgagt ctgtacttga taatattgat gcctatgatt tatctcagtc
    15961 agagcttgct aattttcaac aagattcagt caatgcgttg cttgtaatag ctggtaatac
    16021 ttacacaggg gctgatgaga atgactactt agatgacgga cgattaaatc ctaatggtcg
    16081 tttagcgatc tcaattgggt ttaaaaaagc ccaagtgtta attttagacg ataatcctaa
    16141 tccaaatggc gttaaaccac aagcgtactt tctcaaaaaa gagtatgata ccgccggtag
    16201 cgaagcctac aaaaacagac tagttgcaga cattttgaga tttactttta cgccagatac
    16261 acaagatatg aaattttcag gagttcaatc tggtgaatca atgaagtata agcttatggc
    16321 ttctgacaac tatcgcgaga agcaagagag gttgtttaaa aaaggcttaa tgcgacgctt
    16381 acgcttagca gctaatatct gggccatcaa agggaatgaa gcaactacat atagccttgt
    16441 aaatgatacg agtatagttt tcacacctaa tcttcctcaa aacgataatg aaattgttac
    16501 agctgcacaa aatctctatg gcattgttag cgatcagact atctttgaaa tcttaaatac
    16561 tgtcacagga gtagacgctg aagcagagct tgaacgtttg aaagaagaag ctgataaaaa
    16621 gcaatcttta ccggagccta gattggtagg tgatgctagt ggtcaagaag aaccaacagc
    16681 agaaaagcct taattactgg caaaaacgtc aagaagatat tctaagttat ttagacagaa
    16741 ctgatttaga cgtcttttca gaactgcaaa agttatataa tgaacaggct tttgagttac
    16801 aaaaggaatt gtttgatttt tatactaagt attctgaaga gaacaaaatg acttatcagg
    16861 atgtcgttaa aaagctacgt catgaagatt tatcagatta cgtagctaat gctaacaagt
    16921 atcgtaaaca agctgagaaa gaccctgagt tgctaaaacg acttaatgag cagtatgtat
    16981 cagcaagagc tacaaggatg gatgcattaa atcttgaact tgtttatcgc gcagggatac
    17041 taaaaggtgt acttgattca gtatttgaaa atcatttaaa aaaagctgcc tcatatgctt
    17101 ataaaaaagc aatgggtgga cggtcaggga caatcaatgg tccagtttta gaagaactgg
    17161 ttagaacccc gtttgatggt tataactact cagaacagtt gtggggcaat actgacaatc
    17221 ttgtcaagaa tctccaaaag aaattaaaac aaggttttgt tcgtggcgag catcctagag
    17281 cgatagccag agatttagct aagcaattta atgttgctaa tcacagggct gaaacactaa
    17341 tcagaactga tggaactatg gtgattaata atgctaccgc taggcgctac ttgaatgcag
    17401 gacttaagta ttatcgtgat ttagttaggc ttgatgacag gacaactgag atatgtcgta
    17461 aaatcgctaa agaaaacaaa agaaagctat tatctgagct aaagcctgga attaatgcag
    17521 caccctatca tttcaactgc agaacaacca ttattcctga tgaagatgag ctaagcattg
    17581 aagtagatcc aattgatgat aaaagcacta agtattttaa ggatgtcacc tcagattgga
    17641 tagatggaaa tgagcataaa ccacaactgt cactactaaa tgagtatgta aaaaatggca
    17701 ctccttacaa agtagatggc cataatgttg ttcttgatca ctcgaattat gaatatagag
    17761 ttgctaattg gttatctaaa aagacaggat tgcaagttga catggtgcca agagttaatt
    17821 cccctgaaca tatcaatact cctgattatt tagttgatgg agttcctttt gatctaaaag
    17881 aaatcacagg ttcgggtaaa aacgtcattg atggcaatct acgaaaagca aaaaagcagg
    17941 caactaatat catttttgat ataacaaaaa cccctttatc atttgaagag ataatggggc
    18001 agttagaaca catttatatg ttagatcgta ggggtcttga tatatcaatt atcaaaaata
    18061 aagatgaggt attagctgta ttagaaaaag aaggacgatg acccaccgcc tccacagtaa
    18121 actgcttcat gggcgttaga ccatcgtcct tctttatctc aattatacat caacctatta
    18181 aaactatcaa ggaggaaaat atgtttattt ggcaaatggt attagtggca ctaggtgtat
    18241 tggtgctcat attgattgtt ggcattgcag caatagcggt taagtcaatt attgcagaat
    18301 tgaaaaaggt aggataaaca tgaataagcg cattaagaaa aaacgtaaat tggaaacagc
    18361 ggttgtgatg cttgttgcag agaatgccat gcaagcagaa gctcttagaa atcaaaacaa
    18421 acaaattaca gagctgagag cgattgtaca acaaaacgcc caagctacca atgaagagtt
    18481 tgcgtctgtt aaagctgcta ctttagataa ccaatcagct attgcagata ttgcaattga
    18541 cgttaactat attaagaaaa attacaagcg aaaatgggga aaataaattt taaacgaatt
    18601 cgaccccttt agaaatcaaa gtcgtagcga tgcggccttt tattatgtcc aaactttgtt
    18661 gatgacacta aaagctacac tgtttcgtcg caggacgtaa aactagatta tcggttggtg
    18721 gcgtaaccac taggagaaat tatggcagaa gaaaatgtaa caacagaaac tactgaacaa
    18781 gtcgacactc agcaagaaac tgttgaacat cctaagcagg aacatgagcg aacttttact
    18841 cgcgcagata tctcaaaaat gatgactgct gaacgtgcaa agtgggaagc agagcaatca
    18901 gaagcaatcg aaaaagctcg tactgagggt gaaagactag ctaaattatc aaaagatgaa
    18961 cgtgctaaag aagaagaaca aaaacgtctc agtgctattg cagaacgtga aaaagcagtt
    19021 gcagaacgtg aaatgcgcat cgaaacacaa tcattacttg ctgaaaaagg attgccacta
    19081 gattttattg atgttgtttt agctactact gcagatgaag tgaaaacaaa cattgataat
    19141 ttacagtcta tctttgatca agcagtggaa aatcgtgtta acgaaagatt aacgcaaaag
    19201 tcacctcgaa ctggtaatgg ctctgtcggt atgactaaag ctgaaattat ggctattgag
    19261 aatgatgatg aacgtctcaa agctattgca gctaatcgta atttatttac aaggtaagaa
    19321 aggacatatt aatttatggc agaaaataat ttaaatgtga tgtcagactt aggagaaatc
    19381 aaatctattg attttgttaa taagttttca acgaatatca atgaactact caagcttttg
    19441 ggagtaactc gcaaacagga attgactaat gatctaaaaa tccaaactta taagtggtct
    19501 actactttag ataagacaaa tcccgccgag ggtgaaacaa ttcctttatc taaagttact
    19561 cgtgcaaaag ctaaggaata tactgttgag tggttcaaaa aacgtcgtgc aactaccgct
    19621 gaagcaattg cacgtcatgg tgtagctcgt gcgattaacg aagcagatac acgcattatg
    19681 cgagaaattc aaaatggttt taaaactaac ttctttgaat ttttaaagaa gtcaactaca
    19741 aaagtgactg gaacaactct tcaaagtgca ttatcacaat catgggcaaa aattgctact
    19801 tttaatgaat tcgatggttc acctatcatc tctttcgttt ctccacttga tgttgctaag
    19861 tatctaggag atacaaatgt aggtgcagat gcttctaacg tgtttggaat gacattgctt
    19921 aaaaacttcc taggtatgca aaacgttatc gttttaccat cagtgccaga gggaaaagta
    19981 tacacaacag ctgttgaaaa ccttgtgttc gcttatttaa acgtcaatag tggcgatctt
    20041 ggagggatgt ttgctgattt tactgatgaa actggattga ttggtgcggc tagaactcgt
    20101 caactttcta atttgaccta tgaatcagta ttttttggtg caaatgtgct atttgcagaa
    20161 attccagaag gcgttgtaga agcaacaatt gaggctccaa aagtagttcc aggaggctaa
    20221 tagatggata aggagttatt actcacagag gttaagctat tcaaagggat cagcgccaaa
    20281 gacactagcc aagatgattt gataaaatta tccattaatg aaagtattga ccgtatcctt
    20341 gcgaaactca atgattactc agacacagaa atgactgaaa ttcctaagcg tcttactttt
    20401 attgtcagag atgtggctat taaaaggtat aacagactta attcagaagg aaccgttgaa
    20461 gatagcgaag aaggaaagac ttttaagtgg gacagttacc ttaaagagta tgaatcaaca
    20521 ctcagaagcg ctgctattgg gaaggtatat tcaggcaaag gggtagcaag atttatttag
    20581 gaggtcataa atgatttaca aagatagagt tatcctagtg tatgtcgatg agcaggacga
    20641 ttttctagat aaaagaactg ttgaaaaacc tagcggaaaa atcccctgca tggaaaatac
    20701 tttcacaaaa tctgaacaga tggggttatt tggtaaatat gatttgaacg cttttaagtt
    20761 gcacttgcaa ggccgatacg atggctttag caagattatc tacaagggga aaccgaggtt
    20821 gataaaggga ctagcgcacc ataaaaatag cacggttatt tatgtatgag tcttatttat
    20881 cggatgagag gcctagatag gtttttacgc agcgttgagc gcaagcagaa gtcagtacga
    20941 atcgctgtag ataaagagct tagtaaatca gctgctagga ttgagagaca ggctaaaata
    21001 ctagccccgg ttgacactgg atggctgaga gctcaaatct acagtgagca acaacgactc
    21061 ttacactaca gagtggtttc tcctgcttta tattctattt atcttgagtt aggtactcgt
    21121 aaaatggaag cccaaccatt tttagaccct gccctgagaa aagaatggcc agtgttaatg
    21181 gctaatctca aaaaaatgtt taagaggtga tgcatggatt actcaccaga aacactatat
    21241 ttaaaaaagg taaaaaatag attgggagtt ttagacatac caatctattt taaattgcct
    21301 aaatcagacg ttttagagcc ttttattgtt gtaggtacaa atatatcaga cttgtcaaaa
    21361 acagctcaaa ctggagcagt tattgatgat tttagcctga atatcgatgc tttcttacct
    21421 ggcgatagtc gtttggatgc agaagagata aaatctcgca tgcttagact gcttgggcgg
    21481 aataaccaaa taaaggctca gattttggta gataattcaa taggacgaga agtctataga
    21541 gttgctatca acattacaga aatactaatt taaaggagaa ttaaatggaa actacagttg
    21601 aaattactac agctaagcct attaaaggta aaaatatatt ttattttatt caatctgttg
    21661 atgctgaatt aggtagtaaa gcaatactgc cagcgtatcg tacagatgga acaacgaagc
    21721 tcggtggaga ttttatcgat gaacaaactc aacaaggacg tgtgcttgaa aaagcaacag
    21781 atgagcactc aattgatttg acatcatact acacgccgaa agacaaagca attgaaattg
    21841 tagaaatagc taaaacaact ggtaaatctg ttaaagtttg gcgtgttatc gcagacgaat
    21901 ctttgaaaga aaaaggttca ggaactttag agggtaaaga agtttatccc gcgaagtttg
    21961 gatatggaaa aattgacgag attgaatatt cagaaggtgt tgaaaaactt gtagaagcat
    22021 catatacatt gaatattatc gatagtttaa aagatggcaa atttccactg acggcagaag
    22081 atatcgcttt gttaacaagt gtttatgcat atcaaaaccc aggcgaaact actggcgatt
    22141 atgacaatat ccagaaaaaa gaagtttcag gaagtttact gggagcagtt ccagggatat
    22201 catcagaagg acactaattt ataaggtagc atttcgctac cttttatttt taaggagaaa
    22261 tacataaatg gaattaacag tagcaaaacg cagtattgaa attaaatttg attttaaaac
    22321 aatgtttaag ataaataaca gacttggaac tgtgaataaa gagacagggg aacgtaacgc
    22381 tgacggcgtc ggtacgctat tttataaaat cttagagcgt gatgattcag ctgtagtaga
    22441 tttagtcaag ctgtctgtag gcagcggtaa aaaagcattg tcagaagatg aagtacttga
    22501 ttctatcgca gagctagtcg aagaagaagg atcgacagaa gccttattta aagaaattga
    22561 gaatgacatg gttgaatctg gttttttcaa agagaagatt tcgaaatata tcgaaagcat
    22621 ggagaaatca gtgaaatatt tagaagctcg agaagatgcg gacgaagctc aaatccaaat
    22681 cgtaaaagac atgattggga aaatgaaaga cgcaatctct taattaactg tgcccaactt
    22741 ggcctgacgg acattgatat tatttacaag tgtacgagaa cagaattaga tgcaattatg
    22801 gaaggattgc agtacaagca aattgcagaa cgtgaaaacc tatctgaact tgctttaaat
    22861 ctcagataca cgttaaatgc taaaaacgtc aatgctaaca agctcaaatt agataaacaa
    22921 cgtaacaaaa ttaaacgaac ttatcaaaat aaacaagatc taactaacgt ttcaaatgat
    22981 ttcgcagaac gtgtggcgaa gctcaattta cattttcaaa atttataaca agaaaggagg
    23041 gattttatgg caggtacatt tgatgggtct atattcgctg atattggtgc aaatactaaa
    23101 gattatgagg cagctatggc tagaattgtt agtactacac aaaatgcttt tagaaaagcg
    23161 caggatactg cagttaacaa cagcaacaaa atgatacaga tgattggtca aattatggct
    23221 caattagcaa ataatggtca atcattaggg caaagactcg gtaatgcata tagtacaggt
    23281 ctcaagttat ctcttggtca aattcaaagg atagcagcat ctatcggtga aaaaattccc
    23341 gaacccataa agaatgggtt caataaagct ttttcaatta ttcaaaatag cgtacagaag
    23401 ttatcgagcg ccatccctca acccataaaa tccgcattca caagtgcaac aagcgcagta
    23461 gttagctttt cgtctaagat cacaagtgcc gtctcagctg ctttcaactc tgttagttct
    23521 aaagcgagta ctgttgcaga taaaatcagt agtagttttg ggggtagaat cacttcagcg
    23581 gttactaatt tagcgactaa gttaagcaca ggtcttagca atggttttag caggatgtcc
    23641 agttctgcaa ctagtgccct aaatggtatt agtcagagat tcgcaagcgc atcatctgct
    23701 ggtgaaaaac taagaagcac tgtgggaagt atcatttctg cttttagttt gatggccatt
    23761 gctcggaaag gaatccaagc aatcacagga gcaatggacg gtgcaataag tcgtgtagat
    23821 accatgaacc gctttccgaa aacgatggcg ctatttgggt attctgcaca acagtctaaa
    23881 gcatcaatag ataaattatc taaaggaatt gaaggacttc ctacaccact agacagtgct
    23941 gttaaaagtg cccaacaatt agctataact acaggtagct tagataaagg aacagagcta
    24001 gcattagcat ttaataatgc aatgattggt tatggagcga ctactgaagg ggctgagcaa
    24061 gcacttagac agtttaacca gtcgttgggg tctggtaaga tatatgccga agagtttaat
    24121 tctgtgtctg aagctgcccc tggcttaatg tctaaaatgg cagagtcatt cggatttggt
    24181 aaaaatggtg ttcaggactt aaaagcagct ctttcagacg gcagtattac tgcccaagaa
    24241 ttcgctgata aaatgattga actaaacaag gcgcagggtg gttttgctga gatggcccaa
    24301 tcatcggctg gtggaatccg aactgcatgg aagaacgtac ataccgccgt cgtaaaaggc
    24361 gtagcaggta tgatttcagc ctttgatgaa gctgccaaag ctaatggtat gaagaccatt
    24421 gcagaatcca ttaatagtat taagccagct ataaattctg catttgcaac tgtgaactca
    24481 ataatgccaa accttgttgc agcattcgct agaatctcca agatggtgaa tattgacttt
    24541 agtccactaa ctagtggaat taaatcagct tttggattat taaatactgc tttaggtgaa
    24601 tttgcaaaaa ctggtgaagt atcgtcagca actattgata gaatcaaatc aaagatagca
    24661 tctataggtc ctactgcaat agcagcttgg gccttattaa atcctgcaag tgctattgca
    24721 actattatgc ctttaatatc aattattggt aagattggtg ttgctcttgg gactgttggt
    24781 tcagcgttgg gatcattcgg agaaatggta gccagtggat tgtcaactgc tagtgggcta
    24841 gttggagctt ttggtagagc tatgtcagga cttcctggaa tatttggtgt agctgcaagt
    24901 agaggtcttg ctgtacttga tggaatgaca actgcgatta gtagtgctgc aatactggca
    24961 atgtctgcta taggtcctgc agcaatttta ggattggtcg tagtaggtct tggtttagta
    25021 aacaatcaat ttggtactca aattgataaa ttgataaata cagcaattac taaaggacct
    25081 ggtattattc aaggccttgt aaaaggcatt acttctaaaa taccagcttt gatagctagt
    25141 ggtactcaat tgattgctaa gtttgctaat gctctaacag tattattgcc agttattatt
    25201 caggcaggtg ttcaattaat taccagtctg gtgcaaggta ttggtcaaaa cgcaggtagt
    25261 ttaattagtt cggctcttaa agttattgga acttttgtaa gttcaattgc tagtgctctc
    25321 cctcaaatac tagctgtagg tgttcaatta atactgaact tagctcgagg tattgtacag
    25381 aatatacctt taataatcca gtatgctcaa caaattattt ctaattttgg ttcaagctta
    25441 caagctaata tgccaagcat aattagcaac ggtatagcaa ttagcaccaa tttgaatgct
    25501 ggtattatcc aaatgttgcc ggcagttata caaattgcaa cccaagtcat cacaggtttt
    25561 atttccggta tagctcagta tttgccacag gtaatccaag gcggcatcca gattataatc
    25621 atgttagtgc aaggtattat tcaaaattta ccgcaggtaa tccaagcagc gatgcagatt
    25681 attcagtcat tgatatctgg tttatcacaa gcattaccac aaattatatc tgcagggata
    25741 cagttagttg tgcagttggc tgttgcaatt attaaagggt tgccgcagat tatttcagct
    25801 ggtattcaat tgattgcggg tcttggtaaa gctatgcttg aggctattcc gaatgctctt
    25861 tcaggggtat gggaaggcat taaaggtgga tttagttcta tgtgggacca aatcacagga
    25921 aagagctcaa caagtgccgc taaggttaca tcagacatgt caacaatgac tatgaatgtt
    25981 ggtatgcaga cgaccacaat ggctaatcaa actaacatag acacaactac tatgctaaat
    26041 agcattagtc aaaatactgg tcttgctaat ttaaatgcga caacaaatgc tactcagatg
    26101 gcaacaaatg taaatgcaca aacagcatct atggcaaatc aaggtttgct gaatgcggca
    26161 ggatttaatg aagcgttgag ttcgaacatg actttagcaa atacaaatgc tactaatcaa
    26221 gctaatcaaa tgactgctaa tgttaataac gctactagcc aaatgagtct gggttctcta
    26281 ccgaatttct tggaattatc gcaacaggca acaagtaata tgcaaactgc acaagcaaat
    26341 gctaccactt ccgcgaatgg aatgagttct ggagttactg gtgctacagc tggtatggct
    26401 agtggtgtag ctaataacat gttaggtata actagcactg tatctacagg gatgaatcaa
    26461 gctgcaaatg ctgcaaaatc aggcggtgca aacatatcta atagtgtgaa tagcagtttt
    26521 acacaagcca aatctacagt ttcaagttca atgtcaggaa tgaacagcac tgttactagt
    26581 ggaatgaata gtattaataa tactgtaaaa tcaggtggaa ataatattaa atccaccttt
    26641 acaagttcct ttaattctgc taagtctaca gtttcaaatg ctatgtctag tattcgttcg
    26701 tcaatttcaa gtggaatgaa cagcgctgtg agtgtaacaa gaagtgcaat gaattcggtt
    26761 gtatctgcaa tgaatgcagc agcaagtggt gctagatctg caggttataa tactgggatt
    26821 ggatttgcaa atggcctagc aagcactgca ggaactatta tggctattgc taatagtatt
    26881 gctgctaatg tcgctgcaac aatcagaagc gcaatggata ttcattcgcc ttcgcgagta
    26941 acgcgagaac ttggtgctta tactggtgaa ggttttgcac ttggtatgca ggattggatg
    27001 gatgatatca atgcaatagg taaagagtac gctaatgctg tgactgatca gcgatgggaa
    27061 acagattcac aattagcgac atctgcaaac atatcaagtg caggtgtatc atcatcactt
    27121 gataatctaa gtgacgaggt acgcaaccaa caattgacta gtccaacgtt cgaggttcat
    27181 aatgaacttg ttggtgatga aatttataca actattaaat ctaaagaagc aagagaaagt
    27241 aataaagata attacttcaa ctttgcgaca taagagagga gaaaaatgga tttattagta
    27301 caaaagagtg atcaatcctt tcgattttca gagattggtc tacgaatact tgatgtcgat
    27361 gaccgttcat catctcttga ggttgatagt cgaacagtga aaggtcgaag cggtaaaatc
    27421 tttgctagtg ctaagtatgg cacaaaaaaa gttaaggtta atgggcgctt agttgtagct
    27481 tctattaaag attttatgac aaaaaaagat gatgtgaatg gcttactagt ggatagtgag
    27541 cctttttaca ttactaaaat gtatcctcag aaagaaaatt tatatgattt cgaattgcct
    27601 gggatgaaag caggtgattt agacttatta aatcaatctt atacagcgtg gcactatcgt
    27661 tggaaagttt atgtctcaag tgaacctgag tacacttttg ttggtaagtc tagtgaaggt
    27721 ctcaaataca actttacaat tgcatttgag actgcagaac ttccatttgg tgaaactatt
    27781 gcacaaaata tagtcatcac taacggaacg tttaattatt ctggtactgc accttttagc
    27841 cagttagaat acccatggtc tgtagagtta acttcaactg gtggacaaag tagtttttat
    27901 cttgaaatcg atgggaggcg ctgggcttat caatatagtg caccacttaa tcaaggcgac
    27961 gttattgtaa tcactggtat atccacaaca ttgaataaga taaatgtgac tgctagaacc
    28021 aatcatgaat attttgtttt aaagcaaaaa gtcaacaaga ctattctcta cagcacaaat
    28081 tttcaaggca caatcaagtt tttaaacttt aaagaacttt acaagtaagg aagtgataga
    28141 ttgacaactt ttttagatca taaagataat gagtttgaag ctcaagctgt tgttaaaaca
    28201 actaatgcag ttaacggaga aaggtcttta tcaggaacaa tttatactaa cgaagaggta
    28261 ctaaacaagg tcgataaagg ttggaaactt gagtttgatg atgagtatta caagattatt
    28321 tatgctaaac ctactgatac tggtaacaaa attcaagttg aatttgatgc agttcatcaa
    28381 tttttttatg attttgataa atcatcacta cataagcaat taaatgatgg ttcacatact
    28441 ttccaagcat atttgaactt tatttttgat ggcagcgggt atacttacag tttagaagta
    28501 gttgtcaaat ctttcgaaaa gcaatcgttt ggatataaat cgcgacttga tttatttaat
    28561 gacatcatta agagtgcagg agttgaattt tttgttcgtg gcaaggttgt tcgaattcta
    28621 caaaaaacag gaacagattt atcaacaatc gtacgtaaaa atttcaatat gaatgaaatc
    28681 ataattgaaa agaaaatcaa tgattttatt acatatcaga agggttttgg agcgtgggct
    28741 gaccaagagg atcattccaa aggaaggctc gaagtagaat atgaaagtcc acttgcaaag
    28801 gaatatggac gaattgaggg tgagcctgtt gttgatgagc ggtacactaa cgctgacaat
    28861 ttaaaatcag tactaaaaca aaatgtagat tcgtcttaca atatcgctgt aaaaattgac
    28921 attgaagatc ttacaaaagc aggttatcag tacactcaac ctattgccgg tgattatatt
    28981 atggctatca atgagacact aggatttaag gaaaaaatcc gaatagtatc gtttgaaagt
    29041 gagtatgatg taacaggtca gctgattaac cataaagtca cttgtaacga tattggtaca
    29101 atcaaaaaac aaactgtagc tgttagccaa ttatcaagta agattaataa taaccaagca
    29161 atactagata atgcagtatt taaagcaaat caagcactag catcagctga tggtaaaaac
    29221 actaactact atggtacgga gatgccagta gataatccaa aaggcacgct aagaaagggt
    29281 gatttgcttt ttttaactgt tggcgatact accagaatgt atttctggaa tggtgctgaa
    29341 tggattatca attcttttag cgacgatatt gattttgtaa agaaagaaat tactaataaa
    29401 atcatagagg ttaatgcagc aatgaaactt gctgatgaac agttaaccga aaaatataat
    29461 gcaattgttg ctaaaaatgt tagccaagac gaattgatca atcaagctaa aactttatct
    29521 gactctgcaa aaaaagaggc tacagaagca ttaacaaatc ttattagtga atctaaaaaa
    29581 ctgactgacc agatgtctgc gttatctaaa acagaagttg agcattattc aacgactaat
    29641 cagcaactga ctcaaattga tggtacagtc aagggtttac aaactagcta tgaagcattg
    29701 ttaaaaaaag atggagatat cactcaggca cttgctaatt ataagcaaac aattgaccaa
    29761 aacagtgcaa gtattatgac caacaaaaag actgccgata atgcacttag cagtctacag
    29821 acacaagtca tacaaacgtc gaacgaaatt acaacgagat tatctcaaac aaatattgaa
    29881 gcgttgatta caagcaagtc tgccgttatc gctgataata aagtcaagga aactgcagat
    29941 agttttagta gagaattaac tcgtgttaat aatactattg ataatttaaa gataggtaca
    30001 gttaatctac tatctggtac taaaggttgg actaaaaatt ggtttaatcg taacaactgg
    30061 caagctgatg atgacagctt gtctattggt aatttcaact taacagtgct aaagcgtaag
    30121 atgatgtgga gtggtatatc acaatcatat gctgtaaaaa gcggagagag ttatgttttt
    30181 agtgcatatg ctaaatcaag catagataat gatgttgtta atttatattt gttaccaaat
    30241 gatcaatcgc aacaggctaa gataagtatt agtttcaagt cgatcacttt aaacacggat
    30301 tggcaacgta tcgttattaa atttaacgta aattctgatg gctatatttt accacgcttc
    30361 gaacgcttta atgaaaatgg ttggttgtat attgcaggtt accagcttga acatggaaat
    30421 gttacaagtg actattcaga gtccccagcg gatcaagaag ataaaatgac tgttgaattt
    30481 aataaaatca acgatacagt taatagtcat agtcaactga ttggtaaaca aaacgagagt
    30541 ctgacagcta caatacaaaa agttgataat atacaatcaa cagtatctaa tgtggatgga
    30601 cgcttgtcac gagttaccca aacggctgac ggtatagttg caacagttag tcagcttgat
    30661 aacaacattt tacctggcac aaaaaaattc acaggatggc aaacagaagg tgcagtacta
    30721 gaggaactaa cacaatcgcc ttacccattt gtttttaaaa aatgggtaag cggtaataag
    30781 gtgtcacctt taatcgaata tgatgtcaaa aaagatcaag agtatacgtt tactgcatac
    30841 atttctagag aacaagcagg aaatctatat ttctacttgt atgatctttg gaatcatcat
    30901 attacaagca caactcctcg tgaaactatt attaaagacg tcactcaaac cattagacga
    30961 tttaaaataa catttattcc aactcgagac ggtaaaatta gaccacgctt tgccatgctt
    31021 gctagtgatc caggtagctt tagtaccggt ggttttatgt tatctaaagg tacagttgag
    31081 ttggaatggt ctgaatctaa gcaagatatt aaagatagtg tgactgctat taatacaaca
    31141 gttacacaaa cggctaacgg ttgggctatt aaaaatttaa cttctagcgg tactgtgttg
    31201 agtcagctta atttgacgga cggtaatgta aaactggaag gtaagttaat acacttgtca
    31261 ggacagtcta ttatcgacaa tggcattatt actaatgcga tgattaaaga tttaactgca
    31321 gataagatta cagcaggtac aattgatgct aatcgtataa atgtcattaa tttagatgct
    31381 agcaacatca ctagtggcag gatgtcagca gatctgatac gtagcggcgt tttaatatcg
    31441 caaaacggtg caatgttagc agatcttaat acaggacaaa ttgaatttta cacagatagt
    31501 ccagctatca aacgtattac tgcaggttat cctaaccaat tcgttaaatt tgcaacaggt
    31561 aatgttgaag gaaagggcgc ggctggcgta acggttatag gttctaatcg atggggaagt
    31621 gaatcgtcaa acgacggtgg tttcgtaggt attcgcgctt ggaatggtaa agacatcgat
    31681 actcttgatt tagttggcga tagtattcac ttagcaagct ctgcgtacac taacgctgat
    31741 ggttgggaaa tcattacttt gccaaatcag ctatcaatag atgcacgtaa cataaatcat
    31801 cgtgtaacgt ctaagataaa agtaggagat atttggctat ggaaaaatac atcaacttat
    31861 acaagcatga aagatacaat taatatgatt attgataact tgcagctact acacaataat
    31921 aaaaccactg aaaaaggcta cagttatact attccaggaa agatttagga gaaaaaatgg
    31981 aagaaaaaat aaaatcaaat ttagctttac aaatagcgca attatcttta gataaagcaa
    32041 ctctacaagc tcaaaatgag caattacaat tacaaattgc tgagttaaat cagcaattag
    32101 atgagataac taagccagga gcaaagtgag gtgagtaaca tgaaacaatg gaacattgtt
    32161 ggaaaaccat ttccaatcta tcaagatgga gtggttctaa aaacggatgt acgcttacaa
    32221 ggcgacgatg gtacatattt accacaagag ctaattggta atcatgttga taaggataat
    32281 caatcactga taaaacttgt acttgaaact tttgcaaaag aaaatgtcgt tgatttcgca
    32341 atgcttgagt ctgtaaaaga tattgagcaa ctaaaagctg acaaagtaac tttaatcaaa
    32401 aaaattgcag aggctgatga ggcaatttct aagatgtcgc aagcaacaaa agatatgcag
    32461 cttaaagttg ataaagcagt tgtcgagtta actgacttag tgacatcaag tgtatctagc
    32521 ctttccgtgc cgtctacatc gatggaatca agctaatgga actaacgttg aataccgtta
    32581 taacactata ttatttaata aaaaaaggag aatttaatat gacatatact tttaaaaaag
    32641 atcatcttat tgttgacgtt tggtaccgtc gtgtcgcgaa tggtatttgt gatttcgaag
    32701 cagtacctaa attatttaat ttacgagatg ttgtgcttga gttacttagt caaaaagtta
    32761 ctgataaaga atcagagtga ggtaacttaa tgatagattt tgtgcaagtt ggcgcttttg
    32821 caggtgctgc tttatctatt ttgggagtat ggggatttat tgttaatccc tttaaaaaag
    32881 caatggaagc taacgagatt gctatggctc agctcaagga ctcactcaaa gaactagcat
    32941 acgagctcaa aaatatagat agagaccgag agattacaaa agctgttatt gatcgtcacg
    33001 aggagcgttt aggtcgtgtc gaggatgata tcatcatcaa taaagagcaa atcaaaacat
    33061 tatttaataa ggagaaataa gatgattaat tggaaagtaa gaattaaaaa caaagcattt
    33121 tggtcagcaa ttattccagc aatattttta gttgtacaag cagttgcaaa tgtttttggt
    33181 tatacacttg aacttagtga tttaggtaat aaattgttag tagttgttaa tagtgggttt
    33241 tcagtacttg ttattgcagg tattgttaca gaccctacta cacaaggagt atcagatagc
    33301 agccgtgcgc tatcctacac agagccaaaa taataggagg ataaaatgaa agcaatcact
    33361 aaaatagcaa tggtactagc aatagcaata ctgtacatac cgcttgcagt ggttgctttt
    33421 tttagttatc cgatttattt actttttgga aaggaggagt aaatggcaac ttatcaagaa
    33481 tataaaagca ggtcaaatgg taacgcttat gatattgatg gttcgttcgg tgcacaatgc
    33541 tgggacggtt atgcagatta ctgtggattt ttaggtgtac cgtacgcaaa ctgcacaaat
    33601 acaggatacg caagagacat ctgggaacag cgccacgaaa atggtatctt aaactacttt
    33661 ggcgaagtag agactatgca agcgggagat gtcgcgattt ttatggttgt tgacggtgta
    33721 acaccttaca gtcatgtagc aatttttgac agcgatgcgg gtggaggcta tggttggttt
    33781 ttaggacaaa accaaggtgg accaaacgga gcatataatt tagtaaaaat accatattca
    33841 gcgacttatc ctactgcatt tagaccaaaa gtttttaaaa atgcagttac tgttacaggt
    33901 aatactggct taaataaagg tgattatttt atcgatgtat cagcttatca gcaagcagat
    33961 ttaacaacga cttgtcagca ggcaggtact acaaaaacga ttatcaaggt atcggagtca
    34021 atagcgtggc tatctgacag acatcaacaa caagcaaaca caagcgaccc aattggctat
    34081 taccactttg gacgttttgg cggtgatagt gctttagcgc aacgagaagc agacttattt
    34141 ttgtctaact taccaagcaa aaaagtatca tacttagtca ttgactatga agattccgca
    34201 agcgcagaca agcaagctaa cacaaatgca gttattgcat ttatggataa aatcgctagc
    34261 gctggttaca agcctgttta ctacagctat aagccattta cacttaataa tgttgattat
    34321 cagcagatta ttgctaaata cccaaatagt atttggattg caggctatcc cgattacgag
    34381 gtgcgctctg aaccactgtg ggactatttt ccatctatgg acggcgtgcg ctggtggcaa
    34441 tttacaagtg taggagtagc aggtggttta gataaaaata ttgtattatt agcagatgat
    34501 agtagcaaag tggatatacc taagattgac aaaccacaag aaccacaaag ccagcttact
    34561 tttaatcaaa agctagatac taacactaaa ttagacaact cgaatgtacc ttactacgaa
    34621 gcgactctaa gcacagatta ttacgtagaa tctaaaccaa acgcaagtag tacagataaa
    34681 gagtttatca aagcaggtac tcgtgtgaga gtttacgaaa aaatcaacgg ttggtcacgt
    34741 attaacgcac cacaatccga acaatgggta gaagatgctt acttaattga tgcaacagat
    34801 atgtaaacca acagagcgac ataaatgtcg gtctgttaat ggtgtaagtt acaccgcaac
    34861 taaaaaacca gtttaggagg tattatttta ttgttaataa taaatattac tgtttttctt
    34921 gacttagcaa ataaaaacag gtaatataaa gatacaaata acgacaaaaa actgcgcctc
    34981 tgttgttttg tgctcttgtt gtttgtattg ctgtacacct atcgccgtag gtgacgaaca
    35041 aaaaatgtaa gaggggactc caacctcaga aaagcacagt ttgccggctg tgcttttatt
    35101 ttttgaacat aaaaaagagc gggtttgccg acccactctt aaacaaaaaa tgtaatgtac
    35161 gtactatttt ttgtgtaaat agtcacgtac gatatcagct aagcaattcg cagtgagagt
    35221 aaccacaaaa ttgctaacta atgtgaggag gaaaaattcc atactccaac ctccttttta
    35281 aagatttgct atttgattag ttagctctag cccatctaag ctactagagc ttatttgttg
    35341 attttattat atctgatttt gcaattttaa aaaaggtctt agctgctaaa aaaaacaaga
    35401 acttctagta ttataagaca aaaccgctca gattaacttc tgggctgttt tttattgcaa
    35461 aaaatttcgc atttattgac aaaaagttca agacatgtca taatgagggt ggttatagaa
    35521 gataaatttc gttcatttat catcatttct aacccaacgt cttcgttgtt cgttgaaccc
    35581 gtagtgatac gggcgtatga ctgaaagcac atcatacggc ttggcagagc ttaagaactg
    35641 ttctcttgcg ataagcctaa gaagcacaat agagagttag agttttgcac ctctaatcgt
    35701 tagccctgac cggaggatat ttccggtccg tgctttttta ttttgtagaa agttttttga
    35761 tagtggatct caaagaaata gttaatgatt atgagctaaa tttttgtggg aaaaggtgta
    35821 aagttgagac taattttaag catttacccg aatttatgat tttatttgat ataagagatt
    35881 tgtatcatct tctaggtatt cataagttga aaacaaagta tcgcgcaaca aattgggttg
    35941 aagctgtgaa agcagatgtt ttcctcttat cgaattattc aaaacatcca aattttagag
    36001 aagttcttcc tagagtcgat aattataatt ttttatatga aatattctat cagtttagag
    36061 ttaacgtctg tattttagat aaggatttaa ctaaaaatac aatgaaattg agtgttgttt
    36121 tttataaaga caacaagaag aaattagttg ttgtaggatt aaaaagagat gagacagggg
    36181 tctttaggcc agctacgttg catgagagcc gaaacaaccc ctacaagcga attcggcata
    36241 ctgctataaa atcaataact tggatttaga acataccttt tgtgctgatt acctctattg
    36301 acacactcgc ataatcatga gataatcaca gtagcaagaa tcgcctgaaa gt
//
LOCUS       DATRQA010000004        33536 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.004, whole genome shotgun sequence.
ACCESSION   DATRQA010000004 DATRQA010000000
VERSION     DATRQA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33536
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.004"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            34..387
                     /locus_tag="VBD27_000149"
     CDS             34..387
                     /locus_tag="VBD27_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7024194.1"
                     /translation="MNTRIPYLVALSGKTLKQISEETGIAYPTLTSYNQGVRNPKKNN
                     AVILAKYFGVSVGYLLGDEKEPNLINPGDEPLIPTKLVQELSSINYQKEEVLQEYLEL
                     TKKEQVILKKIREII"
     gene            427..963
                     /locus_tag="VBD27_000150"
     CDS             427..963
                     /locus_tag="VBD27_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7024195.1"
                     /translation="MLQNRLKQLMLKKHKTVEQVSQETGIKKGSLEMYILGKDIMTDD
                     LDTLTRYFQTNSIYLSGIWDIAADEKHLTHFRKLFKDSSYYFMPDVSDIFEDLDNCFY
                     NFWIQALQFTVLENNGRAERFFKELTFHPQDDCVFITVSDRYVIEALESYNQIIRHYY
                     PEGGDYTPILSFKGKNIE"
     gene            1140..1301
                     /locus_tag="VBD27_000151"
     CDS             1140..1301
                     /locus_tag="VBD27_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024196.1"
                     /translation="MTRQELIQEMMSVRQLTRYEADAAISLLEIQRIITFTPENQLAC
                     NLNSFQMGY"
     gene            1304..2374
                     /locus_tag="VBD27_000152"
     CDS             1304..2374
                     /locus_tag="VBD27_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEO7024197.1"
                     /translation="MAYGRKSLIQVQATEIHYQLPKVLVGTNYYKKLCAEAKFLYMLI
                     NDRLKLSLQTAKETGNFVDKHGDVYVIYPNNELMQDTGYGKDKIIKLKKELVKYNLLD
                     EERQGMKQPNRLYPKNVVSDPALLEMAFEDAPNPWEIYEVENNDFKNSEIPTSRSRQN
                     RNQEVGNLEPSKNNLIKNDNRINDTNRYQGDASLDILSASDSFQMGQHGFLSSKTINR
                     LSLFGKEAKLLENKIYQAKRQVEKDYSHLLQHQEIIYGEVWSQELEREVDKLIFKIKT
                     GENEGKAIKNIPGYFYKMMVRFWKMALLIEKEQGFIALSNQSDYAKSFPEECPSLISY
                     YYPNKLSEKELNQYLTTLRMEE"
     gene            2376..2561
                     /locus_tag="VBD27_000153"
     CDS             2376..2561
                     /locus_tag="VBD27_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024198.1"
                     /translation="MLQDEFRPIPHKVLTIGRSPKESVIAIPKAIFNLPPEEKAQALK
                     ELEEDMEQHKAKGGDST"
     gene            2558..2755
                     /locus_tag="VBD27_000154"
     CDS             2558..2755
                     /locus_tag="VBD27_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7024199.1"
                     /translation="MTTITQNRVKELRKQAGLSQQALADQIGVMRKTISHWELGNHRI
                     STDNAKKLSQFFQVSIDDLLE"
     gene            3138..3437
                     /locus_tag="VBD27_000155"
     CDS             3138..3437
                     /locus_tag="VBD27_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024200.1"
                     /translation="MTDETKKVYLRDLRNLGKQGGATARLEDGTELFLKPDYAVRTAK
                     GYVDGIPRDVEFHLTYRSIFNQIRTIKKDGHLVARKERSPSGLVLQLTGKGYKRP"
     gene            3454..5715
                     /locus_tag="VBD27_000156"
     CDS             3454..5715
                     /locus_tag="VBD27_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEO7024201.1"
                     /translation="MNQIKIITGLTVATLSAVVGNVYAEDITPTAPVNEPQVSSETAK
                     TPQVTESQVNSAKVTADQATSDVNNQQIVVDEAQKQKDQSQQNLAKATSTVTEASTIA
                     AEATPEVVKEATKAITEAKEAVTDAEANVADAQKTEQKANQEVQSQAKTVDGNVKVVA
                     DKESEVKQAEGAVTTAQEAIDSKTANTNASEAEKAVIEKQTKLETAETNLTEAQKQDA
                     KIAEEKRLAEQEVVNKQLAVTDTQTLLKKLVTEINNEKVSTSLENQAYFNQRDGAWAR
                     YYGNYTFAATGCVPSSLAMVFTELARRQITPTEIANYLWNNSNEFNKNYGGTSGKGLV
                     QATKHFGFVPTHLASQSAIVEALQAGHHVLAAVQQDKFSPWGIQYSHEIVLRGYSNGN
                     TYVYDPYNRANIGWYPVANLWNEQSRDAIDTSSVGVPFFKITTQKMAQLEAQKAQVQS
                     FLNTAKDQLVKTQDVLRTLEATPLKTPEAQAKLNQAKEALALAQTDYAKAQEAVKLAS
                     QDLAVKEETLKNAQADLLTKQTALKDAQTVLVASQVKLADLKATLATVENNVKKAQAT
                     LTDAKAIVDQKQADLQALQNAPKILAEAQAKLVTAKNDLANKMAILDEAVAKLKSLQA
                     VQAEAQKQYHVVFEAYKAVRDAKEQAKLAESYNHIIAGGGEAIPVVDETGKITGYVDG
                     SQKAVANEVTLALTSNGAPLESPVNKENQNVTKSSQALPHTGEAGLSLLSIVGAGIIS
                     TLGLVSLKKRRTH"
     gene            5735..6457
                     /locus_tag="VBD27_000157"
     CDS             5735..6457
                     /locus_tag="VBD27_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7024202.1"
                     /translation="MSKKQLLLLTCVSTIALVGTTAFAEDVVPVDPAVPSTEVVTPPN
                     PVDPSLPTDTTDPSTPVAPEKLPDTPKPAEPERPKEELPAPVDPTTPSAGKEDKPETV
                     LGQAETPKEEVKPENPKSSEDKKKEELVPDNVSKDIIEKVDTTTGEVVFKPIPVTPDK
                     TVIGTKDSQVIIRDQAGRTQVVTPESLGGHVNPNGTVTLKDATGAEKTLPKTGDKQSV
                     LGYLGMMLISVLGYAVKKKVSH"
     gene            6510..9332
                     /locus_tag="VBD27_000158"
     CDS             6510..9332
                     /locus_tag="VBD27_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7024203.1"
                     /translation="MNSQETKGHGFFRKTKAYGLVPVLALGAVALMGTTSVSAEEVTA
                     PAKPAQTVVTTLTAPTKNKAQTVTSTELNQSVVEAKEAGVNVTTTAPVSHVDVTSAQA
                     DLANQTQAVKDATAKAQANTQAIKDATAENAKIDAENKAEAERVAKANKAGQAEVDAR
                     NKAGQAAVDARNKAKQQAQDDQKAKIDAENKAESQRVSQLNAQTKAKIDAENKDAQAK
                     ADATNAQLQKDYQAKLAEIKSVEAYNAGVRQRNKDAQAKADATNAQLQKDYQAKLALY
                     NQAKKAKEEADKQTINNVAFDIKAQARGVDNKEYGNSIMTAKTKPDGSFEFNHDMIDG
                     VKTIGYGKLTGKVNHHYVANKDGSVTAFVDSVTLYKYEYRNVAQNAAVNQNIAFRVLT
                     KDGQIIFEKKHNGNSTFAETLNKTLQLNLKYELKPHASSGNVEVFKIHDDWVHDTHGS
                     ALVSYVNNNDAVPNVVIPEQPTPPSTAKVTPEAEKPVPEKPVEPKLVTPALKTYTPVK
                     FVPKEYKPEPITPEVFAPEKYNPVLPKIKTHVAVPEKVNYKVSVHPVKVPKANPTKTV
                     VDENGQSIDGKSVLPNTTLNYVAKQSFSQYKNIKASAEAIAKGFAFVDQPNEALGELT
                     VKSIKASNGDDVSSLLEMRHVLSKDTLDQKLQSLIKEAGISPVGEFYMWTAKDSQAFY
                     KAYVQKGLDITYNLSFKVKKEFTKGQIQNGVAQIDFGNGYTGNIVVNDLTTPEVHKDV
                     LDKEDGKSINNSTVKLGDEVTYKLEGWVVPTGRSYDLFEYKFVDQLQHTHDLYLKDKV
                     VAKVDITLADGTVISKGANLSQYTETVYNKETGRYELAFKKDFLAKIVRSSEFGADAF
                     LVVKRIKAGDVYNTADLYVNGYKVKSETVVTHTPEKPKPVEPQKVTPKAPALPHTGEA
                     SVAPLIAIGAIILSALGLAGFKKRQK"
     gene            9626..9853
                     /locus_tag="VBD27_000159"
     CDS             9626..9853
                     /locus_tag="VBD27_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024204.1"
                     /translation="METAVRVVTAMGAILAIVGLGWVLTGAFDYFAGRKNGSPQMMDQ
                     GFTSMISGGALAVISAGVAAAIVAAMRAISF"
     gene            9958..10539
                     /locus_tag="VBD27_000160"
     CDS             9958..10539
                     /locus_tag="VBD27_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024205.1"
                     /translation="MSESFDYVAFARDFEKRHGRPPTAEELEKANVSVATVITSDNQK
                     IDFRMVDSLDYVEGYKDKSSFGERLKTGLSFVIRNFFRVLLIVIQTPVYLTLFFFNLI
                     KSTIGVFVIWFVSKFVLGWLVGIIAGLIYGFDLYKNPFPSPIKDIVDFSFGVNFFEDA
                     VPNFFPHPVADAWIIGITIVFLALVMTFSKSET"
     gene            10590..11342
                     /locus_tag="VBD27_000161"
     CDS             10590..11342
                     /locus_tag="VBD27_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024206.1"
                     /translation="MKDILSELTKGLGSYNSSVNNSMTAINKSLMAIGYILVSILFLL
                     ELLSWYRFIRNQGGEMSWKLFLEVAVKYFIAYFLVSQSGAILNAILWFTNGVTKLIGV
                     DLSGDNMFEFVWLKKGNLGVRILVNGLAAIAGVGAMISIKVMVLLRFIELYFYKAIAP
                     VIVAFWMGDDTRHIAKNFLKTFGAIALQSAVIVLLLAIWQGLNIDAVIKISKDSWVGA
                     FAAGFSYIGKCIVFLILLFGSQRKAKQLLQVS"
     gene            11379..11675
                     /locus_tag="VBD27_000162"
     CDS             11379..11675
                     /locus_tag="VBD27_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024207.1"
                     /translation="MNLEKLKQRRDELIRDNEWMDGLIKEKEEKLWELKVRSITASEL
                     ARSAMQSAMRTAGIVDSFYGPSAQSSDNQANKAVEAVHNKSCFFMLERKSDRND"
     gene            11668..12126
                     /locus_tag="VBD27_000163"
     CDS             11668..12126
                     /locus_tag="VBD27_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024208.1"
                     /translation="MINQGQEYQYFKDKISHLEREVSRLSPYEYEHRLLKDVIADCLL
                     QGQITVSELPQAIRLIQGDDLFYTYAWRFVEATGDCQAGITILKILQDDLNYFFAIGK
                     LSQKQYSQWLEKWLSFLERGRIAFKGEKDFERYFQDQKEANRSLFSDFNL"
     gene            12188..12547
                     /locus_tag="VBD27_000164"
     CDS             12188..12547
                     /locus_tag="VBD27_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEO7024209.1"
                     /translation="MNKLGSEFFKAIDQFERGIIGSFTWRQILMFFGIVLGVGLATVI
                     SLLQLPSILFYLSLALTVVPAFIYGIKKDEHLKEVITFRLKVQERAYQTEYESEENYG
                     AFIPQKGVQEWNDPNES"
     gene            12528..14888
                     /locus_tag="VBD27_000165"
     CDS             12528..14888
                     /locus_tag="VBD27_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEO7024210.1"
                     /translation="MTLTKADKERAKQLQKALKASTQNTIQYTSLFEDGLMHIVDEAY
                     SKTWQLGDTNYITGSEDEKLDIIEYYVDALNGLDSENTYQLTILNRPVPTTLLDQITY
                     DLEGDERDVFRKEYNNIIRSRFAKDQNNFKVEKFVTVSTRAKDRKQAYRKLNDVDTHF
                     TTQFQLVDVLLKSLDGTERLNIFADLLRGNPYLDVDYKDVRISGLSTKSFIAPGRLWF
                     QPDQFRMDKKYCKILFARTFPAFLNDRLIKSLTDIGIELSISIHAKPYDVAEAVKKVN
                     TAEAGVKMDMVKSQVAAAQKGISGNLAVSSVAQDTADEAEKWKTEINDNDQKMFSGVF
                     LVMVKADSAEELADYTSRIKQAGRKHMIEFEEIYYHQEEALNTMLPIGKTYIDVKRRF
                     MRDMTTTNIATQIPFTNTDLQSQSPLANYYGQNQISNNIISLDRQRDLYTSSGVILGS
                     SGSGKSVFVKINEVIPAILRFPNDRVIIVDPEEEYVDIGRAFGAQVIDIYPGTKTHFN
                     LMDIPDLDKLRDEDKDFVGQKASLIMGLFENILQEVTDDDVSLIDRVTRLCYEQITDR
                     TPTLKDWHDILLEQPEEEAQSLALKSESYTKGSQDIFAYETNVDLNNQVVIFNLKKLS
                     GKLKPFAMMVIQDYIWQHVVDHKDEFVTRVYFDEMQNQFETDDQAAFFSKMYARIRKY
                     GSIPTGITQNVETLLSRKEGRNLLYNSEFLVLLKQKKTTIPYLLQTINLTDALVRYIE
                     KPKAIGTGLIIAGTTTVPFENPIPEDTELFRLVATDAYRKMEEENV"
     gene            14881..15474
                     /locus_tag="VBD27_000166"
     CDS             14881..15474
                     /locus_tag="VBD27_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024211.1"
                     /translation="MFEDVTLQLPKNEEKKLMAHYYQLHQEAIEEALAGKELWKPYIR
                     MSGLSQWLDVSTTTIVKWQREGMPHIVIDGVTLYDKRKIATWLNHFERNKIISQEKQM
                     KIFPGYLSIKHVFGTIITYSKKVVLDLIQDLRDEDIQDFSYWSPLYFLETDELGQPVY
                     LTYLVNEVGIPLYPDCYYYITLTKEQESKLESLELSI"
     gene            15498..15823
                     /locus_tag="VBD27_000167"
                     /pseudo
     CDS             15498..15823
                     /locus_tag="VBD27_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15858..18548
                     /locus_tag="VBD27_000168"
     CDS             15858..18548
                     /locus_tag="VBD27_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7024212.1"
                     /translation="MTEEKQDLRSRVVRKRQIKASVHRHNQSSARKAQKAAKGEFQIA
                     TKELKEAREAYQKAQTDFRAGIIDDEALQKAKNRLKQAQNKYKDAKKVKKKVRKTNLT
                     LSQKARQTGRRITKRTGQEILEMGLEQDDTLSDLVRTKHQIRQTRVMAKQAGRATGKS
                     VKLTYRGAKGTVKLGAKGTKYAYRQTRKGSVKATQASLRAASKTAQTLVATSKALVSA
                     ITPIIANPITWVVAGVISLLLLIVILVSSVFSSNVVQQNEFTLNQSWLHISQMDRKKS
                     SDKVDYYTDIDSILLYMNYRYGGEWEPDAKWDDGFGGKVTGALGFNHFSDALDDIWKE
                     ENSDITNLKTMAELYTNGKKWIKFSKDDLEEYKEILDSQVETGKYLAYQELSNPFYSS
                     NDDKADSEYLTVTKRYGYTDKDTIDPTTTLQAKASQKLYAVMDGKVTVTKKDLKGEKT
                     KTTNLIIEGKEARFIYYDISNSRVRTGDRVETGQELATVSGSTQKIAYAKRYGEIDDK
                     DATWLKKIRDREVSYGYTIQTKMNKKEVWVLTNPGFYFPFVKYAETTIVTQTPSEMSG
                     RAKQFYDLVKKYLPQATDNGIAAIVGCFGVESGINPKRAEGDYLSPPVGASDSSWDDE
                     SWLTLGGPAIYGGGYPNILHRGLGLGQWTDTSDGANRNTLLREFAKKEGQKWYSLELQ
                     VKFMLEGDNPYYINTFKRVATNNGDVDSLTAEFLAKWEGVPGNKLAQRQAIAKQALMW
                     FKQPTHSGGASTLQSSWNFPKEYASKLKSYPSAATVSRSLNGNTYPIGQCTWYVYNRL
                     SEAGAPAINWLGNGQDWVRNLVAKGWTFSNKPVAGAVCSTAGGFDYTMPTYGHVSYVE
                     HVNEDGTFLVSECNVMGIQNQIHWAVKLNQSYYTFAIPPK"
     gene            18569..19174
                     /locus_tag="VBD27_000169"
     CDS             18569..19174
                     /locus_tag="VBD27_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024213.1"
                     /translation="MIDTFKVKWFKRGLSLLALLLVFFLGLGFGQMGNSSHSKDRPKQ
                     TATKKTRTNTITQTQVKEFLVAYYTKKDLEENRNRYKDYMTEGLYNATISEEDKAQNK
                     AYKGFVVNFEFQDANIYIDQVHKQVICEVTYTNDLLQKKNNKDGAQIGVVNHTTIQLV
                     YTKLNGKNLINQMSTILLTDSANPSEETESYGAIAPSNTKD"
     gene            19194..19466
                     /locus_tag="VBD27_000170"
     CDS             19194..19466
                     /locus_tag="VBD27_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024214.1"
                     /translation="MINEEHMNQTSSSKSRRQKSTQERLEDKIKQLEKAQESQDALQK
                     KIKTLKDDIAILEGKRQQEIMAEYGIDLQGLEAILATHKEKLGGEG"
     gene            19466..20251
                     /locus_tag="VBD27_000171"
     CDS             19466..20251
                     /locus_tag="VBD27_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024215.1"
                     /translation="MFRIQNQSDGKEQEFQNLAALLYTLEGEEKRCLQLNMRATFYLF
                     HLDKNEEVLESMELTIPSQEDKEVQELIGDFGLKGETKKFFWQRSMDKKPIPIKTSPN
                     NHQELPSKTGALTLLKRLVWLLPMVLSVVSIYLSLETLQLVKTQSQVTPKVETVVTNQ
                     KADVFCRYFISSYFSQSPNLGDYLSEKLSIDDLKTDKVTPVSVLLESQEIKGDTTLVT
                     YVVNIKEAQDHVSSKRLTLAVRADKKAQYGYLVIKKLRLTAYP"
     gene            20273..20764
                     /locus_tag="VBD27_000172"
     CDS             20273..20764
                     /locus_tag="VBD27_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3801 domain-containing protein"
                     /protein_id="HEO7024216.1"
                     /translation="MDQERVMEKGSRVTLETGKHLLQFLAYAVSQGAKIYTEHKQSGE
                     MSWKDFNQLPRTKDHIDFEEAEVNLEKLRKELKKSGVNFHFKKNGDGTKQVWFEAINQ
                     QVIQEALRKINQEIVSNPKEALDKYMKKPDELTPKQQIEKLKKTSKKSTEAVKTKKKG
                     KGI"
     gene            20764..22809
                     /locus_tag="VBD27_000173"
     CDS             20764..22809
                     /locus_tag="VBD27_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEO7024217.1"
                     /translation="MELKRKRLLPYLLLSLLLAYLTHRAYVLYCMAPQADMTNLFAPY
                     TYVFEKITEPPYFYWNTSPLAILSALVGFFVGLLFYLKIRLDGNYRHGEESGSACFAT
                     AQELKGFQDEESRNNMIFSREAQMGLFNKRLPFERQLNKNVLVAGLPGDGKTYTYVKP
                     NLMQMNSSFIVTDPKGLLVREVGTMLKENGYQIKIFDLVNLTNSDMFNPFRYMRSELD
                     IDRITEAIVEGTKKGDREGEDFWNQAKLLLNRALLGYLYFDSQVRHYEPNLSMVADLL
                     RNLKRPNEKELSAVEKMFKQLEEKLPGNYACRQWELFNSNFEAETRTGVLSIVATQYS
                     IFDHDAVTNLIKTDTMEMDTWNTEKTAVFVAISETNKAYSFLASTFFTVVFDQLTHSA
                     DAIIQGEKKGYELKDLLHVQFYFDEFANCGKIPHFNEVLASIRSREMSIKIIIQAISQ
                     LDSLYGVPARKSMVNNCATLLFLGTNDEDTMKYFSMRAGKQTIIQTSYSEQRGHRISG
                     TTSRQAHQRDLMTPDEIARIGVDEALVFISKQNVFRDKKAMVSNHPMKDRLANHYQDK
                     TWYHYKRFMEEGAEFIDAVMTGQIPEDRIWAPDMSDYQAFLEENGFDNTRQSAPETSV
                     HNQVPVTNPEMPSESPSQTPETSTKLVDMDTGEIFELPPEDREGFGEVSFDDQRVEI"
     gene            22851..23216
                     /locus_tag="VBD27_000174"
     CDS             22851..23216
                     /locus_tag="VBD27_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024218.1"
                     /translation="MTPQILRRLDVKKQFIEAIDPFVHRQTLKPKAVNSSKTTMSIQR
                     YNHAGTKIQLRIGYSKVLIRIFSNGKINLTHYDLFFDREETLEITDAFDNGVYTQDEV
                     DGFIKQAKTFIKQALKGEV"
     gene            23218..26457
                     /locus_tag="VBD27_000175"
     CDS             23218..26457
                     /locus_tag="VBD27_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEO7024219.1"
                     /translation="MANTYDLISQRIEAQRQKLIAIDIVAVASSLGLNLKQGSGGHLY
                     WDEHDSFHIYPQTNTFRWWSRSMGTNTIDLVQVIQEELTGKKPNFRETVNFLETGQFE
                     SVTVTPVVREPFKHYLAPYEHHNFDLGRQYLKEERGLSDETIDFALASGSMSSATLKK
                     GDYFEPVIIFKSFAEDGRMIGGSLQGIVENKVQHPERGRLKQIMKHSDGLAGFHLDVG
                     TPKRLVFSEAPIDLLSYYELHKESLQNVRLVAMDGVKKGVISRYTADLLTDGQYSQTM
                     PRESIRGAIDAINQTTRILKNNPNLITIAVDNDEAGRNFIKELQEDGIPITIDLPPRK
                     EHQSKIDWNNYLKQKKGLLKMPQTEGTQKALDITQEPNTGGERFNRNSSFLGEESSPR
                     TAPKPVVDEPQPDFPTNVPFHFSIDRSKKSTTKLRSSYEYASDKQLRQLNLFADDIRK
                     SANWYLDTVADSKVTYFYQDGDEITTLQVEFEKKNWMHLTGLAPVYSNWVPSLSESFI
                     EDIANGKGQFANLTLGRGVMEKIQILPILPEVLETDSFVFDDLSSVEKFQRLDANKAI
                     ITDNEQLILAFRFDDGNFYPATLLKPTKALNIQLNETNQEKIVLGVFIEKDGLVKTHK
                     VNEDYIKDGGNELKSIIENDELEPIQTAPLERNVQQEKIFPKDSDGDGLTDDEEIALG
                     TNPFSSDSDGDGTPDNVEKANGTDPTNASDNEVTRQQEVNKRDFTLSEMIKAKNTAAL
                     NQHLQDGIKQYFDSNTYKQYLEGMAHFNNYSPRNIQLIMSQFPEASMVASFQEWKKRN
                     GSVNKGEKAIYIQAPVAVIQKDKNGKPILNPETGEKETITYFKPVPVFDIKQVSPQEG
                     KELNLPKAMGTIPEQLDKDYYQNVYRSLRDISQNNNKVPIRFRELGQEDGFYSPQTNE
                     IVIKKGMSYERTLSTLIHEMAHSELHNKQSLTERFDGQLTRSTKELQAESIAYVVSNH
                     LGFDTSQESFPYLVSWSKEKDGLANLTAQLEIVQEEAKSLMERIDQQLSQYQTVTLNK
                     ETQQLTKQEMKKQAHPFYQSLAAAKTSRTQVTTQEKEASVKKDNRPTMP"
     gene            26509..26808
                     /locus_tag="VBD27_000176"
     CDS             26509..26808
                     /locus_tag="VBD27_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024220.1"
                     /translation="MLTEEMMTTTEMILMTYLFEIDEWLKDKKVIQQQQAQLTKEELV
                     TVIKNDLTTFQANQATDYEKKLQTTLTTLEALSEAQFQKMKLEILSWGPNVKHVD"
     gene            27151..27711
                     /locus_tag="VBD27_000177"
     CDS             27151..27711
                     /locus_tag="VBD27_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024221.1"
                     /translation="MITPSELTHRIEHTTLSEAIELFEDKVLRKSLNNYDDWYKRDVQ
                     KEYERINYDGAFFFFVEPDLGSSRGGVSDVIIEEQEKVALLLLLVEAYERYIDVNTGI
                     KDWLGYDCIFCDVVVSNETAAKRLTQMEYEAIKDLIVTVIDHYVPSMTVMETDEYKEF
                     KQGQTPNDTVIDNVQITLPLFNKREK"
     gene            27714..28088
                     /gene="mobC"
                     /locus_tag="VBD27_000178"
     CDS             27714..28088
                     /gene="mobC"
                     /locus_tag="VBD27_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEO7024222.1"
                     /translation="MVEKRRTRINRFELRTTDGEANKLRRRITLSGKKTFQAYALKML
                     LEGKIETYDYSELRQLRIEVNRIGQNINQLVRYVNTFEEFDQELFTALQEEVKELQQM
                     IVQEFKTKEVAKQHGSDQSLSD"
     gene            28060..29700
                     /locus_tag="VBD27_000179"
     CDS             28060..29700
                     /locus_tag="VBD27_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEO7024223.1"
                     /translation="MVVTKVFQIKQIKNLKRAIDYITRDDATLIKVEQHDEDDAFDYT
                     LTADGEVHKKLISGHHLIDPGDRDAIYDDFILLKQSVDELYDNATLSDLKNDNRVLAH
                     HIVQSFSPDDKLTPEEVNEIGRKTALEFTGGDYQFVVATHVDKGFLHNHIIFNTTNEV
                     TLKKFRWQKNTARNLFQISNKHAELYGAKILEPKLRTSYTDYSAWRRQNNFSYEIKQR
                     LNFLLKHSISIEDFQQKAKALDLHIDFSGKFAKYRLLVPLDGKLQEKNTRDRTLSKKR
                     IYSFEQIQKRVAMNEVVYTVDHIKEKYEEEEAKKAEDFEMKVRIEPWQISEITRQSIH
                     LPVTFGLEQKGTVSIPARLLDQNEDGSFTAYFKKKDYFYFINPDKSTDNRFIQGSTLV
                     KQLSLNSGEVILTKNWEMTELERLVKEFNFLSANKVTNSKQFQEMQERFLEQLEKTDQ
                     TLAQLDSKVAYLNKVLGALSDYQDGLVPSEVTMAILEKAKVAKNTDRNLIRKEIKELQ
                     IEREALQEARDRIVKDYDFSHKLTQEYSPKESRKKSII"
     gene            29837..30244
                     /locus_tag="VBD27_000180"
     CDS             29837..30244
                     /locus_tag="VBD27_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024224.1"
                     /translation="MGKSYYYVEVETIQGESLIFQLPNDLQGAMRDYRHENPETWEAL
                     LKEALINIPSASYTKANHYQPIIRLAKVKKSFSLKKQQRRRSRGQFLTKDNWQQKGFK
                     HFRDSLRFIQHDYPFKIRVRLLLDFWRWKRRSY"
     gene            30275..31558
                     /locus_tag="VBD27_000181"
     CDS             30275..31558
                     /locus_tag="VBD27_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HEO7024225.1"
                     /translation="MTRFDEKIFVIEMAKLREKEFLKRIRDYDEQIATTMRQHGYKRV
                     DSSERTVLFTFGEITFARNRWRKGRKTRYPVDEWLGLKPYMRYSPELMLHMAEHASKL
                     SYREVCRTIETAYGLLVTKDAVLKAVKLAERLLIEKEHYRFLQHVEHPQKIQAERIYL
                     EGDGVMVKTTSGGDERHNTDLAHFLIHTGSRKSGKNRYSLTNKHEIINANYDKAREEL
                     LDYLYNHFEITDKTILITNSDNGKGYTKRVFQEVKKALGIKHHEHFWDSYHLNKQLKD
                     FFKPYPYVLQDLAFKAIQTHKKSLLETVFDTVETLIETEEDYINYYAFRQKLFRNFRD
                     TRPSQLRGLTSRGIGAMESQHRKVTYRMKHRGMYWSVTGACTMAKIILLERINKLGDL
                     FFGDWRKQYKRYQGRGLGAGHLVNHYPHDAVLIRR"
     gene            32026..32451
                     /gene="rplK"
                     /locus_tag="VBD27_000182"
     CDS             32026..32451
                     /gene="rplK"
                     /locus_tag="VBD27_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO7024226.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGEPNKTKVATITR
                     AQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVTD"
     gene            32555..33244
                     /gene="rplA"
                     /locus_tag="VBD27_000183"
     CDS             32555..33244
                     /gene="rplA"
                     /locus_tag="VBD27_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO7024227.1"
                     /translation="MAKKSKNLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     SYNLNIDVKKADQQIRGAMVLPAGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KIQGGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDDAKLVDNFKAFNDVIVKAKPATAKGTYITNLSITTTQ
                     GVGIKVDPNSL"
BASE COUNT        11711 a         6030 c         6769 g         9026 t
ORIGIN      
        1 tgctgaagta atgcattgag aaaaggagat gaaatgaata caagaattcc atatctagtc
       61 gctttaagcg gtaaaacact gaaacaaatt agcgaagaaa ccggaatagc ttatccaaca
      121 ttaacaagct ataaccaagg tgtacgcaat cctaaaaaaa ataacgcagt tattcttgca
      181 aaatattttg gagtatcagt tggatactta ttaggagatg aaaaagagcc taacttgata
      241 aatccaggtg atgaaccttt aattccgaca aaattggttc aagagctatc ttctatcaat
      301 taccaaaagg aagaagttct acaagaatat ttagaactga ctaaaaaaga acaagttatt
      361 ctcaaaaaaa tcagggagat tatttaatga cagataacga tattcaaatc aatgaaggag
      421 atcaaggtgc tacaaaatcg tctaaaacaa ttaatgctta aaaaacacaa aactgtagaa
      481 caagttagtc aggaaacagg gattaagaaa ggttccctag aaatgtacat tctaggaaaa
      541 gacattatga ctgatgatct tgataccctt actcgctatt ttcaaacgaa tagtatctat
      601 ctcagtggca tttgggatat tgcagctgat gaaaaacatc tgactcattt caggaagcta
      661 ttcaaagata gtagttatta tttcatgccg gatgttagtg atatttttga agatttagat
      721 aactgtttct ataatttttg gatacaagct ctgcaattca cagtcttaga aaataatggc
      781 agagcggaac gtttctttaa ggaattaacc ttccacccac aggatgactg tgtttttatt
      841 accgtctcag acagatatgt tatcgaagca ctagaatctt acaaccaaat tattcgccat
      901 tattatcctg aaggtggaga ttatacaccg atactctcat ttaaggggaa aaacatagag
      961 taacaccaat ttataaggta agagatagcc ttggaatttt atccggagtt aatgagtgat
     1021 gtattaggag ggtaagatga ttcaggaaga aagagacaaa gggtttgaca tctaaaaagg
     1081 gaacatattg aacaatagta tttaaatact aaagtacata agagaaagga acaaacttaa
     1141 tgacaagaca agaactcatt caagaaatga tgtctgttag acaattgacc agatatgaag
     1201 ctgatgctgc tatatcactg ctagaaatac aacggataat cacttttaca ccagaaaatc
     1261 agctagcttg taatctcaac tcatttcaaa tggggtatta aatatggcgt atggaagaaa
     1321 aagtttaatc caagtccaag cgacagaaat ccattatcaa ttacctaaag ttttagttgg
     1381 aaccaactat tataaaaaat tatgtgcgga agcaaaattt ctctacatgc taattaatga
     1441 tcgtttgaaa ttatctcttc aaacagctaa agaaacagga aattttgtag ataaacacgg
     1501 tgatgtctat gttatttatc ctaataatga actgatgcaa gatactggtt atggtaagga
     1561 taaaattatc aaattaaaga aagagttagt gaaatacaac ttacttgatg aagaacgaca
     1621 aggaatgaag caacctaata gactctatcc taagaatgtt gtatctgatc cagccctatt
     1681 agaaatggct tttgaagacg ccccaaaccc ttgggaaatc tatgaagtcg aaaataacga
     1741 cttcaagaac tctgaaatac cgacttcaag aagtcgtcaa aaccgaaatc aagaagtcgg
     1801 aaatttagag ccaagtaaga ataatctaat taagaatgat aatagaatta acgatactaa
     1861 tcgataccag ggcgatgcct ctctagatat tctttcagct tctgattctt ttcaaatggg
     1921 ccaacatgga tttctatcct caaaaacaat aaaccgtctc agcttatttg gtaaagaggc
     1981 taaactcctt gaaaataaaa tctaccaagc taagcgtcag gttgaaaaag actacagtca
     2041 tcttttacag catcaggaaa taatttatgg tgaagtctgg tcgcaggaat tggaacgtga
     2101 ggtggacaaa cttatcttca aaatcaaaac tggcgagaat gaaggtaagg caatcaaaaa
     2161 tatcccaggc tatttctaca agatgatggt gcgcttttgg aagatggctt tgttaattga
     2221 gaaagaacaa ggtttcatag ccttatcaaa tcaaagtgac tatgccaagt cattccctga
     2281 agaatgtccg agtctaatca gctattatta tccaaataaa ttgtcagaaa aggaattgaa
     2341 tcagtattta actacactta ggatggagga ataacatgtt acaagatgaa tttagaccta
     2401 ttccccataa agtcttaacg attggtcgta gccctaaaga aagtgtgatt gccattccta
     2461 aagctatatt caatctccca cctgaggaaa aagctcaagc tcttaaagag cttgaagaag
     2521 acatggaaca gcacaaagcg aaagggggtg actccacttg acaactatca ctcaaaatag
     2581 agtaaaggaa ctacgtaaac aggctggcct ttcccaacaa gcattagcgg atcaaattgg
     2641 cgttatgcgt aaaactatct cgcattggga attagggaac cataggatat caacggataa
     2701 tgctaaaaag ctctcacagt tctttcaggt atcgatagat gacctactag aataaaatgt
     2761 tctgatgtgg gaaaagacaa agattaatct gaaaagttaa ttttgtcgtc acgcctctag
     2821 tacgaaccta aagtaaaaat taacaaccta aaaaggaatt gaccgaaatg tcgttaaact
     2881 aagtagatca ttgacaatta aacacaaggc ttgtcctgat acagcgtggc tatggataaa
     2941 agaaaaatgg gggcgagtag caagctagaa aaggatatct agtccagcca cagtaacgaa
     3001 aaagcatgat tttatgaaat cataagctaa aggagtaggg ggtcctagac tataacccca
     3061 gcacatgaaa caccgagata ggtttacgaa actatccagc acagcgtaaa tcgcttttca
     3121 acttcaagga gaaatctatg acagatgaaa caaaaaaagt ctacctaagg gacttacgaa
     3181 acttaggaaa gcaaggtggt gctacagcac gcttagaaga tggtacagaa cttttcctaa
     3241 agccagacta tgctgtaaga actgctaaag gctatgtgga tggtatacct agagatgtag
     3301 agttccattt gacttataga agcatcttta atcaaattcg taccatcaag aaagacggtc
     3361 acctagtagc tagaaaagag cgaagtccat ctggacttgt acttcaattg acaggaaaag
     3421 ggtataagag accataaaag gagattagac gaaatgaatc agataaaaat tatcacagga
     3481 cttacagtcg caacactatc cgcagtggta gggaatgtat acgcagaaga tatcacaccg
     3541 acagcaccag ttaatgaacc acaagtatca agcgaaaccg caaaaacacc tcaagttaca
     3601 gaaagtcaag ttaacagcgc aaaagttact gctgatcaag caacaagtga tgttaataac
     3661 caacaaattg tagttgatga ggctcaaaag cagaaagatc aatcacaaca aaatcttgca
     3721 aaagccacat caacagtaac tgaagcttct acgattgctg ctgaggcaac acctgaagtt
     3781 gttaaagaag ctactaaagc tatcactgaa gcaaaggaag ctgttacgga tgctgaagct
     3841 aatgtagccg atgcacaaaa gacggaacaa aaagctaatc aggaggtaca atcacaagct
     3901 aagactgttg atggaaatgt taaagttgtt gctgataaag aatccgaagt gaaacaagca
     3961 gagggtgctg taactacagc tcaagaagct attgatagca aaaccgctaa cactaatgca
     4021 tcagaagcag aaaaagcagt gattgagaag caaacaaagc ttgaaactgc tgaaacaaat
     4081 cttacagagg cacaaaaaca agatgccaaa atcgctgaag aaaaacgact tgcagaacaa
     4141 gaagttgtta ataaacaatt agctgtgaca gatacacaaa ctcttttgaa gaagcttgtt
     4201 actgagatta ataacgagaa agtaagcact agtttagaaa accaagccta ttttaaccaa
     4261 cgtgacggtg catgggcacg ctattatggc aattatactt ttgccgctac tggctgtgta
     4321 ccaagtagtt tagcaatggt atttacggag ttagctagac gccaaattac tccaactgaa
     4381 atcgccaatt atctttggaa caattcaaat gagtttaata aaaactatgg tggaacaagt
     4441 gggaaagggt tagtacaagc tactaaacat ttcggttttg taccaactca tttagcatct
     4501 caatctgcta ttgtggaagc cttacaggca ggacaccatg tcctagcagc tgtccaacaa
     4561 gataaattct ctccttgggg tatccagtac agccatgaga ttgtcttacg tggttactca
     4621 aatggcaata cttatgttta cgatccttat aatcgagcta acattgggtg gtaccctgtt
     4681 gctaatttgt ggaatgaaca aagtagagac gcaattgaca catctagtgt aggcgtacca
     4741 tttttcaaaa ttacaacaca aaaaatggcc caattagaag ctcagaaagc acaagttcaa
     4801 tcttttctaa atacagctaa agatcaatta gttaaaacac aagacgtatt aagaacacta
     4861 gaagcaacac ctcttaaaac accagaagca caagctaagc ttaatcaagc caaagaagct
     4921 ctagctcttg cacaaacaga ctatgctaaa gctcaagaag ctgttaagtt agctagtcaa
     4981 gacttagctg ttaaagaaga aacacttaaa aatgctcagg ctgatttatt aacgaaacaa
     5041 acagccttga aagatgctca aactgttctc gtggctagtc aagttaaatt agctgacctt
     5101 aaagcaactt tagctactgt tgaaaataac gttaagaaag cacaagctac tttaacagat
     5161 gccaaagcaa ttgttgatca aaagcaagct gacttacaag cattgcaaaa tgcccctaaa
     5221 attctagcag aagcacaagc taaacttgta actgctaaaa atgatttagc taataaaatg
     5281 gctattttag atgaagcagt cgcaaaacta aaatccttac aagccgttca agctgaagcc
     5341 caaaaacaat accatgttgt ttttgaagcc tataaagcag tacgagatgc caaagaacaa
     5401 gctaaacttg cagaaagcta taaccatatt atcgcaggag gcggtgaggc tatcccagtt
     5461 gttgacgaaa caggtaaaat aactggatat gtagatggta gccagaaagc agttgctaat
     5521 gaagtgactc ttgccttaac tagtaacggt gcaccccttg aaagtccagt taataaagaa
     5581 aaccaaaacg taacaaaatc atctcaagca ttaccacata ctggcgaagc aggcttatcc
     5641 ctcttatcaa ttgtaggggc agggattatc tcaactctag gccttgttag cttgaaaaaa
     5701 cgtcgcacac attaattaat gaaaggaatt tactatgtca aaaaaacaat tattactctt
     5761 aacttgtgtt tccaccattg cacttgtagg aactaccgca tttgcggaag acgtcgtccc
     5821 agtagaccca gcagtaccat ctactgaagt ggtcacacca ccaaacccag tagatcctag
     5881 tttaccaact gatacaacag atcctagcac cccagtagca cctgagaaac taccagacac
     5941 cccaaaacca gcagagccag aaagaccaaa agaagagcta ccagcgccag tggatccgac
     6001 aactccatca gctggtaaag aagacaaacc agaaactgtt ctaggtcagg ctgaaacgcc
     6061 taaagaagaa gtaaaacctg aaaaccctaa gtctagtgaa gacaagaaaa aagaagaact
     6121 tgtcccagat aatgtttcaa aagacattat tgaaaaggta gataccacga caggtgaagt
     6181 ggtctttaaa ccgattccag ttacaccaga caaaacagtt atcggtacta aggacagtca
     6241 ggtgattatt cgtgaccaag caggccgtac acaagtcgtt acccctgaga gccttggtgg
     6301 ccacgttaat cctaatggaa ccgttacact taaagatgct acaggtgctg aaaagacgct
     6361 cccaaagaca ggtgataaac aaagcgtgct tggttacttg gggatgatgt taatttcggt
     6421 tttaggctac gctgttaaga aaaaagtatc gcactaatca catgacctag aatgtcacta
     6481 aacttaactt agaaaatgga gaaaacaata tgaattcaca agaaacaaaa ggtcacggat
     6541 tttttagaaa aacaaaggcc tatggtttag ttcctgtcct tgccttgggg gcagttgccc
     6601 ttatgggcac aacaagtgta tcagcagaag aagtaaccgc accagcaaaa ccagcccaaa
     6661 cagttgtaac aactttaact gcaccaacta aaaataaggc acaaactgtt acttcgactg
     6721 aacttaatca gtctgttgtt gaagcaaaag aagcaggcgt taacgttaca acaacagcac
     6781 ctgtatcaca tgtagatgtt acaagtgcac aagctgattt agctaatcaa acacaagctg
     6841 ttaaagatgc tactgctaaa gcacaagcta atacacaagc tattaaagat gctactgctg
     6901 aaaatgcaaa aattgatgct gaaaacaaag cagaagcaga gcgtgttgca aaagctaata
     6961 aagcaggtca agcagaagta gatgctcgta ataaagcagg tcaagcagcc gttgatgcac
     7021 gtaataaagc aaaacagcaa gcgcaagacg atcaaaaagc aaaaattgat gctgaaaata
     7081 aagcagagtc tcaacgtgta agtcagttaa atgcacaaac taaagcaaaa attgatgctg
     7141 aaaacaaaga tgcacaagca aaagcagatg cgactaatgc acaattacaa aaagattatc
     7201 aagctaaatt agcggaaatc aaatcagttg aagcttataa tgcaggtgta cgtcaacgta
     7261 ataaagatgc acaagctaaa gcagatgcga ctaacgcaca gttacaaaaa gactatcaag
     7321 ctaagttagc actttataat caagctaaaa aagcgaaaga agaagcagat aaacagacta
     7381 ttaataatgt tgcttttgat atcaaagctc aagccagagg cgttgataat aaagagtatg
     7441 gaaactcaat catgactgca aaaactaaac ctgacggaag tttcgagttt aaccatgata
     7501 tgatcgatgg tgtgaagaca atcggctatg ggaaattgac aggtaaagtt aatcatcatt
     7561 atgttgctaa caaggatggc tctgtgacag catttgttga ctctgtcact ctttacaagt
     7621 acgagtatcg taatgttgct caaaatgctg ctgttaatca aaatattgct ttcagagtct
     7681 taactaaaga cggacaaatc atttttgaga agaaacataa tgggaatagc acatttgcag
     7741 aaactttaaa caaaacttta caactcaatc ttaaatatga gcttaaacca catgcttcta
     7801 gtggaaatgt tgaagtcttt aagattcatg atgactgggt acatgatacg catgggtctg
     7861 ctttagtgtc ttatgtcaat aataacgatg ctgttccgaa cgtagtcatc ccagaacagc
     7921 caacaccacc gtcaacagct aaagtgactc ctgaagcaga aaagccagta cctgaaaaac
     7981 cagttgaacc taaattggta acgcctgctt taaaaactta tactccagtc aaatttgttc
     8041 caaaagaata caaaccagaa ccaatcacac cagaagtatt tgcaccagag aagtataatc
     8101 cagtcttacc aaaaattaaa actcacgttg cagtgcctga aaaagttaat tataaagtat
     8161 cagtgcatcc tgtcaaagta ccaaaagcca atccaacaaa aacagttgtt gatgaaaacg
     8221 ggcaatccat tgatggtaaa tctgtattac caaatacaac gttaaactat gttgctaaac
     8281 aaagttttag tcaatacaaa aacattaaag cttctgcaga agctattgcc aagggttttg
     8341 cttttgtgga tcaaccaaat gaagcgttag gagagttgac tgttaagtct atcaaagcat
     8401 ctaatggtga tgatgtatca agcttgttag aaatgcgtca tgttttatca aaagatactt
     8461 tagaccaaaa acttcaatct cttattaaag aagcaggaat cagccctgtc ggtgagtttt
     8521 acatgtggac tgcaaaagat tcacaagctt tttataaagc ttatgtacaa aaaggtctag
     8581 atattactta taacttatca tttaaagtta aaaaagagtt tactaaaggt caaattcaaa
     8641 atggtgttgc acagattgat ttcggaaatg gttacacagg taatattgta gtcaacgatt
     8701 tgacaactcc agaagtccat aaagacgtgt tagacaaaga agatggcaaa tctattaaca
     8761 atagtactgt caaattaggt gatgaagtta cttacaaatt agagggttgg gtagttccaa
     8821 caggtcgttc ttatgatcta tttgaatata agtttgtaga tcaattacaa catactcatg
     8881 atctttacct aaaagataaa gtagtcgcaa aagttgatat tactttggca gacggtacgg
     8941 ttatttccaa aggagcaaac ctatctcagt acacagaaac agtctacaat aaagagacag
     9001 gtcgttatga actagcattc aaaaaagatt tcttagctaa aatcgttcgt tcaagcgaat
     9061 ttggagcaga tgccttttta gttgtaaaac gtattaaagc aggtgatgtt tacaatactg
     9121 ctgatttata tgttaatgga tataaagtta agtctgaaac agttgtgact catactcctg
     9181 agaaaccaaa accagttgaa ccacaaaaag taactccaaa agctccagcg ttacctcata
     9241 caggtgaagc aagcgttgcc cctcttatag cgattggagc aattatctta tcagctctag
     9301 gtctcgcagg ctttaaaaag cgtcaaaaat aaccaagtta atggataagc catcgaagta
     9361 gattactttg ggtctatcca ttctctctaa caagtaacta ttggggggat ctcctttccc
     9421 ttcaaaaaac tagccaaaaa agtttactat tggttacttg ttagggtgag tggatagatt
     9481 ttggtttatc actagaatag tgaccgaaaa gtcataaaac taagtaatca ggaaaactaa
     9541 aaaaagataa gacataaggt ttcctcgttt gatcaagact agaaccctct agtcttgatt
     9601 ttttgaagaa agaaggtatc aaaatatgga aacagctgtc cgtgttgtaa ccgctatggg
     9661 tgcaatactt gcgattgtcg gactcggatg ggtcctgaca ggcgcttttg attattttgc
     9721 agggcgtaaa aacggaagtc ctcagatgat ggatcagggg tttacttcta tgatttcagg
     9781 aggtgccttg gcggttattt ctgctggtgt tgcggctgcg attgtagctg ctatgcgagc
     9841 tatttcattt taaaactagt ttagaaaatg tggctaaaga aacgaaaatg ggtcatctat
     9901 tgacctaaaa tatggtataa taaagatgat aaagcaaaac gagaggaagg gaagaagatg
     9961 tctgaaagct ttgattatgt ggcatttgcc agagactttg aaaagcgtca cggtcgtcca
    10021 ccaacagctg aagagttaga aaaggctaat gtttcagtgg cgactgtcat aacttcagat
    10081 aatcaaaaga ttgattttcg gatggttgat agtttggatt atgttgaagg ctataaagat
    10141 aagtctagct ttggtgaacg cttaaaaaca gggctcagct ttgtgatacg aaatttcttt
    10201 agagttctcc tcattgttat tcaaacacca gtctatctca ccctcttctt ctttaatctg
    10261 attaaatcaa cgattggtgt ttttgttatt tggtttgtat caaaatttgt attaggatgg
    10321 ttggtcggta ttattgctgg attaatttat ggttttgact tatataaaaa tccatttcct
    10381 agtccaatca aagatatagt tgatttcagt tttggtgtta acttctttga ggatgctgtt
    10441 cccaatttct tcccacatcc tgtcgctgat gcctggatta ttggcattac catcgttttc
    10501 ttagctttag tcatgacttt ttcaaaatca gaaacttaat acttaagaac ctttcgaggt
    10561 tctttttatt tttgacagaa aggagaatga tgaaagatat actttcagaa ctaaccaagg
    10621 gactaggaag ctataattcc tcagtaaata attccatgac tgccatcaat aaatccctca
    10681 tggccattgg atatatttta gtatccattt tatttttact tgagctgtta tcttggtatc
    10741 gctttattcg taaccaaggt ggggagatgt cttggaagtt atttttagaa gtggctgtta
    10801 aatatttcat tgcttatttc ctagtttctc aatcaggggc gattctcaat gccattttat
    10861 ggtttacgaa cggtgtgacc aaactgattg gagtagactt gagtggtgac aacatgtttg
    10921 agtttgtatg gttaaaaaaa gggaatctag gtgtccgtat tctagtaaat ggactagctg
    10981 ctatagctgg tgttggagca atgatctcta taaaagttat ggtattactt cgttttattg
    11041 aactatattt ctataaagca attgctccag tcattgtggc tttttggatg ggagatgata
    11101 ctaggcatat tgccaaaaat ttcctcaaaa cttttggggc gattgctctt caaagtgcag
    11161 taattgtttt attactagct atatggcaag gactcaatat agatgctgta attaaaatat
    11221 ctaaagattc atgggtaggc gcttttgcag caggatttag ttacataggt aagtgtattg
    11281 tctttctgat attgttgttt ggttctcagc gcaaagccaa gcagttatta caggtgtctt
    11341 gatagaccaa tcactataat atatggaagg acattctaat gaatttagaa aaactaaaac
    11401 aaagacgaga cgaacttatc cgagataatg agtggatgga cggtctcatc aaggaaaaag
    11461 aagaaaagct gtgggagctg aaagtcagaa gtatcactgc ttcagaactc gctcgctctg
    11521 ctatgcaatc agctatgcgt actgctggaa ttgttgattc tttttatgga ccatctgctc
    11581 aatcatcaga taatcaagct aacaaagcag tagaagcggt tcataataag agctgttttt
    11641 ttatgctaga aaggaaaagc gatagaaatg attaatcaag gtcaggaata ccaatatttc
    11701 aaagataaaa ttagtcattt agaaagggaa gtgagccgat tgtctcctta tgaatacgaa
    11761 caccggctat taaaggatgt tatagctgat tgtctcttac aaggacagat aacagtaagt
    11821 gaattgccac aagctattcg cttaatacaa ggcgatgatc ttttttacac ctatgcttgg
    11881 cggtttgtgg aagctacggg agattgccaa gcaggtatca ccatcttaaa aatacttcaa
    11941 gatgatttga actatttctt tgcaattggg aaactgtctc aaaaacagta ctcacagtgg
    12001 ctggagaagt ggctaagttt tttagaacgt ggtaggatag cttttaaagg agagaaagac
    12061 tttgaacgct attttcaaga ccaaaaagaa gctaatcgaa gcttattcag tgatttcaac
    12121 ttatgatagg cagttaagta aaaagcagtt tctttgaact gtttttttca tgcaagaaag
    12181 gagagctatg aacaaattag gaagtgaatt ttttaaggct atagatcaat ttgagcgagg
    12241 tattatcgga agctttactt ggagacaaat tttgatgttt tttggaattg tcttgggggt
    12301 tggacttgca acagttatct cattgctgca gcttccaagt attctctttt acctctcact
    12361 ggctttaaca gttgtcccag cctttatcta tggcatcaaa aaagatgaac acctcaaaga
    12421 ggtcatcacc tttaggctca aggtacaaga aagggcctac caaaccgaat atgaaagtga
    12481 ggaaaactat ggtgcgttta ttccccaaaa gggtgtccag gaatggaatg accctaacga
    12541 aagctgataa agaacgtgca aaacaattac agaaagccct aaaggctagt acccaaaata
    12601 cgattcaata cacgtccctc tttgaagatg ggctcatgca cattgtcgat gaagcctatt
    12661 ccaagacctg gcaattaggt gatacgaatt acatcacagg tagtgaagac gaaaaactag
    12721 atattattga atactatgtt gatgctttaa atggtctaga tagtgaaaat acctatcagt
    12781 tgacgattct taatcgtcct gtccctacaa ctttactgga tcagattacc tatgacttag
    12841 aaggtgatga gcgagatgtc tttcggaaag aatacaataa tattatccgc tctcgctttg
    12901 cgaaagatca aaataacttt aaagtggaaa aatttgtgac ggtctcgaca agggctaaag
    12961 atcggaaaca agcttaccgt aagttaaatg atgttgacac ccatttcaca acacagtttc
    13021 aattagttga tgtccttctc aagtcactag atggaacaga aagactcaat atctttgcgg
    13081 atttactgag agggaaccct tatctcgatg tggactataa ggatgtgaga atttcagggc
    13141 ttagcactaa gtccttcatc gctcctggtc gcctttggtt tcaacctgac cagtttagga
    13201 tggataagaa atattgtaaa atcctctttg ccagaacttt cccggccttt cttaatgacc
    13261 gactcattaa atccttaacc gatattggca ttgaattatc tatttccatt catgccaagc
    13321 cttatgacgt ggctgaagca gtgaagaaag tcaatacagc tgaagctggt gttaagatgg
    13381 acatggttaa atctcaagta gccgcagctc aaaaaggcat ctcgggaaac ttagcagttt
    13441 ctagtgtggc ccaagatacg gcagatgaag ctgagaaatg gaaaacagaa attaatgata
    13501 acgaccagaa aatgttttct ggcgtttttt tggtcatggt aaaagctgat agtgccgaag
    13561 aattggcaga ctatacaagt cgcatcaaac aggctggacg caaacacatg attgagtttg
    13621 aggaaattta ctatcaccaa gaggaagcgc tcaatactat gctgccgatt ggaaagactt
    13681 atatagatgt gaaacgtcgc tttatgcgag atatgaccac taccaatatt gcgacacaaa
    13741 ttccctttac caacacggac ttacaatctc aaagtccact agctaattac tatggtcaaa
    13801 atcaaatctc taataatatc atctctcttg accgacaacg ggatttatat acgtcttcag
    13861 gtgttattct gggttcttcg ggttctggaa aatcagtttt tgtgaagatc aatgaagtca
    13921 ttccagctat tctacgcttt ccgaatgatc gggtcatcat agttgatccc gaggaagagt
    13981 acgtcgatat cggccgtgcc tttggcgctc aagttattga tatctatcca ggaacaaaaa
    14041 cccactttaa tctcatggat attcctgacc tagataaact tcgagatgaa gataaagatt
    14101 ttgtagggca aaaagcatct cttatcatgg gattgtttga aaatatcctt caagaagtga
    14161 ctgacgatga tgtctctctg attgaccgtg tcactcgcct gtgttatgaa caaattactg
    14221 atcgcacccc aaccctaaag gattggcatg atattctctt ggaacaacct gaagaggaag
    14281 cgcaaagtct cgctttaaaa tcggaaagct atactaaagg atctcaagat atttttgctt
    14341 atgaaaccaa cgtagacctc aataaccaag tcgttatctt taacttaaaa aaattatcag
    14401 gtaagctaaa accctttgcc atgatggtga ttcaggatta tatttggcaa cacgtcgttg
    14461 atcacaaaga tgagtttgtg actagggttt actttgatga gatgcagaat caatttgaaa
    14521 cagatgacca agctgctttc ttttctaaaa tgtatgcacg tatccgtaaa tacgggtcaa
    14581 tcccaacagg gattacgcag aatgtggaaa ccttgctttc tcggaaagaa gggcgtaatt
    14641 tactttataa tagtgaattc ctcgttctct tgaaacaaaa gaagacgact attccctatt
    14701 tgcttcaaac cattaactta accgatgctc tcgttcgcta tattgaaaag ccaaaagcga
    14761 ttggaacggg cttaattatt gcaggtacaa caactgtccc atttgaaaat cctattcctg
    14821 aggatacaga actattccgc ttagttgcaa cggatgctta tcgaaaaatg gaggaagaaa
    14881 atgtttgaag atgtcacttt acaattgcct aaaaatgaag aaaagaagtt gatggctcac
    14941 tattatcaat tacatcagga agccattgaa gaagctttag ctggcaaaga attatggaaa
    15001 ccctacattc gcatgtctgg actatctcaa tggctagatg tttcaacgac aactattgtt
    15061 aaatggcaaa gggaaggcat gccccatatt gtcatcgatg gggtaacttt gtatgacaaa
    15121 cgaaaaattg caacatggtt aaaccatttt gaaagaaata aaatcatctc tcaagaaaag
    15181 caaatgaaga ttttcccagg ctacttatca atcaagcatg tatttgggac aattattacc
    15241 tattctaaga aagtcgttct agatctaatc caagatctta gagatgagga tattcaagac
    15301 ttttcttatt ggtccccact gtacttttta gaaacggatg aactaggcca acctgtttat
    15361 ctaacttatt tagtgaatga ggtaggtata ccgctatatc cagactgcta ttactacatt
    15421 acattaacaa aagaacaaga aagcaaatta gaaagtctag aactatcaat ttaacaagaa
    15481 aaaaggaggt tagcacgatg agtgcagttc caatcgctgc agcgtatcta gtacgaaaaa
    15541 gacaagaaga gaaaaaggta cgccaaaaga gtgagaagga gctaaaaaaa ataaaagtta
    15601 tggtaactgg tgaagagccg gtctattatg gacatgcttt gaagtcgaaa atgaaaacag
    15661 aaaaatactc tcgctcgcca gttattggag agtatgccct aaaattatcc aagaagcatc
    15721 cagggaaagt aacatggata gatattgcgg tgttattggg tctaatactt gcaggactag
    15781 ctacgattgg agcgtctatg ctgttattca tgatactttt taaaggagtg acatattaat
    15841 aaagaaagga ggcaaccgtg acagaagaaa aacaagatct tagaagtcga gttgtaagaa
    15901 aacgtcagat taaagcaagt gtccacagac ataaccagtc ttccgctaga aaggctcaaa
    15961 aagcagcaaa aggtgaattt caaatagcca ctaaagagtt aaaagaagct agagaagcct
    16021 atcaaaaagc acaaacagac tttagggctg gaataataga cgacgaagct cttcaaaaag
    16081 ccaagaacag acttaaacaa gctcaaaata agtataaaga tgctaaaaag gtcaaaaaga
    16141 aagtacgcaa aaccaatcta actcttagtc aaaaagccag gcagacgggt aggcgtatca
    16201 ccaaacgaac aggtcaagaa atcctagaaa tggggttaga acaagatgat actttatctg
    16261 atttggtgag gactaagcat cagattcgtc aaacccgagt catggctaaa caagcaggac
    16321 gtgcaaccgg taaaagtgtc aaattgactt atcgtggtgc caagggaacg gtaaaactag
    16381 gagctaaagg aactaagtac gcctatcgtc aaacacgaaa aggaagcgta aaagctactc
    16441 aagcaagtct tagagcagct tctaaaaccg ctcaaactct tgtggctact tcaaaagctt
    16501 tagtgagcgc cattacgccc attattgcta atccgataac ctgggttgtg gctggagtta
    16561 ttagcttgct tttgttaatc gttatcctgg tatcttcggt cttttcgagt aatgtggtcc
    16621 aacaaaatga attcactctc aatcaatcct ggctccatat ctctcaaatg gatcgcaaga
    16681 agtcttctga taaggtggat tactataccg atattgattc cattttactc tatatgaatt
    16741 accgctatgg tggagagtgg gaacctgatg ccaagtggga tgatggtttt ggagggaaag
    16801 tcacaggtgc cttaggtttc aatcacttct cagatgccct agatgatatc tggaaagagg
    16861 aaaacagtga tatcaccaac ctaaaaacca tggctgaact ttatactaat gggaagaaat
    16921 ggatcaagtt ctcaaaagat gacttagaag agtataagga gattctagat agccaagtcg
    16981 aaacaggaaa atatttagcc tatcaagaac tttctaaccc cttctattca tctaatgatg
    17041 acaaagcaga ttcagaatac ttgactgtga caaaacgcta tggctatacc gataaagata
    17101 ccattgatcc gacgacaaca cttcaagcaa aagccagtca aaagttgtat gcggttatgg
    17161 atgggaaggt aactgttacc aaaaaagact taaagggtga aaaaacgaaa accactaatc
    17221 tcattattga aggtaaggag gcccgcttta tttattatga tatctcaaat agtcgggtga
    17281 ggacaggaga tagggttgaa acgggtcaag aattagctac agtatcagga agcacccaaa
    17341 aaatagcgta tgctaagcgc tacggagaaa tagatgataa agatgctaca tggttaaaaa
    17401 agattaggga tagagaggtt tcttacggct atacgataca aacaaagatg aataagaaag
    17461 aagtctgggt acttaccaac ccaggctttt attttccctt tgtgaagtat gcagaaacaa
    17521 ctattgtgac tcaaactcca tctgaaatga gtggtcgagc caagcaattc tatgatttgg
    17581 ttaaaaaata tttgccccag gcaaccgata atgggattgc agctattgta ggttgctttg
    17641 gagtagagtc tggcatcaac cctaagcgag ccgaaggtga ttatctcagt ccaccagtag
    17701 gcgcttctga ttcttcttgg gatgacgagt cctggttaac gttaggtggt ccagctatct
    17761 acggcggtgg ctatccgaat attcttcatc gaggtcttgg tttaggacaa tggacagata
    17821 cttctgacgg tgcgaatcgc aacaccttat tacgagagtt tgctaaaaaa gaaggccaaa
    17881 aatggtatag cctagaactt caagtaaagt tcatgctaga gggagataat ccctattata
    17941 tcaatacgtt taagagagtt gcgactaata atggggatgt agatagtcta acagctgagt
    18001 tcttagccaa atgggaaggt gtacctggca ataaattggc tcaacgtcaa gctatcgcta
    18061 aacaagctct aatgtggttt aaacagccaa cacactcagg tggtgcaagc actcttcaaa
    18121 gttcttggaa ttttcctaaa gaatatgctt ctaaactaaa gagttatcca tcggctgcta
    18181 ctgtatctag gtcactaaat ggcaatactt atccaattgg tcagtgtacc tggtatgttt
    18241 ataaccgcct ttcagaagct ggtgctccag ctattaactg gttagggaat ggccaagatt
    18301 gggttagaaa tttagtcgct aaagggtgga cctttagtaa taaaccagtg gcaggtgctg
    18361 tttgttcaac tgctggtggt tttgactaca ccatgcctac ttatggacac gtatcctatg
    18421 ttgagcatgt caatgaagac ggtacctttt tagtgagtga atgtaatgtc atggggattc
    18481 aaaaccagat tcattgggca gtgaaattga atcaaagcta ttacactttt gcaatacctc
    18541 ccaaataaat agaaacgagg caaaataaat gattgatact tttaaagtca agtggtttaa
    18601 acgaggcttg agccttcttg ctctccttct tgtcttcttt ttaggacttg gttttggcca
    18661 gatgggaaac tcaagccact ccaaggatag acctaaacag acagccacta agaaaacaag
    18721 gacgaatact attacacaaa cacaggtaaa agaatttttg gttgcttact ataccaaaaa
    18781 agatttagaa gaaaaccgta accgctataa ggactatatg acagaaggtc tctataatgc
    18841 gacgatctca gaagaagata aagcccaaaa taaggcctat aaaggctttg tagttaattt
    18901 tgagtttcaa gacgctaata tctatattga ccaagtccat aaacaggtca tctgtgaggt
    18961 cacctatact aatgacttac ttcaaaaaaa gaataacaaa gacggtgctc aaataggtgt
    19021 tgtcaatcat acaaccatcc agttagttta tactaagctg aatgggaaaa atttgattaa
    19081 ccagatgagt acgattttgt taaccgattc agctaatcct tctgaagaaa ctgaaagtta
    19141 tggagcaatc gccccatcaa atactaaaga ttaaacaata aaggagttat atcatgataa
    19201 acgaagaaca tatgaatcaa accagttcat caaaaagccg tcggcagaaa tccactcaag
    19261 aacgtttaga ggataagatt aagcagctag aaaaggctca agaaagtcaa gacgctcttc
    19321 aaaagaaaat caaaacgtta aaagatgata ttgccatctt agaaggcaaa cggcaacagg
    19381 aaatcatggc cgaatatggt attgatttac aaggtttaga agccatttta gccactcaca
    19441 aagaaaaact tggaggtgag gggtaatgtt tcggattcag aatcaatctg atgggaagga
    19501 gcaggaattt cagaacttgg cagcacttct atatacatta gaaggagaag aaaagcggtg
    19561 cctgcagtta aacatgagag ctacatttta tctgtttcat ctggataaaa atgaagaagt
    19621 cctggaatcg atggaattaa ccattccctc tcaagaagac aaagaggttc aggaactcat
    19681 cggagacttt ggcttaaaag gagaaacaaa gaaattcttt tggcagcgct cgatggacaa
    19741 gaagccaatc cccatcaaaa caagcccaaa caatcatcaa gagttgccat ctaaaacagg
    19801 tgccttaacc ttgttaaaaa ggctggtgtg gttgctgccg atggtattat cggtagtgag
    19861 tatctacttg tctttggaga ccttgcagtt agttaagaca caatcgcagg tgactcccaa
    19921 agtagagacg gtagtgacca atcaaaaggc agatgtcttt tgtcgctatt tcattagcag
    19981 ttacttctct caaagtccta atttagggga ctatttgtct gagaagcttt ccatagatga
    20041 tctgaagact gataaagtga caccagtcag tgtcttatta gaaagtcagg aaataaaagg
    20101 ggatacaact cttgtgactt atgtcgttaa tatcaaagag gcacaagatc atgtatcaag
    20161 taagcgtcta acactagctg ttagggctga taagaaggct cagtatggct atcttgttat
    20221 caaaaagcta agactaaccg cttaccccta atgaagaaag gagacaaaac aaatggatca
    20281 agaacgagtt atggaaaaag gctcacgagt taccttggag acagggaaac acttacttca
    20341 gtttttggct tatgctgtta gccaaggagc taagatttat acagagcata aacaatcagg
    20401 ggagatgtcc tggaaggatt tcaatcagtt accccgaaca aaagatcata tcgactttga
    20461 agaggctgag gttaatcttg aaaaattgag aaaagaactc aaaaagtcag gggttaactt
    20521 tcactttaaa aagaatggag atggcaccaa gcaagtctgg tttgaagcta ttaatcaaca
    20581 ggtcattcaa gaagctctca ggaaaatcaa tcaggaaatt gtgtctaatc ccaaagaagc
    20641 actagataaa tacatgaaga aacctgatga attaacccct aaacaacaga ttgaaaaact
    20701 caagaaaacc tctaagaaat caacggaagc agtaaaaacc aagaagaaag gaaagggcat
    20761 ctgatggaac taaaacgtaa acgattgctt ccctatcttc tgctcagttt gcttctagcc
    20821 tacctgacac atcgtgctta tgtcctttac tgtatggcac cacaggcaga tatgaccaat
    20881 ctttttgctc cctataccta tgtttttgaa aagataacgg aaccacctta cttctattgg
    20941 aatactagcc cactagcaat tctttctgct ctagtgggct tttttgttgg cttacttttc
    21001 tatctaaaga ttagattaga tggtaattat cggcatggag aagaatcagg gtctgcttgc
    21061 tttgcgacgg ctcaagaact caaaggattt caagatgaag aatcaagaaa taatatgatt
    21121 ttctcgagag aagctcaaat gggactcttc aataagcgct tgccgtttga gcgacaactc
    21181 aataaaaatg tcttagtagc tggtctgcct ggagatggaa aaacctacac ctatgtaaag
    21241 ccaaacctca tgcaaatgaa ttcctctttt atcgtaacgg accctaaagg gttgcttgtc
    21301 cgagaagtag gaacaatgtt aaaagaaaat ggctatcaaa tcaaaatctt tgatctggtt
    21361 aacttaacca attcggatat gtttaacccc tttaggtata tgagatcaga attggatatt
    21421 gatcgcatta cagaagccat tgtcgaaggg acaaaaaagg gagatcgaga aggagaggac
    21481 ttctggaatc aggccaagct tcttttgaac cgtgctttac ttggctatct ctattttgat
    21541 agccaagtac ggcattatga acctaacctt tctatggtgg cagatttact tagaaatttg
    21601 aaacgcccta atgaaaagga actcagtgca gttgagaaga tgtttaagca attggaagaa
    21661 aaattaccag gtaactatgc ctgtcgacag tgggaactct ttaacagtaa ctttgaggca
    21721 gaaaccagga ctggtgtctt atctattgtg gccactcagt actccatttt tgatcatgat
    21781 gctgtaacaa atctcattaa gactgacacc atggaaatgg atacttggaa taccgaaaag
    21841 acagcggtct ttgtggccat ttcagaaact aataaggctt atagcttctt ggcttccaca
    21901 ttctttacag ttgtttttga ccaattgact catagtgcag atgctattat tcaaggggaa
    21961 aagaaaggct atgaactgaa agatttatta cacgtgcaat tctactttga tgaatttgcc
    22021 aattgtggta agattccaca ctttaacgaa gtcctagcct ctattcgtag ccgtgagatg
    22081 tctataaaaa tcatcattca agccattagc caactagata gcctatatgg cgttcctgct
    22141 agaaaatcca tggtcaataa ctgtgcgacg cttttatttt taggaaccaa tgatgaagat
    22201 actatgaaat acttctccat gcgagcagga aaacaaacca ttatacaaac cagctattcg
    22261 gaacaacgtg gacatcggat atcaggaaca accagtagac aagcgcatca acgggatctc
    22321 atgaccccag atgaaatagc tcgtattggt gtcgatgagg ccttagtttt tatctccaag
    22381 caaaatgttt ttcgagataa gaaagccatg gtatcaaatc atcctatgaa ggatagactc
    22441 gctaatcatt atcaagataa gacctggtat cactataaac gctttatgga agaaggtgcg
    22501 gaatttattg atgcagtcat gacaggacaa attccagaag accgcatttg ggctcctgat
    22561 atgtctgact accaagcctt cttagaagaa aacggctttg ataacacacg tcaatcagct
    22621 cctgagacgt cagttcataa ccaagtccct gtaactaatc ctgaaatgcc ttcagaaagc
    22681 ccctctcaga caccagaaac ctctacgaaa ctagtggata tggatactgg agaaattttt
    22741 gaacttcccc ctgaagaccg tgaaggtttc ggagaggttt cttttgatga ccaacgagtg
    22801 gaaatctagt atgatgttac tcatttcgta ttaatagaaa ggattaagat atgacacctc
    22861 aaattttacg tcgattagac gttaaaaaac agtttattga agcaatagac ccctttgtcc
    22921 accgtcaaac cctaaagccc aaagccgtga attcatctaa aaccacaatg tcaattcaac
    22981 gctataacca tgcgggtact aaaattcagt tacgaatcgg ctatagtaag gtactcatcc
    23041 gtatcttctc caatgggaaa atcaatctca cccattatga tctctttttt gaccgagaag
    23101 aaactctgga gataactgat gcctttgaca atggtgttta cacgcaagat gaggtagatg
    23161 gctttatcaa acaagccaaa acctttatca agcaagccct taagggggaa gtctgatatg
    23221 gcgaatacat acgatttaat ttcacagcgt attgaagcac aacgacagaa actcatcgct
    23281 atagatattg tggccgttgc gagctcacta ggacttaact tgaaacaagg ttcaggtgga
    23341 catctctatt gggatgaaca tgatagtttt catatttacc cccaaactaa cacctttcgt
    23401 tggtggtcaa gaagcatggg gaccaatacc attgatttag tccaagtcat ccaagaagag
    23461 ctaacaggaa agaaacctaa ctttcgagaa actgtcaact ttttagaaac agggcaattt
    23521 gaatcggtta cggtaacacc agtcgttaga gaacccttta agcactacct cgctccttat
    23581 gaacatcaca attttgactt agggcgacag tatcttaaag aagaacgagg gctatctgat
    23641 gagaccattg attttgcttt ggcatcaggt agtatgagct ctgcgacatt gaaaaagggt
    23701 gattattttg aacctgttat tatctttaaa agttttgcgg aagacggccg aatgatcggc
    23761 ggtagccttc aggggatcgt tgaaaataaa gtccagcatc ctgaacgtgg ccgtctcaaa
    23821 caaatcatga agcattcaga cggtctagca ggttttcatt tggacgttgg aacacctaaa
    23881 cgtctcgtgt tttcggaagc tcctattgac cttctctctt attatgaatt acataaggag
    23941 agtttacaga atgtccgctt agtcgctatg gatggggtca aaaaaggggt gattagtcgc
    24001 tatacggctg atttattgac agatggtcag tattctcaaa ccatgcccag agagtcgatt
    24061 agaggagcga tagatgccat taaccaaacg accagaatcc ttaaaaacaa tcccaatctg
    24121 attaccattg ccgttgataa tgacgaggca ggccgaaact ttattaagga gttacaggaa
    24181 gatggcattc caattaccat agacctccca ccacggaaag aacaccagag taaaatagat
    24241 tggaataatt atctgaaaca aaagaaagga ttactaaaaa tgccacaaac agaaggcaca
    24301 caaaaagctc ttgatattac tcaagagcct aacactgggg gcgaacgttt taatcgcaat
    24361 tccagttttt taggagaaga atcttctcca aggacagcac cgaagcctgt tgtggacgaa
    24421 ccgcaacctg attttcctac caatgttcct tttcatttta gcattgatag gtctaaaaag
    24481 tcaaccacaa aactgcgtag cagctatgag tatgcctcag acaaacaatt acggcagctc
    24541 aatctatttg cggatgatat cagaaaatca gctaattggt atttagatac tgtcgcagat
    24601 agtaaagtaa cttattttta tcaagatgga gatgaaatta ctactttaca ggtggaattt
    24661 gagaagaaaa attggatgca tttaacggga cttgctcctg tttattctaa ttgggttcct
    24721 tccctgtctg aaagttttat cgaagatatt gcaaatggga aagggcagtt tgcgaacttg
    24781 acattaggac gtggggtaat ggagaaaata caaattttac caatacttcc tgaagtgtta
    24841 gagacagatt cttttgtttt tgatgactta tccagtgttg aaaaattcca acgcttagat
    24901 gcaaacaaag caattataac agacaatgaa cagttgatat tggctttccg gtttgatgac
    24961 gggaattttt atccagctac attattgaag ccaacgaaag cactaaatat tcagctgaat
    25021 gaaacgaatc aagaaaaaat cgttctaggt gtctttattg agaaagacgg attagttaaa
    25081 acccataagg taaatgaaga ctatatcaaa gatggtggga atgagttgaa gtcaattatt
    25141 gaaaatgatg aactagaacc cattcaaaca gcccctttgg agcgaaatgt acaacaggaa
    25201 aaaatatttc caaaggattc tgacggtgat ggcttaaccg atgatgaaga aatcgctcta
    25261 ggcaccaatc cctttagttc tgatagtgat ggtgatggga ctccagataa tgttgaaaag
    25321 gcaaatggaa cagacccaac caacgcttct gataatgagg tgacaaggca acaagaagtc
    25381 aataaacgtg atttcacttt gtcagaaatg attaaagcca aaaataccgc agcgctgaat
    25441 caacacttac aagacggcat aaaacagtat tttgatagta atacctataa gcaatacctg
    25501 gaagggatgg ctcacttcaa taactactcg ccacgaaata ttcaattgat tatgtcacaa
    25561 tttccagaag catcgatggt tgcgtctttc caagaatgga agaaacgtaa tggctctgtc
    25621 aataaaggtg aaaaagcaat ctacattcaa gcacctgtag ctgttattca aaaagataag
    25681 aatgggaaac ctattcttaa tcccgaaaca ggagaaaagg agaccatcac ttactttaaa
    25741 cctgtccctg tctttgacat taaacaggtt tctccccaag aaggaaaaga actcaatctc
    25801 cctaaagcta tgggaactat tccagaacaa ttggataaag actactatca aaatgtctat
    25861 cgtagcttaa gagatatttc tcagaataac aataaggttc cgatccgctt tagggagctt
    25921 ggacaagaag atgggttcta tagtccacaa acaaatgaaa ttgttattaa gaaaggaatg
    25981 tcttatgaac gaacgttatc aacgctgatt catgaaatgg ctcattcgga attacacaat
    26041 aaacaaagtt tgacggaacg gtttgatggc cagttaacaa gaagtacaaa agagcttcaa
    26101 gcagaatcca ttgcttacgt tgtgtcgaac catctaggat ttgataccag tcaagagtca
    26161 tttccttatt tagtgtcctg gtcaaaagaa aaagacgggc ttgccaattt aacagcacaa
    26221 ttggaaatcg tgcaagaaga agccaaaagc ttaatggaac ggattgatca acagttaagt
    26281 caatatcaaa cagtaacact gaataaagaa acccaacaat tgactaaaca agaaatgaaa
    26341 aagcaggctc atccctttta ccaaagttta gcagccgcta aaacatcaag aacacaagtc
    26401 accactcagg aaaaggaagc aagcgtaaaa aaagacaatc gacctacgat gccgtagtgc
    26461 aacgtaggtc atcacagaaa gcaatgatat tatcaggagg acaaagatat gttaaccgaa
    26521 gagatgatga cgacaactga aatgattcta atgacctact tatttgagat agacgagtgg
    26581 ctcaaagaca agaaagtcat ccagcaacaa caggcacaac tgacaaaaga ggaactcgta
    26641 acggtcatta aaaacgattt gacaacgttt caagctaacc aagccacaga ttatgagaag
    26701 aaacttcaga caacactgac aactctagaa gcattgtctg aagcccaatt tcaaaaaatg
    26761 aaattagaga tattgtcttg ggggccaaac gtaaaacacg tcgattgaca caacataaga
    26821 agcggacaat gtaatgattg tccgcttttt actgtgtgca tttttcaaga ctgacactct
    26881 taaaagttgt gccattgatc aaaatgtaag atcggaaaaa agtgcaacat gacaaagtgc
    26941 acgtcgtgcc aaaaagggga ttggggattt ccccaaaaag taaaaaggca acagtctaat
    27001 tgtgggattt ccgcaaatag gcttgtcgcc ctttttattt tagcatgtgt ccggacacgt
    27061 gctaatcttg ccagagaaca agcctactgg actctgggaa agctgtcaag cccttagtaa
    27121 gggtgacaat atagaaatga ggtattgaat atgataacac caagtgaatt aacacaccgc
    27181 attgaacaca caacattatc agaagcgatt gagctttttg aagataaggt gcttagaaag
    27241 tctctaaata actacgacga ctggtataaa cgagacgttc aaaaggaata tgaacggatc
    27301 aattatgatg gtgcattctt cttttttgtc gaaccggatt taggttctag tcgaggtggc
    27361 gtatctgatg tcatcataga agaacaagaa aaagtagcct tattgttatt attggttgag
    27421 gcttatgagc gttatattga tgtcaacaca gggatcaaag attggttagg ttatgattgt
    27481 atcttttgtg acgttgttgt atccaatgag acggccgcta aacgcttaac acaaatggag
    27541 tatgaggcaa taaaagattt aatcgtaacg gtcattgatc actatgtccc aagtatgacg
    27601 gtgatggaaa cagatgaata caaagaattt aaacaaggac aaacgccaaa tgatactgtt
    27661 attgataatg tccaaattac tttaccgcta tttaataaac gggagaagtg aagatggtag
    27721 agaagagaag aacacgaata aatagatttg agttacgaac aactgatggt gaagctaata
    27781 agttacgtcg tcggattacc ttatcaggaa agaaaacctt tcaagcttac gctttaaaga
    27841 tgttacttga gggtaaaatt gaaacctatg attattccga attgcgtcaa ctacgaattg
    27901 aggtcaatcg tattggtcaa aatatcaacc agctggttcg ttatgtgaac acctttgagg
    27961 aatttgacca ggagctattt acagctttac aagaagaagt caaagaactc caacagatga
    28021 ttgtccaaga atttaaaaca aaggaggttg ccaaacaaca tggtagtgac caaagtcttt
    28081 cagattaaac agattaaaaa cttaaaacga gctattgatt atatcacacg tgatgatgcg
    28141 acgcttatca aagttgagca acatgatgaa gatgatgctt ttgattatac gctaacggca
    28201 gatggtgagg ttcacaagaa attgatttca ggccaccacc tcattgaccc aggagaccgt
    28261 gatgccattt atgatgattt cattttattg aaacagtctg tcgatgaatt gtacgataat
    28321 gccaccttgt ccgatttgaa aaatgataat cgggtcttgg cgcatcacat cgttcaatcc
    28381 ttttcaccag atgacaagct aacacctgaa gaagttaacg agattggccg gaagacagct
    28441 ttagaattta ctggaggcga ttaccaattt gtcgtagcga cacatgttga taaggggttt
    28501 ttacataatc acattatttt taacacaacc aatgaggtaa cactgaaaaa gtttcgttgg
    28561 caaaaaaata cagcccgtaa cttatttcaa atttcaaata agcatgcgga gttatacggt
    28621 gctaaaattc tagaacccaa attaagaaca tcctatacag actattcggc ttggcgacgt
    28681 caaaacaatt ttagttacga gattaaacag cgtttaaatt ttctcttaaa gcactccatt
    28741 tcaatagagg attttcaaca gaaagcgaaa gcccttgatc ttcacattga cttttctggg
    28801 aagtttgcca agtatcgctt actggttcct ttagatggta agttgcagga aaagaataca
    28861 cgagatcgca ccttatcaaa gaaaaggatt tactcctttg aacaaatcca aaagcgtgtt
    28921 gcgatgaatg aagtggttta cacggtagac catatcaaag aaaagtatga ggaggaagaa
    28981 gctaaaaagg cagaagattt tgaaatgaag gtgagaattg agccttggca aatttctgag
    29041 atcaccagac agtctatcca tcttccggtt acttttggct tagaacaaaa aggaacagtt
    29101 tcgattccag ctcgactgtt agaccagaat gaagatggaa gcttcacagc ttattttaag
    29161 aaaaaggact atttctattt tatcaatcca gacaagtcaa cagataatcg cttcatccaa
    29221 gggagtactt tggtaaaaca attgtctctg aatagtgggg aagtgattct cactaaaaat
    29281 tgggaaatga cagagttaga aagactggtt aaagagttta actttttgtc ggccaacaag
    29341 gtaactaatt ctaaacagtt tcaagagatg caagaacgct tcttagaaca attagaaaaa
    29401 acggatcaaa cactggcgca gttagatagt aaggtggctt atttaaataa agtgttagga
    29461 gctttatcag attaccaaga tggcttggtc ccatctgaag tcacgatggc aattctagaa
    29521 aaagctaaag tggctaaaaa tacggatcgc aatcttatcc gaaaggaaat caaagaatta
    29581 caaatagagc gtgaggcact tcaagaagcc agagatcgta ttgtaaaaga ctatgacttt
    29641 agccacaaac tcactcaaga atacagccct aaagagagtc gaaagaaatc gattatatag
    29701 tatactaaaa caaaagaaaa ggagaagtct atctgaaaag gtgagaaacc tgttgacgtt
    29761 ttttcaaaaa taggtaaatc attaacattg tatcgacaac ttgccatgct ataataacac
    29821 caaggagata ttgactatgg gaaagagcta ttactatgtg gaggtggaaa ccatacaagg
    29881 agagtcactt atttttcaat tgccaaatga tttacaaggg gctatgagag attatcgtca
    29941 tgagaatccg gagacttggg aagcattact gaaagaagct ttgatcaata ttccaagcgc
    30001 ttcttatacc aaagctaatc attatcagcc aatcattcgt ttggccaagg ttaaaaaaag
    30061 tttctctctt aagaaacaac agcgtagacg gagccgtggg caattcctaa ccaaagataa
    30121 ttggcaacaa aagggcttca agcatttcag ggatagtctt cgctttattc aacatgatta
    30181 tccttttaaa attagagtga ggttattgct tgatttttgg cgttggaagc gacgctcata
    30241 ttaaaagaat tagtatttct agtataataa tcctatgact agatttgatg agaaaatttt
    30301 tgtgattgaa atggctaaac tacgtgagaa ggagtttttg aaacggatta gggattatga
    30361 cgaacaaata gccacgacaa tgcgccaaca cggctacaaa cgagtggata gtagcgagag
    30421 aacagttctc tttacttttg gagaaatcac ttttgcaaga aatcgttggc gaaaaggaag
    30481 aaagacccgc tacccagtag atgagtggtt aggactaaaa ccctatatgc gctactctcc
    30541 tgaattgatg cttcatatgg cggaacacgc ttctaaatta tcttatcggg aagtttgtcg
    30601 tacaattgaa acagcttatg gcctattagt gacaaaagat gctgtcttaa aagctgtcaa
    30661 actagcagaa cggctcttaa tagagaaaga acactatcgt ttcttacagc acgtagaaca
    30721 tccacaaaaa atccaagcag aaagaatcta cttggaaggg gatggtgtca tggtcaaaac
    30781 aacctctgga ggagatgagc gtcataatac agatttggct cattttttaa tccatacagg
    30841 atcaagaaag agtggtaaaa atcgctatag tctaacaaat aagcatgaaa ttattaatgc
    30901 taactatgat aaagcaagag aggagctatt agactatctc tacaatcact ttgaaataac
    30961 ggataagacc atcctcatta cgaattcaga taacggtaaa ggctatacca aacgtgtctt
    31021 tcaagaggtt aagaaagcac ttggtattaa gcatcatgaa catttttggg acagctatca
    31081 tctcaataag cagttgaaag attttttcaa gccctatcct tatgtcctac aggatttagc
    31141 ttttaaagca attcaaactc ataaaaagtc attattagaa actgtctttg atacagtaga
    31201 aacactgata gaaactgagg aagactatat taactattat gcttttaggc agaaactctt
    31261 tagaaatttt agggatacta ggccatcaca attacgaggg ctaacctctc gtggtatagg
    31321 tgccatggaa agccaacacc gcaaagtgac ttaccggatg aaacatcgtg gtatgtattg
    31381 gtcagtaaca ggtgcttgta ccatggctaa aattatcttg ttggaacgga taaataaact
    31441 aggtgacctc ttctttggcg actggcgaaa gcagtacaag aggtatcaag gaagaggttt
    31501 aggtgctggt catttggtta accactatcc tcatgatgca gtgcttatca ggcgatgact
    31561 acaaaaaaat ctatcctata acaaccgcta aaaaatttaa ctttagaggc ttttttagtg
    31621 tgtttatgcc ccgaaaccct tgcattactg ggttgtttgt ggtacaataa tagttagata
    31681 aaatgaaaaa aggtgcagaa gcgcaaactt ctacaccacc gattaagaca aaaaaggact
    31741 ttgcttaatc agtcaaacag tattgtatca tgttataaac aatatcgtca agactgaagc
    31801 agagaaaacc tttgtttcag tcttttttgg aataaaagaa cctagagttt tcttaggtta
    31861 taagggttag ttggactttc ccgaaaattc agacacatac taatggctta aaactattgc
    31921 ttttatttat gattatagta taatggtaag gtatgttata aacatgcctg tgggaggtaa
    31981 aaatttctaa ttaccgccaa aaccacaaca ggaggatttt taaaaatggc taaaaaagtt
    32041 gaaaaacttg taaaacttca aattcctgct ggtaaagcaa ctccagctcc accagttgga
    32101 ccagcacttg gtcaagcagg aatcaacatc atgggattca ctaaagagtt taacgctcgt
    32161 acagctgatc aagctggtat gattattcca gttgttatct cagtatatga agacaaatca
    32221 tttgatttca tcactaaaac accaccagct gctgttcttt tgaaaaaagc tgcaggtgtt
    32281 gaaaaaggat caggtgaacc taacaaaact aaagttgcaa caattactcg tgcgcaagta
    32341 caagaaattg ctgaaactaa aatgccagat ttaaacgctg caaaccttga gtctgcaatg
    32401 cgtatgatcg aaggtactgc tcgttctatg ggattcactg ttactgacta atcagaaaca
    32461 cccacataac ccgcaagact tcatcgtagt ttgatgagtg acgtgggaga ttttaaatca
    32521 aatcgattga ccacattaca aggagaattt taaaatggct aaaaaaagta aaaacttacg
    32581 tgctgctctt gagaaaattg acagcactaa agcatacagc gtagaagaag ctgtagctct
    32641 tgcaaaagaa actaactttg ctaaatttga tgcaactgtt gaagtttctt ataaccttaa
    32701 cattgatgtt aaaaaagctg accaacaaat ccgtggtgct atggttttac cagcaggtac
    32761 tggtaaaact tcacgtgttc ttgtttttgc tcgtggtgct aaagctgaag aagctaaagc
    32821 agcaggtgca gactttgtcg gtgaagatga cttagttgct aaaatccaag gtggatggct
    32881 tgactttgat gttgttatcg caacaccaga tatgatggca cttgttggac gtcttggacg
    32941 tgtccttgga cctcgtaact tgatgccaaa ccctaaaact ggtacagtaa ctatggacgt
    33001 tgctaaagca gttgaagagt ctaaaggtgg taaaattact taccgtgctg ataaagcagg
    33061 taacgtccaa gcccttattg gtaaagtttc atttgatgat gctaaattag ttgataactt
    33121 caaagcattt aatgatgtta ttgttaaagc taaaccagct actgcaaaag gaacttacat
    33181 cacaaacctt tcaattacaa caacacaagg tgttggtatc aaagttgatc ctaactcact
    33241 ttaatcaata aaaaactagg aattccttcc tagttttatt tattctatat acttaactag
    33301 ttaagtatat attttaataa tcaaaggata ttatatgtgt ttcttttgaa aaatagcttt
    33361 ttatctgtat taaccttctt aaagactaat gaatgtgcga tgtgttttaa gtaacagatg
    33421 agaataagga aaaatgaaat tggcgatatg gtatgtgact attatgatta ttctttagca
    33481 ataatctatt aaaatgagtt cgcagggaat aagtaaattt tgtatcgttg tagatt
//
LOCUS       DATRQA010000005        31007 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.005, whole genome shotgun sequence.
ACCESSION   DATRQA010000005 DATRQA010000000
VERSION     DATRQA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31007
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.005"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..550)
                     /locus_tag="VBD27_000184"
     CDS             complement(<1..550)
                     /locus_tag="VBD27_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024228.1"
                     /translation="MDKLKKLQKENTLLEGRIDNSNNQTYTDMIVYLRGASISPYHQE
                     LIRNDIVNMLLEAQERQASLVSVFGEDRHDFINQVIKSTPKISKKEETLQRWDLAILL
                     LTIQMIIFLGGYLITEALQQSVPDLIPITLLDVLFAIFISIIAVKITDTIIYATYNFD
                     KSKEKKYFFRYMFLILSLIIAYI"
     gene            complement(571..882)
                     /locus_tag="VBD27_000185"
     CDS             complement(571..882)
                     /locus_tag="VBD27_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO7024229.1"
                     /translation="MIPSQMLKGLLEGAILKIIQQEETYAYEISKKLNSYGFGEISEG
                     TIYPIILRLQSNKYIKGQQKPSPQGPKRKYYTLTNEGVEQLAQFKQNWESLSKALNKL
                     L"
     gene            1073..1780
                     /locus_tag="VBD27_000186"
     CDS             1073..1780
                     /locus_tag="VBD27_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEO7024230.1"
                     /translation="MKIDKKELLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVN
                     HVSDSQLTEAQDMLDKFEKKPSEKLLKDVELALNKLSNSSKKEALQKRFKKAKDKYLK
                     DEADKKATKDATDLVEILEQAPSEENVLKAEAAVKKLTVKESKEALQKRIDTVKTQYG
                     LIGNQTPSSSVAETTEQGTANPASQDTSSYVNQDVAPTYEQPQTNNTPVTPGVNNTVP
                     TPGTGTVPATNGTGVAQ"
     gene            2058..3206
                     /gene="nagA"
                     /locus_tag="VBD27_000187"
     CDS             2058..3206
                     /gene="nagA"
                     /locus_tag="VBD27_000187"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEO7024231.1"
                     /translation="MTKYIKADRFFYADHVKENGYLEIKDNHFGKWIENISGQEEILD
                     YSGYQIAPGLVDTHIHGFAGADVMDCDSEGILRMSAGLLSTGVTSFLPTTLTSDTKRL
                     EEASKAVAAVAGKEQGAKIQGIYFEGPYFTEEYKGAQNPIYMRNPNLEEFAQWQKAAK
                     GLITKIALAPEREGVEEFVSAITKQGVTVALGHSNGTYKEAKKAVKAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVDPVACDILMTQKGHNHVALITDCMAA
                     GGAPDGDYMLGELPVVVSNGTARLKDGGNLAGSILKLKDGLKNVVEWGIATPEEAVRM
                     ASYVPAVSVGIDNVCGQIKEGYAADFIVLDKHLDLVATYLDGEKRWQS"
     gene            3324..3866
                     /locus_tag="VBD27_000188"
     CDS             3324..3866
                     /locus_tag="VBD27_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEO7024232.1"
                     /translation="MSIIIGLMAAMFIIRLAYLKLSIANEKALRKNGAKEYGVGVSKA
                     ITVLHIIIYFSSVTEAVLTKASFNFVSVIGLSLMIFSVFMLHTVTRLLGRIWTVKLMV
                     DKNHQFVDHWLFRVVKHPNYFLNIAPELLGVTLLCHAKYTALFVLPIYAFVIYLRIRE
                     ENLLLKTIIIPNGIKKSRVS"
     gene            4179..5093
                     /gene="glyQ"
                     /locus_tag="VBD27_000189"
     CDS             4179..5093
                     /gene="glyQ"
                     /locus_tag="VBD27_000189"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO7024233.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLKSLEL
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLQTGPVTSE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFTQPEYEHSKYSFEISDQVMLLENFE
                     KFEREAKRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEETRIKLLAEED"
     gene            5093..5734
                     /locus_tag="VBD27_000190"
     CDS             5093..5734
                     /locus_tag="VBD27_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7024234.1"
                     /translation="MNTLIVNSHPDFSNPYSFTTILQEKFIELYNEHFPNHQLSILNL
                     YDCVLPEITKEVLLSIWSKQRKGLELTADEIVQAKISKDLLEQFKSHHRIVFVSPMHN
                     YNVTARAKTYIDNIFIAGETFKYTENGSVGLMTDDYRLLMLESAGSIYSKGQYSPYEF
                     PVHYLKAIFKDFMAFDDFCVVRAEGTDILDRQVVLDKMNQDLREAFEAFYSKE"
     gene            5738..7777
                     /locus_tag="VBD27_000191"
     CDS             5738..7777
                     /locus_tag="VBD27_000191"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO7024235.1"
                     /translation="MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETV
                     QTFSTPRRLAVRVKGLADQQTDLTEDFKGPSKKIALDAEGNFSKAAQGFVRGKGLSVD
                     DIEFREVKGEEYVYVTKHETGKSAIDVLASVTEVLTELTFPVNMHWANNSFEYIRPVH
                     TLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEISSASSYEDDLRQQFVIADAKER
                     QQMIVNQIHAIEEKKNISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTS
                     MKNHQRYFVVRDRDGKLLPNFISVRNGNAEHIENVIKGNEKVLVARLEDGEFFWQEDQ
                     KLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQHLADKAGLSDEEKLAVLRAASIY
                     KFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRIGAI
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYG
                     LSFASLDYANQKEVMAFISARIEKMIGSKVPKDIREAVLESDTYIVSLILEASQALVQ
                     KSKDAQYKVSVESLSRAFNLAEKVTHSVSVDSSLFENNQEKALYQAILSLELTEDMHD
                     NLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK"
     gene            7789..8046
                     /locus_tag="VBD27_000192"
     CDS             7789..8046
                     /locus_tag="VBD27_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEO7024236.1"
                     /translation="MDPKKIARINELSKKKKTVGLTGEEKVEQAKLREEYIEGFRRSV
                     RHHVEGIKLVDDEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            8135..8398
                     /locus_tag="VBD27_000193"
     CDS             8135..8398
                     /locus_tag="VBD27_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEO7024237.1"
                     /translation="MIELLLALLLWNILVFATYAIDKRRAIRGAWRISEKTLLIESLL
                     LGGFGAFLGGEVFRHKTTKWYFRLVWYIGMIIDLAIIWLIWCY"
     gene            8513..10021
                     /gene="glpK"
                     /locus_tag="VBD27_000194"
     CDS             8513..10021
                     /gene="glpK"
                     /locus_tag="VBD27_000194"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO7024238.1"
                     /translation="MSSEEKYVMAIDQGTTSSRAIIFNKKGEKIASSQKEFPQIFPQA
                     GWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRETTVVWDKKTGLPIYNA
                     IVWQSRQTAPIADQLKQEGHTNMIHEKTGLVIDAYFSATKVRWILDHVPGAQERAEKG
                     ELLFGTIDTWLVWKLTDGLVHVTDYSNAARTMLYNIKELKWDDEILELLNIPKAMLPE
                     VKSNSEVYGKTTPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMN
                     TGEEMQLSQNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLRMIETSSESEGL
                     AQSSTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRD
                     VIDTMQVDSGIDIQQLRVDGGAAMNNLLMQFQADILGIDIARAKNLETTALGAAFLAG
                     LSVGYWESMDELKELNATGQLFQATMNESRKEKLYKGWRKAVKATQVFAQED"
     gene            10034..11863
                     /gene="glpO"
                     /locus_tag="VBD27_000195"
     CDS             10034..11863
                     /gene="glpO"
                     /locus_tag="VBD27_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEO7024239.1"
                     /translation="MEFSRETRRLALQRMQDRTLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVTNTPAANKVLSAEDVLKREPDLQKEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGAYIASHVKAEDFLFDDNNQIIGVRARDLLT
                     DQVIDIKARLVINTTGPWSDTVRNFSNEGKQIHQLRPTKGVHLVVDRQKLNVSQPVYV
                     DTGLNDGRMIFVLPREDKTYFGTTDTDYHGDLEHPTVTKEDVDYLLNIVNKRFPEAEL
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKLSDESFEELIDSVKDYIAHKNHREDVE
                     KAISHVESSTSEKELDPSAVSRGSSFERDDNGLLTLAGGKITDYRKMAEGAMETIINI
                     LDKEYNRKFKLINSKTYPVSGGEINPSNVDSEIEAYAQLGTLSGLSIEDARYIANLYG
                     SNAPKLFALTRQITEAEGLSLVETLSLHYAMDYEMALSPTDFFLRRTNHMLFMRDNLD
                     SLIQPVIDEMAKHYQWSDQDKTFYEEELHETLKDNDLAALKDN"
     gene            11875..12573
                     /locus_tag="VBD27_000196"
     CDS             11875..12573
                     /locus_tag="VBD27_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7024240.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKNHNSGWIVITFGWG
                     LAVAIAALVSGNISPAHLNPAVSLAFAIKGDLAWGTAILYMIAQIIGAMLGSLLVYLQ
                     FRPHYEAAENKADILGTFATGPALKDNFSNFLSEVLGTLVLVLTIFAIGKYNMPPGVG
                     TMSVGMLVVGIGLSLGGTTGYAINPARDFGPRLLHALLPMKNKGDSDWTYSWIPIVGP
                     MVGAILAALIFAMM"
     gene            12661..13927
                     /locus_tag="VBD27_000197"
                     /pseudo
     CDS             12661..13927
                     /locus_tag="VBD27_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            13918..15348
                     /locus_tag="VBD27_000198"
     CDS             13918..15348
                     /locus_tag="VBD27_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7024241.1"
                     /translation="MRIEALMEKERRVQYRLLSFLRGSPQAIALKLALLETGLSRATL
                     LKYINNLNSYFEQEKVNCRIVYYKDKLFLEEDYNLSNQEVLKALMKDSIKYTILISLF
                     NQRQFTIVGLSQELMVSEATLNRHLAHLNELLAEFDIAISQGKQIGDELQWRYFYYEL
                     FKQLWSYDKCQNMIKKLDLDSLILLIERLAQHTLTREAQQNLGLWFSICHHRLLAMEK
                     LSDNLKPIVEHYQCNAFYKRLDAALVLYMSRFALEYREGEVLATFAFLHSQNILPINT
                     MEYIMGFGGPIIDCVTETIIYFKKESILADETSDQVIYQLGQLYSHYYFFKGHILVEQ
                     PDLEQTYRLIDHNMRDKLHDISKKIIANVNRIRPLTEDGCSLLTLHLLELLIFSKNSQ
                     KMPFRIGLDMTGNAVEQSLLEYRIRQHFSGNNSIQVEPYDEGKGFDMVIYQSRSRPYK
                     AKLTYCLNKGASERELQEIDSLIYDN"
     gene            15473..17458
                     /gene="tkt"
                     /locus_tag="VBD27_000199"
     CDS             15473..17458
                     /gene="tkt"
                     /locus_tag="VBD27_000199"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO7024242.1"
                     /translation="MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLW
                     NKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYSLLHLAGYDLSIDDLKQFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTYTLHGDG
                     CLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHIL
                     VKDGNDLEAIAAAIEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFA
                     KKAYGWEYPDFTVPAEVAARFASDLQARGAKAEEAWNDLFAKYEVEYPELAAEYKEAF
                     AGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNTMVAAETD
                     FQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSET
                     DRPTMLVLTRQNLPVLEGTSELAQEGVNKGAYILSEAKGELDGIIIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSFGWGKYVGL
                     NGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL"
     gene            17670..17975
                     /locus_tag="VBD27_000200"
     CDS             17670..17975
                     /locus_tag="VBD27_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO7024243.1"
                     /translation="MKKECRDFYRQIQHTYNDISVREDAVLSSILLSASNGLIKTSDV
                     PRVAYELTQQLENNEIEKSFESLATVKELKKSAKKLSERYEQNKVTTGLLSKVFGNH"
     gene            17995..18729
                     /locus_tag="VBD27_000201"
     CDS             17995..18729
                     /locus_tag="VBD27_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7024244.1"
                     /translation="MIKFEHVSKVYGEKEALSDLTLSIKDGEIFGLIGHNGAGKTTTI
                     SILTSIIDATYGQVYIDDLLLTEHRDQIKKKIGYVPDSPDIFLNLTAEEYWYFLAKIY
                     DVAPEDIEARITKLVDIFELEEQRYNPIESFSHGMRQKVIVIGALLPNPDIWILDEPL
                     TGLDPQASFDLKEMMKEHAKNGKTVIFSTHVLAVAEQLCDRIGILKQGKLIFVGSLGE
                     LKMQYPDKDLETIYLELAGRQANREG"
     gene            18733..20337
                     /locus_tag="VBD27_000202"
     CDS             18733..20337
                     /locus_tag="VBD27_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7024245.1"
                     /translation="MNWSRIWELVKINILYSNPQTLSALRKKQEKHPKKEFSAYKSMF
                     RNQLFQILLFSIIYVFLFVSLDFKEYPGYFTFYIGIFTLVSIIYSFIAMYSVFYESDD
                     VKQYAYLPIKSEELYVAKIFATFGMSVTFLMPILTLMIVAYWRIIGGPLAVLLAIINF
                     AILFLSVTVISLYINSLIGRAIIRSANRKLISTILISLATFGAIVPLLFVNMTSQKMV
                     QGKLQDIAPIPYVRGYYDIVTAPFSMESLLNYYLPLLIILFLIGAIYKWVMPRYYQEL
                     LYGQVKQRKVHRQIDFSKRESINKTLVKHHLSSLQNATLLTNTFLMPLLYLAMFIVPI
                     LNNGKEIGRFFNENYFGIAFLAGILIGSLCVMPASIVGVGISLEKSNFYFIKSLPISF
                     SYFLKHKFVTLITLQLAVPTFIYFLVGFFLLKLSILVLLSFILGLVFMGLIEGQFIYR
                     RDYKHLFLNWQEVTQLFNRGLGQWLLVGSLFGMMIIGSFLIGISIFWSMVWNTVAVNI
                     IILIIGLLILSICQYLLLKNFWKKLV"
     gene            complement(20545..21931)
                     /locus_tag="VBD27_000203"
                     /pseudo
     CDS             complement(20545..21931)
                     /locus_tag="VBD27_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643962.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            22072..22875
                     /gene="proB"
                     /locus_tag="VBD27_000204"
     CDS             22072..22875
                     /gene="proB"
                     /locus_tag="VBD27_000204"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO7024246.1"
                     /translation="MKRHFETTRRIVIKVGTSSLVQTSGKINLSKIDHLAFVISSLMN
                     KGMEVILVSSGAMGFGLDILKMDKRPQEISQQQAVSSVGQVAMMSLYSQIFSHYQTHV
                     SQILLTRDVVVFPESLQNVTNSFESLLSMGILPIVNENDAVSVDEMDHKTKFGDNDRL
                     SAVVAKITKADLLIMLSDIDGLFDKNPNIYDDAVLRSHVSEITDDIIKSAGGAGSKFG
                     TGGMLSKIKSAQMVFDNNGQMILMNGANPRDILKVLDGHNIGTYFAQGK"
     gene            22885..24138
                     /locus_tag="VBD27_000205"
     CDS             22885..24138
                     /locus_tag="VBD27_000205"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO7024247.1"
                     /translation="MTYIEILGQNAKKASQSVARLSTASKNEILRDLARNIVADTETI
                     LTENARDVAKAKDNGISEIMVDRLRLNKDRIQAIANGIYQVADLADPIGQVVSGYTNL
                     DGLKILKKRVPLGVIAMIFESRPNVSVDAFSLAFKTGNAIILRGGKDAIFSNTALVNC
                     MRQTLQDTGHNPDIVQLVEDTSHVVAEELMQATDYVDVLIPRGGAKLIQTVKEKSKIP
                     VIETGVGNVHIYIDEFADLDMAAKIVINAKTQRPSVCNAAEGLVVHQAIAKGFLSQLE
                     KMLKESNQSVEFRADEEALQLLENAVAASETDYATEFLDYIMSVKVVDSFEQAISWIN
                     KYSSHHSEAIITNNISRAEIFQDMVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKLH
                     ARGPMGLEALTSTKYYINGTGQVRE"
     gene            24221..25168
                     /gene="rsmH"
                     /locus_tag="VBD27_000206"
     CDS             24221..25168
                     /gene="rsmH"
                     /locus_tag="VBD27_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO7024248.1"
                     /translation="MTNDFHHITVLLHETVDMLDIKPDGTYVDATLGGAGHSEYLLSQ
                     LGPDGHLYAFDQDQKAIDIAHIRLKKYVDTGQVTFIKDNFRNLSSNLKALGVSEINGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREQSLTAYDVVNTYSYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAEKPISTTTELAEIIKSSKSAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDLLAVDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLQPKMELVNRKPILPSQEELEANNRAHSAKLRVARRIR"
     gene            25183..25509
                     /gene="ftsL"
                     /locus_tag="VBD27_000207"
     CDS             25183..25509
                     /gene="ftsL"
                     /locus_tag="VBD27_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO7024249.1"
                     /translation="MTNEKRTEAVTQTLQRHIKTFSRIEKAFYGAIVITAIIMAVGII
                     YLQSNSLQVKQEVNQLNSKINDKQTEFDNAKQEVNELSNRDRITKIAKDAGLTIQNDN
                     IYRKVD"
     gene            25513..27771
                     /locus_tag="VBD27_000208"
     CDS             25513..27771
                     /locus_tag="VBD27_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7024250.1"
                     /translation="MTFFKKLKKIFLDYVIHIRDRRSPQKNRERVGQNLMILTIFLFF
                     IFIINFVIIVGTDSKFGVNLSKEAKKVYQQSMTVQAKRGTIYDRNGNPIAEDATTYSL
                     YAIISKNYTTATGQKLYVQPSQYEKVASILENKLGMKKNLVLKQLNQKKLFQVSFGSS
                     GSGLSYTKMADIKKTMEKSDIKGIGFSTSPGRIYPNGIFASQFIGFTLPQDDGDGKKL
                     VGNTGLEAALNKVLSGTDGKVTYEKDRSGNVLLGTATTERRAVNGKDIYTTLSEPIQT
                     VLETQMDVFAEKTKGKFASATVVNAKTGEILATSQRPTYNPSTLKGYDKKNLGTYNTL
                     LYDNFFEPGSTMKVMTLASAIDSKHFNSTEVYNSAQYKIADAVIRDWDVNEGLSSGSY
                     MTFPQGFAHSSNVGMVTLEQKMGRDKWLNYLSKFKFGYPTRFGMLHESGGLFPSDNEV
                     TIAMSSFGQGIGVTQVQMLRAFTSISNDGVMLQPQFISSIYDPNTGTSRTARKEVVGK
                     PVSKEAASKTRDYMVTVGTDPYYGTLYAAGAPVIQVGNQSVAVKSGTAQIAQEGGGGY
                     LQGKNDTINSVVAMVPSENPDFIMYVTIQQPEKFSITFWKDVVNPVLEQATAMKETIL
                     KPVLNDSEHQTKYKLSKIVGENPGHVAEELRRNLVQPIILGNGSKVSKVSKRPGANLA
                     ENEQLLVLTNKLTELPDMYGWSKANVEQFAKWTGIKVTYKGSTSGKVRKQSIDVGKSI
                     NKIKKIKITIGD"
     gene            27773..28783
                     /locus_tag="VBD27_000209"
     CDS             27773..28783
                     /locus_tag="VBD27_000209"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO7024251.1"
                     /translation="MFLSIMAGVIAFVLTVIAIPRFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLVVALLVSLIFSIILSKENSGNLGATFGILSVVLIYGIIGFLDDF
                     LKIFKQINEGLTPKQKMSLQLIAGLIFYFVHVLPSGTSAINIFGFNLEVGYLYAFFVL
                     FWVVGFSNAVNLTDGIDGLASISVVISLITYGIIAYNQTQFDILLIIVIMIGALLGFF
                     VFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLFIGFVYVFETSSVMLQVAYFK
                     YTKKKTGVGKRIFRMTPFHHHLELGGVSGKGNKWSEWKVDAFLWAIGIFMSAITLAIL
                     YL"
     gene            28881..30224
                     /locus_tag="VBD27_000210"
     CDS             28881..30224
                     /locus_tag="VBD27_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7024252.1"
                     /translation="MSFKDFNFKPYIQRALDELKFVDPTDVQAKLIPVVRSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDESSDDVQVVITAPSRELATQIYQATKQIAEHSEQEIRV
                     VNYVGGTDKLRQIEKLKVSQPHIVIGTPGRIYDLVKSGDLVIHKAHTFVVDEADMTLD
                     MGFLDTVDKIAGSLPKDVQILVFSATIPQKLQPFLKKYLTNPVMEKIKTATVIADTID
                     NWLLSTKGRDKNAQILELSKLMQPYLAMIFVNTKERADELHSYLSSNGLKVAKIHGGI
                     APRERKRIMNQVKNLEFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGTAITLYQPSDDSDIRELEKLGINFIPKVIKNGEFQDTYDRDRRNNREKSYQK
                     LDTEMIGLVKKKKKKIKPGYKKKIQWKVEEKRRKERRASNRAKGRAERKAKKQSF"
     gene            30362..>31007
                     /locus_tag="VBD27_000211"
     CDS             30362..>31007
                     /locus_tag="VBD27_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7024253.1"
                     /translation="MFKKIILSIATIAATASLAVSVQASEKVELKVATDSDTAPFTYQ
                     KDGKFKGYDVDVVKAVFKGSKYKVTFKTVPFDTISTGIDAGKFDLSANDFSYNKERAE
                     KYLFSDPISRSNYAVVGKKGSHYKSLSDLSGKSTEVLSGVNYAQVLENWNKNHPNKKP
                     IKIKYVSGTTGVTSRLKNIESGKIDFILYDAISSDYIVKDQSLNLSVSPLKGKIG"
BASE COUNT         9984 a         4836 c         6316 g         9871 t
ORIGIN      
        1 ggatataagc tatgataagc gaaagaatta gaaacatata tcgaaagaaa tatttctttt
       61 ctttcgattt atcgaagtta tatgtagcat atattatggt atcagttatc ttgacagcaa
      121 taatactgat aaagattgca aaaagaacat ctaatagagt aatagggatt agatctggta
      181 ctgactgttg aagtgcttca gtaatgagat atcctcctaa aaagataatc atttggatag
      241 ttagtagtaa aatggctaaa tcccaacgtt gtaatgtttc ttctttctta gagattttag
      301 gtgtagattt aataacttgg tttataaagt cgtgcctatc ttctccaaat acactaacta
      361 aacttgcttg tcgctcttgt gcttctaata acatattgac aatatcattt cgaatgagct
      421 cctggtggta ggggctaatg ctagcacctc gcaaatagac aatcatatct gtataggttt
      481 ggttattaga gttatcaatt cttccttcta atagcgtatt ttctttttgt aattttttca
      541 gtttatccat gctagtttcc tctccaattt ttagagtagt ttgtttaatg ctttactaag
      601 tgactcccaa ttctgtttaa attgggctaa ttgctccacg ccttcattgg ttaacgtata
      661 atatttacgt ttaggtcctt gaggggaagg cttttgttgt ccttttatat atttattgct
      721 ttgtagcctt aagataatgg ggtagattgt cccttcggaa atttccccaa aaccataaga
      781 atttaatttt tttgagattt cataagcata agtctcctct tgctgtatga tttttaggat
      841 agctccttca agtaatcctt ttagcatttg cgatggaatc atattatatc cttatctata
      901 ttgtaataca agttactggt taactacatt gtaatacaaa gtagttgaga tgtcaaatat
      961 aactaagtta ccggtagaaa ataagagtga tagacgatga ttcttctaaa ataggtttgt
     1021 ttatgtcctt tagtttatgt tataataatg tgaagaaact ttaggagtga gtatgaaaat
     1081 agataaaaaa gaacttttag cacttattgc tagtattatt ttattaattt ttgcttctgt
     1141 tacgtttttc ttatttaaag atcatggcac tactcaaatg gatactgtag aaagcagtgt
     1201 taaccatgtc agtgattctc aattaactga ggcacaagat atgttagata aatttgagaa
     1261 gaaaccttca gaaaaattat tgaaggatgt cgaacttgct ttaaataaat tgtccaattc
     1321 ttctaaaaaa gaagctttgc aaaaacgttt taagaaagca aaagataagt atcttaaaga
     1381 cgaagcagat aagaaagcta ccaaagatgc tacagattta gtagaaattc tagaacaagc
     1441 cccttcagaa gaaaatgtgt taaaagctga agcagcagtc aaaaagttaa ctgtcaaaga
     1501 atctaaagaa gctttacaaa aacgtattga tactgttaag acacaatatg gtttgattgg
     1561 gaatcaaact ccctctagtt cagtagctga aactacagaa caagggacag ctaatcctgc
     1621 tagtcaggat acttctagtt acgttaatca ggatgtagca ccaacttatg agcaaccgca
     1681 aacgaataat acaccagtta ctccaggggt taacaatact gttccgactc caggaactgg
     1741 tactgtacct gctactaatg ggacgggtgt tgctcagtaa aatcctgaaa gtctagtttt
     1801 actagacttt tttgctatgc tttttaatta gccattctca tcaaaaacta taagagaatt
     1861 agcaattgat agttttggag aaggaaaaga attatgagca atagcaatta aaaccttaaa
     1921 agtacaacca agtgaagtgg tttataacaa gtgtatagtt ctttcttaga ataaaccatg
     1981 actttttaaa aaataggtat ataccattta caaaaatacc ttaaagcgtt ataatgagag
     2041 tgaatggggg gaagaagatg acgaagtaca tcaaggctga ccgattcttt tatgctgatc
     2101 atgtaaagga aaacggttat ttagaaataa aagataatca ctttggtaaa tggatagaaa
     2161 atatttctgg acaagaggag attttagatt attcaggtta ccaaatagca cctggtttag
     2221 ttgatacaca tattcatggt tttgctggcg ctgatgttat ggattgtgat tcagaaggaa
     2281 ttttgcgaat gagtgctggc ttactatcta ctggagtcac atctttcttg ccgacaaccc
     2341 taacttcaga tacaaagcga ctagaagagg cgtctaaagc agttgcggca gtagcgggca
     2401 aagaacaagg agcaaaaatt caaggcatct attttgaggg accatatttt actgaggaat
     2461 ataaaggagc tcaaaatccg atttatatga gaaatccaaa cttggaagag tttgctcaat
     2521 ggcagaaggc tgcaaaaggc ttgattacaa aaattgcttt ggcaccagaa cgtgagggag
     2581 ttgaggaatt tgtctcagca atcacaaagc aaggtgttac ggtagcttta ggtcattcta
     2641 atgggacata taaagaagct aaaaaggcag tgaaggcggg tgcctcagtt tgggtacatg
     2701 cgtataatgg tatgcgtggt ttgacacacc gagaaccagg tatggttggt gcggtttata
     2761 atttacctaa tacttatgct gaactcattt gcgatggtca tcacgttgat cctgttgctt
     2821 gtgacatttt aatgacacaa aaaggccata atcatgtggc attaataaca gattgtatgg
     2881 cagctggagg tgcccctgat ggagattata tgttagggga attgcccgtg gttgtgtcta
     2941 atggaactgc gcgtttgaaa gatggaggga atttagctgg ttctattctt aagctcaaag
     3001 atggattaaa aaatgttgta gaatggggaa ttgctactcc ggaagaggca gtgcgtatgg
     3061 cgagttatgt tccagcagtg tctgtaggca ttgataatgt ctgtggacag ataaaagaag
     3121 gctatgcagc ggattttatt gtattagata aacatctaga tttagttgca acataccttg
     3181 atggagaaaa acgttggcaa tcttgaaaga actagttttt actagttctt tttaatttat
     3241 taatttttaa tgaaatatag tttgacaata aaactatttt tcttataatg ttgtaatata
     3301 atacaaaaca ataggagttt acaatgtcaa ttattattgg gttaatggcc gctatgttta
     3361 tcattcgttt agcttattta aagctatcta tagctaatga aaaggcctta agaaaaaatg
     3421 gtgctaaaga atatggtgta ggtgtttcaa aagcaatcac agttcttcat attatcattt
     3481 atttttcatc tgtaactgag gctgttctaa caaaagcaag ttttaatttt gttagcgtaa
     3541 tcgggcttag cttgatgatt ttttctgtgt ttatgttaca tacagtaaca cgtttacttg
     3601 gtcgtatttg gacagtgaaa ttaatggtag ataaaaatca tcaatttgta gatcactggc
     3661 tatttagggt tgttaagcat cctaactatt tcttaaatat tgctcctgaa cttttaggtg
     3721 taactttatt gtgtcatgct aagtatacag cattatttgt cttgccaatt tatgcttttg
     3781 ttatttattt aagaattcgt gaagaaaact tattattaaa aacgatcatt attccaaatg
     3841 gaatcaaaaa gtctagggta tcttaagaat aagataaata gtagacataa aagtcatttt
     3901 ttgttatact gtctcagtaa gaagaggtgt tcatcagctg tttttttagc gagctcaagg
     3961 tagtgaaagt tgggtagaat aggatgaata ttggcacttt ttagtgagta aaagtacaat
     4021 caaaaatgaa gtaataaatt agggtggaac cgcgtctttg acgcccctgt gctgttgggc
     4081 ataggtggtg taggacgtgg tttttatcat ttgtccgttt taagtcaatt gttgtcacct
     4141 tcccaacagc tgtaataaag tatgaaagaa ggaaaatgat gtctaagaaa ttaacttttc
     4201 aagaaattat tctaacttta caacagtttt ggaatgacca aggctgtatg ttaatgcaag
     4261 cttatgataa tgagaaagga gcagggacaa tgagccctta tactttctta cgtgctatag
     4321 gccctgagcc ttggaatgct gcatacgttg aaccatcacg tcgtccagct gatggccgct
     4381 atggtgaaaa tccaaaccgt ctttaccaac atcatcaatt tcaagtggta atgaagcctt
     4441 cgccgtcaaa tattcaagaa ctttatttga agtctttgga attactaggt atcaatccac
     4501 ttgagcatga tatccgtttt gttgaagata actgggaaaa tccatctaca ggttcagctg
     4561 gattaggttg ggaagtttgg cttgatggaa tggaaataac acaatttact tatttccaac
     4621 aagtaggtgg acttcaaaca ggtcctgtaa cttctgaagt tacttatgga ctagagcgtt
     4681 tagcctctta tattcaagag gtagattctg tttatgatat tgagtgggca ccaggtgtta
     4741 aatatggtga aatctttact cagccagagt atgagcattc aaaatattca tttgagatat
     4801 cagaccaagt aatgttactt gaaaactttg agaaatttga aagagaagca aaacgtgctt
     4861 tagaagaagg acttgttcat ccagcttatg actacgtcct taaatgttca cacaccttta
     4921 atttattaga tgcacgtgga gcagtatctg ttactgagcg tgcaggctat attgcacgta
     4981 ttcggaattt agcacgtgtt gttgctaaaa cctttgttgc cgaacgtaaa aaacttggtt
     5041 tcccactttt ggatgaggaa actcgtatca aattacttgc tgaggaagac taatgaacac
     5101 actcattgtt aatagtcatc ctgatttttc gaatccgtac tcatttacaa ctattctaca
     5161 agaaaagttt attgaacttt ataatgagca tttccctaac catcagttat ctattttaaa
     5221 tttatatgat tgtgttttgc cagaaattac gaaagaagta ctactttcga tttggtctaa
     5281 acaaagaaag ggtttggaat taacagccga tgagattgtt caagctaaaa tctctaaaga
     5341 tttacttgaa cagtttaagt cacatcaccg tattgtcttt gtttctccaa tgcacaatta
     5401 caatgtgact gctagagcaa aaacttatat tgataatatt tttattgcag gtgagacttt
     5461 caagtacact gaaaatggtt cggtcggatt aatgacagat gactatcgtc tgcttatgtt
     5521 agaatctgcg ggttctattt attctaaagg acaatatagt ccatatgaat ttcctgttca
     5581 ttatttaaaa gctatcttta aagattttat ggcttttgat gatttctgtg ttgttcgtgc
     5641 agaaggtacc gatattttag ataggcaagt tgttcttgat aagatgaatc aggatttaag
     5701 agaagcattt gaagcgtttt atagtaagga gtaggaaatg actaaagatt tattattgga
     5761 gcttggactt gaagagttgc cagcttatgt tgtgacaccg agtgagaagc aattgggcca
     5821 aaaaatggtc aagtttttag aggatcatcg tttatcattt gaaactgttc aaactttttc
     5881 aactccacgt cgtttggctg ttcgtgttaa aggattggca gaccagcaga cagatttaac
     5941 agaagatttc aagggtcctt ctaagaaaat tgctttggat gcagaaggga atttttctaa
     6001 agcagcccaa ggttttgttc gcggaaaagg tttaagtgtt gatgatatcg aatttcgtga
     6061 agttaaggga gaagaatatg tttatgtcac taagcatgaa actggaaaaa gtgcgattga
     6121 tgttttagcg agtgtgacag aggtattaac agaattgact ttcccagtga acatgcattg
     6181 ggcaaataat agttttgaat atattcgtcc agttcacact ttagtggtat tattagatga
     6241 tcaagcctta gagctagact tccttgatat tcattcaggg cgtattagtc gaggacaccg
     6301 tttcttgggt tctgacactg aaatttcatc tgccagttcg tatgaagatg acttgcgaca
     6361 acaatttgtt attgctgatg caaaagaacg tcagcaaatg attgttaatc aaattcatgc
     6421 tatcgaagaa aagaaaaata tttcagttga aattgacgaa gatttactaa atgaagtgct
     6481 taatttagtt gagtatccga cagcattctt gggttcattt gacgagaaat accttgatgt
     6541 acctgaagaa gttttagtca cttcaatgaa aaatcatcaa cgctattttg ttgttcgcga
     6601 cagggatgga aaattgttac caaactttat ttctgtgcgt aatggaaatg ctgagcatat
     6661 cgaaaatgtt ataaaaggca atgaaaaagt tttagtggct cgtcttgaag atggtgaatt
     6721 cttctggcaa gaagatcaga aacttaatat tgcagattta gttgagaaat taaaacaagt
     6781 aactttccat gaaaaaattg gttcacttta tgaacatatg gatcgtgtaa aagttatttc
     6841 acaacatttg gctgataagg caggtctttc tgatgaagaa aaattagctg tgcttcgagc
     6901 tgcaagtatt tataaatttg acctcttaac tggaatggtt gatgagtttg atgaattaca
     6961 aggtattatg ggtgagaaat atgccttact tgcaggggaa cagcccgcag tagcagcagc
     7021 gatacgtgaa cattatatgc cgacatcggc cgatggagag cttccagaaa ccagaattgg
     7081 tgcaattcta gcgttagctg ataaatttga caccttactt tcattcttct cagttggttt
     7141 gattccaagt ggttcaaatg atccatatgc tcttcgtcga gctactcagg ggattgtccg
     7201 tattttggaa gcttttggtt gggatatccc acttgatgaa ttagtaacta acctttatgg
     7261 tctatcgttt gctagtttag attatgctaa tcaaaaagag gtcatggcct ttatttcagc
     7321 tcgtattgaa aagatgattg gctctaaggt ccctaaagat attcgagaag ccgttttaga
     7381 atcagacact tatattgtta gccttattct agaagctagc caagcattgg tgcaaaagag
     7441 caaggatgct caatataaag tttccgttga aagtttgtca cgtgcattta atttagctga
     7501 aaaagttact cattctgtat cggtggactc ttcattgttt gaaaataatc aggagaaggc
     7561 gctatatcaa gctattttgt cacttgaact gactgaggat atgcatgaca acttagataa
     7621 gcttttcgca cttagtccaa ttattaatga tttctttgat aatacaatgg taatgacaga
     7681 tgatgaaaaa atgaaacaaa atcgtctggc tttactcaat agccttgttg caaaggcaag
     7741 aacagtcgct gcttttaact tattaaacac aaaataagga ggcattttat ggatcctaaa
     7801 aaaattgctc gtattaacga gctttcaaaa aagaaaaaaa cagttggatt gaccggagaa
     7861 gagaaggttg aacaagcaaa gctccgtgaa gagtatattg aaggtttccg ccgatcagtc
     7921 cgtcaccacg ttgaaggaat taaattagtg gatgatgagg gcaatgatgt taccccggaa
     7981 aaacttcgtc aagtccagag agaaaaagga ctacatggtc gtagtttgga tgatccaaat
     8041 tcttaatggt gagacaattt taaattaaag tcaacacttg ggtggggcaa aagataatct
     8101 ttgtctcgcc ttttttatta ggaaggaaaa aataatgatt gaattactat tagcattact
     8161 gttatggaat attttagtat ttgcaaccta tgctattgat aaaaggcgtg caattagagg
     8221 ggcatggcgt attagtgaga aaacattgtt gatagagagt ttattattag ggggatttgg
     8281 tgcattccta ggtggagaag tttttcgaca taaaacgact aaatggtatt ttagactagt
     8341 ttggtatatc ggaatgatta ttgatttagc tattatttgg ctaatttggt gttattgatg
     8401 atatcttatc aaaaatttta ggggcatatt aaatttttga tagtgcagga tagactgatt
     8461 taggtataat agaattacaa aaagaaaacg cttactttta aggaggaagt ttatgtcttc
     8521 agaagaaaaa tacgttatgg cgattgacca gggaacaaca agttctcgag caattatttt
     8581 taataaaaaa ggtgaaaaaa ttgcgagtag ccaaaaagaa tttccccaaa tttttcctca
     8641 agcaggctgg gtagagcata atgctaatca gatttggaat tctgttcaat ctgtcattgc
     8701 tggagctttt attgaatcaa gcatcaaacc tggacagata gaggctattg gtattaccaa
     8761 tcaacgtgaa acaactgttg tttgggataa aaaaacaggt cttccaattt ataatgctat
     8821 tgtatggcaa tcgcgacaaa cagccccgat tgctgatcaa ttgaagcaag aaggtcatac
     8881 taatatgatt catgaaaaaa ctggtcttgt cattgatgct tacttctcgg caacaaaagt
     8941 tcgttggatt ttagaccatg ttcctggagc acaggaacgt gctgaaaaag gagagttgct
     9001 ttttggaaca attgatactt ggttagtttg gaagttaact gacggtctcg tccatgttac
     9061 agactattca aatgctgctc gtacgatgct ttataatatt aaagaactta aatgggatga
     9121 tgaaatttta gagttactga atattcctaa agcaatgtta ccagaagtaa aatctaattc
     9181 tgaagtttat gggaaaacta ctccattcca tttttatggt ggagaggtgc caatttctgg
     9241 tatggctggt gaccaacagg cagcattatt tggacagttg gcctttgaac ctggtatggt
     9301 aaaaaacact tatggtacag gttccttcat tatcatgaat acgggcgaag agatgcaact
     9361 gtctcaaaat aatctattaa caacgattgg ttacggcatt aatggtaagg ttcattatgc
     9421 acttgaagga tcaatcttca ttgcaggaag cgctattcaa tggttacgtg atggtcttag
     9481 aatgattgaa acgtcaagtg agtcagaagg ccttgctcaa tcatcaacaa gtgacgatga
     9541 ggtttatgtg gttccagctt ttacaggttt gggtgcaccg tattgggatt caaatgctag
     9601 agggtcagta tttggattaa ctcgtgggac aagtaaagaa gattttgtta aagctacact
     9661 tcagtcaatt gcttatcaag tacgagacgt tattgacact atgcaagttg attcaggaat
     9721 tgatatccaa caacttcgtg tagatggtgg agctgcaatg aataatttat taatgcaatt
     9781 ccaagcggat attttaggga ttgatattgc tcgtgcgaag aaccttgaaa ctactgcctt
     9841 gggtgcagct ttcttagccg gactttcagt tggctattgg gaaagtatgg atgaactaaa
     9901 agaactgaac gcaacaggac aacttttcca agcaacaatg aatgagtctc gaaaagaaaa
     9961 actgtataaa gggtggagaa aagctgtcaa agcgacacaa gtatttgctc aagaggatta
    10021 agggggtgta gagatggaat tttcacgaga aacacgacgt ttagccttac aaagaatgca
    10081 agatcgtact ctggatttac ttatcatagg tgggggtatc acaggcgctg gtgtcgcttt
    10141 acaagcagca gctagtggat tagatactgg acttattgaa atgcaagatt ttgcagaagg
    10201 gacaagtagt cgctcaacaa aattggtcca tggtggtctt cgttacttga agcaatttga
    10261 tgtagaagtt gtttctgata cagtatcaga gcgtgcagtc gtgcagcaaa ttgcaccaca
    10321 cattcctaag ccagatccaa tgcttttacc ggtttacgat gagccgggtt ctacttttag
    10381 tatgtttcgc cttaaagtgg ctatggattt atatgattta ttagcaggtg tgacaaacac
    10441 acccgcagcc aataaagttt taagcgcaga agatgttctt aagcgtgagc ctgatttaca
    10501 aaaagaaggt ttgctaggcg gaggggtcta tttagatttc cgtaataacg atgcacgcct
    10561 agtgattgaa aatattaaac gtgctaatag ggatggagct tatattgcca gtcatgtcaa
    10621 agctgaggac ttcttatttg atgacaacaa tcaaattata ggagtacgtg cgcgtgattt
    10681 gttgactgac caagttatcg atataaaagc tagacttgtt attaatacaa ctggaccttg
    10741 gagtgacacc gttcgcaact tctccaatga aggtaaacaa attcatcaat tacgtccaac
    10801 taagggagtt cacttagttg tagatcgcca aaaattaaat gtttctcaac ctgtttatgt
    10861 tgatacaggt ctaaatgatg gtcgcatgat ttttgttttg ccacgtgaag ataagactta
    10921 ttttggaacc acagatactg attaccatgg agacttagaa catcctacgg taactaaaga
    10981 agatgttgac tacctcctta atattgtaaa caaacgcttc ccagaagcag agcttactat
    11041 tgatgatatt gagagttcat gggcaggtct gcgtccacta ctatcaggga atagtgcctc
    11101 tgactataat ggaggcaata gtggaaaact aagcgatgaa agttttgaag aattaattga
    11161 ttccgttaaa gattatattg cacataaaaa tcatcgtgag gatgttgaaa aagctatttc
    11221 tcatgtagaa tctagtactt ctgaaaaaga attggaccca tcagctgttt ctcgaggatc
    11281 aagttttgag cgtgatgata atggcctatt aacacttgct ggtggtaaaa ttactgatta
    11341 tcgaaaaatg gcagaaggtg ccatggaaac tattattaac attttggata aagaatataa
    11401 tcgcaagttt aaactaatca attctaaaac ctatcctgtt tcaggaggag agattaaccc
    11461 atctaatgtt gattctgaaa ttgaagctta tgctcaattg ggcacactaa gtggtttaag
    11521 tattgaggat gcacgttata ttgctaacct ttatgggtca aatgcaccaa aattatttgc
    11581 attgacacgc caaattacag aagcagaagg tctgagcttg gtagaaacgt tgtcacttca
    11641 ctatgctatg gattatgaaa tggcgcttag cccaacggat ttcttcttaa gacgtacgaa
    11701 tcatatgctt ttcatgagag ataatttaga ttctttgatt cagcctgtta ttgatgagat
    11761 ggctaaacat tatcaatggt ctgatcaaga caaaactttc tatgaagaag aattacatga
    11821 gactctaaaa gacaatgact tggcagcttt gaaggataat tgaaggagat aataatggat
    11881 atttttggag aattcttagg aacagctctc ttagtcttac ttggtaatgg tgttgtagca
    11941 ggtgttgttt tgcctaaaac gaagaatcat aattctggat ggattgttat cacatttgga
    12001 tgggggcttg ctgtagctat tgcagcgttg gtatctggca atattagccc tgcccacttg
    12061 aatccagctg tgtcattagc ttttgcgatc aaaggtgatt tagcttgggg aacagctatt
    12121 ctttatatga ttgcacagat tattggtgca atgctaggtt cacttcttgt ttatttgcaa
    12181 tttagacccc attatgaggc tgcagaaaat aaagcagata tccttggaac ttttgcaact
    12241 ggtccggctt tgaaggataa tttttccaat tttttaagtg aagtattggg aacacttgtt
    12301 cttgtactta ctatttttgc catcggaaaa tacaatatgc ctccaggagt tggtacgatg
    12361 agtgttggta tgttagtggt tgggattggt ctatcgctcg gtggaacaac aggttatgct
    12421 attaatcctg cacgtgattt tggtcctcgt ttattgcatg ccttgttacc gatgaagaac
    12481 aaaggtgatt ctgattggac ttattcatgg attccgatag taggacctat ggtaggagct
    12541 attttagcag ccttaatatt tgctatgatg taagagattt ttgatgctga gaccgacttt
    12601 atggtctcag ttttttgtta ccgttttcaa aaataacttc ttttgttata ataagtaatt
    12661 atgacagaaa aatatgtaaa taaagtaatt gtcattttag gagcttcttt tgctggtatg
    12721 acttgtgcgc agaaattacg ccaattaaat cctaattggg atatcgtttt gattgataaa
    12781 gaaattcatc cagattatgt tccaaatggc ttaaattggt attatcgtca cgaaattagt
    12841 ggtttaaatc aggccatgtg gcagacagaa gaagagcaac ggttgcagaa tatacgctgt
    12901 ctattatgat caattgattt gtgccatggg ctcccaagca gaatcaactt atattgatgg
    12961 ggctgatgcg caaggtgttt taacaacaaa aacttatgca actagtcaaa atgctaagca
    13021 ggtattagat aaaagtcata aggtagcagt tgttggggca ggtatcatcg gtttagatat
    13081 tgcttatagt ttacatgaat cagggaaggc ggtcactttg ttagaagctc aagaaagacc
    13141 agacttcagg catacagatc ctgatatgtc ccttccttta cttgatgcga tggctgaatc
    13201 aaaactacat tttttccaaa atcaaaaggt tgagaaaatt actgtaaccc gagaagaaaa
    13261 actttgtttg cgaacgctaa caggagacac ctttacagtt gatactgtca tattagctgt
    13321 taattttcgt ccggatagtc gtcttttaac tgggttggtt gatttaagtg ttgataatag
    13381 tgttgttgta aatgattatt tccaaacgag tgaccctaat atctatgcta ttggcgattt
    13441 gatttggtcg tattttaaag gtcttaatag tgcttattat atgcccttaa ttaaccaagc
    13501 aatccgttcg gcacaaatgt tggcatacca tttatcggga catgctgttc caaaattaaa
    13561 aataacacgc gctacaggat ctaaacactt tggctattat agagctaaca ttggattaac
    13621 tgagctggaa gctggtcttt acgaggatac cgttagtgtt acttatttcc ctaaagaaca
    13681 gtatgattta aggataaaat tgattgctaa tcaaaaaacg ggccatttat tgggggctca
    13741 attaatctca aaggaaaatt gtttggctac tgccaatcag cttgttcaag ctatatcgtg
    13801 tgacatgaca gattttgatt tggcttttca agatttcatt tatacagcta gagaatctga
    13861 gatggcttat atgttacatc aagcagctat caacctttat gaaaagagga ttgggctatg
    13921 cgaatagaag ctctaatgga aaaagaaagg cgtgttcaat atcggctact ttctttttta
    13981 agaggctctc ctcaggcaat agcactgaag ttagctttat tagaaactgg tttaagtcga
    14041 gcaactttgc ttaaatatat caataatctt aatagctact ttgaacaaga aaaagttaat
    14101 tgtcgaatcg tttattataa ggataagtta tttttagaag aggattataa cttatccaat
    14161 caagaagtgt tgaaagctct gatgaaagat agtattaaat acactatctt aatatcttta
    14221 tttaatcagc gtcagttcac aatcgttggt ctttctcaag aattgatggt tagtgaggct
    14281 actttaaatc gtcatcttgc tcatctcaat gaattgttag cggaatttga tattgctatt
    14341 tcacaaggaa aacagattgg tgatgaattg caatggcgtt atttttatta tgaattattc
    14401 aagcaactat ggtcttatga caagtgccaa aatatgataa agaaattaga cttagactca
    14461 ttaattctat taatagagcg cttggctcaa catactttaa ctcgagaagc tcagcaaaat
    14521 ctggggcttt ggtttagtat ttgtcatcat cgtctccttg ctatggaaaa actatctgat
    14581 aatctcaagc ccattgtgga acattatcaa tgtaatgctt tttataaacg gttagacgca
    14641 gccttagttt tgtatatgag tcgttttgct cttgagtata gggaaggaga agttctggca
    14701 acttttgctt ttctacattc acaaaatatt ttaccaatca atacaatgga atacatcatg
    14761 ggatttggag gcccgattat tgattgtgta accgaaacaa tcatctactt taaaaaggag
    14821 agtattttag ctgacgagac atcagatcag gttatttacc aacttggtca gttatatagc
    14881 cattattatt tctttaaagg tcatatttta gttgaacaac ctgatttaga acaaacgtat
    14941 cgacttattg atcataatat gagggataag ttgcatgata tttcaaagaa gattattgca
    15001 aatgttaatc gaattagacc tttaacagaa gatggttgtt ctttgctgac tttacatttg
    15061 ttggaattgc ttattttctc gaaaaatagt caaaaaatgc cctttaggat tggcctagat
    15121 atgacaggta atgctgtgga acagtcattg ttggaatatc gcattcgaca acatttctct
    15181 ggtaataatt ctattcaagt cgaaccttat gacgaaggaa agggatttga tatggttatt
    15241 tatcagagtc gctcaagacc ttataaagcc aaactaacct attgccttaa taaaggtgct
    15301 tcagagcgag aactacagga aatagattca cttatttatg acaattaatc ccattactta
    15361 attgaatggt tttaattcct ccagaaattg ttaaaacgtt tacgtatttt atgttataat
    15421 atcaaagacg aacaatttta aatttagttg ttaaaaacag aaaggtttta tcatgacatt
    15481 tgacaccatt gatcaattag cggttaatac agtccgcacg ctttctattg atgctatcca
    15541 agcagcaaat tctgggcacc caggtcttcc tatgggagct gcgcctatgg cttatgtgct
    15601 ttggaataaa ttcttaaatg taaacccaaa aacaagtcgc aattggacaa accgtgaccg
    15661 ttttgtactt tcagctgggc atggttcagc tcttctttat agcctacttc atttagctgg
    15721 ctatgattta tcaattgatg atttaaaaca attccgccaa tggggatcaa aaacacctgg
    15781 acatccagaa gttaaccata ctgatggtgt agaagcaaca actggacctt tagggcaagg
    15841 tattgctaat gctgttggta tggcaatggc tgaagctcat ttggctgcta aatttaataa
    15901 accaggcttt gatttagttg atcattacac ttatacactt catggtgatg gttgtttgat
    15961 ggagggtgtt agccaagaag ctgccagtct tgctggacac ttaaaacttg gtaaattggt
    16021 ccttctttat gattcaaatg atatctcact tgatggtcca acgtctcaat catttacaga
    16081 agatgttaaa gggcgttttg aatcatatgg ttggcaacat atcttagtta aagatggtaa
    16141 tgatttagaa gcgattgctg cagcaattga agctgctaaa gctgaaactg ataaaccaac
    16201 cattattgaa gttaaaacta ttattggatt tggcgcagag aaacagggaa catcttcagt
    16261 tcatggagcg ccacttggtg cagaaggtat tacttttgcc aagaaggctt atggatggga
    16321 atatccagac tttactgttc ctgctgaagt tgcagctcga tttgcctcag atcttcaagc
    16381 gcgtggtgcg aaggcagaag aggcatggaa tgatcttttt gctaaatatg aagttgagta
    16441 tccagaatta gcagctgaat acaaagaagc ttttgctggc caagctgaga cagttgaatt
    16501 gaaagctcat gacctaggtt catcagtggc tagtcgtgtt tcaagccaac aagctattca
    16561 acaattatcg actcaattac cgaacctctg gggagggtca gcagacctct cagcttctaa
    16621 taacacaatg gtagcagcag aaacagattt ccaagcaagt aactacgctg ggcgtaacat
    16681 ttggtttggt gtacgtgaat ttgcaatggc tgctgcaatg aatggaattg cgcttcatgg
    16741 tgggactcgt gtttatgggg gtacattctt tgtcttctca aactacttgt taccagcagt
    16801 acgtatggct gcacttcaaa acttgccaac tgtctatgtt atgacacatg attctattgc
    16861 tgttggagaa gatgggccaa ctcatgagcc aatagaacaa ttagcatcag tgcgctcaat
    16921 gcctaatttg aacgttatcc gtcccgcaga cggtaatgaa acaaacgctg catggcaacg
    16981 agctgtatca gagacagatc gaccaacgat gcttgtttta acacgtcaaa acttaccagt
    17041 tcttgaagga acatcagaat tagcccaaga aggggttaat aaaggtgctt atattttatc
    17101 agaagctaaa ggtgaacttg atggtatcat cattgcaaca ggttcagaag ttaaattagc
    17161 tttagatact caagacaaac ttgagtcaga gggcatccat gttcgcgtcg tatctatgcc
    17221 agcacaaaac atttttgatg aacaagaggc ttcatatcaa gagcaagtgc ttccatcagc
    17281 tgttacaaaa cgtcttgcta ttgaggcagg atcaagcttc ggttggggta aatatgttgg
    17341 tttaaatggg ctaacactta ctattgatac ttggggagca tcagcaccag gtaacagaat
    17401 tttcgaagag tatggcttta cagttgagaa tgctgtatcg ctatataaag aactttaaga
    17461 gcatgaagaa gttgggaaaa atacctcaac ttctttttgt aagatatagg atggatttga
    17521 gaatagtttt gatagactga taagtgatgg aaagcactct tttttgaaaa cgtttgacaa
    17581 acattccata gcgcagtata attgataaaa ctattgtata tataaaataa tacaactatt
    17641 attaattatc tttttagaaa ggtttgttta tgaagaagga gtgtcgtgat ttttatcggc
    17701 agatacagca tacttacaat gatatatctg tccgtgaaga tgcagtgcta tcatcaatac
    17761 tattatcagc atcgaatggt ttgataaaaa ctagcgatgt acctcgtgtt gcttatgagt
    17821 taactcagca attggaaaat aatgagatag aaaaatcttt cgagtcatta gctacagtta
    17881 aggaattaaa aaaatctgct aaaaaattgt ctgagagata tgagcaaaat aaggtaacaa
    17941 cgggattgct ttctaaagta tttggaaatc attaggagat aaaaggagat ttttatgatt
    18001 aagtttgaac atgtttcgaa agtatacggg gaaaaagaag ctctcagtga tttaaccctc
    18061 tctattaaag atggtgagat ttttggatta atcggtcaca atggagcagg aaaaacaacg
    18121 acaattagta ttctaacatc tattattgat gcaacttatg gccaagtcta tattgatgac
    18181 ctacttttga cagaacatcg tgaccaaatt aagaaaaaaa taggttatgt accggactcg
    18241 ccagatattt tcttgaattt aacagcagag gaatactggt actttttagc taaaatttat
    18301 gatgttgccc cagaagatat tgaagcacgt atcacaaaat tggtagacat atttgaacta
    18361 gaggagcagc gctataatcc tattgaaagt ttttcacatg gtatgcgtca aaaagtcatt
    18421 gttataggtg cccttcttcc aaatcctgac atttggattc tagatgagcc attaactggt
    18481 ttagatcctc aagcttcttt tgatttgaaa gaaatgatga aagaacatgc taaaaatgga
    18541 aaaacagtta ttttttcaac acatgtctta gcagttgcag aacagttatg cgacagaata
    18601 ggtattttga aacaagggaa attaatcttt gtcggtagct taggtgagtt aaaaatgcaa
    18661 tatccagata aagatttgga aactatctat ttggaattag caggacgtca agctaatagg
    18721 gagggataaa gtatgaattg gtcacgtatc tgggaactcg taaaaattaa tatcctttat
    18781 tcaaaccctc agactctatc ggcactaaga aaaaagcaag aaaagcatcc taaaaaagaa
    18841 ttttcagctt ataaatccat gtttagaaat cagttatttc agattttgct cttttcaata
    18901 atttatgtat ttctctttgt atcacttgat tttaaagaat atccgggcta tttcacgttc
    18961 tacattggta tctttacact agtatccatt atctactctt ttattgcgat gtacagtgtt
    19021 ttctatgaga gtgacgatgt taaacaatat gcttatttgc ctattaaatc tgaagagctt
    19081 tatgttgcta aaatttttgc aacatttggg atgtctgtaa cttttctaat gccaatccta
    19141 accttaatga ttgtggctta ttggcgtata ataggtggcc cacttgcggt cctcctagca
    19201 atcataaatt ttgctatttt atttttatcg gtaacggtga tttcattgta tattaatagt
    19261 ttaataggcc gtgccattat tcgaagtgct aatcggaaac ttatttctac tatattaatt
    19321 tcactagcta catttggagc gatagttccc ttattatttg tgaatatgac cagtcaaaag
    19381 atggtacaag ggaaattaca agatatcgct ccaattcctt atgtcagagg ttattatgat
    19441 attgttacag cgccgtttag tatggagagt cttctcaact attatctccc gctcttaatc
    19501 attttattct tgataggtgc tatttataaa tgggttatgc cacgctacta tcaggagttg
    19561 ttgtatggac aggtcaagca gagaaaagtc catcgtcaaa tcgattttag taaacgtgag
    19621 tcaattaata aaactcttgt taaacaccat ttatcaagtt tacaaaatgc gacattattg
    19681 acaaatacat ttttaatgcc actattatat ctagcaatgt tcattgttcc aattttgaat
    19741 aatggtaaag agattggtcg attttttaat gaaaattatt ttgggatagc ttttctagca
    19801 ggaatattaa taggtagttt atgtgtgatg ccagctagca ttgtaggggt aggtatttct
    19861 ttagaaaaaa gtaatttcta ctttataaaa agtttgccta tatcattttc gtatttctta
    19921 aagcataaat ttgtaacttt gataacatta caacttgcag ttcctacttt tatttatttc
    19981 ttggtaggat tctttctact taaactatca atcctcgttt tattgagctt cattttaggt
    20041 ctagtcttca tgggacttat cgaaggccag tttatttata gacgtgacta taaacatttg
    20101 ttcttgaatt ggcaagaggt tacgcaacta tttaatagag ggttaggcca atggctgtta
    20161 gttggttcat tgtttggaat gatgataata ggtagttttc ttattggaat aagtattttt
    20221 tggtcaatgg tatggaatac tgtagctgtc aatatcataa tccttatcat tgggttgctc
    20281 atattatcaa tttgccaata cttactgcta aaaaattttt ggaagaagct tgtttagtaa
    20341 aaagatttta gaaaaatcat acagtaagaa taatgcttaa taactgagat agttatagcg
    20401 gataaaataa aggcttgttg tggactttac ggttggaagg cagccactgt aatcctttgt
    20461 ttaataacgc tagttttagc tctaaaatat ttgattttta ttgctatcta ttatatctta
    20521 tcagacaaag agactaggga taacctagtc tctttgtctg atgctaactt gtttttcgta
    20581 atattgtgct agaatgaatg tcaatatagc agttgtgctt acagtaatga taactgcaat
    20641 aagtatggta ataaaaagat gtgttgaagt atgactgata taaagaaata gacctggtag
    20701 accagaagag cctacagcga agcggtgagc gttcattata ccggcataga gtcctccaag
    20761 accaccacca ataagtgagg ctattagagg ataacgtttc ggaaggttga caccatataa
    20821 gataggttcg gtgattccca tataagctgt tatggcagca ggtccagcaa tttctttaag
    20881 ctttttttct tttgttataa gagtcaccac tgtacctgca gttgcttgtg cgatatttga
    20941 gcagagcatg cctggtccaa aaatagaatc aacccctaat ttcgctagtt gtgtgattcc
    21001 taatgcagca attccactat gaacaccaaa catgattagg ataggagcaa atgctcctac
    21061 tagaaaagca ggtgcccaac tggcgtattt ggataaaaaa agtaaaaata acagctaaat
    21121 attggccagc gatagtaccc attggtccca atatagagaa cgacaaaatt cccatgctga
    21181 gaaatattaa cataggaaca agcactaaac gtagtgattt aggaataatt tgatttaaat
    21241 atttttctat ataagcctga acacaaataa ttagaataat gggaatgaca cttgatgcat
    21301 agttagtaag tgtgaatggg atcgtatgga ataaggatac tggtttacca gaagctacta
    21361 aattagccca attagggtga aggagcatga caacagttcc caaagctaaa ataggattag
    21421 ccttcaattt ttgagcagct gtgatggcga ttaagatagg taaaaaatag aaaacaccat
    21481 ctgcaaaaag atttagtaat atatatgttt gagataacgg tgtcaagatt ttgaatacta
    21541 gtaataaagc tagcaatgcc ttaatcatac cagcgcctga tagagctggt aaaatcggtt
    21601 ggaaagtccc agaaacaaaa tcaataattc gactaactgc tttggctttt ttggtttgtt
    21661 ctgttttagt cgttgagtta gtttcaatga gggagttaat ttcttcaaac acttttgcga
    21721 catgagtccc gatcactatc tgatgttgcc ctccgctgat aacgacagtt tttacctctt
    21781 ttaaagtttt taattgctca aggtttactt tcgtcggatc attgagacta aagcgaaggc
    21841 gtgtttgaca atggtaaaca ttgttaatat tattttgccc accaaggtgt ttgatgatat
    21901 tttttgacaa ggtgatgtat tctctagaca taatatctcc ttaatactta taaaacgttt
    21961 tccgattcta gattatagca tagtaataaa agaaaacact tggaaatgct tgtgaatttt
    22021 caatgatgtt ttcagatagc attcctaaag ggtctatgat ataatttttt tatgaaaaga
    22081 cattttgaga caactagaag aattgttatt aaagtaggaa ccagttccct cgtacagaca
    22141 agtgggaaaa ttaatttaag taaaatagac catttagcat ttgttatttc tagtcttatg
    22201 aataaaggta tggaggtgat tttagtatca tctggagcta tggggtttgg tttagacatt
    22261 ttgaaaatgg ataagcgtcc tcaagaaata tcacaacaac aagcagtttc tagcgtagga
    22321 caagtagcca tgatgagtct ttattctcag atattttcgc actatcaaac tcatgtttcc
    22381 caaattttat taacacgtga cgttgtggtt tttccagaga gtttgcaaaa tgtaacaaac
    22441 tcttttgaga gtctgctctc tatgggaatt ttgccaatcg tcaatgagaa tgatgctgtc
    22501 agcgtggatg agatggatca taaaacgaag tttggggaca atgaccgtct ctctgctgtc
    22561 gttgctaaaa ttactaaagc agatttatta attatgctgt cagatattga tggtcttttt
    22621 gataaaaatc ctaatattta tgatgatgct gttttacgta gtcatgtttc tgagatcacc
    22681 gatgacatca ttaagtcagc tggaggagca ggaagtaagt ttggtactgg tggcatgctt
    22741 agtaaaatta aaagtgcaca aatggtgttt gataacaatg gacaaatgat attgatgaat
    22801 ggcgccaatc ctcgtgatat tttaaaggtg ttagatgggc ataatattgg aacttatttt
    22861 gctcaaggaa agtgagattg agttatgacg tatattgaaa tattagggca aaatgctaaa
    22921 aaagctagtc aatcagtagc acgattaagt acagctagta aaaatgagat actcagggac
    22981 ttagcgagaa acatagtggc tgatactgaa accattttga ctgagaatgc tcgcgatgtt
    23041 gctaaggcaa aagataatgg tatctcagaa attatggtgg accgtctacg actaaacaaa
    23101 gaccgcatac aggcgatagc aaacggtatt tatcaggtag cagatttggc agatccgatt
    23161 ggtcaagttg ttagtggtta tacaaattta gatggtttaa aaatccttaa aaaaagagtg
    23221 cctttagggg ttattgcaat gatatttgaa agtcgtccca atgtatcggt ggatgccttt
    23281 agtttagctt ttaaaacagg gaacgctatt attttacgtg gtggtaaaga tgctattttt
    23341 tcaaatactg ccttggttaa ttgtatgcgc caaacattgc aagatacagg ccacaaccca
    23401 gatatagtac aattagttga agatactagt catgttgtag cagaggagtt aatgcaggca
    23461 acagactacg tggacgtctt aattccaaga ggtggtgcaa aattaattca aacggtgaag
    23521 gaaaagtcaa agattcctgt tatcgaaaca ggtgttggaa atgttcatat ctatattgat
    23581 gaatttgcag atttagatat ggcagctaag attgttatta atgctaaaac ccaacgccct
    23641 agtgtctgta atgctgctga agggttagtt gttcatcaag ctattgctaa gggattttta
    23701 tctcaattgg agaaaatgct caaagaatcg aaccagtcag ttgaatttcg tgccgatgag
    23761 gaagctttac aacttttaga aaatgctgtt gctgcaagtg agactgatta tgctactgaa
    23821 tttttagact atattatgtc agtaaaagta gtagattctt ttgaacaagc aattagctgg
    23881 attaataaat acagtagtca tcactcagaa gctatcatta ctaataatat cagtagggca
    23941 gaaatttttc aggatatggt agatgcagcg gcagtttatg ttaatgcttc aacacgtttt
    24001 acagatggtt tcgtttttgg actcggagca gagattggta tttcgactca aaaattacac
    24061 gcgcgtggac cgatgggatt agaagcttta acaagtacaa aatactatat taatggaact
    24121 ggtcaagtaa gggaatagtt gacctataat aggagagatt gcatttatcg gtctctcttt
    24181 tacttttcta aagtttagga tttatggtac aattaaagtt atgacaaacg attttcatca
    24241 tattactgtg cttttgcacg agactgtgga tatgcttgat attaagccgg atggtaccta
    24301 tgtagatgct actttaggtg gggcaggaca cagcgaatat cttctttcac aattaggccc
    24361 agatgggcac ctttacgcat ttgaccaaga ccagaaggct attgacattg ctcatatacg
    24421 tttaaagaaa tatgtggata ccggtcaagt aacctttatc aaagataatt tcagaaatct
    24481 atcttctaac ttaaaagcat taggagtgtc ggaaattaat ggtatttgtt atgatttagg
    24541 tgtatcaagc ccacagctgg acgagcgtga acgcggtttc tcatataaac aagatgcccc
    24601 ccttgatatg cgaatgaata gagagcagag ccttacagct tatgacgttg tgaacactta
    24661 tagttatcat gatttagtaa ggattttctt taagtatggc gaagataaat tttcaaaaca
    24721 aattgctcga aaaattgagc aagtacgagc tgaaaaacca atatctacaa caacagaatt
    24781 ggctgagatt atcaaatcgt ctaaatctgc caaagaactg aagaaaaaag gtcacccagc
    24841 taaacaaatt tttcaagcta ttcgtattga agtcaatgac gaactaggag ctgcagatga
    24901 atctatacaa caagcaatgg acttattagc tgttgatggt cgtatatctg tcatcacttt
    24961 tcattcttta gaagatcgtc ttactaagca actttttaaa gaagcatcaa cagtagaagt
    25021 acctaaaggt cttcctttta taccagatga tttacaacct aagatggagc tcgttaatcg
    25081 taaacccatc ttaccaagtc aagaagaact agaagcgaac aatcgtgccc attcagcgaa
    25141 attgagagta gcgcgtcgta ttcgataata agaaagaaac ttatgacaaa tgaaaaacga
    25201 actgaagcag taacacaaac cttacaacgt cacatcaaga ctttctctcg cattgagaaa
    25261 gctttttatg gcgctattgt aattactgct attatcatgg cagtcggtat tatttacttg
    25321 caaagtaata gtttacaagt taaacaagaa gtaaatcaat taaatagtaa aatcaacgat
    25381 aaacagacag aatttgacaa tgctaaacaa gaagtaaatg agctttctaa tcgtgatcga
    25441 attactaaaa ttgctaaaga tgctggttta acaattcaaa acgacaatat ttatcggaag
    25501 gtggactagt ctgtgacttt ttttaaaaag ctaaaaaaaa tcttccttga ttatgtgatc
    25561 catataagag ataggcgttc gccgcagaaa aatagggaaa gagtaggtca aaatctgatg
    25621 attttaacca ttttcctatt tttcattttt atcatcaact ttgtcatcat cgttggtaca
    25681 gatagtaaat ttggggttaa tttatccaag gaagccaaga aagtctatca acaaagtatg
    25741 acagtacagg cgaagcgtgg gacaatttac gaccgtaatg gaaatcctat cgctgaggat
    25801 gctacgacct atagtctcta cgctattatc tcaaagaact atacgacagc tacaggtcaa
    25861 aaactatatg tacaaccttc tcagtatgaa aaagtagcta gtatcttaga gaataaattg
    25921 ggcatgaaga aaaatcttgt cctaaaacaa ctcaatcaaa agaaattgtt tcaagtttcc
    25981 tttggaagtt caggttcagg attgtcttat accaagatgg ctgacatcaa gaaaacgatg
    26041 gaaaagtcag atatcaaagg aattggtttt tctaccagtc cggggcgtat ttatccaaat
    26101 gggatatttg cttcacaatt tataggattt actcttcctc aggatgatgg agatggaaaa
    26161 aaattagttg gtaatacagg ccttgaagcg gcacttaaca aggtattatc aggtactgat
    26221 ggcaaagtaa catacgaaaa agaccgtagt gggaatgttc ttttaggtac tgcaacaaca
    26281 gaacgtaggg cggttaatgg taaggatatc tacacaactt tatcagagcc tattcagact
    26341 gttttagaga ctcagatgga tgttttcgca gaaaaaacca aaggaaaatt tgcgagtgct
    26401 acagttgtca acgctaaaac gggagaaatt ctagcaacga gtcagcgtcc tacttataat
    26461 ccttctaccc ttaaaggtta cgataagaaa aatttgggta cctataacac actactatat
    26521 gataattttt ttgaacctgg ttcaacaatg aaggtaatga ctctagcctc tgcaattgat
    26581 tctaagcatt ttaactctac tgaagtgtat aacagtgcac aatataaaat tgcagatgcg
    26641 gttattcgag attgggatgt caatgaaggg ttatcatctg gtagttatat gacttttcca
    26701 caagggtttg cacattccag taacgttggt atggtaacct tggaacaaaa aatgggacga
    26761 gataaatggt taaactatct ttctaagttt aaatttggtt acccaacacg ttttgggatg
    26821 cttcatgaaa gtggaggttt gttcccatct gacaatgaag taactatcgc tatgagctca
    26881 tttggtcaag gtattggtgt aacgcaagtt caaatgttgc gcgcatttac gtctatctcg
    26941 aatgatggtg ttatgttaca accacagttt attagcagta tttatgatcc aaatacaggg
    27001 acttctcgta cagcacgaaa agaagtcgtt ggaaaaccag tttccaaaga agcagctagt
    27061 aaaacacgtg attatatggt tactgttggg acagatcctt attatggtac attatacgca
    27121 gcaggtgccc cagttattca ggtggggaat caatcagttg cagtaaaatc tggtactgcc
    27181 caaatcgccc aggaaggtgg tggtggttat ctgcaaggaa aaaatgatac tattaactca
    27241 gttgtggcta tggtgccatc ggaaaatcct gacttcatca tgtatgttac catccaacaa
    27301 cccgaaaaat ttagtatcac attctggaaa gatgttgtta atcctgtttt agaacaagca
    27361 acagcaatga aagaaacgat attaaaaccg gtattgaatg actcagaaca tcagacaaaa
    27421 tataaactat caaaaatagt tggtgaaaat ccaggtcatg tagcggaaga attacgtcgt
    27481 aatctggttc agccaattat tttaggaaat ggtagtaaag tttctaaagt atctaaaaga
    27541 ccaggagcta atcttgctga gaatgaacag ctactagtat taacaaataa actgactgaa
    27601 ttacctgata tgtatggttg gtctaaagca aacgttgaac agtttgctaa atggacaggt
    27661 ataaaagtga cctataaagg ttcaacctca ggtaaagtaa gaaaacaaag tattgatgtt
    27721 ggaaaatcca tcaataaaat taaaaaaatc aaaattacaa taggagatta acatgttttt
    27781 aagtataatg gcaggtgtca tagcatttgt cctgacagtt attgccattc cacgcttcat
    27841 taagttttac caattgaaga aaattggcgg gcaacaaatg catgaagatg tcaaacaaca
    27901 tctagccaaa gcaggtacgc cgacaatggg aggaacggta tttttggttg ttgctcttct
    27961 tgtctcactt atttttagta ttattttatc taaagaaaat agtggtaatt taggtgcaac
    28021 ttttggtatt ttatctgttg ttctcatcta tggaattatt ggttttttag atgatttttt
    28081 gaaaattttt aaacaaatca atgagggctt gactccaaag caaaaaatgt cgctccaact
    28141 aattgcgggt ctaatttttt attttgtcca tgtgttacca agtggcacat ctgcgataaa
    28201 tatttttggt tttaaccttg aagtaggata cttatacgct ttttttgttc ttttctgggt
    28261 tgttggtttt tcaaatgctg tgaacctaac agatggaatt gatggtctag cttctatttc
    28321 ggttgttatc agtttgatta cttatggaat cattgcttat aatcaaacgc aatttgatat
    28381 tttattgatt atcgttatca tgattggtgc tttacttggc ttttttgtct ttaatcataa
    28441 accagctaaa gtctttatgg gagatgttgg ttcacttgca ttgggtgcta tgttagcagc
    28501 catttcaatt gccttacgtc aggaatggac cttgcttttt atcggctttg tctatgtttt
    28561 tgagacgtct tcagtgatgt tgcaagtagc atactttaag tatactaaga agaaaactgg
    28621 tgttgggaaa cgtatcttcc gcatgacacc atttcatcac catttagaat taggtggtgt
    28681 gtctggcaaa ggtaataaat ggagcgaatg gaaagtagat gcttttttat gggctattgg
    28741 tattttcatg agcgctatta cacttgctat tttatatctt taaactttat taggggatgt
    28801 ggtatggact gccagattcc cttttgcttt cttgtagcct ttaatggtat aatgaacata
    28861 ataatagtaa ggaaatagac atgtcattta aagattttaa ttttaaacca tacattcaga
    28921 gggctctcga tgagttaaaa ttcgtcgatc caactgatgt tcaagctaaa ttgattccag
    28981 ttgttcgttc aggccgcgat cttgtggggg aatcgaaaac tggttctggt aaaacccaca
    29041 cctttttact tcctattttt gagaagctag atgaaagtag tgatgatgtt caagttgtta
    29101 ttactgcacc aagtagagag ttggcaacac aaatttatca ggcaactaaa caaattgctg
    29161 agcattcaga gcaagaaata cgggtggtta actatgttgg tggtactgac aaattacgtc
    29221 aaattgaaaa gttgaaagta agccaacctc atattgtcat tggaacccca ggacgtattt
    29281 atgatttggt gaagtcaggt gatttagtta ttcataaggc gcatactttt gtggttgatg
    29341 aagcagatat gactctggat atgggattcc ttgataccgt tgataagatt gcagggtctt
    29401 tacccaagga tgttcaaatt ttagtctttt cagcaacgat tccacaaaaa ttacagccat
    29461 ttttaaagaa atatttgacc aatccagtaa tggaaaaaat caaaacagca acagttatcg
    29521 ctgataccat cgataactgg cttttgtcca ctaaaggacg tgataaaaat gcacaaattt
    29581 tggaactctc taaactaatg cagccttacc ttgcaatgat atttgttaat acgaaagagc
    29641 gtgcagacga attacactcg tatctttcta gtaatggctt aaaagtagct aaaattcatg
    29701 gtgggattgc acctcgtgaa cgtaaacgta ttatgaatca ggttaaaaac ctcgaatttg
    29761 aatatatcgt tgcaacagat ttagcagcgc gtggtattga tattgaaggt gttagccatg
    29821 ttatcaatga tgccatccct caagacctat ctttctttgt ccaccgtgtc ggtcgcacag
    29881 gccgtaatgg cttatctggt actgctataa ctttatatca gccaagtgac gattctgata
    29941 ttcgtgaact tgaaaaacta ggtattaatt ttattcctaa agtcattaaa aacggtgaat
    30001 ttcaagatac atacgaccgt gatcgtcgta ataaccgtga aaagtcctat caaaaattag
    30061 atacagaaat gattggactt gtgaaaaaga aaaagaaaaa aataaaacca ggttacaaga
    30121 aaaaaattca atggaaagtt gaagaaaagc gtcgcaaaga gcgtagagca agtaatcgtg
    30181 ctaaggggcg tgcggagcgc aaagctaaaa aacaaagttt ttaaaattat taaagtctga
    30241 aatcagttcg tttcaggctt ttttattgat ataaaatctt tatcacctga taaggaaata
    30301 ctattataca gatagatttt agagtgttag actagtaata tgatatttta ggagaattct
    30361 tatgtttaaa aaaataatat tatctattgc aactatagcc gcaaccgcca gcctagcagt
    30421 atcagttcag gcgtcagaga aagtagaact taaagtagct acagattctg acacggcacc
    30481 atttacttat caaaaagacg ggaaattcaa aggttatgac gttgatgttg tcaaagctgt
    30541 ttttaaaggt agtaagtaca aagtaacctt caagacagtt ccttttgata ctatttcaac
    30601 aggtattgat gcagggaaat ttgatttatc agctaatgat ttttcatata ataaagaaag
    30661 agcagaaaaa tatctcttct cagatcctat atcccgttca aattatgccg tagtagggaa
    30721 gaaggggagc cattacaaat cattaagtga cctctctgga aaatcaacag aagttttatc
    30781 tggcgttaac tatgcacagg ttctagaaaa ttggaataaa aatcatccta ataaaaaacc
    30841 aataaaaatc aaatatgttt ctgggacaac tggtgttact agcagattaa aaaatattga
    30901 gagtggaaaa attgacttta tcctatatga tgccatttca tctgactata ttgtaaaaga
    30961 tcaatcatta aacttaagcg tttctccttt gaaaggtaaa attggta
//
LOCUS       DATRQA010000006        29893 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.006, whole genome shotgun sequence.
ACCESSION   DATRQA010000006 DATRQA010000000
VERSION     DATRQA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29893
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.006"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(29..3127)
                     /locus_tag="VBD27_000212"
     CDS             complement(29..3127)
                     /locus_tag="VBD27_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7024254.1"
                     /translation="MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYS
                     LVTEETKCSCDFFARKGYCQHLAALEHFLKNDPEGKAILSKVQVQQESQQETKKKTSF
                     GSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRDIKAFLN
                     TVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHL
                     SLTRGFFEEGVTLMNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELE
                     IKEKDLKPLFANSYLFYRDTFYHLNLKQEKMVTAIRSLPIEGNLAKHIHFDLDDQDKL
                     AAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLTVHNRQEL
                     EQLTFASHFKHEEKVFKLLEKYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVS
                     LSAKLESYRLIERPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSG
                     QLVIFDEETKKVSATLQGLRARRAKNGHIELDNIAAFQLSELFANQDNVSFSQHFYQL
                     IEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGLGKTLQTIS
                     FLSTKLTRDSRVLILSPSSLIYNWQDEFHKFAPDVDVAVAYGSKIRRDEIIAERHQVI
                     ITSYSSFRQDFETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIE
                     NKLLEIWSIFQIILPGLLPGKKEFLKLNPKQVARYIKPFVMRRRKEEVLPELPDLIEM
                     NYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQICDTPRLFM
                     DYDGESGKLESLRQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKITGSTP
                     ANERHEMTRAFNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAH
                     RLGQKENVEVYRLITRGTIEEKILEMQETKKHLVTTVLDGNETHASMSVDDIREILGV
                     SK"
     gene            complement(3282..4592)
                     /gene="der"
                     /locus_tag="VBD27_000213"
     CDS             complement(3282..4592)
                     /gene="der"
                     /locus_tag="VBD27_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO7024255.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYT
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPLSSVHGIGTGDILDAIV
                     ENLPVEEENENPDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTNFV
                     DSQGQEYTMIDTAGMRKSGKVYENTEKYSVMRSMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHETGKGIIIVVNKWDTIEKDNHTVSQWEADIRDNFQFLSYAPIIFVSAETKQ
                     RLHKLPDMIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYLRFLENQIREAFVFEGTPINLIARKRK"
     gene            complement(4640..5542)
                     /gene="dnaI"
                     /locus_tag="VBD27_000214"
     CDS             complement(4640..5542)
                     /gene="dnaI"
                     /locus_tag="VBD27_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEO7024256.1"
                     /translation="MKSVGQALENQGRVPRNTNDELIQMILADAQVAEFIKTHQLSQR
                     EINISMSKFNQFLIERQKFKNKDSQYIAKGYEPILVMNEGYADVSYLETRELIEAQKK
                     QAISDRINLVNLPKSYRNIRMTDFDINNESRMKAMSQLLDFVETYPSYNHKGLYLYGD
                     MGVGKSYLMAAMARELSERKGVSTTLLHFPSFAIDVKNAISSGTVKDEIDAVKSVPIL
                     ILDDIGAEQATSWVRDEILQVILQHRMLEELPTFFTSNYSFNDLERKWANIKGSDETW
                     QAKRVMERVRYLAIEFHLEGPNRR"
     gene            complement(5539..6714)
                     /locus_tag="VBD27_000215"
     CDS             complement(5539..6714)
                     /locus_tag="VBD27_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO7024257.1"
                     /translation="MSPIDEFTYIKQNKIVYDSNSLIQLYFPIMGSDAMALYDYFVHF
                     FDDGIRRHKFSEVLNHLQYGMPRFQDALVMLTALDLLTVYQATGTYLVKLNQAMSNEL
                     FLSNPIYRRLLEKRIGEVAVAELDMKIPKNARDISKKFTDVFSDLGQPKQEVNRSKNV
                     FDLESFKRLMMRDGLRFNNEKDDVLGIYSVSELYHLNWYDTYQLAKQTAINGMIAPQR
                     MKLQQNEGQHIKDNQSFTNNEKVILRESKNDSALVFLEKIKRSRKAVTTSGEKTLLED
                     LAKMNFLDEVINVMVLYTLNKTKSANLNKAYIMKVANDFAFQNVITAEDAVLKIRDFS
                     DQKVRTKTETKKKQSNVPEWSNPDYKDEVSPEKEIELEQFKTDALKRLERLGKDGES"
     gene            complement(6714..7193)
                     /gene="nrdR"
                     /locus_tag="VBD27_000216"
     CDS             complement(6714..7193)
                     /gene="nrdR"
                     /locus_tag="VBD27_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEO7024258.1"
                     /translation="MRCPKCGYNKSSVVDSRQAEEGTTIRRRRECEKCGNRFTTFERL
                     EELPLLVIKKDGTREQFSRDKILNGIIQSAQKRPVSSEDIENCILRIERKIRSEYEDE
                     VSSITIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITKRVRSKKSGSV"
     gene            complement(7342..8847)
                     /locus_tag="VBD27_000217"
     CDS             complement(7342..8847)
                     /locus_tag="VBD27_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEO7024259.1"
                     /translation="MKNKKDQFIGVKQPLSKKLSQLVFILFFSLFTVFSVLVYTSATR
                     YVLHREKINVGRSLEKTRVRLSQANSSLTSDDILEILYNQVFADDIYPHKRQNGIVRT
                     GESIDSILYVNQEMTLYDVNRKPVFSTLRTGMPTIGKSMGKVIISKVADMEGFVGTKA
                     IYSQKTGQLLGYVQIFYNLGRYYSMRQNIIVFLIMMEVLGTVLALVVINSATKRIVRP
                     VKNLHDLMHQISENPSNLEIRSKVRSEDEIGELSRIFDGMLDQLEDYTRRQSQFISDV
                     SHELRTPVAVVKGHIGLLQRWGKDDPEILEESLAAAYHEADRMSLMINDMLNMIRVQG
                     SLELHQDEVTDLSSSISVVIENFRILREDFQFIFENNISDIVWGKIYKIHFEQALMIL
                     IDNAIKYSPSYKEVSVVLSVDNDFATVVVKDKGEGISDEDIEFIFDRFYRTDKSRNRE
                     STQAGLGIGLSVFKQIMDAYHLKVDIKSELNQGTEFIVRIPIKKFEETKEI"
     gene            complement(8837..9526)
                     /locus_tag="VBD27_000218"
     CDS             complement(8837..9526)
                     /locus_tag="VBD27_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7024260.1"
                     /translation="MGKKILIIEDEKNLARFVSLELLHEGYDVVVETNGREGLDTALE
                     KDFDLILLDLMLPEMDGFEITRRLQAEKTTYIMMMTARDSVMDIVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQEIETKTKEKGDSGSFRDLSLNTHNRSAMRGDEEISLTKRE
                     FDLLNVLMTNMNRVMTREELLEHVWKYDVAAETNVVDVYIRYLRGKIDIPGRESYIQT
                     VRGMGYVIREK"
     gene            complement(9761..10294)
                     /locus_tag="VBD27_000219"
     CDS             complement(9761..10294)
                     /locus_tag="VBD27_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEO7024261.1"
                     /translation="MLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHV
                     VYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEKQTINEVFIEAENVSTKKELVDQELV
                     LILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYEEKQAKE
                     KEKSNPFAALEGMFDSD"
     gene            complement(10399..11289)
                     /gene="htpX"
                     /locus_tag="VBD27_000220"
     CDS             complement(10399..11289)
                     /gene="htpX"
                     /locus_tag="VBD27_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO7024262.1"
                     /translation="MLYQQIASNKRKTVVLLIVFFCLLAVIGAAVGYLVLGSYQFGLV
                     LALIIGVIYAVSMIFQSTNVVMSMNNAREVTEDEAPNYFHIVEDMAMIAQIPMPRVFI
                     VEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAV
                     ALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLA
                     ISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGL
                     KSLFYTHPPIADRIERLRHM"
     gene            complement(11310..11864)
                     /locus_tag="VBD27_000221"
     CDS             complement(11310..11864)
                     /locus_tag="VBD27_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEO7024263.1"
                     /translation="MGTMILIAIIALFVIWLIVAYNSLVRSRMHTKESWSQIDVQLKR
                     RNDLIPNLIETVKGYAAYEGKTLEKIAELRAQVAKANTPAEAMTASNELTRQLSSILA
                     VAENYPDLKANNSFVKLQEELTNTENKISYSRQLYNTTTSNYNVKLETFPSNIVGKLF
                     GFKPSQFLETPEEEKEVPKVSFDF"
     gene            11992..12705
                     /gene="rsmG"
                     /locus_tag="VBD27_000222"
     CDS             11992..12705
                     /gene="rsmG"
                     /locus_tag="VBD27_000222"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEO7024264.1"
                     /translation="MTPQAFYQVLIEHGITLTDKQKKQFETYFRLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGYIDNSPLSILDIGAGAGFPSIPMKILYPEIDITIIDS
                     LNKRINFLNILANELELSGVHFFHGRAEDFGQDKVFRAKFDIVTARAVARMQVLAELT
                     IPFLKVNGRLIALKAAAAEEELISAEKALKTLFSQVTVNKNYKLPNGDDRNITIVSKK
                     KETPNKYPRKAGTPNKKPL"
     gene            12720..14099
                     /locus_tag="VBD27_000223"
     CDS             12720..14099
                     /locus_tag="VBD27_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO7024265.1"
                     /translation="MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKT
                     HYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQIVAILLMQTGGLGLVTLMSLSYYTLR
                     RKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPRFGLGHG
                     IFNSIFLAVSAFCNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEA
                     TIQTSHKEPKLIKTFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLP
                     KQLMVSFFQTVTMRTAGFSTIDYTQTDFATNLVYIIQMLIGGAPGGTAGGFKVTVIAI
                     LLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLELNPHIDPF
                     SLFFEASSALATVGVTMNTTNQLTLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIH
                     YAETEIILG"
     gene            14112..14783
                     /locus_tag="VBD27_000224"
     CDS             14112..14783
                     /locus_tag="VBD27_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEO7024266.1"
                     /translation="MKTKIIGVLGLGIFGQTLAQELSNFEQDVIAIDSNPENVQAVAE
                     VVTKAAIGDITDLAFLKHIGISDCDTVIIATGNSLESSVLAVMHCKKLGVPQVIAKAR
                     NLVYEEVLYEIGADLVISPERESGQNVAANLMRNKITDVFQIESDISVIEFKIPKSWV
                     GKTVEQLNIRHKFDLNLIGIRKAKNKPVDTEVPINTPLEEGIILVAIANSDAFQRYDY
                     LGYFN"
     gene            complement(14818..15648)
                     /locus_tag="VBD27_000225"
     CDS             complement(14818..15648)
                     /locus_tag="VBD27_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO7024267.1"
                     /translation="MAKQLIGYQEGYGFLYRLSGASKLLFFILVSVACMTTYDTRFLI
                     VVSLGSLVLFRLAKIKWQQVSFVMTLVVVFAVLNIIMVYLFAPHYGDKIYGSSSLLLK
                     GIGPYDVTSQELFYLFNLILKYFCTVPLALLFLMTTNPSQFASSLNQLGLSYKIAYAV
                     SLTLRYIPDVQEEFYTIRRAQEARGIELSKKSNLVARIKGNLQIVTPLIFSSLERIDT
                     VATAMELRRFGKNKRRTWYSKQSLEKSDIVLIILALASLFVSLYLIHLNHGRFYNPWQ
                     "
     gene            complement(15641..17317)
                     /locus_tag="VBD27_000226"
     CDS             complement(15641..17317)
                     /locus_tag="VBD27_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7024268.1"
                     /translation="MKDFIEWKDFTFQYDVQSEPTLKGINLSIPKGEKVLILGPSGSG
                     KSTLGHCLNGIIPNTHKGQYSGIFTINHKNAFDLSIYDKSHLVSTVLQDPDGQFIGLT
                     VAEDIAFALENDVVAQEEMASIVEMWAKRLEIAPLLSKRPQDLSGGQKQRVSLAGVLV
                     DDSPILLFDEPLANLDPQSGQDIMALVDRIHQEQDATTIIIEHRLEDVLYERVDRVVL
                     FSDGQIIYNGEPDQLLKTNFLSEYGIREPLYISALKNLGYDFEKQNTMTSIDDFDFSE
                     LLIPKMRALDLDKHTDKLLSVQHLSVSYDLENNTLDDVSFDLYKGQRLAIVGKNGAGK
                     STLAKALCQFIPNNATLIYNNEDVSQDSIKERAERIGYVLQNPNQMISQAMVFDEVAL
                     GLRLRGFSDNDIESRVYDILKVCGLYQFRNWPISALSFGQKKRVTIASILILNPEVII
                     LDEPTAGQDMKHYTEMMSFLDKLSCDGHTIVMITHDMQLMLEYTDRAIVIDNGLIVAD
                     DHPINILSNTELLKKTHLKKTSLFALADRLGISPQKLTQWYIDNQGGKNG"
     gene            complement(17334..17882)
                     /locus_tag="VBD27_000227"
     CDS             complement(17334..17882)
                     /locus_tag="VBD27_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEO7024269.1"
                     /translation="MNTNTIKKVVATGIGAALFIIIGMLVNIPTPIPNTNIQLQYAVL
                     ALFAVIYGPGVGFFTGFIGHALKDSIQYGSPWWTWVLVSGLLGLMIGFFAKKLAIQLS
                     GMIKKDLLLFNVVQVIANLIGWSVVAPYGDIFFYSEPASKVFAQGFLSSLVNSITIGV
                     GGTLLLLAYAKSRPQKGSLSKD"
     gene            complement(18051..19259)
                     /gene="sstT"
                     /locus_tag="VBD27_000228"
     CDS             complement(18051..19259)
                     /gene="sstT"
                     /locus_tag="VBD27_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEO7024270.1"
                     /translation="MKRFLKAWRKTSLIKKITIGVVIGLFLGILVPKASAIGLLGQLF
                     VGGLKAIAPLLVFTLVISALSQHREGGKTNMSTIIGLYITATFAAALIAVVVNYIFPL
                     TLILKTPAKTDLLPPKGISEVFQSLLLKIVDNPIHAITEANYMSILFWAVIFGLAMRS
                     SNQRTKDLMQTFADATSQVVKWIINLAPIGIMGLVFTSISENGIGILGDYGLLILVLV
                     GTMLFVALVVNPIIAFVMMRKNPYPLVLRCLKDSGITAFFTRSSAANIPVNMRLCEDL
                     GLDKDTYSVSIPLGAAINMAGAAITINILTLAAVNTLGITVDFPTAFLLSVVAAVSAC
                     GASGVTGGSLLLIPVACSLFGISNDVAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     VAEKSVFGKK"
     gene            19435..20802
                     /gene="brnQ"
                     /locus_tag="VBD27_000229"
     CDS             19435..20802
                     /gene="brnQ"
                     /locus_tag="VBD27_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO7024271.1"
                     /translation="MKLKNTYIIIGLMLFALFFGAGNLIYPAYLGIYSGSNWAWAIIG
                     FCLTGVTLPLLGVAAVTYSGATDVESFTKPVSKTYAIFFAVALYLSIGPFFAIPRTGA
                     TSFSVGIQPILGDSSLVKIIYGLIFFGISYFLAIRPSQIVDRIGKYLTPFLLTVISIL
                     IIASLIHPAAIVGTPHNASTNVSDAFKDLPFLAGLIQGYGTMDALASIAFAIIVIQAS
                     KQYGAITKKEITSMTLKSGAIATFLLAFIYIFVGRIGATSQSLFKFANGSFLLHNTPI
                     DGGHVLSQSANFYLGIVGQAILGTAIFLACLTTATGLITACAEYFHKLLPKISHITWA
                     TIFTLIAITFYFGGLSEIIRWSLPVLYLLYPLTIVLIFLVFFDQKFESSRIVYQTSIA
                     ATAVAALYDALSKLGEMTGLFTIPSALTTFFTKVVPLGEYSMGWISFAICGVLVGLIL
                     KKVKA"
     gene            20916..21971
                     /locus_tag="VBD27_000230"
     CDS             20916..21971
                     /locus_tag="VBD27_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO7024272.1"
                     /translation="MRAATYLSANELSLTDKAKPQVIKPTDAVVRLVKTTICGTDLHI
                     LGGDVPACKPGTTLGHEGIGIVEEVGSAVNHFKVGDKVIISCVTACNTCYYCKHQLPS
                     HCEDGGWILGHLIEGTQAEYVHIPHADGSLYHAPEGVCDDALVMLSDILPTSYEIGVL
                     PSHIKPGDTVCIVGAGPIGLSALLTAQFYSPAKIIMVDLSQKRLEASKKFGATHTILS
                     TSTQEVKEEIDKITKGRGVDVVLECVGYPATFDICQNVVSIGGHIANVGVHGKPVEFN
                     LQDLWIKNITLNTGLVNANTTEMLLEVLETGKIDATQLVTHHFKLSEIEEAYKVFKAA
                     EENNTLKVIIENDITPA"
     gene            complement(22020..22712)
                     /locus_tag="VBD27_000231"
     CDS             complement(22020..22712)
                     /locus_tag="VBD27_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7024273.1"
                     /translation="MIEWIQTHLPNVYQMGWEGAYGWQTAIVQTLYMTFWSFLIGGLM
                     GLLGGLFLVLTSPRGVIANKLVFGVLDKVVSVFRALPFIILLALIAPVTRVIVGTTLG
                     SPAALVPLSLAVFPFFARQVQVVLAELDGGVIEAAQASGGTLWDIIVVYLREGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGSVAITKGYNYSRDDITLVATILILLLIFFIQF
                     LGDFLTRRLSHK"
     gene            complement(22709..23779)
                     /locus_tag="VBD27_000232"
     CDS             complement(22709..23779)
                     /locus_tag="VBD27_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEO7024274.1"
                     /translation="MSKEPIIKLDNIDVTFHQKKREINAVKDVTIHINQGDIYGIVGY
                     SGAGKSTLVRVINLLQEPSAGKITIDDQVIYDNKVTLTSTQLREQRREIGMIFQHFNL
                     MSQLTAEQNVAFALKHSGLSKEAKAAKVAKLLELVGLSDRAQNYPSQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQDLNKKLGLTIVLITHEMQIVKDIAN
                     RVAVMQNGKLIEEGSVLDIFSHPRESLTQDFIKIATGIDEAMLKIEQQEVVKNLPVGS
                     KLVQLKYAGHSTDEPLLNQIYKEFEVTANILYGNIEILDGIPVGEMVVILSGDEEKLR
                     QACQAITDSQVQLTLLKEGGKA"
     gene            complement(23772..25148)
                     /locus_tag="VBD27_000233"
     CDS             complement(23772..25148)
                     /locus_tag="VBD27_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO7024275.1"
                     /translation="MTLTNEENQIQKFLSDTIVQDDINILRDLIAIKSIFAQKVGLND
                     LSSYLGEVFRKAGAEVIIDDSYSAPFIVANFKSSKVDAKRIIFYNHYDTVPADEVEQW
                     TEDPFTLSLRYGKMYGRGVDDDKGHITARLSAVKKYLSRHKGELPLDITFIVEGAEES
                     ASVGLDYYLEKYQEQLQGADLIVWEDGPKNPKGQLEIAGGNKGIVTFDLSVSSADVDI
                     HSSFGGVVDSSTWYLIQALNTLRDNKGHILVEGIYDKVIPPTKRELELVEKYSYRSAK
                     ALEGAYQLVLPSLADSHKTFLRKLYFEPSIAIEGITSGYQGEGVKTILPAYAKCKAEV
                     RLVPGLTPKGVLDSIQNHLKENGFKDIELTYTLGEMSYRSDMSAPSILKVVDLAEQFY
                     PEGISLLPTSPGTGPMYLVHQALRAPIAAIGIGHANSRDHGVDENVSIADYYTHIELV
                     EALIESYE"
     gene            complement(25282..26106)
                     /locus_tag="VBD27_000234"
     CDS             complement(25282..26106)
                     /locus_tag="VBD27_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO7024276.1"
                     /translation="MKLKHIVLGLALTTLLGVTFSNQEVSASSTSSKVVKVGVMTFSD
                     TEKARWDKIEKLVGDKAKIKFTEFTDYTQPNQATANKDVDINAFQHYNFLENWNKENK
                     KNLIPLEKTYLAPIRIYSEKVKSLKKLKKGATIAIPNDATNGSRALYVLQSAGLIKLN
                     VSGKKVATVANITSNKKDINIQELDASQTPRALKDVDAAIINNTYIEQANLKPSDAIF
                     VEKSDKNSKQWINIIAGRKNWKKQKNAKAIQAILDAYHTDEVKKVIKDTSADIPQW"
     gene            complement(26247..27080)
                     /locus_tag="VBD27_000235"
     CDS             complement(26247..27080)
                     /locus_tag="VBD27_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7024277.1"
                     /translation="MTIKKVLSVTGIILVTVASLAACSSKSHTTKTGKKEVNFATVGT
                     TAPFSYVKDGKLTGFDIEVAKAVFKGSDNYKVTFKKTEWSSVFTGIDSGKFQMGGNNI
                     SYSSERSQKYLFSYPIGSTPSVLAVPKNSNIKAYNDISGHKTQVVQGTTTAKQLENFN
                     KEHQKNPVTLKYTNENITQILTNLSDGKADFKLFDGPTVNAIIKNQGLTNLKTIPLTM
                     RDQPYIYFIFGQDQKDLQKYVNNRLKQLRKDGTLSKIAKEYLGGDYVPNEKDLVTPKE
                     K"
     gene            complement(27182..27871)
                     /locus_tag="VBD27_000236"
     CDS             complement(27182..27871)
                     /locus_tag="VBD27_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEO7024278.1"
                     /translation="MLTKPIIGITGNEREMSDIPGYYYDSVSRHISEGVKNAGGLPVI
                     LPISEAESAKAYVEMIDKLIISGGQNVLPSYYGEEKIIESDDYSLARDIFEFALVEEA
                     LKQNKPIFAICRGMQLVNVALGGTLNQSIDNHYQEPYIGFAHYLNVEKGSFLEGFISG
                     DFKINSLHRQSVKLLAEGLIVSARDPRDGTVEAYESRTEQCIIGVQWHPELMLHQIEN
                     QTLFGYFVNET"
     gene            complement(28026..28742)
                     /locus_tag="VBD27_000237"
     CDS             complement(28026..28742)
                     /locus_tag="VBD27_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7024279.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKQGEPDPESN
                     SALKFVLDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADTVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            complement(29168..29872)
                     /locus_tag="VBD27_000238"
     CDS             complement(29168..29872)
                     /locus_tag="VBD27_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024280.1"
                     /translation="MRLVWKFLSEKLVCKIMDSSYGVLILFFICLSITAVGGYTLVYN
                     LSGYSSMDAQKVFGIIVLTIILLVFIVGTIFYAIGSAQLIMSILSKKKISFDEKDAPL
                     DGATHVGETVFQGKEVSLYSKQDKFFLWSRKWQKGFSKYQYYHIRPVDQTEISEVYQV
                     ERCCFYCDHIFTQFFIREGKNREKIVELCTIDVQIGNDRDLGFKQVNRGEFEKTVPFK
                     DVEIKEIKKLIDLKQI"
BASE COUNT         9508 a         5943 c         4540 g         9902 t
ORIGIN      
        1 tttttatatt atagtcacat catcatattt atttagaaac tccaagtatc tctcgaatat
       61 catctacgct catgctagca tgggtttcat ttccatccag aactgttgta accaaatgtt
      121 ttttagtttc ttgcatttct agaatttttt cttcgattgt gcctctagta atcaagcgat
      181 aaacctcgac attctctttt tgccccaacc tatgtgcgcg actaatcgcc tgcatttcaa
      241 cagctggatt ccaccacaag tcaatcagaa caacggtatc agctcctgta aggttgagac
      301 caacgccacc agcttttaaa gaaatcaaga aagcatcttt tgatcctgca ttaaatgctc
      361 tggtcatttc atgacgttca tttgctggtg tagaaccagt aattttataa gttgtaagcc
      421 ccatagctac catctcacgt tctgcgatat ctaacatacc cctgaactga gaaaaaatca
      481 gagcacgatg tccattttcc ttaatttgtg ttaaaagttg acgtaggctt tctaacttac
      541 cgctttcacc atcataatcc ataaaaagac gtggagtgtc acaaatttga cgtaatcgtg
      601 tgatgccaga taaaatttca attttacgac gatttaagtc agcgtcacta gaatgttgga
      661 tactttcttg aatttggcgt aattgcgcca aatagataac tttttgactg tctgtcattt
      721 catttggata gttcatttca atcaaatccg gtaattctgg gagaacttct tcttttcgac
      781 gtctcatcac aaatggtttg atataacgag caacttgttt agggttcaac ttcaaaaact
      841 ctttcttacc aggtaataaa ccaggtaaaa tgatctggaa aatggaccaa atttctaaca
      901 gtttattctc aataggtgtt cccgataatg caaaacagtt tttgacctca aaagatctca
      961 agctatgggc tattttagtt tgagcatttt tcataacctg tgcttcatcc aaaattaaat
     1021 aatcataatt tccttcagaa taagtctcaa aatcctgcct aaatgatgaa taacttgtaa
     1081 tgattacttg atgacgttca gcaataattt catctctacg aatcttagaa ccataggcaa
     1141 ctgctacatc tacgtctggc gcaaatttgt ggaattcatc ttgccaatta taaattaaac
     1201 ttgatggtga taaaataagc acacgagaat ctctagttag ttttgtactt aaaaatgaaa
     1261 tcgtttgaag tgtttttcca agccccatat catcagctaa aatccctgca aagccataat
     1321 gatctaacat acttaaccaa cggacacctg taagctgata atcgcgcaag gaagctgaaa
     1381 cagatagacc aggtatttta aacttttctg ggtgtctcaa atcttcaatc aattggtaaa
     1441 aatgttgtga aaagctaaca ttatcttgat ttgcaaacag ttctgataat tgaaatgctg
     1501 caatattatc taattcaata tgcccattct ttgctcgtct agctctcaac ccttgcaaag
     1561 ttgcacttac tttcttagtt tcttcatcaa aaataactaa ttgccctgat ttattcacaa
     1621 aataaggatt attgtcaaac aaagctacca gagcttgatc aacatcgttt tccagtaaat
     1681 cagagaaatc aaaagaaata tctagcaggc ttcccttagc ttcaatatca atttgcggtc
     1741 gttctatgag acgataactt tccaattttg cggataagct cactgttccc atttttttaa
     1801 attgtggaag catataggta taaaaatcgt ataattcttg tgcactgtat gctggatggc
     1861 tggttgaaaa gtggggtgca aagccatatt tttctaagag tttaaatact ttttcttcat
     1921 gtttgaagtg actagcaaat gttaactgtt ctagttcttg acgattatga acagttaaat
     1981 cattaccata atcaaatacc atttgcaata aaatttcatt ttgactatta atatcgaact
     2041 caaaatttac cttaaaatca tgaatagaga agctccgtgg agcatccact agtccaatct
     2101 ccttaaaatc cagtaaatga gctgctaact tatcttgatc atctaaatca aaatggatat
     2161 gttttgctaa atttccctca atgggaagac tacgaatagc cgttaccatc ttttcttgct
     2221 tcaaattaag atggtagaaa gtatcgcgat aaaacaaata agaattcgca aatagtggtt
     2281 ttaagtcttt ctctttgatt tctaattcaa ttgattgcct atggacaata accttaaatt
     2341 gataaagatg atcctcacct tctaattcct taaagtagag gtgattaaat tgatgaaaat
     2401 cactttcaaa tgaaaaattt tctaaagcat tcatcaaagt aacaccctct tcaaaaaaac
     2461 cacgagtgag actaaggtga cgaccttgat ttggtaaaat aaactctaga tcaattttgc
     2521 ttgaatgact tggaatgagc ctccataaaa attctatcaa ttcttgactg gtctcatcaa
     2581 actgtatcaa agacaaagtt tcaaagtatt gttttccaat ctgataataa gcttcttttc
     2641 gaacagtatt taaaaaggct ttgatgtcac gaataacata acttctatca tcaggcagtc
     2701 gtctaatttt taaagtccac cagatatcat tagcataagg attttgttct ccttgagccg
     2761 ataattgata ttttatagta tcatcttcat taataattag actatctaaa aaaacactac
     2821 caaaagaggt tttcttctta gtttcttgtt gactttcttg ttgaacttga actttagata
     2881 agattgcttt accttctgga tcgtttttca agaaatgctc cagagcagct aaatgttgac
     2941 aatagccttt tcttgcaaaa aaatcacaag aacattttgt ttcttctgtt actaatgaat
     3001 attcaatctg acaatcccca actttagctt ttaatagatt accttcttga gaaatcaagg
     3061 acaccaaacc ttgttcgtag agttctatac cttgatttcg aatacgtcct ggaatcattc
     3121 tactcattat gctaccacct tcatcttatc ccttaattat aacaaaattc aagtccaatt
     3181 tcttgtaaaa atactaaatt gattgttttt tgatacagta acaaataaaa aaggctaaaa
     3241 caactgtcct aaccttctat ttcaatttca acaataagat gttatttacg tttacgtgct
     3301 attagattaa ttggtgttcc ttcaaaaaca aatgcttcac ggatttgatt ttctaagaaa
     3361 cgaagatatg aaaagtgcat caattcttct tcgttaacaa aaacaacgaa agttggtggt
     3421 ttaacagcaa cttgagttgc gtagaaaatc ttaagtcgtt ttcctttatc agttggtgtt
     3481 ggattaatag caatagcatc cataatcaca tcatttaata cagcagacgg aatacgtttg
     3541 ttttgacttt cgctaatacg tttaatcata tctggtaact tgtgcaaacg ttgctttgtc
     3601 tctgcagaaa caaagataat tggcgcataa gacaagaatt ggaagttatc acgtatatca
     3661 gcttcccact gactgacagt atgattgtct ttttcgatag tatcccattt attaactaca
     3721 ataataatac ctttaccagt ttcatgtgca aaccctgcaa tacgcttgtc atactcacga
     3781 ataccttcct ctgcattaat caccataaga acaacatctg agcggtcgat ggcgcgcata
     3841 gaacgcataa cagaatattt ctctgtgttt tcatatacct taccagattt acgcatacca
     3901 gcagtatcaa tcatagtata ctcttgcccc tggctatcaa cgaaattagt atcaatagca
     3961 tcgcgcgtag taccagcaac tgggctagca ataacacggt cttccccaag gatggcatta
     4021 attaaactag atttcccaac atttggacgt ccaatcaaac taaaacgaat aatatctgga
     4081 ttttcatttt cctcttctac cggaagattt tccacaatag catccaatat atcaccagta
     4141 ccaataccat gaactgatga caagggataa ggatccccta aaccaagaga gtagaaatcg
     4201 taaatatcat tgcgcatctc aggattatct accttattaa cagctaaaat aaccggttta
     4261 ttggtcttat aaagaatacg tgagacatac tcatctgcat cagtcacacc ttctttaccc
     4321 gacacaacaa acacaataac atcagcctct gtcatagcaa tgtctgcttg gtgcttaatt
     4381 tgctccataa acggtgcatc aacatcatca atcccaccag tatcaattaa tgaaaactta
     4441 cgatttagcc actcacccgt tgtatagata cgatcacgag tgactccttc aacgtcctca
     4501 acaatggaaa tacgttctcc tgcaattctg ttaaataatg ttgattttcc aacattcgga
     4561 cgaccaacaa tagcaactgt aggtaatacc ataattctcc tctcactatt gggaatactc
     4621 ccaattccat tctattttat taacggcgat tcggtccttc taagtggaat tctatcgcta
     4681 aataccgtac gcgctccata actcgttttg cttgccaagt ttcgtcgctt ccttttatat
     4741 tagcccattt tctttccaaa tcgttaaaac tataatttga cgtaaagaaa gtgggcaact
     4801 cttctagcat acgatgttgt aatataactt gtaaaatctc gtcacgaacc caggacgttg
     4861 cctgttcagc cccaatatca tctaaaatca aaatcggaac acttttcaca gcatcaattt
     4921 catcttttac agttccgctt gaaatagcat ttttgacatc aatagcaaaa ctagggaaat
     4981 gaagaagagt cgttgacaca ccctttctct ctgataattc cctcgccatt gctgccatta
     5041 aataggattt cccaaccccc atatctccat ataaataaag ccccttgtga ttatatgaag
     5101 gataagtctc tacaaaatca agcagttgtg acatagcttt cattcgactc tcgttattaa
     5161 tatcaaagtc agtcattctg atattccgat aggacttggg tagattaact aaattaatac
     5221 gatcactgat agcttgtttt ttttgtgctt caattaactc tcgtgtttct aaataagaaa
     5281 catctgcata accttcattc ataaccaaaa taggctcata gcctttagca atatattgac
     5341 tatccttatt tttaaatttc tgacgctcaa tcaaaaattg attaaattta gacatgctga
     5401 tatttatttc acgctgagaa agttggtggg ttttaatgaa ctccgctacc tgagcatctg
     5461 ctaaaatcat ttgaataagc tcatcatttg tattacgagg cactctccct tgattttcta
     5521 atgcttgtcc cactgatttc atgattcacc atcctttcct aatcgttcta aacgcttgag
     5581 ggcatctgtt ttaaactgtt ctaattcaat ttctttttct gggctaacct catctttata
     5641 atcaggatta ctccattcag gaacattcga ttgtttcttc ttcgtttctg ttttagtcct
     5701 tactttttga tctgaaaaat cacgaatttt aagcacagca tcttcggccg ttataacatt
     5761 ctgaaaggca aaatcattag caactttcat gatataggcc ttatttaagt tagcagattt
     5821 tgtcttattc aaggtatata aaaccataac attaatgact tcgtctagga aattcatttt
     5881 tgccaaatcc tcaagtaaag tcttctcacc acttgttgtc actgccttac ggcttctctt
     5941 gattttttca aggaacacta gagcactatc atttttagat tcccttagta tgaccttttc
     6001 attatttgta aaactctgat tatctttaat atgctgcccc tcattttgtt gaagtttcat
     6061 tcgctgaggc gcaatcattc cattgatagc tgtttgtttt gctaattgat aggtatcata
     6121 ccaatttaaa tgataaagtt ctgatacact ataaatacct aagacatcat ctttttcatt
     6181 gttaaaacgt aagccatctc gcatcatcaa acgtttgaaa ctttccaagt caaaaacatt
     6241 tttactccgg tttacctctt gttttggttg tcctaaatcc gagaaaacat ctgtaaattt
     6301 tttagagata tctctagcat ttttaggtat tttcatatct aattctgcca ctgctacctc
     6361 tcctatacgt ttttctaaca aacgacgata gatgggatta gataaaaata actcatttga
     6421 catagcttga ttcaacttaa caaggtaagt acctgtggct tggtaaacag tcaacaaatc
     6481 caaagctgtt aacattacca atgcatcttg aaatcttggc atcccgtatt gcagatgatt
     6541 gagaacctct gaaaatttat gacgtcgtat tccgtcatca aaaaaatgaa caaaataatc
     6601 atagagtgcc atggcatcac ttcccataat tgggaaatac aattgtatta aactattact
     6661 atcataaaca atcttatttt gcttaatata tgtaaactca tctattggac tcatcacacg
     6721 ctaccactct ttttactccg tacgcgcttt gtaatttgtt gaagtagttc ttcaatttca
     6781 tctacatctt taaaagattt ataaacacta gcaaagcgca cataagtaat ctcatctaat
     6841 tcggcgagtt catccatcac taaatttccg atagtgatac tagaaacctc atcttcatac
     6901 tcactcctaa ttttacgttc gatacgtaag atacaatttt caatatcttc gctcgaaaca
     6961 gggcgttttt gagcactctg aataatacca ttcaaaattt tatctcgaga aaattgctcg
     7021 cgagtaccat ctttctttat gactaaaaga ggcaattctt ccaaacgttc aaatgttgta
     7081 aagcggttgc cacatttttc gcactctctc cggcgtctga tagtcgtacc ttcttcagct
     7141 tgtcggctat caaccacact agatttatta tagccacatt ttggacaacg catgtcaaca
     7201 cccctattct atcatatcat ttaatcatct tattttaaca caaattcagt aataacaaaa
     7261 gaagcgcctc tcttatcacc tcatcatatc aaaacacaaa ataatcctta tgccttttat
     7321 cattaagcat aaggattttt cttatatttc tttagtttct tcaaattttt ttataggaat
     7381 gcgaacaatg aattcagtcc cttgatttag ttcactttta atatccacct ttaaatggta
     7441 agcatccata atttgtttaa acactgaaag cccgatacct aaaccagctt gtgtactttc
     7501 tctgttacgt gatttatcag ttcgataaaa acgatcaaag ataaactcaa tatcttcatc
     7561 tgagatacct tctcctttat cttttacaac tactgtcgca aaatcattgt ctactgataa
     7621 aacaacactg acttctttat aactaggaga gtacttgata gcgttatcaa ttaagatcat
     7681 gagagcttgc tcaaaatgta tcttatatat ctttccccaa acgatatctg aaatattatt
     7741 ttcaaaaata aattgaaaat cttctctcaa aatacggaaa ttttcaataa caactgatat
     7801 agagcttgat aaatctgtca cttcatcttg atgaagttcc aagcttcctt ggacgcgaat
     7861 catatttaac atgtcattaa tcattaagga catacgatct gcctcatggt atgccgctgc
     7921 taaactttct tcaagaattt ctggatcatc tttcccccaa cgttgtaata aaccaatatg
     7981 accctttaca acagcgacag gcgttcttaa ttcatgacta acatcactaa tgaattgtga
     8041 ttgtcgacga gtatagtctt ccaactgatc caacatccca tcaaaaatcc tagacaattc
     8101 tccaatttcg tcttctgaac ggaccttact tctaatttct aaattactag gattttcaga
     8161 tatctgatgc attaagtcgt gtaaattttt aaccggacga acaatacgct tagtagcact
     8221 attaataacg accaaggcta aaacagtccc taaaacttcc atcataatca agaaaacaat
     8281 gatgttttgc ctcatactat aataacgacc taaattatag aaaatttgaa cgtatccaag
     8341 caactgtcct gttttttgag agtagattgc ttttgtacca acaaaacctt ccatatctgc
     8401 aactttagag atgataactt ttcccatgga tttaccaata gttggcatac ccgttctcag
     8461 tgtagaaaaa actggtttcc tgttgacatc gtatagggtc atttcttgat taacataaag
     8521 aatactatcg attgactcgc ccgttctaac aattccattt tgacgcttat gaggatagat
     8581 gtcatccgca aaaacctggt tgtacaggat ctctaaaata tcatcggatg tcaaactaga
     8641 gttcgcttgc gacaaacgaa ctctagtttt ttctaatgac ctaccgacat taattttttc
     8701 cctgtgaagg acgtatcttg ttgccgaagt ataaactaaa acagaaaata ctgtaaatag
     8761 gctaaaaaat aagataaaaa ctaattgcga taattttttt gacagaggtt gttttacccc
     8821 tataaactga tcttttttat ttttcacgaa tcacatagcc cattccgcga acagtttgaa
     8881 tatatgattc acggcctggg atatcaattt tacctcttag gtaacggata taaacatcaa
     8941 caacgtttgt ctctgctgcc acatcgtatt tccaaacatg ttctagcaac tcttctcgtg
     9001 tcataacacg attcatattt gtcatcaaga cattcaacaa atcaaattca cgttttgtta
     9061 gtgaaatttc ctcatcacca cgcatcgctg aacgattatg agtatttaat gacaaatcac
     9121 ggaaactacc actatcacct ttttcttttg tcttcgtttc aatttcttgg cgtcggaaaa
     9181 tcgctctaac acgtgctaat aattcttcga ttgcaaacgg cttaacaata taatcatctg
     9241 ctccacgatc aagaccagct acaatatcca taacagaatc acgtgctgtc atcatcatga
     9301 tataggttgt tttttcagcc tgcaggcgac gtgtgatttc gaaaccatcc atctctggaa
     9361 gcattaaatc cagtagaatc aaatcaaaat ctttttctaa tgctgtgtcc aatccttcac
     9421 gaccgtttgt ttcaacaaca acatcatatc cttcatgtag tagttctaac gagacgaagc
     9481 gagctaaatt tttctcatct tcgattatta agatcttttt acccatctac accaaccttc
     9541 ttattcttta cttaacacta tcaattattt tttagaatat ctagataaaa aacaagatag
     9601 cccacatttt actaacgttt ctttaaataa ataaatcgcc atcccaaaca actgttcgat
     9661 agcgagactt aatttttatt attgagtagt gtcctctgtc acttttcaat tctactaaaa
     9721 aaaaattaaa atatcaactg atgttattaa taatttttat ctaatcacta tcaaacatac
     9781 cttctaaggc tgcgaaagga ttagattttt ctttttcttt agcttgtttt tcttcatact
     9841 gcttttcagt cattaacgac caatttttac ctgataaatc cgcttctacc ccaacctcat
     9901 cagctgccag aacacgaaga ggtatattta acaagatatt atcgataacg ctttcttcta
     9961 aattaatatc atcttcctct aatattaaga ccaactcctg atcaacgagc tcttttttag
    10021 tgctaacatt ttcagcttca ataaaaactt cattaatagt ttgcttttca gataaaacaa
    10081 ctggtttcat agaacgacta gacggtaatg taatatcgta agccatatta tagtcaagta
    10141 gatagagacc atcttcatac accacatgac ctgaaacttg aatatcagaa atatccatta
    10201 tctctgaatg acgctccata agcgactctt tgatatctat ttttttatca aaataaagcc
    10261 cctctggtga ttttttgatt tctgtaagta acacgtgcca tctcccttta tagtatatgt
    10321 catattatat caagttttac aggctttgtc ttaaaaaaac aaaacgcaag gcatagctac
    10381 cctacgtttt gttaacgact acatatgacg caaacgctca atacgatctg caattggtgg
    10441 atgcgtataa aataaagact ttaacccttc ttttttcgtt gggtcattga tatagagagc
    10501 cgcactagca tcatctactg gatgccccat aggttcagaa cgatcaagtt tttctaaagc
    10561 agagatcatt ccttgtggat ttcttgttaa ctcaacagaa ctagcgtctg ctaagtattc
    10621 gcgctgtctt gaaatagcta attgaactaa actagcggct aatggtgcaa ggattagtga
    10681 taaaatagag aaaatcaaaa ccaagatgtt tccaccatct tctctgtcat catcacgcct
    10741 acgacctccg ccgtaaaata acatacggct accaatgctt gatattagag ttacagcact
    10801 cgctaaagct actgcaatgg ttgaaatacg gatatcataa ttacgaatat gactaacctc
    10861 atgtccaata acaccctcta gctcttcgcg gttcataaca gctaataggc ctgtcgttgc
    10921 tgcaacagca gcattttctg gcttagagcc tgtggcaaag gcatttaggg agtcatcttc
    10981 aacaatgaaa acgcgtggca taggtatctg agctatcatt gccatatctt caacaatatg
    11041 aaaataattt ggcgcctcat cttctgtaac ttcacgcgca ttgttcattg acataacaac
    11101 attagttgat tgaaaaatca tacttactgc atagataacc ccaataatta gagctagaac
    11161 aagcccaaat tggtagctgc ctaataccaa ataacctaca gctgctccaa taactgctaa
    11221 aagacagaag aagacaatta acaagacaac tgtttttctc ttattacttg caatttgttg
    11281 atacagcaaa agcctctctc ccttctcaat taaaagtcaa aggaaacttt tggcacttct
    11341 ttttcttctt caggagtttc taagaattgg cttggtttga acccaaatag ttttccaaca
    11401 atattacttg ggaaagtttc taatttcaca ttataattgg atgttgttgt gttatataac
    11461 tgacgtgaat aagaaatttt attttccgta ttagttagtt cttcttgaag tttaacaaag
    11521 ctattgttgg cttttaaatc tgggtaattt tcagcaaccg ctaaaatact tgaaagctgt
    11581 cttgttagct cattacttgc tgtcattgct tcagcaggtg tgttagcttt agcaacttga
    11641 gcacgcagtt ctgcaatctt ctcaagtgtt ttcccttcgt acgcagcata gcctttaaca
    11701 gtttcgatta ggtttggaat taaatcgtta cgacgtttca attgaacgtc tatttgactc
    11761 catgattctt tagtatgcat gcgacttcta actaaactgt tataagcaac aatcaaccaa
    11821 ataacaaaca aagcaataat tgctattaaa atcatagttc ccatagtagt agctccttta
    11881 cattttttcc attatagcaa ttttctttag attaggaaag tcctgttatt cccattatta
    11941 gtatttgtga taaaatagag tcattatacg atatagaaat tgaggtttac tatgacaccc
    12001 caagcatttt atcaagttct gatagagcat ggtattacac ttactgacaa acagaaaaaa
    12061 caatttgaaa cttattttag acttcttgtt gaatggaatg agaaaattaa cctaacagct
    12121 attacagata aggaagaggt ttacttaaag cacttctatg attctattgc gccgatttta
    12181 caaggttata ttgacaatag tcctcttagc attttagata ttggtgctgg tgcaggtttt
    12241 cctagtattc ctatgaaaat cctttatcct gaaattgata ttactattat tgattcactg
    12301 aataaacgca ttaattttct aaacatatta gctaatgaac ttgaactttc aggagttcac
    12361 ttcttccatg ggcgcgctga ggactttggt caagataaag tatttcgagc taaatttgat
    12421 atcgtaacag ctcgtgcagt agctagaatg caggttctag cagaattgac aatacctttt
    12481 cttaaagtta atggtcgctt gattgcactg aaagctgcag ctgctgaaga ggaattaatt
    12541 tctgcagaaa aagctttgaa aacactattt tcacaagtaa ctgtaaataa aaattataag
    12601 ttacctaatg gtgatgaccg aaatatcaca atcgttagca agaaaaaaga aacgcctaat
    12661 aaataccctc gtaaagctgg tactccaaac aagaagcctt tgtaaagttg ggagctagta
    12721 tgaaacattt ctttgattac aagacaatgt ctgtcgcacg taaactcagc attagtttta
    12781 tcgctgttat cctattggga agtattttac tttcacttcc aatttttcaa tatgctaacg
    12841 cccctaaaac acattacatt gatcaccttt tcacgactgt ttcaatggta tgtgtaactg
    12901 gtttatctgt ttttcctatc agtaaggttt ataacggttg ggggcagatt gtagctattt
    12961 tactcatgca aacaggggga cttggtttag taacactaat gtcgttgtct tactataccc
    13021 taagacgtaa aatgtcgtta aatgatcaga cccttttgca gtcagcgata acttataaca
    13081 gttcgacaga tttaaaaaaa tatctctata tgatttttaa agtaacttta actttagaag
    13141 ttttagcagc cagcatttta gccatagatt ttattcctcg atttggtttg ggtcatggta
    13201 tctttaatag tatattctta gctgtctcag ccttttgtaa tgccggtttt gataatctag
    13261 gggcaacgag cttagctcaa tttaaactaa atcctttggt taacattatc gtttgtttct
    13321 tgattatttc aggaggactt ggatttgccg tatggaaaga tttaattgaa gctactatac
    13381 aaacatctca caaggaacca aaattaatca agacatttcc aaaacgcctc agcaaccatt
    13441 ctaaactggt tttaaaaaca acaaccatta ttttactaac aggaactttg ctttcttggt
    13501 tgttagagtt tggaaatttt agaacaattg ctaatttatc tctacctaaa caactaatgg
    13561 tcagcttttt ccaaacagtt acgatgcgta ccgctggatt ttcaactatt gattataccc
    13621 aaactgattt cgcaaccaac cttgtttata ttatccaaat gttaattgga ggagctcctg
    13681 gtggaacggc aggaggattt aaagtcactg ttatcgccat tttactctta ctatttaaag
    13741 ccgaactgag tggccaaagc caagtgactt ttcattatcg aacaattcct tccagtatta
    13801 ttaaacaaac actatcgatt ttaactttct tttttatcat acttattagc ggctatctcc
    13861 ttcttctgga actcaatccc catatagatc ctttttctct tttctttgag gcatctagtg
    13921 ccttagcaac cgtgggtgta acaatgaaca ctaccaatca attaactctg ggcggtcgaa
    13981 ttgtaattat gttcctcatg tttattggtc gagtcggtcc aattaccgta ctgctatcaa
    14041 ttcttcagaa aaaagagaag gaaattcact atgctgaaac agaaattatt ctaggttaga
    14101 aaaggtcttt tatgaaaaca aaaattattg gtgtacttgg tctaggaata tttggacaaa
    14161 cactcgcaca agaactaagt aactttgaac aagatgttat tgctattgac agcaatcctg
    14221 aaaatgtaca agctgtcgcc gaagttgtta caaaagcagc tatcggagac attactgatt
    14281 tagctttcct aaaacacatc gggatcagtg actgtgatac tgttattatt gctacaggaa
    14341 acagtttaga gagctcagta ttggccgtaa tgcactgtaa aaagttaggc gtcccacaag
    14401 ttattgctaa agctcgaaac cttgtatacg aagaagtact ttatgaaatt ggtgctgatt
    14461 tggttatctc tccggagcga gaatctgggc aaaatgttgc tgcaaacctc atgagaaata
    14521 aaattacaga tgtcttccag attgaatctg atatttctgt cattgaattt aaaattccca
    14581 aatcatgggt agggaaaact gtcgaacaat taaatatccg tcacaagttt gatcttaacc
    14641 tcataggtat cagaaaagca aaaaacaaac ctgtcgatac cgaagttcca atcaatactc
    14701 cacttgaaga aggtattatt ctagtggcta tcgctaactc agatgccttc caacgctatg
    14761 actatttagg atactttaac taaagaaaat aaaaaactga ctattaaagt cagtttttta
    14821 ttgccatggg ttatagaaac gtccatgatt aaggtgaatt aaataaaggc ttacaaacag
    14881 actagctaga gcaagtatga ttaagacaat gtcacttttt tccaaagatt gcttagagta
    14941 ccaagtacgt cttttatttt taccaaatcg tcgaagttcc atagcagttg ctactgtatc
    15001 aatacgttct agagagctaa aaattaatgg tgtgacaatc tgcaaattcc ccttaatgcg
    15061 tgctactaag ttcgattttt ttgataattc tattcctctt gcctcttgtg ctcgacgtat
    15121 tgtataaaat tcttcttgaa catcaggtat atatctaagg gtcaaactaa cagcataagc
    15181 tattttatat gataaaccta attgatttaa acttgatgcg aactgactag gattagttgt
    15241 catcaaaaac aaaagagcta aaggcactgt acaaaagtat tttaaaatca gattaaagag
    15301 gtaaaacaac tcttggctag tcacatcata tgggccaatt ccttttaaca ataaagaaga
    15361 actaccataa atcttatcac cataatgggg agcaaaaaga tagaccatta taatatttaa
    15421 aacagcgaat acaacaacta aagtcataac aaaggacacc tgttgccatt tgatttttgc
    15481 taatctaaat aagactagcg atcccaatga aacaacaatt aaaaatcttg tatcataagt
    15541 ggtcatgcaa gcaacagaaa ctaaaataaa aaatagtaac ttactggcac ctgatagcct
    15601 gtatagaaag ccataaccct cttgataacc aattaattgt ttagccattc ttacctcctt
    15661 gattatcaat gtaccattgt gttagtttct gtggagatat ccctaatctg tctgctaaag
    15721 caaataaact cgttttcttt aaatgagtct ttttcaaaag ctctgtattt gaaaggatgt
    15781 taataggatg atcatctgca acaatcagac cattgtcgat tacaattgca cgatctgtat
    15841 actctaacat aagctgcata tcatgtgtaa tcataacaat ggtatgtcca tcacaactta
    15901 gtttatcaag gaaagacatc atttcagtat aatgcttcat atcttgacca gctgttggtt
    15961 catccaaaat aataacttct ggatttaaaa tcagtatcga agcaatcgta acacgttttt
    16021 tctgaccgaa tgatagagcc gatataggcc aattcctaaa ttggtagagc ccacaaacct
    16081 ttaatatatc gtagacacga ctttctatat cattatcact aaaacctctt aaacggagac
    16141 ccagagcaac ttcatcaaaa accatcgctt gacttatcat ctgattggga ttttgcaaaa
    16201 catagccaat tcgttctgct ctttctttta tagagtcttg actgacatct tcattattat
    16261 aaattagagt agcattatta gggataaatt gacataacgc tttagctaaa gtagatttac
    16321 ctgcaccatt tttcccaacg atagctaacc gctgaccttt atataaatca aacgaaacat
    16381 catctaaagt attattctct aaatcatatg acaccgacaa atgctgtact gataataatt
    16441 tatctgtatg tttatctaaa tctaatgctc gcattttagg tatcaatagt tcactgaaat
    16501 caaagtcatc aatagacgtc attgtatttt gtttttcaaa gtcatatcct aaatttttga
    16561 gagcagaaat atacagtggt tctcgaatac catattcaga taaaaaatta gtttttaaaa
    16621 gttgatccgg ctccccatta taaataattt gtccatcact aaaaagaact acacgatcta
    16681 ctcgctcata aagaacatct tccaatcgat gttcaataat aatagtggtc gcatcttgct
    16741 cttgatgaat acggtcaacc aatgccatga tatcttgtcc cgattgtgga tctagattag
    16801 ccaaaggctc atcaaataat agaattgggc tatcgtctac taaaactcca gctaaactaa
    16861 cccgttgttt ttgtcctccc gacaagtctt gtggacgttt agacaaaaga ggagctattt
    16921 ctaaacgttt tgcccacatt tcaacgatag aggccatttc ttcctgtgct acaacatcat
    16981 tttctaatgc aaaagcaata tcttctgcta ctgtcaatcc tataaattgg ccatctggat
    17041 cttgtaaaac cgtagaaact aaatgagact tgtcatatat tgacaagtca aaagcatttt
    17101 tatggttaat cgtaaaaata ccactatatt ggcctttatg agtatttggg ataataccat
    17161 ttaaacaatg accaagcgtt gacttccccg accctgaagg tcctaatatc aagacctttt
    17221 cacctttagg aatgcttaag ttaatccctt ttaaagttgg ttcagattga acgtcatatt
    17281 gaaaggtaaa atccttccat tcaataaaat ctttcataga ttactctctt atcctaatct
    17341 ttggataaac tacctttttg tggtcgtgat ttagcgtatg ctagcaacaa aagcgtacca
    17401 cctactccaa tagtaatgga attcacaagg cttgataaga aaccttgtgc aaaaactttg
    17461 cttgcaggtt cgctataaaa gaaaatatca ccatacggtg caacgacaga ccaacctata
    17521 aggttagcaa taacttgaac tacattaaat agaagcaaat ccttcttaat catgccagat
    17581 aattgaatag ctaatttttt agcaaaaaag cctatcatca aacctaaaag accactaact
    17641 aaaacccaag tccaccacgg agaaccatat tgaatcgaat ctttcaatgc gtgtccgata
    17701 aaaccagtaa aaaaaccaac tcctggtcca taaataactg caaataatgc taaaactgca
    17761 tactgcaatt gaatattagt atttggaatt ggtgttggta tattaactag catacctata
    17821 atgataaaaa gtgcagctcc aattccagtc gctacaacct tttttattgt gtttgtattc
    17881 ataaaaacct ctctgaaaaa caagcttaaa cagtcactta caatctgttt tattttatca
    17941 tatttcaagc tgatttgaaa ccttattgac aagaagaact gaaaaacgat tgaaaataat
    18001 attaaatacc aaaaaagatc tcagatagaa tacctaagac cttcattagt ttattttttt
    18061 ccaaaaactg atttttcagc tacagccgtg aacagaacgt ctgtcgatga atttaaagct
    18121 gtctcacacg aatcctgtat cacccctaca atgaaaccaa ctcctacgac ttgcattgca
    18181 acgtcatttg aaataccaaa taagctacat gccaccggaa tcaataaaag tgaaccacca
    18241 gttacaccag aagctccaca tgctgataca gcagcaacca cacttaataa gaatgctgtt
    18301 ggaaaatcaa cagtaattcc taaagtattt actgctgcta aagtcaggat attaattgta
    18361 atagctgcac cagccatatt gatagctgca ccaagcggga tagatactga gtaagtgtct
    18421 ttatctaatc ctaaatcctc acatagtctc atattaacag gaatattcgc cgcagaacta
    18481 cgtgtaaaga aagctgtaat acctgaatcc tttaagcatc gtaataccaa agggtaagga
    18541 tttttacgca tcataacaaa agcaatgatt gggttaacga caagagctac aaataacatc
    18601 gtacccacca aaactaatat taaaagacca taatcaccta gtataccaat accgttttct
    18661 gaaatgctag tgaaaaccaa ccccataata ccaattgggg ccaaattgat aatccatttg
    18721 acaacttgtg aagtagcatc agcaaaagtt tgcattaaat cttttgtacg ctgattactg
    18781 cttcgcatcg ccaaaccaaa aataaccgcc caaaatagaa tactcatata attggcctcg
    18841 gtaatggcat gaatgggatt atcaacaatt ttcaacaaaa gagattgaaa gacttctgaa
    18901 atccccttgg gaggtaataa gtctgtcttt gctggcgttt tcaatatcag tgtcaaaggg
    18961 aaaatatagt tcacaacaac agcgattagg gccgctgcaa aagttgcagt gatatacaaa
    19021 ccaattatcg ttgacatatt ggtcttaccg ccctcacggt gttgcgacaa ggcactaata
    19081 actaaagtga aaactaaaag aggggcaata gctttcaacc ctcctacaaa aagttgacca
    19141 agtaaaccaa tcgcagaggc tttagggact aaaataccca agaataagcc aataacgact
    19201 ccaattgtta ttttttttat caagcttgtt tttctccaag cttttaaaaa tcgtttcata
    19261 ctaaactcct aaaaaattct taaagtctat gatatgataa aaaaaaagat tcgtcaaata
    19321 ccttttttat tttttagagg ttgacgaatt ctaaattttt ctgatataat tactttttga
    19381 aatattcaat caatttcgaa aataaaatat ttttttgtac agaaaggtac attgatgaaa
    19441 ctaaaaaaca cttatatcat tatcggcttg atgctttttg cgctgttctt tggagccggt
    19501 aatcttattt atccagccta cttaggtatc tactctggtt ctaattgggc gtgggctatc
    19561 attgggttct gcttaacagg cgtaacttta cccctacttg gtgttgcagc tgtgacgtac
    19621 tcaggagcaa ccgatgttga aagcttcacc aaacctgtct ctaaaacata tgctattttc
    19681 tttgcagtag cactatacct atctattgga cctttctttg ccattcctcg aacaggggca
    19741 acctcattct cagttggtat tcaacctatc ttaggcgaca gctccttagt caaaattatc
    19801 tatggtttaa ttttctttgg tatttcttat ttcttagcta ttagacctag tcagattgta
    19861 gatcgtattg gtaaatatct aactcccttt ttactaactg ttattagtat tttaattatt
    19921 gcctcactca tccatccagc tgctatcgtt ggaacaccac ataacgcttc aacgaatgtc
    19981 agcgatgctt tcaaggattt accattccta gcaggactga ttcaggggta tggtacaatg
    20041 gatgcccttg catcaattgc ttttgctatt atcgttattc aagcaagtaa acagtacgga
    20101 gcaattacca aaaaagaaat tacttctatg acacttaaat ctggtgctat cgctacattt
    20161 cttttagctt ttatttatat ttttgtagga cgtattggtg caacttcaca aagcttgttt
    20221 aaatttgcaa atggttcctt cttacttcac aatactccaa tagacggtgg gcatgttctt
    20281 agtcaatcag ctaacttcta tttaggaatt gtgggacaag ctattttagg tacggctatt
    20341 tttcttgctt gcttaacaac agctactgga ttaatcactg cttgtgcgga atacttccac
    20401 aaattacttc caaagattag tcacattact tgggctacaa tctttacttt aattgctatt
    20461 acgttctatt ttggtggttt atcagagatt atccgttggt cactaccagt tctctacctt
    20521 ctctatccat taactatagt cttaattttc ttagtctttt ttgatcaaaa atttgaaagt
    20581 agccgcattg tctaccaaac ttctattgcc gccactgctg tcgctgcttt atacgatgcc
    20641 ttatcaaaac tcggcgaaat gactggttta tttaccattc catccgcttt gaccacattc
    20701 tttactaaag ttgttccttt aggtgaatat tctatgggat ggattagttt tgcaatttgt
    20761 ggtgtcttag ttggtcttat tctaaaaaaa gtaaaagcat aataatttaa agcagtacga
    20821 aaattactgc ttttatttta aaataactat tgtaaccgct ttcttttggt ggtataatag
    20881 ccttgaaaat aaataatttt tggaggaact ctaatatgag agctgcaact tatctttcag
    20941 ctaatgaatt atcacttact gataaggcta aacctcaagt aataaaaccc acagatgcgg
    21001 ttgtaagact tgtcaaaaca accatttgtg gtactgatct acatatcctt ggtggcgatg
    21061 ttcctgcttg taaaccagga acaactttag gtcatgaagg cattggtatc gtcgaagaag
    21121 ttggttctgc tgtcaatcat tttaaagtcg gagataaagt aattatttct tgtgtcactg
    21181 cctgtaatac ttgctattac tgtaagcacc agttgccttc acattgtgaa gatggcgggt
    21241 ggattttagg acatcttatt gaaggtactc aagcagaata tgttcatatt ccacatgcag
    21301 atggtagcct ttatcatgca cctgaaggtg tttgtgacga tgcattggtt atgttatctg
    21361 atattttacc aacttcatac gaaatcggcg tcttaccttc tcacattaaa ccaggtgata
    21421 ctgtttgtat tgtaggcgct ggaccaattg gactatctgc cttactaact gcacaattct
    21481 attctcctgc caaaattatt atggttgatt tatctcaaaa acgacttgaa gcctctaaaa
    21541 aatttggtgc aacacacacc attctatcaa cgtctactca agaagttaaa gaagaaattg
    21601 ataaaattac caaaggccgt ggcgttgatg tcgtattaga atgtgttggt tatcctgcaa
    21661 catttgatat ttgtcaaaat gtagtttcta ttggaggaca tatcgcaaat gttggtgttc
    21721 atggtaaacc tgttgaattt aacctccaag atttatggat aaaaaatata acccttaata
    21781 ctggattagt taatgctaat acaactgaaa tgctccttga agttcttgag acaggcaaaa
    21841 tcgatgcaac acaactagta acacatcact tcaagctatc tgaaatcgaa gaagcttata
    21901 aggtatttaa agctgctgaa gaaaacaata ctctcaaagt tatcatcgaa aacgatatta
    21961 ctccagcctg ataattttta aacaaatgaa aatgtgagaa gaatagcctt ctcacatttt
    22021 tatttatgac tcaagcgacg tgttaaaaaa tcacctaaaa attggataaa gaaaattaat
    22081 aataaaatca gaatagtcgc tactaaagta atatcatcac gagaatagtt atatccttta
    22141 gtaatagcaa cagaacccaa tcctcctgca ccaatagcgc cagccatagc tgtttcacct
    22201 actaaagaaa tcaaagtaac cgttgatact cgaattaaat ctggtagacc ttcacgaaga
    22261 taaactacaa taatatccca aagtgttcca cctgaggctt gtgcagcctc aataactcca
    22321 ccatcaagtt cagctaaaac aacttgaact tgacgagcaa aaaatgggaa aactgccaaa
    22381 gaaagaggta ccaaagctgc tggtgaacca agtgttgttc ctacaattac gcgagttact
    22441 ggcgcaatca aagcaagaag aataatgaag ggcagagctc taaaaacaga aacaacttta
    22501 tctaaaactc caaatactaa tttattagca ataactcctc taggactagt taaaacaagg
    22561 aataaacctc ctaacaatcc cattaaacct ccaataagga acgaccaaaa agtcatataa
    22621 agggtttgta caatagctgt ctgccagccg taagcacctt cccaacccat ttgatataca
    22681 tttggtaaat gtgtttgaat ccactctatc atgctttacc accttctttc aataacgtta
    22741 attgaacttg cgaatctgta attgcttggc aggcttgtct taatttttct tcgtcacctg
    22801 ataagataac taccatttct ccaactggaa taccatctaa aatttcaata ttcccataga
    22861 ggatgttagc agttacttca aattctttat aaatttgatt taaaagtggc tcatccgtgc
    22921 tatgcccagc gtatttcagt tgaactaatt tgctacctac tggtagattt ttgacaactt
    22981 cttgttgctc aatcttaagc attgcctcat caatcccagt agctattttt ataaaatctt
    23041 gtgtaagact ctctctagga tgtgaaaaaa tgtctaaaac tgatccttct tcaatcaatt
    23101 taccattttg cataacagca acgcgattag caatgtcttt gacaatttgc atctcatgtg
    23161 taatcaaaac aatcgtaagt cccaattttt tattcagatc ttggagaagg gcaagtattt
    23221 gttttgttgt tttagggtca agtgcagagg tagactcatc tgaaattaag attttaggat
    23281 cgttagccaa tgcacgcgca atggcaacac gctgtttttg ccctcctgat aattgtgaag
    23341 gatagttttg agcacgatcg gataatccaa caagttctaa taatttagca accttagctg
    23401 ctttcgcctc ttttgataaa cctgaatgtt taagagcaaa agctacattc tgttctgctg
    23461 tcaactgtga cattaggtta aaatgttgaa aaatcattcc aatttcacgg cgctgttcgc
    23521 gtaattgtgt gctagtcagt gtcaccttgt tgtcgtaaat aacttggtca tcaatagtaa
    23581 ttttacctgc tgaaggttct tgtagcaaat taataacacg aacaagggta gattttccgg
    23641 ctcctgaata accgacaatt ccatatatat caccttgatt aatatggata gtaacatctt
    23701 taacagcatt aatctcacgt ttcttttggt ggaaagtaac atcaatgttg tctaatttaa
    23761 taattggttc tttactcata tgattctatt aaagcctcca ctaattcaat atgggtgtaa
    23821 taatcagcaa tactaacatt ttcatcaacg ccatggtctc gactattggc atgtcctatg
    23881 ccaatagctg caattggcgc tcttagagct tgatgaacta aatacattgg tcctgtacct
    23941 ggtgaggtag gtaacagaga tatcccttct ggataaaatt gctcagctaa atcaacaacc
    24001 tttaggatag atggcgctga catatcacta cgataagaca tttcaccaag ggtataggtt
    24061 agctcaatgt ctttaaagcc attctctttc aaatgatttt gtattgaatc tagtacacct
    24121 ttcggggtta accctggtac taacctaacc tctgctttac atttagcata agcaggtaaa
    24181 attgttttga cgccttcacc ttgataacct gaggttatac cttcaatggc tatagaaggt
    24241 tcaaaataaa gctttctcaa aaaagtttta tgactatctg ctaaactggg caaaactaat
    24301 tggtaagcac cttctagcgc ctttgcagat ctataagaat atttttcaac taattctaac
    24361 tctctcttag tagggggaat aactttatca taaatacctt ctaccaatat atgacctttg
    24421 ttatccctta acgtattgag ggcttgaatt aaataccatg tcgaggaatc aacaacccca
    24481 ccaaaggatg aatgaatatc aacatccgct gaacttactg ataaatcaaa agtgacaatg
    24541 cctttatttc caccagcaat ttccaactgt cctttaggat ttttagggcc atcttcccac
    24601 acgatcaaat cagccccttg tagctgctct tgatactttt ctaaataata atcaaggcct
    24661 actgaagctg attcctctgc tccttccaca ataaaagtga tatctagagg aagttcccct
    24721 ttgtgtcttg ataaatattt tttaactgct gataaacgtg cggtaatgtg acctttatca
    24781 tcatctacac cacgtccata catcttacca tatcgaagtg aaagtgtaaa agggtcctca
    24841 gtccactgtt ctacctcatc tgctggtaca gtatcataat gattgtaaaa aataatacgc
    24901 ttagcatcta cttttgatga tttgaaattt gccacgataa agggagcgct atagctgtca
    24961 tctataatga cttctgcgcc agcttttctg aaaacttctc caagatagct tgataagtca
    25021 tttaaaccaa ctttctgagc aaaaattgac ttaattgcaa ttaaatctct taaaatattg
    25081 atatcatcct gaacaatagt atcagataga aacttttgaa tttgattttc ttcgtttgtt
    25141 aaagtcatct ttcatccacc ttccttcaaa aacaatccaa gagcctggca cattgtattc
    25201 gtcccaggcc ctttatggct atatggctga taagttgtat cctattagga ttccctgtta
    25261 aacttataca gtcaattcac gttaccattg tggaatatca gctgaagtat ctttgataac
    25321 ttttttcact tcatctgtgt gataagcatc caagatagct tggatagctt tagcgttctt
    25381 ttgctttttc caatttttac gtcccgcaat gatattaatc cattgttttg aatttttatc
    25441 tgatttctca acaaagatag catctgaagg ttttaaatta gcttgctcaa tgtatgtatt
    25501 attaataata gctgcatcta catctttgag tgcacgtggt gtttgactcg catctaactc
    25561 ctgaatatta atatcctttt tattagatgt gatattagca actgttgcaa ccttcttacc
    25621 agaaacattc aatttgatta aacctgctga ctgaaggaca tacaatgcac ggctaccatt
    25681 tgttgcatca tttggaattg caatagtggc tccttttttc aattttttaa gagattttac
    25741 cttctcagaa tagatacgaa ttggagctaa gtaagtcttt tcaagtggaa ttaagttttt
    25801 cttattttcc ttattccagt tttctaagaa attgtaatgt tgaaaggcat taatatccac
    25861 atccttattg gctgtcgctt gatttggttg tgtataatct gtaaattctg taaatttgat
    25921 tttagcttta tcacctacta gcttttcaat tttatcccaa cgtgcttttt cagtgtcaga
    25981 aaaggtcata acaccaactt taacaacttt acttgaagtt gagcttgctg aaacttcttg
    26041 attactaaat gtgactccta aaagtgttgt taaggctaat cctaagacaa tatgttttaa
    26101 cttcataata aattccctca atttttgtga taaagatatt atcccatagg aagttattag
    26161 aatcaaattg ttataaactg tctcttgtta tagttttaaa ctatataaca aaaaactaag
    26221 gattgacatc tccttagttt ttattactac ttctccttag gagtaacgag atctttctca
    26281 tttggtacgt aatcacctcc aaggtattct ttagctattt ttgaaagtgt cccatcttta
    26341 cgtaattgtt taaggcgatt attcacatac ttttgcaagt ctttttgatc ctgaccaaag
    26401 atgaaataaa tataaggctg atctctcata gtcaaaggga tggtttttaa attcgtgaga
    26461 ccttgattct ttatgatagc gttaacagtt ggtccgtcaa aaagtttaaa atcagctttt
    26521 ccatcactca aattcgttag aatctgtgta atattttcat tagtatattt tagagtaaca
    26581 ggatttttct gatgctcttt attgaaattt tctaattgct tggcagttgt cgttccttgg
    26641 acaacctgtg ttttatgacc actaatatca ttataagctt tgatattact attcttagga
    26701 actgctaaaa ctgaaggagt agagcctatt gggtatgaaa ataaatattt ttgagatctc
    26761 tctgatgaat aagaaatatt atttccaccc atttgaaact ttcctgaatc aatgccggta
    26821 aataccgatg accattctgt ttttttaaaa gtgactttat agttatctga acctttaaaa
    26881 acagctttgg ctacttcaat atcaaagcca gtcagtttcc catccttcac ataagaaaaa
    26941 ggtgccgttg ttccaacagt tgcaaaatta acttcttttt tgcccgtctt agtagtatga
    27001 gattttgagc tacaagcagc tagagacgct actgtcacta aaataattcc tgttacactt
    27061 aacacttttt ttattgtcat cttttttctc ctctttaatt gtacacgaca tcataacatc
    27121 tttaagtttt taactaatac ttatcttcta tcgtctgaga gtttttgcta ttatatggat
    27181 gctacgtttc attaacaaaa taaccaaata aggtttggtt ttcaatttgg tgaagcatta
    27241 attctggatg ccattgaaca cctataatac attgttctgt tcgactttca taagcttcta
    27301 ctgtcccatc tctaggatct ctagcactaa ctatcagacc ttcagctaac agtttaacac
    27361 tttgtctatg aagagaattg atcttaaaat cacctgatat aaagccttct agaaaactcc
    27421 ctttttccac atttaaataa tgagcaaatc caatgtaagg ctcttgatag tgattatcaa
    27481 tagattgatt aagtgtacca ccaagcgcca catttactaa ctgcatgcca cggcaaatcg
    27541 caaagattgg tttattttgt ttcaacgctt cttcgactaa tgcaaattca aaaatatcac
    27601 gcgcaagtga ataatcatca ctctcaatta tcttctcttc accataatag gatggtaaaa
    27661 cattttggcc accagatatg atcagcttat ctatcatctc aacataagct ttggcacttt
    27721 ctgcttcact tattggtagg ataactggta acccgcctgc gtttttaact ccttccgaaa
    27781 tatgcctaga taccgaatca taataataac ctggaatatc tgacatttct ctttcatttc
    27841 ctgtaatacc aataataggc tttgttaaca ttttgctccc tcatcaacca ataactgtct
    27901 aataattatc attttacacc taaaaaaact ttttgaataa tattttttac ctatcacttc
    27961 tttaacaata cctatcaact ctcacaaaaa taaaaaccat agtattaata ctacggtttt
    28021 attttttaga aatctgctac attatgataa actttttgaa catcatcatc tgattcgagt
    28081 gcgtcaataa gcttttcaaa agtttcaagg tcatcacctt ccaatactac ttctgattga
    28141 ggaatcattt caagttcagt aacttggaat tcttctacac cattatcgcg aagtgcttgg
    28201 atacctttat gaagatctgt tggggcggta taaactgtta ttgttccctc ttctgcttca
    28261 acatcatcta cgtctacatc cgcttcaagt aattgttcga agacagtgtc agcatcatca
    28321 ccagcaaaaa cgatgacacc ttttttatca aataagtagg ataccgatcc tgaagctccc
    28381 atattgccac cgttcttacc gtaagcagta cgtacatttg ctgccgtacg gttaacattt
    28441 gatgtcaaag tatccacaat aatcattgaa ccatttggac caaaaccttc atagcgtccc
    28501 tctacgaaag tttcatctgt gtttcctttg gctttatcaa tcgctttatc aataacatgc
    28561 tttggaactt gtgcttgctt agcacggtcc aaaacgaatt ttagagctga gtttgactcg
    28621 gggtctggtt caccttgctt tgcagcaaca tatatttcaa caccgaattt agcgtatact
    28681 tttgagttag caccatcttt agcagtcttt ttggcaacaa tattggccca cttacgtccc
    28741 attggaaaat ctcctttgat ttcattttta ataacctgct ggctcatatt ggcaagcaat
    28801 cttaatttta actctattat tatagcactt tgatagcaat tggtaaaatg gaaattctat
    28861 cacggtatgc agtagaattt tttagaattg tcaattttct attaaggaga acctaccgag
    28921 ttccttttat aaaaagactg aacaaatttt cctttgccca gccttgacag attcggtgtt
    28981 atcctgtatc ttacgataaa gactaagtaa gggaacgttg taattggact tactcggtaa
    29041 agcgtagtgg ttaccataac tgcgcttctt ttattcgatt aattcaatag aattgtagca
    29101 cactacaaaa cgaataacga gctttttaaa gaaaaatata agaaaagccc ttttaaaggt
    29161 gcttttctta tatttgtttc aaatcaatca atttctttat ttctttaatt tcgacatcct
    29221 taaaaggaac cgttttttca aactcgcctc tattaacttg tttaaatcct agatctctgt
    29281 cattaccaat ttgtacatca atagtgcata actcaactat tttctctcta ttttttcctt
    29341 ctctaataaa aaattgtgta aatatatggt cacaatagaa gcaacatctt tccacttgat
    29401 acacctcact aatttcagtt tgatcaaccg gtcgtatgtg ataatattga tatttgctga
    29461 aacccttttg ccactttcta ctccatagga aaaatttatc ctgcttacta tataacgaca
    29521 cctcctttcc ttggaaaact gtctctccca catgagtcgc accatcaagt ggtgcatctt
    29581 tctcatcaaa cgaaatcttt ttcttagaca aaatcgacat tatcaactgt gcagaaccta
    29641 ttgcataaaa aattgttcct acaataaata ctagtaaaat tattgttagt acaatgattc
    29701 caaaaacttt ctgtgcatcc attgaggaat acccagacaa attatatacc aatgtatatc
    29761 cacctacagc agttattgat aggcaaataa aaaataatat caacacaccg tacgaagaat
    29821 ccatgatctt acatactaac ttttctgata gaaatttcca tacaaggcgc acaacacttc
    29881 cctccctaaa tat
//
LOCUS       DATRQA010000007        28468 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.007, whole genome shotgun sequence.
ACCESSION   DATRQA010000007 DATRQA010000000
VERSION     DATRQA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28468
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.007"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            85..870
                     /locus_tag="VBD27_000239"
     CDS             85..870
                     /locus_tag="VBD27_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7024281.1"
                     /translation="MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSV
                     DTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLNADVNVKAYVQNTIDNQQRLSTA
                     NAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
                     WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDT
                     DLVPFAMHLEAKSQDGTLEFNVAIPNTQASYTMDYATGEITLN"
     gene            complement(948..1586)
                     /locus_tag="VBD27_000240"
     CDS             complement(948..1586)
                     /locus_tag="VBD27_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEO7024282.1"
                     /translation="MFLIDFILHIDTHIYAMANTVGNWTYLLLFLVIFVETGAVIFPF
                     LPGDSLLFAAGALAANPKMSFNIVTFLIIFFIAAFIGDSCNFLIGRTFGYRFIKHPFF
                     RRFIKEKNIRDAELYFEKKGTAAIILGRYIPIIRTFVPFVAGISQLPPKVFIKRAFIA
                     ALSWSVIATGSGFLFGNIPFVKQHFSLIILGIVFVTLIPVLISGVKSYRNTK"
     gene            1803..2459
                     /locus_tag="VBD27_000241"
     CDS             1803..2459
                     /locus_tag="VBD27_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7024283.1"
                     /translation="MSYLTFKHVDFKTDDKLVLNDINFAIDEGDFVSIVGPSGSGKST
                     VLKLASGLMSPTAGHIFFDGKDLNQLEPIESRKMISYCFQTPHLFGNTVEDNISFPYH
                     IRHEKVDYRRVDDLFQRFEMDQSYLKQDVKKLSGGEKQRIALIRQLLFEPKVLLLDEV
                     TSALDNHNKAIVEKVIKSLHDKGITILWITHDEEQSRRFANKVLKVVNGSIEAMEVIK
                     "
     gene            2456..3229
                     /gene="fetB"
                     /locus_tag="VBD27_000242"
     CDS             2456..3229
                     /gene="fetB"
                     /locus_tag="VBD27_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEO7024284.1"
                     /translation="MSGANSISLMSLLMASSLVLITLFFSYWQKLNLEKEVIISAIRA
                     VIQLLAVGFLLDYIFGYQNPIFTALLMLFMIINASYNAAKRGKGINKGFVISFIAIGS
                     GTIITLSVLIFSGILKFVPNQMIPVGGMIISNSMVAIGLCYKQLLSEFRSKQEEVETK
                     LALGADILPASIDIIRDVIKTGMVPTIDSAKTLGIVSLPGMMTGLILAGTSPIQAVKY
                     QMMVTFMLLATTSIASFVATYLAYKIFFNNRKQLVVTKD"
     gene            3393..4211
                     /locus_tag="VBD27_000243"
     CDS             3393..4211
                     /locus_tag="VBD27_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEO7024285.1"
                     /translation="MILKICRAAYSLQWGGVYQLALLDYPRIKAFELERIGAFIAYEK
                     QYKRKTEIQCDDKHLLAKIVHFLKYNSFTFPYIPKYREAAATFNEDGISLTSDFLSHT
                     CTIETAKLIFKEGKILSAVKAFNKPAEVLVKDKRNAAGDPKDYFDYVMLNWSNTNSGY
                     RLVMERLLGKAPSEQELTVGFKPGVSFHFTYQDIINHPDSIFDGYHPAKIKNQLSLAE
                     HLVACVIPKHYQEDYQSLVPNDLKHRVYYLDYCNETLYEWNQKVYDFLCHLENK"
     gene            complement(4248..5132)
                     /locus_tag="VBD27_000244"
     CDS             complement(4248..5132)
                     /locus_tag="VBD27_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7024286.1"
                     /translation="MDIRQLTYFIAVAEAKNYSRAAKSLFVTQPTLSQSIKKLEAELN
                     TILFLQNGRQLALTEAGEILYEKGQLLMTNVNQMVTEIQQLNQEKKEGIRVGLTSLFA
                     IQFMKQISTFMATHSNVEVSLIQDGSRKLQELLAKGKIDIGLLSFPSTRNDITIEPLQ
                     TSTKGYKVSIVMPKSHPLATLPEIELNDLRDYKVASLNEHYMLGEMLPRKCRSLGFDP
                     HIVFKHNDWEVLIHSLQDLNAVTILPSEFESISQVQDLCWVPLKDKNNFYPIGIAYRN
                     DTSFSPMIEEFLSLLKTN"
     gene            5325..6383
                     /locus_tag="VBD27_000245"
     CDS             5325..6383
                     /locus_tag="VBD27_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO7024287.1"
                     /translation="MSDVVEKQTTKSFIMNVLNGLALGTVIVLIPGAILGELMKALLP
                     MWSGFATLIAATAVATSMMGLVIGIMVGLNFKFNPIQSASLGLAVMFAGGAATFLKGA
                     IMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHVLLPYVK
                     MITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANL
                     GICAASFGLCMAGWSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILG
                     ALFNIQGTPASAGFGISGLIGPINALNLAKGGWSVMNMLLIIIIFVAAPIILNFIFNY
                     LFIKVLKIIDPMDYKLDI"
     gene            6397..7389
                     /locus_tag="VBD27_000246"
     CDS             6397..7389
                     /locus_tag="VBD27_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEO7024288.1"
                     /translation="MKLKVFNVREEEATLAQDWANRNHVELSMSEGPLTLETVNEVEG
                     FDGIANAQIEPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLELAKQHGIIISNVPSYSP
                     ESIAEFTVTIALNLIRKVELIRANVREQNFSWTLPIRGRVLGNMTVAIIGTGRIGLAT
                     AKIFKGFGCRVIGYDIYHNPMADGILEYVNSVEEAVEEADLVSLHMPPTAENTHLFNL
                     DMFKQFKKGAILMNMARGALVETKDLLEALDQGLLEGAGIDTYEFEGPYIPKNCQGQD
                     ISDKDFLRLINHPKVIYTPHAAYYTDEAVKNLVEGALNACVEVIETGTTTTKVN"
     gene            7596..9146
                     /locus_tag="VBD27_000247"
     CDS             7596..9146
                     /locus_tag="VBD27_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEO7024289.1"
                     /translation="MEKTMDMEQREEVVSYNRAKVWQLVMFAMNNASTNIYLFTFMFV
                     TYFSTGVLGSAAIFVSQIMGYIRIFDGFIDPAIGIMIDKTDTKFGKYRPILIIGNVIT
                     ALSLIFLLALRGVDENIRFPLFILVLIIHKIGYSMQQTITKAGQTALTNDPKQRPIFN
                     IVDAVMTTSLMTGGQFVVSVFLVPKFGNFTPQFFNVLIFGTILISAILAIVAIIGIWA
                     KDRKEFFGLGENTQKTALKDYWKVLKGNKPLQILSIAAALVKFAIQFFGDSVVMVLLF
                     GILFGNYALSGQFSLLFIVPGVIINILFSTIARKKGLRFSYVRAIQIGMIGLLAFGAV
                     LYVGKPGDLSLTSLNLYTILFIVTNIIARYASQAPASLVLTMGADISDYETSESGRYV
                     SGMIGTIFSLTDSIASSFAPMVVGFVLAGIGFSKSFPTIETPLPPDLKMAAISILVAI
                     PFIALSIALLLMKFYKLDKEEMVRIQEKIQVMKAATTKERAKDIARNVPLTDMDYVDV
                     TKYPIDKD"
     gene            9213..10238
                     /locus_tag="VBD27_000248"
     CDS             9213..10238
                     /locus_tag="VBD27_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO7024290.1"
                     /translation="MAKIISLGEVLLRLSPPQYHTLMQANHLKCQFGGSELNVLASLA
                     QLGYHVGLVSALPDNDLGKMASQFILSQQISPAAIIKKEGRLGIYYYEQGFSVRTNKV
                     IYDRNYSSFWESTLSDYDFTSIFKGVDWFHVSGITPALTKDLYEVTRFLMTKAKEGGV
                     KVSIDLNFRESLWSSFQEAREQLSPLLGLLDVCFGLEPIYLAGESEDLKDELGLSRPY
                     LDIELLEKITQKIVQEYGLDYIAFTQREMEYTNQYMLKSYLYHNNMLYQTDKTGVEVL
                     DRVGTGDAFAAGLIHALLEKETPQRALEIAMATFKYKHTIQGDINIMTRDDIAYLIEK
                     ETNDIKR"
     gene            10255..12054
                     /gene="uidA"
                     /locus_tag="VBD27_000249"
     CDS             10255..12054
                     /gene="uidA"
                     /locus_tag="VBD27_000249"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEO7024291.1"
                     /translation="MLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSF
                     NDLMVSKEKRDYIGDFWYEKVIEVPKVSEDEEMVLRFGSVTHQAKIYVDGVLVGEHKG
                     GFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQEDGSIKKKVRENFD
                     FFNYAGVHRPLKLMIRPKNHIFDITITSRLSDDLQSADLHFLVETNQKVDEVRISVFD
                     EDNKLVGETKDSRLFLSDVHLWEVLNAYLYTARVEIFVDNQLQDVYEENFGLREIEVT
                     NGQFLLNRKPIYFKGFGKHEDTFINGRGLNEAANLMDLNLLKDMGANSFRTSHYPYSE
                     EMMRLADRMGVLVIDEVPAVGLFQNFNASLDLSPKDNGTWNLMQTKAAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRD
                     QVMDLVDVVCLNRYYGWYVDHGDLTNAEVGIRKELLEWQDKFPDKPIIITEYGADTLP
                     GLHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFADFETNLMILRVQGNHKG
                     LFSRNRQPKQVVKEFKKRWMTIPHYHNKKNSVK"
     gene            12083..12754
                     /locus_tag="VBD27_000250"
     CDS             12083..12754
                     /locus_tag="VBD27_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEO7024292.1"
                     /translation="MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRST
                     IREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLIKDTDRLTSDLFELRLLLE
                     PRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYSGNIAMD
                     SLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRR
                     SALKA"
     gene            12871..13488
                     /gene="eda"
                     /locus_tag="VBD27_000251"
     CDS             12871..13488
                     /gene="eda"
                     /locus_tag="VBD27_000251"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO7024293.1"
                     /translation="MLNQLKNNYFFAVIRGKSSEDALEIAKHAILGGIRNIEVTFSTP
                     EASKVIKQLSDDFKNNKEIIVGAGTVMTTELAKEAIDAGAKFLVSPHFDSDIANLANE
                     NKVYYFPGCATATEIVVARKYKCQIIKLFPGGVVGSGFIKDIHGPIPDVDLMPSGGVS
                     VSNVVEWRKAGAVAVGVGSALSSKVATEGYDSVTKIAKQFVSALD"
     gene            13505..14905
                     /gene="uxaC"
                     /locus_tag="VBD27_000252"
     CDS             13505..14905
                     /gene="uxaC"
                     /locus_tag="VBD27_000252"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEO7024294.1"
                     /translation="MAFNTETFMLKNQAAIQLYEEVKRQPIFDYHCHLDPKDIFEDHI
                     FDNIVDLWLGGDHYKWRLMRANGISEAEITGPASNLEKFKAFARTLERAYGNPVYHWS
                     AMELKNIFGVNEILTESNAEEIYHRLNHFLKEHKISPRRLIADSKVMFIGTTDHPLDT
                     LEWHKKLAADESFKTVVAPTFRPDEAFIEHRHFVDFITKLGDITQKEITDFSTFIAAM
                     EERIAYFAQNGCRASDISFTEIVFEQTDELELNDLFNKVCEGYIPNQSEISKWQTAVF
                     MELCRLYKKYGFVTQVHFGALRNNHSTIFEKLGADVGVDSLGDQVALTVNMNRLLDSL
                     VKKDSLPKMIWYNLNPAYNIAVANTLANFQANELGVRSYLQFGAGWWFADTKLGMISQ
                     MNALAEQGMLANFIGMLTDSRSFLSYQRHDYFRRILCTYLGEWIEEGEVPEDYQALGS
                     MAKDIAYQNAVNYFKN"
     gene            14923..15969
                     /gene="uxuA"
                     /locus_tag="VBD27_000253"
     CDS             14923..15969
                     /gene="uxuA"
                     /locus_tag="VBD27_000253"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO7024295.1"
                     /translation="MEMSFRWYGEDDPVTLENIGQIPTMKGIVTAIYDVPVGEVWSRE
                     RIQQLKEKVEAAGLKISVIESVPVHEDIKLGRPTRDLLIDNYIQTVKNLAAEGIDTIC
                     YNFMPVFDWTRTDLAYQYPDGSTALIFDETVSKKMDPVNGELSLPGWDASYSKEEMKA
                     IMDAYAEIDEEKLWENLTYFIKRIIPEAEAVGVKMAIHPDDPPYSIFGLPRIITGLEA
                     IERFVKLYDSKSNGITLCVGSYASDPQNDVLEISRRAFELDRVNFVHARNIKLGDGKS
                     FKESAHPSEYGSIDMYEVIKLCHEFGFEGAIRPDHGRMIWGETGRPGYGLYDRALGAT
                     YVSGLYEAVIKGSK"
     gene            16093..16932
                     /locus_tag="VBD27_000254"
     CDS             16093..16932
                     /locus_tag="VBD27_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO7024296.1"
                     /translation="MSKTITFTNKVVVITGAGGVLCGYMAKEFAKAGAKVALLDLNQE
                     AAQTFADEIVEEGGIAKAYKANVLSKENLEEVHQAVLEDLGPTDILVNGAGGNNPKAT
                     TDNEFHELDLPSETKTFFELDEAGISFVFNLNYLGTLLPTQVFAQDMVGREGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRSLLF
                     TEDGQPTARAEKILNNTPMGRFGEASELIGGLFFLADEKSSSFVNGVVLPIDGGFAAY
                     SGV"
     gene            17085..17897
                     /locus_tag="VBD27_000255"
     CDS             17085..17897
                     /locus_tag="VBD27_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7024297.1"
                     /translation="MVDTNHKIAFCIDSDGCAMDTMTYKHQLFFGPIAADIFGISEQE
                     RTSFLKEWDRVNLYSRTRGVNRFVGLVMGLEFAGVEHIDKLKEWVETTDSLSNNSLEL
                     ALANDFSRDLEKALEWSNEVNRQIKNYEGDSLAFSGVLESLERLSQFGNVYVVSSANK
                     EAVWQEWEEQGLLKYVDDIYCQDRGKKEDVIATLIASGYCRETILMVGDSLGDLAAAE
                     QNGIAFYPVLVGKEVKSWEILREDIGEAFAKGQFEQQRQKESINTFWANLDN"
     gene            17913..19703
                     /locus_tag="VBD27_000256"
     CDS             17913..19703
                     /locus_tag="VBD27_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEO7024298.1"
                     /translation="MTHLVDLTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGA
                     SRSEEYLTDVLDRYHIAAVRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAV
                     TDGTKVGDEIKVAATNDPKYAYEMGRIAGMEASAVGCNASFSPIVDLTRNWRNPIIAS
                     RNWGANVDQIISLSKEYMKGIMQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWM
                     STFGRIYGELADAGLPGVMAGHIHLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVGMTASMPRRDLLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKG
                     ILTEERLHDALRRTLGLKAKLGLHNYEGRRQELFMPKDKAMALINTLESQKIADEVAD
                     KAVTLVKDKQKDIFPVNPERYRHILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEAR
                     GHEVTVWESTEERIMKLPQEERAAAIANVYAQKQPIANLTEHYDLIINLVDVNAGGTT
                     QRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFALADMPQVGTYINAYDGLPSTISAVV
                     AKLAGESEFTGVSPVDAYCGLIDTHLWRQC"
     gene            complement(19761..20846)
                     /locus_tag="VBD27_000257"
     CDS             complement(19761..20846)
                     /locus_tag="VBD27_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO7024299.1"
                     /translation="MSKLNRIRHHLHSVQAELAVFSDPVTVNYLTGFFCDPHERQMFL
                     FVYEDRDPILFVPALEVSRAKQSVPFPVFGYIDSENPWQKIASNLPSFSVSKVLAEFD
                     NLNVTKFQGLQTVFDGHFENLTPYIQNMRLIKSRDEIEKMLVAGEFADKAVQVGFDNI
                     SLNNTETDIIAQIEFEMKKQGINKMSFDTMVLTGNNAANPHGIPGTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYHLCLEAHQAAIDFIKPGVLASEVDAAARN
                     VIEKAGYGQYFNHRLGHGLGMDVHEFPSIMAGNDMEIQEGMCFSVEPGIYIPDKVGVR
                     IEDCGYVTKTGFEVFTKTPKELLYFEG"
     gene            21056..22060
                     /gene="ccpA"
                     /locus_tag="VBD27_000258"
     CDS             21056..22060
                     /gene="ccpA"
                     /locus_tag="VBD27_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEO7024300.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILARGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPIVLAGTVDLEHQLPS
                     VNIDYKAAAVDVIDILAGNHKDIAFVSGPLIDDINGKVRLAGYKEGLKKNGLNFKEGL
                     VFEANYRYAEGFALAQRVINAGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPIAQYTRPNLTSISQPVYDLGAVSMRMLTKIMHKEELEEKEIVLNHGIVKRGTTNN
                     "
     gene            22192..23658
                     /locus_tag="VBD27_000259"
     CDS             22192..23658
                     /locus_tag="VBD27_000259"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO7024301.1"
                     /translation="MTNELIMQAFEWYLPSDGNHWKKLEESISDLKKLGISKIWLPPA
                     FKGTSSDDVGYGVYDLFDLGEFDQNGTIRTKYGRKEEYLKLIKSLKANGIKPFADIVL
                     NHKANGDHKEKFQVIKVNPENRQEALSEPYEIEGWTGFDFPGRQGEYNDFKWHWYHFT
                     GLDYDAKNNETDIFMIVGDNKGWADDDLIDDENGNFDYLMYNDIDFKHPEVIKNLQDW
                     AKWFIETTGIEGFRLDAVKHIDSYFIQTFINDIRTKIKPDLEVFGEYWKSDQTSMKDY
                     LEATQFQFSLVDVTLHMNFFDASHQNRDFDMRTIFDDSLVIDNPEYAVTFVENHDTQS
                     GQALESRVEDWFKPLAYGLILLRQQGTPCLFYGDYYGIQGEFGQPSFKEVIDKMAELR
                     QNYVFGEQVDYFTHSNCIGWTCLGDEEHNSCLAVVLTNGDQGWKHMEVGEIYAGKTFV
                     DYLGNCEQEVVIGDDGWGDFLVASASISAWVPKIEEAN"
     gene            23703..24701
                     /locus_tag="VBD27_000260"
     CDS             23703..24701
                     /locus_tag="VBD27_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7024302.1"
                     /translation="MKVLLYLEAEEYLKKSGIGRAIKHQEKALQIAGIDYTTNPTDDF
                     DLVHMNTYGIRSWLLMSKAKKTGKKVIMHGHSTEEDFRNSFIGSNLVSPLFKWYLCRF
                     YQKADAIITPTDYSKQLIKAYGIKKPIFVLSNGIDLSRYQRSEKKEAAFRHYFHLSKD
                     DKVVMGAGLYFMRKGIDQFVEVAAKMPDIRFIWFGETNKWVIPRKVRQIVTKQHPSNV
                     TFAGYIKGDVYEGAMSASDAFFFPSREETEGIVVLEALASHQHVVLRDIPVYHGWVTE
                     DSVELATDVDGFVEKLDKVLSGKSDKIKEGYRVAESRSIERIAHELASVYQKVMEL"
     gene            24703..26037
                     /locus_tag="VBD27_000261"
     CDS             24703..26037
                     /locus_tag="VBD27_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO7024303.1"
                     /translation="MRIGLFTDTYFPQVSGVSTSIRTLKEGLEKEGHEVYIFTTTDRN
                     VKRFEDPTIIRLPSVPFISFTDRRVVYRGLISAYRIAKDYELDIIHTQTEFSLGLLGK
                     LVAKALRIPVVHTYHTQYEDYVGYIAKGKLIKPSMVKYIMRTYLSDLDGVICPSRIVL
                     NLLDGYGVKIPKQVIPTGIPVENYRREDISEETIKNLRTELGLADNDTMLLSLSRVSF
                     EKNIQAILMHLSAVVDENPHVKLVIVGDGPYLSDLKELVHSLELENSVIFTGMVEHSQ
                     VAIYYKACDFFISASTSETQGLTYIESLASGRPIIAQSNPYLDDVISDKMFGTLYKKE
                     SDLADAILDAIAETPKMTQEAYEQKLYEISAENFSKSVYAFYLDFLISQKASVKEKVS
                     LTIGNKDSHSTLRFVRKAVYLPKKVFTFTGRASKKVVKAPKRRISSIRDFLD"
     gene            26494..28437
                     /gene="thrS"
                     /locus_tag="VBD27_000262"
     CDS             26494..28437
                     /gene="thrS"
                     /locus_tag="VBD27_000262"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO7024304.1"
                     /translation="MIKITFPDGAIREFESGITTFEIAQSISNSLAKKALAGKFNGQL
                     IDTTRAIEEDGSIEIVTPDHEDALGVLRHSAAHLFAQAAKRLFPDLCLGVGPAIHDGF
                     YYDTDNKSGQISNDDLPRIEEEMKKIVKENHPCIREEISKEEALELFKDDPYKVELIS
                     EHAEDGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNAMMQRVYGT
                     AWFDKKDLKAYLKRREEAKERDHRKLGKELDLFMVNPEVGQGLPFWLPNGATIRRELE
                     RYIVDKEIASGYQHVYTPPMASVEFYKTSGHWDHYREDMFPTMDMGDGEEFVLRPMNC
                     PHHIEVYKHHVHSYRELPIRIAELGMMHRYEKSGALTGLQRVREMTLNDAHIFVTPEQ
                     IKDEFLKALNLIAEIYEDFNLTDYRFRLSYRDPEDKHKYYDNDEMWENAQAMLKEAMD
                     DFGLDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPIMIHRGGISTMERFTAILIETYKGAFPTWLAPQQVSVIPISNEAHIDYAWEVARA
                     LKDRGIRAEVDDRNEKMQYKIRAAQTQKIPYQLIVGDKEMEEKAVNVRRYGSKATETK
                     SIEEFVESILADIARKSRPDEVK"
BASE COUNT         9232 a         4354 c         5809 g         9073 t
ORIGIN      
        1 ttgtgacaaa aggagagcaa ttagctaaaa cggatatcaa tatggtagac gcttgtttaa
       61 tgtaattaga aagaggtaaa gcttatgaaa agattacata aactgtttat aaccgtaatt
      121 gctacattag gtatgttggg ggtaatgacc tttggtcttc caacgcagcc gcaaaacgta
      181 acgccgatag tacatgctga tgtcaattca tctgttgata cgagccagga atttcaaaat
      241 aatttaaaaa atgctattgg taacctacca tttcaatatg ttaatggtat ttatgaatta
      301 aataataatc agacaaattt aaatgctgat gtcaatgtta aagcgtatgt tcaaaataca
      361 attgacaatc aacaaagact atcaactgct aatgcaatgc ttgatagaac cattcgtcaa
      421 tatcaaaatc gcagagatac cactcttccc gatgcaaatt ggaaaccatt aggttggcat
      481 caagtagcta ctaatgacca ttatgggcat gcagtcgaca aggggcattt aattgcctat
      541 gctttagctg gaaatttcaa aggttgggat gcttccgtgt caaatcctca aaatgttgtc
      601 acacaaacag ctcattccaa ccaatcaaat caaaaaatca atcgtggaca aaattattat
      661 gaaagcttag ttcgtaaggc ggttgaccaa aacaaacgtg ttcgttaccg tgtaactcca
      721 ttgtaccgta atgatactga tttagttcca tttgcaatgc acctagaagc taaatcacaa
      781 gatggcacat tagaatttaa tgttgctatt ccaaacacac aagcatcata cactatggat
      841 tatgcaacag gagaaataac actaaattaa taaaagagca actaattgct ctttttttgt
      901 tgtcattctt agtgattaat agtgttaaat agaaattttt ggatacatta cttagtgttc
      961 cgatatgatt tgacaccaga aataaggaca ggaattaagg taacaaatac aattcccaag
     1021 ataataagcg agaagtgctg cttcacaaaa ggaatattac caaagagaaa accagaacct
     1081 gttgcaataa ctgaccaaga taatgctgca atgaaggcgc gtttgataaa tacttttgga
     1141 gggagctgac taatacctgc tacaaaagga acaaaggttc tgataatagg gatatagcgc
     1201 cctaaaatga tggcagcagt accttttttc tcaaaataaa gctcagcatc tcggatattt
     1261 ttttctttaa tgaaacgacg aaagaaagga tgtttgataa aacggtaacc aaatgtacga
     1321 ccaatcaaga agttgcaact atcaccaata aatgctgcaa taaagaaaat aataagaaaa
     1381 gttacaatat tgaaggacat ttttggattt gcagcaaggg ctccagcagc aaataataaa
     1441 ctatcacctg gtaaaaatgg aaagatgaca gccccagttt caacgaaaat aacgaggaag
     1501 agcaatagat aggtccaatt accaacagtg ttagccatag catagatatg agtatcaata
     1561 tgtaaaataa aatcgattaa aaacatagga gtctccttta ttgatcaact aaagtgcaga
     1621 gacttggatg ataactatct tgtttataag gcctcttaca aagcctattt tatcagaaaa
     1681 tttttaataa gaggtaaaat aagtttactt attaatttag aattattcta aagagctttt
     1741 ttttgatttt tatgttagac ttaattataa tgattataaa taagaaataa aggagcttaa
     1801 caatgtccta tttaaccttt aaacacgttg attttaaaac ggatgataag ttggttttaa
     1861 atgatatcaa ctttgcaatt gatgaaggtg attttgtttc tattgtaggg ccatctggaa
     1921 gtggtaagag tactgtatta aaattagcca gtggtttaat gagtcctact gcaggtcata
     1981 ttttctttga tggaaaagat ttgaaccaat tagaaccgat tgaaagtcga aaaatgattt
     2041 cttactgttt tcaaacgcca catttatttg gtaatacagt ggaagacaat attagttttc
     2101 cttaccacat tcgacacgaa aaagtagatt atagacgcgt tgatgattta tttcaacgtt
     2161 ttgaaatgga ccagtcttac ctgaaacagg atgtcaaaaa actatcagga ggagaaaagc
     2221 aacgtattgc tctgattagg caattattgt ttgaacctaa agttttactc ttagatgaag
     2281 ttacttcggc tttagacaat cataataaag ctattgttga aaaggttatt aaatcattac
     2341 atgacaaagg aataacgatt ttgtggatta cgcatgatga ggaacagagt cgtcgatttg
     2401 ccaataaagt cttaaaagtt gtcaatggta gtattgaagc aatggaggtt ataaaatgag
     2461 cggtgcaaat tctatttctt taatgtcatt actaatggca tcgtcattag ttttgattac
     2521 tttatttttt tcttactggc aaaagttaaa cctagaaaaa gaagtgatta ttagcgctat
     2581 ccgagcagtg attcaattat tagcagtggg ttttttgcta gactatatat ttggttatca
     2641 aaatcctatt ttcacagctc ttttaatgct attcatgatt attaatgctt cttataacgc
     2701 agctaagcgt ggtaaaggga ttaataaagg gtttgttatt tcatttatag ctattgggtc
     2761 agggactata atcactttat ctgtcttgat tttttcagga attttaaaat ttgttccgaa
     2821 tcaaatgatt cctgtaggtg gtatgattat tagtaattct atggtagcaa tcggtttgtg
     2881 ctataaacaa ttattgtccg aatttagaag taaacaagag gaagtggaga caaaattagc
     2941 tttaggagca gatattttgc cagcttcgat tgatattatt cgtgatgtta tcaaaactgg
     3001 tatggtacct acaattgatt cagctaaaac acttgggatt gtatctttgc cagggatgat
     3061 gacagggtta attcttgctg gaacttcacc tatccaagcg gttaaatacc aaatgatggt
     3121 gacgtttatg ctattagcaa cgacttcgat agcttctttt gtagctactt atttggctta
     3181 taaaattttc tttaataatc gtaagcaatt agtagtaaca aaagattgat tcattttaat
     3241 aaacagtatg ttagtctggt aagtatagtc caggcttttt tggtatctta atcttatttt
     3301 tgatcgaaaa ttgaccaagt tattgagtcg attacgttta aatgctacta tttaatagaa
     3361 attgttgtag ggattatcaa aaggaggaag aaatgatttt aaaaatttgt cgtgcagcat
     3421 atagtttaca atggggaggt gtttaccaat tagctttgct ggattatcct cgaattaagg
     3481 cgtttgaatt ggaaaggata ggagctttca tagcttacga gaaacaatat aaaagaaaaa
     3541 ctgagataca atgtgacgat aaacatctcc tcgcaaaaat tgttcatttt ttaaaataca
     3601 atagttttac ttttccctat attcccaaat atagagaagc ggcagctact tttaatgagg
     3661 atggtattag tttaacttct gattttttaa gccatacatg tacgattgaa actgcaaaac
     3721 taatttttaa agaaggtaaa atcttatcag cagttaaagc ctttaataag cctgctgaag
     3781 tactggtaaa agataagagg aatgctgctg gagaccctaa agattacttt gactatgtga
     3841 tgttgaactg gtcaaatacc aattctggtt atcgtttagt aatggaaaga ttgttaggca
     3901 aagcaccatc tgaacaggag ttaacagtag gttttaagcc aggggtcagt tttcatttta
     3961 cttatcaaga tatcatcaat catcctgatt ctatttttga tggttatcat cctgctaaaa
     4021 ttaaaaatca gctttcttta gcagaacatt tagttgcatg tgttatccca aaacattatc
     4081 aagaagatta tcaaagcctt gtgcccaatg acttgaaaca cagggtttat tatttagatt
     4141 actgtaacga aacactttat gagtggaatc aaaaagttta tgattttctt tgtcatttgg
     4201 aaaataaata aaaccaaaaa gtttaaaact ttttggttta actgctatta attggtcttt
     4261 aaaagactta aaaattcttc aatcatagga ctaaaagaag tatcatttcg gtaagcaata
     4321 ccaatcggat aaaaattatt tttatcctta agtgggaccc agcataaatc ttgaacttgg
     4381 ctaatgcttt caaattcact tggtaaaata gtgactgcat tgaggtcttg taaactatga
     4441 ataagaactt cccagtcatt gtgtttaaag acgatatggg ggtcaaatcc taaagaacga
     4501 catttacgag gcaacatctc tcctagcata taatgttcgt tgagtgaggc aactttataa
     4561 tctcttaaat cattaagttc aatttcagga agtgttgcaa gaggatgcga ttttggcatg
     4621 acaatgctta ctttatatcc tttagttgag gtttgcagtg gttcgatagt gatatcattg
     4681 cgggtacttg gaaaggacaa taagccaata tcgatctttc cttttgctaa caattcttgt
     4741 aatttacggg aaccatcttg aatcaaagat acttcaacat ttgaatgtgt agccataaaa
     4801 gttgaaatct gtttcataaa ttgaatagca aaaagagaag taagccctac acggatacct
     4861 tccttttttt cttgatttaa ttgctggatc tccgtaacca tttgattaac attagtcatc
     4921 aacaattgtc ctttctcata gagaatttct cctgcttcag ttaaagcgag ttgacgacca
     4981 ttttgtaaga ataaaatagt atttaattct gcttctaatt ttttaatgga ttgtgaaagt
     5041 gtaggttggg tcacaaaaag actttttgca gcgcgtgaat aattttttgc ttctgcaaca
     5101 gcaataaagt aagtaagttg tctaatatcc ataatatctg tccttttttc ttagtaatag
     5161 tatagcacaa gtctatcaat ctgaccttta tgattatttc cacaataata ttagtaaaag
     5221 ctatacttat ataatttttt ccaattttcc ttttgcaaat agctatgata tagttaatat
     5281 agttagtgaa atcacaaaca atcattatta gtaggaggca tcttatgtcg gatgttgtcg
     5341 aaaaacaaac aactaaatcg tttataatga acgtattaaa tggtttagct ttagggactg
     5401 ttattgtctt gataccagga gcaatccttg gagaattaat gaaagcttta ctgccaatgt
     5461 ggagcggctt tgcaacttta atcgctgcaa cagcagttgc aacaagtatg atggggcttg
     5521 ttattggtat tatggttggt ctcaatttta aatttaatcc cattcaatca gcatcactag
     5581 gattagcggt tatgtttgca ggtggagctg caaccttcct taaaggcgct attatgctaa
     5641 aaggaacagg cgatatcatt aacatgggaa ttactgctgc cttaggtgtc cttttgatcc
     5701 aatttttatc tgacaagacg aaatcattca ctttgattgt cattccaaca gtaacactct
     5761 tactagttgg aggagtaggt catgtgttat taccttatgt caaaatgatt accaccatga
     5821 ttggacaagg gattgcttca ttacttggtt tacaacctgt tttgatgtca attttaattg
     5881 cgatgatttt ctgtttcttg atcgtatctc caatcacaac agttgggatt gctttggcaa
     5941 ttagtttgtc cggaattggt tctggtgctg ctaatttagg tatctgtgct gctagttttg
     6001 ggctttgtat ggctgggtgg tcagtcaatt ctaaggggac agccttagcg catgttttag
     6061 ggtctcctaa gatttcgatg gctaatgtat tagcaaaacc gaagattatg ctacctatga
     6121 tatcttcagc agcaatacta ggaattctcg gagctctctt taatattcaa ggaacaccag
     6181 ctagtgcagg ttttggtatc agtggtttga ttggaccgat taatgctctt aatttagcaa
     6241 aaggtggatg gtcagttatg aatatgttat tgataattat aatatttgtt gctgcaccaa
     6301 ttatattgaa ttttattttt aattacctct ttataaaagt acttaaaatt attgatccaa
     6361 tggactataa attagatatt taaggagata ttagtgatga aactcaaagt atttaatgtt
     6421 agagaagaag aagcaacatt agcacaagat tgggcaaacc gaaatcatgt ggaactttct
     6481 atgagtgaag gacctttgac attggaaact gttaatgagg tagaaggttt tgatgggatt
     6541 gctaatgcac agattgagcc tttagatgat gctatttatc ctcttctcaa agaaatggga
     6601 attaagcaaa tcgctcagcg cagtgctggt gttgatatgt ataatcttga gttagctaag
     6661 caacatggta ttatcattag caatgtgcca agttactcac cggagtctat tgcagaattt
     6721 acagtgacaa tcgctcttaa ccttattcga aaagttgagc ttattagagc taatgttcgt
     6781 gaacagaatt tttcatggac cttgccaatt cgaggacgag tcctaggtaa catgactgtg
     6841 gctataatag gaactggtcg cattggtcta gcgactgcta aaatttttaa agggttcgga
     6901 tgtcgtgtta ttggttatga tatttaccat aatccaatgg cagatggtat ccttgagtat
     6961 gtcaattcgg tagaagaagc tgttgaagaa gctgatcttg tgtccttaca tatgccacca
     7021 actgctgaaa atacgcatct atttaattta gatatgttta aacagtttaa aaaaggtgct
     7081 attttaatga atatggcacg tggagcattg gtggaaacca aagatcttct tgaggcttta
     7141 gatcaagggc ttttggaggg agctggtatt gatacttatg aatttgaggg gccatatata
     7201 cctaaaaatt gtcaaggtca agacatttca gacaaggatt tcctaagact gattaaccat
     7261 ccgaaagtaa tatacactcc tcatgcggct tattatacag atgaagcggt caaaaatcta
     7321 gtagagggtg ccttaaatgc ttgtgtagaa gtgattgaaa cgggaacaac tactacgaaa
     7381 gtcaactaaa agatgcttag gcatcttttt ttgtggggta acaaaacata tgatgattat
     7441 gacctttaaa gcaaaaaaac atcttatgta tttatagctg gaaagtcttt tttataataa
     7501 aaaagatatt ttttataaaa atattgaaaa aaacatcaga tgaatagtat tatttaatca
     7561 taaaagcgtt tacaaaatac aaggagggtt ctctcatgga gaaaacaatg gatatggaac
     7621 aacgtgaaga agttgtatct tataaccgag cgaaagtatg gcagttagtt atgtttgcca
     7681 tgaacaatgc ttctacaaat atatacttat ttacgtttat gtttgttacc tatttctcaa
     7741 ctggcgttct tggctcggca gccatttttg tcagtcaaat tatgggttat atccgtattt
     7801 ttgatggttt tattgatcca gctattggta ttatgattga taaaactgat acaaaatttg
     7861 gtaagtatcg tcctattttg attattggga acgttattac ggctttgtca ttgatttttc
     7921 ttcttgcatt gagaggtgtc gatgaaaata ttcgtttccc attatttatc cttgttctaa
     7981 ttatccataa aattggatac tcaatgcaac agacaataac taaagcaggg caaacagcac
     8041 tgacaaatga ccctaagcaa cgtcctattt ttaatatcgt tgatgcagtt atgacaacat
     8101 cattaatgac aggcggtcag tttgtcgttt cagttttctt agtaccaaaa tttggtaatt
     8161 ttacacctca attcttcaat gtccttattt ttggaacaat tcttatttca gctattttag
     8221 caatcgttgc aattattggg atttgggcaa aagacagaaa agaattcttt ggtttaggag
     8281 agaataccca aaagactgct ctaaaagatt actggaaggt gttgaaagga aataaaccgc
     8341 ttcagatatt atccattgct gcagctttgg taaaatttgc catccaattc tttggcgatt
     8401 ccgttgttat ggtgctattg tttggtattt tatttggtaa ttatgcttta tcaggacaat
     8461 tctcacttct tttcattgtt cctggtgtta ttattaatat tttgttctca acaattgctc
     8521 gtaaaaaagg tcttcgtttt tcatatgtca gagcaattca gatcggtatg attggtttac
     8581 ttgcttttgg tgcagttctt tacgttggta agcctggtga ccttagttta acaagcctaa
     8641 atctttatac tattttattt atcgttacta atattattgc gagatacgct tcacaagcac
     8701 ctgctagttt agtattgact atgggagcag atatttctga ctatgaaact tctgaatccg
     8761 gtcgttatgt atcaggaatg attggaacta tcttctcctt aacagattcg attgcttctt
     8821 catttgctcc tatggtagta ggttttgtgt tagcaggaat tggattttca aaatcatttc
     8881 caactatcga aacaccactg ccccctgatt taaaaatggc agctattagt atccttgtgg
     8941 ccattccatt tatagctttg tctatagctc ttcttttaat gaaattttac aaactagata
     9001 aagaagaaat ggttcgtatt caagagaaga ttcaagttat gaaagcagca actactaagg
     9061 aacgtgctaa agatattgct cgtaatgttc ctttgacaga tatggattat gttgatgtaa
     9121 caaaatatcc tattgataaa gattaaaaag tttagctgct cttcaataga gcagctattt
     9181 tgttaaataa gagctaccat tggaggattc ttatggcaaa aattatttca ttaggtgagg
     9241 ttcttttgcg attatctcca cctcaatatc acacactgat gcaggctaat cacctaaaat
     9301 gccaattcgg tggttcagaa cttaatgtct tagcaagcct tgctcaacta ggttaccatg
     9361 taggattggt ctcagctttg cctgataatg atttgggcaa gatggcaagt caatttatat
     9421 tgtcacagca aattagtcca gctgctataa ttaaaaaaga aggacgacta ggaatttatt
     9481 actatgaaca aggattttcg gtaagaacaa ataaggttat ctacgatcgt aactattcat
     9541 ctttttggga aagtaccttg agtgattatg attttacttc tatttttaag ggagtggatt
     9601 ggtttcatgt ttctggaatt actccagcac taaccaagga cttatatgag gtgactcggt
     9661 tccttatgac aaaagctaaa gaaggaggtg taaaagtatc gattgatctc aattttcgtg
     9721 agagtttatg gtcatctttt caagaggcac gtgagcaatt atcgccacta ttaggattat
     9781 tagatgtttg ctttggctta gagccgattt atttagctgg cgaaagtgaa gatttaaaag
     9841 atgagttggg cttatccaga ccttaccttg atattgaatt acttgaaaag ataacccaaa
     9901 agattgtcca agaatatggg ttagactata ttgcctttac tcaaagggaa atggaatata
     9961 ctaatcagta tatgttaaaa tcatatttgt atcataataa tatgttatat caaactgata
    10021 aaactggcgt tgaagtatta gatcgtgtgg ggacaggaga tgcctttgct gcaggtttaa
    10081 ttcatgcgct tctggaaaaa gaaactcctc aaagggcctt agaaatagct atggctacat
    10141 ttaaatacaa acataccatt caaggagata tcaatatcat gacacgagat gatattgctt
    10201 acctcattga aaaagaaaca aatgatatta aacgatagtg taaaaggaga gtgtatgtta
    10261 tatccattat tgacaaaaac aagaaatact tatgatttag gcggtatttg gaactttaaa
    10321 ttaggagaac ataatccaaa tgaattacta ccttcagatg aagtcatggt tatcccgact
    10381 tcatttaatg atttgatggt aagtaaagaa aaacgtgatt atataggtga tttttggtat
    10441 gaaaaagtca ttgaagtacc taaagtatca gaggatgaag agatggtgct gcgttttggc
    10501 tcagtgacac atcaagctaa aatttatgtt gacggtgtat tggtaggaga gcataaggga
    10561 ggttttactc cttttgaagt tcttgttcct gaatgtaagt ataataatga gaaaattaag
    10621 gtatcaattt gtgctaataa cgttttagac tataccacgc ttccggtcgg aaattatagt
    10681 gaaatcattc aagaagatgg tagcattaag aagaaagtac gtgagaattt tgatttcttc
    10741 aattatgcag gtgtgcatcg tcctcttaag ttgatgatac gtcctaaaaa tcatattttt
    10801 gatattacaa ttacaagtcg tctatcagat gatttacagt cagcagattt acatttttta
    10861 gtggagacta atcaaaaggt tgacgaagtt agaattagcg tttttgatga agataacaaa
    10921 ctagtgggtg aaacgaagga tagcagatta tttcttagtg atgtccatct ttgggaagtt
    10981 ttaaatgcct atctgtacac agcgcgtgtt gaaatttttg ttgataatca actccaagat
    11041 gtctatgagg aaaattttgg tcttagagaa atagaagtga caaatggtca attcctattg
    11101 aatcgcaaac ctatttattt taaaggattt ggtaaacatg aagatacgtt cattaatggc
    11161 agaggtttga atgaagctgc taacttaatg gatttgaatc ttttgaagga tatgggggca
    11221 aactctttta gaacatccca ttatccttat tctgaagaaa tgatgcgttt agcagatcga
    11281 atgggagtgt tagtgataga tgaggttcca gcagtagggt tatttcaaaa ttttaacgct
    11341 tccttagatt tgtcaccaaa agataatggt acgtggaatt tgatgcaaac aaaagcggcg
    11401 catgaacaag ctatccaaga attagtgaag cgtgataaaa atcatcctag cgtcgtgatg
    11461 tgggtagtcg ctaacgaacc ggctagtcat gaagcgggag cacatgatta ttttgagcca
    11521 ttagtaaaac tttataaaga tttagaccct caaaaacgtc ctgtcacctt ggttaatatt
    11581 ttaatggcaa ccccagatag agaccaagtg atggacctgg ttgatgttgt ctgccttaat
    11641 cgttactatg gttggtacgt tgaccacggt gatttaacaa atgcagaagt aggtataaga
    11701 aaagagttat tagaatggca agataaattt cctgacaaac caattatcat aacggagtat
    11761 ggcgctgata cgttaccggg attacattct acttggaata ttccatatac agaagaattt
    11821 caatgtgatt tttatgaaat gagtcatcgt gtttttgatg gtattcctaa tttagttggt
    11881 gagcaagtct ggaattttgc agactttgaa actaatctga tgatacttcg tgtacagggg
    11941 aatcacaaag gtctcttttc aaggaatcgc cagccgaaac aagtcgtcaa agaatttaaa
    12001 aaacgctgga tgactattcc tcattaccat aataaaaaaa atagtgtaaa ataggggtag
    12061 ttattttaga agaggtgatg atatggctag acccttagta gaacaaacag cagatcgtct
    12121 attgcattta attttagaaa gagaataccc tgttggtgct aagctcccca atgaatatga
    12181 attagcagaa gatctagatg ttggtcgcag cacaatccga gaagcagtca gaagtttagc
    12241 aacgcgtaat attttagaag tcagacaggg ctctggaaca tatattagtt ctaaaaaggg
    12301 tgtttctgag gatccattag ggttctctct aataaaggac acagataggt tgacatcaga
    12361 tctctttgaa ctacgccttt tattggaacc tcgaatagca gagctggtag cttatagaat
    12421 tacagatgat cagttacaat tactcgaaaa gcttgttgga gatattgaag atgctgttca
    12481 cgcaggcgat ccgaaacatt tacttttaga cgtggaattt cattccatgc tagctaaata
    12541 cagtggtaat attgcaatgg atagcctttt gccagtcatc aaccagtcaa ttcatctgat
    12601 aaatgcgaat tatacaaatc gtcagatgaa gtcggatagt ttagaagcac atagagaaat
    12661 tatcaaagca atccgagaaa aaaatccagt agctgcacat gatgctatgc ttatgcatat
    12721 catgagtgtt agacgttcag cgttgaaagc ttaatataag aagaaaaata gctattcttg
    12781 aatagctatt tttttagtat tcttcttgac aattcatcag atgacttata taatatagtt
    12841 aaagataacg gttacatttt gggaggtatt atgttaaatc aattgaaaaa caactatttc
    12901 tttgctgtta ttcgagggaa atcatctgag gatgcgctcg aaatcgcaaa gcatgctatt
    12961 ttgggtggta ttcggaatat tgaagtaact ttttcgactc ctgaagcaag caaagtcatc
    13021 aaacaattat cagatgactt taaaaataat aaagaaatta ttgtgggggc cggaacggtc
    13081 atgacaactg aacttgctaa agaggctatt gatgctggag ctaaattttt ggttagccca
    13141 cactttgatt cagatattgc taatttagct aatgaaaaca aggtttatta tttcccaggc
    13201 tgtgcaacgg caacagaaat cgtggtagct agaaagtata aatgtcagat tataaagctt
    13261 tttccaggtg gagttgtggg ttcaggtttt atcaaagata ttcatggtcc tattccagat
    13321 gttgatttaa tgccatcagg tggcgtctct gtttcaaatg tagttgaatg gagaaaagca
    13381 ggagcagttg cggttggtgt tggctcagca ttatctagta aagtagccac agaaggctat
    13441 gatagtgtta ctaaaatagc taaacagttt gtttctgcac ttgattagtg agtaagagaa
    13501 gaatatggcg tttaatactg aaacatttat gttaaaaaat caagcagcta tacagctgta
    13561 tgaagaggtc aagagacaac ctatttttga ttatcattgc cacttagatc caaaggacat
    13621 ttttgaagat catatttttg ataatattgt ggatttgtgg cttggtggtg accactataa
    13681 atggcgtctt atgagggcta atggtatttc tgaagcagaa attacaggtc cagcatccaa
    13741 ccttgagaaa tttaaagcat ttgctagaac actagaaaga gcatacggta atcctgttta
    13801 tcattggtct gctatggaat tgaaaaatat ctttggtgtt aatgagattt tgacagaatc
    13861 aaatgctgag gaaatttatc atcgtttaaa tcatttcttg aaagagcata aaataagtcc
    13921 tagacgttta attgcagata gcaaagttat gtttattggt actacagacc accctcttga
    13981 tactctcgag tggcataaaa agttggctgc tgatgagagt tttaaaacag ttgttgctcc
    14041 gacatttagg ccagatgaag cttttattga acatcgacat tttgttgatt ttattactaa
    14101 actgggtgac attactcaaa aagagattac agatttttca acatttatag cagcaatgga
    14161 agaacgcatt gcgtattttg ctcaaaatgg ttgtagggct agcgatatca gttttacgga
    14221 aatcgtcttt gaacaaactg atgaattgga gttgaacgat ttatttaata aagtttgtga
    14281 aggctatatc ccaaatcaat cagaaatcag caaatggcaa acagctgttt ttatggaact
    14341 ttgccgatta tataaaaaat atggttttgt aacacaggtg cattttggag ctttgagaaa
    14401 taatcattct actatttttg aaaaattggg agctgacgtg ggtgtggatt cacttggtga
    14461 ccaagtggct ttaactgtta atatgaaccg tttattggat tccttggtta aaaaagatag
    14521 tctgcctaaa atgatttggt ataatctcaa tccagcttac aatatagcag tagctaacac
    14581 tttggcaaat tttcaagcta acgaacttgg agtgagatct taccttcaat ttggtgcagg
    14641 ttggtggttt gcagatacta aattagggat gattagtcaa atgaatgcct tagcagagca
    14701 aggcatgttg gcgaatttta ttggtatgtt aacagattct agaagttttc tatcttatca
    14761 acgtcatgac tattttcgac gtattttatg tacctactta ggagaatgga ttgaagaagg
    14821 agaagttcca gaagattatc aagctttagg aagtatggct aaagacatcg cttatcaaaa
    14881 tgcagttaat tattttaaga actaaaaagg aagagtgaaa tcatggaaat gtcatttagg
    14941 tggtatggtg aagacgaccc cgttacttta gaaaatattg gtcaaattcc aacaatgaaa
    15001 gggattgtta cagcgatata cgatgtacca gtaggagaag tttggtcacg tgagcgcatt
    15061 caacagctga aagaaaaggt agaggcagca gggcttaaaa tttcggttat cgaatcagta
    15121 cctgttcatg aagatatcaa attgggacgt ccaacacgtg atctcttgat tgataattat
    15181 attcaaaccg tcaaaaattt ggctgcggaa ggtattgata ctatttgtta taattttatg
    15241 ccagtatttg actggacacg cactgattta gcatatcaat atccagatgg ttccacagct
    15301 ttgatatttg atgagacggt ttctaaaaaa atggatccag taaacggtga attatctttg
    15361 ccaggatggg atgccagcta tagtaaagaa gaaatgaagg ctatcatgga tgcctatgct
    15421 gaaattgatg aggaaaaact ttgggaaaat ctcacttatt ttattaaacg tattattcct
    15481 gaagcggaag ccgtaggcgt taaaatggcc attcatccgg atgacccacc atattctatt
    15541 tttggtttac ctcgcattat tactgggtta gaggctattg agcgttttgt gaaattgtac
    15601 gatagtaaaa gtaatggcat cactttatgt gtgggttctt acgcttctga tccccagaat
    15661 gatgttttag agatttcgcg acgtgctttt gagttagatc gtgtaaactt tgttcatgct
    15721 cgcaatatta aattaggtga tggaaagtca tttaaagaat cggctcatcc atcagaatac
    15781 gggtcaatag acatgtatga agttattaag ttatgccatg aatttgggtt tgagggtgct
    15841 attcgacctg accacgggcg tatgatttgg ggagaaacag gtcgtccagg atatggccta
    15901 tacgatagag ctttaggagc cacttacgtt tctggtttat acgaagctgt gattaaaggt
    15961 tcaaaataag gcgatgcttc caaatttctc ctgttccaat ctcctgaaag agaaatttgg
    16021 aaagttatta tcataatatg attgtactta gctaagagta gaatacttat agaagttaat
    16081 gagaggaata agatgtcaaa aacaattaca tttaccaata aagttgtcgt cataacaggt
    16141 gcaggcggtg ttctctgtgg ttatatggct aaagaatttg cgaaagcagg cgctaaagtg
    16201 gcattattag atttgaatca agaagctgct caaacatttg ctgatgaaat tgtagaagaa
    16261 ggtggtatcg ctaaggctta taaagctaac gttttgtcta aagaaaacct tgaagaagtg
    16321 catcaagccg ttttagagga tcttggacca actgatattt tagttaatgg tgcaggaggc
    16381 aacaatccta aagctacaac tgataatgaa tttcacgaat tagacttacc ttcagaaaca
    16441 aaaaccttct ttgagttaga tgaagctggt attagctttg tttttaactt aaattattta
    16501 ggaacattat tgccaacgca agtatttgcc caagatatgg ttggacgtga aggagcgaat
    16561 attattaata tctcttcaat gaatgctttc acccctctga ccaaaatacc agcttattct
    16621 ggagctaaag cagctattag taacttcaca caatggctag ctgttcattt ctcaaaagtt
    16681 ggtatccgtt gtaatgctat tgcacctggt ttcttggtaa caaatcagaa ccgaagcttg
    16741 ttatttactg aggacggtca gccaactgcg cgtgctgaga aaattcttaa taatacacca
    16801 atgggaagat ttggagaagc aagtgagctg attggcggat tgttcttttt agcagatgaa
    16861 aaatcatcaa gttttgttaa tggcgttgtc cttccgattg atggtggatt tgctgcttac
    16921 tctggtgttt aatttacaag ataatagcca cttaacacat aagagattat tgagttgagt
    16981 ggttttttaa ggaaatttgt ttaatgatct taccactaaa tgatgggtag gttgctaatt
    17041 ggaaaatcct cataagttag ataaatttag aaactggaga taatatggta gatacaaatc
    17101 acaaaatcgc tttttgcata gattctgatg gttgcgcaat ggatacaatg acatataagc
    17161 atcaactctt ttttggtcca attgctgcag atatatttgg gatatcagaa caagaaagaa
    17221 caagtttttt aaaagagtgg gatagagtca atctttattc aagaactcgt ggagttaatc
    17281 ggtttgtggg attggttatg ggccttgagt ttgccggagt agaacatatt gataaattaa
    17341 aagaatgggt cgaaacaaca gactcattgt ccaataattc cttagagtta gcgcttgcca
    17401 acgattttag tagagattta gagaaggctt tagaatggtc caatgaagtg aatcgccaaa
    17461 tcaaaaatta tgagggagat tctttagcgt tttcaggtgt gttagaaagt ttagaacgat
    17521 taagtcaatt tggtaatgtt tatgtcgtga gttctgctaa caaggaagct gtttggcaag
    17581 agtgggaaga acagggattg ctaaagtatg ttgatgatat ctattgtcaa gatagaggta
    17641 aaaaagaaga tgttatcgcc actttaattg caagtggtta ctgccgtgaa actattttga
    17701 tggtaggaga ttccctgggt gatctggctg cagcagaaca aaatggtatt gcattttacc
    17761 cagtacttgt tggtaaggaa gtcaaatcat gggaaattct tagagaagat attggtgaag
    17821 cttttgcaaa aggtcaattc gaacagcaaa ggcaaaaaga atctattaat accttttggg
    17881 ctaatttaga taactaatag aaaggatagg ctatgacaca tttagttgac ttaacaaaga
    17941 aacccttcaa tctaaatcaa gaagcaattg agtggattga aaaaacaatt aatgagatga
    18001 cattggatga gaaaattggg caactttttt tcaatatggg agctagtcgt tcagaagaat
    18061 atttaacaga tgttttggat cggtatcata ttgctgctgt tcgctataat agaggtagtt
    18121 caagtgaaat ctatgatcag aatttaattt tacagacaaa gtcgaaattg ccgatgttga
    18181 ttgctgcaaa tacagaagct ggtggagacg gtgctgtcac ggatggaact aaagttggcg
    18241 atgaaataaa agtggctgct acaaatgatc cgaaatatgc ttatgaaatg ggaagaatcg
    18301 cagggatgga ggcatctgct gtgggttgta atgcttcatt ttctccaatt gtagatttga
    18361 cacgtaattg gcgtaatcct atcatagcta gtcgaaattg gggtgctaac gttgatcaaa
    18421 ttatttcact ttcaaaagag tatatgaaag gaatcatgca atataatatt gtacctttcg
    18481 ctaaacattt tccaggggat ggcattgatg agcgtgatca tcatttgtca tttgcttcca
    18541 atcctatgag taaagaggag tggatgtcga catttggccg catttatggt gaattggctg
    18601 acgcaggttt accaggagtc atggcaggtc acatacattt accaaacgtt gaaaaagaaa
    18661 tgcatccaga gcgagacctt gatgatatgt taccagcttc attgaataaa acacttttag
    18721 atgagttatt gagaggagag ttaggttata atggagctat tgtaacggac gcttctcata
    18781 tggttggaat gactgcttca atgccaagga gagatttgtt gccaacggcg attgaagcag
    18841 gttgtgattt attcttattc tttaatgacc ctgatgaaga cattcaatgg atgaaagaag
    18901 gttacgaaaa aggtattcta acagaggaac gtctccatga cgctttacgt cgtactctcg
    18961 gtctaaaagc gaaattaggc ttgcataact atgagggacg tcgccaagaa ctctttatgc
    19021 ctaaagataa ggctatggct ttaatcaaca cattagagtc gcaaaaaata gctgatgaag
    19081 ttgctgataa agcagtaact ctggtaaaag ataaacaaaa agatatcttt ccagtaaatc
    19141 cagaacgtta tcgtcatatc ttacttgtta acgttgaagg atataaggga ggttttggtg
    19201 cgatgattgc tggaaacaag caacgtgcat cggattacat gaaagagtta cttgaggcac
    19261 gtggccatga agtaactgtc tgggagtcta ccgaagagcg tattatgaaa ttgccacaag
    19321 aggagcgggc agcagctatc gctaatgttt acgcacaaaa acagccaatc gcaaatctaa
    19381 cagaacacta tgacttgatt attaacttgg tagatgtcaa tgctggagga acaacacaac
    19441 gtattatttg gccagcagct aaaggcaccc ctgatcagcc tttttacgtt catgaaatac
    19501 catctattgt gatttcagta caacatgcat ttgctttagc agatatgcca caggtaggga
    19561 cttatattaa tgcttatgat gggcttccta gcacgataag tgcagtagtt gcgaaattgg
    19621 ctggagaatc agaatttaca ggagtttcac cagtagatgc ttactgtggt ttaattgata
    19681 cacatttatg gcgccaatgc taatagaaac aagatgatgt acttttatgt aacataaaaa
    19741 aagagcttaa agctcttttt ttatccttca aaatagagta gttccttggg tgttttggta
    19801 aatacttcaa agccggtttt agtcacataa ccgcagtctt caattcgcac accaacttta
    19861 tctggtatat aaatacctgg ttcaacagag aagcacatgc cttcttgaat ctccatatcg
    19921 ttaccagcca taatagatgg aaattcatgg acatccatac ctaatccatg accaaggcgg
    19981 tggttaaagt attgtccata accagccttt tcaataacat ttcttgctgc tgcgtcaact
    20041 tcagaagcaa ggactcctgg cttaataaaa tcaatagccg cttgatgtgc ttcaagacat
    20101 aaatggtaaa tatctttttt gaattgatca ggtttcccca ctgctactgt acgggtcata
    20161 tctgacgtat aacctagtgt ttctacacct aaatcaaata ataacaaagc attattttcg
    20221 attttattag ttcctgggat accatgtgga tttgctgcat tattgccagt caaaaccata
    20281 gtgtcgaaac tcattttatt gataccttgt tttttcattt caaattcgat ttgagcgata
    20341 atatcggttt cagtgttgtt tagcgagata ttgtcaaatc caacttgaac agctttatca
    20401 gcaaattctc cagcgactaa cattttctca atttcatctc gtgatttaat taggcgcatg
    20461 ttttgaatat aaggtgttag gttttcaaaa tgaccatcaa aaacggtttg gagaccttgg
    20521 aatttagtaa cattaagatt atcaaattcg gctagaactt tagaaacaga aaaggatggt
    20581 aagttgctag ctattttttg ccaaggattt tctgaatcta tgtaaccaaa gacgggaaat
    20641 ggtacacttt gttttgcacg cgatacttct agtgctggta caaataaaat aggatcgcga
    20701 tcttcataga caaataaaaa catttgtctt tcatggggat cgcaaaagaa acctgttaaa
    20761 tagttgactg tcacaggatc agaaaagaca gcaagttcag cttggactga atgtaagtga
    20821 tggcgtatac gattaagttt tgacataata acccttcttc ctaaaaggaa cttagtctag
    20881 ttaggctagt ttcttatgcc tattattttt ccaaaaaaat aggaaaaaat caagtttagt
    20941 tagtattttg aaagtttttc ataaaaaagc tttataaaag cttgaaagtg tttccaaaag
    21001 gtgataaaat atcagtgaaa gcgtcatttt caaatagaaa ggaagttaaa tcaatatgaa
    21061 tacagatgat acgattacga tttatgatgt tgcccgtgaa gcaggtgttt cgatggcaac
    21121 tgttagtcgt gtggtaaatg gaaataaaaa tgtcaaggaa aatacacgta agaaagtcct
    21181 tgaagttatt gatcgtcttg actatcgtcc aaatgctgtg gcgcgtggtt tagcaagtaa
    21241 aaaaacgaca actgttggtg ttgtcattcc taatatcgca aatagctact tttcaatttt
    21301 agcgcgtggt attgatgata ttgcagcaat gtacaaatat aacattgtcc ttgcttcaag
    21361 tgatgaagac gatgataaag aggttaatgt tgttaatact ttgtttgcta agcaagtaga
    21421 cggtattatt tttatgggac accacttaac tgaaaagatt cgtgcagagt tctctcgttc
    21481 acgcacaccg attgttttag caggtactgt ggaccttgag catcaattac caagtgttaa
    21541 tatcgattac aaagcagcag cagttgatgt tattgatatt ttagctggca atcataaaga
    21601 cattgctttt gtatcaggac cacttattga cgatattaat ggcaaggttc gtttggcagg
    21661 atacaaagaa ggtctcaaga aaaatggctt aaactttaaa gaggggttgg tttttgaagc
    21721 caactaccgt tatgctgaag gctttgcatt agctcaacgt gtgattaatg ctggagcaac
    21781 agcagcatat gttgctgagg atgagttagc ggcaggtttg ttaaatggtc tttttgaggc
    21841 aggtaaacgt gttcctgaag attttgaaat tatcacaagc aatgactcgc caatcgcaca
    21901 atatacacgt cctaacttaa cttctatcag tcaaccggtc tatgatttgg gtgctgttag
    21961 catgcgtatg ctaacaaaaa ttatgcacaa agaagagttg gaagaaaaag aaatagtgct
    22021 taatcacggg attgttaaac gtggcacaac aaataattag tgttagagta tgttatttga
    22081 aaaatagtgg tagaatggta gtagcttagc gcttaacttt ctgatactta acatgatata
    22141 ttgagaggat tagggtagct tagcttccta atcttttttt agaggtgtaa tatgacaaat
    22201 gaattgataa tgcaagcttt tgagtggtat ttacctagtg atgggaatca ctggaagaaa
    22261 ttagaggagt ctatatcaga ccttaaaaaa cttggaatta gtaaaatctg gttaccacca
    22321 gcatttaagg gaactagcag tgatgatgta ggatatggtg tttatgatct ctttgattta
    22381 ggagaatttg accagaatgg aacaattaga acaaaatatg gtaggaaaga agagtatcta
    22441 aagcttatta agtcgttaaa ggcaaatggc attaaaccgt ttgcagatat cgttcttaac
    22501 cataaagcca atggtgatca taaagaaaaa tttcaagtca tcaaagtcaa tcctgaaaat
    22561 cgtcaagaag cattaagtga accctatgag attgaaggat ggacgggatt tgatttccca
    22621 ggtagacagg gtgagtacaa tgattttaaa tggcactggt accactttac tggtttagat
    22681 tacgatgcta aaaataatga aacagacatc tttatgattg taggcgataa taaaggttgg
    22741 gcagacgatg atttgattga tgatgaaaat ggtaatttcg attatctcat gtacaatgac
    22801 attgatttta agcatcctga agttataaaa aacttacaag attgggctaa gtggtttatt
    22861 gaaactacag gcattgaagg ttttcgtttg gatgctgtga aacatatcga ttcttacttt
    22921 attcaaactt tcattaatga tattcgtaca aaaataaaac ccgatttaga ggtctttggc
    22981 gaatattgga aatctgacca aacatcaatg aaggactatt tagaggcaac acaatttcaa
    23041 ttttctcttg ttgatgtcac cttgcatatg aatttttttg atgccagtca ccaaaaccgt
    23101 gattttgata tgcgaacaat ttttgatgat agtcttgtta ttgataatcc tgagtatgct
    23161 gttacattcg tagaaaatca cgatacgcaa agtggacaag ctctagaaag tagagtggaa
    23221 gattggttta aacctttagc ttatggcttg atacttttac gccaacaagg gactccatgt
    23281 cttttttatg gtgattacta tggcattcaa ggagaatttg gccaaccatc ctttaaagag
    23341 gttattgata aaatggctga gctacgccaa aactatgtat ttggcgaaca agtcgattac
    23401 tttacgcatt caaattgtat tggttggact tgtcttgggg atgaggaaca taattcttgt
    23461 ttagctgtgg tcctaacaaa tggtgatcag ggatggaaac atatggaagt aggggaaatt
    23521 tatgcaggca aaacttttgt tgattattta ggcaattgtg aacaagaggt tgttatagga
    23581 gatgatggtt ggggtgattt tttagttgca tctgcgtcta tctcggcttg ggtccctaaa
    23641 atagaggaag ctaactgata aatcaggtga aaaaaaaggt aaaatgagat ataataagac
    23701 ctatgaaagt tttactgtat ttagaggctg aagaatactt gaaaaagtca ggtatcggtc
    23761 gtgctatcaa acatcaagag aaagctcttc aaattgctgg gatagattat accactaatc
    23821 ctacagatga ctttgacctt gttcatatga atacctatgg tattagaagt tggcttttga
    23881 tgagtaaagc taaaaaaact gggaaaaaag taattatgca tggccattcc actgaagagg
    23941 actttagaaa ttcttttatt ggctcaaatt tagtatcccc gctttttaaa tggtaccttt
    24001 gccgttttta tcaaaaagca gatgccatta taaccccaac tgattattct aaacaactaa
    24061 taaaagcata tggcatcaag aaaccaattt ttgttttatc taacgggatt gatctaagtc
    24121 gctatcagag gtcagaaaag aaagaagctg catttcgtca ctattttcac ttaagtaagg
    24181 atgataaagt tgttatgggg gccggtcttt atttcatgcg taagggaatt gaccaatttg
    24241 ttgaagtagc tgcaaaaatg cctgatattc gttttatttg gtttggagaa acaaataaat
    24301 gggttattcc tcgaaaggtt aggcaaatcg ttaccaagca acatccctca aatgttactt
    24361 ttgctggtta cataaaaggt gatgtttatg aaggtgccat gagtgctagc gatgctttct
    24421 tctttccgag tcgtgaagag acagaaggga tagtagtttt agaggcttta gctagtcatc
    24481 agcatgtggt tttaagagat atccctgtgt atcacggttg ggtgaccgaa gacagtgttg
    24541 aattagcgac tgatgttgat ggttttgttg aaaagttgga taaagtctta tctggtaaga
    24601 gtgataagat taaagaaggt tatcgtgtgg ctgagagtcg aagtatcgaa cgaattgccc
    24661 atgaattggc aagtgtttat caaaaagtaa tggagttgta agatgcgtat aggtctattt
    24721 acagatacat actttcccca agtttcggga gtttcaacaa gtatccgaac tttaaaggaa
    24781 gggttggaga aggaaggaca cgaagtttat attttcacaa ctaccgaccg taatgtcaaa
    24841 cgttttgaag accctacaat tattcgttta ccaagtgtcc cttttatttc ttttacagat
    24901 cgtcgagtag tttatagagg tttaatttct gcttatagga tagctaaaga ctacgaactt
    24961 gatattattc atactcaaac agaattcagt ttaggacttt taggaaaatt agttgcaaaa
    25021 gctttaagaa tacctgttgt gcatacttat catactcaat atgaggatta cgttggttat
    25081 atcgcaaaag gcaaattgat taagccaagt atggtcaaat atatcatgcg tacctaccta
    25141 agtgatttgg atggtgtcat atgtccttct cgaattgttc tcaatcttct agatggttac
    25201 ggtgtcaaaa ttccaaaaca agttattccg acaggtattc ctgttgagaa ctatcgtcga
    25261 gaggatatct ctgaagaaac aatcaagaat ctacgaacgg agcttggcct tgcggataat
    25321 gatacaatgc tattgagttt atcacgggta tcttttgaaa aaaatattca agcaatcttg
    25381 atgcatttat cagctgtcgt tgatgaaaat cctcacgtaa aattagtgat agttggagat
    25441 ggtccttatc tttcagatct aaaagaactt gttcactctt tagaacttga aaattcagtt
    25501 atttttacag gtatggttga gcatagccaa gtggctatct actataaagc atgtgatttc
    25561 tttattagtg cttcaacaag tgaaacgcaa ggattaactt acattgagag tctggcgagt
    25621 ggacgtccaa tcattgcgca aagcaatcct tatctagatg atgttatttc agataaaatg
    25681 tttggaactc tgtacaagaa ggaaagtgat ttggcagatg ctatcctaga tgcaatagca
    25741 gagacaccca aaatgactca agaagcttat gaacagaaat tatatgagat ttctgctgag
    25801 aatttcagca agtctgtata tgctttttac ctagactttc tgatttcaca aaaagctagt
    25861 gtgaaagaaa aagtctctct gacgatagga aataaggata gccattctac tcttcgtttt
    25921 gtcagaaaag cggtatattt acctaagaaa gtttttacct ttacaggaag agcctctaaa
    25981 aaagtggtaa aagcacctaa acgtcgtatt tcatctattc gagatttttt agattgagac
    26041 gaataaaatt agtaaatgtc ttgaaattat aaaaaatatt gttataataa aaaatgaaga
    26101 catgttaggt tctaatagat ttcaagagcg agcactggtt ggtggaaaga gtgcaattag
    26161 agaacttagt actacttcct tgattttatg taaacataaa agggtggttc ccttatcgcc
    26221 actagaggtc tgacacaagt atactcgagt ttttgtgttg gataaattga ggtggaacca
    26281 cgtagtcttt acgtcctttt ggatgtaaag gctctttttt tataaaagaa gtggttgcat
    26341 aaaagaggca gattgctttt ctaatgttat acttattatc cctatctagg ataatgtgga
    26401 attcaatcaa aggtcaatcc ctttgtttat tagtgaatta atgtgtttag gaattggtat
    26461 caccctaaca gttattaaga aaaggagaac aagatgatta aaatcacttt cccagatggt
    26521 gcaattcgtg aatttgaatc tggcatcaca acatttgaaa ttgcacaatc aatttctaac
    26581 tctttagcta aaaaagcttt agctggtaaa tttaatggtc aacttattga tacaacacgt
    26641 gctatcgaag aagatggtag tattgagatt gtaacacctg accacgaaga tgctttagga
    26701 gttcttcgtc actcagcagc gcatttattt gctcaagctg caaaacgcct ttttccagac
    26761 ttatgtttag gtgtaggtcc agctattcac gatggttttt actacgatac tgataataaa
    26821 tcaggtcaaa tttcaaatga tgatcttcct cgtattgaag aagaaatgaa aaaaattgtt
    26881 aaggaaaatc acccttgtat tcgtgaagaa atcagtaaag aagaagcgtt agaacttttt
    26941 aaagatgacc cttataaagt tgaattaatt agtgaacatg ctgaagatgg tttaactgtt
    27001 tatcgtcaag gtgaatttgt agacctttgt cgtggacctc atgtcccatc aacaggacgt
    27061 attcaagtct ttcatcttct aaatgtcgct ggggcttact ggcgtggtaa cagtgataat
    27121 gctatgatgc agcgtgtcta tggtactgct tggtttgata aaaaagattt gaaagcatat
    27181 cttaaacgcc gtgaagaagc taaggaacgt gaccaccgca aattaggtaa agaacttgat
    27241 ttatttatgg ttaatcctga agtaggtcaa ggattgccat tctggttgcc aaatggagca
    27301 actattcgcc gcgaattgga acgttacatt gttgataagg agattgcttc aggttatcaa
    27361 cacgtttaca cacctccgat ggcatctgtt gaattttaca agacttctgg gcactgggac
    27421 cactatcgtg aagatatgtt cccaacaatg gatatgggag atggggagga attcgttctt
    27481 cgtccaatga actgcccaca ccatattgaa gtttataaac atcatgttca ctcatatcgt
    27541 gaattgccaa ttcgtatcgc tgaacttggt atgatgcacc gttatgaaaa atcaggggcc
    27601 ctcacaggat tgcaacgtgt acgcgaaatg acactcaacg atgcccatat tttcgttaca
    27661 cctgagcaaa ttaaagatga atttcttaaa gctcttaatc tcatagcaga aatttatgaa
    27721 gattttaact taacagatta ccgtttccgc ttatcttatc gtgacccaga agataaacat
    27781 aagtattatg ataacgatga gatgtgggaa aatgcacaag ctatgctaaa agaggctatg
    27841 gatgattttg gattagatta ttttgaagca gaaggcgaag cagcctttta cggtcctaaa
    27901 ttggatatcc aggtgaaaac tgctcttggt aatgaagaaa cattatctac tatccaacta
    27961 gatttccttc ttccagaacg ctttgatctt aaatacatcg gtgcagatgg tgaagaacat
    28021 cgtccaatta tgattcaccg cggtggtatt tcaacaatgg agcgatttac agctatttta
    28081 attgaaactt acaaaggtgc ctttccaact tggcttgctc cacaacaagt ttctgttatt
    28141 ccaatttcaa atgaagccca cattgattat gcttgggaag tggctcgtgc ccttaaagat
    28201 cgtggtattc gtgctgaagt ggatgaccgt aacgaaaaga tgcaatataa gattcgtgcg
    28261 gcgcaaaccc aaaaaattcc atatcaattg attgttggtg ataaggaaat ggaagaaaaa
    28321 gccgtaaatg tgcgtcgcta tggaagtaaa gcgactgaaa caaaatctat agaggaattt
    28381 gtagaaagta ttttagctga tattgcaaga aaatctagac ctgacgaagt taaataaatt
    28441 ttattagtca ttataaaaat cattaata
//
LOCUS       DATRQA010000008        26227 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.008, whole genome shotgun sequence.
ACCESSION   DATRQA010000008 DATRQA010000000
VERSION     DATRQA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26227
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.008"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            8..376
                     /locus_tag="VBD27_000263"
     CDS             8..376
                     /locus_tag="VBD27_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HEO7024305.1"
                     /translation="MLLILLLTVLSGGGYMVFKQHTEKEKMIAIAHSAEAMQIYEKHM
                     KANDSRAFSEQGIIHNYKINDSELNYNPMGGMEVTVYVNNDTNKYFQFTILDNGNGKL
                     ESGSYIISADLDNDLETNKK"
     gene            413..1630
                     /locus_tag="VBD27_000264"
     CDS             413..1630
                     /locus_tag="VBD27_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7024306.1"
                     /translation="MSEYKTIHPNARLKDQYDNTIGVVSEVYDNTSHSGEQIYAVVDN
                     RHIPKEDVKEVTVLFRGSTSPQEFINNPADVTLDWLENDIPMASNIWVMKDFGNPHNF
                     SAVSPQLKASSEHLKEIMKKYPNAKINIGGHSLGGMDAQYAVVDITDKKDLKRINSVH
                     VYNAPDIYPTLTKGQKKTADSLKSKIVVYVDPNDFIGMVGREGKKGSEDSVGTVYYTE
                     SPDINWIDQHMTYGYRMENGQIKVIETNLPPEVRTTRKAMGSFYKDKKKFKKSGKGLS
                     SHEKIFLDAEQAAVIASGLVSTAETALEDIKTSAKTAVGEAEELWNTTKSIPFGITEL
                     TEAELAEAYAEGGVTYDSIVTKTDTHFDEKVKNAGDLVTTYTTLKSDIQSGVETMLSK
                     DSELAGDFKAWEN"
     gene            1618..2019
                     /locus_tag="VBD27_000265"
     CDS             1618..2019
                     /locus_tag="VBD27_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024307.1"
                     /translation="MGKLDAKDDKLSNSKKDLEKLEDDYHHKTIAISDKFFELEDKRC
                     EFETMLQETYEATSYHLRQDEMINEESFMTMNHIIEAFQSDFDTEYTKEKRRLTALEE
                     ETNQKYSKKRQLLEEKIDHLMSERRDYGNPW"
     gene            2003..2308
                     /locus_tag="VBD27_000266"
     CDS             2003..2308
                     /locus_tag="VBD27_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024308.1"
                     /translation="MGILGDYVLSEASDYISGKKDIKALKKKLDKMLVTGVYAPRIKS
                     KRRSIVSTYDAEITTTATNAKASITAIAGQIDTAIKGQFRTKVETVLDNNSTKYDEI"
     gene            complement(2497..2569)
                     /locus_tag="VBD27_000267"
     tRNA            complement(2497..2569)
                     /locus_tag="VBD27_000267"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2534..2536),aa:Lys,seq:ctt)
     gene            complement(2851..4119)
                     /locus_tag="VBD27_000268"
     CDS             complement(2851..4119)
                     /locus_tag="VBD27_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO7024309.1"
                     /translation="MADNLALRMRPRNINEVIGQQHLVGNGKIIDRMVAANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGNIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKKAIQ
                     LAISDKERGFPFLVTIDDGALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPEAQIHTVTALEAAQRIGFPEARILIANIVVDLALSPKSNSAYL
                     AMDAALADLRRSGNLPIPRHLRDGHYSGSKTLGNARDYKYPHAYPEKWVKQQYLPDKL
                     VGHNYFEANETGKYERALGSNKERIDKLSD"
     gene            complement(4112..4603)
                     /locus_tag="VBD27_000269"
     CDS             complement(4112..4603)
                     /locus_tag="VBD27_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001879696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7024310.1"
                     /translation="MIIRPMQNCDREAVLTLQNTGWTALTSPVYDHKWTESDLEKNLA
                     NGMSFFVAEIDDKIAGVLDFGPYYPFPAGKHVATFGILIAEPYQGQGLGKALLKALLT
                     EAKAQGYIKIAMHVMGNNSRAISLYQKYGFTEEARITKAFFIENHYVDALIFAKDLEN
                     NHG"
     gene            4930..5400
                     /locus_tag="VBD27_000270"
     CDS             4930..5400
                     /locus_tag="VBD27_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEO7024311.1"
                     /translation="MAKFGFLSVLEEELDKHLQYDFAMDWDKKNHTVEVTFILEAQNS
                     SAIETVDDQGEMSSEDIVFEDYVLFYNPVKSRFDAEDYLVTIPYEPKKGLSREFLAYF
                     AETLNEVATEGLSDLMDFLTDDSIEEFGLSWDTDAFENGRAELKETEFYPYPRY"
     gene            5536..5994
                     /locus_tag="VBD27_000271"
     CDS             5536..5994
                     /locus_tag="VBD27_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7024312.1"
                     /translation="MDIFDELFDFSGAKIALFCEGKILTSLRDDFPDLPYAGFWDLPG
                     DGREGDETPLECLFREVSEELNLTLTRNHIDWVKTYRGMLNPDKLSVFMVGHISQKEY
                     DSIVLGDEGQDYKLMSIDEFLSHKKVIPQLQERLRDYLEVEDDYFTKGGS"
     gene            5966..6436
                     /locus_tag="VBD27_000272"
     CDS             5966..6436
                     /locus_tag="VBD27_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7024313.1"
                     /translation="MITLQKAEASDLEKIIAIQRASFKAVYEKYHDQYDPYVEEVEQI
                     RWKLVERPDCFYHFVLVDETIVGFLRLVIKDEEKRAWLGTAAILPQYQGQGYGSAAMA
                     LLEKTYPKLTKWDLCTIAQEKLMVSFYEKCGYHSTHTEPEQEGMDMVYMTKKIE"
     gene            complement(6475..7191)
                     /locus_tag="VBD27_000273"
     CDS             complement(6475..7191)
                     /locus_tag="VBD27_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7024314.1"
                     /translation="MKTVKEVSILSGVSVRTLHHYDKIGLFPPTALSEAGYRLYDDEA
                     LIRLQEILLFRELEFPLKDIKYLLEQAKEERQDLLAQQIKLLEWKRSHLEQVITHAKR
                     LQEKGYAYMNFDVYNKTELEQLQAEAKEKWGQTAAYQEFAQKHASDDFAQISQEMAKI
                     MVQFGQLKTQNVSDESVQMCVKRLQDYISQHFYTCTNEILAGLGQMYQSDDRFSQSID
                     KAGGAGTSEFVSQAIAYYCQ"
     gene            7315..7773
                     /locus_tag="VBD27_000274"
     CDS             7315..7773
                     /locus_tag="VBD27_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HEO7024315.1"
                     /translation="MEIVELLREWYSSFEVKEEKWNEKYEGILIRIEGDGNYKRCRLA
                     HKTASKMGYFTVFWQKDGNGKNIPFYEQDEVEDLIIVIKDGRRKGLFIIPKEVAVSKG
                     ILSSANSQGKMAMRFYSPWCSDLNRTALVTQRWQLNYFIDLSKNNEGVTT"
     gene            7770..8723
                     /locus_tag="VBD27_000275"
     CDS             7770..8723
                     /locus_tag="VBD27_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO7024316.1"
                     /translation="MNTWNELTVHVNREAEEAVSNLLIETGSQGVAISDSADYLGQED
                     RFGELYPEVEQSDMIAITAYYPDTLDIEAVKADLADRLANFEGFGLATGSVNLDSQEL
                     VEEDWADNWKKYYEPARITHDLTIVPSWTDYEAKAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKDIYAYDLDDVAVRVAQENIDMNP
                     GTDNIHVAAGDLLKGVQQEADVIIANILADILIHLTDDAYRLVKDEGYLIMSGIISEK
                     WDMVRESAEKAGFFLETHMVQGEWNACVFKKTDDISGVIGG"
     gene            8723..9463
                     /locus_tag="VBD27_000276"
     CDS             8723..9463
                     /locus_tag="VBD27_000276"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEO7024317.1"
                     /translation="MQQYFVNGKADDFVTIEDKDTIKHMFNVMRLTEDDQVVLVFDDA
                     IKRLAKVVDSSAHRFQILEELDSNVEMPVQVTIASGFPKGDKLDFVTQKATELGAAAI
                     WGFPADWSVVKWDGKKLAKKEDKLAKIALGAAEQSKRNRLPQVRLFEKKADFQAELAG
                     FDKIFIAYEESAKEGEMSALAQNLQTVKAGDKLLFIFGPEGGISPKEIAAFEEVGAIK
                     VGLGPRIMRTETAPLYALSVISYSAELL"
     gene            9460..10046
                     /locus_tag="VBD27_000277"
                     /pseudo
     CDS             9460..10046
                     /locus_tag="VBD27_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
     gene            10247..11128
                     /locus_tag="VBD27_000278"
     CDS             10247..11128
                     /locus_tag="VBD27_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEO7024318.1"
                     /translation="MKKHKMLSLLAVSGLMGIGILAGCSNDSSSSSKGTINIVSREEG
                     SGTRGAFIELFGIESKNKKGEKVDHTSDAATVTNSTSVMLTTVSKDPSAIGYSSLGSL
                     NSSVKVLKIDGKNATVKDIKSGSYKISRPFNIVTKEGKEKEATKDFIDYILSKDGQAV
                     VEKNGYIPLDNAKAYQAKVSSGKVVIAGSSSVTPVMEKIKEAYHKVNTKVDVEIQQSD
                     SSTGITSAIDGSADIGMASRELDKTESSKGVKATVIATDGIAVVVNKKNKVNDLSTKQ
                     VKDIFTGKTTSWSDLSK"
     gene            11143..11988
                     /gene="pstC"
                     /locus_tag="VBD27_000279"
     CDS             11143..11988
                     /gene="pstC"
                     /locus_tag="VBD27_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO7024319.1"
                     /translation="MKQTKEGLMQAVFFITACVSVISAILICLFLFSSGLPAITKIGW
                     GNFIFGKVWHPSNNIFGIFPMIVGSLYVTAGALLLGGPIGILTAVFMAYFCPENIYRP
                     LKSAINLMAGIPSVVYGFFGLVVIVPMIRQYIGGFGMGVLAASILLGIMILPTIVSIS
                     ESSLRAVPESYYEGGIALGASHERSVFFAVLPAAKRGILASVVLGIGRAIGETMAVIM
                     VAGNQAVLPQSLTSGVRTLTTNIVMEMGYSSGLHRQALIGTAVVLFIFILMINISFSA
                     LQKEE"
     gene            11990..12868
                     /gene="pstA"
                     /locus_tag="VBD27_000280"
     CDS             11990..12868
                     /gene="pstA"
                     /locus_tag="VBD27_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO7024320.1"
                     /translation="MENINQRTSKQYLASLKKDPLSFFLFAIVYLGAILSFATIAFVV
                     IYILVKGLPHVNTGLFAWTYNTQNVSLLPAFINTIFIIALTLLFAVPLGIGGSIYLTE
                     YARRDNPYLKIIRVATETLAGIPSIIYGLFGALFFVKYTHLGLSLISGSLTLSIMILP
                     LIMRTTEEALLSVPDSYREGAFALGAGKLRTIFKIVLPSAMSGIFAGIILAIGRIIGE
                     SAALIFTAGTVAKVVHSVFSSSRTLAVHMYAISGEGLYVDQTYATAVILLLLVIIVNF
                     VSGLVAKRLGSESNSE"
     gene            12861..13610
                     /gene="pstB"
                     /locus_tag="VBD27_000281"
     CDS             12861..13610
                     /gene="pstB"
                     /locus_tag="VBD27_000281"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7024321.1"
                     /translation="MSKLVINNLDLYYGEFHALKDVNLDIEEKEITAFIGPSGCGKST
                     LLKSINRMNDLVKNCKITGDITLEGEDVYRQLDINQLRKKVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIHSKAELDDIVERSLKQAALWDEVKDRLHKSALGMSGGQQQRLCIARAL
                     AIEPDVLLMDEPTSALDPISTAKIEELVIQLKKNYTIVIVTHNMQQAVRISDKTAFFL
                     MGEVVEYNKTSQLFSLPQDERTENYITGRFG"
     gene            13607..14263
                     /gene="phoU"
                     /locus_tag="VBD27_000282"
     CDS             13607..14263
                     /gene="phoU"
                     /locus_tag="VBD27_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO7024322.1"
                     /translation="MIRSRFASQLNDLNKEIIFMGALCEDIIGKSLGALTNSNDVYLD
                     DISETYHKIEQMERDIEERCLKLLLRQQPVAKDLRRISSALKMVYDMKRIGAQAYEIA
                     EIVSLGHIIQGAGSERDQLNSMSNNVISMLTKSIDAFIYDNEEQAHQVIEQDRTVNQE
                     FDTIKKQLVLYFSVQDVDGEYPIDVLMIAKYLERIGDHTVNIAKWVLFSITGNLDGEA
                     "
     gene            14263..14940
                     /locus_tag="VBD27_000283"
     CDS             14263..14940
                     /locus_tag="VBD27_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7024323.1"
                     /translation="MIYCVEDDADIREMMLYTLQMAGFTAQGFSSSELFWEAVQEKVP
                     DLILLDIMLPGDDGLTILERLRRKHQTEMIPVIMTTAKGSEYDKVKGLDLGADDYLVK
                     PFGMMEMISRIKAVLRRSRQVDSKAHIIIGNLEIDPTNYWVKRGTEKIHLTLKEFELL
                     VLFFRNPNRVFTRQELLDKVWGEQFLGETRTVDVHIGTLRTKLGEDGYLIATVRGVGY
                     RLEERHD"
     gene            14933..16588
                     /locus_tag="VBD27_000284"
     CDS             14933..16588
                     /locus_tag="VBD27_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO7024324.1"
                     /translation="MTKKIFRTTLSASLGIVLVTILMIMGFLYNYFNRIQREQLRTQT
                     ALASQGISFEGKDYFENLKTSNVRITWVDNKGQVLYDTQSDAKHMKNHANRQEIKEAI
                     KSGYGESTRWSATLTEKSIYAAQRLNNGTIVRLSVAQQTIFYLLLGMMSPLAIIILLA
                     IILSVLIARYIAKKVSEPLNNIDLDHPLSNDSYEEITPLLRRLDSHQAKIQHQKLLLQ
                     KRQKEFDTIISKIKEGMILLDDQARIVSINAEALKLFQINDDWHGRFMMEVSRDLTLK
                     DLIDQGLKGKKKEANIDIENNHYRVLVRPTTDNNRVTGLVVLLFDVTDQLQMEQLQRE
                     FTANVSHELKTPLHVISGYSELLANQMVPNEEVPQFAAKIHKESERLVKLVEDIINLS
                     HLDEQEKLPQETVNLYDLTQKVLEGLQAKADKKHIQINFNGEEAILRGNPVLLNSLVY
                     NLCDNAITYNHEKGQVNVTLKNSPDTITLEVSDTGLGIAEKDKKRIFERFYRVDKSRS
                     KIVGGTGLGLSIVKSALDFHNGSIKVDSHLGQGTTMTVLLHKQ"
     gene            16743..18926
                     /locus_tag="VBD27_000285"
     CDS             16743..18926
                     /locus_tag="VBD27_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO7024325.1"
                     /translation="MKNNVKQLFSFEFWQKFGKALMVVIAVMPAAGLMVSIGNSIRLL
                     DPSNVLLGRIANVIAQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLSFILINLI
                     TGNFFGVKTDMLANSKATVQTVFGATIRVSDYFVNVLGQPALNMGVFVGIISGFVGAT
                     AFNKYYNYRKLPDALTFFNGKRFVPFVVIYRSVIVALILSVFWPVVQSGINGFGKWIA
                     SSQDSAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTQLGGTYTVLTGATKGAQVLGQ
                     DPLWLAWVGDLINLKGSNSSQYHHLLTSVTPARFKVGQMIGASGILMGLSYAMYRNVD
                     KDKKLKYKSMFISAAAATFLTGVTEPIEYMFMFAAMPLYLVYAVVQGCAFAMADIVNL
                     RVHSFGNIEFLTRVPMGIKAGLGGDIFNFVWVTLLFAVLMYFIANFMIKKFNLATAGR
                     NGNYDNEEVDNAPSTASGSADANSQVVQVINLLGGRDNIEDVDACMTRLRVTVKDGNS
                     VGSEAAWKKAGAMGLVLKGNGVQAIYGPKADVLKSDIQDLLDSGTVIPIVDLETGQPV
                     AATQVTTYKGITEEIVSVANGQVEALDVVKDPVFSQKMMGDGFAVEPTDGNIYVPVSG
                     TVTSVFPTKHAFGLLTESGLEVLVHIGLDTVALDGQPFEVKISSGQKVVAGDLAVVAD
                     LEAIKAAGKETSVIIVFTNVSDIKTVKLEKSGPQIAKTVVAKVEL"
     gene            18965..19795
                     /locus_tag="VBD27_000286"
     CDS             18965..19795
                     /locus_tag="VBD27_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO7024326.1"
                     /translation="MKGYSMAKCLTLNTHSWMEVNALKKLFDLAEHIFREKYDIICLQ
                     EVNQSISSPLATSSPNYHPIEGTPALHQDNFALQLVHYLNLQGLHYHWTWAYNHIGYS
                     KYHEGVAILSLKPLKPDDILVSAVDDETDYHTRRALVAETTLNDKVVTVVSLHLSWFE
                     KGFAEEWKRLETTLLEVETPLLLMGDFNNPTGNQGYELVLNSPLALKDSHQIANHVFG
                     DHTIMADIDGWEGNKKALKVDHIFTSEDLSISSSQVVFEGGEAPVVSDHYGLEITLNL
                     "
     gene            20011..20763
                     /locus_tag="VBD27_000287"
                     /pseudo
     CDS             20011..20763
                     /locus_tag="VBD27_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645527.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            20760..22094
                     /locus_tag="VBD27_000288"
     CDS             20760..22094
                     /locus_tag="VBD27_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO7024327.1"
                     /translation="MNSTLSILEIALTIILTIFLFSKISGRQLTLIEIGICIALRLGL
                     VILFIAANTIINLSFLKYWLLPIYMLSLAFLLLRPISKTLVVFYGLFPIVLRNLCSRS
                     LAFFVFPLFNIDFHTTNQGFWGYFVDVLSTLCVLLFLRWMQYDFKTLRSSEVSPIDKR
                     ILQFTNGCMVSYYLLMQFSTYLEYELAINTVIYRQFLLVVYWIIFTGAIASLDRRLRN
                     SLQERLAVQRELQLANMIDYSHHIEGLYKEVRSFRHDYSNMLTTLKLGIDNEDISIVK
                     SVYQSIFKESNTHFRHPKYNIDRLVNVDNPALKSLLAAKLSQAIDNHIMVSIEVPDSI
                     KPQGMELIDFITIVSVLWDNAIEASLETDDPKIAMSYFTLKHKQIFIIENSTKEKAID
                     TSTIFNYEISSKGRNRGIGLYNVRGVINRYPNISIATSSQDFSFCQTFEIIL"
     gene            complement(22183..22347)
                     /locus_tag="VBD27_000289"
                     /pseudo
     CDS             complement(22183..22347)
                     /locus_tag="VBD27_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632309.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            22636..22809
                     /locus_tag="VBD27_000290"
     CDS             22636..22809
                     /locus_tag="VBD27_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO7024328.1"
                     /translation="MKHYHMLKVALVIDGYTVRTGKHTYTSAWHLSENTAAANARNRM
                     GLYPGSVCRVYLV"
     gene            22878..23726
                     /locus_tag="VBD27_000291"
     CDS             22878..23726
                     /locus_tag="VBD27_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7024329.1"
                     /translation="MLLQLDNITKSYGKKIVLNQISYQFTPGLYGLLGANGTGKTTLL
                     NLMSHFTFADSGNIYWNGQEQSEEFYRHIGFLPQHFRYYDQFTGIAFLNYIATLKGVD
                     KKKAKPEIPRLLELVGLGDVGKKKISSYSGGMKQRLGIAQALINDPEILILDEPTVGL
                     DPKERVKFRHILSQLSTNKIIILSTHIVSDVEAVAKEIIVLKNGKFIEQGNTAQLLKT
                     IEGKVWEITTEPDLSQIPNIAIVNEKVISDSLVFRVVSDICPSDSAQLVVPTLEDFYI
                     YHFRGE"
     gene            23732..24886
                     /locus_tag="VBD27_000292"
     CDS             23732..24886
                     /locus_tag="VBD27_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO7024330.1"
                     /translation="MISIFPFELKKLLQRKAALGAFIIFLLAIFGLFYQHFFVGQISG
                     YSADKIHGREAVAINRRIAEKYSGDLSEERVTNIIKNYVNPPAGRPPIFDVFSYYVAD
                     TFDRDFLIYYNQLKDDKTIDFNKINIKDQKQLGSALPLKQLKLGNFAPWNQLFEVSNS
                     TYILIVLFTLFLSAPLFSGDRAKKINPILLTTRYGRGQLTWAKLASTMTIGISTFILA
                     YALILTVFADYFGFSGWETSVQLNLYWTSPLANIMGFPVPMTISTAFVHIIVLQFVGL
                     LFIASLNFFILSITNSPLTSFAVSTLAFFAPSFLMAMFNKGVINKLLTIFSVSTTDTS
                     ALLLKLSRNGSHGFFFDSFSANEICLISLRMLIAVLLIAYTYRLMRRRSL"
     gene            25115..26149
                     /locus_tag="VBD27_000293"
     CDS             25115..26149
                     /locus_tag="VBD27_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO7024331.1"
                     /translation="MDNKQHNTSMISLLQYLFSILVILVHSGRLFSQDVIHFTFKSFL
                     GRMAVPYFLICTAFFLRGRIQQGLCNHSYFRKLIKKYSMWTIIYLPYGYFFFESLNIA
                     KIYLLPGFIVAFLYLGMSHTLWYIPAVILGWFIIQGLLKYVGTRGTFITVVVLYCIGA
                     VETYSVFVQSTKFYPLMSTYMSIFQTTRNGLFYTPVYLLAGYLLYDYFNTDLFTKSRG
                     LKYILFLLLLALENVLIYFNQGLDKNFFLLAPLCAVFLFNWSIRTSLFKGYRLSPLKQ
                     LSVYYFFLHPLFVGIVSYCLKSTSLVAHHQGKVIFVVTLALTHACALLIIKVVNKIPY
                     QKIIPLSLSK"
BASE COUNT         8291 a         4183 c         5337 g         8416 t
ORIGIN      
        1 atattggata ttactaatat tactattaac agttttatca ggaggcggat atatggtttt
       61 taaacaacat acagaaaaag aaaaaatgat tgcaattgca cacagtgcag aagcgatgca
      121 aatttatgag aagcatatga aagcaaatga ttcgcgagct ttttcagagc aaggaattat
      181 tcataattat aaaattaacg attcagaact caattataat cctatgggag gaatggaggt
      241 tactgtttat gtgaataatg ataccaataa atattttcaa tttactattt tagataatgg
      301 aaatggtaaa cttgaatctg ggagctatat tataagtgca gatttagata atgatttaga
      361 aaccaataaa aaatgaaaaa aatatattga tgaagaaaaa ttacaaatcg caatgtcaga
      421 atataaaaca atccatccca acgccagatt aaaggatcaa tatgataaca caattggagt
      481 tgtttcggag gtttacgaca atacttctca ttctggagaa cagatttatg ctgtggttga
      541 taatcgtcat attccaaaag aagatgtaaa agaggtgacg gtactttttc gtggatcaac
      601 atctcctcag gaatttatta ataatcctgc agatgtgact ttggattggt tagaaaatga
      661 cattcctatg gcaagtaata tttgggtaat gaaagatttt gggaatcctc ataatttcag
      721 tgctgtatca ccacagttga aagcttcatc agaacattta aaagagatta tgaaaaaata
      781 tccaaatgca aaaataaaca ttggtggaca ttcattagga ggtatggatg ctcaatatgc
      841 tgttgtagat attacagata agaaagactt gaaacggatt aactctgtac atgtttataa
      901 tgctccagat atatatccta cacttactaa aggacagaaa aagacggcag attcactgaa
      961 atctaagatt gttgtttatg ttgatcctaa tgattttatt ggcatggtgg ggcgtgaagg
     1021 aaaaaaagga agtgaagact ctgtaggaac agtctattat acagaaagtc cagatatcaa
     1081 ctggatagat caacacatga cctatggcta tcgtatggaa aatggtcaga ttaaagttat
     1141 tgaaacgaat ttaccaccgg aagttaggac tactagaaaa gctatgggat ctttttataa
     1201 ggacaagaaa aaatttaaaa aatctggtaa gggtctaagt tcgcatgaaa aaatcttctt
     1261 ggatgctgaa caagctgctg ttattgcaag tggtttagtc tctactgctg aaactgcttt
     1321 agaggatatc aaaacatctg cgaaaacagc tgttggagaa gcagaagaat tgtggaatac
     1381 gactaaatcg attccttttg gtattacgga gcttacagaa gcagagttgg cagaagctta
     1441 tgcagaaggt ggtgtcactt atgatagcat agtaactaaa actgatactc attttgatga
     1501 gaaagtcaaa aatgcaggag atttagtaac aacatatacg actttgaaaa gtgatattca
     1561 atcaggtgtt gaaaccatgt tatctaagga tagtgaatta gctggagatt ttaaagcatg
     1621 ggaaaattag acgctaaaga tgataaatta agtaacagta aaaaggattt agaaaagttg
     1681 gaagatgatt accatcataa aaccatagct atttcagata aattctttga actggaagat
     1741 aaacgatgtg agtttgagac gatgcttcag gaaacttacg aagcaacaag ttatcatctt
     1801 cgtcaggatg agatgattaa tgaagagtcc tttatgacca tgaatcacat tatagaagct
     1861 tttcaaagtg attttgatac agaatatact aaagagaaaa gaagattgac tgccttagaa
     1921 gaagaaacta atcaaaagta ctctaaaaag agacaattgc ttgaagaaaa aatagatcat
     1981 ttaatgtcag aaaggagaga ctatgggaat ccttggtgat tatgttttat cagaagcctc
     2041 tgattatatc agtggaaaaa aagatatcaa ggctttaaaa aagaaacttg ataaaatgtt
     2101 agtgacaggt gtttatgctc caagaatcaa gtctaaacgt agaagtattg tctctaccta
     2161 tgatgcagag ataacgacta ctgcaacaaa cgcaaaagct agtataactg ccattgcagg
     2221 tcaaatagac actgcaataa agggacagtt tagaactaaa gttgaaactg tattggataa
     2281 taacagtaca aagtatgatg aaatttaaaa gacatgggct aaataattta gctctttttt
     2341 tatactcaaa aataagagaa atacaaaaca aaatacatat ttatgcaagt aaagagaaat
     2401 aaaaaaatca ccattttatc tgtcaaaaaa aattggtggg caagtgtaaa aagagcgtca
     2461 aaaaagccta taggtatagg ctttgtttta taagactgcc ccctacaggg ctcgaacctg
     2521 tgacccatag attaagagtc tactgctcta ccaactgagc taaggaggct aggaagctgt
     2581 attggcaccg atgatccact ttttgtattg atcccgcaca aaataagcag gtgggtaact
     2641 cgcacctctt taagtggctt ccgcgtgtta acggcttgcg cactagagat gacttcgttt
     2701 cccgaaatac aaagaaatag tcggtcaaca cttagatgtg gattcgtatg ccacagcaat
     2761 gtttctatgt ctttatgata ccacttttta taaaattttc aagcaaaatt atcgattttt
     2821 gaaatcgatt gcgattttta aaactggcaa tcagtctgag agcttatcaa tgcgctcttt
     2881 atttgaacca agtgcacgtt cgtatttacc agtttcattg gcttcaaaat aattatgtcc
     2941 tactagttta tctggtaagt attgttgttt aacccatttt tcaggatagg cgtgagggta
     3001 cttgtaatcc cttgcattgc ccaaggtttt actaccgctg taatgtccat ctcttaggtg
     3061 acgtggaata ggtaaatttc ctgaacgacg taaatcagct aaggctgcat ccatagccaa
     3121 ataggctgaa tttgatttag gtgatagagc taaatccaca acaatgttag caataagaat
     3181 acgagcttct ggaaaaccaa tgcgttgggc agcttccaat gcagtcacag tatgtatttg
     3241 tgcttcgggg ttagctaagc cgatatcttc ataagcaata atggtcaaac gtcttgctaa
     3301 actaggtaaa tctccagctt ctactaaacg agcggcatag tgcaggctgg cattgacatc
     3361 agaacctcta attgattttt gtaaggctga taggacatca taatgcccat ctccgttttt
     3421 atccattgta atataactac gttgcagact attttccatt gtctctagac taatatgacg
     3481 gctaccatct tcgttaggag aagtagacat gactgctaga tccaaggaat tataagcaga
     3541 tcgtaggtct ccatttgtgg cggtaacaat gaaatctagc gccccatcat caattgtcac
     3601 taaaaaagga aatccccgtt ctttatcgga aattgctagt tgaatggctt ttttaatatc
     3661 ctcgttagaa agaggttcta gctcaaaaat ttgtacccta cttctaatgg caggagttac
     3721 tgaaaagaaa ggattttcag ttgttgctcc aatcataata atattgccat tctcaagtaa
     3781 aggtaaaaga aaatcttgtt ttgtcttgtc aagtctatga atctcgtcaa gtaataaaac
     3841 taagccacca gaaaatttag cttcttctgc aatttcttga agacgttttt tactatcaac
     3901 agtggcatta aaagtacgaa aggcatattt agtagtacca gcaatagcag aagcaataga
     3961 agtcttccca atacctggtg gtccatatag gatcatggaa gataacatat tagctgcaac
     4021 cattcgatca ataattttgc cattaccaac gagatgttgc tgcccaatga cttcattaat
     4081 attgcgagga cgcatacgta gagcaagatt gtcagccatg attattctcc aaatcttttg
     4141 cgaaaattaa agcatcaaca taatgatttt caatgaaaaa ggctttagtg attcgagcct
     4201 cttcggtaaa accgtatttt tgatagaggg aaatggctct actattgttg cccatgacgt
     4261 gcatggcaat cttgatgtaa ccttgagctt tagcctcagt tagcagagct ttcaagagag
     4321 ccttacctag cccctgacct tggtagggtt cagcgatgag aatgccgaaa gtggcaacat
     4381 gcttgccagc tgggaaagga tagtagggac caaagtccaa gacgccagcg atcttatcat
     4441 cgatctcagc gacaaagaag gacatgccat ttgccagatt cttctccaag tcactctcag
     4501 tccacttgtg gtcatagaca ggagaggtaa gagcagtcca gccagtattt tgtaaggtca
     4561 gaactgcctc acggtcgcag ttctgcatag gtctgataat cataattgat gatacaaagg
     4621 gacggttcgg tttacgaaaa ttttcgtaaa gaaataggga acgacgaagt gtcgttagac
     4681 actaggaagt tcatcttttc ttttagaaaa ttagtcccgt gttcaaatcc tttctatttt
     4741 ttatatagag tttttctctc agctgactct tttaacatag tatgaagtca gtcaaaaacg
     4801 actagtcttt gagactattg taacataaaa ataagtagga actacctttt gattaacctg
     4861 agtctgtaac gtttttcttc tatattcatg ttataataag aaagaataaa tagaaaataa
     4921 ggacataaga tggctaaatt tggattttta tcggtactag aagaagaatt ggataagcat
     4981 ttacagtatg actttgccat ggattgggat aagaaaaatc atactgtaga agtcactttt
     5041 atattagaag cacaaaacag ctctgctatt gaaaccgtgg acgaccaggg ggagatgagt
     5101 agcgaggaca tcgtttttga ggactatgta ctcttttaca accctgtcaa gtctcgcttt
     5161 gatgctgagg actatctggt gactatcccc tatgagccta agaagggctt gtcgcgtgag
     5221 tttctggctt attttgcgga gactctcaac gaggttgcca ctgaagggtt gagcgacctc
     5281 atggacttcc tcaccgatga cagtatcgaa gagttcggtc tttcttggga tacggatgct
     5341 tttgaaaatg gcagagcaga actgaaagag acagagtttt acccttatcc gagatattag
     5401 acttgatgga tttgctaaaa tgtggatata cattggatga tatagaaaca gccagaccag
     5461 aggatatgga acgttattat ccccctgaac aaatcagaaa atatggagct ttaggaatag
     5521 aattgaggtt attgcatgga tatttttgat gagttatttg atttctcagg tgcaaagatc
     5581 gctcttttct gcgagggaaa gattttgacc agcctaagag atgattttcc agatttgccc
     5641 tatgcaggct tttgggactt accaggcgat ggtcgtgagg gcgatgaaac acctttggaa
     5701 tgtctttttc gagaagttag tgaagaatta aatctgacct taactcgaaa ccatattgat
     5761 tgggtcaaga cttatagggg tatgctgaac cctgataaat tatcggtttt tatggttgga
     5821 catatcagtc agaaagagta cgatagcatt gttctaggcg atgaaggaca agactacaag
     5881 ctcatgtcca ttgacgaatt tttaagccat aaaaaggtca ttcctcagtt gcaggagagg
     5941 ttgagagatt atttggaggt ggaagatgat tactttacaa aaggcggaag ctagtgattt
     6001 agagaagatt attgccattc agagagctag ctttaaggca gtctatgaga aataccacga
     6061 tcaatacgat ccctatgtgg aagaagttga gcagattcga tggaaactag tggagcgacc
     6121 agattgtttc tatcattttg tccttgttga tgagacaatt gttggttttc ttagattggt
     6181 tatcaaggac gaggagaagc gtgcttggct tggtacggcg gctattcttc cccaatatca
     6241 aggacagggc tacggctcag cagcgatggc tttattagag aaaacctatc ctaagcttac
     6301 aaagtgggat ttatgtacga ttgcgcaaga aaaactaatg gtcagttttt atgaaaaatg
     6361 tggttatcat tcaacccata ccgaacctga gcaagaagga atggatatgg tctatatgac
     6421 aaagaagata gaataaaaaa agaaaactag atgattgtct agttttctga tagtttattg
     6481 gcaatagtaa gcaatagctt ggctgacaaa ttcacttgtt cctgctccac cagctttgtc
     6541 aattgattga ctaaaccggt catctgattg atacatttgt cctagtccag caaggatttc
     6601 attggtacag gtgtaaaaat gctgagagat atagtcttgt agccgtttca cacacatttg
     6661 aacagattca tcagatacat tttgtgtctt taattgccca aattgtacca ttatttttgc
     6721 catttcttgg ctaatttgtg caaagtcatc tgaagcatgt ttttgagcaa attcttggta
     6781 ggctgctgtt tgtccccatt tttcttttgc ttcagcttgt aattgttcta attctgtttt
     6841 attataaaca tcgaaattca tataagcata tcctttttct tgtaatcgtt tcgcatgggt
     6901 gataacctgc tcaagatgcg atcttttcca ttcaagcaat ttgatttgtt gggctaataa
     6961 gtcttgcctt tcctcttttg cttgctcaag caggtatttg atatctttga gagggaattc
     7021 caactcacga aatagcaaaa tttcttgcag gcgaatcaat gcttcatcgt cataaagtcg
     7081 atagcctgct tcggataggg cggtaggtgg gaataaaccg attttatcat agtgatgtaa
     7141 ggtgcgcaca cttacaccgc ttaagatact gacttcttta actgttttca tggtaataac
     7201 ctcctctcac ttacatagta taaaggataa cctaaggtca gggtcaagca aaaaataaat
     7261 tattttgaaa agtatggtta atcatattga tttgaagata aaggagggat agttatagaa
     7321 atagttgagc tactaaggga atggtattct tcctttgaag ttaaagaaga aaagtggaat
     7381 gaaaaatatg aaggtatcct cataagaatt gaaggcgatg gaaactataa aaggtgtcgt
     7441 ttagctcata agacagctag caagatgggg tattttacag tattttggca gaaagatgga
     7501 aatggtaaaa atattccttt ttatgaacaa gatgaagtgg aagatttgat tattgtcatt
     7561 aaagatggta ggaggaaagg tttgttcatc attcccaaag aggttgcagt gagtaagggt
     7621 attctttcat ctgctaactc tcaaggaaag atggctatgc gattttactc tccttggtgt
     7681 agcgatttaa atagaacagc tttggtgaca cagcgatggc agttgaacta ttttattgat
     7741 ttaagtaaaa ataatgaagg agttactaca tgaacacatg gaatgaacta acggttcacg
     7801 tgaatcgtga ggcagaggaa gctgtctcga atttattgat tgaaactggc agtcaggggg
     7861 ttgccattag cgactcggct gattatcttg gacaggagga tcgtttcggc gagctttatc
     7921 ctgaggtgga gcaatctgat atgatcgcta ttactgccta ttatcctgat acgcttgata
     7981 ttgaggcggt taaggctgat ctggctgatc gtttggctaa ttttgaaggc tttggtcttg
     8041 cgacgggtag cgtcaacctt gacagtcagg aattggttga agaggactgg gctgataact
     8101 ggaagaagta ctatgagcca gctcgcatta cccatgattt gaccatcgtg ccgtcatgga
     8161 cggactacga ggctaaggct ggtgagaaga ttatcaagct ggatcctggt atggcttttg
     8221 ggactgggac tcacccaacg actaagatga gtctttttgc cttggagcaa gtgcttcgtg
     8281 gtggtgagac ggttatcgat gtggggactg gttctggcgt tttgtctatt gctagctctc
     8341 tcttgggtgc caaggacatc tatgcttatg atttggatga tgttgcggtg cgcgtggctc
     8401 aggaaaatat cgatatgaac cctggtactg ataatattca tgtggctgct ggtgacctgc
     8461 tcaaaggtgt ccagcaagag gcagatgtca ttattgccaa tattttggcg gatattttga
     8521 tccatctgac agacgatgcc taccgtttgg tcaaggatga gggttatctc atcatgtctg
     8581 gtatcatttc tgaaaaatgg gacatggtgc gtgagagtgc tgaaaaagct ggtttcttcc
     8641 ttgagactca catggttcaa ggtgagtgga atgcctgtgt tttcaaaaag acagatgata
     8701 tttcaggagt gattggcggc taatgcaaca gtattttgta aatggcaaag cagatgactt
     8761 tgtgaccatc gaggacaagg ataccatcaa gcacatgttc aatgtcatgc gattgacaga
     8821 agatgaccaa gtggtcttgg tttttgatga tgccatcaag cgtctggcaa aggtggtgga
     8881 cagctctgcc catcgcttcc aaattctaga agagctagac agcaatgttg aaatgcctgt
     8941 ccaagtgacc atcgcctcag gttttcccaa gggagacaag ttggattttg tgacgcaaaa
     9001 ggcaacagaa ttgggtgctg cggctatctg gggattccca gctgactggt cagtcgtcaa
     9061 gtgggacggc aagaaactag ctaaaaaaga agataagctg gcaaaaattg ctctgggcgc
     9121 agcggagcag agcaagcgta atcgtcttcc tcaggttcgt ttatttgaga agaaggctga
     9181 ttttcaagcg gagcttgcag gctttgataa gatttttatc gcctatgaag agtctgccaa
     9241 ggagggtgaa atgtcagcgt tagcccagaa tttgcagact gtaaaagcag gtgacaagct
     9301 cctcttcatc tttggaccag aagggggcat ttctccaaaa gaaatcgcag cttttgaaga
     9361 ggttggagct atcaaggtgg gcctgggtcc acgcatcatg cgcacagaaa cagcaccgct
     9421 ctatgctcta agtgttatta gttactcagc ggagttatta tgattattgc tattcaaggt
     9481 gatatggctg tagggaaaac cacagctttg aaaatcattg agaaagttta cccgaaaatc
     9541 gctattagtt ttgaggatat ttcaccagta gttgataaga ttaataaaca tcagcttgat
     9601 aacaataaat tagacgatta ttgtgaaatt caacaactat ttattcagca tgagcgagat
     9661 agatacgaac ttgttagaga taatgaaata gtgttaatgg attatggagc tgaggaaatt
     9721 gctttctata cacaatactt tccaaaggtg gcgggttata gttgacttta ccttctaatt
     9781 tgttactaga ggtaaatagg ctaaaggaat gttttgctga ttatattatt tttctagatg
     9841 cagatgagac ggtcctatac caacgtcaaa aaaatgattt taatagacgg cgtcattttt
     9901 tcaaatggta tactcatcac ctgttgcctg ctaaaaaaca ttatttagac acattttcca
     9961 atgttattca tattaataca aatcatttaa atgctgaaga agtggctgaa aaaatcattg
    10021 acatcattcg taagttaact ctataatggg atagttatac ttcaattatg tggtataaca
    10081 actgtttcaa gccgacttat tggctgaatt accataatag aaaaaaggag tttggataat
    10141 tttccaactc tttttaattt tacattgatt ttacaaaaat cctcaaagag atttgatatt
    10201 ccttaactat aataaaatca aatcaaaaaa tatttaggag ttcatcatga aaaaacacaa
    10261 aatgctatct cttttggcgg tatctggtct tatgggaatt ggcatcctag caggatgctc
    10321 aaatgattca tcatcttcat ctaaagggac aattaacatt gtttctagag aggaagggtc
    10381 tggaactcgt ggagctttca tagagttatt tgggatcgag tcaaaaaata aaaagggcga
    10441 aaaagtagac catacttctg atgcagcaac tgttaccaat tcaacttcag ttatgctaac
    10501 cactgtctct aaagacccgt cagctattgg ttattcttca ctaggttcat taaatagtag
    10561 cgttaaagtg cttaaaattg atggcaaaaa tgcgactgta aaagatatta aatcaggaag
    10621 ctataagatt tctcgtcctt tcaatattgt aacaaaagaa ggtaaggaaa aagaagcaac
    10681 aaaagatttt attgattata tcttaagcaa agatgggcaa gcagtcgttg agaaaaatgg
    10741 ctatattcca cttgacaatg ctaaagcata tcaagcaaaa gtgtcatctg gtaaagttgt
    10801 tatagcaggt tcaagttcag taacgccggt tatggaaaag attaaagaag cctatcataa
    10861 agtaaataca aaagtagatg ttgaaattca acaaagtgat tcttcaactg ggattacaag
    10921 cgcaattgat ggatcagcag atataggtat ggcatcacgt gaattggata agacagagtc
    10981 atccaaaggg gtcaaagcta ctgttattgc aacagatggt attgcagtag ttgttaataa
    11041 gaaaaataaa gttaatgatc tatcaacaaa acaagtaaaa gacattttta ctggaaagac
    11101 aacgtcttgg tctgacttat caaagtaagg taagagtaaa caatgaaaca aacaaaagaa
    11161 ggtcttatgc aagctgtgtt tttcatcacg gcttgcgtca gtgttatatc agctatttta
    11221 atttgcctct ttctcttctc tagtggtctt ccagctatta caaaaatcgg ctggggaaat
    11281 tttatatttg gtaaagtctg gcatcctagt aataatattt ttggcatctt tccaatgatt
    11341 gtaggttcct tatatgttac agcgggagct ttacttttag gaggcccgat aggtatcctt
    11401 actgctgttt ttatggctta tttctgccca gaaaatattt ataggccttt aaagtcagct
    11461 atcaatttaa tggcgggaat tccttcagtt gtttatggtt tctttggact cgttgtcatt
    11521 gtccctatga taaggcaata tattggtggc tttggtatgg gagtcttagc agcttcaatt
    11581 ttgctaggaa ttatgatttt accaacaatt gttagcattt cagaatcttc tttaagagct
    11641 gttccagaga gctattatga aggcggtatt gcccttggtg cctctcatga acgaagtgtc
    11701 ttctttgctg ttttaccagc tgctaaacgt ggtattttag catcggttgt tttgggaatt
    11761 ggacgtgcta ttggtgaaac aatggctgtc atcatggtag cgggtaatca agctgtcttg
    11821 cctcaatcgc tgacatcagg cgttagaact ctaaccacta atattgtcat ggaaatggga
    11881 tattcttctg gtttgcatcg tcaagcctta attggtacag ctgtggtact ctttatcttt
    11941 atattaatga ttaatatcag tttttcagca ttacagaaag aggaataaga tggaaaatat
    12001 taaccaaaga acgtcaaaac aatatttagc atctttaaaa aaagatcccc tttccttctt
    12061 tctttttgct attgtctatt tgggagcaat tctaagtttt gctacaattg catttgttgt
    12121 tatttatatt ttagttaagg ggcttcctca tgtgaatact ggcttgtttg catggactta
    12181 taatactcag aacgtctcac tattaccagc tttcattaac actatcttta tcatcgcctt
    12241 aactttatta tttgcagttc cccttggtat tggtggatca atttatctaa cggaatatgc
    12301 tagacgtgat aatccttatc tcaaaattat ccgtgttgca accgagacat tggcagggat
    12361 accttcaatt atttatggtt tatttggtgc attatttttt gtaaaataca cacacttagg
    12421 cttatctttg atatcgggtt ctctgacttt gagcatcatg attttacctt tgattatgag
    12481 gacaactgag gaagctttac tatctgttcc ggattcttat cgagaaggag cctttgcgct
    12541 tggtgctggt aaattgagaa ccatatttaa aatcgtatta cctagtgcta tgtctggtat
    12601 ttttgctggt attattttag cgataggacg tatcattggt gagtcagcag cacttatttt
    12661 cacagctgga acagttgcaa aagtagtaca ttcggtattt agttcatctc gtactttagc
    12721 agtacatatg tatgctattt cgggtgaggg cttatatgtt gaccaaactt atgcgactgc
    12781 ggtcatctta ttgcttttag ttattattgt caattttgta tctggactag tagccaaacg
    12841 attaggaagt gagtcgaaca gtgagtaaat tagtaattaa taatttagat ttgtattatg
    12901 gagaatttca cgctctgaag gatgttaatt tagacattga agaaaaagaa atcacagcct
    12961 ttattggacc ttctgggtgc ggaaagtcta ctctcttgaa aagtattaac cgtatgaatg
    13021 atttggtcaa aaattgcaaa atcacagggg atattacttt agaaggcgag gatgtctata
    13081 gacaattgga tattaaccaa ttgcgtaaaa aggttggaat ggtgtttcaa aaacctaatc
    13141 cgtttccaat gagcatttat gataatgttg cttttggtcc aagaacgcac ggtattcatt
    13201 ccaaagcaga gttagatgat atcgttgaac gttcccttaa acaggcggct ctatgggatg
    13261 aggttaaaga tcgtcttcac aaatctgctc tgggtatgtc tggcggacag cagcaacgtt
    13321 tgtgtattgc gcgtgctctt gctattgagc ctgatgtttt acttatggat gaacccacaa
    13381 gtgccttgga tcctatttca acagcaaaaa ttgaagaatt ggttatccaa ctgaagaaga
    13441 actacactat tgttatagtg acacataata tgcaacaagc tgttcgtatt tctgataaaa
    13501 cagcattctt tctgatggga gaagtggttg aatataacaa aacgagccaa ctcttttcat
    13561 tgccacaaga tgagcgtaca gaaaactaca ttacagggag attcggatga ttcgttcacg
    13621 atttgcaagt caacttaatg accttaataa agaaattatt tttatggggg cattgtgtga
    13681 ggacatcatt gggaagtcct taggtgctct aacaaatagt aatgatgttt accttgacga
    13741 tatttctgaa acttatcaca agattgaaca aatggaacgg gatattgagg agagatgttt
    13801 aaaactttta cttcgtcagc aaccagttgc caaggattta cgccgtattt cttctgcatt
    13861 aaaaatggtt tatgatatga aacgtattgg tgctcaagct tatgaaattg cggaaattgt
    13921 tagtttaggt catattatcc aaggtgcagg ttctgaaagg gatcaactta acagcatgtc
    13981 taataatgtc attagtatgc tgactaagag tatagatgct tttatttatg acaatgaaga
    14041 acaagcgcat caagtaattg aacaagatag gacagttaac caggaatttg atacaataaa
    14101 gaaacagtta gtgttatatt tttcggtaca ggatgttgat ggtgaatatc ccatagatgt
    14161 tttaatgatc gccaagtacc tagagcgtat tggtgatcat acagtaaata ttgctaaatg
    14221 ggtgcttttc tcaataacag gtaacctaga tggagaagcg taatgatata ttgtgttgaa
    14281 gatgatgctg atattcgcga aatgatgctc tatactttgc aaatggcagg ttttacagct
    14341 caaggattct cgagttcaga gcttttttgg gaagctgttc aggagaaagt tccagacttg
    14401 attttacttg atattatgtt gccgggagat gatggtttga ctattttaga gcgtttaagg
    14461 agaaaacatc aaacggaaat gataccagta atcatgacaa ctgctaaagg tagtgagtat
    14521 gataaagtta aagggttaga tcttggagca gatgattatc tcgtcaaacc ttttgggatg
    14581 atggaaatga tttcacgaat aaaagcagtc ttgagacgta gccgccaagt agattcaaaa
    14641 gctcatatta tcattggaaa tttagagatt gacccgacta attattgggt caaaagggga
    14701 acggaaaaaa tccacttaac cctaaaggaa tttgagttat tagtattatt cttccgtaat
    14761 cccaatagag tctttacaag gcaagaactt ctggataagg tttggggaga acaattttta
    14821 ggagaaacta gaactgtgga tgttcacatt gggacactgc gaacaaaact tggtgaggat
    14881 ggctatttga tcgctacagt aaggggagtt ggataccgtt tggaggaaag acatgactaa
    14941 aaaaattttc cgtacaactt tatccgctag tttgggaatt gttttagtta caattttgat
    15001 gattatggga tttctctata attacttcaa tcgcattcag cgagaacagt tgagaacaca
    15061 aacggcactg gcgtcacaag gaatttcctt tgaaggtaaa gattattttg aaaatcttaa
    15121 aacttcaaat gtgcgtatta cttgggtaga taataaggga caagtgcttt atgataccca
    15181 atcagatgca aaacatatga aaaatcacgc caatcgtcaa gaaattaaag aagctatcaa
    15241 gagtggttat ggtgagagca cgaggtggtc agcgacactt actgagaagt ctatctatgc
    15301 tgcgcaacgt ttaaataatg gaacgatagt tcgtttatca gttgcgcaac aaactatttt
    15361 ttatcttctc cttggtatga tgagtccctt agcaattatc atcttacttg ctattatttt
    15421 atctgtatta attgcacgat atatagcaaa aaaagtatca gagcctttaa acaatattga
    15481 cttagatcat cctttatcaa atgatagtta cgaggagata acaccattat taaggcgtct
    15541 tgatagccat caggctaaga ttcaacatca aaaactgtta ttacaaaagc gtcaaaaaga
    15601 atttgatacc attatttcta aaattaagga aggaatgatt cttttagatg atcaagcaag
    15661 aatcgttagt atcaacgcag aagctttaaa gttattccaa attaatgatg attggcatgg
    15721 gcgctttatg atggaagtta gccgagattt aactctcaaa gacttaattg atcaaggact
    15781 aaaagggaaa aagaaagagg ctaatattga cattgaaaat aatcattacc gcgtcttggt
    15841 aagaccaact acagataaca accgagtaac aggattggtt gttctactgt ttgatgtgac
    15901 tgaccaactc caaatggaac aacttcaacg tgaatttact gccaatgtct ctcatgaatt
    15961 gaaaaccccg cttcatgtta tttctggtta ctcagaactg ttagctaatc aaatggttcc
    16021 aaacgaagaa gtcccacaat ttgcagctaa aattcataaa gaatctgaac gattagttaa
    16081 attggttgag gatattatca acctttctca ccttgatgaa caagaaaaat taccgcaaga
    16141 gacagtaaat ctgtatgact taacacaaaa agttttagaa ggtttacaag ctaaagcaga
    16201 taagaagcac atacaaatca attttaatgg agaagaagct attttgcgag ggaatccagt
    16261 tttattgaat tccttggtgt acaacttgtg tgacaatgct atcacctata atcatgagaa
    16321 aggtcaagta aatgtgactt tgaaaaattc acctgataca attaccttag aagttagtga
    16381 tacgggacta ggcattgcag agaaggacaa aaaacgtatt ttcgaacgtt tctatcgcgt
    16441 cgataaaagt cgctcaaaaa tagttggagg gactggttta ggtttgtcta ttgtaaagag
    16501 tgctttagat tttcacaatg gtagtataaa agtggacagc catttggggc agggaacaac
    16561 aatgaccgtt ttacttcaca aacaataaaa actcacaaat aaaagtctag atgatattat
    16621 ctagactttt ttagtaattc agaaaaaata tataagtaag ggtttacaaa aaacaaataa
    16681 gtgctataat aaaaccataa cgcaaacgtt tgcactatta agtgcataaa aggagattga
    16741 agatgaaaaa taatgtcaaa cagctcttta gttttgaatt ttggcagaag tttgggaagg
    16801 ctttgatggt tgttattgct gttatgccag cagcaggatt gatggttagt attggtaact
    16861 ccattaggct attggatcca tcaaatgttt tattagggcg catagctaat gttattgctc
    16921 aaattggttg gggagttatt ggtaacttac acatcttatt tgcattggct attggtggta
    16981 gctgggctaa agaacgtgcc ggtggtgctt ttgcggctgg cttatccttt attcttatta
    17041 accttattac agggaacttt tttggtgtga aaacagatat gttagcaaac agtaaagcaa
    17101 ctgttcaaac ggtatttggt gcgaccatca gagtttcaga ttactttgtt aacgttcttg
    17161 gacaacctgc tcttaatatg ggtgtatttg tcggtattat ttcaggtttc gttggtgcaa
    17221 ctgcctttaa taaatattat aactatcgta aattaccaga tgctttaact tttttcaatg
    17281 gaaaacgttt tgttccattt gttgttattt atcgttcagt cattgttgca ttaattttat
    17341 cagtattttg gccggttgtt caatcaggta tcaatggctt tggtaagtgg attgcaagtt
    17401 cacaagacag tgctccaata ttagcaccat ttgtctacgg tacgttagag cgtttattgc
    17461 tcccatttgg tttacaccat atgttaacta ttccaatgaa ctacacgcaa ctaggtggca
    17521 catacactgt ccttacaggt gcaactaaag gagcacaggt gttgggacaa gatccactgt
    17581 ggttagcatg ggttggtgat ttaatcaact taaaaggctc aaattcaagt caataccatc
    17641 acttattgac aagtgtcact ccagcacgtt ttaaagtggg gcaaatgatt ggtgcttcag
    17701 gtattttgat gggattatct tatgctatgt atcgtaacgt tgataaagat aaaaaattga
    17761 aatacaagtc aatgtttatt tcagcagcag cggctacttt cttgacaggt gttactgagc
    17821 caattgagta catgttcatg tttgcagcaa tgccactcta ccttgtatat gctgtcgttc
    17881 aaggatgtgc gtttgctatg gcagatattg ttaacctccg cgttcattca tttggtaata
    17941 tagaattttt aacacgtgtt cccatgggaa taaaagcggg ccttggtggt gatattttca
    18001 actttgtttg ggtgacactt ctttttgcag tattgatgta ctttattgca aactttatga
    18061 ttaaaaaatt caatttagca acagcaggac gtaatggtaa ttatgataac gaagaggttg
    18121 ataatgctcc ttcaacagct tctggttctg ctgatgcaaa ttctcaagtt gtacaagtta
    18181 ttaatttact tggtggtcgt gataacattg aagatgtaga tgcatgtatg actcgtcttc
    18241 gtgtcacagt taaagatggg aatagcgtgg gttctgaagc agcttggaaa aaagctggtg
    18301 caatgggatt ggtgttaaaa ggtaatggcg ttcaagctat ttacggacct aaagcagatg
    18361 tccttaaatc agacattcaa gatttgctag attcaggtac agtgattcca atagttgatt
    18421 tagaaacagg ccagcctgta gcagcaaccc aagttacaac ttataaagga ataacagaag
    18481 aaattgtttc ggtggctaat ggtcaagtag aagcattgga tgttgttaaa gaccctgtct
    18541 tttctcaaaa aatgatgggt gatggctttg ctgtggaacc aactgatggc aatatctatg
    18601 ttccagtttc aggtacagtt actagtgtct ttccaacaaa acatgctttt ggtttattaa
    18661 cagaaagtgg cctagaagtt ttagtacata tcggtctaga tacagtagct cttgatggtc
    18721 aaccatttga ggttaaaatt tctagtggtc aaaaagtggt agcaggcgat cttgctgtag
    18781 tagcagattt ggaagctatc aaagcagctg gtaaagagac aagtgtgatt atcgtcttta
    18841 caaatgtttc agatataaaa actgttaaac ttgaaaaatc aggtccacaa atagctaaaa
    18901 cagttgtggc aaaagtcgaa ctgtaacaaa cgataggagc tgggcaagtc tcagcttctt
    18961 tctgatgaaa ggatactcta tggcaaaatg cttaacgtta aatactcatt catggatgga
    19021 agtaaatgct ctaaagaaat tatttgattt agcagagcat atcttccgtg aaaaatatga
    19081 tatcatctgc ctacaagagg tgaaccagag catatcttcc cctcttgcta cgagtagtcc
    19141 aaattatcat ccgattgaag gtacaccagc tcttcatcaa gataactttg ccttgcaatt
    19201 agtgcattac ctaaatcttc aaggtctaca ctatcattgg acatgggctt ataatcatat
    19261 tggttatagt aagtaccatg aaggggtagc gatactttct ctgaaaccat taaagccaga
    19321 tgatatttta gtttcagctg tagacgatga gactgattat catactcgtc gtgctctggt
    19381 agctgagacc actctgaatg acaaagtcgt tacagtagtt agcctccatt tatcatggtt
    19441 tgagaaagga tttgcggagg agtggaaacg actagaaact accctacttg aggtagaaac
    19501 tccactttta ctcatgggtg actttaataa tcctactgga aatcagggtt atgaacttgt
    19561 tcttaatagt ccattagcat taaaggatag ccaccaaata gccaatcatg tatttggaga
    19621 ccatactatt atggctgata ttgatggttg ggaagggaat aaaaaggctc ttaaagttga
    19681 ccatattttt actagcgaag acctttcaat cagttcaagt caagtagttt ttgaaggtgg
    19741 tgaagcacct gtcgttagtg atcattacgg cttggaaatt acgttaaatc tttagattaa
    19801 tctatttaat atattaaaag catcctaaat ttgtctataa aacaaaactt agggtgcttt
    19861 ttgaaaaata tgtcacatta tttaacttct tacgaagttt ggaatgcatg agaaaatatg
    19921 ataaaatgat aataactatt ataggtgatt ttaataaata atttaatcaa ttggtttatc
    19981 tattagaaca attaatacga ggagacgaga gtgaatattt ttattttaga ggataatttt
    20041 attcaacagg cacgtttaga gagaataatt gaggaagtta aaattaggag aagtatacac
    20101 agtaaaatgg taaatacctt tgaaaaacca gttcaattat tggaatcaat ctgtgaaaaa
    20161 ggtatgcata atttatattt cttagacatt gaaataaaaa atgatgagca gaggggctta
    20221 gaggtcgcaa aacaaattag acaaattgat ccttatgctc agattgtttt tgttacaact
    20281 cattcggaat taatgccttt aacctttcgt tatcaggtat cagctttaga ctatatcgat
    20341 aaaggacttt cacaggaagc atttagcaga aggattgaag acgttttact atatgctaat
    20401 aatgcttgca gtaaaaactt actagaagat tctttttatt ttaaatcacg ctatagtcaa
    20461 gtacaagtac cttttaacga tatcctttat atagaaacgt cattaaggtc acatagagtt
    20521 gtcctttata ctgcaaaaga cagaatggaa ttcacagcca ctttggggga tattttaaaa
    20581 caagaaccta ggctctttca atgtcatcgc tcttttttag taaatccctt gaatattttt
    20641 aaagtggata ggtcaaataa actaatctat taaaaaaatg gttcgaattg tttgatttct
    20701 cgtaataaaa taaaagatat tatggtagta atagaggaaa atcaaagagg tagaggaata
    20761 tgaattctac tctatccatc ttagagatag ctttaacgat aatattaaca atatttctct
    20821 tttctaaaat cagtggacgt caattgactt taatagagat tggaatttgt attgctctga
    20881 gattaggact tgtaatttta tttatagcag ctaatactat tattaatctt tcatttctaa
    20941 agtactggct tctccccatc tatatgttaa gtctggcttt cttgttgcta agacctatat
    21001 ctaaaacttt agtagtcttt tatggcttgt ttccaattgt tctcagaaat ctttgttcac
    21061 gttctttagc tttttttgtc tttcctttgt ttaatattga ttttcataca accaaccagg
    21121 ggttttgggg atattttgtt gatgtcttgt ctactctttg tgttctccta tttttaagat
    21181 ggatgcaata cgactttaaa actttgcgaa gtagtgaagt ttcgcccata gataagagaa
    21241 ttctccaatt tacgaatggt tgtatggtca gttattactt actgatgcag ttttcaactt
    21301 atttggaata tgaattagcc atcaatactg ttatttaccg gcaatttttg cttgttgttt
    21361 attggattat atttacggga gcaatagcca gtttagaccg gagacttcga aattctttac
    21421 aggagaggtt agctgttcaa agagaactcc aattggctaa tatgatagat tatagccacc
    21481 atattgaggg gttgtataaa gaagttagga gcttccgaca tgattatagt aacatgttaa
    21541 cgaccttgaa actagggatt gataatgaag atatatctat tgtcaaatca gtttatcagt
    21601 ctatctttaa agaatctaat acacatttcc gacatcctaa gtataatata gaccggttgg
    21661 ttaatgtaga taacccagcc ttaaaaagtc ttttagcagc caagctttca caagcgatag
    21721 acaatcatat tatggtatct atagaagtac cagacagtat taaacctcaa gggatggaac
    21781 tgattgattt tattaccatt gtatctgttt tatgggataa cgctattgag gcatctttag
    21841 agacagacga tcctaagata gccatgtctt attttacatt aaaacataaa caaattttta
    21901 tcatagaaaa ttcaactaag gaaaaggcaa tagatacgtc aacgatattc aattatgaga
    21961 taagtagtaa ggggcggaat agaggaatag gtctttacaa tgtcagggga gttatcaatc
    22021 gttatccaaa tatttctatt gcaacaagta gccaagattt tagtttctgc caaactttcg
    22081 aaatcatatt atagttttaa aaagttcaat ttatctaagt ttattatttt cttaatacca
    22141 tcaatccaac caccaccagt gatattattt agttgtttcg gcgataattc ctgaagctgt
    22201 agttccatct cgattagttt tggctagttc tcgtaagtgg tttaataaat attttgaacc
    22261 atagaacttt gcgattgaag caagtgcagt tactccacaa tccttagtat caacctgacg
    22321 caccaatgct tttttgtacg tcaacattgt gtaattcctt tcttcgttat cgtattaata
    22381 ttttgccata aaaatatata cttattaaaa atagcttaaa cagctattgc aacgcgtcca
    22441 atggtttcta taaggatata aaggtagata gtacttttct gttttgaggc gattcaacga
    22501 catttcaggg aataaacgtt tctattttta tttgaatgct atattaaatg tgtcaagttg
    22561 caacagacaa aaattttata taggagatat tcaaaataaa tgtaaaatca attgaacaat
    22621 tagaagttat ggataatgaa gcattatcac atgttgaagg tggccttggt catagatggg
    22681 tatacagtaa gaaccggtaa acatacttat acaagtgcat ggcatctttc tgaaaatact
    22741 gctgcagcaa atgcaagaaa tcgtatgggc ctttaccctg gaagcgtttg tcgtgtctat
    22801 cttgtataat tgatttttta tttaagcgat agggaatctt ccttgccgct taagtttgta
    22861 tagggagaga caatactatg ttattacagc tagataatat cactaaaagc tacggaaaaa
    22921 aaatagtact taaccagatt tcctaccagt ttactcctgg tttgtatgga ctcttaggag
    22981 caaatggtac tggcaaaaca accttattaa atttgatgag ccactttaca ttcgcagata
    23041 gtgggaatat ttattggaat ggacaggagc agtcggaaga attttatcgt cacattggtt
    23101 ttttaccgca acattttcgc tattatgatc aatttactgg cattgctttc ttaaattata
    23161 tcgctacctt aaaaggtgtt gataaaaaga aagctaaacc agaaattcct agattactag
    23221 agcttgttgg tttaggtgat gtaggtaaaa agaagatttc atcatattcc ggtggtatga
    23281 agcaacgttt aggcattgct caagcactta tcaatgaccc tgaaatactt attctggatg
    23341 aaccaactgt tggacttgat cctaaggaac gggttaaatt tcgccatatt ttgagtcagt
    23401 tatccaccaa taaaattatc attttatcaa cgcatattgt gtcggatgtg gaagctgttg
    23461 ccaaagaaat tatagtatta aaaaatggta aatttatcga acagggcaac accgcacagt
    23521 tgttgaaaac tattgaggga aaagtgtggg aaataacgac tgaaccagat ttatctcaga
    23581 tacccaatat tgctattgtt aatgaaaaag tcatttcaga tagtctggta tttcgtgtag
    23641 tgagtgatat ttgtccgagt gattcggcac agttggttgt tccgactcta gaagatttct
    23701 acatctatca ttttagaggg gagtaagtaa gatgatatca atttttcctt ttgaattaaa
    23761 aaaacttcta caacgtaagg cagcattggg ggcttttatt atcttccttc ttgctatttt
    23821 tggtctattt taccagcatt tttttgttgg gcaaattagt ggctattctg cagataaaat
    23881 acatggtcga gaagcggttg caattaatcg tagaatagct gaaaaatatt caggggattt
    23941 atctgaagaa cgagtaacaa atattattaa aaactatgtt aatccgccag caggacgccc
    24001 ccctatattt gatgttttta gttattatgt ggcagacact tttgatcgtg attttttaat
    24061 ttactataac caattgaaag atgataaaac aattgatttt aataaaataa atattaaaga
    24121 tcaaaaacag ttgggttctg cgctaccact aaaacaacta aagctaggta actttgctcc
    24181 ttggaatcaa ctttttgaag taagtaatag tacttatatt ttaattgtct tatttactct
    24241 attcttatca gctcctcttt tttcaggaga tagggctaag aagattaatc ctatactact
    24301 gacgactcgg tatggacgtg gtcagttgac ctgggctaag ttagctagta caatgaccat
    24361 cggtatttcc acatttatcc tagcatatgc cctcattctg acggtatttg cagattattt
    24421 tggttttagt ggttgggaga caagtgttca actgaattta tactggacat cacctttggc
    24481 taatattatg ggatttcctg tacctatgac aatatcaact gcttttgttc atattattgt
    24541 cttacaattt gttggactcc tttttatagc cagtcttaat ttctttatat taagtatcac
    24601 aaattcgcca ttaacatctt ttgctgtttc aacgcttgcc ttttttgctc ctagcttttt
    24661 aatggctatg tttaataaag gtgtgataaa caagttatta actattttct cagtatcaac
    24721 gacagacact tcagctttac ttttaaaatt atcacgtaat ggtagtcatg gctttttctt
    24781 tgatagtttt tctgctaatg aaatctgtct gattagtcta cgtatgttaa ttgcagtgtt
    24841 attgatagct tatacctatc gtttaatgcg acgaagaagt ttatagttcc cactggttat
    24901 ctgaacaaga aattattcta agctgatttt cttttgcgaa atcagctttt ttatttccta
    24961 atatggttga taaggtatta acaataatta tcagatacca tttaaggcgt ttcaatgact
    25021 tttggggata attgagatat tcttctgaag agattggtat catagtaatg ataacttata
    25081 gaaagtaatg ttaaggatga agagaagtca gtgcatggat aataagcagc ataatacatc
    25141 aatgattagc ttgctacaat atcttttttc catactggtc attttagttc atagtggaag
    25201 gttatttagt caggatgtca ttcattttac ctttaaaagt ttcctaggtc ggatggctgt
    25261 tccttatttt ttgatttgta ctgctttctt tttgagaggt cgtatacagc aaggtttgtg
    25321 taatcactcc tactttcgta aactgattaa aaagtatagc atgtggacta tcatttattt
    25381 gccttatggt tatttctttt ttgaatctct aaatatagcg aagatttatt tgctgccggg
    25441 ttttattgtg gcatttttgt atttaggtat gtcacatact ctttggtaca tccctgctgt
    25501 tattctaggt tggttcatta ttcaaggatt attgaagtat gtaggaacta gggggacatt
    25561 tatcacagtt gttgtactct attgtattgg agcagtagag acctattctg tctttgtaca
    25621 gtcaacaaaa ttttatcccc tgatgtccac ttatatgtca atttttcaga caacacgaaa
    25681 cggattattt tacactccag tttatcttct agcaggatat ttactctatg attattttaa
    25741 tactgaccta tttaccaaat cgcgtggtct taagtatatt ctcttccttt tacttctagc
    25801 tttagagaat gttcttattt attttaatca agggttggat aaaaatttct tcttattagc
    25861 tcctttatgt gcagtattct tatttaactg gagtatacgt acatcactgt ttaagggcta
    25921 tcgtttaagt cctttaaaac agttgagtgt ctattatttc ttccttcacc ctctttttgt
    25981 tggcatagtg tcttactgtt taaaatcaac ttccttagtt gcacaccatc aaggaaaagt
    26041 gatttttgtg gtgaccttgg cattaacaca tgcttgtgct ctactcataa taaaggttgt
    26101 taataagata ccctatcaaa aaattattcc attatctctt tcgaaatagt gattcatata
    26161 agagaaagtt taaagttata gtgatgtaaa ggttgtgtaa actattagga tacatgacct
    26221 ttttatt
//
LOCUS       DATRQA010000009        26178 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.009, whole genome shotgun sequence.
ACCESSION   DATRQA010000009 DATRQA010000000
VERSION     DATRQA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26178
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.009"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..153
                     /locus_tag="VBD27_000294"
     CDS             <1..153
                     /locus_tag="VBD27_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEO7024332.1"
                     /translation="DWSKMIKMSPQNISKLKVFILLISIVGGYVVSHFLLEVIQLCQS
                     IFWALQ"
     gene            216..1487
                     /gene="murA"
                     /locus_tag="VBD27_000295"
     CDS             216..1487
                     /gene="murA"
                     /locus_tag="VBD27_000295"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO7024333.1"
                     /translation="MDKIIVEGGQTRLQGQVVIEGAKNAVLPLLAATILPSQGKTLLT
                     NVPILSDVFTMNNVVRGLDIQVDFNCDKKEILVDASGDILDVAPYEFVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPIDLHLKGLEAMGATITQNGGDITAQAEKLKGANI
                     YMDFPSVGATQNLMMAATLASGTTTIENAAREPEIVDLAQLLNKMGAKVKGAGTETLT
                     IIGVDALHGTEHDVVQDRIEAGTFMVAAAMTSGNVLVKDAIWEHNRPLISKLMEMGVE
                     VSEEEDGIRVKADTKKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMIETVFEN
                     RFQHLEEMRRMGLQTEILRDTAMIHGGRALQGAPVMSTDLRASAALILAGMVAQGQTV
                     VGQLTHLDRGYYQFHEKLAALGANIKRVSEA"
     gene            1489..1680
                     /locus_tag="VBD27_000296"
     CDS             1489..1680
                     /locus_tag="VBD27_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO7024334.1"
                     /translation="MASSGWKYVLKQIGLIVLVILLALLFLAVGLMLGYSVFGDGEHA
                     YSILSLDKWQNIIGKFLGK"
     gene            1755..2612
                     /locus_tag="VBD27_000297"
     CDS             1755..2612
                     /locus_tag="VBD27_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7024335.1"
                     /translation="MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHILKQVTDLII
                     SNESGKNTSLKNISGTPSRELSESVLTSNVKKQLGTNIAWNQSGAFIINQNKTDLNAK
                     VSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
                     KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYET
                     LVRKALDRHKTVRYRVTLIYDRDNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDY
                     ATGKVKQTK"
     gene            2903..3943
                     /gene="pheS"
                     /locus_tag="VBD27_000298"
     CDS             2903..3943
                     /gene="pheS"
                     /locus_tag="VBD27_000298"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO7024336.1"
                     /translation="MDLQKQLEELKISTQEKLKEMTGNHTKELQDLRVQVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDILTKAFEEQAKVVEAAKIQAQLESESVDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQVVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTMDQHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLQLISQKMFGAERKIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKQTGWIEILGAGMVHPSVLEMSGIDSEKYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDVRFTDQF"
     gene            4026..4546
                     /locus_tag="VBD27_000299"
                     /pseudo
     CDS             4026..4546
                     /locus_tag="VBD27_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            4600..7005
                     /locus_tag="VBD27_000300"
     CDS             4600..7005
                     /locus_tag="VBD27_000300"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO7024337.1"
                     /translation="MLVSYKWLKELVDVDVTTAELAEKMSTTGIEVEGVETPAEGLSK
                     LVVGHIVSCEDVPDTHLHLCQVDTGGDELRQVVCGAPNVKTGINVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSESIIPKEFSDGIQILPEGAIPGDSIFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYGKKVHFEEKNLIEEAERAADKISVVIESDK
                     VLSYSARIVKNVTVAPSPQWLQNKLMNAGIRPINNVVDVTNYVLLTYGQPMHAFDFDK
                     FDGTTIVARNAENGEKLITLDGEERDLIADDLVIAVNDQPVALAGVMGGQSTEIDSSS
                     KTVVLEAAVFNGTSIRKTSGRLNLRSESSSRFEKGINYDTVSEAMDFAAAMLQELAGS
                     QVLSGQVTEGVLPTEPVEVSTTLGYVNTRLGTELTYTDIEEVFEKLGFAISGSEVKFT
                     VLVPRRRWDIAIQADLVEEIARIYGYEKLPTTLPEAGATAGELTSMQRLRRRVRTVAE
                     GVGLSEIITYALTTPEKAVQFSTQATNITELMWPMTVDRSALRQNVVSGMLDTIAYNV
                     ARKNSNLAVYEIGKVFEQTGNPKEDLPTEVETFTFALTGLVEEKDFQTKAKPVDFFYA
                     KGIVEALFIKLKLDVTFVAQKGLASMHPGRTATILLDGKEIGFVGQVHPQTAKQYDIP
                     ETYVAEINLSTIESQMNQALIFEDITKYPSVSRDIALLLAESVSHHDIVLAIESSGVK
                     RLTAIKLFDVYAGNNIAEGYKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLVEKVNA
                     EIR"
     gene            complement(7074..7976)
                     /locus_tag="VBD27_000301"
     CDS             complement(7074..7976)
                     /locus_tag="VBD27_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEO7024338.1"
                     /translation="MKIDDLRKSDNVEDRRSSSGGSFSSGGSGLPILQLLLMRGSWKT
                     KLVVLIILLLLGGGGLTSIFNDSSSPSSYQSQNVSRSVDNSATREQIDFVNKVLGSTE
                     DFWSQEFQTQGFGNYKEPKLVLYTNSIQTGCGIGESASEPFYCSADKKIYLDISFYNE
                     LSHKYGATGDFAMAYVIAHEVGHHIQTELGIMDKYNRMRHGLTKKEANALNVRLELQA
                     DYYAGVWAHYIRGKNLLEQGDFEEAMNAAHAVGDDTLQKETYGKLVPDSFTHGTAQQR
                     QRWFNKGFQYGDIQHGDTFSAEHL"
     gene            8154..11387
                     /gene="rexB"
                     /locus_tag="VBD27_000302"
     CDS             8154..11387
                     /gene="rexB"
                     /locus_tag="VBD27_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEO7024339.1"
                     /translation="MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKER
                     AVLETLPQEASFAITITRFAQLARYFTLNQPNQKESLNDIGLAMIFYRALASFEDGQL
                     KVFGRLKQDASFISQLVDLYKELQTANLSILDLKYLHSPEKFEDLLAIFLVVSDLLRE
                     GEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIII
                     GAYASQKAYKTNFTNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIE
                     GKHDFTNSSHILDDTAKDCITIWECINQKDEVEHVARAIRQKLYQGYRYKDILVLLGD
                     VDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAEDVLNLFKT
                     GIYGEIGQDDLDYFEAYISYADIKGSKKFFTDFVVGAKKFDLGRLNTIRQSLLTPLES
                     FVKTKKQDGIKTLNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQF
                     QTIFGQEKLNLDEFLSLLNSGMMQAEYRMVPATVDVVTVKSYDLVEPHSNQFVYALGM
                     TQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTRENLKKNHFAALSLFNAAKQA
                     LVLTIPQLLNESEDQMSPYLVELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDL
                     YRSAIDKEMTKEEQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPE
                     DHPLKLSSSALTTFYNNQYKYFLQYVLGLEEQDSIHPDMRHHGTYLHRVFEILMKNQG
                     IESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATILRQDSQMTVE
                     SEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVT
                     YIDAISRDKEVEQKPPIFGAMYLHMQEPRQDLSKIKNLDDLVTKNHQALTYKGLFSEA
                     EKEFLANGKYHLKDSLYSEAEIAILQAHNQSLYKKASETIKSGKFLINPYTEDAKTVD
                     GDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL"
     gene            11377..15000
                     /gene="addA"
                     /locus_tag="VBD27_000303"
     CDS             11377..15000
                     /gene="addA"
                     /locus_tag="VBD27_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEO7024340.1"
                     /translation="MTFKPFLNPEDIAVIQTEEKNSDKKQKRTPEQIEAIYTFGNNVL
                     VSASAGSGKTFVMVERILDKLLRGVPIESLFISTFTVKAAGELKERLEKKINESLKSA
                     ESDDLKQFLTQQLVGIQTADIGTMDAFTQKIVNQYGYTLGISPIFRILQDKNEQDVIK
                     NEVYADLFSDYMTGKNAASFIKLVKNFSGNRKDSKAFREMVYKVYAFSQSIDNPKRWM
                     QTVFLKGAQTYTDFEAIPDQEVSSLLNVMQTTANQLRDLTDQEDYKQLTAKGVPTANY
                     KKHLKIIENLVHWSQDFNLLYGKKGLANLARDITNVIPSGNDVTVAGVKYPIFKQLHN
                     RIVGLKHLEVIFKYQGESLFLLELLQSFVLDFSEQYLQEKIQENAFEFSDIAHFAIQI
                     LEENHDIRQIYQDKYHEVMVDEYQDNNHTQERILELLSNGHNRFMVGDIKQSIYRFRQ
                     ADPQIFNDKYKAYQDNPSQGKLIILKENFRSQSEVLDSTNSVFTHLMDEEVGDILYDE
                     SHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLH
                     KEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRS
                     IDNPLNDYALVALLRSPMFGFNEDDLTRIAIHDVKMAFYHKVKLSYHKEGHHSDLITP
                     ELSSKIDYFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYAL
                     ALRANQFEKTGFKGLSRFIRMIDKVLENENDLADVEVALPQNAVNLMTIHKSKGLEFK
                     YVFILNIDKKFSMVDITSPLILSRNQGIGIKYVADMRHELEEEILPAVKVSMETLPYQ
                     LNKRELRLATLSEQMRLLYVAMTRAEKKLYLVGKASQTKWADHYDLVSENNYLPLASR
                     ETFVTFQDWLLAVHETYKKQELFYDINFVSLEELTDHHIGMVNPSLPFNPDNKAENRQ
                     SEDIVHAISVLESVEQINQTYKAAIELPTVRTPSQVKKFYEPILDIEGVDVMETITKT
                     SVDFKLPDFSTSKKQDPAALGSAVHELMQRIEMSSHVKMEDIQKALTEVNAETSIKAA
                     IQIEKINYFFQENSLGKYIQEEVEHLHREAPFAMLKEDPESGEKFVVRGIIDGYLLLE
                     NRIILFDYKTDKFVNPLELKERYQGQMALYAEALKKSYEIERIDKYLILLGGKQLEVV
                     KMD"
     gene            15013..15930
                     /locus_tag="VBD27_000304"
     CDS             15013..15930
                     /locus_tag="VBD27_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7024341.1"
                     /translation="MIVEQKFGNGFTWINIEAEQLRTETSEIQAKYLDSEIITYALDD
                     YERAFMECSHIKGKEVLTIIFNTIDLKQKESYYETVPMTFCLSHDRLITVTRSRNSYM
                     LELLQKYLDRNPDVSPKKFLFAALTLITKQYFNVVSKIDREKDILNRQLREQTTNKRL
                     LAMSDLETGSVYLLTAANQNALVLEQLDVHPSQRFNSEVEKEQLSDALIEAHQLVSMT
                     QLNSQVLSQLSSTFNNVLNNNLNENLTGLNIISINLAIIAAITGFFGMNIPLPLTESR
                     SSWLIVIATSVLLWVIIAQILKRVFNLRK"
     gene            complement(15962..17338)
                     /gene="mnmE"
                     /locus_tag="VBD27_000305"
     CDS             complement(15962..17338)
                     /gene="mnmE"
                     /locus_tag="VBD27_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO7024342.1"
                     /translation="MSITKEFDTIAAISTPLGEGAIGIVRISGTDALKIASKIYRGKD
                     LSAIQSHTLNYGHIVDPDKNEILDEVMLGVMLAPKTFTREDVIEINTHGGIAVTNEIL
                     QLILRHGARMAEPGEFTKRAFLNGRVDLTQAEAVMDLIRAKTDKAMDIAVKQLDGSLK
                     TLINNTRQEILNTLAQVEVNIDYPEYDDVEEMTTTLMREKTQEFQALMENLLRTARRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREEKAIVTDIEGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRDTDDIVEKIGVERSKKALEEADLVLLVLNSSEPLTLQDRSLLELSKESNRI
                     VLLNKTDLPQKIEVNELPENVIPISVLENENIDKIEERINDIFFDNAGMVEHDATYLS
                     NARHISLIEKAVDSLKAVNEGLELGMPVDLLQVDMTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     gene            17466..19376
                     /locus_tag="VBD27_000306"
     CDS             17466..19376
                     /locus_tag="VBD27_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7024343.1"
                     /translation="MIILQGNKIERSFSGDVLFDNINIQVDQRDRIALVGRNGAGKST
                     LLKILVGEEAPTKGEINKKRDLSLSYLAQDSRFQSENTIFQEMLQVFDSLREVEKRLR
                     ELELQMGQVSGSDLEQLMKTYDILSEEFREKGGFTYESDIKAILNGFKFNSDMWEMPI
                     SELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIDTIAWLENYLVNYQGALIIVSHD
                     RYFLDKVATVTYDLTTHSLDRYVGNYSKFMDLKAEKIATEEKNFEKQQKEIAKLEDFV
                     QRNIVRASTTKRAQARRKQLEKMERLDKPNVEQKSANMTFHAGKVSGNVVLTLENAAI
                     GYEGVSLSEPIDLDVKKFDAIAIVGPNGIGKSTLIKSLVGQIPFIKGEAKLGANVETG
                     YYDQSQSNLTKTNTVLDELWNAFSTTPEVEIRNRLGAFLFSGDDVKKSVSMLSGGERA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIEFDGTLLFVSHDRYFINRVAT
                     KVLEISDKGSTLYLGDYDYYLTKKAELEELARLNEEEVSASKTEIDVTSDYETQKANQ
                     KEFRKITRRVAEIEARLEVLENDENNINGLMLETNDIGKLSDLQKELESIQEEQLLLM
                     EEWENLNMRLDL"
     gene            19526..20494
                     /locus_tag="VBD27_000307"
     CDS             19526..20494
                     /locus_tag="VBD27_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEO7024344.1"
                     /translation="MVTLSKEQHLDMFLKMQRIRDVDMKFNKLVRRGFIQGMTHFSVG
                     EEAASVGAIQDLTDSDIIFSNHRGHGQTIAKGIDIGGMFAELAGKATGTSKGRGGSMH
                     LANLEKGNYGTNGIVGGGYALAVGAALTQQYEGTDNIVIAFSGDSATNEGSFHESVNL
                     AAVWNLPVIFFIINNRYGISTDITYSTKIPHLYMRADAYGIPGHYVEDGNDLMAVYEK
                     MHEVINYVRSGNGPAIVEVESYRWFGHSTADAGVYRTKEEVDSWKAKDPVKRYRAYLI
                     ENEIATEEELAAIEAQVIKEVEEGVKFAEESPFPDMSVAFEDVFVD"
     gene            20569..21567
                     /locus_tag="VBD27_000308"
     CDS             20569..21567
                     /locus_tag="VBD27_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO7024345.1"
                     /translation="MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTS
                     VGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTGLRPIVDLTFMDFVTIAMDAIVNQGA
                     KTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTVNESKAL
                     LKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVMQAAEEVAEEGINVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEI
                     ATMVAESEAFDYLDHPIVRLASEDVPVPYSRVLEQGILPDVAKIKDAIYKVVNKGK"
     gene            21694..23082
                     /locus_tag="VBD27_000309"
     CDS             21694..23082
                     /locus_tag="VBD27_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEO7024346.1"
                     /translation="MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKT
                     NMEIEAEDSGVLLKITHGNGDVVPVTETIGCIGAEGEEVTEASSSENTSVEENATQVT
                     SEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRVHREDVE
                     SFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVKTKA
                     TPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLI
                     DPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGL
                     DDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDL
                     KNLMENPFGLLI"
     gene            23142..24899
                     /gene="lpdA"
                     /locus_tag="VBD27_000310"
     CDS             23142..24899
                     /gene="lpdA"
                     /locus_tag="VBD27_000310"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO7024347.1"
                     /translation="MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKT
                     NMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIGEEGEEVGTSSPSADATITAEDGQSV
                     SGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAIVEKTEF
                     GGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTL
                     TGGVRGLLKANKVEIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIES
                     PLVLTSDDILDLREIPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKE
                     VSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSIGRVPQMN
                     GLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALG
                     GNKRKAHLDFTPAAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAH
                     GFVKVIAEPKYKEILGVHIIGPAAAELINEASTIMENELTVYDVAQSIHGHPTFSEVM
                     YEAFLDVLGEAIHNPPKRK"
     gene            24997..25986
                     /locus_tag="VBD27_000311"
     CDS             24997..25986
                     /locus_tag="VBD27_000311"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO7024348.1"
                     /translation="MKYIVNTSNDPAYNVALEAYAFQKLTDIDEIFILWINEPAIIIG
                     RHQNTIQEINKEFIDKNGIHVVRRLSGGGAVYHDLNNLNYTIISNNTQEGAFDFQTFS
                     KPVIDTLAKLGVKAEFTGRNDLEINGQKFAGNAQAYYKGRMMHHGCLLFDVDMSVLGQ
                     ALKVSKDKIESKGIKSVRARVTNIVDHLSDKITVQEFSDAILAQVKEEYPEMDEYVLS
                     DAELSEIQAMRDNQFATWDWTYGKAPEYTIERGVRYPAGKITTYANVENSTIKSVKIF
                     GDFFGVKPVDDIEKMLEGVRYDYKDVLAALKTVDTSQYFSRMTPEEITKAIVD"
BASE COUNT         8635 a         4190 c         5278 g         8075 t
ORIGIN      
        1 gactggtcta aaatgattaa aatgtcccct caaaacatct caaaattaaa agtttttatt
       61 ttgttgataa gtatagttgg tggctatgtg gttagtcatt ttttattgga agtgattcag
      121 ttgtgtcaat caatcttttg ggctctccaa taaaggaaat attagctgaa ctatgatata
      181 atcaacttac tattgttata agaatggaga ttcaaatgga taaaattatt gtggaaggtg
      241 ggcaaacgcg gcttcaaggt caagttgtta ttgaaggagc taaaaatgca gttttacctc
      301 ttttagcagc cacaatttta ccaagccaag ggaaaacatt attgaccaat gttcctatct
      361 tgtctgatgt atttacaatg aacaatgttg ttcgtggttt agatattcaa gttgacttta
      421 attgcgataa aaaagaaatc ttagtggatg cctcaggtga tattttagat gttgcacctt
      481 atgaatttgt tagtcaaatg agagcttcta ttgttgtttt gggacctatt ttagctagaa
      541 atggccatgc taaggtttca atgccaggcg gttgtacaat tggtagccga ccaattgacc
      601 ttcatcttaa aggtttagaa gctatgggag ctacgattac acaaaatggt ggtgatatta
      661 ctgcccaagc tgaaaagctt aagggggcta atatatacat ggattttccc tcagtcggtg
      721 ccactcaaaa cttgatgatg gctgctacac ttgcttcagg cacaacaacg atagagaatg
      781 ctgcacgtga accagaaatt gtagaccttg cgcagttact aaataaaatg ggagctaaag
      841 tcaagggtgc tggtacggaa acattaacga tcattggtgt tgatgcttta catggtacag
      901 aacatgatgt tgtccaagat cgtattgaag ctggaacctt tatggtggca gctgctatga
      961 cttctggaaa tgttctagta aaggatgcta tttgggaaca taaccgtcct ttaatctcta
     1021 aattgatgga gatgggtgtt gaagtgtcag aggaagaaga tggtattcgt gtaaaagctg
     1081 atactaaaaa attgaagcca gtgactgtga aaacactccc tcatccagga tttcctactg
     1141 atatgcaagc tcaatttaca gctctaatgg cagtagttaa tggcgaatca actatgattg
     1201 agacagtttt tgaaaatcgt tttcaacatt tggaagaaat gcgtcgcatg ggattacaaa
     1261 cggaaatttt acgagatact gcaatgattc atggtgggag agccttacaa ggtgcaccgg
     1321 tcatgtctac tgacttacga gctagtgcag ctttaatctt ggcaggaatg gttgcgcaag
     1381 ggcaaacagt agttggccaa ttaacccatt tagaccgtgg ttattatcaa ttccatgaaa
     1441 aattagcggc tcttggtgct aatattaaac gtgtaagtga ggcgtaatat ggcatctagt
     1501 ggttggaaat atgtactaaa acaaatcggt ctaatagtat tagtcatact gttagccttg
     1561 ctgtttttag cggttggatt gatgttgggt tatagtgttt ttggtgacgg agagcatgct
     1621 tactctattt tatcgctgga taaatggcaa aatattattg gaaaattttt aggaaaatag
     1681 aaagagccac tataagttat ttgatgctta tagtggtttc ctttgagagt taattttagc
     1741 agtagaataa tacaatgaca aaaaaacaaa aacaaataac agcttcgatt gtaactttgc
     1801 ttttttcatt actggcaata gggttaggtg aaagtaaatc tttgcctaat gatcacattt
     1861 taaaacaggt gacagattta attattagta atgaatctgg caaaaatact tcccttaaaa
     1921 atatatcagg aacccctagt agagagttat ctgaatctgt tttgactagc aatgttaaaa
     1981 aacaattggg aacaaatata gcttggaacc agtcaggtgc ttttatcatt aatcaaaata
     2041 aaacagattt gaatgctaaa gtgtcaagtg caccatatgc tattaatgaa ataaagaagg
     2101 ttaataacca aattgttcca actaaagcaa atgccttatt aacaaaagca actcgtcaat
     2161 atcgaaatcg agaagaaaca gggaatggta gaacttattg gaaaccagct ggatggcatc
     2221 agataaatgg attaaaaggg agctataacc acgctgtgga cagaggccat ttaattggtt
     2281 atgctttagt agggagttta agaggatttg atgcctccac aagtaatcct aaaaatattg
     2341 ccacacaagc agcttgggca aaccaagcta atagcaacca atcaacaggg caaaattatt
     2401 atgaaacttt agttcgtaaa gcattggatc gtcataaaac tgttaggtat cgtgtgactt
     2461 taatttatga cagagataat cttttatctt cgggatcaca tattgaggct aaatcttcag
     2521 atggaagttt agaatttaat gtttttattc ctaatgtaca atcgggatta ttatttgatt
     2581 atgcaacagg aaaagtgaag cagacaaagt aatctttatc taatagtgct tgttaaagac
     2641 tgaaaaattt gataaaatag tccttaattg aatagaggtc tgtgagacta gtaaaccaag
     2701 ggaagctgct tagggagttc agccgtgact gcaagctgaa tcagtgaaag agggtggaat
     2761 tcactcacaa ataggactat aattaaggtc tgattggtcg atcagataga aaacggatgg
     2821 taccgcgtgt caacgctccg attaaggggg agtggcacgc tttttttgtt ttcaaagcta
     2881 cttattttga cttggaggaa agatggattt acaaaaacaa ttagaagagt tgaaaatatc
     2941 aactcaagaa aagcttaaag aaatgactgg gaaccacact aaagaattac aagacttacg
     3001 tgtacaagtt ttaggaaaaa aaggttctct aacagaatta ctaaaaggtc tgaaagattt
     3061 atctaatgat ttacgaccag ttgttggaaa acaagtcaat gaagtacgtg atattttaac
     3121 aaaagcgttt gaggaacaag ctaaagtagt ggaagctgcg aagattcaag cacagttaga
     3181 gtctgagagt gtcgacgtta ccttgcctgg tcgtcaaatg acattaggtc accgtcatgt
     3241 cttaacacaa actagcgaag agattgaaga tatcttttta ggtatgggat tccaagtggt
     3301 tgatggtttt gaagttgaga aagactacta taattttgaa cgaatgaatt taccaaaaga
     3361 tcatccagcc cgtgatatgc aagatacttt ttatattaca gaagaaatct tgctacgtac
     3421 tcatacttcg cctgttcaag cacgaacaat ggaccaacat gatttttcta aagggccttt
     3481 aaaaatgatt tctcctggac gtgttttccg tcgtgatact gatgatgcta ctcatagcca
     3541 tcaattccat caaattgaag gcttagttgt tggagaaaat atttcaatgg gcgatctcaa
     3601 aggaacactt caactgattt ctcaaaaaat gtttggagca gagcgtaaaa tccgacttcg
     3661 tccttcatat ttcccattca cagaaccttc agttgaagtt gatgtctctt gcttcaagtg
     3721 cggaggtaag ggatgtaatg tctgcaaaca gacaggttgg attgagatat tgggtgctgg
     3781 tatggttcac ccaagtgttt tggaaatgtc tggtattgat tctgagaaat actccggttt
     3841 tgcctttggc ttaggtcaag aacgtattgc aatgttacgc tacggtatca atgacattcg
     3901 tggcttctat caaggtgatg ttcgcttcac agatcaattt taagatttta gatagcaagt
     3961 agcatcattg gtgtgatgat aaatttatgt taacgctatt accttactca ataggaggtt
     4021 ttctaatgtc tcaaattact tttaaaaagg ttggccttga taatgtgaat attttacaga
     4081 atcttgctat agaaaccttt cggcagacgt tttctcatga taatagtgag gaacaacttc
     4141 aagcattttt caatgagtct tacactttac cagtattaaa atcggaaatt acccatgctg
     4201 aatctgatac ttattttgtt tatcttgata cagatttagt gggttattta aaagtaaact
     4261 ggggaagtca gcaaactgaa aaaaacttga taaagcattt gaaattcaaa ggatttatct
     4321 tctagatgcc taccaaggga aaggtatagg taaggctact ttcgaatttg ctttggattt
     4381 agcttataaa tcaggcttag attgggcttg gttaggtgtt tgggagttta atcataaagc
     4441 tcaggcattt tacgctaaat acggatttga aaaattttct gagcaccaat tttcagtagg
     4501 tgataaagtg gatacggatt ggttattaag aaaaagttta cactaaaaac tacaaagcac
     4561 ttgtataaaa ataaaagaaa ataaatagag agaaattaca tgttagtatc gtataaatgg
     4621 ttaaaagagt tagtggatgt tgatgtcaca acagcagaac tagctgagaa aatgtcaacc
     4681 actggtattg aggttgaagg tgtggagaca ccagcggaag gtttatcaaa attagttgta
     4741 ggtcatattg tttcgtgtga agatgttcca gatacccact tacatttgtg tcaagttgat
     4801 acagggggcg atgaacttcg tcaagttgtc tgtggtgctc caaatgtcaa gactggtatt
     4861 aatgttatcg tagcggttcc tggtgctcgt attgcagata actataagat taaaaaaggt
     4921 aaaatccgtg gtatggagtc acttggaatg atttgttcac ttcaagagtt gggcttatcg
     4981 gaaagtatca ttccaaaaga gttttctgat ggtatccaaa ttttacctga gggagctata
     5041 cctggtgata gtattttctc ttatcttgac ttagatgatg aaattattga attatcaatt
     5101 acaccaaacc gtgcagatgc tctttcaatg cgtggtgttg cccatgaggt agcagcaatt
     5161 tatgggaaaa aagttcattt tgaagaaaaa aatctcattg aagaagcaga aagagcagct
     5221 gataagattt ctgttgtcat tgaatcagat aaagttttat cttactcggc tcgtattgtt
     5281 aagaatgtaa cagttgcacc aagtccacaa tggcttcaaa ataaactgat gaatgctggt
     5341 atacgtccaa ttaataatgt tgttgatgtg acaaactatg ttcttttgac ttatggtcaa
     5401 ccaatgcatg cttttgattt tgataaattt gatgggacaa ctatagtagc acgtaatgct
     5461 gagaatggtg aaaagttaat tactcttgat ggtgaagagc gtgacttaat agctgatgac
     5521 ttggtcattg cggtaaatga tcaaccagtt gctttggcag gcgttatggg aggacaatca
     5581 actgaaattg atagttcttc taagacagtg gtccttgaag cggcagtatt taatggaaca
     5641 agtatccgca aaacaagtgg aagactcaac cttcgttcag agtcatcttc tcgctttgaa
     5701 aaaggtatca attatgatac ggtttcagag gctatggatt ttgcggctgc aatgttacaa
     5761 gaattggcag gcagtcaagt tctttctggg caagttacag aaggagttct tccaactgag
     5821 ccggtagagg tatcaacgac tcttggttac gttaatactc gtttgggaac agaacttacc
     5881 tatactgata ttgaagaagt atttgaaaaa ctcggctttg ctatttctgg ttcagaagtg
     5941 aaatttactg tgttggtacc gcgtcgtaga tgggatattg ccattcaagc tgacttagtt
     6001 gaagagattg cccgtattta tggttacgaa aaattaccaa caacattgcc agaagccgga
     6061 gcgactgcag gagaattaac gagtatgcaa cgcttacgtc gtagagtccg tacggttgct
     6121 gaaggagtag gattatctga aattatcacc tatgctttaa cgactccaga aaaagcagtg
     6181 cagttctcaa ctcaagcaac taatataact gaacttatgt ggccgatgac tgttgatcgc
     6241 tcagcacttc gccaaaatgt tgtttcagga atgcttgata caattgctta taacgttgct
     6301 cgcaaaaaca gtaaccttgc agtttatgag ataggaaaag tttttgaaca aactggtaat
     6361 ccaaaagaag atttaccaac tgaagtagag acgtttactt ttgccttaac aggtctagta
     6421 gaggaaaaag atttccaaac aaaagccaag ccagttgatt tcttctacgc caaaggtatt
     6481 gtagaggcgc tctttattaa attaaaatta gatgttactt ttgtagcaca aaaaggttta
     6541 gcaagtatgc acccaggtcg tacagcaact atcttattag atgggaaaga gattggtttt
     6601 gttggtcaag tacacccaca aactgcaaaa caatatgata ttcctgaaac ttatgttgct
     6661 gaaatcaatc tatcaactat tgaaagtcaa atgaaccaag cgttgatttt tgaagatatt
     6721 acgaagtatc cgtctgtatc tcgcgatatt gcattattac ttgcagaatc tgttagtcat
     6781 catgatattg tgttagcaat tgagtcatca ggggtaaaac gtctaacagc gattaaactg
     6841 tttgatgtct acgctggaaa taatattgca gaagggtata aatcaatggc ttatagctta
     6901 actttccaaa accctaatga caatctaacg gacgaagaag tggctaaata tatggaaaaa
     6961 atcactaaat cactcgttga gaaagttaat gcagaaataa gataatttat atcttacgtt
     7021 gaaaaataaa taaaaaacag catcaattaa gatgctgttt ttttatttaa aatttataga
     7081 tgttctgcgg agaaagtatc accgtgttgg atgtcaccat attgaaagcc tttgttaaac
     7141 cagcgttggc gttgttgagc tgttccatgg gtaaagctat caggcactaa ttttccgtag
     7201 gtttctttct gaagggtatc gtctccgacg gcgtgggcag cattcatggc ctcttcaaag
     7261 tctccttgtt ctaagagatt ttttcccctg atgtagtgag cccatacccc tgcataataa
     7321 tctgcttgaa gttctagccg aacatttaaa gcatttgctt ctttcttagt aagtccgtgt
     7381 cgcattctat tatacttatc cataatgcct aactctgttt gaatgtggtg accaacttca
     7441 tgggcgatga cgtaggccat agcaaaatca ccagtagcac catatttatg tgataattca
     7501 ttgtaaaaag aaatatcaag atagattttt ttatctgctg aacaataaaa tggttctgaa
     7561 gcagattcac ctataccaca acctgtttga atcgaattgg tgtaaagaac aagttttggt
     7621 tccttataat ttccaaaacc ttgggtttgg aattcttgtg accagaaatc ctcagttgat
     7681 ccaaggactt tattaacgaa atcgatttgt tctctcgttg cgctattatc aacagaacgt
     7741 gagacattct gagattggta actagaaggt gaggatgagt cattaaaaat gctggttagt
     7801 ccccctccgc caagtagcag taagatgatt aaaaccacaa gcttggtttt ccaactccct
     7861 cgcatcaata aaagttgaag aatcggtaat ccacttcctc cgctagagaa tgaacctccg
     7921 ctactggagc gacgatcttc aacattgtcg ctttttctta ggtcatctat tttcataagt
     7981 atctccaaag tgttttatag gctcattata aactatttta ataagaaatg cttgaatttt
     8041 taactaaata tcttccccta gatttgaagc tatttttggt ataatattaa taaactgtaa
     8101 agaataagtt gaagaaggtt tatttctcag ttgacttata agataggaga ttcatgaaat
     8161 tactttatac tgatattaat catgatatga cagaaatctt agtcaatcag gccgcgcatg
     8221 ctgctgaagc tggatggcgt attttttata ttgctccaaa ctctttatct tttgaaaagg
     8281 agcgtgcagt attagagacc cttcctcaag aagcctcttt tgctattaca attactcgct
     8341 ttgctcaatt ggcacgttat tttactttaa accagcctaa tcaaaaagaa agtttaaacg
     8401 atattggatt agcaatgatc ttttatcgtg ctttagcaag ttttgaagat ggacaattaa
     8461 aagtttttgg acgattgaag caagatgctt cctttatcag tcaattggta gacctataca
     8521 aagaattgca gacagcaaat ctgagtattt tagatttaaa atatttacat tctcctgaaa
     8581 aatttgaaga tctattagct atttttctag tggtttcaga tcttttaaga gaaggagaat
     8641 acgacaatca atctaaaatt gcctttttta cagaacaagt aaggtcagga cagctagatg
     8701 tggatttaaa gaacactatc cttatagtcg atggttttac ccgtttctca gctgaagaag
     8761 aagctttaat taaaagtctg agttcacgtt gtcaagagat tatcatcgga gcttatgcta
     8821 gccaaaaagc gtataagact aactttacca atggaaatat ttattctgca ggggttgatt
     8881 ttttaagata tctagcaacg acatttcaaa caaaaccaga atttatcttg tctaagtggg
     8941 aaagtaagtc tggttttgaa atgatttcga aaaatatcga aggtaaacat gattttacaa
     9001 atagtagtca cattttagac gacactgcta aagattgtat aacaatttgg gagtgcatta
     9061 atcaaaaaga tgaagtggaa catgtggcta gagctatacg tcagaaactt tatcagggat
     9121 atcgctacaa agatatttta gttttactag gagatgtaga tagttataaa ctacaattaa
     9181 gtaagatttt tgaacagtat gatattcctt attatttcgg taaagctgaa acaatggctg
     9241 cgcacccttt agttcatttt atggattcct tgtcgcgaat aaaacgttat cgatttagag
     9301 ctgaagatgt tttaaatctt tttaagacag gtatttatgg agagattggt caagatgatt
     9361 tagattattt tgaagcttat atctcatatg cagatattaa aggttctaag aagttcttta
     9421 cagattttgt tgtgggggct aaaaaatttg atttagggcg tcttaacact attcgtcagt
     9481 ccctccttac tccactggaa agttttgtta aaacgaaaaa acaagacggt ataaaaacac
     9541 tcaaccaatt catgtttttc cttactcagg taggtttgag tgacaattta tcgcgattag
     9601 ttggtcaaat gtctgaaaat gaacaagaaa aacatcaaga agtttggaaa acatttacag
     9661 atattttaga gcagtttcaa acaatatttg gtcaagaaaa attaaattta gacgaatttt
     9721 tatcgttatt aaattcaggt atgatgcaag ccgagtatcg tatggtccct gctactgttg
     9781 atgtggttac tgttaagtct tacgacttgg tagaaccaca tagtaatcaa tttgtctatg
     9841 ccctaggcat gacacaatca cattttccaa agattgctca aaacaaatct ttaatatctg
     9901 atattgaacg tcagcttata aatgatgcca atgacactga tggtcatttt gacattatga
     9961 cgcgagaaaa tttgaagaaa aaccattttg ctgctctctc tctttttaat gctgctaaac
    10021 aagcattagt gttgactata ccacaattat tgaatgagtc tgaagatcag atgtcaccat
    10081 atcttgtcga actaagagat ataggtgttc cttttaatca taaaggacgc caatcgttaa
    10141 aagaagaagc tgataatatt gggaattaca aagctttatt atcgcgtgtt gttgacttgt
    10201 atcgctctgc tattgacaaa gagatgacta aagaagaaca aactttttgg tcagttgctg
    10261 ttcgttattt gcgtcgtcaa ttaacttcta aagggatcga gataccaata attacagata
    10321 gtcttgatac tgtgacagtt tcatcagatg tgatgaccag acgcttccca gaagatcacc
    10381 cacttaaatt atcctcatct gctttgacaa ctttttataa taatcaatat aagtattttt
    10441 tacaatatgt gttaggtttg gaagaacaag actcaattca tccagatatg cggcaccacg
    10501 gaacgtatct tcatcgtgtt tttgaaattc tgatgaaaaa ccaagggata gaatcttttg
    10561 aagagaaact caactctgca attaataaaa ccaatcaaga agatgttttc aaatccttgt
    10621 attcagaaga tgcagaaagt cgttattctt tagaaatttt agaagatatc gcgcgtgcaa
    10681 ctgcaaccat attacgacaa gatagtcaga tgactgttga aagtgaagaa gaacgatttg
    10741 aactaatgat tgataatact ataaaaatta acggtattat tgaccgtatc gatcgtttat
    10801 ctgatggttc tttgggtgtc gtggactata aatcaagcgc tcaaaaattc gatatccaaa
    10861 aattttataa cggtctaagt ccacaattag tcacctacat tgatgctatc agtcgtgaca
    10921 aggaagttga acaaaaacca ccgatttttg gagctatgta cttacatatg caagaaccta
    10981 gacaagactt gtctaaaatt aaaaatttag atgatttagt aacaaaaaat catcaagctt
    11041 taacttataa aggattattt tcagaagccg aaaaagaatt cttagcaaat gggaaatacc
    11101 atctcaaaga ttccctttat tcagaggctg aaattgctat tttacaagca cataatcaat
    11161 cgctttacaa aaaagcatct gagactatta agtcaggtaa gtttttgatc aatccatata
    11221 cagaagatgc taaaacagtt gacggtgatc agtttaagag tattacgggg ttcgaggcgg
    11281 ataggcatat ggcacgtgcc agagcgcttt ataaattacc tgctaaggaa aaacgtcaag
    11341 gtttcttaac attaatgcag caagaggagg aaaatgatga cctttaagcc tttcttaaat
    11401 ccagaagata ttgctgttat tcagacagaa gaaaaaaatt ctgataaaaa acaaaagcga
    11461 acgcctgaac aaattgaagc aatttatact tttggtaaca atgtcctggt atcagcttcg
    11521 gctggttctg gaaagacttt tgtaatggtt gagcggattt tagataaact tttaagaggg
    11581 gttccgattg agtctttgtt tatctcaaca tttacagtaa aagcggcagg tgagctcaaa
    11641 gaacgtttag aaaagaaaat aaacgagtct ttgaaatccg cagaatcaga tgatttaaaa
    11701 cagtttctca cccagcaact tgtcggcatt caaacagctg atattggtac tatggatgct
    11761 tttacgcaaa aaatagttaa ccaatatggt tatactttgg gcatatcacc tatatttagg
    11821 attttacaag ataaaaatga gcaagacgtc ataaagaatg aggtatatgc tgatttattt
    11881 tctgactaca tgactgggaa gaatgcagct tcattcataa aattagtcaa aaatttctca
    11941 ggtaatcgta aagatagcaa agcttttcga gaaatggttt ataaagttta tgcttttagc
    12001 cagtccatcg ataaccccaa gcgctggatg caaacagttt ttctaaaagg tgcacaaact
    12061 tacactgatt ttgaagctat tccagatcaa gaagtttcaa gccttttaaa tgtaatgcaa
    12121 acaactgcta atcaattacg tgatttaacg gatcaagaag attataagca attaactgct
    12181 aaaggggttc cgacagcaaa ttacaaaaaa caccttaaaa taattgaaaa tttagtccat
    12241 tggtctcaag actttaattt gctgtatgga aaaaaaggat tggctaattt ggcacgtgat
    12301 atcactaatg tgattccttc aggcaatgat gttacagttg ctggcgttaa atatcctatc
    12361 tttaaacaat tgcataatcg tatcgttgga cttaagcact tagaagttat ttttaagtat
    12421 caaggagaat ctcttttctt gttagaatta ttgcaatcct ttgttttaga tttctcagaa
    12481 caatatttac aggagaagat tcaagaaaat gcttttgaat tttctgatat tgcccatttt
    12541 gcaattcaaa tattggaaga aaatcatgat attaggcaaa tttatcaaga taagtatcat
    12601 gaagtaatgg ttgatgagta tcaggacaat aaccataccc aagagagaat attagaatta
    12661 ttgtctaatg gacataatcg tttcatggtt ggagacataa aacaatcaat ttaccgtttt
    12721 cgtcaggctg atccacaaat ttttaatgat aaatacaaag cttaccaaga caatccaagt
    12781 caagggaaat taattattct gaaagagaat tttcggagtc aatcagaggt tttagacagt
    12841 acaaatagtg tgtttacaca tcttatggat gaagaagtag gagacattct ttatgatgaa
    12901 agtcaccaat taaaagctgg tagtccaaga cagcaagaaa gacatccgaa taataaaaca
    12961 caagttttac tattagatac tgatgaagat gatatagatg acagtgatag tcaacaatat
    13021 gatattagcc cagctgaagc aaagttagtc gcaaaagaaa ttattcgatt gcataaagaa
    13081 gagaatgttc cctttcaaga tatcaccctc ttagtttctt caaggacaag gaatgatggt
    13141 atcttacaaa cttttgaccg ttatggtatt cctttggtaa ctgacggtgg ggagcaaaat
    13201 tacttaaaat cagtcgaagt gatggtaatg cttgatactt tgcgatcaat tgataatccc
    13261 ttaaatgatt atgctctagt tgctcttttg agatctccca tgtttggctt taatgaagat
    13321 gatttaacaa gaattgctat tcatgatgtc aaaatggctt tttatcataa agtgaaattg
    13381 tcttatcata aagaaggtca tcatagtgat ttaattactc cagaattatc ttctaaaata
    13441 gattacttta tgaagacatt ccagacttgg cgtgattttg ctaaatggca ttcgctttat
    13501 gatttaatat ggaaaatata taatgaccgt ttttattatg attatgtagg agctctgcct
    13561 aaagctgagc aacgtcaagc caatctatat gccttggcac ttcgagcaaa tcagtttgaa
    13621 aaaacaggtt tcaagggttt atcacgtttt attcgaatga ttgataaagt tttagaaaat
    13681 gaaaatgatt tggcagatgt tgaggtggca ttaccgcaaa atgctgttaa cctaatgact
    13741 atacacaaat caaagggtct tgagtttaag tatgtattta ttttaaatat tgataagaaa
    13801 ttttcaatgg tagacataac ttcccctctt attttaagcc gaaatcaagg aattggtatt
    13861 aaatatgttg ctgatatgcg acatgaactc gaagaagaga tactgcctgc agtaaaggtt
    13921 agcatggaaa cacttcctta ccaacttaat aaaagagagt tacgcttagc tactttatca
    13981 gagcaaatgc gtttacttta cgtagctatg acacgtgcag agaaaaaatt atatctggtt
    14041 ggtaaggcta gtcaaaccaa atgggctgat cattatgact tagtatctga aaataattat
    14101 ctcccattag ctagtcgtga gacttttgtg acctttcaag attggttact ggctgttcat
    14161 gaaacctata agaaacaaga gttgttttat gacatcaact tcgtatcatt agaggaatta
    14221 acagatcacc acatagggat ggttaatcct agtctacctt tcaatcccga taataaggca
    14281 gagaatcgtc agtctgaaga tatcgtgcac gcgattagcg tgttagaatc tgttgaacag
    14341 attaatcaaa cttataaagc ggctattgag ttaccgacag taagaacacc aagtcaagtc
    14401 aaaaaattct atgagcctat tctagatata gaaggcgttg atgtgatgga aactattact
    14461 aaaacatcag tagattttaa gttacctgat ttttctacaa gtaaaaagca agacccagca
    14521 gctttaggat ctgctgtaca tgaattaatg caacgcatag agatgtcatc tcatgtcaaa
    14581 atggaagata ttcaaaaagc cttgacagaa gtgaatgctg aaacaagtat caaagcggct
    14641 attcagattg aaaaaatcaa ctattttttc caagaaaact cactcggtaa atatatacaa
    14701 gaagaagtag aacatctaca tcgagaggca ccttttgcca tgttaaaaga agatccggag
    14761 agtggtgaga aattcgttgt acgtggtatt attgatgggt atctactatt agaaaatcgg
    14821 ataattctat ttgattataa gactgataaa tttgtcaatc ctctggaatt aaaagaacgc
    14881 tatcaggggc aaatggcttt atatgcagaa gctttaaaaa aatcttacga gatagaaagg
    14941 atagacaaat acttaattct tttaggtggt aaacagctag aagtggtaaa aatggattaa
    15001 agaaaggaaa aaatgatagt cgaacaaaaa tttggaaatg gttttacttg gattaacatt
    15061 gaagcagagc aattgcgaac agaaacatcg gaaattcaag caaaatattt ggatagtgag
    15121 attattacat atgctctgga tgattatgaa cgagccttta tggaatgttc tcatattaaa
    15181 ggaaaagaag tcttgacgat tatttttaat actattgatc taaagcagaa agaaagttat
    15241 tatgagaccg ttccgatgac cttttgctta tcacacgacc gtttgataac ggtgacacgg
    15301 tctcgaaata gttatatgct agaactattg caaaaatatc tagaccgtaa tccagatgtc
    15361 tctcctaaaa aatttttatt tgcagcttta actttgatta caaaacaata ttttaatgtt
    15421 gtatcgaaaa ttgatagaga aaaggatatt ctcaaccgtc aattgagaga acaaacaacc
    15481 aataagcgct tattagccat gagtgaccta gagacaggct ccgtttatct tttgacagct
    15541 gccaatcaaa atgcattggt attagagcaa ttagatgttc atcctagtca gcgatttaat
    15601 tctgaagtgg aaaaagaaca gttatcagat gctctcattg aagcgcatca gttagtcagt
    15661 atgacacaat taaacagtca ggtactttca caattatcat ctacttttaa taatgtctta
    15721 aataataact tgaacgaaaa cttaacaggt ttgaatatca tttcgattaa tttagcgatt
    15781 attgcagcaa ttactggctt ttttgggatg aatattccat tacctcttac agaatcacgg
    15841 agttcatggt taattgttat tgctacttcg gtcttacttt gggttattat tgctcaaatt
    15901 ttaaaacgag tttttaactt aagaaaataa aaatgttcag tgcgtaagca ccgaacattt
    15961 tttatttacc taagcagaat tggctgaaga gctgagttat tagctcatct ggagctgcat
    16021 ccccagtgat ttctccaagt atttcccaag ttctagtcat atcgacttgt aagagatcaa
    16081 caggcattcc taattccaag ccctcattga cagcttttaa actatcaacg gctttttcaa
    16141 ttagtgagat atggcgagca ttcgatagat aagtagcgtc atgttctacc ataccagcat
    16201 tgtcaaagaa aatatcgtta atacgttctt cgattttatc gatgttttca ttttctaaaa
    16261 ccgaaattgg aataacattt tcaggtaatt cgttaacttc tattttttga ggaagatccg
    16321 ttttattgag taatacgata cgattacttt ctttacttag ttctagaagg gagcggtctt
    16381 gtaatgttag tggttcggaa ctattgagaa ccaacaacac aaggtcggct tcttctaaag
    16441 ccttctttga acgttcaaca ccaatttttt caacgatatc atctgtatca cggataccag
    16501 cagtgtcaac tagttttaaa ggaactcctt taatattgac gtattcttca ataacatcac
    16561 gagttgttcc ttcaatatct gtcacaatcg ccttttcttc acgtaagagg ttatttagta
    16621 agctggattt accgacattt ggacgaccaa tgattgctgt tgaaagtcct tctcgaagaa
    16681 tttttccacg tcgggcagtt cgaagtaaat tttccataag tgcttgaaac tcttgtgttt
    16741 tttcacgcat cagtgttgta gtcatctctt caacgtcatc atattcaggg taatcgatat
    16801 taacttcaac ttgggctaga gtatttagaa tttcttgtcg agtgttattg attagtgtct
    16861 ttaaagaacc atctaattgc ttaacggcaa tatccattgc tttatctgtt ttagctctaa
    16921 taagatccat aacagcttca gcctgagtca agtcaactcg accgtttaaa aaagcacgtt
    16981 tagtaaattc tcctggttca gccattcgtg ctccatgtcg taaaattaac tgaagaattt
    17041 cgttagtaac tgcaattcct ccatgcgtat taatttcaat aacatcttct cttgtgaatg
    17101 tttttggagc aagcataacg cctaacataa cttcatccaa gatttcattt ttatctgggt
    17161 ccacaatatg accatagttt aaggtatggg attggatagc ggatagatct tttccacgat
    17221 aaatttttga tgctatcttt aaggcatctg taccagatat acggacaata ccaatagctc
    17281 cctcaccgag aggagttgaa atagctgcga tggtatcaaa ttcttttgta atagacataa
    17341 agtctctctc tttcttggga attactaaca ttcattgtaa cctaaagccc taaaaggtgc
    17401 aatcatttga ttaaaagaga agatttccat aatggtttta tttgaaggta tgctataata
    17461 aaactatgat tatactacaa ggaaacaaaa ttgagcgctc attttcaggt gatgtgcttt
    17521 ttgataatat taatattcaa gttgatcaac gtgatcgtat tgctcttgtt ggacgtaatg
    17581 gtgctggcaa atcaacgctt cttaaaattt tagttggtga agaggctccg acaaaaggag
    17641 aaataaataa aaagcgtgat ttgagtttgt cctacttggc acaagatagt cgttttcaat
    17701 ctgaaaatac aattttccaa gaaatgttac aagtttttga ttcattaaga gaagtagaga
    17761 agcgcttacg tgaattggaa ttacaaatgg gacaagtttc aggaagtgac ctggaacaat
    17821 tgatgaaaac atacgatatt ttgtcagaag agtttcgtga aaaaggtggc tttacctatg
    17881 aatcagatat aaaggctatt ttaaatggct ttaagtttaa cagtgatatg tgggaaatgc
    17941 ctattagtga attgtctggt gggcaaaata cacgattagc gcttgcaaaa atgctacttg
    18001 aaaagccaga attacttgtt ttagatgaac caacgaacca tttagacatt gatacgattg
    18061 cttggttaga aaattatctt gtaaattatc aaggagccct tattattgtc agccatgacc
    18121 gctatttttt agacaaggta gctacagtaa cttatgattt aacgacacat tctctagacc
    18181 gctatgtagg caattattcg aaatttatgg atcttaaggc agagaaaata gcgactgaag
    18241 aaaagaattt tgaaaagcaa caaaaagaaa ttgctaagtt agaagatttt gtacaacgta
    18301 acattgttag ggcttcaacc actaaacgag cacaagctcg tcgtaaacaa ttagaaaaga
    18361 tggaacgact cgacaaaccg aatgtagagc aaaaatcagc taatatgact tttcatgctg
    18421 ggaaagtctc agggaatgtt gttttaacac ttgagaatgc agctattggt tatgaaggtg
    18481 tttctttatc ggagccaatc gatttggacg tgaagaaatt tgatgctatt gcaattgtag
    18541 gtccaaatgg tatcgggaaa tcaactttaa tcaaatcgct tgttgggcaa attcctttta
    18601 tcaaaggaga agcaaaatta ggagcaaatg tcgaaactgg gtattacgat caaagtcaaa
    18661 gtaatttaac aaaaacgaat actgttttag atgagttatg gaatgctttt tcaacaactc
    18721 cagaagttga aattagaaac cgtttaggag cttttctttt ttcaggtgat gatgttaaaa
    18781 aatcagtttc aatgctatcc ggtggtgaac gagcgcgttt gctattggca aaattatcta
    18841 tggaaaataa taatttcctc atattagatg agccaacaaa ccacttggat attgatagta
    18901 aagaagtctt agaaaatgca ctcatcgaat ttgatggcac attacttttt gttagtcatg
    18961 atcgctattt tattaatcgt gtggcaacta aggttctcga aatttctgat aagggaagta
    19021 ctctctatct aggagattat gattattacc tcactaagaa agccgaacta gaggaattag
    19081 cccgtttaaa tgaagaagaa gtgagtgctt caaaaacaga aatcgacgtt acatcagatt
    19141 atgagacaca aaaagcaaat caaaaggaat ttaggaaaat tacccgtcgc gttgcagaaa
    19201 ttgaggcacg tttggaagtt cttgaaaatg atgaaaacaa tatcaatggt ttaatgttag
    19261 aaaccaatga catcggaaaa ctctctgatt tacaaaaaga gctggaatca atccaagaag
    19321 aacaattgct attaatggaa gaatgggaaa atctaaatat gagactagat ttgtgagaaa
    19381 atttcaaaaa caatagtttg tgaaaaaaag tacattttaa attgagatgc cttttacgaa
    19441 aacaaagaaa gcggttcaaa ttcttgcatc ttgtgaaaaa aacttataca atgattaagt
    19501 aataaagatt tttttggagg ttagaatggt aactctatct aaagaacaac acttggatat
    19561 gtttctaaaa atgcaacgta ttcgtgatgt tgatatgaaa tttaacaaat tggttcgtcg
    19621 tggtttcatc caagggatga ctcacttctc tgttggtgaa gaagctgctt cagtaggtgc
    19681 tatccaagat ttaacagatt cagatatcat tttctcaaac caccgtggac acggacaaac
    19741 tattgcaaaa ggtattgata ttggtggaat gtttgctgaa cttgctggta aagcaacagg
    19801 aacatcaaaa ggtcgtggag gctcaatgca cttagcaaac cttgaaaaag gtaactatgg
    19861 aacaaacggt atcgtcggtg gaggatatgc gcttgcggta ggtgctgccc ttacacagca
    19921 atatgaagga actgataaca ttgttatcgc cttttcgggt gactcagcga ctaacgaagg
    19981 ctcattccat gagtcagtta acttagctgc tgtttggaac ttaccagtta tcttctttat
    20041 catcaacaac cgttatggta tctcaacaga catcacatac tcaactaaaa ttcctcacct
    20101 ttacatgcgt gctgatgcgt acggtattcc agggcattat gttgaagatg gtaatgatct
    20161 tatggctgtt tacgagaaaa tgcatgaagt tatcaattac gtgcgttcag gaaatggacc
    20221 agctattgta gaggttgaat cataccgttg gtttggtcat tcaactgctg atgctggggt
    20281 ataccgtact aaagaagaag ttgattcatg gaaagctaaa gatccagtaa aacgctaccg
    20341 tgcatactta attgaaaatg aaatcgctac tgaagaagag cttgcagcta ttgaagcaca
    20401 agtgattaaa gaagttgaag aaggggttaa attcgctgaa gaaagtccat tccctgatat
    20461 gtctgttgca tttgaagacg tttttgtgga ctaaaataac ctaaagtaga tgagatttat
    20521 ctcgttctat tattcaattg attaaaaaac ggatattcgg aggaaataat gagcgaaact
    20581 aaagttatgg ctttgcgcga agcgattaat gtcgctatga gcgaagaaat gcgcaaagat
    20641 gaaaaagtat ttttaatggg tgaagatgta ggtgtatatg gaggagactt cggtacatct
    20701 gttggtatgt tagaagagtt tggtgctaag cgcgttcgcg acacaccaat ctcagaggct
    20761 gcgattgcag gttcagctat cggtgctgca caaacaggtt tgcgtcctat cgttgatttg
    20821 acattcatgg atttcgtaac aatcgcaatg gatgctatcg ttaaccaagg tgctaaaacg
    20881 aattatatgt ttggtggagg tctatcaact ccagttactt tccgtgtggc atcagggtca
    20941 ggtattggtt cggctgcaca acactcacaa tcattagaag cttggttaac acacattcca
    21001 ggtctaaaag ttgttgcacc tggtactgtt aatgagtcaa aagcactttt gaaatcttct
    21061 attcttgaca ataacccagt tatcttctta gagccaaaag ctctctatgg taagaaagaa
    21121 gaagttaata tggatcctga tttctatatt ccacttggta aaggggatat taagcgtgaa
    21181 ggtactgact taacaattgt ttcttatggc cgtatgcttg agcgtgttat gcaagctgct
    21241 gaagaagttg ctgaggaagg tattaacgtt gaggttgttg atccacgtac acttattccg
    21301 cttgataaag aattaattat cgattctgtt aagaaaactg gtaaattgat cttggttaac
    21361 gatgcttata aaactggtgg tttcactggt gaaattgcaa ctatggttgc tgaaagtgaa
    21421 gcatttgatt accttgatca tcctatcgtg cgtcttgcaa gtgaagatgt tccagtaccg
    21481 tattcacgtg tacttgaaca aggaatctta cctgatgtag caaaaattaa agatgctatc
    21541 tacaaagtag ttaacaaagg taaataattc ataaaacaaa gagggactga ggaatgagtg
    21601 aaaacttagc ctctttcctt gtttataggt tttaatttat atcgttagat gaaacatata
    21661 tttgacgata tagtttagtg tgaaaggaaa actatggctg tcgaaattat tatgcctaaa
    21721 ctcggggttg acatgcaaga aggcgaaatc cttgagtgga aaaaacaggt aggtgatgtt
    21781 gttaatgaag gtgacgtcct acttgaaatt atgtcagata aaacaaatat ggagattgaa
    21841 gcagaagact caggtgtttt actaaaaatc acacatggga atggagatgt tgttcctgta
    21901 actgagacaa ttggctgtat cggtgctgaa ggtgaagaag taacagaagc atcttcttct
    21961 gaaaatacat cagttgaaga aaacgcgaca caagtaactt cagaaccaga aaaagttgaa
    22021 gaaacatcag aaccaagtgt gccagcagct acaagtggtg aaaaagtacg tgccactcct
    22081 gcagctcgta aattagcaag agagatgtct attgaccttg cactagtatc tggaacaggg
    22141 gcaaatggtc gcgttcacag agaagatgtt gaaagcttca agggtgctca accacgtatt
    22201 acaccattgg cacgtcgaat tgctgaagat caaggagttg atatagcaga aatcactggc
    22261 agtggtattc gtggtaaaat tgttaaaaat gatgttttgg ctgctatgtc accgcaagca
    22321 gcagaggctc ctgtaaaaac gaaagctaca ccaacaacag aagaaaaaca attaccagaa
    22381 ggtgttgaag tcattaagat gtctgcaatg cgtaaggcta tttctaaagg tatgactaac
    22441 tcttacctca ctgctccaag ctttacactt aactatgata ttgatatgac tgaaatgatg
    22501 gctcttcgca agaaattaat tgatccaatc atggctaaaa caggcttgaa agtcagcttt
    22561 actgatttga ttggaatggc cgttgttaag acattgatga aaccagagca tcgataccta
    22621 aatgcttcat taattaacga tgcacaagaa attgagcttc ataattttgt taatattgga
    22681 atcgcagttg gtcttgatga tggtttgatt gttcctgttg ttcacaatgc tgatcaaatg
    22741 tcactttctg actttgttat agcatctaaa gatgttatta agaaaactca agaaggtaaa
    22801 ttaaaatcag ctgaaatgtc aggttcaaca ttctcaatta ctaacttagg tatgtttggt
    22861 actaagacgt ttaacccaat tatcaatcaa ccgaactcag caatccttgg agtaggtgct
    22921 actattccga ctccaacagt ggttgatggt gaaattgtag ctcgaccaat catggcaatg
    22981 tgtttaacaa ttgaccaccg tattgttgat ggaatgaatg gtgctaagtt tatggttgat
    23041 cttaagaact taatggaaaa cccatttggt ttacttattt gattccgtag gtcttaattt
    23101 gatataataa gttattcgaa aaacgaaaga ggaaataaaa aatggctttt gatgtaatta
    23161 tgccaaaact tggggttgac atgcaagaag gcgaaatcct tgagtggaaa aaaaatgaag
    23221 gtgacaccgt taatgaaggt gatgtccttc ttgaaatcat gtctgataaa accaatatgg
    23281 aaattgaagc agaagacact ggtgtacttt taaaaattgt acatcaggca ggagatgttg
    23341 ttcctgtcac tgaagtgatt gcctatatcg gagaagaagg tgaagaagta ggtacgtcat
    23401 caccttcggc tgatgcaact atcacagctg aagatggtca atcagtttct ggacctgctg
    23461 ctccttcaca agagacagtt gcagcagcaa ctcctaaaga agaacttgca gctgatgagt
    23521 acgatatcgt agtcgtaggt ggtggccctg caggttatta cgcagcaatc cgcggtgctc
    23581 aacttggagg aaaaattgcc attgttgaga aaactgaatt tggcggtacg tgtttgaatg
    23641 ttggttgtat ccctacaaaa acatatctta aaaatgctga aatcctagat ggtttgaaag
    23701 tagcagctgg acgtggtatc aaccttgctt caactaacta tgcaattgat atggataaga
    23761 ctgttgcctt taaaaattct gtagttaaga cattgactgg tggtgtacgt ggtctcttga
    23821 aagcgaacaa agttgaaatt ttcaatggac ttggacaagt aaaccctgat aaatctgttg
    23881 ttattggcga taaagttatc aaaggtcgta acgtcgtcct tgcgactggt tctaaggtat
    23941 ctcgtattaa tattccaggt attgaatcac cattagtatt aacatcagat gatattcttg
    24001 atttacgtga aattcctaag tcacttgctg ttatgggtgg tggtgtcgta ggtattgagc
    24061 ttggtcttgt atgggcttca tatggtgttg acgtaactgt tattgaaatg gctgatcgca
    24121 tcattccagc aatggataaa gaagtatcgc tagaacttca aaaaatcctt gctaagaagg
    24181 gaatgaagat taaaacatca gttggtgttt ctgaaattgt tgaagccaat aatcaattaa
    24241 cgcttaaatt aaacaacggc gaagaagttg ttgctgataa agctcttctt tcaattggac
    24301 gtgttccaca aatgaatggt cttgaaaatc ttgagcctga acttgagatg gaacgtggac
    24361 gtattaaagt taatgcttat caagaaactt caattccagg tatctatgca ccaggtgatg
    24421 ttaatggaac tcgtatgtta gctcatgctg cataccgtat gggtgaagta gctgctgaaa
    24481 acgcactcgg cggtaataag cgtaaagcac atctagattt cactccagca gctgtttata
    24541 ctcatcctga agtagcaatg gtggggatga cagaagagca agcacgtgaa caatatggtg
    24601 acatattagt tggtaaaaat agctttacag gcaatggtcg tgctatcgct tcaaacgaag
    24661 cacatggttt tgtaaaagta attgctgaac ctaagtataa agagattctt ggtgttcaca
    24721 ttattggtcc agctgcagcg gaattgatta atgaagcttc tacgattatg gaaaatgagt
    24781 tgactgtcta tgatgtagca caatctatcc atggtcaccc aacattctct gaagttatgt
    24841 acgaagcttt cttagatgtt ctcggtgaag ctattcacaa ccctccaaaa cgcaaataat
    24901 tttgtttaat atatttgaga ttgagctttt atagcttcaa tctcttttca cgtatgtatt
    24961 gtctgaatat tataaaatat tagttaggaa tttaaaatga aatatatcgt taacacgtct
    25021 aatgatccgg cttacaatgt tgctttagaa gcttacgcct ttcaaaagtt gacagatatc
    25081 gatgaaatct ttattctttg gattaatgaa ccggctatca ttattggacg ccatcaaaat
    25141 acaattcaag aaattaacaa agagttcatt gataaaaatg gcatccatgt tgtccgtcgt
    25201 ttgtcaggag gcggagcagt ttatcatgac ttgaataatt taaactacac tattatttct
    25261 aataatacac aagaaggtgc ttttgatttc caaacttttt caaaaccagt tattgatact
    25321 cttgctaaat taggtgttaa agcagaattt actggtcgta atgatttaga aatcaatgga
    25381 caaaaatttg caggaaatgc tcaagcttat tacaaaggtc gtatgatgca tcatggttgt
    25441 cttttatttg atgttgatat gtcagtctta gggcaagcac tcaaagtatc taaagacaaa
    25501 attgaatcta aagggataaa atctgtacgc gcacgtgtta caaatattgt tgaccatctt
    25561 tcagataaaa ttacagtaca agaatttagc gatgccattt tagctcaagt gaaggaagaa
    25621 tatccagaga tggatgaata tgttttgagt gatgccgagt tatcagaaat ccaagctatg
    25681 cgcgataatc aatttgctac atgggattgg acgtatggta aagcacctga atacaccatt
    25741 gaacgtggtg tccgctatcc agctggaaaa attacgactt atgctaatgt tgaaaattca
    25801 acgataaaat ctgtcaaaat ctttggtgac ttctttggag tgaaacctgt agatgatatt
    25861 gaaaaaatgt tggaaggtgt ccgctacgat tacaaagatg ttttagcagc gcttaaaaca
    25921 gttgatacaa gtcaatactt ctcacgtatg acacccgaag agattacaaa agctattgta
    25981 gactaaaatc aaaaaaccta gtcaaagtct ttgcctttgg ctaggttttt tgtatggtta
    26041 agtggcctta tagtacgacg ggcatgtcgt acatctgagg tgcaagtcct ctgtgggcac
    26101 ccgctaccgg tgaacccaat agcgactccc aagcttgact atcgtgaggt agcggggaga
    26161 ggaagggata gcgaaatc
//
LOCUS       DATRQA010000010        25759 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.010, whole genome shotgun sequence.
ACCESSION   DATRQA010000010 DATRQA010000000
VERSION     DATRQA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25759
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.010"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(298..684)
                     /gene="rplQ"
                     /locus_tag="VBD27_000312"
     CDS             complement(298..684)
                     /gene="rplQ"
                     /locus_tag="VBD27_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO7024349.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEASDKYTSTTALQKLFDDIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(699..1637)
                     /locus_tag="VBD27_000313"
     CDS             complement(699..1637)
                     /locus_tag="VBD27_000313"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO7024350.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKIKLADLGLGLKNDK"
     gene            complement(1687..2070)
                     /gene="rpsK"
                     /locus_tag="VBD27_000314"
     CDS             complement(1687..2070)
                     /gene="rpsK"
                     /locus_tag="VBD27_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO7024351.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2088..2453)
                     /gene="rpsM"
                     /locus_tag="VBD27_000315"
     CDS             complement(2088..2453)
                     /gene="rpsM"
                     /locus_tag="VBD27_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO7024352.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLSTSKKILAAAGISEDIR
                     VKDLTPDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2471..2587)
                     /gene="rpmJ"
                     /locus_tag="VBD27_000316"
     CDS             complement(2471..2587)
                     /gene="rpmJ"
                     /locus_tag="VBD27_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO7024353.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2613..2831)
                     /gene="infA"
                     /locus_tag="VBD27_000317"
     CDS             complement(2613..2831)
                     /gene="infA"
                     /locus_tag="VBD27_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO7024354.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(2947..3585)
                     /locus_tag="VBD27_000318"
     CDS             complement(2947..3585)
                     /locus_tag="VBD27_000318"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO7024355.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTQ
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPTCLIERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPILEHYRKLGLVTDIEGNQEITEVFADVEKALLELK"
     gene            complement(3680..4984)
                     /gene="secY"
                     /locus_tag="VBD27_000319"
     CDS             complement(3680..4984)
                     /gene="secY"
                     /locus_tag="VBD27_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO7024356.1"
                     /translation="MFLKLLRDALKVKMVRNKILFTIFILLVFRIGTHITVPGINVKS
                     LEQMGELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLFLAFVQSIGITAGFNTLSSVALVKTPNVQTYLLIGAILTTG
                     SMVVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAITTIYEDFFVNVRSSAITNSYIF
                     VGILIVAVLAIVFFTTFIQQAEYKIPIQYTKLVQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFFQNGKEIPWLTKLQELLNYQTPVGMIIYAILIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSIRPGRETEEYMSSLLKKLATIGSVFLAFISLLPIIAQQALHL
                     SSSIALGGTSLLILIATGIEGMKQLEGYLLKRRYVGFMNTTE"
     gene            complement(5005..5445)
                     /gene="rplO"
                     /locus_tag="VBD27_000320"
     CDS             complement(5005..5445)
                     /gene="rplO"
                     /locus_tag="VBD27_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO7024357.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRMPKRGFSNINAKEYALVNLDQLNVFEDGTEVTPVVLKEAGIV
                     RAEKSGVKILGNGELTKKLSVKAAKFSKSAEAAITAKGGSIEVI"
     gene            complement(5570..5749)
                     /gene="rpmD"
                     /locus_tag="VBD27_000321"
     CDS             complement(5570..5749)
                     /gene="rpmD"
                     /locus_tag="VBD27_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO7024358.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVNAISHLVTVEEA"
     gene            complement(5764..6258)
                     /gene="rpsE"
                     /locus_tag="VBD27_000322"
     CDS             complement(5764..6258)
                     /gene="rpsE"
                     /locus_tag="VBD27_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO7024359.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMVEVPMVGTTIPHEVRSEFGGAKVLLKPAVEGA
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6277..6633)
                     /gene="rplR"
                     /locus_tag="VBD27_000323"
     CDS             complement(6277..6633)
                     /gene="rplR"
                     /locus_tag="VBD27_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO7024360.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSNGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADSARENGLKF"
     gene            complement(6734..7270)
                     /gene="rplF"
                     /locus_tag="VBD27_000324"
     CDS             complement(6734..7270)
                     /gene="rplF"
                     /locus_tag="VBD27_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO7024361.1"
                     /translation="MSRIGNKVITLPAGVEIINKDNVVTVKGPKGELTREFNKNIGIT
                     VEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSK
                     LVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(7380..7778)
                     /gene="rpsH"
                     /locus_tag="VBD27_000325"
     CDS             complement(7380..7778)
                     /gene="rpsH"
                     /locus_tag="VBD27_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO7024362.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIADILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGQNGERVITNLKRISKPGLRVYTKHEDMPKVLNGLG
                     IAIVSTSEGLLTDKEARQKNVGGEVLAYIW"
     gene            complement(7933..8118)
                     /locus_tag="VBD27_000326"
     CDS             complement(7933..8118)
                     /locus_tag="VBD27_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEO7024363.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFQLCRVCF
                     RDLAYKGQVPGVTKASW"
     gene            complement(8136..8678)
                     /gene="rplE"
                     /locus_tag="VBD27_000327"
     CDS             complement(8136..8678)
                     /gene="rplE"
                     /locus_tag="VBD27_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO7024364.1"
                     /translation="MANRLKEKYTNEVVPALTEKFNYSSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(8702..9007)
                     /gene="rplX"
                     /locus_tag="VBD27_000328"
     CDS             complement(8702..9007)
                     /gene="rplX"
                     /locus_tag="VBD27_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO7024365.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVVVEGVALIKKH
                     QKPNNENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYNKKSGEVLD"
     gene            complement(9087..9455)
                     /gene="rplN"
                     /locus_tag="VBD27_000329"
     CDS             complement(9087..9455)
                     /gene="rplN"
                     /locus_tag="VBD27_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO7024366.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9480..9740)
                     /gene="rpsQ"
                     /locus_tag="VBD27_000330"
     CDS             complement(9480..9740)
                     /gene="rpsQ"
                     /locus_tag="VBD27_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO7024367.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(9766..9972)
                     /locus_tag="VBD27_000331"
     CDS             complement(9766..9972)
                     /locus_tag="VBD27_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO7024368.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(9982..10395)
                     /gene="rplP"
                     /locus_tag="VBD27_000332"
     CDS             complement(9982..10395)
                     /gene="rplP"
                     /locus_tag="VBD27_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO7024369.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGTPEGWVAPVKRGKV
                     MFEIAGVSEEVAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10399..11052)
                     /gene="rpsC"
                     /locus_tag="VBD27_000333"
     CDS             complement(10399..11052)
                     /gene="rpsC"
                     /locus_tag="VBD27_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO7024370.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11065..11409)
                     /gene="rplV"
                     /locus_tag="VBD27_000334"
     CDS             complement(11065..11409)
                     /gene="rplV"
                     /locus_tag="VBD27_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO7024371.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKNVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11425..11703)
                     /gene="rpsS"
                     /locus_tag="VBD27_000335"
     CDS             complement(11425..11703)
                     /gene="rpsS"
                     /locus_tag="VBD27_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO7024372.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(11802..12635)
                     /gene="rplB"
                     /locus_tag="VBD27_000336"
     CDS             complement(11802..12635)
                     /gene="rplB"
                     /locus_tag="VBD27_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO7024373.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAELIRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCSATIGTVGNEQQSLVNIGKAGRNRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            complement(12653..12949)
                     /locus_tag="VBD27_000337"
     CDS             complement(12653..12949)
                     /locus_tag="VBD27_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO7024374.1"
                     /translation="MNLYDVIKKPVITEKSMVALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVTVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(12949..13572)
                     /gene="rplD"
                     /locus_tag="VBD27_000338"
     CDS             complement(12949..13572)
                     /gene="rplD"
                     /locus_tag="VBD27_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO7024375.1"
                     /translation="MANVKLFDQTGKEVSSVELNEAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVENLSFAAPKTAEFASVLSALSIDSKVLVILEEGN
                     EFAALSARNLPNVTVATATTASVLDIVNADKLLVTKEAISTIEGVLA"
     gene            complement(13596..14222)
                     /gene="rplC"
                     /locus_tag="VBD27_000339"
     CDS             complement(13596..14222)
                     /gene="rplC"
                     /locus_tag="VBD27_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO7024376.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVVLIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14295..14603)
                     /gene="rpsJ"
                     /locus_tag="VBD27_000340"
     CDS             complement(14295..14603)
                     /gene="rpsJ"
                     /locus_tag="VBD27_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO7024377.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(14810..16090)
                     /locus_tag="VBD27_000341"
     CDS             complement(14810..16090)
                     /locus_tag="VBD27_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO7024378.1"
                     /translation="MRYSKEIIQLAIPAMIENILQMLMGVVDNYLVAQLGVVAVSGVS
                     VANNIITIYQAIFIALGASIASLLAKSLAGSEKDDAISVCSQAIFLTSLIGAVLGIIS
                     IVFGQTFFKLLGTTKSVAQVGGLYLAIVGGGVVTLGILTTLGSFLRVQGQPRLPMYVS
                     IFVNFLNAVLSGFAIFEWRYGLVGVAVSTLIARLIGICILAKYLPIKKIIKRMTWKIS
                     AQIWNLALPSAGERLMMRAGDVVIVAIVVQLGTNVVAGNAIGETLTQFNYMPGLGIAT
                     ATIILTAKYVGQKNRESIEETIQSSYYIGLVLMILISSFMLLAGKPLTQLFTNNPSAI
                     KGSLIVILLSFVGVPATIGTLVYTAAWQGLGNAKLPFYTTTIGMWLIRVVLGYLLGIV
                     FELGLLGVWMATIADNIFRWLFLKVRYHRYIQKM"
     gene            complement(16195..17685)
                     /locus_tag="VBD27_000342"
     CDS             complement(16195..17685)
                     /locus_tag="VBD27_000342"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEO7024379.1"
                     /translation="MAIVYQSTRDASNKVTASQAILKGLADDGGLFTPITFPKVDLDF
                     TKLKDASYQEVAKLVLSAFFDDFTEQELDYCISQAYDTKFDTTEIAPIVKIGDRYHLE
                     LFHGPTIAFKDMALSILPYLLTTAAKKQGVDDKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKHGVSYIQELQMITQAGQNTHVVAIEGNFDDAQTSVKEMFNNSLLRSKLSQ
                     QHMQLSSANSMNIGRLVPQIVYYIYAYAQLVKSKEISIGQLINFSVPTGNFGNILAAY
                     YASQIGLPVTKLICASNDNNVLTDFFKTQTYDKNREFKVTSSPSMDILVSSNLERLIF
                     HLLGDDAETTKKLMEDLVTTGEYALEARQANILESFVAGFATEQFVELDIKRLFDQYQ
                     YIEDPHTAVASAVYQAYQTETKDQTPTVIVSTASPYKFPCVVTKAITNKEEIQDFAAI
                     SILNDLSGVSLPKAVTDLQKAEVIHRMVVPTSNMRETVEECLGLKQ"
     gene            complement(17805..18821)
                     /gene="adhP"
                     /locus_tag="VBD27_000343"
     CDS             complement(17805..18821)
                     /gene="adhP"
                     /locus_tag="VBD27_000343"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEO7024380.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPKVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLRVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAIVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIAVYGAGGLGNLAVQYAKKVFNAHVVAVDINADKLQLAKEVGADLTVNGKEIK
                     DVAAYIQEKTGGCHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIRVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPQVFDEMERGLIQGRKVL
                     DFTK"
     gene            complement(18999..21641)
                     /gene="adhE"
                     /locus_tag="VBD27_000344"
                     /gene_synonym="adhC"
     CDS             complement(18999..21641)
                     /gene="adhE"
                     /locus_tag="VBD27_000344"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEO7024381.1"
                     /translation="MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVA
                     KASVAALDAHGELALHAVEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDV
                     TGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWIEQPSIDATNALMKHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKSAVWIAEQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHSAAIHTKD
                     ADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTDLMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDY
                     TDGLALQAIKIVFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPAATP
                     EEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYEERPGRRK"
     gene            complement(21910..23688)
                     /locus_tag="VBD27_000345"
     CDS             complement(21910..23688)
                     /locus_tag="VBD27_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO7024382.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFKNSFPGGFVGVDIFFTFSGFL
                     ITALLIDEFSKTKKIDFVSFCRRRFYRIFPPLVLMVLVTIPFVFLVKSDFRASIGSQI
                     MTALGFTSNFYEILTGGNYESQFIPHLFVHTWSLSIEVHFYVLWGLTVWLLSKRSKDQ
                     KQLRGTLFLVSMGVFGVSFLTMFVRAFFVDNFSTIYFSTLSHIFPFFLGAMVATISGI
                     REITGRFKKNIKNLTLKHNLIMMGSAFAGLMILTFALDFDNRLTYLFGFVLSSIFASV
                     MIYNARILHEHTPDISEPFVITYLADISYGMYLFHWPFYIIFSRLSPNWIAVILTVVL
                     SAVFSTLSFYIIEPFILGRKPKFLDYEFDLLPYKKWLFSIGGVLTLITVVTMLTAPSI
                     GSFETELLQNSLQQARTNTNRTHTLAAGDAGALSDVTVIGDSVALRSSAAFNKLLPEV
                     QLDAAVSRNFSKSFDIFENRIQNKALSKIVVLAVGVNSLDNYKTDLSQFIKSLPKGHR
                     LIIVTPYNAKNMSQVTTVRDYELSLMKKYNYITVADWYKVATEHPEIWGNTDGVHYSD
                     SDTTGADLYVSNVKKAIQKSAQRAAK"
     gene            complement(23685..24065)
                     /locus_tag="VBD27_000346"
     CDS             complement(23685..24065)
                     /locus_tag="VBD27_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024383.1"
                     /translation="MKNFKITRTHLEILSLIIIVVFGLSVFTLTTSSQGVLSYDGGKI
                     KYVGSIVNHHMTGKGKLTYENGDYYKGDFVNGVFEGKGTFVSVHGWSYTGDFKKGQPD
                     GQGRLNAKNKKVYKGTFKQGIYQK"
     gene            complement(24072..24509)
                     /locus_tag="VBD27_000347"
     CDS             complement(24072..24509)
                     /locus_tag="VBD27_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEO7024384.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMANLDKEDSLSVESRATSTWEHG
                     NPIHRGTQAILQRYQIPYTNDKGSQPISKDDLAYFDYIIGMDEQNVKDLKALSQGTYD
                     DKISLFCDGGVPDPWYTGDFEETYRLVSDGCKQWLTLINNSRD"
     gene            complement(24661..25659)
                     /gene="ruvB"
                     /locus_tag="VBD27_000348"
     CDS             complement(24661..25659)
                     /gene="ruvB"
                     /locus_tag="VBD27_000348"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO7024385.1"
                     /translation="MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEA
                     AKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAIEKSGDLVAILNDLE
                     PGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPR
                     IANRLLKRVRDYAQIMGDGLIDDNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGG
                     PVGLGTLSVNITEERDTVEDMYEPYLIQKGFIMRTRTGRVATDKAYEHLGYQRFDK"
BASE COUNT         7746 a         5338 c         4414 g         8261 t
ORIGIN      
        1 tttagtatag acataactat tcctctatat tcaattaaga gaaattatat aaccactatt
       61 gagaaatgta gtctagctat taaactcttt gctcatcgaa aatatccaat aaatataata
      121 cataaaacgc ctgcttacga aatataaaca aaattgtttg catttcgtaa acaagcgtta
      181 cctatttaac aatatatgat gagtgttccc gctgagaata attctcagcg gtagaccaga
      241 gctagactaa gaatcgattg attccatcat cataacactc aacaaaattg ataaaaatta
      301 tactaattca ataattgcca ttggggcagc atcgccacgg cgtggttctg ttttaagaat
      361 acgagtgtat cctccgttac gttcagcata acgaggtgcg atgtcgtcaa aaagtttttg
      421 aagagctgtt gttgatgtat atttatcaga agcttcatca tagttttctg atgcaatttc
      481 attacgtaca taagcagcag cttgacgacg agcatgtaaa tcaccacgtt tacctagagt
      541 aatcattttt tcaactgttt tacggatttc tttagcacgt gcttcagtag ttacaattga
      601 ttcgttgata agaagatcgg ttgtcaaatc acgaagcatt gccttacgtt gtgagctagt
      661 gcgtcctagt ttacggtaag ccattatgtc ctcctatttt atttatcgtt ttttaatcca
      721 agacctagat cggcaagttt gattttaact tcttcaagac tcttacgtcc taagtttcgg
      781 actttcatca tttcaggctc agttttttct gttaaatcaa atactgtatt aattccagca
      841 cgttttaaac agttatatga gcgcactgac aaatcaagtt cctcaattgt gcggtcaagc
      901 actttctcat cgttcacttt ctctgtttcc ttcattactt cagttgcttt agcaacctct
      961 gttaaatcag taaacaagtt taagtgttca attaagacgc gagctgaaag accaagagca
     1021 tcctcaggaa tgattgtgcc atttgtcatg atttcaatag ttaatttatc aaagccatca
     1081 ttactaccta cacgagcagg ttcaacttga taattaactt ttttcactgg tgtatagatt
     1141 gaatctacag ccaatgttcc tactggtgca tcatcttttt tatttccttc tgctggaaca
     1201 taacctcggt ttttagcaac ggtcattgtt gctttcaaac tgtgaccttc agcaattgtg
     1261 aaaagataat ggtcagggtt tacaatttcg atatcactat ctgttaaaat atctccggca
     1321 gtaatttctg caggaccttc aacgtctagt tcgataatct tttcgtcttc gacgtaagat
     1381 ttcacagcaa gtccttttac attaaggatg atttgcatca catcttcacg tacacctggg
     1441 atagtatcaa attcgtgtaa tactccatca attttgattg atgtgactgc tgcacctgga
     1501 agtgaagaca gaagcacacg acgaagagag tttcctagag ttgtaccgta gccacgttct
     1561 agtggttcga tgacaaatct gccgtaatct ttattttcat caatttttgt tattattggt
     1621 ttttcaaact caatcattta gttactccct cttaaacgaa aagttgtgta ctataaaatg
     1681 ctatgattat acacgacgac gttttggagg acgagcacca ttatgtggta caggagtcac
     1741 atcacgaatt gcagttactt caagaccagc tgcagcaagg gcacggatag cagattcacg
     1801 acctgaacca ggacctttta cagtaacttc aacagtttta agaccgtgtt cttgtgcaga
     1861 tttagcagca gcttcagcag ccatttgagc agcaaatgga gtagatttac gagaaccttt
     1921 aaatccaaga gcaccagctg atgaccatgc aagagcgtta ccatgcacat ctgtaatcat
     1981 aacaatagtg ttattgaatg tagcgtgaat atgagcaaca ccagattcga tgttcttttt
     2041 cacacgacgt ttgcgtgttg gtttagccaa ttgttttacc tcctatttta ttttttctta
     2101 cctgcaatcg caacagcttt acctttacga gtgcgagcgt tatttttagt attttgtcca
     2161 cggacaggaa gtccacgacg gtgacggatt ccacggtatg aaccgatttc catcaaacgt
     2221 ttaatattca agtttacctc acgacgtagg tcaccttcaa ctttgattgc atcaacttca
     2281 cgacggatag cgtcctcttg atctggtgtc aaatctttaa cgcggatatc ttcagaaata
     2341 cctgcagctg ctaagatttt tttagatgta gaaagaccaa ttccgtatac ataagtaagt
     2401 gaaattacta cacgtttgtc atttggaata tcaactccag caatacgagc cattttatct
     2461 cctttctata ttatccttga cgttgtttgt gttttggatt tgtcggacaa attaccataa
     2521 cacgaccgtt acgacgaata actttacagt attcgcaaat tggtttaacc gatggtctta
     2581 ccttcatgtt ttaatccctc caattatttc gactatttaa agcggtatgt gatgcgtcca
     2641 cgtgttaaat cgtatgggct catttcaaca gtaacacgat cacctaccaa aatacggatg
     2701 tagtttttac ggatttttcc tgatacagtt gccaaaattt ggtgtccatt ttccaactca
     2761 acagtaaaca ttgcgttagg catagtttca acaactttac cttcaatttc aatcacatct
     2821 tcttttgcca cgcaaaagta ccccctaaat ttcgatttga tgtcctctat aaagaggtaa
     2881 caattataag tcagactaac ttagtatatc attactaatc tacttatgca agtagattag
     2941 cataatttat ttgagttcta gcaacgcttt ttcaacatct gcaaaaactt ctgttatttc
     3001 ttgattacct tcaatatctg taacaagacc aagcttacga tagtgttcaa gaataggttc
     3061 tccttgagca atattaacgt ccaagcgacg tttgacagtt tcaggcttat catcttcacg
     3121 ttgatagtaa tcttcttctt tataatctac tggtgggttg aacactttgt ggaaagtttc
     3181 accagtttta cgattgataa tacggccact caaacgctct ataaggcatg ttggatccac
     3241 tttaatatta ataacaccat ctaagcgtag tcctagttct tcaagcgtag catctaaggc
     3301 gtgtgcttgc tcaatagtac gtggatatcc atcaagtaaa aaaccttttt ctgcgatatc
     3361 atcctcagct aagcgctctt ttacaatccc gtttgttact tcatcaggaa ccaattcacc
     3421 tttatcaata taacttttag ctaaacgtcc catttgggtt tgattagcca ttgcggcgcg
     3481 gaacatatcc cctgttgaga tgtgagcaac accaaattct tcaacaatct tagctgcttg
     3541 agtaccttta ccagcaccag gcaaacccat aattaaaaga ttcattatag tctccttatt
     3601 ttgtttttaa ataaatccaa cacagcatgt cggacttatt aaaaaacaaa atagaaggtt
     3661 gacagagtca acctatattc tattctgttg tattcatgaa tccaacatag cgtctcttca
     3721 aaagatagcc ctcaagttgt ttcatacctt caatacctgt tgcaatcaaa atgagtaagc
     3781 ttgtaccacc taacgcaata cttgatgaca aatgcaacgc ttgttgagca ataattggta
     3841 acaaggaaat aaatgctaga aatactgaac caattgttgc taattttttc aatagcgatg
     3901 acatatactc ttctgtttct cgtcctggtc gaatacttgg aatgtaggac gaattttttt
     3961 gaagattctc tgctgtcttt tcagggttta cttgtacaaa agtatagaag aaagagaaca
     4021 gaataatcaa gatagcatag ataatcatac cgactggggt ttgataatta agtaattctt
     4081 ggagtttagt aagccaagga atttctttac cattttgaaa gaaaggaata atcgtacttg
     4141 gaattgttgt aatcgagcta gcaaagataa cgggaataac accagctgga tttactttta
     4201 gaggaagata tgaacttgta ggagctcctt gaacaagttt tgtatattgg attggaattt
     4261 tgtattcagc ttgttgaata aatgttgtaa aaaatacaat tgctaatact gctacaataa
     4321 gaatcccgac aaagatgtaa gaatttgtaa ttgctgaaga gcgtacattt acaaagaaat
     4381 cttcgtagat agttgtaatt gcacttggaa tggaagaaat gatccccgca aagataatca
     4441 tagacactcc atttccgaat cccttatcag taatttgttc acctagccaa gtaaccacca
     4501 tactacctgt tgtaaggatt gcaccaataa gcaaatatgt ttgtacattc ggagttttaa
     4561 ctaaagcaac acttgataga gtgttaaaac cagcagtaat accaattgat tgtacgaatg
     4621 caaggaacaa agaaatataa cgtgtagctt ggttaagctt acgacgacct acctctcctt
     4681 gttttcccca ttcaacaaac ttcggaagaa tatccatttg aagtagctga acaacaatag
     4741 acgcagtaat gtatgggcta actcccatag agaataccga aaagtttctc attgcattac
     4801 cactaactaa gttaagcata tttagaaatg gaagttcacc catttgttct aaactcttaa
     4861 cattgatgcc cggtactgtg atatgcgttc cgatacgaaa tacaagaagg ataaagatag
     4921 taaataaaat tttatttctt accatcttta ctttgagtgc atctctaagt aattttaaaa
     4981 acataatgag ttacctctca ttaattagat gacttcgatt gaaccacctt tagcagtgat
     5041 agctgcttca gcagatttag aaaatttagc tgctttaaca gacaatttct tggttaattc
     5101 accattacca agaattttaa cgcctgattt ttcagcacgc acgattccag cttctttaag
     5161 aacaactgga gttacttcag taccatcttc aaagacattt aattggtcaa ggttcacaag
     5221 agcatattct tttgcattga tgtttgagaa cccacgtttt ggcatacgac ggaacaatgg
     5281 agtttgtcca ccttcaaaac caaggcgtac tccaccacca ctacgcgctt tttgtccttt
     5341 ttgaccacgt ccagaagttt taccattacc tgaagatgta ccgcgaccca cacgattgcg
     5401 gactttacgt gatccttctg caggttttaa ttcatgaagt ttcattattt tttctcctta
     5461 tttgtaaaat gctaacgcct ttacgagggg aaaggtactc cccgcaaaga ctcgcctata
     5521 cgatttgttt aaatcgcacc aataacaatc agtgcgattt agcttttaat taagcttctt
     5581 caacagttac taagtgagag atagcgttaa ccataccacg gatggcagca ttatcttcct
     5641 taacaactga agagtttaat ttaccaagtc caagggcaac aactgtttta cgttgttctg
     5701 gtttgcgacc gattggagac ttagtcaaag taattttaat ttgagccatt ataatcccct
     5761 ttcttatgct aagtcagaaa cagaaatgcc acgtaaagca gcaacttctt ctgcacgttt
     5821 taattgtttc aagccctcaa cagttgcgcg aacaatgttg attggagtgt ttgaaccaag
     5881 tgatttagat gtaatatctg caacacctgc taattcgatg acggcacgaa cagcaccacc
     5941 agcggcaacc ccagcaccct caacagctgg tttcaacaat actttcgctc caccaaattc
     6001 tgaacgaact tcgtgaggaa ttgttgtacc gaccatagga acttcaacca tgtttttctt
     6061 agcagcttca acagctttgc gaatagcttc tggtacttct tgagctttac ctgtaccaaa
     6121 accaacacga ccattacggt caccaacaac tacaagagct gcaaaacgta gacgacgtcc
     6181 acctttaaca acttttgtaa cgcggttaat cgcaacaacg cgctcttcaa gttctactgc
     6241 attatcttta aatgccatta ttaagtgtcc tccctattag aatttcaatc cgttttcacg
     6301 agctgagtca gccaaagctt ttacacgtcc gtgatagaga tatccaccgc ggtcaaacac
     6361 cacttcagaa atacctttag ctactgcgcg ttcagcaaca agtttaccga caacaacggc
     6421 ttgttctgtt tttgttccgt tagaaacttc tttatcaaga gttgatgcac ttgcgagcgt
     6481 tacacccgct acgtcatcaa ttacttgagc gtagatgcct gtattagaac ggaaaatgtt
     6541 caaacgtggg cgatcagcag ttccagagag tttaccgcga acgcgacggt ggcgtttttg
     6601 gcggatttta tttttatctg gtttcgaaat cacaattttc acctcttaaa tatattgtta
     6661 tgtcaaaatg acaatattaa aatgaaaaga tagagaattt gcagaaaaga ttctacaaaa
     6721 tctatcaatc ctattattta cctgttttac cttctttacg gcgtacgaat tcaccaacgt
     6781 aacgaatacc tttaccttta tatggttctg gtgaacgtaa gctacgaacg taagcagcag
     6841 tttgtccaac gctttctttg ttgataccga ttacgttgat tgttgttgga gttggcactt
     6901 caaaagtaac accttctgga gcttcaactt cgtcttggtg agacttacca acagaaagaa
     6961 caagttttga tccttgaagt tgtgcacggt aaccaacacc gcgcatttca agagcttttt
     7021 tgaaaccttc agaaacacca acgaccatgt tgttcaagtt cgcacgagtt gtaccatgaa
     7081 ttgttttcat ttcttttgag tcgttaggac gagtaactgt tacttcagtc ccttcaacag
     7141 tgattccaat atttttatta aactcacgag tgagttcgcc tttaggtcct tttacagtaa
     7201 caacattatc cttgttaatg atttcaacac ctgcaggcaa tgtaattact ttattaccaa
     7261 tacgtgacat gtttattttc tcctgttaaa ttgtcaagct gcttgagcaa ctagttttca
     7321 cggggactca tgagaccaca ggattatact gtgactcaat gagcatttta ggtaaaattt
     7381 taccaaatat atgcaagtac ttctccacca acattttttt ggcgagcttc tttgtcagtc
     7441 aaaagacctt cagaagttga tacaattgca ataccaagtc cgttaagaac tttaggcata
     7501 tcttcatgtt tagtataaac acgaagacct ggttttgaaa tacgtttcaa gttagtgata
     7561 acacgttcac cattttgtcc atatttaagg aacacacgga tgatgccttg tttgtcatct
     7621 tcaataactt caacgttttt tacaaaacct tcacgtttaa ggatatcagc aatccctttt
     7681 ttaatgtttg atgcaggtac ttctaacact tcatgttttg cttggttagc attacgaata
     7741 cgagttaaaa agtctgcaat tgggtcagtc ataaccattt tttaattctc ctcttattag
     7801 cagtttgaat ttaatcactt gctaattaat gagtccttac ggacgttgta agactaggtc
     7861 ttagtatatt tgcgtttaaa aatcagtgcc attatcaatg acacctttta aacacaaagc
     7921 atcattaatt tattaccaag atgctttagt tacgcctggt acttgacctt tataagccaa
     7981 gtcacggaag caaacacggc aaagttggaa tttgcggtaa actgagtgtg gacgtccgca
     8041 tttttcacaa cgagtgtaag cttgcgtaga gaacttcgct gggcgtttgt tcttagcaat
     8101 catagatttt ttagccaatt tatttacctc ctagattact ttgcaaaagg cattccaagg
     8161 ccttttagca attcacgtga ctcttcatca gtgttagcag tagtaacgat aacgatatca
     8221 agaccacgta ctttatcaac gtcatcaaag ttgatttctg ggaagatcaa ttgttcttta
     8281 actccaagag tgtagtttcc acgtccatca aatgatttcg ttggaacacc atggaagtca
     8341 cgtacacgtg gaagtgaaac agaaactaat ttatccaaga attcgtacat acgttcacca
     8401 cggagggtaa cttttgcacc gatcgctaca ccttcacgaa gacggaagcc agcgattgat
     8461 ttcttagctt tagtaataag tggtttttga cctgaaataa gtgctaactc agcagcagct
     8521 ttctcaagat ttttagcgtt tgatacagca tcaccaacac ccatgttaag aacgattttc
     8581 tcaacttttg gcacagccat aacagatgaa tagttaaatt tttctgtcaa cgcaggaaca
     8641 acttcattag tatatttttc ttttaagcga tttgccatta tgcttctcct ttccttcgtg
     8701 attaatcaag cacttcgcct gattttttgt tgtaacgaac ttttttgccg tcaacaactt
     8761 tgtaaccaac acgtcctgct acaccatttt tatcaagaac ttgaacgttt gacacatgga
     8821 ttggagcttc tttttcaacg atagcgcctt gagggttttc gttatttggt ttttgatgtt
     8881 tcttgatgag ggctacgcct tcaacaacaa ctttgtttac ttttggaaga gctttaagaa
     8941 ctacagcttc agtgcctttg tccttaccag caataacgcg aactttgtcg ccttttttta
     9001 caaacatttg agtttttctc ctatttttct tacgcctcga aaggcaccct aggaaatcct
     9061 aggggactac gtttgttaat ctgagattaa agtacttctg gtgccaatga aacgatcttc
     9121 atgtagccac cttcacgcaa ttcacgtgca actgggccaa agatacgagt tccgcgagga
     9181 gttttatcgt cacggatgat tacagcagca ttgtcgtcaa atttgatgta tgaaccgtct
     9241 gggcggcgtg caccagtttt tgtacgaacg ataacagctt taaccacatc acctttttta
     9301 actgctccac caggagtagc ttgttttaca gaagcaacga ttacgtcacc gatgttagcg
     9361 aatttacgtc ctgaaccacc aagtactttg atagtcaaga tctcacgagc accgctatta
     9421 tcagcaactt tcaagcgagt ttcttgttga atcatttcaa ttttctcctt ttagtttgat
     9481 tagataataa cagctttttc cactacttcc acaagacgga aacgttttgt agctgaaagt
     9541 gggcgagttt ccatgatacg aacgatatcg ccttctttag caacgttgtt ttcatcatgt
     9601 gctttatatt ttttagaata gttgatacgt ttaccataga ctgggtggtt acgtttagtt
     9661 tcaactacaa ctgtgattgt tttgtccatc ttgtcagaca ctacgcgtcc gtaaagagtt
     9721 ttacgttgat tacgttccat ttagaatttc tcctttccca atctattatt tcatttctga
     9781 ttgcacagtc ttaacacgtg caatttgttt cttaacttcg tcaaggcgag cagtcttttc
     9841 aagttgacct gctgcagctt ggaaacgaag atcaaacaat tcttttttga gttcgttttc
     9901 tttcttagca agttcttctt gagacaatcc acgaagctct ttaacaaaat ctttaatttc
     9961 ttgaagtttc atgtcttctc cttattctgc ttcacgtttt acgaatttgc atttaactgg
    10021 taatttgtgg ctagcaagac gtaatgcttc acgtgcaact tcttcagaaa caccagcaat
    10081 ttcaaacatc actttaccac gtttaactgg tgctacccaa ccttcaggtg tccctttacc
    10141 agaacccata cgtacaccga tagcttttgc agtgtatgat ttgtgtggga agattttaat
    10201 ccaaacttta ccaccacgtt tcatgtaacg agtcatcgca atacgggcag cttcgatttg
    10261 acggtttgta atccagtggc tagttgtagc ttgaagtccg tattcaccga atgaaacttc
    10321 tttaccacct ttagcctcac cgcgcatttt tccacggaat tcgcgacggt gtttaacacg
    10381 tttaggtact aacatttgtt atttgcctcc tttagtgttt ttacgagctg gaagaacttc
    10441 tccacggtaa atccaaactt taacgccaag tttaccataa gttgtgtcag cttcttccca
    10501 agcatagtcg atatcagcac gaagcgtgtg aagtggaaca gttccttctg aataaccttc
    10561 agcacgagcg atatcagcac cgttcaaacg accagaaact tgagttttaa tacctttagc
    10621 tcctgcacgc attgtacgtt ggattgcttg tttttgagca cgacggaaag caacacgttg
    10681 ctctaattga cgagcaatat tttcaccaac aaggtgagca tctaaatcag gttgtttgat
    10741 ttcaatgatg ttgatgtgta cttgttttcc agttaatttg ttaagttgac cacgaagagc
    10801 gtcaacgttt gctccacctt taccgataac catacctggt ttagcagtgt gaagtgaaac
    10861 aataacttta tttactgcac gttcaatttc aatagttgaa actgatgcgt cagctaattc
    10921 tttattaatg aatttacgga ttgcaagatc ttcatgaagg taatccgcgt attctttttc
    10981 agcataccat ttcgcatccc agtcacggat gattccgaca cgcataccaa ttggatgtac
    11041 tttttgaccc acgattttac ctccttattt ttctgataca actacagtta cgtgagttgt
    11101 acgtttgttg attggtgaag ctgaaccttt cgcacgtgga cggaaacgtt tcattgttgg
    11161 tccttcgtta gcgaaagttt cagatactac caagtttgct ttttccaaac caaagttgtt
    11221 ttctgcattt gcaattgctg agttaagagt tttctcaata acacgagctg ctttgtttgg
    11281 agtgaatttt aagattgcga ttgcgtcagc aacattctta ccacggataa gatcaagtac
    11341 taaacgtgtt ttacgaggtg aaacacgcac tgtacgagcc attgctttag ctgaagtaat
    11401 ttctgccatt gtgtcctcct cctattaacg acgtgttttc ttgtcgtcag ctgcgtgacc
    11461 tttgtaagta cgtgttggtg caaattcacc aagtttgtga cctaccatgt cctcttggat
    11521 ataaacagga acatgtttac gtccatcata aactgcgatt gtgtatccga tgaaacttgg
    11581 gaaaatcgtt gaacgacgtg accaagtttt gattactttt ttcttttcgt catttgcttg
    11641 agcttcaact tttttcatta aatgctcgtc gacgaaaggt ccttttttaa gactacgtcc
    11701 cattttgtag ttttctcctt taaataatgt accacaacgg cctgtacact atagtgtact
    11761 accgagctgg cggatttgta taagattatg ctattaacct attatttttg gttacgacga
    11821 cgaacaataa gtttgtctga tttagctttc ttattacgag ttttaagtcc aagcgcaggt
    11881 ttaccccatg gagtagatgg tgctttacga ccaactggtg ctttaccttc accaccaccg
    11941 tgtgggtgat cgttagggtt cattacagaa ccacgaactg tagggcgaac acctttccaa
    12001 cgattacgtc ctgctttacc aatgttaaca agtgattgct gttcattacc aacagtacca
    12061 attgttgcac tgcaagtacc aaggatcata cgtacttcac ctgattgaag gcgaacaaga
    12121 acatatttac cttcttgacc aagtacttga gcagaagcac cagcggcacg aatcaattca
    12181 gcacctttac ctggttgcaa ttcaatatta tggataactg taccgactgg aatgttagca
    12241 agcggaagtg cgttaccaac tttgatgtct gcttctggac cagaaataat gcgttgacct
    12301 acttcaaggc ctttaggcgc aaggatgtaa gctttaacac catcagtata gtgtacaaga
    12361 gcaatatttg cagtacgatt tggatcgtat tcaattgttt taacaactgc ttcaacacca
    12421 tctttattac gtttgaagtc aatcaaacgg taatgacgtt tgtgtccacc accttggtga
    12481 cgaacagtaa tacgtccgtt gttgttacgt cctgctttat tctttagtga aacaagcaat
    12541 gatttctcag gagtgtttgt agtgatttct gcaaaatcca aagaagtcat attacgacgg
    12601 ccatttgtcg ttggtttata aactttaata cccacgttat ttcctcctta gattattcag
    12661 cttcagcagc aaacaactcg attgcttttg aatcagctgt aagagtgata atagcttttt
    12721 tagtttttga agtaaaacct gtgtaacgac caacacgttt tgctttgggt ttaactgtca
    12781 cagtgtttac acttgcaact tttactccgt caaatgcagc ttcaacagct tgtttgatca
    12841 aaagtttgtg tgcacgtgtg tcaacttcaa atgtatattt ccctgcttca agagcaacca
    12901 ttgatttctc agtgataaca ggttttttga ttacgtcata caaattcatt atgcaagaac
    12961 cccctcgatt gtagagattg cttctttagt aacaagaagt ttgtctgcgt taacgatatc
    13021 aagaacactt gcagtagttg cagtagcaac tgtaacgttt ggaaggttac gcgctgaaag
    13081 tgcagcaaat tcgttacctt cttcaaggat tacaagtact tttgaatcaa tgctaagtgc
    13141 tgaaagaact gacgcaaatt cagcagtttt tggagctgca aatgaaaggt tttctacagc
    13201 tacaaattta tcttcagcaa cttttgctga ataaactgat ttcaacgcaa ggcgacgaac
    13261 tttttgtgga agtttgtagc cgtatgaacg tggagttggt ccaaagacaa caccaccacc
    13321 acgccattgt ggtgagcgga tagaaccttg acgagcgcgt ccagttcctt tttgacgcca
    13381 tggtttacgt ccaccaccag atactgctga acggttttta accgcgtgag taccttggcg
    13441 aaggctagcg cgttggctga ttacaacatc aaaaacaact gattcgtttg gttcgatacc
    13501 gaagatagct tcgttaagct caactgagct aacttcttta ccagtttggt caaatagttt
    13561 aacgtttgcc attttaactg ttttctcctt tcttattatt tagcagcttt aactgctgat
    13621 ttgatagtga taagagattt cttagcacct ggtacgttac ctttgatgag gacaacgttt
    13681 ttctctggga taacttgtac aatttcaagg ttttgaactg ttacacggtt accacccata
    13741 cgtcctgcca agcgtttgtt tttgaaaacg cggttaggcg caacaggtcc catagaacct
    13801 ggacggcgat ggtaacgaga accgtgagcc ataggaccac gtgattgacc atggcgtttg
    13861 ataacacctt ggaaaccttt accttttgat gtccctgtga cgtcaacaac atcaccagct
    13921 tcaaattgtt ctacagataa ttctgcacca acttctaagc cttcaatgtt tttgaattca
    13981 cgaatgaagc gcttaggagc tgtgtttgct tttgcaacat ggcctttggc aggtttgtta
    14041 ctcaatactt cacgtttgtc atcaaaacca acctgaactg cttcgtaacc gtctgtttca
    14101 acagttttaa cttgaagcac aacgtttgga gttgcttcaa tgacagtaac agggatgaat
    14161 tcgcctgatt cagtgaagat ttgagtcatt cccacttttt tccctaagat tccttttgtc
    14221 atgagaaaat atttcctttt cttattttat ttcaaaaagt tttttacgag cgttttttgt
    14281 gctcagaaat caaattaaag tttgatttct acgttgacac cacttggaag atcaagtttc
    14341 atcaaagcat caacagtttt ttgtgttggg ttgatgatgt ctaccaaacg tttgtgtgta
    14401 cgcatttcaa actgttcgcg agaatcttta tatttgtgag tcgcacgaat aattgtgtaa
    14461 agactgcgtt cagttggaag cggaactggt ccagcaactg tagcacctgt acgtgttgca
    14521 gtttcaacga ttttttctgc cgctgtatca agtgtacggt gttcgtacgc tttcaaacgg
    14581 atacggattt ttttgtttgc catcttttct cctctcgtct atttacgata ataggctagc
    14641 tcctcaagaa aaccaacacg agttgcgtgg caatgcaacc tagcgtgtcg caacctcttg
    14701 catcatagct aaagctgttc atacacagca ccagtatatg atatcaaaaa aaaacagcac
    14761 acgcaagcat tattttattt tttttcaaaa aaaatgcaac catttctggt tacatttttt
    14821 gaatatatcg atgatagcga actttcaaaa acagccacct aaagatatta tctgctatcg
    14881 tagccatcca aaccccaagc aaacctaatt caaaaactat tcctaataaa taaccaagaa
    14941 ctactctgat taaccacatg cctatagtcg ttgtgtaaaa tgggagttta gcatttccta
    15001 atccttgcca agctgctgta taaaccaagg tcccaatagt agcaggtact cctacgaaag
    15061 atagcaaaat aacaatcaaa cttcctttaa tggcagaagg attatttgta aataactgtg
    15121 tcaatggttt gccggcaaga agcataaaac ttgatataag gatcattaga actaagccaa
    15181 tataatacga actttgaata gtttcttcta tagattctct atttttttgt cctacatatt
    15241 ttgcagttag gataattgta gcagttgcga tgccaagtcc tggcatataa ttaaattgtg
    15301 tcaaggtttc tccaatagca tttcccgcta caacatttgt acctaactga acaacaatag
    15361 caactataac aacatctcca gctcgcatca tcagacgttc tcctgctgat ggtaaggcaa
    15421 gattccaaat ttgtgcactg attttccaag tcattctttt tattattttt ttaataggaa
    15481 gatacttcgc aaggatacaa ataccaatta atcttgcgat aagagttgat actgccactc
    15541 cgaccaaacc atagcgccac tcaaaaatag caaaacctga taagactgca ttgaggaaat
    15601 tgacaaaaat actgacatac attggtaaac gaggttgccc ttgcacacgt aaaaatgagc
    15661 ctagggtggt taatattcct aaagtaacaa cacctccacc cacaattgct aaatataaac
    15721 caccaacttg tgcgacactt tttgttgtcc ctaaaagttt aaagaaagtt tgtccaaaaa
    15781 caatcgagat aattcctaat actgccccta tcagtgatgt tagaaaaatg gcttgagaac
    15841 atactgaaat tgcatcatcc ttctcactac ctgctaacga cttggccaat agacttgcta
    15901 tactcgcccc taaagctata aaaatagctt gataaatagt aattatatta ttagcaactg
    15961 aaacacctga tactgctaca acacctaact gagccactag ataattatca actactccca
    16021 tgagcatttg taagatattt tcaatcatag ctggtatagc taactgaata atctctttac
    16081 tataacgcac agtatctcct ttctattacc caggatcatt aaaaagagag tgatgggaaa
    16141 atttctaaaa tgtttctttc tcgctctaaa tattttaaaa caatcgtatt tttattattg
    16201 tttcaaacct agacactctt caaccgtttc tcgcatattt gaggtaggta ctaccattct
    16261 atggattact tcggcctttt gtagatctgt aaccgcctta ggtaaagaaa cacctgacaa
    16321 atcgtttaag attgaaatag cagcgaaatc ctgtatctct tctttatttg taatagcctt
    16381 tgtaacaaca cacgggaact tataaggact tgctgttgac acaattactg ttggagtttg
    16441 atctttagtt tctgtctgat aagcttgata aacagctgat gctacagctg tatgcggatc
    16501 ctctatatac tgatactggt caaataaacg tttgatgtct aattcaacaa attgttctgt
    16561 cgcaaatccc gctacaaagc tctctaaaat atttgcttgc ctagcctcta aggcatactc
    16621 cccagtagtc actaaatctt ccattaattt tttagtcgtt tctgcatcat cgcctaaaag
    16681 gtgaaagatt aatcgttcta gattagagga gaccaaaata tccatagagg gacttgatgt
    16741 cactttaaat tctcgatttt tatcgtaggt ttgtgtctta aaaaaatctg ttagaacgtt
    16801 attatcattt gaggcacaaa tgagtttggt aacaggtaat ccaatttgac tagcataata
    16861 tgctgctaaa atattaccaa aatttcctgt aggtacacta aaattaatta gttgaccaat
    16921 tgagatttcc tttgacttaa ctaattgagc atatgcatat atatagtaaa caatttgagg
    16981 aacaaggcga ccgatgttca ttgaattggc tgatgataac tgcatatgct gctgtgataa
    17041 ttttgatcga agaagggaat tattgaacat ttctttaacc gaagtctgtg catcatcaaa
    17101 atttccttca atcgcaacaa catgggtatt ttgcccggct tgtgttatca tctgcaattc
    17161 ttgaatataa ctaacaccat gtttaggata aaatacaata atctcagttc ctggaacatc
    17221 tgcaaaacct gccatcgctg ccttaccagt gtcacctgat gttgctgtta aaataacgat
    17281 tttatcatca accccttgtt ttttggctgc tgttgttagc aaataaggaa gaatggataa
    17341 agccatatct ttaaatgcaa ttgtaggacc gtgaaataat tcaaggtgat accggtctcc
    17401 aatctttact attggggcta tttcagttgt atcaaattta gtatcataag cttgactaat
    17461 acagtaatcc aattcttgtt cagtaaaatc atcaaaaaaa gctgacaaaa cgagtttagc
    17521 cacttcttgg taggaagcat ccttaagttt agtgaaatct aaatcaactt ttggaaatgt
    17581 gatgggggta aagagtcctc catcgtcagc taatcccttt agaatcgctt gactagctgt
    17641 cactttatta ctagcatcac gtgttgattg atatactatt gccataagaa tccctcttta
    17701 tataaatatc ttttagttat tgtaacacaa tttaataaca tgtccattgt tcgttatata
    17761 attaaccccc ctttattaaa ataaaggggg gcgtaatatc attatcactt tgtaaagtcg
    17821 agaactttac gtccttgaat caatcctctt tccatttcat cgaatacttg aggagctgta
    17881 tccacaggca ctttttcaac cactggtact accaaacctt ctgctccaaa tgcgaaggct
    17941 tcttctaaat ctttacgagt tccaactaag gaaccaacca cacgaatccc atcaagtact
    18001 gtttttacaa tactcaattc catatattct gatggaagac caacagctac cactgtacct
    18061 ccagcacgaa cgctgtcaat cgcctgatta aacgcaactt tagaaacagc agtcacaacc
    18121 actccatgac aaccgcctgt cttttcttga atataggccg caacatcttt tatttctttg
    18181 ccattaactg tcaaatctgc tccaacctct ttagctaatt gaagtttatc tgcgttaata
    18241 tcaacagcta caacatgagc attgaatact ttttttgcat attggactgc taagtttcca
    18301 agaccacctg caccatacac tgcaatccac tgaccaggag cagctccagc ttctttgata
    18361 gccttgtatg ttgttactcc agcacaagtg attgatgatg cttgagctgg atctaatccc
    18421 tcaggaacct taaccgcata gtccgcggta acgatagcgt attcactcat accaccatca
    18481 acactgtatc cagcattttt aacactacga caaagcgtct cacgtcctgt agtacagtat
    18541 tcgcaatgac cgcacccttc aaagaaccat gcaatagaga cacgatcacc aaccctcaaa
    18601 gacgttacgc cttctccaat ttcttctact ataccaatgc cttcgtgacc caaaatgcgt
    18661 cctggtacct ggccgaaatc tccatgtgca acgtgtaaat cagtatggca cacaccacag
    18721 tattctactt ttacaagtgc ttcaccatga ccaacttttg gaagatcatg ctcaacaact
    18781 tcaacacctg tactagcttg attaacaaca accgctttca tgataaaacc tcctagaaat
    18841 aaatatatct tatttgtaat cgtttacatt ttatattgta ttattattca caaactatgt
    18901 caagtgattt tcagaaaatt ttttgaaaat attattaact ggttaatctt tttgaaacta
    18961 aaaatcccac tcaatagagt gagattcttc gactgttgtt acttacggcg tcctggacgt
    19021 tcctcataac cataataagc atcttcgatg atttcttgca tgtgatctac cattggaagg
    19081 cgtggatttg ctggtgaaca ttgatcttca tatgctaaga aagctagttc acgagatttt
    19141 tctttccatt ctttttcatc aatgccttgg tcacggaaat tcatcttaat acctaaacgt
    19201 gttccaaggt catacacagc ttttgcatat gattcgacag cttcttctgg tgttgctgct
    19261 ggtaagccga gtaatttagc gatatcttga tacttttcat cagcacgata gtaattgtac
    19321 ttaggccatg ttgctgtttt agctggacga gttccattat agcggataac atacggcaaa
    19381 agaattgcat ttgtacgtcc gtgtacggta tggaattgtg cgccaatttt atgcgccatt
    19441 gagtgagaaa tacctaagaa tgcattggcg aaagccatac ctgccatagt tgacgcattg
    19501 tgcatctttt cacgtgcttc aaaatcagca tcttttactg agcgttctaa gtagtcaaat
    19561 acaattttaa ttgcttgaag agcaagtccg tctgtataat catttgccat ctgtgaaaca
    19621 taagcctctg ttgcatgtgt caatacatcc atacccgtat cagccgcaat aaaaccagga
    19681 acagtcataa caagggccgg atcaacgata gctacagttg gtgttaatga ataatcagcg
    19741 attggatatt tacgattatt tgctttgtca gagataacgg caaatggtgt cacttcagaa
    19801 cctgttccag aagttgttgg aatagcaaca aattttgttt tcttaccaag ttctgggaat
    19861 ttgaacgcac gtttacggat atccatgaat ttttgaacga ggtcatggaa atcaacttct
    19921 ggttgttcat agaagagcca cataactttc gcagcatcca taggagaacc accacctaat
    19981 gcaataatag tgtctggttt gaaggtacgc attaaatcag tccctttcat tactgttgta
    20041 atgtctggat ctggttctac ttcagcaaaa atctgataaa caactttatt gcgacgaaga
    20101 tcaagttgtt caatgatacg atccaagaaa ccaagctcaa ccatggcgtg atctgtaaca
    20161 atcatgacac gttcaacatc gcgacatttt tgcaaatatt gaatagaatc acgttcgaag
    20221 tatgtttttg aagggacttt aaaccattgc atattatttc tacgtcttcc tacttttttg
    20281 atattcaaga gatttatagc actaacgtta tcaccaactg agttacgtcc atacgaacca
    20341 caaccaagag tcaatgatgg caagaaagca ttgtaaacat ccccaatacc accaaaagta
    20401 gaaggagaat tccaaataac acggatagca cgaattctag ttccaaattc acgagctaag
    20461 tctgcatctt ttgtatgaat agcagctgag tgccctagac cattaaactc aaccatttga
    20521 cgagcttttt caacaccatc ttctgtagat tcagctttca atactgcaat aacaggtgat
    20581 agtttttcac gtgtcaaagg ttctttttca ctaacttcag tacattctgc ggcaagaata
    20641 tttgttcctt ctggaacagt aaatccagct tgttcagcaa tccaaactgc tgattttcca
    20701 acaatatttg gatttaattt tgcaccagca cagtttttgc tatttgcttt tgcaccaaaa
    20761 cagaattctt caagaagagc tttttctttc ttattaacga aataggtatg ataagattta
    20821 aattcttcaa caaattcttt ataaatttct ttatcaataa taacagcctg ttctgacgca
    20881 cataccatac cgttatcaaa tgatttagac ataacaatat cgtgagcagc ttgacggata
    20941 ttagctgatt tttcaacata agctggaaca ttcccagcac ctacaccaag ggcaggtttc
    21001 ccacatgagt aggcagcttt aaccatcgca ttaccacctg tcgcaaggat agtggcaata
    21061 ccatcatgtt tcatcaaagc attagttgca tcaatcgatg gttgttcaat ccattggaca
    21121 caattttcag gagctccagc agcgatagca gcatcacgaa caatacgtgc tgcatgtgct
    21181 gatgattctt gtgctgatgg gtggaaggca aaaataattg gattacgtgt tttaagagag
    21241 attaatgact tgaaaatagc agttgaagtc gggttggttg ttggcgtaat accacagaca
    21301 actcccactg gttcagcaat taatgtcaag cctgtgacat cgtcttcttc aataactccc
    21361 actgtctttg tatgacgcat gttgttaacg acgtgttcac atgcaaataa attttttgtc
    21421 gctttatctt caaagacacc acgaccagtt tcttctactg catgaagagc aagttctcca
    21481 tgggcatcca aggctgcaac agaagcttta gctacaatat aatcaacttg ttcttgatta
    21541 agttgacgca tctcatcaag tgcttttaaa ccgttttgaa ctaatgtatc aattgcaaga
    21601 gcaacatctg tagtttctac cgctttagtt ttttcagtca tttgagactc cttttgattt
    21661 gttaaatgtt tcacaaattt gtttacgaga ttattatagc atatttaaaa actttgtaaa
    21721 tagtttcaca acttttttgt gaactttttt tcaatctttc ggaaacattt cttgaaagcg
    21781 ctttaattaa atttgttttt tcattactaa ttttcagatt aaagccaatc gatttcattt
    21841 gtatcaccat aaacggaggt atctattatt cgttttcaca aaaaagccga gtacatgact
    21901 cggacttgtt tattttgcag ctcgttgagc tgatttttga atagcttttt tgacgttact
    21961 aacatataaa tcagcccctg tagtatcgct atcactataa tgcacaccat ctgtatttcc
    22021 ccagatttct ggatgttctg tcgcaacctt ataccagtca gcaaccgtta tgtaattata
    22081 ttttttcatt agcgagagtt cataatcacg cactgtcgta acttgactca tatttttagc
    22141 attataaggc gttactataa tcaaacggtg tcccttaggc aaagatttaa taaattggct
    22201 caagtctgtc ttgtagttat ctaatgaatt cacaccaact gccaaaacaa caattttgga
    22261 caaggcttta ttttgaatac ggttttcaaa aatatcaaat gatttactaa aattacgact
    22321 caccgcagca tctaattgta cttctggtaa caatttatta aaagcagcac ttgaccttaa
    22381 ggcaactgaa tctccgatta ccgtcacatc actaagagca cctgcatccc ctgcggctag
    22441 tgtatgtgtt cggttagtat ttgttctagc ttgttgtaac gaattctgca ataattctgt
    22501 ctcaaaactt cctatacttg gagctgtcaa cattgtcact acagtaataa gagttagtac
    22561 ccctccgata ctaaataacc actttttgta aggcaacaag tcaaattcat aatcaaggaa
    22621 tttaggtttt ctacctaaaa taaaaggttc aatgatataa aatgataatg ttgagaagac
    22681 tgctgataat actactgtca aaataacagc tatccagttt ggggataaac gactaaaaat
    22741 aatataaaat ggccaatgaa aaaggtacat accgtaactg atatcagcaa gataagtaat
    22801 cacgaaaggt tctgaaatat ctggtgtatg ctcgtgtaaa atacgtgcat tgtaaatcat
    22861 tacactagca aaaatgctag acaaaacaaa accaaaaaga tatgttagcc gattatcaaa
    22921 atcaagtgca aaagttaaaa tcattagacc agcaaaagca ctacccatca tgattaaatt
    22981 atgcttcaaa gtcagatttt tgatattttt cttgaatcgt ccggtaatct ctcgaatacc
    23041 agatattgta gccaccatcg ctcctaaaaa gaacggaaat atatgtgata atgtagagaa
    23101 atatatcgtt gaaaaattat caacgaaaaa ggctctaaca aacatggtaa ggaagctgac
    23161 tccaaaaact cccatggaaa caaggaacaa agtccccctc aattgctttt gatcttttga
    23221 tcttttcgat aagagccata ccgttaagcc ccataaaaca taaaaatgga cttcaataga
    23281 aagactccaa gtatgcacaa ataagtgagg gataaattga ctttcgtagt taccaccagt
    23341 caagatttcg taaaagttgc tggtaaaacc taaagcagtc ataatttgag agccaatact
    23401 tgctctaaaa tcgcttttaa ccagaaatac aaaaggtatt gtcaccaaaa ccatcaatac
    23461 taatggtggg aaaatacggt aaaaacgtct acgacaaaaa ctaacaaaat caattttttt
    23521 cgttttagaa aattcatcaa tcaacaaggc cgtaatcaga aaaccagaaa atgtaaagaa
    23581 gatgtcaaca ccaacaaatc ctcctggaaa actattctta aagaaatggt aaagtaaaac
    23641 aagtagtagt cctgttatac gtaccaaaga aaaccatttt attctcattt ttgataaatc
    23701 ccctgtttaa atgtcccctt atagactttc ttatttttag cattgaggcg accttgtcca
    23761 tcaggttgac cttttttaaa gtcacctgta taagaccagc catgaactga aacaaatgtt
    23821 cctttccctt caaagacccc atttacaaaa tcgcccttat agtaatcccc attctcatag
    23881 gttaatttac ctttaccagt catatggtga ttaacaatag agcccacata ttttatctta
    23941 ccgccatcat agctaaggac tccttgacta gaagtcgtta aggtaaatac tgataaacca
    24001 aaaacaacaa taattatcaa agataatatt tctagatgag ttcgtgttat tttaaaattc
    24061 ttcatttcta cttaatctct actattattt attagcgtta accactgttt acagccatca
    24121 ctgactagac gataggtttc ctcaaaatca ccagtatacc atggatcagg aacaccacca
    24181 tcacaaaaaa ggctaatctt atcatcatag gttccttgtg atagagcttt taaatccttg
    24241 acattttgct catccatacc gatgatataa tcaaaatatg ctaaatcgtc tttagaaata
    24301 ggttgtgacc ctttatcgtt agtgtaagga atttgataac gctgcaaaat cgcttgcgtt
    24361 cccctatgta taggatttcc atgctcccat gttgaagtcg cgcgactctc aaccgagagg
    24421 ctatcctcct tatctaaatt cgccataaca aactcagcca tagggctacg gcagatattt
    24481 ccaagacaaa caaaacagac tttcttcata gcaaactcct tttggtctat tataacagaa
    24541 aaaaggtact tataatacgt ttaactgctc aaagaataac tacaacatat ctaatactta
    24601 acattgcatg aaaattaaaa aattaagact agatacccta gccttaaaat gacttatgtt
    24661 ttatttatca aatcgctgat aacctaaatg ttcgtatgcc ttatccgtag ctacacgacc
    24721 ggtacgggta cgcataataa aacctttttg aattaaataa ggttcgtaca tgtcttcaac
    24781 agtatctcgt tcttcagtaa tattaacgga tagagttcct aaaccaacag gacctccatt
    24841 atacatttca atcatggttc ttaagatttt ttgatcgacg taatcaagcc cctcgtgatc
    24901 aacatctaac atcgttaatg ctttatctgt aatattgtca tctatcaaac catctcccat
    24961 gatttgagca taatctcgaa cacgtttcaa taaacggtta gcgatacgtg gcgttccacg
    25021 actgcgacgc gctaattcag aagcagcttc ataagtaatt ttcatttcaa aaatatctgc
    25081 tgtacgctca ataatttctg tcaaatcgtt ttcttcataa tactccatat gccctgtaat
    25141 accaaagcga gcacgtaagg gattagataa catacctgca cgtgtcgttg caccaattaa
    25201 ggtaaagggc ggcaaatcta aatgaacact tctactagtt tctcctgcac cgatcataat
    25261 gtcaatatag aagtcttcca ttgcactata aagtacctct tcaaccgcca tcggcatacg
    25321 atgaatttca tcaataaaaa gaacatcacc tggttctaaa tcatttaaaa tggctactaa
    25381 atcccctgat ttttcaattg cgggacctga tgtttgtttg agattgacac ccaactcatt
    25441 agcaattaca aatgccatgg ttgttttccc taaaccagga gggccaaata ataacacatg
    25501 atccaatgac tcatcacgca atttagcagc ttcaataaat atttttagct gatctttaac
    25561 cttatcttgt ccaatatact ctcttaaata ctgcggacga agtgtacgtt ctaccaattc
    25621 ttcgtcaccc attgcatcac tatctaaaaa tcttgtcatg atattattat aacatttgta
    25681 taggaaaagt taagaactaa gttggtgtat tagtatctcc cttatttaaa ggaagaataa
    25741 actgatctta tagttaatc
//
LOCUS       DATRQA010000011        24697 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.011, whole genome shotgun sequence.
ACCESSION   DATRQA010000011 DATRQA010000000
VERSION     DATRQA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24697
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.011"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            56..1021
                     /locus_tag="VBD27_000349"
     CDS             56..1021
                     /locus_tag="VBD27_000349"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEO7024386.1"
                     /translation="MTIKNLTVAGSGVLGSQIAFQAAYKGMSVTIYDINDEALNKGKE
                     RIKKLAKVYQSEIETAKEAYSDKAKSIKYNKNLLPSLDHIFLSKVADSLDLIADLPNQ
                     ITFSKNLDQAVSDADLVIEAVPETVSIKEDFYKQLAKVAPSKTIFATNSSTLVPSQFA
                     DITGRPDKFLAMHFANNIWQNNIVEIMGHKGTDDEVVKEALAFSKDIGMVPLYIHKEQ
                     PGYILNSILVPFLESALALYYNKVSDSETIDKTWKLGTGAPMGPLEILDIIGIDTAYN
                     IMKNYSDTTSDPNSLHAHLAKMLKEEFIDKGRTGKAAGHGFYDYD"
     gene            complement(1068..1616)
                     /locus_tag="VBD27_000350"
     CDS             complement(1068..1616)
                     /locus_tag="VBD27_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO7024387.1"
                     /translation="MTKALISIDYTYDFVADDGKLTAGKPAQSIASAIADVTEKAYRS
                     GDYIFFAIDNHDIGDVFHPETNLFPEHNIKGTSGRNLYGPLGTLYETIKEDSRVFWID
                     KRHYSAFSGTDLDIRLRERRVDTLILTGVLTDICVLHTAIDAYNLGYKIEVPAAAVAS
                     LNDSNHQWALNHFKTVLGATIL"
     gene            complement(1683..2468)
                     /gene="codY"
                     /locus_tag="VBD27_000351"
     CDS             complement(1683..2468)
                     /gene="codY"
                     /locus_tag="VBD27_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEO7024388.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLDAELPYNTMAAQLADIIDCNAC
                     IINGGGNLLGYAMKYKTNTDRVEEFFETKQFPDYYVKSASRVYDTEANLSVDNDLSIF
                     PVETKENFQDGITTIAPIYGGGMRLGTFIIWRNDKEFSDDDLILVEIASTVVGIQLLN
                     LQTENLEENIRKQTAVTMAINTLSYSEMKAVAAILGELDGLEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFDKLKEYN"
     gene            complement(2594..3805)
                     /locus_tag="VBD27_000352"
     CDS             complement(2594..3805)
                     /locus_tag="VBD27_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7024389.1"
                     /translation="MKIFDKSMKLEHVAYDIRGPVLEEADRMRANGEKILRLNTGNPA
                     AFGFEAPDEVIRDLITNARESEGYSDSKGIFSARKAVMQYYQLQNIHVDMDDIYIVNG
                     VSEGISMSMQALLDNDDEVLVPMPDYPLWTACVSLAGGNAVHYICDEEANWYPDIDDI
                     KSKITSKTKAIVLINPNNPTGAVYPREILQEIVDIARQNDLIIFSDEVYDRLVMDGME
                     HIPIASIAEDIFTVTLSGLSKSHRICGFRVGWMVLSGPRQHVKGYIEGLNMLANMRLC
                     SNVLAQQVIQTSLGGQQSIDSMLLPGGRIYEQRNYIHKAINEIPGLSAVKPNAGLYLF
                     PKIDTDMYRIDNDEEFVLNFLKQEKVLLTHGRGFNMNTADHFRIVYLPRVDELTELQE
                     KMARFLSQYKR"
     gene            4072..4524
                     /locus_tag="VBD27_000353"
     CDS             4072..4524
                     /locus_tag="VBD27_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO7024390.1"
                     /translation="MSQKYERILVAIDGSTESELAFEKAVNVALRNDSELILTHVIDT
                     RALQSFATFDTYIYEKLEKEAKDVLEEYEKQAREKGADKVRQVIEFGNPKTLLAHDIP
                     EKEKVDLIMVGATGLNTFERFLIGSSSEYILRHAKVDLLIVRDPNKTM"
     gene            complement(4579..5961)
                     /locus_tag="VBD27_000354"
     CDS             complement(4579..5961)
                     /locus_tag="VBD27_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO7024391.1"
                     /translation="MAIKAVFFDIDGTLLNDRKNVQKSTIKAIRNLKDQGILVGLATG
                     RGPSFVQPFLENLGLDFAVTYNGQYIYSRSEIIYTNQLSKTTVYRLIRYAGARRREIS
                     LGTASGLLGSGIIGLGTSRLGQIVSSLVPRKWAKAIERSFKHFIRRIKPQNIDSLMVI
                     LREPIYQVVLVATEGESERIQKQFPRVKLTRSSPYSMDVISEGQSKVKGIERVGQCYG
                     FDLSEVIAFGDSDNDIEMLSQVGIGVAMGNASQQVKENARYTTADNNDDGISKALAHY
                     GLIQFEIEKTFSSRDENFNKVKSFHLLMDGETIKTPRLYDSKEAGFRSDFKVEEIVEF
                     LYAASQGNQEVFDQSIRNLHLAIDKARDKVISKDHPETPLVGEVDALTDLLYLTYGSF
                     VLMGVDPKPLFDTVHEANMGKIFPDGKAHFDPVTHKILKPDDWEEHFAPEPSIRRELD
                     SQIQKSLNRK"
     gene            6030..6992
                     /locus_tag="VBD27_000355"
     CDS             6030..6992
                     /locus_tag="VBD27_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO7024392.1"
                     /translation="MKKILVLHTGGTISMNANEKGQVMSSADNPMKYVDLSLDDLDLT
                     VVDFLNLPSPQITPHHMLDIYHYLKQHASNFDGVVITHGTDTLEETAYFLDTMILPKI
                     PIIITGAMRSTNELGSDGVYNYLSALRVANSTKAADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGIIMKQDLLFFKATEERVRFDLDKITGTVPIVKAYAGMGDSGII
                     SFLNSQNISGLVIEALGAGNMPPKAAQEIEELIEQGVPVVLVSRCFNGIAEPVYGYEG
                     GGAKLQESGVMFVKELNAPKARLKLLIALNAGLTGQNLKDYIEG"
     gene            complement(7027..7905)
                     /gene="aroE"
                     /locus_tag="VBD27_000356"
     CDS             complement(7027..7905)
                     /gene="aroE"
                     /locus_tag="VBD27_000356"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO7024393.1"
                     /translation="MNSLNGETLLTGLIANPARHSLSPLMWNTSFQEKNMNYAYLTFE
                     VEEGKLTEAVRGVRALSIRGVNVSMPFKQSVIPLLDDLSPQAKLVGAVNTIVNQGGTG
                     RLVGHMTDGIGCFKALAAQGFSAKNKIITIAGIGGSGKAVAVQAAMEGVAEIRLFNRN
                     SSNYDKVIDLSDKIKKQFQIKVVVDYLENKTAFKDAIRTSHFYIDATSLGMRPLDNYS
                     LINDPEILTPNLVVVDLVYKPKETALLRFVRQNGVKHAYNGLGMLIYQGAEAFQLITN
                     QEMPISSVERVLQTEN"
     gene            complement(8042..8917)
                     /locus_tag="VBD27_000357"
     CDS             complement(8042..8917)
                     /locus_tag="VBD27_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7024394.1"
                     /translation="MLQNIGQTGIQATRIALGCMRMSDLKGKQAEEVVGTALDLGINF
                     FDHADIYGGGLSELRFRDAIKHLNVNRDKMIIQSKCGIREGYFDFSKEYILSSVDGIL
                     ERLGTEYLDFLILHRPDVLVEPEEVAEAFTKLRAEGKVKHFGVSNQNRFQMELLQSYL
                     DEPLAVNQLQLSPAHTPMFDAGLNVNMLNKASIEHDDGIVDYCRLKRVTIQAWSPFQI
                     DLSRGLFVNHPDYKELNETIAKLAKNYNVSSEAIVIAWILRHPAKMQAIVGSMNPSRL
                     KAIDKANDIALTRKE"
     gene            complement(9208..11223)
                     /gene="recG"
                     /locus_tag="VBD27_000358"
     CDS             complement(9208..11223)
                     /gene="recG"
                     /locus_tag="VBD27_000358"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO7024395.1"
                     /translation="MLLQSPISNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVSQGISQSTLIKAIKSAFE
                     INAHLELKENLPATLLGKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLSILYSKHAMETKISSLPFILTNAQKRSLDEILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIITRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARRVASDIVKDNNWKENTEWALILDNLRQHSDFD"
     gene            complement(11303..12841)
                     /locus_tag="VBD27_000359"
     CDS             complement(11303..12841)
                     /locus_tag="VBD27_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7024396.1"
                     /translation="MSKQKVTATLLLSTLVLSLSSPLVTLAETINPETSLTMATASTE
                     SSSEAEKQEKTQPTDSETASPSAEGSISTEKTEIGTTETSSSNESSSSSSHQSSSNED
                     AKTSDSASTASTPSTNTTNSSQADSKPGQSTKTELKPEPTLPLVEPKITPAPSQIESV
                     QTNQNASVPALSFDDNLLSTPISPVTATPFYVEHWSGQDAYSHYLLSHRYGIKAEQLD
                     GYLKSLGIQYDSNRINGAKLLQWEKDSGLDVRAIVAIAVLESSLGTQGVAKMPGANMF
                     GYGAFDHDSSHASAYNDEEAIMLLTKNTIIKNNNSSFEIQDLKAQKLSSGQLNTVTEG
                     GVYYTDNSGTGKRRAQIMEDLDRWIDQHGGTPEIPAALKALSTASLADLPSGFSLSTA
                     VNTASYIASTYPWGECTWYVFNRAKELGYTFDPFMGNGGDWQHKAGFETTHSPKVGYA
                     VSFSPGQAGADGTYGHVAIVEEVKKDGSVLISESNAMGRGIVSYRTFSSAQAAQLTYV
                     IGHK"
     gene            complement(12934..14034)
                     /locus_tag="VBD27_000360"
     CDS             complement(12934..14034)
                     /locus_tag="VBD27_000360"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO7024397.1"
                     /translation="MISSYHRPTRALIDLEAIANNVKSVQEHIPSDKKTFAVVKANAY
                     GHGAVEVSKYIESIVDGFCVSNLDEAIELRQAGIVKMILVLGVVMPEQVILAKNENIT
                     LTVASLEWLRLCQTSAVDLSGLEVHIKVDSGMGRIGVRQLDEGNKLISELGESGASVK
                     GIFTHFATADEADNCKFNQQLTFFKDFISGLDNCPDLVHASNSATSLWHSETIFNAVR
                     LGVVMYGLNPSGTDLDLPYPINPALSLESELVHVKQLHDGSQVGYGATYQVTGDEFVG
                     TVPIGYADGWTRDMQGFSVIVNGELCEIIGRVSMDQMTIRLPQKYTIGTKVTLIGQQG
                     SCNITTTDVAQKRQTINYEVLCLLSDRIPRYY"
     gene            complement(14031..14390)
                     /locus_tag="VBD27_000361"
     CDS             complement(14031..14390)
                     /locus_tag="VBD27_000361"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEO7024398.1"
                     /translation="MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNP
                     KRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSI
                     SHSGNYAQASVILEEEK"
     gene            complement(14415..15422)
                     /locus_tag="VBD27_000362"
     CDS             complement(14415..15422)
                     /locus_tag="VBD27_000362"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO7024399.1"
                     /translation="MGFHQLSDKINIEILKQKTSLDLEVSQKKLAKEEELKNIIKGED
                     QRFLVIVGPCSADNPKAVLTYAKRLAKLEAAFKDKMFLVMRVYTAKPRTNGDGYKGLV
                     HHSDKLGVFFQARKMHYDIIRETGLLTADELLYPEMLSVMDDLVSYYAIGARSVEDQG
                     HRFISSGIDAPVGMKNPTSGNLRVMFNAVYAAQNQQELFYQNKQVRTDGNLLSHVILR
                     GYHNADYRSIPNYHYENLLETITHYEETDLQNPFIVVDTNHDNSGKQFLEQIRIVKSV
                     LADRQWHTKIRNYVRGFLIESYLEDGRQDKPDVFGKSITDPCLGWDKTEMLIRDIYNS
                     L"
     gene            complement(15548..18076)
                     /gene="secA"
                     /locus_tag="VBD27_000363"
     CDS             complement(15548..18076)
                     /gene="secA"
                     /locus_tag="VBD27_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO7024400.1"
                     /translation="MANILRTVIENDKGELKKLDKIAKKVDSYADHMATLSDEALQAK
                     TPEFKERYQNGETLDQLLPEAFAVVREASKRVLGLYPYHVQIMGGIVLHHGDIPEMRT
                     GEGKTLTATMPVYLNAISGLGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PFEKREAYNCDITYSTNAEVGFDYLRDNMVVRQEDMVQRPLNYALVDEVDSVLIDEAR
                     TPLIVSGPVSSEMNQLYTRADMFVKTLNSDDYIIDVPTKTIGLSDTGIDKAENYFHLN
                     NLYDLENVALTHYIDNALRANYIMLLNIDYVVSEEQEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKESVPIQEESKTSASITYQNMFRMYHKLAGMTGTGKTEEEEFREIYNMRVIP
                     IPTNRPVQRIDHSDLLYPTLDSKFRAVVADVKERYEQGQPVLVGTVAVETSDLISRKL
                     VAAGVPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGTDRIKVVLERMNLAE
                     DDTVIKSKMLTRQVESAQRRVEGNNYDTRKQVLQYDDVMREQREIIYANRREVITAER
                     DLGSELKGMIKRTIKRAVDAHSRSDKNTAAEAIVNFARSALLDEEAITVSELRGLKEA
                     EIKELLYERALAVYEQQIAKLKDPEAIIEFQKVLILMVVDNQWTEHIDALDQLRNSVG
                     LRGYAQNNPIVEYQSEGFRMFQDMIGSIEFDVTRTLMKAQIHEQERERASQHATTTAE
                     QNISAQHVPMNNESPEYQGIKRNDKCPCGSGMKFKNCHGLRCLQ"
     gene            complement(18185..19132)
                     /gene="manA"
                     /locus_tag="VBD27_000364"
     CDS             complement(18185..19132)
                     /gene="manA"
                     /locus_tag="VBD27_000364"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEO7024401.1"
                     /translation="MSEPLFLEASMHDKIWGGTKLRDEFGYDIPSETTGEYWAISAHP
                     NGVSRVKNGRFKGCFLDKLYQGEKSLFGNPDDTVFPLLTKILDANDWLSVQVHPDDAY
                     ALKHEGELGKTECWYIISADEGSEIIYGHNAKTKEELRQMIESGDWEHLLTRIPVKSG
                     DFYYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRPDASGKLRDLRIEQSIDVLTI
                     GKPANTVPANMKLKHLSSTLLVSNDFFTVYKWEISGVTNFKQFAPYLLVSVLDGAGHI
                     TVDNKVYTLKKGDHFILPNDVVKWDIDGQLEIIASHVKK"
     gene            complement(19250..20131)
                     /locus_tag="VBD27_000365"
     CDS             complement(19250..20131)
                     /locus_tag="VBD27_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO7024402.1"
                     /translation="MTKLYGSIEAGGTKFVCAVGDEELKVVEKMQFPTTTPQETIKKT
                     VDFFKRFEKKLEAVAIGSFGPIDIDKKSKTYGYITTTPKLHWANVDLLGLISKDFNVP
                     FYFTTDVNSSAYGEVIARNNIDSLVYYTIGTGIGAGAIQKGEFIGGTGHTEAGHTYMA
                     MHPQDQANDFKGICPFHNSCLEGLASGPTLEARTGIRGELIEENSMVWDVQAYYIAQA
                     AIQATVLYRPQVIVFGGGVMAQEHMLRRVRQTFATLLNGYLPVPDLSDYIVTPAIEEN
                     GSATLGNFALAKKISKG"
     gene            complement(20199..22118)
                     /locus_tag="VBD27_000366"
     CDS             complement(20199..22118)
                     /locus_tag="VBD27_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO7024403.1"
                     /translation="MASNTEIAKQVINAIGGASNVRSVAHCATRLRIMVKDETVIDKN
                     TVENIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVAQGLPTLSTSDQKAEAAKQGNAF
                     QRAIRTFGDVFVPLLPAIVATGLFMGIRGAINNDTVLALFGTTSKAFSSSNFYTYTVV
                     LTDTAFAFFPALISWSAFRVFGGNPVIGLVLGLMMVNSALPNAWAVASGDAHPIKFFG
                     FIPVVGYQNSVLPAFFVGLLGAKLEKWLHKKIPDVLDLLLVPFLTFTVMSILALFVIG
                     PIFHSVENYVLAGTKFVLNLPLGLSGLILGGVHQIIVVTGVHHIFNLLEAQLIAADGK
                     DPFNAIITAAMTAQAGATLAVGVKTKNKKLKALAFPAALSAGLGITEPAIFGVNLRFG
                     KPFIMGLIAGAAGGWLASILKLAGTGFGITIIPGTLLYLNGQIVKYLIMVIGTTSLAF
                     VLTYMFGYEDKDEKAVAEVSPLVEETDDDPTITQTSQLRAETIVSPLDGQVIALDTVS
                     DPVFSSGIMGDGLAIKPRGNTIYSPVDGFVQIAFETGHAYGIKSDKGAEILIHIGIDT
                     VTMNGTGFTSKVKADQKVKKGDILGTFDSAKIAEAGLDNTAMIIVTNTADFADVIPLV
                     KEGVVTTGDMIIDVK"
     gene            22303..23742
                     /locus_tag="VBD27_000367"
     CDS             22303..23742
                     /locus_tag="VBD27_000367"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEO7024404.1"
                     /translation="MNLPTEIRYRPYDEWTEEDKENILKNVSKSPWRATYHLEAKTGL
                     LNDPNGFSYFNGKFHLFYQNWPFGAAHGLKQWVHTESDDLVHFKETGIKLKPDHVNDS
                     HGAYSGSALAIDDKLFLFYTGNVRDMKWNRDPRQIGAWMTSDGKITKFDKVLISQPND
                     VTEHFRDPQIFNYDNQFYAVIGAQNSKKCGFIKLYKALNNDIHHWEFVGDLDFGGTGS
                     EYMIECPNIIFVKGKPVLLYSPQGLDKNELDYQNIYPNTYKIGQYFDANSSKIVEPSP
                     IYNLDYGFEAYATQGFNTSDGRAFIVSWIGLPDIDYPSDQFDYQGAMSLVKELSIKNG
                     NLYQYPVPAMKNLRQHQAEFKTQLQTNNTYELELLVPRNDLSSFVLFANPKGQGLSIT
                     IDTAKGKVIVDRSQAGQQYATEFGTSRQCDIPKDATSINIFIDKSIFEIFINKGEKVF
                     TGRVFPDAEQSGIQLKEGHVHGKYFELKY"
     gene            23744..>24697
                     /locus_tag="VBD27_000368"
     CDS             23744..>24697
                     /locus_tag="VBD27_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7024405.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVTKVNEAMRTL
                     GYKPNNLARSLQGKSAKLIGLIFPNIRNIFYAELIEHLEIELFKHGYKTILCNSEKDP
                     IKEKEYLEMLGANQVDGIISSSHNLGIDDYEKVEAPIVAFDRNLAPHIPIISSDNFFG
                     GKMAAQTLKKHGCQKMIMITGNDNSDSPTGLRRLGFSYESKESKVITVTNGLSNMRRE
                     MELKSIISTHKPDGIFTSDDLTALLVIKLISQLGLSIPEDIKVIGYDGTSFIQDYVPH
                     LTTIKQPIREIAQLMVEILLAKIEGQKTNKDYILPVSLIPGS"
BASE COUNT         7908 a         4928 c         3980 g         7881 t
ORIGIN      
        1 ttttgttaaa ctttcttcga aaacgttttc gaatataagt atcggaggtt gtaatatgac
       61 aattaaaaat ctcactgttg caggaagtgg cgttctaggt agtcaaatcg cctttcaagc
      121 agcttacaaa ggaatgtctg tcaccatata tgacattaac gatgaagctt taaataaggg
      181 aaaagaacgc atcaaaaaat tagctaaagt ctatcaatca gaaattgaaa cagcaaaaga
      241 agcatatagt gataaagcta aaagtatcaa gtataataaa aacttattgc ctagcttaga
      301 ccatattttt ctttcaaaag ttgccgattc attagacttg attgctgatt taccaaacca
      361 aattactttt tcaaaaaact tagaccaagc tgtaagtgac gccgatttag taatcgaagc
      421 tgtcccagaa acagttagta taaaagaaga tttttacaaa caattagcta aagtggcacc
      481 ttcaaagaca atctttgcaa caaattcttc aacacttgtt ccaagtcagt ttgctgatat
      541 aacaggacgg cctgacaaat ttttagcaat gcatttcgct aacaatattt ggcaaaataa
      601 tattgttgaa attatgggac ataagggaac tgacgacgag gttgtcaaag aagctttggc
      661 attctcaaaa gatattggta tggtaccttt atatattcat aaagaacaac ctggctatat
      721 tcttaattca atactagttc cattcttaga gagtgcatta gcattgtact ataataaggt
      781 ttctgattct gaaactattg ataaaacctg gaagttagga acaggagcac cgatgggacc
      841 tttagaaatt ttagatatta ttggcatcga taccgcttat aatatcatga agaattattc
      901 agataccacc agcgatccta atagtttaca tgctcacctt gcaaaaatgc ttaaagaaga
      961 attcattgat aaaggacgta ccggtaaagc tgctggacac ggcttttatg attacgacta
     1021 aactataaaa gaggttagat aaaaatctaa cctcttttat aattcaacta aagaatagtt
     1081 gcaccaagaa cagttttaaa atgatttaaa gcccattgat gattactatc atttaagctg
     1141 gctacagcag ctgctgggac ctcaatttta taacctaagt tataagcatc aattgctgtg
     1201 tgtaatacac aaatatctgt cagtactcct gtcaatatca aagtatctac tcgtctttca
     1261 cgtaggcgaa tatctaaatc agtaccagaa aaggcagaat aatggcgctt atctatccaa
     1321 aaaacacgac tatcttcctt aatagtttca tatagagttc ccaaaggacc atataggttt
     1381 ctaccagaag tccctttaat attatgctca ggaaaaagat tggtctctgg atgaaaaaca
     1441 tctccaatat catgattatc aatcgcaaaa aaaatataat cccctgaccg gtatgctttc
     1501 tctgtaacgt cagctatagc tgaagcgata gattgcgctg gtttacctgc cgttaatttc
     1561 ccatcatctg ccacaaaatc atatgtgtag tctattgaga tgagtgcttt tgtcattttt
     1621 cttcttatct cctcttgatt tcaaaaaagg aactgaaagc tactcccagt cccatgctat
     1681 gattaattat attcttttaa tttgtcaaaa ataccttcgt taataacttt gaggtatgtt
     1741 cccttcatac cgagtgagcg gctctcaatg attcccgcag actccaattt acgaagagca
     1801 tttacaataa cggaacgtgt aattccaata cggtctgcaa taacagatgc cgttaggcgt
     1861 ccttcaagtc catctaattc tcctaagata gcagctacag ctttcatttc tgaataagat
     1921 agagtgttga tagccattgt caccgctgtt tgtttacgaa tattttcttc tagattttct
     1981 gtttgaaggt taagtaattg aataccaaca acggtgcttg caatttcaac taaaatcaaa
     2041 tcatcgtcgc taaattcttt atcattacgc cagataataa atgttcccaa gcgcattcca
     2101 ccaccataaa ttggagcgat tgttgtaata ccatcttgga agttttcctt tgtctcaact
     2161 gggaaaattg acaaatcatt atctactgat aagttagctt ctgtatcata aacacgacta
     2221 gcagatttaa catagtaatc tgggaattgt tttgtttcaa aaaattcttc cacacggtca
     2281 gtgtttgttt tatatttcat ggcatagcca agtaagttac caccaccatt aataatacat
     2341 gcgttacagt caataatatc agctaattga gccgccattg tattatatgg taattcggca
     2401 tctaagctat ctacggaacg ctgtaagatg gatgtaattt ttctagtttt ttctaataaa
     2461 ttcggcattt ccttcatcct ttgtatgttt tgcgttacaa ttatatcaaa gcattttagc
     2521 ttttgcaaat cgatatcagt acattttaag aacttctatt gttaatttaa tgtttatcta
     2581 ataaaaattc attctagcgc ttgtattggc ttaaaaagcg agccattttt tcttgtagtt
     2641 cagtcaattc atcaacacgt ggtaagtaaa caattctaaa atggtcagcg gtattcatat
     2701 taaatcctct accgtgtgtt agtaatactt tttcttgttt taagaaatta agcacaaact
     2761 cttcatcatt atcaatacgg tacatatctg tatcaatttt agggaaaaga tacagtccag
     2821 catttggttt tacagcagat aaaccaggta tttcattaat tgctttatgg atataattac
     2881 gttgctcgta gatacgacct cctggcaaca acatactatc aatagattgc tgtccgccta
     2941 acgatgtttg aataacctgt tgagcaagaa catttgaaca cagcctcatg ttagctaaca
     3001 tattcaaacc ttcaatataa cctttaacat gttgtcgcgg accagacaat accatccaac
     3061 caacgcggaa accacagatg cgatgtgatt tagataagcc agataaagta actgtaaaaa
     3121 tatcctctgc aatagaagca attgggatat gctccattcc atccataact aaacggtcat
     3181 aaacctcgtc tgaaaaaata atcaagtcat tttgtctagc gatatccaca atctcttgca
     3241 gaatttcacg cggatagact gctccagttg gattatttgg attaatgagg acgattgctt
     3301 ttgttttgga tgtgattttt gatttgatat catcaatatc aggataccaa ttagcttcct
     3361 catcacaaat ataatgcaca gcatttcctc cagcaaggct aacacaagct gtccataacg
     3421 ggtaatctgg cattggaact agaacctcgt catcattatc aagcaaagct tgcattgaca
     3481 tagagattcc ctcactaaca ccattaacaa tgtatatatc atccatgtca acatggatgt
     3541 tttgtaactg gtaatactgc ataacagcct tacgagctga aaaaatacct ttcgaatcag
     3601 agtaaccttc tgattcacgc gcattcgtaa ttaaatcacg aatgacttca tctggcgctt
     3661 cgaagccaaa ggcagctgga tttccagtat ttaaccgcaa tatcttttca ccatttgccc
     3721 tcatgcggtc cgcctcttct aaaacagggc ctctaatatc ataagccaca tgttccagct
     3781 tcattgattt atcaaatatc ttcataaaaa tacctcgtat cttccattat aaaacaaata
     3841 ttctcaaaat tgaaacattt actgcggttt atcaagcgat taagttaata ttaagctatt
     3901 tcagaaaact aacataaaac gctttcattt ttctagaaaa aacgttataa tatagctata
     3961 aaatgaataa ccaacatatc taacttactc atcactcact ccttctgata agatggctag
     4021 ttgagtgcaa gaggttatct cttttggtta tatttagagg aggacaggct tatgtcacaa
     4081 aaatatgaac gcatccttgt cgctattgat ggatcaacag aatctgaact tgcttttgaa
     4141 aaggcagtta acgttgctct tcgcaatgat tctgaattaa tcttgacaca tgttatagat
     4201 acacgtgctc tacaaagctt tgcgacattt gatacttata tctatgaaaa attagaaaaa
     4261 gaagcaaaag atgtccttga ggaatatgaa aaacaagctc gtgaaaaagg agcagataaa
     4321 gttagacaag tcattgaatt tggtaatccc aaaacattac ttgcacatga tattccagaa
     4381 aaagaaaaag tagacttgat tatggttggt gcaacaggtc ttaatacctt tgaacgtttc
     4441 cttatcggat cttcctcaga atacatcctt cgtcacgcta aagtagattt actcattgta
     4501 cgtgacccta ataaaacaat gtaaatttaa tacgctcact ttctaaaata tcctgagtta
     4561 tattaactca ggatattttt attttcgatt taaggatttc tgaatttggc tatctaattc
     4621 acgtcgaatt gatggctcag gagcgaaatg ttcttcccag tcgtctggtt ttaaaatttt
     4681 atgagtaaca ggatcaaaat gagctttgcc atctggaaag attttaccca tattggcctc
     4741 atgtactgta tcaaaaagag gttttgggtc gactcccata agaacaaagg agccataagt
     4801 cagataaagt aaatctgtta aggcatccac ttctcccact aatggtgttt ctggatggtc
     4861 tttagaaata accttatctc ttgctttatc aatagctaag tgtaaattac ggatagattg
     4921 gtcaaatact tcttggttac cttgactagc agcatacaag aactcaacga tttcttctac
     4981 tttaaagtcc gacctaaaac cagcttcctt actgtcatat aagcgtggcg ttttaatagt
     5041 ttcaccatcc attaatagat ggaaagattt tactttattg aaattctcgt cacgactact
     5101 gaatgttttt tcaatctcaa attggataag tccataatgg gctaatgcct tagagatacc
     5161 atcatcatta ttgtcagcag ttgtataacg tgcattttct ttcacttgct gactagcgtt
     5221 ccccatggca acaccaatac caacttgaga taacatctca atatcattat cagaatctcc
     5281 aaatgctatc acttctgata aatcaaaacc atagcattga ccaacacgtt caattccctt
     5341 aacttttgac tgcccttcag aaatgacatc cattgagtaa ggactgcttc ttgttaattt
     5401 aacacgagga aattgttttt gaattcgctc tgattcgccc tctgttgcaa ctaaaacgac
     5461 ctgataaata ggttctcgta agataaccat gaggctatca atattttgag gtttaatccg
     5521 acgaataaaa tgcttaaagc ttcgttcaat cgcttttgcc cattttctcg gaacaaggct
     5581 agatacaatc tgccccaaac ggctagttcc tagaccaata atacctgaac cgagtaatcc
     5641 tgaggctgtt cctaatgaaa tttctcttct tcttgctcca gcataacgaa tcagacgata
     5701 gacagttgtc ttagataatt gattggtata aataatttca cttctactat agatatattg
     5761 accattatag gttacagcaa aatctaaacc aaggttttct aaaaaaggtt gtacaaaact
     5821 aggacctcgc cccgttgcta aaccgactag tattccttgg tctttcaaat ttcgaattgc
     5881 tttaattgtt gacttttgta cattcttacg atcattaaga agtgtaccat caatatcaaa
     5941 aaatactgcc ttaattgcca ctgggtcaac atcccctctt ttttatgata aaataaatta
     6001 tatcacaact tgaaagaagt ataaaatcaa tgaaaaaaat ccttgttcta cacacaggtg
     6061 gaacgatttc catgaatgct aacgagaagg gacaggtcat gtccagcgcc gataacccaa
     6121 tgaaatacgt tgatttatca ttagatgacc ttgatttgac tgttgtagat tttctaaatc
     6181 ttcccagtcc tcaaatcacc cctcatcata tgcttgatat ctatcactat ctgaagcaac
     6241 atgccagtaa ttttgatgga gttgtcatta cgcatgggac tgacacttta gaagaaacag
     6301 catatttttt agacacaatg atactcccta aaatcccaat catcattacc ggtgctatga
     6361 gaagcacaaa tgaacttggt agcgatggcg tttataatta cctatcagcc ctccgtgtcg
     6421 ctaatagtac taaagctgcc gacaaggggg tgctagttgt catgaatgat gagatacatg
     6481 ctgctaaata tgtcacaaaa acacatacaa caaacgtttc gacattccaa acccctacac
     6541 atggccccct tgggattatt atgaaacaag acctgctttt ctttaaagca actgaagaga
     6601 gagttcgttt tgatttagat aaaattacag gaactgttcc tatcgtgaaa gcatatgctg
     6661 gcatgggtga tagtggtatc attagctttt taaattctca aaatattagc ggattagtta
     6721 ttgaagcact cggtgcaggg aatatgcccc caaaagctgc acaagaaatc gaagaattaa
     6781 ttgagcaagg tgtccctgtt gttctagtgt ctcgctgttt taatggtatt gctgagccag
     6841 tttacggata tgaaggtggc ggagcaaaat tacaagaatc tggtgtcatg tttgttaaag
     6901 aactaaacgc tcccaaagcc agactcaaat tactcattgc actcaatgca ggtctcacag
     6961 ggcaaaatct aaaagactat attgaaggtt gataaaaaac tctagaaata tttctagagt
     7021 tttttattaa ttttcagttt gtaatactct ctcaacactg ctaataggca tttcttgatt
     7081 cgttattaat tgaaatgctt ctgctccttg ataaatcagc atccctagac cattataagc
     7141 atgtttcact ccattttgtc taacaaatcg taacaatgct gtttctttag gcttgtaaac
     7201 caagtcgaca actactaaat tcggtgttaa aatttctgga tcgttaatta aactataatt
     7261 atctaatggc ctcattccta aactagtagc atcaatataa aaatgactag ttctaatagc
     7321 gtctttaaat gctgtcttat tttctagata atcaacgact acctttattt gaaactgttt
     7381 tttaatttta tctgataagt caatgacctt atcgtaattt gagctgttac gattaaataa
     7441 tctaatttcc gcaactccct ccatagctgc ttgaactgca actgctttac ctgaaccacc
     7501 aataccagct attgtaatta ttttattttt agcactgaaa ccttgagctg ctaaagcttt
     7561 aaaacaacca atgccatctg tcatatggcc tactaaacgt ccggttccac cttgattaac
     7621 gatagtattt acagcaccca ctaatttagc ttgaggagat aaatcatcta gcaaagggat
     7681 aacactctgt ttaaatggca ttgaaacatt aacaccacga atactcaatg ccctgacacc
     7741 tcgaacagct tctgttaatt taccctcttc tacttcaaat gtcagatagg cataattcat
     7801 gtttttttct tgaaaagagg tattccacat taacggggat agagagtggc gtgcaggatt
     7861 ggcaattaga ccagttagta aagtttcccc atttaatgaa ttcattgtat aaccttcttt
     7921 cttaacaatt tatttcagtt cattttacca aaaactgact caattaaaaa gagagtatgt
     7981 aatttatact ctctccctta gtttaaggca atatattacc tgcactacgg taaatatcat
     8041 actattcttt acgggttaaa gcgatatcat tagccttatc aatcgccttt aaacgagatg
     8101 gattcattga accaacaatc gcctgcattt tggcaggatg acgtaaaatc catgcaatca
     8161 caatagcttc gctgctaaca ttgtagtttt ttgccagttt agctatggtt tcatttaatt
     8221 ccttataatc cggatgatta acaaataaac cacgagataa atcaatttga aatggagacc
     8281 aagcttgaat ggtaactctt ttaagacgac aataatcaac aatgccatca tcatgttcaa
     8341 ttgaagcttt atttaacatg ttaacattta accctgcatc aaacattggt gtatgtgctg
     8401 gcgataactg aagttgattc actgctaagg gctcgtcaag gtaggattgt aacaactcca
     8461 tttgaaaccg gttttgattg ctaacaccaa agtgttttac tttaccttct gccctcagtt
     8521 tggtaaatgc ttctgctact tcttcaggct caactaatac atctggacga tgaagaatga
     8581 gaaaatctaa atattctgtc cctaatcttt ctaaaatacc atctactgat gataaaatat
     8641 actctttaga aaaatcaaag taaccttctc gtatcccaca tttagattga ataatcattt
     8701 tgtcacgatt gacatttaaa tgtttgattg catctcgaaa gcgtaattca gatagccctc
     8761 ccccataaat atcagcatga tcaaaaaaat taatacccaa atctaatgct gttccaacta
     8821 cttcttcagc ttgttttcct ttcaagtcac tcattctcat acaacctaaa gcaattcgag
     8881 ttgcttgaat gcctgtttgt ccaatgtttt gtaacatatg caccccattc taatctgttt
     8941 gtattaacag aaagattaat caattcatta taacaaaatt tctatgaaaa ggcttactat
     9001 tacaaatgca aaaagctgag taaatttaac tcaacttttt ttctgatact cattattgac
     9061 taaataagtc ggtaagaaaa atctattctg aaaagtacta ttataaatta cagtaatcat
     9121 tattgtcata acatacctcc acatctgtga taggaatatc atatgacagc aagcttaaac
     9181 tgagcttagc acagccttaa tagaaactta atcaaaatct gaatgttggc gtaaattatc
     9241 tagaataagt gcccattctg tattttcttt ccaattatta tctttgacaa tatcactagc
     9301 aacacgtctg gcttcttcta aaataggata atcctcaacg atatcagcta cttgaaattc
     9361 tggaattcca gactgacgag taccaaagat ttcaccagaa ccacgcattt ttaaatccga
     9421 ctcagctaaa acgaaaccat ctgtcgtttc tgtcatgatt gtcattcgtt ttttcccaga
     9481 atcagttttg ggattagcca ctaaaacagc gtatgattgt ttatatccac gaccaacacg
     9541 cccacgaagt tgatgtagct ggcttaatcc aaaacgatcg gcatccataa taatcatgat
     9601 cgttgcattt gggacattta cccctacttc aataactgtt gtagatacta aaatatgact
     9661 ttttttatcc ttgaaatctt gcattatagc atctttttca tcatttttca tacgtccatg
     9721 tacaagagca accttagcaa ttccttcaaa ataagtagat aattcagcat gcaatgctac
     9781 tgcattcttt aaatctaaag cttctgactc ttcaatcaac ggtgaaatga catacacttg
     9841 agcatctttt tgcaattcac cttttaccca ttccaacaca gtacctagct gctcgtgttt
     9901 cacccagcgt gtaataatag gtttacgacc ggcaggtaat tcatcaataa tagaaacatc
     9961 catttctcca aaagctgtga ttgctagagt tcggggaatt ggggtggctg tcatcattaa
    10021 aacatcagga ttttctccct tttcacggaa aattctacgc tgtttaacac caaaacgatg
    10081 ttgctcgtct gtaattacaa gccccagttt atggtactgt accgagtcct ggataagagc
    10141 atgagttcct acaatcatat caaccgagcc atttgcaata gctgctaaaa ccgtacgctt
    10201 aacagctgct ttcatacccg aagttaatat agcgattgat aaatctggaa ataactcttg
    10261 cagactaatg tagtgttgtt cagctaggat ttccgttgga accatcaaag ccgattgaaa
    10321 acctgctgta taagctgcat acattgatag accagcaatg actgtctttc ctgatcctac
    10381 atctccttgc aataaacgat tcatatgagc tcccgatgac atatcagata atatttcatc
    10441 taaagagcgc ttttgagcat tcgttagaat aaaaggtaaa gatgaaatct ttgtctccat
    10501 agcatgttta ctataaagaa tagacaaacc atttgtttca gatttatttt cggctttcaa
    10561 aacttgaagg ttcatttgaa agtaaaataa ttcttcaaat ttaatccgac ggagcgcttg
    10621 cttatactct gtgatatctt ttgggaaatg catagctaaa caagcctgac tacgacccat
    10681 caatcggtat tttcccaata aagtagctgg taaattttcc ttcaattcca aatgcgcatt
    10741 gatttcaaaa gctgacttaa tagctttaat caaagtagac tgtgaaattc cctgagatac
    10801 gcgataaaca ggttgcatgt catcttcaac ttgagctaaa accttcatcc cagtaatagc
    10861 tgatttagtt gcatcccatt taccaaagac agctacctct tgaccaagtt ctattttatc
    10921 agctaaatag ggttgattaa aaaaactaac atttaagaca gcttcccctt gacgcaattt
    10981 gaaacttaaa cggttacgtt taaaaccata atattgtaca ttagctggag taacgactaa
    11041 gcctgtaatg actgcttttt caccatccac taaatcaaaa acagattttg atttaaaatc
    11101 ttcatagcga aacggataat aaagaagtaa atcttctact gtataaatat ctaatttctg
    11161 aaatttttct gctgattttg gtccaaatcc cttaagatta gaaattggtg attgtaatag
    11221 cataagattc tccttctaat atcttttatt atactaaaag atcaccaaaa aggcgcctca
    11281 agagcgcctt tattatgtct aattatttat ggccaataac ataagttaat tgtgcagctt
    11341 gtgctgaact aaaagtacgg taagagacaa taccacgtcc cattgcatta gattctgaaa
    11401 tgagaactga accatctttt ttaacttctt caacaatagc tacgtgaccg taagtgccat
    11461 cagcaccagc ttgtcctggt gaaaatgata cagcatagcc tacttttggt gaatgtgttg
    11521 tttcaaagcc agccttatgt tgccaatctc caccattacc cataaatgga tcaaatgtat
    11581 aacctaactc tttagcgcgg ttaaagacat accatgtaca ttcaccccat ggataagttg
    11641 atgcaatata gctagctgtg ttaaccgctg ttgataagct aaaaccactt ggtaaatctg
    11701 ctaaacttgc tgtcgataaa gctttcaagg cagcaggaat ttctggtgtc cctccatgtt
    11761 gatcaatcca gcggtctaaa tcttccataa tctgggcacg acgtttacca gttccagagt
    11821 tatctgtata ataaacacca ccctcagtaa ctgtattaag ttgtccagaa gataatttct
    11881 gtgctttcaa atcttggatt tcaaagctag agttgttgtt tttaataatt gtattttttg
    11941 tcaacaacat aattgcttct tcatcattat aagcactagc atggctagag tcatgatcaa
    12001 aggcaccata accaaacata ttagcacctg gcatcttagc cactccttga gttcccaatg
    12061 aactttcaag gacagcaata gctacaatag cacggacatc taaaccacta tctttttccc
    12121 attgtaataa cttagcacca ttgatacgat tagaatcata ttgaatccct aaagatttta
    12181 agtacccatc taattgttca gctttgatac cgtaacgatg tgacaataaa tagtgagagt
    12241 aggcatcctg accagaccag tgttctacgt agaatggcgt tgctgtcact ggtgaaatcg
    12301 gtgttgataa taagttatca tcaaaggata aagcaggaac agaagcattc tgatttgtct
    12361 gaacactttc tatctgagac ggagcgggag ttattttagg ctctactaat ggtaaggtag
    12421 gctcaggttt taattcagtc tttgttgatt gacctggctt actgtctgct tgactactgt
    12481 ttgtagtatt agtgctagga gtagatgctg ttgaagcaga atcagatgtt ttagcatctt
    12541 cgttggaaga agattgatgt gatgaacttg atgatgattc attgcttgat gatgtctctg
    12601 tcgtaccaat ctctgttttt tctgttgaga tacttccttc ggctgaaggt gaagcagttt
    12661 ctgaatctgt aggttgtgtt ttttcctgtt tctctgcttc agaagaactt tctgttgatg
    12721 ctgttgccat tgtcaggctt gtttctggat taatagtttc tgctaaggtc actaaaggtg
    12781 atgatagcga taagactaaa gtggataaca acaaagttgc cgttactttt tgttttgaca
    12841 tatactaatg attcccttct ataaaaattg tcactgatta gtctaacata tttttaaatt
    12901 ttttaaaaca gttaactact gtgtcatttg tttttaataa tatctcggaa tacgatcgct
    12961 taaaagacaa agtacttcat aattaatcgt ctgtcttttt tgagcgacgt ccgtagttgt
    13021 aatgttacaa ctaccttgtt gaccaatcaa ggttacttta gttccgatag tatatttttg
    13081 aggtaaacgg atagtcattt gatccatcga gacacgccca ataatttcac aaagttctcc
    13141 atttactata actgaaaatc cttgcatatc tcgtgtccat ccatcagcgt agccaatcgg
    13201 tactgttcca acaaattcgt ctccagtaac ttgataggtt gcgccatagc caacttgaga
    13261 accatcgtgt agttgtttaa catgtactag ctcagactct aaacttaaag cagggtttat
    13321 agggtaaggt aagtctaaat cagttccact tggatttaag ccgtacataa caacacccaa
    13381 tctaacggca ttaaaaattg tctcactatg ccaaaggctt gtagcagaat tactagcatg
    13441 aacaaggtcc ggacaattat ctaatcctga aataaagtct ttaaaaaaag taagttgttg
    13501 attgaattta cagttatcgg cttcatcagc tgttgcaaaa tgggtgaaaa tccccttgac
    13561 agaggcacca ctttccccca attcagaaat tagcttattt ccttcatcta actggcgaac
    13621 acctatacgt cccattccac tatcaacttt gatatgtact tctagaccag ataaatctac
    13681 tgctgatgtc tgacataaac gtaaccactc caagctagca actgttagag taatattttc
    13741 atttttggct agtataacct gctcaggcat aacaacacct aataccaata tcatttttac
    13801 aattcctgct tgacgtagct caatagcctc atctaaattg gatacgcaaa aaccatctac
    13861 aatagactca atgtattttg aaacttcaac agcaccatgc ccataagcat tagccttaac
    13921 tacagcaaat gtttttttat cacttggaat gtgctcttgt acagatttaa cgttatttgc
    13981 aatcgcctct aaatctatca aggctctggt tggtctatga taacttgaaa tcatttttct
    14041 tcctccaaaa taacactagc ttgtgcataa ttaccactat gagatattga aacaaaagat
    14101 tttccattaa acggttcttt tgtaatcaaa ggcgctcctt tatcatccga taaaatttcg
    14161 atatcatgaa aattaacttt cccaattcct gttccaagtg ctttgctata agcctctttt
    14221 gctgcccatc gccctgttaa aaaagacatc tgacgcttgg gattggaaat tcctttaaaa
    14281 agaagcaatt cttgttcggt caaaacgcgt tctgcaaaac gttgattacg ctcatatgct
    14341 ttagtaatcg cctctatctc ttgtaaatca attccgtgtc caacaatcat gtgtaactcc
    14401 tataagttcg atacttacaa actattgtaa atatccctaa ttagcatttc tgttttatcc
    14461 caacctagac atggatctgt gatagactta ccaaaaacat ctggtttatc ttgtcttcca
    14521 tcttctaaat aagattctat caaaaaacca cgaacatagt ttctaatttt agtatgccac
    14581 tgtcggtctg caagtactga tttcacaatg cgaatttgtt ctaaaaattg cttacctgaa
    14641 ttgtcatgat ttgtatcaac aacaataaag ggattttgca aatcagtctc ttcataatga
    14701 gtaatagttt ctaaaaggtt ttcataatga taattaggga tacttcgata gtcggcatta
    14761 tgatatcctc tcaaaataac atgtgacagc aaattaccat ctgttctaac ttgcttattt
    14821 tgatagaata attcctgttg attttgtgct gcatagaccg cattaaacat gactcttaaa
    14881 ttaccagacg ttggattttt catacctact ggtgcatcta tacctgaaga tataaaacgg
    14941 tgtccctgat cttccactga acgcgcacca atagcatagt aagaaactaa atcatccatc
    15001 actgataaca tctctggata aagtaattca tcagctgtca ggagtccagt ctctcgaatg
    15061 atgtcatagt gcatcttacg tgcttgaaaa aaaactccta gtttgtcaga atggtgaact
    15121 aagcctttat aaccatcacc attcgttctt ggcttagcgg tataaactct cataaccaaa
    15181 aacattttat ctttaaaggc agcttccaat ttagctaaac gctttgcata agttagcacc
    15241 gcctttggat tatccgcaga acaaggacct actattacta gaaaccgctg atcctcacct
    15301 tttatgatat tttttaactc ttcttctttg gctaattttt tttggctaac ttctagatct
    15361 aagctagttt tctgcttcaa tatttcaata ttaattttat cacttaactg atgaaatccc
    15421 atgttttttc ctaatacaac ctaagtctat taataagctc tgctatacaa tatagtcgtc
    15481 atactattag acaagtaatg ataaaacagt cttctcagac tgttttattt ttattttaaa
    15541 atcattgtta ttgtaaacat ctcagaccat gacaattttt aaacttcatg ccagagccac
    15601 aaggacattt atcattacgt ttaatccctt ggtattctgg tgattcatta ttcataggta
    15661 catgttgtgc cgagatattt tgctctgctg ttgtcgtagc atgttgagaa gcacgttcac
    15721 gctcttgttc atgaatttga gctttcatca atgtacgtgt cacatcaaac tcaatcgaac
    15781 caatcatatc ttggaacata cggaaacctt ctgattgata ctcaacgatt gggttatttt
    15841 gagcataacc acgaagtcca acggagttac gtaattgatc taaggcatca atatgctctg
    15901 tccattgatt atccactacc atcaagatta agactttttg gaattcgata atagcttctg
    15961 gatcttttaa tttagcaatt tgttgctcat aaacagctaa ggcacgttca taaagaagtt
    16021 ctttaatttc agcctctttt agaccacgaa gttctgaaac tgtgatagct tcttcatcta
    16081 ataacgccga tcttgcaaaa ttaacaatag cttctgctgc agtattttta tcgcttctgc
    16141 tatgtgcatc aactgctctt ttaatcgtac gtttaatcat ccctttgagc tctgatccta
    16201 aatcacgctc agcagtaatc acttctctac ggttagcata aataatctca cgttgttcac
    16261 gcataacatc atcgtattgc aagacttgtt tacgtgtgtc atagttatta ccttcaacgc
    16321 ggcgttgagc tgattcaact tgtcgtgtca acatttttga tttaataact gtatcatctt
    16381 cagctaaatt cattcgctca agaaccactt taatacgatc tgtaccaaag cgacgcatca
    16441 aatcatcttc taacgaaaga tagaattgtg attcacctgg atctccttga cgacccgaac
    16501 gaccacgaag ctggttatca atacgacggc tttcatgacg ctctgtacca ataacacata
    16561 aaccaccaag ttcacgaacc ccttcaccaa gcttgatatc agtaccacga ccagccatgt
    16621 tggttgcgat tgtaacagca ccacgttgcc cagcattcat aatgatttga gcttctttaa
    16681 agtgattttt agcattcaaa acttcatgtg gtactccagc agcaactaat tttctagaaa
    16741 ttaagtctga cgtttcaaca gctactgtac caaccagtac aggttgtcct tgttcataac
    16801 gttctttgac atctgctacc accgctctaa atttagagtc aagagttgga taaagtagat
    16861 ctgaatgatc tatacgttga acaggacgat ttgttgggat aggaataacg cgcatattat
    16921 aaatttcacg aaactcttct tcctcagtct taccagtacc agtcattcct gctaatttat
    16981 ggtacatacg gaacatattt tggtaagtaa ttgaagcgct cgtttttgat tcttcttgga
    17041 ttggcactga ctcttttgct tcaatagctt ggtgtaagcc atctgagaaa cgacgtcctt
    17101 ccatcgtacg accggtaaat tggtccacaa ttaaaatctc ttgttcttca ctaaccacat
    17161 aatcaatatt taacaacata atatagttag ctcgaagagc gttatcaatg tagtgagtta
    17221 aggcaacatt ttcgagatca tacaagttat ttagatggaa atagttttca gccttgtcaa
    17281 tacctgtatc tgataaacca attgtttttg ttggaacatc aatgatataa tcatcgctat
    17341 taagcgtttt tacaaacatg tccgcacgtg tatacagttg gttcatctca gagcttacag
    17401 gacctgaaac aatcaatggc gtcctagctt catcgattaa tacagagtca acctcatcaa
    17461 ccaaagcata gttgagtggg cgttgcacca tatcttcttg gcgaacaacc atattgtctc
    17521 gaaggtagtc aaatccgact tctgcgttag ttgagtatgt gatatcacaa ttgtaagctt
    17581 cgcgtttttc aaatggtgac ttagctgcta agttaatccc tacagataat ccaagccaac
    17641 tataaacctc acccatttcg gtcgcatcac gtgttgataa gtactcatta acagtaataa
    17701 catgaacacc taatcctgag atagcattta gataaacagg cattgtcgct gttaaagttt
    17761 ttccctcacc agtacgcatt tctggaatat caccatggtg aagaacgata ccacccataa
    17821 tttggacgtg gtatgggtat aaacctaata cacgtttaga agcttcacga actaccgcaa
    17881 acgcttccgg taacaattga tctaaagttt caccattttg ataacgttct ttaaattctg
    17941 gagtttttgc ttgtaaagct tcatctgata acgtagccat atgatctgcg taagagtcaa
    18001 ctttcttagc tattttatct aattttttta attcaccctt gtcattttca ataacagtgc
    18061 gtagaatatt tgccattcta attcctttcg atactcttat caccacattt taccataaat
    18121 aatgttgtct ttcaataaat ggtcgtttta atcgagatta aaacaagtgg tatccccact
    18181 ctcattattt tttaacatga cttgctataa tttctaactg tccatcaata tcccatttga
    18241 caacatcatt tggcaagata aagtggtcac cttttttaag tgtataaact ttgttatcta
    18301 ctgtaatgtg acccgctccg tctaagacac taaccaaaag gtagggtgca aattgtttaa
    18361 agttggtaac accagaaatt tcccatttat aaactgtaaa gaaatcattt gaaactaata
    18421 aagttgaaga tagatgtttt aacttcatat tggcaggaac agtattggca ggtttaccaa
    18481 tcgtgagaac atctattgat tgttcaatgc gtaaatcacg taattttcct gaagcatctg
    18541 gacgatcaaa atcataaaca cgataagtag tatcactaga ttgctgtgtt tctaaaatta
    18601 atatcccttt acctatagca tgcattgtcc cacttggaac gtagtaaaaa tcccctgatt
    18661 taacaggaat tcgggttaaa agatgttccc aatctccaga ttcaatcatt tggcgaagtt
    18721 cttctttagt tttagcatta tgaccataga taatttcaga tccttcatca gcagaaataa
    18781 tataccaaca ttctgtttta ccaagttcac cttcatgttt aagagcataa gcatcatccg
    18841 gatgaacctg tacactaagc cagtcattag catctaagat tttagttagt aaaggaaaga
    18901 cagtatcatc agggttgcca aataagcttt tttcaccttg atacagctta tctaaaaaac
    18961 aacctttaaa gcgaccattc ttaactcttg agacaccgtt aggatgagct gaaatagccc
    19021 aatattctcc tgttgtttca cttggaatat catagccaaa ttcatccctt aactttgttc
    19081 caccccagat tttatcgtgc atgcttgctt ctaaaaataa tggttccgac ataagaatcc
    19141 cctatctttt gtaaaattca agcctattat agcattttat ccatcatatc gcttactttt
    19201 tttaatcttt tatttgctaa ctaaactaag tagtggctta agggttttat taaccttttg
    19261 atattttctt tgctaaagca aaatttccta acgttgcaga accattttct tctatagctg
    19321 gtgtcacaat ataatcagac aaatcgggaa ctggtaaata cccatttaat aaagttgcaa
    19381 atgtctggcg aacacgtctt aacatgtgct cttgtgccat gacgcctcca ccaaatacaa
    19441 tgacttgtgg acgatataat acagttgctt gtattgcagc ctgagcaata taataggctt
    19501 gaacatccca aaccattgaa ttttcttcaa tcaattcacc acgaatacca gtcctagctt
    19561 ctagagttgg accagatgcc aaaccttcta aacagctatt atgaaacgga cagatacctt
    19621 taaaatcatt tgcttggtcc tgcggatgca tcgccatata agtatgacct gcttcagtat
    19681 gaccagttcc tcctataaat tcaccctttt gtatagctcc tgcaccaatt cccgtaccaa
    19741 tggtgtaata aacaagtgaa tcaatattgt tacgagctat aacttcacca taggcagagc
    19801 tattaacatc tgttgtgaaa taaaatggaa cattgaaatc tttagaaatt aagccgagta
    19861 aatcaacatt agcccaatgt aactttgggg tcgttgtgat ataaccgtat gtctttgatt
    19921 ttttatcaat atcaatggga ccaaaagaac caatagcaac tgcctctaac ttcttttcaa
    19981 atcttttaaa gaaatcaaca gtctttttaa tcgtttcttg aggcgtcgtt gttggaaatt
    20041 gcattttttc tacaactttt aattcctcat caccaactgc acaaacaaac tttgtcccac
    20101 ctgcttcaat actaccatat aactttgtca tcttttttcc tatctataat ctctatctca
    20161 aagacaaagg ttgggataat tatcccaacc tttaatcctc acttaacatc aataatcatg
    20221 tcacctgtag taacaacacc ctcttttacc aatggtataa catcagcaaa atcagctgta
    20281 ttagtcacaa taatcatagc tgtattatcc agacctgctt ctgcaatctt ggctgaatca
    20341 aaggtgccaa gaatatcacc ttttttcact ttttggtctg ccttgacttt agaagtaaac
    20401 cctgtgccat tcatggtgac agtatcaata cctatatgaa tcaaaatttc tgcaccttta
    20461 tctgatttga taccgtaagc atgacctgtt tcaaaagcaa tttggacaaa accatcaaca
    20521 ggtgagtaaa ttgtattacc tcttggttta atagcaaggc catctcccat aatacctgat
    20581 gaaaaaactg ggtcacttac tgtatcaaga gcaataacct gtccatcaag agggctaaca
    20641 atcgtttctg cccttaattg gctagtttga gtaattgttg gatcgtcatc tgtttcttca
    20701 accaaaggac taacttcagc tactgctttt tcatctttat cttcatatcc aaacatataa
    20761 gttaatacaa aagccaaaga cgtagttcca attaccataa taaggtactt aacaatttga
    20821 ccatttaaat aaagtaatgt tcctgggata atggtaatac caaaaccagt tcctgctaat
    20881 ttcaatattg aagctaacca gccacctgct gctcctgcaa ttaatcccat aataaatggc
    20941 ttaccaaaac gtaagtttac tccaaatata gccggttctg taataccaag tccagctgaa
    21001 agcgctgccg gaaaagcaag ggcttttaat tttttatttt ttgttttaac accaactgcc
    21061 aacgtggcac ctgcttgtgc tgtcatagca gctgtaataa tagcattaaa tggatctttc
    21121 ccatcagcag caattaactg agcttcaagt aaattaaaga tatgatgaac acctgtaaca
    21181 acgattatct gatgaacacc acctaaaata agacctgata ggccaagagg taaattaagg
    21241 acaaattttg tccctgctaa aacatagttt tcaactgaat ggaaaatagg accaatgaca
    21301 aaaagtgcta aaattgacat aactgtaaaa gttaggaacg gcactaatag taaatcaaga
    21361 acatctggga ttttcttatg caaccatttt tcaagtttag caccaagtaa acctacaaag
    21421 aatgccggca atactgaatt ttgataacca actactggaa taaagccgaa aaattttatg
    21481 ggatgggcat cacctgaggc cactgcccaa gcgtttggaa gggcagagtt gaccatcatc
    21541 aatcctaata cgagaccgat aacaggattt ccaccaaaaa ctctaaaagc tgaccaagaa
    21601 atcaaggcag gaaaaaaagc aaaggccgtg tcagtcaaaa cgacagtata ggtataaaaa
    21661 ttagaacttg aaaatgcttt tgaagtcgtc ccaaataaag ctaaaactgt atcattatta
    21721 atcgcaccgc gaatccccat aaataaacca gtagcaacaa tggctggcaa caatggaaca
    21781 aaaacatcac cgaaagtacg aatagctcgt tggaaagcat tcccttgttt agccgcttct
    21841 gccttttgat cagaggttga caaagttggc aaaccttgag ccactacttc atcataaatc
    21901 ttattaacag taccagttcc aaaaataatt tgatattgac ctgaattgaa aaatgctccc
    21961 tgaacttttt cgatattttc aacagtattt ttatcaatga ctgtctcatc ttttaccata
    22021 atgcgaagac gagtggcaca gtgtgctacg ctacgcacat tactagcacc accaatcgca
    22081 ttaatgactt gtttagcaat ttctgtattt gaagccatct ataaatctcc tttgacattt
    22141 tgaaagcgtt tcattttatt tactctaata ttctagcatg tctcataaat atatgtcaag
    22201 cgtttgacat aaaataatat tttttgatgt taagagttga tttcaaaaga gaaaacgttt
    22261 actatatagt tattagatat taagacagtc aaggagtatc aaatgaattt acccactgaa
    22321 atccgctatc gtccttatga tgaatggact gaagaagata aggaaaatat tttaaagaat
    22381 gtctcaaaat ccccttggag agcaacttat cacttagaag ctaaaacagg gcttttaaat
    22441 gacccaaatg gcttctctta ctttaatgga aaattccatc ttttttatca aaattggccc
    22501 tttggagctg cacatggttt aaaacaatgg gtacatacag aatcagatga tttagttcat
    22561 ttcaaagaaa caggaatcaa gcttaaacct gaccatgtca atgatagcca tggtgcatac
    22621 tccggttctg cattagccat tgacgataag ctttttcttt tttatactgg taatgtccgt
    22681 gacatgaaat ggaaccgtga cccccgccaa attggagcat ggatgacaag tgatggcaag
    22741 attacaaaat ttgataaagt ccttatttca caaccaaatg atgtcactga gcattttcgt
    22801 gatcctcaga ttttcaatta tgataatcaa ttctatgctg ttatcggggc gcaaaatagt
    22861 aaaaaatgcg gattcattaa attatataaa gcacttaata atgacatcca tcattgggag
    22921 tttgtgggag atttagattt tggtggtact ggatctgagt atatgattga atgtccaaat
    22981 attatttttg tcaaaggaaa acctgtatta ctttatagtc ctcaaggact agataaaaac
    23041 gaattggact accaaaacat ttatccaaat acctacaaaa tcggacaata tttcgatgct
    23101 aactcatcta agatagttga accatcccct atctataatt tagattatgg ttttgaagcc
    23161 tatgctactc aaggatttaa tacttctgat ggtcgtgcct ttatcgtgag ttggataggc
    23221 ttgccagata ttgattatcc tagcgatcaa tttgattatc aaggagctat gagtttagtt
    23281 aaagagttaa gcataaaaaa tgggaacctt tatcaatatc ccgttcctgc tatgaaaaat
    23341 cttcgtcaac atcaagcaga atttaaaaca caacttcaaa ctaacaacac ctatgaatta
    23401 gaattactag ttcctagaaa cgatctttct tcatttgttc tctttgctaa tccgaaagga
    23461 caaggactat ccattactat tgatactgca aaggggaaag ttatcgttga ccgctcacaa
    23521 gccggacagc agtacgccac agaatttggt actagtcgcc agtgtgacat tccaaaagat
    23581 gctactagta tcaatatttt tatcgataag tccatctttg aaatctttat aaataaagga
    23641 gaaaaagtat ttacgggacg agtctttcca gatgcagaac aatcaggaat tcaactaaaa
    23701 gaaggacacg ttcatggtaa atattttgaa ttaaaatatt aatatggttg caaaattaac
    23761 agatgttgct gcacttgccg gtgttagccc aacgactgta tcacgcgtta ttaacaaaaa
    23821 aggctattta tctcaaaaaa cagtcaccaa agtcaatgaa gccatgagaa cccttggtta
    23881 taaacctaat aatttagcac gtagcttaca gggaaagtct gctaaattaa ttggactcat
    23941 tttccccaac atccgtaaca ttttttacgc tgaattaatt gaacaccttg aaattgagtt
    24001 gtttaagcat ggttataaaa ctatcttatg taatagtgaa aaggatccta tcaaagaaaa
    24061 agaatacctc gaaatgctag gagcaaatca agtagacggt atcatttcaa gtagtcacaa
    24121 cctaggaatt gatgattacg agaaagttga ggctcccata gtagcattcg atagaaattt
    24181 agctccacac atcccgatca tctcatctga taatttcttt ggaggaaaaa tggctgctca
    24241 aacccttaaa aaacatggct gtcaaaagat gattatgatt acagggaatg ataattcaga
    24301 ttctcctact ggacttaggc ggctaggttt ttcctatgaa tcaaaggaat ccaaagttat
    24361 cacagtcaca aatgggctat ctaacatgag acgtgagatg gaattaaaat caattatctc
    24421 gactcacaaa ccagatggta tttttacatc agacgactta actgctttat tagtaatcaa
    24481 acttatcagc caactagggt tgtccatccc agaagatatc aaagtgattg gatatgatgg
    24541 tacaagtttc attcaagatt acgtcccaca tctaaccact attaaacaac ctattcgaga
    24601 aattgcgcaa ttgatggttg aaatactcct agctaagatt gaggggcaaa agacaaataa
    24661 ggattatatt ttaccagtca gtctcatccc tggtagt
//
LOCUS       DATRQA010000012        23549 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.012, whole genome shotgun sequence.
ACCESSION   DATRQA010000012 DATRQA010000000
VERSION     DATRQA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23549
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.012"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            713..2131
                     /locus_tag="VBD27_000369"
     CDS             713..2131
                     /locus_tag="VBD27_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HEO7024406.1"
                     /translation="MMTKETLAHYQKKIEQESEKKQSLDEHSWHVACFSRQEASIIGQ
                     GDVLFLIGLYHDLGKADRAFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKSRGVDKG
                     ERMTFNEMVGYVISAHHGMYDFCYCSDDAEYYSFNKFKNRINRDLDDYHYHEDIKGYA
                     IKLEEKLCDYGYKDLRELIDKAFDNYQQAMSSLNWQDNSEWDYYQSCMVRLYLSLLKN
                     ADILDTVNAYGLKISPMDKTERSSLKHSYLAAIEQKYASFGRPNNQLNTIRTEIAERV
                     KERGKRDSKGIYRLDLPTGAGKTNLSMRYAFHQLVHQDKSRFFYITPFLSVLEQNASE
                     IRKVTGDLGVLEHHSNMVKQANEDDDKDSLLSAYLIDSWDSQVVLTSMVQFFQTLFKT
                     KSANLRRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFFNKVMDTTIVLCTATQPAYD
                     SSEIDHRICYGGNLGELAEIVD"
     gene            2402..3361
                     /locus_tag="VBD27_000370"
     CDS             2402..3361
                     /locus_tag="VBD27_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7024407.1"
                     /translation="MLKVRYGVVSTAKVAPRFIEGVRLAGNGEVVAVSSRTLESAQAF
                     ANKYHLPKAYDKLEDMLADESIDVIYVATINQDHYKVAKAALLAGKHVLVEKPFTLTY
                     DQANELFTLAESCNLFLMEAQKSVFIPMTQVIKKLLASGEIGEVISISSTTAYPNIDH
                     VTWFRELELGGGTVHFMAPYALSYLQYLFDATITHASGTATFPKGQSDSQSKLLLQLS
                     NGVLVDIFLTTRLNLPHEMIIYGTEGRLIIPHFWKTTHAKLVRNDTSARTIQVDMVSD
                     FEKEAYHVSQMILEGQRVSHIMTPQLTLSGVKIIEGLYRSWGK"
     gene            3522..4424
                     /locus_tag="VBD27_000371"
     CDS             3522..4424
                     /locus_tag="VBD27_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7024408.1"
                     /translation="MFSSDFGFEIINVDDLSLQEEKFLPSELLAYARDENESSFVRDI
                     EGHLTLVYQLLDTQGHVDDVRHVPRVIPVTLFLKEDGLFVLANHKNIILVKKALNRVE
                     KVDSPKHLLLSLVTAFSKQYFDVLDTISEERDKLINDLRKRPNKSNLARLANLQSGTV
                     HLMMGTKQNFEMLTDLQNIEQDKENTRNEKMQLQDAIIEARQLSNMCSLNSQVFQELS
                     SSYNNVLSNNLNDNVTTLTIISIGISIIAMVTSFYGMNVKLPFDSVDAVWVLIILITT
                     IITIMLSIVMYIYVHKEDNHPKHK"
     gene            4584..5648
                     /locus_tag="VBD27_000372"
     CDS             4584..5648
                     /locus_tag="VBD27_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEO7024409.1"
                     /translation="MSIESDFFRKKRFIFSSLEEFGFIKSDQEYIYCQTFMDNDFKAI
                     ITISLDGKIAGKVIDSALEEEYLPLRAANYNGSFVGEVRSAYMAILGDISDSCCKDLL
                     FTKDQSNRLAEKIATTFEDSVDYPFAKHPQYASYRVSGKWYALLFPLKMGKLENVPAQ
                     LSEEEVEVLNIKVNPQDMEILLQKEGIYPSYHMSKKTWVSIVLDNTLSDIEIFKLVSD
                     SRKLVSPNKKSNSEPEFWIIPANPKFYDIDKEFAEHQLIDWTQKGKIAVGDYVFIYIT
                     APIRALRYVCQVIERDLKTSTYLGARDVKAYMRLKLIRQFEDSTYPVAFLAEHGVKTV
                     RGPRRMTKELRQVMAKTILE"
     gene            5763..6755
                     /locus_tag="VBD27_000373"
     CDS             5763..6755
                     /locus_tag="VBD27_000373"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEO7024410.1"
                     /translation="MKKSFIHQQQEISFVKNTFTQYLIDKLEIVEVQGPILSQVGDGM
                     QDNLSGIEHPVSVKVLNIPEAEFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPD
                     EDSLDPTHSVYVDQWDWEKVIPDGRRNLDYLKETVEKIYKAIRLTELAVEARFDIESI
                     LPKRITFIHTEELVEKYPDLSPKERENAIAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTTPSENGFKGLNGDILVWNEQLGTAFELSSMGIRVDEDALKRQVVLTGDEDRLEF
                     EWHKTLLRGFFPLTIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKEVKDTFENIL"
     gene            complement(6800..7249)
                     /locus_tag="VBD27_000374"
     CDS             complement(6800..7249)
                     /locus_tag="VBD27_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transport accessory protein"
                     /protein_id="HEO7024411.1"
                     /translation="MIKKLLLTLIAILCLTTLKSNLSSADTMTISQQQYQHMITNSDL
                     IAITDKALVSKSQQSEATIFIGRPTCQYCRAFLPKLLKSQATLHSKIYYLDSQKYKGK
                     RLKSFFKKHHITTVPNLAHYQRGKMTKYLVQGSQATPQQIQTFLSQK"
     gene            7215..7919
                     /gene="rsmD"
                     /locus_tag="VBD27_000375"
     CDS             7215..7919
                     /gene="rsmD"
                     /locus_tag="VBD27_000375"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO7024412.1"
                     /translation="MAIKVNNNFLIIMGTSFLLLLAYHIKKEGKEILSSLFVISNFFD
                     NSTLFFVIIVVMRVVAGTFGGRPLKTLDGKTTRPTTDKVKGAIFNMIGPFFEGGRVLD
                     LFSGSGSLAIEAISRGMDQAVLVEKDRRAQVVIQENIAMTKSPEQFQLLKIEANRALE
                     QLTGQFDLVLLDPPYAKEEIVKQIQIMDSKGLLGDDIMIVCETDKSVDLPEEIASFGI
                     WKQKIYGISKVTVYVR"
     gene            7931..8221
                     /locus_tag="VBD27_000376"
     CDS             7931..8221
                     /locus_tag="VBD27_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024413.1"
                     /translation="MDYKLVMIILILVILIYLLLRLHYLEELLKHYHFKNDEGVCSLS
                     RTKNPLYLLMAMITFLPNLINWIANGFEAIDIVRLIPVVVFLWLYYCGDDDE"
     gene            8218..8703
                     /gene="coaD"
                     /locus_tag="VBD27_000377"
     CDS             8218..8703
                     /gene="coaD"
                     /locus_tag="VBD27_000377"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO7024414.1"
                     /translation="MTKKALFTGSFDPVTNGHLDIIERASYLFDHVYIGLFHNLEKQG
                     YFSIECRKKMLEEAIRQFKNVSVLVAQDRLAVDLAREVGAKYFVRGLRNSQDFDYEAN
                     LEFFNKQLADGIETVYLSTSPSLSPISSSRIRELIHFKASVKPFVPKSVFREVEKMSE
                     E"
     gene            8693..9766
                     /locus_tag="VBD27_000378"
     CDS             8693..9766
                     /locus_tag="VBD27_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEO7024415.1"
                     /translation="MKNKDPKRKHKSLLGLLKWWIIGFAFLLLVLASLVVRLPYYLEM
                     PGGAYDIRSVLKVNKKADKAKGSYNFVAVSVSQATPAQVLYAWLTPFTELSSKEETTG
                     GFSNDDYLRINQFYMETSQNESIYQALKLANKQVSLTYKGVYVLNLAKNSTFKDRLHL
                     ADTVTGVNGKSFKNSSQLIKYVAALHLGDKVKVQYTSQGKKKESVGKVIKLSNGKNGI
                     GIGLTDHTEVSSDVPVDFNTEGVGGPSAGLMFTLAIYDQLVKEDLRKGRKIAGTGTIE
                     QNGHVGDIGGAGLKVVSAAKKGMDIFFVPNNPIDKNAKKGKTKVQTNYQEAKAAAKRL
                     GTKMKIVPVQNVQQAIDYLKKTK"
     gene            9841..11175
                     /locus_tag="VBD27_000379"
     CDS             9841..11175
                     /locus_tag="VBD27_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO7024416.1"
                     /translation="MTELIRILHLNDLHSHFENFPKVKRFFHNNQAQPIETISLDLGD
                     NIDKSHPLTEASSGKANVQLMNELGIELATIGNNEGVGLSKKDLDQVYKDSDFTVIVG
                     NLKDNIIEPSWAKPYIIYETQQGTKLAFLAYTFPYYKTYEPNGWTIEDPIDCLKCHLQ
                     INEIKEANCRILMSHLGIRFDTRIAQEFSEIDLIIGAHTHHLFEEGELITGTYLAAAG
                     KYGRFVGSIDITFDNHTLKDILISTYDTKQLTGYPSDSDWAKEIESEGKEQLRKESLI
                     SFPAPLSLEESCQLVMDAMLFYAGADVAIINSGLIVQPFEKDFSRKNLHESLPHQMRL
                     AKLTVSSQELLEIYETIYQQGQFLAQQKIHGMGFRGKYFGEVLHSGFDYKNGKIVYNE
                     KDIDAKEEVILVIVDQYYFASYFECLKTKKVDLLFPELLRDVVADYLINMTL"
     gene            11244..11822
                     /locus_tag="VBD27_000380"
     CDS             11244..11822
                     /locus_tag="VBD27_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEO7024417.1"
                     /translation="MRKEVTPEMLNYNKYPGPQFIHFENIVKSDDIEFQLVINEKSAF
                     DVTVFGQRFSEILLKYDFIVGDWGNEQLRLRGFYKDASTIRKNSRISRLEDYIKEYCN
                     FGCAYFVLENPNPRDIKFDDERPHKRRKSRSKSQSSKSQTRNNRSQSNANAHFTSKKR
                     KDTKRRQERHIKEEQDKEMTSAKQHFVIRKKK"
     gene            11873..12979
                     /gene="rlmN"
                     /locus_tag="VBD27_000381"
     CDS             11873..12979
                     /gene="rlmN"
                     /locus_tag="VBD27_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEO7024418.1"
                     /translation="MPKKTDTPDYKPSIYSLTRDELIAWAIEHGEKKFRASQIWDWLY
                     KKRVQSFDEMTNISKDFIALLNENFVVNPLKQRIVQESADGTVKYLFELPDGMLIETV
                     LMRQYYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEITAQIMLVQKYFDERGQG
                     ERVSHIVVMGIGEPFDNYTNVLKFLRTVNDDNGLAIGARHITVSTSGLAHKIREFANE
                     GVQVNLAVSLHAPNNELRSSIMRINRSFPLEKLFAAIEYYIETTNRRVTFEYIMLNGV
                     NDTPENAQELADLTKKIRKLSYVNLIPYNPVSEHDQYSRSPKERVEAFYDVLKKNGVN
                     CVVRQEHGTDIDAACGQLRSNTMKRDRQKAKVGR"
     gene            12979..13494
                     /locus_tag="VBD27_000382"
     CDS             12979..13494
                     /locus_tag="VBD27_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO7024419.1"
                     /translation="MLKRLFTEDGELTKISRRFVWMLVVIYCLIIVRMCFGPQIMIEG
                     VSTPNVQRFGRIVALLVPFNSFRSLDQLTSFKEIFWVIGQNVVNILLLFPLIIGLLSL
                     KPSLRKYKSVILLAFLMSLFIECTQVVLDILIDANRVFEIDDLWTNTLGGPFALWSYR
                     NIKGWLLTIRK"
     gene            13690..15435
                     /locus_tag="VBD27_000383"
     CDS             13690..15435
                     /locus_tag="VBD27_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7024420.1"
                     /translation="MSIIKNLWWFFKEEKKRYLIGILSLSLVAVLNLIPPKIMGSVID
                     AITTGKLTRPQLLWNLLGLVLSALAMYGLRYIWRMYILGTSYKLGQVVRYRLFEHFTK
                     MSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFTISWQM
                     TLIAVIPLPLMALATSKLGRKTHETFKESQAAFSELNNKVQESVSGVKVTKSFGYQEQ
                     EIASFQEINQMTFVKNMRTMTYDVMFDPLVLLFIGASYVLTLAMGAFMISKGQVTVGD
                     LVTFVTYLDMLVWPLMAIGFLFNMVQRGSVSYNRINSLLEQESDITDPLNPIKPVVNG
                     TLRYDIDFFRYDNEETLADIHFTLEKGQTLGLVGQTGSGKTSLIKLLLREHDVTQGKI
                     TLNKHDIRDYRLSELRQLIGYVPQDQFLFATSILENVRFGNPTLSINAVKEATKLAHV
                     YDDIEQMPAGFETLIGEKGVSLSGGQKQRIAMSRAMILDPDILILDDSLSAVDAKTEH
                     AIVENLKTNRQGKSTIISAHRLSAVVHADLILVMRDGRVIERGQHQELLNKGGWYAET
                     YASQQLEMEEAFDEV"
     gene            15425..17164
                     /locus_tag="VBD27_000384"
     CDS             15425..17164
                     /locus_tag="VBD27_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7024421.1"
                     /translation="MKSNQWQVFKRLISYLRPYKWFTVLALSLLLLTTVVKNIIPLIA
                     SHFIDHYLTNVNQTAVLILVGYYSMYVLQTLIQYFGNLFFARVSYSIVRDIRRDAFAN
                     MERLGMSYFDRTPAGSIVSRITNDTEAISDMFSGILSSFISAIFIFTVTLYTMLMLDI
                     KLTGLVALLLPVIFILVNVYRKKSVTVIAKTRSLLSDINSKLSESIEGIRIVQAFGQE
                     ERLKTEFEEINKEHVVYANRSMALDSLFLRPAMSLLKLLAYAVLMSYFGFTGVKGGLT
                     AGLMYAFIQYVNRLFDPLIEVTQNFSTLQTSMVSAGRVFDLIDETGFEPSQKNTEAFV
                     REGNIEFKNVSFSYDGKKQILDNVSFSVKKGETIAFVGATGSGKSSIINVFMRFYEFQ
                     SGQVLLDGKDIRDYSQEQLRKNIGLVLQDPFLYHGTIKSNIKMYQDITDQEVQDAAEF
                     VDADQFIQKLPDKYDSAVSERGSSFSTGQRQLLAFARTVASKPKILILDEATANIDSE
                     TEQIVQDSLAKMRQGRTTIAIAHRLSTIQDANCIYVLDRGKIIESGNHESLLDQKGTY
                     YRMYQLQAGMMEV"
     gene            17292..17858
                     /locus_tag="VBD27_000385"
     CDS             17292..17858
                     /locus_tag="VBD27_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEO7024422.1"
                     /translation="MLLLVDNYDSFTYNLKQYLSVYKEIFVIKNDDPNLFLLAESAEA
                     IVLSPGPGHPKDAGKILELINQFIGQKPILGICLGHQALAECLGGRLNLANHVMHGKQ
                     SWVTINDHTSLFKGIDSPTQVMRYHSLVVTDLPENIAIIARSNEDNEIMAFHCPSLKV
                     YAMQFHPESIGSIDGMKMIENFLTLIND"
     gene            complement(17926..18464)
                     /locus_tag="VBD27_000386"
                     /pseudo
     CDS             complement(17926..18464)
                     /locus_tag="VBD27_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857893.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
     gene            complement(18465..19457)
                     /gene="bioB"
                     /locus_tag="VBD27_000387"
     CDS             complement(18465..19457)
                     /gene="bioB"
                     /locus_tag="VBD27_000387"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEO7024423.1"
                     /translation="MSFQTNYIHLADEILSGKTSISYEQALEILNSDENWWEIYAAAL
                     YLKNQVSRNNIRLNVLLSAKQGLCAENCGYCSQSKESTADIDKFGLLPQNVILKQAIV
                     AHQNGASVFCIAMSGTKPSKREIEQLCQVIPEIKKSLPLEICLTAGFLDREQLHQLKQ
                     AGIDRINHNLNTPEENYPNIATTHSFKDRCDTLERIHNEDIDVCSGFICGMGESDEGL
                     ITLAFRLKELNPYSIPVNFLLAVEGTPLGKYNYLTPIKCLKIMAMLRFVFPFKELRLS
                     AGREVHFENFESLVTLLVDSTFLGNYLTEGGRNQHTDIEFLEKLQLNHTKKELI"
     gene            19582..20097
                     /locus_tag="VBD27_000388"
     CDS             19582..20097
                     /locus_tag="VBD27_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024424.1"
                     /translation="MKKLFKKAIAMCRDLPLNFVIVELSLYQREDGSSDNIKWISKRI
                     LEVTVTDQQWIYHLRMLNLPKLKPMSLASSDCPAREEKASLISIEECQKFTSLVRDKN
                     PFHQGEAAIVPAALLLEKIMEVRNGKIKEIHLTFLSPVRVNQEFSVQVVDNTVIIFQH
                     QILCVRGTYEE"
     gene            20087..21202
                     /locus_tag="VBD27_000389"
     CDS             20087..21202
                     /locus_tag="VBD27_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEO7024425.1"
                     /translation="MKNRDVYIGFGLRTPIGIKGKQFKHYRPELLGAHLLNQIKKIES
                     ESNIDSIICGNTVGTGGNIGRLMTLFSDYESYIPVQTIDMQCASSSSALFFGYLKISA
                     GINEKVLVGGIESSSLQPMRRYAKEDNRNGEYTVAQFSPDSYAETVMLEGAQRVCQKY
                     GFRREMLDKLAFLSHKRALTAKQGGYLEEVILPMEGMRDQGVRKLKEAFFQKLPRLME
                     NSPLLTIGNVCLMHDAAAFLTLQSQKTEFRIVHIVEVAGDPKLSPELVHTATEKLLTE
                     THTKISDYDAIEWNEPFAAIDALFNHYYPEEREKFNIFGGALAYGHPYACSGIINILH
                     LMQALKYKNKPMGLTAIAGAGGVGMAISIEYLGVKNA"
     gene            21195..22424
                     /locus_tag="VBD27_000390"
     CDS             21195..22424
                     /locus_tag="VBD27_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO7024426.1"
                     /translation="MLESLKTIVKTNSDKKLFDGDLQVSYGEFYNLVRQDMASQDNRK
                     HVISTHSLLNQLVRFVSKLCQKALPIICKPNLTHNEISRLEKEVQYAPQLADFGVLSS
                     GTTADAKLLWRSFASWSDFFSIQNAYFSVTSNSRLFIQGDFSFTGNLNLALSLLLLGG
                     TLVVTQKNSVKYWQTLWEKTGVTHLYLLPSYLKLVEQYSKETALDNKTIITSSQYVSD
                     SLLEGLYIKHPKVSVKIFYGASELNYVSWYDGRDIRDKPQYVGEIVPNVAVRIKEGRI
                     FVKTPYSICGLSSEYCAGDYGELIDGKLYLFGRGDDWCNQSGIKLYLPRLIEKIKTCP
                     YIKDAVAFTKESQSHGQESHCCIVLIENQMQQECLKWLSEHFEKKYGFKHYHIVSKIP
                     LMPSGKIDYQQLKRQLA"
     gene            22537..23169
                     /gene="nth"
                     /locus_tag="VBD27_000391"
     CDS             22537..23169
                     /gene="nth"
                     /locus_tag="VBD27_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO7024427.1"
                     /translation="MLSKAKSRYIIREIIKLFPDAKPSLDFTNVFELLVAVMLSAQTT
                     DAAVNKVTPALFERFPNPLVLAQADPKEIEPYISKIGLYRNKARFLNQCAKQLIEHFD
                     GKVPQTRQELESLSGVGRKTANVVMSVGFGIPAFAVDTHVTRICKHHQICKQSASPLE
                     IEKRVMEVLPPEEWLAAHQSMIYFGRAICHPKNPKCDQYPQLYHFPDNLK"
     gene            complement(23170..23529)
                     /locus_tag="VBD27_000392"
     CDS             complement(23170..23529)
                     /locus_tag="VBD27_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO7024428.1"
                     /translation="MSFILSYYDIKYQEYPIFLWILFTISTIILTPITKVSIVLCLFG
                     ILAEVVDINIGSGDFLYLATIGLSLPLHQMLFIIQIGAWLGIIYCLVMRKMKKTIAFL
                     PFLSIAYIIVTSYSLLF"
BASE COUNT         7655 a         3625 c         4703 g         7566 t
ORIGIN      
        1 tgaatagaca tagaaagtga agatacatga ggactttata aatagcagga aagtagatac
       61 tagttagata tagtagtgca agatagatga gaaaaaataa cgaatacttt aacttggtca
      121 taacaattcc attctaatgg tagtaagcat gcataaaatg taggttttat catactactt
      181 gaatccagca actagataga gggggttaca tacgataaga cagtagtatt ttaaaccgat
      241 cggttgactt attgatactg tttagggaac aagtgttttg ttgtcaatgt taaatttaat
      301 ttagcatttt gatggtatca ccgcaagaat gaaaggaaaa gttgtatgtg ctgaaaaaac
      361 taatgcttgg cgatttagaa cacacgttta ctagtcgaga tgacaaaaaa taagtaccaa
      421 atgtaaaggt cgatattatc agcattgtca gcgttaggtg ttataaaaat ttaacatctt
      481 ccaattaagc taggtttaag tttcctaagt tgaactacgc ctagccgaaa aataatttct
      541 tttttacaga actcctaaca aggattttga attgtcaaga ttcttgtttt tttggaaagt
      601 catttagagg ctttttgggg gaagtattaa ctattgtatc cgtttacaat tcctttcaaa
      661 aatgttagta tatcattgag ggatatgagt tatggataag ggagagagga tgatgatgac
      721 taaggaaacg ttggcacatt atcaaaagaa aatagaacaa gaatctgaaa aaaagcagtc
      781 tttagatgag cactcatggc atgtggcctg ttttagtcga caggaagcat ctataattgg
      841 tcaaggagat gtgctttttt taattggtct ataccacgat ctaggcaaag ctgatcgagc
      901 ctttcaagat aaattattaa ataatccaaa tcgacatgtt gatcattctt atgcaggggc
      961 aaaatactta tgctctatta ttgggcctca tctaaaaagt cgaggggttg ataaaggtga
     1021 gagaatgaca ttcaatgaaa tggtgggtta tgtcatctcg gctcatcatg ggatgtatga
     1081 tttttgctac tgttctgacg atgctgaata ttatagcttt aataaattta aaaaccgtat
     1141 caacagagac ttagatgatt atcactatca cgaagatatt aaagggtatg ctataaaatt
     1201 agaagaaaaa ttgtgtgatt acggctacaa agatttaagg gagcttattg ataaagcttt
     1261 tgataattac caacaagcta tgtcttcctt aaactggcaa gataatagtg agtgggatta
     1321 ttatcagtct tgtatggtga gactttactt gtcgctctta aaaaacgctg atattttgga
     1381 cacagtaaat gcctatggtc ttaaaataag tcctatggat aaaacagagc gatcctctct
     1441 aaaacattct tatttagcag ccattgaaca aaaatatgct agctttggac gaccaaacaa
     1501 tcagttgaat actattcgga cagaaattgc tgagcgtgtt aaagaaagag gtaaacggga
     1561 ttccaaaggg atctatcgct tagatttacc gacaggagct ggcaagacta atcttagtat
     1621 gcgctatgcg tttcaccaat tagttcatca agacaaatca agattttttt acataacgcc
     1681 ctttctttcg gttcttgagc aaaatgcttc cgaaattaga aaagttacgg gtgaccttgg
     1741 cgttctagaa caccattcca atatggttaa acaggctaat gaagatgatg acaaggacag
     1801 tttattgtca gcttatctta ttgatagctg ggatagtcaa gtagtcttga cttctatggt
     1861 tcaatttttc caaacgcttt ttaaaacaaa atcggctaat ttgagacgtt tttcaagttt
     1921 gattaatagt gttgtgattc tagatgaagt tcagtcactg cctattgaag tcaccacttt
     1981 gtttaattta acgatgaatt tttttaataa agttatggat acaaccatcg ttctttgtac
     2041 agcaacacaa cctgcttatg attcttcaga gattgaccat cgtatctgtt atggagggaa
     2101 tttgggagaa ttagctgaaa tagtggattg aaataagttt atacgttgct tcatcaccaa
     2161 gttttacgtc gcacccattg cgggtgcgtg gattgaaata taagttcagc aacttcacga
     2221 gaaaatgttc ctcgtcgcac cctttgcggg tgtgtagttt caactatttt aaatctttca
     2281 aattatgcta ttaaaattgt ctaactttta acagagatgc gattgatttg tattaatccc
     2341 taaaaagata aattgctagt tcttatgcta taataactct atcaatgtca aaggagtttt
     2401 tatgttaaag gtaagatatg gtgttgtgtc aacggcaaag gtggcgcctc gttttattga
     2461 aggagtccgc ttagcaggaa atggtgaagt tgtggctgta tcgagtcgaa ctcttgagtc
     2521 tgcgcaagct tttgccaaca aatatcatct cccaaaggct tacgataagt tagaggatat
     2581 gttagcagat gagtcgattg atgtgattta tgtggcaaca attaatcaag accattataa
     2641 agttgctaaa gcagctctgt tagcgggaaa acatgtgttg gtagaaaagc ctttcacatt
     2701 gacttacgac caagcaaatg aattattcac tttggcagaa agttgtaatc tgtttttgat
     2761 ggaagcacaa aaatcggtat ttattccgat gacacaagtg attaaaaaat tacttgcctc
     2821 tggtgaaata ggagaagtga tctccatctc atctacaaca gcctacccaa acattgacca
     2881 tgtaacatgg tttagagaac ttgagttagg tggaggaaca gtgcatttta tggcgcccta
     2941 tgccctgtct tatttacaat acctatttga tgcgactata actcatgcta gtgggactgc
     3001 gacttttcca aaaggtcaaa gcgatagtca atccaaacta ctcttgcaat tatcaaatgg
     3061 ggtcctcgta gatatcttct tgacaacacg tttaaattta ccgcatgaaa tgattattta
     3121 tggaactgaa ggtcgtctaa tcattccaca tttttggaaa accactcatg caaaattggt
     3181 gagaaatgat actagtgcgc gaacgattca agttgacatg gttagtgatt ttgagaaaga
     3241 agcttatcat gttagtcaaa tgattttaga agggcaaagg gtaagtcata tcatgacgcc
     3301 tcagttgacg ctgtcaggtg ttaagattat agaaggctta taccgttcat gggggaaatg
     3361 atgctggcta tctagcatat taataaggag tactagatgt tattttagta gtcctttttt
     3421 gttttgctct cataaggtta attagattta aaagggtttt ctacttgttt tttctcgatt
     3481 tttgtttcat aatgtagaca agtttagagt gaggtgcttc tatgttttcg agcgattttg
     3541 gatttgagat aattaatgtt gacgatctat ccttacaaga ggagaaattt ttaccttcag
     3601 aattgctggc ttatgcacgt gatgaaaacg agtcttcttt cgtacgtgat attgaggggc
     3661 atttgacact tgtttaccag cttttggata cgcaaggtca cgttgatgat gttcgtcatg
     3721 tgccgagagt gataccagtg actctatttt tgaaagaaga tggcttgttt gtattagcta
     3781 atcataaaaa tattatcctt gtaaaaaaag cacttaatcg cgttgaaaag gtagattcgc
     3841 cgaaacacct cttattatca ttagtgactg ctttctcaaa gcaatatttt gatgttttag
     3901 atactatttc tgaagaaaga gataaactaa tcaatgattt gcgtaaaaga ccaaacaaat
     3961 cgaacttggc aaggctggca aatttacaaa gtggaacagt acacttaatg atgggaacta
     4021 agcaaaattt tgaaatgctg accgatttac aaaacattga gcaagataaa gagaatacta
     4081 gaaatgaaaa gatgcaactt caagatgcta ttattgaagc aagacaatta tctaacatgt
     4141 gttccttaaa ttcacaggtt ttccaagaac tttcttcttc ttataataat gtcctcagca
     4201 ataaccttaa cgacaatgta actaccttaa caattatttc cattggtatt tccattattg
     4261 ctatggttac cagcttttat ggaatgaatg ttaagttgcc ttttgatagt gttgatgctg
     4321 tctgggtatt gattatttta attacgacta tcattactat tatgctctct attgttatgt
     4381 acatttatgt ccataaagag gataatcatc ccaaacataa atagagaatt aataagaggg
     4441 tgctatcaat tagtttagtt ttaagcatgg caacatgctt tttttgtaca aagaaactag
     4501 aattcatctt tgactttgag cttgctttct aactcttaaa acgacataat agaggataat
     4561 gtacagtgaa tagagaggtt actatgtcaa tagaatcaga ttttttccgg aaaaaacgtt
     4621 ttattttttc atcgcttgag gagtttggat ttatcaaatc tgaccaagag tatatctatt
     4681 gtcaaacatt tatggataat gactttaaag ctattatcac gatttcattg gatggaaaga
     4741 tagctggtaa ggttattgat tcggcacttg aagaagaata tttaccgctt cgagcagcaa
     4801 attataatgg ttcgtttgtt ggcgaagtac gatcggctta tatggctata ttaggtgata
     4861 tttcagattc ttgttgtaag gatttattat ttaccaaaga tcaaagtaat cgtttagcgg
     4921 agaagatagc tacaactttt gaagattcag ttgattatcc atttgcgaag cacccacaat
     4981 atgcttctta tcgtgtatca ggtaaatggt atgctcttct ttttccattg aagatgggga
     5041 agctagaaaa tgttccagct caactgtcag aagaagaggt tgaagttttg aatattaaag
     5101 tcaatcctca agatatggaa atactcttac aaaaagaagg tatttatcct tcttatcata
     5161 tgtctaaaaa gacttgggta tctatcgttt tagacaatac tttgtctgat atagaaattt
     5221 ttaaattggt atcagatagt cgtaagcttg tttctcccaa taaaaaatca aattctgagc
     5281 cagagttttg gattattcct gctaatccta aattttacga tattgataag gagtttgcag
     5341 agcatcaatt gattgattgg actcaaaaag gaaagattgc ggtgggagat tatgtgttta
     5401 tttatattac agcacctatt cgtgctttgc gctatgtctg tcaagtgata gaacgtgacc
     5461 ttaaaacaag tacttactta ggagcaagag atgtcaaagc ttatatgcga ctcaaactga
     5521 taaggcaatt tgaagattct acctatcctg tagcattttt agcagaacac ggcgttaaga
     5581 ctgtgagagg accacggcga atgaccaagg aattgcgaca agtcatggca aaaacaattc
     5641 tagagtaatg actttcagaa aaaaacttgt aaaaaagtca gaataattct acaattcctc
     5701 ttccattttg ttacttgaag gagtataata gctctaacat tttataattt aaggaggaac
     5761 atatgaagaa aagctttatt catcagcaac aggagatttc ttttgtcaaa aatactttta
     5821 cacaatattt gattgataag ttagaaatcg tcgaagtgca aggacctatt cttagtcagg
     5881 tcggggatgg tatgcaagat aatttatctg gtattgaaca tcctgtatcg gtgaaagtcc
     5941 ttaatatacc tgaagctgaa tttgaagtcg ttcactcatt ggctaaatgg aaacgtcata
     6001 ctttggcaag atttgggttt aatgagggag aaggtctttt cgttcatatg aaagctcttc
     6061 gtccagatga agattctctt gatccaacac actcagttta cgtagatcag tgggactggg
     6121 agaaggtcat tcctgatggt agacgtaatt tggactacct aaaagaaact gtggaaaaaa
     6181 tctacaaagc tatccgccta acagagcttg ctgtggaagc tcgttttgat attgaatcca
     6241 ttttaccaaa gcgtattacc tttattcata cagaagaatt ggtagaaaaa tacccagact
     6301 taagccctaa agagcgtgaa aacgccatcg ctaaggagta tggcgcagtc tttttgattg
     6361 gtattggtgg agagttagca gatggtaaac cacatgatgg tcgtgcaccg gactatgatg
     6421 actggacaac accatcagaa aatggtttta aaggtctcaa tggtgatatt ttagtatgga
     6481 acgaacagct tggaactgcc tttgaactat catcaatggg aattcgagtt gatgaagatg
     6541 ctcttaaacg tcaagttgtt ctcaccggtg atgaagatcg tttagagttt gagtggcata
     6601 agacgctatt acgtggtttc ttccctctga ctattggtgg gggaattggt caatcacgat
     6661 tagctatgtt cttgcttcgt aaaaaacata ttggagaagt acagtcaagt gtctggccaa
     6721 aagaagtcaa agataccttc gaaaatatat tataaaaaat cagccaagca gttttgtttg
     6781 gctgatttta acaataagtt tatttttggc ttaagaaagt ttggatttgt tgaggtgtag
     6841 cttggcttcc ttgaacaaga tatttagtca tttttcctcg ttgataatgt gctaaatttg
     6901 gaacagtggt aatatgatgc tttttgaaaa aactcttcaa tcgtttccct ttgtattttt
     6961 gactatcaag gtagtaaatt tttgaatgaa gtgtggcttg agattttaat aattttggta
     7021 agaaagctct acaatattgg caagttggac gtcctataaa aatagtcgct tcagattgtt
     7081 gacttttgct tactaaggcc ttatctgtga tggcgattaa gtcactattt gttatcatgt
     7141 gttggtattg ttgttgagaa atagtcatgg tgtcagcgct acttaagttt gattttagcg
     7201 ttgtaaggca taatatggct attaaagtta ataataattt tttaatcatt atgggaacct
     7261 cgtttctctt actgctagct tatcatatca aaaaagaagg aaaagagata ctatcttctc
     7321 tgtttgttat aagcaatttt tttgataact ctaccttatt ttttgttata atagtggtta
     7381 tgagagtagt agcaggaaca tttggagggc gtcctttaaa aacgcttgat ggaaaaacaa
     7441 ctcgtccaac aactgataag gttaaaggcg ctatttttaa tatgattggt cctttttttg
     7501 aaggtggtcg tgttttagac cttttttctg gcagtggtag cttagctatt gaggcgatct
     7561 caaggggaat ggaccaagct gtcctagttg aaaaagatag gcgtgcgcag gtcgttattc
     7621 aagaaaatat tgcaatgact aagagtccgg agcaatttca attattaaaa atagaagcca
     7681 accgtgcttt agaacaatta acgggacaat ttgatttggt cttgttggac ccgccatatg
     7741 ctaaggaaga aattgtaaag caaatccaaa tcatggatag caagggttta ttaggcgatg
     7801 atatcatgat tgtttgtgaa acggacaaat cagttgattt acctgaagaa attgcttctt
     7861 ttgggatatg gaaacagaaa atttatggca tttctaaagt aacggtctac gttcgttagg
     7921 aggctttggt atggattaca agttagtgat gattattctc atcttagtca tattgattta
     7981 tttgttgttg cgccttcact atttagaaga gttgctaaaa cattatcact tcaaaaatga
     8041 tgaaggagtt tgttcattat caagaacaaa aaatccattg tatctattaa tggcaatgat
     8101 tacttttttg ccaaatctta tcaattggat agctaacggt tttgaggcaa tagacattgt
     8161 tcgcctgatt cctgtggtag tctttttatg gttgtattat tgtggagatg acgacgaatg
     8221 acaaagaaag ctttatttac aggttcattt gatcctgtta ctaatggaca tttagatatt
     8281 attgagcgcg ccagctatct atttgatcat gtttacattg ggttatttca taaccttgag
     8341 aagcaaggtt acttttcgat tgagtgccgt aaaaaaatgt tagaagaagc tatccgtcag
     8401 tttaagaatg tttcagtttt agtggcgcaa gatagactag ctgttgactt agcaagggaa
     8461 gtgggagcaa aatattttgt tcgaggtctg cgtaatagcc aggattttga ttatgaagcc
     8521 aatttggaat tttttaataa acagttggca gatggtattg agacagttta tttatcaacg
     8581 tcgccatctt tgagtccgat atcatctagt cgcatccgtg aattgataca ttttaaggct
     8641 tctgtgaagc cttttgttcc aaagagtgtt tttagagaag tggagaaaat gagtgaagaa
     8701 taaagatcca aaaagaaaac ataaaagttt gctaggtctg cttaaatggt ggattatagg
     8761 ttttgctttt ttattgttag tattagcaag ccttgtggtg aggctacctt attatttgga
     8821 aatgccaggt ggagcttatg atatccgttc ggtattgaaa gtgaataaaa aggctgataa
     8881 agctaagggt tcgtacaatt ttgtagcggt atctgtcagt caagcaacgc cagctcaggt
     8941 tctttatgct tggttaactc cttttacaga attatctagt aaagaggaga caacaggtgg
     9001 ctttagtaat gacgattatc tcagaattaa tcaattctac atggagactt ctcaaaatga
     9061 gtccatctat caagctttaa agttagctaa taagcaagta tctcttactt ataaaggagt
     9121 ttatgtgctt aacttagcta aaaattcgac atttaaagat agattacatt tagctgatac
     9181 agtcacagga gttaatggca aaagtttcaa aaattcttct caacttatca aatatgttgc
     9241 agctcttcat ctaggtgata aagtaaaggt acaatacaca agtcaaggta aaaagaaaga
     9301 atcagttggt aaagttatta aactatcaaa cggtaaaaat ggtattggta tcggtttgac
     9361 agaccataca gaagtatcat ctgatgttcc tgtggacttt aatactgaag gtgttggagg
     9421 accaagtgca ggtttaatgt ttactttagc gatttacgac caattagtta aagaagattt
     9481 acgtaaagga cgtaagattg caggcactgg tactattgag caaaatgggc atgttggcga
     9541 tattggtgga gcaggtttga aagttgtttc agcggctaaa aaaggaatgg atattttctt
     9601 tgttcctaat aatccgatcg ataaaaatgc taaaaaaggc aaaacaaagg ttcagacgaa
     9661 ttatcaagag gcaaaagcag cagctaagcg attaggtacg aagatgaaaa ttgtaccagt
     9721 tcaaaatgtt cagcaagcta ttgattattt gaaaaaaaca aaataaactc aaagagttcg
     9781 ggcgtctgct cggctttttt gttttgcgac atttaactat caaagtgcta aaatgatagt
     9841 atgacagagc ttataagaat actacattta aatgatctgc actctcattt tgagaatttt
     9901 cctaaggtaa aacgattttt tcataacaat caagcgcagc caatagagac gatttcactt
     9961 gacttggggg ataatattga taaaagccat cctttgacag aagcaagctc gggcaaggct
    10021 aatgtccaat tgatgaatga actgggtatt gagcttgcta ctattggtaa taatgaagga
    10081 gttggtttat ccaaaaaaga cttggaccaa gtctacaaag attcagattt tacggttatt
    10141 gttggtaatc taaaagataa cataatcgaa ccgagttggg caaaacctta cattatttat
    10201 gagacacagc aaggaactaa gcttgctttt ttagcttata cttttcctta ttataaaacc
    10261 tatgaaccta atggatggac gatagaagat ccaattgact gtcttaaatg ccatttacaa
    10321 ataaatgaga taaaagaggc aaattgtcgt attttgatga gtcatttagg tattcgtttt
    10381 gatacaagga ttgctcaaga attttcagaa attgacctta ttataggtgc acatacacac
    10441 catttatttg aggaagggga gctaattacc ggtacatatc ttgcagcagc tggtaaatat
    10501 ggtcgctttg ttgggagtat tgatataact tttgataatc atacactaaa agatatttta
    10561 atctcaacat atgatacaaa acaactaact ggttatcctt cggacagtga ttgggctaag
    10621 gagattgagt cagaaggtaa agaacagctt agaaaagaaa gtttaatatc ctttccagca
    10681 ccattatcat tagaagaatc gtgtcaacta gtcatggatg cgatgctgtt ttacgcaggt
    10741 gcagatgttg ctattatcaa ttctggctta attgttcagc catttgaaaa agacttttcg
    10801 aggaaaaatc tgcatgaatc attacctcat caaatgcgtc tcgctaaatt gacagtgagt
    10861 agtcaagaac ttttagagat ttatgaaact atttatcaac aaggtcaatt tttagcgcaa
    10921 caaaagattc atggtatggg ctttcgtggc aagtactttg gagaagtttt acattcggga
    10981 tttgattaca aaaatggaaa aatagtgtat aatgagaaag atattgatgc caaagaagaa
    11041 gtgattcttg taatcgttga tcagtattat tttgcatcgt attttgagtg tttaaaaaca
    11101 aaaaaagtag acttgctttt tcctgaatta ctcagagatg tggtagcaga ttatttaata
    11161 aatatgacac tataaatgaa aaatagataa ataaagagta gaagctagtc gtttctctca
    11221 atgattttag agaaaggagt attatgcgaa aagaagtgac accagagatg cttaactata
    11281 ataagtatcc tggcccacag tttattcact ttgaaaatat cgttaaaagt gatgatattg
    11341 aatttcaact tgttattaat gaaaaatcag cttttgatgt gactgtcttt ggacaacgtt
    11401 tttctgagat tttattaaaa tatgatttta tcgttggcga ttggggtaac gagcagttga
    11461 ggctaagagg cttttacaaa gatgctagta cgattagaaa aaatagccgg atttcacgtt
    11521 tagaagatta tattaaagag tattgtaact ttggttgtgc ttattttgtg ttggagaatc
    11581 caaatcctag agatattaaa tttgatgatg aaagacctca taagcgtcgt aagtcaagat
    11641 ccaaatcaca atcatcaaag tcacaaacta gaaataatcg ttcccagtca aatgccaatg
    11701 ctcattttac aagtaaaaag cgtaaagaca caaaacgccg tcaagaacgt catattaaag
    11761 aagagcaaga taaggaaatg acctctgcaa agcagcattt tgttattcgt aagaaaaaat
    11821 aaaaaaagga taaactgtaa tcgtttattc caccatgaag aaagaaaata ttatgcctaa
    11881 aaaaactgat acacccgact ataaaccctc tatttatagc ttaactcgcg atgaactaat
    11941 agcttgggct attgaacatg gagaaaagaa attccgtgct tcacaaatct gggactggct
    12001 ctataaaaag cgtgtgcaaa gcttcgatga aatgactaac atttccaaag attttattgc
    12061 acttttgaat gagaattttg ttgtcaatcc tctaaaacaa cgtattgttc aagaatctgc
    12121 tgatggaact gttaaatacc tttttgaatt acctgatggc atgctgattg agacggtttt
    12181 gatgcgtcaa tattatggtt tatcagtatg tgtaacgact caagttggtt gtaacattgg
    12241 ttgcactttc tgtgcaagtg gtttaataaa gaaacaacgt gatcttaaca atggcgaaat
    12301 cactgctcaa attatgcttg ttcaaaaata ttttgatgaa cgtggacaag gtgagcgcgt
    12361 tagtcacatt gtggtcatgg gaataggtga accctttgat aactatacca atgtattaaa
    12421 attcttgcgt acagttaatg atgataatgg attagctatt ggtgcacgtc acattacagt
    12481 gtctacttca ggtttagcac ataaaattcg tgaatttgca aatgaaggtg tacaagttaa
    12541 cttagcagta tctcttcatg ctccaaataa tgaattacga tcaagtatca tgcgtattaa
    12601 tcgttctttc cctcttgaaa agttgtttgc ggctattgaa tattatattg agacgactaa
    12661 ccgtcgtgta acatttgagt atattatgtt aaatggtgtc aacgataccc cagaaaatgc
    12721 tcaagagtta gcagatttaa cgaaaaagat tcgcaaatta tcttatgtca atttaattcc
    12781 ttacaatcct gtctcagaac atgaccagta tagtcgtagt cctaaagagc gtgtggaagc
    12841 tttctatgac gttttgaaga aaaatggagt taattgtgtt gttcgtcaag aacatggtac
    12901 ggacattgat gcagcttgcg gacaattacg ttcaaacaca atgaaacgcg atcgtcaaaa
    12961 agcgaaggta gggcggtaat gcttaaaaga ttatttactg aagatgggga attgacaaag
    13021 attagtcgtc gtttcgtttg gatgttagtg gttatctatt gtcttattat tgtcaggatg
    13081 tgttttgggc ctcaaattat gattgagggg gtatcaactc cgaatgttca gcgcttcgga
    13141 agaattgtag ctcttttagt accatttaat tcttttcgta gtttagatca gctaactagc
    13201 tttaaagaga ttttttgggt tattggtcaa aatgtagtga atattttact gctgtttcct
    13261 ctcattatag ggttactatc cctaaagcca agtttacgga aatataaaag cgttatatta
    13321 cttgctttct tgatgtctct tttcatagag tgtactcaag ttgttttaga tattttaata
    13381 gatgctaatc gggtttttga aatcgacgat ctatggacaa ataccttagg cggtcctttc
    13441 gccctatgga gttatcgaaa cataaaaggt tggcttctaa ctattagaaa atgaaaataa
    13501 tgtaaaggct atgatattgt tcaagccttt tactatttaa aaaggatcac tttagttgac
    13561 gaaagtaagc ttatagaggc ataatagaga gttgttaaat gagaatttta aaacactttt
    13621 tatgtcttct gattgatttg gtcttctgac ttaattatga tgtttttcaa cctttgagaa
    13681 aggaaaaata tgtctattat aaaaaatttg tggtggtttt ttaaggaaga aaagaagcgc
    13741 tatcttattg ggattttgtc attaagtttg gttgcagtgt taaatcttat ccctcctaaa
    13801 atcatgggat cagttattga tgctattaca actggaaaat taacaagacc acaattacta
    13861 tggaatttat taggtttggt tttgtcagct ttagctatgt atgggctgcg ttatatttgg
    13921 cgtatgtata ttttagggac ttcttacaaa ttaggccaag ttgtcagata ccgtttattt
    13981 gaacatttta caaaaatgtc tccttctttt tatcagaaat atcgtacagg tgatttaatg
    14041 gcgcacgcga ccaacgacat caattctcta acacgtcttg caggaggagg agttatgtca
    14101 gcagtggatg cctctatcac agcattagta acgcttatca ccatgttctt tactatttcg
    14161 tggcaaatga cattaattgc ggttatccct ttgcccttaa tggccttagc aactagtaaa
    14221 ttggggcgaa aaacccatga aaccttcaaa gaatctcagg cagccttttc agaattaaat
    14281 aataaagtgc aagaaagtgt ctctggcgtc aaagtgacta aatcatttgg ttatcaagaa
    14341 caagaaattg cttcttttca agagattaat cagatgactt tcgtgaagaa catgcggacc
    14401 atgacttatg atgtcatgtt tgatccttta gttcttcttt ttataggtgc ctcctacgta
    14461 ttaacattgg ctatgggagc ttttatgatt tcaaaaggtc aagttactgt tggtgacttg
    14521 gtaacatttg tgacgtattt agatatgttg gtatggccct tgatggcgat tggtttcttg
    14581 ttcaatatgg tacagcgtgg tagtgtttct tataaccgta ttaatagtct acttgagcaa
    14641 gaatcggata taactgatcc tttaaatcct atcaaacctg ttgtcaatgg aacattaaga
    14701 tatgatattg atttctttag atacgacaat gaggaaacct tagccgatat tcatttcacc
    14761 ttagaaaaag gtcaaacctt aggtttggta ggtcaaacgg gatcagggaa gacaagtctt
    14821 attaagttat tgctacgtga acatgatgtg actcagggga aaattacttt aaataaacat
    14881 gatatacgtg attatcgatt gtctgagtta cgtcaactaa tcggttatgt tcctcaagat
    14941 cagtttttat ttgctaccag tattttagaa aatgttcgct ttggaaatcc aactctatct
    15001 atcaatgctg tcaaagaagc aactaaattg gcacatgttt acgatgacat tgaacagatg
    15061 ccagcaggat ttgagactct aattggagaa aaaggagtct cattatctgg tggacaaaaa
    15121 caaaggattg cgatgagtcg tgccatgatt ttagatccag atattcttat tttggatgat
    15181 tctctatcag cagtggacgc taaaacggaa catgctattg ttgagaatct taaaacgaat
    15241 cgtcaaggga aatcgactat tatttcagca catcgtttat cagctgttgt gcacgcagac
    15301 cttatcttag ttatgcgaga cggcagagtc attgagcgag gtcaacatca agagttgcta
    15361 aataaaggtg gttggtatgc tgaaacgtat gcctcacagc aattagaaat ggaggaagca
    15421 tttgatgaag tctaatcaat ggcaagtctt taagagatta atctcctatt tacgccctta
    15481 taaatggttt acagtattag ctctatctct cttattgttg acgactgttg ttaaaaatat
    15541 tattccttta attgcttcac attttattga tcactatctg acaaatgtta atcaaacagc
    15601 agttcttatt ttagtgggat attattcaat gtatgtcttg cagaccttaa ttcaatattt
    15661 tgggaatctc ttttttgcgc gtgtttctta tagtattgtt agagatattc gtagagatgc
    15721 ttttgctaat atggaaaggc taggcatgtc ttattttgat aggacaccgg caggatctat
    15781 tgtgtcacgt attactaatg atactgaagc aatatctgat atgttttcgg gtattttatc
    15841 aagttttatc tcggcgatat ttatttttac agttactctg tacactatgt tgatgctaga
    15901 cattaaacta acaggactcg tcgctctttt gttacctgtt atctttatat tagtgaatgt
    15961 ctatcggaaa aaatcagtca ctgtcattgc taaaacgaga agtttactta gtgatatcaa
    16021 cagtaaatta tcagaaagta ttgaaggaat tcgcattgta caggcttttg gtcaagaaga
    16081 gcgcttgaag actgaatttg aggaaattaa caaagagcat gttgtgtatg ccaatcgttc
    16141 tatggctctt gatagtctct tcttaagacc ggcgatgtct cttttaaaac tcctagcata
    16201 tgctgttctt atgtcttatt ttggatttac aggagttaaa ggaggtctta cggcaggatt
    16261 aatgtatgct tttattcagt acgttaatcg tctatttgac cctttaattg aagtaacgca
    16321 aaatttttca accttacaaa catcaatggt atcagcaggg cgtgtgtttg atctgattga
    16381 tgaaacaggt tttgaaccaa gccaaaaaaa tacagaagct tttgtgagag aaggaaatat
    16441 cgaatttaaa aatgtatcat tctcatatga tggaaaaaaa caaatccttg ataatgtttc
    16501 ttttagtgtt aaaaaaggtg aaactattgc ttttgtagga gcgacaggtt ctggaaaatc
    16561 atctattatt aatgtcttta tgaggtttta cgaatttcaa tctggacaag ttttattaga
    16621 tgggaaggat attagggatt actcacaaga acaattgcga aagaatattg gactcgtctt
    16681 acaagacccc tttttatatc atggcactat taaatctaat atcaaaatgt accaagacat
    16741 caccgatcaa gaggtacagg acgcagcaga gtttgttgat gctgatcaat ttattcagaa
    16801 gttgccagat aagtacgatt cagctgtttc agaacgtggg tcaagttttt caactggaca
    16861 acgccaacta ttagcgtttg ccagaactgt ggctagtaaa cctaaaattt taatcttaga
    16921 tgaggcgaca gcaaatattg attcggaaac agagcagatt gtacaagatt cgttagcaaa
    16981 aatgcgtcaa ggtcgaacaa caattgccat tgctcaccgc ttatcaacca ttcaagatgc
    17041 taattgtatc tatgttcttg atagaggaaa gataattgaa agtgggaatc acgagtcttt
    17101 attagaccaa aaagggactt attatcgtat gtaccaatta caagccggca tgatggaggt
    17161 ctagaaaatt taggctattc aaaaggcata atctgtgagg cttagaggtt ggaatagtta
    17221 ttcctctaag ccttttttat attttacaat tagtgtataa tgaggtcaaa gtacaaggaa
    17281 aggtctataa aatgttacta ttggtagata attacgattc ttttacctat aatttaaagc
    17341 aatatttaag tgtctataaa gaaatattcg tcataaaaaa tgatgatcct aacctttttt
    17401 tattagctga gagcgcagaa gctatcgtct tatcacctgg accaggacac ccaaaggatg
    17461 ctgggaaaat attagaatta atcaatcagt ttataggaca gaaacctatt ctaggtatct
    17521 gtttggggca tcaagcgctt gctgagtgct tgggtgggcg attaaattta gcaaatcacg
    17581 ttatgcatgg taaacaaagt tgggtaacaa taaacgacca tactagtttg tttaagggaa
    17641 ttgatagtcc aacccaagtt atgcgttatc actcattggt agtgactgat ttaccagaga
    17701 atattgctat tattgcacga tcaaatgagg ataatgaaat catggcattc cattgcccca
    17761 gcttaaaggt atatgcgatg cagtttcatc ctgagtcaat cgggagtatt gatggaatga
    17821 aaatgattga aaactttttg acgttaatta atgactaaaa tgtgttagtg taggataatg
    17881 actctctata aataaaagac agtacctaag ggcactgtct tttatttagt tgtatagatg
    17941 agttaatctg tctgcaaatt tacgataaat aatcaagcaa atggatgctt ttaaggtatc
    18001 cccaggaata aaaataaggt tagaaaatag tgatttagtt aaggggagag aagtttgaaa
    18061 ggaaagaacg atgctaccac aaatatcaat caatagtacg ccaaatatcc aaataataag
    18121 gaattaaacc cagagttttg ttgttttcac tttctcaagc cctaggccga taaaaaaagc
    18181 tgcaaatgga taagtcagca gatagcctgc tgttggccca aacagcgtta cgagacctga
    18241 ccgccctcct ggtaaaatgg tgcaccaata gcaactaaga gcaagaaaat agccacagct
    18301 aggaaacctt tcctactacc aagcagagca cctgccagca taacaccaag attttgtaag
    18361 acaattggga ctggaatgaa acctaatggg atccctggga taaaacctaa aacaatgagt
    18421 agtgcaacca tgagtgcaat ataggtagtt gttcttgttt tcatttaaat taattccttt
    18481 ttagtatgat ttagttgtaa tttttccaag aattcaatat cggtatgttg attgcgaccc
    18541 ccctctgtta ggtaatttcc caaaaaagtt gagtcaacaa gtaaggtgac taatgattca
    18601 aaattctcaa aatggacctc ccgtccagca cttaatctta attccttgaa aggaaaaaca
    18661 aaacgcaaca tggccataat ttttaagcat ttaatgggag tcaaatagtt atattttcca
    18721 agaggtgttc cttcaacagc aagtaaaaaa ttgacaggga tagaataggg gttcagttct
    18781 tttagtctga aagctaatgt gatgagcccc tcatcgctct ctcccatacc acaaatgaat
    18841 ccagaacaaa catcaatgtc ttcattgtga attctttcta aagtatcaca acgatctttg
    18901 aagctatgcg tcgttgcaat gttggggtag ttttcttcgg gggtgttgag gttatgattg
    18961 atacggtcaa ttcctgcttg ttttagctga tgaagttgtt ctctatccaa aaaaccagcg
    19021 gtaaggcata tttctagagg aaggcttttt ttgatttcag ggatgacttg acataactgc
    19081 tcaatttctc tcttgctagg cttagttcca ctcatcgcaa tacaaaagac gctagcacca
    19141 ttttgatgag cgacaatagc ctgttttaga atgacatttt gaggaagcag accaaattta
    19201 tcaatgtcag cagtgctttc tttagattga gaacagtaac cacaattttc tgcacaaagt
    19261 ccttgcttag cacttaataa aacgttaagg cgaatattat ttcggctaac ttgattttta
    19321 aggtataagg cagcagcgta gatttcccac cagttctcat cgctatttag aatttccagc
    19381 gcttgttcat aagaaatgct agtctttcca gataatattt cgtcagccaa atggatataa
    19441 tttgtttgaa atgacatgat agcctccttt aacgaaatta ataatagcat aaaaaaactg
    19501 tattgtaaac ttagtctata taataagtta acaatataaa aaattatgat atactggtct
    19561 tatattttga cgggagataa gatgaaaaaa ctatttaaaa aagcgatagc tatgtgccga
    19621 gatttgccgc ttaattttgt aattgttgaa cttagtttgt atcaaagaga ggatggctca
    19681 tctgataata ttaaatggat ttctaagcga atccttgagg taacggtaac tgatcagcaa
    19741 tggatttacc atttgaggat gcttaattta ccaaaactta agccaatgtc actagcatcc
    19801 agtgactgtc ctgcaaggga agagaaggct agtcttattt caatagaaga atgtcaaaaa
    19861 ttcactagct tagtgcgaga taaaaatcct tttcaccaag gggaagcagc tattgtgcct
    19921 gcggctctat tattggaaaa aataatggaa gttagaaatg gaaaaataaa ggagattcat
    19981 ttgacttttt tatctccagt aagggtgaat caagaattct cagtgcaagt agtggataac
    20041 acagtgatta tttttcagca tcaaatatta tgtgtgaggg ggacatatga agaatagaga
    20101 tgtttatatt ggttttgggc tacgaacacc tatcggtata aaagggaagc aatttaaaca
    20161 ttaccgtcca gaacttttag gagcacacct cttaaatcaa ataaaaaaaa tagaatcaga
    20221 atctaatatt gatagtatta tttgtgggaa cacagttggt actgggggca atattggtcg
    20281 tttgatgact cttttttctg attatgaatc ctatattcca gtacaaacga ttgatatgca
    20341 gtgtgcttca tcaagttctg ccttgttttt tggttatcta aaaatcagtg ccggtattaa
    20401 tgaaaaagtt cttgttgggg ggattgaaag tagttctctt caacctatga gacgttacgc
    20461 taaagaagat aatcgtaacg gagaatatac cgttgctcag ttttctcctg actcttatgc
    20521 tgaaactgta atgttagaag gggcacaaag agtctgtcaa aaatatggtt ttagaagaga
    20581 aatgttagat aaattggcat tcttgagcca taaacgcgcc ttaacagcta aacaaggtgg
    20641 ctatttagaa gaggtaatct taccaatgga agggatgcga gatcaaggcg ttagaaaact
    20701 aaaagaagca ttttttcaaa aattaccaag attgatggaa aattcacctt tgctcactat
    20761 tggaaatgtt tgtttaatgc acgatgctgc tgcatttcta acgcttcaga gtcagaaaac
    20821 agaattccgg attgttcaca ttgtagaagt agcaggagat cccaagctta gtccagaatt
    20881 ggttcacacg gctacggaaa aactattaac agaaactcat actaaaatat cggattatga
    20941 tgcaattgaa tggaatgaac catttgcagc gattgatgct ttatttaatc attattatcc
    21001 tgaagagaga gaaaaattca atatttttgg aggggcatta gcttacggac acccttatgc
    21061 ctgctcagga attattaata tccttcatct tatgcaggca ttaaaatata aaaataaacc
    21121 tatgggtcta actgccattg caggggcagg aggagtcgga atggccatat caatagagta
    21181 cttaggagtt aagaatgctt gaatcattga aaacaatagt taaaaccaat agcgataaaa
    21241 agttatttga tggtgattta caggtcagtt atggagaatt ttataactta gtcagacaag
    21301 atatggcgtc acaagacaat aggaaacatg tgattagcac acactctctg cttaaccaat
    21361 tagtaagatt tgtttcaaag ttatgtcaaa aagctcttcc tattatttgt aaacctaacc
    21421 ttactcataa tgaaataagt cgattagaaa aagaagtgca gtatgctcct caactagctg
    21481 attttggtgt tctttcttct gggactacag ctgatgctaa actattgtgg aggagcttcg
    21541 cttcgtggtc tgattttttt agcattcaga atgcttattt ttcggtcact tccaatagca
    21601 gactgtttat tcaaggtgat ttttcattta ctggtaactt aaatttagcg ctatcccttt
    21661 tattactggg gggaacgctt gttgtcacac agaaaaattc tgtaaaatac tggcagactt
    21721 tatgggaaaa aactggtgta acgcatctat atctgctccc tagttatctc aagttagtgg
    21781 aacaatatag caaggagact gcgttagaca ataaaactat tattacctct tcgcaatatg
    21841 tctctgactc attactggaa ggtttatata taaagcatcc taaagtgtca gtgaaaatct
    21901 tctatggtgc tagtgaactc aattatgtgt catggtacga tggtcgtgat atccgagata
    21961 agccacagta tgttggagaa atcgttccaa atgtagctgt taggattaaa gagggaagaa
    22021 tttttgtaaa aacaccgtat agtatctgtg gcctttcaag tgaatattgt gctggtgatt
    22081 atggtgaact gatagatggc aaattatatc tatttggtcg tggtgacgat tggtgcaatc
    22141 aatcgggcat taagctctat ttacctagat taattgaaaa aataaaaaca tgcccttata
    22201 tcaaggatgc agtagcattt acaaaagaga gtcaaagtca tggacaagag tcacattgct
    22261 gtattgtttt aattgaaaat cagatgcaac aagagtgttt gaagtggtta tcggaacatt
    22321 ttgaaaagaa gtatgggttt aagcattatc atattgtatc taaaattcct ttgatgccat
    22381 ctggaaaaat tgattatcaa caactcaaaa gacaattagc atgagttttt actaaaaaaa
    22441 taaaataggg ttaatggatt gcaaacctca gttctgatat tctttatttt gaaagatatc
    22501 gtgatataat gataaaaaag aaagataagg aataaaatgt tatcaaaagc aaaatcacga
    22561 tatattatcc gtgagattat taagttattt ccagatgcta aacctagctt agactttact
    22621 aatgtctttg agcttttggt agctgtaatg ctatcagctc aaacaactga tgcagctgtt
    22681 aataaagtca ctcccgccct ttttgaacgc tttccaaatc ctttagtttt ggcacaagct
    22741 gatcccaaag agattgaacc ctatatttct aaaattgggc tttaccgtaa taaagctcga
    22801 ttcttaaatc aatgcgctaa acaattgata gagcattttg atgggaaagt cccccaaact
    22861 cgtcaagagc tagaaagttt gtctggcgtt ggaaggaaaa cggctaatgt ggtcatgagt
    22921 gttggatttg gtattccggc ttttgccgta gatactcatg tcactcggat ttgtaaacac
    22981 caccagattt gcaaacagtc ggcttctcca ctagaaattg agaaacgagt catggaggtt
    23041 ttaccgccag aagagtggct agctgctcat caatccatga tttattttgg tcgtgcaatt
    23101 tgtcatccta aaaatccaaa atgcgaccag tacccacaac tttatcactt tcctgataat
    23161 ttaaagtgat tagaataaaa gagagtaact ggtgacgatt atataagcga tacttaaaaa
    23221 cggtagaaaa gctatcgttt ttttcatttt tctcataact aaacaataaa taattcctaa
    23281 ccaagcacct atttgaataa taaaaagcat ttgatgaaga ggtaaagata agccaattgt
    23341 ggctaaataa aggaaatctc cacttccgat attaatatca accacttcag ctaaaatccc
    23401 aaataaacat aaaacaatag aaacttttgt aatcggtgtg agaattatcg ttgaaatggt
    23461 gaataatatc catagaaaaa taggatactc ttggtatttt atatcataat aacttaagat
    23521 aaaactcatg cataaagaaa taaaataga
//
LOCUS       DATRQA010000013        23270 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.013, whole genome shotgun sequence.
ACCESSION   DATRQA010000013 DATRQA010000000
VERSION     DATRQA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23270
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.013"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            68..877
                     /locus_tag="VBD27_000393"
     CDS             68..877
                     /locus_tag="VBD27_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7024429.1"
                     /translation="MALILLFTADFLTYIIIELIISLDFWKLEWDSDIVQLIILLATS
                     ISTVMLFMWVKFKEKRPISSLGLFKEGALTELSRGWLVGSVLFGACLILTIISGSGVI
                     ETVRFTADKLIWFLIFAVGWQVQSASVEILCRGWLLPVSATKYSKIVSVSISSIFFGL
                     LHSANNHVSLISIFNLCLFGLFLSLYVILKGNIWGACGIHGAWNCVQGSVFGIEVSGE
                     PMLSNSLVHVKTYGADWISGGKFGVEGSMITSIVLIVACYWLYQKSNFPYD"
     gene            984..1574
                     /locus_tag="VBD27_000394"
     CDS             984..1574
                     /locus_tag="VBD27_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO7024430.1"
                     /translation="MPQNPTSNPEKKTYLLSELVDCFKGKAVPSKAEAGDIRIINLSD
                     MSPLGIDYHNLRTFQDEQRSLLKYLLQEGDVLIASKGTVKKVAIFEEQDYPVVASANI
                     TILRPTQHIRGYYLKLFFDSEEGQQALENANKGKAVMNISTKELLNIAIPSIPLFRQD
                     YLIQRYKQGLNDYKRKIARAEQEWERIQNDIRQQLS"
     gene            1608..2879
                     /locus_tag="VBD27_000395"
     CDS             1608..2879
                     /locus_tag="VBD27_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEO7024431.1"
                     /translation="MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKER
                     LILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFLIEKDNLLKDNEYQLAEQLNQKDMML
                     RDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKSDYEVQL
                     KAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGS
                     KGDFIYREKDENDLEFLSIMFEMKNESDDTIKKHKNEDFFKELDKDRREKSCEYAVLV
                     TMLEADNDYYNTGIVDVSHKYPKMYVIRPQFFIQLIGILRNAALNTLKYKQELALMKE
                     QNIDITHFEEDLDIFKNAFAKNYNSASKNFQKAIDEIDKSIKRMEAVKAALTTSENQL
                     RLANNKLDDVSVKKLTRKNPTMKAKFDALKD"
     gene            2892..3323
                     /locus_tag="VBD27_000396"
     CDS             2892..3323
                     /locus_tag="VBD27_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7024432.1"
                     /translation="MKIRTATLDDSEKLVPLYQELGYAISLSEIQSILKVILTHSDYG
                     FLIAEDNGKLLAFVGYHKLYFFEKSGTYYRILALVVNEKHRRKGIASQLINHVKQLAK
                     TDGSEVLALNSSLKEYRQEAYHFYENLGFKKVSTGFSYYLD"
     gene            3415..4299
                     /gene="truB"
                     /locus_tag="VBD27_000397"
     CDS             3415..4299
                     /gene="truB"
                     /locus_tag="VBD27_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO7024433.1"
                     /translation="MITGIINLKKEAGMTSHDAVFKLRKILHTKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTESGKIYEGEITLGYATSTEDSSGEVISRTPLTQSDLSEDVV
                     DHAMKSFTGPITQVPPMYSAVKVNGKKLYEYARSGEEVERPKRQITISEFRRTSPLYF
                     EKGICRFSFYVSCSKGTYVRTLAVDLGIKLGYASHMSFLKRTSSAGLSITQSLTLEEI
                     NEKYKQEDFSFLLPIEYGVLDLPKVNLTEEDKVEISYGRRILLENEADTLAAFYENRV
                     IAILEKRGNEFKPHKVLL"
     gene            4312..5244
                     /locus_tag="VBD27_000398"
     CDS             4312..5244
                     /locus_tag="VBD27_000398"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEO7024434.1"
                     /translation="MKIHYINDYKDIQAKEDCVLVLGYFDGLHLGHKALFDKAKKIAT
                     EKNLKIVVLTFNETPRLTFARFQPELLLHLTSPEKRSEKFQEYGVDELYLMNFTSHFS
                     KVSSDLFIKKYIYGLRAKAAVVGFDYKFGHNRTSGDYLARNFKGPVYIIDEISEGGEK
                     ISSTRIRQLITEGNVEKANQLLGYEFSTCGMVVHGDARGRTIGFPTANLAPINRTYLP
                     ADGVYISNVLINGKYYRAMTSIGKNITFGGTELRLEANIFDFDGDIYGETIEIFWLKR
                     IREMVKFNGIDDLVKQLKKDKEIALNWKKDSQTL"
     gene            5287..5700
                     /locus_tag="VBD27_000399"
     CDS             5287..5700
                     /locus_tag="VBD27_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO7024435.1"
                     /translation="MITLFLSPSCTSCRKARAWLSKHEVAFEEHNIITSPLNKEELLQ
                     ILSFTENGTEDIISTRSKVFQKLAIDVDELSTSSLMELISENPSLLRRPIILDKKRMQ
                     IGFNEDEIRAFLPRDYRKQELKQATIRAEIEGKHD"
     gene            5693..5974
                     /locus_tag="VBD27_000400"
     CDS             5693..5974
                     /locus_tag="VBD27_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEO7024436.1"
                     /translation="MISSNYSYPLDPSWNTEDITKVLRFLNQVEHAYENSVKVDDLLD
                     SYKEFKKFVKSKAQEKQIDREFQRTSGYSTYQAVKAAQQQAKGFISLGR"
     gene            5964..6728
                     /locus_tag="VBD27_000401"
     CDS             5964..6728
                     /locus_tag="VBD27_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO7024437.1"
                     /translation="MDAKFDFAKQLVYKAGQFIKSEMQNTFDVEEKSRFDDLVTSLDK
                     KTQKLLIQEIIQHYPDDNILAEEDDVRSPIAQGNVWVLDPIDGTVNFIVQKDNFAVML
                     AYYEEGVGQFGIIYDVMADILYSGGGHFDVYANDKKIVPFQECPLERCLLGVNSAMYA
                     ENDCGIAHLASETLGVRIYGGAGISMAKVMQGKLLAYFSYIQPWDYAAAKIMGETLGF
                     TLLTLDGEEPNYSTRQKVMFLPKSKLNLIQSYLTKK"
     gene            6796..8106
                     /locus_tag="VBD27_000402"
     CDS             6796..8106
                     /locus_tag="VBD27_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEO7024438.1"
                     /translation="MKLPNEFIEKYQTILKDEAEAFFDSFEQKPISAYRTNPLKEKQL
                     DFPNAIPSTPWGHYGKISGKSIEHTTGLVYSQEPAAQIVAQIAEPQEGMKVLDLAAAP
                     GGKTTHLLSYLNNTGLLVSNEISNKRSKILVENVERFGARNVIVTNESSQRLAKCFNS
                     FFDLIVFDGPCSGEGMFRKDPQAIQYWHKDYPTECAQLQRDILKEAIKMLAHGGILVY
                     STCTWSPEENEEVVNWLLQEYDYLELVDIPKLNGMVEGINVPQVARMYPHHFQGEGQF
                     VAKLRDTRSKEAQKIKPKAQKINKMQLQLWQQFAQDHLKIDLNGVLDVFGDQLYLLPN
                     GLPDLSKLKIARNGLHLGTFKKNRFEPSFALGMALSEHDLVQSIEIDIEQFEVYVSGN
                     VVKLDKTVPNGWYQILVKGNGLGFAKVTNNTLKNYYPKGLRFQT"
     gene            8285..9151
                     /locus_tag="VBD27_000403"
     CDS             8285..9151
                     /locus_tag="VBD27_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEO7024439.1"
                     /translation="MKMKKNIFLLTLLATVLLTLSGCANWIDKGQSITSVGSTALQPL
                     VEAAADEFGKTNLGKTINVQGGGSGTGLSQVQSGAVQIGNSDLFAEEKDGIDAKKLKD
                     HQVAVAGLAVIVNKKVNVKNLTTHQLRDIFAGKIKNWKEVGGQDLDISIINRAASSGS
                     RATFDNTIMGNVAPIQSQEQDSNGMVKSIVSQTPGAISYLAFAYVDKSVGTLKLNGFA
                     PTAKNVTTDNWKLWSYEHMYTKGNETGLTKEFLDYMKSDKVQSSIVQHMGYISINDMK
                     VVKDAEGKVTPK"
     gene            9197..10114
                     /gene="pstC"
                     /locus_tag="VBD27_000404"
     CDS             9197..10114
                     /gene="pstC"
                     /locus_tag="VBD27_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO7024440.1"
                     /translation="MKNQELAKKLTSPSKNSRLEKFGKGITFLSLALIVFIVAMILIF
                     VAQKGLSTFFVDGVKLTDFLFNTKWEPSAKSFGALPMIAGSFIVTILSAIIATPFAIG
                     AAVFMTEISPKYGSKILQPAVELLVGIPSVVYGFIGLQIIVPFVRSIFGGTGFGILSG
                     VCVLFVMILPTVTFMTVDSLRAVPRHYKEASLAMGATRWQTIWRVILNAARPGIFTAI
                     VFGMARAFGEALAIQMVVGNSAILPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLIMSLAFNTVIKLITREGKKNYER"
     gene            10104..10991
                     /gene="pstA"
                     /locus_tag="VBD27_000405"
     CDS             10104..10991
                     /gene="pstA"
                     /locus_tag="VBD27_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO7024441.1"
                     /translation="MNAKKADKLATTILYSIAAIIVTILASLLIFILVRGLPHVSWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSLGAGIYLSEYAKKGRLTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFQYGFSIISGALALTVFNLPQMTRSVEDSLQNVH
                     HTQREAGLALGISRWETVIYVVVPEALPSIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNVLSVTSPISIFRQAETLAVHIWKVNSEGTIPDATQVSAGSAAVLLVVILIF
                     NLSARSIGKKLHSKLTSSN"
     gene            11003..11806
                     /gene="pstB"
                     /locus_tag="VBD27_000406"
     CDS             11003..11806
                     /gene="pstB"
                     /locus_tag="VBD27_000406"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7024442.1"
                     /translation="MAEYNWDERHIITFPEENSALTTKDLHVYYGEKEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGQIMYEGIDVNAQDINVYEMRKH
                     IGMVFQRPNPFAKSIYKNITFAYERAGVKDKKFLDEVVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAIAVKPEILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTNNIFQNAKCQSTSDYVSGRFG"
     gene            11818..12576
                     /gene="pstB"
                     /locus_tag="VBD27_000407"
     CDS             11818..12576
                     /gene="pstB"
                     /locus_tag="VBD27_000407"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7024443.1"
                     /translation="MTQPILQVSDLSVYYNKKKALKEVSMDFYPNEITALIGPSGSGK
                     STLLRAINRMGDLNPEVTLTGAVMYNGHNVYSPRTDTVELRKEIGMVFQQPNPFPMSV
                     FENVVYGLRLKGIKDKATLDEAVETSLKGASIWDEVKDRLHDSALGLSGGQQQRVCIA
                     RTLATKPKIILLDEPTSALDPISAGKIEETLHGLKDQYTMLLVTRSMQQASRISDRTG
                     FFLDGNLIEYGNTKEMFMNPKHKETEDYITGKFG"
     gene            12610..13263
                     /gene="phoU"
                     /locus_tag="VBD27_000408"
     CDS             12610..13263
                     /gene="phoU"
                     /locus_tag="VBD27_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO7024444.1"
                     /translation="MLRSKFDEELDKLHNQFYAMGIEAIGQIKKTVRAFVSHDRELAK
                     EVIEDDVTLNNFETKLEKKSLEIIALQQPVSQDLRTVITVLKATSDVERMGDHAAAVA
                     KATIRMKGEERIPAVELEINNMGKAVKNMLEEALTAYINGDDEKAYEVAAMDEIVDDY
                     FRDIQKMVVETIQKHPDVAFAAKEYFQVLMHLERIGDYGKNICEWIVYLKTGKIIEL"
     gene            13409..15958
                     /locus_tag="VBD27_000409"
     CDS             13409..15958
                     /locus_tag="VBD27_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEO7024445.1"
                     /translation="MKTVEHFVTKFVPENYNLFLDINRQTKTFSGNVAVSGEALDNNI
                     SFHQKGLTIKSVLLDNQPLDFQLDEDNEAMHIQLHETGSMVLVFEFSGHITDNMTGMY
                     PSYYTVNGIKKEVISTQFESHFAREVFPSIDEPEAKATFDLSLKFDQKEGEIALSNMP
                     EINAEQRQETGLWTFDTTPKMSSYLLAFALGELHGKTTHTKNGTLVGSYATKAHQLNE
                     LDFSLDIVVRVIEFYEDYFGVRYPIPQSLHVALPDFSAGAMENWGLVTYREVYLLVDE
                     NSSVSSRQQVALVVAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIDYIEPKLN
                     IFEDFQTGGLPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRW
                     LGDTDFAAGLKIYFEKHQYQNTIGRDLWNALSQTSGKDVAAFMDSWLEQPGYPVMAAK
                     IEEDELILTQKQFFIGEHEDKSRLWQIPLNSNWEGIPEILTEETVVIPNFSQLAEKNK
                     ENGALRFNTENTAHYITNYQGQLLEHIISDLPLMDNISKLQIVQERHLLAESGMISYS
                     SLIPLVSLLSQETSYLVNSAIKSVIDGLSLFVQEDSQDEFDFKEFVNKLSAFNFNRLG
                     FEKREGEGDDSEMVRHLSLSLALYSDNEHAIEEAHHIFKAHENNIAAIPAAIRLLVLT
                     NEMKHFESKELSHLLLETYSTTTDGNFKRQLASALSHTTDSKTLKKLLSDWKNKDIVK
                     PQDLAMSWYATFLKNSFTQESVWEWAQENWEWIKATLGGDMSFDKFVIYPSSSFKTEE
                     RLEQYKNFFEPQLSDMAISRNISMGIKEISARVLLITKQKEEVINTIKKYI"
     gene            16120..16800
                     /locus_tag="VBD27_000410"
     CDS             16120..16800
                     /locus_tag="VBD27_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7024446.1"
                     /translation="MIKILLIEDDLSLSNSVFDFLDDFADVMQIFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESIDDKGQGFDLGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNDNSLIYGDIRVDMSTNSTFVNQTEVELLGKEFDLLVY
                     FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKVRKKLKGTLFSENLQTLRSVGY
                     ILKHVETD"
     gene            16784..18097
                     /locus_tag="VBD27_000411"
     CDS             16784..18097
                     /locus_tag="VBD27_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7024447.1"
                     /translation="MSKPIKISKFKKNVSDSHFIHFFTVFSGIFLVMTVIILQVMRYG
                     VYSSVDSSLKYISTHPKNYINMVMSRTAAYLDNSNIASVKLKPGGQTVANTDIILFTS
                     EEEVINYFDAFSNYQFLKPNKKNLGGISELTLTNIFGQDETYHAVTVKVNNPAYPNVT
                     YMTAIVNIDQLVNAKERYEKIIIFVMTTFWIISIGASIYLAKWAQKPIIENYERQKAF
                     VENASHELRTPLAVLQNRLETLFRKPNATILENSENIASSLDEVRNMRILTTNLLNLA
                     RRDDGIKPELAVIKPTLFDSIFENYDLIAQENGKNFTGHNMIQDSFKTDKTLLKQLMT
                     ILFDNAIKYTDNDGSIDFTISETDKYLFLEIADNGPGISEEDKVRIFDRFYRVDKART
                     RQQGGFGLGLSLAQQIVNSLRGNITVIDNKPRGSIFKIKLPKVKG"
     gene            18186..18518
                     /locus_tag="VBD27_000412"
     CDS             18186..18518
                     /locus_tag="VBD27_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEO7024448.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     SGVSRQAVYDNIKRTEKILEAYEMKLHMYSDYIVRSQIFDDILEKYTDDAFLQEKISI
                     LSSIDNRD"
     gene            18536..20101
                     /gene="ffh"
                     /locus_tag="VBD27_000413"
     CDS             18536..20101
                     /gene="ffh"
                     /locus_tag="VBD27_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO7024449.1"
                     /translation="MAFESLTERLQGVFKNIRGKKKLSEKDVQEVTKEIRLALLEADV
                     ALPVVKTFIKHVRERAVGHEIIDTLDPTQQIVKIVNEELTDLLGAETSEIEKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEDNARPMMIAADIYRPAAIDQLKTLGSQINVPV
                     FDMGTNHSAVEIVTKGLEQARENRNDYVLIDTAGRLQIDATLMQELHDVKAIAQPNEI
                     LLVVDSMIGQEAANVAEEFNRQLSISGVVLTKIDGDTRGGAALSVREITGKPIKFTGT
                     GEKITDIETFHPDRMASRILGMGDLLTLIERASQEYDEKRSMELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMLPGMANNPAMKNFKVDENEIARKRAIVSSMTPEERENPN
                     LLNPSRRRRIAAGSGNTFVDVNKFIKDFNQAKQMMQGVMSGDMNKMMKKMGIDPNNLP
                     KDMPGMDGMDMSNLEGMMGQNGMPDLSSLGGDMDFSQMFGGGLKGKVGAFAAKQSMKR
                     MANKMKKAKKKRK"
     gene            20168..20854
                     /locus_tag="VBD27_000414"
     CDS             20168..20854
                     /locus_tag="VBD27_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEO7024450.1"
                     /translation="MYLALIGDIINSKQILERETFQQSFQQLMTELSDVYGEELISPF
                     TITAGDEFQALLKQSKKVFQIIDHIQLALKPVNVRFGLGTGNIITSINSNESIGADGP
                     AYWHARSAINHIHDKNDYGTVQVAICLDDEDQNLELTLNSLISAGDFIKSKWTTNHFQ
                     MLEHLILQDNYQEQFQHQKLAQLENIEPSALTKRLKASGLKIYLRTRTQAADLLVKSC
                     TQTKGGSYDF"
     gene            20844..21617
                     /locus_tag="VBD27_000415"
     CDS             20844..21617
                     /locus_tag="VBD27_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO7024451.1"
                     /translation="MISDFLRDNPILTLLFCAHFLADFQWQSQSLADSKSHSWRGLWR
                     HLLIVFLPLAALMILIPETTLLNLSIWGSHIVIDSIKKLSYPWVEEGHFQKAAFIIDQ
                     LAHYTCIIVFYHALPTYLPPNHWLLPIKHFIVIALVFIIITKPINIVFKIFFNKFQAK
                     ELSSLLTQEKTKIMKEKSEDHEETIEGAGAMIGNLERLIMAILLISGQYAAIGLVFTA
                     KSIARYDKISKSQVFAEYYLIGSLFSIISVLITHWLLLV"
     gene            complement(21650..23245)
                     /locus_tag="VBD27_000416"
     CDS             complement(21650..23245)
                     /locus_tag="VBD27_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7024452.1"
                     /translation="MSSFNRKKLKFLGISLATLTATTVTLVACGNESKNSGDNKVINW
                     YIPTEISTLDISKNTDAYSNLAIGNSGSNLLRIDKEGKPKPDLAKKVSVSSDGLTYTA
                     TLRDNLKWSDGSKLSAEDFVYTWRRIVDPKTASEYAYLATESHLLNADKINSGDIKDL
                     NKLGVTAKGNQVTFKLTSPCPQFKYYLAFSNFMPQKQSYVEKVGKDYGTTSKNQIYSG
                     PYLVKDWNGSNGKFKLVKNKYYWDSKHVKTNSVIVQTIKKPDTAVQMYKQGQIDFAEI
                     SGTSAIYNQTGSVKALTNQKIRQALNLATDRKGVVKAAVDTGSTPAESLVPKKLAKLP
                     NGEDLSKYTAPGYTYNTSKAQKLFKEGLAEVGQSSLKLTITADSDSPAAKNAVDYVKS
                     TWESALPGLTVEEKFVTFKQRLEDAKNENFDVVLFSWGGDYPEGSTFYGLFTTNSAYN
                     YGKFSSKEYDNAYQKAITTDALKPGDAANDYKTAEKALFDQSYYNPVYYLGKKGLQNS
                     KLKGLVRNSTGLNVDFTYAYKTE"
BASE COUNT         7867 a         3931 c         4278 g         7194 t
ORIGIN      
        1 gagaaagtct atttttgagg aaactgcaaa aggtaaagta tcaaggcatg tccttttaac
       61 tatattattg gctttgatat tactatttac agcagatttc cttacctata ttattattga
      121 attgattatc tcgcttgatt tttggaaact agagtgggat agcgatatcg tccagttaat
      181 cattttacta gcaacttcaa ttagtacggt aatgttattt atgtgggtga aatttaaaga
      241 gaagcgtcca attagttctc tagggctatt taaagaagga gccctaacag aattatctag
      301 aggatggtta gtcgggagcg ttctctttgg agcctgctta atattaacaa taatttcagg
      361 ctctggagtt atcgaaactg ttcgttttac agctgataaa ttgatttggt ttcttatttt
      421 tgctgttgga tggcaggtac aaagcgcatc tgttgaaata ttgtgtcgtg gatggttact
      481 tccggtgagt gccacaaagt atagtaagat agtttctgtc agtatcagtt caatcttttt
      541 cggtcttctg catagcgcta ataatcatgt aagccttatc tcgattttca atctgtgtct
      601 ctttggacta tttctatcgc tctatgttat tttaaaaggt aacatctggg gggcgtgtgg
      661 tattcatggc gcctggaact gtgtacaagg gagtgtcttt ggaatcgaag ttagtggaga
      721 gccaatgttg tctaactccc tagtccatgt taaaacctat ggtgctgact ggattagcgg
      781 tggaaaattt ggtgtggaag gaagcatgat taccagtatt gttcttatcg tagcatgtta
      841 ttggctttat caaaaatcga attttcctta cgactaatag atttaatcta aataagcttc
      901 tcttactgaa agcttatttt ttattttagt attaaaaaac tatgttttgt gatataatat
      961 tttaaagacg aaaggagtct attgtgccac aaaatccaac aagtaaccca gagaaaaaaa
     1021 cttatttatt atctgaattg gtagactgct ttaaaggtaa agctgtgcct tcaaaagcag
     1081 aagcaggtga tatccgtatt attaatttga gtgatatgtc tcctttagga attgactacc
     1141 ataacttaag aacattccaa gatgaacaac gatcgctttt aaaatatcta cttcaggaag
     1201 gcgatgtttt aatcgcttca aaaggaactg tcaagaaagt tgctattttt gaagagcaag
     1261 attaccctgt cgttgcctct gctaatatta caatactaag gcctacacaa cacattagag
     1321 ggtactactt aaagcttttt tttgattcag aagaaggtca acaggcactt gagaatgcta
     1381 acaaaggaaa agctgtgatg aatattagca caaaggaact gttaaatatt gcaataccaa
     1441 gtatcccgct ttttagacag gattatctta tccaacgtta taaacagggg ctcaacgatt
     1501 acaaacgtaa gatagcacgt gcagagcagg agtgggaacg tatccaaaat gacatcagac
     1561 aacaacttag ctagaaccaa atacaaaaca tcataggaag agttatcatg aacgaaatca
     1621 aatgccctca ttgtggaaca gcttttgcca tcaacgagtc tgaataccat caattactag
     1681 aacaaattcg tggagatgct tttgacaaag aagtaagtga acggttggaa aaagaacgtc
     1741 taatattagg ggagcaagca aaaaatcaat tacaggaagt tgttgtagaa aaagacaagg
     1801 agatagctaa acttcagtac aaagtcaaac aatttcttat agaaaaagac aatcttctca
     1861 aagacaatga gtaccaactc gctgagcaat taaatcaaaa agacatgatg cttcgcgacc
     1921 ttgaaaacca aatcgataga ctacgtttag agcatgaaaa tagcttgcaa gaggcgctaa
     1981 caaaagtcga acgagaaaga gatgcaatac aaaatcagtt gcacattcaa gaaaaagaaa
     2041 aagatttagc tttagcttca gtaaaaagtg attatgaagt acaactaaag gcagccaatg
     2101 aacaagtaga attctataaa aacttcaaag ctcaacagtc tactaaagca gtaggagaaa
     2161 gtttagaaca ttatgctgaa acagaattta ataaagtgcg acatttggcc tttcctaatg
     2221 cttattttga gaaggacaat acattatcaa gtcgtggctc aaagggggac tttatctatc
     2281 gagaaaagga tgaaaatgac cttgagtttt taagtatcat gtttgaaatg aaaaatgagt
     2341 ctgatgatac tatcaagaag cataaaaatg aagatttttt caaagaatta gataaagatc
     2401 gtcgtgaaaa atcttgcgaa tacgcagttt tagtaactat gcttgaagca gacaatgact
     2461 attataatac tggaattgtt gatgttagtc acaaataccc taaaatgtac gttatacgtc
     2521 cacaattttt tatccaatta attggtattc taagaaatgc agcactcaat accttaaaat
     2581 ataaacaaga gcttgctttg atgaaagaac aaaatattga catcacacat tttgaagaag
     2641 atttagatat tttcaaaaat gcatttgcta aaaattataa ttctgcaagc aaaaatttcc
     2701 agaaagcaat cgatgaaata gataaatcta ttaaacgtat ggaagctgtt aaggctgctt
     2761 taacaacgtc tgaaaatcaa ctacgtcttg caaataataa attagacgat gtttctgtca
     2821 agaaattaac aagaaaaaat ccaacaatga aagcaaaatt cgatgctcta aaagactaag
     2881 aggttatgat tatgaagatt agaactgcta cactagacga tagtgaaaaa ttagtccctt
     2941 tgtatcaaga attaggatac gcaatatcat tatcagagat tcaatctatt ttaaaggtta
     3001 ttcttactca ttcagattat ggctttttga tagctgagga taatggcaaa ctactcgcct
     3061 tcgttggata tcataaactt tacttttttg aaaaaagtgg gacttactat cgtattttag
     3121 cactagtagt taatgaaaag caccgtcgta aaggcatcgc tagtcaactt attaatcacg
     3181 ttaaacagct agctaaaaca gatggaagtg aagtactggc tttaaatagc agtttaaaag
     3241 aatatcgtca ggaagcttat catttttatg aaaacctagg gttcaagaag gttagtacag
     3301 gcttttcata ctatttagat taggtataaa tccttatagc actataccaa aagagcgtaa
     3361 gtttttacgc tcctttcttt gtgttaccgc ttatttactg atataatata aaacatgatt
     3421 acaggaataa ttaatttaaa gaaagaagct ggaatgacat cacacgatgc tgtattcaag
     3481 cttcgaaaga tattacatac aaaaaaaata ggacacggcg gaactcttga tccagatgtt
     3541 gttggggttt taccaattgc ggttggaaaa gccactcgtg ttattgaata tatgacagaa
     3601 agtggcaaaa tttatgaagg agaaatcact ttaggatatg ccacaagtac agaagattct
     3661 agtggagagg tcatttcaag aactccacta actcaatccg atcttagcga ggatgttgtg
     3721 gatcatgcga tgaaaagttt taccggtcca attacacaag ttcctcctat gtattcagca
     3781 gttaaagtaa atggtaaaaa actctatgaa tatgctcgtt caggagaaga agtagagcga
     3841 cctaaacgac aaataacaat ttctgaattt agacgcacaa gcccattgta cttcgaaaaa
     3901 ggcatctgcc gttttagttt ctatgtctca tgcagtaaag gtacctatgt taggacgtta
     3961 gctgttgatc taggaataaa actaggatat gctagccata tgtctttcct aaaaagaacc
     4021 tcatctgcag gattatctat tacacaatca ttaacgcttg aagaaattaa tgaaaaatat
     4081 aagcaagaag atttttcatt tttacttcca attgaatatg gtgttttaga tctcccaaaa
     4141 gttaacctaa ctgaggaaga taaagttgaa atctcatacg gacgtcgtat cttattagaa
     4201 aatgaagcag ataccttagc agctttttat gaaaatagag ttatcgctat actagaaaaa
     4261 cgtggcaatg aatttaaacc acacaaagta ttgttataaa gaaagtcaat catgaaaatc
     4321 cattacatta atgattataa agatattcaa gctaaagaag attgcgtctt agttttgggc
     4381 tattttgatg gcttacatct gggacataaa gccctctttg ataaagctaa aaaaatcgcg
     4441 accgaaaaaa atttgaaaat tgtcgtctta acgtttaatg agacacctcg tctaaccttc
     4501 gctcgcttcc aacctgaatt attgctacat cttacctctc cagaaaaacg ttctgaaaaa
     4561 tttcaagagt atggcgtcga tgagttatat ttaatgaatt ttaccagtca tttttcaaaa
     4621 gtatcttctg acctttttat taagaaatat atttatggtc tcagagcaaa agctgctgtt
     4681 gtcggttttg attataagtt cggtcataat cgtaccagtg gtgattatct agcgcgtaat
     4741 ttcaagggac cagtctatat tattgatgaa atttctgaag gtggtgaaaa aatctcttct
     4801 acacgtattc gtcaattgat tacagagggt aatgttgaga aagctaacca acttttgggt
     4861 tatgagtttt caacctgtgg catggtagta catggagatg ctagaggacg aactataggg
     4921 ttcccaactg ctaatctagc tcctattaat cgcacttact tacctgcaga cggtgtttat
     4981 atttccaatg ttctgattaa tggaaaatat tatagagcaa tgacgagcat tggaaaaaat
     5041 atcacttttg gtggtacaga acttcgttta gaagctaata tctttgattt tgatggcgac
     5101 atttatggag aaactattga aattttctgg ttaaaaagaa tcagagaaat ggtaaagttt
     5161 aatggtattg acgatcttgt aaaacaactt aaaaaagata aagaaattgc tttaaattgg
     5221 aaaaaagata gtcaaactct ttaatttttt gtataatagt ctctagtaaa tacaagaggc
     5281 ggttatatga ttaccttatt tttatcaccc agctgtacta gttgtcgaaa agcgcgtgct
     5341 tggctaagta agcatgaggt tgcttttgaa gagcataaca ttattacaag tccactcaat
     5401 aaagaagaac tattacaaat tctatcgttt acagaaaatg gtacagaaga cattatttcc
     5461 acacgttcaa aagtctttca aaaacttgct attgatgtag acgaattatc aacgtcttca
     5521 ttaatggaat taatttcaga aaatccaagt cttttacgac gcccaattat tttagacaaa
     5581 aaacgtatgc agattggttt taatgaggat gaaattcgtg ccttcttacc gcgtgactat
     5641 cgtaaacaag aactgaaaca ggccactatt cgtgctgaaa ttgagggaaa acatgattag
     5701 tagtaattat agttatcctc ttgatccttc atggaatacg gaagatatta ctaaggtact
     5761 tcgcttttta aatcaagttg aacacgccta tgaaaatagt gttaaagttg atgatttgct
     5821 agatagctat aaagaattta aaaaatttgt taaatccaag gcgcaggaaa aacaaattga
     5881 ccgtgaattc cagagaacta gcggttattc cacttatcaa gctgttaaag cagcacaaca
     5941 acaagcgaaa ggatttattt cacttggacg ctaaatttga tttcgctaaa caattagtgt
     6001 ataaagctgg tcaattcatt aaatctgaga tgcaaaatac ttttgatgtt gaggaaaagt
     6061 ctcgatttga tgatttagtg acaagtcttg ataaaaaaac acaaaaatta ttaattcaag
     6121 aaattattca acattaccca gatgataata tacttgccga agaagatgat gtgcgctcac
     6181 ccattgcaca aggtaatgtt tgggttttag accctattga tggaactgtt aattttattg
     6241 ttcaaaaaga taactttgct gttatgttag cttattacga agaaggcgtt ggacaatttg
     6301 gtatcatcta cgacgttatg gctgatattc tttacagcgg gggaggacat tttgatgtct
     6361 atgctaacga taaaaaaatt gttccttttc aagagtgtcc tttagaacgt tgcttacttg
     6421 gagtaaattc tgctatgtat gcagaaaatg attgcggtat tgcccattta gcttctgaaa
     6481 ccttaggtgt tcgtatctat ggtggtgctg gaattagcat ggcaaaagtc atgcagggca
     6541 aattattagc ttatttttcg tatattcaac cttgggatta tgctgcagca aaaatcatgg
     6601 gggagacatt aggatttacc ttattaacgc ttgacgggga agaacccaac tattcaacaa
     6661 gacaaaaagt aatgttccta ccaaagtcga aattaaatct tatccaatcc taccttacca
     6721 aaaaataaac ttaaaacctt ctaattaaat agaaggtttt tttgcacgcc cgtgattatg
     6781 tagtataata gaaacatgaa attaccgaat gaatttattg aaaaatatca gactatactt
     6841 aaagatgaag cagaagcttt ttttgacagt tttgaacaaa aacccatatc ggcatatcgc
     6901 accaatcctc tcaaagaaaa gcaacttgat tttccaaatg ccatcccaag taccccttgg
     6961 gggcattatg ggaaaatatc aggaaaatca atagaacaca caactggcct tgtttattct
     7021 caagaacctg ctgctcagat agtggctcag attgctgaac cacaagaagg aatgaaagtg
     7081 ctagatttag cagctgcacc tggtggaaaa acaacacacc ttctatcata tttaaacaat
     7141 actggtctat tagttagtaa tgaaatttca aataaacgta gtaaaatttt agtcgaaaat
     7201 gtcgaacgtt ttggtgctag aaatgttatc gtgactaatg aaagctccca acgactagcc
     7261 aaatgtttta attctttttt tgatcttatt gtttttgacg gtccgtgttc tggtgaggga
     7321 atgttccgca aagatcctca agcaatacaa tattggcaca aagattaccc cactgaatgt
     7381 gcacagttac aaagagatat tttaaaagaa gcaatcaaaa tgctagctca tggtggtata
     7441 cttgtatact ctacttgtac atggtcacca gaagagaacg aagaagtggt taattggctc
     7501 cttcaagagt atgattactt agaacttgtt gatattccta aactaaatgg gatggttgaa
     7561 gggattaatg taccacaagt tgcaaggatg taccctcatc attttcaagg tgaaggacaa
     7621 ttcgttgcaa aactgagaga tactagatct aaggaagcac agaaaattaa gccaaaagca
     7681 cagaaaataa ataaaatgca gttacaattg tggcaacaat ttgcacaaga ccatttaaag
     7741 atagacttga acggtgtttt agatgttttc ggcgaccaac tctatcttct acctaatggt
     7801 ctccctgatt tgtctaaatt aaaaatagca cgcaatggtc tccatctagg cactttcaag
     7861 aagaatcgtt ttgagccatc gtttgcttta ggaatggcac ttagcgaaca tgacctagta
     7921 cagtctattg aaattgatat agaacagttt gaggtgtacg tatctggaaa tgtagtcaaa
     7981 ctagacaaga ctgttccaaa cggttggtat caaattcttg taaaaggcaa tggattaggt
     8041 tttgcaaaag tgacaaataa tactcttaaa aattattacc ctaaaggact acgatttcag
     8101 acataactgt tattgaatac ttttaataaa aaaggaaaaa gaaatacttt tcaaacccta
     8161 gaaacttatg gtactatggg acagtgatgt gtgtttttac tatttgtaaa tcattaaaca
     8221 ctcaatgatt ttagtaatat atatcaacta ctaaatatca aaatttattc aaaagaatag
     8281 gaaaatgaaa atgaaaaaaa acattttttt gttaaccttg ctagccaccg tcttattgac
     8341 gctgtctggt tgtgctaatt ggattgataa aggtcaatca atcaccagcg ttggttcaac
     8401 cgccttacag ccacttgtag aagctgcagc agatgagttt ggtaagacaa acctcggtaa
     8461 gactatcaat gtccaaggag gaggctcagg aacaggctta tcacaagtac agtctggagc
     8521 tgttcaaatt ggtaatagcg acttatttgc agaagaaaaa gatggtattg atgctaagaa
     8581 attgaaagac catcaagtcg cagtagctgg attagctgtt attgttaata aaaaagttaa
     8641 tgttaaaaat ctaaccaccc accaacttcg tgatattttt gcaggaaaaa tcaagaattg
     8701 gaaagaagtg ggaggacaag atttagatat ttccatcatc aatcgagcag caagttctgg
     8761 atcccgtgca accttcgata atactattat gggaaatgtt gctcctattc aaagccaaga
     8821 acaagattca aatggtatgg tgaaatcaat tgtttctcaa acacctggtg cgatttcata
     8881 cctcgctttt gcatacgttg ataaatctgt cggaacatta aaactaaatg gttttgcgcc
     8941 aacagctaaa aatgttacta ccgacaactg gaaactttgg tcatatgaac atatgtatac
     9001 aaaaggtaat gaaacaggtc taacaaaaga attcttagac tacatgaaaa gtgataaagt
     9061 tcaaagtagt atcgtacaac atatgggata tatctctatt aacgacatga aagttgttaa
     9121 agatgctgaa ggtaaagtaa ccccaaaata aaacttaatc acatctagat aaagcatctt
     9181 gatcctagga ggaattatga aaaatcaaga attagctaaa aaattaacat ctccttcaaa
     9241 aaactctcga ttagaaaaat ttggtaaagg aataactttt ctttcgcttg ccctaatcgt
     9301 cttcattgtt gcgatgattt tgatttttgt tgctcaaaaa ggactatcta cattttttgt
     9361 tgatggcgtt aaattaacag acttcttatt taatacaaaa tgggaaccaa gtgctaagtc
     9421 attcggtgct cttccaatga tagctggatc atttattgtt actattctat cagctatcat
     9481 tgctacacca tttgctatcg gtgctgccgt atttatgact gaaatctcac ctaaatatgg
     9541 ttctaaaatt ttacaacctg cagttgaatt actagttggg attccttctg ttgtttacgg
     9601 ttttattggt ttacagatta ttgttccctt cgttcgctct atttttgggg gaacaggttt
     9661 tggcatttta tctggggtct gtgtcctctt cgtcatgatt ctaccaactg ttactttcat
     9721 gacagtagat agccttagag ctgtgccacg tcactataaa gaagcaagtt tagccatggg
     9781 ggcaactcgt tggcaaacaa tttggcgtgt tatattaaac gctgcacgtc caggaatttt
     9841 cactgcaatt gtttttggta tggcacgtgc atttggtgaa gctctagcaa ttcagatggt
     9901 tgtcggaaac tctgcaattt taccaacatc attgacaaca ccagcagcaa cattaacgtc
     9961 tgttctaaca atgggtattg gtaatactgt aatgggaact gtacaaaaca atgtcctctg
    10021 gtcgctagcc ttagtacttt taattatgag tttagcattt aatacagtta ttaagttaat
    10081 tacaagggaa ggaaagaaaa actatgaacg ctaaaaaggc tgacaaatta gcaactacaa
    10141 tactctatag tattgcagct atcatcgtta caatcctcgc ctctctttta atttttattc
    10201 ttgttcgcgg attgccacac gtatcttggt catttttaac tgggaaatca tcatcatatg
    10261 aagcaggagg cggtattgga atacaattat acaattcctt tttcctactt attgttactc
    10321 tgattatttc tattcctcta tctttaggag ctggaatata cctctcagaa tatgctaaaa
    10381 aaggacgttt aacaaacttt gttagaacat gtatcgaaat tttgtcatct cttccttctg
    10441 ttgtagttgg tctgtttggt tacctaatct tcgttgttca atttcaatat ggcttctcaa
    10501 ttatctcagg tgccttagca cttactgttt ttaacttacc ccaaatgaca cgtagtgttg
    10561 aagatagcct tcaaaatgta caccacacac agcgtgaagc aggattggcc ctaggtattt
    10621 ctcgttggga aacggtcatt tacgtcgttg tccctgaagc tctgccaagt attgtaactg
    10681 gtgttgtgct agcttcagga cgtattttcg gtgaggcagc agcacttatt tatacagctg
    10741 gtcaatctgc tccagcactt gattggtcaa attggaacgt ccttagtgtt acaagtccaa
    10801 tctcaatttt ccgtcaagca gaaactttag ctgttcatat ttggaaagtt aatagcgaag
    10861 ggacaatccc tgatgcaaca caagtatctg ccggtagcgc cgcagtctta ttggttgtta
    10921 ttcttatttt caacctttca gctcgctcga tcggtaagaa acttcattcg aaattaacct
    10981 cgtctaatta aaaggagtag taatggcaga atataattgg gacgaacgcc atatcattac
    11041 cttccctgaa gaaaattcag ctctcaccac aaaagattta cacgtttact acggtgagaa
    11101 agaagctatc aagggaattg atatgcaatt tgaaaaaaat aaaattacag ccttaatcgg
    11161 cccatctgga tgtggaaaat caacatatct tcgtagctta aatcgtatga acgatacgat
    11221 tgatattgca cgcgtgactg gacaaatcat gtatgaagga attgatgtta acgctcaaga
    11281 tatcaatgtt tacgagatgc gaaaacatat cggaatggtt ttccaacgtc caaacccgtt
    11341 tgcaaaatca atttataaaa acattacctt tgcatatgag cgtgctggtg taaaagataa
    11401 aaaattctta gatgaagttg tcgaaacctc actaaaacaa gctgctctat gggatcaagt
    11461 aaaagatgac cttcataaat cagcattcac tttatctggc ggtcaacagc agcgattatg
    11521 tattgcacgt gctatcgcag ttaaaccaga aattctctta atggatgaac ctgcttcagc
    11581 actcgatccg attgcaacta tgcaactaga agagacaatg tttgaactca agaaaaatta
    11641 cactataatt atcgtaacac ataatatgca acaagcagca cgtgctagtg attatactgc
    11701 atttttctat cttggagact taatcgaata cgataaaacg aataacattt tccaaaatgc
    11761 taaatgtcaa tctactagcg attatgtttc tggtcgattt ggatagtgag gtaaaagatg
    11821 acacaaccta ttttacaagt cagtgatttg tctgtttact ataataagaa aaaagcttta
    11881 aaagaagtct caatggactt ctatcctaat gaaattacag ccttaattgg tccgtctggt
    11941 tctggaaagt caacactctt acgagctatt aaccgtatgg gagacttaaa tcctgaagtc
    12001 actctaacag gagcagtgat gtataacgga cataatgttt atagcccgcg cactgatacc
    12061 gtagaacttc gtaaagaaat tgggatggtt tttcagcagc ctaatccatt tcctatgtca
    12121 gtatttgaaa atgttgttta tggtcttaga ctcaaaggaa taaaagacaa agctacgtta
    12181 gatgaagccg tagagacttc attgaagggc gcgtctatct gggacgaggt caaagataga
    12241 ttacacgact ctgctcttgg tttatctggt ggtcaacaac aacgtgtttg tattgccaga
    12301 acccttgcta ccaagcctaa aattatcctt ctagatgagc caacctctgc acttgaccca
    12361 atttctgctg gtaaaattga agagacttta catggtctaa aagatcaata taccatgtta
    12421 cttgttacac gttctatgca gcaagcctca cgtatttctg atcgtacagg tttcttctta
    12481 gacggtaact taattgaata tggcaataca aaagaaatgt ttatgaatcc taaacataaa
    12541 gaaactgaag attacattac aggtaaattc ggttaatacc atataataag atcagaaaga
    12601 gaatacaata tgttaagatc aaaatttgat gaagaattag ataaactgca taaccaattc
    12661 tatgcaatgg gaattgaagc tatcggacaa atcaaaaaaa cagttcgtgc ttttgtcagt
    12721 catgatcgag agttagctaa agaagtgata gaagatgatg taacgcttaa caactttgaa
    12781 acgaagttag agaaaaaatc attggaaatt attgcactcc agcagcctgt atcacaagac
    12841 ttacgcaccg ttattaccgt tttaaaagct actagtgatg ttgagcgtat gggagaccat
    12901 gcagcagcag ttgcaaaagc aaccatccgt atgaagggcg aggaaagaat cccagcagta
    12961 gaattagaaa tcaataatat gggaaaagca gttaaaaaca tgcttgaaga agccttaaca
    13021 gcttatatca atggtgatga tgaaaaagct tacgaagtag cagcaatgga tgaaatagtt
    13081 gatgattact tccgtgatat ccaaaaaatg gtagttgaaa caattcaaaa gcatcctgac
    13141 gtagcatttg ccgccaaaga atacttccaa gtattgatgc acttggagcg aattggagat
    13201 tatggcaaaa atatctgtga atggattgtt tatttaaaaa ctggaaaaat tatcgagtta
    13261 tgatataatt attgcttgta gagtggtaat tgtaagtata gttcgtgcga tagaaaagct
    13321 gtaactatct tgttacagct tttctatctt ttctagccca tacaggacta gtaatgtttc
    13381 aaatgtttct gaattagtta ggagttatat gaaaactgta gaacattttg ttaccaaatt
    13441 tgtccctgaa aactataacc ttttcttgga tattaatcgt caaactaaaa ccttttcagg
    13501 taacgttgct gtgtctggcg aagccttaga taacaatatc tcttttcacc aaaaagggtt
    13561 aaccattaaa tctgtcttat tagataatca accattagat ttccaactag atgaagacaa
    13621 tgaagctatg catattcaat tgcatgaaac tggtagcatg gtattagtct ttgaattcag
    13681 cggtcacatt accgataata tgacaggtat gtatccatct tactatacag ttaatggcat
    13741 caaaaaagag gttatctcca cacaatttga aagtcatttt gctcgtgaag tattcccaag
    13801 tattgacgaa ccagaagcaa aagcaacctt tgatttgagc ctaaaattcg atcaaaaaga
    13861 aggagagatt gctctttcaa atatgccgga aatcaatgct gaacaacgtc aagaaacagg
    13921 tttatggaca tttgacacca cacctaaaat gtcctcatat ttattagctt ttgccttggg
    13981 ggaattgcat ggtaaaacaa cacataccaa aaacggcact cttgttggct catatgccac
    14041 aaaagcccat cagttaaatg aattagattt ttctttagat atcgttgtgc gtgttattga
    14101 gttttatgag gactactttg gtgtacgtta tcctattcct cagtcactcc acgttgctct
    14161 acctgatttt tctgctggag ctatggaaaa ttggggatta gtaacatatc gtgaagtcta
    14221 tcttttagta gatgagaact ctagtgtttc cagccgtcag caagtagcat tagttgtagc
    14281 acatgaaata gctcaccagt ggtttggtaa cctcgttact atgaaatggt gggatgattt
    14341 atggttaaat gaatcatttg ctaatatgat ggaatatgta tcaattgatt atattgaacc
    14401 aaaacttaat atatttgaag actttcaaac aggtggtcta ccattagctc ttaagcgtga
    14461 tgccacagat ggcgtccaat ctgttcacgt tgaggtcaat catcctgatg aaattaacac
    14521 actttttgat ccagctatcg tttatgcaaa aggtagccgc ctcatgcaca tgttacgtcg
    14581 ctggttaggc gatacagatt ttgccgcagg actaaaaatt tattttgaaa aacatcaata
    14641 tcaaaatact attggtcgtg acctttggaa tgcactttct caaacctcag gaaaagatgt
    14701 tgcagctttc atggattctt ggttagagca accaggatac cctgtgatgg cagccaaaat
    14761 agaagaagat gagctcattt taactcaaaa acaattcttt attggtgaac acgaagataa
    14821 gtctcgcttg tggcaaattc ctcttaatag taattgggaa ggaatcccag agatattaac
    14881 cgaggaaacg gtagtaatcc ccaattttag tcagttagct gaaaagaaca aagaaaatgg
    14941 tgcactgcgt ttcaatactg aaaatactgc acattacatt actaactatc aaggtcaact
    15001 cttagagcat atcatttctg atcttcctct tatggataac atttctaaac tacaaattgt
    15061 acaagaacgt catttacttg ctgaaagtgg tatgatttca tatagtagtt taataccttt
    15121 agtctctctt ttgagccaag agacatcata tcttgttaac tctgctatta agagtgttat
    15181 tgatggttta tccttattcg tccaggaaga tagccaagat gaattcgact ttaaagaatt
    15241 tgttaataaa ttatcagcat ttaattttaa tagacttggc tttgaaaaaa gagaaggaga
    15301 aggagatgat agcgaaatgg tccgtcatct atccctaagc cttgctctct actcagataa
    15361 tgaacatgct attgaagaag ctcaccatat ttttaaagcc cacgaaaata atatcgctgc
    15421 tataccagct gcaattcgcc tgttagttct taccaatgaa atgaagcatt tcgaatccaa
    15481 agaacttagc cacttgttat tagagactta tagtactact accgatggga atttcaaacg
    15541 acaactagcg agtgctttat ctcatactac agatagcaag acactcaaga aattattgtc
    15601 agattggaaa aataaagata ttgtgaaacc acaagactta gctatgagtt ggtatgctac
    15661 tttccttaaa aattctttta cacaagaaag tgtctgggag tgggcccaag aaaactggga
    15721 atggatcaaa gcaactctag gtggtgatat gagttttgat aaatttgtga tttatccctc
    15781 aagtagtttc aaaacagaag aacgccttga acaatataaa aactttttcg agcctcaatt
    15841 aagcgatatg gcgattagcc gtaatatttc aatgggaatt aaagaaattt ctgctcgtgt
    15901 ccttttaatc acgaagcaaa aagaagaagt tatcaatact atcaaaaaat atatctaata
    15961 aactcaaata gagattgatg gattattcaa tctctatttc tttgtctgtg tcatgaaatt
    16021 agttgacata aaacatactt tatttaggat ttattaagca aagtttggta ttctaatatt
    16081 aatagttcag ataaaatatg tacatcgaaa ggatatacta tgataaaaat tttattaatt
    16141 gaagatgact tgagtctttc caattccgtt tttgatttcc ttgatgactt tgccgatgtc
    16201 atgcaaattt ttgatggaga agagggtctt tacgaagccg aaagtggagt ctatgatctg
    16261 attcttctag atctcatgtt acctgaaaaa aacggcttcc aagtgttgaa agagctacgt
    16321 gaaaaaggta ttacaacccc tgtattaata atgaccgcta aagaaagcat tgatgataag
    16381 ggacaaggtt tcgacttagg tgctgatgat tatctaacaa aaccttttta cctagaagaa
    16441 ctaaaaatgc gaattcaggc tcttttaaag cgttctggaa aatttaatga taacagccta
    16501 atttatggtg atattcgcgt agacatgtct actaactcaa cctttgttaa tcaaacagaa
    16561 gttgagctct taggtaaaga gtttgatctt ttagtctatt tccttcaaaa tcaaaatgtt
    16621 atcttaccga aatctcaaat ctttgatcgt atttggggat ttgatagtga tacaacgatt
    16681 tcagttgtag aagtctatgt ctctaaggtt cggaaaaaat taaaaggaac acttttttca
    16741 gaaaaccttc aaactttgcg tagtgtagga tatattttaa agcatgtcga aaccgattaa
    16801 gataagtaaa ttcaaaaaaa atgtctcaga tagtcatttt atccattttt tcacagtttt
    16861 tagtggtatt tttctggtta tgactgttat catcctccaa gtcatgcgct atggtgttta
    16921 ttcttctgtt gatagtagtt taaagtatat cagtacccat cctaaaaatt acatcaatat
    16981 ggttatgtca cgtactgctg cctacctaga taactccaat atcgcaagtg tcaagttaaa
    17041 acccggcggt cagacagtag ctaatactga tattattctt tttacatcag aagaagaagt
    17101 cattaactat tttgacgcat tttcaaacta tcagttttta aaacctaata aaaaaaatct
    17161 aggtggcatt agtgaattaa ctttaaccaa catctttggt caagatgaga catatcatgc
    17221 tgtcaccgta aaagttaata accctgctta tcctaatgtg acttacatga cagctattgt
    17281 taatattgat caattagtaa atgctaaaga acgctatgaa aaaattatta tatttgtcat
    17341 gactactttt tggattattt caattggagc aagtatctac ttagctaaat gggcacaaaa
    17401 accgattata gaaaactacg aaagacagaa ggcttttgtc gaaaatgcta gccacgagct
    17461 gcgtactccc ctagctgtct tacaaaatcg cctagaaacg ctattcagaa aaccgaatgc
    17521 caccatttta gaaaatagtg aaaatatcgc ttcaagtcta gatgaagtac gaaatatgcg
    17581 tattttaacc actaatcttt taaacttagc aagacgtgat gatggaatca aaccagaatt
    17641 agctgttatt aagcctacac tttttgatag tatctttgag aattatgatc taatcgccca
    17701 agagaatggt aaaaatttta ctggacataa tatgatccaa gatagtttca aaacagataa
    17761 gacactgcta aaacaactca tgactatttt atttgataat gctataaaat atactgacaa
    17821 tgatggtagc attgacttta ccatatctga aactgataag tatctctttt tagaaatcgc
    17881 tgataatgga ccaggaattt cagaggagga taaagtcaga atttttgatc gtttttaccg
    17941 tgttgacaaa gcaagaacac gacaacaagg agggtttggc ctcggccttt ctttagctca
    18001 acaaatcgtt aactcactgc gaggtaatat tacagtaatt gataataaac ctagaggcag
    18061 tatttttaag ataaaactac caaaagtaaa aggttaggcc atgttagtcc taacctttta
    18121 aattttaaag tggtcttgca tacttgatat agcaagctga taggaaattt ggtataataa
    18181 aagctatgga aattgaaaaa acaaatcgta tgaatgccct ttttgagttt tatgcggcat
    18241 tattaacaga taaacaaatg aattatatcg agttatacta cgccgatgac tacagccttg
    18301 ctgaaatagc tgaagaatcc ggtgttagcc gacaagctgt ctatgataat atcaaacgaa
    18361 ctgaaaaaat tcttgaggcg tacgaaatga aactacatat gtattcagat tatattgtcc
    18421 gtagtcagat ttttgatgac attctagaaa aatataccga tgatgccttt ttacaagaaa
    18481 aaatttccat tctaagtagc attgataata gagattaaat gaggagaaat aatttatggc
    18541 tttcgaaagt ttaacagagc gcctgcaagg agtttttaaa aatattcgtg gtaagaaaaa
    18601 gttatctgaa aaagatgtgc aagaagtcac caaggagatt cgtctagctc ttttagaagc
    18661 tgacgttgct cttccagttg ttaaaacctt tattaaacat gttcgtgaac gtgctgttgg
    18721 acatgaaatc attgatacac tagatcctac gcaacaaatt gttaagattg tcaatgaaga
    18781 gctaacagat ttattagggg ctgagacctc tgaaattgaa aaatccccta aaattccaac
    18841 catcattatg atggttggtc tacaaggtgc tggtaaaaca acttttgccg gtaagttagc
    18901 aaacaaacta atcaaagaag acaatgctcg tcctatgatg atagcagctg atatttatcg
    18961 accagctgct attgatcagt taaaaaccct tggtagccag attaatgttc ctgtctttga
    19021 tatgggaact aaccattcag cagttgagat tgtcactaaa ggtttagaac aggctcgtga
    19081 aaatcgtaat gactatgttc ttattgatac agccggacgc ctacaaattg atgcaaccct
    19141 tatgcaggaa ttgcatgatg tcaaagccat agctcaacct aatgaaatac tccttgtcgt
    19201 tgacagcatg attggacagg aagctgcaaa tgtcgctgaa gaatttaacc gtcagctcag
    19261 cattagtggt gttgttctga caaagattga cggtgataca cgcggtggtg ctgccctttc
    19321 agtacgcgaa attactggaa aaccaattaa atttacaggg acaggggaaa aaatcactga
    19381 tattgaaact ttccatcccg atcgtatggc aagccgtata ttggggatgg gagacctgct
    19441 aacacttatt gagcgcgcta gtcaagaata cgacgaaaaa cgttcaatgg aattagcgga
    19501 gaaaatgcgt gaaaacacct ttgacttcaa cgattttatt gatcaattag atcaagttca
    19561 aaatatgggg ccaatggaag atctccttaa aatgcttcca ggaatggcta acaatccagc
    19621 aatgaaaaac ttcaaagtgg atgaaaacga aattgctcgc aaaagagcca ttgtgtcatc
    19681 aatgactcca gaagagagag aaaatccaaa tctcctcaat ccaagtcgta gacgtcgtat
    19741 tgctgctgga tcaggtaata ctttcgttga tgttaataaa ttcattaaag attttaacca
    19801 ggcaaaacaa atgatgcaag gcgtgatgtc cggtgatatg aataaaatga tgaaaaaaat
    19861 gggaattgat cctaataacc tccctaaaga tatgcctgga atggacggaa tggatatgtc
    19921 taatttagaa ggtatgatgg ggcaaaatgg catgcctgac ttatctagtt taggtggcga
    19981 tatggacttc agccaaatgt ttggtggtgg cttaaaagga aaagtaggag cctttgcagc
    20041 taaacaatca atgaaacgta tggctaataa aatgaagaaa gctaagaaga aacgcaaata
    20101 aaaatgtcta tttaaataga catttttatt tttgtctgct ataatagaca aaaaggtggt
    20161 gatatttatg tatttagcat taatcggtga tatcattaat tcaaaacaga tacttgaacg
    20221 tgaaactttc caacagtctt ttcagcaact aatgaccgaa ctatctgatg tatatggtga
    20281 agagctgatt tctccattca ctattacagc tggtgatgaa tttcaagctt tattgaaaca
    20341 atcaaaaaag gtatttcaaa ttattgacca tattcaacta gctctaaaac ctgttaatgt
    20401 aaggttcggc ctcggtacag gaaacattat aacatccatc aattcaaatg aaagtatcgg
    20461 tgctgatggt cctgcctact ggcatgctcg ctcagctatt aatcatatac atgataaaaa
    20521 tgattatgga acagttcaag tagctatttg ccttgatgat gaagaccaaa accttgaatt
    20581 aacactaaat agtctcattt cagctggtga ttttatcaag tcaaaatgga ctacaaacca
    20641 ttttcaaatg cttgagcact taatacttca agataattat caagaacaat ttcaacatca
    20701 aaagttagcc caactggaaa atattgaacc tagtgcgctg actaaacgcc ttaaagcaag
    20761 cggtctgaag atttacttaa gaacgagaac acaggcagcc gatctattag ttaaaagttg
    20821 cactcaaact aaagggggaa gctatgattt ctgatttttt aagggataat ccaatcctaa
    20881 ctttattatt ttgcgcacat tttttagctg actttcagtg gcaaagtcaa tccttagcag
    20941 atagtaaatc acatagttgg aggggactct ggagacatct cttgattgtt ttcctccccc
    21001 tagcagcttt aatgatattg ataccagaaa ctactttgtt aaatttaagc atatggggaa
    21061 gtcatattgt tattgatagt ataaagaaat tgagttaccc ttgggtagag gaagggcatt
    21121 tccaaaaagc agcttttatt atagaccaac tagcccacta tacttgcatt atcgtttttt
    21181 accatgcgct tcctacatat ttaccgccta atcattggct tttgcctatc aagcacttta
    21241 ttgtcatagc tctggttttt atcattatca ccaaacccat taatattgtg tttaaaattt
    21301 tctttaataa atttcaagct aaagaattat catcattatt aacacaagaa aaaacaaaaa
    21361 taatgaaaga aaaatctgaa gatcatgaag aaacaattga aggagcagga gctatgattg
    21421 gtaatttaga gcgtctaatt atggctatat tactaatctc aggacagtat gctgctatag
    21481 gtcttgtttt tactgcaaaa tccattgcac gttacgataa aatttctaaa agtcaggttt
    21541 ttgccgaata ttatttaatt ggatcattgt tctcaattat aagcgttttg ataactcatt
    21601 ggttactttt ggtatagaaa agcgctagaa atatcctaac gcttttcttt tattctgtct
    21661 tataagcata agtaaaatca acattcaaac cagttgaatt tcttaccaac ccctttaatt
    21721 tagaattttg gagtcctttt ttccctaggt aataaactgg gttataatat gattgatcaa
    21781 ataaagcttt ttcagcagtt ttgtaatcat tagcggcatc tcctggtttc aaggcgtcag
    21841 tagtaattgc cttttgatag gcattgtcat actctttaga agaaaattta ccataattat
    21901 acgcagaatt agtcgtgaat aacccataga aagttgatcc ttctggataa tctcctcccc
    21961 atgagaagag cacgacatca aaattttcat tttttgcatc ttcaaggcgt tgcttgaaag
    22021 taacaaattt ttcttcaaca gttagaccag gcaaagcact ctcccatgta gatttcacat
    22081 agtcaacagc atttttagct gctggagagt ccgaatcagc tgtgattgtt aacttgagag
    22141 atgactgacc aacctcagca agaccttctt taaaaagttt ttgagcttta cttgtattat
    22201 acgtataacc tggggcagta tacttggaaa gatcctcacc attaggtaat ttggctaatt
    22261 ttttagggac cagagattca gcaggagttg aacctgtatc aactgccgct ttaacaacac
    22321 ctttacgatc agtcgctaaa ttcaaagctt gcctaatttt ctgatttgtc aatgctttaa
    22381 ccgaaccagt ttggttataa atagcagaag ttcctgatat ttcagcaaaa tcaatctgac
    22441 cttgcttgta catttgaact gcagtatctg gttttttgat agtttgaact atgacactat
    22501 ttgttttaac atgtttagaa tcccaataat atttattttt gactaattta aatttaccat
    22561 ttgaaccatt ccaatcctta acaaggtaag gacctgagta aatttgattt tttgatgttg
    22621 taccataatc tttaccaact ttttcaacat acgattgttt ttgaggcata aaattagaga
    22681 aggctaaata atatttaaat tgagggcatg gacttgtcaa tttaaaggtc acttgattgc
    22741 ctttagctgt tacaccaagt ttatttaaat ctttaatatc tccagaatta atcttatcag
    22801 catttaaaag gtgcgattct gtcgccaagt aagcatattc agatgcagtt ttaggatcaa
    22861 ctattctccg ccatgtgtaa acaaaatctt cagcgcttag tttgcttcca tctgaccatt
    22921 ttaaattatc acgaagtgta gcagtataag taagaccatc agaagaaacg gatacttttt
    22981 tagctaaatc gggttttggt ttgccctctt tatcaattct aagtaaattg ctgcctgaat
    23041 tacctatagc taagtttgaa taagcatctg tattctttga aatgtccaat gtactgattt
    23101 cagtaggaat ataccaatta ataactttat tatctccaga gttctttgac tcattaccac
    23161 aggcaaccag ggtaactgtg gtggctgtta gagtcgccaa agatataccc aaaaacttaa
    23221 gtttttttct attaaaacta gacataatac cagtcctcct aaaccttttt
//
LOCUS       DATRQA010000014        22774 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.014, whole genome shotgun sequence.
ACCESSION   DATRQA010000014 DATRQA010000000
VERSION     DATRQA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22774
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.014"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            137..1801
                     /locus_tag="VBD27_000417"
     CDS             137..1801
                     /locus_tag="VBD27_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO7024453.1"
                     /translation="MKLSKKLLFSAAVLTMVAGSTVEPVAQFATGMSIVRAAEVSQER
                     PAKTTVNIYKLQADSYKSEITSNGGIENKDGEVISNYAKLGDNVKGLQGVQFKRYKVK
                     TDISVDELKKLTTVEAADAKVGTILEEGVSLPQKTNAQGLVVDALDSKSNVRYLYVED
                     LKNSPSNITKAYAVPFVLELPVANSTGTGFLSEINIYPKNVVTDEPKTDKDVKKLGQD
                     DAGYTIGEEFKWFLKSTIPANLGDYEKFEITDKFADGLTYKSVGKIKIGSKTLNRDEH
                     YTIDEPTVDNQNTLKITFKPEKFKEIAELLKGMTLVKNQDALDKATANTDDAAFLEIP
                     VASTINEKAVLGKAIENTFELQYDHTPDKADNPKPSNPPRKPEVHTGGKRFVKKDSTE
                     TQTLGGAEFDLLASDGTAVKWTDALIKANTNKNYIAGEAVTGQPIKLKSHTDGTFEIK
                     GLAYAVDANAEGTAVTYKLKETKAPEGYVIPDKEIEFTVSQTSYNTKPTDITVDSADA
                     TPDTIKNNKRPSIPNTGGIGTAIFVAIGAAVMAFAVKGMKRRTKDN"
     gene            1890..2813
                     /locus_tag="VBD27_000418"
     CDS             1890..2813
                     /locus_tag="VBD27_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7024454.1"
                     /translation="MKQTLKLMFSFLLMLGTMFGISQTVLAQETHQLTIVHLEARDID
                     RPNPQLEIAPKEGTPIEGVLYQLYQLKSTEDGDLLAHWNSLTITELKKQAQQVFEATT
                     NQQGKATFNQLPDGIYYGLAVKAGEKNRSVSAFLVDLSEDKVIYPKIIWSTGELDLLK
                     VGVDGDTKKPLAGVVFELYEKNGRTPIRVKNGVHSQDIDAAKHLETDSSGHIRISGLI
                     HGDYVLKEIETQSGYQIGQAETAVTIEKSKTVTVTIENKKVPTPKVPSRGGLIPKTGE
                     QQAMALVIIGGILIALALRLLSKHRKHQNKD"
     gene            2815..3732
                     /gene="srtC1"
                     /locus_tag="VBD27_000419"
     CDS             2815..3732
                     /gene="srtC1"
                     /locus_tag="VBD27_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC1"
                     /protein_id="HEO7024455.1"
                     /translation="MGQKSKISLATNIRIWIFRLIFLAGFLVLAFPIVSQVMYFQASH
                     ANINAFKEAVTKIDRVEINRRLELAYAYNASIAGAKTNGEYPALKDPYSAEQKQAGVV
                     EYARMLEVKEQIGHVIIPRINQDIPIYAGSAEENLQRGVGHLEGTSLPVGGESTHAVL
                     TAHRGLPTAKLFTNLDKVTVGDRFYIEHIGGKIAYQVDQIKVIAPDQLEDLYVIQGED
                     HVTLLTCTPYMINSHRLLVRGKRIPYVEKTVQKDSKTFRQQQYLTYAMWVVVGLILLS
                     LLIWFKKTKQKKRRKNEKAASQNSHNNSK"
     gene            3689..4540
                     /gene="srtC2"
                     /locus_tag="VBD27_000420"
     CDS             3689..4540
                     /gene="srtC2"
                     /locus_tag="VBD27_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC2"
                     /protein_id="HEO7024456.1"
                     /translation="MKKRLVKIVTIIRNNKIRTLIFVMGSLILLFPIVSQVSYYLASH
                     QNINQFKREVAKIDTNTVERRIALANAYNETLSRNPLLIDPFTSKQKEGLREYARMLE
                     VHEQIGHVAIPSIGVDIPIYAGTSETVLQKGSGHLEGTSLPVGGLSTHSVLTAHRGLP
                     TARLFTDLNKVKKGQIFYVTNIKETLAYKVVSIKVVDPTALSEVKIVNGKDYITLLTC
                     TPYMINSHRLLVKGERIPYDSTEAEKHKEQTVQDYRLSLVLKILLVLLIGLFIVIMMR
                     RWMQHRQ"
     gene            4622..7294
                     /locus_tag="VBD27_000421"
     CDS             4622..7294
                     /locus_tag="VBD27_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO7024457.1"
                     /translation="MKKRQKIWRGLSVTLLILSQIPFGILVQGETQDTNQALGKVIVK
                     KTGDNATPLGKATFVLKNDNDKSETSHETVEGSGEATFENIKPGDYTLREETAPIGYK
                     KTDKTWKVKVADNGATIIEGMDADKAEKRKEVLNAQYPKSAIYEDTKENYPLVNVEGS
                     KVGEQYKALNPINGKDGRREIAEGWLSKKITGVNDLDKNKYKIELTVEGKTTVETKEL
                     NQPLDVVVLLDNSNSMNNERANNSQRALKAGEAVEKLIDKITSNKDNRVALVTYASTI
                     FDGTEATVSKGVADQNGKALNDSVSWDYHKTTFTATTHNYSYLNLTNDANEVNILKSR
                     IPKEAEHINGDRTLYQFGATFTQKALMKANEILETQSSNARKKLIFHVTDGVPTMSYA
                     INFNPYISTSYQNQFNSFLNKIPDRSGILQEDFIINGDDYQIVKGDGESFKLFSDRKV
                     PVTGGTTQAAYRVPQNQLSVMSNEGYAINSGYIYLYWRDYNWVYPFDPKTKKVSATKQ
                     IKTHGEPTTLYFNGNIRPKGYDIFTVGIGVNGDPGATPLEAEKFMQSISSKTENYTNV
                     DDTNKIYDELNKYFKTIVEEKHSIVDGNVTDPMGEMIEFQLKNGQSFTHDDYVLVGND
                     GSQLKNGVALGGPNSDGGILKDVTVTYDKTSQTIKINHLNLGSGQKVVLTYDVRLKDN
                     YISNKFYNTNNRTTLSPKSEKEPNTIRDFPIPKIRDVREFPVLTISNQKKMGEVEFIK
                     VNKDKHSESLLGAKFQLQIEKDFSGYKQFVPEGSDVTTKNDGKIYFKALQDGNYKLYE
                     ISSPDGYIEVKTKPVVTFTIQNGEVTNLKADPNANKNQIGYLEGNGKHLITNTPKRPP
                     GVFPKTGGIGTIVYILVGSTFMILTICSFRRKQL"
     gene            7348..8138
                     /locus_tag="VBD27_000422"
                     /pseudo
     CDS             7348..8138
                     /locus_tag="VBD27_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            8387..8992
                     /locus_tag="VBD27_000423"
     CDS             8387..8992
                     /locus_tag="VBD27_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO7024458.1"
                     /translation="MARDPYSDRSRYLDPKVLSSSFGAFFPADNIKVAWSNNSSTLFT
                     PPINANYTTQIQAIGTTIKSQIPESILTVTDKKSRKFSINKIDEAKEGLVGATFTLSK
                     RTTVAADHQVQGDFIPVSKETTVGRTTLTFDNLKPGFYDLKETKAPNAYVLDPKTYVV
                     VVQNSGKTTIVDEANFKEADYPMADNTSHVECVALLQRSKG"
     gene            10090..10266
                     /locus_tag="VBD27_000424"
     CDS             10090..10266
                     /locus_tag="VBD27_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024459.1"
                     /translation="MHVGKSYFDYQENALIPQLGFLYSKLTKKIELDNRLSPTEQKLL
                     ITLLLHTKGLIIDM"
     gene            11611..11880
                     /locus_tag="VBD27_000425"
     CDS             11611..11880
                     /locus_tag="VBD27_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024460.1"
                     /translation="MVSLLLLKVKQEELIEFHNVFYREAISSYLETENFEKAKVTEEN
                     YQVFLGIRTYQKSYNYQDHSENLKGNIEVAMIGVENNISDEHIFC"
     gene            11941..13092
                     /locus_tag="VBD27_000426"
     CDS             11941..13092
                     /locus_tag="VBD27_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7024461.1"
                     /translation="MVRKFLKSLLSLFLIAVIATGISVACFFFIPENKGNITPILLHR
                     FMRKNNVNGMMIVSDNTGKPITISHGINRGEVETDIENNKLFPMASLQKLMTGIIIQR
                     LIDQDVLSEDDRLSQFFPQVKGSNSITIHQLLTHTSGLREKGVKVSPYLKNEREQLQF
                     CLKHYNFVNKKSWYYSNINFSFLTGIATQVTGRTYAELVDDVIKNPLRLDDTQSYQSV
                     VNHDLVSPMRKNGKLNKINIFNQVSTAYGAGDFFTTPLNFWVLMRSFSKGYFFPTDEY
                     TKHQNDAISHYYGGLYMHGRIVNSNGAFFQYSSFGYADLKTHDTMVLFMNNKVYSDAS
                     HVAPKAFEYYQKFMFLNKIFHLVFYFVFSFFLMLLIRHSFRKRRYKKRL"
     gene            13292..14284
                     /locus_tag="VBD27_000427"
     CDS             13292..14284
                     /locus_tag="VBD27_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7024462.1"
                     /translation="MSMIEVSHLQKNFIKTVKAPGLKGAFQSFLRPEKHTFEAVKDLT
                     FDVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRIDGKLPQENRQNYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDKEFRKRMAFLNEVLELNDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCHRIFMIDRGQEIFDGTVSQLKETFGKMKTLSFDLRPGQEHISSSLIGKSEIN
                     IKRNDLVLDIQYDSSRYQTADIIQQTLADFSVRDLKMTDADIEDIIRRFYRNEL"
     gene            14287..15105
                     /locus_tag="VBD27_000428"
     CDS             14287..15105
                     /locus_tag="VBD27_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO7024463.1"
                     /translation="MNRIWRRYKPFISTEIQGLITYRVDFILYRIGDVIGAFVAFYLW
                     KAVFDSSSQSLIQGFQLSDMILYIIMSFVTNLLTRTDSSFMIGDEVKDGSIIMRLLRP
                     VHFAASYLFMEIGSRWLIFLSIGVPFLLVITGVRLFLGTDLIQAIVLVVFYIISIILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFMSGSLIPLTFFPKIVADILGFLPF
                     SSLIYTPVMIIIGKYDGSQIVQALLLQIFWLIVMVALSQLIWKKVQSHITIQGG"
     gene            15107..15892
                     /locus_tag="VBD27_000429"
     CDS             15107..15892
                     /locus_tag="VBD27_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7024464.1"
                     /translation="MTKYQRMHFIFIKQYMKQIMEYKIDFFVGVLGVFLTQGLNLLFL
                     NVLFQRIPSLEGWTFQQIAFIYGFSLLPKGIDHLFFDNLWALGQRLIRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGFILLSTTVSSISWIVPKVLLFIFIIPFATLIYTS
                     LKIATSSIAFWTKQSGAVIYIFYMFNDFAKYPVAIYNNLLRWIISFVIPFAFTAYYPA
                     AYFLQDRNVYFNIGGVILISLISFMVSLILWHKGVEVYESAGS"
     gene            16517..16822
                     /locus_tag="VBD27_000430"
     CDS             16517..16822
                     /locus_tag="VBD27_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylX protein"
                     /protein_id="HEO7024465.1"
                     /translation="MGRSNILSRCEQCSDNQCGLYKTRLKDKLLLVVQLVGNIRYSEF
                     LTEGGVYHKGETLLEVITLGISYEYKSYGDIKVIKKYCADGSVVDYNMPILLMERIE"
     gene            16822..17670
                     /locus_tag="VBD27_000431"
     CDS             16822..17670
                     /locus_tag="VBD27_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HEO7024466.1"
                     /translation="MKTVIIYGGQGNVQSKHLCQLVKTPKGEILFSKLQATDLESYQL
                     MMDVINHKVSLDNVHASMLANFLYNDWLTNDEKIVSGIGFSAHSAGIFNVLYASGSTS
                     FENIIHFIKRRAQLIENCHVSEELWLLMVENIDMFSKDVLKEQSEKVKLAIITDDRAG
                     VIAMDQVNRSFFDDVMTNKGYFYKLKQLGVKAPYHTDFLNESKEQYKQLIESLDISQN
                     DDYDYIFHCDQLVDEILYQWTNLFNWRAIKETIIEKDAEIIDISPNRFISSQLQKMKK
                     RGRKQL"
     gene            17667..18389
                     /gene="fabG"
                     /locus_tag="VBD27_000432"
     CDS             17667..18389
                     /gene="fabG"
                     /locus_tag="VBD27_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEO7024467.1"
                     /translation="MKVAIVTGGTRGIGRAITKELYKEGYKVIAIYNSNDAKARALQE
                     ELPKLDVYKCNISDAKAVQKLVTKIFREYGGIDCLVNNAGIVRDGFFLMMSKEKWMDV
                     ININIMGLVNMSKAVLKIMKAKRIQGKVINISSTSGIAGQIGQANYSATKGAIISITK
                     TLAKEFASDGITINCVSPGFIETDMTNELQNKEELKEHLIPLKRFGQPEEVAWLVSFL
                     ASEKANYITGKNIVIDGGMIND"
     gene            18382..18687
                     /locus_tag="VBD27_000433"
     CDS             18382..18687
                     /locus_tag="VBD27_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7024468.1"
                     /translation="MINWAEERKIIKTRQEVVDEIKGILIDSLMLDIDKDLIMNDQPL
                     FGRGLELDSIDALELSIGISTTFGVELNDDDISILSSVNRLADFVIENSEDIDDQAK"
     gene            18671..19147
                     /gene="fabZ"
                     /locus_tag="VBD27_000434"
     CDS             18671..19147
                     /gene="fabZ"
                     /locus_tag="VBD27_000434"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO7024469.1"
                     /translation="MTKLSSEEIIKMLPHRYPFLMVDRVQSYKKGIIECRKNITINEP
                     YFQGHFPQRPIVPGVLMIEMAAQSAALLYILDALDGSLPSLDELTAEKIAEKVGYLAS
                     VKNFKFKKIVTPGDQLNITCKSQSKLGHLLEIQVVIRDENKKEVASGRMLVSENGN"
     gene            19137..20066
                     /locus_tag="VBD27_000435"
     CDS             19137..20066
                     /locus_tag="VBD27_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7024470.1"
                     /translation="MEIKLKNIGKKYGSFEALRDINLIFEEGKFYGLLGPNGAGKTTL
                     FNLLIQNFKQTSGDIKWEVDGKPLSIKDFYRHIGIVFQSNRLDDNLTVEENLISRGAL
                     YGLSKSQVRNRLKDLQTYLDITAIKKQKYGSLSGGQKRKVDIARALLPQPSLLLLDEP
                     TTGLDPQSRRDLWDAIAQLNQQSQMTVVLITHYLEEMSACDVLNVLIEGNIYYSGDIK
                     SFIEQHSTTNLNVVLKPEKSLDQLSIADFVNKCQVLSEREIVFKDISVEEMMQVISDN
                     QDKSIIETFNVEYSNLEAAYLNLLREKERKNNV"
     gene            20059..20937
                     /locus_tag="VBD27_000436"
     CDS             20059..20937
                     /locus_tag="VBD27_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7024471.1"
                     /translation="MFELISRNRKVYTRDRLAFFMSFLSVIILILVYQIFLGKIQLDA
                     IKAALRSNTVSSDTIHMVNYWLIAGLTTIISMTSTLGAFGVMVSDKEKKLNEDFKVSP
                     ISNIKIELSYAIFAVLFGIVMTMFSCIFAIGIFNGFNSLLEYSAMDYLTILGIVSLGT
                     VLSAAMILPILAFIHTSSAFTTLSTIVGTFIGFISGVYLSIGSVGKALQQVMTWFPLT
                     QINSLLKQVLMKGSIAKVFDKANEATVSNYKESYGVVLRNADGERLSNHFMLIYIIAL
                     ILILLAIHFIIKKVKK"
     gene            20934..>22774
                     /locus_tag="VBD27_000437"
     CDS             20934..>22774
                     /locus_tag="VBD27_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylE protein"
                     /protein_id="HEO7024472.1"
                     /translation="MKDDNKLKISEASLEDYSEVVHLFNRNHVYQFPDGRPLTVDDLD
                     LTLKVKEVTHLFLLKNHGVLIGTSAFFKFITYGCLDWNSSFSGFLLIDSKSRSGQAIT
                     YLYKTILKKITKLKFSNIYTEISNYNKPSLALSKLNGFKEYDKTYEDILHCRSLRSHL
                     PKILNTFRISNYYGKTYDISTFQIMEEIENPLEEETEIRTKVSDEEILFKAEDSASLP
                     YYLKMSLFQMEIAKLDNRYVLQVDFLSEQVKRVRVKTGKYHLANLTRAHPSLTLSRFA
                     NYYYIQATVETLYGNIDVQLERRKKHYRDATICLKRTFQGYDLLISPNGSLIFEKQKR
                     KILEDSFLIFSQPLDKKLVVKEEENHITIKCFYQGALIEKIMTFTSDEEITCVYKCNQ
                     KAKEMFPKLLKQTFKLHCQEQLIRDSEGYLVNVPGSYPIEHDDFLRADKFEDRQFHYY
                     LPNEDKMISYSPPGKASNQMQFRPLCLIDTDSLSFPLTYHFRISQASSEEALINLKKQ
                     PIWDSNYQASATDLLKHISNLTLEEEKDYGIKRMIANRKHYPSHKLVLAYNQIVLPKN
                     EIPRDSELYSISFDYRIRGKFVQIRQGEHVKYDNKSYVLENSQQLVL"
BASE COUNT         7724 a         3447 c         4438 g         7165 t
ORIGIN      
        1 aaaaatagta aaaataaaca cgaatttaaa ataagcaaat tttttaagaa aatctgtgct
       61 aaactttaat agttttgtgc ttaataataa tcagcactta caaagaacaa agggaaaagc
      121 gaggagagaa cttttaatga aattatcgaa gaagttattg ttttcggctg ctgttttaac
      181 aatggtggcg gggtcaactg ttgaaccagt agctcagttt gcgactggaa tgagtattgt
      241 aagagctgca gaagtgtcac aagaacgccc agcgaaaaca acagtaaata tctataaatt
      301 acaagctgat agttataaat cggaaattac ttctaatggt ggtatcgaga ataaagacgg
      361 cgaagtaata tctaactatg ctaaacttgg tgacaatgta aaaggtttgc aaggtgtaca
      421 gtttaaacgt tataaagtca agacggatat ttctgttgat gaattgaaaa aattgacaac
      481 agttgaagca gcagatgcaa aagttggaac gattcttgaa gaaggtgtca gtctacctca
      541 aaaaactaat gctcaaggtt tggtcgtcga tgctctggat tcaaaaagta atgtgagata
      601 cttgtatgta gaagatttaa agaattcacc ttcaaacatt accaaagctt atgctgtacc
      661 gtttgtgttg gaattaccag ttgctaactc tacaggtaca ggtttccttt ctgaaattaa
      721 tatttaccct aaaaacgttg taactgatga accaaaaaca gataaagatg ttaaaaaatt
      781 aggtcaggac gatgcaggtt atacgattgg tgaagaattc aaatggttct tgaaatctac
      841 aatccctgcc aatttaggtg actatgaaaa atttgaaatt actgataaat ttgcagatgg
      901 cttgacttat aaatctgttg gaaaaatcaa gattggttcg aaaacactga atagagatga
      961 gcactacact attgatgaac caacagttga taaccaaaat acattaaaaa ttacgtttaa
     1021 accagagaaa tttaaagaaa ttgctgagct acttaaagga atgacccttg ttaaaaatca
     1081 agatgctctt gataaagcta ctgcaaatac agatgatgcg gcatttttgg aaattccagt
     1141 tgcatcaact attaatgaaa aagcagtttt aggaaaagca attgaaaata cttttgaact
     1201 tcaatatgac catactcctg ataaagctga caatccaaaa ccatctaatc ctccaagaaa
     1261 accagaagtt catactggtg ggaaacgatt tgtaaagaaa gactcaacag aaacacaaac
     1321 actaggtggt gctgagtttg atttgttggc ttctgatggg acagcagtaa aatggacaga
     1381 tgctcttatt aaagcgaata ctaataaaaa ctatattgct ggagaagctg ttactgggca
     1441 accaatcaaa ttgaaatcac atacagacgg tacgtttgag attaaaggtt tggcttatgc
     1501 agttgatgcg aatgcagagg gtacagcagt aacttacaaa ttaaaagaaa caaaagcacc
     1561 agaaggttat gtaatccctg ataaagaaat cgagtttaca gtatcacaaa catcttataa
     1621 tacaaaacca actgacatca cggttgatag tgctgatgca acacctgata caattaaaaa
     1681 caacaaacgt ccttcaatcc ctaatactgg tggtattggt acggctatct ttgtcgctat
     1741 cggtgctgcg gtgatggctt ttgctgttaa ggggatgaag cgtcgtacaa aagataacta
     1801 aataaaaggc tacttcttaa gtaaccatgt ttaagaaaag agaaatagcc ttatttctct
     1861 ttttgtcgtt tttaaaataa aggaacatca tgaaacaaac attaaaactt atgttttctt
     1921 ttctgttgat gttagggact atgtttggaa ttagccaaac tgttttagcg caagaaactc
     1981 atcagttgac gattgttcat cttgaagcaa gggatattga tcgtccaaat ccacagttgg
     2041 agattgcccc taaagaaggg actccaattg aaggagtact ctatcagttg taccaattaa
     2101 aatcaactga agatggcgat ttgttggcac attggaattc cctaactatc acagaattga
     2161 aaaaacaggc gcagcaggtt tttgaagcca ctactaatca acaaggaaag gctacattta
     2221 accaactacc agatggaatt tattatggtc tggcggttaa agccggtgaa aaaaatcgta
     2281 gtgtctcagc tttcttggtt gacttgtctg aggataaagt gatttatcct aaaatcatct
     2341 ggtccacagg tgagttggac ttgcttaaag ttggtgtgga tggtgatacc aaaaaaccac
     2401 tagcaggcgt tgtctttgaa ctttatgaaa agaatggtag gactcctatt cgtgtgaaaa
     2461 atggggtgca ttctcaagat attgacgctg caaaacattt agaaacagat tcatcagggc
     2521 atatcagaat ttccgggctc atccatgggg actatgtctt aaaagaaatc gagacacagt
     2581 caggatatca gatcggacag gcagagactg ctgtgactat tgaaaaatca aaaacagtaa
     2641 cagtaacgat tgaaaataaa aaagttccga cacctaaagt gccatctcga ggaggtctta
     2701 ttcccaaaac aggtgagcaa caggcaatgg cacttgtaat tattggtggt attttaattg
     2761 ctttagcctt acgattacta tcaaaacatc ggaaacatca aaataaggat tagcatggga
     2821 caaaaatcaa aaatatctct agctacgaat attcgtatat ggatttttcg tttaattttc
     2881 ttagcgggtt tccttgtttt ggcatttccc atcgttagtc aggtcatgta ctttcaagcc
     2941 tctcacgcca atattaatgc ttttaaagaa gctgttacca agattgaccg ggtggagatt
     3001 aatcggcgtt tagaacttgc ttatgcttat aacgccagta tagcaggtgc caaaactaat
     3061 ggcgaatatc cagcgcttaa agacccctac tctgctgaac aaaagcaggc aggggtcgtt
     3121 gagtacgccc gcatgcttga agtcaaagaa caaataggtc atgtgattat tccaagaatt
     3181 aatcaggata tccctattta cgctggctct gctgaagaaa atcttcagag gggcgttgga
     3241 catttagagg ggaccagtct tccagtcggt ggtgagtcaa ctcatgccgt tctaactgcc
     3301 catcgagggc taccaacggc caagctattt accaatttag acaaggtaac agtaggtgac
     3361 cgtttttaca ttgaacacat cggcggaaag attgcttatc aggtagacca aatcaaagtt
     3421 atcgcccctg atcagttaga ggatttgtac gtgattcaag gagaagatca cgtcacccta
     3481 ttaacttgca caccttatat gataaatagt catcgcctcc tcgttcgagg caagcgaatt
     3541 ccttatgtgg aaaaaacagt gcagaaagat tcaaagacct tcaggcaaca acaataccta
     3601 acctatgcta tgtgggtagt cgttggactt atcttgctgt cgcttctcat ttggtttaaa
     3661 aagacgaaac agaaaaagcg gagaaagaat gaaaaagcgg ctagtcaaaa tagtcacaat
     3721 aattcgaaat aataaaatta gaaccctcat ttttgtgatg ggaagtctga ttctcttatt
     3781 tccgattgtg agccaggtaa gttactacct tgcttcgcat caaaatatta atcaatttaa
     3841 gcgggaagtc gctaagattg atactaatac ggttgaacga cgcatcgctt tagctaatgc
     3901 ttacaatgag acgttatcaa ggaatccctt gcttatagac ccttttacca gtaagcaaaa
     3961 agaaggtttg agagagtatg ctcgtatgct tgaagttcat gagcaaatag gtcatgtggc
     4021 aatcccaagt attggggttg atattccaat ttatgctgga acatccgaaa ctgtgcttca
     4081 gaaaggtagt gggcatttgg agggaaccag tcttccagtg ggaggtttgt caacccattc
     4141 agtactaact gcccaccgtg gcttgccaac agctaggcta tttaccgact taaataaagt
     4201 taaaaaaggc cagattttct atgtgacgaa catcaaggaa acacttgcct acaaagtcgt
     4261 gtctatcaaa gttgtggatc caacagcttt aagtgaggtt aagattgtca atggtaagga
     4321 ttatataacc ttgctgactt gcacacctta catgatcaat agtcatcgtc tcttggtaaa
     4381 aggagagcgt attccttatg attctaccga ggcggaaaag cacaaagaac aaaccgtaca
     4441 agattatcgt ttgtcactag tgttgaagat actactagta ttattaattg gactcttcat
     4501 cgtgataatg atgagaagat ggatgcaaca tcgtcaataa cgatgttgtg aatggcttac
     4561 ttacttatca aataggtgac taatgatgat tgtgaataat ggttatctag aagggagaaa
     4621 aatgaaaaag agacaaaaaa tatggagagg gttatcagtt actttactaa tcctgtccca
     4681 aattccattt ggtatattgg tacaaggtga aacccaagat accaatcaag cacttggaaa
     4741 agtaattgtt aaaaaaacgg gagacaatgc tacaccatta ggcaaagcga cttttgtgtt
     4801 aaaaaatgac aatgataagt cagaaacaag tcacgaaacg gtagagggtt ctggagaagc
     4861 aacctttgaa aacataaaac ctggagacta cacattaaga gaagaaacag caccaattgg
     4921 ttataaaaaa actgataaaa cctggaaagt taaagttgca gataacggag caacaataat
     4981 cgagggtatg gatgcagata aagcagagaa acgaaaagaa gttttgaatg cccaatatcc
     5041 aaaatcagct atttatgagg atacaaaaga aaattaccca ttagttaatg tagagggttc
     5101 caaagttggt gaacaataca aagcattgaa tccaataaat ggaaaagatg gtcgaagaga
     5161 gattgctgaa ggttggttat caaaaaaaat tacaggggtc aatgatctcg ataagaataa
     5221 atataaaatt gaattaactg ttgagggtaa aaccactgtt gaaacgaaag aacttaatca
     5281 accactagat gtcgttgtgc tattagataa ttcaaatagt atgaataatg aaagagccaa
     5341 taattctcaa agagcattaa aagctgggga agcagttgaa aagctgattg ataaaattac
     5401 atcaaataaa gacaatagag tagctcttgt gacatatgcc tcaaccattt ttgatggtac
     5461 tgaagcgacc gtatcaaagg gagttgccga tcaaaatggt aaagcgctga atgatagtgt
     5521 atcatgggat tatcataaaa ctacttttac agcaactaca cataattaca gttatttaaa
     5581 tttaacaaat gatgctaacg aagttaatat tctaaagtca agaattccaa aggaagcgga
     5641 gcatataaat ggggatcgca cgctctatca atttggtgcg acatttactc aaaaagctct
     5701 aatgaaagca aatgaaattt tagagacaca aagttctaat gctagaaaaa aacttatttt
     5761 tcacgtaact gatggtgtcc ctacgatgtc ttatgccata aattttaatc cttatatatc
     5821 aacatcttac caaaaccagt ttaattcttt tttaaataaa ataccagata gaagtggtat
     5881 tctccaagag gattttataa tcaatggtga tgattatcaa atagtaaaag gagatggaga
     5941 gagttttaaa ctgttttcgg atagaaaagt tcctgttact ggaggaacga cacaagcagc
     6001 ttatcgagta ccgcaaaatc aactctctgt aatgagtaat gagggatatg caattaatag
     6061 tggatatatt tatctctatt ggagagatta caactgggtc tatccatttg atcctaagac
     6121 aaagaaagtt tctgcaacga aacaaatcaa aactcatggt gagccaacaa cattatactt
     6181 taatggaaat ataagaccta aaggttatga catttttact gttgggattg gtgtaaacgg
     6241 agatcctggt gcaactcctc ttgaagctga gaaatttatg caatcaatat caagtaaaac
     6301 agaaaattat actaatgttg atgatacaaa taaaatttat gatgagctaa ataaatactt
     6361 taaaacaatt gttgaggaaa aacattctat tgttgatgga aatgtgactg atcctatggg
     6421 agagatgatt gaattccaat taaaaaatgg tcaaagtttt acacatgatg attacgtttt
     6481 ggttggaaat gatggcagtc aattaaaaaa tggtgtggct cttggtggac caaacagtga
     6541 tgggggaatt ttaaaagatg ttacagtgac ttatgataag acatctcaaa ccatcaaaat
     6601 caatcatttg aacttaggaa gtggacaaaa agtagttctt acctatgatg tacgtttaaa
     6661 agataactat ataagtaaca aattttacaa tacaaataat cgtacaacgc taagtccgaa
     6721 gagtgaaaaa gaaccaaata ctattcgtga tttcccaatt cccaaaattc gtgatgttcg
     6781 tgagtttccg gtactaacca tcagtaatca gaagaaaatg ggtgaggttg aatttattaa
     6841 agttaataaa gacaaacatt cagaatcgct tttgggagct aagtttcaac ttcagataga
     6901 aaaagatttt tctgggtata agcaatttgt tccagaggga agtgatgtta caacaaagaa
     6961 tgatggtaaa atttatttta aagcacttca agatggtaac tataaattat atgaaatttc
     7021 aagtccagat ggctatatag aggttaaaac gaaacctgtt gtgacattta caattcaaaa
     7081 tggagaagtt acgaacctga aagcagatcc aaatgctaat aaaaatcaaa tcgggtatct
     7141 tgaaggaaat ggtaaacatc ttattaccaa cactcccaaa cgcccaccag gtgtttttcc
     7201 taaaacaggg ggaattggta caattgtcta tatattagtt ggttctactt ttatgatact
     7261 taccatttgt tctttccgtc gtaaacaatt gtaaggtgtc gttgaaatta ttaaataata
     7321 gaaaatgact agttttggcc tttccctatt gtcagtcaga ttagttatta tcaagcttct
     7381 catgccaata ttaatgcctt taaaagaaca gttacaacca ttgaccgtac ggagattcaa
     7441 cgacggttag gtctggctaa agcctacaat gctagtattt ctggaacaag tagtcagtcg
     7501 actcaatctg tgctgagaga ttcttattct gaggagcaaa aaaggcaggg tggactgaat
     7561 acgctaggat gttagaagtc agagagcagg ttgaccatgt gatgattcca aaaatcaatc
     7621 aggatttacc aatctacgct ggttcagaag aggacaatct gcaatgggga gttggtcatc
     7681 tagaagggat aagtttgccg attggagggg cttctacaca tgcggtcttg agcggtcaaa
     7741 gaggtatgcc agctgctcgg ttgtttgcgg atttggataa gatgaaaaaa ggtgattatt
     7801 tttatgttac caatctgaaa gaaaccttgg cttatcaagt ggatcgtatc atggtgattg
     7861 aacctagcca attggatgcc gtgagcattg aagaggataa agattatgtt acccttctga
     7921 cctgtacacc ttatatgggc tctttgtcaa ctgtaatggg tgacttatca ttaacaacga
     7981 gagagaatca gcttggttct ctctcttttt ggatgttcaa agcgatgaga attttgcttt
     8041 taaaattttt aaagttgcga aagccaaagg cttgtcgctt gatgtctttg ataagcttgt
     8101 tggtcgcttc taatttggca ttggaaaggg atagctgagg gcgtttgtga tgtaagtttt
     8161 gtacttcatg aaggtcctaa agaccatttt aaaagcagaa ttgccagttg ttacgttatc
     8221 atctatgaag ctaaagaatt cagttgttcg cttttcttag aaatgaaaga gtatgagctg
     8281 ataaaggtcg ttataatagc ggagctcatc tgagaaaact tttatacctc aaagtcagtc
     8341 tagctttgat atcatgaagc cattaggagt tattccttat cttttagtgg cgcgcgatcc
     8401 atatagtgat agatcgagat atttagatcc aaaagttcta tcatcctctt ttggcgcctt
     8461 ttttccagca gataatatta aggtagcttg gtctaacaac tccagcactt tatttacacc
     8521 acctattaat gcaaactaca ccactcagat tcaagctatt gggacaacga ttaagtcaca
     8581 aattccggaa tcgattttga cggttacgga taaaaagagc aggaagttca gcattaacaa
     8641 gattgacgaa gctaaagaag gcttagtagg tgcgaccttc accttgtcta aacgcacaac
     8701 agtagcggca gatcatcaag tacaaggaga tttcattcct gtcagcaaag agacgacagt
     8761 cggtcggaca acccttacct ttgacaacct taaacctgga ttttatgacc ttaaagaaac
     8821 gaaagcgccg aatgcttacg tacttgatcc taagacttat gttgtggtcg ttcaaaattc
     8881 aggaaaaacg acaattgtgg atgaagctaa cttcaaagag gctgattacc caatggctga
     8941 taataccagc catgtggagt gcgtagcgtt gctacaacga agcaaagggt aaaaatcctt
     9001 tattttaagc actttttcaa gcattttgtc tttattgaaa agagtgattt taacataaaa
     9061 aaggtattaa aaaacatatt gacgtgaccg tttgttttga agtggcttgc gtagacaaaa
     9121 aaatagatac gtcagataaa tttctggcat tacgagaaca tttttagagt gttctctttt
     9181 tttagtttac ggaggaaaaa tatatatgga aaaacaggat tcacgagttc tcatccattg
     9241 ggaggggaat tctggggaca agctcattga acaccaaacc agcgcaacgg ggtggtacta
     9301 ccaagtcgat cgtagcttta gtcaaccaaa aggggaaccg cccagaatga tccaaaggca
     9361 ctagaaagtg tccgtaatga ttcgatttcg ggcggtgatg atgtcatggg ttatgcttat
     9421 agcaaatgta cttggggagt tgcggcacga attaatcagt gggactgaaa ctcaaaggtt
     9481 gaaatggtga gaagattacc attaccagtt caatgggaaa tggtcaggat tgggttggaa
     9541 cagccgaaag actagatggt gaaactgata cagttccaaa agaaggtact attctctctt
     9601 tttaggaaag tagttatggt tcgtatatag gctacggaac tatatctttc gtcacattac
     9661 atctacagat agtaccatga attttgctta tatgaccaag taaagtgagg atatactaac
     9721 aaatgaaata tttattatcg tatttgtcca ttttatcgaa aagtttgcat attatcatta
     9781 tgtttgataa gatgcaaata taatgatagt aggagctaaa tatggatatt taaaaaatca
     9841 agagtatcct aagtgctttc cattttgaaa ttcaaatata gctaatagtt ctagaacttc
     9901 taattgtttt tcgtcgacga tatgaatttt caatcttaac tgttaggatt ccacctccct
     9961 ttggttaaag aaaaaaggtc aggtcgttta gataactttg tcaaacaagc tcaagctatc
    10021 taaaaatagt ttgaaatggg cattactcta gtttttaata agctatctga tgagcagaag
    10081 gagaagttaa tgcatgttgg gaagtcttat tttgactatc aagaaaatgc tcttatccca
    10141 caattaggtt ttctatattc taaattaact aaaaaaattg aacttgataa tcggttgtct
    10201 ccgactgaac aaaagttatt gattacctta ttattacata ctaaaggttt aatcattgat
    10261 atgtaagaag taagtcagct aaccgatctt tctattctaa aacttatatt gttgctttag
    10321 aaattttaaa gagcgtggat ggcttcataa taaacagaaa tcttaccaat ttgcgaagcc
    10381 aaaaaatatg atatttgaag agtccaaaga tctaatagat agtccagtta gagaagcgtt
    10441 gattataagt gataaggatt ttcaaaaatt aaaacaagag ctattatttt aaccgactta
    10501 ttttaaagac ttatcatatc taggcttgct tgatgattcg gaaaaatacg gagactatac
    10561 tatttcaagg aaaagataca aaagtttcga atcaagtctt caactataca tccttcaaag
    10621 tcatcggcta gagatttgga attatgaacc aatccctttg attactagaa aaataaatag
    10681 cttggagagg ctaacgtgac actggttgat ccaatctcgc tttatttaac actaagaatg
    10741 atgaagaccc tcgtattgaa gaagaagttg agcagctaga agataagatt ttgagaatat
    10801 aatcgttttt tcgtgctaca ctaacttgtc agggttgcaa cagacaaaaa aataatttag
    10861 gagggattcg ttatgcgtac aaagtatata ggatatgaaa ttgttttaag gggtggttat
    10921 taatatggat aagaaaaagg ttgtttcaac tatcgtatct acagggtttc ttatagtttc
    10981 aatatcaaat ttttttagag agctatcacc tatacttttt ctcttgaata ttattggatt
    11041 tttctgcttc ctattattat tttctgccaa cataaataaa ttgttcttaa aacgatagcc
    11101 gttatatagg atatggtatg tatgcatttg gtgaaattat aggtaaaacg aatggaatag
    11161 tacttactcc tcaaagctat cgtgagagat ataaaaggat atcgaggagt cactagatag
    11221 ttggatctca ttgaaaaaat ttgtttaaag tatctcatat aagcataaaa cgaggaatca
    11281 atcaaaattt cttgattgat tccttgtttt ttggctcttt gtcaactgta gtgggttgat
    11341 gaaaaattat attctggaga aaaccaaatt ggttttctcc tttttgatat tcaaagtgat
    11401 atgaattctc tttttaaaat attcaaagtt tctaaagcta aatgtttgtg cttgatgatt
    11461 ttgatgagat tattggttgc tcaagtttgg tgtttgaata aggcgattca aggacattga
    11521 taaccttatc tctgtcttta aaaaatgtcc taaaaacagt ttaaaagatt ggagtgacac
    11581 tagagaaagt gttttaaaca gggtaaagaa atggtgagct tgcttctctt gaaagtaaag
    11641 caggaggagt tgatagagtt tcataatgtt ttttatagag aagcgatatc ttcttatctt
    11701 gaaacagaaa attttgagaa ggctaaagtg actgaagaga attaccaagt ttttttagga
    11761 atccgtactt atcagaagag ttataactat caagatcata gtgagaatct aaaaggaaat
    11821 attgaggtag caatgatagg agttgaaaat aatattagcg acgaacatat tttttgctag
    11881 aatatataaa atgtctttga aataatttag ccctgtgtta tgatattaag gaggttattt
    11941 atggtacgta aatttttaaa atcgctccta tctctctttc ttatagcagt aatcgccacc
    12001 ggtatttcag tggcatgttt cttttttatt ccagaaaata aagggaatat tacaccgatt
    12061 ttattgcatc gctttatgcg aaaaaataat gttaatggaa tgatgattgt tagcgataat
    12121 actggtaaac cgattaccat ctcgcatggg attaaccgtg gggaagttga aacggatatt
    12181 gaaaataata aattatttcc aatggcttct ctccaaaaac tcatgacagg aattattatc
    12241 caaagattaa ttgatcaaga tgtcttgagt gaagacgacc gattgtctca gttctttccc
    12301 caagtaaaag gtagtaatag tattaccatc caccaattgt tgacccatac gagtgggtta
    12361 agagaaaaag gtgttaaggt gtctccttat ctaaaaaatg agagagaaca attacaattt
    12421 tgcttaaaac actataactt tgttaataag aaatcgtggt attattctaa tatcaacttt
    12481 agttttctaa caggtatagc cactcaagtt acgggaagga cctacgctga acttgttgat
    12541 gatgtgataa aaaatccttt gcgattagat gatacccaaa gttaccaatc agttgttaat
    12601 catgacttag ttagcccaat gcgaaagaac ggcaagttaa ataaaattaa tatctttaat
    12661 caagtatcca cagcatatgg agctggagat tttttcacaa ctccgctgaa cttttgggtg
    12721 ttgatgcggt ctttttctaa agggtacttt ttcccaacgg atgaatatac caaacatcaa
    12781 aatgatgcga tatcacatta ttatggtggg ctttacatgc atggtcgtat agttaattct
    12841 aatggggcct ttttccagta ttcaagtttt ggttatgcag atctaaagac gcatgataca
    12901 atggtgcttt ttatgaataa caaagtctat agtgatgcta gtcacgtggc accaaaagca
    12961 tttgaatatt atcaaaaatt tatgttcctt aataagattt tccacttagt cttttacttt
    13021 gtattttctt tctttttaat gcttttaata cgtcattcgt ttaggaaaag aaggtataag
    13081 aaacgtctct aaatcacatg aataaaaaag aaggctaagt tggccttctt ttttattcaa
    13141 tattaagcag taacaccatc taagaaaacg tttgcattga atttttaatt gtgctataat
    13201 aaatagctgt taatcattat tatagtttaa taatctaact gtcgagctga tttaagctat
    13261 aaaaatactg ttgagtaaaa gaggtattaa tatgtcaatg attgaagttt ctcaccttca
    13321 gaaaaatttt ataaaaaccg ttaaagcacc agggttaaaa ggagcatttc aatcatttct
    13381 tcgtcctgaa aaacacacct ttgaagctgt taaagattta acatttgatg tgcctaaagg
    13441 acaaattttg ggatttattg gagcgaacgg tgcaggaaaa tctacaacaa ttaaaatgtt
    13501 aactgggatt ttaaaaccaa cttcgggctt ttgccgtatt gatggcaaat tgccacaaga
    13561 aaaccgacaa aattatgtta aggatattgg agtcgttttt gggcaacgta cgcagttgtg
    13621 gtgggattta gctctacaag aaacctatac agtgttaaaa gaaatctatg acgttcctga
    13681 taaagaattt cgcaaacgca tggctttttt aaatgaggtt ttggaactaa atgactttat
    13741 aaaggatcct gttagaactt tgtctctcgg acagcgtatg agagcggata tcgcagcttc
    13801 tttattacat aacccaaagg ttcttttctt agatgaaccc accattggct tggacgtttc
    13861 agtgaaagat aatatccgac gtgcaatcac acaaattaat caagaagaag agacaacgat
    13921 tttactaacg acacatgacc tcagtgatat tgaacaactt tgtcatcgta tttttatgat
    13981 tgatagagga caagaaatat ttgatggcac agtttctcag ctaaaagaga catttggaaa
    14041 gatgaaaact ctttcttttg atttgcgccc aggacaagag catatcagct ctagtttaat
    14101 tggaaagtcg gagattaata ttaaacgtaa tgacttagtt ttagatattc agtatgatag
    14161 ttctcgctac caaactgctg acataattca gcagacatta gcagattttt ctgtacgtga
    14221 ccttaaaatg acagatgcgg atattgaaga tatcattcgt cgcttctata gaaatgagct
    14281 ataaggatga acagaatttg gagacgttat aaacctttta ttagtacaga aatccaagga
    14341 ttaatcacat atcgtgttga tttcatcctt tataggatag gtgatgtgat aggagcattt
    14401 gttgcatttt atctatggaa agcagttttc gattcctcaa gtcaatcctt aatccaaggt
    14461 tttcagctgt cagatatgat tctctatatt attatgagtt ttgtgactaa tttattaaca
    14521 agaactgata gttcatttat gataggtgac gaagtaaaag acggttcaat tattatgcga
    14581 ctcttacgtc cagttcactt tgctgcttcc taccttttca tggagattgg ttctagatgg
    14641 ctcatctttt taagtatagg ggtaccgttt ttgcttgtta ttacgggggt acgcctcttc
    14701 ttggggactg atttgattca agctatagtg ttagtggtat tttatattat tagcattatt
    14761 ttagcttttt tgattaactt cttttttaat atttgttttg gcttctcggc atttgttttt
    14821 aaaaatttat gggggtcaaa cctgttaaaa aattcgttag tcgcatttat gtcggggagc
    14881 ttgattcctc ttactttttt tccgaaaatc gttgctgata ttttaggatt tttaccattt
    14941 tcatctttaa tttacacacc agtaatgatt attattggta aatatgatgg cagtcaaatt
    15001 gtgcaagcct tacttttaca aatattttgg ctaatagtaa tggtagcttt atctcaatta
    15061 atatggaaaa aagtacagtc gcatatcact atccaaggag gttgagatga caaaatatca
    15121 gcgaatgcac tttattttta ttaaacaata catgaagcaa atcatggagt ataaaataga
    15181 cttttttgta ggcgttttag gagtattttt aacacaaggg ctaaatcttt tatttctgaa
    15241 tgttcttttt caacgtatcc cttctttaga aggatggact tttcagcaaa tagcttttat
    15301 ttatggtttt tctctcctcc caaaaggaat tgatcacctt ttctttgata acttgtgggc
    15361 tcttggtcaa aggcttattc gaaaaggaga atttgataag tatttaacca ggccgatcag
    15421 tcctttattt catgtcctag ttgaaacctt tcaagtagat gcattggggg agttattggt
    15481 tggttttatt ctgttatcta ccacagtatc tagtataagc tggatcgtgc caaaagtatt
    15541 attatttata tttattatcc cctttgcaac tttgatttat acctccttga agattgctac
    15601 gtcaagtatc gctttttgga ccaaacaatc tggtgctgtt atctatattt tttatatgtt
    15661 taatgacttt gctaagtatc ctgtcgcaat atataataat ctattgcgtt ggatcattag
    15721 ttttgtcatt ccatttgctt ttacagctta ttatcccgct gcttacttct tacaagatag
    15781 aaatgtctat tttaatattg gtggggttat cttgatttcc ttgatttctt ttatggtatc
    15841 actaatactt tggcataaag gagtagaagt atatgagagt gcgggttctt gattagtaag
    15901 gcaagccatt aatttttttg agtgtttaga agttttaggc ttactaactt agctttgtta
    15961 gtgatgatat actcccctta tagtgtctag tgaatttttg tttttttact gtccactata
    16021 aggggagtat atcaggtgaa gagggactat tagtgttgag aataatgtcc ctcatagaac
    16081 aataattacc atatctttac caaaataaag ttaaaaacgc tcttagatgt gctttctaag
    16141 agcgtttttt ggtctataaa aggtaagcaa aaagcagatg tcatttatga aagtgttgac
    16201 aaagatgaaa tactttgtta ctattttaac ataataatga tattttaatt agagtgtgaa
    16261 tttataatta atttttacaa tgtttatttt aaatttaaac taatgtttat agcagtgttt
    16321 tactcatgat tttgtgaatt caacacttaa gtcatgacct cattttaaag agtttcagac
    16381 gacacagaaa ggaagaaaac ataagaaagg caatgcacgg cttgtccata gtagtgtttg
    16441 aagtagatgt ttagaaatat tttattttcc aacagtactg atatcagagc gaagatgatt
    16501 agttgaaggt gataatatgg gacgttctaa tattttgagt aggtgtgaac aatgttctga
    16561 taatcaatgt ggcttgtata aaacgcggct taaagacaag ttactattag tagtccaact
    16621 ggttggaaat attaggtatt ctgagtttct tacggaaggt ggtgtatatc ataagggaga
    16681 aaccttgttg gaagttataa ccttaggaat ttcttatgaa tataaaagtt atggggatat
    16741 caaagtaatc aagaagtact gtgctgatgg cagtgtcgtt gattataaca tgccaatact
    16801 attaatggaa aggattgaat aatgaaaaca gtaataatat atggaggaca gggtaatgtt
    16861 cagtcaaaac acctttgtca actagttaaa actccaaaag gtgaaattct tttttcaaag
    16921 ttacaagcaa ctgatttaga atcatatcag ttaatgatgg atgtcattaa ccacaaggta
    16981 agtttagaca atgttcatgc gtctatgtta gcaaattttt tatataatga ttggctaaca
    17041 aacgatgaaa aaatagtatc tgggatagga ttttcagctc atagcgccgg tattttcaat
    17101 gtgttatatg ctagtggtag tactagtttt gaaaacatta ttcactttat taaacgtcgt
    17161 gcccaactta tagaaaattg tcatgtgtct gaagagcttt ggcttttgat ggttgaaaac
    17221 attgatatgt ttagtaagga tgttttaaaa gaacagtctg agaaagtaaa attagctatt
    17281 attactgatg atagagctgg tgtaattgct atggatcaag tcaaccgttc tttctttgat
    17341 gacgtgatga ctaacaaagg ttatttttat aaattaaagc agttaggcgt aaaagcacca
    17401 tatcatactg attttttaaa tgaatctaaa gagcagtata aacaattgat tgaaagccta
    17461 gatatttcgc aaaatgatga ctatgactat atttttcatt gtgatcaatt ggtagatgaa
    17521 attttatatc agtggaccaa tctctttaac tggcgtgcga ttaaggaaac tatcatagaa
    17581 aaggatgcgg agattattga tatttctcct aataggttta tttctagtca gttgcaaaaa
    17641 atgaaaaaga gggggaggaa acagttatga aagtagcaat agttactggt ggtaccagag
    17701 gaattggaag ggctattaca aaagagttat acaaagaagg ttacaaagtc attgctattt
    17761 ataatagcaa tgatgctaag gcaagagctt tacaagagga gttaccaaaa ttagacgttt
    17821 ataaatgcaa tatttcagat gcaaaagcag tacaaaaatt ggtgactaaa atttttagag
    17881 aatatggcgg tattgattgt ttagttaata atgctggtat tgttagagat ggctttttct
    17941 tgatgatgtc taaggaaaaa tggatggatg tcattaacat aaatattatg gggctagtta
    18001 acatgagtaa agccgtgtta aaaatcatga aggctaagcg tatacaggga aaggttatca
    18061 atatttcttc aactagtggt atagcagggc agatagggca agcgaactat tcagcaacga
    18121 aaggcgctat catttccatt actaaaactc tagcgaaaga atttgcatca gatggtatta
    18181 cgattaattg tgtaagtcca ggattcattg aaacagatat gacaaatgaa ttgcaaaata
    18241 aagaagagct aaaagagcat cttattcctc taaaaaggtt tggacaacct gaagaggtgg
    18301 cttggctcgt aagcttttta gcaagtgaga aggcaaacta tattacagga aaaaatattg
    18361 tgattgatgg aggaatgatt aatgattaac tgggctgaag aacgtaaaat aataaaaaca
    18421 cgtcaagaag tagttgatga aattaaaggt attttgattg actctttaat gttggatatt
    18481 gataaagatc tgattatgaa tgatcaacca ttatttggtc gtggattaga gctagactct
    18541 attgatgccc tagaactgtc aatagggatt tcaacaacgt ttggtgttga actaaatgat
    18601 gatgatattt caatattatc atcagtaaat agactagcag attttgtgat tgaaaatagt
    18661 gaggatatag atgaccaagc taagtagtga agaaattata aaaatgttac ctcatcgcta
    18721 tccgtttcta atggtggata gagtgcagag ttataaaaaa ggtatcattg aatgtcgtaa
    18781 gaatattact atcaacgaac cttactttca aggacatttt ccacaacgtc ctatagttcc
    18841 gggtgttttg atgattgaga tggcagctca atctgcagct ttactttata ttttagatgc
    18901 acttgatggt agcctcccct ctcttgatga attaacagca gagaaaatag cagaaaaagt
    18961 aggttattta gccagtgtga agaatttcaa attcaagaaa attgttactc caggagacca
    19021 gctaaacatt acatgtaaat ctcaatcaaa attggggcat cttttagaaa ttcaagtcgt
    19081 tattagagat gagaataaaa aagaggttgc ctcaggaagg atgttggtat cagaaaatgg
    19141 aaattaaact caaaaatatt ggaaaaaaat atggttcttt tgaagcattg agagacatta
    19201 atctcatctt tgaagaggga aaattttatg gccttcttgg acctaatgga gctggtaaaa
    19261 caactttatt taacctttta attcaaaatt ttaagcaaac gtcaggcgat attaagtggg
    19321 aggtggatgg aaaacctcta tctattaaag atttttatcg tcacattggt attgtcttcc
    19381 aaagtaatcg cttagatgat aatttaacgg ttgaggaaaa tctaatttct agaggtgctt
    19441 tgtacggttt atctaaatca caagtacgta atcgccttaa agatcttcaa acgtatctag
    19501 atatcacagc aataaaaaaa caaaaatatg gcagtttatc aggaggtcaa aaacgaaaag
    19561 ttgatattgc acgcgccttg ctgccacaac cttctctatt attattagat gaaccaacga
    19621 ctggtttaga ccctcaatct cgtcgcgatt tatgggatgc tatcgcacaa ctaaatcaac
    19681 aatcgcagat gacagttgta ctaattactc attatcttga agaaatgtct gcttgtgatg
    19741 ttttaaatgt tcttattgaa ggaaacattt attattcggg agatattaaa agctttattg
    19801 agcaacattc cacaacaaac ttgaatgtgg tattgaaacc agaaaagtca ttagaccaac
    19861 tttcgatagc tgattttgtt aataaatgtc aagtcctttc agaaagggag atagtattta
    19921 aggatatttc tgttgaggaa atgatgcagg ttatctcaga caatcaagat aaatctatta
    19981 ttgagacatt taatgttgaa tattcgaact tagaggcggc gtatctcaac ttgcttagag
    20041 aaaaagaaag gaaaaacaat gtttgaacta atttctagaa atcgtaaagt ttacacgcga
    20101 gatcggttag cgtttttcat gtctttttta tcagttatta tcttaatttt ggtctatcag
    20161 attttcttgg gaaaaattca attagatgct ataaaagcag cattgaggag taatacagta
    20221 tccagtgata ctattcacat ggttaattat tggctcattg caggtttgac gactattatt
    20281 tcaatgacta gtacattagg tgcctttgga gttatggtgt ccgacaaaga aaaaaaatta
    20341 aatgaagatt ttaaggtcag tcctatatca aatatcaaaa ttgaattgtc ctatgctata
    20401 tttgccgttt tatttggtat tgtcatgacg atgttctctt gcatctttgc tatcggtatt
    20461 tttaacggct ttaattcctt gctagagtat tcagctatgg attatttaac tattttaggg
    20521 atcgtttcct tagggactgt tttatctgcg gcgatgattt tacctatttt agcctttatt
    20581 catactagtt cagcttttac gactcttagc acaattgtag ggacttttat tggttttatt
    20641 tcaggagtat atctctcaat tggctcggta ggaaaagcat tacaacaggt tatgacatgg
    20701 tttccattga ctcaaatcaa ttctcttttg aaacaggtct taatgaaggg ttctattgcg
    20761 aaggtatttg acaaagccaa cgaagccact gtctctaact ataaagaatc atatggtgtt
    20821 gttttgcgta atgctgatgg agaaaggctg agtaatcact ttatgttgat ttatatcatt
    20881 gccctcattc ttattttatt ggcaattcat tttattatta aaaaggttaa aaaatgaaag
    20941 atgataataa attaaagatt tctgaagctt ccttagaaga ttattctgaa gtggttcatt
    21001 tatttaatag gaatcatgtt tatcaatttc cagacggtag gcctttaact gttgatgacc
    21061 ttgatttaac gttaaaagtt aaagaagtga cacatttatt tttactgaaa aatcatggtg
    21121 ttttgattgg aacatcagct ttctttaaat ttattaccta tggttgttta gattggaata
    21181 gtagctttag tggttttctt ttaattgatt cgaaaagtcg tagtggacag gcaatcactt
    21241 atctatataa aactatctta aagaagataa caaaactgaa gttttctaat atctataccg
    21301 aaatcagtaa ctacaataaa ccctccttag ctttatcaaa attgaatggc ttcaaagagt
    21361 atgataagac atatgaagac atactgcatt gtcgatcatt gcgtagtcac ctccctaaga
    21421 tattgaatac gtttcgtatt tcgaattatt atggtaaaac atacgacata tcaacttttc
    21481 aaattatgga ggaaattgaa aatcccttgg aagaggagac agaaattagg accaaagtct
    21541 ctgatgaaga aatattattt aaagcagaag atagtgcttc acttccttat tatttaaaaa
    21601 tgagcttgtt tcaaatggaa attgctaagt tagataaccg ttatgtttta caagttgact
    21661 ttttatcgga acaagtaaag agggttcggg ttaaaactgg aaaatatcac ttggcaaact
    21721 taacgagggc tcaccctagc ctaactttaa gtcgttttgc caattattat tacattcaag
    21781 caactgtgga aaccctttat ggtaatatcg atgttcaact ggaacgtcga aaaaagcatt
    21841 atagagatgc aactatttgt cttaagagaa cttttcaagg ttatgattta cttatctctc
    21901 caaatggcag ccttattttt gaaaagcaga aaagaaagat acttgaagat agttttttga
    21961 tttttagcca acctttggac aagaaattag ttgtaaaaga agaagaaaat cacattacaa
    22021 tcaaatgttt ctatcaaggt gctttaattg agaaaatcat gacatttaca agtgacgaag
    22081 aaatcacttg tgtttataaa tgtaatcaaa aggctaaaga gatgttccca aaattattga
    22141 aacagacttt caagttacac tgccaagagc agttgattag agatagtgaa ggttatcttg
    22201 tgaatgttcc aggaagttac ccgattgagc atgatgattt tcttcgagca gataagtttg
    22261 aagatagaca attccattat tacctcccga atgaagataa aatgatttcc tattctcctc
    22321 ctggcaaagc cagcaatcaa atgcaattta gacccttatg tcttattgat acagatagcc
    22381 tttcttttcc actgacttat cattttagga tttcacaagc atcatcggag gaggcgttaa
    22441 tcaatctaaa aaaacagcca atatgggaca gcaactacca agcgtcagca actgaccttt
    22501 taaaacatat ctctaaccta acattagaag aagagaagga ttatggcatc aagagaatga
    22561 tagctaacag aaagcattat cctagtcaca aactggtttt agcctataat cagattgttt
    22621 tgcctaagaa tgaaattcca agagacagtg aattatattc tatttctttt gattatcgaa
    22681 ttagaggaaa gtttgtccaa atccgccaag gggaacatgt caaatatgat aataaatcct
    22741 atgttttgga aaatagtcaa caattagtat tgta
//
LOCUS       DATRQA010000015        22756 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.015, whole genome shotgun sequence.
ACCESSION   DATRQA010000015 DATRQA010000000
VERSION     DATRQA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22756
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.015"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..345)
                     /locus_tag="VBD27_000438"
     CDS             complement(<1..345)
                     /locus_tag="VBD27_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO7024473.1"
                     /translation="MMVLERERFMTESDIKVIKRDGRLVSFDKYKIYTALLKASNKVI
                     KMSPLVEAKLEMIADHVIAEIYNRFKDNIKIYEIQNIVEHKLLEANEYAIAQEYINYR
                     TQRDFERSQATDI"
     gene            complement(444..1985)
                     /locus_tag="VBD27_000439"
     CDS             complement(444..1985)
                     /locus_tag="VBD27_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024474.1"
                     /translation="MVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTL
                     LAIANPLLTYFANGLQTQNLYTGLMMTKGQIPYSDVFATGGFLYYVTIALSYLLGSSI
                     WLFIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYPIQWALP
                     FMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQK
                     QSLRGFYQFLCVVFGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLS
                     LAIQIVVLLGSGCLFGLWDFIQNRKKASYQIGLNFIACIFIIYAIMAIFSRDFNLYHF
                     LPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLPLLLVSLL
                     IGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVL
                     PHINTAQKNNEKILKDELLQHGAKYFILNKNEKLPNELKSDIKKHYQEVPLSNITHFV
                     LYRFK"
     gene            complement(2236..2553)
                     /locus_tag="VBD27_000440"
     CDS             complement(2236..2553)
                     /locus_tag="VBD27_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEO7024475.1"
                     /translation="MAHNHNHGHNHEHQHEVITLVDENGNETLFEILLTIDGREEFGK
                     NYVLLVPAGAEEDEQGEIEIQAYSFTENADGTEGDLQPIPEDSDAEWDMIEEVFNSFL
                     DEE"
     gene            complement(2579..2998)
                     /gene="ruvX"
                     /locus_tag="VBD27_000441"
     CDS             complement(2579..2998)
                     /gene="ruvX"
                     /locus_tag="VBD27_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO7024476.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEESGNFGFDR
                     LAELVKEYKVDKFVVGLPKNMNNTSGPRVEASQVYGDKITELFNLPVEYQDERLTTVQ
                     AERMLVEQADISRGKRKKVIDKLAAQLILQNYLDRMF"
     gene            complement(2998..3264)
                     /locus_tag="VBD27_000442"
     CDS             complement(2998..3264)
                     /locus_tag="VBD27_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEO7024477.1"
                     /translation="MGFTDETVRFRLDDSNKVEISETLTAVYRSLEEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDL"
     gene            complement(3466..3864)
                     /gene="spx"
                     /locus_tag="VBD27_000443"
     CDS             complement(3466..3864)
                     /gene="spx"
                     /locus_tag="VBD27_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO7024478.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHQLPYKEQNLGKESLTRDEILE
                     ILTKTESGIESIVSSKNRYAKALNCNIEELSVNEVIDLIQENPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNVENAEARLRAAL"
     gene            complement(4080..5219)
                     /gene="recA"
                     /locus_tag="VBD27_000444"
     CDS             complement(4080..5219)
                     /gene="recA"
                     /locus_tag="VBD27_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO7024479.1"
                     /translation="MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYSSVRLDVRGNTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAF
                     VEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSENAKKYLADNPAIFD
                     EIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE"
     gene            complement(5293..6552)
                     /locus_tag="VBD27_000445"
     CDS             complement(5293..6552)
                     /locus_tag="VBD27_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEO7024480.1"
                     /translation="MRAEIIAVGTEILTGQIVNTNAQFLSEKMAELGIDIYFQTAVGD
                     NEERLLSLLDIASQRSQMVILCGGLGPTEDDLTKQTLAKFLGKSLTVDLLASQKLDRF
                     FASRPQFARTPNNERQAQLVEGSIPLQNLTGLAVGGIVTSKGVQYMVLPGPPSELKPM
                     VMEQVVPILSNNGTKLYSRVLRFFGIGESQLVTILEDIIKNQTDPTIAPYAKIGEVTL
                     RLSTKAENQNEADFKLDSLEKEILALKTLDNRKLKDLLYGYGDNNSMARTVLELLKVQ
                     NKTITAAESLTAGLFQSQLAEFSGASQVFNGGFTTYSMEAKSQLLGIPKKKLQEYGVV
                     SHFTAEAMAQQARQLLKADFGIGLTGVAGPDELEGYPAGTVFIGIATPEGVSSIKVSI
                     GGKSRSDVRHISTLHAFDLVRRALLKI"
     gene            complement(6641..7192)
                     /locus_tag="VBD27_000446"
     CDS             complement(6641..7192)
                     /locus_tag="VBD27_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO7024481.1"
                     /translation="MKRCSWVNLDNPLYVAYHDKEWGRAVHDDHVLFELLCLETYQSG
                     LSWETVLNKRQEFRQVFHHYNIEKVAAMSDADLEIILQNPRVIRHRLKLFSTRQNARS
                     IILIQKEFGSFDRSIWSFVDNKVQVNSVNNYNDVPASTTLSERLSKDLKKRGFKFVGP
                     TCLYSFIQAAGMVNDHENICDFK"
     gene            complement(7215..7805)
                     /gene="ruvA"
                     /locus_tag="VBD27_000447"
     CDS             complement(7215..7805)
                     /gene="ruvA"
                     /locus_tag="VBD27_000447"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO7024482.1"
                     /translation="MYDYIKGKLSKITAKFIVVETAGLGYVIYVANPYSFSGYVNQEV
                     TIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGIGPTTALAIIAVDDNEGLVSAID
                     NSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
                     ALGYKATELKKVKAFFEGTNDTVEQYIKSSLKMLMK"
     gene            complement(7807..9039)
                     /locus_tag="VBD27_000448"
     CDS             complement(7807..9039)
                     /locus_tag="VBD27_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7024483.1"
                     /translation="MKEFFALPKQLQLRELLRFISITVGSAIFPFMAMYYVQYFGNLV
                     TGILIIITQLSGFVATLYGGHLSDAIGRKKVVIVGSLLATIGWAITIAANVPNHITPH
                     LTFVGILIIEIAHQFYSPAYEAMTIDLTNEQNRRFVYTIGYWLVNIAVMLGSGIAGIF
                     YDHHFFELLIVLLIISAICCLVVYFKFDETKPQEGTFKHDKGVLGTFKNYSQVLVDKA
                     FVVYTLGAIGSSVVWLQVDNYFSVNLKQNFEVVSVLGHTITGAKMLSLAVFTNTLLIV
                     LLMTTINKFIENWPLKRQLILGSLICGFGMLFNISLNTFGAILIAMTFFTFGEMIYVP
                     ASQVLRAEMMVEGKIGSYSGFLAIAQPVASVLAGAMVSLSYFTGKIGVQITLTIFMLA
                     GLVLILYATKMKNIEIGK"
     gene            complement(9071..11044)
                     /gene="mutL"
                     /locus_tag="VBD27_000449"
     CDS             complement(9071..11044)
                     /gene="mutL"
                     /locus_tag="VBD27_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO7024484.1"
                     /translation="MSKIIELPDILANQIAAGEVVERPSSVVKELVENAIDAGSSQIT
                     IEVEESGLKKIQITDNGEGMTSEDAVLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     IASISLMTIKTATEQGKQGTLLVAKGGNIEKQEVVSSPRGTKILVENLFFNTPARLKY
                     MKSLQSELAHIIDIVNRLSLAHPEVAFTLINDGKEMTKTSGTGDLRQAIAGIYGLNTA
                     KKMIEISNADLDFEISGYVSLPELTRANRNYITLLINGRYIKNFLLNRSILDGYGSKL
                     MVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKERELMSLISSAISESLKQYDLIPD
                     ALENLAKTSTRSVDKPIQTSFSLKQSGLYYDRTKNDFFIDADTVSEPIANFTNLDKSD
                     GSVDNDVKNSVNQGATQSPNIKYASRDQADSENFIHSQDYLSSKQSLNKLVEKLDSEE
                     SSTFPELEFFGQMHGTYLFAQGNGGLYIIDQHAAQERVKYEYYREKIGEVDNSLQQLL
                     VPFLFEFSSSDFLQLQEKMSLLQDVGIFLEPYGNNTFILREHPIWMKEEEVESGVYEM
                     CDMLLLTNEVSVKKYRAELAIMMSCKRSIKANHTLDDYSARHLLDQLAQCKNPYNCPH
                     GRPVLVNFTKADMEKMFKRIQENHTSLRDLGKY"
     gene            11270..11473
                     /locus_tag="VBD27_000450"
     CDS             11270..11473
                     /locus_tag="VBD27_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO7024485.1"
                     /translation="MTQGTVKWFNSEKGFGFISSETGTDVFAHFSEIKVDGFKTLEEG
                     QKVTFDIQDGQRGPQATNINLVK"
     gene            complement(11530..14106)
                     /gene="mutS"
                     /locus_tag="VBD27_000451"
     CDS             complement(11530..14106)
                     /gene="mutS"
                     /locus_tag="VBD27_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO7024486.1"
                     /translation="MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAA
                     QILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVV
                     KREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
                     SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEI
                     SAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSL
                     YWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVY
                     DIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKIDTMPELE
                     SLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGT
                     LKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEMLEAREQS
                     SNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENHHYVRPKFNDEHQI
                     KIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQ
                     MGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFD
                     ELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLE
                     RDGEVTFLHKIEPGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSAPQ
                     EAVTADLNEELDPGKQQGQLSLFEEHSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKK
                     LL"
     gene            complement(14163..14600)
                     /gene="argR"
                     /locus_tag="VBD27_000452"
     CDS             complement(14163..14600)
                     /gene="argR"
                     /locus_tag="VBD27_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO7024487.1"
                     /translation="MNKIERQKRIKRLIQSGQIGTQEEIKLHLKNEGIDVTQATLSRD
                     LREIGLLKLRSPEGKLYYSLSTATSNRFSPALRSYILKVSRASFMLVLNTNLGEASVL
                     ANFIDEKGLPEILGTMAGADTLLVICQNEDIAKVFEKELSVGL"
     gene            14822..16513
                     /locus_tag="VBD27_000453"
     CDS             14822..16513
                     /locus_tag="VBD27_000453"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO7024488.1"
                     /translation="MDTKHLIASEIQKVVPYMEQSAILSLLETPKNSSMGDLAFPAFS
                     LAKTLRKAPQIIASDIAEQIKSDQFEKVEAVGPYVNFFLDKAAISSQVLKQVLSDGSA
                     YATQNIGEGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFDKIGYHPVKINHLGD
                     WGKQFGMLIVAYKKWGNEEAVRAHPIDELLKLYVRINAEAEIDPSVDEEAREWFRKLE
                     ANDPEATELWQWFRDESLLEFNRLYDKMNVTFDSYNGEAFYNDKMDEVLELLESKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSIYVVGNE
                     QSAHFKQLKAVLKEMDYDWSDDMTHVPFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKDKVAQAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKANFNPSNSDNYSLNDVESWEIIKLIQDFPRIIVRAADNFEPSIIA
                     KFAINLAQCFNKYYAHTRILDEDAEISSRLALCYATATVLKESLRLLGVDAPNEM"
     gene            16601..16909
                     /locus_tag="VBD27_000454"
     CDS             16601..16909
                     /locus_tag="VBD27_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO7024489.1"
                     /translation="MPSEKEILDALSKVYSEEVIQADDYFRQAIFELASQLEKEGMNS
                     LLATKIDSLINQYVLTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(16936..17808)
                     /locus_tag="VBD27_000455"
     CDS             complement(16936..17808)
                     /locus_tag="VBD27_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7024490.1"
                     /translation="MLKLDLKTKIKEAILIAFGVALYTFGFVKFNMANHLAEGGISGV
                     TLIIHALFGVNPALSSLLLNIPLFILGARILGKKSLLLTIYGTVLMSFFMWFWQQIPV
                     TVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLGQTLLFI
                     DAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESAAATI
                     LHEINRGVTFLRGQGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(17916..18860)
                     /locus_tag="VBD27_000456"
     CDS             complement(17916..18860)
                     /locus_tag="VBD27_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7024491.1"
                     /translation="MKTRRTPLEKKVKYIISVWAKKFGLLHTLKSISREKYAEKISAS
                     LLYGILSSVAVNFFFQPGHVYSSGATGLAQVISAVSKHWFSFEIPVALAFYAINIPLL
                     ILSWRKIGHKFTIFTFITVTVSSIFIQLMPQITLTTDPLINAIFGGLIMGAGVGFSFK
                     SRISSGGTDIISLTIRKKTGRDVGSISFIINGIILLFAGLLFGWKYALYSMVTIFVSS
                     RVTDAIFTKQKKMQAMIVTSKPDCVIKRIHRDLHRGVTCINDAEGTYNHEKKAVLITI
                     LTREEFSDFKYLMLKADPKAFVSVAENVHIIGRFVDDD"
     gene            complement(18850..20601)
                     /gene="aspS"
                     /locus_tag="VBD27_000457"
     CDS             complement(18850..20601)
                     /gene="aspS"
                     /locus_tag="VBD27_000457"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO7024492.1"
                     /translation="MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEEVAASVMATAESLRSEFVIEVSGVVTAREQANDNLPTGEVELKVQELSV
                     LNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDNLEF
                     IDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAY
                     DDAMNNYGSDKPDTRFDMLLQDLTEIVKEVDFKVFSEASVVKAIVVKDKADKYSRKNI
                     DKLTEIAKQYGAKGLAWLKYVDNTISGPVAKFLTAIEGRLTEALQLENNDLILFVADS
                     LEVANETLGALRTRIAKELELIDYSKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     AETAHELEGDLAKVRAVAYDIVLNGYELGGGSLRINQKDTQERMFKALGFSAESAQEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SEQQLEELSLTVESYEN"
     gene            complement(20694..21974)
                     /locus_tag="VBD27_000458"
     CDS             complement(20694..21974)
                     /locus_tag="VBD27_000458"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO7024493.1"
                     /translation="MKLQKPKGTQDILPGESAKWQYVENVIRNLFKQYHYDEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFHDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PTKMYYIGSMFRYERPQAGRLREFHQVGVECFGSNNPTTDVETIAMGHHLFEDLGIKN
                     VKLHLNSLGSPESRQAYRQALIDYLTPIREQLSKDSQRRLNENPLRVLDSKEPEDKLA
                     VENAPSILDYLDESSQAHFDAVCHMLDALNIPYIIDTNMVRGLDYYNHTIFEFITEIE
                     DNELTICAGGRYDGLVSYFGGPETPAFGFGLGLERLLLILDKQGISLPIENTIDLYIA
                     VLGSEANLAALDLAQSIRHQGFKVERDYLGRKIKAQFKSADTFNAKVIMTLGSSEVDS
                     KEVGLKNNQTRQEVKVSFENIKTDFSSVLKQLGL"
     gene            22194..22376
                     /gene="rpmF"
                     /locus_tag="VBD27_000459"
     CDS             22194..22376
                     /gene="rpmF"
                     /locus_tag="VBD27_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO7024494.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            22392..22541
                     /gene="rpmG"
                     /locus_tag="VBD27_000460"
     CDS             22392..22541
                     /gene="rpmG"
                     /locus_tag="VBD27_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7024495.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
BASE COUNT         7282 a         4559 c         3543 g         7372 t
ORIGIN      
        1 gatatctgta gcctgtgaac gctcaaaatc gcgttgggta cgataattga tatattcttg
       61 tgcaatagcg tactcattag cttccaataa tttatgttca acgatatttt gtatttcata
      121 tatcttgatg ttatctttaa aacgattgta gatctcagcg atgacatgat ctgcaatcat
      181 ctcaagttta gcctcaacta aaggagacat tttgataact ttattactag cttttaatag
      241 agccgtgtag attttatact tatcaaagct gaccaaacgc ccgtcacgtt taatcacttt
      301 gatatcagat tctgtcataa atcgttctct ttccaatacc atcatgtgaa atatcctcca
      361 gtgacaatta tagcatgcaa agaaaaaaaa tcaatatatt gtgtcaagtt ttttgctcag
      421 acacaatata ttgatttttt aatctatttg aaacgatata gaacaaaatg agtgatattt
      481 gatagaggaa cttcttggta gtgcttctta atatcagatt tcagttcatt tggtaacttc
      541 tcatttttat ttaagataaa gtatttagcc ccatgctgaa gcagctcgtc cttcaaaatt
      601 ttctcattat ttttttgcgc tgtattaata tgtggcaaaa caaattgcga agcagatttt
      661 aaccgagtct ggctataaat gctagcaact ttgtcccaaa cataaatctt accatctcta
      721 tcaatttttg tagtcaaata atgtgaaatg tcacgccttt ctttactaag agtaacattt
      781 tgataagtat tataaacaag taaccctatc aataaggaaa ctagtaagag gggcaaataa
      841 tatgttttct ttacaaaaag gtctacgata agagatttac cagagaaata ctgacgtcta
      901 tgtgaacgcc tgtcaatcac tttttggtaa agaatagtaa ttttattact cgttagcaaa
      961 agaccaaatg gtaaagcagg taaaaagtgg taaaggttaa aatctcttga aaaaatggcc
     1021 attatagcgt aaataataaa aatacaggca ataaagttca agccgatttg ataagatgct
     1081 tttttacgat tttgaataaa atcccatagt ccaaagaggc aacctgaccc tagaaggaca
     1141 acaatttgaa ttgctaaact aagaaggaat gagtggtttg tcctagcgaa atacgtaaag
     1201 gggtaaaaaa tagctttgtc aatataagaa gagataatct gtagattaaa cataaagtaa
     1261 ccaacagtat aagcaattaa aatcatacca aaaacaacgc ataaaaactg atagaaccct
     1321 cgtagtgact gcttttgctt aatattggtt gctgttaaga gaacaaaaga gcaaagccaa
     1381 aaaatcagtg tttggggatc tataaatagt gagaatgctg ctaaaatacc ataaaatata
     1441 aatgcttcat caacgatatt atcgacacaa aatttaatta aaaaccatag cgaaatgagc
     1501 ataaaaggta atgcccactg tattgggtac ataccaccga atcctaagac aatattcatt
     1561 atatagaaaa tcaacgtcat gcctatcgag acaatttcac tttgtgccac ataataaact
     1621 aatttataaa aataaattcc agatacatag taagcaataa actgtacaat aaataaccag
     1681 atactagacc ctaaaaggta acttagagca atcgttacat agtataaaaa tcctcctgta
     1741 gcaaaaacat cactataagg gatctgtcct ttggtcatca tcaaacctgt atagagattc
     1801 tgagtctgca aaccatttgc aaaataagtc aataaaggat ttgcaattgc taagagagtt
     1861 gacaaaacac tatatagtaa aattgaaaat cgactagatt tcttcattct gatagccgtt
     1921 cgtttactca ttgatgggct atttttatag ctcactccaa ttggtttttc taggttttct
     1981 accactattt ttctcctcat atttacatac taccagtata tcagaaaacc ttatttagtc
     2041 aatttagcca acacttgacc gtaacgaata atatccctaa agtagtggta tcttctagac
     2101 atatattgtt tgagaaattc ttgttcttct aatgttagct gagtatctga cattaacatc
     2161 acctcctttt actacatcat cactattatt attcgaaaaa aaaaacgact gaacaattgc
     2221 tcaatcgctt caatattatt cttcatctag aaagctgttg aaaacttcct caatcatgtc
     2281 ccattctgca tctgaatctt ctggtatagg ctgaaggtct ccttcagttc catcagcatt
     2341 ttctgtaaat gagtaagctt gaatctcaat ttcaccttgt tcgtcttctt ctgcacctgc
     2401 aggaacgagg agaacatagt ttttgccaaa ctcttcacgt ccatcaatag ttagtaggat
     2461 ttcaaaaagt gtttcgttcc cattctcatc tacaagagtg attacttcat gttgatgttc
     2521 atggttatga ccgtggttat gattatgtgc cattgtttct cctttttatg ccgaatgctt
     2581 aaaacatgcg gtctaaataa ttttgtaaga ttagttgagc tgctaattta tcgataactt
     2641 tcttacgctt accacgacta atatcagctt gctcaactag catacgctct gcttgaacag
     2701 tcgttagacg ttcatcttga tattcaactg gaagattgaa taattcagtg attttatcgc
     2761 catatacctg actagcctct acacgaggac cacttgtatt attcatattt ttaggtagac
     2821 caactacaaa tttatcaact ttgtactctt ttactaactc tgctaagcgg tcaaaaccaa
     2881 aatttccaga ttcttcatcg atcttaatga tttcaagacc ttgtgcagtg aaaccgagtg
     2941 gatcactaat tgcaacgcca acagttttcg agccgacgtc aagtcccatt attctcatta
     3001 caaatctatt ccatttcctt gtaaataata acgaacgagc tcttctacaa tctcatcacg
     3061 ttcatattta cgaatttgat tgcgagcatc attgtaacga ggtacataag cagggtcccc
     3121 actcaatacg taaccaacaa tttgattgat gggattgtaa cctttttctt ctaatgaacg
     3181 ataaacagct gttaatgttt cactaatttc tactttgtta ctatcatcta atctaaaacg
     3241 aacagtttca tctgtaaatc ccatacctac accttctttc ctttaattct ttcacttatt
     3301 ataacttaaa taaagtattt gtacaagaaa ttgataaaca ctatattaga aaatcaaaaa
     3361 gggagcacaa gaaaccaaga ggtgattctt tgatttcttt actccctacc ttataggtga
     3421 tttttacagg ttatcatgtg actctcactt atgcttatct aaagctcaaa gtgctgcacg
     3481 aagacgagct tctgcatttt caacgttacg aatagagcga ggtagaaaag cacgaatatc
     3541 atcttcctta taacctactt gaagacgttt gtcatcaatt agaattggac tctttaaaat
     3601 acgtggattt tcttgaataa gatcaataac ttcatttaca ctcaactcct caatattaca
     3661 attgagcgcc ttagcataac gatttttaga agaaacgata ctttcaatac cactttcagt
     3721 ttttgttaat atttctaaaa tttcatcacg tgttagtgat tctttaccta ggttttgttc
     3781 tttatatgga agttgatgag catttagcca agtttttgct tttttacaac ttgtgcaact
     3841 tgaaatagta taaatcttaa tcatgtgcct tctcactttc tatcccatga tagttatagt
     3901 ttaacatagt cacaaaaatc aaacatggga cttaagacct attttttatc aatttactaa
     3961 taaaaataaa tagattttag ggatttttaa gcttattttt agtataaaaa taagttccaa
     4021 ccactgtcag aacttatttt agttacattt attcaagttc aagatgtata gttgctattt
     4081 tattcctcaa tttcaatagc attgtccaaa tctaaaacaa gatcatcagc ttcatttttt
     4141 tcttcaacga gctcagactg aactggcgaa tcatcttcag tcattccaaa gtgcacgcgc
     4201 actttatggt caatttcatc aaaaattgct ggattatctg ctaaatattt tttggcattt
     4261 tcagaacctt gtccaatttt ctcaccatta tatgagtacc atgcacctgc tttttggata
     4321 atatccaaat cactagcaat tttaattaac tcacccgtgc gagaaatacc ttcaccatac
     4381 ataatttcga caaaagcttc acggaatggt ggagcaactt tatttttaac aactttgatt
     4441 tttgtctctt taccaacatt atgatcttta tgctcaccag tccccttgat ttgagtatta
     4501 ccacgaacat ctagacgaac cgatgaataa aatttcaaag cacgaccacc aggtgttgtt
     4561 tcagggttac caaacataac gcctactttt tcccttagct ggttgataaa aattgcgatt
     4621 gtctttgtct tgttaatgga tgctgacaat ttacgcattg cttgactcat catacgagct
     4681 tgcaaaccaa cgtggctgtc accaatatca ccatcaatct cagcacgagg aacaagggct
     4741 gcaactgaat cgacaacaac taaatcaact gcgcctgaat caatcaattt acctgcaatt
     4801 tcaagacctt gctcacctga gtcaggttga gacaagagta actcatcaat attaactcca
     4861 agagccgcag cataggctgg gtcaagagcg tgctcagcat caataaaggc agcaatccca
     4921 ccttcctttt gggcttgagc tacggcatga agggcaaccg ttgttttacc agatgactct
     4981 ggtccataaa tttcgacgat acgtccttta ggatatccac ctgctcctaa agcgatatct
     5041 aaagcaagag aacctgaact cattacttga accttttgtt cagctcgctc acctaagcgc
     5101 atcactgcgc ccttaccaaa atctttttca atatttttca aagcatcatc taaggctttt
     5161 cgacgttcat caccgaattt cttagtaatt tcttctgctt ttttcgtttt tttagccaat
     5221 gtcttctcct tatatttcta tgaatctagg ttgattcaaa tttagaaatc gttctactaa
     5281 ttataacaaa tattatatct ttaataaagc cctacgcacc aggtcaaaag catgtaaagt
     5341 actgatatga cggacatctg agcggctctt ccctccaatc gatactttaa tagaagaaac
     5401 gccttcaggg gttgcaatcc caataaaaac tgtacctgca gggtatcctt ccaactcatc
     5461 aggaccagca actcccgtta aaccgatacc aaaatctgct tttaaaagct ggcgagcttg
     5521 ctgagccatt gcctcagctg taaaatgact gacaactcca tattcttgca attttttctt
     5581 agggatgccg agaagttgtg attttgcttc catactatag gtcgtaaagc caccattaaa
     5641 aacttgcgaa gcaccagaaa attctgctaa ctgagattga aataaccctg ctgttaagct
     5701 ctctgcggca gtaatcgttt tattttgtac tttcaataat tctaaaacag tacgtgccat
     5761 actattatta tcaccatagc catataataa atcttttaat ttacggttat ctaatgtctt
     5821 aagagcaagt atttcttttt ctaagctatc taatttaaag tcggcttcat tctgattttc
     5881 agctttcgtt gataacctta aagttacctc accaatcttt gcatatggtg ctatagttgg
     5941 atctgtctga ttcttaatta tgtcttctaa gattgtgact aactgactct caccgatacc
     6001 aaagaatctc aatacacgtg aatataattt tgtgccatta tttgacaaaa taggtaccac
     6061 ctgctccata accattggct ttaattcact aggcggacct ggtaaaacca tatattggac
     6121 tcccttagat gtaacgatac caccaacagc tagaccagta aggttttgta gaggtattga
     6181 gccttcaacc aattgagctt gtcgttcatt atttggcgtt ctagcaaact gaggacgact
     6241 tgcaaaaaaa cgatccaatt tttgactagc taataaatcc actgttaatg atttacctaa
     6301 aaatttagcc aaagtttgtt tcgttaaatc atcctctgta ggtccaagtc caccacatag
     6361 gataaccatt tgactgcgct gactagctat atctaaaagc gataataaac gttcctcatt
     6421 atctcctaca gctgtctgga aataaatatc aattcccaat tctgccattt tctccgacaa
     6481 aaattgtgcg tttgtattaa cgatctgtcc tgttaaaatt tctgttccta cagcgataat
     6541 ttcagccctc acaacttcac cctcttatct attcgaaata atgactacat tttagcaaaa
     6601 aaagtagcag aagtgctact taaaatatat cacattgata ttacttgaaa tcacatatat
     6661 tttcatggtc attaaccata cccgcggcct gaataaaaga atagaggcaa gttggaccaa
     6721 caaatttaaa accgcgtttt tttaaatcct tagataagcg ttcggataag gtagtacttg
     6781 caggcacatc gttgtagttg ttaactgagt taacctgtac tttattatct acaaaagacc
     6841 aaatggagcg atcaaaactt ccgaattctt tctgaattaa tataattgac ctagcatttt
     6901 gtcgtgttga gaaaagtttt agacgatgac gaataaccct aggattttgt aagattatct
     6961 ctaaatccgc atctgacatt gcagctacct tttcaatatt atagtgatga aaaacttgtc
     7021 tgaactcctg gcgtttattt aatactgttt cccaagataa acccgattga taagtttcta
     7081 aacatagtaa ctcaaagagt acatgatcgt catgaacagc tctcccccac tctttatcat
     7141 ggtaagctac atataatgga ttatctaaat tcacccaaga acatcgtttc atgtgaaacc
     7201 tcattacttc taaattattt catcaacatc ttcaaactag atttgatgta ttgctccaca
     7261 gtatcgttcg taccttcaaa aaaggctttt acttttttca attcagtcgc cttatacccc
     7321 aacgctaaca aagcttccat agcttcttca agattgctat tctgctcaga gctaacctta
     7381 cgactagtcg cactctcacc agaagcttct acaaacttcc ctgacaaatc aaggatcatc
     7441 tgttgtgctg ttttttttcc tatctttgga aatttagtca aatacttaat atcactatta
     7501 tcaatggcac ttacgagacc ttcattgtca tcgactgcaa taattgctaa agcggtcgtt
     7561 ggaccaattc ctgatactga aattaaatta agaaaaattt ctttttcatt ttccgtatga
     7621 aaaccaaata aaagatgtgc atcatcgcga atgacttgat gtaagtagat ggtaacttct
     7681 tgattaacgt aacctgaaaa agagtaggga tttgccacat atatcacgta tcccaatcca
     7741 gctgtttcca caacaataaa tttagccgtt attttactca attttccttt aatataatcg
     7801 tacattctat tttcctatct caatattttt cattttggta gcgtataaga ttaaaaccag
     7861 acctgctaac ataaaaatgg tcaatgttat ttgaacacct atttttcctg taaaatagct
     7921 gagcgaaacc atggcacctg ctaatacact tgcaacaggt tgtgcgatag ctaaaaatcc
     7981 agaatatgat ccaatttttc cctccaccat catctccgcc cttagaacct gactagctgg
     8041 aacataaatc atttcgccaa aggtgaaaaa cgtcatggca atcaaaatag ctccaaaagt
     8101 gtttagagaa atattaaaaa gcattccaaa accacaaatc aagcttccta atattagttg
     8161 acgtttcaaa ggccaattct ctatgaattt atttatagtt gtcattagta agacaattaa
     8221 tagagtgttc gtaaagactg ctaaactaag cattttagca cctgtgattg tatgtcctaa
     8281 cacagacact acttcaaaat tttgcttaag attgaccgag aaataattat caacctggag
     8341 ccagacaaca ctggaaccaa ttgctcctaa agtataaaca acaaacgctt tatccactaa
     8401 aacctgacta taatttttaa aagttcctaa aactccctta tcatgcttga aggttccctc
     8461 ctgtggtttt gtctcatcaa acttgaagta gaccaccaaa caacaaatcg ctgaaataat
     8521 aagaagaact attaataatt caaaaaaatg gtgatcatag aaaatcccag caatcccaga
     8581 acctagcatt acagcgatgt taaccaacca ataacctata gtgtaaacaa agcgacgatt
     8641 ctgttcgttc gtcaaatcaa tggtcatagc ttcataggca ggggaataaa attggtgagc
     8701 tatttcaatg ataaggatac ctacaaaggt caagtgtggc gtaatatgat ttggaacatt
     8761 tgcagctata gttatagccc aaccaatggt tgccagcagt gaacctacaa tcacaacttt
     8821 tttcctccct atagcatctg acaaatggcc tccatataag gtcgctacaa accctgatag
     8881 ttgagttatg atgatcaaaa ttcctgtaac tagatttcca aaatattgaa cataatacat
     8941 tgccataaaa ggaaaaatag ctgaaccaac cgtaatggaa ataaaacgca ataattctct
     9001 taactgtagc tgttttggca aagcaaaaaa ctctttcact ttttctccct tcttccctaa
     9061 caagtaatta tcaatatttt cctaaatccc tgaggcttgt atgattttct tggattcgtt
     9121 taaacatttt ctccatatca gcttttgtaa aattaactaa aacaggtctt ccatgagggc
     9181 aattataagg atttttacat tgcgctaact gatctaataa atgacgtgct gagtaatcgt
     9241 ctagcgtatg gtttgcttta atagatcgct tgcaagacat cataatagcc aactctgcgc
     9301 gatatttttt cactgatact tcattagtga gcaacaacat atcacacatt tcataaacgc
     9361 cagactctac ctcttcttct ttcatccaaa ttggatgttc ccttaaaatg aaagtattat
     9421 taccatacgg ttccaagaaa ataccaacat cttgtaataa tgacattttt tcttgtaatt
     9481 gaagaaaatc tgaacttgaa aattcaaata gaaatggtac taacaattgt tgaagactat
     9541 tatcaacctc acctatcttt tcacggtaat actcgtattt tacacgctct tgagctgcat
     9601 gttgatcaat aatatacaac ccaccattcc cttgagcaaa taagtaggtc ccatgcattt
     9661 gaccaaaaaa ttcgagctct ggaaaagtcg aactttcttc cgaatctaat ttttcaacaa
     9721 gtttgttaag tgactgttta ctactaaggt agtcctgaga atgaatgaag ttctcactat
     9781 cagcttgatc tctactagca tactttatat taggagactg cgttgctcct tgattaacag
     9841 agttttttac atcattgtca actgaaccgt cacttttgtc aaggtttgta aagttagcaa
     9901 taggttcaga tacagtatct gcatctataa aaaagtcatt tttagtccta tcgtaataca
     9961 aaccagactg ttttagtgag aagcttgttt gtataggctt atctacactt cgggtactag
    10021 ttttcgctaa attctctaaa gcatccggaa tcaaatcata ttgtttcaga ctctcagaaa
    10081 ttgctgagct tattaaactc attaattctc gttccttaga aattctcacc tcttgcttag
    10141 tcggatgaac gttgacatct gctagataag gatctatctg aatatcaatg acagcaattg
    10201 gaaatcttcc aaccatcaac tttgaaccat aaccatctag aattgagcgg tttaaaagaa
    10261 aatttttaat gtaacgaccg ttaattaata gggtaatata gtttcggtta gctcgtgtca
    10321 attcaggcaa acttacataa cccgaaattt caaaatctaa atctgcattt gaaatctcaa
    10381 tcattttttt tgcagtattt aaaccataaa tacctgcaat cgcctgtctc aaatcgccag
    10441 tcccactagt tttagtcatt tctttaccat catttatcag tgtaaaagca acttccggat
    10501 gcgctagact gagtctatta acaatatcaa taatatgagc tagttcagac tgtaaactct
    10561 tcatgtattt caatctggca ggcgtattaa agaataaatt ttcaactaaa atttttgttc
    10621 cacgaggact tgaaacaact tcctgttttt caatgtttcc ccctttagca acaagtaacg
    10681 tcccttgttt cccttgctct gtagctgttt ttattgtcat taaactaatc gaagcgattg
    10741 atggtagagc ttcaccacga aaacctaagg tccgaatacg aaaaaggtca ctctggcttt
    10801 tgattttgga tgtagcgtga cgtcgtaaac tgagaacggc atcctcacta gtcatccctt
    10861 caccgttatc cgttatttga atctttttaa gaccagattc ttcaacctct atggtaattt
    10921 ggctacttcc tgcatctatg gcattttcaa ctaattcttt aacgacgcta ctaggccttt
    10981 caacaacctc accagcagca atctggttag ctaatatatc cggtaattca ataattttag
    11041 acaagttcat ccctcctttt tttatgattt tttgtaactt tttttgtgat atcttagcca
    11101 ttataccata ttcaaaatat taatttgaca ataacacaat ttttagttga catcactcct
    11161 attacctgtt agacttaggg agcgtaataa gttttgaagt tgtatttttg tttaacattt
    11221 ttaccttttt tattttttac agtaacattt ttagaaaggt aataaacaaa tgacacaagg
    11281 tacagtgaaa tggtttaact cagaaaaagg ttttggcttt atttctagcg aaactggaac
    11341 agacgtcttc gctcattttt cagagattaa agttgatggc ttcaaaactc ttgaagaagg
    11401 acaaaaagta acatttgata ttcaagatgg tcaacgtgga ccacaagcaa caaatatcaa
    11461 tcttgttaaa taattaaaaa agagcctaaa tgaattattt agtctctttt ttgttgccta
    11521 aaggctattt tataataatt tctttaaatc aaatatagca ttcatagctt gcattggagt
    11581 tagattcatt atatctatcg cttctaactc ctcaataacc ctacctgcat ttgaatgttc
    11641 ttcaaaaagc gataattgtc cttgttgctt cccaggatct agttcctcat ttaagtcagc
    11701 agtaacagct tcttgggggg ctgatacgat caactgtact gcatcagctt ccaactgtga
    11761 taaaatatca gttgccctat ccaataagtc aattggtaaa ccagctattt ttgcgacgtg
    11821 tatcccataa gacttgtccg caggtccagg ttcaatttta tgtaagaagg taacttctcc
    11881 atctctctct aaagtagcca cgtgtacatt gacaagcctt gtcaattgtt cagataaatc
    11941 tgtcaactca tggtaatgag ttgcaaacat tgtttttgcc ctaacacggt catgaatata
    12001 ttctataatc gattgagcta atgccatacc atcataagtg gctgtccctc gacctaattc
    12061 atcaaaaaga atcaaagatt tatcactggc tcgttttaca gcttgattcg cttccatcat
    12121 ttctaccata aaggttgatt gcccagaaat taagtcatca gcagcaccaa tcctagtaaa
    12181 tattgcatca aatacaggca aatcaacttc gtctgccgat acaaaacctc ccatttgtgc
    12241 cataataact gtcagagcta actggcgcat ataggtcgac ttaccactca tatttggtcc
    12301 tgtaatcaac tggatatctg tctgactatc aaaatagata ctattgggaa tatattcttg
    12361 cactcccatc actttttcaa cggttgcatg acgcccattc ttaatcttaa tctgatgttc
    12421 atcattaaat ttgggacgaa catagtgatg attttctgca actactgcca aactctgtag
    12481 aacatcaacg gttgcaatag tctttgctaa ctcttgaaga cgtttaatat aagattctac
    12541 ttgagcacga acacgcataa aaatatcata ttctaaattt gaagattgct cgcgagcttc
    12601 gagcatttca ccttcaattt tggctagttc tgctgttcca tagcgttcag aattttttaa
    12661 tgtcgcttta cggaaaaaat gctccggtac tagtgataaa ttggaattgg tcacatggaa
    12721 gtaatagccg tcttttttat tataatcaat tttaagggta ccgataccac ttgctgctct
    12781 ttccttagct tcaatatcag caatccaacc tgtaccttca cgcatcactg ttcgataatt
    12841 atccaattgc ttatcaaatc cagacttgat aatgtttccc tcagtgatag tagcctgtgc
    12901 ttctggggct atcgccgtat taattaaact ttctaattca ggcatagtgt caattttgtt
    12961 gacaatgatg tcaagctcag gttgattgaa ggactgtaaa atcattttaa tccgaggaat
    13021 ttgtgataag gtctgtccga gttgcaatag atctttaggg ttggcttttc cgaaagatac
    13081 tcgacttgct aggcgttcaa tatcatatac accctttaaa ctttctgtga gatcgtttct
    13141 ctcaaaaaaa taatcaagaa acacttgaat aatatcttga cgttccttga ttcgattcat
    13201 acttaccaaa ggcctgtcaa tccaagttct cagcattcga gttcccatcg ctgttttagt
    13261 ctcatctaac aaccagtaaa gacttccgtg cttcttgctt gttctagcat tctccagtaa
    13321 atctagacta tttttcgttg catatgacat ttgtaaatag tcctttattt cataatgaac
    13381 tactttctgt aaatgactaa gttcacgctt ttgtgttcta tgcacatatt gtaaaagttt
    13441 ttccgcagca gatatttcca aatcagttaa ctgctcatca atcaaatgaa tgtcattaag
    13501 tcgttcgtct tcgtatgata aaagcaagtt catctgcttc gtcagtaggt gatttttttc
    13561 gtccgtcaat tgatatccta ctacaatctc acgagctttt aaatttagta tttcactacg
    13621 gacggactca aaatctgtta aaagggttgc ctgaaactct ccagttgaga catccatata
    13681 tgctagacca aatgtttgtt gatcttgcga atcaatcgct actaagaaat tattagcact
    13741 atccggcttc gttgactcca caaccgttcc tggggtaaca acttgcacta cctcacgctt
    13801 gaccactccc acagcttttt taggatcttc catctgctca gcaatggcta ctttgtaacc
    13861 taattcaact aaaacgtcaa tatactgttg agctgagtga tagggaactc ctgccattgg
    13921 gattggcttt tctgcgttct tatttcgact agtcaagcta atttccagga tttgtgctgc
    13981 ttttaccgca tcatcataaa ataattcata aaaatcaccc attctaaaaa gcaaaaaagc
    14041 atctggataa ttctctttta tatccagata ctgttgcatt cccggtgata tcgttggctt
    14101 ggccatttct cactcctaac taacggggct ggctgccatg ctcagctcct caatcattat
    14161 atttataaac ctactgatag ttctttttca aaaactttgg caatatcttc attttgacaa
    14221 atcaccaaca aggtgtctgc tcctgccatt gttcctaaaa tctctggcag ccctttctca
    14281 tcaataaagt ttgctaaaac gctagcctcc cctaaattag tatttaaaac taacataaaa
    14341 cttgctcgcg atactttcaa aatataggag cgtaaggctg ggctaaatcg atttgaagta
    14401 gctgttgata aactataata tagcttacct tctgggctac gcaattttaa aagaccaatc
    14461 tcacgcaagt ctcttgatag agtcgcttgc gtgacatcga ttccttcatt ttttaaatgt
    14521 aatttaattt cttcttgtgt gccaatctgc cctgattgga ttaaacgttt aatacgtttt
    14581 tggcgttcta ttttattcat gttattcaat cctttttagt tagaattatg cttaccaaat
    14641 tatatcaaaa aaaattgtct ttttcactta gtagtgatat cgtaatcatt ctgtaaaaaa
    14701 gctataaagt aggctagttc tttacagaat gcattatgaa tttaatcaac aagttctcgt
    14761 ctatttttca taattatgtt aaaatagggt gactatatta ctaattaaaa taggagatac
    14821 catggataca aaacatctaa ttgcgagtga aattcaaaaa gttgttccat atatggaaca
    14881 atcggccatt ctttctttat tagaaacccc aaaaaattcg agcatgggag atttagcttt
    14941 cccagcattc tctttggcta aaactctacg caaagcacct caaataattg ctagtgacat
    15001 tgctgaacaa attaaaagcg accaatttga aaaggtggaa gctgttggac cttacgttaa
    15061 ctttttcctt gataaagccg caatctcaag tcaagtttta aaacaagtct tatctgatgg
    15121 ttctgcctat gctactcaaa atattggtga aggacgaaat gttgccattg atatgtctag
    15181 tccaaatatt gctaagccat tctcaattgg acaccttcgt tcaacagtta ttggtgatag
    15241 tttagctaat atttttgata aaattggcta tcatcctgtt aaaattaatc accttggcga
    15301 ctggggtaaa caatttggaa tgttaatcgt tgcctataaa aaatggggca atgaagaggc
    15361 tgtccgtgct catcccattg atgaactttt aaaactttat gtccgtatta atgctgaagc
    15421 cgagatagac cctagcgttg atgaagaggc acgtgagtgg tttcgtaaac ttgaggctaa
    15481 cgatcctgaa gcaactgaat tatggcaatg gttccgtgac gagtcattat tagaattcaa
    15541 ccgtctttac gataaaatga atgttacatt tgatagctat aacggcgagg cattctacaa
    15601 cgacaaaatg gatgaagtgc ttgaactgct agaatctaaa aacctcctag tcgaatctaa
    15661 aggtgcacaa gttgttaacc tagaaaaata cggtattgaa catcctgccc tcattaaaaa
    15721 atccgatgga gcaactctct acattacacg tgacttagct gctgcacttt accgtaaacg
    15781 tacctacgat tttgcaaaat caatctacgt tgtaggaaac gaacagtctg cacactttaa
    15841 acaactaaaa gctgttctta aagaaatgga ctacgattgg tccgatgata tgactcatgt
    15901 tccttttggc cttgtaacaa aaggaggagc aaaattatca actcgtaaag gaaatgtaat
    15961 tctccttgaa cctactgttg cagaagctat taatcgtgcg gcatctcaga ttgaagctaa
    16021 aaaccctaac ctagcagata aagataaagt tgctcaagca gttggtgtag gtgctattaa
    16081 attttacgat ttgaaaactg atcgtacaaa tggttatgac tttgacttag aagcaatggt
    16141 atcatttgaa ggtgaaactg ggccatatgt acaatatgca catgctcgta ttcaatcaat
    16201 cttacgtaaa gctaacttta acccatctaa tagcgataat tacagtctca acgatgttga
    16261 aagctgggaa atcatcaaac tcatccaaga ttttcctcgt attattgtgc gtgcagcaga
    16321 taattttgag ccctctatta ttgcaaaatt cgctatcaat ttagctcagt gcttcaataa
    16381 atattacgcc cacacacgta ttttagatga agatgctgaa atcagtagtc gtctagcact
    16441 ttgctatgca actgctacag tattaaaaga atctcttcgc ctcctaggag ttgatgctcc
    16501 gaatgaaatg taagaaatga cattactaag actgtcaaat ttatgacagt cttaattttt
    16561 tatattataa taatagcaca ataagttaca ggagaatgcc atgccatcag aaaaagaaat
    16621 attagatgct cttagtaaag tttatagcga agaagtaata caagcagatg attattttcg
    16681 tcaagctatt ttcgagctcg ctagtcaact tgaaaaagag gggatgaact cactcctagc
    16741 tacaaaaatt gactcactta tcaatcaata cgtcttaaca catcaatttg atgctcctaa
    16801 aagcatattt gatcttagtc gcttggtaaa aacaaaagct agccattaca agggaactgc
    16861 tatctcagct attatgcttg gtagtttctt aagtggagga ccaaaataaa aatacaggcc
    16921 agacagcctg tatttttatt tatcgtaatt tcttcgtcga attttaaaat ctgagctaat
    16981 aacttcatct acatttataa cagaaataaa agcatctggg tctaaatcag ccatgatttc
    17041 tttcacatca cgcacttcac ttgggttaag agctacgtac aagacatcat gctctctacc
    17101 tgagtaggca ccttggcccc gtaaaaaagt tacaccccta ttaatttcat gcaaaatggt
    17161 tgctgctgca gattctgatt ctttggtaat aatgatcatt ccacgaacag tgtagccccc
    17221 attttcaaca tttgttaaca cttgactaaa tacaaagcta gctactaacg tatatagcat
    17281 ctgttgtaaa ttaatataaa caagtgaaga cgttagaact atagcatcaa taaacaataa
    17341 ggtttgccct aattttattc cagatttttc ctctacaata cgaccaataa tatctgcacc
    17401 acctgtagtt gcaccatatc taaatactaa accactacca gttcctgcta aaattccagc
    17461 agctactgca actaacatca tatcgttttt caaagggact gttacaggga tttgttgcca
    17521 aaaccacatg aaaaaagaca ttaacactgt accatatata gttaataata aggatttttt
    17581 ccctaaaatc ctagccccta atataaataa cgggatatta aggagaagtg atgaaagtgc
    17641 tggattaact ccgaaaagtg catggataat caaagtgaca ccggaaatac ctccttcagc
    17701 caaatgatta gccatattaa atttgacaaa gccgaaagta tagagagcca caccaaaggc
    17761 aattaaaata gcttctttta tctttgtttt taaatccagt ttcaacatag gcttaatcct
    17821 tccttattca aaaaagctaa agttgtttaa acaacttagc ttaataacat cataatgttt
    17881 ccttagtggc ggttgaccaa aagtcatata tacagttaat catcatcaac gaatcgtcca
    17941 ataatatgaa cattttctgc aacggaaaca aaggcttttg gatctgcttt tagcattaaa
    18001 tatttaaaat cactaaattc ttcacgtgtt aatatagtta ttaaaactgc ttttttctca
    18061 tgattataag taccttcagc atcattgata caggttacac cacgatgtaa atcacgatgg
    18121 atacgtttaa tgacgcaatc aggcttactg gtaacaatca tcgcttgcat ctttttttgc
    18181 tttgtaaaaa tggcatctgt aactctactt gatacaaaaa ttgtcaccat cgagtacaaa
    18241 gcgtacttcc aaccaaataa aagacctgca aataataaaa taataccatt aataataaaa
    18301 gaaatactac ctacatctct gcctgttttc tttctaattg tcaaactgat aatatctgtt
    18361 cccccactag aaattctaga cttaaagcta aaaccaacac cagctcccat aattaagcca
    18421 ccaaagatag cattgattaa gggatcagtt gtaagggtga tttgaggcat tagttgaatg
    18481 aagatagagc ttacagttac tgtgataaac gtaaaaatag taaatttatg tccaatctta
    18541 cgccaagata atatcagcaa agggatatta atggcataaa aagctaaagc aactggtatt
    18601 tcaaaagaaa accagtgttt actgacagct gaaataacct gagctaaacc agttgctcca
    18661 cttgaataaa catgtccagg ttgaaaaaag aaattaacag caacactgga tagaatacca
    18721 tacagtaatg aagccgaaat tttctctgca tatttttctc gtgagatact ttttaaagtg
    18781 tgtaagagac caaatttttt agcccataca ctgataatat attttacctt tttttctaaa
    18841 ggggttcgtc tagttttcat aactttctac agtaagtgat aattcttcta attgttgttc
    18901 tgaaacgaga ctaggagctt gagtcattgg atcactagcc ttattgtttt tagggaatgc
    18961 aataacttct cgaatattgt ccttgcctgc taacaacata acaaagcggt caaggcctat
    19021 tgccaatcca ccatgtggtg ggaagccgta atccatagct tcaagtaaga aaccaaactg
    19081 ttcctgagct gattcagcgc taaatcctaa agccttgaac atacgctctt gtgtatcttt
    19141 ttggttaata cgtaggctac caccgcctaa ctcatatcca ttcaaaacaa tatcataggc
    19201 aactgcacgt actttagcca aatccccttc caactcatgc gctgtctccg ctgttggcaa
    19261 ggtgaatgga tgatgagccg acatatatcg tccctcttct tctgaccact caaacattgg
    19321 ccaatcaaca acccatagga aattaaattt agaataatca atgagttcaa gttcttttgc
    19381 aatgcgtgta cgaagcgctc ctaaagtctc gttagctact tcaagactat ctgctacaaa
    19441 tagaattaag tcattattct ctagctgcaa agcttctgtc aacctacctt caatagctgt
    19501 caagaactta gcaaccggac ctgaaatagt attatctaca tattttagcc atgctaaacc
    19561 tttggcaccg tattgttttg caatttctgt taatttatca atattttttc gagagtactt
    19621 gtccgccttg tctttgacaa caatcgcttt aactactgaa gcttctgaga acaccttgaa
    19681 gtcaacttct tttacaattt ctgtcaagtc ttgtaaaagc atatcaaacc ttgtatcagg
    19741 cttatcagac ccgtagttat tcattgcatc atcatatgcc atccttggga atggtaaaga
    19801 aacctctaaa cctttagtgt ctttcattac tttagcaatc ataccctcaa caatgtcttg
    19861 aatttcttga tcactaagga atgatgtctc taagtcaact tgtgtaaact caggttgacg
    19921 atctccacgt aaatcctcat cacgaaaaca tttgacaatt tgataataac gatcaaatcc
    19981 tgcattcatc aatagctgtt ttgtaatttg agggctttgt ggaagggcat aaaaatgtcc
    20041 ttggttgaca cgtgatggaa ccaaatagtc tcgtgccccc tctggagttg attttgttag
    20101 catcggagtt tcgacatcaa taaactctaa gttgtcaagg taattacgga ttgagtgtgt
    20161 tactttcgca cgtaatttga aattttcaag catttccgga cgacgaagat ccaagtaacg
    20221 gtaacgcatg cgtgtatcat catttgcctc aataccatct ttaatttcaa atggtgttgt
    20281 tttcgaggta ttaagaaccg ataattcttg cacttttaat tcaacttctc ctgttggtaa
    20341 attatcatta gcttgttctc gtgctgtcac tacaccgcta acctctataa caaattcact
    20401 acggagactt tctgcggttg ccataactga ggctgctact tcttcaggat taataactaa
    20461 ttgcatgatt ccctcgcgat cgcgaagatc aataaaaata aggcctccaa ggtcacgacg
    20521 acgaccaacc catcctttta aagtaatcga tgttcctatg tgctcactac gaacacgccc
    20581 agcatacata ctacgtttca tctattttct ccatttaaaa tattcattcc aacctattat
    20641 aacaaaaagc acctaaaaac cccacccatt aaggagagtt tttaggaact gttttacaat
    20701 cctaattgtt taagcacaga tgaaaaatct gttttaatgt tttcaaaact aactttaact
    20761 tcttgacgtg tttgattatt ttttaaaccg acctctttcg aatctacttc actgctacct
    20821 aatgtcataa tcactttagc attaaaagta tctgcagact taaattgtgc tttgatttta
    20881 cgacccaaat aatcacgctc tactttaaaa ccttgatgac gtattgattg tgccaaatct
    20941 aaggcagcta gattagcctc tgatcctaat acagctatat acagatctat agtattttct
    21001 attggtaaag aaatcccttg cttatctaat attaagagca agcgttctaa tcctaatcca
    21061 aaaccaaatg ctggtgtttc tggtccacca aaataggata ctaagccgtc ataacggcct
    21121 ccagcacaga tcgttaattc attatcttca atctcagtga taaactcaaa aatagtatgg
    21181 ttataataat caagaccacg aaccatatta gtatcaatga tgtatggaat atttagagca
    21241 tcaagcatat gacacacagc atcaaagtgg gcttggcttg actcatctaa ataatctaaa
    21301 atagaaggag cattttcaac tgctagtttg tcttctggct ccttagagtc tagtacgcgc
    21361 aatggatttt cattcaaacg acgttgacta tccttagaca attgttcacg tatgggtgtt
    21421 aagtaatcaa taagagcttg gcgataggct tgacgacttt ctggacttcc taaactatta
    21481 agatgaagtt taacgttttt gatacctaaa tcttcaaata gatgatgtcc cattgcgatt
    21541 gtttcaacat cagttgttgg attatttgat ccaaagcact caacgcctac ttgatgaaat
    21601 tctcgtaaac gaccagcctg tgggcgctca tatcggaaca ttgatccaat ataatacatt
    21661 ttggttggtt tttgaacttc tggtgcaaat agtttatttt caacataaga acgtacaaca
    21721 ggtgctgttc cttctggacg aagcgtaatg tgacgatctc ctttatcatg aaaatcatac
    21781 atctcttttg taacgatatc cgttgtatcc ccaactgagc gactaataac ctcgtaatgt
    21841 tcaaacattg gtgtacgaat ttcatcataa tgatattgtt taaagagatt gcgtataaca
    21901 ttttcaacgt attgccactt tgctgattct ccaggtaaga tatcctgagt cccttttggt
    21961 ttttgaagtt tcataagttt acctcttcga ttagttttct ctttaaacat tctaccattt
    22021 tttcttatat tttaaacaag catatatcat ttgctttagt ttgatagtat tttcaccatg
    22081 aaattgtaaa aataaaaagt tttgtcaagt aactttactt tacaaaaaag atatgttatc
    22141 atagtgaagt tgatgaaaat caaaacttac acatcgaaag gaagaataag aaaatggcag
    22201 tacctgcacg tcacacttca aaagcgaaga aaaacaaacg tcgtacacac tacaaattga
    22261 cagctccatc tgtacaattc gacgaaacta ctggagatta ctcacgttca caccgtgtat
    22321 cactcaaagg atactacaaa ggacgtaaaa tcgctaaagc taacgaagct aaataataga
    22381 agggagatac catgcgcgta aatattacac ttgaacataa agaatctggt gaacgcttgt
    22441 accttacttc aaaaaacaaa cgtaacactc cagaccgtct tcaattgaaa aaatactcac
    22501 caaaacttcg taaacacgtt gttttcactg aagttaagta agggtttcag tacacgtcaa
    22561 tagacgtcaa aatattgatt ttaagcgatt tttgattttt tgaaactcat tatatcgcac
    22621 tacaaatcaa taaaaactct accttttact ctaccttttt tagataaata tatcatctgg
    22681 atattgaagt taagccctgc tatcatttcg atggcagggc tttttaatat ttgtacacta
    22741 acttcttatc tatatt
//
LOCUS       DATRQA010000016        21882 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.016, whole genome shotgun sequence.
ACCESSION   DATRQA010000016 DATRQA010000000
VERSION     DATRQA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21882
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.016"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..156)
                     /locus_tag="VBD27_000461"
     CDS             complement(<1..156)
                     /locus_tag="VBD27_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO7024496.1"
                     /translation="MIYDHLLNLTHYKDINPNLDLAIDYLLSHDLRNLDIGTYHISPE
                     VILMVQSN"
     gene            complement(169..999)
                     /locus_tag="VBD27_000462"
     CDS             complement(169..999)
                     /locus_tag="VBD27_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO7024497.1"
                     /translation="MKKKTFSAYNFLTALILCLLTVLFIFPFYWIMTGAFKSQPDTII
                     IPPQWWPKAPTLENFKALTVQNPALRWLWNSVFISIMTMFLVCCTSSMAGYVLAKKRF
                     YGQKILFSLFIAAMALPKQVVLVPLVRIINFMGIHDTLWAVILPLVGWPFGVFLMKQF
                     SENISTELLESAKIDGCGEIRTFINVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(1009..1896)
                     /locus_tag="VBD27_000463"
     CDS             complement(1009..1896)
                     /locus_tag="VBD27_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7024498.1"
                     /translation="MRTNKLKMRETMIAYAFLAPILLFFLIFVFAPMVMGFVTSFFNY
                     SMTQFTFIGLANYNRMFHDSIFMKSLINTVIIVIGSVPVVVFFSLFVAANTYEKNVFS
                     RSFYRCVFFLPVVTGSVAVTVVWKWIYDPMSGILNYILKSGHVIEQNISWLGDKHWAL
                     LAIIIILLTTSVGQPIILYIAAMGNIDNSLCEAARVDGANEMQVFWQIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIALISFAQFKILGNDVEY"
     gene            complement(1984..3300)
                     /locus_tag="VBD27_000464"
     CDS             complement(1984..3300)
                     /locus_tag="VBD27_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7024499.1"
                     /translation="MSIKKSVIGFCLGAAALSMFACVDSSQSVMAAEKDKVEITWWAF
                     PTFTQEKAKDGVGTYEKKVIKAFEKKNPNIKVKLETIDFTSGSEKITTAIEAGTAPDV
                     LFDAPGRIIQYGKNGKLADLNDLFTDQFIKDVNNKNIIQASKSGDKAYMYPISSAPFY
                     MAFNKKMLKDAGVLKLVKEGWTTSDFEKVLKALKNKGYTPGSFFANGQGGDQGPRAFF
                     ANLYSAPITDKEVTKYTTDTKNSVKSMKKIVEWIKKGYLMNGSQYDGSADIQNFANGQ
                     TAFTILWAPAQPKTQAKLLESSKVDYLEVPFPSEDGKPDLEYLVNGFAVFNNKDENKV
                     KASKKFITFIADDKKWGPKDVIRTGAFPVRTSFGDLYKGDKRMMKISKWTQYYSPYYN
                     TIDGFSEMRTLWFPMVQSVSNGDEKPADALKDFTQKANDTIKKAAK"
     gene            complement(3347..4045)
                     /locus_tag="VBD27_000465"
     CDS             complement(3347..4045)
                     /locus_tag="VBD27_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO7024500.1"
                     /translation="MPHLSKEAFKKQIKNGIIVSCQALPGEPLYTESGGVMPLLALAA
                     QEAGAVGIRANSVRDIKEIQEVTNLPIIGIIKREYPPQEPFITATMTEVDQLATLDIA
                     VIALDCTLRERHDGLSVAEFIQKIKRKYPKQLLMADISTFEEGKNAFEAGVDFVGTTL
                     SGYTDYSRQEEGPDIELLNKLCQAGIDVIAEGKIHTPKQANEINHIGVAGIVVGGAIT
                     RPKEIAERFISGLS"
     gene            complement(4292..5596)
                     /locus_tag="VBD27_000466"
     CDS             complement(4292..5596)
                     /locus_tag="VBD27_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO7024501.1"
                     /translation="MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADL
                     VKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAKINNIADINLIYPETTLTVTYDQKSH
                     TATSMKIETPATNAAGQTTATVDLKTNQVFVADQKVSLNTISEGMTPEAATTIVSPMK
                     TYSSAPALKSKEVLAQEQAVSQVAANEQVSPAPVKSITSEVPAAKEEVKPTQTSVSQL
                     TTVSPASVAAETPAPVAKVAPVRTVAAPRVASAKVVTPKVETGASPEHVSAPAVPVTT
                     TSPATDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENAGLQPHVAAYKEK
                     VASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGKNQALGNEVAQYSTQNMAANNISYVI
                     WQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK"
     gene            complement(5743..6642)
                     /locus_tag="VBD27_000467"
     CDS             complement(5743..6642)
                     /locus_tag="VBD27_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO7024502.1"
                     /translation="MNKWLVKASSLVVLGGMVLSAGSRVLADTYVRPIDNGRITTGFN
                     GYPGHCGVDYAVPTGTIIRAVADGTVKFAGAGANFSWMTDLAGNCVMIQHADGMHSGY
                     AHMSRVVARTGEKVKQGDIIGYVGATGTATGPHLHFEFLPANPNFQNGFHGRINPTSL
                     IANVATFSGKTQASAPSIKPLQSAPVQNQSSKLKVYRVDELQKVNGVWLVKNNTLTPT
                     GFDWNDNGIPASEIDEVDANGNLTADQVLQKGGYFIFNPKTLKTVEKPIQGTAGLTWA
                     KTRFANGSSVWLRVDNSQELLYK"
     gene            complement(6835..8382)
                     /gene="purH"
                     /locus_tag="VBD27_000468"
     CDS             complement(6835..8382)
                     /gene="purH"
                     /locus_tag="VBD27_000468"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO7024503.1"
                     /translation="MTKRALISVSDKSGIIDFAKELKNLGWDIISTGGTKVALDDAGV
                     ETIAIDDVTGSPEMMDGRVKTLHSNIHGGILARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDVTNDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     SQTTFKTRQRLAAKAFRHTAAYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEVTALEFAWKAIKYVKSNGIIITNDHMTLGLGVGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(8402..9154)
                     /locus_tag="VBD27_000469"
     CDS             complement(8402..9154)
                     /locus_tag="VBD27_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7024504.1"
                     /translation="MKLVKNLEIVESIFGDWDETIIWSCVQGIMGEVFVDSLDQPKSS
                     LAKLGRKSSFGFLAGQPTLFLLEVCSGEDIILVPQHKGWSDLIESTYGQNAHSFKRYA
                     TKKDTLFERSRLEKFVTQLPNGFELRVIDEKVYNSCLEKEWSQDLVANYATYQYYKKQ
                     GIGYVIYYQGNIIAGASSYSTYKNGIEIEVDTHPDFRRRGLATIVAAQLILTCLDKGI
                     YPSWDAHTRTSLNLSEKLGYEFSHEYIAYEID"
     gene            complement(9177..9725)
                     /gene="purN"
                     /locus_tag="VBD27_000470"
     CDS             complement(9177..9725)
                     /gene="purN"
                     /locus_tag="VBD27_000470"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO7024505.1"
                     /translation="MKIAVFASANGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAVVDLLDKHEIDLVCLAGYMKIVGETLLSAYEGRIINIHP
                     AYLPEFPGAHGIKDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPDVLDSLGIKRK"
     gene            complement(9892..10914)
                     /locus_tag="VBD27_000471"
     CDS             complement(9892..10914)
                     /locus_tag="VBD27_000471"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO7024506.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVSRTERAGVMGALGGFG
                     GVFDLSKTGVREPILVSGTDGVGTKLMLAIKYQQHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYIATGKNNPVKLEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLLDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVQAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            complement(10942..12396)
                     /locus_tag="VBD27_000472"
     CDS             complement(10942..12396)
                     /locus_tag="VBD27_000472"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO7024507.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPSFMGKVKE
                     ALSTVKGGFAYLLMTEDKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEETSFYIQKVK"
     gene            complement(12630..16355)
                     /locus_tag="VBD27_000473"
     CDS             complement(12630..16355)
                     /locus_tag="VBD27_000473"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEO7024508.1"
                     /translation="MNKRIFVEKKADFDIKSASLVKELTHNLQLTSLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLLGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPSLDFFETYKADDFAAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQATYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDTFIA
                     ACNKENIDAVVVATITAKPNLVMTWDGETIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEVRTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFDQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFETIQAQHKIT
                     AASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADSVKIG
                     QTQAAFTLTVNGNDLAGASLLSVFEGKLEEVYPTEFEQADALEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEAAIADSVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNKKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLVGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQALFASAVKYFTGK"
     gene            complement(16478..17182)
                     /locus_tag="VBD27_000474"
     CDS             complement(16478..17182)
                     /locus_tag="VBD27_000474"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO7024509.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNMAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHMKFLGIVNDEEIAYLKGETRRINEL
                     LKDWFAQIGLNLIDFKLEFGFDKDGKIILADEFSPDNCRLWDAEGNHMDKDVFRRDLG
                     SLTDVYQVVLEKLIAL"
     gene            complement(17306..17545)
                     /locus_tag="VBD27_000475"
     CDS             complement(17306..17545)
                     /locus_tag="VBD27_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085641.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7024510.1"
                     /translation="MSRDEVFEKMLELLRQQLGDPQLDITPESSLHDDLAIDSIALTE
                     FIINLEDVFHLEIPDEAVEHMSSVQQLLDYIIEHK"
     gene            complement(17556..18548)
                     /gene="plsX"
                     /locus_tag="VBD27_000476"
     CDS             complement(17556..18548)
                     /gene="plsX"
                     /locus_tag="VBD27_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO7024511.1"
                     /translation="MKKIAVDAMGGDYAPKAIVEGVNQAISDFSDIEVQLYGDQKKIE
                     KYLTVTERVSIIHTEEKINSDDEPAKAVRRKKQSSMVLGAKAVKDGVAQAFISAGNTG
                     ALLAAGLFVVGRIKGVDRPGLMSTMPTLDGVGFDMLDLGANAENTASHLHQYAILGSF
                     YAKNVRGIEVPRVGLLNNGTEETKGDSLHKEAYELLAAEPSINFIGNIEARDLMSSVA
                     DIVVTDGFTGNAVLKTMEGTAMNIMGSLKSSIKSGGVKAKLGALLLKDSLYQLKDSMD
                     YSSAGGAVLFGLKAPIVKCHGSSDSKAVYSTLKQVRTMLETQVVDQLVDAFTDE"
     gene            complement(18626..19504)
                     /locus_tag="VBD27_000477"
     CDS             complement(18626..19504)
                     /locus_tag="VBD27_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7024512.1"
                     /translation="MELIFRIFEMKKTLYITNKLFFANWFVPVIGAVLIQVIGIIVSR
                     RLVVKLLPDDYKFNLYIALLLFIPILASILLFLIYFQKRDFTSLGFTKDNNVLTTISG
                     FITGSLLMFFIYICHWLSGFVEISSSNSFSFGPFFFLLLAYFIQSLTEEILFRGYVMT
                     TVTKFKGSFAGVLCNSMLFSFIHFRNYGITAIALFNLFLLGIIFSILFNMTKNILFVT
                     GVHTAWNFTMGCVLGNKVSGGYSPVSLFRITENSSFAIWSGGDFGFEGGVLCSLILVG
                     CLAYFSLERVKVSVRY"
     gene            complement(19567..20328)
                     /gene="recO"
                     /locus_tag="VBD27_000478"
     CDS             complement(19567..20328)
                     /gene="recO"
                     /locus_tag="VBD27_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO7024513.1"
                     /translation="MRVSQTYGLVLYNRNYREDDKLVKIFTETEGKRMFFVKHASKSK
                     FNAVLQPLTIAHFILKINDNGLSYIDDYKEVLAFQETNSDLFKLSYASYITSLADVAI
                     SDNVADAQLFIFLKKTLELIEDGLDYEILTNIFEVQLLERFGVALNFHDCVFCHRAGL
                     PFDFSHKYSGLLCPNHYYKDERRNHLDPNMLYLINRFQSIQFDDLQTISVKPEMKLKI
                     RQFLDMIYDEYVGIHLKSKKFIDDLSSWGSIMKSD"
     gene            complement(20318..21493)
                     /locus_tag="VBD27_000479"
     CDS             complement(20318..21493)
                     /locus_tag="VBD27_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7024514.1"
                     /translation="MTLEKRFNKYLDRIEVSLIRQFDQSISDIPGMVKLTLGEPDFTT
                     PDHVKEAAKSAIDANQSYYTGMSGLLALRQAAADFAKDKYNLTYNPDSEILVTIGATE
                     ALSASLIAILEAGDVVLLPAPAYPGYEPIVNLVGADIVEIDTRENDFRLTPEMLETAI
                     IQQGEKLKAVLLNYPTNPTGITYSRQEIAALAEVLKKYDIFVISDEVYSELTYTGQQH
                     VSIAEYLPNQTILINGLSKSHAMTGWRVGLVYAPEAFTAQIIKSHQYMVTAASTISQF
                     AGVEALSVGKNDTLPMRQGYIKRRDYIIDKMSKLGFKIIKPSGAFYIFAKIPDSYPQD
                     SFKFCQDFAYQQAVAIIPGVAFGKYGEGYIRLSYAASMEVIETAMARLKVFMESYEG"
     gene            complement(21601..>21882)
                     /locus_tag="VBD27_000480"
     CDS             complement(21601..>21882)
                     /locus_tag="VBD27_000480"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-phosphate from D-ribose 5-phosphate and ATP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEO7024515.1"
                     /translation="AGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQNSAIEKL
                     IVLDTIYLPEERLIDKIEQISIAELIGEAIIRIHEKRPLSPLFEMNKLK"
BASE COUNT         6750 a         4672 c         3761 g         6699 t
ORIGIN      
        1 atttgattgg accatgagta tcacttctgg gctaatatga taggtgccta tatctaaatt
       61 ccttaaatca tgacttagta gataatcaat cgctaaatct aaattaggat taatatcttt
      121 ataatgcgtt aaattcaata aatgatcgta aatcataata aatcctacct atcctttaac
      181 agctcccatt gtaatcccct gagtaaagga tttttggaaa actaagaata ctgtcacaat
      241 aggaacagca gctaaggctg cacctgccat aattaagcca tagtttgttg ccatttcagc
      301 ctgcatagta gctaccccaa gagaaatggt cagattattt ctagaagtta gcataactaa
      361 ttgcataaaa tagtcattcc aagtattgat aaatgtgaaa atagctaggg cagcaaatcc
      421 aggtttgaca attggaaatg ctacattaat aaatgttcga atctctccac aaccatcaat
      481 tttagctgat tccagtaatt ctgttgaaat attttcagaa aactgtttca tcaagaagac
      541 cccaaaaggc caaccaacaa gaggtaaaat aaccgcccat aaggtatcat gtatccccat
      601 aaaattgata attctaacta atggcactaa aacaacttgt ttaggtaaag ccatagcagc
      661 aataaaaagg gagaacaata ttttttgacc gtaaaatcgt ttcttagcta aaacatagcc
      721 tgccattgac gatgtacagc aaactaggaa cattgtcata attgagataa agacactatt
      781 ccaaagccat ctcaaagctg ggttttgtac agttaaagct ttaaaatttt ctaaggtcgg
      841 tgctttgggc caccattgtg gggggatgat aatagtatca ggttgagatt taaaagctcc
      901 tgtcataatc caataaaatg gaaagataaa aagcactgtc aaaagacaaa ggataagagc
      961 cgttaaaaag ttataagcac tgaatgtttt ctttttcatg ttttcctcct aatattccac
     1021 atcatttcct aagatcttaa attgagcaaa acttattaaa gcaatcatta ctgctaggaa
     1081 tactcccata gtattagcat agccatattc tgatagttta aaggcttttt cataaagata
     1141 atacatcaaa gtgcttgttg aataatttgg tccaccagat gttaggagct ggattaaggc
     1201 aaaacattga aatgaattaa ttgttgttat aactgcaata tagagtgtag ttggtaagag
     1261 actcggccat ttaatttgcc aaaaaacttg catttcattg gctccatcaa cacgcgcagc
     1321 ttcgcaaaga gaattatcaa tatttcccat cgccgcaata tatagaataa taggttgacc
     1381 aactgaggtc gttagcaaaa taataataat agcaagtaat gcccagtgct tatcaccaag
     1441 ccagctaata ttctgttcaa tgacatgacc tgatttcaag atgtaattta aaattccgga
     1501 catagggtca taaatccatt tccaaacaac tgtgactgca acactaccag ttactactgg
     1561 caagaagaag acacaacggt agaaagagcg tgaaaaaaca tttttttcat aagtattcgc
     1621 agcaacaaaa agtgagaaga aaaccactac tggcacagag ccaatcacaa tgattactgt
     1681 atttatcaag gatttcatga aaatagaatc atgaaacata cgattgtaat tagctaaccc
     1741 aataaaagtg aactgtgtca tagaataatt aaaaaaacta gtgacaaagc ccataaccat
     1801 cggtgcaaaa acaaaaatta agaaaaataa gagaataggc gctagaaaag cataagctat
     1861 catcgtttcc ctcatcttta acttattagt acgcatacgg tactctcctt tacatcttaa
     1921 cttgactaaa aactcctctt ctatcccaat atgtgattga ccatagaaaa ggagtctcct
     1981 taattattta gctgcttttt taatggtatc atttgctttt tgagtaaagt ctttcaaagc
     2041 atctgctggt ttttcatcac cattggatac agattgaacc attgggaacc ataaggttct
     2101 catttcagaa aatccatcga tagtgttgta atatggtgaa taatattgag tccattttga
     2161 aatcttcatc atacgtttat cacctttata aagatcccca aatgatgttc taactgggaa
     2221 agcacctgta cgtataacat cttttggtcc ccatttttta tcatcagcaa taaaagtgat
     2281 aaatttctta gaggctttta ctttgttttc atctttatta ttaaagaccg caaaaccatt
     2341 aacaaggtat tctaaatctg gttttccatc ttctgatggg aatggcactt caaggtaatc
     2401 cactttactt gactctaata attttgcttg agtttttggt tgagctggag cccataggat
     2461 agtgaaagca gtttgtccat tggcgaagtt ttgaatgtca gctgagccat catactgaga
     2521 cccattcatc aagtagcctt tcttaatcca ttcaactatt tttttcattg attttacaga
     2581 atttttagtg tcagtggtat attttgttac ttctttatct gttattggag cactataaag
     2641 attagcaaaa aatgcacgtg gtccttgatc tcctccttgc ccgtttgcaa agaatgaacc
     2701 tggtgtatag cctttatttt ttagtgcttt tagtactttt tcaaaatcac tagtagtcca
     2761 accttctttt acaagtttca aaactcctgc atctttaagc atttttttat tgaacgccat
     2821 ataaaatggg gcagaactta ttggatacat gtaggcttta tcgccagact tagaagcttg
     2881 aatgatgttc ttattattga catccttaat aaattggtct gtaaataaat cattcaaatc
     2941 tgctaattta ccatttttac catattgaat aattcgccct ggtgcatcaa aaagcacatc
     3001 aggtgctgtc cctgcttcaa ttgctgtagt gattttttca gatccagatg tgaaatcaat
     3061 tgtctctagt tttactttta tattaggatt tttcttttca aaagccttga tgactttttt
     3121 ctcataagta cctactccat ccttagcctt ttcttgagta aaggttggaa aagcccacca
     3181 cgtaatttcg actttatcct tctcggcagc cataacagat tgactactgt ctacacaagc
     3241 aaacattgat aatgctgcag ctccgaggca aaaaccaatc acactttttt tgatactcat
     3301 aactattccc cttttttatt tttattttga tttttgagta acacttttaa ctaagtcctg
     3361 agatgaaacg ctccgctatt tcttttggtc tagtgatagc acccccaact acaattcctg
     3421 caacacctat atgattaatt tcattagcct gcttaggagt atgaatttta ccttccgcaa
     3481 tcacatctat accggcttga caaagcttat taaggagttc tatatccggt ccttcttctt
     3541 ggcggctgta atctgtgtat cccgatagag ttgtacccac aaaatcaact cctgcttcaa
     3601 aagcattttt gccttcttca aaagtactta tatcagccat taacaactgt ttaggatatt
     3661 tcctttttat cttttgaata aactcagcta cactcaaacc atcatgacgc tctctaagtg
     3721 tacaatctaa ggctattact gcaatatcta aagtagctaa ttgatccacc tctgtcatcg
     3781 tagcagtgat aaatggttct tgtggaggat attcacgttt aataatgccg atgataggta
     3841 aattagtaac ttcttgaatt tccttaatgt cgcggacact attggctctt ataccaaccg
     3901 ctcctgcttc ttgagctgcc aaagctaaaa gaggcataac acctccactt tcagtataaa
     3961 gaggctcccc aggcaaagct tgacatgaca caataatgcc attttttatt tgctttttaa
     4021 aagcttcttt acttagatgt ggcatattct cacttcctta tcttttttaa ttttagatat
     4081 ctacaaacct tattatatct atttttaaag cgttttcaat aatctttaca gacttagatt
     4141 ttagttttaa cgttttgatt tagtcacggt taacgtgaaa gtagtttcag acttatagct
     4201 taatctcctc aatttaataa aaattatata agaaccttga aagtctattc aaggcatata
     4261 aaaaagaagc caaatagctt ccttttttat attatttgtt aaatgatacg tgaacgtggt
     4321 catagtggtt ggcagtaacg ccaccacgat ctggcattgc attccaagta ttagcaggtc
     4381 cataaatact atttgtattt gaataaaact tttgttgcca gataacatat gaaatgttat
     4441 ttgctgccat attttgtgta gagtactgtg caacttcatt accaagtgct tggtttttac
     4501 ctacaataaa gtcaactgct aaacctttac catgatcacc tggatctccc gcacggtatg
     4561 tactgaattc attaactcca taagttgacg ctactttttc tttataagct gcaacatgag
     4621 gttggagccc tgcattttca ggatgtgcag ctactgcatt tgttgttgaa gctggttgtg
     4681 ctaccggtgt tgctgttgga gctttttgtg ctaccggaac gctcttaact tcagtcgctt
     4741 gtaacttact gtctgtagct ggtgaagtcg tagtcacagg aactgctgga gctgatacat
     4801 gctctggtga tgcaccagtt tctactttag gagtgactac tttagcactt gccactctag
     4861 gggctgccac agttcttacc ggtgctactt tagctactgg agctggtgtt tcagcggcaa
     4921 cagaagctgg tgatactgtt gttaactgac tgactgacgt ctgagttggt ttaacttcct
     4981 ctttagctgc tggaacttct gaagtaatcg acttcacagg agctggtgat acctgttcat
     5041 tagctgctac ttgactaaca gcttgctctt gtgctaatac ttcttttgat ttcaaagctg
     5101 gcgcagaaga atatgtcttc attggcgaaa caatcgttgt tgctgcttct ggtgtcatac
     5161 cttccgaaat tgtattgaga gaaacttttt ggtctgcaac aaaaacttga ttggttttca
     5221 aatcgacagt agctgttgtt tgaccagcag catttgttgc tggtgtttct attttcattg
     5281 aagtggcagt atgactcttc tgatcgtaag ttactgtcag tgttgtctca ggataaataa
     5341 gattgatatc tgcaatgtta ttaatttttg ctaagacatt catatcaatt gacattgctt
     5401 ctgaaataac gcttagtgta tcaccatatt tcacagtata tgatgattta ttgtcttgct
     5461 ttaccaaatc agcctttacc tctgaaacag tacgtgctgt ccacgtcgta tctgtttctt
     5521 gtgcttgaac acttgcgact gataatagcg aagctgccat tgtcgatgtc aatagtacct
     5581 ttttattcat tttcattgct tcaattccta tttccttttt attagtccga gactataata
     5641 tcacacttta ctggactata aaattatttt cagacggttt aaagaaattg tacttagtta
     5701 gtaacaaaac tgtcatttaa aacaatgaat caatacctca aactatttgt aaagcagttc
     5761 ttgactgttg tcaacgcgaa gccaaactga actaccatta gcaaagcgtg tcttagccca
     5821 agttaaacca gctgttcctt ggatgggttt ttctacagtc ttaagagttt taggattaaa
     5881 gataaagtaa ccaccttttt gaagaacctg gtcagctgtc aaattaccat tagcatcaac
     5941 ctcatcaatt tctgatgctg gtataccatt atcgttccaa tcaaacccag tcggcgttag
     6001 ggtgttattt ttgactaacc aaacaccatt aaccttttgt aattcatcta ctcgatacac
     6061 ttttaattta ctagattgat tctgtacagg agctgattgt aatggcttaa tgcttggagc
     6121 tgatgcttgc gtttttccac taaaggtcgc aacgttagca attagtgacg ttggattgat
     6181 acgtccatgg aaaccatttt gaaaattagg gttagctggt aaaaattcaa aatgaaggtg
     6241 aggtcccgtc gccgtaccag ttgctcctac gtaaccgatg atatctcctt gtttgacttt
     6301 ttccccagtc ctagccacca cacgtgacat atgagcgtaa ccactatgca ttccatccgc
     6361 atgttgaatc atgacacaat ttcctgctaa gtctgtcatc caagaaaagt tggctccagc
     6421 tcctgcaaat ttcacagtac catctgccac tgccctaata atcgttccag tcggaacagc
     6481 ataatccacc ccacaatgtc caggataacc attgaaacct gttgtaattc taccattatc
     6541 aattggacgg acataagtat ccgctaaaac tcgggaaccc gcagataaaa ccataccacc
     6601 taaaacaact aaggaacttg ccttaactaa ccatttattc aagaaaaacc tccaaataga
     6661 attaatataa tgaagtgctc aaacacttgc aattaactta ttcgaaaatt taaatcaaaa
     6721 tatcaaaaaa agtactcacc ttgatgagta cttaggaggt ttaataaaaa gaaaagtttt
     6781 taggatatac gtagtatacc tgagttaact taagaaaacc taaactacta ttttttaatg
     6841 tctaaaatgt ctcacgcctg tgaagatcat ggtcaagcca tgtttgtttg cggcgtcaat
     6901 agattcttga tcacgaactg aaccacctgg ctggatgatt gctttgatgc ctgcggcagc
     6961 tatttcttca atgttgtccg caaatgggaa gaaggcatct gatgctagaa cggcaccgtc
     7021 aaggtggtcc ttagcctgct cgatagcaat cttgactgag ccgacacggt tggtttgacc
     7081 tacacccagt ccaagcgtca tgtgatcgtt agtaataata atcccgttag acttaacata
     7141 cttgatagcc ttccaggcaa actcaagggc agtcacctct tgttctgttg gctggcggtc
     7201 tgtcaccact tgccagtcag atggattttc agccacaacg tcttggtttt gcaccaaaag
     7261 tccaccaact acgccagtgt actcagcttc cacttcgctg gcagcttggg catcaaacgg
     7321 caactcaagg atacgcaagt ttttcttttt atttgtgaga atagctagcg cttcttctga
     7381 gtatgatggt gcgatgatga tttccaagaa gatagggtgc atcttctcag ctgtcgctgc
     7441 gtcaacttca cggttaagga caacaattcc accaaagatt gaaactggat cagcttcata
     7501 agcgtaatcc caagctgtct caatatcatc agcctgtccg ataccacatg ggttcatgtg
     7561 tttgagggca acaaccgttg gactgtcttt gaaatcgcgg ataatacgga ttgctgcatc
     7621 agcatcacgg atattattga aggacaattc ttttccattg agctgtttag ctgaagcgat
     7681 tgagtagtct gttggtaagg ctttttggta gaaatcagcg tcttgctgtg gattttctcc
     7741 gtaacgcata gcctgtttaa gatcataagt gatggtcaat ttttcaggct tagcctctcc
     7801 cacttgagct gtgaagtact cagcaatcaa agcgtcgtag gctgccgtat gacggaaggc
     7861 cttagctgcc aagcgttgac gagttttaaa tgtcgtctga ctagcgtcag ccaattctcc
     7921 caaaacagtg gcatagtcag ctggatcaac cacaacggtt acgctagcgt ggtttttagc
     7981 agctgagcga agcattgatg gaccgccgat gtcgatgttt tccaccgcca aatcgttggt
     8041 cacgtctggg cgaaggatag tctccttgaa gggatagagg ttgacaacca cgaggtcaat
     8101 caactcgatg ttgttgtcct tagcagcctg aaggtggctg tcagcgtcgc gacgagccaa
     8161 aatcccaccg tgaatgtttg agtggagggt cttaacacga ccatccatca tttctgggga
     8221 gccagttaca tcgtcaatag caatggtctc aacaccagca tcgtcaaggg caaccttagt
     8281 cccaccagtt gagataatat cccaacccaa gtttttcaat tcttttgcaa agtcaataat
     8341 tcctgatttg tcagaaactg agattaaagc acgtttagtc atgagttctc cttttcttac
     8401 ttcaatctat ttcataggct atgtattcat gagaaaattc ataaccgagt ttttcagata
     8461 aattcaatga agtccttgta tgagcatccc agctaggata aattccctta tctaaacaag
     8521 ttagtatcaa ctgagctgca actattgttg ctaaaccacg ccgacgaaaa tctggatgcg
     8581 tatctacttc tatctcgatc ccatttttat aggttgaata agatgaagct cctgcaatga
     8641 tattcccctg ataatatata acataaccta taccttgttt tttatagtac tgataagtag
     8701 cgtaatttgc tactaaatcc tgtgaccatt ccttttctaa gcaagagttg taaactttct
     8761 catcaataac acgtaattca aaaccatttg gcaactgagt aacaaacttc tctaacctac
     8821 ttcgctcaaa caaagtatct tttttcgtcg cataacgctt aaaagaatgg gcattctggc
     8881 cataagttga ttcaatcaaa tctgaccatc ctttatgctg aggaacaagg ataatatcct
     8941 ccccagagca aacttctagt agaaataaag taggctgacc tgctagaaag ccaaaagatg
     9001 atttccttcc caattttgcc aaagaagatt ttggttggtc taaactatct acaaaaactt
     9061 ctcccataat accttggaca cacgaccaga ttatggtctc gtcccaatca ccaaaaatag
     9121 actctactat ctctaaattt ttcactagtt tcattagctt attataacaa attttactat
     9181 tttctcttta tgcccaaact atccaaaaca tctggataga gttgatattc ggtttcatgg
     9241 atgcgggttt caaagctttc taggctatcg tctgctaggc gtggcacgcg cacttgttgg
     9301 atgacctgac cggtatccac accagagtcc acccaatgga tggtcacacc agactggtca
     9361 acacctgcct cccaagcatc cttgataccg tgagcacctg gaaattcagg cagataggct
     9421 gggtgaatat tgatgatacg cccttcatag gctgagagca aggtttctcc gacaatcttc
     9481 atgtagcccg ccagacagac caagtcaatc tcgtgtttgt ccaacaaatc aacgacggct
     9541 tgctcgtaag ccgccttatt ctcaaactct ttgagttcaa aagcgaagct tggaatggct
     9601 aagttctgag cacgctctaa aacataggca tcacgatgat ctgaaaagac aaaactaact
     9661 ggaaactgct ctgctatgac ctgaaagttg gaaccattag cagaagcaaa aacagcgatt
     9721 ttcatattcg atacaaaggg atgattcggc ttgccgaaaa atttctagaa aaataggaaa
     9781 acgaacaacg acgcttgcgt ctaagtcgtt ttatcttttt tcccagaaat ttaatcccgt
     9841 gttcagttag gcaaactaac taaacactcc tttcttataa aatttagtga cttatttaat
     9901 caccacacta tcgtctgctt tcttgatgat acgaccgatt tcataaactg gttcgtccaa
     9961 gagctctttg acgcggttaa cattttcagg actaacagct agcataagac cgacacccat
    10021 attgaagatt tcaaacattt cttcgtgctt gatgtcacca tatttttcaa gcgccttgaa
    10081 aatcggaagt actggcacct tatcctcatc gatttccgca gccaaatcat ccgcaaacat
    10141 acgaggaaca ttctcgataa agccaccacc cgtgatgtgg ccgataccgt tgactagttc
    10201 ttccttgatt aatggcagag ctgcttgaac atagatacga gttggctcaa gaaggacatc
    10261 cttgagttgt ttaccttcaa gctctggaag cagctcttta ccagtatagt cagcaaagac
    10321 acgacgcacc agcgaataac cgtttgagtg gataccactt gaagcaagtc ccaaaagaat
    10381 atcgccttcc tttactttgg aaccatcaag gagctgagat ttttcagcca caccaacagc
    10441 aaagccagca aggtcataat catcttcgcc atacatccca ggcatttcag cggtctcacc
    10501 tccaatgagt gctgctcctg cttgaacaca accttctgca acgccagaaa caacttcttc
    10561 aagtttgact gggttatttt ttcctgtcgc aatataatct aaaaaatata atggctcagc
    10621 acctgctgca ataatatcat tgacacacat ggcaacacaa tcttggccta tggtgtcatg
    10681 ctgttgatat ttaatcgcaa gcatgagttt tgtgccaaca ccgtctgttc ctgaaaccaa
    10741 aataggctct ctaactcctg ttttactcaa gtcaaacacc ccaccaaagc cacctagagc
    10801 tcccatgact cccgcacgtt ctgtacgaga aacgtgtttt tttattcttt caaccacttc
    10861 ataaccagct tcaacatcaa caccagattt tgcataagca tttttttcag acatgttttc
    10921 tccttatata attagcagag cttatttcac tttttgaata taaaaacttg tttcttcttc
    10981 taaacttctg aggtattctt cttcataatc ataaagcggt gttggataat gtccatcaaa
    11041 gtaagctacg cataaaccac catttggtgc ttttgtttcg agtccaattg attcaatcaa
    11101 cccatctagt gagagatagg tcagactatc tgcgccaata atatcacaca cttcgtcagc
    11161 agcgtgattt gctgaaatta attctcgtct tgtttgaata tcaataccat aaaaacaagg
    11221 atactttaat tctggactag ctatagcaac atgtacttca ctagctcctg cttctcttaa
    11281 taatccgaca atccttctag aagtcgttcc tcttacaatt gagtcatcaa tcataacaac
    11341 gcgctttcct ttgacaacac cagataccgc tgatagtttc attcgaacac cttgttccct
    11401 taattcttgt gtcggttgaa taaaggttcg ctgcgtatac tgatttttta caagacccat
    11461 ctcatttggt aatccggatt cttcagcaaa gcccatagca gccgataagg acgaatttgg
    11521 gacaccaatt acaatatcag catcctgttt aaattcttgt gcaagacgct ttcccatatt
    11581 ttttcgagcc gtatgaacat taacaccatg tatagttgaa tccggccttg caaaatagac
    11641 atattccatc gaacaaattg ccagttgcgt ttcatcagta taacgatcac attgaatacc
    11701 actatcgtct ataaggataa cttcgccagg ttcaacatct ctaacccatt ttgcgcctac
    11761 cacctcaaaa gcacaggtct cactggaaat aacccaagca ccattttgca tttgtccaat
    11821 tgacaaagga cgaaaggcat tagggtcaag agcagcaatt aatttatctt ctgtcatcag
    11881 tagataggcg aagcctccct ttacagtgct taaagcttct tttaccttcc ccataaaact
    11941 tgggttatgg cttcgacgaa tcaagtgcat caaaatttca gtatctgagg aggcattgaa
    12001 aattgcacct tgcttttcta attctttcct taaagaaata gcatttgtca aattaccatt
    12061 atggcataaa gcaaattgcc cgtcatgaaa tttataaaga aaaggctgaa tattgcgaat
    12121 atctgcagaa cctgcagtag cataccgaac atgtccaata gccgcattcc cagttaaatt
    12181 atctaattca gattgattct taaaaacttc agaaaggagc ccaacatttc gataaccata
    12241 gagtttccca ttatcattcg aaacaatacc agcaccttct tgaccgcgat gttgaaggct
    12301 atgaagccca aagtaagtga cttgagctgc ctgaggatgc ccccagatac caaagactcc
    12361 acattcttca tttagagatt ttacttcgta tgtcattctt ttttcctaat tctttttgta
    12421 tctcatctta agtagctttg atgattttct caattttgga tctatcgtca ccaatgccaa
    12481 catcaaaata gaaaatctat caagctattt tcaagctaat cattgagtct tagcctcaac
    12541 ggctcccaaa taccactacg acagaaagat acagttaagc tcgcttcgct cttgtttcct
    12601 tatctgtttt atgaaaaaat ttgaaaaaat tattttcctg taaaatattt tactgcacta
    12661 gcaaacaagg cttggtcttt gttaccaggg atattttgga agaggccgtc ttcccagcgt
    12721 tctgagtgcc ccatcttacc gatgatttga ccattcttgc tggtaatccc ttcgatggca
    12781 ttgacagagc catttggatt gtatttagaa tccatagatg gttgtccgtc aaagtccaca
    12841 tattggctcc agatttgacc attgtctctt agctctgcaa attcagaagc gctgacaaca
    12901 aatttacctt caccgtgtga aactgggatg gcatgaatat cgccgacctc aactcctacc
    12961 aaccaaggtg agttggtatt tgcgatacga gtctcaacca tcttggcaac gtgctggttg
    13021 gcatcgttat agaagagggt tggacttgtc tcaccagctt cctcgaagtt tccgtatgga
    13081 agaagacctg atttaacaag ggcttggaaa ccattacaga taccgatgat aaggccacct
    13141 ttttcgatga agctgtcaat agctgcgcga accttcttgt taagcaagat attgacgata
    13201 aacttagcag acccatctgg ttcatccgct gctgagaatc ctccagcaaa gaagatgata
    13261 tttgccttag caatattagc gaccattgtg tcaactgagt cagcaatagc agcctcattc
    13321 aaggttacaa atggtaccaa gttgacacta gctccaacct gttcaaaggc cttagctgaa
    13381 tcatattctg agttggtacc agggaagact ggaatgtaaa ccactggttt ttcaattgtt
    13441 tccttagcct tgataacagt atctgatacc acagcaggaa cttcttcaag agcgtctgcc
    13501 tgttcaaact ctgttgggta aacctcttcc aatttgcctt cgaagactga tagaaggctt
    13561 gccccagcaa ggtcatttcc attgacagta agtgtaaagg ctgcctgagt ttgaccgatt
    13621 ttcaccgagt cagcaatttc ctcagctgat gtaaagacaa agcctccgag ttgagctgtc
    13681 aagctactgt caagctctgc aatttcaaca gaggccccga tacggttacc aaaagtcatg
    13741 agagcaagac tttctaggac accaccgtat ttaacagctg aagcagcagt aatcttatgt
    13801 tgagcttgaa tagtctcgaa ctggctaaag ttagccttga taaggtcaaa atcaatatct
    13861 tctgaaatag cttgacctgg aatgtagtag atattctcac cagccgcttt aaactcagga
    13921 gagagaacct tgcggctgtc cgctgttgtc acaccgaaag ctaccaaggt tggcggtact
    13981 gtcaattctt cgaaagtacc agacatagag tccttaccgc cgattgatgg caatccaagt
    14041 tgaatctgag cctcaataga accaagaaga gctgatactg gctgatcaaa acgctctgcc
    14101 tgtttatcca tacgctcgaa atactcttgg taagagaaac gtgcacgaga ccagtcagca
    14161 cccgttgcta ccaagcgagc tgtcgcttca atgacagcat aggcagcacc gtggtaaggt
    14221 gaccattctg caatataagg attgtaacct tgagccataa cagatgcagt tgttgtgaca
    14281 ccgtgttgaa ctggcaattt ttgaacagag ctttctgttg gtgtgatttg gtaacggcca
    14341 ccgattgggt ggttaacggt tgagcgacca acagatgagt caaagatagt ttggagacct
    14401 ttttgactag cgtggttgag gtcagacaag accttaagcg tatccgcttc aagtgtctct
    14461 gctgatgttg tgcgtacctc tggaactgtc aagtctttgt caacgacttt agcatcaaca
    14521 acgacacgga caccgttggt atcaaggaaa cggcgttcca agtcaacaat tgtttcacca
    14581 tcccaagtca tgacaaggtt tggttttgca gtaatagtag caaccacaac ggcatcgata
    14641 ttttccttgt tgcaggctgc gatgaaggta tccacgtcac taggacgaac aacgactgac
    14701 atacgctctt gtgattctga gattgcaatt tcagtaccat taagaccttg gtatttaagc
    14761 ggtaccttgt ccaaatcgat ttcaagaccg tcagccaatt caccgatggc aacacagaca
    14821 ccacctgcac cgaagtcatt tgatttcttg attaggcgag tgacattacc atcacggaaa
    14881 aggcgttgaa tcttacgttc ttcgatggca ttcccttttt gtacctctgc gccagctgtt
    14941 tccacagatt caaccgtttg aaccttagat gaacctgtcg caccgccgac accgtcacga
    15001 cctgttttac caccgagcaa gatgaccaca tcgcctgctt ctggtttttc acgaaccaca
    15061 ttttccttag gtgcagcacc aaccacagct ccaagctcca tgcgtttggc tacaaagcca
    15121 gggtgaaagt actcacgcac ataagttgtc gcaagcccaa tctggttacc atatgaagaa
    15181 tagccgtgcg ccgcagtttt agaaataacc tgttgcggca atttaccagc acgtgtttcc
    15241 gcaatcggag tcgtaatatc gcctgcgcct gaaatacgca tagcctgata aacgtatgaa
    15301 cgtcctgaca acgggtcacg aatggcacca ccgatacaag ttgctgcgcc accaaatggc
    15361 tcaatttctg ttgggtgatt gtgagtctca ttcttaaaca tgaggagcca aggctctttt
    15421 acaccatcaa catctacttc aatttcaact gagcaggcat tgatttcatc tgagacttcc
    15481 atatcatcca gtcgtccatt ggcacgttca taacgaccaa agatagtcgc catatccata
    15541 agtgtctgcg gcttttcaga acgaccaagt tcatcacgca tggcgatata tttgtcataa
    15601 gtcgcctgca attgtttttg gaatttagaa gctgaaaagt cgatgttttt caactcagtt
    15661 tcaaaggttg tgtgacggca gtggtctgac cagtaagtat ccaaaacttt caactcagtt
    15721 tcagttggca cacgcccgat tgatttgaaa taatcttgga tgaagagaag atcatcgacc
    15781 tccatagcca agccctgctc tgccttatag gccgcaaaat cgtcagcctt ataagtttca
    15841 aagaaatcaa gactagggat tgtcttatca gacacagaga aagcctgctc ttcaagcggc
    15901 aaggtaatgt ccttgaaacg tgaatcaaca ggattcaaaa gatagttctt aacggcttca
    15961 agctctgctt ctgcaatatc cttattgacc aagtaaagct gggctgtatt gaccttaacc
    16021 tgactgtcac ttccaagcaa tagcaaagct tcttgcgaac tagcagcacg ttggtcaaat
    16081 tgaccaggaa gcgcctcaat ggcaaagaag gcaaccttat caagctccgc agtgatttca
    16141 gtttccgtca aaagacagtc tgtcacctgc tcagagaaaa tatgcttctc agcacgcgcc
    16201 agcaaatcct cagccaaatt gaagacatca tagacctgca caatacgcaa atccttcaaa
    16261 gaagtcagtt gtagattatg cgtcaactct ttcacaagac tagccgattt aatgtcaaag
    16321 tcagcctttt tctcaacaaa aatacgttta ttcatataag ataccaagtc cgacaaggga
    16381 gcgactgaaa aataggagga tatctgaaat tgagatttca gtaagcccaa tcttttttct
    16441 gagtttcccg aacggattca attccttcct cttctaatta caatgctatc aacttctcaa
    16501 gtacaacttg atagacatcg gtcaagctac caagatctct acgaaaaaca tccttgtcca
    16561 tatgatttcc ttcagcatcc catagacggc agttgtcagg tgagaactca tcagccaaga
    16621 tgattttgcc atccttgtca aatccgaact ccaacttgaa gtcaatcaag ttaagcccaa
    16681 tctgagcaaa ccaatctttc aagagttcat taatacggcg agtctctcct ttcagataag
    16741 caatttcttc atcattaaca atacccaaaa acttcatatg ctcatcattg ataaaaggat
    16801 cattcagatc atcatttttg tagtaaaatt ccacaattgg agtttcaaga acgcgtcctt
    16861 cttccactcc aaaacgtttt gaaaatgagc ctgctgtcac attccgtaat accacctcaa
    16921 gtggaataat atcaactttt ttattcaatt gttcgttttt tgaaatttgc tcaatataat
    16981 gtgtcaccac accagccata tttaatttct caaaaataag agatgaaatc tgattattta
    17041 gtgctccctt gccatcaata gtctccttac gagctccatt caacatagta gcttggtctt
    17101 tatagaccgt tctaatcaca ttttcatctt ttgtactata aatatctttc gcttttcctt
    17161 tataaataag ctggttagtc acttgattat tcgcctttct cttattttaa gagttattct
    17221 aacacacaac attatctaat acaacaaaaa ctttatttaa aatcaaaaaa aagaagaaac
    17281 gttcgtattt tcttcctttt tattattact tatgttcaat gatataatct aatagttgtt
    17341 ggacgcttga catgtgttcg acagcctcat ctggaatctc tagatgaaac acatcttcca
    17401 aattgataat aaactctgtt aaagcgatag agtcaattgc taaatcatca tgtaacgatg
    17461 actctggtgt gatatctaac tgtgggtcac caagttgctg ccttaataat tccagcatct
    17521 tttcaaaaac ttcatctctt gacatgagtt cactcctatt catctgtaaa ggcatcaact
    17581 aattgatcaa ctacttgagt ttccaacatt gtacgaactt gcttcaaagt agaataaaca
    17641 gctttagaat cacttgatcc atggcatttt actattggag cttttaaacc aaaaagaacc
    17701 gcacctcccg cgcttgaata gtccatacta tcttttagct gatacaggct atcttttaat
    17761 agaagtgctc ctaatttagc tttcacgcca cctgatttaa ttgaagattt tagacttccc
    17821 ataatattca tagctgtacc ttccattgtc tttagaacag catttccagt aaaaccatca
    17881 gtgacaacaa tatctgctac actagacatc aaatcacgtg cttcaatatt tccaataaaa
    17941 ttaatagaag gctctgctgc taacagttca taggcttctt tatgaagaga atcgcctttt
    18001 gtttcttccg ttccgttatt taagagacct acacgaggaa cttcaatacc acggacattt
    18061 tttgcataaa acgaccctaa aatagcatat tgatgaagat gagaagctgt gttttctgca
    18121 ttagctccta aatcaagcat atcaaaaccc acaccatcta acgtaggcat agttgacatt
    18181 aagcctggtc gatcaacacc ttttatacgt cctactacaa acaaaccagc agctaataaa
    18241 gctcctgtat tccctgcaga aataaaggcc tgtgcaacac catctttgac tgccttagct
    18301 cctaagacca tcgaagactg ttttttacga cgaacagctt tagcaggctc atcatcagaa
    18361 ttaatttttt cttcagtatg aataatacta actcgctccg ttactgttaa atatttctca
    18421 atttttttct gatcgccata gagttgtact tcaatatcac taaaatctga aatagcttga
    18481 ttaacaccct ctacaatagc tttgggagcg taatctccac ccatagcatc tacagcaatt
    18541 ttcttcatga atgaactcct cataaaaact tagtggctat attatagcat gtttctttat
    18601 ttttttcaca agaaggcaat aatagctaat atctaacaga tactttcact ctttccaaag
    18661 aaaagtatgc taaacaacct actaatatca aagaacatag tactccccct tcaaaaccaa
    18721 aatccccacc actccaaata gcaaagctag aattttcagt aattctaaaa agagaaacag
    18781 gagaataccc cccactaacc ttattaccta atacacatcc catagtaaaa ttccatgcag
    18841 tatgaactcc tgtaacaaaa agaatatttt tggtcatatt aaaaagaata gaaaaaataa
    18901 taccaagtaa aaataaatta aaaagtgcta ttgctgtgat accatagttt ctaaagtgga
    18961 taaaggaaaa caacatagaa ttgcataaaa cacctgcaaa actaccttta aatttggtaa
    19021 ctgttgtcat aacatatcct ctaaataaaa tttcctctgt caagctttgg ataaagtatg
    19081 caagtaataa aaagaaaaaa ggtccaaaag aaaaggaatt gctggaagaa atttccacaa
    19141 aacctgataa ccagtggcaa atataaataa aaaacatcag taaactacca gtaataaatc
    19201 ctgagatcgt tgttaaaaca ttattatctt tagtaaatcc caaggaagta aaatcccttt
    19261 tttgaaaata gattaaaaat agtaaaatac ttgccaaaat aggaataaat agtaaaagcg
    19321 caatgtataa attgaattta taatcatcag gtagcaattt tactacaagg cgtctactta
    19381 caatgatgcc gataacttga attagaactg caccaataac tggcacaaac cagttagcaa
    19441 aaaagagttt attggtaatg tataatgttt ttttcatctc aaatatccta aaaattaatt
    19501 ccatattagt ttatcctaat attaaaaaaa agaacatgac actaatgtca tgttcctctt
    19561 tacaacttaa tctgatttca taatacttcc ccaactagac aaatcatcaa taaatttttt
    19621 acttttaaga tgaatcccta catattcatc gtaaatcatg tccaaaaatt gacgaatttt
    19681 aagtttcatc tcaggtttca cagaaattgt ttgtaaatca tcaaattgaa ttgactgaaa
    19741 acgattgatt aagtacagca tattaggatc taggtggttt cttctctcgt ctttataata
    19801 atggtttgga cataataatc cagaatattt gtgtgaaaaa tcaaaaggta atcctgccct
    19861 atgacaaaaa acacaatcat gaaaatttaa agcaacaccg aacctctcta gtagttgtac
    19921 ttcaaaaata ttcgttagaa tctcataatc taaaccgtcc tcaatcaatt ctaacgtttt
    19981 ctttaggaaa ataaataatt gagcatccgc tacattatca ctaatagcca catcagccaa
    20041 agaagtaata taactcgcat atgacaactt aaacaagtct gaattggttt cttgaaatgc
    20101 taaaacttct ttataatcat caatataaga aagaccatta tcattaattt ttaaaataaa
    20161 atgagcaata gtcaaaggtt ggagcacagc attgaatttt gactttgaag catgctttac
    20221 aaaaaacatt cttttcccct cagtctccgt aaagatttta actaatttat cgtcttcacg
    20281 ataattacga ttatacaaaa cgagaccgta tgtttgacta accctcatat gattccataa
    20341 aaactttcaa tctagccatt gctgtttcaa taacttccat actagcagca taagaaagac
    20401 gtatataccc ttcaccatat ttaccaaaag ccacccctgg aataatagct acagcttgtt
    20461 gataagcaaa atcctgacaa aacttgaaag aatcctgtgg ataactatca ggtattttag
    20521 caaagatata aaaagcgccg ctcggtttaa ttattttgaa accaagttta gacattttat
    20581 caatgatata atctctacgt ttaatatatc cttgacgcat aggtaacgta tcatttttac
    20641 ccacagataa ggcttctacc ccagcaaatt gcgaaatagt actagcagct gtcaccatgt
    20701 attgatgact tttaatgatt tgagcagtaa atgcttcagg agcatacacc aatccaactc
    20761 tccaaccagt catagcatgt gattttgata agccattaat taagatagtt tggttaggta
    20821 aatattctgc aattgaaaca tgttgctgac cagtgtaagt taactcagag taaacttcat
    20881 cactaataac aaaaatatca tattttttaa ggacttcagc taatgcagca atttcttgcc
    20941 ttgaataggt aatacctgta ggattagtag gataattaag taaaacagcc tttaattttt
    21001 caccttgttg gataatagcc gtttctaaca tttcaggggt tagtctaaag tcattttctc
    21061 tagtatcaat ttctacgata tcagctccta caaggttaac tattggctca tagcctggat
    21121 aagcgggggc aggtaataga acaacatcac cagcttctaa aatagctata agagatgcag
    21181 acagagcttc tgtggcacct atcgtaacca agatctcact atcagggtta taagtaagat
    21241 tatatttatc ctttgcaaaa tctgcagcag cttgccttag agctaataaa ccgctcatac
    21301 cagtataata cgattgattc gcatcaatag ctgatttagc tgcttcttta acatgatcag
    21361 gagtggtaaa atctggttct ccaagagtta attttaccat tccaggtata tctgaaatag
    21421 attgatcaaa ctgacgaatt aaggagacct ctattctatc taagtactta ttaaatcgtt
    21481 tctctaatgt catcataacc tcctgataag aaataatata tttaattata caagaaaaat
    21541 aatttgtgtc cagttttccg tcattaaatg ataaaaaacc tgttaataac aggtttttta
    21601 tcattttagt ttattcattt caaataatgg tgataaagga cgtttctcgt gaatacggat
    21661 aatagcttca ccaatcaatt ccgcaattga aatttgttca atcttatcaa tcaaacgttc
    21721 ttcagggagg taaatcgtat ctaacacaat taatttttca atagctgaat tttgaatatt
    21781 atccaaagcc ggccctgata aaacagggtg agtacatgaa gcataaacag ctgttgcccc
    21841 tgcttcagct aaagcgtctg cagcatggca aatagtacct gc
//
LOCUS       DATRQA010000017        21823 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.017, whole genome shotgun sequence.
ACCESSION   DATRQA010000017 DATRQA010000000
VERSION     DATRQA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21823
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.017"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(125..691)
                     /locus_tag="VBD27_000481"
     CDS             complement(125..691)
                     /locus_tag="VBD27_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000596040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7024516.1"
                     /translation="MIKRPIHLSHDFLAEVIDKEAITLDATMGNGNDTVFLAKSSKKV
                     YAFDIQEEAIAKTKAKLTEQGISNAELILDGHENLEQYVHTPLRAAIFNLGYLPSADK
                     TVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSERDAVIAFVEQLPQNNFAT
                     MLYQPLNQVNTPPFLIMVEKLQSYENEV"
     gene            complement(684..1619)
                     /locus_tag="VBD27_000482"
     CDS             complement(684..1619)
                     /locus_tag="VBD27_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO7024517.1"
                     /translation="MKKRYRAINDYYRELFGEKIFKLPIDAGFDCPNRDGTVARGGCT
                     FCTVSGSGDAIVAPEAPIREQFYKEIDFMHRKWPEVNKYLVYFQNFTNTHAKLEIIKE
                     RYEQAINEPGVIGINIGTRPDCLPDETIYYLAELSERMHVTLELGLQTTYEATSALIN
                     RAHSYDLYKKTVKRVRELAPKVEIVSHLINGLPGETHDMMVENVRRCVTDNDIQGIKL
                     HLLHLMINTRMQRDYHEGRLRLLSQEDYISIICDQLEIIPKHIVIHRITGDAPRHMLI
                     GPMWSLNKWEVLNAIDKEMEKRQSYQGCKVEVKHD"
     gene            complement(1712..2353)
                     /locus_tag="VBD27_000483"
     CDS             complement(1712..2353)
                     /locus_tag="VBD27_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7024518.1"
                     /translation="MLSRQNSKLIQAFIAIILFFSLGLVIKYWPDTVIAFDQTIQESV
                     RGQLPNLSTRFFKLITVVGNTVSQIAIAIMSVTFCYLKKWYPQARFIAVNAIISGICI
                     LSLKLIFQRVRPTLTHLVFAGGYSFPSGHSMGTFMIFGSSIILLQYYMPKSIWKLLCQ
                     GTLGLLIFLIGLSRIYLGVHFPTDVLAGFILAYGILNLTAYIFLSKDWLKKVK"
     gene            complement(2334..2918)
                     /locus_tag="VBD27_000484"
     CDS             complement(2334..2918)
                     /locus_tag="VBD27_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7024519.1"
                     /translation="MTNTRKMVTIAILSALSFVLMMVSFPLIPGAEFLKVDFSILPML
                     VAFILFDLKSSYGVLLLRSLLKVILANRGPETFIGLPMNMVALALFLASFAIFWKNRE
                     SAKDFIKASLFGTVSLTVSMVALNYVFAIPLYAIFANFDIRTFIGVGNYLLTMVIPFN
                     IVEGILISIVFYLTYVACLPILERYKKTNVISSK"
     regulatory      complement(2988..3133)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3202..3801)
                     /locus_tag="VBD27_000485"
     CDS             complement(3202..3801)
                     /locus_tag="VBD27_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEO7024520.1"
                     /translation="MIRRYSANFLAILGIILVSSGIYWGWYNINQAHQADLTSQHIVK
                     VLDKSITHQVKGSENGELPVKKLDKTDYLGTLDIPNLKLHLPVAANYSFEQLSKTPTR
                     YYGSYLTNNMVICAHNFPYHFDALKNVDMGTDVYFTTTTGQIYHYKISNREIIEPTAI
                     EKVYKTATSDNDWDLSLFTCTKAGVARVLVRCQLIDVKN"
     gene            complement(3794..4465)
                     /locus_tag="VBD27_000486"
     CDS             complement(3794..4465)
                     /locus_tag="VBD27_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024521.1"
                     /translation="MIYKKILKITLLLLFSLSTQLVSADTNDQMKTGSITIQNKYNNQ
                     GIAGGNLLVYQVAQAKDVDGNQVFTLTTPFQGIGIKDDDLTQVNLDSNQAKYVNLLTK
                     AVHKTQPLQTFDNLPAEGIVANNLPQGIYLFIQTKTAQGYELMSPFILSIPKDGKYDI
                     TAFEKMSPLNAKPKKEETITPTVTHQTKGKLPFTGQVWWPIPILIMSGLLCLIIALKW
                     RRRRD"
     gene            complement(4455..5330)
                     /locus_tag="VBD27_000487"
     CDS             complement(4455..5330)
                     /locus_tag="VBD27_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO7024522.1"
                     /translation="MKKIRQHLVSILLVTIIAVGVGLMAYPSLANYWNSFHQSRAIMD
                     YQDRVTHMDENDYKKIINRAKEYNKQFKTSGMKWHMTSQERLDYNSQLAIDKTGNMGY
                     ISIPKINIKLPLYHGTSEKVLQTSIGHLEGSSLPIGGDSTHSILSGHRGLPSSRLFSD
                     LDKLKVGDHWTVSILNETYTYQVDQIRTVKPDDLRDLQIVKGKDYQTLVTCTPYGVNT
                     HRLLVRGHRVPNDNGNALVVAEAIQIEPIYIAPFIAIFLTLILLLISLEVTRRARQRK
                     KILKQAMRKEENNDL"
     gene            complement(5368..6876)
                     /locus_tag="VBD27_000488"
     CDS             complement(5368..6876)
                     /locus_tag="VBD27_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO7024523.1"
                     /translation="MKKKMIQSLLVASLAFGMAVSPVTPIAFAAETGTITVQDTQKGA
                     TYKAYKVFDAEIDNANVSDSNKDGASYLIPQGKEAEYKASTDFNSLFTTTTNGGRTYV
                     TKKDTASANEIATWAKSISANTTPVSTVTESNNDGTEVINVSQYGYYYVSSTVNNGAV
                     IMVTSVTPNATIHEKNTDATWGDGGGKTVDQKTYSVGDTVKYTITYKNAVNYHGTEKV
                     YQYVIKDTMPSASVVDLNEGSYEVTITDGSGNITTLTQGSEKATGKYNLLEENNNFTI
                     TIPWAATNTPTGNTQNGANDDFFYKGINTITVTYTGVLKSGAKPGSADLPENTNIATI
                     NPNTSNDDPGQKVTVRDGQITIKKIDGSTKASLQGAIFVLKNATGQFLNFNDTNNVEW
                     GTEANATEYTTGADGIITITGLKEGTYYLVEKKAPLGYNLLDNSQKVILGDGATDTTN
                     SDNLLVNPTVENNKGTELPSTGGIGTTIFYIIGAILVIGAGIVLVARRRLRS"
     gene            complement(6917..11221)
                     /locus_tag="VBD27_000489"
     CDS             complement(6917..11221)
                     /locus_tag="VBD27_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HEO7024524.1"
                     /translation="MLKKCQTFIIESLKKKKHPKEWKIIMWSLMILTTFLTTYFLILP
                     AITVEETKTDDVGITLENKNSSQVTSSTSSSQSSVEQSKPQTPASSVTETSSSEEAAY
                     REEPLMFRGADYTVTVTLTKEAKIPKNADLKVTELKDNSATFKDYKKKALTEVAKQDS
                     EIKNFKLYDITIESNGKEAEPQAPVKVEVNYDKPLEASDENLKVVHFKDDGQTEVLKS
                     KDTAETKNTSSDVAFKTDSFSIYAIVQEDNTEVPRLTYHFQNNDGTDYDFLTASGMQV
                     HHQIIKDGESLGEVGIPTIKAGEHFNGWYTYDPTTGKYGDPVKFGEPITVTETKEICV
                     RPFMSKVATVTLYDDSAGKSILERYQVPLDSSGNGTADLSSFKVSPPTSTLLFVGWSK
                     TQNGAPLSESEIQALPVSSDISLYPVFKESYGVEFNTGDLSTGVTYIAPRRVLTGQPA
                     STIKPNDPTRPGYTFAGWYTAASGGAAFDFNQVLTKDTTLYAHWSPAQTTYTINYWQQ
                     SATDNKNATDAQKTYEYAGQVTRSGLSLSNQTLTQQDINDKLPTGFKVNNTRTETSVM
                     IKDDGSSVVNVYYDRKLITIKFAKYGGYSLPEYYYSYNWSSDADTYTGLYGTTLAANG
                     YQWKTGAWGYLANVGNNQVGTYGMSYLGEFILPNDTVDSDVIKLFPKGNIVQTYRFFK
                     QGLDGTYSLADTGGGAGADEFTFTEKYLGFNVKYYQRLYPDNYLFDQYASQTSAGVKV
                     PISDEYYDRYGAYHKDYLNLVVWYERNSYKIKYLDPLDNTELPNFPVKDVLYEQNLSS
                     YAPDTTTVQPKPSRPGYVWDGKWYKDQAQTQVFDFNTTMPPHDVKVYAGWQKVTYRVN
                     IDPNGGRLSKTDDTYLDLHYGDRIPDYTDITRDYIQDPSGTYYYKYDSRDKDPDSTKD
                     AYYTTDTSLSNVDTTTKYKYVKDAYKLVGWYYVNPDGSIRPYNFSGAVTQDINLRAIW
                     RKAGDYHIIYSNDAVGTDGKPALDASGQQLQTSNEPTDPDSYDDGSHSALLRRPTMPD
                     GYRFRGWWYNGKIYNPYDSIDIDAHLADANKNITIKPVIIPVGDIKLEDTSIKYNGNG
                     GTRVENGNVVTQVETPRMELNSTTTIPENQYFTRTGYNLIGWHHDKDLADTGRVEFTA
                     GQSIGIDNNPDATNTLYAVWQPKEYTVRVSKTVVGLDEDKTKDFLFNPSETLQQENFP
                     LRDGQTKEFKVPYGTSISIDEQAYDEFKVSESITEKNLATGEADKTYDATGLQSLTVS
                     GDVDISFTNTRIKQKVRLQKVNVENDNNFLAGAVFDIYESDANGNKASHPMYSGLVTN
                     DKGLLLVDANNYLSLPVGKYYLTETKAPPGYLLPKNDMSVLVISTGVTFEQNGNNATP
                     IKENLVDGSTVYTFKITNSKGTELPSTGGIGTHIYILVGLALALPSGLILYYRKKI"
     gene            complement(11241..11810)
                     /gene="lepB"
                     /locus_tag="VBD27_000490"
     CDS             complement(11241..11810)
                     /gene="lepB"
                     /locus_tag="VBD27_000490"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO7024525.1"
                     /translation="MKRQISSDKLSQELDRVTYQKRFWSVIKNTIYILMAVASIAILI
                     AVLWLPVLRIYGHSMNKTLSAGDVVFTVKGSNFKTGDVVAFYYNNKVLVKRVIAESGD
                     WVNIDSQGDVYVNQHKLKEPYVIHKALGNSNIKYPYQVPDKKIFVLGDNRKTSIDSRS
                     TSVGDVSEEQIVGKISFRIWPLGKISSIN"
     gene            complement(11794..12066)
                     /locus_tag="VBD27_000491"
     CDS             complement(11794..12066)
                     /locus_tag="VBD27_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024526.1"
                     /translation="MEDLKKLKRKDLLEIMLEQQLLIEKQEKEIQQLKEALDDKKIKI
                     SKAGSIAEASLMLSGIFETAQLAADTYLENVKNLVDKRGVDNEKTD"
     gene            complement(12348..13496)
                     /locus_tag="VBD27_000492"
     CDS             complement(12348..13496)
                     /locus_tag="VBD27_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO7024527.1"
                     /translation="MENKVKTVAVFSGYYLPFLGGIERYTDKMTADLVKRGYRVVIVT
                     TNHDDLPIIDEDKGRKIYRLPTKNIVKQRYPIINKNREYNTLMKHVSDENIDFVICNT
                     RFQLTTLEGLSFAKKHRLPSIVLDHGSSHFSVNNRFLDFFGAIYEHLLTARVKHYRPD
                     FYAVSKRSVEWLKHFNIEAKGVIYNSVSESLGSDFADTAYLEKSADDIFITYAGRIIK
                     EKGIELLLEAFSMSQYSENVYLQIAGDGPELAHLKEKYQSKQINFLGKLNFEQTMSLM
                     AQTDIFVYPSMYPEGLPTSILEAGLLSSAIIATDRGGTVEVIDSPELGIIMEENTQSL
                     HESLDLLVKDKALREKLQQNIAKRIKEHFTWEKTVEKLDYIIQKELNL"
     gene            complement(13486..14334)
                     /locus_tag="VBD27_000493"
     CDS             complement(13486..14334)
                     /locus_tag="VBD27_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7024528.1"
                     /translation="MKYLAGIVTFNPNIERLDQNIRAIYPQVSHIYIVDNGSKNKEEI
                     SQLVADYNEEGHLTVDYLTENKGIAYALNCIGQFAVAQEFDWFLTLDQDSVVLGDLID
                     NYENYLLLPKVGMLSCLYQDMNREDLVMQEFDYKEIEECITSAALMKTSVFEETSGFA
                     EEMFIDFVDSEMNYRLSEMGYKTYQVNFIGLLHEIGHSSRVKKFGHVFHVLNHSPFRK
                     YYMIRNAIYIIKKYGKKKRHKYLVFMRNEFVRVLVAEEQKSKKLVAMIKGLKDGLLMK
                     VDKYGK"
     gene            complement(14331..15755)
                     /locus_tag="VBD27_000494"
     CDS             complement(14331..15755)
                     /locus_tag="VBD27_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO7024529.1"
                     /translation="MKLLKNMFYNTSYQLLTLLLPLVTVPYVSRVLSPQGIGINAYTS
                     SIVMYFTLFGALGISLYGNREIAFVQSNKYKRSKIFWELVVLKLASVSIATLLFFGFV
                     LLTNEWQLFYLIQGINLLATATDISWYFIGVEDFKIIVIRNTIVKLITVVLTFLVVKT
                     PDDLALYMFLIAFASLLGNLTVWHHLKHEIIKIPFSRLDILIHLRPTLMLFLPQITMQ
                     IYLSLNKSMLGAMDSVVSAGYFDQSDKIIRILFTIVSAIGGVFLPRLSSLFSSGKEKQ
                     AKALLLKLVDLSNAISMLMIAGVVGVSSTFAVFFFGKGYEAVGPLMAVESLMIICISY
                     GNALGTQYLLASRRTKAYTMSAVIGLVANVVLNILLIPILGAMGAIISTVITEFIVSL
                     YQAISLRDVFTFKELTRGMLRYLIAATLSGAVLYYINTQMSVSLVNYVIQSLVAVTIY
                     VGIVFITKAPVIQLFRDFRKEHRT"
     gene            complement(15755..17119)
                     /locus_tag="VBD27_000495"
     CDS             complement(15755..17119)
                     /locus_tag="VBD27_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEO7024530.1"
                     /translation="MNLKKVRLFEKFFLDKNLHKTYFILGLTITIVLSLLMPFFNEPD
                     GQYHLAVSGRISNNIIDTSYYGEYNIDSGMRRQSTSYRDGTRFEKYYLNKANFVDGNH
                     LPREINYSKHNFVFWGHLVPAVGLFLGKFVYPSMGVMITVARLFSSFVSITLVALIIR
                     YVKKGKLIYFAIFLSPVVLNSFASLSYDSTGYIVFGAFLSLLINLLVDKSLTKNRSIA
                     LIGLILLVIVACKQNYWLFLLLVIPVLLQVNNPKLMGYNQKLSHLFQGLKSKKLFWLC
                     LTFLSLAIVYVLTARHGGVLAVLRRYVMTFGYNYAGINILSNDITSWLASPYPSQNFI
                     PTWVSSVWYLMVFLVWFSEEKFVKSKNMGFLFLASFFVGILGVFFIMLEYRGAPTSYI
                     EGVQGRYFTVTLLLVQVFASSIPARLNEAGRKVVPYALTGLVIVSNALLLFDTVISLI
                     MRRN"
     gene            complement(17121..18068)
                     /locus_tag="VBD27_000496"
     CDS             complement(17121..18068)
                     /locus_tag="VBD27_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7024531.1"
                     /translation="MVKTAVLMATYNGQGFIHDQLDSIRNQTLRPDYVLMRDDGSTDD
                     TVKVVEDYIKEHRLDGWSITSNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDTWYH
                     HKNKMQVDIMEERQDINLLSADIDIKKLSEEATIPNNFKFNGEETISKYPLDFSYHNY
                     RQGWTFCLRKSFVDQVMTFYSDNLILSHDNLFAGVSGVIGSGYNLNRSVGEHKRHAEN
                     ASGNILNMSSSYERHLQELYFVLSYYIILVPFLEKSSSSQLREAKAYLDFNQQRYKNA
                     QNKDFVAILKQMMTKKRFYDSFSNRIRDLVFLLKDIKRS"
     gene            complement(18077..19012)
                     /locus_tag="VBD27_000497"
     CDS             complement(18077..19012)
                     /locus_tag="VBD27_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7024532.1"
                     /translation="MVKTAVLMATYNGEKFISEQLDSIRQQTLKPDYVLLRDDCSTDE
                     TVNVVNNYIAKHELEGWKIVKNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDIWYL
                     DKNERQFAIMSDNPQIEVLSADVDIKTMSTEASVPHFLTFSSSDRISQYPKVYDYQTF
                     RPGWTIAMKRDFAQAIADNWTFGANISHDNLIETTASIIGNGYNLNESVGVHIRYGGN
                     ASGNSKFVLNWKSPKTQHITGLKTFVDFYETMQKVVNKYQNDVALNQRLAFEKERLEA
                     SKLSLIHNSAFVIKNWRLYNSNSGRMRDLLFAFKK"
     gene            complement(19014..20072)
                     /locus_tag="VBD27_000498"
     CDS             complement(19014..20072)
                     /locus_tag="VBD27_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO7024533.1"
                     /translation="MMIKFSNKIVQEDLALISNQNYINWERIANKGVLISGSNSMLAS
                     YMVFLLAYLNETRNYQTQIIATARNIEKARDKFSDLVGKDYFTLIPYDVEERLEYDGK
                     VDYIIHAASNASPTAILSNPVSIIKANTIGTLNLLDFAKEKTIENFLFLSTREVYGTS
                     IKEVIDEEAYGGFDILATRACYPESKRMAETLLQSYYDQYKVPFTIARIAHSFGPGME
                     LGNDGRIMNDLLSNVIDGKDIVLKSSGTAERAFCYLADAVSGLFTILLNGEVGQAYNV
                     ANEDQPIMIKDLAQKLVDLFSDKNISVVFDIPKTMSAGYSKMGRTRLTMAKLEALGWK
                     REVSLESGILKTVQAFEE"
     gene            complement(20072..20794)
                     /locus_tag="VBD27_000499"
     CDS             complement(20072..20794)
                     /locus_tag="VBD27_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEO7024534.1"
                     /translation="MMNIGVIFAGGVGRRMNTKGKPKQFLEVHGKPIIVHTIDIFQNT
                     EAIDAVVVVCVSDWLDYMNNLVERFNLTKVKAVVAGGETGQMSIFKGLEAAEQLATDD
                     AVVLIHDGVRPLINEEVINANIQSVKETGSAVTSVRAKETVVLVNDSSKISEVVDRTR
                     SFIAKAPQSFYLSDILSVERDAISKGITDAIDSSTLMGMYNRELTIVEGPYENIKITT
                     PDDFYMFKALYDARENEQIYGM"
     gene            complement(20794..21618)
                     /locus_tag="VBD27_000500"
     CDS             complement(20794..21618)
                     /locus_tag="VBD27_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEO7024535.1"
                     /translation="MKEMTVSEIREVQLEMLAYIDKVARDNKIEYSLGGGSLLGAMRH
                     KGFIPWDDDIDLMLERSQYERLMKALADANNSDFKLLHHSVEKNLWPFAKLYHTKSMY
                     LSKTDRIHPWTGIFIDIFPLDRLPESAEERQRFFKKVHSAAANLMCTTYPNFASGSRK
                     LYANARLILGLPRFIAYHGQAKKRAEIVDQVMETYNNQEVPYMGYTDSRYRLKEYFPR
                     EIFSEYEDVMFENIKTRKIKNEHAYLNQLYGGSYMELPPESKRESHSYYTWYWKEG"
     gene            complement(21643..>21823)
                     /locus_tag="VBD27_000501"
     CDS             complement(21643..>21823)
                     /locus_tag="VBD27_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="surface protein"
                     /protein_id="HEO7024536.1"
                     /translation="INGYIMDMQYNKQEYNALANTPKGQKTKKTFKPMTLVILFAVAL
                     FIFYITAIRFVFKRM"
BASE COUNT         6952 a         4179 c         3367 g         7325 t
ORIGIN      
        1 acggattttc gttggttttt attggttttt atcagactaa attccatttc tactagaggt
       61 ggaacttact ttgagaattt agcttttata gcaattatca tttttgctaa ttgcctaaag
      121 atagttacac ctcattttca tatgattgta acttttctac cataattaaa aaggggggcg
      181 tattaacttg attgagtggt tgataaagca ttgtagcgaa attattctgt ggtaattgtt
      241 ccacgaatgc tatcaccgca tctctctcac tttttccacc atcatgacca taataaacca
      301 taagtgatag acgtccacct acttctaaaa tgtctaaaac gtttttgatt gccttgatag
      361 ttgtgtgtgg ttttgtaata actgttttat ccgctgaggg taagtatccc agattaaata
      421 tagccgctct taaaggtgta tgaacatact gttccaaatt ttcatgccca tccaagataa
      481 gttcagcgtt agagatccct tgctccgtga gctttgcttt cgtttttgct attgcttctt
      541 cttgaatatc aaatgcataa acttttttag aacttttcgc taaaaaaaca gtatcattcc
      601 catttcccat agttgcatca agcgttattg cttctttatc aataacttct gctaaaaaat
      661 catgagaaag atgaattggt cttttaatca tgttttacct caactttaca tccttgatat
      721 gactgtcttt tctccatttc tttgtcaatg gcattaagca cttcccattt attaaggctc
      781 cacatcggtc caattaacat atgacgtggc gcatcaccag taattcggtg aataacaata
      841 tgttttggaa taatctccag ttggtcacaa ataatcgaaa tatagtcttc ttgacttaac
      901 aaacgtaaac gaccttcatg gtaatctctc tgcattcttg tattaatcat aagatgcaga
      961 aggtgtaatt ttataccctg aatatcatta tccgtaacac atcgacgaac attttccacc
     1021 atcatatcat gtgtctcccc tgggagacca tttattaggt gagaaacgat ttctacttta
     1081 ggagctaatt ctcgtactct cttaacagtt tttttgtaaa ggtcatatga atgtgctcta
     1141 tttattaatg cagaagttgc ttcataagtt gtctgaaggc ctaattctaa agtcacatgc
     1201 attctttcag aaagttcagc gagatagtat atagtttcat caggtaagca atccggcctt
     1261 gttccgatgt tgatcccgat aactcctggc tcattaatag cctgttcgta acgctcttta
     1321 attatctcta acttagcatg ggtattggta aaattttgaa aatagactaa gtatttatta
     1381 acctcaggcc actttctatg catgaaatca atttctttat agaattgttc acgaatagga
     1441 gcttctggag caactatagc atcccctgaa ccagaaactg tgcaaaaagt gcaccctcct
     1501 ctagcaactg ttccatctct gttaggacag tcaaaaccag catctatagg taatttaaat
     1561 attttttctc caaagagttc tcgataataa tcattaatcg cacgataacg ttttttcata
     1621 ggataattgt atcacaattt taactaaaat aacctcacta ctacaataaa actaaaaaag
     1681 attggaacgt cagttagtcc caatctttta tttacttcac tttctttaac caatccttgg
     1741 ataaaaagat atacgcagtt agattcaaaa taccataagc aagtataaaa ccagctaaaa
     1801 catctgtcgg aaaatgaacc cctaggtaaa tacgagataa cccaattaaa aaaatgagca
     1861 aacccaatgt accttggcac aacagtttcc atatactctt aggcatatag tactgcaata
     1921 aaataatact actcccaaat atcataaatg ttcccatcga gtgcccactg ggaaacgaat
     1981 agccacctgc aaatactaaa tgggttaaag ttggtctcac tctttgaaaa ataagtttta
     2041 aagaaagtat acatatacca gagataatag catttactgc gataaatcta gcttgaggat
     2101 accacttctt aaggtaacag aaagtgacgc tcataatcgc aatagctatc tggcttacag
     2161 tattaccaac cacagtgatt aacttaaaaa atcttgtaga aagatttggc aactgtcctc
     2221 taacactttc ttgaatagtt tggtcaaatg cgattacagt gtcgggccaa tatttgatga
     2281 ccaatcctaa actgaaaaat aagataatag caataaatgc ttgaataagt ttactatttt
     2341 gacgagataa cattagtctt tttatatctt tctaatattg gcaaacaagc cacgtaagtt
     2401 agatagaaaa caatcgaaat taaaattccc tcaacgatat taaatggaat aaccattgtt
     2461 aaaaggtaat tgcctacacc aataaatgtt ctgatatcaa agttagcaaa tatagcatac
     2521 aaaggaatcg caaagacata gttgagagct accatagata cagtcaagct aactgtacca
     2581 aataaactag ctttaataaa atcttttgca ctctctctat ttttccagaa aatagcgaaa
     2641 cttgctaaaa atagagctag agcaaccata ttcatcggta aaccgataaa ggtttctgga
     2701 ccacgattag caagtataac ttttaaaagt gatcttaata agagtacacc ataacttgat
     2761 ttcaaatcaa ataaaataaa agcaactaac atcggaagga ttgaaaaatc aacctttaaa
     2821 aattctgctc ctggtattaa tggaaatgaa accatcatca atacaaaaga taaggcagaa
     2881 agaatggcga ttgtcaccat tttacgtgta tttgtcataa aaaaattcct ccaatttaaa
     2941 taaattgaaa gaagctccaa aggtaagcgt aggtacgcga aaaaaacctt tgtcttctcc
     3001 catccagact ttactgtcgg ttgtggaatc tcaccacatc agctttcgct cgcggactga
     3061 tgcttcacaa ctgacaaata agttggaagc gattaccgcc ggtcgggaat tacaccctgc
     3121 cctgaagaca cctatagcat aacaaaaaaa acttgcaatt gcaagttttt taatcactaa
     3181 ttagtagtag attgtataat attaattttt aacatcaatt aattgacagc gcactaatac
     3241 tctagctact cctgcctttg tacaagtaaa caagcttaag tcccaatcat tgtctgatgt
     3301 ggcagtttta taaacttttt caatcgctgt tggttcaata atttctctat tactgatttt
     3361 gtagtgatag atttgccctg ttgtagttgt aaaataaaca tccgttccca tatctacatt
     3421 ttttaaagca tcaaaatgat aaggaaaatt atgcgcacaa atcaccatgt tattagttaa
     3481 ataagaacca taataccttg taggcgtctt agacagttgt tcaaaactat aattagcagc
     3541 taccggtaaa tgcagtttta agttcggaat atccagagtt cccaagtaat ctgttttatc
     3601 caactttttt acaggtaatt ctccattttc tgaacccttt acttgatgcg taatagattt
     3661 atcaagcacc ttgacaatat gctgagaagt taaatcagct tgatgcgcct gattaatatt
     3721 ataccaaccc caatagattc cagaacttac cagaataatt ccgagtatag ctaaaaaatt
     3781 tgctgaatat cttctaatca cgtcttcttc tccattttaa agctattatt aaacacagaa
     3841 gtcctgacat aattagtata ggtattggcc accatacttg tccagtaaac ggaagctttc
     3901 cctttgtctg atgtgttact gtaggagtaa ttgtctcttc ttttttaggt ttagcattta
     3961 aagggctcat tttctcaaat gctgtaatat cgtacttccc atccttaggt attgatagta
     4021 taaagggaga cattagttca taaccttgag ccgttttagt ctgaataaat agataaatcc
     4081 cttgaggaag attgttcgca acaatacctt cagccggtaa attatcaaac gtttgtaaag
     4141 gttgagtttt atgaacagct tttgttagta gattgacgta tttggcttgg ttactatcaa
     4201 ggtttacttg tgttagatca tcgtctttta ttccaatacc ttgaaatggg gtagttagag
     4261 taaaaacttg attaccatca acatctttag cttgtgctac ttggtaaaca agtaaattac
     4321 cgccagcgat accttgatta ttatacttat tttgtatagt aatagaaccc gttttcatct
     4381 gatcattggt atcagcagac acaagttgag tacttagact aaataataag agaagagtta
     4441 tctttaggat ctttttataa atcattgttc tcttcctttc tcattgcttg ttttaaaatt
     4501 ttcttacgtt gacgtgctct cctagttact tctaaagaga ttaaaagtaa aatcaaagta
     4561 aggaaaatag cgataaatgg tgcgatataa ataggctcta tttgtattgc ctctgctact
     4621 accaaagcgt taccattatc gtttggtaca cgatgtcctc tcactagtaa ccgatgggta
     4681 ttaacgccat atggtgtaca cgtcaccaaa gtttggtagt ctttaccttt aacaatttgt
     4741 aaatccctca aatcatccgg tttaactgtt ctgatttgat ccacttgata agtatatgtt
     4801 tcatttaaga tactgactgt ccagtggtct ccaactttta acttatccaa atcagaaaaa
     4861 agccttgaag agggtaaacc tctatgtcct gataaaatag aatgagttga gtctcctcca
     4921 attggaagac tacttccttc taaatgacca atagaagttt gaagcacttt ttcacttgta
     4981 ccatgataaa gtggtaattt tatgtttatc tttggaattg aaatataacc catattaccc
     5041 gttttatcga tagccagttg tgaattataa tccaaacgct cttggctagt catgtgccac
     5101 ttcattcctg aagttttaaa ttgcttatta tattctttgg ctcggttaat aattttttta
     5161 taatcgtttt catccatatg cgttacgcgg tcttggtaat ccataatcgc tcgagattgg
     5221 tgaaatgaat tccaataatt agcaagtgaa ggataagcca ttaagcctac ccccactgca
     5281 attatagtga caagcaaaat ggatactaaa tgttgtctta tttttttcat atattttaaa
     5341 tctgtaccac tttgctagcc catcttatta agaacgtaaa cgacgacgag caacaagcac
     5401 gatacctgct cctattacta aaattgcacc tataatgtag aaaattgttg taccaatacc
     5461 acctgttgaa ggcaactcag tacctttgtt attttcaaca gttgggttaa ctaaaaggtt
     5521 atctgaatta gtcgtatcag tggctccatc tcctaaaata accttctgag agttatctaa
     5581 caaattgtaa cctaagggag cctttttctc aactagatag tatgtacctt ctttcaagcc
     5641 tgtaatggta attataccat ctgctcctgt tgtatattct gttgcattag cttctgtgcc
     5701 ccattcaacg ttatttgtat cgttaaagtt tagaaattga cccgtagcat tctttaaaac
     5761 aaatatagca ccttgtaatg aagcttttgt ggaaccatca atttttttta tagtaatttg
     5821 accatccctc actgttactt tttgacctgg gtcatcattg ctagtattgg ggttgatggt
     5881 cgcaatgttt gtattttctg gtaaatcagc tgaacctggt ttagctccac tctttaatac
     5941 tcctgtataa gtgactgtga ttgtatttat tcccttataa aaaaagtcat cattagctcc
     6001 attttgagta tttccggttg gagtattggt agctgcccac ggaatagtaa tcgtgaaatt
     6061 attattttcc tctaacaggt tatacttccc agttgctttt tccgaacctt gagttagagt
     6121 tgtaatattc cctgatccat cagtaatagt tacttcataa gacccttcgt tcaaatcaac
     6181 tacagaagca gatggcatag tatcctttat aacatattga tacacttttt ctgtaccatg
     6241 ataattgact gcattcttat aagtaatagt atatttgact gtatcaccaa ccgagtacgt
     6301 tttttgatct acagtttttc caccaccatc tccccatgtc gcatcagtat tcttttcatg
     6361 aatagtagca tttggagtta cagatgtaac cataattaca gctccattat taacagtgct
     6421 agaaacataa taatatccat attgggaaac attaataacc tcagtaccat cattatttga
     6481 ctcagtaaca gtggaaactg gtgtagtatt agctgatata gatttagccc atgtcgcaat
     6541 ctcatttgct gacgcagtat cttttttagt tacatatgtt ctccctccat tagtagttgt
     6601 cgtaaaaaga gaattaaaat cagttgaagc tttatactca gcttctttac cttgaggaat
     6661 taaataagaa gctccatctt tattcgaatc agatacattt gcattatcta tttctgcatc
     6721 aaaaacttta tatgctttat aggttgcgcc tttttgagta tcttgaactg taattgtccc
     6781 tgtctcagcg gcaaaagcta tcggcgtaac tggtgataca gccataccaa atgctaaact
     6841 cgccactaac agcgattgaa tcattttctt tttcattgaa atctttctcc taaaatcata
     6901 ttgatgaatg attaattcat attttttttc gatagtataa tattaatcct gatggtagag
     6961 ctaaagctaa accaactagg atataaatgt gtgttccaat acctccagta ctaggcaatt
     7021 ctgttccttt actgttagta attttaaaag tatatactgt acttccatcc actaaattct
     7081 cttttattgg tgtcgcatta ttaccatttt gttcaaaggt aactcccgta gaaatcacta
     7141 atactgacat atcattttta ggtagtaggt accctggagg ggcctttgtc tctgttaggt
     7201 agtattttcc tactggcaaa ctgaggtagt tattagcatc cactaataac aagcctttat
     7261 cgtttgtcac cagccctgaa tacataggat gtgaagcttt attcccatta gcatctgatt
     7321 cataaatatc aaaaactgca cctgctaaaa aattattatc attttcgaca ttaactttct
     7381 gtagtcgtac tttttgcttg atacgtgtat tggtaaagct aatatctacg tctcctgaaa
     7441 ctgtcaggga ttgtaagccg gtagcatcat aagttttatc agcttcacca gttgctagat
     7501 ttttttctgt aattgactca gatactttaa attcatcgta ggcttgttca tctattgata
     7561 tagaagttcc ataaggtact ttaaattcct tagtctgacc atctctcagc ggaaaattct
     7621 cttgttgcaa cgtttcactt ggattaaaca agaagtcttt cgtcttatct tcatctagtc
     7681 caacgacagt tttacttact ctgacggtgt attctttagg ttgccaaaca gcatataagg
     7741 tatttgttgc atcagggttg ttatcaatac ctattgattg acctgctgta aattccacac
     7801 gtcctgtatc agctaaatcc ttatcatgat gccaaccaat aaggttgtaa cctgtccttg
     7861 taaagtattg gttttcagga attgtagttg tgctattcaa ctccatacgc ggtgtctcca
     7921 cttgtgttac cacattacca ttttctactc tagtaccacc gttaccattg tatttgatgg
     7981 aggtatcttc taatttgata tctcctactg gaataatgac aggttttatg gtgatatttt
     8041 tattagcatc tgctaaatgg gcgtcaatat caatggaatc atatgggtta taaattttac
     8101 cattgtacca ccagccacgg aaacgatagc catctggcat tgtcggacgt ctcagtaagg
     8161 ctgaatggga gccatcgtca taggaatcag ggtcagtagg ctcattactt gtttgtaatt
     8221 gctgaccaga agcatccaat gctggctttc catctgtacc aacagcatca ttgctgtata
     8281 taatatgata atctccagcc tttcgccaaa tagctcttaa attgatatct tgagttacag
     8341 cacctgaaaa gttataaggt ctaatactac catctggatt aacataatac caaccgacta
     8401 atttgtaagc gtcttttacg tacttgtact tagttgttgt atcaacattt gagagactag
     8461 tatctgtcgt ataataggca tctttagttg agtcgggatc tttatctcgt gaatcatact
     8521 tataataata tgtacctgaa ggatcttgga tataatccct tgtaatatct gtataatccg
     8581 gaatacgatc accataatgc aaatctaaat aggtatcatc tgtttttgat aatcggcctc
     8641 cgtttggatc aatattgaca cgatatgtta ccttttgcca acctgcatag actttaacat
     8701 catgaggagg catagtcgtg ttaaagtcaa atacttgtgt ttgtgcttgg tctttatacc
     8761 atttaccatc ccaaacatac cctggtcgac taggtttagg ttgaacagtt gtcgtatcgg
     8821 gggcataaga ggacaaattt tgctcatata gaacatcctt tactggaaaa ttaggaagct
     8881 ctgtattatc aagcggatct aaatatttaa tcttgtatga attacgttca taccatacca
     8941 ctaagttcaa ataatctttg tggtaggcac catatctatc atagtattca tctgaaatag
     9001 gaacttttac tcctgcactc gtttgacttg cgtactgatc aaaaaggtaa ttatcaggat
     9061 ataaacgttg ataatattta acattaaacc ctagatattt ttctgtaaag gtaaattcat
     9121 ctgccccagc acctccaccg gtatctgcta aagaataagt gccatccaaa ccttgtttga
     9181 agaaccgata agtttgaact atattccctt ttggaaagag tttgataaca tctgaatcaa
     9241 cggtatcatt aggtaaaatg aactcaccca aataactcat cccataggtt ccaacttggt
     9301 tatttccaac attggctaaa taaccccatg cacccgtctt ccattgataa ccattagcgg
     9361 ccaaggttgt accgtaaagt cctgtgtaag tatcagcatc agagctccaa ttatacgaat
     9421 agtaatattc tggcaaggaa tatccgccat attttgcaaa ttttattgtg atgagttttc
     9481 tatcataata aacattaaca acacttgaac catcgtcttt tatcatgaca gaagtttctg
     9541 tcctcgtatt attaacttta aagccagtcg gtagtttatc attaatatct tgttgtgtta
     9601 gcgtctgatt agataaagat aggcctgatc gtgttacttg ccctgcgtac tcatatgtct
     9661 tttgcgcatc agtagcattt ttattatccg ttgctgattg ttgccagtag tttatcgtgt
     9721 aggttgtttg tgcgggcgac caatgtgcat atagcgtcgt atccttggtc aagacttgat
     9781 taaaatcaaa ggctgcccca ccactagcag ctgtgtacca ccctgcaaaa gtataacctg
     9841 gcctcgttgg atcattaggc ttaattgtcg aagcaggttg gcctgttaac acacgacgag
     9901 gtgcaatata ggtaactcct gttgataagt cgcctgtgtt gaattcaaca ccgtacgatt
     9961 ctttaaagac aggataaaga cttatgtctg aagatacagg taatgcttga atttctgatt
    10021 cagaaagtgg agctccattt tgagttttag accaacctac aaataacaat gttgaagtgg
    10081 gtggtgaaac tttaaatgaa cttaaatccg cggttccgtt tcctgaacta tccaatggta
    10141 cttgataacg ctccaaaata cttttaccag cagaatcatc atataaagtt actgtcgcca
    10201 ctttactcat aaatggacga acacaaattt cttttgtctc agttacagtt attggctcac
    10261 caaatttaac agggtcacca tactttccag tagtaggatc ataggtatac caaccattaa
    10321 aatgctctcc tgctttaatc gtcggaatcc caacttctcc tagagattct ccatctttta
    10381 taatttgatg atgaacttgc atacctgaag ctgtcaggaa atcataatca gttccgtcat
    10441 tattttgaaa atggtaagtt aacctaggaa cttctgtatt atcctcttga acaattgcat
    10501 aaatggagaa tgaatctgtt ttaaaagcaa catcactgct agtgttctta gtttctgcag
    10561 tatctttaga ttttaatact tctgtttgac catcatcttt aaagtgaaca actttaaggt
    10621 tttcatctga agcttctaat ggcttatcat agttaacctc tacttttact ggagcttggg
    10681 gttctgcttc tttaccattt gactcaatag taatgtcata gagtttgaaa tttttgattt
    10741 cactatcttg tttagcaacc tctgtcaatg cttttttctt ataatcttta aaagtagctg
    10801 aattgtcttt taattccgtc acctttaaat cagcattttt aggaatctta gcttctttgg
    10861 tcaaagtcac tgttacagta tagtctgcac ctctaaacat caatggttct tcacgataag
    10921 cagcttcctc agaagatgat gtttctgtta cactagaagc aggagtctgg ggcttgctct
    10981 gctcaacact tgattgagaa ctagatgttg atgaagttac ctggctagaa tttttatttt
    11041 ctaaagtaat ccccacatca tctgtcttag tttcttcaac tgttattgct ggtagaatta
    11101 aaaaataagt cgttaaaaaa gttgttagga tcatcaatga ccacatgata attttccact
    11161 ctttagggtg ttttttcttt tttaatgatt cgattataaa agtttgacac ttctttagca
    11221 tttttgcatc ctccctaacc ttaattgata ctactaatct tacctagagg ccatattctg
    11281 aaagaaattt tacctacaat ttgttcttct gaaacatctc ctacagaagt acttcgagaa
    11341 tcaattgaag tttttcggtt gtctcctaat acaaaaattt ttttatcagg tacttgatat
    11401 gggtatttta tattactatt accgagtgct ttatgaataa catatggttc tttcaactta
    11461 tgttgattca cgtaaacatc cccttgagaa tcaatattaa cccagtctcc tgactctgca
    11521 ataacccgct tgactaggac cttattattg tagtaaaacg cgacaacgtc tccagtttta
    11581 aaatttgaac cttttactgt aaagactaca tcacctgcac ttaaagtctt attcattgaa
    11641 tgtccgtaga ttcttaatac aggcaaccat aaaaccgcaa ttaaaatggc tattgaggca
    11701 accgccatca agatgtatat ggtattttta atgacactcc aaaagcgttt ctgataagtt
    11761 acgcgatcca gttcttgaga taatttatct gaactaatct gtcttttcat tgtctacgcc
    11821 tctcttatct actaaattct ttacgttttc taaataggta tctgcagcta actgagcagt
    11881 ctcaaaaata ccactaagca ttaaggaagc ttcggcaata gaaccagctt tgctaatttt
    11941 gattttttta tcatctagcg cttctttaag ttgctgaatc tctttctctt gtttttcaat
    12001 aagaagttgc tgttctaaca taatttctag caagtctttt ctttttaatt tttttaaatc
    12061 ttccatcgcg attacttcct taactgaacc ttaaattatc gtttagatat tatatcaaag
    12121 ttctaacctt taaactcatt ttttgtcctg tgttttttct caaaaaagtc tatgctaaat
    12181 taacattttt gataattttt tgaaaaatct catcgaagtc attttctttt tgaaagctcg
    12241 aattctaggc attaaaaagc catatatcaa attgatatat ggcttttttt attatttaaa
    12301 acaaaagaat caataggaca atagcgtcaa tttagtgaca taatctatta cagattaagt
    12361 tctttttgaa taatataatc caacttttca actgtttttt cccatgtgaa atgttcttta
    12421 attcttttag caatattctg ttgtagtttc tctcttaatg ccttatcttt tactaataaa
    12481 tcaagagatt catggagtga ctgagtattt tcttccatga tgattcctaa ctcagggcta
    12541 tcaataactt caactgttcc accgcgatct gttgcaataa tagcactcga aagtagacca
    12601 gcttctaaaa tagaggttgg taatccctct ggatacattg atgggtaaac aaagatatct
    12661 gtctgtgcca ttaaagacat agtctgttca aagtttaatt tccccaaaaa gttaatctgt
    12721 ttggactgat atttctcttt caaatgtgct aattcaggtc cgtctcctgc aatctgtaaa
    12781 taaacatttt cagagtactg tgacatcgaa aatgcttcta agagcaattc aatgcctttt
    12841 tctttaataa ttctaccagc ataagtgatg aaaatatcat cagcagattt ttcaaggtaa
    12901 gccgtgtcag caaaatcaga acctagactt tcagataccg aattataaat aactccttta
    12961 gcttctatat taaaatgttt taaccactca acgcttctct tagataccgc ataaaaatct
    13021 ggacgatagt gcttaacacg cgctgtgaga agatgttcat agatagctcc aaagaaatct
    13081 aaaaaacgat tattgacaga aaaatgactt gacccatggt ctaaaacaat actaggtaaa
    13141 cggtgcttct ttgcaaaaga tagcccttct agcgttgtta actgaaaacg tgtattacaa
    13201 atcacaaaat caatattttc atctgaaaca tgtttcatca gcgtgttgta ttctcgattt
    13261 ttgttaataa taggatagcg ctgcttgaca atatttttgg tcggtaaacg gtaaattttt
    13321 ctacccttgt cttcatctat aatcggtaaa tcatcatgat tagttgttac aataacaaca
    13381 cggtagccac gcttaaccaa atctgctgtc attttatctg tataacgttc aatacctccg
    13441 aggaagggta gataatatcc tgagaaaaca gcaactgttt ttaccttatt ttccatattt
    13501 atccactttc atcaataagc catcttttaa gcctttaatc atagcaacta attttttgct
    13561 cttttgctct tctgctacca acactcgaac aaattcattt cgcataaata ctaaatattt
    13621 gtgccgcttc ttcttaccat atttttttat aatataaatc gcattgcgta tcatgtaata
    13681 ttttcgaaat ggtgaatgat tcaatacatg aaaaacatgg ccaaattttt taactcgtga
    13741 agagtgtcca atttcgtgta aaagaccaat aaaattaacc tgataagtct tatatcccat
    13801 ctctgacaga cgataattca tttcagagtc aacaaaatca ataaacatct cttctgcaaa
    13861 gccagatgtt tcttcaaaaa cgctcgtttt cattaaagca gccgaagtaa tacactcttc
    13921 aatttcttta tagtcaaatt cttgcataac taaatcttca cggttcatat cttgatacaa
    13981 acaagataac ataccgacct taggtaaaag aaggtaattt tcataattat ctatcaaatc
    14041 acctaggaca accgaatctt gatctaaagt caagaaccaa tcaaattctt gtgctactgc
    14101 aaattgaccg atacagttca aagcatatgc aattccttta ttttctgtta aataatcaac
    14161 agttaggtgc ccctcttcat tataatcggc cactaattga gaaatttctt ccttattttt
    14221 cgagccatta tctacgatgt agatatggct tacttgagga taaattgctc gaatgttctg
    14281 atctaagcgt tcaatattgg ggttaaaggt gacaataccc gctaaatatt tcatgttcta
    14341 tgctcttttc taaaatctct aaataactga atgactggtg ctttggttat aaaaacgata
    14401 ccgacataga tagttactgc tactaaactt tgaatgacat aatttaccaa tgatactgac
    14461 atttgagtat tgatataata aagtacagct ccactaagag tagcagcaat taaatagcgc
    14521 agcattcctc ttgttaattc tttaaaagta aatacatctc ttaaagagat agcttgatag
    14581 agggagacaa taaattcagt aataactgta gagataatag ctcccatagc acctaaaatt
    14641 ggtattaaaa gtatattaag cacaacattt gccacaagtc caataactgc agacattgtg
    14701 taagcttttg tacgtcttga agccagtaga tactgtgtcc ctaaagcgtt accataagaa
    14761 atgcaaatga tcatcaaaga ctcaacagcc attagcggac cgacagcttc ataccctttc
    14821 ccaaaaaaga agacggcaaa tgtcgacgaa actcctacca ctcccgcaat cattaacatt
    14881 gagatagcat tgcttaaatc aacaagtttc aaaagtaaag ccttagcttg cttttctttt
    14941 cctgaagaaa atagactgga gagccttggt aagaaaactc ctccgattgc tgaaacaatg
    15001 gtaaataaaa tacgaataat tttatcagat tgatcaaaat atccagcact gaccacacta
    15061 tccattgctc ccagcatact cttgttaaga gagagataaa tctgcatggt gatttggggt
    15121 aaaaataaca ttaaagttgg tcttagatgt attaagatat ctaatctact aaatggtatc
    15181 ttgatgattt catgctttaa atgatgccag actgttaaat ttccgagtaa tgatgcaaat
    15241 gcaattaaaa acatataaag tgctaggtca tctggcgtct tcacaactaa gaaggtcaaa
    15301 acaactgtaa taagcttaac aatggtatta cgaatgacaa ttatcttaaa gtcttcaact
    15361 ccaataaaat accacgaaat atcagttgca gtcgctaata aattaatacc ttgaattaaa
    15421 tagaataatt gccattcgtt tgtaagtaag acaaatccaa agaataataa cgttgctata
    15481 gatacagagg caagttttaa tacaactaat tcccaaaata tcttacttct cttatactta
    15541 tttgactgca caaaagctat ctcacgatta ccgtacaaag atatccctaa agcaccaaat
    15601 aaagtaaagt acatgacaat ggagctcgta taggcattaa taccgatgcc ttgaggagat
    15661 agtacacggg aaacataagg aactgttact aaaggtaaca acaaagtcaa taactggtat
    15721 gaggtattat aaaacatatt tttcaaaagt ttcattagtt tctcctcata attaaactaa
    15781 taacagtatc aaataacaat aaggcatttg atacaattac caagccagtt aacgcatacg
    15841 gcaccacttt ccttcctgcc tcatttagac gcgctggaat acttgatgca aaaacttgta
    15901 ccaaaagcaa tgtaacagta aagtaacgtc cttgaactcc ttcaatgtag cttgttggcg
    15961 ctccacgata ttctaacatg ataaaaaaga caccaagaat tcccacaaag aaactagcta
    16021 agaaaagaaa tcccatattt tttgacttaa caaatttttc ttcagaaaac catactagaa
    16081 agaccatgag ataccatact gatgataccc aagtgggaat aaaattctga cttggatatg
    16141 gagaggcaag ccaggaagta atgtcattac ttaaaatatt gataccagca taattatagc
    16201 caaatgtcat tacataacgt cttaaaacag caaggacacc accatgtctt gcggtcagaa
    16261 cataaactat agcaagtgac aaaaaggtta gacatagcca aaatagcttt ttagatttaa
    16321 gaccttgaaa taaatgtgat agcttctgat tatagcccat taatttagga ttgttaacct
    16381 gcaataatac cggtattact agcaataaaa acagccagta attctgctta catgcaacaa
    16441 tgactaaaag aatcaatcct ataagagcaa tactacgatt tttagtcaac gatttatcaa
    16501 ctaaaagatt tatcaaaagc gataaaaatg ctccaaatac aatatatcca gtcgagtcat
    16561 aagatagact agcaaatgaa tttagaacta cgggactgag gaaaatagca aagtagatta
    16621 actttccttt tttcacatac cggataatga gggcaaccaa ggtgattgag acaaaggaac
    16681 taaataacct tgctactgta atcattaccc ccatagaagg atacacaaat ttgcctaaaa
    16741 ataagccaac tgctggcaca agatgacccc aaaaaacaaa gttatgttta gaatagttta
    16801 tctcacgagg caaatgatta ccatcaacaa aattggcttt atttaaatag tatttttcaa
    16861 atctagtacc atctctatag gaggtcgatt gtcgtctcat acctgaatca atattatatt
    16921 ctccatagta agaagtatca ataatgttat ttgaaatacg accagacaca gctaaatggt
    16981 attgtccatc aggttcattg aaaaatggca ttaataggct caatacaata gtaatcgtta
    17041 agcctaatat aaaatatgtt ttatgcaagt tcttgtctag aaaaaacttt tcaaatagac
    17101 gaactttttt gaggttcata ttaactcctt tttatatctt ttaataaaaa caccaagtca
    17161 cgaatgcgat ttgaaaagct atcatagaac cgttttttag tcatcatttg tttaagtatt
    17221 gctacaaaat ctttattttg agcattttta taacgttgct gattaaaatc cagataagct
    17281 ttagcttccc ttaattgact agagctactt ttttctaaaa atggtacaag gatgatatag
    17341 taactcaaaa caaaatacag ttcttgtaaa tgcctttcgt aacttgagct catattcaag
    17401 atattcccac ttgcattctc agcatgtcgc ttatgttcac caacacttct gtttaaattg
    17461 taacctgaac caatgacacc agatacccct gcaaaaagat tatcatgtga caaaattaaa
    17521 ttatcactgt aaaaagtcat tacttgatca acaaacgact tgcgtaaaca aaaagtccaa
    17581 ccttgacgat agttatgata tgaaaagtct aatggatact tagaaattgt ttcctcacca
    17641 ttgaacttaa aattattggg aatagtagcc tcttctgata actttttgat atcaatatcg
    17701 gcgctaagaa ggtttatatc ttggcgttct tccataatat caacttgcat cttattttta
    17761 tgatgatacc atgtgtcatc ttgatcacta aaaaagacat agtcaacctc ataggctaac
    17821 acatcaataa gtaattgacg aaaatttaaa cgccaaccta aatttttatc attactagtt
    17881 attgaccaac catctaaacg gtgctctttt atatagtctt caaccacctt tacagtatca
    17941 tcagtagagc catcgtccct cattaagaca tagtctggtc tcaacgtttg atttctaatt
    18001 gaatctagct gatcgtggat gaaaccttga ccattatatg ttgccatcaa aactgctgtt
    18061 tttaccatat tattccttac tttttaaacg caaataataa atctctcata cgtccactat
    18121 tagagttata gagtcgccaa tttttaatca caaaagcact attatgaatt aatgacaatt
    18181 tagaagcttc caatctttct ttttcaaagg ctaagcgttg atttaaagcg acatcatttt
    18241 gatatttgtt gacaaccttc tgcatggttt cgtaaaaatc aacaaatgtt tttaatcctg
    18301 tgatatgttg tgttttaggc gacttccaat tcaaaacaaa tttgctatta ccacttgcgt
    18361 ttcctccata tcgaatatga actcctacgc tttcgttaag gttataacca tttccaataa
    18421 tactggcagt tgtctcgata agattatcgt gcgaaatatt agctccaaaa gtccaattat
    18481 cagcgatagc ttgcgcaaaa tctctcttca tagcaatggt ccatccggga cggaatgttt
    18541 gataatcata tactttagga tactgactga ttctatcact agaagaaaaa gttagaaaat
    18601 gtggaacact ggcttctgta gacatcgttt tgatatcaac gtctgcactc aaaacctcaa
    18661 tttgagggtt atctgacata atggcaaact gtcgttcgtt tttatcaaga taccaaatat
    18721 catcttgatc actaaaaaag acatagtcaa cctcataggc taacacatca ataagtaatt
    18781 gacgaaaatt taaacgccag cctaagtttt tgtcgttttt aacaattttc cagccttcta
    18841 actcatgttt tgcgatatag ttattgacga cattgactgt ttcatccgtt gaacaatcat
    18901 ccctcaataa tacataatct ggttttaatg tctgttggcg aattgaatca agttgttcag
    18961 atataaattt ttcgccattg tatgtcgcca ttaaaactgc tgtctttacc atattactcc
    19021 tcaaatgctt gtactgtttt taatattcca gattcaagag aaacttctcg cttccatccc
    19081 aacgcttcta atttggccat agtgagacgt gttctcccca ttttgctata acctgcactc
    19141 atagttttag gaatatcgaa tactactgaa atatttttat cagaaaagag atcaactaac
    19201 ttttgtgcca aatccttaat cataatgggt tgatcttcat ttgctacatt gtaagcttga
    19261 ccaacttcac catttaataa aatagtaaat aaccctgata cagcatctgc taaataacag
    19321 aaagcacgct ctgctgtacc actacttttt aaaacgatat ctttgccatc aatgacattc
    19381 gataaaagat cattcataat acgaccatcg ttaccaagct ccataccagg accaaatgaa
    19441 tgtgcaatac gagcaatagt aaagggtacc ttatattgat catagtaact ttgcagtaga
    19501 gtttcagcca tacgtttgct ttctgggtaa caagctctag tagctaaaat atcaaatcca
    19561 ccataagctt cttcatcaat cacttcttta atagatgttc catatacttc acgcgttgat
    19621 aagaacaaga agttttcaat tgttttttcc tttgcaaagt ctaataaatt caatgtacca
    19681 attgtattag cttttatgat gctaacagga ttactcaaaa tagcagttgg actagcattg
    19741 cttgctgcat ggataatgta atccactttt ccatcatact ctaggcgttc ttctacatca
    19801 taaggaatta aggtgaaata atctttccca actaaatctg aaaatttatc acgggctttt
    19861 tcaatgtttc tagctgtcgc aataatttgc gtttggtaat ttcttgtctc attgagatag
    19921 gctagtaaaa ataccatata actcgctaac atactattag aaccacttat taaaacgcct
    19981 ttattagcaa ttctttccca attaatataa ttttggttac taataagtgc taaatcttct
    20041 tgtacaattt tattactaaa tttaatcatc attacatccc ataaatttgt tcattttctc
    20101 tagcatcata caaagcttta aacatataaa aatcatctgg tgttgttatc ttgatatttt
    20161 catatggtcc ctcaacaata gtgagttcac gattgtacat acccatcaaa gtactagaat
    20221 caattgcatc agtaatccct ttgctaatgg catcacgttc aacagaaagg atatctgata
    20281 gataaaaact ttgtggcgct ttagcaatga aggaacgagt acgatcaaca acttcagata
    20341 ttttagaaga atcattgact agaacaactg tctctttagc acgaactgaa gttaccgcac
    20401 ttcctgtttc ttttactgac tgtatattag cattaataac ttcttcatta atcaatggac
    20461 gtactccgtc atgaattaaa acaacggcat catctgtagc cagctgttca gcggcttcaa
    20521 gccccttaaa aattgacatt tggccagttt ctccacctgc aacaactgct tttactttcg
    20581 taaggttgaa acgctctacc agattattca tataatctag ccagtctgat acacagacaa
    20641 ctactacagc atcaatagct tctgtatttt ggaaaatatc aattgtgtgt acaataatgg
    20701 gtttaccatg cacttctagg aattgctttg gtttaccttt ggtattcatg cgacgaccaa
    20761 caccacctgc aaaaataact ccaatattca tcattaacct tctttccagt accaagtgta
    20821 ataactatga ctttctctct tactttctgg aggtaattcc atataactac ccccatataa
    20881 ttgattcaga taagcatgct catttttaat ttttcgggtt ttaatgtttt caaacataac
    20941 atcttcatac tcagaaaaaa tttctcttgg aaaatattct ttcaagcgat atcttgaatc
    21001 ggtataaccc atgtaaggaa cctcttgatt attatatgtt tccatcactt gatcaacaat
    21061 ttcagcacgt tttttagctt ggccgtgata agcgatgaat cggggtagcc ccaaaattag
    21121 ccttgcattt gcataaagct ttcgggaacc gctagcaaaa ttggggtaag tcgtacacat
    21181 caaatttgca gcagcagagt gaactttttt aaagaatctt tgacgttctt ctgcactctc
    21241 tggtaatcgg tcaagtggga agatatcaat aaaaatccct gtccaaggat gaattctatc
    21301 tgttttactt aagtacattg actttgtgtg atacagcttg gcaaatggcc atagattttt
    21361 ttcaacagaa tgatgtagca atttaaaatc gctattatta gcatcagcta atgctttcat
    21421 caaacgctca tactggcttc tttctagcat caaatcaata tcgtcatccc atggaatgaa
    21481 tcctttatgt ctcattgctc ccagtaggct gcctccgcct aaagaatatt caattttatt
    21541 atcacgcgca actttatcaa tataagctaa catttcaagt tgaacttctc ttatttctga
    21601 gaccgtcatt tctttcataa ttacctctgt ctaacagtta actcacattc ttttaaatac
    21661 aaatcttata gctgtaatat agaaaataaa taaagctact gcaaaaagaa tcactagggt
    21721 catgggtttg aaagttttct ttgttttttg accttttgga gtgtttgcaa gtgcattgta
    21781 ttcttgtttg ttgtactgca tgtccattat ataaccattt ata
//
LOCUS       DATRQA010000018        20714 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.018, whole genome shotgun sequence.
ACCESSION   DATRQA010000018 DATRQA010000000
VERSION     DATRQA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.018"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(34..744)
                     /locus_tag="VBD27_000502"
     CDS             complement(34..744)
                     /locus_tag="VBD27_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7024537.1"
                     /translation="MNIKRHSIFLWLTTSILAILMAVGMTIYFNTYQASPLARRIAKT
                     ATNHKNYLLFRSKGKVKANIIFYQGALVEEEAYSQLARDLADKGYNTYILKTPLNLPV
                     LSPYKAKTIINQNHLTNVYLAGHSLGGVVASQNAKVAPVRGLILLASYPSSKSDLSHK
                     KLRVLSITASNDHILNWEKYEEAKKRLPNSSTFRTIVGGNHSRFGNYGHQKGDGKATL
                     SHKSSEKQLATFISNFIH"
     gene            complement(741..1439)
                     /locus_tag="VBD27_000503"
     CDS             complement(741..1439)
                     /locus_tag="VBD27_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001907086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7024538.1"
                     /translation="MPFIFRHSFFNKVLIFWYTIIMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDTFDNPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPSHYSVHPGHGMN
                     TTIGHEKNFNPFFQ"
     gene            1607..2371
                     /locus_tag="VBD27_000504"
     CDS             1607..2371
                     /locus_tag="VBD27_000504"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEO7024539.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGVLDYNEETAQAAVD
                     KLSPEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGIAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIVHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            2479..4986
                     /locus_tag="VBD27_000505"
     CDS             2479..4986
                     /locus_tag="VBD27_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEO7024540.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLVKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEQLFLEGFELRTPRIDTVELSQVFYPCLEKYSLGALAESLNIEL
                     TDAHTAIADARATAQLFIKLKAKISSLPKEVLETILTFADNLLFESYLLIEEAYQEAD
                     FVNPKEYYFWQGLVLKKEKAVGKPKKLSSDFQVNMALLGMDARPKQIVFADLVKAHFN
                     DQTTTFLEAQSGLGKTYGYLLPLLDQSQKQQIIVSVPTKILQDQIMAKEIKHIQELFH
                     IPCHSIKGPRNYLKLDAFYKSLQVQDRNRLINRFKMQLLVWLTETTTGDLDEIKQKQR
                     LESYFDQLKHDGEVTQSSLFYDLDFWKRSYDKVAQSQLVIINHAYFLERVQDDKDFAK
                     GKVLVFDEAQKLVLGLENFSRGQLDISHQLQVIQKIIDSPIPLLQKRLLESISYELSH
                     AAELFYRHNSFEFSETWLKRLKNSINALEVVGLDDLQTFFTATYTNYWFETDKVNEKR
                     LTILRGAREDFLKFSEFLPPTKKTYMISATLQISPKVYLSDLLGGFSSISTEKIAHEK
                     NANQKVWIDTSMPNILDLSPEQYAYEIAKRLQDIMTLKQPTLVLLTSKQTMFMVSDYL
                     DKWEIKHLTQDKNGLAYNVKKRFDRGESNLLLGTGSFWEGVDFVHRDRLIEVITRLPF
                     DNPKDYFIQKLSQSLTKEGKNFFYDYSLPMTVLKLKQALGRTTRREEQKSAVIILDSR
                     LVIKSYGQTIMHSLGRDFEISKEKINKVLTEMAKFLI"
     gene            5072..6265
                     /locus_tag="VBD27_000506"
     CDS             5072..6265
                     /locus_tag="VBD27_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7024541.1"
                     /translation="MTYLSERVLNMEESVTLAAGAKARELRVQGRDILSLTLGEPDFA
                     TPKNIQQAAIEAITDGRASFYTPSSGLPELKSAINAYFERFYGYSLKPNQVVVGTGAK
                     FILYTFFMTVLNPGDEVIIPTPYWVSYADQIKMAEGKPVFVTAKEVNHFKVTVEQLEA
                     VRTDKTKVILLNSPSNPTGMIYKAEELEAIGNWAVEHDILILADDIYGRLVYNGNIFT
                     PISSLSESIRNQTIVINGVSKTYAMTGWRVGFAVGNHDIIAAMSKVVSQTTSNLTAVS
                     QYATIEALNGSQESFEKMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYLFPNVTKAM
                     EMKGYTDVTAFTDAILEEVGLALVTGAGFGAPENVRLSYATDLGTLKEAVRRLHVFMG
                     SNEIN"
     gene            6286..7632
                     /gene="asnS"
                     /locus_tag="VBD27_000507"
     CDS             6286..7632
                     /gene="asnS"
                     /locus_tag="VBD27_000507"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO7024542.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEVIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYSTYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYIAEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIKP"
     gene            complement(7672..8229)
                     /locus_tag="VBD27_000508"
     CDS             complement(7672..8229)
                     /locus_tag="VBD27_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7024543.1"
                     /translation="MTKSPARLISFISIAIAINLIGANLALFLRLPIYLDTIGTLLIA
                     VILGPWYAASTAFLSALINWMTTDIFSLYYSPVAIVVAIITGILIKRNCKPSSLLWKS
                     LIISLPGTIIASVITVILFKGITSSGSSIIAQFLHGIGLDMTSSLILVQVGTDYIDRL
                     ISLILVFSTITLLKKHSPNLINQRF"
     gene            complement(8226..9209)
                     /locus_tag="VBD27_000509"
     CDS             complement(8226..9209)
                     /locus_tag="VBD27_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO7024544.1"
                     /translation="MNKEKIIIDCDPGIDDTLALMYAIQHPKLEVVAITITAGNSPVE
                     LGLKNTFVTLELLNRRDISVYVGNNLPLQREFVSAQDTHGMDGLGENNFTLAQPIIFQ
                     EESADCFLANYFEHKNDTSIIALGPLTNIARALQTNPKLGKHCKRFVSMGGNFKSHGN
                     CSPVAEYNYWYDPHAAQYVFENLDKKIEMVGLDITRHIVLTPNHLSYMERINPDVSTF
                     IQKITKFYFDFHWQYEHIIGCVINDPLAIAYFVNENIATGFDSYTDVACHGIAMGQTI
                     VDQYHFYKKDANSKILTSVNTNLFWNDFMTVLLHAQNSVIIEDLEMLGLNK"
     regulatory      complement(9353..9397)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            complement(9455..9868)
                     /locus_tag="VBD27_000510"
     CDS             complement(9455..9868)
                     /locus_tag="VBD27_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO7024545.1"
                     /translation="MLYEVTSSNTQGVDGKVYLSNGKIVETNHPLNHLPGFNPEELIA
                     LAWSTCLNATIKAILEQKGFKDLKSRVDVNCQLMKEKQVGKGFYFQVNAVASIEKLSL
                     SDSKLIVNKAHSRCPISKLISNAKTINLDTVKWSV"
     gene            10022..10912
                     /gene="rapZ"
                     /locus_tag="VBD27_000511"
     CDS             10022..10912
                     /gene="rapZ"
                     /locus_tag="VBD27_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEO7024546.1"
                     /translation="MSDEQIKLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLVPK
                     FLELAAQSGDTSKIAMVVDMRSRLFFREINSILDSLEINDNINFKILFLDATDTELVS
                     RYKETRRSHPLAADGRVLDGISLERELLAPLKSMSQNVVDTSELTPRQLRKVISEEFS
                     NQDSQSSFRIEVMSFGFKYGIPLDADLVFDVRFLPNPYYKPELRDKTGLDTEVYDYVM
                     SFDESDDFYDHLLALIKPILPGYQKEGKSVLTVAIGCTGGQHRSTAFAHRLSEDLKAD
                     WTVNESHRDKNKRKETVNRS"
     gene            10909..11883
                     /locus_tag="VBD27_000512"
     CDS             10909..11883
                     /locus_tag="VBD27_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEO7024547.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRLEDVEITAVVTVADDGGSSGE
                     LRSVMQLTPPGDLRNVLVALSDMPKFYEQIFQYRFAEGDGDFAGHPLGNLIIAGVAEM
                     QGSTYNAMQSLTQFFHTTGKIYPSSEHPLTLHAVFKDGHEVVGESQIADYKGMIDHVY
                     VTNTYNEETPTASRKVVDAILESDMIVLGPGSLFTSILPNLVIPEIKQALLETRAEVA
                     YVCNIMTQRGETEHFTDADHVEVLKRHLGQDAIDTVLVNIEKVPESYMENNHFDEYLV
                     QVEHDFSGLRKHARRVISSNFLKLEKGGAFHHGDFVVEELMNLVGTHL"
     gene            11880..12791
                     /gene="whiA"
                     /locus_tag="VBD27_000513"
     CDS             11880..12791
                     /gene="whiA"
                     /locus_tag="VBD27_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEO7024548.1"
                     /translation="MSFTVKVKEELLGHKSENKMELSAIIKMSGSLGLANHGLNLSIT
                     TENAKIARHIYSMLEEHYHLQPEIKYHQKTNLRKNRVYTVFIEEKVDVILADLKLADA
                     FFGIETGIEHSILDNDENGRAYLRGAFLSTGTVREPDSGKYQLEIFSVYLDHAQDLAN
                     LMKKFMLDAKVIEHKHGAVTYLQKAEDIMDFLIVIDAMEARDAFEEIKMIRETRNDIN
                     RANNVETANIARTITASMKTINNIIKIMDTIGFDALPSDLRQVAQVRVAHPDYSIQQI
                     ADSLETPLSKSGVNHRLRKINKIADEL"
     gene            12806..14203
                     /locus_tag="VBD27_000514"
     CDS             12806..14203
                     /locus_tag="VBD27_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEO7024549.1"
                     /translation="MACTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLKVMTSKDQ
                     PRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDGIWGEAGINSKNVAMSATETITTNSR
                     VLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIAFSDTEEI
                     WWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFIEQYHLDL
                     TYSNEHFNPRYAFGSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWCQKPYRKI
                     TVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRAFRSVGINRTSQTSILQLRPNKSLETT
                     GVQWLSYGSMPFATMVPLFTQVETVPDYFSNTTKDASTDNFYWTNRLIAALADPHFYQ
                     HEADIESYIERTMAQGHAHINGIDREVAENKEIDFQQKNQEMSDYIQKESQELLNRIL
                     FDASNLMTNRFSMGD"
     gene            14345..15865
                     /locus_tag="VBD27_000515"
     CDS             14345..15865
                     /locus_tag="VBD27_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEO7024550.1"
                     /translation="MRKKFLLLISFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEF
                     TKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAAIQDSNAFVYMDDNMETWAPKVAKSV
                     KSKKVTTIKGTGDMLLTKGVEEEGEEHEGHGHEGHHHELDPHVWLSPERAISVVENIR
                     NKFVKAYPKDAASFNKNADAYIAKLKELDKEYKNGLSNAKQKSFVTQHAAFGYMALDY
                     GLNQVPIAGLTPDAEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGVKT
                     AVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLKKTTDVAGKEVAPEEDKTKTVETGYFK
                     TKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYYTAGYKTD
                     VESIKIDGKKHQMTFVRNGKSQTFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQFK
                     YIQFSDHGIKPNKAEHFHIFWGSESQEKLFEEMENWPTYFPAKMSGREVAQDLMSH"
     gene            complement(15978..16238)
                     /locus_tag="VBD27_000516"
     CDS             complement(15978..16238)
                     /locus_tag="VBD27_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEO7024551.1"
                     /translation="MKKDIHPDYRPVVFLDTTTGYKFLSGSTKSTKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAQ"
     gene            complement(16347..17282)
                     /locus_tag="VBD27_000517"
     CDS             complement(16347..17282)
                     /locus_tag="VBD27_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEO7024552.1"
                     /translation="MIIFQQILDKIKEYDTIIIHRHMRPDPDALGSQIGLRDIIRHNF
                     PKKKVLATGFDEPTLAWIAKMDQVTDQDYQGALVIVTDTANTPRIDDERYKKGDFLIK
                     IDHHPNDEVYGDLSYVDTNASSASEIVTDFALSCDLLLSTSAARVLYNGIVGDTGRFL
                     YPATTSKTLKIASKLREFDFDFSAMARQMDSFPFKIAKLQGFIFEQLEIDKNGAACVT
                     LTQEDLKRFDVTDAETAAIVGIPGKIDIVESWAIFVEQSDGHYRVRLRSKSHIINEIA
                     KRHNGGGHPLASGANSYSLEENQAIYQEIQEVLSL"
     gene            17549..18571
                     /locus_tag="VBD27_000518"
     CDS             17549..18571
                     /locus_tag="VBD27_000518"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO7024553.1"
                     /translation="MTQAVLKELAKAELHCHLDGSLSLPAIRKLANMADIILPNSDKE
                     LRKYVIAPAQTESLVDYLKTFEFIRPLLQTKEALRFAAYDVARQAALENVIYIEIRFA
                     PELSMDKGLTASDTVFAVLEGLADAQKEFNIVARALVCGMRQSSHKTTKEIIKHIVDL
                     APKGLVGFDFAGDEFSYPTDSLVDLIQEVKRSGYPMTLHAGECGCAKHIADSLNLGIK
                     RMGHVTALTGQRALIKRFVEEDAVAEMCLTSNLQTKAASSIQSFPYQELYDAGGKITI
                     NTDNRTVSDTNLTKEYSLFVTYFGTKIEDFLVFNQNAVKASFTSDSEKDTLLHKLQEN
                     YDSYLK"
     gene            18630..19073
                     /locus_tag="VBD27_000519"
     CDS             18630..19073
                     /locus_tag="VBD27_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEO7024554.1"
                     /translation="MALAKIVYASMTGNTEEIADIVADKLRDLGLDVEVEECTMVDAA
                     DFEDADIAIVATYTYGDGDLPDEIVDFYEDLAEVDLSGKVYGVVGSGDTFYDYFCKSV
                     DEFEAQFALTGAQKGADCVKVDLAAEDEDIENLEAFAEEIASKLN"
     gene            19150..19425
                     /locus_tag="VBD27_000520"
     CDS             19150..19425
                     /locus_tag="VBD27_000520"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO7024555.1"
                     /translation="MELEEIRQEIDEIDQQLVSLLETRMGLILEVIAFKKKHRLPVLD
                     NNRENEVLNNVLKKVQNHQFDDVIRATFKDIMTESRVYQKENIVDGD"
     gene            19418..20614
                     /locus_tag="VBD27_000521"
     CDS             19418..20614
                     /locus_tag="VBD27_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEO7024556.1"
                     /translation="MIKRFDNYAQYVLVLGFSLLIGGVVGAITAVFGRVLLFLTAFRS
                     DYIAYLLPFLSIVGLFIVFVYQKFGGKSVKGMGLVFEVGHGNEETIPKRLVPLVILTT
                     WLTHLFGGSAGREGVAVQIGATVSHYFQKYCRLQNASQLFLVMGMAAGFAGLFQTPLA
                     ATFFAIEVLVVGRLMVSYILPSLIAALTANFVSHSLGLEKFSHSIATSMALTPDIILK
                     LLVLGLCFGLCGNLFAYLLAKAKLIASSRLLNPYKRIFTLGLLATFLLFIFHFGRYSG
                     LGTNLIEASFTNKNLYDYDWLLKLCLTVITLAAGYQGGEVTPLFAIGASLGVIIAPIL
                     GLPVILVAALGYTSVFGSATNTLLGPILIGGEVFGFANTPYFVIVCLVAYSISHAHTI
                     YGAQSR"
BASE COUNT         6659 a         3269 c         4159 g         6627 t
ORIGIN      
        1 tttcatctct ttttattaat tgtagatatc atgctagtgg ataaaatttg aaataaacgt
       61 agccaattgt ttttcagatg atttgtgaga aagggtagct ttaccatccc ctttttgatg
      121 tccgtagtta ccaaatcggc tatggtttcc cccgacaata gttcgaaaag tacttgaatt
      181 tgggaggcgt tttttagctt cctcatattt ttcccagttt aaaatatgat cgttacttgc
      241 tgtaatggag agtactctta actttttatg agataggtcg gatttacttg aagggtagga
      301 agctaataat atgagccctc taacaggggc tactttggca ttttggctag caacaactcc
      361 tcctaaagaa tgtccagcaa ggtaaacatt agttaagtgg ttctgattaa taattgtttt
      421 agctttataa ggtgataaaa ctggcaaatt taaaggtgtc ttcaaaatat aggtattata
      481 ccctttatca gctaaatctc tagcaagttg agaataagct tcttcttcca caagagcccc
      541 ttgatagaaa ataatatttg cttttacctt acccttgctt ctaaaaagaa ggtagttctt
      601 atggtttgta gcggttttag caattctcct agctaatggg gaggcttggt aagtattgaa
      661 gtaaatggtc atcccgacgg ccattaaaat agcaaggata cttgttgtta accaaagaaa
      721 aatagagtga cgttttatgt tcattggaag aaaggattaa agtttttttc gtggccaata
      781 gttgtattca tgccatggcc tgggtgaaca ctataatgac taggtaaggt gaatagttct
      841 tggcgaatac cagtaatgag gtcctcaaag ttactggtag gtaagtcagt acgaccgata
      901 gtttcacgaa atagagcatc gccagtaacg actaattcat ctgagtggaa aacaaatgag
      961 actcctcccc aagaatgacc aggtgttggt aaaactgtga attcaaaacc atttagttga
     1021 tagggttgct ttaatttaaa aaagttctct gccggtcttg ctataacatt aatgatatca
     1081 tcatgacggc ctaacccaga taaattatca tctggggaag atagccaagc ggcttctttt
     1141 tctgaaacat aaacaggtgg attatcaaaa gtatctctga ccaaatctaa actaaaaata
     1201 tgatcatagt gagtatgagt taagaggata gccactaaag gtttttcaaa ggatttaatt
     1261 ttagcaatga tttcttggcc attgctccct ggatcaatga ggataacagc ttgatcatta
     1321 acgaggtaat aagtattttc accagcaatg tggtttattg ttttatagat tttcataatg
     1381 atagtgtacc aaaaaatcaa gactttgtta aaaaaagagt ggcggaaaat aaatggcaac
     1441 tagtttcaga aaatatggga tctcacagca atttcttgac accgttttca taaaggtgta
     1501 tcataaaagt aatcaataag ccaagcctaa agctgaactt agtttaagaa cactgacttt
     1561 ttgagagcaa cctaattggt tgactaaaga atatgaggtg gaagatatgt caaaagttgc
     1621 tattgttact ggtgctggtc aaggaattgg atttgctatt gcgaaacgtc ttcatgcaga
     1681 cggctttaaa ataggcgttt tagactataa tgaagagact gctcaggctg cggttgataa
     1741 actatcacct gaggatgctg ttgcagttgt agcagatgtt tcaaaacgtg atcaagtctt
     1801 tgatgctttc caaaaagttg tagatacttt tggtgatttg aatgttgttg ttaacaatgc
     1861 aggtattgcc ccaacaaccc ctcttgacac tattactgaa gagcagtttg aaaaggcttt
     1921 tgcaatcaat gtcggaggaa ctatttgggg aagtcaagca gctcaaaaac attttagaga
     1981 attaggtcat ggcggaaaaa ttataaatgc aacttcacaa gcaggatgtg aagggaatcc
     2041 taatttgact gtttatggtg gtacaaaatt tgcagttcgt ggtattacac agactttggc
     2101 aaaggatttg gcatcagaag gaatcacagt caatgcctac gcaccaggta ttgttaaaac
     2161 acctatgatg tttgatattg tccatgaagt tggtaagaat gcaggcaaag atgatgaatg
     2221 gggtatggag cagtttgcaa aagatatcac tttgaaacgc ttgtctgaac cagaagatgt
     2281 tgctaatgca gtaggcttct tagcgggaga tgattcaaat tatatcactg gacaaacaat
     2341 agttgtcgat ggtggtatgg tattccatta aaaatgatta atgctaacac tgtgagtgaa
     2401 actcacagtg ttttttatat ttcttaaaaa attatcttat cttggttatt ggagtgatgg
     2461 catctttgag gtacagtcat gttttgtttt atagatatag cgtgctataa tagacttact
     2521 atgacccaga aaaaacttag aaaatatgca gtagttgact tggaggctac tggagcaggt
     2581 cccaatgctt ctattattca ggttgggatt gttattatcc aaggaaataa aattattgat
     2641 agttatgaaa cagatgttaa tcctcatgaa tctttggatg agcatattgt tcatttgaca
     2701 ggtattacgg ataagcagtt ggttaaagct ccggattttg gtcaagtggc gcatcacatt
     2761 tatcaattga ttgaagattg tatttttgtt gcccacaatg taaagttcga tgcaaattta
     2821 ttggctgagc aactattttt agaaggcttc gagttacgta caccaaggat agatacagta
     2881 gagttatcac aagtgtttta tccctgttta gaaaaatatt ctttaggggc attagcagaa
     2941 agcttaaata ttgaattaac agatgcgcac actgctattg cagatgcaag agctacagca
     3001 cagttattca ttaagttaaa agctaaaatt agtagtttac ctaaagaagt gttggagacc
     3061 attttaacat ttgctgataa tcttttgttc gaatcctatt tattgattga agaagcttat
     3121 caagaagctg atttcgttaa tcctaaagaa tattatttct ggcaaggatt ggtcttaaaa
     3181 aaagaaaaag ctgttggaaa gcctaagaaa ttatctagtg attttcaagt taacatggct
     3241 ttgttaggta tggatgctcg ccctaagcaa attgtttttg ctgacttggt taaagcgcat
     3301 tttaatgatc aaacaacaac gtttttagaa gctcaatcag gacttggtaa aacatatgga
     3361 tatctcttgc ctttattgga tcagtctcaa aaacaacaaa taatagtatc tgttccaaca
     3421 aagattttgc aagatcaaat aatggctaaa gaaataaagc acattcaaga actttttcat
     3481 attccctgtc acagtatcaa aggaccgaga aattacttga agttagatgc gttttataag
     3541 agcctccaag tgcaggatcg aaatcgattg ataaatcgtt ttaaaatgca actgctagtg
     3601 tggttgacgg aaacgactac aggtgatttg gatgaaatta aacaaaaaca acgattagaa
     3661 tcttactttg accagctaaa acacgatgga gaagtgactc aatcatcact tttttatgat
     3721 ctggattttt ggaaacgcag ttatgataaa gttgcacaaa gtcaacttgt tattattaat
     3781 catgcttatt ttcttgagcg tgttcaggat gacaaagatt ttgcaaaggg gaaggtttta
     3841 gtttttgatg aagctcagaa acttgttttg ggactggaaa atttttctcg tgggcaactt
     3901 gatattagtc accaattgca ggttattcaa aaaataattg atagtcctat tccgttatta
     3961 caaaagcgcc ttctggaaag tatttcttat gagttaagtc atgcagcaga acttttttat
     4021 cgtcataatt cttttgaatt ttcagagacc tggttgaaac gtctaaaaaa tagtataaac
     4081 gctttagaag tagtaggatt agatgattta caaacttttt tcacagctac ttatactaac
     4141 tattggtttg aaactgataa agtcaatgag aagcgtttaa ccattttacg tggggcgaga
     4201 gaagattttc ttaaattttc agagttcctc cctcccacaa aaaagaccta tatgatatca
     4261 gctactttac aaatcagtcc caaagtctac ttgtcagatc ttttaggagg attttcaagt
     4321 ataagcacag agaagatagc gcatgagaaa aatgccaatc agaaagtatg gattgatacc
     4381 tctatgccta atatacttga tttatcaccg gaacagtatg cttacgaaat agccaagcgt
     4441 ttacaagata taatgacgtt aaagcagcct accttagtct tattaacttc taaacagaca
     4501 atgtttatgg tatctgatta tttggataag tgggagataa aacatcttac tcaagataaa
     4561 aatggcttag cttataacgt taaaaaacgt tttgatcgtg gagaaagcaa tcttctttta
     4621 ggtacaggat ctttttggga aggtgttgac tttgtacatc gtgatcgtct tattgaagtt
     4681 atcacgcgat tgccttttga taatcccaaa gactatttta tccagaaact atcgcagagt
     4741 ttaacaaaag aggggaaaaa cttcttttat gactattctt taccaatgac tgtcttaaaa
     4801 ttaaaacagg ccttgggacg tactacaagg agagaagaac aaaagtcagc ggtaattatt
     4861 ttggatagca gattggttat taaatcttat ggacaaacca tcatgcattc tcttggtaga
     4921 gattttgaaa tttctaagga aaaaattaac aaggtcttga ctgaaatggc taaatttttg
     4981 atataatggt aaagatcatt gtctaaaaat aagatgttga cagtgcgact caagattaaa
     5041 taatagtagt acgttgataa aaggaggagt gatgacctat ttgtcagaac gtgtactcaa
     5101 tatggaagag agtgttactt tagctgcggg agctaaagca agagaactca gagtacaagg
     5161 aagagatatt cttagtttaa ctttagggga gcctgatttc gctactccta aaaacattca
     5221 gcaagcggct attgaggcta taacagatgg acgtgcaagt ttttacacac catcaagtgg
     5281 tttgccggag ttgaagtcag caattaatgc gtattttgaa cgtttttatg gctactccct
     5341 taaacctaat caggtggttg tgggaactgg tgctaagttt atcctttata ctttctttat
     5401 gacagtactg aatcccggtg atgaagtaat tattccgact ccttattggg tatcttatgc
     5461 tgatcagatt aagatggctg aaggtaagcc agtttttgtc acagcaaaag aagttaatca
     5521 cttcaaagta actgtggaac agttagaagc tgttaggact gataagacaa aagtcatact
     5581 tcttaatagt ccatcaaatc ctacaggtat gatttataaa gctgaggagt tggaggctat
     5641 tggaaattgg gcagtcgaac atgatatcct aattttagca gatgatattt acggtcgatt
     5701 ggtttacaat ggcaatattt ttacccctat ttcaagcctt tcagagtcca ttcgaaacca
     5761 aacgatagtc ataaatggag tttctaagac ttatgcgatg acaggttggc gtgttggttt
     5821 tgcagtgggt aatcatgata ttatagcggc aatgtctaaa gtagtcagtc aaacaacttc
     5881 taaccttacg gctgtctcac agtatgctac tattgaggct ttaaatggtt ctcaagagtc
     5941 atttgaaaaa atgaggctag catttgaaga acgccttaat atcatttatc cattgttgtg
     6001 tcaggtccct gggtttgagg ttgttaaacc acaaggtgct ttttatcttt tcccaaatgt
     6061 tacaaaagca atggaaatga aaggatatac ggatgttaca gcattcacgg atgccattct
     6121 tgaagaagtt ggacttgctt tagtaacggg tgctggtttt ggagcgccgg aaaatgttcg
     6181 tctgagttat gctacagatc ttggaaccct taaagaagct gttcgtagac tgcatgtctt
     6241 tatgggaagt aatgaaatta attaaataaa aatagagaga aacttatgtc aaaaaaactt
     6301 atttcaattg ttgatgttaa agattatgtt ggtcaagaag ttactattgg tgcttgggtt
     6361 gctaacaaat caggtaaagg taagattgcg tttgtacagt tgcgcgatgg ttctgccttt
     6421 ttccaaggtg tcgcttttaa accaaacttc attgaaaaat atggtgaaga atcaggtctt
     6481 gaaaaatttg acgttattaa acgtctcaac caagaaacat ctgtttatgt aacagggatt
     6541 gttaaagagg atgaacgttc aaaatttggc tatgagcttg atattactga ccttgaagtc
     6601 attggagagt cacatgaata tcctataact cctaaagagc atgggacaga cttcttgatg
     6661 gacaaccgtc atctttggtt gcgttctcgt aaacaaatgg ctgttatgca aattcgtaac
     6721 gctattattt attcaactta tgaattcttt gaccaaaatg gttttatcaa atttgatagc
     6781 ccaattttat cagaaaatgc agcagaagat tcaacagagt tattcgaaac agactatttt
     6841 gggaaaccag catttcttag ccaatctggt caattatacc ttgaagctgg agcaatggct
     6901 ctaggccgag ttttcgactt tggtccagtc tttcgtgctg aaaaatctaa aacacgtcgc
     6961 catttaacag aattttggat gatggatgcc gagtattctt tcttgtcaca tgaggaatca
     7021 cttgaccttc aagaagctta tgttaaagca ttgatccaag gcgttctaga tcgtgcgcct
     7081 caagcacttg atattttaga acgtgatgtt gaggctctca aacgttatat cgcagagcca
     7141 ttcaagcgtg tttcttatga tgatgctatt acacttcttc aagagcatga agcagatgaa
     7201 gatacagatt atgaacattt agagcatggt gatgactttg gttcaccaca tgaaacttgg
     7261 atttctaatt actttggagt gccgacattt gtagtgaatt acccagcaag ctttaaagca
     7321 ttttatatga aaccagttcc tggaaatcca gaacgcgttc tttgtgctga tcttttagct
     7381 ccagaaggtt atggagagat cattggcggc tcaatgcgtg aagacaacta cgatgctctt
     7441 gtggctaaaa tggatgaact aggtatggat aaatcagaat acgactttta ccttgacctt
     7501 cgtaaatacg gctctgttcc tcatggcggt tttggtatag gtattgaacg tatggtaact
     7561 tttgttgctg gtaccaaaca tattcgcgaa gctattccat tcccacgtat gttacatcgt
     7621 atcaaaccat aataaaaaag cgctagctag tgcgcttttt tattatggtc tttagaatcg
     7681 ttggttaatt aaattgggac tatgtttttt taacaatgtg attgtcgaaa agacaaggat
     7741 aagactgatt agtcgatcaa tataatctgt tccaacttga acaagtatca aactacttgt
     7801 catgtctaat ccaattccat gtaagaattg tgctataata cttgagccac tagaggtaat
     7861 tcctttaaaa aggataactg ttataactga agcaataata gttcctggta aagaaataat
     7921 cagtgatttc cacaataggc tagagggttt acaattcctt ttaatcaaga taccagtaat
     7981 gatagcaact acgatggcga ctggtgaata ataaagtgaa aaaatatcag tagtcatcca
     8041 attgatgagt gcactgagaa aagcagtgct ggcagcgtac catggtccca ggataactgc
     8101 tattagcaat gtacctatag tatctagata gataggtagg cgtaaaaata gagctaaatt
     8161 agccccaata aggttgatgg cgatagcgat tgagataaat gaaattaaac gagcaggtga
     8221 ttttgtcatt tgttaagtcc taacatttct aagtcttcaa tgatgactga attttgtgca
     8281 tgtaatagga cggtcataaa atcattccaa aataaattag tattgactga ggttaaaatc
     8341 ttactgttag catctttttt ataaaaatga tactgatcta caattgtttg tcccatggcg
     8401 attccgtgac aagcaacatc ggtatagcta tcaaaaccag tagcgatatt ttcattaaca
     8461 aagtaagcaa tagctaaagg atcatttatc acacatccaa taatatgctc atattgccag
     8521 tgaaaatcga agtagaactt tgtgattttt tggataaaag ttgatacgtc tggattaata
     8581 cgctccatat aagataaatg attgggggtt aatacaatat gacgtgtgat gtctagtcca
     8641 accatctcta tttttttgtc aaggttttca aatacatatt gtgcagcatg gggatcatac
     8701 caatagttgt attcagcaac tggagaacaa ttgccatgtg atttaaaatt tcctcccatg
     8761 ctaacaaatc gcttgcaatg tttgcctaat ttgggattag tttgtaaggc tcgagcgata
     8821 ttggtcagag gtccgagagc tataatagaa gtatcatttt tgtgttcaaa atagtttgct
     8881 aaaaaacaat ctgctgattc ttcttgaaag ataattggtt gcgccaatgt aaagttattc
     8941 tctccaaggc catccattcc atgagtatct tgagcagata caaattcacg ttgtaaaggc
     9001 aagttattac caacataaac tgaaatgtcg cgacgattta gtaattcaag cgttacaaag
     9061 gtattcttta gtcctagctc aacaggcgaa ttgcctgctg ttatagtaat agcaacaact
     9121 tctaacttag gatgctgaat agcatacatc agtgctaagg tatcatcaat accgggatcg
     9181 caatcgataa taattttttc tttgttcatt tgtctccttt cttttaatat tagtgaatgt
     9241 tctatgcttt tatgaaacta ttaacaatag tcatggcaat aaaaaagatc ttggcagtta
     9301 ccaagatcac acgaaaaaat gaatttagtt ttctaaattc aatcatattt tcagtcactt
     9361 agttttttat tctgggaagt tttcgaacca gtccgagaaa tcctcgatta tgcacgtgtt
     9421 ttattataat atgaaagcac taacttgtaa agattcaaac tgaccattta acagtgtcta
     9481 gattgattgt tttagcattt gaaattaatt ttgaaatcgg acatctgctg tgtgctttgt
     9541 taacgattag cttactatca gataatgaca gtttttcaat actagcaaca gcgttaactt
     9601 ggaaataaaa tcccttacct acttgctttt ccttcatgag ctggcaatta acatcaacac
     9661 gagattttag gtctttgaat cctttttgtt ctaaaatagc ttttatagtt gcatttaaac
     9721 atgttgacca tgctaaagca attaattctt caggattaaa tcctggtaaa tgatttaaag
     9781 ggtgattggt ctccacaatt tttccattgg agagataaac ttttccatca acgccttgag
     9841 tgtttgatga ggttacttca taaagcataa taaactcctt tgggtctagt ttaccaaatt
     9901 tgggagcatt ccgacaagat tttactattt agaatgagtt tcttttcctc tgaagtaaaa
     9961 tcctaattgc tagccatttg tggtataatg gcataaaaca taagagaaag aggtaatcgt
    10021 aatgtcagac gaacaaatca aacttgtgat tgtaacaggt atgagtgggg caggaaagac
    10081 agtggctatt cagtcatttg aagatttagg atattttaca atagataata tgccgcctac
    10141 tctagtgcct aagtttttag aattagcagc acaatcagga gatacaagca agattgctat
    10201 ggtagttgac atgcgtagtc gtcttttctt tagagaaatt aactctattt tggatagcct
    10261 tgaaattaat gataatatta actttaagat tctattttta gatgctactg acaccgagct
    10321 agtgtcacga tataaagaga cacgtcgtag ccaccctctt gctgccgacg gcagagtgtt
    10381 agatggtatt tctctagaga gagaactatt agcaccgctt aaaagtatga gtcagaacgt
    10441 tgtggacact agtgaattaa caccaagaca actacgtaag gtcatttctg aagaattctc
    10501 aaatcaagat agtcaaagtt cttttcgtat tgaagttatg agttttgggt ttaaatatgg
    10561 tatacctttg gatgcggatt tagtattcga tgtgcgtttt ttaccaaatc catactataa
    10621 accagaatta agagataaga caggtttaga tactgaggtt tatgactatg tcatgagttt
    10681 tgatgaatca gacgattttt atgaccatct tcttgcctta ataaagccaa tcttaccagg
    10741 ctatcaaaaa gaagggaaat ctgttttgac tgtggcaatc ggatgtacag gtgggcaaca
    10801 tcgtagcacc gcttttgctc atagattatc agaagattta aaagcagatt ggacagtgaa
    10861 tgaaagccat cgtgataaaa ataagcgtaa ggagacggtg aatcgttcat gagaaaacct
    10921 aaaataaccg ttattggcgg aggaacggga attccagtta ttttgaaaag tcttcgctta
    10981 gaagatgttg agattacagc ggttgtcacg gtggcagatg atggtggttc gtcaggtgag
    11041 ttgcgctcag ttatgcaact aacaccgcca ggagatttaa gaaatgtttt ggttgccttg
    11101 agtgatatgc cgaagtttta cgaacaaatt ttccaatatc gttttgcaga aggtgatggg
    11161 gattttgcag gtcatccttt aggaaatctt attattgctg gtgttgctga aatgcaaggt
    11221 tcaacttata acgctatgca gagtttaaca cagttctttc atacaacagg aaagatatat
    11281 ccatctagtg aacatccgct cactctgcat gctgttttta aagatggcca tgaagtggtt
    11341 ggagagagtc aaatagccga ttataaaggc atgattgacc atgtgtatgt cactaatact
    11401 tataatgaag agactcctac ggccagccgc aaagttgttg atgcaatttt agaaagtgat
    11461 atgattgtgt tgggtccagg gtcacttttc acctctattt tacctaacct agttattcca
    11521 gagattaaac aagctctttt agaaactcgg gcagaggtag cttatgtatg caatattatg
    11581 acacaacgtg gtgaaactga gcattttaca gacgcagacc acgttgaagt attgaaacgt
    11641 catttaggac aagatgctat cgatacagtt ttggttaata ttgaaaaagt gccagagtct
    11701 tatatggaaa acaatcattt tgatgagtat ttggtacagg ttgaacatga tttttcaggg
    11761 cttagaaaac atgccagacg tgtcatctcg tctaactttt taaaattaga aaaaggtgga
    11821 gccttccatc atggtgactt cgttgttgag gaactaatga atttagtagg aacacattta
    11881 tgagttttac agtaaaagtc aaagaagagt tgctaggtca taagagtgaa aataagatgg
    11941 aactgtcagc tattatcaaa atgtcaggta gtttaggatt agctaaccac ggcctcaacc
    12001 tttctattac cacagaaaat gctaaaattg ctcgtcatat ttatagcatg ttagaagaac
    12061 attatcattt gcagccagaa atcaaatacc atcaaaaaac gaacttacga aagaatcgtg
    12121 tgtatactgt ttttattgaa gaaaaagttg atgttattct tgctgatttg aagttggctg
    12181 atgctttttt tggtattgaa actggtattg aacactctat attggacaat gatgaaaatg
    12241 gaagagctta tctcagggga gcattcttat caacgggtac tgttagagaa cctgattctg
    12301 gaaaatatca actagaaatt ttttctgttt atctagacca tgcacaagac ttagctaatc
    12361 ttatgaaaaa atttatgtta gatgcaaagg ttattgaaca taaacatgga gcagtaactt
    12421 atttgcaaaa agcagaagat attatggatt tcttgatagt cattgatgcc atggaagcta
    12481 gagatgcttt tgaagagatt aaaatgatac gtgagacacg taatgatatt aaccgtgcta
    12541 acaacgttga aacagctaat attgctagaa caatcacagc tagtatgaag actatcaata
    12601 atattatcaa gattatggat actattggtt ttgatgcact gcctagtgat ttaagacaag
    12661 tagctcaggt gcgtgtggct catccagatt actctattca acaaatagca gacagtttag
    12721 aaacgcctct gagtaagagt ggcgttaacc atcgcttacg gaaaataaat aaaattgcag
    12781 atgaattata gaaggagata ttagcatggc ttgtacaaca atattggttg gtaaaaaggc
    12841 ttcttatgat ggttcgacta tgatcgctag aacggaagac tctgttaatg gcgatttcac
    12901 acccaaaaaa ttaaaggtaa tgacatctaa agatcaaccg cgtcattaca aatcagtttt
    12961 atcaaatttt gaagtagatt taccagataa cccacttcct tatacttcag taccggacgc
    13021 attgggaaaa gatggtatat ggggtgaagc cggtattaac agtaaaaatg tagcgatgag
    13081 tgctacagaa actattacaa cgaattcccg cgttttgggt gcagatcctt tggtttcaga
    13141 tggtataggg gaagaggata tactcacttt agtgcttccc tatattcagt cagcgcgaga
    13201 aggtgtggag cgtttaggtg ctattttgga aaaatatgga acctatgaat caaatggtat
    13261 tgctttttca gataccgaag aaatatggtg gttagaaaca attggtgggc atcattggat
    13321 tgctcgtcgc gtacctgatg atgtttatgt tactaatcct aaccaactag gaattgatca
    13381 ttttgaattt aataactgtg atgactacat gtgctctagt gatttgaaag agtttatcga
    13441 acaataccat ttagatttga cctattctaa tgagcatttc aatcctcgat atgcttttgg
    13501 tagccaacgt gataaagatc gtcattacaa cacaccaaga agttgggcaa tgcagcgttt
    13561 tttaaatcct gaaattgaac aggatccacg tagtttgttt attccctggt gtcaaaagcc
    13621 ttaccgaaaa attactgttg aggatattaa atatgtgttg agtgatcatt atcaagacag
    13681 tgtgtatgac ccatatggac cagaagggga tgcggtaagt aggagagctt ttcgttcagt
    13741 tggtatcaac cgaactagtc aaacgtctat tctacaattg cgaccaaata aatcacttga
    13801 aacgacaggt gttcaatggt tatcttatgg ctctatgcca tttgcaacca tggtgccgtt
    13861 gtttacacaa gttgagactg taccagacta tttttcgaat acaaccaagg atgcttcaac
    13921 agataatttt tattggacca atcgtttaat tgcagctcta gcagatccac acttttatca
    13981 acatgaagct gatattgaaa gctatatcga gagaacgatg gcccaaggac atgcacatat
    14041 taatggtatt gatagagaag ttgctgagaa taaagagatt gattttcaac agaaaaatca
    14101 agaaatgagt gactatattc aaaaagaaag ccaagaattg ttaaatcgta ttctatttga
    14161 tgcaagtaat ttaatgacaa atcgcttttc aatgggagat taaaaaactc ggaatatttt
    14221 ccgggttttt taagataaaa acgcttgcat aagtatataa aacttgcaaa aaaaaaatat
    14281 cgtgctatac tttactagta aaatattaac cggtaaataa cgggttaaat aaaaaggaga
    14341 aaaaatgaga aagaaatttc ttttattgat aagctttgta gctatgtttg cagcttggca
    14401 acttgttcaa gttaaacaag tttgggctga tagtaaactt aaagtggtaa caacttttta
    14461 cccagtttat gagtttacaa aaaatgtcgt tggtgataaa gctgatgtat ctatgttaat
    14521 taaagcaggt acagagccgc atgattttga accatcaact aaaaacatcg ctgccatcca
    14581 agattcaaat gcttttgttt acatggatga taacatggaa acttgggctc caaaagtagc
    14641 taagtcagtt aaatccaaaa aagtaacaac tattaaaggt actggcgata tgttacttac
    14701 taaaggcgtc gaagaagaag gtgaagaaca tgaaggacat ggtcatgaag ggcatcatca
    14761 tgaacttgac ccacacgtat ggttgtctcc agaacgtgcg atttctgttg tagaaaacat
    14821 ccgtaataaa tttgtcaaag cttatccaaa agatgcagct tcatttaata aaaatgcaga
    14881 tgcttacatt gcaaaattaa aagagcttga caaagaatac aaaaatggtt tgtcaaatgc
    14941 taaacaaaag agttttgtga ctcaacacgc agcgtttggt tacatggcgc ttgattacgg
    15001 tttaaatcaa gttccaattg ctggtcttac tccagatgca gaaccttcat caaaacgttt
    15061 aggcgaatta gctaaataca tcaagaaata taacatcaac tacatttatt ttgaagaaaa
    15121 tgcttcaaat aaagttgcta aaactttagc agatgaagtt ggcgtgaaaa cagctgtgct
    15181 tagtccactt gaaggacttt ctaaaaaaga aatggcagct ggcgaagatt acttctcagt
    15241 tatgagacgt aatttgaaag ttcttaaaaa gacaacagat gttgcaggta aagaagtagc
    15301 tcctgaagaa gataaaacta aaacagttga aacaggttac tttaaaacta aagatgttaa
    15361 agaccgtaaa ttgacagatt actctggtaa ttggcaatca gtatatcctc ttcttcaaga
    15421 tggaacactt gatccagttt gggattacaa agctaaatct aaaaaagata tgactgctgc
    15481 agagtacaaa aaatattata cagcaggtta caagactgac gtagaatcaa tcaagattga
    15541 tggtaaaaaa caccaaatga cctttgtacg taatggtaaa tcacaaacat ttacatacaa
    15601 atatgcaggt tacaaaatct taacttataa aaaaggtaat cgtggagtac gttatctctt
    15661 tgaagctaag gaaaaagatg ctggtcaatt caaatatatc caatttagtg accatggtat
    15721 taaaccgaat aaagctgaac acttccatat cttctggggt tcagaaagcc aagaaaaatt
    15781 atttgaggaa atggaaaact ggccaacata cttcccagct aaaatgtctg gacgtgaagt
    15841 tgcccaagac cttatgtctc attaatattt aaaagatgat atcggatacg atatcatctt
    15901 tttttcttcg tacgtgatag gctcaaacat agaaaaaagc acttctatta gaagtgcttt
    15961 taaaattaat gctatgctta ttgagcagcg tttgcgtctt tgagaccata ttttttgttg
    16021 aaacggtcca cacgtccatc tgcttgagtg aatttttgac gtcctgtata gaatgggtgt
    16081 gaatctgatg agatttccac acggataagt gggtaagttt caccttcgaa ttctactgtt
    16141 tctttagttg atttagttga accactaagg aatttgtaac ctgtagttgt atctaagaaa
    16201 acaactggac gataatctgg atggatgtct tttttcatct ttaaaaattt cctttctgcc
    16261 ataactcgtc tgagtcatag ttattaacca ctctattcta tcaaataaga atagccttga
    16321 caagtgtttt gataaaaaaa gtaaaattat aaagaaagta cttcctggat ttcttggtaa
    16381 atagcttgat tctcttccaa actataagaa ttagctccac tggctaaagg atggccgcct
    16441 ccattatgac gtttagctat ttcattaatg atgtgtgact tactacgtag gcgcacacga
    16501 taatgcccat cgctttgttc aacgaaaatt gcccaacttt ctacaatatc aatttttcca
    16561 ggtataccaa cgattgcagc agtctctgca tctgttacat caaagcgttt taagtcttct
    16621 tgtgttaaag taacgcatgc agcgccgttc ttgtcaattt caagttgctc aaaaataaag
    16681 ccttgtaatt ttgcaatttt gaaaggaaag gaatccattt gacgtgccat tgcagaaaaa
    16741 tcaaagtcaa attcacgaag tttgctagca atcttcagtg ttttacttgt ggtagcagga
    16801 tataaaaagc gtcctgtatc tcctacaata ccattatata gaactcttgc tgcacttgtt
    16861 gataacaaca aatcacaaga aagagcaaag tctgtcacaa tttcacttgc actagaggcg
    16921 ttagtatcta cataagagag gtctccatag acttcatcgt ttggatgatg gtcaatttta
    16981 attaaaaagt cacctttttt ataacgttca tcatcaatgc gaggggtatt tgctgtatct
    17041 gtaacaataa caagagcgcc ttgatagtct tggtcggtca cttgatccat tttagcaatc
    17101 caagcgagtg tgggttcgtc aaaaccagta gcaagtactt ttttctttgg gaaattatgc
    17161 ctgatgatat cccttaagcc gatttggcta ccaagagcat cagggtctgg tctcatatgt
    17221 cgatggataa tgatggtgtc gtactctttg attttatcaa gaatttgttg aaaaataatc
    17281 attttttacc tacctaactg ttttgtcttt tagtgaagaa caaggctata ttgttgacta
    17341 tattatagct taaatctttt attgaggaaa gaaattaaga tagcggccgc aatgtttgtt
    17401 tatttttata tgatggtatg ttattttttt tataaaattc ggatactgct aactaacaat
    17461 cttttttatg ataatatttt gtaaatgtat tgctttttag ctaacttttg ttaatatagt
    17521 aaaaatgctt tttaatagga gataaattat gacacaagca gtattaaaag aattggctaa
    17581 ggcggagttg cattgtcatc tagatggctc actttcttta ccagctatta gaaaattggc
    17641 taatatggct gatataatac tgccaaatag tgataaagaa ttaagaaaat atgttatagc
    17701 tccagctcaa acagaaagct tggtagatta ccttaaaacg tttgagttta tcagaccgct
    17761 tcttcaaacg aaggaagcat tacgttttgc tgcttatgat gttgctagac aggcagcttt
    17821 agaaaatgtt atttatattg aaattcgttt tgcgccagaa ctctctatgg ataaaggttt
    17881 aaccgcaagt gatacagtct ttgcagtctt agaaggttta gcagatgctc agaaagaatt
    17941 taatatcgtt gcacgcgccc ttgtctgcgg tatgcgccaa tcgtctcata aaacgaccaa
    18001 ggaaattatt aaacacattg ttgatttggc tcccaaaggc ctggtaggtt ttgattttgc
    18061 aggtgatgaa ttttcctatc caacagatag tttagtagat cttattcagg aagtaaaacg
    18121 ctcaggttac ccgatgactt tacatgcagg tgagtgtggt tgtgctaagc atattgcgga
    18181 tagtttaaat ttagggatta agcgtatggg acatgtgaca gctttaacag gacagagggc
    18241 tttaataaag cgatttgtcg aagaagatgc tgtagcagag atgtgtttaa ctagcaattt
    18301 acaaacgaaa gcagcctcaa gtattcaaag ttttccttat caagaacttt atgatgctgg
    18361 aggtaagatt acaattaata cagataatcg tactgtttcc gatacaaatt tgacgaaaga
    18421 atatagtctt ttcgtaactt attttggaac gaaaatagaa gactttcttg tttttaatca
    18481 aaacgctgtg aaggcctcct ttacaagtga ttcagagaaa gatactttac tacataagct
    18541 acaagaaaac tatgacagct atctaaaata accttcacga ttctttaaaa atatgataga
    18601 atagtggtaa tttacaagtg gaggaagata tggctttagc aaagattgtt tatgctagca
    18661 tgaccggaaa tactgaagaa attgctgaca ttgttgctga taaattacgt gacttgggtc
    18721 tagatgttga agtagaagag tgtacgatgg ttgatgctgc agattttgaa gatgctgaca
    18781 ttgcaattgt tgccacttat acatatggtg atggagattt accagatgaa atcgtggact
    18841 tttatgaaga tttagcagaa gttgatttat ctggtaaagt ttacggcgta gtgggttcag
    18901 gtgatacttt ctatgattac ttctgcaaat ctgttgatga gttcgaggct caattcgcat
    18961 taacaggagc acaaaaaggt gctgattgtg ttaaagttga tttagcggca gaagacgaag
    19021 acattgaaaa cttagaagct tttgctgaag aaattgcttc taaacttaac taagctacca
    19081 aaaagacgaa cacgagtcgt ctttttaact tttttaccat cttttgtaac tagaatatga
    19141 gatatagata tggaattaga agagattcgt caagaaatag atgaaataga tcagcaattg
    19201 gtaagccttt tagaaactag aatgggtcta attttagagg tcatagcatt taaaaagaaa
    19261 caccgacttc cagttttaga taataatcgt gaaaacgaag tgcttaacaa tgtattgaag
    19321 aaggtccaaa atcatcaatt tgacgatgtg atacgagcaa ctttcaaaga cattatgaca
    19381 gaatctcgtg tttatcaaaa ggagaatatt gtagacggtg attaaacgat ttgataatta
    19441 tgctcagtac gtacttgttt tagggtttag cttactaatc ggtggtgtgg ttggggctat
    19501 tacagcagtc tttggacgtg ttctattatt cctgacagcc tttagaagtg attatatagc
    19561 ttacctactc ccttttctta gcattgttgg tttatttata gtttttgtct atcaaaaatt
    19621 tgggggtaag agtgttaaag ggatgggatt ggtatttgaa gtaggtcatg gtaatgagga
    19681 gactattccg aaacgtcttg ttcctctcgt gatattaaca acttggttaa cccacttatt
    19741 tggtggtagt gcaggtcgtg agggggttgc tgtacagatt ggggctaccg tttcacacta
    19801 ctttcaaaaa tattgccgtt tacaaaacgc ttctcaactt ttcctagtaa tgggaatggc
    19861 agcaggtttt gctggtttat ttcagactcc tttagctgct actttttttg cgatcgaagt
    19921 tttggtagta ggtcgtttaa tggtttcgta tatattacca agtttaattg ctgctctgac
    19981 agcaaatttc gtctcacatt ctttaggttt agaaaaattt tctcatagta tagcaacatc
    20041 aatggcgtta acaccagata ttattctaaa attattggtt ttaggactat gttttggtct
    20101 gtgtggcaat ttatttgctt atttattggc taaagctaaa ttaattgctt ctagtaggtt
    20161 actaaaccca tataaacgga ttttcacact tggattgtta gctacttttt tattgtttat
    20221 cttccatttt ggacgatatt caggactggg aacaaatcta attgaggcta gcttcactaa
    20281 taagaatctt tatgattatg attggttgtt aaaattgtgt ttgacagtta ttactttggc
    20341 tgcaggttat cagggaggag aagtaactcc cttatttgca ataggagcaa gtttaggagt
    20401 gattattgcg cccattttag gtttgccagt tattttagtt gctgctttag gctatactag
    20461 cgtttttggg agtgcaacga atactctgtt aggtcctatc ttaataggtg gcgaagtctt
    20521 tggctttgcc aacactcctt attttgttat tgtatgtctt gttgcttact ccatatcaca
    20581 tgctcatacg atttatggtg ctcaatctag atagttagtg atgagtttta aaagagttta
    20641 tcagtttata gaacggaata ttccgttctc ttttttattc tcttaaagtt tataaaaatg
    20701 aagtttgtaa attt
//
LOCUS       DATRQA010000019        20508 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.019, whole genome shotgun sequence.
ACCESSION   DATRQA010000019 DATRQA010000000
VERSION     DATRQA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20508
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.019"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..1582
                     /gene="fbsB"
                     /locus_tag="VBD27_000522"
     CDS             <1..1582
                     /gene="fbsB"
                     /locus_tag="VBD27_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibrinogen-binding surface protein FbsB"
                     /protein_id="HEO7024557.1"
                     /translation="TKLTVNQYEAIVNRMSVALQKFSKNIFEKQKVNKDLIPIAFWIE
                     KSYRTVGTNEIAASVGIQGGFYQNFHDYYNYSYLLNSLWYEGNVKEVVKDYENTIRQI
                     LSKKHEIEKILNQSTSDISIDDDDYEKGNKELLREKLNIILNLSKRDYRVTPYYEVNK
                     LHTGLILLEDVPNLKIAKDKLFSLENSLKEYKGEKVNYEELRFNTEPLTSYLENKEKF
                     LVPNIPYKNKLILREEDKYSFEDDEEEFGNELLSYNKLKNEVLPVNITTSTILKPFEQ
                     KKIVEDFNPYSNLDNLEIKKIRLNGSQKQKVEQEKTKSPTPQKEAVKEQTEQKISGKT
                     QNVEKKSETVATPQQSSTAQTSVQQPAPVQPVVQESKASQEEINAAHDAISAYKSTVN
                     IANTAGVTTAEMTSLINTQTSNLSDVEKALGNNKVNNGAVNVLREDTSRLENMIWNRA
                     YQAIEEFNVARNTYNNQMKSETVPVDNDIEAILAGSQAKISHLDNRIGARHMDQAFVA
                     SLLEVTEMSKSISSRIKE"
     gene            1649..2194
                     /locus_tag="VBD27_000523"
     CDS             1649..2194
                     /locus_tag="VBD27_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024558.1"
                     /translation="MKKITTLILASSLLLVATTSVKADDNFEVPTRYVKMSEKSKAFY
                     QRLQEKQRKTHTTVKTFNNSEIRHQLPLKQEKARNDIYNLGILISQESKGFIQRIDNA
                     YSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQGNTDLKNFYAELDDMY
                     EHVYLNESRVEAINRNIQKYN"
     regulatory      2345..2434
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2590..3156
                     /gene="thiW"
                     /locus_tag="VBD27_000524"
     CDS             2590..3156
                     /gene="thiW"
                     /locus_tag="VBD27_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEO7024559.1"
                     /translation="MTNHKTKKLTVLAIMIALDVFLTPIFRIEGMAFMSSVVNIIAGV
                     IMGPIYALVMALVTAFIRILTQAGVASVAPLAILGAGPGAFLAGWFYRFYAKDWMSWV
                     GEFIGTGIIGALLSAPIMVWFWTWTADGNHEVLAKASAAQSIFLFMPRFIGATIIGGV
                     IALLLLQGLKRVPVFMTIQALFKGEKVK"
     gene            3153..3707
                     /locus_tag="VBD27_000525"
     CDS             3153..3707
                     /locus_tag="VBD27_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7024560.1"
                     /translation="MKLGLKDVLLIALLAVVLGVVYFGAGYISNAFVPFVGPIAHEVI
                     YGIWFVAGPMALYILRKPGTAIVAELLAALIEVLIGSIYGPSVLVIGTLQGLGSELGF
                     TLFRYHNYKLPAFILSAILTSIFSFAWSFYANGLSAFSFSYNILMLIVRTVSSIIFFL
                     LTKNICDQLHRSGVLNAYGIHEGK"
     gene            3711..4997
                     /locus_tag="VBD27_000526"
     CDS             3711..4997
                     /locus_tag="VBD27_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7024561.1"
                     /translation="MLSVEKLAYTHGDSHYLFDEVTFSLNPGERILISGYSGCGKSTL
                     ALLLSGLKESGKGQVLLNGSLIEPSDVGFLFQNPDLQFCMDTVAHELYFILENLQIEP
                     EQMQDRSEFVLAQVGLKGFQNRLIYTLSQGEKQRLALATIFLKSPKLIILDEAFANLD
                     QESASQLLQLVLNYQANNQSMLIVIDHLITYYQDIMDHYFWLEKWLIRVDFDYMLNRL
                     NVFELEKKSHNTGDKLLSIKDFQVKLSKNKFISYLDFDLASGERLCLDGPSGVGKSSF
                     FMGLLGLYRTKGKKQFTHRKQIPISFLFQNPLDQFIFSTVYDEIFQVCKDSNKARDIL
                     ETINLWDKKQFSPFQLSQGQQRRLAIGSILASDSKLLLLDEPTYGQDAYHANMITTLL
                     LSYCHKNHCGVIFTSHDPHLKECFATRFLEVSSCSI"
     gene            4985..5653
                     /locus_tag="VBD27_000527"
     CDS             4985..5653
                     /locus_tag="VBD27_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEO7024562.1"
                     /translation="MFNLKNYHPAITFLCLLIPTFSFSFTLRLRTSLLFLIIVVTLQC
                     FVKVSLKTWAKVNLISFVMGLSLFLGTYFWGKLPHQFVLASLVACRPLIFMNVGLLFH
                     ASHSNYDFIESLYQTFKVPSHFAYGIFAVFNLLPLIKLQYQRNRLAFRLKNQVTWALS
                     PRLILSVLLKTIYWVEQLELAMLSKGFEVGKERTHASTYPVRFRDYSLLGMSILLAIG
                     MIFK"
     gene            5737..6417
                     /gene="tenA"
                     /locus_tag="VBD27_000528"
     CDS             5737..6417
                     /gene="tenA"
                     /locus_tag="VBD27_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEO7024563.1"
                     /translation="MLKDEMIAKGTKYIQSIYQDPFIQGIVKGRLDHDVICHYLQADN
                     IYLGKFADIYALCLAKSDNLRDKQFFLEQVDFTLNRELADGEGPHQALAAYTNRSYQD
                     IIEKAVWYPSADHYIKHMYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGN
                     PFNNWITFYANDTVEELMENYFRMMDYYAQNLSKEKQADLVDAFVKSCQHERRFFQMA
                     INQEKWED"
     gene            6419..7216
                     /gene="thiD"
                     /locus_tag="VBD27_000529"
     CDS             6419..7216
                     /gene="thiD"
                     /locus_tag="VBD27_000529"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO7024564.1"
                     /translation="MTYLAPVLTIAGTDPSGGAGIMADLKTFQARRTYGMAVVTSVVA
                     QNTCGVRGVQHIETAIIDQQLACVYDDIKPKAVKTGMLAERETISLVASYLKKYPQPY
                     VLDPVMVATSGHRLIDSDAVEALKEDLLPLATIITPNLPEAEVLVGYDLSDEVSIIKA
                     GYDIQKQYSVRNVLIKGGHLDGLAKDYLFLEKEGLITLSNQRINTIHTHGTGCTFAAV
                     VAAELAKGQSILNAVSTAKSFITSAIETAPELGLGNGPVNHTSYQGD"
     gene            7218..7988
                     /gene="thiM"
                     /locus_tag="VBD27_000530"
     CDS             7218..7988
                     /gene="thiM"
                     /locus_tag="VBD27_000530"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEO7024565.1"
                     /translation="MYLEQLKEVNPLTICITNNVVKNFTANGLLALGASPAMSECIED
                     LEDLLKVADALLINIGTLTKESWQLYQEAIKIANKNQVPVVLDPVAAGASRFRLEVSL
                     DLLKNYPISLLRGNGSEIAALVGEKQASKGADGGKVADLESIAVKANQVFDVPVVVTG
                     ETDAIAVRGEVRLLQNGSPLMPLVTGTGCLLGAVLAAFIGSSDRSDDLACLTEAMTVY
                     NVAGEIAEKVAKGKGVGSFQVAFLDALSQMKSEMIMDK"
     gene            8002..8673
                     /locus_tag="VBD27_000531"
     CDS             8002..8673
                     /locus_tag="VBD27_000531"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEO7024566.1"
                     /translation="MKDTLKLYFVCGTVDCSRKNILTVVEEALQAGITLFQFREKGFT
                     ALQGKEKIAMAKQLQILCKQYQVPFIIDDDIDLVELIDADGLHIGQNDLPVDEARRRL
                     PDKIIGLSVSTMAEYQKSQLSVVDYIGIGPFNPTQSKADAKPAVGNRTTKAVREINQD
                     IPIVAIGGITSDFVHDIIESGADGIAVISAISKANHIVDATRQLRYEVEKALVNRQKH
                     SDVIK"
     gene            8800..10059
                     /locus_tag="VBD27_000532"
     CDS             8800..10059
                     /locus_tag="VBD27_000532"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO7024567.1"
                     /translation="MRKIIINGGKQLTGEVAVSGAKNSVVALIPATILADDVVVLDGV
                     PAISDVDSLVDIMETMGAKIKRYGETLEIDPRGVKDIPMPYGKINSLRASYYFYGSLL
                     GRYGQATLGLPGGCDLGPRPIDLHLKAFEAMGASVSYEGDSMRLATNGKPLQGANIYM
                     DTVSVGATINTMIAAAKANGRTVIENAAREPEIIDVATLLNNMGAHIRGAGTDVITIE
                     GVKSLHGTRHQVIPDRIEAGTYIAMAAAIGRGIKVTNVLYEHLESFIAKLDEMGVRMT
                     VEEDSIFVEEQEKLKAVSIKTSPYPGFATDLQQPLTPLLLTAEGNGSLLDTIYEKRVN
                     HVPELARMGANISTLGGKIVYSGPNQLSGAPVKATDLRAGAALVIAGLMAEGRTEITN
                     IEFILRGYSNIIEKLTSLGADIQLVEE"
     gene            10161..10715
                     /locus_tag="VBD27_000533"
     CDS             10161..10715
                     /locus_tag="VBD27_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7024568.1"
                     /translation="MDIWTNLGRFAFIETEHVNLRPVAYTDREAFWRIASKRTNLQFI
                     FPVQTSKKESDFLLVHSFMKEPLGVWAIEDKVSHKMFGVIRFENIDLSKKTAEIGYFL
                     KESSWGQGIMTECLKTLSFFAFQEFGMDKLIIVTHKENIASQKVALKAHFKQSRSFKG
                     SDRYTRRIRDYIEFQLTRGDYRYE"
     gene            10708..11991
                     /locus_tag="VBD27_000534"
     CDS             10708..11991
                     /locus_tag="VBD27_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7024569.1"
                     /translation="MSKHQEILEYLENLAVGKRVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETRPRSGTVRVAQKAKVNIEKLTYAEIARISDSQVVAGTEGLSKEFSKFSIGA
                     MTHRNIEKYLVQGGLLIVGDRDEIQHLALQHQNAILVTGGFNVSPSVCRLADKLQIPV
                     MVTHYDTFTVSTMINHTLSNAKIRTDLKTVEQVYQSQMDYGFLAQDDTVKEFNLLVKQ
                     TKNVRFPIVNQANVVVGVVSVQDILGKDKEVKLATVMSKNIIVAKPRMSLANISQKMI
                     FEDLNMMPVVSDDFELLGVITRRQAVENLSMSQGTALYTYSDQILSNLQIEDGHFSFL
                     VEPAMIDHTGSLTQGVLTEFLKEICIRVLTRKHQRSIVVKQMTLYFLQPVQIDEIIMV
                     TPTIISEKRREATLDLELKLENKIIAKAMIAVKIN"
     gene            12014..12874
                     /locus_tag="VBD27_000535"
     CDS             12014..12874
                     /locus_tag="VBD27_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEO7024570.1"
                     /translation="MITLKSAREIEAMDRAGDFLASIHIGLRDIIKPGVDMWEVEEYV
                     RRRCKEENVLPLQIGVDGAVMDYPYATCCGLNDEVAHAFPRHYTLKQGDLLKVDMVLS
                     EPLDKSIVDVSSLNFDNVAQMKKYTETYSGGLADSCWAYAVGEVSQEVKDLMSVTREA
                     MYIGIEKAVIGNRIGDIGAAIQDYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            12876..13796
                     /locus_tag="VBD27_000536"
     CDS             12876..13796
                     /locus_tag="VBD27_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO7024571.1"
                     /translation="MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLV
                     TAFPLLVIAANIFPYFHINVSDLLSLMQKNLPKNIYEPASRLAVDAFSKPSTGILGFA
                     SLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLLTFVLIFSTF
                     SKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYFILPNARIRKVRYVIP
                     GTLFSTFVIGFFSNLISQYVLNRVEKMVDIKTFGSVVIFILMLWFIFLAHIMILGAIL
                     NASVQEIATGKIESRRGDIMSLIQKSKEEK"
     gene            complement(13813..14268)
                     /locus_tag="VBD27_000537"
     CDS             complement(13813..14268)
                     /locus_tag="VBD27_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO7024572.1"
                     /translation="MVKLKSLLKKSIQNEVSLYLLFGLLTSLLYLVIRQGIFNFSQDA
                     PFSAIVANIIAILFAFFTNDRFVFKQTKIGQFQRLQTFVIARLGTLGLDLILAVIFVD
                     QFPSIIGQFVQHNLNKINTIESLVSQILIILLNYILSKFVIFKDKKRQL"
     regulatory      14339..14452
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14450..14959
                     /locus_tag="VBD27_000538"
     CDS             14450..14959
                     /locus_tag="VBD27_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO7024573.1"
                     /translation="MNTFTTRDYAHMAIVTAIYIVLTITPPFNAIAYGAYQFRVSEML
                     NFLAFYHRKYLFAVTLGCMISNLYSFGMIDVFVGGGSTLLFVYLGTILFKQYQKDYLF
                     NGLINKAFFFFSFFFAASMITVAVELKIVAGLPLLLTWLTTAVGELASLLVGAVLVDK
                     LSRHVDFTK"
     gene            15099..17057
                     /gene="ligA"
                     /locus_tag="VBD27_000539"
     CDS             15099..17057
                     /gene="ligA"
                     /locus_tag="VBD27_000539"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO7024574.1"
                     /translation="MENRMNELVSLLNQYAKEYYTQDNPTVSDSQYDQLYRELVELEE
                     QHPENILPNSPTHRVGGLVLEGFEKYQHEYPLYSLQDAFSKEELIAFDKRVKAEFPTA
                     SYMAELKIDGLSVSLTYVNGILQVGATRGDGNIGENITENLKRVHDIPLHLDQSLDIT
                     VRGECYLPKESFEAINIEKRANGEQEFANPRNAAAGTLRQLNTGIVAKRKLATFLYQE
                     ASPTQKETQDDVLKELESYGFSVNHHRLISSSMEKIWDFIQTIEKDRISLPYDIDGIV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPNQIKERLAHFASRDAMNITGFGPSLVEKLFD
                     AHLIADVADIYRLSIEDLLTLDGIKEKSATKIYHAIQSSKENSAEKLLFGLGIRHVGS
                     KASRLLLEEFGNLRQLSQASQESIASIDGLGGVIAKSLHTFFEKEEVDKLLEELTSYN
                     VNFNYLGKRVSTDAQLSGLTVVLTGKLEKMTRNEAKEKLQNLGAKVTGSVSKKTDLIV
                     AGSDAGSKLTKAQDLGITIQDEDWLLNL"
     gene            17069..18088
                     /locus_tag="VBD27_000540"
     CDS             17069..18088
                     /locus_tag="VBD27_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO7024575.1"
                     /translation="MKKQKKARLIYNPTSGQEIMKKNVAEVLDILEGFGYETSAFQTT
                     PTKNSARDEAARAAQAGFDLIVAAGGDGTINEVVNGIAPLKRRPKMAIIPTGTTNDFA
                     RALKIPRGNPIEATKLIGKNQIVKMDIGQAQEDNYFINIAAAGSLTELTYSVPSQLKT
                     TFGYLAYLAKGVELLPRVRKVPVKITHDKGEFIGDASMIFVAITNSVGGFEQIAPDAK
                     LDDGKFTLILVKTANLIEIMHLIRLVLAGGKHINDKRVEYIKTSYLTIEPLSDERMMI
                     NLDGEYGGDAPITLANLKNHIRFFANTDEISDDALVLDKDELAIEAIAQKFANEVNDL
                     EGLGD"
     gene            18092..20392
                     /gene="pulA"
                     /locus_tag="VBD27_000541"
     CDS             18092..20392
                     /gene="pulA"
                     /locus_tag="VBD27_000541"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEO7024576.1"
                     /translation="MDNTVIVHYHSRKKNYFNLSLWQWRDGSEGQDAHFSRFDSFGAV
                     AILKYPLPYFLSHYYVIVKDKNWHHKTIDYRIDCTKGGARKEVWIVDGDDNLYYSRQA
                     AVSSHHYSRRQPHAYDMAINSRAFDKKWGFDGWLGFEYSETKTAFRLWAPTAERVELI
                     LYHSTDETASVSKVLSMKRGTAVNYKNHKENTHGVWFTELEGNYNYQAYTYRVYYRRR
                     TFKITRDPYSIATTANGKRSIVIAPEALTPEGFKISHGKEAKWRLENPNQAVIYEMHV
                     RDFSISETSGVKTDYQGKFKGLHQKDTVNQHGDKTTFDYVQDLGVNYIQLQPIFDHHQ
                     TFDDDGHYAYNWGYDPENYNVPEASFSSNPHEPATRILELKSAIQAYHDAGIGVIMDV
                     VYNHTFSSTDSAFQLTVPDYYYRMNHNGTFQNGSGCGNETASEKEMCRKYILDSVLYW
                     VKEYNIDGFRFDLMGLHDVETMNIIRNELNKIDPRILVYGEGWDMGAGLTPQNKAKKD
                     NAYQMPGIGFFNDDVRDAVKGAEIYGEFKKGLVSGNSTEDIVAKGILGSDELVSYIDP
                     SQVLNYVEAHDNYNLNDLLWELHPNDNEKQHIYRVEVASAMNLLMQGMAFMQLGQEFL
                     RTKCYPTGDKGQLTQADKERAMNSYNAPDQVNQVNWDNVTFHKSTINFIRKIITLKTN
                     SPYFSYSSFEEIRKHVFVESAQYHSGFISFTVEEHKKIKIIFNIKGKRLQDPFIGDII
                     ISNHEEGKAIDSLDDELSVMILDITK"
BASE COUNT         6738 a         3054 c         4146 g         6570 t
ORIGIN      
        1 aactaaatta acagttaatc agtatgaagc gattgtgaat agaatgagtg ttgctttaca
       61 aaaattttca aagaatattt ttgagaaaca gaaagtaaat aaagatttaa tccctattgc
      121 gttttggatt gagaaaagtt acagaactgt tggaacgaat gaaatcgccg cttctgtagg
      181 cattcaagga ggattttatc aaaacttcca tgattattat aattattcat atctattaaa
      241 ttctttatgg tatgaaggaa atgtaaaaga agtagttaag gattatgaaa acactattcg
      301 tcaaatacta tctaaaaagc atgagattga aaaaattctt aatcagagca cttctgatat
      361 ctctatagat gatgatgatt acgaaaaagg aaataaagaa ttgctaaggg aaaaattaaa
      421 tattattcta aatctttcaa agagagatta cagagtaact ccatactatg aagtgaataa
      481 actacataca gggcttattt tattggagga tgtccctaat ttaaagattg ctaaggataa
      541 gttgttttca ttagagaatt ctttaaagga atacaaagga gagaaagtta attatgagga
      601 actaagattc aatacggaac ctttaactag ttacttagaa aataaagaaa aatttttagt
      661 ccccaatatt ccatataaaa ataaattaat tttaagggaa gaagataaat atagttttga
      721 agatgatgaa gaagagtttg gaaatgaact tctaagttac aataagctta agaatgaagt
      781 tttacctgtt aatattacaa cttctactat attaaaaccg tttgaacaga agaaaattgt
      841 ggaagatttt aatccttatt ctaatttaga caatttagaa ataaaaaaaa taaggttgaa
      901 tggctcccaa aaacaaaaag tagaacagga aaaaactaaa tcgccaactc ctcaaaaaga
      961 ggctgtaaaa gaacaaactg agcaaaaaat atctgggaag actcaaaacg tagaaaagaa
     1021 atctgaaact gtggcaactc cacaacaaag ttcaactgcg caaacttctg tccaacagcc
     1081 agctccggtt caaccagttg ttcaagaatc taaagcttct caagaggaga ttaatgcagc
     1141 acatgatgcc atttcagcgt ataaatcaac agtcaatatt gctaatacag ccggtgtaac
     1201 aactgcggaa atgacctcgc tcattaatac tcaaacttct aatctttctg atgttgagaa
     1261 agctttaggg aataataagg ttaataatgg tgcagtcaat gtattgagag aagatacatc
     1321 tcgtcttgag aatatgattt ggaatcgtgc ttaccaagct attgaagaat tcaacgtcgc
     1381 tcgtaatact tataataacc aaatgaagtc agaaacagtt ccagttgata atgatattga
     1441 agctatttta gcaggttctc aagctaaaat tagccatttg gacaatcgta tcggagcgcg
     1501 ccacatggat caagcttttg tagctagttt attagaagtt actgagatga gtaaatcaat
     1561 ctcatcgcgt ataaaagaat agacactgct atcaaggcga tcttaaactt ttgtattgag
     1621 ctaacctaaa agatagaaag agactaatat gaaaaaaata acaactttaa tcttagctag
     1681 tagcttatta ctagttgcaa cgacatcggt taaagctgat gataactttg aagtaccaac
     1741 gcgttatgtt aaaatgagtg aaaaatcaaa agcattttat caaagattac aagaaaaaca
     1801 acgtaaaaca catactactg tgaagacttt taataattca gaaataaggc atcaactacc
     1861 tcttaaacaa gaaaaggcta ggaatgatat ctacaattta ggcattctta tttctcagga
     1921 gtctaaaggg ttcatccaac gtattgataa tgcctattct ttggaaaatg tctcagatat
     1981 tgttaatgaa gctcaggctt tgtataaacg caactatgat ttatttgaaa aaatcaaatc
     2041 tacacgtgat aaggttcaag tcttacttgc atcgcatcaa ggtaatacag atttaaaaaa
     2101 cttttatgct gagttggacg atatgtatga acatgtttat ctcaacgaaa gtagagtgga
     2161 ggcgataaac agaaatatcc aaaaatataa ttagtttcta aactaacaaa cattcctaaa
     2221 tataagacat taaaccctac ttattgatta gtgagtaggg ttttactgtt ttaaatagct
     2281 ttctgctcag aatgtaagcc ttgtcatttc aaaggaacta tgttattatt cttaagtaaa
     2341 ttaaatagga catttggggt gcgtaacagc tgagattata cccattgaac ttgatacagt
     2401 tagtactgtc gaagggaaat gtgtgccatt acaagttttt tgtaatggaa aagggcgtag
     2461 tgtcttctta ggagacagct agctctagtt aatcagataa taaactccgt ttgcttgcag
     2521 atggagtttt ttgtcgtcaa aattttttat aggttgttgc cccttatctg tcatagagaa
     2581 ggagttatga tgactaatca taaaacaaaa aaattaacgg ttttagcaat tatgattgct
     2641 ttagatgttt ttctaacgcc catttttaga atagaaggca tggcctttat gtctagtgtg
     2701 gttaatatta tcgctggagt tataatggga ccaatctatg cattagttat ggcacttgta
     2761 acggctttta ttcgtatttt aactcaagca ggagtagcat ctgttgcacc tttagctatt
     2821 ctaggagctg gtccaggagc tttcttagcg ggttggtttt atcggtttta tgcgaaagac
     2881 tggatgtctt gggtgggtga gtttatagga acagggataa ttggagcact tttatcagct
     2941 ccaattatgg tctggttttg gacttggaca gcggatggaa atcacgaagt gttagctaaa
     3001 gcatctgcag ctcaatccat ttttcttttt atgccaaggt ttataggggc gactataatt
     3061 ggcggtgtta ttgctttgtt gctacttcag ggattaaagc gggttcctgt atttatgaca
     3121 atacaagcat tatttaaagg agaaaaagta aaatgaaatt gggtttaaaa gatgtattat
     3181 tgattgcttt attagcggtt gttctgggcg ttgtttactt tggagcaggc tatatttcaa
     3241 atgcttttgt accttttgta ggtcctatag cgcatgaagt tatttatggt atttggtttg
     3301 tggcaggtcc aatggctctt tatatattga ggaaaccagg aaccgctatt gtagcagaat
     3361 tgttagctgc tttgatagag gttttaattg gatcaattta tggaccaagt gtactcgtta
     3421 ttggtaccct tcaaggttta gggtcagaat taggctttac gctgtttagg tatcataatt
     3481 acaagttgcc agcttttata ttgtctgcta ttttgacatc aatttttagt tttgcatgga
     3541 gtttttatgc caatggactt agtgcatttt cattttcata taacattctg atgcttatag
     3601 taagaactgt ttcatctatt attttcttct tattaacaaa gaatatatgt gaccaattgc
     3661 atagaagtgg tgtactgaat gcttatggga ttcatgaagg gaagtagcct atgctatctg
     3721 ttgaaaaatt agcttacacc catggcgata gtcattattt atttgatgaa gtgactttta
     3781 gtcttaaccc aggagaacga attttaatta gtggctattc agggtgtggt aagagtacat
     3841 tggctctttt actatcaggg ttaaaagaaa gtgggaaagg tcaagtttta ttaaacggct
     3901 cattaataga accaagcgat gtgggttttc tttttcaaaa tcctgatttg caattttgta
     3961 tggatactgt ggcgcatgag ttgtatttta ttttagaaaa tttacaaata gaaccggagc
     4021 aaatgcaaga tagaagtgag tttgtattgg cacaagttgg tttaaaagga tttcagaatc
     4081 gtttgattta tactttatct caaggtgaaa aacagcgatt agcattggcg actatttttt
     4141 taaaatctcc taaacttatt attttagatg aagcttttgc gaatttggat caagagagtg
     4201 ccagtcaact attacaatta gttcttaatt atcaagcaaa caatcaatca atgctgattg
     4261 taatcgatca tttgattact tattatcaag atataatgga tcactatttt tggttggaaa
     4321 aatggttgat aagggtcgat tttgattata tgctcaatcg tttgaatgtc ttcgaattag
     4381 aaaagaaaag tcataataca ggtgacaaac tgttatcaat taaagatttc caagttaaac
     4441 tttcaaaaaa caaattcatc tcttaccttg attttgattt agcaagtggg gagaggttgt
     4501 gcttagatgg accaagtggc gtggggaagt ctagtttctt tatgggattg ttagggctct
     4561 ataggactaa aggaaagaaa caatttactc atagaaagca aattcctata agctttttat
     4621 ttcaaaatcc cttagatcaa tttatttttt caacagttta tgacgaaatt tttcaggttt
     4681 gcaaggatag taacaaagcg agggatattt tggaaaccat aaatctttgg gataaaaaac
     4741 agttttcacc atttcaacta agtcaaggcc aacagaggcg attagccatt ggcagtattt
     4801 tagctagtga tagtaaactt ctattattag atgaaccaac ttatggacaa gatgcctatc
     4861 acgctaatat gattacgact cttcttttga gttattgtca caaaaatcat tgtggggtta
     4921 tttttactag ccatgatcct cacttaaaag agtgttttgc aacacgtttc ttggaggtgt
     4981 catcttgttc aatttgaaaa actatcatcc agctatcacc ttcctatgtt tattaatacc
     5041 aaccttttcg tttagtttca cattgaggct aagaacgtct ttgttatttc taattatagt
     5101 agtaacactg caatgcttcg taaaggtttc tttaaaaact tgggcgaagg tcaatttgat
     5161 ctcatttgtg atgggactct ctttattttt agggacgtat ttttggggga aattacccca
     5221 tcaattcgtc ttagctagtc tagtggcctg tcgtccgctt atttttatga acgtgggttt
     5281 gttatttcat gcatcacatt ctaattatga ttttatagaa tctctttatc aaactttcaa
     5341 agtgcctagt cattttgcat atggtatttt tgcggtcttt aatcttttac cattaattaa
     5401 attacaatac caacgtaatc gactcgcatt ccgactgaag aatcaggtaa cttgggcatt
     5461 atcgcctaga ctaatattgt cagttttatt aaagacgata tattgggtag aacagttaga
     5521 gttagcaatg ctttctaaag gttttgaagt gggtaaagag cgaacacatg cttcaactta
     5581 tcccgttcgt tttcgagatt atagtttact tggtatgagt attttattgg ctataggaat
     5641 gatttttaaa taagtacaat tagtttagta atacaattgt gcatttatat agagtgagca
     5701 tgttctgctc atgagatcat aacaaaggag attactatgt taaaagatga gatgattgct
     5761 aaaggaacaa aatatataca atccatctat caagatccat ttattcaagg aattgtaaaa
     5821 gggcgtttag accatgacgt aatctgccat tatttacagg cagataatat ctaccttggg
     5881 aaatttgctg atatttatgc cctgtgcttg gcaaaatctg ataatttacg agataaacaa
     5941 tttttccttg aacaagttga ctttacatta aatcgggagt tagcagatgg tgaaggtcca
     6001 catcaagcct tggcagctta tactaatcga tcttatcagg atattatcga aaaggctgta
     6061 tggtacccgt cagcagatca ttacattaaa catatgtatt ttcattttta cgaaaatggt
     6121 attgctggtg ctttagctgc aatgagccca tgtccatgga tttaccatca attggctaaa
     6181 aaaataattg aggagaacca gtttttgaat ggtaaccctt ttaataattg gataactttt
     6241 tatgctaacg atacagttga agagttaatg gaaaactatt ttcgtatgat ggattattat
     6301 gcgcagaatt tgtcaaaaga gaagcaagct gatttggtag atgcttttgt gaaatcttgt
     6361 cagcatgaga gacgtttttt tcaaatggca ataaatcaag aaaaatggga ggattaatat
     6421 gacttattta gcaccagttt taactattgc aggaactgat cctagcggtg gagcagggat
     6481 tatggcagat ttaaagacat ttcaagctcg tcggacttac gggatggctg ttgtgactag
     6541 tgttgttgct cagaacactt gtggggttag aggcgttcag catatagaaa cagctatcat
     6601 tgatcaacaa ctagcttgtg tttatgatga tattaagcca aaggcggtca aaacaggaat
     6661 gttagcggag agggaaacga ttagtttagt cgcttcatat ttaaaaaaat atccgcaacc
     6721 ttatgtatta gatcctgtta tggtggcaac tagcggacat cgtttaattg attcagatgc
     6781 cgtagaagca ttaaaagaag acttattgcc attagcaact attatcacac caaatttacc
     6841 agaagctgag gttttagtgg gatatgactt atcagatgaa gtaagtatta ttaaagcagg
     6901 ttacgatatt caaaaacaat attctgttag aaatgttctt ataaagggag gacatctaga
     6961 tggtttggct aaagattatt tatttttgga aaaagaaggg ttgataactc ttagtaatca
     7021 acgtatcaat actattcata ctcatgggac tgggtgtaca tttgcagcag tcgttgcagc
     7081 ggagttagct aaagggcaat caattctaaa cgcggtaagt accgctaaat catttattac
     7141 ttcagctatc gaaacagcgc ctgagttggg tcttggaaac ggtcctgtca atcatactag
     7201 ctatcaagga gactaaaatg tatttagaac aactaaaaga ggtaaatcct ttaaccattt
     7261 gtatcactaa taatgtggtc aaaaacttta cagccaatgg tttattagct ttaggagctt
     7321 ctccggctat gagcgagtgc atagaggact tagaagactt gttaaaagtt gcagatgcat
     7381 tattaattaa tataggaaca ctaacaaaag aaagttggca actttaccaa gaagcaatca
     7441 aaatagcaaa taaaaaccaa gttccagttg tattagatcc tgtagcggca ggagcaagtc
     7501 gttttcgatt agaagtgtca cttgatttat tgaagaatta tcctattagt ttacttagag
     7561 gaaatggtag tgaaatcgct gctttggttg gtgaaaaaca agcgtctaaa ggggcagatg
     7621 ggggaaaagt ggctgattta gaatcaattg ctgtaaaagc caatcaagtt tttgatgtgc
     7681 cagtggttgt cacaggagaa actgatgcta tcgctgttag aggagaagtt cgcttattac
     7741 aaaatggtag tcctttaatg ccacttgtta caggaactgg atgtctatta ggggcagtat
     7801 tggctgcttt tattggtagt tctgacagaa gcgatgatct tgcttgtctg acagaggcca
     7861 tgactgttta taatgttgct ggagaaattg cagagaaagt ggcaaaaggt aagggagtgg
     7921 gaagtttcca agttgcattt ttagatgccc tttctcagat gaaatcagaa atgattatgg
     7981 acaaataggc ggtgtcagta gatgaaagat acattgaaat tatattttgt ttgtgggact
     8041 gtggattgct ctaggaagaa tatattgact gttgttgaag aagccttaca agcaggtata
     8101 actctatttc aatttagaga aaaaggtttt acagcattac aaggtaagga aaaaattgct
     8161 atggctaagc aactccaaat actttgtaag caatatcaag ttccttttat tattgatgat
     8221 gacattgact tagttgagtt aattgatgct gatggtcttc atattggaca aaatgattta
     8281 cctgttgatg aggcacggag acgtttacca gataaaatca tcggtttatc tgtgagtaca
     8341 atggctgaat atcagaaatc acaattatct gttgttgatt acatcggaat tggtcctttt
     8401 aatccgacac aatcaaaagc agatgctaag cctgctgttg gcaataggac aacaaaggca
     8461 gttagagaaa ttaatcaaga tattccaatc gttgcaatag gcggaatcac tagtgatttt
     8521 gttcatgata ttatagaatc aggcgcagat gggattgctg ttatatcggc tatttcaaaa
     8581 gcaaatcata ttgtagatgc aaccagacaa ttgcgctatg aagttgaaaa agctctggtt
     8641 aataggcaaa aacacagtga tgtaatcaaa taaggctaca tcgttaatta taagaatttt
     8701 taagatatta tatcatcaca caatactggt atttgaaaaa gaatttaaac tatgataata
     8761 tagtagagtt gaattctagt taaagaaaga tttgaaagta tgcgtaaaat tattattaac
     8821 ggtggcaagc aactgacggg agaagttgct gtatcgggtg ccaaaaatag tgtagttgcc
     8881 ttgattcctg ccactattct tgctgatgat gttgtggtct tagatggagt tccggctatt
     8941 tctgatgttg acagtttagt tgacattatg gaaactatgg gagctaagat taagcgttat
     9001 ggtgagacgc ttgaaattga tccacgtggc gttaaagata ttccaatgcc ttatggcaaa
     9061 atcaatagtc ttcgtgcatc ctattatttc tatggaagct tattggggcg ttatggtcaa
     9121 gcaacattag gtttacctgg tggttgtgat ttaggccccc gtccgattga tttacattta
     9181 aaagcttttg aagcgatggg agctagtgtt tcttatgaag gtgattcaat gcgtctagca
     9241 acaaacggaa agcctttgca aggagcaaat atttatatgg atactgtcag tgttggtgcg
     9301 acaattaaca ctatgatcgc agcagctaag gcaaatggtc gtacggttat tgaaaatgct
     9361 gcgcgtgaac cagaaattat tgacgtggct actcttttaa ataatatggg agctcatatt
     9421 cgtggtgctg ggactgatgt tattacaatt gaaggtgtga aatcgctcca tggcacgcgt
     9481 catcaagtaa ttccagatcg tattgaagca ggaacttata ttgctatggc agcagctatc
     9541 ggacgaggaa taaaagtgac caatgtgtta tatgaacacc ttgagagttt cattgctaag
     9601 ttagatgaga tgggagttcg aatgacagtt gaagaggatt caatttttgt cgaagaacaa
     9661 gaaaagttaa aagctgtatc cataaaaacc tcaccttacc caggatttgc tacagattta
     9721 caacaacctc taaccccttt attactaaca gcagaaggaa acgggagttt attagatact
     9781 atctatgaga aacgtgttaa tcatgttccc gaattagcac gtatgggagc taatatttct
     9841 accctagggg gcaaaatagt ttatagtggt cctaatcaat tatctggtgc acctgtaaaa
     9901 gcgacagatt tgcgtgcagg cgctgcttta gttatcgctg gcttaatggc agagggacga
     9961 actgaaatta cgaatatcga gtttatttta cgtggatatt ctaatatcat cgagaaacta
    10021 acatcattgg gtgcggatat ccaattggtt gaggaatagt atatataaca aagagttgag
    10081 aatatttctt agctcttttc ttcttgtttt agattaagtc acttataggt atttgaaatc
    10141 tcttgtggta taatattatc atggatatat ggacaaattt aggacggttt gcattcattg
    10201 aaacagaaca tgttaaccta cgtcctgttg cctacactga tagggaagct ttttggcgaa
    10261 tagcttcaaa aaggacaaat ttacaattta tttttcctgt tcaaaccagt aagaaggaaa
    10321 gtgattttct tttagttcat tcttttatga aagagccgtt gggagtttgg gcgattgaag
    10381 ataaagttag tcataaaatg tttggagtta tccgttttga aaacattgat ttatctaaaa
    10441 agacagctga aattggctat tttttgaaag aaagttcttg gggtcaaggc attatgactg
    10501 aatgtctaaa gacactttct ttttttgctt ttcaagagtt tgggatggac aaattgatca
    10561 tagtaacaca taaagagaat attgctagtc agaaggttgc tttgaaggcg catttcaaac
    10621 aatctaggag ttttaaagga agcgaccgtt atacacgccg tataagagac tatattgagt
    10681 ttcaattaac taggggtgat tatcgttatg agtaaacatc aagaaatttt ggagtaccta
    10741 gaaaatttag ctgttggtaa gagggttagt gtacgcagta tttcaaatca tttaaaggtg
    10801 agtgatggca ctgcataccg cgctataaaa gaggcagaaa ataggggtat tgttgagact
    10861 agaccgcgta gtgggacagt acgtgttgct caaaaagcaa aggttaatat tgaaaaactg
    10921 acttatgctg aaattgcaag gattagtgac tcacaagttg ttgcaggaac agaggggctc
    10981 agcaaggagt ttagtaaatt ttctataggt gcaatgactc acagaaatat tgaaaaatat
    11041 ttggtgcaag gagggttatt gattgttggc gaccgtgatg aaatacaaca tttagcctta
    11101 caacatcaaa acgctatctt agttacaggt ggctttaatg tttccccatc tgtttgtcgt
    11161 ttagcagata aacttcaaat tcccgtaatg gtaacacact atgatacatt cacagtatca
    11221 acgatgatta atcacacttt gtcaaatgcc aaaattcgta ctgatttaaa gaccgttgaa
    11281 caagtctatc agtcgcaaat ggattacggt ttcttagcgc aggatgatac cgtaaaagaa
    11341 tttaatcttc ttgttaaaca aactaaaaat gttcgttttc caatagtcaa ccaagctaat
    11401 gttgtcgttg gtgttgttag tgtgcaagat attcttggaa aagataaaga agtaaaattg
    11461 gcaacagtca tgtcaaaaaa tatcattgtt gctaaacctc gaatgagtct agctaatatc
    11521 agtcaaaaaa tgatttttga agatttgaat atgatgccag tagtatcgga tgactttgaa
    11581 ttattaggcg ttattacaag acgacaagct gtagaaaatt tatcgatgtc gcaagggaca
    11641 gctttataca cctatagtga tcaaatcttg tctaatttac aaattgaaga tggtcacttt
    11701 tcgtttttag tagaacccgc tatgattgac catacaggta gcttaacaca aggagttttg
    11761 acggagttct taaaagaaat ttgtattcgt gtcttaactc gtaagcatca acgcagtatt
    11821 gttgttaaac aaatgacact ttatttcttg caacctgttc aaattgatga aataataatg
    11881 gtaacgccaa caattatttc agaaaaacgg cgcgaagcta cactcgattt agaattaaaa
    11941 ttagaaaata aaattattgc taaagctatg atagcggtaa aaattaatta gatataagga
    12001 gaataaatag aaaatgatta cattaaagtc agcacgcgaa attgaagcaa tggatagagc
    12061 aggtgatttt ttagccagca tccacattgg acttcgtgat attatcaaac caggagttga
    12121 tatgtgggaa gttgaagaat acgttcgtag acgttgtaaa gaagaaaacg tattgccatt
    12181 gcaaatcggg gttgatggtg cagttatgga ttacccatat gctacttgtt gtgggcttaa
    12241 tgatgaagtg gctcatgcct ttcctcgtca ttatacccta aaacaaggtg accttttaaa
    12301 agtagatatg gttttaagcg aacctttaga taaatcaatt gttgatgttt catcgttaaa
    12361 ctttgacaac gtggctcaaa tgaaaaagta cacggaaaca tatagtggtg gtttggcaga
    12421 ctcatgttgg gcttatgctg taggtgaagt ttcacaagag gtgaaagact taatgtctgt
    12481 gacaagagaa gcaatgtata ttgggattga aaaagcagtt atcggaaacc gtattggtga
    12541 tattggagct gctattcagg attatgcaga gagtcgtggt tatggagttg ttcgtgattt
    12601 ggttggtcac ggtgttggtc caacaatgca tgaagagcct atggtgccta actatggtac
    12661 agcaggtcga ggtctccgtc ttcgtgaggg gatggtttta accatcgaac caatgattaa
    12721 tactggtact tgggaaattg atacagacat gaaaactggt tgggcacata aaaccttaga
    12781 tggtggtctt tcttgtcaat atgaacatca atttgttatc actaaagatg gtcctgttat
    12841 tttaacgagt caaggagaag aaagaactta ctaaaatgaa attaaaaaaa ttctttgagg
    12901 atttgctggc taaactagaa tatagaccca ttcaagtttt tatgcgtcat tttcaaagtg
    12961 cagagatgga cttatcagcc atagcagttg cttattatct attagtgaca gcttttccat
    13021 tactagttat tgctgctaat atttttcctt attttcatat taatgtgtcg gatttattgt
    13081 cacttatgca aaaaaatctt ccgaaaaata tttatgaacc tgcctcacgc ttagcagtag
    13141 atgctttttc gaaaccgtct accggcattt tgggattcgc gtcattaaca gctttttgga
    13201 caatgtctaa aagtttgacc tctcttcaaa aagccattaa taaagcatac ggtgttgacc
    13261 aacacagaga ctttgtgatt agtcgtttag tgggtgtggg gactgggctg atcatcctat
    13321 ttttactgac ttttgtttta attttttcaa ccttttcaaa accagtttta cagataattg
    13381 taaacatgta tgatttggga gatactctaa cggcgtggct tttaaatctt gcacaaccag
    13441 ttacattttt gactatattt ttaggtattg gaattttata ctttatttta cctaacgcac
    13501 gaattagaaa agtacgttat gtcattccag ggacattatt ttcaacgttt gttattggtt
    13561 tttttagtaa tttaattagc caatacgtct taaatagggt tgaaaaaatg gtagatatta
    13621 aaacgtttgg ttcggtcgtt atctttatct taatgctatg gtttattttc ttagcccaca
    13681 ttatgatttt aggtgcaata ctgaatgcca gcgttcaaga aattgcgaca ggaaaaattg
    13741 aatcaaggcg tggagatatt atgtctctta tccaaaaatc caaagaagaa aaataagaaa
    13801 agacagctct tgttagagtt gccttttttt gtctttaaaa ataacaaatt tgctcaagat
    13861 gtagttgagt aaaataatta atatttgaga tacaaggctt tcaatggtgt tgattttatt
    13921 taaattgtgc tgaacgaatt gtccgataat agaaggaaat tgatcaacaa aaataacagc
    13981 taatatcaaa tctaatccta atgtgcctaa tctagcgatg acaaatgttt gtaagcgttg
    14041 aaactgtcca attttagttt gtttaaaaac aaatcgatca tttgtaaaaa aagcaaaaag
    14101 tatagctatt atgttagcaa ctattgcaga gaatggagcg tcctgcgaga aattaaaaat
    14161 cccttgtcga ataactaaat aaagaaggga tgttaatagt ccgaacagta aatataagct
    14221 gacttcattt tgaatacttt ttttcagtaa cgattttaat ttaaccatat ttttattata
    14281 tcacggatgc ttcaaagtga tgaggaaatc tcccataagc ttggctttac tttttgtttg
    14341 tgatatactt agtaagttgt tttacacaac ccttgatgct tagttccctt tcatcaagca
    14401 tattacaaac gttagaagtg ttaactgtct aaacgttaaa ggagaaatta tgaacacatt
    14461 tacaactcgc gattacgctc acatggctat tgtaacagct atctatatcg ttttaaccat
    14521 aacgccacct tttaatgcta ttgcttatgg tgcttatcaa ttccgtgttt cagaaatgct
    14581 aaatttcttg gccttttatc accgaaaata cctttttgcg gtaacgttag gatgtatgat
    14641 ttctaatctt tatagttttg gaatgattga tgtctttgta ggtggtggtt caactttact
    14701 ttttgtttat cttggaacca ttttattcaa gcaatatcaa aaagattatc tatttaatgg
    14761 ccttattaac aaagcattct ttttcttttc tttcttcttt gctgcttcaa tgattacggt
    14821 agcagttgaa ttgaaaattg tagcaggatt acctttatta ttaacatggt taacgacagc
    14881 tgttggtgaa ttggcttcac ttttggttgg agcagtcctt gtggataagt tgagtagaca
    14941 cgtcgatttt acaaaataaa tatattataa agcgcgagac attgatctcg gcttttttgt
    15001 ttggttttat agttagcata gctagaaaat gctgcaactt ttatgagaaa tgctccaatt
    15061 tttattaata tccttagtct ttgtgataaa atagaaatat ggaaaatcgt atgaatgaat
    15121 tagtatcttt gttgaatcag tacgcaaaag agtattatac acaagataat cccacagttt
    15181 ctgacagtca atatgaccag ttatatcgag agttagtaga attagaagag caacatccag
    15241 aaaacatttt gccaaatagt cccacacatc gagtgggtgg tttagtttta gaaggctttg
    15301 aaaaatacca acatgaatat cctttgtatt cattgcagga tgctttctca aaagaagaat
    15361 tgatagcctt tgataaacgt gttaaagcag agtttccaac tgcttcttat atggcagagc
    15421 tgaaaattga tggtctatct gtctcgttga cttatgtaaa tggtatttta caggttgggg
    15481 caacacgtgg tgatggcaat attggagaaa atattactga aaatttaaag cgtgttcatg
    15541 atattcctct acatttagac caaagtttag atattactgt tcgcggagag tgctaccttc
    15601 ccaaagaatc ttttgaagct attaacatag aaaaacgtgc aaatggtgag caggaatttg
    15661 ctaatcctag gaatgcagca gcaggaacct tgcgccaatt aaacacaggg attgttgcga
    15721 aacgtaaatt agcaactttt ctttatcaag aagctagccc tacgcaaaaa gaaacacaag
    15781 atgatgtttt aaaagagcta gaaagctatg gcttttcagt caatcatcat cgtttaatta
    15841 gctcttctat ggagaaaatt tgggatttta tccaaactat agaaaaagat agaatttccc
    15901 tcccttatga tatcgatggt attgttatta aggttaatag cttagcaatg caagaagagt
    15961 tgggcttcac tgtaaaggca cctcgttggg cgattgcata taaattccct gctgaagaaa
    16021 aggaagcaga aatattatct gttgattgga cagttgggcg aacaggagtt gtaacaccaa
    16081 cagctaacct aactccagtt caactagcag ggacaaccgt tagtagggcc actctgcata
    16141 atgtagatta tattgccgaa aaagatatcc gtattggaga tactgttgtt gtatacaaag
    16201 caggggatat catccctgca gtcttaaatg tcgttatgag taagcgaaac cagcaagaag
    16261 tcatgcttat tcctaaacta tgtccttctt gtggtagtga attagttcat tttgaagatg
    16321 aggtggcttt acgttgtatt aatccgctgt gccccaacca aatcaaagaa cgtttagctc
    16381 attttgctag ccgggatgct atgaatatta caggttttgg tccatcctta gtcgaaaaat
    16441 tatttgatgc tcatttaata gcagatgttg cagatattta tcgtttaagt atagaggatt
    16501 tattaacttt agatggtatt aaagagaaat ctgcaactaa gatttatcat gcaatccaat
    16561 cttcaaaaga aaattctgct gaaaaacttc tttttggcct aggtattcgt catgtgggaa
    16621 gcaaggcgag ccgcctttta ttggaggaat ttggtaattt gaggcaacta agtcaagcaa
    16681 gtcaagaaag tattgcctca attgatggac taggaggggt tattgccaaa tcacttcata
    16741 cgttctttga aaaagaagag gtagataaac tactagagga attaacttcc tataacgtta
    16801 attttaatta tctaggaaaa cgagttagta cagatgcaca actttcaggt ttaacagtcg
    16861 ttttaaccgg aaaacttgaa aagatgactc gtaatgaagc aaaagagaaa ttacaaaacc
    16921 ttggtgctaa ggtgacaggt agcgtttcta aaaagactga tttaattgtt gcaggaagcg
    16981 atgcaggaag taaactaacc aaggctcaag atttagggat tacaatacaa gatgaagact
    17041 ggttactcaa cctttaagtg aggaagaaat gaagaaacaa aaaaaagcgc gtttaattta
    17101 taacccaact tctggtcaag aaattatgaa aaaaaatgta gcggaagttc ttgatatact
    17161 tgaaggattt gggtatgaaa catcagcctt tcaaacaacg ccaactaaaa attctgccag
    17221 agatgaagct gctagagctg ctcaagctgg ttttgatttg attgttgcag caggtggaga
    17281 tggtactatt aatgaagttg ttaatggtat tgctcctttg aaacgtcgtc ctaagatggc
    17341 aattattcca actggaacaa ctaatgattt tgctcgtgcc ttaaaaatac cgcgaggtaa
    17401 tcctattgaa gcaactaaat taattggtaa aaatcaaatc gttaagatgg acattggtca
    17461 agctcaagag gacaattatt ttattaatat tgcagctgca ggtagtttga cagagttaac
    17521 ttatagtgta ccaagtcaat tgaaaacaac gtttggctat cttgcttatc tagcaaaagg
    17581 tgttgaatta ttacctcgag ttcgaaaagt accagtcaaa attactcacg ataagggtga
    17641 atttatcggt gatgcttcaa tgatttttgt tgccatcaca aattctgtag gagggtttga
    17701 acaaattgca cctgatgcca aattggatga tgggaaattt acccttattt tagtaaaaac
    17761 tgcaaatctt attgagatta tgcatctcat tcgtttggtg ctagcaggtg gaaaacatat
    17821 taatgataaa cgagtagagt atattaaaac gtcttatcta actatcgaac ccctatcaga
    17881 cgagcgtatg atgattaact tagatggaga atatggagga gatgctccca ttactctagc
    17941 aaaccttaaa aatcatatta ggttctttgc taatactgat gaaatttcgg atgatgcttt
    18001 agttttagat aaagatgaat tagcgataga agctattgca cagaagtttg ctaatgaggt
    18061 aaatgattta gaaggattag gagattgatg aatggataat actgtcattg tacattacca
    18121 tagccgaaag aaaaattact ttaatttaag tttatggcag tggcgcgatg gctcagaggg
    18181 tcaagatgct catttttcac ggtttgacag tttcggtgct gttgcgatac taaaatatcc
    18241 cttgccatat tttttaagcc attattatgt gattgttaag gataaaaatt ggcatcataa
    18301 aacaatagat tatcgcattg attgtactaa aggtggtgca cgtaaagaag tttggattgt
    18361 agatggggat gacaatctgt attattctcg tcaagcagca gttagtagcc atcattatag
    18421 tagacgccaa cctcatgcct acgatatggc aatcaatagt agagcgtttg ataaaaaatg
    18481 gggatttgat ggttggttag gttttgaata ttcagaaact aaaacggctt ttagattgtg
    18541 ggcccctact gcggaacgtg tagaactgat tttatatcat tcgacagatg aaacagcaag
    18601 tgtatcaaaa gttctttcta tgaaaagagg gacagccgtc aattataaaa atcataaaga
    18661 aaacacccat ggcgtctggt ttactgaact tgaaggaaac tataattacc aagcatatac
    18721 ttatcgagtc tactatagac gacgtacctt taaaattacg agagaccctt attccattgc
    18781 aacgactgca aatggtaaaa ggtctattgt tattgctcca gaagcattaa ctccggaagg
    18841 gtttaaaata agtcatggaa aagaagctaa gtggcggctc gaaaacccca atcaagcagt
    18901 catttacgaa atgcatgtgc gtgatttttc gatatctgag acttcaggag ttaagacaga
    18961 ttatcaagga aaatttaagg gattgcatca aaaagataca gttaatcagc atggtgataa
    19021 aacgaccttt gattatgtgc aagacttagg agttaactat atccaacttc aaccaatttt
    19081 cgatcatcat cagacgtttg atgatgatgg tcactatgct tataattggg gatatgatcc
    19141 tgaaaactac aacgttccag aagcaagctt ctctagtaat ccacatgaac ctgcaacgcg
    19201 tattttagaa ttaaaatctg ctatccaagc gtaccatgat gcaggcattg gtgttattat
    19261 ggatgttgtc tataatcata ctttttcatc aactgattct gcatttcaac taacggttcc
    19321 agattattat tatcgaatga atcacaatgg aacatttcaa aatggttcag gctgtggtaa
    19381 tgaaacagct agtgaaaaag aaatgtgtcg taagtatatt cttgattcag ttttgtattg
    19441 ggtgaaagaa tataatatcg atggttttcg atttgatttg atgggactac atgatgtaga
    19501 aacgatgaac attataagaa atgagctaaa taaaatcgac cctcgtattt tggtttatgg
    19561 agaaggatgg gacatgggag ctggtctcac gcctcaaaat aaagctaaaa aagataatgc
    19621 ttatcagatg cctggaattg gctttttcaa tgatgatgta agagacgctg tcaaaggtgc
    19681 ggaaatttac ggagaattta aaaaaggctt agtttctgga aacagtacag aagatattgt
    19741 tgcaaaaggc attttaggaa gtgatgagtt agtatcatac attgatccta gtcaagtttt
    19801 aaattatgtt gaagcacatg acaactacaa cttaaatgat ttgctatggg aattacatcc
    19861 taatgacaac gagaaacaac atatttaccg agttgaagta gctagtgcta tgaatctatt
    19921 gatgcaaggg atggctttca tgcaattagg tcaggagttt cttcgaacaa agtgttatcc
    19981 tacaggtgat aaaggtcaac ttactcaagc agataaagaa agagctatga attcttacaa
    20041 tgccccagac caagttaatc aggtcaactg ggataatgtt acctttcata agagtactat
    20101 taattttatt aggaaaataa ttactttgaa gacaaactct ccttactttt cttactctag
    20161 ctttgaagag attcgcaaac atgtttttgt agagtctgct caataccatt caggtttcat
    20221 aagttttaca gtcgaagaac ataagaaaat aaaaataatt ttcaatatta aaggaaaacg
    20281 cttgcaggat cctttcatag gtgatataat aatatcaaat catgaagaag gaaaagcgat
    20341 agattcatta gatgatgagc tttctgtaat gatccttgat atcacaaaat aacctgaaaa
    20401 cattcaggtt atttttgtgt atattaagga atttgatttg aatttgagtt tcttatattt
    20461 tagttaattt ttcggaaata tagagggtga aatattttca gatgcgtc
//
LOCUS       DATRQA010000020        20387 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.020, whole genome shotgun sequence.
ACCESSION   DATRQA010000020 DATRQA010000000
VERSION     DATRQA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20387
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.020"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            381..719
                     /locus_tag="VBD27_000542"
     CDS             381..719
                     /locus_tag="VBD27_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO7024577.1"
                     /translation="MRPQKLTIAGGRRTTVDYDDRSAEYRDYNRNRWKYDKQVKQFYN
                     SKLWRETSKQVLLQNDYVCAMCGGEATMTDHIVSVKKDWNRRLDWNNLQASCKACNDS
                     KAIRERCKNN"
     gene            890..1357
                     /locus_tag="VBD27_000543"
     CDS             890..1357
                     /locus_tag="VBD27_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEO7024578.1"
                     /translation="MGRKLKVVETTKKHLTKEEKIARETAQNKASDGLDKLQITPPRH
                     LNEVARAEYRRIINDLQTLPVRNLDRGLLELYCSWYAIYKETTKKLDEIGYFTNDPDK
                     GLIPSPLILTLEKATANIRSSASQLGLTVDSRMKMFIPKEEEKQKSIFDKFGG"
     gene            1372..3126
                     /locus_tag="VBD27_000544"
     CDS             1372..3126
                     /locus_tag="VBD27_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEO7024579.1"
                     /translation="MTIEYDYSAISDIYKDDTFYYAKKIVDEEIKASKKVFKACLRHL
                     NDLKRIEDEDFKFIYLPQKASDPINFIEILPDVKTGKPYPLAMFQKFIIGNLYGWRKK
                     TDHSLRRFRKAMISVARKNGKTILIAGILLYEFLFGHNPSMSRQLFCTANDRTQAKIA
                     WDMAKKQLASLRAKDADVRKATKIVRDELKNLHDESYIRALSRDTGAVDGFEPYVGVL
                     DEFAASKTNEMLELLESGQGQLDNPFILIISTAGMDLNVPMHTIEYPYITKILDGEIT
                     DEGYFGYVAEQDNEEEIKDETNWIKSNPILEVDTLHDKLMDYLRTRRKVSLETGEVNK
                     VLIKNFNMWRQSSEESYIDKQSWELAKIDKPDTYKRRVWLGVDVGRVSDLFAISPVVM
                     MDDYWYVDSFSFVATKYGLTAKEKRDGVSYSNLERQGYCEITTLESGVIDDERVLEKI
                     EELIYINEWEVHGICFDPYQFGTLLTMIEKRHPEWPLIEVSQTTMVLNMPTKQFRDDL
                     KKGKIKHSGNPLLTMAANNAYIKTDNNGMRIDKNKNSNKIDPLDAVLDGYAVCYLEPF
                     DGSGYWTSEKILGGETLF"
     gene            3123..3290
                     /locus_tag="VBD27_000545"
     CDS             3123..3290
                     /locus_tag="VBD27_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024580.1"
                     /translation="MIGFILKNIHTLILLVGLGLLIYGLFLFGDKVGFIASGIILVIL
                     AIYVDSVGGRK"
     gene            3287..3517
                     /locus_tag="VBD27_000546"
     CDS             3287..3517
                     /locus_tag="VBD27_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024581.1"
                     /translation="MNKRIKKKRKLEHYIASLIAENVMFSKELIKQHERIEQLEKIVE
                     HNAQATNNELSRIKKHSKKKWKNNVTLPIERR"
     gene            3524..4744
                     /locus_tag="VBD27_000547"
     CDS             3524..4744
                     /locus_tag="VBD27_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO7024582.1"
                     /translation="MSFFQPLGTSKVSYDDYIASVLAGDVSQQYLGVSALKNSDVLTA
                     TSIIAGDIARFPLVKKDINGDIIHDEDINYLLNVKSTNNASARTWKFAMAVNAILTGN
                     SFSRILRDPKTGQALQFQFYRPSETTVEETENHEIIYTFTDTLTAKEIKCFAHDVIHW
                     KFFSHDTILGRSPLLSLGDEIDLQTGGINTLIKFFKDGFSGGILTMKGAQLSGEARQR
                     ARQDFEKMREGSVGGSPLVFDSTMEYTPLEIDTNVLQLITSNNFSTAQIAKALRVPSY
                     KLGVNSPNQSVAQLMEDYVTNDLPFYFDAITSEIGLKTLNDGDRRLYHVEFDTRSVTG
                     RNVDEIVKLVNNQILTPNQGLVELGKQKSKDPNMDRYQSSLNYVFLDKKEEYQDQVGV
                     KGKRGEVNAKENKP"
     gene            4722..5387
                     /locus_tag="VBD27_000548"
     CDS             4722..5387
                     /locus_tag="VBD27_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEO7024583.1"
                     /translation="MPKKINLKGPLIANNSQEVYDYYGMEAVSAKSIIDKLPEDNSDI
                     ILEVNSNGGLVTVGSEIYTALRNYKGKVTAEITGMAASAASVAVMGADKVVMSPTAQM
                     MVHKALFNWVSGNSDDLDKASSALKSSDKAIVNAYVAKTGKSEDEIMQLMRNETFMSA
                     QDAVESGFADEVMSFEAIASIDNQMLPQAVIDDYYASRSKRKQEINNMLLEIEKEEIL
                     QGL"
     gene            5411..6595
                     /locus_tag="VBD27_000549"
     CDS             5411..6595
                     /locus_tag="VBD27_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEO7024584.1"
                     /translation="MFDEKIEEIKADITELNKVIAAKTAEVKAALESDDLETARSVKA
                     EVEATKAKLVESENDLKLYESSVEVGGAENIGGKEVTQEEKTYRESVNDFIRSKGKIV
                     NDSLRFEGKDEVLMPINETTPVEPKKDGVKKTDVKPVSSEEILYTPAREVKTVVDLKQ
                     FTSIHQAKKASGKWPVLQRATEKMVSVEELEKNPKLGKPQFTNVAWEIKTYRGAIPLS
                     QESIDDADVDLVGIVAETIGQMKVNTTNDAIATVLKEFEAKTVKNLDEIKKLLNVELD
                     PAYNVSFIVSQSFYQTMDTLKDKNGRYLLQDSITSVSGKVFLGKPVFVLADEVLGKDT
                     AFVGDFKRGVLFADRKDLGLRWADNEIYGQYLQAVLRFGVSKVDDKAGYYVTFKPEQL
                     PS"
     gene            6609..6770
                     /locus_tag="VBD27_000550"
     CDS             6609..6770
                     /locus_tag="VBD27_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024585.1"
                     /translation="MAQPEVSSATVVGKPTKDSTIEDIKKYLTSQNIDFSGKTLKADL
                     LKLAGVEEV"
     gene            6773..7075
                     /locus_tag="VBD27_000551"
     CDS             6773..7075
                     /locus_tag="VBD27_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO7024586.1"
                     /translation="MTVSKELLDSVKLYCKIDFDFEDDIIEEMIASAQEQICFAIEEG
                     STPEMFKNYAKFSLAVKKQVKEEYDHRGLSADTNRYPLANGVLNIIHQLRLRGDGA"
     gene            7072..7419
                     /locus_tag="VBD27_000552"
     CDS             7072..7419
                     /locus_tag="VBD27_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEO7024587.1"
                     /translation="MITRKMNVRIAIFSQTGGQNEDGEVVSAIRKDLYTCWAEVSKTQ
                     LRDFNYQSRYQNASNLPTNKDTKVFLIRYNPKLSIDNTMFIEHNKHIYKIDKIEFDES
                     GKEMIILSGVSIS"
     gene            7416..7793
                     /locus_tag="VBD27_000553"
     CDS             7416..7793
                     /locus_tag="VBD27_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO7024588.1"
                     /translation="MTKGLDEILANITKLEVKSPKIAKEAVTQVAEEFEKALTKNTPV
                     DYSVHDTKLKYDTAISGFKGANVGITSKKIGYGRKTGWRAHYPNNGTIYQKGQDFKEK
                     TINEMTPRAKEIYAKKIKEGLGL"
     gene            7790..8215
                     /locus_tag="VBD27_000554"
     CDS             7790..8215
                     /locus_tag="VBD27_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024589.1"
                     /translation="MIAETTAYKLLKNDRTLNELLDKLRGGPFKNGFDQGIFTYDIPD
                     NPVDLRKVELAPFMRINTTYDGPSLYADDDMISNEQRITINFWCKTASQSDQIASCID
                     RILKNGGFERYTANEKPRYKDSDIDLLMNVRKYRYFDFY"
     gene            8231..8827
                     /locus_tag="VBD27_000555"
     CDS             8231..8827
                     /locus_tag="VBD27_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7024590.1"
                     /translation="MGKVKFGLRDFQYAVLGDNDKVNEKDKGIKALPGMKSAKLDITN
                     ELVTVMADDGPYVVLSGGITETKLEIEVLDLTSEARQDFFGIKADKGIERYNKSLTPN
                     NVACMFRTSDENGKAIWVGLLKGKFNIPGMDTKTKEGAPNPEPDKVTGNFVARGTDGD
                     VVVIGREGVNGFELKKFKELVFDGVTGLEKSSEDNSLM"
     gene            8880..9200
                     /locus_tag="VBD27_000556"
     CDS             8880..9200
                     /locus_tag="VBD27_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024591.1"
                     /translation="MYEITLKKGGVDKTFSKDFVNVEDNMLAVEHQVRQSAIFSDDKR
                     RLDDKEHRKLNESYLQMFVEMYGNQFNVDDLKQSNVSVLDKLTELFVDALGGEKEEDE
                     KKEQ"
     gene            9230..9397
                     /locus_tag="VBD27_000557"
     CDS             9230..9397
                     /locus_tag="VBD27_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024592.1"
                     /translation="MWIQSLLKNGYTILDIKKMRLSDIELMVKALETDFAEKEEVVET
                     TLDKAFPFLFG"
     gene            9410..13351
                     /locus_tag="VBD27_000558"
     CDS             9410..13351
                     /locus_tag="VBD27_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO7024593.1"
                     /translation="MGNIGDLVATATLDISPFMSNTRNLKTYMKTLDNSLKAVENSFN
                     GHGSRVKGLKAIYTETGGALKSYQELLKKQSQKYNELKENIGDVNKATASQKQALINA
                     KSAMMETAAKVSELQSRLKALGAETSVFTRFGNAAEQAGKKMKSFGDSVSGVGAAFTK
                     GVTAPIVAGAGYALKAAVDYEDAFAGVKKTVDEVKDSNGKVVYSYDMLSKGIRQMAKE
                     IPASTTEISHVAEAAGQLGIKTKDVLGFTRVMIDMGKSTNLSSEEAATALARFANITQ
                     LDPSKYSNLGSSIVELGNNFATTEKEIVEMGLRLAGTGKVVGLTDPQILGLATAMSSV
                     GIEAEAGGSAFSRVMQKINTQVLSGGEDLWKFAKIAGKSADEFAASWKKNPQEAIIDF
                     VKGLKRFKEEGKDVTAHLQDMDINSVREIDTLQRLAGAGDLLGDAFKSANKGFSENKA
                     LMNEANKRYETTASKLQMLKNQVNDVAIEFGGPLIDALRDGLEAGKPIIKGVADLAKA
                     FSSLDKEQQQQIIKWGLIAAATGPALSIFGKGVGVIGGTIQGLGKLSKTLGTLSGSLG
                     AAKTGAAALGVGAEGATTALTGMSGAAALLGNPVTWGVVLGGAAALAVGYFAQKAYEA
                     HQRTQEWGTKVSQVQANELQSFKDKVDQTNQSMEGFRGGAEQVNSVKTAFQGLVAEIE
                     KLENKDLSEKIKLAEQFGFSQGTMDQIKKSSRQTVENVKQMSDEVINIYQNASNEHRR
                     LTEEEKAVVLANQNELINVQLSKLNYSAKEKKAIVKAMNGDLNSLNSQQLTKALEVTE
                     KWIKAENKSYNKLKSGLKKVYDSIKGNDKEAVKTREEIHKKQQQLEADHYLKMEAYGK
                     RYAAIQKKLLKGTAKYLDPQLQQAMVNDVKKQMKELGLSYEELMTKTTKAASKAQEVN
                     TMWARTTKKSTEDQKLANSQWNGLVWNPKTGKLKTNAKEEVAKALEAEGGWDRLKFIA
                     KNANLETNARITMAEVLVETGKWDTLTPEEKELVVDGHQGIQAIVESKQHLEIWNSLP
                     EEVKRILGDNKDFLNKKGVATRTLENWNALKPDEKKLLAKNLTKKGKDEAQKTINSLV
                     GKEVKVTAANKTLSGVNSAQRTLNSVQDKHVTIWASIKKTASDLWSKLTGYAVGTDYH
                     PGGLAMVNDQKGSLYKELVTLPNGQSFIPDGRDVILPLPRGSKVMKASATRDYMYDLG
                     IPKYANGIGFDNTKIANITQRMSELPKNTVTSTTDDKLYWMIEEMIAVLKSTKDNSVI
                     EQALDIAEQAIERPVELYLQDGQWVAKVADRITNYQTQRNSRNNRMKGMLN"
     gene            13348..14871
                     /locus_tag="VBD27_000559"
     CDS             13348..14871
                     /locus_tag="VBD27_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO7024594.1"
                     /translation="MKFIYDGVDMSKFFRISRVERSIGNERTLSLNETFQLGTAIRGI
                     KTGAKIIKVHIEPLEINGVLTEQLKHELAGVLNVDKPKKMTFGDEPDKYYLGLAQGEI
                     STEKVARWYQRAVITFYIPDGVAHSITYKKFSDYTQEGNKLIFNLQNDGNTNAYPIIK
                     IKHNSENGYIGIANETGAFALGSSEEEDGTIVHRNESLFDYSKAIAQALEGAPNVAKL
                     NYMPPTYDTELKRMRIDNILGSGKGGEYVVIGNRGTTPGYTEHIGTRTFIINPDSNGE
                     YTLNEHLWWQQIFIATAQDQKGFLKLCVTGIDDEGNDEFLYGIETYKRKNGFETEYNF
                     FALDDDGVGWRFYKQFKFQADRNYHNPFSMNRSRAVEIFREEDKFRIYFNGAHHHVTV
                     PSLKGKKSRKIHLAMGTCSDSSKYINYNLFEKVNFEKMGVSHYNNIVNKYQPGDEVVI
                     NFENDTVKTKDLDSIQDMVLGSQPISIPPGKTEFVMQLSKFSQYAPNVEILMEERWL"
     gene            14862..18689
                     /locus_tag="VBD27_000560"
     CDS             14862..18689
                     /locus_tag="VBD27_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7024595.1"
                     /translation="MVIITLVIHDSKLHPVLLLDNEKQGTLNYFDDTWTRQLTTGSSV
                     FEFSVYKKTLEGDNPLNHKYQVLNDQAFVSFVHKDKVQLFNIMQIEETETTVRCYCEN
                     LNLELLNEYCNAYKATKAMSFEEYLVQFDILNWGALTIGTNEVKDKKLTLEWTGQDTK
                     LARILSIANNFDAEIEFETQLHNNHTFKAFIVNVYKEYEEGVSYGVGRDRSDIVLRYQ
                     KNVTGITKKLDKRQIYNAIRPYGKKTVKGERVISNPVTRKVTKTVGSNRTYLGGDLKY
                     YGHTIKKANVQSIINYAVQYNILPSGIICQLYLESLWGDSAVGKRDNNWAGISGGAQT
                     RPSGVKVTTGMARPPSEGGTYMHYASVDDFLKDYTYLLAKQGLYNVVGKKNIADYTKG
                     LFRVGGAKDDYAAAGYQHYISTMTSIRNGINKVSGNILNTIDTLWQTPVKPITSVTTA
                     KRATKTIQAINEATKLKGRRVGSGQCYALSGWYAKKLDGAWIDSSIGGIRGRIGGGMA
                     AALIGTDYNWGAYGWKVDKSPNARNLKAGGIYNVRANRGAPFYTTGWGHTGIIKSVSK
                     TRVTVLEQNFVGRMYVVENSYDINSFVSGLQTVCYPREIAQGMSVNGATTQQVTGGTQ
                     ISYEEVVQEAQTETYEEEQIIYIDNSIYKEWKDENGKVEYYLKNGFLYAPLSRDRYPS
                     VLTGNETRDNWIRKDMEVETDSQEVLMSTGLKDLKAHAYPAITYEVDGYVDLELGDVV
                     RIQDDGYEPPLILTARVVEQEISITNPSSNKTKFSNFVEKESQLASDLISDMLRLYDE
                     SIPYEIKLATSNGVAFKNGTGESVLTPSLQKNGKDYEAVYFYKNGDSLIDIGPSLIVK
                     ASDFNHVLNITVEAYLNEELVASTQISFTDTEDGEKGDDGATSWTAWANSKDGKVDFS
                     ITEAKNRRFIGTYTGLTQSTNYLDYKWIDMSANVVIGTQNLLDGTKSFSGSWFTEGTI
                     FETTKISEYPFEFKKWKSGNKVSHTIEFDVKAGVTYTFTAAIARENAGRLYFYLYDLF
                     ANHITSNTPRETIIENVTTDIQMFKVTFVPLRDGKIKPRFAMLASDAGWFMTGGYMLV
                     KGNKSGDWQESEVDRINNLDTKADQELTQAQILALEERTAIARENAIAEAMQNTLSEV
                     ETKWKLWYDLNTIDEKQKVANDIAQLFDRTTEFKQLLGEASARFSFINNETLIGEEGV
                     AIGDKGGKAKLFLSNDSISFVTNGVAQMTLTGDTLTIKNGLFTERIQIGNFVEEVYDR
                     NPLFNVIRAIRNS"
     gene            18700..>20387
                     /locus_tag="VBD27_000561"
     CDS             18700..>20387
                     /locus_tag="VBD27_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024596.1"
                     /translation="MGTATYSRSWGNNLTLEILSAWNKPNIASNTSTVNVQVFLKMSS
                     YGYISIGETRPLKITVDGRAETINVNPSINYGQRKLLFAKDYIVNHNSDGNKPLFNIS
                     AYYPINFSNYGEATANQSISLPKINRLSVSSAISGVLGNAVTITINRYSTSFTHNLKY
                     DFKGSTGTIATGVGTSYLWTIPPTFANLLPNELTGTGNLIVETMDGSAKIGETKYTLS
                     ITIPNTATYKPKLSSITLSDTNTLTSSIVSGNNFVRIISKVKVDFGSAIGNNGSTITS
                     YNAEIVGKSNSIIGNGSVFDKLDFFGSATIRATVTDSRGLTSEPVDTKINVIDYFLPI
                     VTSAKVVRSQQNPDILQVLPFVKIAPIIVGGIQKNQLKMSVSVAPYNTGIYAVDSGAA
                     TNTWSTISQMSGAPLNLGGTYDKSKSWLVKVSVSDSLMSAIPITQTISSEFVLVTKAP
                     SGVAFGKIWEHGIIDAKGDVYVDGTIYCGDKAIQQKPLALNNGGSFRHDDTDLNSLQD
                     TGFYCVFRGANRPAGAGPGYVTVVRHETANYAYQQFYDRTNKTIFTRVLENGVW"
BASE COUNT         7124 a         3341 c         4404 g         5518 t
ORIGIN      
        1 atcagcgtca gaggagacgc gacccagtat cccgaaaggc agtcacgcac tgatatactg
       61 gtttttactt ggttgatagt catattccta ccaatgacga ttggtaaaaa agatacatag
      121 atcggctgta tagtctacgt gtacgtattc acaacagatt gactcatttg attaaccaca
      181 agtaaaacaa gggtcgcaac cttgcttgtg gttagtaggg acaatggttg taagtcggtt
      241 cgattccgac tgttcctgtt cgataaatag aagtgtccca gaatggggta ggcaataggc
      301 ttagcattca tttgctattt atctatggtt aaccaattag tcatcacatt gtggtggctt
      361 tttattatgg aggttagggt atgaggccac agaagttaac catcgctggt ggtagacgta
      421 caacggttga ttatgatgat aggtcagcag agtatcgcga ctataatcgc aatcgctgga
      481 agtacgataa gcaagtcaaa cagttttata actctaagtt gtggagagag acaagtaagc
      541 aagtcttgtt gcaaaacgat tatgtctgtg ctatgtgcgg tggagaagcg actatgactg
      601 accacatagt ctcagttaag aaagattgga acagaagatt agattggaat aacttgcaag
      661 caagctgtaa agcgtgtaac gatagtaaag cgataagaga aagatgtaaa aataattaat
      721 tggaaaacgt gcgtaaaatt aacaacagca cgttaatgtt cggaaaatac cccgcttgtt
      781 tttgaacagg gctatatcgt tcgtgttttt gagaacgcgc ccttttccgt gcaaaatatt
      841 ccctttttga aatttttgaa ctgtcaattt tcgtgtaaag gaggtaactt tgggaagaaa
      901 attaaaggtg gttgaaacaa caaaaaaaca cctcacaaaa gaagaaaaaa tagcaagaga
      961 aaccgcgcag aataaggctt ctgacggact tgataaatta caaataacgc cccctcggca
     1021 tctcaatgag gtggctagag cggaatatcg tagaatcata aatgacctcc agaccctacc
     1081 cgtaagaaac ctcgatagag gccttctaga actctattgt tcttggtatg caatctataa
     1141 agagacaacc aagaaattag atgaaattgg ttattttaca aacgacccgg ataaagggtt
     1201 gattccaagt ccgctcattt taacattgga aaaagctact gcgaatatca gaagtagcgc
     1261 tagtcaatta gggctgacag tagatagtcg tatgaagatg tttataccta aagaagaaga
     1321 aaaacaaaaa agtatatttg ataaatttgg cggttagtta gaaaggaggt catgacaata
     1381 gaatacgatt attcagcaat tagcgacatt tataaagatg acacttttta ttatgcaaaa
     1441 aagattgttg atgaagagat taaagcaagc aagaaagtgt ttaaggcttg tttaagacat
     1501 ttgaatgacc tcaaacgtat agaagatgaa gattttaaat tcatctactt gccacaaaaa
     1561 gcatctgatc caattaattt cattgagatt ttaccagacg tcaaaacagg aaaaccttat
     1621 ccattagcaa tgttccaaaa atttattatt gggaatttat atggatggcg gaagaaaaca
     1681 gaccattcct taagacgttt tagaaaagct atgatttctg ttgctcgtaa aaatggtaaa
     1741 accattctta tagctggtat attgctttat gaatttttgt ttggtcacaa tccgtctatg
     1801 agtaggcaat tgttttgtac agcgaacgac cgcacgcaag ctaaaatagc ttgggatatg
     1861 gcaaaaaagc agttagcttc cttaagagca aaggatgccg atgtcagaaa agctacaaag
     1921 attgtccgtg atgaactaaa aaacttacat gatgaatctt atataagggc gcttagtcgt
     1981 gatactggcg cagttgatgg atttgaaccg tacgttggag tgttggatga gttcgcagcg
     2041 tcaaagacaa acgaaatgtt agaactattg gaatctggtc aaggacagtt ggataatccg
     2101 tttatcttaa tcatttcaac ggctggtatg gatttgaatg ttccgatgca cacaattgag
     2161 tatccataca ttactaaaat actagacgga gaaatcacag acgagggcta ttttggctat
     2221 gtcgcagagc aagacaacga ggaggaaatt aaagatgaaa cgaattggat aaaatctaat
     2281 ccaattctcg aagtcgatac tctacatgat aagttgatgg actatctaag aactcgtcgt
     2341 aaggtatctc tagagactgg agaagtcaac aaagtgttga tcaaaaactt caatatgtgg
     2401 cgtcaatcca gcgaagaatc atatatagat aaacagtcgt gggagcttgc taagattgat
     2461 aagccagaca catacaagcg tagggtttgg ctaggtgttg acgttgggcg tgtaagtgac
     2521 ttgtttgcca ttagtcctgt tgttatgatg gatgattatt ggtatgttga tagtttttca
     2581 tttgtagcta caaagtatgg cttaactgcc aaagaaaagc gagatggtgt atcttatagc
     2641 aatctagaac gtcaaggata ttgcgaaata acaacccttg agagcggggt tatagatgat
     2701 gaacgggttt tggaaaaaat agaggagtta atctatataa acgaatggga agtacatggg
     2761 atttgctttg acccatacca attcggaaca ctacttacaa tgattgaaaa aagacatccg
     2821 gaatggcctc taatagaagt ttcgcaaacg acaatggtgt taaacatgcc gacaaaacaa
     2881 tttcgtgacg accttaaaaa aggcaaaata aagcattctg gcaatccact attgaccatg
     2941 gctgctaaca acgcttatat taaaaccgat aacaatggca tgaggattga caagaacaag
     3001 aatagcaaca agattgaccc gctagacgca gttcttgacg gttatgctgt atgttaccta
     3061 gaaccatttg acggttctgg ctactggaca agcgagaaaa ttttaggagg agagacgcta
     3121 ttttgattgg ttttatttta aaaaacatac atacattgat cttgctggta ggtttgggac
     3181 tgttgatata cggtcttttt ttatttggtg ataaggttgg ttttattgcc agcggaatta
     3241 tattagttat tttagctatc tatgtagata gtgtaggggg aagaaaatga acaaacgtat
     3301 taagaaaaaa cgcaaactag aacactatat cgcttcactc atcgcagaaa acgttatgtt
     3361 ttcaaaggaa ttaatcaaac aacatgaacg gattgaacaa cttgaaaaaa ttgtagagca
     3421 taacgcacaa gcgactaaca atgaacttag tcgcatcaaa aaacactcaa agaaaaaatg
     3481 gaaaaataac gtcaccttac ctatagaaag gaggtgagaa tctatgagtt tttttcaacc
     3541 tttgggaact tcaaaggtat cgtatgacga ttatatagcg tcagtcttag ctggtgacgt
     3601 ctcacagcag tatctaggtg tatcagcctt aaaaaatagc gatgtcttga cagctacgtc
     3661 tattatagct ggtgatatag ctcgttttcc gctcgttaaa aaggatataa acggggatat
     3721 tatccacgat gaggatatta actacctatt aaatgtaaag tccacaaaca acgcaagtgc
     3781 taggacatgg aagtttgcca tggctgttaa tgcaatttta acaggtaatt cgttttctcg
     3841 tatcttgcga gaccctaaaa ctggtcaagc tttacaattt cagttttata gaccgtctga
     3901 aactactgtt gaagaaacag aaaaccacga aatcatctat acatttacgg atactttgac
     3961 tgctaaagag ataaaatgtt ttgcacacga tgttattcac tggaaattct tcagccatga
     4021 cacaatactc ggaaggtctc cgctattatc gctaggcgat gagatagact tacaaacggg
     4081 tggtatcaat accttaatta aattcttcaa agatggtttt tctggtggta tcttaactat
     4141 gaaaggtgct caattaagtg gagaagcgcg tcaaagagca agacaagact ttgagaaaat
     4201 gcgtgaggga tctgttggag ggagtccact agtttttgac agcacaatgg aatatacgcc
     4261 acttgaaatt gatactaacg tattgcaatt aatcacaagt aataacttta gtacggctca
     4321 aatagcaaaa gcgttgcgcg tgccaagtta taagttgggc gtaaacagtc ctaatcagtc
     4381 tgtagcgcaa ttaatggaag attacgtaac aaatgacttg ccattctatt ttgacgctat
     4441 aacgagcgaa atagggctta aaacgcttaa cgatggagac aggcgactgt atcatgttga
     4501 attcgataca cgaagcgtca cgggtcgcaa tgtcgatgaa attgtcaaat tggttaataa
     4561 ccaaattttg acacctaatc aaggattggt tgaacttggt aagcaaaaat caaaagaccc
     4621 taacatggat aggtaccagt ctagtctcaa ttacgtgttc ttggataaaa aagaagaata
     4681 tcaagatcaa gttggagtca aagggaaaag gggtgaggta aatgccaaag aaaataaacc
     4741 ttaaaggtcc actaattgct aataattcac aagaagtcta tgactattat ggcatggagg
     4801 ctgttagtgc taaaagcatt attgacaaat tgccagaaga taatagcgac attatcttgg
     4861 aagttaattc aaatggtggt ctcgttactg ttggtagcga aatatatacc gctctgcgaa
     4921 attacaaagg gaaagtaacc gcagaaatca caggaatggc tgcgagtgca gcgtctgttg
     4981 cagtgatggg ggctgataaa gtcgtcatga gcccaacggc tcaaatgatg gtacataaag
     5041 ctttgtttaa ttgggtttct ggtaatagcg acgacttgga caaagcttct agcgctctta
     5101 aatcaagcga taaagccatt gttaatgctt atgttgcaaa aacaggtaaa tcagaagatg
     5161 aaatcatgca attaatgcgg aatgaaactt ttatgtcagc acaagatgct gttgagagcg
     5221 gatttgcaga tgaagtgatg tcgtttgaag ctatagcaag cattgacaat caaatgttgc
     5281 cacaagcggt tattgatgac tattacgcaa gcagaagcaa acgtaagcaa gagattaata
     5341 acatgttgct agaaatcgaa aaagaagaaa ttttacaagg gctataagct ctttttattg
     5401 gaggaagaaa atgtttgacg aaaaaatcga agaaattaaa gcggatatca ctgaattaaa
     5461 caaagtgatt gctgctaaaa ccgcagaagt caaagctgct ttagaatcag atgacttaga
     5521 aactgctcgt tctgttaaag cagaggtaga agctactaaa gcgaaattag ttgaatcaga
     5581 aaacgacttg aaattatatg agtctagcgt tgaagttggt ggtgctgaaa atattggagg
     5641 aaaagaagtg acacaagagg aaaaaacata tcgcgaaagt gtgaatgatt tcattcgttc
     5701 gaaaggtaaa atcgtaaacg attctttacg ttttgaaggc aaagatgagg tacttatgcc
     5761 tattaatgag acaacccccg tagaacctaa aaaagacggt gttaagaaaa cagatgtaaa
     5821 acctgtctct agtgaagaaa tcctttacac accagctcgt gaagttaaga cagttgttga
     5881 tttgaaacaa ttcacaagca tccaccaagc taaaaaagca tctgggaaat ggccagtttt
     5941 gcaacgtgcg actgaaaaaa tggttagcgt tgaagaatta gaaaaaaatc caaaactagg
     6001 taagccacaa ttcacaaacg tcgcatggga aattaaaact taccgcggag ctatcccgtt
     6061 gtctcaagaa tcaattgacg atgcagacgt agacttggtt ggtattgttg ctgagacaat
     6121 cggtcaaatg aaagttaata caacaaacga cgctatcgca acagttctca aggaatttga
     6181 agctaagaca gtaaaaaact tggatgaaat caagaagctt ctcaacgttg aattagatcc
     6241 tgcttacaat gtgtcattta ttgtatctca aagcttttat caaacaatgg atacattaaa
     6301 agataagaat ggtcgttacc tacttcaaga ttcaatcact tctgtttctg ggaaagtatt
     6361 cctcggaaaa cctgtttttg tgctagctga tgaggtttta ggtaaagata cagcatttgt
     6421 cggagatttc aaacgtggtg tattattcgc cgaccgcaaa gaccttggac ttcgttgggc
     6481 agataacgaa atctacggtc aatacttgca agcagttctt cgctttggcg tttcaaaagt
     6541 tgatgacaaa gctggctact atgtgacatt caaaccagaa caattgccct cataaggcca
     6601 ctcagggagt ggcgcaacca gaagtaagca gtgctacagt tgttggtaaa ccaaccaaag
     6661 acagcaccat tgaagatatt aaaaaatatc taacaagcca aaatattgac tttagcggta
     6721 agacgctaaa agcagacttg ttaaaactgg ctggcgttga agaggtatag ttatgactgt
     6781 atctaaagag ctgctagata gtgtgaaact ctattgtaag atagattttg attttgaaga
     6841 tgacattatt gaggaaatga ttgcatcagc acaagaacaa atatgttttg caatagaaga
     6901 aggctctact ccagaaatgt ttaaaaacta cgctaagttt tccttagcgg ttaaaaaaca
     6961 ggtaaaggaa gagtatgatc atcgtgggtt atcggcggat actaatcgtt atccgcttgc
     7021 caacggtgtt ttgaacatta tccatcaatt gagattgagg ggtgatgggg cgtgattaca
     7081 cggaaaatga atgttagaat agccattttt agccaaactg gtggtcagaa tgaagatggt
     7141 gaagttgtat ctgctatcag gaaagacttg tatacctgct gggcagaggt gtctaaaact
     7201 caattgagag attttaatta tcaatcaaga tatcagaatg ctagcaatct cccaacaaat
     7261 aaagatacaa aagtattttt aattagatac aatcctaagt tgtctattga taatacgatg
     7321 tttattgagc ataacaaaca tatctacaaa atagataaaa ttgaatttga tgaatccggc
     7381 aaggaaatga taatattgag tggggttagc atatcatgac aaaagggctt gatgaaattt
     7441 tggctaatat tactaagtta gaagtaaaat cgcccaaaat tgccaaagaa gcggtaacac
     7501 aggttgctga agaatttgaa aaagcactta ctaagaatac gccagtcgat tattctgtgc
     7561 atgacacaaa actcaaatat gatactgcaa ttagtggatt caaaggtgct aatgtaggta
     7621 taacgtcaaa aaaaattgga tatggacgaa aaacgggctg gcgtgctcat tatccaaaca
     7681 atgggacgat ttaccaaaaa ggtcaagact ttaaagaaaa aaccatcaac gaaatgaccc
     7741 ctagagcaaa agaaatttat gctaaaaaaa tcaaggaggg actagggctt tgatagctga
     7801 aacaactgct tacaaattgt taaaaaacga taggacgctt aatgagctgt tggataagct
     7861 tagaggcggt ccttttaaaa acggatttga tcagggtatc tttacatatg atattcctga
     7921 caatcccgtt gatttaagaa aagtagaact agcgccattt atgcgtataa atacaacgta
     7981 tgatggtccg tccctatatg cagatgatga catgatcagt aatgaacagc gtattacaat
     8041 caatttttgg tgtaaaaccg cttctcagtc tgaccagatt gccagctgta tagataggat
     8101 tttaaaaaat ggcggttttg aaagatacac cgcaaacgaa aaaccaaggt ataaagatag
     8161 cgatattgac ttactcatga atgtaaggaa ataccgctat tttgattttt attaagaaaa
     8221 gaggaactaa atgggaaaag taaaatttgg actacgtgac ttccagtatg ctgtgcttgg
     8281 ggataatgat aaagttaatg aaaaagacaa aggaatcaaa gctctacccg gcatgaaatc
     8341 agctaaactt gatattacaa acgagcttgt aactgtcatg gctgatgatg gaccatatgt
     8401 tgttttatct ggtggtatca cagaaacaaa attggaaatc gaagtccttg acttgacttc
     8461 cgaagcgcgt caagatttct tcggaattaa agctgacaaa ggtattgaaa gatacaataa
     8521 atcgctcaca cctaacaacg tcgcttgcat gtttagaaca agtgatgaaa atgggaaagc
     8581 tatctgggtt ggtttgttaa aaggtaaatt taacattcct ggcatggata ccaaaacaaa
     8641 agaaggtgcg cctaacccag aaccagacaa agtaacaggt aactttgttg ctcgtggaac
     8701 tgatggtgat gtagttgtca tcggtcgtga aggagtgaat ggatttgagc taaaaaaatt
     8761 caaagaactt gtgtttgatg gggtgacagg attggaaaaa tcatcagaag acaatagtct
     8821 aatgtaacac ttaaggttgg gttttaaatc cagcctttat tttttgataa ggagcaataa
     8881 tgtacgaaat tacacttaaa aaaggcggag tagataagac tttctcaaaa gattttgtca
     8941 atgtcgaaga taatatgttg gcggttgagc atcaagttag acagagcgct atctttagtg
     9001 atgataagcg ccggttagac gataaagaac atcgcaaatt aaacgaatca tatttacaga
     9061 tgtttgtaga aatgtacgga aatcaattca atgttgacga tttgaaacag tctaatgtgt
     9121 ctgttttgga caaattaact gagctttttg ttgatgcttt aggcggagag aaagaggaag
     9181 acgaaaaaaa ggaacaataa cgcctcaaga agctaaagac aacctgctct tgtggattca
     9241 aagtttacta aaaaatgggt ataccatttt agatattaaa aaaatgcgct tatcagacat
     9301 agagttgatg gtaaaagcgc tagaaactga ttttgctgaa aaagaagaag tggttgaaac
     9361 aactttagac aaagcgttcc cattcctttt cggctagaaa ggagaattaa tggggaatat
     9421 aggtgattta gtagcgactg ctacactaga catatcacct tttatgtcta atacaagaaa
     9481 tttaaaaact tatatgaaga ccttagataa ctctctaaag gctgtagaaa atagttttaa
     9541 tggtcatggt agtagagtta aaggtttaaa agctatctac acagaaactg gcggtgcatt
     9601 aaaaagttat caagaattac taaaaaaaca atctcaaaaa tataatgagt taaaagaaaa
     9661 tattggcgat gtcaacaagg cgacagcaag ccaaaaacaa gcacttatta atgctaagtc
     9721 agcgatgatg gaaacagctg ctaaagtttc agaattgcaa agtaggctca aagctttagg
     9781 cgctgaaaca agtgttttta ctcgttttgg taacgctgcg gagcaagctg ggaaaaaaat
     9841 gaagtcattt ggcgattctg tatctggtgt tggtgctgct tttacaaaag gagtaactgc
     9901 accaattgtt gctggcgctg gatatgctct taaggctgct gttgattatg aagatgcttt
     9961 tgctggcgtc aaaaagacag tagacgaagt aaaagactca aatggtaaag ttgtctactc
    10021 ttacgatatg ttgtccaaag gaattagaca aatggctaaa gagataccag cttctacaac
    10081 tgaaatttct cacgttgctg aagcagctgg tcaattaggt atcaagacaa aggatgtctt
    10141 aggatttact cgtgtcatga ttgatatggg gaaatcgact aacttatcat cagaagaagc
    10201 ggcaactgca ctagctcgtt ttgctaatat cacgcagttg gacccatcaa aatacagtaa
    10261 tctaggaagt tctatcgttg agttaggaaa taactttgcg acaactgaaa aagaaattgt
    10321 tgaaatgggt cttcgcttag ctggcactgg taaagttgta ggattgacag accctcaaat
    10381 attaggtctt gcaactgcta tgagttcggt tggtatcgag gcagaggctg gtggttcggc
    10441 gtttagtcgt gtcatgcaaa aaataaacac acaggttctt tctggtggag aagatttatg
    10501 gaagtttgcg aaaatagccg gcaagtctgc tgatgaattc gctgcgtcat ggaagaaaaa
    10561 tccacaagaa gcgattattg attttgtaaa aggtcttaaa cgctttaaag aagaagggaa
    10621 agacgtaact gctcacttgc aagacatgga tatcaattct gtacgtgaaa ttgatacatt
    10681 acaacgtttg gcaggagctg gcgacttgct aggtgatgct ttcaagtctg ctaataaagg
    10741 ttttagcgaa aacaaagctt taatgaacga agcgaacaaa cgttatgaaa cgacagcatc
    10801 gaaattacaa atgctaaaaa atcaagttaa tgatgtcgct attgaatttg gtggacctct
    10861 tattgatgca ctgcgtgacg gtttagaagc tggtaaacct attattaaag gcgttgcaga
    10921 cttagcaaaa gcatttagtt ctctggataa agagcaacaa caacaaatca taaaatgggg
    10981 actaattgca gcagcaactg gacccgcatt gtcaatcttc ggcaaaggcg ttggcgtaat
    11041 tggtggaacg attcaaggac taggaaaatt aagcaaaacg ttaggcacac tctccgggag
    11101 tctgggagca gcaaaaactg gagctgctgc tttaggagtt ggagcggaag gtgcaactac
    11161 tgcattaact ggaatgagtg gagcggcagc attgctaggg aatcccgtaa cttggggtgt
    11221 tgtgctagga ggagctgctg cgcttgctgt cggatacttt gctcaaaaag cctacgaagc
    11281 gcaccaacgt acgcaagagt gggggactaa agttagtcaa gtacaagcca atgaactgca
    11341 atcctttaaa gataaggttg atcaaacaaa tcaatcaatg gaaggcttta gagggggagc
    11401 tgaacaggtt aactccgtga agacagcgtt ccaaggttta gttgctgaaa ttgaaaagct
    11461 agaaaataaa gatttaagcg aaaaaatcaa acttgcagaa caatttggtt ttagccaagg
    11521 tacgatggac caaataaaaa aatctagccg acaaactgta gagaatgtta aacagatgtc
    11581 tgatgaagtt atcaatatct atcaaaatgc tagtaatgaa cacagaagat taaccgaaga
    11641 agaaaaagct gttgtattag cgaatcaaaa cgagcttatc aatgtgcaac tgtcaaaatt
    11701 gaactactct gctaaagaga aaaaagctat cgtaaaagca atgaatggcg atttaaattc
    11761 attgaatagc cagcaattaa ccaaagctct tgaagttact gaaaaatgga taaaagcaga
    11821 aaataagtca tacaataaac tcaaaagtgg tcttaaaaaa gtttacgact ctataaaagg
    11881 taatgacaaa gaggctgtca aaactcgcga agagatacac aagaagcaac aacaacttga
    11941 agcagaccat tacttaaaaa tggaagctta cggcaagcgc tacgctgcaa ttcaaaagaa
    12001 attgctaaaa ggtactgcta aatatttaga ccctcaattg caacaagcga tggttaatga
    12061 tgttaaaaag caaatgaagg agcttggttt gtcttacgaa gagttgatga caaagacaac
    12121 caaagcggct tcaaaagcgc aagaagtgaa tacaatgtgg gctagaacca ctaagaaatc
    12181 tacagaagac caaaagcttg ctaattcgca gtggaacgga cttgtctgga atcccaaaac
    12241 aggtaagtta aaaaccaatg ctaaagagga agtagccaag gctcttgaag cggaaggtgg
    12301 ctgggacaga cttaagttta ttgctaaaaa cgcaaaccta gaaacaaacg ctcggataac
    12361 catggcagaa gtacttgtag agactggcaa atgggatacc cttactccag aagagaaaga
    12421 gttagttgtt gatgggcatc aaggtattca agctatagta gaaagtaagc aacatttaga
    12481 aatatggaat agtttgccag aagaagttaa gcggatttta ggggataaca aagacttcct
    12541 gaataaaaaa ggagttgcaa cacgaactct tgaaaattgg aatgctttaa aaccagacga
    12601 aaaaaaatta ctcgctaaaa acttaacaaa aaaagggaaa gatgaagcac aaaaaacgat
    12661 caatagctta gtcggaaaag aagtcaaagt gacagcggct aataaaacat tgtccggagt
    12721 taacagcgct caaagaacat taaatagtgt gcaagataaa catgtcacta tttgggcgtc
    12781 aatcaaaaaa actgcgagtg acttatggag caaactaaca ggatacgctg ttggtacaga
    12841 ctaccaccct ggtggacttg cgatggtcaa cgaccaaaaa ggttctttat ataaagagtt
    12901 agtaacatta ccaaacggac aatcttttat cccagatgga cgtgacgtta tcttgccact
    12961 ccctcgtggt tcgaaagtca tgaaagcaag tgctactcgt gattacatgt atgacttagg
    13021 aataccaaaa tatgcaaacg gtattggttt tgacaacact aagattgcta atatcacgca
    13081 acgaatgagc gaacttccta aaaacacagt gacttcaaca acagatgata agttgtattg
    13141 gatgattgag gaaatgattg cagttttaaa atctactaaa gataacagcg ttattgagca
    13201 agctttagac attgcagaac aagcaataga gcgtccagta gaactttact tacaggacgg
    13261 tcagtgggta gctaaagttg cggaccgcat tacaaattat caaacgcaac gaaatagtcg
    13321 caataatcga atgaaaggaa tgctaaattg aaatttattt atgacggtgt agacatgtct
    13381 aaatttttta ggatatctcg tgttgagcgg tctataggaa atgaacgaac attgtcgttg
    13441 aatgagacgt ttcaacttgg tacagctatt cgtggcataa agacgggcgc taaaattatc
    13501 aaagtacata tagagccact agaaataaat ggcgttttaa ccgagcaatt aaaacatgaa
    13561 cttgctggtg tgttaaatgt tgacaagcct aaaaaaatga cgttcggaga tgaaccagat
    13621 aagtactatc tcgggcttgc acaaggtgag atttcgacag aaaaagtagc aaggtggtat
    13681 cagagggctg ttatcacttt ttacataccc gacggtgttg cacactctat cacttataaa
    13741 aagttttcgg actatacgca agaaggcaac aagcttattt ttaatttgca aaatgatggt
    13801 aatactaacg cttatccaat aatcaaaatt aaacataact ctgaaaacgg ttatatcggt
    13861 atcgcaaatg aaacgggcgc ttttgcactt ggatcatcgg aggaagaaga tgggactatc
    13921 gttcatcgca acgaatccct ttttgattac tcaaaagcta ttgcacaagc tttagaaggt
    13981 gcgcccaacg tcgcaaaact caactatatg ccaccaacat atgacacaga acttaaacgg
    14041 atgcgcattg ataatatctt aggctctggt aaaggcggtg aatatgttgt tattggaaat
    14101 agagggacta ccccgggcta caccgagcac attgggactc gaacgtttat tatcaatcct
    14161 gattcaaacg gagaatacac tctcaatgag cacctatggt ggcaacagat ttttattgct
    14221 actgcgcagg atcagaaagg gtttttaaag ctttgtgtaa cgggaatcga cgatgaagga
    14281 aatgacgagt ttttgtatgg aatcgaaact tacaaacgaa aaaatggttt tgaaacagaa
    14341 tacaattttt ttgctcttga tgatgacggt gtaggctgga gattttataa gcagtttaaa
    14401 ttccaagcag acagaaatta tcacaatcct ttttcaatga atagaagtag agcggttgag
    14461 attttcaggg aagaagacaa gtttcgtatt tattttaacg gtgcgcatca tcatgtaact
    14521 gttccatctc ttaaaggaaa aaaatctcgc aagatacatc ttgcaatggg gacatgtagt
    14581 gatagctcta aatatatcaa ctacaatctt ttcgaaaagg tcaactttga aaaaatggga
    14641 gtgtctcact acaacaacat tgttaacaag tatcaaccag gcgatgaagt ggttatcaat
    14701 tttgaaaatg acacagtcaa aacaaaagac ttagattcta ttcaagacat ggttttaggt
    14761 tcgcagccta tatctatacc acccggaaaa acagagttcg tcatgcagct atctaaattt
    14821 tctcaatatg cgccaaacgt tgaaatatta atggaagaga ggtggttata ataacgctag
    14881 taatacatga ttctaagttg caccctgttt tgttgctaga caatgaaaaa caaggtacgc
    14941 ttaattattt tgacgacaca tggactagac agttaactac aggctcatct gtttttgagt
    15001 tttctgtgta caaaaaaaca cttgaaggag acaacccact caatcataag tatcaagtgc
    15061 ttaatgacca agcgtttgta tcgtttgtac ataaagataa agtacagctc tttaatatca
    15121 tgcagatcga agagactgaa acgactgtac gttgctattg cgaaaatctt aatttagagt
    15181 tactaaacga gtattgcaac gcatataaag caactaaagc aatgtcattt gaagagtatc
    15241 ttgtgcagtt tgatatttta aattggggtg ctttgacaat tggcacaaac gaagttaagg
    15301 acaaaaaact gacattggaa tggactggtc aagacactaa gttagctcgt attttgtcaa
    15361 ttgctaataa ttttgatgca gaaatcgaat ttgaaactca attacacaac aatcacacgt
    15421 ttaaagcgtt tattgtaaat gtgtacaagg aatacgaaga gggcgtgtcc tatggcgtag
    15481 gtcgtgaccg cagcgacata gtgttgagat atcaaaaaaa tgtaactggt atcactaaaa
    15541 aattagacaa gcgccagatt tacaacgcca tacgcccgta tggcaaaaag acagtcaaag
    15601 gcgagcgcgt tatttctaat cctgtaactc gcaaagtcac taaaacagtt gggtcaaatc
    15661 gtacatattt aggcggagac ctcaaatatt atggtcatac aatcaaaaaa gctaacgtac
    15721 aatctattat taactacgcg gtgcagtata atattttgcc gagtggaatc atatgtcaac
    15781 tgtacttaga aagtctttgg ggtgattcag cagttggtaa acgtgacaat aactgggcag
    15841 gtataagcgg cggagcacag acacgcccta gtggagtaaa agtcactact ggaatggctc
    15901 gtcctcccag cgagggtgga acatacatgc actacgcaag tgttgatgac tttttaaaag
    15961 attatactta tcttttagct aaacaaggac tatataacgt tgttggcaaa aagaatatag
    16021 cagactatac aaaaggtttg tttcgtgtcg gtggcgctaa agatgattac gcggcagcag
    16081 gatatcaaca ttacatatca accatgacct caatacgcaa tgggataaat aaagttagcg
    16141 gaaatatctt aaacactatt gatactttgt ggcagactcc tgtaaagcca ataacgtcag
    16201 taacaactgc gaaaagagct actaaaacaa tacaagctat taatgaggct actaagttga
    16261 aagggcgcag agttggttct gggcagtgtt atgcgctgtc tgggtggtat gcgaaaaaat
    16321 tggatggcgc ttggattgac agctctattg gtggtattcg tggtcggatt ggcggtggta
    16381 tggctgctgc cttaatcggc actgattata attggggtgc atatgggtgg aaggtagata
    16441 aatcacctaa cgctagaaac ttaaaagctg gtggtattta taatgtacga gcaaatcgag
    16501 gcgctccttt ttataccaca ggctgggggc atacaggtat tatcaagagt gtgtccaaga
    16561 ccagagttac tgttttggag caaaactttg ttggccgcat gtatgttgtc gaaaactcat
    16621 atgacattaa ctctttcgtg tctggattac aaacagtatg ttaccctcgt gaaatagcgc
    16681 aaggtatgtc tgtcaatggt gcaactactc agcaagttac tggtggaaca cagatatcgt
    16741 acgaagaagt tgtacaagag gcgcaaacag aaacatatga agaagaacaa atcatctata
    16801 ttgacaactc tatctacaaa gagtggaaag atgaaaacgg taaagtagag tactatctca
    16861 aaaatggatt tttgtacgca ccactttcaa gagaccgcta tccatctgtt ttaaccggta
    16921 atgagacacg agacaactgg atacgaaaag acatggaagt cgagactgat agtcaagaag
    16981 tcttgatgtc aacaggtcta aaagacttaa aagcacacgc atatccagca attacatacg
    17041 aagttgatgg ctatgttgac ttagaacttg gtgatgttgt gcggatacag gacgacggat
    17101 acgagccacc gctgattttg acagcacgag tagttgagca agaaatatcc ataacaaatc
    17161 ccagctctaa caaaactaaa ttcagcaatt ttgtcgaaaa agaaagtcag ttagcttctg
    17221 atttaatcag tgatatgttg cgtctatacg atgagtcaat tccatacgaa atcaaactag
    17281 ctacttcgaa tggtgtcgct tttaaaaatg gcactggtga atctgtccta actcctagct
    17341 tgcaaaagaa cgggaaagac tatgaagcag tttattttta taaaaatggt gactcgctaa
    17401 ttgatatcgg accatcgcta attgttaaag caagcgactt taaccacgtt ttaaatataa
    17461 cagttgaggc atatttaaat gaggaacttg tagcaagtac gcaaatatcc tttacagata
    17521 ctgaggatgg agaaaaaggc gatgatggtg ctacatcatg gacagcgtgg gccaattcga
    17581 aagatggaaa agttgacttt agtattactg aagctaaaaa tagaagattt atcggtactt
    17641 atactggatt aacgcaatca acaaattatc ttgactacaa gtggattgat atgtctgcta
    17701 atgttgtcat tggtactcaa aatttacttg atggtacaaa atcattttct ggaagttggt
    17761 ttaccgaagg tacaatattt gagactacaa aaatcagcga atatccattt gaatttaaga
    17821 aatggaagtc tggaaataag gttagtcaca ctatcgagtt tgatgttaaa gctggtgtaa
    17881 catacacttt tacagctgct atagcaagag aaaatgctgg aagattgtac ttctatttgt
    17941 atgacttgtt tgcaaaccat atcacaagta acacacctcg tgagacgata attgaaaatg
    18001 tcactacaga tatccagatg tttaaagtta catttgtacc gctcagagac ggtaaaataa
    18061 aaccacgctt tgccatgctt gccagtgatg caggttggtt tatgactggt ggatatatgc
    18121 ttgtcaaagg taataaatct ggagattggc aagagtccga agttgataga ataaacaatc
    18181 tcgacacaaa agctgatcaa gaattaaccc aagcacaaat tctagctctt gaagaaagaa
    18241 ctgctatagc aagagaaaat gcaattgctg aggctatgca gaatacactc agtgaagttg
    18301 aaactaagtg gaagctttgg tatgacttaa atacgataga cgaaaagcaa aaagttgcaa
    18361 acgacatcgc tcaattgttt gatcgtacaa ctgagtttaa acaactatta ggtgaggcaa
    18421 gtgcaagatt tagctttatc aacaatgaaa cgttgattgg tgaagagggc gttgctatcg
    18481 gtgacaaagg cggaaaagca aagttatttc tatcaaatga cagcatttca tttgtgacaa
    18541 atggtgttgc tcagatgaca ttgacaggtg ataccttaac aataaaaaat ggactgttta
    18601 cagagcgtat acaaattgga aattttgttg aagaagtcta tgacagaaat ccattattta
    18661 atgttatcag agcaattaga aatagttagg aggtgagaca tgggaactgc tacatatagt
    18721 aggtcgtggg ggaataacct gacacttgaa atattgtctg cttggaataa gccaaatatc
    18781 gcaagtaata caagcacagt caatgtacaa gtttttttaa aaatgtctag ttatggctat
    18841 atttcaatag gtgaaactag acctttaaaa ataacagttg atggtagagc tgagaccatc
    18901 aatgttaatc catcgataaa ttacggacag agaaaactat tatttgctaa agattacatt
    18961 gttaatcata attcagatgg aaataaacca ctattcaata tttcagcata ttatccaata
    19021 aactttagca attatggtga agcgactgca aatcagtcta tctcgctacc taaaattaat
    19081 agacttagtg tatcaagtgc tattagtggt gtgctaggta atgcagtaac tatcacaatc
    19141 aatagatatt caacgtcatt tactcacaat ttgaaatatg attttaaggg tagtacaggt
    19201 actatcgcaa ctggcgttgg tactagctat ttgtggacta taccgccaac gtttgctaat
    19261 ttactgccta atgaattaac tggtacaggt aatctgattg ttgagacgat ggatggatca
    19321 gcaaagattg gtgagacaaa atatacttta tcaataacaa tacctaatac agctacttat
    19381 aagccaaaat tgtcaagtat cactctatct gatacaaata ctttaactag tagcattgtt
    19441 agtggaaaca attttgttag gattataagt aaagttaaag ttgattttgg ctcagctatt
    19501 ggaaacaacg gttcaacaat aacaagttat aatgctgaaa ttgtcgggaa aagtaactca
    19561 attatcggta atggtagcgt atttgataaa ttggactttt ttggttcagc aacaatcaga
    19621 gcaacggtaa ctgatagcag aggtctaaca tcagaaccag ttgacacaaa aattaatgtc
    19681 attgattatt ttttaccaat tgttacaagt gcaaaagtag tcaggtctca gcaaaatcct
    19741 gacattttac aagtcttgcc atttgttaag attgcaccaa ttatagttgg tggaatacaa
    19801 aaaaaccaac tcaaaatgtc ggtatctgtt gcaccataca atactggtat ctatgcagtt
    19861 gatagtggcg cagctacaaa tacctggtca acaatttccc aaatgtcagg cgccccttta
    19921 aatcttggcg gcacttatga caaatcaaaa tcttggcttg ttaaagtatc tgtcagtgat
    19981 agtttaatgt cagcaatccc tattactcaa acgatttcta gtgagtttgt tctagtaact
    20041 aaagcacctt ctggtgttgc atttgggaaa atttgggaac atggcattat tgatgccaaa
    20101 ggcgatgttt atgttgacgg tactatttat tgtggcgata aggcgataca gcaaaaacca
    20161 cttgctttaa ataatggtgg ctcttttaga catgacgaca ccgacctaaa tagcttgcaa
    20221 gacacaggtt tttattgtgt atttagaggt gctaatagac cggctggggc aggccctggc
    20281 tatgtaactg ttgtaagaca tgagacagca aactatgctt atcaacaatt ttacgatcga
    20341 acaaataaaa ctatttttac cagagtgctg gaaaacggtg tttggag
//
LOCUS       DATRQA010000021        20268 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.021, whole genome shotgun sequence.
ACCESSION   DATRQA010000021 DATRQA010000000
VERSION     DATRQA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20268
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.021"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            71..2356
                     /locus_tag="VBD27_000562"
     CDS             71..2356
                     /locus_tag="VBD27_000562"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEO7024597.1"
                     /translation="MRYNQFSYIPTKPNEAFEELKGLGFPLNKKNSDKANLEAFLRHS
                     FLNQTDTDYALSLLIVDAKTDALTFFKSNSDLTLENLQWIYLQLLGFIPFVDFKDPKA
                     FLQDINFPVSYDNIFQSLHHLLACRGKSGNTLIDQLVADGLLHADNHYHFFNGKSLAT
                     FNTNQLIREVVYVETSLDTMSSGEHDLVKVNIIRPTTEHTIPTMMTASPYHQGINDPA
                     ADQKTYQMEGALAVKQPKHIQVDTKPFKEEVKHPSKLPISPATESFTHIDSYSLNDYF
                     LSRGFANIYVSGVGTAGSTGFMTSGDYQQIQSFKAVIDWLNGKVTAFTSHKRDKQVKA
                     NWSNGLVATTGKSYLGTMSTGLATTGVEGLKVIIAEAAISTWYDYYRENGLVCSPGGY
                     PGEDLDVLTELTYSRNLLAGDYIKNNDCYQALLNEQSKAIDRQSGDYNQYWHDRNYLT
                     HVNNVKSRVVYTHGLQDWNVKPRHVYKVFNALPQTIKKHLFLHQGQHVYMHNWQSIDF
                     RESMNALLSQELLGIDNHFQLEEVIWQDNTTEQTWQVLDAFGGNHQEQIGLGDSKKLI
                     DNHYDKEAFDTYCKDFNVFKNDLFKGNNKTNQITINLPLKKNYLLNGQCKLHLRVKTS
                     DKKAILSAQILDYGPKKRFKDTPTITFLNSLDNGKNFAREALRELPFTKDHYRVISKG
                     VLNLQNRTDLLTIEAIEPEQWFDIEFSLQPSIYQLSKGDNLRIILYTTDFEHTIRDNA
                     SYSITVDLSQSYLTIPTNQGN"
     gene            2360..2719
                     /locus_tag="VBD27_000563"
     CDS             2360..2719
                     /locus_tag="VBD27_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024598.1"
                     /translation="MKLLTKERFDDSQHFWYQINLLQESNFGAVFDHDNKNIPQVVAT
                     IVDDLQGSGSSNYFWYFGNTTDTSILMIAHLNRKFYIQVNLKNFDFALNLIAINNWKS
                     LLQTQLEALNDTLAIFQ"
     gene            2765..3745
                     /locus_tag="VBD27_000564"
     CDS             2765..3745
                     /locus_tag="VBD27_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO7024599.1"
                     /translation="MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAG
                     GKQLRPAFFYLFSQLGNKENQDTQQLKKIAASLEILHVATLIHDDVIDDSPLRRGNMT
                     IQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMRINAKSMRKILMGELDQMHLRYN
                     QQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDI
                     LDYTADKKTFNKPVLEDLAQGIYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAY
                     LVVSHRGVDKARHLARKFTEKAISDINKLPQSSAKKQLLQLTNYLLKRKI"
     gene            complement(3830..5578)
                     /gene="cydC"
                     /locus_tag="VBD27_000565"
     CDS             complement(3830..5578)
                     /gene="cydC"
                     /locus_tag="VBD27_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEO7024600.1"
                     /translation="MFKIPLFKELKTDQWIKPFFKQYKVSLVIALFLGFMTFFSASAL
                     MFNSGYLISKSASLPSNILLVYVPIVLTRAFGIGRPVFRYIERLTSHNWVLRMTSQLR
                     LKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIAWILYSFIIIA
                     TGFFSLWFALMMLLYLAIMIFLFPLWSILANGARQTREKELKNHLYTDLTDNVLGISD
                     WIFSQRGQEYVTLHERSESELMAVQKKIRSFDNRRALIVELVFGFLAILVIIWASNQF
                     IGHRGGEANWIAAFVLTVFPLSEAFAGLSAAAQETNKYSDSIHRLNELSETYFETTQN
                     QLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKSTLASLLRG
                     DLKASQGEITLGGADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVW
                     KVLERVGLKEMVTDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTV
                     GLDPITEQALLRVFMKELEGKTLVWITHHLKGIEHADRILFIENGQLELEGSPQELSQ
                     SSQRYRQLKAADDGDL"
     gene            complement(5571..7289)
                     /gene="cydD"
                     /locus_tag="VBD27_000566"
     CDS             complement(5571..7289)
                     /gene="cydD"
                     /locus_tag="VBD27_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEO7024601.1"
                     /translation="MLDKAVMRLSGIHKLLGLLAGLDVLQAIFIIGQAYYLSLSITGL
                     WEGQKLSSQTVYVLLFMVSYLGRHVIDYIKNRKLDDFSTAQSSLLRRQLLDKLFDLGP
                     KVVQEQGTGNVVTMALDGVSLVENYLRLVLNKMINMSIIPWIILAYIFYLDIESGAIL
                     LIVFPLIIIFMIILGYAAQAKADKQYESYQVLSNHFLDSLRGIDTLKYFGLSKRYGKS
                     IYQTSESFRKATMSTLKIGILSTFALDFFTTLSIAIVAVFLGLRLLNEQIYLLPALTI
                     LILSPEYFLPVRDFSSDYHATLDGKNAFQAIQKVLNKTGIKGEQLVIDDWSKESRLDL
                     ENIAIAYDQKRVVEDVTLRFRGHQKVALVGVSGSGKSSLINLLSGFLGPDNGSLKVDG
                     REVTNLDQEDWHKQMIYIPQTPYVFEMSLRDNITFYTPNASDEEVVRAIHMVGLDSLL
                     SELPDGLETRIGNGARPLSGGQAQRIALARAFLDQNRRIMVFDEPTAHLDIETELELK
                     EKMLPLMSDRLVIFATHRLHWLNQMDVIVVMEKGRVAEVGSYQELLAKKGYLYQLKHA
                     MGGNDV"
     gene            complement(7289..8308)
                     /gene="cydB"
                     /locus_tag="VBD27_000567"
     CDS             complement(7289..8308)
                     /gene="cydB"
                     /locus_tag="VBD27_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEO7024602.1"
                     /translation="MSALQFFWFFLIGLLFSGFFFLEGFDFGVGMAVQTLTHNEHEKD
                     QVVETIGPVWDGNEVWLLTGGGAMFASFPYWYASLFSGYYLILLTILFGLIIRGVSFE
                     FRHKVPAEKKQFWNWTLTIGSAIVPFFFGIMFISLIQGMPLDASGNLSAQFSDYFNIF
                     SLVGGVAMVLLAYLHGLNYIALKTEGPIRERARNYAQLLYWVLYLGLALFAVLLYFKT
                     DFFSNHPIVTTIMVLVIVVLAVLAHASTFKGAEMTAFLASGLSLVSVVVLLFQGLFPR
                     VMISSISPKYDLLIQNASSTPYTLKVMSIVAITLVPFVLAYTAWAYYIFRKRITLPVI
                     VTGEK"
     gene            complement(8309..9736)
                     /locus_tag="VBD27_000568"
     CDS             complement(8309..9736)
                     /locus_tag="VBD27_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEO7024603.1"
                     /translation="MTIETLARFQFAMTTVFHFFFVPFTIGTCLVVAIMETMYVITKN
                     EEYKKLTKFWGNIMLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAIEALLA
                     FFMESTFLGLWMFTWDNKKISKKLHVTFIWLVVFGSLMSAMWILTANSFMQHPVGYEV
                     VNGRAQMTDFLALVKNPQFFYEFTHVIFGAITMGGTVVAGMSAFRLLKSEQLKDTTVE
                     LYKKSVRIGLVVALLGSISVMGVGDLQMKALIHDQPMKFAAMEGDYEDSGDPAAWSVV
                     AWANEAEHKQVFGIKIPYMLSILSYGKPSGSVKGMDTANKELVAKYGKDNYYPMVNLL
                     FYGFRTMAAMGTAIMGVSVLGLFLTRKKKPILYKHKWMLWIVALTTFAPFLANTFGWI
                     VTEQGRYPWTVYGLFKIKDSVSPNVSVASLFVSNTVYFLLFGGLAVMMISLTIRELKK
                     GPEYEDEHGHHGAYTSIDPFEKGAY"
     gene            complement(9839..11047)
                     /locus_tag="VBD27_000569"
     CDS             complement(9839..11047)
                     /locus_tag="VBD27_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7024604.1"
                     /translation="MKEILVLGAGYAGLKAVRNLQKQSGDFHITLVDMNDYHYEATEL
                     HEVAAGSQPKEKITFPIKDVINTNKVNFMQDEVLRVDAENKTVTVKNNGELHYDYVVV
                     ALGFVSETFGIKGAMENALQMTNISQAENIHNHIVNTMKLYRETKDENLLKLLVCGAG
                     FTGIELAGAMVDERPKYAALAGVKPEQIEIICVEAATRILPMFDDELAQYGVNLIKDL
                     GINLMLGSMIKEIKPGEVVYGTSKEDEELKSITAGTIIWTTGVSGSPVMGESGFDQRR
                     GRVMVNSDLRDPKYDNVYVIGDVSAFMDTESGRPFPTTAQIATRMGAHVAKNLLHQIK
                     GEATEDFSYSPQGTVASVGNTHGLGVVGKTKIKKYPASVMKKIIMNKSLVDMGGLKEL
                     LAKGRFDLYH"
     gene            complement(11060..11959)
                     /locus_tag="VBD27_000570"
     CDS             complement(11060..11959)
                     /locus_tag="VBD27_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEO7024605.1"
                     /translation="MTLPIFLELVEMKAKTASVLPFLIGLCFSAYYYNSVHPVYVGLF
                     FVAMFLFNMFVDIWNNYNDYRNAVDLDYKNDTNIIGRENLSLRQIEVIMASLVITSSM
                     IGLVLVSQVGLPLLWMGLFCFGIGVLYSFGPRPLSSLPLGEVFSGLTMGFMISLICVY
                     LNTYQNFSWDILNLSKIFLISLPNTLWIANLMLANNLCDKEEDEKNHRYTLVHYTGIR
                     GGLLLFAISNSIALLAIVFEFLFGLAPVTVLLSLLLIPFIYKQTKLLWQKQVKRETFV
                     CAVRILALGSATQVLTYLIGIIF"
     gene            complement(12368..12973)
                     /locus_tag="VBD27_000571"
     CDS             complement(12368..12973)
                     /locus_tag="VBD27_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024606.1"
                     /translation="MNKLITEKKVNNVSTANYLKLGLVSAMFAGGAFVALGSTQGVSA
                     STFTAPQATPPKAERQLTDSELYERAQKQVLPRYIQGSLSGILNQHSTLYKQQNAAVT
                     PQVSSPKSERQLTDSELYESAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVTPQVSS
                     PKSERQLTDSEIYERAQKQVLPNYIQGSLSGILNQHSTLNA"
     gene            13856..14155
                     /locus_tag="VBD27_000572"
     CDS             13856..14155
                     /locus_tag="VBD27_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO7024607.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQTQADLAASQFTGKSAQELVTVTFT
                     GDKKLISIDYKEAVVDPEDIETLQDMTTQAINDALSQVDDATKKIMGAFAGKMPF"
     gene            14315..15505
                     /locus_tag="VBD27_000573"
     CDS             14315..15505
                     /locus_tag="VBD27_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7024608.1"
                     /translation="MVMDSLIIKQLIKSTFDIPLQVTYPNGNIETYNGSNPHVKLKLN
                     KNFSVSELSKDPSIVLGEAVMDGDIEIYGSIQELILSAYRCGDSFLRNSKFSKLIPKQ
                     FHDKKHSKSDIQKHYDIGNDFYKLWLDDTMTYSCAYFKHENDSLEQAQLNKVHHILNK
                     LNAQPGGKLLDIGCGWGTLIITAAKEYGLNATGITLSEEQASFITKRIKEEGLENKVT
                     VLIKDYRDIRETYDYITSVGMFEHVGKENLSQYFQTISKRLNINGLALIHGITGQVGG
                     NHGSGTNSWINKYIFPGGYIPRLTENLNHIASAGLQIADLEPLRRHYQKTLELWTKNF
                     HNALPEVQKTHDKRFINMWDLYLQSCAASFESGNIDIFQYLLSKGVSKDTMPMTRDYM
                     YSAN"
     gene            complement(15575..16300)
                     /locus_tag="VBD27_000574"
                     /pseudo
     CDS             complement(15575..16300)
                     /locus_tag="VBD27_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(16427..17014)
                     /locus_tag="VBD27_000575"
     CDS             complement(16427..17014)
                     /locus_tag="VBD27_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEO7024609.1"
                     /translation="MKQLQEYIAFDLEFNTVGEHSHIIQVSAVKYSNHQEIALFDTYV
                     HTKVPLQSFINGLTGITARDIIGAPKIEIVLTDFQSFVGDTPLIGYNGYKSDLPLLVE
                     NGLDLTSQYQVDLYDEAFVRRSTDLNGIVNLKLTTVADFLGIKGKAHNSLEDARMTAR
                     VYEKFLDLDENKIYLKQQKEVAVDSPFATLGNLFD"
     gene            complement(17058..17594)
                     /locus_tag="VBD27_000576"
     CDS             complement(17058..17594)
                     /locus_tag="VBD27_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO7024610.1"
                     /translation="MTVEKTVSELAEILGVSRQAMNNRVKTLPEECVEKNSKGVTVVN
                     RDGLIKLEEIYKKTILEEEPIDEEASRRELLEILVDEKNTEITRLYEQLKAKDIQIAS
                     KDEQLHVKDIQIAEKDKQLDQQQQLTLTAMEDTQRLQLELNEAKAEVEEIQEAKEEKI
                     QELEAVKKSFFGRFFNKK"
     gene            17729..18901
                     /locus_tag="VBD27_000577"
     CDS             17729..18901
                     /locus_tag="VBD27_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7024611.1"
                     /translation="MKHYDTIIIGGGPAGMMAAISSNFYGNKTLLIEKNKRLGKKLAG
                     TGGGRCNVTNNGNLDELLAGIPGNGRFLYSVFSQFDNHDIINFFQDNGVTLKEEDHGR
                     MFPTTDKSRTIINALENKIKELGGQIMTDTEVVSVKKIGDSFYIKTKDTQFASDKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLEVTDMEAAESPLLTDFPHKKLQGISLDDVTLSF
                     EKHIITHDLLFTHFGLSGPAALRISSFVKGGETIYLDVLPNISVKELEIHFQNEREKS
                     LKNALKILLPERLAEFYAEDLPEKVKQVSVKDLEMLIQKLKKLPILVTGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVAGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHY"
     gene            complement(18945..19727)
                     /locus_tag="VBD27_000578"
     CDS             complement(18945..19727)
                     /locus_tag="VBD27_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEO7024612.1"
                     /translation="MCYNNSMNERYIRFNPNIAKQKPSNKDKCPFCDKSQLGKILDVK
                     DDMIWVENKYPTLEETYQTLVIESNDHNGDISVYSESKMRQLLDYLLSKWQLMEESGH
                     YRSVVLYRNFGPLSGGSLRHPHSQIVGLKNVDAYETLNLDWFDGIEVCSETSQQAEIS
                     IANRPMLDFTEFTIKVSKLENAILIADSLPILCHYILNEFLGGRCKSYNLFFYKHDGG
                     LICKIVPRFIVSPYQIGYAIHQVNLPDELEKIKEALRAKYYL"
     gene            19886..20155
                     /gene="rpsN"
                     /locus_tag="VBD27_000579"
     CDS             19886..20155
                     /gene="rpsN"
                     /locus_tag="VBD27_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO7024613.1"
                     /translation="MAKKSKIAKFQKQQKLVEQYAELRRELKEKGDYEALRKLPKDSN
                     PNRLKNRDLIDGRPHAYMRKFGMSRINFRNLAYKGQIPGIKKASW"
BASE COUNT         6871 a         3908 c         3195 g         6294 t
ORIGIN      
        1 tcattattac aaatgttgag aaattaaaag atcatattgt attttaatat aacaatagaa
       61 aggatatatt atgcgttata atcaattcag ctacattcca actaaaccta acgaagcttt
      121 tgaagagctc aaaggactag gttttccatt aaacaaaaag aattctgata aagctaattt
      181 ggaagctttt ctcagacatt cttttttaaa tcaaactgat actgattacg ctcttagtct
      241 ccttatcgtt gatgcaaaaa ccgatgctct aacctttttt aaatcaaata gtgacttaac
      301 actagaaaat ttacaatgga tttatttaca gttattaggc tttatccctt ttgtagactt
      361 taaagaccct aaagcattct tacaagatat taacttccca gtctcatatg acaatatttt
      421 tcaaagtcta catcacttac tcgcctgtcg tggaaaatct ggcaatacat taatcgacca
      481 attagttgct gatggtttac ttcatgcaga taatcactac cattttttca atgggaagtc
      541 tctggccact ttcaatacta accaattgat tcgcgaagtt gtctatgttg aaacatcctt
      601 agatactatg tctagtggtg aacatgattt agtaaaagtt aacattatca gacccactac
      661 cgagcatact atccctacga tgatgacagc tagcccctat catcaaggta tcaatgatcc
      721 tgccgcagac caaaaaacat accaaatgga gggtgcgcta gcagttaaac aacctaaaca
      781 catacaagtt gacacaaaac catttaaaga agaagtaaaa catccttcaa aattacccat
      841 cagccctgca actgaaagct tcacacacat tgacagttat agtctcaatg actattttct
      901 ttctcggggt tttgctaata tatacgtttc aggtgtgggt actgctggct ctacgggttt
      961 catgaccagt ggggattacc aacaaataca aagctttaaa gcagtcattg attggttaaa
     1021 tggtaaggtt actgcattca caagtcataa acgagataaa caagtcaagg ctaattggtc
     1081 aaatggcctt gtagcaacca caggtaaatc ttatcttggt accatgtcaa ctggtttagc
     1141 aacaactggc gttgaggggc tgaaagtcat tatcgctgaa gccgcaatct ccacatggta
     1201 tgattattat cgagaaaatg ggcttgtgtg tagtccaggc ggctaccccg gtgaagattt
     1261 agacgtttta acagaattaa catactcacg aaacctctta gctggtgatt acatcaaaaa
     1321 caacgattgc tatcaagcat tgttaaatga acaatcaaaa gcaattgacc gtcaaagtgg
     1381 ggattacaac caatactggc atgaccgtaa ttacctaact cacgtcaata atgtcaaaag
     1441 tcgagtagtt tacactcatg gactacagga ttggaatgtt aagccaagac atgtctataa
     1501 agttttcaat gcattgcctc aaaccatcaa aaaacacctt tttttacatc aaggtcaaca
     1561 tgtgtatatg cataattggc agtcgattga ttttcgtgaa agcatgaatg ccttactaag
     1621 ccaagaacta cttggcattg acaatcattt ccaattagaa gaggtcattt ggcaagataa
     1681 tactactgag caaacttggc aagttttaga tgctttcgga ggaaaccatc aagagcaaat
     1741 tggtttaggt gatagtaaaa aacttattga taaccattat gacaaagaag cctttgatac
     1801 ttattgtaaa gacttcaatg tgttcaaaaa tgatcttttc aagggaaata ataaaaccaa
     1861 tcaaatcact attaatcttc ctctaaagaa aaattatctc ctgaatggac agtgcaaact
     1921 ccatctacgt gttaaaacta gtgacaaaaa ggccatttta tcagcccaaa tcttagacta
     1981 tggtcctaaa aaacgattca aagatacacc aaccatcaca ttcttaaaca gccttgataa
     2041 tggtaaaaat tttgccagag aagctttacg tgaactcccg tttactaaag atcattatcg
     2101 tgtcatcagt aaaggtgtct tgaaccttca aaatcgtaca gacttactta caattgaggc
     2161 tatcgagcca gaacaatggt ttgatatcga gtttagcctc caaccaagta tatatcaatt
     2221 gagtaaaggt gataatctaa ggattatcct ttatacaact gattttgaac ataccattcg
     2281 agataatgct agttattcta taacagtaga tttaagtcaa tcttatttaa ctatcccaac
     2341 taatcaagga aattaactta tgaaacttct cactaaagaa cggtttgatg attctcaaca
     2401 cttttggtac cagatcaatt tattacaaga gagtaacttc ggagcagttt ttgaccatga
     2461 taataaaaac attccacagg ttgttgcaac tattgttgat gatttacaag gttccggaag
     2521 ttcgaattat ttctggtatt ttggcaatac tactgatact tccatcctta tgattgctca
     2581 tttaaatcga aaattctata ttcaggttaa tttaaagaac tttgactttg cactcaattt
     2641 aatagctata aataattgga agagtctcct ccaaactcaa ctcgaagctc taaacgatac
     2701 cctagcaata tttcaataaa taaggtagaa tggagtgaca aagcaacgcg agggagactg
     2761 attaatgtca tcttattgga ataactatcc tgaacttaaa aaaaatattg atgaaaccaa
     2821 tcaactaatt caagaaagaa tacaggtcag aaataaagat attgaagcgg cgctaagcca
     2881 actcacagct gcgggaggaa aacagctcag accagcattc ttttaccttt tttctcaact
     2941 tggtaataag gagaatcaag atactcagca actaaagaaa atcgctgctt ctttagaaat
     3001 ccttcacgtt gctacattaa tccatgatga tgtcattgat gactcaccac taagacgtgg
     3061 aaatatgacc attcaaagca agtttggcaa agacatcgca gtttacactg gggatttact
     3121 tttcacagtc tttttcgatc ttattttaga atctatggct gatacaccat ttatgaggat
     3181 taatgcaaaa tctatgcgta aaattctcat gggagaattg gaccagatgc accttcgtta
     3241 caatcaacaa caaggtatcc atcactattt acgtgcgatt tcaggtaaga cagccgaact
     3301 ctttaaatta gctagcaaag aaggagctta ctttggtggt gcagagaagg aggttgttcg
     3361 tctagcaggc catatcggat ttaacattgg tatgacattc caaattttgg atgatatcct
     3421 ggattatact gcagataaaa aaacatttaa caagcctgtc ttagaggatt tagcacaagg
     3481 catttacagc cttcctctac ttcttgccat tgaagaaaat cctgatattt tcaaacctat
     3541 tttagataaa aaaacagata tggctactga agacatggaa aaaattgctt atctcgtcgt
     3601 ttcccataga ggtgttgaca aagctcgcca tctagctcgt aaatttactg aaaaagctat
     3661 tagtgacata aataagctac cccagagctc tgcaaaaaaa cagttgctac aattaactaa
     3721 ttacctttta aaacgcaaaa tttaaataat aaaaaaacat tccacaatgc tagaaaagca
     3781 gttagggaat gtttttttat tatcatttat ttatcgcacc tatcaatcat catagatcac
     3841 catcatcagc ggctttcagc tgacggtaac gttggctact ttgagacaat tcttgaggag
     3901 aaccttccaa ctctaattgc ccattttcta taaataagat acgatcagca tgttcaatac
     3961 cttttaagtg atgtgtaatc caaactaagg tcttaccttc caattctttc ataaataccc
     4021 ttagtaaggc ttgttcagta ataggatcaa gtccaacagt tggctcatct aagataacaa
     4081 ttgggacatc ttttagtaag attctagcca aagcaattct atgcctttcg ccacctgaaa
     4141 acctaagtcc agcttcatca accattgtat agagaccatc tgataaatca gtgaccatct
     4201 ctttcaatcc aactcgttca agaactttcc atacatcttc ttcactagca tcttggtttc
     4261 caatgcgaat gttatttagc agggttgtat taaaaaggta gggcgcttgt tgtatcactc
     4321 caatatagtt agaaatgcaa tcaccaacta ttgaaacatc agcaccgcct agggtaatct
     4381 ccccttgact tgctttcaag tcgccacgaa gtagactggc taaggtactc ttgccagaac
     4441 cactacgccc taaaatagca attttttctc cttctttaat atccaaatct aaatgatgca
     4501 aaacccattt ctcttgtggc ttatactgga aacttaaatt cttgacggaa aaatcgtatg
     4561 gcttattagg caactgattt tgagttgttt caaaatacgt ttctgataat tcattaagac
     4621 gatgaattga atcagaatat ttatttgttt cttgagcagc agcagacaaa ccagcaaatg
     4681 cctctgacaa gggaaatact gttagaacaa atgctgcaat ccagttagct tcgccacctc
     4741 tgtgaccaat aaactgatta ctagcccaaa taataaccaa aatagctaga aagccaaaaa
     4801 ctaattccac aataagagct ctacgattat caaaggaacg aatttttttc tgaacagcca
     4861 tgagttcact ttccgaacgc tcatgcaagg tgacgtactc ttgaccacgt tgactaaaaa
     4921 tccagtctga aatacctaag acattatccg tcaaatcagt ataaagatga ttttttaatt
     4981 ctttttcacg agtctgacga gcaccattag ctaaaatcga ccaaagtgga aaaagaaaaa
     5041 tcataatagc taaataaagc aacatcatta aagcaaacca tagagaaaag aagcctgttg
     5101 caataataat aaagctatat aatatccacg ctataatcgt tggaaatatt gtcctaagat
     5161 acaaattttg caaataatta atatcctccg ctaaaagccc cataacatct cctaaacgaa
     5221 aatcacgctt cataaagata gcattgcttt ctaaggaatg gtaaagcttc aagcgcagct
     5281 gtgaagtcat tcgtaatacc caattgtgac tcgttaggcg ttctatataa cgaaaaacag
     5341 gacgtccaat gccaaaagca cgtgttagta caattggaac atatactaga agaatattac
     5401 tcggtagtga agctgactta ctaatcagat agcccgaatt aaacataaga gcactggcag
     5461 aaaagaaagt cataaaacca aggaaaagag caattactaa ggaaacttta tattgtttga
     5521 aaaatggttt tatccattga tctgtcttta attctttaaa caaagggatt ttaaacatca
     5581 tttcctccca tagcatgctt cagttgatag agatatcctt ttttagctaa taattcctga
     5641 taagagccta cttctgcaac tctccccttc tccataacga ctataacatc catttggttc
     5701 aaccagtgaa gacgatgagt tgcaaagata actaaacgat ctgacatcag tggcaacatt
     5761 ttctctttta attctaactc tgtttcaata tctaaatgag cagttggttc atcaaaaacc
     5821 ataatacgac gattttgatc aagaaaagca cgagccagag ctatacgctg ggcttgtcct
     5881 ccactaagcg gacgcgctcc attaccaata cgcgtttcta aaccgtcagg taactcgctt
     5941 agcaagctat ctaaacctac catatgaatc gctcttacca cctcttcgtc actagcattg
     6001 ggagtataga aggtaatatt atcacgtaat gacatttcaa aaacataagg agtttgagga
     6061 atatagatca tctgtttatg ccaatcttct tgatcaaggt tagtcacttc tctaccatcc
     6121 acttttaaag aaccattatc aggccctaaa aaaccactca gaaggttaat taagctagac
     6181 ttacctgaac cactaactcc tactaaagca accttttgat gtcctctaaa tcgtaaggtt
     6241 acatcctcaa caacacgttt ttggtcgtac gcaatagcaa tgttttctaa atccaaacga
     6301 ctctctttag accaatcatc tatgactaat tgctcacctt taataccagt tttgtttaga
     6361 actttttgaa tcgcttgaaa tgcatttttc ccgtctaacg ttgcatgata atcactagaa
     6421 aaatctctta cgggtaaaaa gtattcaggg gataaaatca aaattgttaa agccggtaaa
     6481 aggtaaatct gctcatttaa taatcgtaaa cctagaaaaa cagcaacgat agcaatggat
     6541 aaagttgtaa aaaagtctaa agcaaacgtt gataaaatac caattttcaa agtactcatt
     6601 gttgctttac gaaaagattc actagtttga taaatgcttt taccataacg tttactaaga
     6661 ccaaaatatt tgagagtatc aatgcctcgt aatgaatcta aaaaatgatt agaaagtact
     6721 tgataggatt catattgttt atctgcttta gcttgagccg catagcctaa aataatcata
     6781 aagatgataa ttaaaggaaa aacgataagt aaaattgcac ctgattcaat atctaaatag
     6841 aaaatataag ctagaataat ccatgggata atagacatgt taatcatctt attcaagact
     6901 aaacgtaaat aattttcaac taaagaaacg ccatctaatg ccattgtaac aacattacca
     6961 gttccttgtt cttgaacgac tttaggacct agatcaaata acttatctaa taattgacgt
     7021 ctcaaaagac tagattgtgc tgttgaaaag tcatcaagtt ttctattttt gatgtaatca
     7081 ataacatgac ggcctaaata tgagaccata aataacaaaa cataaaccgt ttgagaagaa
     7141 agtttttgcc cttcccacaa accagttata gaaagactca aataataagc ttgtccgata
     7201 ataaagattg cttgaagaac atcaagtccc gcaagcaatc ctaacaactt atgaatacct
     7261 gacaagcgca taaccgcttt atctagcatt atttttctcc tgtcacaatc actggcaagg
     7321 taatacgttt tctaaagata taatatgccc atgctgtata agctagaaca aagggaacta
     7381 gagtaattgc gacaatcgac attactttta aagtatatgg cgttgaggat gcattttgaa
     7441 tcagcaaatc atatttaggg ctaattgatg aaatcataac tcgtgggaat agaccttgga
     7501 acaacagaac aaccactgaa acaagactga gaccactcgc taaaaatgct gtcatctctg
     7561 ctcctttaaa agttgaagca tgcgctaata cagctaatac aacaataacc aaaaccataa
     7621 ttgtcgtaac tatcggatga ttgctaaaga aatctgtctt gaaatacaag agaactgcaa
     7681 acaaggctaa accaagatag agtacccaat aaagcaactg agcataatta cgggcacgct
     7741 ctctaattgg cccttctgtc ttcaatgcaa tgtaatttaa accatgaaga taggctagca
     7801 ataccattgc aactccaccc accaacgaga atatattaaa gtaatcactg aattgagctg
     7861 ataagttacc actggcatca agtggcatac cttgaatcaa acttataaac ataatcccaa
     7921 agaagaatgg aacaatagcg gaaccaattg ttaaggtcca gttccaaaac tgctttttct
     7981 ctgcaggaac tttatgtcta aattcgaagg aaacaccacg aataattaat ccaaagagaa
     8041 tcgtaaggag aatcaaatag taaccactaa atagtgaagc ataccagtat ggaaaagaag
     8101 caaacattgc tccaccaccg gtaagaagcc aaacttcatt accatcccaa actgggccaa
     8161 tggtttctac tacttgatct ttttcatgct cattatgtgt taaagtttga acagccatcc
     8221 caaccccaaa gtcgaatcct tcaaggaaga agaatcctga aaataggagg ccgattagga
     8281 aaaaccaaaa gaattgtaaa gcactcattt agtatgctcc tttctcaaag ggatcaattg
     8341 aagtatatgc accatgatgt ccgtgttcat cttcatactc tggccctttc ttcaattctc
     8401 ttattgttag gcttatcatc atcaccgcta aaccaccaaa caacaagaaa tacacagtat
     8461 tggaaacaaa taatgatgca actgaaacgt ttggagagac agagtcctta atcttgaaaa
     8521 gaccatagac agtccaaggg tatcgtcctt gttctgtcac aatccaacca aatgtattgg
     8581 ctaagaatgg tgcaaaggtt gttaaagcaa caatccaaag catccatttg tgtttataaa
     8641 gaattggttt tttcttacga gttaaaaaga gtcctaaaac agaaacaccc ataatagctg
     8701 ttcccatcgc cgccattgta cggaaaccat agaaaagtag gttaaccatt ggatagtagt
     8761 tatctttacc atacttagca accaattctt tattagctgt atccatacct ttcacagaac
     8821 cagatggttt cccgtaagat aaaatactca acatataagg aatcttaatc ccaaaaactt
     8881 gtttgtgttc tgcttcattt gcccaagcaa ctacactcca agctgcagga tcacccgaat
     8941 cttcataatc accttccata gcagcaaatt tcattggttg atcgtgaatc aaggctttca
     9001 tttgtaaatc cccaactccc ataactgaaa ttgaaccaag aagtgctact actaaaccaa
     9061 tacgaaccga tttcttataa agttcaaccg ttgtatcttt cagctgttct gattttaaga
     9121 ggcggaaggc tgacatacca gcaaccactg taccacccat cgtaatggca ccaaaaataa
     9181 catgggtaaa ttcatagaag aactgtggat tttttacaag tgcaagaaaa tcagtcattt
     9241 gcgctctacc gtttacaact tcataaccaa caggatgttg catgaagcta ttagcagtaa
     9301 gaatccacat cgctgacatt aatgagccaa aaaccactag ccaaatgaat gtcacatgta
     9361 attttttact aatttttttg ttatcccatg taaacatcca taaaccaagg aaagtagatt
     9421 ccataaagaa agctaataaa gcttcaatag ccaatggagc tccaaaaata tcacctacga
     9481 aacgtgaata gtctgaccag ttcattccaa attggaactc ttgaatgata cctgtcacaa
     9541 caccaaccgc aaaactaagg agcatgatat taccccaaaa ttttgtcaat tttttatact
     9601 cttcattttt tgtgataaca tacattgtct ccataatagc aaccactagg catgtaccaa
     9661 ttgtaaatgg tacaaagaaa aagtggaaaa ctgtagtcat cgcaaattga aaacgagcta
     9721 aagtttcaat tgtcatacaa aacctcttat tttgttttaa tcatttcttt tatagaaata
     9781 tctgcaagag ctagtttcct agcttcgtaa caattggtaa ataacttact ttaatatctt
     9841 aatgatataa atcaaaacgt cccttagcta gaagttcttt aagtccaccc atatcaacaa
     9901 gtgatttgtt catgataatc ttcttcataa ccgacgcagg gtatttttta attttagttt
     9961 ttccaacaac accaagtcca tgcgtatttc ctactgaagc aactgtgcct tgcggactat
    10021 acgaaaagtc ttctgtagct tcacctttga tttgatgtaa aaggttttta gcaacatgag
    10081 ctcccatacg agttgcaatt tgagctgttg ttgggaatgg gcgaccagac tcagtgtcca
    10141 taaatgcaga cacatcaccg ataacataaa cattatcgta ttttggatca cgcaaatcac
    10201 tgttgaccat tacacgtcca cgacgttgat caaatcctga ttctcccata accggacttc
    10261 cactaacacc tgttgtccaa atgatagtac ctgcggtaat agatttgagt tcttcatcct
    10321 ctttactagt accataaaca acttcacccg gtttgatttc cttaatcata cttccaagca
    10381 tcaaattaat acctaaatcc ttaattaagt tgacaccata ttgcgctaat tcatcatcaa
    10441 acattggtag gatgcgagtt gctgcctcta cacaaataat ttcaatttgt tcaggtttta
    10501 cacccgctaa agcagcatat tttggtcgct catcaaccat agctccggct aactcaatac
    10561 ctgtaaaacc agcaccacat actaagagtt tgagaagatt ttcatctttt gtctcacggt
    10621 acaacttcat tgtattaaca atgtgattat ggatgttttc tgcttgcgaa atattggtca
    10681 tctgcaaagc attctccata gcacccttga tgccaaaagt ttcagataca aagcctaagg
    10741 caactacgac ataatcataa tggagttcac cgttattttt aactgttact gttttatttt
    10801 cagcatcgac tctaagcacc tcatcttgca tgaagttaac cttattagtg ttgataacat
    10861 ccttaattgg gaatgtaatt ttttcttttg gctgagagcc tgcagctact tcatgtaatt
    10921 ctgtagcttc gtagtgataa tcattcatgt caacaagggt aatatgaaaa tcacctgatt
    10981 gtttttgtaa attacgaact gctttcaaac cggcataacc agcacctaaa actaggattt
    11041 ctttcatgat aaaactcctc taaaaaatga ttccaattag atacgttaag acttgggttg
    11101 cagatcccag tgctagaata cgtaccgcac aaacaaatgt ttctctctta acctgttttt
    11161 gccacaatag ttttgtctgc ttataaataa atggtattaa taacaaactc aagagtactg
    11221 taacaggcgc aagtccaaaa agaaattcaa aaacaattgc caataaagct atactattag
    11281 aaatagcaaa cagcaagagt ccacctctta tccctgtata atgtacaagg gtatagcgat
    11341 gatttttttc atcttcttct ttgtcgcaca gattattcgc caacattagg ttggcaatcc
    11401 ataaggtgtt cggcaaggaa atcaagaaaa ttttgcttaa gttgaggata tcccacgaaa
    11461 agttttgata agtgtttaga tatacacaaa ttaaactaat cataaatccc attgtcaagc
    11521 cagaaaatac ttctcctaag ggcagactag ataaaggtct tggaccaaag gaatataaaa
    11581 caccaattcc aaaacaaaaa agtcccatcc ataaaagagg cagaccgact tgggaaacta
    11641 aaactaaacc aatcatacta gaagtaatga ctaaacttgc cataataact tctatttggc
    11701 gtagggatag gttctcacga ccaataatgt tagtgtcatt cttataatcc aaatccacag
    11761 cattccgata atcattgtaa ttattccaaa tgtcaacgaa catattgaat aaaaacatag
    11821 caacgaagaa taaaccgaca tatacagggt gtactgaatt gtagtaataa gcactaaaac
    11881 acaaaccaat caaaaatggt aaaacactag ctgttttggc tttcatttct accaactcta
    11941 gaaagattgg taatgtcatt attaaagtcc tctctataca taatcttcaa caatactatt
    12001 ataatgcaat ttctaccatt tttaaaacaa tagggcttga tttttgatta aaaatacaaa
    12061 aaatttttct taaaatgagt taatcacact tagaacacta aatactattt tgaggagtcc
    12121 tattcatcac actatgccat gtaatattcg gaaaaatagt attgcaaccc tcaactatcc
    12181 cttatgttgg caccagcctt tgaaagtcct ctgattgcac tgcttagtta gtattttcat
    12241 cagctgtatg atctctaaga gccatcagca tactaaaaaa actagtatta cactttgaaa
    12301 agtgtaacac tagctccaat ttgttttaaa tagcttaact atttaatcat taatctactt
    12361 tatatattta agcatttaaa gttgaatgtt ggttgaggat acctgagaga gaaccttgaa
    12421 tataatttgg caatacttgt ttttgagcac gttcatagat ttctgaatct gttaactgac
    12481 gttctgattt ggggcttgat acttgcggtg tcactgctgc attttgttgt ttgtaaagtg
    12541 ttgaatgctg gttaaggata cctgagagag aaccttggat atattttggt aatacttgtt
    12601 tttgggcact ttcataaagt tctgaatctg ttaactgacg ttctgatttg gggcttgata
    12661 cttgcggtgt cactgctgca ttttgttgtt tgtaaagtgt tgaatgctgg ttaaggatac
    12721 ctgagagaga accttggata tatcttggca atacttgttt ttgggcacgt tcataaagtt
    12781 ctgaatctgt taactgacgt tctgcttttg gaggtgttgc ttgcggtgct gtgaaagttg
    12841 aagctgaaac accttgtgta gaaccaagag ctacgaatgc tcctccagca aacattgctg
    12901 agacaagacc aagtttcaaa tagttagcag ttgagacgtt gttgactttt ttttctgtaa
    12961 taagtttgtt cataagaatt acctctttct tttgggttat ttattttatg aactaagtat
    13021 aacaacaaga aataaagtaa ggttaaacta aatataaaat aattcttaag tcattcttag
    13081 gaaaccataa aatcatacac taacaaaaaa tttggtatct agctcaaaag taaaagtaga
    13141 ataaaggctt ttacttctga gctagtaatg aataaattat atttgcatct agtttggaat
    13201 tagccctagt gtattataat gatccctaca aatatgtgcc aaacaccttc ttcatatata
    13261 caagattgat ttaacattcc tataccctta atggtgggca cattctcttc atgttccata
    13321 tagaaatcaa cagataattc cgtattgtcc ctattattaa attttccagt taatgataaa
    13381 ttatatcgtc caccttgagg tgtcctatta ccaatctgtt caacctttac acttgacgaa
    13441 tcgaattgaa cagattctgt cttatcattc tgtgatttag aacatcaaca atttcttgtt
    13501 catgttcttt gaggtaagct agttgttctt ctttaacgtg cttttatctt tcgtacttac
    13561 tgatacttga gagcattttt ttgaccctac ttcttactaa tcagctgacg tccctataag
    13621 atgaatcata aacacatagc tgccactaac cattttaacc ttttatgcat acataattct
    13681 tcttgatatc ccataccatt atctttagta taaacactta ataaaacaag catttaacaa
    13741 ctaaggttaa taaaattcaa taaatctaat ccttctactt actttagttt atcacttttt
    13801 tctctcatcc tatgctataa tagtaaggta tcaaaattta ggagaatata gtcttatgat
    13861 gaatatgcaa aatatgatgc gtcaagcaca aaagcttcaa aaacaaatgg aacaaacgca
    13921 agctgattta gcagctagcc agtttactgg aaaatctgct caagagctcg ttaccgtgac
    13981 atttacaggc gataaaaagc taatttcaat tgattacaaa gaagctgtgg tagaccctga
    14041 agatattgaa acactacaag acatgacaac tcaagctatc aatgacgctt taagccaagt
    14101 tgacgatgct acaaaaaaaa ttatgggagc atttgcgggt aaaatgccat tctaaaaaat
    14161 aaatcacatt tactgaatca cctgtaaatg tgatttttat ttcaaacttc tttcattttt
    14221 tcacgaaaac aagttttatt ttcaaaaaat atttgcaaaa aatcagcaga gaggtataat
    14281 aacattgaaa atattagaga gaaggtgact aaccatggtt atggatagtc taatcataaa
    14341 acagttgatt aagagtacat ttgatatacc cttacaagtc acttatccaa atgggaatat
    14401 tgaaacttat aatggtagca atccccacgt caaacttaaa ctaaacaaaa atttctccgt
    14461 ctcggagcta tcaaaagacc cttccatagt attgggtgaa gctgttatgg atggagacat
    14521 tgaaatttat ggttctatac aagaattaat tttatcagct taccgctgtg gagacagctt
    14581 tctacgtaat agtaaattta gcaaattgat tcctaaacaa tttcatgata aaaaacacag
    14641 taaatccgac attcaaaagc attatgacat cggaaatgat ttctataaac tgtggcttga
    14701 tgataccatg acttattcat gcgcctattt taaacatgaa aatgatagtc tagagcaagc
    14761 tcaactaaac aaagtacacc atattcttaa taaattaaat gctcagccgg gagggaaatt
    14821 actagacatc ggttgtggct gggggacact tatcattact gctgctaaag aatatggctt
    14881 aaatgcgaca ggtatcacct taagtgaaga gcaggcttct ttcattacta agcgtatcaa
    14941 ggaagaaggg ttggaaaata aagtaacagt cctcattaaa gattatcgtg acattcgtga
    15001 aacatacgat tatatcacta gtgttggtat gtttgaacat gtaggaaaag aaaatttatc
    15061 tcaatacttc caaaccattt ctaaaagact caacatcaat ggtctagctc ttattcatgg
    15121 tattactgga caagtgggtg gtaatcatgg tagtggaact aattcttgga ttaataagta
    15181 tatcttccca ggaggatata tccctcgttt aactgaaaat ttaaatcata tagctagtgc
    15241 tggacttcaa attgctgact tagaaccgct tcgtcgtcac tatcaaaaga ctctcgaatt
    15301 atggacaaaa aatttccaca atgcccttcc tgaagttcag aaaactcatg ataagcgttt
    15361 tatcaatatg tgggacctct atcttcaatc ttgtgcggcc tcttttgaat ctggcaatat
    15421 tgatattttt caatacttgc tttcaaaagg tgtttcaaaa gacactatgc ctatgacacg
    15481 cgattatatg tattctgcta attgatatag ttaatattat agtattccaa cttattctaa
    15541 ataaaaagga ctctcttgaa ttcaagggag ttctttaacg ttcttctctg gctaactcta
    15601 tacaatgtga cttttggtga caatgtggac agaatacttt tcgcgtctta ggggtatgtt
    15661 tcgctagatt aaatgcttga tatgtcggtg taaaggtttg atgacaattt ggacaaagat
    15721 aaacaacctg ttgtttaaaa taccaaacca taccattcat agctattaag aatactgtta
    15781 ctgctatcca cataagccat ctttgattta gagaaactgc tgctattatt aacgtaatat
    15841 agaccataat aatcataacg acttgtagca ctatgctttt ttgtagtata ccccattttc
    15901 ttttgttttc ttccataagc acaatgtcag aaagatagcc taatgtttta ctatcttgtt
    15961 tttctacagt ttttagtagg ttgacagctt tgtcaagttt gtctttttta ctatcaatag
    16021 ctagccttaa ttctcttatc tacacttgta aaaaaagctc taaaatctgg ctagcattgt
    16081 cttcagtaaa tagatttttg atattatcaa ttgaaaaatc taaatctcgt aaataaataa
    16141 tttgtctaag cttttctaaa tcagcaatac tataaaccct cctacctcct tcagatagtt
    16201 ctgatgggga taaaattcct cttttgtcat agtactgaac agtacgaaca gtaactcctg
    16261 cttctttagc taagtcacca gtggaatatt tttctgacat gtttcacacc tcctttaata
    16321 tagtaaacac ttctttatca gaaacacctt tactatatca aatgacctaa ggtaataagc
    16381 aagtaataac gctaagtatt tttaacttta aataaaacta caacaattaa tcaaacaaat
    16441 ttcctaaagt cgcaaatggg ctatcaactg ctacctcttt ttgctgtttt aaatagattt
    16501 tattttcatc caagtccaga aatttttcat aaacacgcgc agtcatacgg gcatcctcta
    16561 agctattatg tgctttaccc tttattccta aaaagtcagc aacagtagtc aatttaaggt
    16621 tgacaatacc atttaaatct gtactgcgtc tcacaaaagc ttcatcatat aagtccacct
    16681 gatattgact agttagatcc aaaccatttt caactaaaag gggtaaatcc gatttatacc
    16741 cattataacc aatcagtggt gtatctccaa caaaactctg aaagtctgtc aaaactatct
    16801 caattttagg agcccctata atatctctag ctgtaatacc tgttaaacca ttaataaagc
    16861 tttgtaatgg aacttttgtg tgaacataag tatcaaataa agcaatctct tgatgattag
    16921 aatatttaac ggccgacact tgaataatat ggctatgctc accaaccgtg ttaaactcta
    16981 aatcaaaagc tatatattct tgtaattgtt tcattatttc tcctaaaaag cgcaaggaaa
    17041 ctccttacgc ttttattcta ttttttattg aagaaacgtc caaagaaact ttttttaaca
    17101 gcttctaatt cttgaatttt ttcttctttg gcttcttgaa tctcctcaac ctctgcttta
    17161 gcttcattta actcaagttg cagacgttgc gtatcttcca tagcagttaa agtcaattgc
    17221 tgttgttgat ctaattgctt atctttctct gctatttgaa tatcttttac atgtaactgc
    17281 tcgtcttttg aagcaatctg aatatctttt gcttttaact gttcatataa acgtgtgatt
    17341 tcagtattct tctcatcaac gagaatttct aaaagttctc gtcttgatgc ttcttcgtca
    17401 attggttcct cttccaaaat agttttttta tagatttctt ccaatttaat caagccatca
    17461 cgattaacaa ctgtaacgcc tttactattc ttttccacac attcttcagg gagtgtttta
    17521 actctgttat tcatcgcttg acgactaacc cctaaaattt cagctaattc actaaccgtc
    17581 ttttcaactg tcataacttc ctcttttgta ctgattttct tataaaagaa tatcacaatc
    17641 ctaatctagt gtcaatttac tgtcaactta aagtgtcaaa caagacattt tgacgtctat
    17701 ttctctattt atatgctaga ataagattat gaagcattat gacacaataa ttataggtgg
    17761 tggacctgct ggaatgatgg cagctatttc ctcaaatttt tacggcaaca aaacacttct
    17821 tattgagaaa aataaacgtt taggcaaaaa actagctgga acaggtggtg gccgctgtaa
    17881 tgttaccaat aatggaaacc ttgatgagct cttagctggt atacctggaa atggccgttt
    17941 cctatatagc gtcttctcac aatttgataa ccatgatatt attaacttct ttcaagacaa
    18001 cggcgtcaca cttaaagaag aagatcatgg gcgtatgttc cctactactg ataaatctcg
    18061 tactatcatt aacgcccttg aaaataaaat aaaagaactt ggtggtcaaa ttatgactga
    18121 cacagaagtt gtctctgtca aaaagatagg agattcattc tatatcaaaa ctaaagatac
    18181 acagttcgca tccgataaac ttatcgtaac aactggtggt aaatcctatc cgtctacagg
    18241 ttctactggt ttcggtcatg acattgctcg tcactttaaa ttagaagtca cagatatgga
    18301 agccgccgaa agccctcttt taactgattt tccccataaa aaactacaag gtatctcctt
    18361 agatgatgtc actttgtcat ttgaaaagca tattattaca cacgatcttc tctttactca
    18421 ttttggatta tcaggaccag cagccttgcg aatttctagc tttgtcaaag gcggagaaac
    18481 tatataccta gatgttttgc ccaatatctc tgttaaagaa ttggaaatac acttccaaaa
    18541 tgaaagagaa aaatctttga aaaacgctct taaaatactt cttcctgagc gtttagctga
    18601 attttacgct gaagacttac ccgaaaaagt aaaacaagta tctgtaaaag acttagaaat
    18661 gttaattcaa aaacttaaaa aacttcctat tttagtaact ggcaagatgt ctctagcaaa
    18721 atcctttgta acaaaagggg gagttgatct taaagaaata aaccccaaaa cacttgaaag
    18781 taaaaaggta gctggtttac attttgctgg tgaagtccta gacatcaatg ctcatacggg
    18841 agggtttaac attacttctg ccttatgtac aggctgggta gctggtagct tacactatta
    18901 atacatgaaa gctgaacaat tccgtcagct tttttatata gaatttaaag ataatatttg
    18961 gctcttaagg cttctttgat tttttccaat tcatctggta aatttacctg atgaatagca
    19021 tacccaatct gataagggga tactataaaa cgaggaacaa ttttacaaat aagaccccca
    19081 tcatgcttat agaaaaagag attataagat ttacagcgcc ctcccaagaa ttcatttaaa
    19141 atataatgac acaaaattgg taaactatct gcaatcaaaa tagcgttttc caatttggat
    19201 actttaattg tgaactctgt gaaatcaagc ataggacgat tggcaatact aatctctgct
    19261 tgttgactcg tttccgaaca aacctcgata ccatcaaacc aatctaagtt caacgtttca
    19321 taagcatcta cgttttttaa gccaacaatt tggctatgag gatgtcgtaa acttccgcca
    19381 gataagggcc caaaatttcg ataaagaacg accgaacggt aatgaccact ttcttccatt
    19441 aattgccatt ttgacaaaag ataatctaac aattggcgca tctttgactc actataaact
    19501 gaaatatccc cattgtgatc atttgattca atcaccaatg tttgataggt ttcttctaaa
    19561 gttgggtatt tattttctac ccagatcata tcgtctttca cgtctaggat ttttcctaat
    19621 tggcttttat cacaaaatgg acatttgtct ttattgctgg gtttttgttt cgcgatatta
    19681 gggttaaatc ggatatatct ttcattcata ctgttattgt aacacattca tcaccattac
    19741 aatataatac ttatccagtt aacattttcc ttgcattctt agctgaaaag agtataatag
    19801 aatcatgtct tgtttaccgg ttaattacca gttaactagg caccctgttt tgggatagaa
    19861 aaatcttttt gggaaaggag aaatcatggc taagaaatca aaaattgcta aatttcaaaa
    19921 acagcaaaaa ctcgtagaac aatatgctga actacgtcgt gagttaaaag aaaagggtga
    19981 ttacgaagca cttcgtaaac ttcctaaaga ttctaaccct aaccgactta aaaatcgtga
    20041 tttaatcgat ggccgtcctc acgcttacat gcgtaaattc ggtatgagtc gtattaactt
    20101 ccgtaaccta gcttacaaag gccaaattcc tggcattaaa aaagcaagct ggtaattcgt
    20161 gaagacaaat agttactttt tatgactatt ctattgagtg tggacgaata gttaatacat
    20221 tgtaattatt taacctgttg agtatttact cagctattac aaacttct
//
LOCUS       DATRQA010000022        20253 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.022, whole genome shotgun sequence.
ACCESSION   DATRQA010000022 DATRQA010000000
VERSION     DATRQA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20253
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.022"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            29..532
                     /locus_tag="VBD27_000580"
     CDS             29..532
                     /locus_tag="VBD27_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024614.1"
                     /translation="MKLNKIILSTAALTALFLGYNSVTADTYNNYQPHRSNNMDLTEE
                     YNYNNQIELQERIKNLNIPFKEKLALLRKVKSRQDSYVLDILRKEVENKNSEYSELEQ
                     EYQRISALINAKFPQGKSSLAEKLHKELIWDLDGVKYDEGISKRTTLKKLEGKLNEYT
                     KKYSYLF"
     gene            complement(584..770)
                     /locus_tag="VBD27_000581"
                     /pseudo
     CDS             complement(584..770)
                     /locus_tag="VBD27_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            842..1567
                     /locus_tag="VBD27_000582"
     CDS             842..1567
                     /locus_tag="VBD27_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7024615.1"
                     /translation="MLKIENVTGGYVNIPVLKNISFEVNDGELVGLIGLNGAGKSTTI
                     NEIIGILRPYQGDITIDGISLEANQELYRKKIGFIPETPSLYEELTLREHLETVAMAY
                     DIATDEVMARAQKLLEMFRLTDKLDWFPMHFSKGMKQKVMIICAFVVSPSLFIVDEPF
                     LGLDPLAISDLINLLAEEKAKGKSILMSTHVLDSAEKMCDRFVILHKGEIRAVGTLEE
                     LRAIFGDSNANLNDIYIALTKEV"
     gene            1569..2603
                     /locus_tag="VBD27_000583"
     CDS             1569..2603
                     /locus_tag="VBD27_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7024616.1"
                     /translation="MKKLFNKRRSLFLTQNSKYLRYVFNDHFVLVLMFLSGFLLYQYS
                     QLLKDFPKTHWPIIVIVSIIILMLLAMGEIASYLEPADKQFLLIKEEAIKEIINSAKK
                     RTYIFWLVVQTLFLVLISPILIKLGLSVFMITLLIFGLGIIKWLVITYKVKVFYNNQN
                     LNWDAAINHEQERKQAILKFFSLFTNVKGISTSVKRRSFLDVILKLISKTPSRLWTNL
                     FVRAFLRSSDYLGLTIRLVTLNILSVIFVNETYLALALAFVFNYLLLFQLLALGHHFD
                     YQYMNQLYPVRLNAKASQLKGFLRVLSYAVTVIDIILIRELKPVILLIVLMLIVTEYY
                     IPYKIKKMID"
     gene            2661..3461
                     /locus_tag="VBD27_000584"
     CDS             2661..3461
                     /locus_tag="VBD27_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO7024617.1"
                     /translation="MTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPAL
                     AKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLTKEDMGSKQIIHILLRLHKSRPLVNQ
                     LLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVATIVHGDI
                     KHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKV
                     WSKIIWYGQFSYLSQIIKCFDKRDMEHVNQEIYELRKFRELIKKYNAS"
     gene            3451..4086
                     /gene="trmB"
                     /locus_tag="VBD27_000585"
     CDS             3451..4086
                     /gene="trmB"
                     /locus_tag="VBD27_000585"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO7024618.1"
                     /translation="MRVRKRKGAEEHLENNPHYVISNPEEAKGRWHEIFGNNNPIHIE
                     VGSGKGAFITGMAEQNPDINYIGIDIQLSVLSYALDKVLDSGAKNIKLLLVDGSSLSN
                     YFDTGEVDLMYLNFSDPWPKKKHEKRRLTYKTFLDTYKDILPEQGEIHFKTDNRGLFE
                     YSLASFSQYGMTLKQVWLDLHASDYQQNIMTEYERKFSNKGQVIYRVEARF"
     gene            4130..4217
                     /locus_tag="VBD27_000586"
     tRNA            4130..4217
                     /locus_tag="VBD27_000586"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4164..4166,aa:Ser,seq:gga)
     gene            4575..5051
                     /gene="rimP"
                     /locus_tag="VBD27_000587"
     CDS             4575..5051
                     /gene="rimP"
                     /locus_tag="VBD27_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO7024619.1"
                     /translation="MANQTIVDIVTQVVTPAIQAPFELVDVEYEKMGGDYVLSILIDK
                     PGGITVEDTAQLTDVVSPLLDTIQPDPFPEQYMLEVSSPGLERPLKTAEALSNAVGSY
                     INVSLYKSIDKVKIFEGDLLSFDGETLTIDYMDKTRHKTVDIPYQTVAKARLAVKL"
     gene            5078..6238
                     /gene="nusA"
                     /locus_tag="VBD27_000588"
     CDS             5078..6238
                     /gene="nusA"
                     /locus_tag="VBD27_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO7024620.1"
                     /translation="MDTMSKEMLEAFRILEEEKHINKEDIIDAVTESLKSAYKRRYGQ
                     SESCVIEFNEKKADFTVYTVREVVDEVFDSRLEISLKDALAISSAYELGDKIRFEESV
                     TEFGRVAAQSAKQTIMEKMRRQMREVTFNEYKQHEGEIMTGTVERFDQRFIYVNLGSL
                     EAQLSHQDQIPGESFKSHDMIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEREIPEV
                     FDGTVEIMSVSREAGDRTKVAVRSHNSNVDAIGTIVGRGGSNIKKVISNFHPKRVDAK
                     TGLEIPVEENIDVIQWVEDPAEFIYNAIAPAEVDMVLFDDEDTKRATVVVPDSKLSLA
                     IGRRGQNVRLAAHLTGYRIDIKSASEYEKMEAQELQTEEVAQESEVISDKAE"
     gene            6260..6556
                     /locus_tag="VBD27_000589"
     CDS             6260..6556
                     /locus_tag="VBD27_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEO7024621.1"
                     /translation="MAKTKKIPLRKSVVSGEVIDKRDLLRIVKNKEGQVFIDPTGKQN
                     GRGAYIKLDNDEAILAKKKRVFDRSFSMEVSDEFYDELLAYVDHKVKRRELGLE"
     gene            6549..6851
                     /locus_tag="VBD27_000590"
     CDS             6549..6851
                     /locus_tag="VBD27_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEO7024622.1"
                     /translation="MNNSEKVLNLIGLAQRAGRLITGEELVIKAIQNQQVSLIFLAND
                     AGPNLTKKVTDKSNYYKTEVSTVFSTLELSDALGKPRKVVAVADAGFSKKMRTLMA"
     gene            6871..9654
                     /gene="infB"
                     /locus_tag="VBD27_000591"
     CDS             6871..9654
                     /gene="infB"
                     /locus_tag="VBD27_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO7024623.1"
                     /translation="MSKKRLHEIAKEIGKTSKEVVEQAQSLGLPVKSHASSVEENDAT
                     RIVESFSSSKTKAPTNSVQTNQGVKTESKTVETKQGLSDDKPSTQPVAKPKPQSRNFK
                     AEREARAKAEAEKRQHNGDHRKNNRHNDTRSDDRRHQGQKRSNGNRNDNRQGQQNNRN
                     KNDGRYADYKQKPQTRPQQPAGNRIDFKARAAALKAEQNAEYSRHSEQRFREEQEAKR
                     QAAKEQELAKAAALKAQEEAQKAKEKLASKPVAKVKEIVNKVAATPSQTADSRRKKQT
                     RSDKSRQFSNENEDGQKQTKNKKNWNNQNQVRNQRNSNWNHNKKNKKGKTNGAPKPVT
                     ERKFHELPKEFEYTEGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGDTIELL
                     MVDYGIEAHAKVEVDEADIERFFADEDYLNPDNLTERPPVVTIMGHVDHGKTTLLDTL
                     RNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMRARGASVTDITILIV
                     AADDGVMPQTVEAINHSKAAGVPIIVAINKIDKPGANPERVISELAEHGVISTAWGGE
                     SEFVEISAKFGKNIQELLETVLLVAEMEELKADADVRAIGTVIEARLDKGKGAVATLL
                     VQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGDHFAV
                     YADEKAARAAGEERAKRALLKQRQNTQRVSLENLFDTLKAGEVKSVNVIIKADVQGSV
                     EALAASLLKIDVEGVKVNVVHSAVGAINESDVTLAEASNAVIIGFNVRPTPQARQQAD
                     ADDVEIRQHSIIYKVIEEVEEAMKGKLDPEYQEKILGEAIIRETFKVSKVGTIGGFMV
                     INGKVTRDSSVRVIRDGVVIFDGKLASLKHYKDDVKEVGNAQEGGLMIENYNDLKEDD
                     TIEAYIMEEIKRK"
     gene            9744..10112
                     /gene="rbfA"
                     /locus_tag="VBD27_000592"
     CDS             9744..10112
                     /gene="rbfA"
                     /locus_tag="VBD27_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO7024624.1"
                     /translation="MKKGLIMANHRIDRVGMEIKREVNEILRLRVNDPRVQDVTITDV
                     QMLGDLSMAKVFYTIHSTLASDNQKAQIGLEKATGTIKRELGKNLTMYKIPDLQFVKD
                     ESIEYGNKIDEMLRNLDKKD"
     gene            complement(10184..11188)
                     /locus_tag="VBD27_000593"
     CDS             complement(10184..11188)
                     /locus_tag="VBD27_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7024625.1"
                     /translation="MKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWL
                     KRDKSYYKVQDESSFQKYLNASREQSDKGYYLDPNSVNGGLVQERLFDMQVYSWNDNG
                     KANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYDYAIPRL
                     VNLYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVT
                     MKHPEIPKYEDTDPILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTG
                     TREIFFPDIRDYAAQLQAANHPVNYIAQEGMNHVYPIYPIEEAKTAQYQMIDIINKTP
                     "
     gene            11351..11767
                     /locus_tag="VBD27_000594"
     CDS             11351..11767
                     /locus_tag="VBD27_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEO7024626.1"
                     /translation="MTISNAEWEIMRVVWAQQNTTSNEILAVLLEKYDWTPSTVKTLL
                     RRLLDKGYVSREKMGKGFSYSSLIDEDLAMMSEVDSVLQKVCQTKHVAIVRHLLESIP
                     MTEKDRLNLQASLEAKKGKTLERVACNCIPGQCQCH"
     gene            11780..14014
                     /locus_tag="VBD27_000595"
     CDS             11780..14014
                     /locus_tag="VBD27_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO7024627.1"
                     /translation="MANRKETFLIDGMTCASCALTIEKAVNKLDHVDSAVVNLATEKM
                     TVTFDDTTLSPNVIEECVSETGYEASLFKEETSKSQSERHQLAIEKMWHRFWMSAVAT
                     IPLLYISMEPMINLWLPSFLMPDKGPLNYGVIQLLLTLPVMYFGRIFYQNGFKALFKR
                     HPNMDSLVAIATTAAFIYSLYGLYEILQGDIHYAHQLYFESVAVILTLITLGKYFEIL
                     SKGRTSASIEKLLTLSAKEARVIKDGEDYMVPLDKVKIGETILVKPGEKIPLDGHVVA
                     GESSIDESMLTGESIPVEKKVGSKVYGASINGQGSLTIFVEKEAVGSLLSQIINLVEV
                     AQASKAPIANLADKVSGVFVPFVIVIAILSGLSWYLILGQSFAFSLKIMIAVLVIACP
                     CALGLATPTAIMVASGKAAENGILFKGGEVLEKAHHIDTIVFDKTGTLTKGKPEVVAI
                     KTYGVDKEEFLGQVASVEKLSNHPLSQTIVNKAKEKELPLREVMAFKNILGYGLSAII
                     NGKTMLVGNANLMTKNDVNLDLAKADIEIAQEEAQTVVYVSENGVLSGLITLTDQLKT
                     DSQETVKQLQRLGFNLVLLTGDNKASADAIAQKLGITTVVSEVLPDQKANVILELKEK
                     GGQIAMVGDGINDAPALASSDVGISMSSGTDIAIESADIVLMKPELTDLLKAMTISKQ
                     TIQIIKENLFWAFFYNVLAIPVAMGVLHLFGGPLLNPMLAGLAMAFSSVSVVLNALRL
                     KVLK"
     gene            14055..14261
                     /locus_tag="VBD27_000596"
     CDS             14055..14261
                     /locus_tag="VBD27_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEO7024628.1"
                     /translation="MKHTYRVSGMKCDGCAKTVSDKLSSVMGVDEVNVDLTKNQVVVS
                     GKTFKCLLKRSLKDTKYSLEEEIN"
     gene            14371..14985
                     /locus_tag="VBD27_000597"
     CDS             14371..14985
                     /locus_tag="VBD27_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEO7024629.1"
                     /translation="MSIDIWNILSLVGTVAFASSGAIVAIEEEFDVLGLFILGFVTAF
                     GGGAIRNVLIGLPIETLWSQGIAFYAAAAAILFIMIFPNLLSGKGRDAEVVSDAIGLA
                     AFSVQGALYATQSHQPLSAVIVAAVLTGAGGGIVRDVLAGRKPGVLRSEIYAGWSILV
                     GIILYFKIAKTTTDYYLLVLVVTSLRMLGYKKQWHLPVVRPRKK"
     gene            15000..15812
                     /locus_tag="VBD27_000598"
     CDS             15000..15812
                     /locus_tag="VBD27_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7024630.1"
                     /translation="MIKLIAIDMDGTLLNDEKKIPKENIQAIKEATQAGIKIVLCTGR
                     PMSGILPYFNELGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFP
                     NVCLTLTAENTFYAVGEEVPEIVAYDADLVFTKAKSISLDALRNQEEIVFQAMYMGLD
                     ADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAI
                     GDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNQAGVAKAIYDYALR"
     gene            15925..18567
                     /gene="polA"
                     /locus_tag="VBD27_000599"
     CDS             15925..18567
                     /gene="polA"
                     /locus_tag="VBD27_000599"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO7024631.1"
                     /translation="MTNKNKLLLIDGSSVAFRAFFALYNQIDRFKNNSGLHTNAIYGF
                     HLMLNHILGRVQPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPYIRQQLD
                     VLGIKHYELEHYEADDIIGTLAKQAEASKEHFDITVVSGDKDLIQLTDTNTVVEISKK
                     GVAEFEEFTPAYLMEKMGITPSQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLSEYGS
                     LEGIYENIEAMKQSKMKENLINDKEQAFLSKTLATINIASPITIGLEDILYSGPQDIK
                     ALSQFYDEMDFKQFKAALGEETSQEDFEVDFTEVEQLKPEMFSDNDFYYFEMLGDNYH
                     VEDLIGIAWGNSDTIYATSDVSLLQEALFKKALSKPIKTYDFKRSKVLLNRFNIDLPE
                     PAFDTRLAKYLLSTTEDNLVSTIARLYTNLPLDTDDAVYGKGAKRAIPEKTRFLEHLA
                     KKVKVLVDSEANIMQQLKANEQEELLFEMEQPLANVLAKMEIRGIKVKKNTLNEMAIE
                     NQKVIETLTQEIYELAGQEFNINSPKQLGKLLFETLGLPVEMTKKTKTGYSTAVDVLE
                     RLAPISPLVTKILEYRQITKLQSTYIIGLQDYILEDGKIHTRYVQDLTQTGRLSSSDP
                     NLQNIPVRLEQGRLIRKAFVPSEDNAVLLSSDYSQIELRVLAHISKDEHLIAAFKEGA
                     DIHTSTAMRVFGIEKPENVTPNDRRNAKAVNFGIVYGISDFGLSHNLGIPRKLAKQYI
                     DTYFERYPGIKNYMETVVREAKDKGYVETLFHRRRSLPDINSRNFNIRQFAERTAINS
                     PIQGSAADILKIAMINLDRVLDKGGYKSKMLLQVHDEIVLEVPNEEIGAIKELVTKTM
                     ESAISLSVPLIADENAGETWYEAK"
     gene            18597..19037
                     /locus_tag="VBD27_000600"
     CDS             18597..19037
                     /locus_tag="VBD27_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO7024632.1"
                     /translation="MVYHFQNLSDFMLKNYLTKAKTIAVVGLSDRQETAAYQVSKIMQ
                     EAGYQIIPVNPKNAGQKILGQMTYASLKDVTEHIDIVNIFRRSEYLPDIAREFLEVDA
                     DIFWAQLGLESQEAETILKQAGHKQIVMNKCLKVEYQKLHSETN"
     gene            19119..19598
                     /gene="perR"
                     /locus_tag="VBD27_000601"
     CDS             19119..19598
                     /gene="perR"
                     /locus_tag="VBD27_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEO7024633.1"
                     /translation="MDSHSHGHRPLDAYENVLEHLREKRIRITETRKAIISYMVNSRE
                     HPSAEKIYNDLLPEYPNMSLATVYNNLKVLVDEGFVTELKLCNYSTTYYDFMGHQHLN
                     IACEDCGKIVDFVDVDLLDISREAHQQTGFEVTRVQLVAYGICPECQRKRKDKYKKD"
     gene            19751..>20253
                     /locus_tag="VBD27_000602"
     CDS             19751..>20253
                     /locus_tag="VBD27_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO7024634.1"
                     /translation="MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQ
                     PKTTITAPTANTSTNVESSTDKALSKVTTMETSSEMPKMQNMAKVEKTSDKPMMVATS
                     VRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLAGVNQTI
                     PYEAFGG"
BASE COUNT         6862 a         3085 c         4144 g         6162 t
ORIGIN      
        1 tgagatttat catagaagga aaactatttt gaaattaaat aaaatcatat tatctactgc
       61 agctcttact gctctctttt taggatataa tagcgttact gcggatacat ataataacta
      121 tcagccacat agatcaaata atatggattt aactgaggaa tataactata ataaccagat
      181 agaacttcag gagcgtataa aaaacctaaa tatacctttt aaagaaaaac ttgccttact
      241 cagaaaggtt aaatcaagac aagattctta tgtattagac atattaagaa aagaagttga
      301 aaataaaaac agtgagtatt cagaactaga gcaagaatac caaagaattt cggctcttat
      361 taatgctaag tttccgcaag ggaaatctag tttagcagaa aaattacata aagaactaat
      421 ttgggacttg gatggtgtca aatatgatga ggggatttct aagagaacta ctctaaaaaa
      481 attagaaggt aaactcaacg aatatactaa aaaatacagt tatttattct gataattgac
      541 tttggtaatt ttatagccta atattctgat tactttgcat ttattatatt gatactaaaa
      601 agacaccttt ttgaggtgtc tttttttgtc cgatgagtga tggaggtgct ttttgtaaac
      661 ataatgggag catggggtag cttggcattt caattagatg taaaagcgga caaaataggt
      721 ttaaagttat tttcaaaact ataagggaat tctaagatac aattatccat aagaatagac
      781 cttccctttc ttattttgtt ataatagtta ataaagtata tcaaagatag agagaatgac
      841 aatgttaaaa attgaaaatg ttacaggggg gtatgttaat atcccagttt taaaaaatat
      901 ttcttttgaa gtaaatgatg gagagttagt tggattaatt gggcttaatg gtgcaggaaa
      961 atcaaccact attaacgaga ttattggtat tttgagaccc tatcaaggag atattaccat
     1021 tgatggcatt agtcttgaag ccaaccaaga actttataga aagaaaatag gctttattcc
     1081 tgagacacca agtctctatg aggaactaac tctcagagaa catttagaaa cggtagctat
     1141 ggcgtatgat attgccaccg atgaagttat ggcacgtgcg caaaaactat tagagatgtt
     1201 tcgtctaaca gataaattag actggtttcc aatgcatttt tcaaagggaa tgaaacaaaa
     1261 ggttatgatt atctgtgctt ttgttgtatc tccaagttta tttattgttg atgaaccatt
     1321 tttaggtttg gatccactag ctatttcaga tttgattaat cttctagcag aagaaaaagc
     1381 taaagggaaa tctattttga tgtcaacaca cgtattagat tctgccgaaa aaatgtgtga
     1441 tcgttttgtt attttacata aaggagaaat acgggcagtt ggaaccctag aggaattacg
     1501 ggctattttt ggagacagta atgctaattt aaatgacatc tacatagctc tgacaaaaga
     1561 ggtgtgatat gaaaaagtta tttaataaac gtcgaagtct ctttttaact caaaatagta
     1621 aatatttgag atacgtcttt aatgatcatt ttgtattagt attaatgttt ttgtcaggtt
     1681 ttttgttata tcagtatagc cagttactaa aagactttcc aaaaacacat tggccaatta
     1741 tcgtgattgt gtcaatcatt atcttaatgt tactagcgat gggggaaata gccagctatt
     1801 tagaaccggc tgataaacaa tttttactta taaaagaaga agcaataaag gaaattataa
     1861 attctgctaa aaaacggact tatatctttt ggttggttgt tcaaacctta tttttagtat
     1921 taatatcacc aattttgata aagttaggct tgtcagtttt tatgattaca ttgttaattt
     1981 ttggtctagg aataatcaaa tggttggtga taacttacaa ggtaaaggtc ttttataaca
     2041 atcaaaattt gaactgggat gcagctataa atcatgagca ggaaagaaaa caggctatat
     2101 taaaattttt ctctttgttt acaaatgtta agggaatttc gacaagtgta aaacgtcgtt
     2161 catttttaga tgttatttta aaacttattt ctaaaacgcc atcgcgttta tggactaact
     2221 tatttgtgag ggcatttcta agaagctcag attatcttgg tttaaccata aggttggtaa
     2281 ctttaaatat cttatcagtc atctttgtta acgaaaccta cctcgccctt gctctggcat
     2341 ttgtatttaa ctacttatta ttgttccaat tattagcttt aggtcaccat tttgattatc
     2401 aatacatgaa tcagctctat ccagttagat taaatgctaa agcaagtcag ctaaaaggtt
     2461 ttctcagagt gttatcatat gctgtgacag taattgatat catacttata agagaattga
     2521 aacctgttat attattgata gttttgatgt taattgtgac ggaatattac atcccttaca
     2581 aaataaagaa gatgattgac taaagcctat aaaacaagta aaataagagg aaacgactaa
     2641 atgaaaagga gggactgctg gtgacaatat caaatcaaga attgacgctt actcccttaa
     2701 gaggaaaaag tggcaaagcc tatattggta cttatccaaa tggtgaacgt gtctttgtca
     2761 aatataatac gacacctatt ttgccagcct tagctaagga acagattgcg ccacaactac
     2821 tgtgggcacg ccgtacaagt aatggcgata tgatgagtgc tcaagaatgg ttagatggtc
     2881 gaacattgac taaagaagat atgggtagta aacagattat tcatatttta ctgcgcctgc
     2941 acaagtctcg tcccttagta aatcaactgt tacaattagg gtataaaatt gaaaaccctt
     3001 atgatttgtt gatggattgg gaaaaacaaa ctcctattca aatccgagaa aacacttatt
     3061 tgcaatctat tgtgactgag ttaaagcgta gtttacctga gtttcgaaca gaggttgcaa
     3121 caatcgttca cggagatatc aaacatagca actgggttat tacaacaagt ggtttaattt
     3181 atttggttga ttgggattct gttcgtttaa cagatcgcat gtatgacgtt gcatatattt
     3241 taagtcatta tattccacaa aaacactgga aagattggct gtcttactac ggttataaag
     3301 ataatgagaa agtctggagc aaaattattt ggtatgggca attttcatat ttatcacaga
     3361 taatcaaatg ttttgataaa cgagatatgg agcatgtcaa tcaagaaatt tatgaattac
     3421 gaaagtttag agagttaatt aaaaagtata atgcgagtta gaaaacgtaa gggtgcagag
     3481 gaacacttag aaaataatcc gcactatgtt atttcaaatc cagaagaagc caaagggcgt
     3541 tggcatgaga tttttggaaa taataacccc attcacatag aagttggttc cggaaaaggg
     3601 gcttttatca ctggtatggc agagcaaaat ccagatatca attatattgg aattgatatt
     3661 caattgtcag tgctttccta tgctctggat aaagttctag atagcggtgc taagaatatc
     3721 aaattactat tagttgatgg ttctagtcta tcaaactact ttgatactgg cgaagttgat
     3781 ttgatgtatc ttaactttag tgatccatgg cctaagaaaa agcatgagaa acgtcgattg
     3841 acctataaga ctttcttgga tacctataaa gacatacttc ctgagcaggg ggaaattcat
     3901 tttaaaacag ataatagagg tttatttgaa tatagccttg ctagcttttc tcaatacggt
     3961 atgactttaa aacaagtttg gttagattta catgcaagtg attaccagca aaatatcatg
     4021 actgagtacg aacgcaaatt ttcaaataaa ggacaggtca tctatcgtgt agaagcacga
     4081 ttttagaata agtaaatagt aagtgggtag ttatttgcta ctcctttatg gagaggtcgc
     4141 atagtggctg agtgcgcacg cttggaaagc gtgtagtccc taatgggggc tcgggggttc
     4201 gaatcccctc ctctccttta atcttgttaa tgtatcagtg tttattaagt gttcaccttt
     4261 ttcactcgtc aaaagtccaa gtttcgaaaa atttctttta ttttatcatc gtttctcttt
     4321 gagcttgaag tattagcata aagttttcta agctcttaca aagaattgag gacaaatttt
     4381 aattttttga tataagatta gtgtacggtg atgctaattt aaagtaaatg attgcaataa
     4441 tcgtattttg tgctataata gtattaaata atacagaaag gggcggagtt ttacttcgcc
     4501 tcttatgtgt gtgattcata gaatttatag tcattgtaag ttctaaagca agttagaaag
     4561 aagaggtagc tgttatcgca aatcaaacca ttgttgatat tgttactcaa gtcgtaactc
     4621 ctgctattca agcaccgttt gaattagtag atgtagaata cgaaaaaatg ggtggcgact
     4681 atgtgctcag tattttaatt gataaacccg gaggcattac ggttgaagat acagcacaat
     4741 tgacagacgt tgtaagtccc ttacttgaca caattcaacc agatccattt ccagaacagt
     4801 atatgcttga agtctcaagt cctgggttgg aaaggccact taaaacagca gaagcattat
     4861 caaatgctgt tgggtcttat atcaatgtaa gtctgtataa atccattgat aaagttaaaa
     4921 tatttgaagg tgacttatta tcatttgatg gtgaaacctt gacaattgat tatatggaca
     4981 aaacacgtca taaaactgta gatattccct atcagacggt tgcaaaagcc cgtcttgcgg
     5041 ttaaacttta agaaaggatg attcttgcga agcaagaatg gacactatga gcaaagaaat
     5101 gctagaagcc ttccgtattt tggaagaaga aaaacacatt aataaagaag atattattga
     5161 tgcagttact gagtcactaa aatctgccta taagcgtcgt tatggtcaat cagaatcatg
     5221 cgttattgaa tttaacgaga agaaagcaga ctttaccgtc tatactgttc gtgaagttgt
     5281 tgatgaagtt tttgatagcc gtttggaaat tagcctaaaa gacgcacttg ctattagctc
     5341 agcgtatgaa cttggtgata agattcgctt tgaggaatct gttacagaat ttggacgcgt
     5401 ggcagctcag tcagcaaaac aaactattat ggaaaaaatg cgtcgtcaaa tgcgtgaagt
     5461 aaccttcaat gaatacaaac aacacgaagg tgaaattatg actggtactg ttgaacgatt
     5521 tgaccaacgt tttatctatg tcaatctcgg ttcactcgag gcgcaattat cgcatcaaga
     5581 tcagattcct ggggagagct ttaaatctca cgatatgatt gacgtttatg tctataaagt
     5641 ggagaataat cctaaaggtg tcaacgtttt tgttagccgt agtcatccag agtttatcaa
     5701 acgcattatg gagcgagaaa ttccagaagt atttgatgga actgttgaga ttatgagcgt
     5761 ctctcgtgag gctggagatc gtactaaagt tgctgtacgc agtcataatt caaacgttga
     5821 tgcgattgga acaattgttg gacgcggtgg aagtaacatt aaaaaagtta tcagtaattt
     5881 ccatcctaaa cgcgttgatg ctaaaacagg attggagatc ccagttgaag aaaacattga
     5941 tgttattcaa tgggttgaag atccagcgga attcatttac aatgctattg caccagccga
     6001 agttgatatg gtactttttg atgatgaaga tacaaaacgt gctactgtag ttgttccaga
     6061 tagcaagtta tcgctagcta ttggtcgccg aggacaaaat gtgcgtttag cagctcattt
     6121 gactggctat cgtattgata ttaaatctgc aagtgaatac gaaaaaatgg aagcgcaaga
     6181 acttcaaact gaggaagtag ctcaagaatc tgaagttatt tcagataagg cagaataata
     6241 taaatgagag aggtgtttta tggctaaaac aaaaaaaata cctttaagaa aatcagttgt
     6301 ttctggtgaa gtgatcgata agcgagatct tttacgaatt gttaaaaata aagaaggaca
     6361 ggtttttata gatcctaccg gaaagcaaaa tggtcgaggt gcctacataa aattggacaa
     6421 tgatgaagct atcctagcga aaaagaaacg agtgtttgat cgcagttttt caatggaagt
     6481 ttctgatgaa ttctatgatg aactattggc atatgtcgac cataaggtca aaagaagaga
     6541 gttaggcctt gaataattca gaaaaagtat taaatctcat tgggttagca cagcgtgcgg
     6601 ggcgccttat tacaggtgaa gaattggtta ttaaagcaat tcaaaatcaa caagtatcgc
     6661 taatctttct agcaaatgat gctggcccaa atttgacgaa aaaagtaact gataaaagta
     6721 attactacaa aacagaagtc tccacagtgt tttcaacact ggaattaagt gatgcgttag
     6781 gcaaaccaag aaaagtggtt gctgtcgcag acgctggatt ttcaaagaaa atgaggactc
     6841 ttatggcata acagaatagg aggacatcaa ttgtcaaaga aaagattaca cgaaatcgct
     6901 aaggaaattg gaaaaacgag taaggaagtg gttgagcaag ctcaatcgct tggcttacct
     6961 gtaaaaagtc atgcttctag cgttgaagaa aatgatgcta cacgtattgt tgagagtttt
     7021 agctcttcta aaactaaagc tccaacaaat tctgttcaaa ctaatcaagg tgtgaaaaca
     7081 gaatctaaaa ctgttgaaac caaacaaggc ttgtccgatg ataaaccttc aactcaacct
     7141 gtagctaaac cgaaaccaca aagtcgtaac tttaaggcgg agcgcgaggc tcgtgctaaa
     7201 gctgaagcgg agaagcgtca acataatggg gatcaccgta agaataatcg ccataatgat
     7261 acacgctctg acgacagacg ccatcaaggt caaaagcgtt caaatggtaa tcgtaatgat
     7321 aaccgacaag gtcagcaaaa taaccgtaat aaaaatgatg ggcgttatgc tgattataaa
     7381 cagaaacccc aaacgcgtcc tcagcaacct gcaggcaatc gcattgactt taaagctcga
     7441 gcagcagctt tgaaagctga gcaaaatgct gaatactcac gtcatagtga gcagcgtttc
     7501 cgtgaagagc aagaagccaa acgtcaagca gctaaggagc aagaactagc taaagcagca
     7561 gcgttaaaag cgcaagaaga agctcaaaaa gctaaggaaa aacttgcttc taaacctgtt
     7621 gctaaggtaa aagaaattgt caataaagta gccgctacgc catcacagac agcagatagt
     7681 cgtcgtaaaa aacaaactcg ttctgataag tctcgtcaat tttcaaatga aaatgaagat
     7741 ggtcaaaagc aaacaaaaaa taagaaaaac tggaataatc aaaaccaagt gagaaatcaa
     7801 agaaatagta actggaatca taataagaaa aataaaaaag gtaagactaa tggagctcca
     7861 aaaccagtaa cagagcgtaa attccatgaa ttaccaaaag aatttgaata tactgagggc
     7921 atgaccgttg cggaaattgc aaaacgtatt aaacgcgaac cagcagaaat tgttaaaaaa
     7981 ctctttatga tgggagttat ggcaacgcag aatcaatcat tagatggaga cactattgag
     8041 ctattgatgg ttgactacgg gattgaagca catgctaagg ttgaagttga tgaagctgat
     8101 attgaacgct tctttgcaga tgaggattac cttaatcctg ataacttaac ggaacgtccg
     8161 cctgttgtta ctatcatggg acacgttgac catggtaaaa caactcttct tgatactcta
     8221 cgtaattcac gtgtcgctac tggcgaagct ggtggaatta ctcaacatat tggtgcttat
     8281 cagattgaag aagctggcaa gaaaattact ttcttggata caccagggca tgcggccttt
     8341 acttctatgc gtgcgcgtgg tgcatcagta actgatatta ctatccttat cgttgccgct
     8401 gatgatggtg tcatgccgca gacagttgag gcaattaatc actctaaagc tgcgggtgtc
     8461 ccaattattg ttgctatcaa caaaattgat aaacctggtg caaatccaga acgcgttatc
     8521 agtgaattag ctgagcatgg tgtcatctct actgcatggg gtggtgagtc tgaatttgtt
     8581 gaaatttcag ctaagtttgg taaaaatatt caagaattac ttgagacagt tcttttagtt
     8641 gctgaaatgg aagaattaaa agcagatgca gatgttcgtg ctattggtac tgttattgaa
     8701 gcccgtcttg ataaaggtaa gggagccgtt gcaacgcttc ttgtccaaca aggtacttta
     8761 aatgtacaag atccaatcgt tgttggtaat acatttggac gtgttcgtgc gatgactaat
     8821 gatcttgggc gtcgtgtcaa agttgcgggt ccatctacac cagtttctat tactggtttg
     8881 aacgaagctc caatggctgg tgatcatttt gctgtttatg cagatgagaa agctgcgcgt
     8941 gctgcaggtg aagaacgtgc aaaacgtgct ctcttaaaac aacgccaaaa cacgcaacgt
     9001 gttagcctcg agaacttatt tgataccctt aaagctggtg aagttaagtc agttaatgtt
     9061 attatcaaag ctgatgtgca aggttctgta gaagcacttg cagcttctct ccttaagatt
     9121 gatgttgaag gagtaaaagt taatgttgtt cactcagcag ttggtgctat taatgagtct
     9181 gatgtaactc ttgctgaagc atcaaatgct gttattatag gatttaacgt acgccctact
     9241 ccacaagctc gtcaacaagc cgatgctgat gatgtcgaaa ttcgtcaaca tagcattatc
     9301 tataaagtta tcgaagaagt tgaagaagcg atgaaaggta aacttgatcc tgaataccaa
     9361 gaaaaaatcc ttggtgaagc cattattcgt gagaccttta aagtttcaaa agtgggaact
     9421 attggtggat ttatggttat caatggtaaa gttactcgtg attcaagtgt tcgtgttatc
     9481 cgtgatggtg ttgtcatttt tgatggtaaa ttagcaagtc ttaaacacta taaagatgat
     9541 gttaaagaag tcggcaatgc tcaagaaggt ggtctaatga ttgagaatta taatgatctc
     9601 aaagaagatg acactattga agcatacatt atggaagaaa ttaaacgtaa ataaccccta
     9661 tgagaaaccc aacttgtatc tttagttggt agggaataca aaattatggc taggctcgtc
     9721 ctagctttca agttatgagc acaatgaaga aaggattaat tatggctaat catcgtattg
     9781 atcgcgttgg aatggaaatt aaacgtgaag tcaatgagat tttgcgtctc cgagttaatg
     9841 atccacgtgt acaagatgtc acaattactg atgttcagat gttgggtgac ctttcaatgg
     9901 ctaaagtatt ttatactatt cattcgacac ttgcttctga taatcaaaaa gcacaaattg
     9961 gtttagaaaa agcaactggt actattaagc gtgaacttgg caaaaacctt actatgtata
    10021 aaataccaga tttacaattt gttaaagatg aatctattga atacggaaat aagattgatg
    10081 aaatgttgcg aaatctagat aaaaaagatt aagaataata aaaaataaac catgtcaaaa
    10141 tcatggttta ttttttatag ctttagagtt gagatagcta gtattaagga gttttattga
    10201 tgatatcaat catttgatac tgtgctgttt tagcttcttc aatagggtaa attggataaa
    10261 catgattcat tccctcttgt gcaatatagt taacggggtg attagctgct tggagttgtg
    10321 cagcataatc gcgaatatct gggaagaata tttctcgtgt tccagtaaat aacgtaatag
    10381 gagcgagttt agtagcaggg gcatttttag gactaacata tgtatggtta gtattattag
    10441 aaccatttgc ccaaatttca cctacacgtg cgagacccca agcagatagg ataggatcgg
    10501 tatcttcgta tttgggaatt tctggatgtt tcattgtcac atctagccat ggagataaga
    10561 ggataatatt tttaggttga ggaatagcct cttgccctga ttggtgagac aatgcatgcg
    10621 ctaatcctag tgctaatcct ccgccagctg aatcacccat caaagtgaga ttagcgtttt
    10681 tttcatgaaa gtgtctataa aggttaacca atctagggat agcataatca taggtataac
    10741 gaggagtttt tggataaatt ggtaaaataa tttttgcatc aagactagtt gagagcgtct
    10801 taagcatgct gatatggtaa ggcgttggat tatttaagta agagccacca gctaaataga
    10861 aaatggtctt ttgattagct ttaccgttgt cattccaaga atagacttgc atatcaaaaa
    10921 gtctctcttg aacaagccct ccattaacag agtttgggtc taagtaataa cccttatcag
    10981 attgttcacg gctagcattt aagtactttt gaaatgagct ttcatcttga actttataat
    11041 aacttttatc gcgctttagc caaaagtatt gttcgataaa ccaacttttc catgaacgcc
    11101 cacttgcttt tgcatgtggt gtacaggcga tggataaaat ggtgataata ctaaatagta
    11161 gcaatagttt tttgaaggac ggtttcaagg tattctcctt aatataatgt tatttttgtg
    11221 taatattatt ataataaaac caacatcttt gtcaatagaa taaagaaaga tgagaaatgt
    11281 ttttcggttt acaaatgtag atttatttgc taaaattggt ttacaaacgt aaatcgaaag
    11341 aggtgagaaa atgactattt caaacgcaga atgggaaatt atgagagtgg tttgggcaca
    11401 gcagaataca accagtaatg agatactagc agttttacta gagaagtatg attggactcc
    11461 ttcaactgtc aaaaccttat taagaaggct cttagataaa ggctacgtga gcagagaaaa
    11521 aatgggaaaa ggatttagct attcttcttt aattgatgaa gatttagcta tgatgtcaga
    11581 ggtagatagt gtattgcaga aagtttgcca gaccaaacat gttgctattg ttagacactt
    11641 attggaaagt ataccaatga cagagaagga tcgattaaat ttgcaagcat cactagaagc
    11701 taagaaaggc aaaacacttg aacgtgtagc ttgtaattgc attcctggtc agtgtcaatg
    11761 ccattagaaa ggagaaaata tggctaatag aaaagaaaca tttttgattg atgggatgac
    11821 ttgtgcatct tgtgctttaa cgatagaaaa agctgtcaac aagttagacc atgtggatag
    11881 tgcagtagta aatttagcaa cagagaaaat gacagttacg tttgatgata caaccttatc
    11941 gccaaacgtg attgaagagt gtgtttctga gactggatac gaagcatcgc ttttcaaaga
    12001 ggaaacttct aaaagccaat ctgaacggca ccaattagca attgaaaaga tgtggcatcg
    12061 cttttggatg tcagcagttg caactatacc tcttctctat atttccatgg agccaatgat
    12121 taacttatgg ttaccatcat ttttgatgcc agataaggga cctctaaact atggtgtgat
    12181 acaattattg ttaacactac cggtgatgta ttttgggcgt atcttttacc aaaatggttt
    12241 taaagcactt tttaaaaggc atcccaatat ggattcatta gttgctatag ccacaacagc
    12301 ggctttcatt tatagcttat atggattgta cgaaatatta cagggtgaca tacactacgc
    12361 tcaccagctt tatttcgaat ctgttgcagt tattttaaca cttattactc taggaaaata
    12421 ttttgaaata ttatccaaag ggcgtacttc ggcctcaatt gaaaaattat taactttatc
    12481 agcaaaagaa gcaagagtta tcaaagatgg tgaagactat atggttcctt tggataaagt
    12541 taaaataggt gaaacaattc ttgtcaaacc tggagaaaaa attcctcttg atggccatgt
    12601 tgtagcagga gaatcaagta ttgatgaatc catgttaaca ggtgaaagta ttccagttga
    12661 aaaaaaggtt ggtagtaagg tttacggtgc cagcattaat gggcaaggta gcttaacgat
    12721 tttcgttgaa aaagaagctg tcggttccct tctatcacaa attattaact tagtagaagt
    12781 tgcacaggca agtaaggctc caattgctaa cttagcagac aaagtatcag gagtctttgt
    12841 cccttttgtt attgtcattg caattttatc aggtttgagt tggtacttaa ttctggggca
    12901 aagttttgcc ttctctctga agattatgat tgcagtctta gtgattgctt gtccatgtgc
    12961 tttagggtta gcaactccta cagctattat ggtggctagt ggtaaagcag ctgagaatgg
    13021 tattttattt aaaggtggtg aagtgttgga aaaggctcac cacattgata caatagtttt
    13081 tgataaaaca ggaaccttga ccaaagggaa accagaagta gttgctatca aaacatatgg
    13141 ggtagataaa gaggagtttt taggtcaagt agcatcagta gaaaaacttt caaatcatcc
    13201 acttagccaa acaattgtta acaaagcaaa agagaaagaa cttcccttaa gggaagtcat
    13261 ggcttttaaa aatattttgg gttacggttt gagtgcaatt attaatggta aaactatgct
    13321 tgtaggtaat gctaacttaa tgactaagaa cgatgttaat ttagatttag caaaggctga
    13381 tatagaaata gctcaagaag aagcacaaac tgtggtttac gtttccgaaa atggagtgtt
    13441 gagcggttta ataactttaa cggaccagct aaagacagat agccaagaaa cagttaagca
    13501 gttacaaaga ttaggattta atttagtttt acttactggt gataataagg ctagtgcaga
    13561 tgccatagca caaaaattgg gtatcacgac agttgtttca gaagttttac ctgatcagaa
    13621 agcaaacgtt attttagaac ttaaagaaaa aggcggacag attgcgatgg ttggtgatgg
    13681 catcaatgat gctcctgcgc tagctagcag tgatgtaggt atctctatga gttctggaac
    13741 agatattgct attgaatcag cagatatcgt gttgatgaaa cctgagttaa cagatctctt
    13801 aaaagcaatg actatcagta aacaaacgat tcaaattatt aaggaaaatt tattttgggc
    13861 tttcttttat aacgttttag cgattccagt ggcaatgggt gttttacatc tttttggtgg
    13921 tcccttactc aatcctatgt tggcaggctt agctatggcg tttagttcag tttcagtagt
    13981 tttaaacgca cttcgcttaa aagtcttaaa ataataaaga taataagact gaatttttat
    14041 gaagggagaa gaatatgaaa catacctatc gagttagtgg catgaaatgt gatggttgtg
    14101 ccaaaacagt ttctgataag ttatcaagtg tgatgggtgt tgacgaggtt aatgttgatt
    14161 tgactaaaaa tcaagttgtc gtaagtggaa aaacattcaa atgtctttta aaacgttctt
    14221 taaaagacac aaagtattca ctagaagagg aaataaatta ataactaaag aagtggttac
    14281 agcttttaat ttcttaattt gagcagtttc tcacttcttt tttattctaa aaatgataaa
    14341 ctagaataga attagttgtg aggagtgcag atgtcaatcg atatttggaa cattttaagt
    14401 ttggttggaa ccgtagcttt tgcaagttcg ggagctattg tggcgattga ggaagaattt
    14461 gatgttctag ggctttttat cttaggtttt gtaacggcat ttggtggtgg agctattaga
    14521 aatgtactaa ttggcttgcc tattgaaaca ctttggagtc aaggaattgc tttttatgca
    14581 gcagcagcag ctattttgtt tattatgatt ttccctaact tgttgagtgg taagggacgg
    14641 gatgccgaag ttgtatcaga tgctattggg ttagcagcgt ttagtgttca aggagcacta
    14701 tatgcgactc aatctcatca acctcttagt gctgtcattg tcgcagcagt actaactggt
    14761 gcaggtggtg gtattgtcag agatgttctg gctggccgaa aacctggggt actaagaagt
    14821 gaaatatatg caggttggtc aatattagtg gggatcattt tatattttaa aattgctaaa
    14881 acaacaactg actattattt gttagtgtta gttgtaacta gcctaaggat gttggggtat
    14941 aagaaacaat ggcacttgcc agttgtcaga ccacgtaaga aatgatagag gagaaagaga
    15001 tgataaaatt aattgctatt gatatggatg gaacgctttt aaatgatgaa aagaaaattc
    15061 caaaggaaaa tatccaagct attaaagaag caactcaagc aggtattaaa attgtactgt
    15121 gtactgggcg accgatgtca ggtatacttc cttattttaa tgaactaggt ttaacaaaag
    15181 aggagtatat tatcatgaat aatggttgta gcacttatag cacgaaagat tggcaattga
    15241 ttgattctgc aactttaaca catgatgaac tcatctttct tgaggaggtt gttaaagaat
    15301 ttcccaacgt ttgtttaacg ttaacagctg aaaatacatt ttacgctgtt ggtgaggaag
    15361 ttcctgaaat tgttgcttat gatgctgatt tagtttttac aaaagcaaag tcaataagtt
    15421 tagatgctct tcgcaatcaa gaagaaattg tctttcaagc aatgtacatg ggattagatg
    15481 cagatgtaac tgcttttcaa gaagctgttg aagaagcctt aatctcgaaa tttagtggag
    15541 ttcgaagcca agactatatt tatgagatta tgcctcaagg agtaacaaaa gcaagaggct
    15601 taaaatcctt gatagcaaaa ttaggactgg atattaatca agttatggct attggtgatg
    15661 ctcctaacga tattgaatta ttagatttag ttcccaatag cgttgctatg gggaatgcat
    15721 cagatgagat taaatctaga tgtaaatata taacggtgga taataaccaa gccggggttg
    15781 ctaaagctat ttatgactat gctttacgtt aaaatagata tagaataata aaaggaactt
    15841 taaacactgt ttgtttaggg ttcttttgtt taataaacta acaaaattca gaagtttaca
    15901 gatagcttat gctataatag acctatgaca aacaagaata aattactttt aattgatggc
    15961 tcatctgtag cctttcgtgc tttttttgct ctttataatc aaattgaccg ctttaaaaac
    16021 aattctggac ttcatactaa tgccatatat ggtttccatt tgatgttgaa tcatatttta
    16081 ggtcgtgtcc agcctagtca tattttagtg gcttttgatg ctggaaaaac tactttccgt
    16141 acggaaatgt atgcagatta taaaggtggg cgtgctaaaa ctccggatga atttcgtgaa
    16201 caatttcctt atattcgtca acaattagat gtcttaggca tcaaacatta tgaattggaa
    16261 cattatgaag ccgatgatat cattggtact cttgcaaaac aagccgaagc tagtaaagag
    16321 cattttgata taacggtggt ttctggggat aaagatttaa ttcaattaac agacactaac
    16381 acggtagtag aaatctctaa aaagggagtt gctgagtttg aagaattcac gccagcttac
    16441 cttatggaaa aaatgggaat tacgccgagt caatttattg atttaaaagc tttgatggga
    16501 gataaatcag ataatattcc tggggttact aaaatcggtg aaaaaactgg cttgaagttg
    16561 ctatcagaat atggttcctt agaagggatc tatgaaaata ttgaggctat gaaacagtct
    16621 aagatgaaag aaaaccttat caatgataag gagcaagctt tcttatcaaa gaccctagct
    16681 acaatcaaca tagcatcacc tataacaatc ggcttagaag atatccttta tagtggaccg
    16741 caagatataa aagccttgtc tcaattttat gatgagatgg attttaaaca gtttaaagca
    16801 gctctgggtg aggagacaag tcaggaagat tttgaggttg actttactga agtggagcaa
    16861 ttaaaaccag aaatgttctc cgataatgat ttttattact ttgagatgtt aggtgataac
    16921 taccacgtag aagatttaat tgggatagct tggggaaata gcgatacaat ctacgctact
    16981 agtgatgtat cgttattgca agaagcttta tttaaaaaag ctttatcaaa acctatcaaa
    17041 acttatgatt ttaaaagaag taaggtcctt ttaaatcgtt ttaatattga tttaccagag
    17101 ccagcctttg atactagatt ggccaagtac cttttgtcta ctactgagga taatctagta
    17161 tcaacaattg ctaggttata tacaaatctg ccactggata ctgatgatgc tgtttatggg
    17221 aaaggtgcta aacgcgctat accagaaaaa acacgatttc ttgagcatct agctaaaaaa
    17281 gtaaaagttt tagtggattc tgaggctaat attatgcaac agcttaaagc taatgagcaa
    17341 gaagaattgc tgtttgaaat ggagcaacct ttagcgaatg ttttagctaa aatggaaatt
    17401 cgaggtatta aggttaagaa aaataccttg aatgaaatgg ctattgaaaa tcaaaaagtc
    17461 atcgaaacct taacacaaga gatctatgaa ttggctgggc aagagtttaa tattaattct
    17521 ccaaaacagt taggaaagtt gttgtttgaa acgttaggtc ttcctgtaga aatgactaaa
    17581 aaaacaaaaa caggttattc aactgcagta gatgtattag agcgcttagc tccgatttct
    17641 cctcttgtaa ctaaaatttt agagtaccgt caaatcacta aattgcaatc aacctatatt
    17701 attggtttac aagattatat tttagaagat ggtaaaattc atacacgtta tgttcaagat
    17761 ttgactcaga caggtcgtct ttcaagttct gacccaaacc ttcaaaatat accagttaga
    17821 ctagagcaag gacggcttat ccgaaaggct tttgttccat cagaagacaa tgctgtttta
    17881 ttgagttcag attattctca aatagaattg cgcgtgttag cacatatttc aaaagacgaa
    17941 catttgatcg ctgcctttaa agagggagca gatattcata cttcaacagc tatgcgtgtt
    18001 tttggtatcg aaaaacctga gaatgtcact cccaatgatc gtcgaaatgc taaggctgtt
    18061 aactttggaa ttgtttatgg tatttcagat tttggtctat cgcataattt aggaatacct
    18121 cgtaaactag cgaagcaata tattgacacc tattttgagc gttatcctgg aataaaaaac
    18181 tatatggaaa cagttgttcg tgaagcaaaa gataagggtt acgttgaaac acttttccat
    18241 cgtcgtcgtt cactaccaga tattaattct cgtaatttta acattcgcca atttgcagag
    18301 cgcacagcaa tcaattcacc aatccaagga agtgcagctg atattcttaa aattgcaatg
    18361 ataaatttag atagggtact tgataaaggt ggttataaat caaaaatgct gttgcaagtt
    18421 catgatgaaa ttgtacttga agtccctaac gaggagatag gagcaatcaa ggagttggtt
    18481 acaaaaacaa tggagtcagc aattagttta tcggtacctt tgattgcaga tgagaacgca
    18541 ggtgagactt ggtatgaagc gaagtagtgg acaacatacg taaaggagtc attaatatgg
    18601 tttatcattt tcaaaatcta agtgatttta tgttaaaaaa ttatttaaca aaagcaaaga
    18661 ccatcgcagt tgtaggattg tcagaccgtc aagaaactgc agcttatcaa gtatcaaaaa
    18721 taatgcaaga agcaggttat caaattattc cagttaatcc taaaaatgca ggacagaaaa
    18781 tacttgggca aatgacatat gcctcactta aggatgtcac tgaacatatt gatattgtta
    18841 atatttttcg acgtagcgag tatttacctg atattgcaag agaattttta gaagtagacg
    18901 ctgatatttt ctgggctcag cttggacttg aaagtcaaga agcagagaca attctaaagc
    18961 aagcaggaca caaacagatt gtcatgaata aatgtctgaa agttgaatac caaaaattac
    19021 actcagaaac taactaatta ccgtgtttaa taggcaaata atataaatta tgctataata
    19081 accatgctaa attaaagttc gtgctggaaa ggagtcctat ggacagtcat tcacatggtc
    19141 atagacctct cgatgcttat gaaaacgtcc tagagcatct ccgtgaaaaa cgtattcgaa
    19201 ttactgagac tcgaaaagct attatttcct atatggttaa tagtcgtgag catccgagtg
    19261 cagaaaagat ttacaatgat ttattaccag aatatcctaa catgagcttg gcaacagttt
    19321 acaataacct taaggtcctt gtggatgagg ggtttgtgac agagctaaaa ctttgcaatt
    19381 atagtacaac ttattatgat tttatggggc atcagcacct aaacattgcc tgtgaagatt
    19441 gtgggaaaat cgttgatttt gtcgatgttg acttattgga tatttctaga gaagcacacc
    19501 aacaaacagg ttttgaagtg actagagttc agttagtagc ttatggtatc tgtccggagt
    19561 gtcagaggaa gcgtaaggat aaatataaaa aagattgaaa cgtaattgtt caatcttttt
    19621 ttgtttctag actaagagaa aaagtttaag ttttactaag taataattaa gaaataattt
    19681 ataattgctt aaaaaatgat ttattttttg ttggtatact ctcattaaca aaaagaaaga
    19741 ggaatccctt atgaaaatat cacaatacaa taaatggtca attcgccgat tgaaagttgg
    19801 agctgcatct gtaatgattg ctagtggaag cattgttgct ttagggcaat ctcatattgt
    19861 ttcagcagat gagatgtccc aacctaaaac aaccattaca gccccaacag ctaacacttc
    19921 tacaaatgta gaaagtagta ctgataaggc attatcaaaa gttactacga tggagacaag
    19981 ttcagaaatg cctaaaatgc aaaatatggc aaaagttgaa aaaacgagtg acaaacctat
    20041 gatggtggct acctctgtta gaaaaatgat ggcaacgccg acacctgtag cgatgaccaa
    20101 aacgactagt gtagatgagg taaagaaatc aactgatact gcttttaaac agacagtaga
    20161 cgttccggct cattatgtta atgcagcaaa agggaatggc ccatttctag caggtgtaaa
    20221 tcaaacgatt ccatatgaag cgtttggtgg aga
//
LOCUS       DATRQA010000023        20249 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.023, whole genome shotgun sequence.
ACCESSION   DATRQA010000023 DATRQA010000000
VERSION     DATRQA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20249
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.023"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            1..2262
                     /locus_tag="VBD27_000603"
     CDS             1..2262
                     /locus_tag="VBD27_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7024635.1"
                     /translation="MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPL
                     FSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDLPNTPPTQAGGNRGGGNGNGRNN
                     NRNQTATPPQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
                     IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKL
                     MEEIRQRQDVILFIDEIHEIVGAGTAGEGSMDAGNILKPALARGELQLVGATTLNEYR
                     IIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKYNNDAIEAAAVLSNR
                     YIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKAQATREEDYERAA
                     YFRDQIAKYKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLAD
                     DLKQHVIGQDDAVIKIAKAIRRNRVGLGSPNRPIGSFLFVGPTGVGKTELSKQLAIEL
                     FGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEIE
                     KAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGR
                     TNSVLGQLGNFFSPEFMNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDV
                     TDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYYLENPSEKELRAIMTSNGNII
                     IKSSKKTEESTKG"
     gene            2448..2903
                     /locus_tag="VBD27_000604"
     CDS             2448..2903
                     /locus_tag="VBD27_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7024636.1"
                     /translation="MTNPTFGEKIDNVDYRSRFGVYAIIPNPTHDKIILVQAPNGAWF
                     LPGGEIEENENHLEALTRELIEELGYSATIGHYYGQADEYFYSRHRDTYYYNPAYIYE
                     VTAYHKEQAPLEDFNHLAWFPIQEAKEKLKRGSHRWGVQAWEKNHHSRK"
     gene            2967..3269
                     /locus_tag="VBD27_000605"
     CDS             2967..3269
                     /locus_tag="VBD27_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO7024637.1"
                     /translation="MRLINTTSSHPELVRNQLQNTDAKLVEVYSAGNTDVVFTTAPKH
                     YELLISNKYRAIKDEELEAIREFFLKRKIDQSIIIQEQMKSLHTAKLIEISYPTTA"
     gene            complement(3339..6131)
                     /locus_tag="VBD27_000606"
     CDS             complement(3339..6131)
                     /locus_tag="VBD27_000606"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO7024638.1"
                     /translation="MKLKETLNLGQTAFPMRAGLPNKEPQWQEAWDQADIYKKRQALN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKKGVKRKEMDLAEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTPDYE
                     ADQVRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTVTASRGLTVGPDMEYVVVAPVGSERKYLLAEVLVDSLAAK
                     FGWENFEIVTHHTGKELNHIVTEHPWDTEVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLDVVVTVDSRGLMMENAGPDFEGQFYDKVTPLVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKTNFQPEWGKKRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFAEYGSIVWWQRDAKDLLPAGY
                     THPGSPNGLFEKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGEKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILKQTSETYRKIRNTLRFLIANTSDFNPKQDAVAYENLGAVDRYMT
                     IKFNQVVDTINKAYAAYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSPERRR
                     MQTVFYDILVKLTKLLTPILPHTAEEIWSYLEHEEEEFVQLAEMPVAQTFSGQEEILE
                     EWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASQEVKTLLTALNSDIALLMIV
                     SQLTIADEADKPADSVSFEGVAFTVEHAEGEVCERSRRIDPTTRMRSYGVAVCDASAA
                     IIEQYYPEAVAQGFEA"
     gene            complement(6416..7186)
                     /locus_tag="VBD27_000607"
     CDS             complement(6416..7186)
                     /locus_tag="VBD27_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEO7024639.1"
                     /translation="MPLTALEIKDKTFSSKFRGYSEEEVNEFLEIVVDDYEDLIRRNR
                     EQEQYIKDLEEKIAYFNEMKESLSQSVILAQETAERVKISAQDEASNLMGKATFDAQH
                     LIDEAKLKANQILRDATDDAKRVAIETEDLKRQSRVFHQRLLSELEGQLKLANSSAWE
                     ELLKPTAIYLQNSDASFKEVVEKVLDEDDALPVVDDTESFDATRQFSPDEMEELQRRV
                     EESNKQLEESGLLDTNNFQMEEPINLGETQTFKLNIED"
     gene            complement(7196..7984)
                     /locus_tag="VBD27_000608"
     CDS             complement(7196..7984)
                     /locus_tag="VBD27_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO7024640.1"
                     /translation="MTLDDIYQHFRPEEYAFINKIDHLAQYVENTYSFITTEFLNPRE
                     FKILESVLERRGSHYYTSGQYFQTEYVKVIIAPEYYQLDMVDFNLSLIEIKYNAKFNH
                     LTHAKIMGTLLNYLGVKRSILGDILVEEGCAQVLVDSQMTNHLVHSVTKIGTASVQLA
                     EVPLSKLLTPKQDIQKLTVIASSLRLDKILATILKISRTQSTKLIEADKVKVNYAIVN
                     RVSEQLVEGDLISVRGYGRFTLNHNLGLTKNQKYKLEVDKMIHN"
     gene            complement(7986..8240)
                     /locus_tag="VBD27_000609"
     CDS             complement(7986..8240)
                     /locus_tag="VBD27_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO7024641.1"
                     /translation="MILVLLILLKTIRVYSYILLAYALLSWLPGAYDSWIGRLVVQIV
                     DPIIRPLRRFNLQFLGLDFTVIVAFFLLNMLSRFLILLFT"
     gene            complement(8243..8848)
                     /locus_tag="VBD27_000610"
     CDS             complement(8243..8848)
                     /locus_tag="VBD27_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEO7024642.1"
                     /translation="MALKDRFDKIISYFDTDDVSENEVHEVQERTSVQRDSRAATAQE
                     ASQRSHMTNSAEEEMIGSRPRTSTYNPNRQERQRVQRDNAYQQATPRIQNKDSVRQQR
                     EQVTIALKYPRKYEDAQEIVDLLIVNECVLIDFQYMLDAQARRCLDYIDGASRVLYGS
                     LQKVGSSMFLLTPANVMVDIEEMNIPKTGQETSFDFDMKRR"
     gene            complement(8860..9534)
                     /locus_tag="VBD27_000611"
     CDS             complement(8860..9534)
                     /locus_tag="VBD27_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO7024643.1"
                     /translation="MMNLQENKTAIFDNVSKLALKAGRAPESVHIVAVTKYVNCQTTE
                     ALIRTGVNHIGENRVDKFLEKYQALKDEKLTWHLIGSLQRRKVKDVINYVDYFHALDS
                     VKLAAEIQKRAQKPVKCFLQVNISREDSKHGFTIEQIDDALNLISRYDKIELIGIMTM
                     APLKATKEEISSIFEETESLRKRLQARNIERMPFTELSMGMSRDYDIAIQNGSTFVRI
                     GTSFFK"
     gene            complement(9540..10820)
                     /gene="ftsZ"
                     /locus_tag="VBD27_000612"
     CDS             complement(9540..10820)
                     /gene="ftsZ"
                     /locus_tag="VBD27_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO7024644.1"
                     /translation="MVFSFDTASVQGAVIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEVLTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVITRPFGFEGNKRSNFAIEGIQELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERITEAARKAIYSPLLETTIDGAEDVIVNVTGGMDMTLTEA
                     EEASEIVSQAAGKGVNIWLGTSIDMDMKDEIRVTVVATGVRKDKTNQVSGFTTSAPTN
                     QAPSERQSTSNSNFDRRGNFDMTESREMPTQQNQPHAQNQQQSSAFGNWDLRRDNISR
                     PTEGELDSKLSMSTFSENDDMDDELETPPFFKNR"
     gene            complement(10842..12215)
                     /gene="ftsA"
                     /locus_tag="VBD27_000613"
     CDS             complement(10842..12215)
                     /gene="ftsA"
                     /locus_tag="VBD27_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO7024645.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFIANEMNVIGVSNVPSSGVKD
                     GIIIDIEAAATAIKEAVKQAEEKAGITIDKINVGLPANLLQIEPTQGMIPVPNESKEI
                     KDEDVESVVKSALTKSITPEREVISLIPLEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPTTILHNLRKTVERAGIKVEHVVIAPLALAKSVLNEGEREFGATVIDMGGGQTTVAS
                     MRNQELQYTNIYSEGSDYVTKDISKVLRTTVEIAEALKFNFGQANVEEASTSDTVQVN
                     VVGNEEPVEITESYLSQIISGRIRQILEHVKQDLGRGRLLDLPGGIILVGGGAIMPGV
                     VEVAQQIFGTRVKLHVPNQVGIRNPMFANVISIVDYVGMMSEVDIIAQHAVTGDEMLR
                     HKPVDFDYKEKTNTMSTMPYSEPLTSSMEDSNLEPIRARENAQEPTEPKTNIGERIRG
                     IFGSMFD"
     gene            complement(12488..13624)
                     /locus_tag="VBD27_000614"
     CDS             complement(12488..13624)
                     /locus_tag="VBD27_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEO7024646.1"
                     /translation="MPKKKSDTPEKEEVVLTEWQKRNLEFLKKRKEDEEEQKRINEKL
                     RLDKRSKLNISSPEEPQNTTKIKKLHFPKISKPKIEKKQKKEKIVNSLAKTNRIRTAP
                     IFVVAFLVILVSVFLLTPFSKQKTITVSGNQHTPDDILIEKTNIQKNDYFFSLIFKHK
                     AIEQRLAAEDVWVKTAQMTYQFPNKFHIQVQENKIIAYAHTKQGYQPVLETGKKADPV
                     NSSELPKHFLTINLDKEDSIKLLIKDLKALDPDLISEIQVISLADSKTTPDLLLLDMH
                     DGNSIRIPLSKFKERLPFYKQIKKNLKEPSIVDMEVGVYTTTSTIESTPVKAEDTKNK
                     STDKTQTQNGQVAENSQGQTNNSNTNQQGQQIATEQAPNPQNVN"
     gene            complement(13628..14704)
                     /locus_tag="VBD27_000615"
     CDS             complement(13628..14704)
                     /locus_tag="VBD27_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEO7024647.1"
                     /translation="MGKKIVFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     EQINQSGLDITFHSIATGKLRRYFSWQNMLDVFKVGVGVLQSIAIIAKLRPQALFSKG
                     GFVSVPPVVAARLLKVPVFVHESDLSMGLANKIAYKFATIMYTTFEQSKDLIKTKHIG
                     AVTKVMDCKKSFENTDLTSIKEAFDPNLKTLLFIGGSAGAKVFNDFITQTPELEEKYN
                     VINISGDSSLNRLKKNLYRVDYVTDLYQPLMNLADVVVTRGGSNTIFELVAMKKLHLI
                     IPLGREASRGDQLENAAYFEEKGYALQLPESELNINTLEKQINLLISNSESYEKNMSQ
                     SSEIKSQDEFYQLLIDDMAKVTKG"
     gene            complement(14707..16062)
                     /locus_tag="VBD27_000616"
     CDS             complement(14707..16062)
                     /locus_tag="VBD27_000616"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO7024648.1"
                     /translation="MKTITTFENKKVLVLGLARSGEAAARLLAKLGAIVTVNDGKPFD
                     ENPTAQSLLEEGIKVVCGSHPLELLDEDFCYMIKNPGIPYNNPMVKKALEKQIPVLTE
                     VELAYLVSESQLIGITGSNGKTTTTTMIAEVLNAGGQRGLLAGNIGFPASEVVQAADD
                     KDILVMELSSFQLMGVKEFRPHIAVITNLMPTHLDYHGSFEDYVAAKWNIQNQMSSSD
                     FLVLNFNQGISKELAKTTKATIVPFSTTEKVDGAYVQDKQLFYKGENIMSVDDIGVPG
                     SHNVENALATIAVAKLAGISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNI
                     LATQKALSGFDNTKVILIAGGLDRGNEFDELIPDITGLKHMVVLGESASRVKRAAQKA
                     GVTYSDALDVRDAVHKAYEVAQQGDVILLSPANASWDMYKNFEVRGDEFIDTFESLRG
                     E"
     gene            complement(16192..16437)
                     /locus_tag="VBD27_000617"
     CDS             complement(16192..16437)
                     /locus_tag="VBD27_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEO7024649.1"
                     /translation="MFYLIVALLIALYYFFMAPKTVRNTLNAIGLVGLVALLLVLAVM
                     SFIKILQLPGELYIGLIMIPLGYTAFKETLNLSEKKK"
     gene            complement(16482..18323)
                     /gene="typA"
                     /locus_tag="VBD27_000618"
     CDS             complement(16482..18323)
                     /gene="typA"
                     /locus_tag="VBD27_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO7024650.1"
                     /translation="MTNLRTDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELEER
                     AMDSNDIEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPSEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSMSDDPSDQEKTMAPIFDTIIDHIPAPVDNSEEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAIEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVQCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGVLVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLADDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            complement(18555..18935)
                     /locus_tag="VBD27_000619"
     CDS             complement(18555..18935)
                     /locus_tag="VBD27_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO7024651.1"
                     /translation="MDMSVILIIVILLAFVAWASWNYWRVRRAAKFLDNESFQKEMSR
                     GQLIDIREAGAFHRKHILGARNIPASQFKVALSALRKDKPVLLYDASRGQSIPRIVLL
                     LRKEGFNQLYVLKDGFNYWTGRVK"
     gene            complement(18947..19915)
                     /locus_tag="VBD27_000620"
     CDS             complement(18947..19915)
                     /locus_tag="VBD27_000620"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEO7024652.1"
                     /translation="MSKKLLGIDLGGTTIKFGILTLEGEVQEKWAIETNTLENGRHIV
                     SDIVESLKHRLSLYGLTKDDFLGIGMGSPGAVDRTSKTVTGAFNLNWADTQEVGSVIE
                     KEVGIPFFIDNDANVAALGERWVGAGANNPDVVFVTLGTGVGGGVIADGNLIHGVAGA
                     GGEIGHMIVDPENGFTCTCGNKGCLETVASATGVVRVARQLAEQYEGSSAIKAAIDNG
                     DTVTSKDIFIAAEDGDKFANSVVERVSRYLGLAAANISNILNPDSVVIGGGVSAAGEF
                     LRSRVEKYFVTFAFPQVKKSTKIKIAELGNDAGIIGAASLANQQAS"
     gene            complement(19912..20121)
                     /locus_tag="VBD27_000621"
     CDS             complement(19912..20121)
                     /locus_tag="VBD27_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEO7024653.1"
                     /translation="MKVLFDVQNLLKKFGIYVYIGKRLYDIEVMKIELQRLYDNGLIS
                     RDDYLKAELILRREHRLELEKENKK"
BASE COUNT         6293 a         4044 c         3288 g         6624 t
ORIGIN      
        1 atgctctgtc aaaactgcaa actaaacgaa tcaactattc atttgtatac aaatgttaat
       61 ggaaaacaaa agcaagttga tctttgtcaa aattgctacc aaattattaa aactgaccct
      121 aataatccac ttttttcagg tttaaatcat gtttcacatg cgcctggtgg catcaatcct
      181 ttctttgatg atttctttgg agatttaaat aatttcagag cctttaatgg ccaagattta
      241 ccaaacactc ctcctacaca agcaggagga aaccgaggtg gaggaaacgg taatggacga
      301 aataataatc gcaaccaaac tgcaactcct cctcaagcca aagggattct agaagaattt
      361 ggtatcaatg tcactgaaat agcccgccat ggtgatattg accctgttat tggacgtgac
      421 tcagaaatta ttcgtgttat cgaaatcctt aatcgtcgta caaaaaataa tcctgtactt
      481 attggcgaac ccggcgtagg gaaaactgcc gttgttgaag gacttgctca gaaaattgtt
      541 gatggtaatg tgcctcataa acttcaaggc aaacaagtta ttcgtttgga tgttgtgagt
      601 ctagtacaag gaacgggcat tcgtggtcaa tttgaagaac gcatgcaaaa gttgatggaa
      661 gaaattcgtc aacgtcaaga tgttattcta ttcatagatg aaattcacga aatagttgga
      721 gctggtacag ctggcgaagg tagtatggat gcaggtaata ttttaaaacc tgcccttgca
      781 cgtggagaat tacaactagt aggtgccact actctcaatg aatatcgtat tatcgaaaag
      841 gacgctgcct tagaacgccg tatgcaacct gtaaaagtcg atgaaccttc tgttgaagaa
      901 accattacta ttttgaaagg tatccaaaaa aaatacgaag attatcatca cgtaaaatat
      961 aataatgatg ccatagaagc agctgcagta ctatctaatc gttatatcca agaccgcttt
     1021 ttacctgata aagcaataga cttattagat gaagctggtt ctaaaatgaa cctaacacta
     1081 aattttgttg atccaaaaga aattgatcaa cgtctcattg aagcagaaaa tttaaaagcg
     1141 caagcgactc gtgaagaaga ttacgaacgt gcagcttact tccgtgacca gattgcaaaa
     1201 tataaagaaa tgcagcaaca aaaggtcgac gatcaagata cacctattat taccgaaaaa
     1261 acaattgagc acatcattga agaaaaaacg aatatccctg ttggtgattt aaaagaaaaa
     1321 gaacaatctc aattaattaa tctcgcagat gacttgaaac agcatgtgat cggccaggat
     1381 gacgctgtca ttaagattgc aaaagctatt cgtcgtaatc gagttggtct tggtagccca
     1441 aaccgtccta ttggttcctt tttatttgta ggaccaaccg gtgttggtaa aactgaactt
     1501 tctaaacaac tagcaattga gctctttggt tcagctgata gtatgattcg ttttgatatg
     1561 tcagagtaca tggaaaagca tgctgttgct aaattagtcg gagcgcctcc aggatacgtg
     1621 ggatacgagg aagctggaca actaactgaa aaggttcgtc gaaatcctta ctcgctcatc
     1681 cttctagatg aaattgaaaa agctcatccc gatgtcatgc atatgttctt gcaggtcctt
     1741 gatgacggtc gattaacaga tggacaagga agaactgtta gttttaaaga taccattatc
     1801 atcatgacct caaatgctgg ttctggtaaa actgaagcaa gtgttggctt tggtgcctca
     1861 cgagaaggta ggacgaattc cgtactaggt caactaggta acttcttcag ccctgaattt
     1921 atgaaccgct ttgacggtat tattgaattc aaggctttag ataaagagaa tctcctcaat
     1981 atcgttgata ttatgttatc tgacgttaat gcacgtctcg ccatcaatgg tattcatcta
     2041 gatgtcactg ataaagtgaa agaaaaattg gttgatttag gttacgatcc caaaatggga
     2101 gcacgtccat tacgtcgtac cattcaagaa catattgaag atgctatcac agattactat
     2161 cttgagaatc caagcgaaaa agaacttcgt gctattatga ctagcaatgg aaatatcata
     2221 ataaaatctt ctaaaaaaac tgaggaaagt acaaaaggtt gagtaagaaa ctcaaccttt
     2281 tttcacactc ttctcaaaaa ataatagcct ctataaagct gtttctctcc tatctcgtct
     2341 ttaaatttta atacaattat taaggaaata ttccaaaaat tttagtataa taaataaaaa
     2401 ataattaaag cttttaaaat cataaaacac aatcaggaga gagaactatg actaatccta
     2461 ctttcggtga aaaaatagat aatgtagact ataggtcacg atttggtgtc tatgctatta
     2521 ttccaaatcc aactcatgat aaaattattt tagtacaggc acctaatggt gcttggtttc
     2581 ttccaggtgg ggaaattgaa gaaaatgaaa atcacctaga agctttaact cgtgagttaa
     2641 ttgaagaatt aggttattct gcaacaattg gccattacta tggtcaagct gatgaatatt
     2701 tttactctag acaccgtgat acttactact ataatcccgc ctatatttat gaagttactg
     2761 cctatcataa ggagcaagcg ccactagaag atttcaacca tctagcctgg tttcctatcc
     2821 aagaagctaa agaaaagcta aaacgaggaa gccatagatg gggtgtccaa gcttgggaaa
     2881 aaaatcacca ttctaggaaa taattcatac ctttcttcct aaatagtgtt ataataaacc
     2941 taataagaat cagggggaaa atagttatga gactaattaa tacaaccagt agtcatcctg
     3001 aactagttcg aaatcaactc caaaatacag atgcaaaatt agtggaagtc tattcagctg
     3061 gcaacaccga tgtcgttttt acaacagcac ctaaacatta tgaattatta atttcaaaca
     3121 aatatcgtgc tatcaaagat gaagaattag aagctattcg tgagttcttc ttaaagcgta
     3181 aaatagatca atctattatt attcaagagc agatgaaatc actccatact gccaagttaa
     3241 ttgaaatttc atatccaaca acagcataat taaaagaagc ctaatcaagg cttctttttt
     3301 gatgactact aaacaaatat agcattaaat aatttttatt aagcttcaaa accttgagct
     3361 actgcttcag gataatattg ctcaataatt gctgcactag catcacaaac agctacacca
     3421 tacgaacgca tccttgtggt tggatcaata cggcgtgagc gttcgcagac ttcaccttca
     3481 gcgtgttcaa cagtaaatgc aacaccttca aatgatactg agtcagctgg tttgtctgct
     3541 tcatcagcaa ttgttaattg tgagacaatc atcaagagtg caatatcgct atttaacgct
     3601 gttaataacg ttttcacttc ttgactagca taaattgtta aatgtgcttc tagagattta
     3661 ccaatgactt tagcgttacg tgcttcttcg agagcttttt gagcttgtgt tcgtaacgtc
     3721 atgaatgctg accactcttc aagaatttct tcttgaccag agaatgtctg tgcaacaggc
     3781 atttctgcta attgaacaaa ttcctcctcc tcatgttcaa ggtatgacca aatttcttca
     3841 gcagtatgtg gtagaatcgg agtcaaaagt tttgtaagtt taactaaaat atcgtagaaa
     3901 acagtttgca tacgacgtcg ttctggactg ttagccgctt caatgtaaac cacatctttt
     3961 gcaaaatcaa ggtaaaatgc tgatagatca actgttacga agttaacgac agctttataa
     4021 attgccataa aatcataagc agcataagct ttgttgattg tatccacaac ttgattaaat
     4081 ttaatagtca tataacgatc aacggcacct aaattttcat aggctactgc atcttgttta
     4141 ggattaaaat cggaagtatt tgcaatcaag aaacgcaatg tatttctgat cttacgataa
     4201 gtttctgatg tttgttttaa gatatccatt gaaatacgaa catcattact tgaatcaaca
     4261 gaagttaccc acagacgcaa gatttccgca ccaaattgtt tttcaacatc actcggaaga
     4321 attgtatttc ctagagattt agacattttc tcacctttac catcaagtac aaaaccttga
     4381 gataagactg ctttgtatgg agcgtgacca ttaacagcaa ctgatgtaat tagtgatgag
     4441 ttgaaccaac cacggtattg atcagaacct tcaagataaa gatctgctgg ataggaaagg
     4501 ttttcacgag cattcattac tccattccat gatgaaccag aatcaaacca aacatccatg
     4561 atgtctgttt ctttctcaaa gagaccatta ggtgaacctg gatgagtata acctgctggt
     4621 agaaggtcct tagcatcacg ttgccaccaa acgattgagc cgtattcagc aaataaatct
     4681 gcaacgtgat cagtcacttc ttttgtcatg atagctgtac catcttctgc ataaaaaatt
     4741 ggaagtggaa caccccaagc acgttgacgt gagattaccc agtcgccacg gtcacggatc
     4801 atgttataaa gacgtttttt accccactca ggttggaaat ttgtcttttc aatttcatcg
     4861 aggatttctt gacggaattt cgaaacagag gcaaaccatt gtggaaccgc acgccaaatg
     4921 attggctttt tcgtacgcca gtcaaatgga tatgagtggt tgataacttc gctggctaaa
     4981 agaagatctc ccaatttttc ttttacaagt ggtgtcactt tgtcatagaa ttgaccttca
     5041 aaatcaggac cagcattttc catcatcaaa ccacgactat ctacggtaac tacaacatca
     5101 agtccattag caataccaac gttatagtca tcttcaccaa aaccaggagc cgtgtggaca
     5161 atacctgtac cagaatctgt tgtaacatgg tctccaagga taaccaactc ttctacttct
     5221 gtatcccatg gatgttctgt aacaatgtga ttaagttctt taccagtgtg atgagtcaca
     5281 atttcaaagt tttcccagcc aaacttagca gcgagactat ctacaagaac ctctgcaaga
     5341 aggtatttac gctcactacc tactggtgca actacaacat actccatatc tggtcctact
     5401 gttaaaccgc gtgaagctgt tacagtaaat ggtgttgtcg tccaaacgac gatataggta
     5461 tctgtatcaa gaattccctt accatcttta actttattgg catagtagag tgatgtcgaa
     5521 tcaatatcat gatattcgat ttcagcctca gcaagggcag actctgatga ccatgaccaa
     5581 tacactggtt tagcaccacg atagatatat cctttatctg ccatagcacc gaaaacacgt
     5641 acttgatctg cttcataatc tggtgtcagt gtaatataag gattttccca atcagcagaa
     5701 acgcccagac gtttaaaatc atcacgttgt ttgtcaactt ggctgagagc ataatcacga
     5761 cacatttcaa ggtattcagc caagtccatt tctttacgtt taaccccttt tttagctaat
     5821 acttgctcaa ttgggagtcc atgagtatcc caaccaggca cataaggagc tcgaaaacct
     5881 gacattgatt ttgaacgtac aataatgtct ttagaaattt tatttagtgc atgtcctaca
     5941 tgaatattcc cattagcata gggaggtccg tcatgaaggt gaaaggctgg ttttccttca
     6001 ttcaatgctt gacgtttttt ataaatatca gcttgatccc atgcttcttg ccattgaggt
     6061 tccttatttg gaagccctgc acgcattgga aaagctgttt gtcctaaatt caaggtttct
     6121 tttaatttca tactaactcc ttatctcata gtaatagtta tcacaatttt ttaaattgct
     6181 aaataaaaaa gctttcatca cataaaggac gaaagctcgt ggtaccacct taattcagag
     6241 cagaaaatag ctatttctta ctccctcaac aacccgtaag gtgggttaag ccttctacct
     6301 tacttaaata atagttcagg tagaatgatt gaaaatgata atttcaatat tagggattgt
     6361 agggctctca ccatctccta ctcgctgata atataatatt aaaaccttgt ttttcttaat
     6421 cttcaatatt taatttaaaa gtttgagttt cacctaaatt gatcggttct tccatttgga
     6481 aattattagt atctaataaa cctgactctt caagttgttt gttactttct tcaacacgac
     6541 gctgtaattc ttccatttca tctggtgaga attgacgagt agcatcaaat gactcagtgt
     6601 catccaccac tggtaaagca tcatcttcat caagaacttt ttcaacaact tctttgaaag
     6661 aagcatcaga attttgaaga taaatagctg ttggtttcaa caattcttcc caagcagatg
     6721 agttagctaa ttttaactga ccttctaatt cagaaagtaa gcgttggtgg aaaacacgtg
     6781 attgacgttt caaatcttct gtttctatag cgacacgctt agcatcatct gtcgcatcac
     6841 gaaggatttg atttgctttt aatttagcct catcaattaa atgttgagca tcaaatgtag
     6901 ctttccccat taggttagat gcttcatcct gtgctgaaat tttcacacgt tcagcagttt
     6961 cttgagctaa aataactgat tgacttaacg attccttcat ttcgttgaag taagcgattt
     7021 tctcttctaa atctttaatg tattgctctt gctcacgatt acgtctaatc aagtcttcgt
     7081 aatcgtcaac aacaatctct aaaaattcat taacttcttc ttcgctataa ccgcgaaatt
     7141 ttgatgaaaa tgttttatct ttaatttcaa gtgctgtaag tggcatgttt tctccttagt
     7201 tatgtatcat tttgtctact tctaacttat atttttgatt tttagttaac cctaggttat
     7261 ggttcaacgt aaatcgacca tatcctctaa cactaatcaa atccccctct actaattgtt
     7321 cagaaactcg attgacgatt gcataattga ccttgacttt atctgcctca atcaatttcg
     7381 tcgactgtgt tcgtgaaatc tttaaaatcg ttgctaaaat tttatcaaga cgtaaactgg
     7441 aagcaataac agttagtttt tgaatatctt gtttgggagt tagaagtttg gacaagggaa
     7501 cttcagctaa ttgtacacta gcagttccaa tttttgtaac tgaatgaaca aggtgatttg
     7561 tcatttgact atcgaccaaa acctgagcac agccttcttc aactaggata tcccctaaaa
     7621 ttgatcgttt tacacctaag taattcagta aagttcccat aattttagca tgggtaaggt
     7681 gattaaattt agcattatac tttatttcaa ttaggctaag gttaaaatca accatatcta
     7741 actgatagta ttccggtgct attattactt taacatattc tgtttgaaaa tactgaccag
     7801 aagtgtaata atgactacct cgcctctcta gaacactttc gagtatttta aattccctag
     7861 gatttagaaa ttcagtggta ataaacgaat aggtgttttc gacatattga gctaaatggt
     7921 ctattttatt gataaacgca tactcttctg gtcgaaaatg ctgataaata tcatctaacg
     7981 tcatcttatg taaaaagcaa gattaaaaat ctactcaaca tatttagtaa aaagaatgct
     8041 actattactg taaaatctag acctaaaaat tgtagattaa atcttctgag tgggcgaata
     8101 atcggatcaa caatctgtac cactagtcta ccaatccatg aatcgtatgc tcccggaagc
     8161 caagataaca aggcataggc tagtaagata taagaataga ccctaattgt ttttaataat
     8221 attaatagta ctaatatcat atttatcgtc tcttcatatc aaaatcaaaa ctcgtctctt
     8281 gaccagtctt tggaatattc atctcctcaa tatcgaccat aacattggct ggtgttaata
     8341 gaaacattga actcccaacc ttttgtaatg aaccatataa tactctactt gcaccatcaa
     8401 tataatccaa gcaacgtcgt gcttgcgcat caagcatata ttgaaaatca atcaagacac
     8461 attcattaac gataagtaaa tcaacaattt cttgtgcatc ctcatactta cggggatact
     8521 tcagagcaat tgttacttgt tctctctgtt gtcgaactga gtctttattt tgaatcctag
     8581 gagtagcttg ctgataagca ttgtctctct gtaccctttg tctctcctga cgattaggat
     8641 tataggtaga agttctcgga cgactaccaa tcatctcttc ctctgcggag ttagtcatat
     8701 gactacgctg agaagcttcc tgtgctgtag ctgctctgga atctctttgc actgaagttc
     8761 tctcttgtac ttcgtgtact tcattttcac ttacatcgtc agtgtcaaaa taagaaataa
     8821 ttttgtcaaa tctatctttt aatgccatat tcccctccat tatttaaaga atgaagttcc
     8881 tatccttaca aatgttgatc cattttgaat agcaatatca taatctctac tcatgcccat
     8941 gcttaattct gtaaacggca ttcgttctat gttcctagct tgaagccttt tcctaagact
     9001 ctcagtctct tcaaaaattg atgatatttc ctctttagtt gcttttaagg gagccatagt
     9061 catgatgccg ataagttcaa ttttatcgta ccgagaaatt aagttcagtg catcatctat
     9121 ttgctcaata gtaaaaccat gcttactgtc ttctcgtgat atattaactt gtagaaaaca
     9181 tttaactggt ttttgagcac gtttttgaat ctcagctgct aacttaacag aatctaaagc
     9241 atgaaaataa tcaacataat taatgacatc tttaactttt cgacgttgta aactaccaat
     9301 gagatgccat gtaagctttt catcttttaa tgcttgatat ttttcaagaa atttatcaac
     9361 acgattttca ccgatatgat taacacctgt tctaataagc gcttctgttg tttgacagtt
     9421 aacatatttt gttacagcta cgatatgaac tgattcagga gcgcgacctg cttttagtgc
     9481 taatttacta acattgtcaa aaatcgctgt tttattttct tgtaaattca tcattttaat
     9541 taacggtttt taaagaatgg aggtgtttca agttcatcat ccatatcatc attttctgaa
     9601 aacgttgaca tagacaactt gctatcaagt tcaccttctg tcggacgtga aatattatca
     9661 cgacgaaggt cccaattacc aaaagcagaa ctttgttgct gattttgtgc atgaggctga
     9721 ttttgttgag ttggcatttc gcgagactct gtcatatcaa agttgccacg acgatcaaag
     9781 tttgaatttg aagtactttg acgttctgaa ggtgcttgat tagttggagc tgatgttgta
     9841 aaaccagata cttgattagt tttatcttta cgcacacctg ttgcaacaac tgttacgcgg
     9901 atttcgtctt tcatatccat gtcaattgaa gtgcctaacc aaatattgac acctttacct
     9961 gctgcttggc taacaatctc tgatgcttcc tctgcttcag taagtgtcat atccataccg
    10021 ccggtaacat tgacaataac gtcttctgct ccatcaatcg ttgtttcaag aagtggtgaa
    10081 taaattgctt ttcgagcagc ttcagtaata cgttcctcac cagaaccgat accaattccc
    10141 atcagtgcat tacctttatt tgccataact gttttaacat ctgcaaagtc aaggttaatt
    10201 aaacctgggt tagtaatcaa atctgtaata ccttgaacac cttgacgtaa aacattatct
    10261 gcttcactaa gagcttcaag caatggtgtc ttcttatcta caatttcaag aagattatta
    10321 tttgaaatga taagcagtgt atcaacttgt tctcttaatt cttgaatacc ttcgatagcg
    10381 aagttagaac gtttattacc ctcaaagcca aaaggacgtg tgataaccgc tactgtaagt
    10441 gctcctaaac tctttgcaat acgtgcaata actggagccg cacctgtacc agatccgccc
    10501 cccatacctg ctgtgataaa taccatatca gcgccagtaa gtgcttctgt taaaacttct
    10561 tcactttctt cagctgcttt acgaccaact tcaggttgtc ctcctgcacc aagtccgcga
    10621 gttaattttg gaccaagttg aatgacagtt tcagcttttg agctacttaa ggcttgaata
    10681 tcagtatttg ctgcaataaa ctcaacgcca gcgacacctt cgtcaatcat gcggttaata
    10741 gcgttaccgc caccgcctcc aacaccaata actttaataa ctgcaccttg tactgatgct
    10801 gtatcaaatg aaaataccat ttttattcct cacttattgt tttagtcaaa catacttcca
    10861 aaaattcctc tgatgcgctc accaatatta gttttaggct cagtaggctc ttgagcgttt
    10921 tctcttgctc ggattggttc taaattagaa tcttccatag aagaggttaa cggctcagaa
    10981 taaggcattg tcgacattgt atttgttttt tctttataat caaagtcgac tggtttatgt
    11041 cttagcattt cgtcgccagt tacagcatgt tgtgcgataa tgtcaacttc tgacatcatt
    11101 cctacataat caacgatact aataacatta gcgaacattg gattgcgaat acctacttgg
    11161 tttggaacgt gcaatttaac tctagttcca aaaatttgtt gagcaacctc gacaactcct
    11221 ggcataattg caccacctcc aacaagaata ataccaccag gtaaatcaag taaacgacct
    11281 ctacccaagt cttgtttgac atgttctaaa atttgacgaa tacgtcctga aataatttgc
    11341 gaaagatagc tttctgtaat ttcaacaggt tcctcgttac caacaacatt aacttgaact
    11401 gtatcagaag tacttgcttc ttcaacatta gcttgtccaa agttgaattt caatgcttcg
    11461 gcaatttcta ctgttgtacg taacacttta gaaatatctt tggtgacata atcgctacct
    11521 tcagaataaa tattagtata ttgcaattct tgattacgca tagaagcgac tgttgtttgt
    11581 ccgcctccca tatcaatcac agttgcacca aactcacgtt caccttcgtt tagaactgat
    11641 tttgctaagg ctagaggggc aatcacaaca tgctctactt ttatacctgc tcgttcaaca
    11701 gttttacgaa gattatgaag gatagttgtt ggtccagtat agataagacc acgcatctca
    11761 aggcgaatcc ccatcatccc tctagggtct ctaatccctt ggaaaccatc tacaatgaat
    11821 tcaagcggaa tcaatgaaat aacttctctt tcaggagtaa tgcttttagt caatgctgat
    11881 ttaacaacac tttcgacatc ttcatcctta atttcttttg attcattggg aacaggaatc
    11941 attccttgag ttggttcaat ttggagaaga tttgctggta atccaacatt gattttgtca
    12001 atggtgatgc ctgctttctc ctcagcttgt tttaccgcct ctttgattgc agttgctgct
    12061 gcctcaatat caataattat accatctttt acaccggaac tagggacgtt gctaacccca
    12121 attacattca tttcatttgc aataaattct gcaactaaaa ctttaatcga gcttgttcct
    12181 atatccaaac cagtaaaaaa gccatttcta gccattcact cgacctcgct atctttccat
    12241 tcttttctat tctacatctg ccaaaaacag aattattcag aaaatattat accataaaaa
    12301 aagcagaatt gttaccaaat tacgtttttt tattaagcaa catcaaattg aaatatttct
    12361 aacttattta aaccattcgc aacttataac aaacttaaat ttttttcaag aaatcaattt
    12421 ttactagcaa tctatgactg acttgatatg tataaaaaag actgataaat ccagtctttt
    12481 tttatcgcta attaacattt tgagggttgg gtgcctgctc tgttgctatc tgttgtcctt
    12541 gttgattagt atttgagtta tttgtttgtc cttgactatt ttccgcaacc tgaccatttt
    12601 gtgtttgtgt tttatcagtt gatttatttt ttgtatcttc cgctttcaca ggggttgatt
    12661 caatagtact tgttgttgtg taaactccca cttccatatc aacaatagaa ggttccttaa
    12721 ggttcttctt aatttgtttg taaaaaggaa gtctttcttt aaatttagat aatggtattc
    12781 taatactatt tccatcatgc atatctaaca gcaggaggtc aggtgtcgtt ttagaatcag
    12841 ctaaacttat cacctgaatc tcacttatta aatcagggtc taaagccttt aaatctttaa
    12901 ttaatagctt aatactatct tccttatcaa ggttaattgt taagaagtgc tttggtagct
    12961 ctgaactatt tacaggatca gccttttttc cagtttccaa gacaggctga tatccttgct
    13021 ttgtatgtgc atatgcaata atcttatttt cttgaacttg aatatgaaac ttattgggaa
    13081 attgataagt catctgagct gtttttaccc atacatcttc tgcagctaaa cgttgttcaa
    13141 tagctttatg tttaaaaatt aaagaaaaga aataatcgtt tttttgaata ttcgtttttt
    13201 ctatcaaaat atcatcaggt gtatgctgat tgccactaac tgttattgtt ttttgcttac
    13261 taaaaggagt tagtaggaaa acggaaacta aaatgactag gaatgctact acaaatatag
    13321 gtgcagttct aatgcgatta gttttggcta agctgttgac tattttttct tttttctgtt
    13381 tcttttcaat cttaggtttt gaaatctttg gaaaatgaag cttcttaatt ttagtagtat
    13441 tttgaggttc ttcaggagaa gaaatattta atttacttct tttatctaag cgtaattttt
    13501 cgttaatacg tttttgttct tcttcatctt ctttgcgttt ttttaaaaat tcaaggttac
    13561 gcttttgcca ttccgttaag acaacttctt ctttttctgg ggtatctgat ttcttcttag
    13621 gcatgtttta tccttttgtt acttttgcca tatcatcaat taataattga taaaattcat
    13681 cttgagattt tatttcagat gattgtgaca tgtttttttc gtaactctca ctgttagaaa
    13741 ttaataaatt tatctgtttc tcaagtgtat ttatgtttaa ttcagattct ggtaactgca
    13801 atgcgtagcc cttttcttca aaataagcag cattctctaa ttggtcacct cggctcgctt
    13861 cacgtccaag agggataata agatgaagtt ttttcatagc tactaattca aaaatagtat
    13921 tagagccacc tcgtgtaaca accacatctg ctaaattcat taaaggctga taaaggtctg
    13981 taacataatc cacgcgataa aggttcttct tcaatcgatt gagagaagaa tcgcctgaaa
    14041 tattgataac attatatttt tcttccagct caggtgtttg agtaataaaa tcattgaata
    14101 cttttgcacc tgctgatcct ccaataaata gtaaggtctt taggtttgga tcgaaggctt
    14161 cttttatact agttaagtca gtattttcaa acgacttttt acaatccatg acttttgtta
    14221 cagcaccaat atgcttagtt ttgattaaat ctttagattg ttcaaaagtc gtatacataa
    14281 tagtcgcaaa tttataagca atcttatttg ctaaccccat tgataaatct gattcatgaa
    14341 caaaaacagg aacttttaat aggcgtgcag ctactacagg aggtactgaa acaaatcccc
    14401 cttttgaaaa taaggcttgg ggtctaagtt tagcaataat agcaatcgat tgaagaacgc
    14461 caacccctac tttaaagaca tctaacatat tttgccatga aaaatagcga cgtagtttcc
    14521 cagtagctat agagtgaaat gtgatatcaa gtccagactg attaatttgt tcatgttcta
    14581 tgccattttt atcaccaata taatgtactt cccaaccatc tttgataaat ttggggatta
    14641 atattaggtt tagtgttaca tgacctaccg taccaccacc tgtaaaaaca atttttttcc
    14701 ccatagttac tctcctctaa gactttcgaa agtatcaatg aattcatcac cacggacttc
    14761 gaaattctta tacatgtccc atgatgcatt tgcaggactt agcaagataa catcgccctg
    14821 ttgtgccacc tcataagctt tatgtaccgc atctctaaca tctaaagcat cgctataagt
    14881 tactcctgct ttttgtgcag cacgttttac tcgagatgcc gattccccta aaacaaccat
    14941 atgtttaagt ccagtgatat ctggtatcaa ttcatcaaac tcattaccgc gatcaagacc
    15001 tcctgcaatt aggataactt tagtattatc aaagccagat aatgcttttt gagttgccaa
    15061 tatattagtt gacttgctgt cgttatagaa actaatacca tgaaccttac cgagtgattg
    15121 caagcggtgt ttaacacctc caaaattgct taaagtttct ctaataactt gattactgat
    15181 accagctagt ttagcaaccg caatagttgc tagagcattc tctacgttat ggcttcctgg
    15241 gacaccaatg tcatctactg acataatatt ctccccttta tagaaaagtt gcttgtcttg
    15301 tacgtaagca ccatcaactt tttccgtagt agagaaagga acgattgttg ctttagtagt
    15361 tttagctaac tctttagaaa taccttgatt aaaattaagt accaaaaaat cagatgaaga
    15421 catttgattt tggatattcc attttgcagc aacataatct tcaaaagacc catgataatc
    15481 taaatgagtt ggcattaaat tagtaattac tgcaatatga ggacgaaatt ccttaactcc
    15541 cattagctga aaacttgata attccataac tagaatatct ttatcatccg cagcctgaac
    15601 aacttcacta gcaggaaagc cgatattccc agctaacaaa cctctctgac ctccagcatt
    15661 taagacttct gcaatcatcg ttgtcgttgt cgttttcccg ttagagcctg taatacctat
    15721 tagctgagat tctgaaacta agtatgctaa ttccacttca gtcaaaacag ggatttgttt
    15781 ttctaatgct tttttgacca taggattgtt ataaggtatt cctggatttt taatcatgta
    15841 acaaaaatcc tcatctaaca attctaaagg atgactacca caaaccactt taataccctc
    15901 ttccaacaaa gactgtgctg ttggattttc atcaaatggt ttgccatcat taactgtcac
    15961 tattgctcct aacttagcta acaaacgtgc agcggcttct ccagatcgtg ctaaaccaag
    16021 gactaaaact tttttatttt caaatgttgt tattgttttc attactcgtc ccattctatc
    16081 attattatcc ctattatttt acctttaatc agtggctttt tcaaggtaaa ggatatgtct
    16141 ctaatttctc aaaacaaaag aacttaggag acaaaatcct aagttcataa gctatttctt
    16201 cttttctgac aaattcaatg tttctttaaa tgctgtatat cctagaggaa tcattataag
    16261 cccaatatac aattcacctg gcaattgaag gattttaata aaactcataa ctgccaagac
    16321 aagtaataag gctaccaaac ctactaaacc aatagcgttc aaagtattac gtactgtttt
    16381 tggtgccata aaaaagtaat ataaggcaat caaaagggca acaattaagt aaaacataga
    16441 actcctttct tattaatcat aaataagaaa tactttggat attattcagc tgattttttc
    16501 tttttattag ctttgtcacg tgctgcttta tttaaaattt gtttacgaag acgaatagac
    16561 tcaggagtta cttccatgta ctcatcatct gctaaaaatt caagtgactc ttctaaagta
    16621 aggatacgag gcgtcttgat gactgcagtt tgatctttag ttgctgaacg gacatttgtc
    16681 atttgtttag cagttgtaat attgactccg agatcattat cacgagaatt ctcaccaaca
    16741 atcattcctt cataaacttc tgtacctgga tttacaaaga tagtcccacg ttcttcaata
    16801 cgcataattg aatatgtagt tgctttacca ttttcaatag aaaccaagac accacgatga
    16861 cgaccaccaa tttctccttg aacaaccggt agatactggt caaaagtatg attcatgata
    16921 ccatatccac gtgtcattga aagaaactct gttgaataac caatcaaacc acgtgcagga
    16981 atcaagaaaa tcaaacgcgt ttgaccatta ccaaccatct gcatatcaag catatcacct
    17041 ttacgctctg acaaactttg gataatagca ccctgatatt cttctggagt atcaatttga
    17101 acacgctcaa acggctcgca ttgaacacca tcaatttctt tgatgataac ttctggacgt
    17161 gatacttgaa gttcatatcc ctcacgacgc attgtttcaa tgaggataga taaatgtaat
    17221 tctccacgcc ctgaaaccgt ccatttatct ggcgaatctg ttgggtcaac acgcaaagaa
    17281 acgtctgttt gtaattctgc taaaagacgt tcttcaacct tacgtgacgt aatccattta
    17341 ccttcgcgac ctgcaaatgg tgaattattc accaagaaag tcatttgaag tgttggctcg
    17401 tcaatacgta aaactggtag tggttcaata gcatctgtcg gagttactgt ctcaccaacg
    17461 aagatatctt ccataccaga aacagcgatt aaatcacccg ctttagcctc ttggatttct
    17521 ttacgttcaa gtccaaagaa accaaaaagt tttgttacgc ggaagttttt agttgtacca
    17581 tcaagttttg aaagagtaac ttgatctcca actttgacag tcccgcggaa aacacgtcca
    17641 ataccaatac gtcctacaaa atcattgtaa tcaagaagag aaacttggaa ttgaagtggt
    17701 tcttccgagt tgtcaactgg ggctggaatg tgatcaatga tagtatcaaa aatcggtgcc
    17761 attgtttttt cttgatctga aggatcatct gacattgaag atgttccatt gatggctgaa
    17821 gcataaacaa cagggaaatc tagttggtca tcatcagcac cgagctcaat gaagagttca
    17881 agaacttcat caacaacctc tgatggacga gctgatggct tatcaatttt atttacaaca
    17941 acgattggaa ttaagttttg ttcaagagct ttcttcaaaa caaaacgtgt ttgtggcatt
    18001 gttccttcgt aagcatcaac gactaaaaca acaccatcaa ccattttcat aatacgctca
    18061 acttcaccac caaagtccgc gtgaccaggt gtgtccataa tattgatacg aacatcgttg
    18121 tatgctacgg ctgtattttt tgcaagaatg gtgataccac gttctttctc gatatcattt
    18181 gaatccattg cacgttcttc aagctcttta cgctcatcaa gagtatgtga ttgttttaat
    18241 aattcatcaa cgagagttgt tttaccgtgg tcaacgtggg caatgatcgc aacgttacgg
    18301 atatctgttc ttaaatttgt catgttttcc tctattttat aatgtaattc tgttaactta
    18361 tgaattataa cacaattttt cttaaaaaac agaatttaca caagtgtaaa tgttttcata
    18421 ttaactgcct atttgtgata ggaaaacttt tcatcatttc attatttatg tcatttattt
    18481 tctacttttt tcaatttaat tcatttctac aaaaagagaa gagagttatt aatagtaact
    18541 ctctccttag tatattactt aactctaccc gtccagtaat tgaaaccgtc ctttaacacg
    18601 taaagttgat taaaaccttc ttttctgagt aacaaaacaa tccttggaat agactgacca
    18661 cggcttgcgt catataagag gacaggttta tctttgcgta aagccgataa ggcaacttta
    18721 aattgacttg ctggaatgtt acgtgctccg agaatatgct ttctatgaaa agcaccagct
    18781 tctcggatat caattaactg tcctcgtgac atttcttttt gaaaagactc gttatctaaa
    18841 aatttagctg ccctacgaac cctccaataa ttccaagatg cccaagcaac aaaagccaga
    18901 agaataacga taattaaaat aacagacata tccatatctt atctccttaa cttgcttgtt
    18961 gattggctaa gcttgctgca ccaataatac cagcatcatt acctagttca gcaatcttaa
    19021 ttttagttga ctttttaact tgtgggaaag caaatgtgac aaagtatttc tcaacgcgac
    19081 tacgtaaaaa ttcacctgct gctgagacac cgccaccaat aaccacagaa tcagggttta
    19141 aaatatttga aatattagct gctgccagtc caaggtaacg tgatacacgt tcaacaacag
    19201 aattagcaaa tttatcccca tcttctgctg ctataaaaat atctttactt gtaacagtat
    19261 caccgttgtc aatcgctgct ttaatggcag acgaaccttc atattgttct gcgagttgac
    19321 gtgctactct aacaacacct gtcgctgatg caactgtctc aagacagcct ttgttaccac
    19381 atgtgcacgt aaatccattt tctggatcaa caatcatatg cccaatttct ccacctgctc
    19441 ctgcaacacc atggatgagg ttaccatctg cgataacacc tccacctact cctgttccga
    19501 gggttacgaa aacaacatcg ggattattgg caccagcacc tacccagcgt tcaccaagtg
    19561 ctgcaacatt agcatcgtta tcaataaaaa atggaattcc aacttctttt tcaataaccg
    19621 aacctacttc ttgagtatca gcccaattta gattaaaagc acctgttact gttttactag
    19681 ttctatcaac agctcctgga gaacccatac cgataccgag aaagtcatct tttgttaatc
    19741 catagaggct caaacgatgt ttgagagatt caacgatatc agaaacgata tgtcttccgt
    19801 tttctaaagt attggtctca attgcccatt tttcttgtac ttctccctca agcgtcaaga
    19861 taccaaattt aatggtcgtt cctccgaggt caattcccaa taatttctta ctcatttttt
    19921 attttccttc tccaattcta gtctgtgctc tcttcttaaa atcagttctg cttttaaata
    19981 gtcatctcta cttatcagac cattatcgta gagacgttgc aattcaatct tcataacttc
    20041 gatatcataa agacgtttac caatataaac ataaatacca aattttttca aaagattctg
    20101 cacatcgaat aaaactttca tatatgaatt ttaacagaaa accattcaat tttcaagaaa
    20161 atccgcaacc gcttgctaat tgttattatg acgttaggct gactatctat aatagaaatc
    20221 ataatctctt ttctaccatt tttcgaata
//
LOCUS       DATRQA010000024        19894 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.024, whole genome shotgun sequence.
ACCESSION   DATRQA010000024 DATRQA010000000
VERSION     DATRQA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19894
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.024"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..630
                     /locus_tag="VBD27_000622"
     CDS             <1..630
                     /locus_tag="VBD27_000622"
                     /inference="COORDINATES: protein motif:HMM:NF019884.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7024654.1"
                     /translation="IQLNQRQERIIDIIKQFQPITGELIAEKLGVTRAALRSDLAVLS
                     MLGIIDAKPKVGYFYLGQYHASIGTSHFEKMTVSEIMGIPLTVHQKDSVYDVIVHIFM
                     EDAGCAFILDDDDFLCGVVSRKDLLKTSIGGGDLSKMPIGMVMTRMPHVTTVLENESL
                     FAAADKLVSRKVDSLPVVRHDKQYPEKFKVIGKLSKTILASLFLEIRDY"
     gene            641..1471
                     /locus_tag="VBD27_000623"
     CDS             641..1471
                     /locus_tag="VBD27_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO7024655.1"
                     /translation="MEDQLTIFIISDSLGETAKAIAKACLSQFPGHDDWHFQRFSYIN
                     SQERLEQVFEEASQKTVFMMFSLVDVALASYAQKRCESEHYAYVDLLTNVIQGISRIS
                     GIDPLGEPGILRRLDNDYFKRVESIEFAVKYDDGRDPRGILQADLVIIGISRTSKTPL
                     SMFLADKNIKVINIPLVPEVPVPKELRMIDSRRIIGLTNSVDHLNQVRKVRLKSLGLS
                     STANYASLERILEETRYAEEVMKNLGCPIINVSDKAIEETATIILEILKTNGQVAKNL
                     "
     gene            1484..4129
                     /locus_tag="VBD27_000624"
     CDS             1484..4129
                     /locus_tag="VBD27_000624"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEO7024656.1"
                     /translation="METKFVYHFDEGCKEMKELLGGKGANLAEMTSIGLPVPQGFTIT
                     TQACNDYYDNACHIRESILSQIDQAMAQLEVEQNKQLGSVDDPLLVSVRSGSVFSMPG
                     MMDTVLNLGLNDRSVQGLVKKTEDERFAYDSYRRFIQMFADVVTGIPKYKFDTILDRL
                     KTDKCYQDDTELTGSDLKRLVEFYKELYQKEAGEKFPQDPKRQLLLAIEAVFKSWNNP
                     RAKIYRKLNDIPETLGTAVNIQAMVFGNMGNNSGTGVAFTRNPSTGAANLFGEYLINA
                     QGEDVVAGIRTPQSISKLAEQMPIIYQEFVSVTQKLEAHYRDMQDMEFTIENGNLYML
                     QTRSGKRTAKAAIKIAVDQVNEGLISKEEAILRIEPKQLDQLLHPSFDLKSLKKAIIL
                     TTGLPASPGAAYGKVYFHAEDVVKEMKKGNPVLLVRQETSPEDIEGMVSANGIITARG
                     GMTSHAAVVARGMGKPCVAGCSQLLVDEVRREISIGHQTIKEGEMLSIDGATGNVYIG
                     QVPMAETSVDRDFEIFMKWVDENRDMMVCSNADNPRDAQKALDFGAEGIGLCRTEHMF
                     FDDERIPVVREMILADEILSRRKALERLLSFQRDDFYQIFKVLKGKACTIRLLDPPLH
                     EFLPHDKESIESMARQMGISTLAIEKRIQTLEEFNPMLGHRGCRLAITYPEIYQMQVR
                     ALVQGAILAMKEGYEAKPEIMIPLVTAHEEISIIRDLIEETIVEESKSKKINLSFPIG
                     TMIETPRACMIADDIAKFADFFSFGTNDLTQMSFGFSRDDAGKFLGEYVDKGLLKKDP
                     FQVLDQKGIGRFIEQAVRLGKEVKPNLKIGICGEHGGEPSSIEFCYQQGLHYVSCSPF
                     RIPIAKLAAAQAKIKQSRNDITIDK"
     gene            4267..4569
                     /gene="gatC"
                     /locus_tag="VBD27_000625"
     CDS             4267..4569
                     /gene="gatC"
                     /locus_tag="VBD27_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO7024657.1"
                     /translation="MKISEEEVRHVAHLSKLRFSDQETKEFASSLSKIVDMIELLNEV
                     DTEGVPVTTTMADRKTVMREDIAQPGHNRDDLFKNVPQHQDYYIKVPAILEDGGDA"
     gene            4569..6035
                     /gene="gatA"
                     /locus_tag="VBD27_000626"
     CDS             4569..6035
                     /gene="gatA"
                     /locus_tag="VBD27_000626"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO7024658.1"
                     /translation="MSFNNQSIDQLHDFLVKKEISATELTKATLEDIHAREQAVGSFI
                     TISDEMAIAQAKEIDDKGIDADNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATAVEKLYAKDMIVIGKANMDEFAMGGSTETSYFKKTNNAWDHSKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPASFNGIVGMKPTYGRVSRFGLFAFGSSLDQIGPMSQTV
                     KENAQLLTVISGHDVRDSTSSERTVGDFTAKIGQDIQGMKIALPKEYLGEGIAQGVKE
                     TIIKAAKHLEKLGAVIEEVSLPHSKYGVAVYYIVASSEASSNLQRFDGIRYGYRTENY
                     KNLDDIYVNTRSEGFGDEVKRRIMLGTFSLSSGYYDAYYKKAGQVRSLIIQDFEKVFA
                     DYDLILGPTAPTTAFDLDSLNHDPVAMYLADILTIPVNLAGLPGISIPAGFDQGLPVG
                     MQLIGPKFSEETIYQVAAAFEATTDYHKQQPKIFGGEN"
     gene            6035..7477
                     /gene="gatB"
                     /locus_tag="VBD27_000627"
     CDS             6035..7477
                     /gene="gatB"
                     /locus_tag="VBD27_000627"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO7024659.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPSSAHFGQEQNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHQNMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGTRKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEEFGTKAELKNLNS
                     FNNVRKGLIHEEKRQAQVLRSGGQIQQETRRFDETTGETILMRVKEGSSDYRYFPEPD
                     LPLFDISDEWIDQVRLELPEFPQERRAKYVSSFGLSSYDASQLTATKATSDFFEKAVA
                     IGGDAKQVSNWLQGEVAQFLNSESKSIEEIGLTPENLVEMIGLIADGTISSKIAKKVF
                     VHLAKNGGSAEEFVKKAGLVQISDPEVLIPIIHQVFADNEAAVIDFKSGKRNADKAFT
                     GYLMKATKGQANPQVALKLLAQELAKLKEE"
     gene            7607..8521
                     /locus_tag="VBD27_000628"
     CDS             7607..8521
                     /locus_tag="VBD27_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEO7024660.1"
                     /translation="MTKKEKGTMMTLAAGLAWGISGISGQYLMSHGVHVNLLTSLRLL
                     ITGIFLLSLARSKQKEHLVAAWKQPKFLKQVLLFSIFGLVLNQYAFLRAIHLTNAGTA
                     TVLQYMAPILILSIVCILNRQRPTSFEIIAIAMAILGTYMIATHGKLGSLAITPKGLM
                     WGLGSAITYSIYILLPVKLIHEWGSTIVIGSGMFIGGILFSLVTKAWQYPLQINVMSI
                     LAYIGIIGIGTIFAYTFFLKGVSIVGAVKGSLLASVEPVSSVFLTVLVLGEIFYPIDL
                     LGMLFIFLAVTLISYKDLIALKKMKRVR"
     gene            8605..9165
                     /locus_tag="VBD27_000629"
     CDS             8605..9165
                     /locus_tag="VBD27_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEO7024661.1"
                     /translation="MNSKIYFLEMKLSIDDYRPTFVVEAVYDLRAEDLLRHGIRAVLV
                     DLDNTLIAWNNPDGTAEVRAWLDEMTTADISVVVVSNNNHARVERAVSRFGVDFVSRA
                     MKPFTRGINMAIERYGFDRDEVIMVGDQLMTDIRASYRAGIKSVLVKPIVKSDAWNTK
                     FNRLRERRVWKKIEENYGKIVYQKGI"
     gene            9165..10283
                     /gene="yqeH"
                     /locus_tag="VBD27_000630"
     CDS             9165..10283
                     /gene="yqeH"
                     /locus_tag="VBD27_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEO7024662.1"
                     /translation="MEELFCIGCGARIQTENKDAAGYTPRAALEKGLETGELYCQRCF
                     RLRHYNEITDVHITDDEFLKLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFVAGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPVDVILTSAQNHHAIKDLIDTIEKY
                     RHGRDVYVVGVTNVGKSTLINAIIREITGSRDVITTSRFPGTTLDKIEIPLDDGSYIF
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNSEQTLFLAGLARFDFISGQKQ
                     GFTAYFDNNLNLHRTKLVGADEFYTKHVGKLLTPPTGKEVSDFPKLVRHEFTIKDKMD
                     IVYSGLGWIRVKSEAENPVVVAAWAPEGVAVVLRKALI"
     gene            10376..10693
                     /gene="yhbY"
                     /locus_tag="VBD27_000631"
     CDS             10376..10693
                     /gene="yhbY"
                     /locus_tag="VBD27_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO7024663.1"
                     /translation="MLTSKQRAFLKSEAHSMKPIIQIGKNGLNDQIKTSVRNALDARE
                     LIKVTLLQNTDEDIHDVAEVLEDEIGCDTVLKIGRILILYKESARKENRKISVKVKAV
                     KSN"
     gene            10823..11455
                     /locus_tag="VBD27_000632"
     CDS             10823..11455
                     /locus_tag="VBD27_000632"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO7024664.1"
                     /translation="MALELLTPFTKVELEEKKRDTNRKQIGIMGGNFNPVHNAHLVVA
                     DQVRQQLCLDQVLLMPEFQPPHIDKKETIDEQHRLKMLELAIEGIDGLSIEPIEIERK
                     CISYTYDTMKLLIEKNPDVDYYFIIGADMVEYLPKWHRIDELVKMVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRQFIKSNRQPNYLLPREVLDYIRKEGLYK"
     gene            11452..12039
                     /gene="yqeK"
                     /locus_tag="VBD27_000633"
     CDS             11452..12039
                     /gene="yqeK"
                     /locus_tag="VBD27_000633"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEO7024665.1"
                     /translation="MTYKDYTGLDRTELLSKVRHMMSDKRFNHVLGVERAAIELAERY
                     GYDKEKAGLAALLHDYAKELSDDEFLRLIDKYQLDPDLKKWGNNIWHGLVGIYKIQED
                     LAIKDQDILAAIAKHTVGSAQMSTLDKIVYVADYIEHNRDFPGVEEARELAKVDLNKA
                     VAYETARTVAFLASKAQPIYPKTIETYNAYIPYLD"
     gene            12102..12623
                     /locus_tag="VBD27_000634"
     CDS             12102..12623
                     /locus_tag="VBD27_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO7024666.1"
                     /translation="MTCALLLIDIQQGIMDKKPKHLTNFAELLDDLLLSAKGSNCEVI
                     WIRHHDKELPQGSPQWEIWEQRHLVTHHKIIDKTYNSCFKDTHLHDYLQSKHISQLIM
                     MGLQTEYCFDTSVKVAFEYGYDIFIPQGGHLTFDTPTLSGDSIKKHYENIWHHRFATM
                     VAKDSLLKLINEE"
     gene            12625..12981
                     /gene="rsfS"
                     /locus_tag="VBD27_000635"
     CDS             12625..12981
                     /gene="rsfS"
                     /locus_tag="VBD27_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO7024667.1"
                     /translation="MTEKDLLQLVVKAADEKRAEDIVILDLQPVTSVADYFVIMSASN
                     SRQLEAIADNIREQVKGNGGDASHLEGDSKAGWVLLDLNSVVVHIFSEDERQHYNLEK
                     LWHEAPLLDAEVFMTE"
     gene            13047..13781
                     /locus_tag="VBD27_000636"
     CDS             13047..13781
                     /locus_tag="VBD27_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7024668.1"
                     /translation="MATYETFAAVYDAVMDDTLYDKWTDFSLRHFPKGKKKLLELACG
                     TGIQSVRFAQAGYAVTGLDLSGDMLKLAKKRATSAHQSIQFIEGNMLDLSNVGKYDLI
                     TCYSDSICYMQDEVEVGDVFIEVYKALEENGVFIFDVHSTYQTDKVFPGYSYHENADD
                     FAMVWDTYEDDAPHSIVHELTFFVQEEDGRFTRHDEVHEERTYDILTYDILLEQAGFK
                     DVKVYADFEDKKPTATSARWFFVAHK"
     gene            13804..14055
                     /locus_tag="VBD27_000637"
     CDS             13804..14055
                     /locus_tag="VBD27_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024669.1"
                     /translation="MIIFLYACLMIFGILYIIERRVSQKKSKVILELLMNPKLHLDLL
                     SALKPFEKETDRLIYLRKTYHLDIIDAVTLNNQLKEKEV"
     gene            14052..15161
                     /locus_tag="VBD27_000638"
     CDS             14052..15161
                     /locus_tag="VBD27_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEO7024670.1"
                     /translation="MTVTGIVAEFNPFHNGHKYLLEQAQGIKVIAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVIELPFLVSVQSADYFASGAVSILARLGVDNLCFGTEEMLD
                     YARIGDIYVNKKEEMEAFLKKQSDSLSYPQKMQAMWQEFAGITFSGQTPNHILGLAYT
                     KAASQNGIRLNPIQRQGAGYHSSEKTEIFASATSLRKHQSDRFFVEKGMPNSDLFLNS
                     PQVVWQDYFSLLKYQIMTHSDLTQIYQVNEEIANRIKSQIRYVETVDELVDKVATKRY
                     TKARIRRLLTYILINAVESPIPNAIHVLGFTQKGQQHLKSVKKSVDIVTRIGSQTWDS
                     LTQRADSVYQMGNANIAEQTWGRIPFYQPVSQSDL"
     gene            15254..15968
                     /locus_tag="VBD27_000639"
                     /pseudo
     CDS             15254..15968
                     /locus_tag="VBD27_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859560.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            16107..16511
                     /locus_tag="VBD27_000640"
     CDS             16107..16511
                     /locus_tag="VBD27_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO7024671.1"
                     /translation="MKGFHGNIEKLTLGNTNFRQVLYTAEHCQLVLMTLPVGGEIGSE
                     IHAENDQFFRFEAGHGKVVIDGNEYEVADGDAIIVPAGAEHNVINTSETEMLKLYTIY
                     SPAHHKDGIIRATREEAEENEEDFDGVTTETK"
     gene            complement(16776..17489)
                     /locus_tag="VBD27_000641"
     CDS             complement(16776..17489)
                     /locus_tag="VBD27_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7024672.1"
                     /translation="MTQFLGEFLGTFILVLLGDGVVAGNVLSKTKEEGTGWTAIVFGW
                     GIACTVAVYVSGLFSPAHLNPAVTLAMASIGVISWGQVIPFIIAQMLGAMVAATILWL
                     HYYPHWKETKDSGLILASFSTGPAIRHTPSNLLGEIIGTAILVITIMAIGPSKVAAGL
                     DPIIVGIVIFAVGFSLGPTTGYAINPARDLGPRLMHAILPIENKGNSDWSYAWIPVVG
                     PIIGGVLGAILYNIALTLV"
     gene            complement(17499..17873)
                     /locus_tag="VBD27_000642"
     CDS             complement(17499..17873)
                     /locus_tag="VBD27_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEO7024673.1"
                     /translation="MSDIGIIVVSHSKNIAQGVVDLISEVAKDVSITYVGGTEDGEIG
                     TSFDQVQQIVEQNDKKTLLAFFDLGSAKMNLELVADFSEKNIIINSVPVVEGAYTAAA
                     LLQAGADLDSIQSQLAELTINK"
     gene            complement(17873..18451)
                     /gene="dhaL"
                     /locus_tag="VBD27_000643"
     CDS             complement(17873..18451)
                     /gene="dhaL"
                     /locus_tag="VBD27_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEO7024674.1"
                     /translation="MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGM
                     TAVIENLDNNEFSSAADVFKTVSMQLLSKVGGASGPLYGSAFMGITKAEQSESTISEA
                     LGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGMKATKGR
                     ASYVGERSVGHIDPGSFSSGLLFKALLEVGGV"
     gene            complement(18489..19478)
                     /gene="dhaK"
                     /locus_tag="VBD27_000644"
     CDS             complement(18489..19478)
                     /gene="dhaK"
                     /locus_tag="VBD27_000644"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEO7024675.1"
                     /translation="MKKILNQPTDVVTEMLDGLAYVHNDLVHRIEGFDIIARNEEKSG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAVCGAVFTSPTPDQVLEAIKEADEGAGVFMV
                     IKNYSGDIMNFEMAQDMAEMEGIEVASVVVDDDIAVEDSLYTQGKRGVAGTILVHKIL
                     GHAARHGKSLQEIKAIADELVPNIHTVGLALSGATVPEVGKPGFVLAEDEIEFGIGIH
                     GEPGYRKEKMQPSKALATELVDKLIESFDAKSGEKYGVLINGMGATPLMEQYVFANDV
                     AKLLEDKGIEVNYKKLGNYMTSIDMAGLSLTLIKLENQEWLEALNSDVTTIAW"
     gene            19652..>19894
                     /gene="dhaS"
                     /locus_tag="VBD27_000645"
     CDS             19652..>19894
                     /gene="dhaS"
                     /locus_tag="VBD27_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO7024676.1"
                     /translation="MTSSLITKKKIAKSFKRLFISQAFDKISVSDIMEDAGIRRQTFY
                     NHFVDKYALLEWIFQTELSEQVTDNLDYISGFQLLSE"
BASE COUNT         6550 a         3174 c         4013 g         6157 t
ORIGIN      
        1 attcaattaa atcaaaggca agaaagaatt attgatatta tcaaacagtt tcagcccatt
       61 actggtgagc taatagctga gaaattagga gtaacaagag cagcactaag gtctgatttg
      121 gcggttttaa gtatgctagg tatcatagat gcaaaaccta aggttggtta tttttattta
      181 ggacagtatc atgcttcaat agggacaagt cattttgaaa agatgacagt ttcagaaatt
      241 atggggatcc ctctgacagt tcatcaaaaa gattcagttt atgatgttat tgtacatatt
      301 tttatggaag atgctggttg tgcttttatc ttggatgatg atgattttct ctgtggagtc
      361 gtgtcacgta aagatttact aaaaaccagt attggcggag gagatctttc taaaatgcca
      421 ataggaatgg tgatgacacg tatgccacac gtgacaactg ttttagaaaa tgaaagtctt
      481 tttgcggcag ctgataaatt agtgagcaga aaagtggata gtctccctgt cgttcgtcat
      541 gataagcaat atcccgaaaa atttaaagtg atcgggaaat tatctaaaac aatcctcgct
      601 agtttgtttt tagaaataag agattattag gaggaaactg atggaagatc aactcactat
      661 atttattatt tcagattctt taggggaaac agccaaagct attgcgaaag catgtttgtc
      721 acaatttcca ggacatgatg attggcattt tcaacgtttt tcctatatta acagtcagga
      781 acgcttagaa caagtttttg aagaggctag ccagaagaca gtatttatga tgtttagttt
      841 agttgatgtt gctttggctt cctatgcaca gaaacgatgt gaaagtgaac attatgctta
      901 tgttgactta ttaacaaatg ttattcaagg aatatcaagg atatcgggta tagatccttt
      961 gggagaacca gggattttac gacgcttaga caatgattat ttcaaaagag tagaatcaat
     1021 agaatttgca gtaaaatatg atgatgggag agatccgcga ggtattttac aagcagattt
     1081 agttattata ggaatttcaa gaacttctaa aacaccatta agtatgtttt tagcagataa
     1141 aaatattaaa gttattaata ttcctttggt tccagaggtt cctgttccta aagaattacg
     1201 tatgattgat tctagaagga ttatcggact aactaattcg gttgatcatc ttaatcaagt
     1261 ccgaaaagtt cgattaaagt ccttaggtct ttctagtacg gcaaattatg ccagtttaga
     1321 acggattttg gaggagacga ggtatgctga agaagttatg aaaaatttag gttgcccaat
     1381 cattaatgta tctgataaag caatcgaaga aacagcaacg attattttag aaatcttaaa
     1441 aacaaatgga caagtagcta aaaatctgta aaaggagacg ctgatggaaa ctaagtttgt
     1501 gtatcatttt gatgaaggtt gtaaggaaat gaaggaactg ctaggtggta agggggctaa
     1561 tctagctgaa atgacgagta ttgggttacc ggtaccacaa ggttttacta ttacaacaca
     1621 ggcttgtaat gattattatg ataacgcttg ccatatccga gagagtattc ttagtcaaat
     1681 tgatcaagca atggcacaat tggaggttga acagaacaag caattaggaa gtgttgatga
     1741 tcctttgttg gtgtcagttc ggtcaggatc agttttttca atgcctggta tgatggacac
     1801 agttttaaat ttagggttaa atgatcgtag tgtccaaggt ttagtgaaaa agacagaaga
     1861 cgagcgtttt gcttatgaca gttatcgtcg ttttatccaa atgtttgcag atgtggttac
     1921 aggcattcca aaatataaat ttgataccat tttagatcga cttaagacag ataaatgtta
     1981 tcaagatgac acagaactaa ctggttcaga tttaaagaga cttgttgaat tttacaagga
     2041 actatatcaa aaagaagcag gagaaaaatt tccgcaagat cctaaaaggc agcttttgtt
     2101 agcaattgag gcagttttta aatcatggaa taatccaaga gctaagattt atcgtaaatt
     2161 aaatgatatt ccggagacgc ttgggacagc cgttaatatc caagctatgg tttttgggaa
     2221 tatgggaaat aacagcggaa cgggagttgc ttttacacga aacccatcaa ctggagcagc
     2281 aaatttattt ggtgagtatt tgattaatgc acaaggagaa gatgttgttg caggtatcag
     2341 aacaccacaa tccatatcaa aattggcaga acaaatgcct attatttacc aagagtttgt
     2401 aagcgttacc caaaagttag aagctcatta tcgcgatatg caagatatgg agtttacaat
     2461 tgaaaatggt aatctttata tgcttcagac gagaagcggt aagcgtacag cgaaggcagc
     2521 tatcaaaata gcagtagatc aagtaaatga aggcctaatt agtaaagaag aagctatact
     2581 acgtatcgag cccaaacaat tagatcagtt attacaccct tcttttgact tgaaaagttt
     2641 aaagaaagct attattttga cgacaggact tccagcttct cctggagctg cttatgggaa
     2701 agtttatttc catgcagaag atgttgttaa agagatgaaa aagggaaatc cagtgctatt
     2761 ggtcagacaa gagacatcac ctgaagatat cgaggggatg gttagtgcaa acgggattat
     2821 aacagcaaga ggcggaatga cttcccatgc ggcagtagta gccagaggaa tggggaaacc
     2881 atgcgttgca ggctgtagtc aattattggt tgatgaagta agacgagaaa tttctattgg
     2941 gcatcaaacg ataaaagaag gtgagatgtt atcaatcgat ggtgctacag gaaatgttta
     3001 tataggacaa gttccgatgg cagagactag cgtagatcgt gattttgaga tatttatgaa
     3061 atgggtagat gaaaatcgag atatgatggt gtgttcaaat gcagataatc cgagagatgc
     3121 tcaaaaggcc ttggactttg gagcagaagg aataggtctt tgtcgcacgg aacatatgtt
     3181 ttttgacgat gaacgtattc cagtcgttag agaaatgatt ttagcggatg aaatattgag
     3241 ccgaaggaag gctctagaaa ggcttttatc tttccaaaga gacgattttt atcaaatttt
     3301 taaagtattg aaaggtaaag cttgtacgat tcgtttgctt gaccctcctt tacatgaatt
     3361 cttaccccat gataaagagt ctatagaatc tatggcaaga caaatgggga tatctacttt
     3421 agctattgaa aaacggattc aaacattaga agaattcaac cctatgttag gtcatcgtgg
     3481 ttgtagactt gcgattacct atccggaaat ttaccaaatg caggtgagag ctcttgtaca
     3541 aggcgctata cttgcaatga aagagggata tgaggcaaag ccagaaatta tgattccttt
     3601 agtgacagcg catgaagaaa tctctataat acgtgatctg attgaagaga caattgttga
     3661 ggagagtaaa tctaaaaaaa ttaacttatc attccctatt gggacaatga ttgaaacacc
     3721 aagagcttgt atgatagcgg atgatattgc aaagtttgcg gatttcttta gttttgggac
     3781 caatgattta acacaaatga gctttggatt ttcacgagat gatgctggaa aattcttagg
     3841 agaatacgtt gataaaggac ttctaaaaaa agaccctttc caagttttag atcaaaaagg
     3901 aataggaagg tttattgagc aggcagtgag gttaggaaaa gaagttaagc caaaccttaa
     3961 aataggtatc tgtggagagc atggtggaga accatcatct attgagttct gttaccaaca
     4021 ggggttacat tatgtatcgt gttcgccgtt taggattcct attgctaaat tagcggctgc
     4081 acaggctaaa atcaaacaaa gcagaaatga tataacaata gataaataaa cagtaaacgc
     4141 ttcttatcat aggtaagaag cgtttattat gaaaagctgg gataatgtga taacattgag
     4201 agtttaatat gctataatag tagagactat cttaaaacga aaatgataca gagataagga
     4261 gaactcatga aaatttctga agaagaagtg cgtcatgttg cccatctgtc aaaattgcgc
     4321 ttttctgatc aagagactaa agaatttgcg tcaagtttgt caaagattgt tgatatgatt
     4381 gaactcttaa atgaagttga cacagaaggc gtaccagtaa caacgaccat ggcagatcgc
     4441 aaaactgtga tgcgtgaaga tattgctcaa ccaggccata atcgtgatga cttatttaaa
     4501 aatgtaccac agcatcaaga ttattatatt aaagtacctg caattcttga ggatggagga
     4561 gatgcctaat gtcgtttaat aatcaatcaa ttgatcagtt gcatgatttt ctcgttaaaa
     4621 aagaaatttc agcaactgaa ttaacaaaag ctacattgga agatattcat gcgcgtgaac
     4681 aagcggttgg atcttttatt actatttctg atgagatggc aatagctcaa gccaaagaaa
     4741 ttgatgataa aggaattgat gctgataatg ttatgtcagg tattccctta gcagttaaag
     4801 ataatatttc aacaaagggg attcttacaa cagctgcttc taaaatgctc tataactatg
     4861 agccaatctt tgatgctacg gctgttgaaa agttatatgc taaagatatg attgtgattg
     4921 gtaaagcaaa catggatgaa tttgccatgg gaggctctac agaaacatct tattttaaaa
     4981 agacaaataa cgcttgggac catagtaaag ttccgggagg atcatcaggt ggttctgcag
     5041 cagcagtagc ttcaggacaa gtacgtttat cattgggctc tgatacaggt ggttcaattc
     5101 gccaaccggc ttcatttaat ggaatcgttg gtatgaagcc aacttacgga cgtgtatcac
     5161 gctttggttt gtttgctttc ggtagttcgc ttgaccagat tggaccgatg tctcaaacag
     5221 taaaagagaa tgcacagctt ttgacagtta tttctgggca tgatgtaaga gattcaactt
     5281 cttctgagcg tactgtaggc gattttactg ctaaaattgg tcaagatatt caaggaatga
     5341 aaattgcttt accaaaagaa tatttgggag aaggaatagc gcaaggagtt aaagaaacga
     5401 ttattaaggc cgcaaaacat ctggaaaaac taggtgcagt tattgaggaa gttagtttac
     5461 cgcattctaa atacggtgtt gctgtttatt atattgttgc ttcatcggaa gcttcttcaa
     5521 acttacaacg ttttgatggt attcgttatg gataccgtac agaaaattat aaaaacttgg
     5581 atgatatcta tgtcaataca cgatctgaag gttttggaga cgaagttaag cgtcgcatta
     5641 tgctgggaac gttcagttta tcttctgggt attacgatgc ttattataaa aaagcaggtc
     5701 aagttcgtag tttgattatt caagattttg aaaaagtttt cgcagattat gacttgattt
     5761 taggtccaac agctccaaca acagcctttg acttagattc tttaaatcac gatccagttg
     5821 caatgtacct agctgatatt ctaacaatac cagttaattt ggctggttta ccaggtattt
     5881 caatccctgc tggttttgat caaggtctac cggttggtat gcagttgatt ggtccgaaat
     5941 tcagtgaaga aacaatatat caggttgctg ctgccttcga agcaacgaca gattatcata
     6001 aacaacaacc taaaattttt ggaggtgaga actaatgaat tttgaaacag tcattggact
     6061 tgaagtccat gttgaattaa atacgaactc taaaattttc tcaccttctt ccgctcattt
     6121 tggtcaggaa caaaatgcta atacaaatgt aattgactgg tctttcccag gtgtccttcc
     6181 agtcatgaat aaaggagtta ttgatgcagg aatcaaggcg gcacttgcct tgaatatgga
     6241 tatccatcaa aacatgcatt ttgatcgtaa aaattatttc tatccagata atcctaaagc
     6301 ttatcaaatt tcacagtttg atgagcctat tggttacaat ggttggattg aaatcgaatt
     6361 agaagatggt acacgaaaaa aaattcgtat tgagcgtgcc cacctcgaag aagatgcagg
     6421 aaaaaataca catgggacag atggttattc ctatgttgac cttaatagac aaggtgttcc
     6481 actgattgaa attgtatcag aagctgatat gcgttctcct gaggaagctt atgcttattt
     6541 gacagctctc aaagagatta tccaatatac tggaatttct gacgttaaaa tggaagaagg
     6601 ttctatgcgt gtagatgcca atatttcact tcgtccttat ggtcaagaag agtttggtac
     6661 taaagcagaa cttaagaacc ttaactcatt caataatgta cgtaaaggtt tgatacatga
     6721 agaaaaacgt caagcacaag ttttacgatc tggtggtcaa attcagcaag agactcgtcg
     6781 ttttgatgaa acaacaggtg agactatttt gatgcgtgtc aaggaaggct catcagatta
     6841 tcgctatttc cctgaaccag atctaccact atttgatatc tcggatgaat ggattgatca
     6901 agttcgttta gaattaccag aatttccaca ggaacgtcgt gcgaaatatg tatcgtcttt
     6961 tggtctatca tcttatgatg ctagccaatt aacagctaca aaagcaactt ctgatttctt
     7021 tgaaaaagct gttgctattg ggggtgatgc aaaacaagtt tccaattggt tacaaggaga
     7081 agtggcgcaa tttcttaaca gtgaatcaaa atcaatcgaa gaaattggtc tgactcctga
     7141 gaatttggtt gagatgattg gcttgattgc tgacggtaca atttcatcca aaattgctaa
     7201 aaaagttttt gtgcatttag ctaaaaatgg tggttcagct gaagaattcg ttaaaaaggc
     7261 tggtttagtt caaatttcag atcctgaagt attaatccca attattcatc aagtctttgc
     7321 agataatgaa gctgctgtga ttgactttaa gtcaggaaaa cgcaatgctg ataaggcctt
     7381 cacaggttat ctaatgaaag caacaaaagg acaagctaat ccacaagttg ctctgaagct
     7441 gttagcgcaa gaattagcaa aattaaaaga agaatagaaa tcagcctttt ggctggtttt
     7501 tattcgctaa tttagcacga acacctaggg ataaataatt aagaagctaa gatatttatc
     7561 agatgacttt aactacagtg tttaagaaat aaaaatgagg ggaaaaatga ctaaaaaaga
     7621 aaaaggaaca atgatgacct tagctgccgg actagcatgg ggcatttctg gaattagtgg
     7681 tcaatacttg atgtcgcacg gagtacacgt taaccttttg acttctttac gattgttgat
     7741 aacaggtatc tttctcttgt ctttagctag atcaaaacaa aaggagcatt tagtagcagc
     7801 gtggaaacag ccaaaatttt taaagcaggt tttacttttt tctatatttg gtttagtttt
     7861 aaatcaatat gcttttctca gagcgattca tttaacaaat gctggaactg caacggtttt
     7921 gcaatatatg gcaccaatac ttattttgag tattgtttgt atcctaaaca gacaaagacc
     7981 aacatctttt gaaataatag caattgctat ggcaatttta ggaacctata tgattgcaac
     8041 gcatgggaag ttaggaagtt tagctatcac accgaaagga ttaatgtggg ggcttggttc
     8101 cgccattact tattctattt atattttatt acctgtgaaa cttattcatg aatggggaag
     8161 tacgattgtt attggttcag gaatgtttat aggtgggatt ttatttagtt tggtgacaaa
     8221 agcgtggcaa taccccttac aaattaatgt gatgagtata cttgcttata ttggcattat
     8281 tggtattggg actatttttg cttacacttt tttccttaaa ggggttagta ttgtaggtgc
     8341 tgtcaagggt agcttattag catcggtaga gccagtttct tcggtatttt tgacagtact
     8401 tgttttagga gaaatttttt atccaataga tttacttggg atgctgttta tttttcttgc
     8461 tgtaactcta atctcttaca aagatttaat tgcattgaag aaaatgaaac gagtaagata
     8521 gtatcttttg gggttgaaac cctaatttaa aggtctttaa agaccttttt tctttactcg
     8581 ctatctctta agaattatgg tagaatgaat agtaaaatat attttttgga gatgaaattg
     8641 agtatagatg attataggcc gaccttcgtg gttgaggcag tttatgattt aagagcagaa
     8701 gatttattac gtcatggcat tcgtgcggtt ttagttgatt tagataatac actcattgct
     8761 tggaataatc cagatggtac tgctgaggtg cgtgcatggt tagatgagat gacaacagcg
     8821 gatatttcag ttgtagtagt gtctaataac aatcatgctc gcgtagaacg tgcagtatca
     8881 cgttttggtg tggattttgt aagtcgtgcg atgaaaccat ttactcgtgg tataaacatg
     8941 gcaattgagc gttatggctt tgatcgtgac gaggtgatta tggttggaga tcaattgatg
     9001 acagatattc gtgctagtta tcgcgcagga ataaagtccg tattggtaaa accaattgtt
     9061 aaatctgatg cttggaatac caagttcaat cgcttacgtg aacgtcgcgt ttggaaaaaa
     9121 atagaagaga attacggtaa aatcgtatat caaaaaggaa tttaatggaa gagttatttt
     9181 gtattggctg cggagccagg attcaaactg aaaataaaga tgctgctggc tatacacctc
     9241 gtgcagcact tgaaaaaggt ttagagacag gagaattata ctgtcagcgt tgtttcagat
     9301 tgcgccacta taatgagatt actgacgttc atatcactga tgatgaattt ttgaaattac
     9361 tacatgaggt tggagatagt gatgcccttg tggtgaatgt cattgatatt tttgatttca
     9421 atggttcaat tattcctggt cttagtcgtt tcgttgcagg aaacgatgtt cttttagtag
     9481 gaaataaaaa agatattctt cccaaatcgg ttaaagatgg aaaagtaaca caatggttaa
     9541 ctgagagagc tcatgaagag gggcttcgtc ctgttgatgt catcttgaca agtgcccaaa
     9601 atcatcatgc tattaaggat ttgattgata cgattgaaaa gtatcgtcat ggtcgagatg
     9661 tttatgttgt tggggttact aatgttggaa agtcaactct tattaacgct attattcgtg
     9721 aaattactgg tagtcgtgat gttattacga cctcacgttt cccaggtaca acgttagata
     9781 aaatagaaat acctcttgat gatggtagct atatttttga taccccgggt attattcatc
     9841 gtcatcaaat ggctcattat ttgacagcaa aaaatcttaa atatgttagt cctaaaaaag
     9901 aaataaaacc caaaacttat cagcttaatt cagaacaaac attattcttg gctggcctag
     9961 cacgttttga ttttatttct ggtcaaaaac aaggttttac ggcatatttt gataataacc
    10021 tgaatttaca ccgcactaaa ctagttggag cagatgaatt ttacactaaa catgttggaa
    10081 aattattgac accaccaaca ggtaaggaag tttcagattt tcctaaatta gttcgtcatg
    10141 aatttacaat caaagataaa atggatattg tttattcggg tcttggttgg atacgtgtga
    10201 aatcagaagc agaaaatcca gtagtagtag ctgcttgggc tccagaaggc gtggcagttg
    10261 ttttacgcaa agctttaatt taataactaa attaaccagc aacatatgac ggtttttcat
    10321 ctaatttagc tgatgataca gtcataatcc tataatagat aaaaaggaaa ctaatatgtt
    10381 aacatctaaa caacgtgctt tcttaaaatc agaagcacat tctatgaaac ccattattca
    10441 aattgggaaa aatggtttaa atgatcaaat caaaacaagc gtacgtaatg cactcgatgc
    10501 acgtgagcta atcaaagtaa cccttttaca aaataccgat gaagatatcc acgatgtcgc
    10561 tgaagttctg gaagatgaaa ttggctgcga tactgtcctt aaaataggac gtatcttgat
    10621 tctttataaa gagtcagcgc gcaaagaaaa tcgtaaaatt tcagttaaag taaaagcagt
    10681 aaaatcaaac taaactacaa tagattaaga aatagagtat agaactaaaa aacgatttac
    10741 ctaagttaca cttccgtatg atagctgatc atgataatca gtgtttgttt ttttagagat
    10801 actacttgtt catggaggaa ttatggcact cgaattgcta acaccattta caaaagttga
    10861 attagaagag aaaaagcgag atactaaccg aaaacaaatt ggaattatgg gaggaaattt
    10921 taaccctgtt cataacgccc accttgttgt tgcagatcaa gtacgtcagc aattgtgttt
    10981 agaccaagtt ctgttgatgc ctgaatttca gccaccacat attgataaaa aagagacaat
    11041 tgatgaacag catcgtctca agatgcttga attagcaata gaaggcattg atggtttatc
    11101 tattgaacca attgaaattg aacgtaaatg tatcagctat acatacgata cgatgaagct
    11161 tctaattgaa aaaaatccag atgttgatta ttatttcatt attggggcag atatggtgga
    11221 atatctacct aagtggcatc gtattgatga acttgttaaa atggtgcagt ttgttggtgt
    11281 tcaacgtcca aaatataaag ctggtacatc atatcccgtt atttgggtgg atctgccatt
    11341 gatggatatc tcatcaagta tgattcgtca atttattaaa agtaatcgtc agcccaacta
    11401 tttactgcct agagaggtat tagattatat caggaaagaa ggtctttaca aatgacctac
    11461 aaagattaca caggtttaga tcggactgaa cttttgagta aagtgcgtca tatgatgtcc
    11521 gacaaacgtt ttaatcatgt gttaggagta gagcgtgctg caatagagtt agcagaacgg
    11581 tatggttatg acaaggaaaa agcgggtcta gctgctttat tacatgatta tgctaaggaa
    11641 ctatctgatg atgaatttct tagattaatt gataaatacc aactagatcc cgacctaaaa
    11701 aaatggggta ataatatttg gcatggtctt gttggcattt ataaaataca agaggatcta
    11761 gctatcaaag atcaagatat tttagctgct attgcaaaac atactgttgg ttcagcccaa
    11821 atgtccacat tggataaaat cgtttatgta gcagattaca ttgaacacaa tcgagatttt
    11881 ccaggagttg aagaagctcg tgagttagcg aaagttgacc ttaataaagc agtagcttat
    11941 gagactgcta gaacagttgc gtttttagca agtaaggctc agcctatcta tcctaaaacg
    12001 attgaaacct ataatgctta tataccttat ctcgactaat tcttttattg aataatggtc
    12061 atgataggtt tggcgatgca gtggtcattt tggaggaaag tatgacttgt gcacttttac
    12121 taattgatat ccaacaaggt attatggata agaaaccaaa gcatttaaca aattttgcag
    12181 agcttttaga tgatttactt ttaagtgcca aaggttctaa ttgtgaagtg atatggataa
    12241 ggcatcatga taaggaattg cctcaaggtt ctccacagtg ggaaatttgg gaacagcgac
    12301 atcttgtgac gcatcacaaa ataattgata aaacttacaa tagttgtttt aaagatactc
    12361 atttacatga ttacttgcaa agtaagcata ttagtcaatt aatcatgatg ggacttcaaa
    12421 cagaatactg ttttgataca tcagtaaaag ttgcttttga atatggttat gatatcttta
    12481 ttccacaagg tggacaccta acatttgata caccaacttt aagtggggat tctattaaaa
    12541 aacattacga gaatatttgg catcatcgtt ttgcgacaat ggtagcaaaa gatagcttat
    12601 tgaaattaat aaacgaggaa taaaatgaca gaaaaagact tactacagct agtcgttaag
    12661 gctgcggatg aaaaacgagc agaagatatt gtaattttag atttacagcc tgtaacgagt
    12721 gttgcagatt actttgtgat tatgagtgca agtaatagta ggcagttaga agcaatcgca
    12781 gacaatatcc gtgaacaagt aaaaggaaat ggtggggatg ctagccacct tgaaggcgat
    12841 agtaaagctg gttgggtact tcttgacctc aatagcgtcg tggttcatat cttctctgaa
    12901 gatgagagac agcactataa ccttgaaaaa ctttggcatg aagcaccatt gcttgatgct
    12961 gaagtcttta tgacagaata aagctaagca gtcttgggac tgctttttga gagatgtaat
    13021 atcaagaaag atttgagaac tattatatgg caacgtatga aacttttgca gctgtttatg
    13081 atgctgtcat ggatgatact ttatatgata aatggactga tttttcactt cgtcattttc
    13141 caaaaggtaa gaaaaaactt ttggaattag catgtggtac gggaattcag tctgttcgat
    13201 ttgcacaagc tggttatgct gttacaggac ttgatcttag tggcgatatg ctgaaacttg
    13261 ccaaaaaacg tgcaacttct gctcaccaaa gtatccagtt tattgaagga aatatgttag
    13321 atttatctaa tgttggtaaa tatgatctca tcacgtgcta ttcagactct atttgttata
    13381 tgcaagatga agtcgaggtt ggtgatgttt ttatcgaagt ctataaggcc ttagaagaaa
    13441 atggcgtctt tatttttgat gttcattcga cttatcaaac agataaagta tttcctggtt
    13501 attcttatca tgaaaatgca gatgactttg ctatggtgtg ggatacttat gaagatgatg
    13561 cacctcattc tattgtccat gaattaacat tttttgttca agaagaagat ggtcgattta
    13621 cacgtcatga tgaagttcat gaagagcgta cttatgatat tttgacttat gatattttac
    13681 ttgaacaggc aggttttaaa gatgttaagg tgtatgcaga ttttgaagat aaaaaaccaa
    13741 ctgcaacttc tgctcgctgg ttttttgtag ctcataaata agtaataatt tggaggtaaa
    13801 aagatgataa tattcttata cgcttgtctg atgatatttg ggatacttta tattattgaa
    13861 cgtcgggtaa gtcagaaaaa gtctaaagtt attttggaat tattgatgaa ccctaaactt
    13921 catttggatt tattatcggc attaaaacca tttgaaaaag aaacagatcg tctcatatac
    13981 cttcgtaaaa cttatcacct agatattatt gatgctgtta ctttaaataa tcaattgaaa
    14041 gagaaagaag tatgacagtt acaggaattg ttgccgaatt taatcccttt cataatgggc
    14101 ataagtacct tttagaacaa gctcaaggca taaaagtaat agccatgtcg ggtaatttta
    14161 tgcaacgtgg tgagccggcg attgtagata aatggacccg tgcccagatg gcactagaaa
    14221 atggtgctga tttagtgatt gaactccctt ttctggtttc ggtacagtca gcggattatt
    14281 tcgctagtgg agctgtgtct atcctggcac gattaggtgt ggataactta tgcttcggta
    14341 ccgaagaaat gcttgattat gcccgtattg gggatattta tgtgaataaa aaagaggaga
    14401 tggaagcttt tttgaaaaaa caatcggatt ctctttccta tccacaaaaa atgcaagcta
    14461 tgtggcaaga atttgcaggg attacatttt cgggacaaac cccaaatcac attttaggct
    14521 tagcctacac caaagcagct agccaaaatg gtattcgatt aaatccgata caacgtcaag
    14581 gagctggtta tcattcatca gaaaaaacag agatatttgc ttctgcaaca agtcttcgta
    14641 aacatcaatc tgatcgtttc tttgtagaaa aaggtatgcc taacagtgac ttgtttttga
    14701 atagtccaca agtagtttgg caagattatt ttagtttact caaatatcag ataatgacgc
    14761 attctgattt gacacaaatt tatcaagtga atgaagagat agccaatcgt ataaaaagtc
    14821 aaattcgtta tgtggagact gtggatgagt tagttgataa agtcgcaact aaacggtata
    14881 ccaaagcaag aattcgacgt ttattaacct atatcttgat aaatgctgtg gaaagtccta
    14941 taccaaatgc tattcatgtc ttaggtttta cacaaaaagg tcaacagcat ttaaagtctg
    15001 tgaagaagtc tgtggatatc gtgacgagga ttggttcaca aacgtgggat agtttgacgc
    15061 aacgagcaga ttctgtttat cagatgggaa atgctaatat agcagagcaa acatggggac
    15121 gtatcccttt ttatcaacct gttagtcaaa gtgacctttg atttaagctt tataaattaa
    15181 tcatcacaaa ataatatttt tcccttgacc cttacttaac gttatacatt atactcttta
    15241 cagagaggtt attatgaaaa cagttaaaga aataagtcac atttctggta ttagtgtgcg
    15301 cacacttcat tattatgatg aaatagattt gttatctcct tcatttgtag gagagaatgg
    15361 ttatcgttac tatgatgatg aatcactgat taagttacaa gaaattttgt tatttaagga
    15421 actggaattt cctcttaaaa aaataaaaga gataatggaa agtccaaatt atgatcgtaa
    15481 tcaagcactt cttgatcaaa ttaggtggct ggaactaaag aaacagcgac tagaagaagt
    15541 aattgaacat gcaaagtcta tccagagggg taaaaatatg tcagatttta cagcttataa
    15601 tcaagaagaa ttagaagtat ttcaagagga agctagaaca cgctgggggg gatacagata
    15661 gttacaaaga atttgaaaat agccatagta agaatgattt ttcaatgatt tcacaagcta
    15721 tgagtcagat ttttaaagac tttggacagt tgaaagaact ttctccgaca gatgagaaag
    15781 ttcaaaaaca agttcagatt ttacaagatt atatcacagc tcagttttac aactgcacta
    15841 acgatttatt agcaagtctt gaaataatgt acatctaaga tgagcgtttc caaaagtcta
    15901 ttgataattg gggaggccag ggaacagcat tatttgtttc taaagctata gatagctatt
    15961 gtcattaggc tatattttag gtttgcaatg aaatgatagg aatcaaggaa cagtagatat
    16021 tattaaaaat gtcagactct tatctaaatt tttgacagta aggagcaaaa agagtagact
    16081 gtaggcatag aaaggtaggt atcgttatga aaggatttca tgggaatatc gaaaaattga
    16141 cactaggaaa tacaaatttc cgccaagtcc tctataccgc agaacattgt caactcgttt
    16201 taatgacact gcctgttggt ggagaaattg gtagcgagat tcatgcagaa aatgatcagt
    16261 ttttccgttt tgaagctggt catggtaagg ttgtcattga tggtaatgaa tatgaagttg
    16321 cagatggaga cgctataata gttccggcag gtgctgaaca taacgtaatt aacacttctg
    16381 aaactgagat gttaaaactc tatacaattt acagtccagc tcatcacaaa gatggtatta
    16441 tccgtgccac aagagaagaa gctgaagaaa atgaagaaga ttttgatgga gttactactg
    16501 aaactaaata atactagact aaaaagcgct atgtcaatag cgctttttag cttaatagta
    16561 gtcatttctt ggagaatata aatagtaatt taggaagtaa tgttaataac cgagctttta
    16621 aggaaacgtt aagtaagggc ggttatacta taaacatcct aaactttttt cataaaatct
    16681 ccagaaagaa cactaagtat tttggtgttt tttctatttt gaaagcatac tgcaactatg
    16741 aaaaaccaga aagtattaac tttctggttt tttagttaaa ctaaagtcaa agcaatattg
    16801 tataagatag ccccaaggac accgccgata attggaccta caactggaat ccaagcgtat
    16861 gaccaatctg agttaccctt attttcaata ggtaagatag catgcataag acgaggccct
    16921 aggtcacgtg cagggttaat agcataacca gttgttggac caagagagaa tcctactgcg
    16981 aaaataacta taccaacgat aattggatca agacctgctg caactttact aggaccaatt
    17041 gccataattg taatcacaag aatagcagta ccgataattt caccaaggag attcgatggt
    17101 gtatggcgga tagcaggtcc ggttgagaag ctggcaagga taagtccact atcttttgtt
    17161 tctttccaat gaggataata gtgtaaccat aaaatagttg ccgcaaccat tgctcctaac
    17221 atctgtgcaa tgataaaggg gataacttgt ccccaagaaa ttactccaat agaagccatt
    17281 gctaatgtga ctgctggatt tagatgagcg ggactgaata atccagaaac ataaacagca
    17341 acggtacaag caattcccca gccaaaaaca atagcagtcc atccagttcc ttcttctttg
    17401 gttttactga gcacatttcc tgcaactacg ccatccccaa gaagaaccaa aataaatgtt
    17461 cctaaaaatt ccccaagaaa ttgagtcata atatctcctt atttattgat agttaattca
    17521 gctaattgtg attgaatact atccaggtca gcacctgcct gtagaagagc agcagcggtg
    17581 taagctccct ccactactgg gacagagtta atgataatat ttttttctga aaaatcagct
    17641 actaactcta aattcatttt tgctgaacct aaatcaaaga aagctaataa cgtcttttta
    17701 tcattttgct ctacaatctg ttgaacttga tcaaaagaag taccaatctc accatcttct
    17761 gttccaccca cgtaagttat tgagacatcc tttgctacct ctgaaataag atcaacaact
    17821 ccttgagcaa tatttttaga gtgagaaaca acaataatac caatatcaga cattagacac
    17881 ctccaacttc tagtaatgct ttaaataata atcctgaaga aaatgatcct ggatcaatgt
    17941 gtcctactga gcgttctcca acataagaag cgcgtccttt tgttgctttc atcccttttg
    18001 tagcatctac taaggaatcg atacggtctt cagtcaattc atttttttca atcgcttcaa
    18061 taacaccgtg ccaaacatca accattgttt tttcattaag ttcagcttta ccacgttttt
    18121 gaatcatttc aagtcctgcg ccaagtgcct ctgaaatggt actttcactc tgttcagctt
    18181 ttgtaattcc cataaatgca gaaccataga gtggtccaga tgctccgcct actttgctta
    18241 aaagttgcat ggacacggtt ttgaaaacat cggcagcact actaaattca ttgttatcaa
    18301 ggttttcaat aacagcagtc ataccacgcg ccatattgcc accatggtcg ccatcaccta
    18361 ttggagtatc tagttcggat aaatagtcct tgttggattg aattttttgg ttaaaagtgt
    18421 gcatccattc tattgcagtt tttacttcca taagagttcc ttccttttgt tctaattaac
    18481 aggaatgatt accaagcaat ggttgtaacg tcactattaa gtgcttctaa ccactcttga
    18541 ttttccagtt taatcaaggt tagtgataaa ccagccatat caattgaagt catatagtta
    18601 ccaagttttt tgtaattcac ttcaatgcct ttatcctcaa ggagtttggc aacatcgtta
    18661 gcaaagacat attgttccat aagtggggtt gcacccatac cgttaataag gacaccatat
    18721 ttttcgccag atttagcatc gaacgattca attaacttat caactaattc tgtcgctaaa
    18781 gcctttgaag gttgcatttt ttctttacga taacctggtt caccatgaat accgatacca
    18841 aattcaattt catcttctgc aagaacaaat cctggcttgc ctacttcagg aacagtagcc
    18901 ccgcttagtg caagtccaac agtgtgaatg tttggtacta attcatcagc aatagctttg
    18961 atttcctgaa gtgatttgcc atgacgagca gcatgtccta gaattttatg gacgagtatc
    19021 gtaccagcta caccacgttt tccttgtgtg taaagactgt cttcgactgc aatatcatca
    19081 tcaacgacca cacttgcaac ttctatgcct tccatttcag ccatatcttg tgccatttca
    19141 aagttcatga tatcccctga ataatttttg ataaccatga aaactccagc cccctcatca
    19201 gcttctttaa ttgcttcaag aacttggtca ggagttggag aagtaaatac tgcaccacac
    19261 acagctgcag ataacattcc ttcgcctacg aaaccagcat gtgaaggctc atgtccactt
    19321 cctccgccag aaatgagagc cacttttcct gacttctctt cattgcgagc aatgatatca
    19381 aaaccttcta tgcgatgtac taaatcatta tgaacatatg ctaatccatc tagcatttca
    19441 gtaacaacat cagttggttg attgagaatt tttttcattt ggaaactcct tttatgttta
    19501 attctctttt attataaaat ttgtaagcct tttctggaat ggacagattt tcttgtttgt
    19561 ccgctttgtt agggttttca ataatcataa atcatgttac actaaaccat aataaatcat
    19621 cagcaattca atataaaata aaggagaaat tatgacttct tcgcttatta ctaagaaaaa
    19681 aatagccaag tcatttaaac gtttatttat ttcgcaagct tttgacaaaa tatcggtgag
    19741 tgatattatg gaagatgcag gtattagacg tcaaacattt tataatcact ttgttgataa
    19801 atatgcttta ttagaatgga ttttccaaac agaattaagc gaacaagtta cggataattt
    19861 agactatatc tcaggttttc agttgttatc agag
//
LOCUS       DATRQA010000025        19371 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.025, whole genome shotgun sequence.
ACCESSION   DATRQA010000025 DATRQA010000000
VERSION     DATRQA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19371
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.025"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(56..307)
                     /gene="yidD"
                     /locus_tag="VBD27_000646"
     CDS             complement(56..307)
                     /gene="yidD"
                     /locus_tag="VBD27_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO7024677.1"
                     /translation="MLKSFLIFLVRFYQKNISPAFPASCRYRPTCSTYMIEAIQKHGL
                     KGVLMGIARILRCHPLAHGGNDPVPDHFSLRRNKTDISD"
     gene            complement(307..1029)
                     /locus_tag="VBD27_000647"
     CDS             complement(307..1029)
                     /locus_tag="VBD27_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7024678.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGMVTINGQVVNELATQVKAG
                     DLVEIEGSPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVIDLLPQVKERIYPVGRLDW
                     DTTGLLILTNDGDFTDKMIHPRNEIDKVYLARVKGIATKENLRPLTRGVVIDGKKTKP
                     ARYTIIKVDHEKNRSVVELTIHEGRNHQVKKMFEQVGLLVDKLSRTQFGTLDLTGLRP
                     GEARRLNKKEISQLHNAAINKS"
     gene            complement(1019..1603)
                     /locus_tag="VBD27_000648"
     CDS             complement(1019..1603)
                     /locus_tag="VBD27_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEO7024679.1"
                     /translation="MTYLGSIEALLFVAGEDGLSLRQMAELLSLTPSALIQQLEKLAK
                     RYEEDDDSSLLLLETAQTYKLVTKDSYMTLLRDYAKAPINQSLSRASLEVLSIIAYKQ
                     PITRIEIDDIRGVNSSGAITRLIAFGLIKEAGKKEVLGHPNLYETTNYFLDYMGINQL
                     DDLIDASSIELVDEEVSLFSMDSINTEDKENNEN"
     gene            complement(1600..2307)
                     /locus_tag="VBD27_000649"
     CDS             complement(1600..2307)
                     /locus_tag="VBD27_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO7024680.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYEVDIYDVPIVEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMLIKSRNLLPKVVESNPIEDDPEMELLSQLEEYRRFKVL
                     SEELANQHQERAKYFSKPKQEVIFEDAILLHDKSVMDLFLTFSQMMSQKQKELSNSQT
                     VIEKEDYRIEDMMIVIERHFNLKKKTTLQEVFADCQTKSEMITLFLAMLELIKLHQIT
                     VEQDSNFSQVILRKEEK"
     gene            complement(2307..3047)
                     /gene="xerD"
                     /locus_tag="VBD27_000650"
     CDS             complement(2307..3047)
                     /gene="xerD"
                     /locus_tag="VBD27_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO7024681.1"
                     /translation="MINDINNFIESKKLSLNSRKSYHYDLKQFYKIIGGHVNSEKLAL
                     YQQSLSEFKLTARKRKLSAVNQFLFFLYNRGTLKEFYRLQETEKITLAQTKSQIMDLS
                     NFYQDTDYPSGRLIALLILSLGLTPAEIANLKKADFDTTFNILSIEKSQMKRILKLPE
                     DLLPFLLESLEEDGDLVFEHNGKPYSRQWFFNQLTDFLNEKNEQQLTAQLLREQFILK
                     QKENGKTMTELSRLLGLKTPITLERYYR"
     gene            complement(3037..3510)
                     /locus_tag="VBD27_000651"
     CDS             complement(3037..3510)
                     /locus_tag="VBD27_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7024682.1"
                     /translation="MIAKEFESFLLNHLDHYLIPAEDVAIFVDTHNADHVMLLLASNG
                     FSRVPVITKEKKYVGTISISDIMGYQSKGQLTDWEMAQTDIVEMVNTKIEPINEAATL
                     TAIMHKIVDYPFLPVISDQNDFRGIITRKSILKAINSLLHDFTDEYTITPKNNDK"
     gene            complement(3507..4028)
                     /locus_tag="VBD27_000652"
     CDS             complement(3507..4028)
                     /locus_tag="VBD27_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO7024683.1"
                     /translation="MAIRKLVVMSDSHGDRDIVKDIKNHYLGKVDAIFHNGDSELPSS
                     DPIWEGIHVVTGNCDYDSGYPEVLVTKIDNAVIVQTHGHLHQINFTWDKLDLLAQQED
                     ADICLYGHLHRADAWKNGKTIFINPGSVLQPRGPINEKLYAVVTITDSKVLVEYYTRQ
                     HQPYPNLTKEFSR"
     gene            complement(4010..4984)
                     /locus_tag="VBD27_000653"
     CDS             complement(4010..4984)
                     /locus_tag="VBD27_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEO7024684.1"
                     /translation="MTKTIFESKTEGNWFLGSFQAFNYFTCFGNDESYEAIQDVFHRL
                     LSTLKVEGLQLHVVQMTSDFQLLAFLVDMINQEYSRHIKVTQHKGAILVSEDDQLFLV
                     HLPKEGTSLEKFFDLKNDNNFGDTILIATHNEGKTKEFRELFGKLGLKVENLNDYPDL
                     PEVEETGMTFEENARLKAETISKLTGKMVISDDSGLKVDALGGLPGVWSARFSGPDAT
                     DARNNAKLLHELAMVFDKERRSAQFHTTLVVSAPNKESLVVEAEWPGYIGTEPKGENG
                     FGYDPLFIVGEGSRTAAELSAQEKNNLSHRGQAVRKLMEVFPKWQLEN"
     gene            complement(4981..5775)
                     /gene="racE"
                     /locus_tag="VBD27_000654"
     CDS             complement(4981..5775)
                     /gene="racE"
                     /locus_tag="VBD27_000654"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO7024685.1"
                     /translation="MDSRPIGFLDSGVGGLTVVKEMFRQLPEEEVIFIGDQARAPYGP
                     RPAQQIREFTWQMVNFLLTKNVKMIVIACNTATAVAWQEIKEKLDIPVLGVILPGASA
                     AIKSTNLGKVGIIGTPMTVKSDAYRQKIQALSPNTAVVSLACPKFVPIVESNQMSSSL
                     AKKVVYETLSPLVGKLDTLILGCTHYPLLRPIIQNVMGAEVKLIDSGAETVRDISVLL
                     NYFEINHNWQNKHGGHHFYTTASPKGFKEIAEQWLSQEINVERIVL"
     gene            complement(5950..6189)
                     /locus_tag="VBD27_000655"
     CDS             complement(5950..6189)
                     /locus_tag="VBD27_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEO7024686.1"
                     /translation="MSTTIWILLIIVALFGGLVGGIFIARKQIEKEIGEHPRLTPDAI
                     REMMSQMGQKPSEAKVQQTYRNIVKHAKTAIKTKK"
     gene            complement(6347..6889)
                     /locus_tag="VBD27_000656"
     CDS             complement(6347..6889)
                     /locus_tag="VBD27_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024687.1"
                     /translation="MSKKTTQMVSYTSILVAFAIMIPIIMPAKIIIGPASFTLASHVP
                     LFLSIFMSVPVAILVALGTGLGFLLAGFPIVIVLRALSHIGFALIAAFLIKSKPSLLM
                     SKWQTLLFAVAINIIHGLLEFITVYIITMTSNSSSTYLWSLFSLIGLGSLLHGLVDFY
                     IALFIWKWMTQKLGKTLRIR"
     gene            complement(6951..7472)
                     /locus_tag="VBD27_000657"
     CDS             complement(6951..7472)
                     /locus_tag="VBD27_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEO7024688.1"
                     /translation="MKAQERRENILTTLKGTKEAISASTLAKIFSVSRQVIVGDIALL
                     RAQQCDIISTPKGYLMSSALSTHQFTARLVCQHGIEQTEEELEIILRYQGIIMNVEVE
                     HPIYGMLTAPLNIQSQKDIDNFTAKLKVSNAELLSSLTDGLHTHMISCQDQSVFDQIC
                     EALKKAGILYIDK"
     gene            complement(7540..8229)
                     /locus_tag="VBD27_000658"
     CDS             complement(7540..8229)
                     /locus_tag="VBD27_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO7024689.1"
                     /translation="MNDNVIYTQSDSGLNQFFAKIYGLVGIGVGLSAAVSAIMLYMFP
                     QNMIAIMQKMPGLYFGAIILELVLVFVASGAARRNTPAALPLFLIYSALNGFTLSFII
                     ARYTQTTVLQAFITSAAVFFAMALIGAKTKKDLSGMRKALMAALIGILIASLVNLFIG
                     SGGMSYIMSIVCVIIFSGLIAYDNQMIKYVYNSQGGQVADGWAVSMALSLYLDFINLF
                     LNILRLFARND"
     gene            complement(8252..8755)
                     /locus_tag="VBD27_000659"
     CDS             complement(8252..8755)
                     /locus_tag="VBD27_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7024690.1"
                     /translation="MKKYQDDKDFMDLVGHLIDHPRFQKLEAIVQHHHSTRLEHSINV
                     SYTSYKIAKKFGWDASSTARGGLLHDFFYYDWRVTKFNKSHAWVHPRIAVRNARKLTD
                     LNAREEDIILKHMWGATIAPPRYKESYIVTMVDKYWAVREASRPLKRIFKKPIRFSRK
                     FLGSHNH"
     gene            complement(8793..9536)
                     /locus_tag="VBD27_000660"
     CDS             complement(8793..9536)
                     /locus_tag="VBD27_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEO7024691.1"
                     /translation="MTTIITSKSNNLIKKTKKLLQKKYRKSSYLIEGWHLFEEAEKYG
                     AQFLNIFVTETAIDRLRKPERAIVVTDDVLKELTDSQTPQGIVAEIAFQETRWTDIKK
                     GRFLVLEDVQDPGNLGTMVRTADAANFDAVFLSQKSADLYNQKTLRSMQGSHFHLPVF
                     RVEIEQFVNFCKAEGITMIATTLSEQSVNYKNLPKYDYFALIMGNEGQGISKTMTEEA
                     DVLAHIEMPGQAESLNVAVAAGVVIFSLI"
     gene            9568..9846
                     /locus_tag="VBD27_000661"
     CDS             9568..9846
                     /locus_tag="VBD27_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO7024692.1"
                     /translation="MKKVHLIVSGRVQGVGFRYATYSLALEIGDIYGRVWNNDDGTVE
                     ILAQSTDSNKMTQFIQKIRKGPSKWSKVTYVDIKLDNFDDFNDFKMSN"
     gene            9931..10863
                     /gene="yidC"
                     /locus_tag="VBD27_000662"
     CDS             9931..10863
                     /gene="yidC"
                     /locus_tag="VBD27_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO7024693.1"
                     /translation="MKKTLKRILFSSLSLSMLLLLTGCVSVDKAGKPYGVIWNTLGVP
                     MANLITYFAQHQGLGFGVAIIIVTVIVRVVILPLGLYQSWKASYQAEKMAYFKPLFEP
                     INERLRNAKTQEEKLAAQTELMTAQRENGLSMFGGIGCLPLLIQMPFFSAIFFAARYT
                     PGVSSATFLGLNLGQKSLILTVIIAILYFVQSWLSMQGVPDEQRQQMKTMMYLMPIMM
                     VFMSISLPASVALYWFIGGIFSIIQQLVTTYVLKPKLRRKVEEEYTKNPPKAYKANNA
                     RKDVTNSTKATESNQAIITSKKTNRNAGKQKRRG"
     gene            complement(11063..11728)
                     /locus_tag="VBD27_000663"
     CDS             complement(11063..11728)
                     /locus_tag="VBD27_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7024694.1"
                     /translation="MINWDAIFNLELAVKAFPSVIQGLPYTIGLSLVGFILGAIVGFF
                     VALMKMSHFRLLRYLANIHISLMRGIPLMVLLFLIYFGLPFIGIQLDAVTASIVGFTM
                     MSSAYISEIIRAALLAVDHGQWEAARALGLKTPTIYRGIIIPQATRIALPSLSNVLLD
                     MVKGSSLTAMITVPDIFNNAKIVGGTYSDYMTAYILVALIYWVICTLYAIIQDWWEKR
                     LLY"
     gene            complement(11752..12609)
                     /locus_tag="VBD27_000664"
     CDS             complement(11752..12609)
                     /locus_tag="VBD27_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7024695.1"
                     /translation="MKKTILLGLVGLSAMTLAACSNGQSNKETTWDNIKKDGVLKVAT
                     PATLYPTSYYDDHKKLTGYEIDMMKAIAKKLKIKVKFVEVGVAESFTSVDSGKVDVAV
                     NNFDTTPERLKKYNFSQPYKYSVGGMIVRADGSSKITAKDLSDWKGKKAGGGAGTQYM
                     KIAKQQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVIAVKYVTKQYPDIKVKM
                     GDVKYNPTEQGIVMSKKDKSLKTKIDAAIKDMKKDGSLKKISEKYYAGQDLTKEPAGT
                     DKIPVIKVK"
     gene            complement(12770..14230)
                     /locus_tag="VBD27_000665"
     CDS             complement(12770..14230)
                     /locus_tag="VBD27_000665"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO7024696.1"
                     /translation="MVFKDATAMVQAIKQHKISSQELVEQAIYKIEEQNVSVNAVVSK
                     QYNEARQAAKYANESNAPFAGVPILLKDLGQNQKGQLSTSGSQLFKHYHAKQTDYLVQ
                     SFEKLGFIILGRTNTPEFGFKNISDGQLHGNVNLPFDHSRNAGGSSGGAAAAVSSGMV
                     PIAGASDGGGSIRIPASFNGLIGLKPSRGRIPVGPSSYRGWQGASSHFALTKSVRDTK
                     RLLYYLQSYQVESPFPLKKLSKESLFEFSVSKPLKIAVLMDSPLKTKVSSEAKAAIKE
                     AADFLSQKGNHLELVEQPLDGIHSMKTYCMMNSVETAAMFDDIEKSLGRSMEFSDMEL
                     MTWAMYQSGQRVLAKDYSKLLDSWDQFAATMARFHENYDLILTAATNQPAPFHGQFDL
                     DETLQKQLRHMGEFSVSEQQDLIWKMFEDSMAWTPFTHQPNLTGQPSLAIPTHLTKEG
                     LPLGVQLTAAKGREDLLLAVAELFEKEKQFKGPVYH"
     gene            complement(14334..14816)
                     /gene="greA"
                     /locus_tag="VBD27_000666"
     CDS             complement(14334..14816)
                     /gene="greA"
                     /locus_tag="VBD27_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO7024697.1"
                     /translation="MAEKTYPMTQVEKDQLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQIQILETKIRYAEIIDSDAVAKDEVAIGKTVLVQEVGTND
                     KDTYHIVGAAGADIFSGKISNESPIAHALIGKKTGDLATIESPAGSYQVEIISVEKTN
                     "
     gene            complement(14889..16691)
                     /gene="mltG"
                     /locus_tag="VBD27_000667"
     CDS             complement(14889..16691)
                     /gene="mltG"
                     /locus_tag="VBD27_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO7024698.1"
                     /translation="MTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQ
                     EAKEAARRTAQLMADYEAQRLKDEQEARAKALETKQRLEEQEKARIEAKLLAEAAREE
                     ERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKTDVATAV
                     PNQETEEIFLVRATDIPTEGENVKLGETSELEPVAKEPIRVEDLSKEEEGIALSAKNK
                     HNKRERRQKADNVAKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVE
                     IPIGSGNKLIGQILEKAGVIKSATVFNYYSKFKNYSNFQSGYYNLKKSMTLDQIAAEL
                     EKGGTAEPTKPALGKILITEGYTIKQIAKAIESNKIDTKTTSTPYKADDFLKLVQDET
                     FIKKMVAKYPNLLGSLPDKSKAIYQLEGYLFPATYNYYKDTTLEGLVEDMISTMNTKI
                     APYYNTIKAKNMSVNDVLTLSSLVEKEGSTDEDRRKIASVFYNRLSAGQALQSNIAIL
                     YAMGKLGDKTSLAEDAQINTSIKSPYNIYTNTGLMPGPVDSPSISAIEATIKPASTDY
                     LYFVADVKTGNVYYAKDFETHKANVEKYINSQIN"
     gene            complement(16778..17281)
                     /locus_tag="VBD27_000668"
     CDS             complement(16778..17281)
                     /locus_tag="VBD27_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7024699.1"
                     /translation="MIIRNGCLEDLQQVISIEQINFSEAEAASKKAMQERLTIMTDTF
                     LVAEINGRLAGYIEGPVIKGRYLTDDLFHKVSEFPVRVGGFIGITSLSIHPDFKGQGI
                     GTALLAAMKDLVVSQERDGISLTCHDDLISFYEMNGFKDEGESDSQHGGSLWYNMIWN
                     NPTRSIC"
     gene            complement(17367..18698)
                     /locus_tag="VBD27_000669"
     CDS             complement(17367..18698)
                     /locus_tag="VBD27_000669"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO7024700.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHNVQGSDVDKYYFTQRGL
                     EQAGVTILPFSPNNISEDLEIIAGNAFRPDNNEELAYVIEKGYHFKRYHEFLGDFMRQ
                     FTSLGVAGAHGKTSTTGLLAHVLKNITDTSFLIGDGTGRGSANANYFVFEADEYERHF
                     MPYHPEYSIITNIDFDHPDYFTGLEDVFNAFNDYAKQVQKGLFIYGEDPKLHEITSEA
                     PIYYYGFEDSNDFIAKDITRTVNGSDFKVFYNQEEIGQFHVPAYGKHNILNATAVIAN
                     LYIMGIDMALVAEHLKTFSGVKRRFTEKIIDDTVIIDDFAHHPTEIIATLDAARQKYP
                     SKEIVAIFQPHTFTRTIALLDEFAHALSQADSVYLAQIYGSAREVDNGEVKVEDLAAK
                     IVKHSDLVTVENVSPLLNHDNAVYVFMGAGDIQLYERSFEELLANLTKNTQ"
     gene            complement(18708..19325)
                     /locus_tag="VBD27_000670"
     CDS             complement(18708..19325)
                     /locus_tag="VBD27_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024701.1"
                     /translation="MNRGSRRFPLIADDEPVMSPLVKMNLYDNEDLINNIRGFYQEKT
                     YQSMVKSNYEHEEISHPKVIENDPVPPQSFVKKATELSKSRQEAKRSVREKRQAYYAK
                     QEFKAPSKEAFQQQLKATVPKKQTQRKVTELSHLSDRLQQESYILAEIPIIFQEPDNT
                     PNPKTKKNNFDFLKRSQVYNKQDNQFHKERAKAQELNLTRFKDIN"
BASE COUNT         6035 a         3822 c         3014 g         6500 t
ORIGIN      
        1 tgtttattcc taaaacatgg tcaaaactat aattacaaat aataaaacgg atatatcaat
       61 ctgatatatc cgttttatta cgtcttaagc taaaatgatc agggacagga tcatttcctc
      121 cgtgggctaa gggatgacat cgcaaaatac gtgcaatccc catcaacaca ccttttagac
      181 catgtttttg aatagcttct atcatatacg tagagcaagt tggacgataa cgacagctag
      241 ctgggaaagc tggagaaata tttttttggt aaaagcgaac taagaaaatc aaaaaagatt
      301 taagcattaa gatttattaa tagccgcatt atgcaactga ctaatttctt ttttatttaa
      361 tcgtcgtgct tccccaggac ggagacccgt taaatctaag gtaccgaatt gtgtacgaga
      421 tagcttgtcc actaataacc caacttgttc aaacattttc ttaacttgat gattacgacc
      481 ttcatgaata gttaattcaa ctactgaacg atttttttca tgatccactt ttatgatagt
      541 atagcgagca ggttttgttt tcttaccatc gataacaacg ccacgcgtta atggtcgaag
      601 gttttcttta gtagctattc ctttaacacg tgctaaataa actttatcaa tttcattacg
      661 aggatgaatc attttatcag taaaatcacc atcattagtc aaaattaaaa gtccagttgt
      721 atcccaatcc aagcgaccaa caggatatat ccgttctttg acttgtggca acaagtcaat
      781 aactgtctta cgtcctttat catctgagac acttgaaata acaccacgtg gtttatttaa
      841 gagataatat accttctctt cgttgtaaat tggacttcct tcaatttcaa ccagatcacc
      901 agcctttact tgggtcgcca attcattaac cacttgacca ttaatcgtga ccattccttg
      961 cttgattaat tcctcagctt ttctacgact tgcaatccct gcatgcgcaa tatatttatt
     1021 aattctcatt gttttcctta tcttctgtat taattgaatc cattgaaaat aacgatactt
     1081 cttcgtcaac taattcaatt gaagaggcgt caattaagtc atctaactga ttaattccca
     1141 tataatctaa gaaataatta gttgtctcat aaaggttagg atgtcccaac acttctttct
     1201 ttccagcctc tttaattaaa ccaaaagcta tcagtctcgt tatagctcca ctggaattca
     1261 cgccacgaat atcatcaatt tcaatcctag taataggttg tttataagca ataatagata
     1321 gaacttccaa acttgcccga gatagactct gattaatcgg agccttcgca taatctctca
     1381 acaaagtcat ataactatcc tttgttacca atttataagt ttgtgctgtc tcaagcaata
     1441 atagactaga atcgtcatct tcttcatatc gtttagccaa cttttctagt tgctgtatta
     1501 atgctgatgg tgtcaaagac aataattctg ccatttgacg caaactcagt ccatcttcac
     1561 ctgcaacaaa aagaagagct tctattgagc ctaaataagt catttctctt cctttcttaa
     1621 gataacttga ctaaaattac tgtcttgttc tactgtgatt tgatgtaatt ttatcaactc
     1681 taacattgcc aaaaataaag ttatcatttc agattttgtt tggcaatccg caaacacttc
     1741 ttgtaatgtc gttttctttt taagattaaa atgtctttca ataacgatca tcatatcttc
     1801 aatacggtag tcttctttct caattaccgt ttgactattt gacaattctt tttgtttttg
     1861 ggacatcatt tgagaaaaag tcaaaaaaag atccatcacc gatttatcgt gtagaagaat
     1921 tgcatcttca aagataactt cttgttttgg ttttgaaaaa tatttggcgc gttcctgatg
     1981 ctgattagct aactcttcac ttagcacttt aaaacgtcta tattcctcta attggctcaa
     2041 caattccatt tctggatcat cttcaatagg atttgactcc actactttag gtaataagtt
     2101 acggcttttt atcaacatca gctgacttgc cataagcata tactcaccag ctacttctag
     2161 acgcatcgcc tgtaatgttg ctatataagc aaggtactgc tcaataactt ccacaatcgg
     2221 tacatcgtat atatcaacct catatttaga tacgaggtgc aagagtaagt ctaggggccc
     2281 ctcgaaatct tttaacttaa tatccattat ctataatatc tctctaaagt gataggagtt
     2341 tttaagccta gaaggcgact gagctccgtc atcgttttgc cattttcttt ttgctttaga
     2401 ataaattgtt ctcgcagaag ttgtgcagtt agttgttgct cattcttctc atttaagaaa
     2461 tctgttaatt gattaaaaaa ccattgtcta gaataaggct taccgttatg ctcaaagact
     2521 aagtcaccat cttcctctaa agattccaaa agaaaaggaa gcaagtcttc tggaagtttt
     2581 aaaatacgct tcatctgcga tttttcaata cttaagatat taaaagttgt atcaaaatca
     2641 gccttcttca aattagcaat ttcagccgga gttaaaccta aagataaaat caacaaagca
     2701 atcaaacgac cactcggata atcagtatct tgatagaaat ttgataaatc cattatttga
     2761 gactttgtct gagccaaagt gattttttca gtctcttgta gtctgtaaaa ttccttcagc
     2821 gttcctctat tatataaaaa gaaaagaaat tgattgactg cagataactt tcgcttacga
     2881 gctgttaact taaattcact tagagactgt tgatacaatg ccagtttttc agaatttaca
     2941 tgtccaccaa ttattttata aaattgcttt aaatcatagt gataggactt acgcgaattg
     3001 agagataatt tcttactctc aataaaatta ttaatatcat ttatcattgt ttttaggagt
     3061 gatagtatat tcgtctgtaa aatcatgtag gaggctatta atagctttta aaatcgattt
     3121 acgggttata atgcctctaa aatcattttg atcactgata acaggtaaaa atggataatc
     3181 tacaatctta tgcataattg ctgtcagagt agctgcctca ttaatcggct cgatttttgt
     3241 attaaccatc tcaacaatat cagtctgtgc catctcccaa tctgttaatt gacctttaga
     3301 ttggtagccc ataatgtctg agatactaat agtacctaca tattttttct ctttagtgat
     3361 aactggtaca cgtgaaaaac cattacttgc taatagtaac ataacgtggt ctgcattgtg
     3421 tgtatccaca aaaatagcaa cgtcttcagc tggaattaga tagtggtcca aatgatttag
     3481 caaaaacgac tcaaattctt tagcaatcat cttgaaaatt cctttgtcaa gttaggatag
     3541 ggttggtgtt ggcgagtata atactctacc aaaaccttac tatctgttat agttacaaca
     3601 gcgtaaagct tctcatttat tggtcctctt ggctgcaaaa cactacctgg attgataaaa
     3661 atagttttac catttttcca agcatctgca cgatgaagat gaccatataa acaaatatcc
     3721 gcgtcttctt gctgagctaa taaatccaat ttatcccagg taaaattaat ctgatggaga
     3781 tgaccatggg tttgaacaat aacagcatta tctatttttg taaccaaaac ttccggataa
     3841 cctgaatcat aatcacaatt tcctgtgacc acatgaatgc cttcccatat aggatctgag
     3901 ctaggaagtt cggaatcccc attgtgaaaa atagcatcca cttttcccaa ataatgattc
     3961 tttatatctt ttacaatgtc acgatctccg tgagaatcac tcattacaac taattttcta
     4021 attgccattt tggaaatacc tccatcaatt tacggacagc ttgcccacga tgtgatagat
     4081 tatttttttc ttgtgcgctc aattcagctg ctgtcctact accttctccg acaataaaga
     4141 gcggatcata accaaagcca ttttccccct taggttccgt accaatataa cctggccact
     4201 ctgcttcaac taccaaactt tctttatttg gagctgacac gactaaggtt gtatgaaact
     4261 gtgctgaacg tctttctttg tcaaatacca tggctaattc atgcaataat ttggcattat
     4321 tacgagcatc tgtcgcgtca ggacctgaaa aacgagcaga ccatacccct ggcaaaccac
     4381 ctaaagcatc tacttttaat ccggaatcat ctgaaattac catttttcca gttaattttg
     4441 aaatagtctc tgctttaagt cgagcatttt cttcaaaggt cataccagtc tcttcaactt
     4501 ctggtaaatc tgggtaatca tttaaatttt caactttgag tcctaatttc ccaaataatt
     4561 ctctaaattc cttcgttttt ccttcattgt gagtcgcaat taatatggta tccccaaaat
     4621 tattatcatt ctttaaatca aagaattttt ctaaagaagt gccctcttta ggtaagtgaa
     4681 ctaagaacaa ctgatcatct tcactaacta agatggcccc tttatgttga gtcactttga
     4741 tatgccttga atactcttga ttaatcatat caacgaggaa tgctaataat tgaaaatccg
     4801 aagtcatctg cacaacgtga agctgtagac cttcaacttt caaagttgat aatagacgat
     4861 gaaaaacatc ttggatagct tcgtaagatt catcattacc aaaacaagta aaataattaa
     4921 aagcttggaa actacctaaa aaccaattgc cttcagtttt actttcaaaa attgtttttg
     4981 tcataatacg atacgctcca catttatttc ttgactaagc cattgttctg caatttcttt
     5041 aaaacctttt gggctggcgg ttgtgtaaaa gtgatgacca ccgtgtttat tttgccaatt
     5101 atggtttatc tcaaaatagt tcaataaaac agaaatatca cgaacggttt ctgcgccact
     5161 atcaattaat ttaacctcag cccccataac attttgaatg atgggacgta ataggggata
     5221 atgcgtgcaa cctaaaatta aagtatctaa tttaccaact aatggggaca acgtttcata
     5281 aaccaccttt ttggctaaac tagaagacat ctgatttgat tccacaattg gaacaaattt
     5341 cggacaagca agggatacca cagcagtatt tggagacaaa gcttgaattt tttgacgata
     5401 agcatctgat ttaacagtca tgggagtacc tataatacca actttcccta aattagttga
     5461 tttgatagct gcgctagctc ctggtaaaat aacgcctaaa acagggatgt ctagtttttc
     5521 tttaatttct tgccaggcaa ctgcagttgc tgtattacaa gctataacaa tcatcttaac
     5581 atttttagtc aataagaagt taaccatctg ccaggtaaac tctctaatct gttgagcagg
     5641 tctaggacca tacggagctc tagcctgatc tccaatgaag attacttcct cttctggaag
     5701 ttgacggaac atttccttaa caaccgttaa accacctaca ccagaatcta gaaagccaat
     5761 aggcctacta tccattttta ttcctccaat attagtgact aaaagaggct ggacaacagt
     5821 cctagcctct ctatacaata accaatcagt caaatctaca cttgaccaag tttgcccaaa
     5881 tcttgtctag aaatctgatg gactatcact cactatttca tcactaaacg cttcaatgct
     5941 aaaacactct tattttttag ttttgatagc tgtttttgcg tgttttacaa tattacggta
     6001 tgtttgttga actttagctt cactaggttt ttgtcccatt tgactcatca tttcacgaat
     6061 agcatccggt gttaaacgag ggtgctctcc aatctctttt tcaatttgtt tacgagcaat
     6121 aaaaatgcct cccacgagac caccaaacaa agcgacgata attaataaaa tccaaatagt
     6181 tgttgacatc tattcattct cctcatttat ttacatgact cattatagca aaaaaagaaa
     6241 gagtttacaa atatatgtaa acaaaatcaa agcctctatc gtccttgtca cgtcaataca
     6301 ttattaaatt ttcactagag catggttaag cacatagtcg agtcctttat cttatcctaa
     6361 gtgttttccc caacttttgt gtcatccatt tccaaatgaa gagagctatg taaaaatcta
     6421 ccaatccatg tagcaaagag cctagtccta tcaaagaaaa tagtgaccat aaataagttg
     6481 aactactgtt agaagtcatt gtgattatat aaacagtaat aaattcgagc agaccatgaa
     6541 ttatgttaat agctacagca aataacaatg tttgccattt actcatcaat aaagagggct
     6601 ttgacttaat caagaaagcc gcaattaatg caaatccgat atgtgataaa gctctaagaa
     6661 cgattacaat cggaaaacca gctagtagaa atccaagtcc tgtccctaaa gctaccaata
     6721 ttgcaactgg aacagacata aaaatactta aaaataaagg tacatggctt gctaaggtga
     6781 aagaagcagg cccaataatt attttagcag gcatgattat aggaatcatg attgcaaaag
     6841 ctactaaaat agaagtataa gaaaccattt gtgttgtttt ttttgacatt tttatctcct
     6901 ttgaagctat tatacaaaat aaccttacag gtgtaaagac acctgtaagg ttatttgtct
     6961 atgtataaaa ttcctgcttt ttttagagcc tcacatattt ggtcaaaaac tgactgatct
     7021 tgacaagaaa tcatatgtgt atgtagacca tctgtcaaag aggatagcaa ttctgcattg
     7081 gaaactttta atttagcagt aaaattatca atgtcttttt ggctttgaat gtttaaggga
     7141 gcagtcagca tcccataaat agggtgctct acctccacat tcataatgat tccttgatac
     7201 cgcaaaataa tttccagttc ttcttctgtc tgttctatgc catgctgaca aaccaatcta
     7261 gctgtgaatt gatgagtact tagagcactg gacataaggt atccttttgg ggtagaaata
     7321 atatcacatt gttgtgcacg caataaggca atatctccaa caatcacctg cctactaaca
     7381 ctaaaaatct ttgctaatgt gcttgccgat atagcttctt ttgttccttt taaagtcgtt
     7441 aaaatatttt ctcgacgctc ctgtgccttc atataacaaa ttccttcttt ggtgttattt
     7501 cacaattaaa aactggctag aatatctaac cagttagcct caatcatttc gtgcaaaaag
     7561 acgaagaata tttaagaata gattaataaa atcaagataa aggcttagtg ccatcgaaac
     7621 tgcccaacca tctgcgactt ggccgccttg gctattgtag acatatttaa tcatttggtt
     7681 atcgtaagca atcaatccag aaaagatgat gacacatacg atactcatga tataactcat
     7741 acccccacta ccgataaata aattaacaag acttgcaatt aaaatcccaa ttaaagcagc
     7801 cattaacgct ttacgcatac ctgataaatc ttttttagtc ttagctccaa tcaaagccat
     7861 tgcaaagaaa acagccgcag atgttataaa agcttgaaga actgtagttt gcgtgtacct
     7921 tgcgataatg aaacttaaag taaacccatt caaagctgaa taaatcaaaa ataatggtaa
     7981 tgcagctggt gtgtttcgac gcgccgctcc actagcaaca aagactaaaa ccaattctaa
     8041 gatgattgct ccaaaataca accctggcat tttttgcatg atggcaatca tattttgtgg
     8101 aaacatatac agcataatcg cagaaacagc agctgacaaa cctactccta tccctacaag
     8161 accgtaaatc ttagcaaaaa attgattcag ccctgaatca gattgtgtgt aaatgacgtt
     8221 atcattcata tgtattttcc tattctaaat tttaatgatt atgactacct aagaatttgc
     8281 gactaaaacg gataggtttc ttaaaaatac gtttgagtgg acgagaagcc tctcgtactg
     8341 cccaatactt atcaaccata gtcacaatat aactttcttt ataacgtggc ggtgcaatag
     8401 tcgctcccca catgtgcttc aaaataatat cttcttctct agcgtttaaa tcagttaatt
     8461 ttcgcgcatt acgaaccgct atccttggat gaacccaggc atggctcttg ttaaatttcg
     8521 ttacacgcca atcataataa aagaaatcat gtagtaaacc acctcgagca gtactactcg
     8581 catcccaacc aaacttctta gcaattttat aacttgtata actaacattt atagaatgct
     8641 ctaagcgagt tgagtgatga tgttggacaa tagcttcgag tttttggaaa cgtggatggt
     8701 caataaggtg accgaccaaa tccatgaagt ccttatcgtc ttgatacttt ttcattataa
     8761 tcacccctct ggggatatta taacatatga acttaaatta agctgaaaat gactactccc
     8821 gcagctactg ctacattaag actttctgct tgtccaggca tttcaatatg tgctaaaaca
     8881 tctgcttctt ctgtcatagt tttggaaatt ccctgtccct catttcccat gattaaagca
     8941 aaataatcat atttagggag attcttataa tttaccgatt gttcagataa agttgtcgca
     9001 atcatagtta tcccttctgc tttacaaaag ttaacaaact gctctatttc taccctaaat
     9061 actggtaaat gaaagtgact gccttgcata gaacgaagtg ttttttggtt atacaaatca
     9121 gcagatttct gagataaaaa aacagcatca aaattcgctg catctgcagt tcgaaccatt
     9181 gttcctaggt ttcctggatc ctgcacatct tctaatacaa gaaaacgtcc ttttttaata
     9241 tctgtccatc ttgtctcttg aaaggcaatc tcagctacaa tcccttgagg agtctgtgag
     9301 tcagttaatt ctttcaaaac atcatctgta acaacaatag ctctctcggg ttttctcaaa
     9361 cgatctattg ccgtttctgt cacaaaaata tttaaaaatt gtgcaccata tttttcagct
     9421 tcctcaaata aatgccagcc ttcaataaga tatgaagatt tccgatattt tttttgtaac
     9481 aactttttcg tttttttaat gagattattt gattttgaag ttataatagt agtcataaac
     9541 ctattatatc agaaaagagg tcaagttatg aaaaaagttc atttaattgt ctcagggcgc
     9601 gttcaaggag tcggatttcg ctatgcaact tattccttag ctttagaaat aggtgatatt
     9661 tacggtcgcg tttggaataa cgatgatgga actgttgaaa ttttagcaca atccacagat
     9721 tcaaataaaa tgacacaatt tattcagaaa attcgaaaag gaccttcaaa atggtctaaa
     9781 gtaacttatg ttgatattaa actagataac ttcgatgatt ttaatgactt taaaatgagt
     9841 aattaaaaat cattctgcgg aagtattgtt taattcttaa aaaaaaagta aaatagatag
     9901 gtatattaaa aattagagga actaaaaaca ttgaaaaaaa cattgaaacg tatcttattc
     9961 tctagcttat ctttgtctat gttattatta ttgacaggat gtgttagcgt tgataaggct
    10021 ggaaaaccct atggagttat ctggaatact cttggagtgc ctatggccaa cctcattact
    10081 tatttcgctc aacaccaagg gcttggtttt ggtgtcgcta tcattattgt aaccgtcatc
    10141 gtacgtgtcg ttatcttacc acttggactt tatcagtctt ggaaagctag ctatcaagct
    10201 gaaaaaatgg cgtactttaa gcctttattt gaacctatta atgaacgact acgcaatgct
    10261 aaaacacaag aagaaaaatt agcagctcag acagaattaa tgacagctca acgtgagaat
    10321 ggccttagca tgtttggcgg tattggctgc ttgccattac tcattcagat gccatttttc
    10381 tcagctattt tctttgctgc acgctatact cctggagtct ctagtgcaac cttcttggga
    10441 cttaacttag gtcaaaagag tctgatactt actgtcatta tcgccattct ttattttgta
    10501 caatcttggt tgtcaatgca aggtgttcca gatgaacaac gtcagcaaat gaaaacaatg
    10561 atgtatctga tgccaataat gatggtattc atgtctatta gtttacctgc tagtgttgcg
    10621 ctttattggt ttattggtgg tattttcagt attatccagc agctagttac gacttatgta
    10681 cttaagccta aactacgccg aaaagtagaa gaagaatata ctaaaaatcc tcctaaagcc
    10741 tacaaagcta ataacgcacg aaaagatgtt acaaattcaa ctaaagccac tgaatctaat
    10801 caagcaatta ttacatctaa aaaaacaaat cgtaacgccg gaaaacaaaa aagacgggga
    10861 taaaacaagc atactaaata atggtgtgtt gtcctaaaca gttaaagatt attatgtaat
    10921 tcaagtaaaa aagacttagt tctatgtcct acatagctaa gtctttttag ttttgcctaa
    10981 cttttcatat aaaaagcacc ctaaaagtca gataaaaatc tagcttttaa agtgcaagta
    11041 atctagctaa cagactacct tattaataaa gtaatcgttt ttcccaccag tcttgaataa
    11101 ttgcataaag cgtacaaatt acccaataga taagggctac taagatatag gcggtcatat
    11161 agtcggagta ggttccccca actattttag cattattaaa gatatcaggt accgtaatca
    11221 tcgcagtcaa tgaggaaccc ttgaccatat ctagcaaaac attacttaaa gaagggagtg
    11281 caattcttgt tgcttgcgga ataataatgc ctctataaat cgtcggtgtt tttaacccta
    11341 aagctctagc agcttcccac tgaccatgat caacagctaa aagagctgct ctgataattt
    11401 ctgatatata tgcactagac atcatggtaa aacctacgat agaagcagtc acagcatcta
    11461 actgtatccc tataaaaggc aaaccaaagt agataagaaa gagtagaacc attaaaggaa
    11521 tcccacgcat taaggaaata tgaatattag ctagatacct cagtaagcgg aaatgagaca
    11581 tcttcattag agctacaaag aatcctacga tagctcctaa aataaagcca accaaggata
    11641 atccaatggt atatggtaac ccctgaataa cacttggaaa agccttaaca gctagttcca
    11701 aattaaaaat ggcatcccag tttatcactt atctactcct ctcgaaattt tttatttaac
    11761 tttaattact ggaattttat ccgttccagc aggttctttt gtgagatctt gaccagcata
    11821 atatttttca gagatttttt tcaaagaacc gtctttcttc atatccttga tagctgcatc
    11881 aatttttgtt ttgaggctct tatctttttt actcatcaca ataccttgct cagtcggatt
    11941 atacttcaca tcacccattt ttactttgat atctggatat tgttttgtta catattttac
    12001 agcaatgact tgtgtgtagt aatcattcgg aatgaaatct gtacgtccag ttgatacatc
    12061 tcgaaggtaa acgtcattag ttacattgtc ataaataaca ggttcagcac cttgctgttt
    12121 tgcaattttc atatattgcg ttccagcgcc accaccagct tttttacctt tccagtcaga
    12181 taaatctttt gctgtaatct ttgaagaacc atctgcacgt acaatcatac ctccaacaga
    12241 atatttataa ggttgagaga aattgtattt tttcaaacgc tcgggagttg tgtcaaagtt
    12301 attgacagca acatccacct taccactatc tacagaagta aatgattcag caactccaac
    12361 ttcaacaaac ttaactttta tcttaagttt tttagcaatc gctttcatca tatcaatctc
    12421 ataaccagtc aattttttat ggtcatcata ataacttgtt ggataaagcg ttgcaggggt
    12481 agcaacctta aggacaccat cttttttaat attgtcccat gttgtttctt tgttagattg
    12541 accatttgag caagctgcta atgtcattgc agataaaccg actaaaccaa gtaaaattgt
    12601 ctttttcata agtactcctg tagattaaat tatattaaaa tggtttggcg tactctatcg
    12661 aaaattaaat ttaatgaaaa ttaatacata aatttacgat aataatacaa ccattgttaa
    12721 gagtaccata ttgtatccgt tttcgcaaaa atattgttta cttttcaaat taatggtaaa
    12781 caggcccttt gaattgcttc tctttttcaa acaactctgc aacagcaagc aataaatctt
    12841 ctctcccttt agccgctgtc aattgtacac ctaaaggtag cccctcttta gttagatgag
    12901 ttggtatggc taaagaaggt tgccctgtca agtttggctg atgtgtaaaa ggtgtccagg
    12961 ccattgagtc ttcaaacatt ttccaaatta aatcctgttg ttcacttact gagaattctc
    13021 ccatatgcct caattgtttc tggagtgtct cgtctaaatc aaattgtcca tgaaaaggag
    13081 ctggttgatt agttgctgct gttaaaatta aatcataatt ttcatgaaac ctcgccatgg
    13141 tagctgcaaa ttgatcccat gaatctaaga gtttactgta atctttagcc aatacccttt
    13201 gtccactctg atacatagcc caggtcatta attccatatc gctaaattcc attgaacgcc
    13261 ctaaactctt ttcgatgtca tcaaacattg ctgctgtttc aacgctattc atcatacaat
    13321 aagttttcat agaatgaata ccatctaaag gttgttctac taattctaaa tgattccctt
    13381 tttgagacag aaaatctgcg gcttctttaa tagctgcttt agcctcactt gatacctttg
    13441 tttttaaggg agaatccatc aatactgcta tttttagggg tttactaacg ctaaactcga
    13501 aaagactctc tttgctgagt tttttcaggg gaaatgggct ttctacttga tatgattgta
    13561 aataatataa tagtcgtttt gtatctctaa ctgactttgt taaagcaaaa tgactagaag
    13621 ccccttgcca acctctatag gatgaaggtc caactggtat acgtcctcga ctcggtttta
    13681 aaccaatcaa accattaaaa cttgctggaa ttctgattga ccctcctcca tcactagctc
    13741 ctgcaattgg aaccattcct gaactaacag ctgcggctgc accaccacta gagcctccag
    13801 catttcttga atggtcaaat ggaagattaa cattcccgtg aagttgacca tcactaatat
    13861 tcttaaaacc aaattcaggg gtatttgttc ttcctaaaat aataaaccct aatttttcaa
    13921 aggactgcac taaatagtcc gtttgtttag catgatagtg tttaaataat tgtgaacctg
    13981 aggttgagag ttgtcctttt tgattctgtc ccaaatcttt cagaagaata ggaactcctg
    14041 caaaaggagc atttgattca tttgcgtatt tggctgcttg acgcgcctca ttatattgct
    14101 tgctgacaac agcattaacg cttacattct gttcctcgat cttgtagatt gcttgctcta
    14161 ctaattcttg cgacgaaatc ttatgctgtt ttatcgcttg taccatagcg gtagcatctt
    14221 taaaaaccat ataaacacct cataaaatgt atttttatta taagataaca aaaaatactt
    14281 aaaaaaaata gaataacgtc tagactctag atattattct atgatttata ctattaattt
    14341 gttttttcaa cactaatgat ttcaacttga tagctaccag caggtgattc aattgtagct
    14401 aagtctcctg tttttttacc aattaaagca tgagcaattg ggctttcatt cgaaattttt
    14461 cctgaaaaaa tgtcagctcc agcagcacca acgatatggt aagtatcttt atcatttgta
    14521 ccaacttctt ggacaaggac agttttacca attgcaacct catctttagc aactgcatca
    14581 ctatcaataa tttcagcata acgaattttt gtttctaaaa tttgaatttg accctcaaca
    14641 aatgcttgtt cgtcttttgc tgcatcatat tcgctatttt ctgataagtc accataagaa
    14701 cgagcaatct tgatacgctc tacaacttct ggtctacgaa ctaattttaa ctcttcaagt
    14761 tctttttcaa gttgatcttt ttccacttgt gtcattgggt aagttttttc tgccatttta
    14821 tttctctttt cttttcattt tttcaagtca aagatataaa catgatgact cgcaccatgt
    14881 ttataagctt agttaatttg actattgata tatttttcaa cgttagcctt gtgtgtttca
    14941 aaatctttag cgtagtatac atttccggtc ttcacatcag cgacaaaata aaggtaatct
    15001 gttgaagcag gttttatagt cgcttcaatt gctgagatac ttggactatc aactggtcca
    15061 ggcatcaaac cagtattagt atagatgttg tatggtgatt taattgaagt attaatttga
    15121 gcatcttcag ctaaacttgt tttatcacct aatttcccca tagcatacaa aattgcaatg
    15181 ttactttgaa gagcttgacc ggctgataat cggttataaa agacacttgc tattttacgg
    15241 cgatcttcgt ctgttgatcc ctctttttca acaagagatg ataatgtcaa aacatcattt
    15301 acagacatat tcttcgcttt aatagtgtta tagtaaggtg ctatcttagt attcattgtt
    15361 gaaatcatat cttcaacaag tccttctaag gttgtatctt tataatagtt gtaggtagct
    15421 gggaataaat atccttccaa ttgataaata gcttttgatt tatcaggtaa acttcctagt
    15481 aaatttggat atttagctac cattttttta ataaacgttt catcttgaac aagtttcaag
    15541 aaatcatcag ctttataagg tgtacttgta gtctttgtat caattttatt agactcaata
    15601 gctttagcaa tctgtttaat agtgtaaccc tcggtaatta atatcttacc taaagctggt
    15661 tttgtcggtt ctgcagttcc gcctttttcc aattcagcag caatctgatc taaagtcata
    15721 ctttttttaa gattgtagta tccactttga aaattactat agtttttgaa cttggaatag
    15781 taattaaaga ctgtggcgct cttaatgact cctgcttttt ctaaaatttg accaattaat
    15841 ttattcccag atcctatcgg aatttcaact tgtacaaatt tatttgaatt ggaatcaaca
    15901 ggtttaatag cactatctac aaaacgataa cctacaaatg ctgtcaagag caatacaagt
    15961 atgataattg atatcagaat acgtgcaatt cgttttgcca cattatctgc tttctgacgt
    16021 ctttcacgct tgttatgttt gttcttggct gacaaagcta taccctcttc ttcttttgac
    16081 aaatcctcta ctcggatagg ttcctttgct acgggctcta actctgatgt ctcaccaagt
    16141 tttacatttt caccttctgt aggaatatct gttgctctca caagaaagat ttcttccgtt
    16201 tcttgattag gcacagcagt tgcaacatca gtcttatctg cttttaaatc tatgtcaggt
    16261 acattttcag ttgtcctaaa ttcactagat ttggagccgc tatcaagctc tcttgaaggt
    16321 tccatccctt gattgataac ttgttcttct tgggaagcta atgcttgttc cgcctgacgt
    16381 ctttcttctt ctcgagcagc ttctgccaga agtttcgcct caatccgagc cttctcttgc
    16441 tcttctaagc gttgcttagt ctctagcgcc tttgctctag cttcttgttc gtctttcaaa
    16501 cgttgcgctt cataatctgc catcaattgt gctgttctgc gagcagcttc tttggcttct
    16561 tgctcttttt gatagagttc ttcttcacgc aactttctca aacggttagc ttcttctaac
    16621 tctgccagaa tttgttcttt aaaacttttc tgatcatggt tagaatgctg atcgtcatta
    16681 aattcggtca aagagatttc ctcctaataa taatcacagg caattatatc ttaaatagtc
    16741 tataaaatca acaaaacttt gacaatgtta tctactttta acaaattgat cgtgttggat
    16801 tattccaaat catattatac caaagcgaac caccatgttg ggagtcagat tctccctcat
    16861 ctttaaatcc attcatttca tagaaggaaa ttaaatcatc atggcaagtt aaacttatac
    16921 catctcgttc ttgagaaaca accaggtctt tcatcgctgc taataatgct gtaccaatcc
    16981 cctgaccttt aaaatcagga tgtattgata gactagtaat tcctatgaaa ccacctaccc
    17041 ttacagggaa ttcagaaact ttatggaata aatcgtccgt taagtaccga cctttaataa
    17101 ccggtccttc aatgtaacct gctaaacgac cattaatctc tgcaactaaa aaagtatcag
    17161 tcattatcgt taaacgttct tgcattgctt ttttactcgc agcctctgct tctgaaaaat
    17221 ttatttgttc aatactaatg acttgctgta aatcctctag gcaaccattt ctaataatca
    17281 tactcatctc cttaatttct ttatgaaatt ctcttgataa agtcagatag aggactgaga
    17341 aacttctctc agcccttacc atcaacttat tgtgtatttt tagttaggtt agctaataat
    17401 tcttcaaaag agcgctcata caattgaatg tctccagcac ccataaagac atagacagca
    17461 ttatcatgat tgagtaaagg cgagacattt tcgactgtca ctaaatctga gtgtttgaca
    17521 atcttagcag ctaaatcttc taccttcacc tcaccattat ctacttctct agcagaacca
    17581 tatatttgag cgagataaac gctatccgct tgactcaagg catgggcaaa ttcgtctaaa
    17641 agagctatcg tacgagtgaa cgtatgcggt tggaaaatag ctacaatttc ttttgacggg
    17701 tatttttgtc gagcagcatc taatgtcgca ataatctcag taggatggtg agcaaagtca
    17761 tcaataatga cagtatcgtc aataatcttc tcagtaaaac gacgctttac ccctgaaaat
    17821 gtcttcaaat gctcagctac taatgccata tcaattccca ttatgtaaag gttagcaata
    17881 acagcagttg catttaagat attatgttta ccgtatgctg gtacatgaaa ctgaccaatt
    17941 tcttcttggt tatagaaaac cttaaagtca gaaccattaa cagttcgagt gatgtctttt
    18001 gctataaaat catttgaatc ttcaaaacca taataatata ttggtgcctc agaagtgatt
    18061 tcatgaagtt ttggatcttc tccataaatg aataaacctt tttgaacttg cttagcatag
    18121 tcattaaagg cattgaatac gtcctctagg cctgtaaaat aatcaggatg gtcaaaatca
    18181 atattggtaa taattgagta ttctggatgg tacggcataa aatgacgttc gtattcatca
    18241 gcttcaaaca caaagtaatt agcattagca gaaccacgtc ctgtaccatc tccaattagg
    18301 aaagaagtgt ctgtaatatt ttttaaaaca tgagctaata aacctgtcgt tgaggttttt
    18361 ccatgtgccc cagctacacc tagactagtg aactgacgca taaaatctcc gagaaattca
    18421 tgatatcgtt taaaatgata gcccttttca ataacataag ccaactcttc attgttatct
    18481 ggacgaaaag catttcctgc aataatctct aaatcctcac tgatattatt cggtgagaaa
    18541 ggtaatatag ttacacctgc ttgctctaaa ccacgttggg taaaataata tttgtcaacg
    18601 tcacttcctt ggacgttatg tcccatttga tgaagcatca gtgctagggc actcattccg
    18661 gatcctttaa taccaataaa atgataagtt tttgacatgg tctctcctta attaatgtct
    18721 ttaaaacgtg tcagatttaa ttcttgagcc ttggctcttt ctttatgaaa ctgattgtct
    18781 tgtttattgt aaacttgact gcgtttcaaa aaatcaaaat tatttttctt tgtttttggg
    18841 ttcggtgtat tatcaggttc ttgaaatatg attggaattt ctgctaatat atagctttcc
    18901 tgctgtaaac gatcagaaag atgacttagt tcagtcacct ttcgctgagt ttgttttttc
    18961 ggtactgttg ccttcaattg ttgctgaaaa gcttcctttg atggcgcctt aaattcctgt
    19021 ttcgcatagt aagcttgtcg tttctcccga acagacctct ttgcctcttg tcgagatttg
    19081 ctcaactctg tcgctttttt tacaaaactc tgcggtggta caggatcatt ttcaattacc
    19141 ttaggatgtg atatttcttc atgttcataa ttgcttttga ccatgctttg atatgttttt
    19201 tcttgataaa agcctcgaat gttgttaatt aaatcttcat tatcataaag attcatcttt
    19261 acaagcggag acattactgg ttcatcatct gctatcaacg gaaatcgtct agagcctcta
    19321 ttcacattat cacttccttt ctacaacaga cctattataa actaattttt c
//
LOCUS       DATRQA010000026        19265 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.026, whole genome shotgun sequence.
ACCESSION   DATRQA010000026 DATRQA010000000
VERSION     DATRQA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19265
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.026"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            11..649
                     /locus_tag="VBD27_000671"
     CDS             11..649
                     /locus_tag="VBD27_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEO7024702.1"
                     /translation="MASILKKHLEKRLELKNDPMRTDMSTGASYPKYGFSVGKYTYGY
                     QQFFYEGVNLKEIGAFCSIAQNVTITGMNHPTDHITTNPFIYYKSRGFINEDRADLID
                     EKKNGKVIIGNDVWIGTNVTILPSVTIGNGAIIEAGSVITKDIPDYAVVAGTPAKIIK
                     YRFSEEEITLLNASQWWNWSDEAIKEHISEFSDKREFFNTLKSISENKNHKL"
     gene            complement(767..1870)
                     /locus_tag="VBD27_000672"
     CDS             complement(767..1870)
                     /locus_tag="VBD27_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7024703.1"
                     /translation="MSNIITYLKNNSNLTFDELALNDVDILCLNEFGYISFEKLINTT
                     EMKSVLVCELYHEYLQTMAKSYSFMFTSQRHDLCQLMMTSKRFKNLTLSYYQAEISLE
                     FEKQFAAMVFTIPNIDYHQVVFRGTDANLIGWKEDFKLTYMREISAHRSAIKYLNTIL
                     PYFDKVVLSGHSKGGNLALYAAMFTKPDLKAKIDLIWLIDSPGLQKTLLPTTEYKTTK
                     QKCIRLLPEESIVGMMLYSDIEPLIISSNARGILQHDVTTWEIQEPAILKTGAGLSLK
                     SICFEKTFQQWMAELKSQERKLFFDLLFDSFLSSGVSSLDDFNLASRAKMMKAFHSFR
                     ELDDDKKRLFNKSLKLLVTIFWGAYHDNSRETK"
     gene            complement(1870..4662)
                     /locus_tag="VBD27_000673"
     CDS             complement(1870..4662)
                     /locus_tag="VBD27_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEO7024704.1"
                     /translation="MASTNNVSDTNTRFAKEELEEVFEELGTTQGGLSDEEVAVRQKK
                     YGLNLLSEVKQESIILLFLKNFTSLMAILLWVGGFVAIVSNSLELGLAIWMVNVINGI
                     FSFIQEYRASQATQALKKMLPSYSRVLRKSSEEKVLSEQLVPGDIVLIEEGDCISADG
                     RLIKTTDLQVNQSALTGESNPIYKDSNVENYQSKTLIECDNMVFAGTTVSSGSATMVV
                     TAIGMQTQFGQIADLTQGMKSEKSPLQRELDRLTKQISIISITVGIIFFLAATFFVKE
                     PVSKSFIFALGMIVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSSVETLGATSVI
                     CSDKTGTLTQNEMTVNHLWQNGKSYQVTGLGYAPEGQILFEGDNICFGNSDRGDLEKL
                     IRFAHLCSNAQVLPPNDDRSTYTVLGDPTEACLNVLLEKSGINIQENRKFAPRLKELP
                     FDSVRKRMTTIHSLGGDEKDKKISITKGAPKEILDLSDYVLSDGKVIPLNKEERNKIQ
                     LANDTFAKDGLRVLAVSYCDIEGFSKEQWTQENLEQHMVFIGLIAMSDPPREGVREAI
                     DKCHAASIRIIMVTGDYGLTALSIAKNIGIIRNDDAKVISGLELSEMTDSQLKKELSG
                     EVVFARVAPEQKYRVVTILQEMGEVVAVTGDGVNDAPALKKSDIGVAMGVTGTDVAKE
                     SADMILTDDHFASIVHAVEEGRAVYQNIKKFLTYIFNSNTPEAVPSAFFLFSKGFIPL
                     PLTVMQILAVDLGTDMLPALGLGVEPPETDVMNRPPRRLTDRLLDKGLLIKSFLWYGT
                     IESVLAMGGFFWAHYLRYGNFTFFVANGIPYREATTMTLGAIIFSQIGMVMNSRTSYQ
                     SIKTLSIFGNKLINFGIIMEILAFLVLVYVPLFHNLFNTASLGLSHWLYLISCPFIVI
                     GLDEVRKLFSSRKNKR"
     gene            complement(4811..5248)
                     /gene="msrB"
                     /locus_tag="VBD27_000674"
     CDS             complement(4811..5248)
                     /gene="msrB"
                     /locus_tag="VBD27_000674"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO7024705.1"
                     /translation="MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVD
                     IVSGEVLFSSLDKFQSGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGH
                     VFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFDE"
     gene            complement(5248..5712)
                     /locus_tag="VBD27_000675"
     CDS             complement(5248..5712)
                     /locus_tag="VBD27_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7024706.1"
                     /translation="MIRRAKEKDLPAIAELLKQILVLHHGVRPDIFHTRGSKFSKEQL
                     KEMLMDESRPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQ
                     IGQKLMDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL"
     gene            complement(5721..6380)
                     /locus_tag="VBD27_000676"
     CDS             complement(5721..6380)
                     /locus_tag="VBD27_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7024707.1"
                     /translation="MTMVKREDVILNATENMVYHKLKNDPSGHDWFHIVRVRNLAVEL
                     AHKEGANTFICQMAALLHDIIDDKICQDSKQASYELTQWLYSQDLAIAEVEHILDILE
                     NISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPREN
                     LTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGK
                     G"
     gene            complement(6480..9113)
                     /locus_tag="VBD27_000677"
     CDS             complement(6480..9113)
                     /locus_tag="VBD27_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7024708.1"
                     /translation="MKGQKIIALAGLVLSCHFALTACHTQEHKNSYHIKTKQVAKKKA
                     NKKKVSVKESHKKRKGVAGVDFPTDDGFLLTKDSKILSHTDSGIVVAHGNHSHFIFYS
                     DLKGSKFSYLIPNANAKTNKNQAVRNFKAGAVAVNTLNDGYVFNPADIVSEDANGYVV
                     RHGDHFHYIPKASLSQQKQVQASRAVSRLGNQSNSHYRVNSSKIAGLHYPTSDGFLFN
                     GRGIKGTTPTGILVEHHNHLHFISFADLRKGGWGSIADRYQPQKKADSKKQSPSSKKP
                     RNENTLPKDIKDKLAYLARELHLDISRIRVLKTLNGEIGFEYPHDDHTHVIMAKDIDL
                     SKPIPNPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDENQKKLIYLAEKLGLNPN
                     QIEVLTSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQI
                     GFDDEIIQDILHADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPN
                     IEVLGIGFTPINDMTPVLQFKKLKQLWMTNTGITDYSFLDKMPLLEGLDISQNGIKDL
                     SFLTKYKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNLTKLQELHL
                     DHNNVKNLSALSGKKDLKVLDLSNNKSADLSTLKTTSLETLLLNETNTSNLSFLKQNP
                     KVSNLTINNAKLSSLDGIEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTS
                     LEGVNNYTSLETLSVSRNKLKSLDIKTPNKTVTNLDFSHNNVPTSQLKLNEKNIPEAV
                     AKNFPAVVEGSMVGNGSLAEKAAMASKEDKQVSDNTNHQKNTEKSAQANADSKKENPK
                     THDEHHDHEETDHAHVGHHHH"
     gene            complement(9359..11191)
                     /gene="lepA"
                     /locus_tag="VBD27_000678"
     CDS             complement(9359..11191)
                     /gene="lepA"
                     /locus_tag="VBD27_000678"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO7024709.1"
                     /translation="MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGFTIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGMVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVAD
                     TRVGDTITLANNPAIDPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLE
                     VSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK"
     gene            complement(11327..11743)
                     /gene="ndk"
                     /locus_tag="VBD27_000679"
     CDS             complement(11327..11743)
                     /gene="ndk"
                     /locus_tag="VBD27_000679"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO7024710.1"
                     /translation="MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRHADADI
                     LKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIR
                     GDFAQAPSPNQATCNIVHGSDSPESAAREIAIWFNN"
     gene            complement(12053..12313)
                     /locus_tag="VBD27_000680"
     CDS             complement(12053..12313)
                     /locus_tag="VBD27_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024711.1"
                     /translation="MLGHRCGVLVFCYTEINFQRMWGKAVIRLIQDVIQNLVGQANEI
                     TPVYQFDWETYILATKKYERHLEVCLLVENSNYFSDSKRMCR"
     repeat_region   12366..13193
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=12366..12401
                     /rpt_unit_seq="gttttggaaccattcgaaacagcacagctctaaaac"
     gene            complement(13294..13959)
                     /gene="csn2"
                     /locus_tag="VBD27_000681"
     CDS             complement(13294..13959)
                     /gene="csn2"
                     /locus_tag="VBD27_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEO7024712.1"
                     /translation="MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEH
                     LKLFDDKQKSLKATELMMVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLA
                     ATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLT
                     KKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGE
                     NMV"
     gene            complement(13946..14287)
                     /gene="cas2"
                     /locus_tag="VBD27_000682"
     CDS             complement(13946..14287)
                     /gene="cas2"
                     /locus_tag="VBD27_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO7024713.1"
                     /translation="MSYRYMRMILMFDMPTETVEERKAYRKFRKFLLSEGFIMHQFSV
                     YSKLLLNNTANNAMIGRLKVNNPKKGNITLLTVTEKQFARMVYLHGERNTSVANFDSR
                     LVFLGDSYDQD"
     gene            complement(14284..15153)
                     /gene="cas1"
                     /locus_tag="VBD27_000683"
     CDS             complement(14284..15153)
                     /gene="cas1"
                     /locus_tag="VBD27_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO7024714.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLVFKDSYQTEMIHLSEIDILIME
                     TTDIVLSTMLIKRLVDENILVIFCDDKRLPTAMLMPYYARYDSSLQLSRQMSWIEDVK
                     ADVWTSIIAQKILNQSFYLGECSFFEKSQSIMNLYHDLESFDTSNREGHAARIYFNTL
                     FGNDFSREHDNPINAGLDYGYSLLLSMFAREVVKCGCMTQFGLKHANQFNQFNLASDI
                     MEPFRPIVDRIIYENRQSDFVKMKRELFSMFSETYSYDGKEMYLSNIVSDYTKKVIKS
                     LNSDGNGIPEFRI"
     gene            complement(15155..19249)
                     /gene="cas9"
                     /locus_tag="VBD27_000684"
     CDS             complement(15155..19249)
                     /gene="cas9"
                     /locus_tag="VBD27_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286032.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEO7024715.1"
                     /translation="MGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIKKNLIGA
                     LLFDGGNTASDRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLEDSFLVE
                     DDKRGSKYPIFATMQEEKDYHEKFPTIYHLRKELADKKEKADLRLVYLALAHIIKFRG
                     HFLIEDDSFDVRNTDIQRQYQAFLEIFDTTFENNHLLSQNIDVEGILTDKISKSAKKD
                     RILAQYPNQKSTGIFAEFLKLIVGNQADFKKHFNLEDKTPLQFAKDSYDEDLENLLGQ
                     IGDEFADLFSVAKKLYDSVLLSGILTVTDLSTKAPLSASMIQRYDEHREDLKQLKQFV
                     KASLPEKYQEIFTDSSKDGYAGYIEGKTNQGAFYKYLSKLLTKQEGSEYFLEKIKNED
                     FLRKQRTFDNGSIPHQVHLTELKAIIRRQSEYYPFLKENLDRIEKILTFRIPYYVGPL
                     AREKSDFAWMTRKTDDSIRPWNFEELVDKEASAEAFIHRMTNNDLYLPEEKVLPKHSL
                     IYEKFTVYNELTKVRYKNEQGETYFFDSNIKQEIFDGVFKEHRKVSKKKLLDFLAKEY
                     EEFRIVDVIGLDKENKAFNASLGTYHDLKKILDKDFLDNPDNESILEDIVQTLTLFED
                     REMIKKRLENYKDLFTESQLKKLYRRHYTGWGRLSAKLINGIRDKESQKTILDYLISD
                     GRANRNFMQLIHDDGLSFKPIIDKAQAGSHSDNLKEVVGELAGSPAIKKGILQSLKIV
                     DELVKVMGYEPEQVVVEMARENQTTNQGRRNTRQRYKLLEEGVKNLASDLNGNILKEY
                     PTDNQALQNERLFLYYLQNGRDMYTGEALDIDNLSQYDIDHIVPQAFIKDDSIDNRVL
                     VSSAKNRGKSDNVPSIDIVKARKAFWKKLLDAKLMSQRKYDNLTKAERGGLTSDDKAR
                     FIQRQLVETRQITKHVARILDERFNNELDSKGRRIRKVKIVTLKSNLVSNFRKEFGFY
                     KIREVNDYHHAHDAYLNAVVAKAILTKYPQLEPEFVYGDYPKYNSYKTRKSATEKLFF
                     YSNIMNFFKTKVTLADGTVVVKDDIEVNNDTGEIVWDKKKHFATVRKVLSYPQVNIVK
                     KTEIQTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPIVAYSVLVVADIKK
                     GKAQKLKTVTELLGITIMERFRFEKNPSAFLESKGYLNIRDDKLMILPKYSLFELENG
                     RRRLLASAGELQKGNELALPTQYMKFLYLASRYNELKGKPEEIEQKQEFVVQHVSYFD
                     DILQIINDFSNRVILADANLEKINKLYQDNKENISVDELANNIINLFTFTSLGAPAAF
                     KFFDKIVDRKRYTSTKEVLNSTLIHQSITGLYETRIDLGKLGED"
BASE COUNT         6036 a         3733 c         2994 g         6502 t
ORIGIN      
        1 gaggctagtt atggctagta ttttaaaaaa acatttagag aaacgtttag agcttaaaaa
       61 tgatcctatg aggacagata tgagtactgg tgccagttac cctaaatatg gattctcggt
      121 aggaaagtac acatatggat atcaacaatt cttttacgaa ggcgttaact taaaagaaat
      181 tggagcattt tgctcaatag cccagaacgt tactattact ggtatgaatc atccaaccga
      241 tcacattacc actaaccctt ttatttacta taagtcaagg ggatttataa atgaggatag
      301 ggcggactta atagatgaaa agaaaaatgg aaaagttatt attggcaacg atgtctggat
      361 aggaactaat gtcactatac ttccatctgt tacaataggt aatggagcaa ttattgaagc
      421 aggttcagtt attaccaaag acattcctga ctatgctgtt gtcgctggaa cacctgctaa
      481 aattatcaaa tatcgctttt cagaggaaga aataacatta ttaaatgcat ctcagtggtg
      541 gaattggtca gatgaagcta ttaaggaaca tattagcgaa ttttcagata agagggaatt
      601 cttcaatact ctaaaaagta tttctgaaaa caaaaatcat aaactctaga atgacttcat
      661 caaatagcac actaaaatac tactcagcat aatatgagta gtattttagt atgctatttt
      721 gctagtttat ttagcacatg tttagactaa tgcaattcta ggaaagctac ttagtctctc
      781 tgctattatc atgataagct ccccaaaata tggttactaa taattttaaa gatttgttaa
      841 ataacctttt tttatcatca tctagttctc taaagctatg aaaagctttc atcatttttg
      901 ctctggaagc caagttgaag tcatccaaac tagatacgcc acttgatagg aagctatcaa
      961 ataataaatc aaaaaataat tttctttctt gactttttaa ttcagccatc cactgttgaa
     1021 atgtcttttc aaaacaaata ctttttagcg atagtcctgc tccagtcttt aaaatagctg
     1081 gttcttgaat ttcccaagtt gtaacatcgt gttgcagaat tccacgcgca ttgctactaa
     1141 taattagcgg ctctatatca ctataaagca tcataccaac aatagattct tctggtagta
     1201 agcgaatgca cttttgttta gtagttttat actctgtagt aggtaacaaa gttttttgta
     1261 gtcctgggga gtcaataagc caaatcaaat caatttttgc ttttaaatct ggctttgtaa
     1321 acattgcagc gtacaaggct aaattaccac ccttagaatg acctgagaga actactttat
     1381 caaaataagg cagtattgta tttaagtatt taatcgctga acgatgagcc gatatttctc
     1441 tcatatacgt caatttaaaa tcttctttcc aaccaataag gttggcatca gtaccacgaa
     1501 atacaacctg atgataatca atattaggaa tagtaaaaac catagctgca aattgttttt
     1561 caaattctaa actaatctca gcttgataat aagataaagt taagttttta aaacgtttcg
     1621 aggtcatcat taactgacat aaatcatgtc gttgtgacgt gaacataaag ctatatgatt
     1681 tagccatagt ttgaaggtac tcatgatata actcacagac taaaactgac ttcatttctg
     1741 tagtgtttat aagtttttca aaagaaatat agccaaattc atttaaacaa agaatgtcaa
     1801 catcgtttaa tgctaattca tcaaaagtta agtttgaatt attttttaaa taagttatta
     1861 tatttgacat caccgtttat tctttctact gctgaataat ttccgaactt catctaaacc
     1921 tatcacgata aacgggcagc ttattaaata gagccaatgt gataaaccta ggcttgctgt
     1981 gttgaataag ttatggaata gtggcacgta tactaagact aaaaatgcta aaatctccat
     2041 tattatacca aaattaatta atttattacc aaaaatagat agtgttttaa tagattgata
     2101 gctcgttctg ctattcataa ccatacctat ctggctaaag ataatagctc caagtgtcat
     2161 agttgtcgct tctctgtacg gtatgccgtt tgcgacgaaa aaagtaaaat taccataacg
     2221 taagtaatga gcccagaaaa aaccacccat agctaatacg gattcaatag tgccgtacca
     2281 aaggaaagat tttataagta gccctttgtc caataagcga tctgttagcc gtcttggagg
     2341 acgattcata acatctgttt ctggtggttc cacgcctaga cctaaagcgg gaagcatatc
     2401 ggttcccaaa tcaacagcaa gtatttgcat tacagtcaat ggtaaaggaa taaaaccttt
     2461 agaaaaaagg aagaatgccg acggaaccgc ctcaggagta ttactattaa aaatatacgt
     2521 caagaatttc ttaatgtttt gataaacagc ccgcccttct tccacggcat gtacaataga
     2581 agcaaaatga tcatctgtca gaatcatatc agctgattct ttagcaacat cagtaccggt
     2641 aacacccatg gctacaccaa tatcggattt ttttagcgca ggcgcatcat tgaccccgtc
     2701 tcctgtaact gctactactt cacccatctc ctgtaatatc gttactactc gatatttttg
     2761 ttctggtgct acacgagcaa aaacgacttc acctgacaat tcttttttca attggctatc
     2821 cgtcatttct gataactcta aacctgaaat tactttagca tcatcgttac gaataatacc
     2881 aatattttta gcgatactca gtgccgttaa tccgtaatct ccagtcacca taataatacg
     2941 aatgcttgct gcatgacatt tatcaatagc ttcacgaacg ccttcacgag gtggatcact
     3001 cattgctatc aaccctataa acaccatgtg ttgttccaaa ttttcttgcg tccattgttc
     3061 ttttgaaaag ccttcaatat cgcagtatga aacagctaag acgcgaagtc catctttagc
     3121 aaatgtatca ttagccaact gtattttatt acgttcttcc ttatttaagg ggataacttt
     3181 gccatcagat aagacataat ctgataaatc aagtatttct ttcggcgcac cttttgtgat
     3241 tgaaattttt ttatcctttt catcgcctcc taagctatga atagttgtca tgcgttttct
     3301 aaccgaatca aaaggtaact ctttcaaacg tggtgcaaac ttacgatttt cttgtatgtt
     3361 tatccctgat ttttccaata aaacattcaa acaagcttct gtcggatctc caagtacagt
     3421 ataagttgaa cgatcatcat ttggtggtaa tacttgagcg ttgctacaga gatgagcaaa
     3481 acgaataagt ttttctaagt ctcctctatc actattacca aagcaaatat tgtcaccttc
     3541 aaaaagaatc tgtccttctg gagcataacc cagtcctgtc acttggtaac tctttccatt
     3601 ttgccacaaa tgattgacgg tcatttcatt ttgcgttaat gtaccagtct tatcagaaca
     3661 gataactgag gttgctccaa gagtctcaac tgaagaaagt tttttgacaa gtgcgtgttc
     3721 tttagccatt cgttggactg ccatagctag agaaagtgta acggtaggta gtaaaccttc
     3781 tgggataaat gcaacgatca tccccaaggc aaagataaat gattttgaca ctggctcctt
     3841 aacaaaaaaa gtcgctgcta agaaaaagat aattcctaca gtaattgaaa tgatagaaat
     3901 ctgtttggtc aaccgatcaa gttctctttg aagagggctt ttttctgatt tcatcccttg
     3961 tgttaaatca gcgatttgac caaattgggt ttgcattcca attgccgtaa caaccattgt
     4021 tgcgctacca ctggagactg tcgttcctgc gaaaaccata ttatcacatt caatcaatgt
     4081 tttagactgg tagttttcaa cattgctatc tttataaata ggatttgatt cccctgtcaa
     4141 agctgattgg tttacttgca aatctgttgt tttaatcaag cgaccatctg ctgaaataca
     4201 atctccttct tcaataagaa caatatctcc tgggactagt tgctctgata ataccttttc
     4261 ttctgagctt tttctaagaa cacgtgaata agatggtaac attttcttga gagcttgtgt
     4321 tgcttggctt gcacggtatt cctgaatgaa actaaaaata ccattaatca cattaaccat
     4381 ccaaatggca agacctagct ctaagctatt agaaacaata gccacaaatc ctccaaccca
     4441 taatagtatt gccataagac tagtaaaatt tttcagaaac aataaaataa ttgattcttg
     4501 ctttacttca gataacaggt ttaaaccata ttttttctga cgtacagcaa cctcttcatc
     4561 tgataaacca ccttgagttg tccctaattc ttcgaaaacc tcttctaatt cctctttagc
     4621 aaaacgtgtg tttgtatctg aaacattatt tgtagaagcc atattctagt ctccttctct
     4681 aaaacgtttt catttttgat acctatatta tactcttttt tcagaaaata aactgatgaa
     4741 gaatacacct gccaccaatt ctttttacca tacataaaaa acgacttaag atataccctt
     4801 aagtcgtctc ttattcatca aaaagtgata agtagtcccc atagcctctc tttgccatct
     4861 ggtcataagg aataaaatct aaggcagctg aattgataca atagcgtaag ccacctgcat
     4921 caaccggtcc atcattaaat acatgcccta aatgagagtc agcttgacga ctcctaacct
     4981 caatacgatg cattccatga gagtgatctt gatgatttgt caccattcga ttctcaattg
     5041 gttttgaaaa tgctggccaa ccacaaccag attgaaattt atctaaagaa gagaataaaa
     5101 cctctccact gacgatatct acatatatcc cctcttcaaa aaaatcatca tacttcccag
     5161 taaaagcatg ttccgtagct gaattttggg ttacttgata agctgtatgt ccgatacgct
     5221 gacgtaattc ttcttgtgtt tctttcatta tagtatctcc tccatttgag tttgttgagg
     5281 tttaaaacct aatttttcat aaaaagagac cgctctctga ttatcattcc aaacatttaa
     5341 agttatatta taacacccat gtttcttagc atattgtctt gcaaaatcca tcaacttttg
     5401 tccaatttgc tgacctctta cttcctcatc aatgcacaaa tcatctatat agagagtttt
     5461 aaaggacttt cgaggtaagt cacgtttttc ttgtaattga agaaacaagt gggcaacaac
     5521 tttaccctcg tctgattcat aaacaaagat aggccttgat tcgtccatca gcatttcttt
     5581 caattgttct ttagaaaact tacttcctct tgtatgaaaa atatctgggc gaaccccatg
     5641 atgtaacact aaaatttgtt tcaatagctc tgcaatagca ggcaaatctt tttctttcgc
     5701 tctacgaatc atatctactc ctaaccttta ccattccatt cagcataaaa ttcggctaaa
     5761 tatagttcta aaaaatcatg acgtttttga gcaagcattt tcccttgttt tgtattcata
     5821 aggtctttta actttaataa tttttcgtaa aaatgcatga tagctgtatc ctgaccatta
     5881 cgatactctt ctaaagtgag gttttctcgt ggttttaagt tagggtcatg aattaaacgt
     5941 cctttactcc ctgaataagc catcgttctt gcaataccaa tagcccccat agcatctagg
     6001 cgatctgcat cttgaacaat ttgaccttcc aatgttttca tagtcaatcc agttcctgct
     6061 ttgaatgaaa tattttcaag aatatcaagg atatgttcta cttctgctat ggcaagatct
     6121 tgactatata accattgagt caattcatac gacgcttgct tactatcttg gcagattttg
     6181 tcatcaataa tatcatgcaa aagtgcagcc atttgacaaa taaaggtatt agcaccttct
     6241 ttgtgtgcta attctactgc aagatttctt actctaacaa tatgaaacca gtcatggcca
     6301 cttggatcat tttttaattt atggtaaacc atattttcag ttgcatttaa aatcacatct
     6361 tctcttttaa ccatagtcat attatagcta attttctaaa aaaataaatg atttagcata
     6421 taaatgcaaa atcctccaag aaattcttga aggattttat atattagctc cgtaaaagat
     6481 taatggtgat gatgacctac atgtgcatga tctgtttctt catggtcatg atgttcatca
     6541 tgtgttttag ggttttcttt tttactatca gcattcgctt gagcagattt ttcagtgttt
     6601 ttttggtgat tagtattatc tgaaacttgt ttgtcctctt tagaagccat agctgctttt
     6661 tcagcaagac taccatttcc aaccattgaa ccttctacaa ctgctggaaa attcttagct
     6721 actgcttcag gaatattttt ttcattcaat ttcaattgtg atgttggaac attgttatgg
     6781 ctaaaatcaa gatttgtaac tgttttgtta ggtgttttaa tatctaaaga tttaagttta
     6841 tttctagaaa cacttaaggt ctcaagtgaa gtataattat taacaccttc aagtgatgtt
     6901 aactgattat tagtcacatt caagaatttt aacgaacctt gtttattttt caatactagt
     6961 gacttaattt ggttcccttc agcttctact ttaacaattt catcgctctc ttcaatacca
     7021 tctaatgatg ataatttcgc attattaatt gttaaattag ataccttagg attttgcttt
     7081 aagaaactta gattgcttgt attcgtctca tttaaaagaa gcgtctcaag actagttgtt
     7141 tttagagtcg ataagtctgc ggatttattg tttgataaat ctagaacttt caaatctttt
     7201 ttaccagaaa gtgcacttaa attcttcaca ttattatgat caaggtgcaa ttcttggagt
     7261 tttgtcaagt ttgataacgg tgtcaagtca gagatattgt tatgactcaa aactaagaat
     7321 tgaaggtttg gtaattcagc caatggtttt agcgaagtaa tgccattatt agctgcagca
     7381 ataagactta attgtttata ttttgtaagg aaagataaat ccttaatacc gttttgtgaa
     7441 atatccaatc cttctaatag tggcatctta tcaaggaaag aataatctgt aataccagta
     7501 tttgtcatcc aaagctgttt aagttttttg aattgcaaaa ctggcgtcat atcattaatc
     7561 ggagtaaacc caattcctag aacttcaata ttaggcatca atgatagacc aaaacgctgg
     7621 aatggattgg ttctttgtcc aatgttaatc ttagtaacag ttgcaagcca ctgacgcatt
     7681 ttttcaggat tagtttcatt tgatgggaat ggtgttggag catctgcatg cagaatatcc
     7741 tgaataatct catcatcaaa accaatttgt tttagagttg ccataccaac tttatctttt
     7801 gcatgagagt gatcgtggtg tccatctggg ataggcttcc caatttcgat atccttactt
     7861 gctatagtgt gtgagtggtc atcatgtgga tacttaaaga tgatacttcc atcctctgac
     7921 gttaatactt caatttgatt aggatttaaa ccaagcttct ctgctaaata aattaatttc
     7981 ttttgatttt catcagataa tttatttgat ttagtgtgct cgtgttcttc atgcttatgg
     8041 tcggactcat catggtgatg acctttgtga tgatcttcgt catcatgatg tggatttgga
     8101 attggcttac ttagatcaat atctttggcc ataataacat gtgtatgatc atcatgtggg
     8161 tattcaaatc caatttcacc atttagtgtt ttaaggactc tgatacgtga aatatctaaa
     8221 tgtaattcac gagcaaggta agctagctta tctttgatgt ccttaggtaa agtattttca
     8281 tttcttggct ttttacttga tggtgactgt tttttagaat cagctttctt ttgtggttgg
     8341 taacggtcag caattgatcc ccacccccct tttcttaaat cagcaaaact aataaaatgc
     8401 agatgattat gatgttctac taaaatacct gttggggtag tgccttttat tcctcgtcca
     8461 ttaaataaga aaccatcact tgttgggtag tgaagccctg caattttaga actattaacc
     8521 ctgtaatggc tattactttg atttcctaaa cgtgaaacag ctctacttgc ttgtacttgc
     8581 ttttgctgag ataaacttgc ttttggaata tagtggaaat ggtcaccatg tctgaccacg
     8641 tagccattag catcttctga aacaatatct gcgggattaa aaacatagcc atcatttaat
     8701 gtattaacag ctaccgcccc tgctttaaag tttcttaccg cttgattttt attagttttt
     8761 gcattagcat tagggattaa atagctaaat tttgaacctt ttaaatcaga gtaaaaaata
     8821 aaatgagaat gatttccatg tgctacaacg attccagaat ctgtatgaga taaaatcttc
     8881 gaatctttag tcagtaaaaa gccatcatca gttggaaagt caacccctgc aacacccttg
     8941 cgttttttat gactttcctt aacagagact ttctttttat tagctttttt cttagcaacc
     9001 tgctttgttt taatatgata agaatttttg tgttcttgag tatgacatgc cgtaagagca
     9061 aaatggcatg acaggacaag accagctaga gcaataattt tttgaccttt catgagtcct
     9121 ccataaatgt tttttcgttt tagttaacta gttaattata tcagatgttt ttctttttgc
     9181 aatactattt gccatctttt tattatttat ttgctaacaa aaactcattt atagctcatt
     9241 tatatttaaa taaagatatt ataaagccat attatttgat aattgcagac aaagaaaaag
     9301 taacttaagc acgaagctaa gttactttaa aatagataaa tgatatcatc tttctgaatt
     9361 attttttgtc atcatcgtcc attgataaaa cacttaagaa agcttcctga ggaacttcga
     9421 cggaaccaat tgctttcata cgctttttac cagctttttg tttttcaagc aacttacgtt
     9481 tacgtgaaac gtcaccacca taacattttg ctaagacatt tttgcgtagt gcttttatat
     9541 cagaacgagc cacaattttt tgtccaatag ctgcttggat aggaacttca aactgctgac
     9601 gaggaatgat ttttttcaac ttatcaacga tcaatttacc acgttcatag gcaaattctt
     9661 tgtgaacaat aaagcttaaa gcatccactt tatcaccatt taaaagaata tccattttaa
     9721 ctaactgtga gcgacgatat tcggaaattt cataatcaaa actagcatag ccacgtgttg
     9781 atgatttaag cttatcaaag aagtcaaaca caatttcagc taaaggaatt tggtaaataa
     9841 cattaacacg attatcatca atataatcca ttgttacaaa gtcaccacgt ttacgttgtg
     9901 ctaactccat tacagcaccg acaaattctt gaggaaccat aatttgcgcc ttaacatatg
     9961 gttcttcaat gctgtcaaca cgagtaggat caggaaattc agatggattc gaaacttcaa
    10021 gcatttcacc atcagttgta ttaacatgat aaactactga tggtgcagtc ataataagat
    10081 caatattgaa ctctcgttcc aagcgctctt ggataacgtc catatgtaaa agtcctaaga
    10141 aaccacaacg gaaaccaaac cctaatgctt gtgatgtttc cggttcaaac tgtagactag
    10201 catcattcaa ttgtaatttt tcaagggctt cacgcagatc gttatactta tttgattcaa
    10261 ttggataaag accggcaaaa accattgggt tcatttgctt atagccatgc aaggggtcta
    10321 tagctgggtt attagcaagg gtaatggtat caccaacacg tgtatcagca actgttttaa
    10381 ttgaggcagc aatataccct acatctccag ttgctaaaaa atcacgtcca actgctttgg
    10441 gagtgaaaat accaacctca gtaacgtcga aggttttacc attagacatc atttgaatct
    10501 tatctcctgg cttaaccata ccatttacaa tccttacttg cagaattact ccacgataag
    10561 catcgtaaac agaatcaaaa attagagctt gaagaggagc atctacttcc ccagtcggag
    10621 caggaacttt ttcaacgatc tgctctaaaa tttcttcaat accaatccct gccttagctg
    10681 aggcaagtac agcttctgaa gcatctagac caataacatc ctctacttct gcacgtacac
    10741 gttctggatc tgctgctggt aaatcaattt tattaataac tggtagtatc tctaaatcat
    10801 tgtctagagc caaataaaca tttgccagag tttgagcttc aattccttgg gctgcatcaa
    10861 caaccagtat agcaccctca caagctgcca atgaacgtga tacttcatat gtaaagtcaa
    10921 catgccctgg cgtgtcaatc aagtgaaaaa tgtaagtttc accatcttta gcagtataat
    10981 taagctcaat agcatttaac tttatagtaa acccacgctc acgttctagg tccatagagt
    11041 ccaacagttg cgcctgcatt tcacgactag aaaccgtttc tgttttctct aaaatacggt
    11101 ctgctaaggt tgattttcca tggtcaatat gagcgataat cgaaaagttt ctaatctttt
    11161 cttgacgttt cttcaaatct tcaatattca ttctatttcc tatacaattt gatatgttta
    11221 acttctaaaa tattaaaaca gtcctataga ctctagaata ttatatcaaa aagaagaccg
    11281 atgtaaaaga gaattaacac ttgaacatca atcttcatta agtctattag ttattgaacc
    11341 aaatggctat ctcgcgagct gcagattcag gtgagtctga cccatgaaca atattacatg
    11401 tcgcttggtt tggagaggga gcttgtgcaa aatctcctcg aatagttcct ggaagggcat
    11461 ctttggggtt agtactcccc atcatggtgc gccaagttga aatgacctct tctcccgaaa
    11521 taactccaat gattactgga ccactagtca tataatctac taatgttgga aagaatggtc
    11581 tatccgtcag ttctgcataa tgtcttttca aaatatctgc atctgcatgt ctaacctcta
    11641 agcgatcaat agagaagcct cgacgctcaa tacgactaat aacttctcca ataaaaccac
    11701 gttttacacc atcaggtttt atcataaaaa aggtttgttc cataatatta gcacctcatt
    11761 tctttctcca ctattataac atgaaaatat gttttctgta acttccaact gtatttcaaa
    11821 aaaggagtgt cggacactcc ttaactatat taataggtga gatccatcat agctgttagt
    11881 tgattatgga taggttctcc actgctttcc ttgtaagctt gcaagtatta gtgacaaaga
    11941 aacaccagca atcactaaaa gaaccaacgc tgtcaaacta ataattaagt aaagcgtaga
    12001 acaatctagc cctttaacca taaggttgga attatagtct ttcgtatctc ttctatcgac
    12061 acattctctt tgaatccgaa aaataattcg aattttctac taatagacac acctctaaat
    12121 gacgttcata ttttttagtc gccaatatat aagtttccca atcaaactga taaactgggg
    12181 tgatttcgtt agcttggcca actaagttct gaattacgtc ttgaattaat ctaattaccg
    12241 ccttccccca cattctctga aaatttattt cagtataaca aaaaactaga acaccacaac
    12301 ggtgccctag cataataacc gcacagctct aaaacacata tcaggaatat aaaaatcgtt
    12361 caaatgtttt ggaaccattc gaaacagcac agctctaaaa ctgcctttta attcagctcc
    12421 cactttttat cgttttggaa ccattcgaaa cagcacagct ctaaaacttg tatatgaccc
    12481 gttaccgcta tcaaatagtt ttggaaccat tcgaaacagc acagctctaa aacttgtata
    12541 tgacccgtta ccgctatcaa atagttttgg aaccattcga aacagcacag ctctaaaacc
    12601 atgatgttat ggtatacgga caatacttag ttttggaacc attcgaaaca gcacagctct
    12661 aaaactaaat actttagaag aaagagttgg tttttgtttt ggaaccattc gaaacagcac
    12721 agctctaaaa ctcagaaagg ttgatagaat aatgtttcca agttttggaa ccattcgaaa
    12781 cagcacagct ctaaaacagt gtattcttgg ttatctttag ctatattgtt ttggaaccat
    12841 tcgaaacagc acagctctaa aacaccaaaa taagtcttta aaattgcttt ggcgttttgg
    12901 aaccattcga aacagcacag ctctaaaact agaaatcctt aggcgttcct cttaaagtag
    12961 ttttggaacc attcgaaaca gcacagctct aaaacttttc caatcacggt tcatgttcca
    13021 acggtgtttt ggaaccattc gaaacagcac agctctaaaa cttgactgat tttgcccaat
    13081 tgctcgtgca agttttggaa ccattcgaaa cagcacagct ctaaaacttt gcttctaaaa
    13141 aaaatacaga tgaagatgtt ttggaaccat tcgaaacagc acagctctaa aacctcgtag
    13201 aaaattttct tttgagcttt cgtaatcgcg ccattcgtct cagcaggact tcagtttcga
    13261 tgattccttg ttattactgt gcttttacta atattatacc atattttcgc ctatcaagaa
    13321 ataatcctta tcaataacat attgcggtaa atcatagagt cttctaggtt ctagaaagag
    13381 tactgacttt tgcattaaat tgatgtattc acataatttt ataacttcat ctttggtaag
    13441 ataagctccg ctattaacaa aaaccaagag attctttttc gttaaataat ggtaaacttg
    13501 tataatttca aaacattttt caaagatagt gtcgctctgt gtctcaattt tgactcctag
    13561 tgccttaatg agttctaaaa tcgtgatttc atcgtattct aaatcaagct cattctctag
    13621 acactcaaat gcgataagtt ctgtaatagt agctgctaat ttttctacca ttgatttcac
    13681 ttctggcttt tcgttgaatt gattttctaa gtcaccatgt atcaacttta gaataggtgc
    13741 tgagttgaca tcgtatccta agatatctgt aaccatcatt aactctgttg ccttcagact
    13801 tttctgctta tcatcaaata atttcaaatg ttcatctacg tcatattgat aaaaatgttt
    13861 caccaatgaa gaataaactg aaacatcttc tatcgttaaa atcgtagcat tacttaacac
    13921 taatggttca tctaaaattg gaaaattaat cttgatcata agaatctcct aggaaaacca
    13981 agcgactatc aaagttggca acacttgtgt tgcgttctcc atggaggtaa accattctcg
    14041 caaattgttt ttccgtaact gttaagagtg tgatattacc cttcttagga ttattcactt
    14101 taagccgacc tatcatagca ttattagctg tattattgag taataattta ctataaacag
    14161 agaactgatg cataataaaa ccttcgctca aaagaaactt tctaaactta cgatacgcct
    14221 tacgttcttc tactgtttca gtaggcatat caaacattaa aatcattctc atataccgat
    14281 aactcatatc ctaaactccg gaatcccatt cccatcacta tttagcgact taataacttt
    14341 tttggtgtaa tcgctgacaa tatttgaaag atacatttct ttaccgtcgt aactgtatgt
    14401 ctctgaaaac atagaaaaga gttctctttt cattttgaca aaatcactct gcctattttc
    14461 ataaataatc ctatcaacga ttgggcgaaa tggttccata atatcgcttg ctaggttgaa
    14521 ctgattaaat tgattagcat gctttaaccc aaattgtgtc atgcaaccac acttaacgac
    14581 ttcacgcgca aacatactca aaagcaatga atatccgtag tctaaaccag catttattgg
    14641 attatcatgt tctcttgaaa aatcatttcc aaaaagtgta ttgaaataaa tcctagctgc
    14701 atgcccttca cgattggaag tatcaaaaga ttctaaatca tgatagagat tcataataga
    14761 ctgggatttt tcaaagaaag aacactcacc aagataaaaa gactgattca aaattttttg
    14821 tgcaatgatt gacgtccaaa catccgcttt gacatcttca atccatgaca tctgcctaga
    14881 taattgtaaa ctcgaatcat atctggcata gtacggcatt aacatagctg ttggcaagcg
    14941 tttgtcatca caaaatataa ctaaaatatt ttcatcaacc aaacgtttaa tcagcatggt
    15001 cgacaaaacg atatctgttg tttccatgat tagaatgtca atctctgata gatgaatcat
    15061 ttccgtctga taagaatctt taaaaaccag atgattattt ttataagaga gcttagaatg
    15121 tgtatttaca acaacggttc gccaacctgc catatcaatc ttctcctaac ttacccaaat
    15181 caatacgtgt ttcataaagt ccagtaatag attgatgaat tagggtagaa ttaagtactt
    15241 ctttagttga tgtatagcgt tttctatcaa ctattttatc aaaaaattta aaagctgctg
    15301 gagctcctag actggtaaaa gtaaatagat tgataatatt attagcaagt tcatctactg
    15361 atatattttc cttattatct tggtaaagct tattgatttt ctctaaatta gcatctgcta
    15421 gaataactcg atttgaaaaa tcgttgatta tttgaagaat atcgtcgaaa taagaaacat
    15481 gttgaactac aaattcctgt ttttgttcaa tttcttctgg ttttcctttt agctcattgt
    15541 aacgacttgc aaggtataag aacttcatat attgtgttgg taaggctagc tcgttacctt
    15601 tttgtaattc accagcacta gcaagtaatc gacgacgccc attttctaat tcgaacagac
    15661 tatacttcgg taaaatcatt aatttatcgt ccctaatatt taaataacct tttgattcaa
    15721 ggaaagctga tggatttttc tcaaatctga atctctccat gatggtaatt cctaaaagtt
    15781 ccgtaactgt ttttagtttt tgtgctttac cttttttgat atcagctaca actaaaacag
    15841 agtaagctac tatcggacta tcaaaacctc catatttctt aggatctaaa taaatatcct
    15901 tcgtttttct tggaatcaac ttatctgagt taccatgcgc caagattgat tccttagaga
    15961 aaccacctgt ctgaatctct gtcttcttca cgatattgac ctgagggtat gacaagactt
    16021 ttctaactgt cgcaaagtgt ttcttcttat cccaaacaat ttcacccgta tcattattaa
    16081 cttcaatatc atcttttaca acaacggttc catccgccaa agttacctta gttttaaaga
    16141 agttcataat atttgaatag aaaaatagct tttctgtagc ggatttacgc gttttgtaac
    16201 tgttatattt tggatagtca ccgtagacaa attctggctc taactgagga tatttggtta
    16261 gaatagcttt agcaactact gcattaagat aggcatcatg tgcatggtga taatcattaa
    16321 cttcacgaat tttatagaat ccaaattctt ttcggaaatt tgaaaccaaa tttgacttca
    16381 aggttacaat tttaactttg cgaatccttc tacctttact atcaagctca ttattaaagc
    16441 gttcatccaa gatacgggca acatgcttgg taatttgtcg tgtctcaacc aactgacgtt
    16501 ggataaatct tgccttatca tcggaagtta ggcctccgcg ctctgcctta gtcaaattat
    16561 catacttacg ctgactcatt aacttagcat caagtaattt tttccagaat gctttgcgtg
    16621 cttttacaat atcaatgcta ggaacattat ctgattttcc acggttttta gcagatgata
    16681 ccaaaacacg attatcaata gaatcatctt ttatgaaagc ttgaggaaca atgtgatcaa
    16741 tatcatattg acttaaattg tcaatatcta gtgcttcccc tgtgtacata tctcttccat
    16801 tttgtaagta ataaaggaaa agtctttcat tttgcaacgc ttgattatcc gtaggatatt
    16861 ctttcaaaat attgccattc aagtcactag ctagattctt aacgccttcc tcaagaagtt
    16921 tatagcgttg tcgagtgtta cgacgacctt gatttgttgt ttgattctca cgcgccatct
    16981 caaccacaac ttgttcaggt tcgtatccca tgactttaac aagctcatca acaattttca
    17041 aactttgtag aatccccttt ttaatagcag ggctacctgc aagctcacct acaacttctt
    17101 ttagattatc tgaatgacta ccagcctgtg ccttgtcaat aattggttta aatgatagac
    17161 catcatcatg tatcaactgc ataaagttgc gatttgctct tccatcagaa atcagatagt
    17221 ccaagattgt tttttgactc tctttatctc taatgccatt aattaactta gcagacaatc
    17281 gtccccagcc agtatagtga cggcgataga gtttttttag ttgtgactct gtaaaaagat
    17341 ctttatagtt ttcaagacgc ttcttaatca tttctctatc ttcaaataag gttagagttt
    17401 ggacgatatc ttccaaaata gactcattat ctggattatc aagaaaatct ttgtctagta
    17461 tttttttgag atcgtggtaa gttcccaatg aggcgttgaa agctttattt tctttatcta
    17521 gaccaataac atctactatc ctaaactcct catattcttt agccagaaaa tctagcaact
    17581 tcttcttgga taccttacga tgttccttga atactccatc aaagatttct tgtttaatat
    17641 tgctatcaaa aaaataagtc tcaccttgct catttttata tctaacctta gtcaactcat
    17701 tatagaccgt aaatttttca taaataagac tatgttttgg taggactttt tcttcaggaa
    17761 gatagaggtc attgttggtc atacgatgga taaaagcttc cgcagatgct tccttatcaa
    17821 ccaattcttc aaaattccaa ggtcgaatac tgtcatctgt tttgcgagtc atccatgcaa
    17881 aatcactctt ctcacgtgct agtggcccga cataataagg aattctaaag gtaaggattt
    17941 tttcaatcct atctagattc tctttcaaga atggatagta ttctgattga cgtcgaataa
    18001 tagccttcaa ttctgtcaaa tggacttggt gtggaattga gccattatca aaggtccttt
    18061 gttttctcaa aaaatcttca ttcttgattt tttcaagaaa atactcgcta ccttcttgct
    18121 tggtcaacaa ttttgacaag tatttataaa atgctccttg attagttttg ccttcaatat
    18181 aaccagcgta gccatctttt gatgaatcag taaatatttc ttgatatttc tcaggcaatg
    18241 aagcttttac gaattgtttt aactgtttca agtcctctct atgttcatcg taacgctgaa
    18301 tcatagaggc agaaagtggc gccttggtac tgagatcagt tactgtaaga atgccagata
    18361 aaagaacact atcatatagc tttttcgcta ctgagaataa gtcagcaaat tcatcaccaa
    18421 tctgcccaag aagattttct aaatcttcat cgtagctatc cttagcaaat tgaagcggcg
    18481 ttttatcttc caaattgaaa tgtttcttga agtcagcttg atttccgaca atcaatttca
    18541 aaaattctgc aaaaatgcca gtagattttt ggttagggta ctgcgctaaa atgcgatctt
    18601 tcttcgcaga cttgctaatc ttatctgtta gaattccctc tacatctatg ttttgagata
    18661 acaaatggtt attttcgaag gtagtatcaa agatttctaa aaaagcttga tattgtcttt
    18721 gaatatcagt attcctcaca tcaaagctat catcctcaat taggaaatgt cctctgaatt
    18781 taatgatatg agctagagcc agataaacaa gacgaaggtc tgctttttct ttcttgtcag
    18841 ctaattcttt tctcaagtga taaatcgttg ggaacttttc atgataatct ttctcctcct
    18901 gcattgttgc aaagatagga tacttgctcc ctctcttgtc gtcctcaact agaaaagaat
    18961 cctctaatcg atgaaagaaa ctatcatcaa ctttactcat ttcctctgca aaaatttctt
    19021 gtaaatatag aatacgattt ctacgacgtg tataacgacg gcgagcagtt cgcttcaagc
    19081 gtctatctga agcagtattt ccaccatcaa aaagcagagc acctataaga ttcttcttta
    19141 tgtattcttt atctgtattc cctaagaccc tcatcttctt agcaggtacc ttataatcat
    19201 ctgtaaccac agaatatccg acggaattag ttccgatgtc gagacctatt gaatatggtt
    19261 tgttc
//
LOCUS       DATRQA010000027        18696 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.027, whole genome shotgun sequence.
ACCESSION   DATRQA010000027 DATRQA010000000
VERSION     DATRQA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18696
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.027"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            25..417
                     /locus_tag="VBD27_000685"
     CDS             25..417
                     /locus_tag="VBD27_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 domain-containing protein"
                     /protein_id="HEO7024716.1"
                     /translation="MKSIKLLSFIVIILLGLGFTVGGLKVTENNKQQELWEIANSKDA
                     KNAYQKWIYAEDEDAFKENAVIKSYDIDKESIKKNPMGGISVRLIINKDPNLYITCNL
                     DRDNQGHLVSQSSHQSPQLTHLLESRGH"
     gene            417..1709
                     /locus_tag="VBD27_000686"
     CDS             417..1709
                     /locus_tag="VBD27_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7024717.1"
                     /translation="MEKVLTDLDRVNIAKQEYELGSQLDTLVKIMSQDKVLPIGKVAH
                     VQDGGKETGEQIYTITPNGTLDKPEDVKEVTVLFKGSTAPFGGDDWKTDWFKNDIPIA
                     SKLLLKKFGSQSVSHKQGTKQLEQSAHLLKEVMNKYPNAKISVYGHSLGSMNAQYAIA
                     ELDDNQIYRVQGAWIYNGPNLYSILTDKQKEQVDKIKGVVNNYVDSKDKIGIGYPKEG
                     SDNAVGIVKIVDSKAVSGGSWVANFTNQHIWGGYQYNPDGSLKLAEDKFAYKENYSVV
                     HTNVNQRMHAIKAKLSSFKSTGKEVSKNQAIFLDSQQAKVISSGLVTISKEAVEEVKK
                     VEQKAVAKAEELYQSLKVAPTGVTELSPQELEEAYAQSGVSYASIVGEAQTFFSNKTQ
                     AAESISHAYVELENQINKGVEAMLRQDSNLSGEFKQWH"
     gene            1700..2092
                     /locus_tag="VBD27_000687"
     CDS             1700..2092
                     /locus_tag="VBD27_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024718.1"
                     /translation="MALIDIIEKQLADTQRKISDLDDAYHHSCCQFEEKLDDLSVRKN
                     KIINMLQETYDAVEYDLRYSNDSSDMMTLNRILGSYHDDLEQAYHKEYYALSAQEEEY
                     RANYIRQRSEHELTFEELQREKKRELMK"
     gene            2102..2383
                     /locus_tag="VBD27_000688"
     CDS             2102..2383
                     /locus_tag="VBD27_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024719.1"
                     /translation="MSLMWDVLTGKESVRTLKKQLDKELIHSVYAPRKRSKFHDIITS
                     HHKEIDEVAINTKTAIHKVANQLDEAIKGEFRSKVEEAIESNQQKYNHL"
     gene            2565..3347
                     /locus_tag="VBD27_000689"
     CDS             2565..3347
                     /locus_tag="VBD27_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO7024720.1"
                     /translation="MIKIFDTHTHLNVENFEGKIDEEINLASELGVTKMNVVGFDQDT
                     ISKSLELSSQYAQVYSTIGWHPTEAGSYDDNIESMIISHLENPKVIALGEIGLDYYWM
                     EDPKDIQIEVFKRQIELSKEYNLPFVVHTRDALEDTYEVIKESGVGPFGGIMHSFSGS
                     LEMAQKFIDLGMMISFSGVVTFKKALDVQEAARELPLDKILVETDAPYLAPVPKRGRE
                     NKTAYTRYVVEKIAELRGITVEEVAEATYQNAVRIFRLDEKN"
     gene            3334..3894
                     /gene="rnmV"
                     /locus_tag="VBD27_000690"
     CDS             3334..3894
                     /gene="rnmV"
                     /locus_tag="VBD27_000690"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEO7024721.1"
                     /translation="MKKIDIQEVIVVEGKDDTANLRRFYNVDTYETRGSAIDEDDLER
                     IERLHNLRGVIVFTDPDYNGERIRKIIMNAIPTVRHAFLNRDEAKPGSKTKGRSLGVE
                     HASFEDLQKALSKVTQHFDDEDHFDITQADLIRWGFITASDSRKRREYLGNQLRIGYS
                     NGKQLLKRLRLFGVTKAEVEECMEGY"
     gene            3913..4404
                     /locus_tag="VBD27_000691"
     CDS             3913..4404
                     /locus_tag="VBD27_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024722.1"
                     /translation="MEYNVTEALTRAGVDLATLAVKGTATMVTSKIQSIKDVKDAATL
                     RATYDDLINELLAEREQAIRIAQSYKEEYEKINIDDEDIEYLSNTLEQGISLLYNFSG
                     LNEEQERTMKQLVTLLNKDTLKTMQLIGFNYKQAIGEPLTEACATAIKNKFITQNSKG
                     KRK"
     gene            4484..5356
                     /gene="rsmA"
                     /locus_tag="VBD27_000692"
     CDS             4484..5356
                     /gene="rsmA"
                     /locus_tag="VBD27_000692"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO7024723.1"
                     /translation="MRIADKTVTRAILERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DKGVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLARFDNVQVVNQDIL
                     KADLQTQIQAFKNPDLPIKVVANLPYYITTPILMHLIESKIPFAEFVVMIQKEVADRI
                     SAMPNTKAYGSLSIAVQYYMTAKVSFIVPRTVFVPAPNVDSAILKMVCRDQPVVSVQD
                     EDFFFRVSKVAFVHRRKTLWNNLTSHFGKSEDTKAKLEKALEIAKIKPSIRGEALSIP
                     DFASLADALKEVGI"
     gene            5360..6211
                     /locus_tag="VBD27_000693"
     CDS             5360..6211
                     /locus_tag="VBD27_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO7024724.1"
                     /translation="MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSDNHCFDLSK
                     FGYVNLLGGKKVDEHYDKKSFENRQLVLENGYYNHILEAISKVLENNSQFHSVLDIGC
                     GEGFYSRQLVNKHEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQDSSIDI
                     ILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDHF
                     RENFEIISEQKVVQSYNCSQQERQAFIDMTPLLFSVDKTTIDWASISEITVGALIVIG
                     KKRSVSK"
     gene            6367..7239
                     /gene="rsgA"
                     /locus_tag="VBD27_000694"
     CDS             6367..7239
                     /gene="rsgA"
                     /locus_tag="VBD27_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEO7024725.1"
                     /translation="MQGRIVKSLAGFYYVESDGVVYQTRARGNFRKKGQIPYVGDWVE
                     FSSQDQSEGYILSIEERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEYK
                     MIQPIIYISKLDLLDDLVVIDDIRELYQNIGYVFCYSQEELLPLLANKVTVFMGQTGV
                     GKSTLLNKIAPELKLETGEISGSLGRGRHTTRAVSFYNVHKGKIADTPGFSSLDYEVD
                     NAEDLNESFPELRRLSHFCKFRSCTHTHEPKCAVKEALTQGQLWQVRYDNYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            7246..7908
                     /locus_tag="VBD27_000695"
     CDS             7246..7908
                     /locus_tag="VBD27_000695"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO7024726.1"
                     /translation="MSTNKIAPSILAADYANFANELKRIEETTAEYVHIDIMDGQFVP
                     NISFGADVVSSMRKHSKLVFDCHLMVVDPERYIEAFAQAGADIMTIHVEATKHIHGAL
                     QKIKEAGMKAGVVINPGTPVESLIPILDLVDQILIMTVNPGFGGQAFIPEMMSKVKTV
                     AAWRKEYGHHYDIEVDGGIDNTTIKAAAEAGANVFVAGSYLFKASDLPAQVETLRVAL
                     DD"
     gene            7901..8533
                     /locus_tag="VBD27_000696"
     CDS             7901..8533
                     /locus_tag="VBD27_000696"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO7024727.1"
                     /translation="MTKIALFAGGDLTYFEYDFDYFVGIDRGSLFLLKNGLSLDMAVG
                     DFDSITEDELLYIKHYCSNIVSASAEKNDTDTELALKTIFKEFPEAQVTVFGAFGGRI
                     DHMMSNIFLPSDRDLEPFMSQIRLKDEQNIVTYLPSGKNQVSRIEGMSYVSFMPESES
                     TLQISGAKYELNKSNYFKKKMYSSNEFMTSPIEVELKDGYLIIIYSKDRG"
     gene            8535..9809
                     /locus_tag="VBD27_000697"
     CDS             8535..9809
                     /locus_tag="VBD27_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEO7024728.1"
                     /translation="MLVIILIIVLASLTVTIISYPKMTELTKSVEKQLEDNADNLSDQ
                     LTYQIEVAQKDQILTLTNQLNRMQQEIYQLLTDMRTELNQHLTESRDRSDKRLELINS
                     NLSQSVQKMQDSNEKRLDQMRQTVEEKLEKTLQTRLQTSFETVSRQLESVNQGLGEMK
                     TVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTVSQYEREFPTVSGSSERVEYAI
                     KLPGNGQGDYIYLPIDSKFPLEDYYRLEDAYELGDKVQIELYRKSLLASIRKFAKDIN
                     NKYLNPPETTNFGIMFLPTEGLYSEVVRNATFFDSLRRDENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNANDISKILGNVKVEFGKFGGMLSKAQKQLNTASKSIDSLLTTRTNAI
                     IRVLNTVEEHQDQATTSLLNLPITEEEEINEN"
     gene            9799..10740
                     /locus_tag="VBD27_000698"
     CDS             9799..10740
                     /locus_tag="VBD27_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEO7024729.1"
                     /translation="MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFQDDTGEI
                     SGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGTPQVNQITLRNIKDGEPNDPRDFKEK
                     PPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHIS
                     LIDEELTKILAELNIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALNRVNEGEMTNRIFAMDNRSFYKPNIK"
     gene            10837..11649
                     /gene="purR"
                     /locus_tag="VBD27_000699"
     CDS             10837..11649
                     /gene="purR"
                     /locus_tag="VBD27_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEO7024730.1"
                     /translation="MKLRRSERMVVISNYLINNPYTLTSLNTFASKYGAAKSSISEDI
                     AIIKKAFEQAQIGDIKTVTGASGGVIFTPTIAEAEAKEIVEELRQRLSENDRILPGGY
                     IYLSDLLSTPKMLQSIGRIIANAYRGQKIDAVMTVATKGVPLANAVANVLDVPFVIVR
                     RDLKITEGSTVSVNYASGSSGRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTVSGMISL
                     LSEFDSTLVGVAVFAENAQEQREKMAYKSLLRVSEIDVKNNRVSVEAGNIFD"
     gene            11868..12281
                     /gene="rpsL"
                     /locus_tag="VBD27_000700"
     CDS             11868..12281
                     /gene="rpsL"
                     /locus_tag="VBD27_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO7024731.1"
                     /translation="MPTINQLVRKPRKSKVEKSDSPALNIGYNSHRKVHTKLSAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            12303..12773
                     /gene="rpsG"
                     /locus_tag="VBD27_000701"
     CDS             12303..12773
                     /gene="rpsG"
                     /locus_tag="VBD27_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO7024732.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFEAIKEATGTDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            12928..15006
                     /gene="fusA"
                     /locus_tag="VBD27_000702"
     CDS             12928..15006
                     /gene="fusA"
                     /locus_tag="VBD27_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO7024733.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQSLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYVDQANEYREKLVEAV
                     ADTDEDLMMKYLEGEEITNEELMAAIRKATINVEFYPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDEEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     SKVILESIEVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQVVRAFVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNSGE"
     gene            15210..16220
                     /gene="gap"
                     /locus_tag="VBD27_000703"
     CDS             15210..16220
                     /gene="gap"
                     /locus_tag="VBD27_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO7024734.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGQFVKVSAEREPANIDWATDGVEIVLEATGFFAS
                     KEKAEQHIHENGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALQDNFGVKQGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLEKDVTVEEVNAAMKAAANDSYGYTED
                     PIVSSDIVGISYGSLFDATQTKVQTVDGNQLVKVVSWYDNEMSYTSQLVRTLEYFAKI
                     AK"
     gene            16429..17298
                     /locus_tag="VBD27_000704"
     CDS             16429..17298
                     /locus_tag="VBD27_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEO7024735.1"
                     /translation="MKTTKVFSVLSLSVAVFMMTACQSNHSTKKADMSPDKVEQKTKF
                     KNISLSSNKLLAKENTMSVLWYQNSAEAKALYLQGYNVAKMKLDDWLQKPSEKPYSII
                     LDLDETVLDNSPYQAKNIKDGSSFTPESWDKWVQKKSAKAVAGAKEFLKYANEKGIKI
                     YYVSDRTDAQVDATKENLEKEGIPVQGKDHLLFLKKGMKSKESRRQAVQKDTNLIMLF
                     GDNLVDFADFSKSSSTDREQLLTKLQSEFGSKFIVFPNPMYGSWESAIYQGKHLDVQK
                     QLKERQKMLHSYD"
     gene            17433..18629
                     /locus_tag="VBD27_000705"
     CDS             17433..18629
                     /locus_tag="VBD27_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO7024736.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGAKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
BASE COUNT         6293 a         3050 c         3853 g         5500 t
ORIGIN      
        1 tgttatatga taaaaggagg agagatgaag tcaataaaat tacttagttt tattgtaata
       61 atccttttag ggcttggttt cactgtagga ggtttaaaag tgactgaaaa caataaacaa
      121 caagaattat gggaaatagc taatagcaaa gacgctaaaa atgcttatca aaagtggatt
      181 tatgcagaag atgaagatgc tttcaaagag aatgctgtta ttaagtcgta tgatattgac
      241 aaagaaagta tcaaaaaaaa tcctatggga gggatttcgg taaggttaat cattaataaa
      301 gatcctaact tatatattac ttgtaatttg gatagggata atcaagggca tttggtcagt
      361 caaagtagcc atcaatctcc ccagttaact catttattgg aaagtagagg tcattaatgg
      421 aaaaagtctt gactgattta gatagggtaa atatagcgaa gcaagagtat gaattaggta
      481 gccaacttga tacactagtc aaaataatga gccaagataa ggtactcccc atcggtaaag
      541 ttgctcacgt tcaagatgga ggcaaagaaa ccggggaaca aatttatacc atcactccaa
      601 atggaacttt agacaagcca gaggatgtta aggaagttac ggttttattt aaggggtcaa
      661 cagcaccgtt tggaggggat gattggaaaa cagactggtt taaaaatgat attccaatag
      721 ctagtaaact attattaaag aaatttggtt cacaaagtgt atctcataaa caaggtacaa
      781 agcagttaga acaatcagct catctgctaa aagaagttat gaataaatat cctaacgcaa
      841 aaatttcggt ctacggacac tcgttagggt caatgaatgc acagtatgct attgcagaat
      901 tggatgataa tcaaatttac cgagttcaag gagcttggat atataacggt ccaaaccttt
      961 atagtatttt gacagataaa caaaaagaac aggttgataa aataaaaggg gtggtcaata
     1021 attacgttga ttccaaggat aaaattggaa taggttatcc taaagaagga agcgataatg
     1081 ctgtcggaat tgttaaaata gttgacagta aagctgtttc aggaggtagc tgggttgcta
     1141 attttaccaa tcagcatatt tggggaggct accagtacaa tccagatggt tctttaaaat
     1201 tagcagaaga taaatttgct tacaaagaaa actattcagt agttcatacg aatgtgaatc
     1261 aaagaatgca tgctattaaa gcgaaactta gtagctttaa aagtacggga aaagaagtta
     1321 gtaagaatca agctattttc ctagatagtc aacaagcaaa agtgatctct agtggtttgg
     1381 taacgatatc aaaagaagca gttgaagaag ttaaaaaagt tgagcaaaaa gcagttgcta
     1441 aagcagaaga gctttatcaa tctttaaaag tagctcctac aggagttact gaactaagtc
     1501 ctcaagaatt ggaagaagcg tatgctcagt caggtgttag ctatgcgagc attgtcggag
     1561 aagctcaaac ctttttctca aataaaactc aagcggcaga aagcatttct cacgcttacg
     1621 ttgaattaga aaaccaaatt aataaaggag ttgaagctat gttgcgacaa gatagtaact
     1681 tgtcagggga gtttaaacaa tggcactgat tgatatcatt gaaaaacaat tagctgatac
     1741 tcagagaaaa atatcagatt tagatgatgc ctatcaccat agttgttgcc agtttgaaga
     1801 aaaattagac gatctctcag taagaaaaaa taagattatt aatatgcttc aagaaactta
     1861 cgacgctgtt gaatatgatt tgagatatag taatgatagc tcagatatga tgacgctcaa
     1921 tcgtatttta ggttcttatc atgacgactt agagcaagct taccataaag agtactatgc
     1981 cttgtccgct caagaagaag aatatagagc taactatatt cggcaacgtt cagaacatga
     2041 actaaccttt gaagagttgc aaagggaaaa gaaaagagaa ttaatgaagt aggagaaatt
     2101 attgagttta atgtgggatg tcctgactgg taaggagtca gtaaggactt taaaaaaaca
     2161 attggataaa gaattgatac atagcgtgta cgcgccacgc aagagatcta agtttcacga
     2221 tattattacg tcgcatcata aagaaataga cgaagtagcc atcaacacaa agacagctat
     2281 tcataaagtt gctaatcaat tggacgaggc aataaaagga gaatttcgtt caaaggttga
     2341 ggaggctatt gaaagcaatc agcaaaaata taatcattta tagtctagtt tctctaccat
     2401 tagctagaca cttgtagagg atttttacag caagtattca aaagtcagag caaaaatgtt
     2461 ttgacttttt tgatttactc tttctgatca gaatgcttca gtcttatgtt ataataataa
     2521 agattaatgc ttgtagctac gataagcaaa gaaagaacga tgatatgata aaaatatttg
     2581 atacccatac ccaccttaac gtagaaaatt ttgaaggaaa gatagacgag gaaattaatt
     2641 tagctagcga actcggtgtt acaaaaatga atgttgtcgg ctttgaccaa gataccattt
     2701 caaaatcttt ggaattatct tcccaatatg cacaggtata tagcacaata ggatggcatc
     2761 caactgaagc tggttcttat gatgataata ttgagtcaat gattatcagt catttagaaa
     2821 atcccaaggt tattgcactt ggtgaaattg gtctggatta ttattggatg gaggatccca
     2881 aagatattca gatcgaggtg tttaagcgtc aaattgaact ttcaaaagag tataaccttc
     2941 cttttgtcgt tcatacacgt gatgcgttag aagacactta tgaagtcatt aaggaatctg
     3001 gagttgggcc ttttggtggt ataatgcact ctttttctgg ttctttggaa atggctcaga
     3061 aatttattga tttagggatg atgatttcct tttctggtgt tgtgaccttt aagaaggcat
     3121 tagatgtaca agaagctgca cgagagttgc cattagataa gatattagtg gaaacagatg
     3181 ctccttattt ggctcctgta cctaaaagag gtcgagaaaa caaaaccgct tatacgagat
     3241 atgtcgttga aaaaatagca gagctacgag gcataactgt ggaagaggta gcggaggcta
     3301 cataccaaaa tgcagtaagg atatttagac ttgatgaaaa aaattgatat tcaagaagtg
     3361 atcgttgtcg aagggaaaga tgatactgct aatctgcgac gcttttacaa tgttgatacc
     3421 tatgaaacta gaggttcagc aattgatgaa gatgatttag agcgtatcga acgtttgcac
     3481 aatttgcggg gagttatcgt ctttacagac ccagattata atggcgagcg tatccgtaaa
     3541 atcatcatga atgccattcc gacagtccgc catgccttcc tcaatcgaga cgaggcaaag
     3601 ccaggctcaa agaccaaggg acgatctctg ggtgtcgagc atgccagctt tgaagatttg
     3661 cagaaggcac tctcaaaagt gactcagcat ttcgatgatg aagatcattt cgatatcacc
     3721 caagcagacc tcattcgttg gggattcatt actgctagcg atagccgaaa acgccgtgag
     3781 tatcttggga atcaactccg tatcggctac tcaaatggta aacaactcct caaacgcctc
     3841 agactcttcg gagtgaccaa ggcagaagtt gaagagtgta tggaggggta ttaatagaaa
     3901 aggagaaaaa tagtggagta taatgtaacg gaagcattga ctagagctgg ggtagattta
     3961 gcaacgcttg cagtcaaagg gactgcaact atggtaacca gtaaaattca atcaataaag
     4021 gatgtaaaag atgctgcaac attgagagct acttatgatg atttaattaa cgaattatta
     4081 gctgaacggg agcaagcaat tcgcattgca cagtcatata aagaagagta tgaaaaaatt
     4141 aatatagatg atgaggatat tgaatatcta tccaatactc tcgagcaagg aatctcattg
     4201 ttatataatt tttctggtct aaatgaggaa caggaacgaa caatgaaaca attggttaca
     4261 ttattgaata aagatacttt aaaaacaatg cagttgattg gatttaatta taagcaagca
     4321 ataggagaac cactaaccga agcttgtgca actgcaatta aaaataagtt tataactcag
     4381 aatagtaaag gcaaaaggaa gtagtggtat aatccagttg taatatcaca aatagcctca
     4441 aatgttattt caaaaatgat caatattaag aaatagagga attatgagaa tcgcagataa
     4501 aaccgttacc cgcgctattt tggagcgtca tgggtttaca tttaagaaat cattcggtca
     4561 gaactttttg accgatacta atattttaca gaagattgtt gacacggcag agattgacaa
     4621 gggtgtcaac gtgattgaga ttggtcctgg tatcggggct ttgacagagt ttttggctga
     4681 gaatgcagct gaggttatgg cttttgagat tgatgatcgt ctcattccta tccttgctga
     4741 tacgcttgcg cgttttgaca atgtccaagt ggtcaatcag gatatcctca aggctgattt
     4801 gcagacccag attcaggctt tcaagaatcc tgatctgcct atcaaggtgg tcgcaaacct
     4861 accttactac atcacaacac caatccttat gcacttgatt gagagcaaga ttccttttgc
     4921 ggagtttgtg gtcatgatac aaaaagaagt ggcagatcgt atttcagcta tgccaaatac
     4981 taaagcatac ggttctttgt caatcgcagt gcagtattat atgactgcca aagtatcatt
     5041 tatcgttccg agaactgttt ttgttcctgc tccaaatgta gattcagcta ttttgaaaat
     5101 ggtctgtaga gatcaaccag tagtgagtgt tcaagatgag gatttctttt tcagagtgtc
     5161 aaaagtagcc ttcgtacatc gtcgtaaaac actttggaat aatctaacga gtcattttgg
     5221 aaaatcagaa gatactaaag caaaattgga aaaagcatta gaaatagcaa aaattaagcc
     5281 atctattcgc ggagaagcct tatctatccc tgattttgca agtttagcag atgccttgaa
     5341 agaggttggg atataaacta tgttggttga caagaagtgg cgttttgaag atagtgcaag
     5401 ttatttcgct tgtcctaaat gtcaaaatcc cttgataaaa gaaagtaaca gcctcaaatg
     5461 ctctgataat cattgttttg atttatccaa atttggatat gttaatcttc ttggaggaaa
     5521 gaaggtagat gaacattatg ataaaaaatc atttgaaaat cggcagcttg tcttagaaaa
     5581 cgggtattat aatcatattt tagaagctat ttcaaaggtt ttggaaaata attctcagtt
     5641 tcattcggtt ttagatattg gttgtggaga gggcttttat tctcgtcaat tagttaacaa
     5701 gcatgagaag actttcttgg cttttgatat ttctaaagat tcgattcaat tagctgctaa
     5761 aagtgatcag tctaggcttg tgaaatggtt tgtttctgat ttagctaacc ttcctattca
     5821 agactcatca attgatatca ttttagacat tttttctcct gcaaattata aagaatttag
     5881 gcgggtgctg agtgatgatg gtatccttgt aaaggttgtt cctgtagctg aacatgttca
     5941 agaattgcga gaaaaagcta gtcaatactt gaaacagaaa gattactcta atcaaaaaat
     6001 tctagatcac tttagagaaa attttgaaat tatttctgag cagaaagttg ttcaaagcta
     6061 taactgttct caacaagaac gtcaagcttt tattgatatg acaccgttgt tattcagcgt
     6121 agataaaacg actattgatt gggcgagtat atcagaaatt acagtaggtg ccttaatagt
     6181 aattggaaaa aagcgcagtg tatctaaata agatcactgc ttttatgttg ggaattcata
     6241 aaattgaggt tatttgagag aaatcgaaca cctgcaaata gtttctattt agcttatctc
     6301 tgagagtaga gcttatttct cattttatga tataatagtg gtattctaaa caattaagga
     6361 gaacaattgc aaggtagaat tgtcaagtcg ctagcaggtt tttattatgt ggaatctgac
     6421 ggtgttgttt atcagacacg tgcgcgtgga aattttagaa aaaaaggtca aattccctac
     6481 gttggggatt gggttgaatt ttcttcgcaa gatcaatcag aaggatatat tctatcaatt
     6541 gaagagcgta aaaatagcct cgtacgacca cctattgtta atattgatca agctgtcgtt
     6601 atcatgtctg ctaaagaacc tgattttaac gctaacttat tagaccggtt cttagtactt
     6661 ctagaatata agatgattca gcctatcatt tatatttcca agttggattt gttagacgat
     6721 ttggttgtta ttgatgatat tcgcgaactt tatcagaata taggctacgt tttctgttat
     6781 tctcaagagg aactcttgcc tctgttagca aataaagtga ctgtttttat gggccagaca
     6841 ggtgtcggaa aatcaacgct tctcaacaaa attgctcctg agttgaaact tgaaactggt
     6901 gaaatttcgg gtagtttagg ccgtggccga cacaccacaa gagcagttag tttctataac
     6961 gttcataaag gaaaaattgc agacactcct ggtttttctt ctttggacta tgaagtggat
     7021 aatgcagagg acttaaacga atcctttcca gagcttcgta gacttagtca tttttgtaaa
     7081 ttccgctcgt gtacgcatac acatgagcct aaatgtgctg taaaagaagc cttaactcaa
     7141 ggtcagttat ggcaagtccg ctatgataac tacctccaat ttttaagtga aattgagaac
     7201 cgtcgtgaaa catataagaa agttattaaa agaaagtagg tttctatgtc aaccaataaa
     7261 attgccccat cgatattagc agcagactat gctaattttg ctaacgaatt gaaacgtatt
     7321 gaagagacaa cagcagaata cgttcatatt gatatcatgg atggtcaatt tgtccctaat
     7381 attagttttg gtgcggatgt tgtatccagc atgcgtaaac acagtaaact tgtttttgat
     7441 tgtcatctga tggttgttga tccagaacgt tatattgagg cttttgctca agctggtgct
     7501 gatatcatga ccattcatgt tgaagcaacg aaacatatac atggtgcgct tcaaaagata
     7561 aaagaggcag gaatgaaagc aggtgtggtt attaacccag gaacacccgt agaatctctt
     7621 attcctatct tagatttggt tgaccagatt ttaattatga ctgttaatcc tggctttggt
     7681 ggacaagcct ttattccaga aatgatgtca aaagtaaaga cagttgcagc atggcgcaaa
     7741 gagtacggtc atcattatga tattgaagtt gatggcggta tagataatac tacaattaag
     7801 gcagcagcag aggctggtgc aaatgtcttt gttgcaggtt cttatctctt taaagcaagt
     7861 gatttacctg cacaagtaga aaccttaagg gtggcgctcg atgactaaaa ttgctttatt
     7921 tgctggcggt gacttaactt attttgaata tgattttgac tattttgtcg gaattgaccg
     7981 tggaagccta tttttattaa aaaatgggct ttctttggac atggcagtgg gtgattttga
     8041 ttctataaca gaagatgaac tgctttatat caaacactat tgtagcaata tcgtttctgc
     8101 tagtgcagaa aaaaatgata cagatacaga attagcttta aaaactattt tcaaagaatt
     8161 tccagaagct caggtgaccg tatttggagc atttggtggc aggattgacc atatgatgtc
     8221 taatatcttt ttaccaagtg acagagatct agaaccattt atgtcacaaa tcagactcaa
     8281 agatgaacaa aacatagtga cttatctacc atccgggaaa aatcaagtct caaggattga
     8341 agggatgtcc tatgtctcgt tcatgcctga gagtgagtct actttgcaga tttctggggc
     8401 taaatatgaa ttgaacaaaa gtaattattt taagaaaaaa atgtattcca gtaatgaatt
     8461 tatgacttcg ccaattgaag tagagctcaa agatggttac ttaatcatta tttatagtaa
     8521 ggatagaggt taatatgctt gtcatcattt tgatcattgt actagctagt ctgacagtga
     8581 cgataatttc ttacccaaaa atgacggaat taacaaagtc cgttgaaaaa caacttgaag
     8641 ataatgctga taatctatca gaccaactga catatcagat agaagtggcg caaaaagatc
     8701 aaattctaac gttgactaat cagctaaacc gtatgcaaca ggaaatttat cagttgctta
     8761 ccgatatgcg tacagaatta aatcagcatt taacggagag tcgagatcga tcagacaaac
     8821 gcttggaatt gattaactcc aatttatctc agtcagttca gaaaatgcaa gattccaatg
     8881 aaaaacgctt ggatcaaatg cgccaaactg ttgaggaaaa gctagaaaaa acgctacaaa
     8941 cgcgtttgca aacttctttt gaaactgtat cgcgtcaact agagagcgtc aatcaaggtc
     9001 ttggggagat gaaaactgtt gcccaagatg ttggtacact gaacaaagtc ttgtcaaata
     9061 ctaagacaag gggaatatta ggggagttac aactcggcca aattatagaa gatattatga
     9121 cagttagtca atatgagaga gaatttccta cggtgtctgg ctcttctgag cgtgttgaat
     9181 atgctattaa attacctgga aatggtcagg gagattatat ctatttgcct attgactcta
     9241 agtttcctct agaagattat taccgattgg aagatgctta tgaattgggt gacaaggttc
     9301 aaatagaact ctatcgtaaa tctttactgg caagtattcg taaatttgca aaagatataa
     9361 acaataagta cttaaatcct cctgaaacga caaattttgg tatcatgttc ttaccaactg
     9421 aagggctcta ttctgaagtg gtaagaaatg caacattctt tgatagtcta agacgtgacg
     9481 aaaatattgt agtagctgga ccgtcaacct tatctgcttt actaaattca ttatctgttg
     9541 gctttaaaac cttaaatatt caaaagaatg ctaatgatat tagtaaaatt ttagggaatg
     9601 tcaaggttga atttggaaaa tttggtggca tgttatcaaa agctcaaaaa caattaaata
     9661 cagcaagtaa aagtattgat agtttactca caacgcggac aaacgctatt atcagagtct
     9721 taaatacagt tgaagaacat caagatcaag caaccactag cttgttaaac cttcctataa
     9781 cagaagaaga ggaaattaat gaaaattaac caaatgaaaa aagacgagct ttttgagggg
     9841 ttttacctta taaagaaagc agaagttcgt aaaacgcgtg caggtaaaga ctttattgcc
     9901 tttacctttc aagatgatac tggcgaaatt tcaggaaata tgtgggatgc acagacctat
     9961 aatgtagaag agtttgtagc cggtaaaatt gttcatatga aagggcgtcg tgaagtttat
    10021 aatggaactc cccaagtcaa ccaaattaca ttaagaaaca tcaaagatgg tgaacctaat
    10081 gatcctagag attttaaaga aaaacctcca atcaatgttg acaacgtgag agagtacatg
    10141 gaacaaatgc tctttaaaat tgaaaatgca acctggcagc gtgtggtaag agcactttat
    10201 cgtaaataca ataaggaatt ttttacctat ccagccgcca aaacaaacca ccacgctttt
    10261 gaatcaggat tggcatatca cacggcaaca atggttcgtt tggcagatag tatcggagat
    10321 atctatccag aacttaataa aagtttgatg tttgctggta ttatgctaca tgatttagcc
    10381 aaggtcattg agttatcggg tcctgataat acagaatata ctattcgagg taatcttatc
    10441 ggtcatattt cacttattga tgaggaatta acaaaaatac tagcagagct taatattgat
    10501 gatacaaaag aagaggttac agtgttacgc catgttattc tgagtcatca tggacaatta
    10561 gaatatggaa gcccggttcg tcctagaatc atggaggcag aaattatcca tatgatcgat
    10621 aacattgatg caaatatgat gatgatgaca acagctttaa accgtgtcaa cgaaggtgaa
    10681 atgacaaatc gcatttttgc catggataac cgttcttttt ataaaccgaa cattaaataa
    10741 tttaggtaga ataattaaga cctaaaataa aatgttcgtt tttagcattt gttatgttat
    10801 actatatgta aagaatgcta tgaataagga gaaaatatga aattaagacg tagcgaacgc
    10861 atggttgtta tttcaaatta cctaataaat aatccctata cattaactag tttaaataca
    10921 tttgctagta aatacggtgc cgcgaaatct tcgatttcag aagatattgc tattatcaaa
    10981 aaagcttttg aacaagctca aatcggagat attaaaactg tgactggtgc aagtggaggt
    11041 gttatcttta caccaactat tgctgaagca gaagcgaaag aaatcgttga agaattaaga
    11101 cagcgtttat ctgaaaacga tcgtattctt ccgggaggtt acatttatct ttcagatcta
    11161 ctcagtacac caaaaatgtt acaaagtatt ggtcgcatta tagcgaacgc ttacagaggt
    11221 caaaaaattg atgctgtcat gacggttgca acaaaaggtg ttcctttagc aaacgcagta
    11281 gcaaatgttt tagatgttcc ttttgttatt gtgagacgtg atttgaagat tacggaaggt
    11341 tctactgtgt cagtaaacta cgctagtggt tcaagtggcc gtattgaaaa aatgtttctt
    11401 tctaagcgta gcttaaaacc taacagtcga gttttaatag ttgatgattt cttgaaaggt
    11461 ggaggtactg tttcggggat gattagttta ctctcagaat tcgatagcac attagtaggt
    11521 gtcgctgtat ttgcagaaaa tgctcaggaa caacgtgaga agatggccta taaatctctt
    11581 ctgagagtat ctgaaattga tgtaaaaaat aatagagtct ctgtagaagc ggggaatata
    11641 tttgattaaa gagtcaaggt gacatccttg attctttttg cttgtaaaag taataaaagc
    11701 ttgacacatt atgtgtaaaa tgatactatg ataaacggta cttttacttt tggtctctca
    11761 aaagtgtaca gagacgtgct gacaattgtt gcaaaagtac acgcagatat aggctgtcac
    11821 caagtgctat atcaaccaaa aataaaaaac ttacaggaga aatgtagatg cctacaatta
    11881 accagttggt acgtaaacca cgtaaatcta aagttgaaaa atcagattca cctgctttga
    11941 atattggtta caacagtcac agaaaagttc acactaaatt atctgcacca caaaaacgtg
    12001 gtgttgcaac tcgtgttgga actatgacac ctaaaaaacc taactctgcc cttcgtaaat
    12061 ttgctcgtgt acgtttgagc aaccttattg aagttactgc ttacattcca ggtatcggac
    12121 acaacttgca agaacacagt gttgtgctta tccgtggtgg acgtgtaaaa gaccttccag
    12181 gggtacgtta ccatatcgtt cgtggtgcac ttgatactgc aggtgtagct gatcgtaaac
    12241 aaggccgttc taaatacggt gctaaacgtc ctaaagggta attagaaggg ggataagaga
    12301 aaatgagtcg taaaaatcaa gcgcctaaac gcgaagtatt gccagatcca ttgtataact
    12361 caaaaatcgt tacacgtctt atcaaccgtg taatgcttga tggtaaacgt ggtactgctg
    12421 caactatcgt atatgatgct tttgaagcaa ttaaagaagc tactggaaca gatgctcttg
    12481 aagtgttcga aacagctatg gaaaacatca tgcctgtact tgaggtacgt gcacgccgtg
    12541 ttggtggatc aaactaccaa gttcctgtcg aagttcgtcc agaacgtcgt acaactttag
    12601 gacttcgttg gttggttaac gcatcacgtg ctcgtggtga acacactatg aaagatcgtc
    12661 ttgcaaaaga aatcatggat gctgcaaaca acacaggagc atcagttaag aaacgtgaag
    12721 atactcacaa aatggctgaa gctaaccgtg cctttgcaca cttccgttgg taagattact
    12781 ctctttttga gacttatcat agctactaaa caaattgttt ggtagatagc aatgttagca
    12841 attagaacgg gtaggtcctg cctatccgtt ttttataaat agtgttataa taaagaagta
    12901 aataatttaa aatataggag aaaccaaatg gctcgcgaat tttcacttga aaaaactcgt
    12961 aatatcggta tcatggctca cgtcgatgct ggtaaaacaa caacaactga gcgtattctt
    13021 tactatactg gtaaaatcca caaaatcggt gaaacacacg aaggtgcttc acaaatggac
    13081 tggatggaac aagaacaaga acgtggtatc acaattacat cagctgcaac aacagcacaa
    13141 tgggatggtc accgtgttaa catcatcgat acaccaggac acgtggactt cacaattgaa
    13201 gttcaacgtt cacttcgcgt acttgatggt gcggtaacag ttcttgatgc tcaatcaggt
    13261 gtagaacctc aaactgaaac agtttggcgt caagcaacag aatacggagt tccacgtatc
    13321 gtttttgcta ataaaatgga taaaatcggt gctgacttcc tctattcagt acaatcactt
    13381 catgaccgtc ttcaagcgaa tgcacaccca attcaattgc caattggttc agaagacgat
    13441 ttccgtggta ttattgactt aatcaaaatg aaagctgaaa tctatactaa tgaccttggt
    13501 acagatatcc ttgaagaaga tattccagca gagtatgttg atcaagcaaa tgaatatcgt
    13561 gaaaaattag tagaagcagt tgctgatact gatgaagatt tgatgatgaa ataccttgaa
    13621 ggtgaagaaa tcactaacga agagttgatg gctgctattc gtaaagcaac tatcaatgtt
    13681 gaattctacc ctgtattgtg tggttcagcc ttcaagaata aaggtgttca attgatgctt
    13741 gatgctgtta ttgattacct tccaagccca cttgatattc ctgcaatcaa aggtattaac
    13801 cctgatactg acgaagaaga aacacgtcct gcttcagatg aagagccatt tgcagcgctt
    13861 gccttcaaga tcatgacaga tcctttcgtg ggtcgcttaa cattcttccg tgtgtactca
    13921 ggtgttctta attcaggttc atacgtgttg aatacatcta aaggtaaacg tgaacgtatc
    13981 ggacgtatct tacaaatgca cgcaaatagt cgtcaagaaa ttgaaactgt ttatgctggg
    14041 gatatcgcag cggctgttgg tttgaaagat acaactacag gtgattcatt gactgatgaa
    14101 aaatcaaaag ttattcttga atcaattgaa gttccagaac cagttatcca attgatggtt
    14161 gagccaaaat ctaaagctga ccaagataaa atgggtatcg cccttcaaaa acttgctgaa
    14221 gaagatccaa cgttccgcgt tgaaactaac gttgaaactg gtgaaacagt tatctcagga
    14281 atgggtgaac ttcaccttga tgtccttgtt gaccgtatga aacgtgaatt caaagttgaa
    14341 gcaaacgttg gtgcacctca agtatcatac cgcgaaacat tccgcgcttc tacacaagca
    14401 cgtggattct tcaaacgcca atctggtggt aaaggtcaat ttggtgatgt ttggattgaa
    14461 tttacaccaa acgaagaagg taaaggattc gaattcgaaa atgctatcgt cggtggtgtg
    14521 gttcctcgtg aatttattcc agccgttgaa aaaggacttg tagaatctat ggcaaacggt
    14581 gttcttgctg gatatccaat ggttgacgta aaagctaaac tttacgatgg ttcataccat
    14641 gatgttgact catctgagac agccttcaag attgctgcat cacttgcact taaagaagct
    14701 gctaaatcag cgcaaccagc tatccttgag ccaatgatgc ttgttacaat tacagctcca
    14761 gaagacaatc ttggtgacgt tatgggtcac gtgactgcac gtcgcggtcg tgttgatggt
    14821 atggaagcac gtggtaatac acaagttgtt cgtgcctttg ttccacttgc tgaaatgttt
    14881 ggttatgcaa cagttcttcg ttctgcaaca caaggacgtg gcacattcat gatggtattt
    14941 gaccactacg aagatgttcc taagtcagtt caagaagaaa tcatcaagaa aaactctggt
    15001 gaataatttc tgaaaactta tgaaaggtac tcgaaagagt atcttttttt ctttttgaaa
    15061 gtggtaaatt cattgcaatt tttctcgaaa aaagatataa taattgtgtt gaaagatttt
    15121 tgcacatatg tacaagttaa tcttttcaca ttgattaggg ggatccctaa aataaaatat
    15181 ttgagatttt cataaggagg aaatcactaa tggtagttaa agttggtatt aacggtttcg
    15241 gtcgtatcgg tcgtcttgca ttccgtcgca tccaaaacgt agaaggtgtt gaagttactc
    15301 gtatcaacga ccttacagat ccaaacatgc ttgcacactt gttgaaatat gacacaactc
    15361 aaggtcgttt cgacggtact gttgaagtta aagaaggtgg attcgaagtt aacggtcaat
    15421 ttgttaaagt ttctgctgaa cgcgaaccag caaacattga ctgggctact gatggcgtag
    15481 aaatcgttct tgaagcaact ggtttctttg catcaaaaga aaaagctgaa caacacatcc
    15541 atgaaaatgg tgctaaaaaa gttgttatca cagctcctgg tggaaacgac gttaaaacag
    15601 ttgttttcaa cactaaccac gatatccttg atggaactga aacagttatc tcaggtgctt
    15661 catgtactac aaactgtctt gctccaatgg ctaaagcttt acaagacaac tttggtgtta
    15721 aacaaggttt gatgactact atccacgcat acactggtga ccaaatgatc cttgacggac
    15781 cacaccgtgg tggtgacctt cgtcgtgctc gtgcaggtgc tgcaaacatc gttcctaact
    15841 caactggtgc tgcaaaagct atcggacttg ttatcccaga attgaacggt aaacttgatg
    15901 gtgctgcaca acgtgttcct gttccaactg gatcagtaac tgaattggtt gcaactcttg
    15961 aaaaagacgt aactgtcgaa gaagtaaatg cagctatgaa agcagcagct aacgattcat
    16021 acggttatac tgaagatcca atcgtatcat ctgatatcgt tggtatttca tacggttcat
    16081 tgtttgatgc tactcaaact aaagttcaaa ctgttgacgg taaccaattg gttaaagttg
    16141 tttcatggta cgataacgaa atgtcataca cttcacaact tgttcgtaca cttgaatact
    16201 ttgcaaaaat tgcaaaataa tttttaatta atatgatgta aaagagggat ttatccctct
    16261 tttattttta taaataaaaa ataatacgaa tgttttttca ttttattata gaaaattatc
    16321 agaaaataaa tgaaaaatga attatattga ctataagtta aaataaggtt tgattttgaa
    16381 agtaagcagt ggtatgattt aattgaaaaa atatagagga gaagttttat gaagactaca
    16441 aaagtttttt cagttttatc gttatctgtt gctgtattta tgatgacagc atgtcagtca
    16501 aaccacagta ctaaaaaagc tgatatgtct ccagataagg tggagcaaaa gacgaaattt
    16561 aaaaatattt ctctaagttc taataaactg ttggctaagg aaaacactat gtctgtcctt
    16621 tggtatcaaa attctgcaga agccaaggct ttatatttac aaggttataa tgttgctaaa
    16681 atgaagttag atgattggtt acaaaagccc agtgaaaaac catattcaat tatcttagat
    16741 ttagatgaaa cagttttaga taatagccca tatcaagcaa agaatattaa agatggctct
    16801 agtttcacgc cagagagttg ggataaatgg gtgcaaaaga aatcagctaa ggctgttgcg
    16861 ggtgccaaag aatttttgaa gtatgctaat gaaaagggaa taaaaattta ttatgtctca
    16921 gatcgtacag atgctcaagt tgatgcgact aaagaaaatt tagagaagga aggtatacct
    16981 gttcaaggga aagaccactt gcttttcctt aaaaaaggaa tgaaatctaa agagagtcgc
    17041 cgtcaggcag ttcaaaaaga taccaattta attatgcttt ttggagataa tttagttgat
    17101 tttgctgatt tttctaaatc atctagtaca gatagagaac aactactaac taaacttcaa
    17161 agtgagtttg gtagtaaatt tattgttttc ccaaatccta tgtacggttc ttgggaaagt
    17221 gctatttatc aaggaaaaca tctggatgtt caaaaacaat tgaaagaacg acaaaaaatg
    17281 ttgcattcgt atgattaata agtatacgaa ttctcttaaa aacaactagg aaaatctatg
    17341 aaatctattt gtgaaaaagt aatcaataat tacaactgtt cacaaaaata tgttataata
    17401 aataatatca aattatataa ggagttttac taatggctaa attgactgtt aaagacgttg
    17461 atttgaaagg taaaaaagtc ctcgttcgtg ttgactttaa tgtgcctttg aaagacggcg
    17521 ttatcactaa cgacaaccgt atcactgcgg ctcttccaac aatcaagtat atcatcgaac
    17581 aaggtggtcg tgctatcctc ttctctcacc ttggacgtgt taaagaagaa gctgacaaag
    17641 aaggaaaatc acttgcaccg gtagctgctg atttagctgc taaacttggt caagatgttg
    17701 tattcccagg tgttactcgt ggtgcaaaat tagaagaagc aatcaatgct ttggaagatg
    17761 gacaagttct tttggttgaa aacactcgtt ttgaagatgt tgacggtaag aaagaatcta
    17821 agaatgacga agaacttggt aaatactggg cttcacttgg agatggaatc ttcgttaacg
    17881 atgcatttgg tacagcacac cgtgctcatg catcaaacgt aggtatttca gcaaacgttg
    17941 aaaaagctgt agctggtttc cttcttgaaa acgaaattgc ttacatccaa gaagcagttg
    18001 aaactccaga acgcccattc gtagctattc ttggtggctc aaaagtttct gataagattg
    18061 gtgttatcga aaaccttctt gaaaaagctg ataaagttct tatcggtggt ggtatgactt
    18121 acacattcta caaagctcaa ggtatcgaaa tcggtaactc acttgtagaa gaagacaaat
    18181 tggatgttgc taaagacctc cttgaaaaat caaacggtaa attgatcttg ccagttgact
    18241 caaaagaagc aaacgcattt gctggttata ctgaagttcg cgacactgaa ggtgaagcag
    18301 tttcagaagg gttccttggt cttgacatcg gtcctaaatc aatcgctaaa tttgatgaag
    18361 cacttactgg tgctaaaaca gttgtatgga acggacctat gggtgtcttt gaaaaccctg
    18421 acttccaagc tggtacaatc ggtgtaatgg acgctatcgt taaacaacca ggcgttaaat
    18481 caatcatcgg tggtggtgat tcagcagcag ctgctatcaa ccttggccgt gctgacaaat
    18541 tctcatggat ctctactggt ggtggagcaa gcatggaatt gctcgaaggt aaagtattac
    18601 caggtttggc agcattgact gaaaaataat agtcattata atttatatta aaggacatgc
    18661 tgatgcatgt ctttttttag ttgataatgt agagtt
//
LOCUS       DATRQA010000028        18595 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.028, whole genome shotgun sequence.
ACCESSION   DATRQA010000028 DATRQA010000000
VERSION     DATRQA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18595
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.028"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            465..1827
                     /locus_tag="VBD27_000706"
                     /pseudo
     CDS             465..1827
                     /locus_tag="VBD27_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1999..2613)
                     /locus_tag="VBD27_000707"
     CDS             complement(1999..2613)
                     /locus_tag="VBD27_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO7024737.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSDEKIVEIIKEAVRQSPSAFNS
                     QTSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLF
                     FEDQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVID
                     ASVQAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
     gene            complement(2746..3171)
                     /locus_tag="VBD27_000708"
     CDS             complement(2746..3171)
                     /locus_tag="VBD27_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7024738.1"
                     /translation="MGNFKNSAVKSMVVMRKAFRTIDGKVSESFKEFELTPTQFAVLD
                     VLYAKGTMKIGELIENMLATSGNMTVVIKNMEKKGWVLRHSCPNDKRAFLVSLTTEGE
                     EVIKKALPEHIKRVEDAFSVLTETEQEDLINLLKKFKTL"
     gene            complement(3298..7704)
                     /locus_tag="VBD27_000709"
     CDS             complement(3298..7704)
                     /locus_tag="VBD27_000709"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEO7024739.1"
                     /translation="MSELFKKLMDQIEMPLEIKNSSVFSSADIIEVKVHSLSRLWEFH
                     FSFPELLPIEVYCELQTRLVNSFEKADIKATFDIRAETIDFSDDLLQDYYQQAFCEPL
                     CNSASFKSSFSQLKVHYNGSQMIISAPQFVNNNHFRQNHLPRLEQQFSLFGFGKLAID
                     MVSDEQMTQNLKSSFETNREQLLEKANQEAMQALEAQKSLEDSAPPSEEVTPTQNYDF
                     KERIKQRQAGFEKAEITPMIEVTTEENRIVFEGMVFSVERKTTRTGRHIINFKMTDYT
                     SSFAMQKWAKDDEELKKYDMISKGSWLRVRGNIENNNFTKSLTMNVQDIKEIVHHERK
                     DLMPADQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHPAIAITDHANVQSFPHGYH
                     RAKKAGIKAIFGLEANIVEDKVPISYNEVDMNLHEATYVVFDVETTGLSAANNDLIQI
                     AASKMFKGNIIEQFDEFIDPGHPLSDFTTELTGITDNHVRGSKPILQVLQEFQNFCQG
                     TVLVAHNATFDVGFMNANYERHNLPLITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQ
                     VALEHHHMANYDAEATGRLLFIFLKEARENRDVTNLMELNTKLVAEDSYKKARIKHAT
                     IYVQNQVGLKNIFKLVSLSNVKYFEGVARIPRSVLDAHREGLLLGTACSDGEVFDALL
                     SNGIDAAVTLAKYYDFIEVMPPAIYRPLVVRDLIKDEAGIQQIIRDLIEVGRRLDKPV
                     LATGNVHYIEPEDEIYREIIVRSLGQGAMINRTIGRGEDAQPAPLPKAHFRTTNEMLD
                     EFAFLGKDLAYEIVVTNTNTFADRFEDVEVVKGDLYTPFVDRAEERVAELTYAKAFEI
                     YGNPLPDIIDLRIEKELASILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVA
                     TMIGITEVNPMPPHYVCPNCQHSEFITDGSCGSGYDLPNKNCPKCGTLYKKDGQDIPF
                     ETFLGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEEYAFRAGTVGTVAEKTAFGFVK
                     GYERDYNKFYNDAEVERLATGAAGVKRSTGQHPGGIVVIPNYMDVYDFTPVQYPADDM
                     TAAWQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPSNILPDDPDVMKLFS
                     GTEVLGVTEEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWL
                     GNAQDLIKEGIATLSTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEDER
                     NGYIQAMRDNNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIFYYCAYF
                     SIRAKAFELRTMSAGLDAVKARMKDITEKRQRNEATNVENDLFTTLELVNEMLERGFK
                     FGKLDLYRSHATDFIIEEDTLIPPFVAMEGLGENVAKQIVRAREDGEFLSKTELRKRG
                     GVSSTLVEKFDEMGILGNLPEDNQLSLFDDFF"
     gene            complement(7827..8411)
                     /locus_tag="VBD27_000710"
     CDS             complement(7827..8411)
                     /locus_tag="VBD27_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO7024740.1"
                     /translation="MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASD
                     QTTTFIQTISPTAIEISKTYDLYASVLLAQAILESSSGQSDLSKAPNYNLFGIKGEYK
                     GKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASVWKSNTSSYK
                     DATAALTGLYATDTAYASKLNQIIETYSLDAYDK"
     gene            complement(8536..10389)
                     /locus_tag="VBD27_000711"
     CDS             complement(8536..10389)
                     /locus_tag="VBD27_000711"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO7024741.1"
                     /translation="MKQSKMLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMRQEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDQS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHKDYEDLDVTYEDYRKAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPNRT
                     DLNRWLVLDKTIPSIDDIPEDVLEEIKAELSAWLVSGEDTIAYSTESSYAANLEMATN
                     EYKPSTKAATFEEVTKVETPNCKSIDEVAGFLSIDENQTIKTLLFIADEQPVVALLVG
                     NDQVNDVKLKNYLAADFLEPASEEQAKEIFGAGFGSLGPVNLPESVKIIADRKVQDLA
                     NAVSGANQDGYHFTGVNPERDFTAEYVDIREVKEGEISPDGKGTLKFARGIEIGHIFK
                     LGTRYSDSMGANILDENGRSNPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGAYRF
                     AWGINFPEELAPFDVHLITVNVKDQESQDLTEKIEADLMLKGYEVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKASEGIVEVKIKASGDTIEVHADNLIETLEILTKK"
     gene            complement(10481..11740)
                     /gene="rseP"
                     /locus_tag="VBD27_000712"
     CDS             complement(10481..11740)
                     /gene="rseP"
                     /locus_tag="VBD27_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO7024742.1"
                     /translation="MLGILTFIIIFGVIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHIDKEGTTYTIRILPLGGYVRMAGWGDDKTEIKTGTPASLTLNKEGIVTRINLSGK
                     QLDNTSLPINVTAYDLEDKLTITGLVLSETKTYSVDHDATIIEEDGTEIRIAPLDVQY
                     QNASVWGRLITNFAGPMNNFILGLVVFIALAFIQGGVQDLSTNQVRVSENGPAASAGL
                     KNNDRILQIGSHKVSNWEQLTAAVEKSTSHLEKNQKLALKIKSKEVVKTINVKPQKVD
                     KSYIIGIMPALKTSFKDKLLGGFKLAWESFFRILNELKKLIAHFSINKLGGPVALYQA
                     SSQAAKNGFVTVLNLMGLISINLGIMNLIPIPALDGGKIVMNILEAIRRKPLKQETET
                     YITLAGVAVMLVLMIAVTWNDIMRAFF"
     gene            complement(11771..12565)
                     /locus_tag="VBD27_000713"
     CDS             complement(11771..12565)
                     /locus_tag="VBD27_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO7024743.1"
                     /translation="MKERVIWGAVALAIFIPFLVMGGLPFQFLVGLLAMIGVSELLRM
                     RRLEIFSFEGALAMIGAFVLTVPLDSYLSFLPVDASLSAYGIVIFMILAGTVLNSNSY
                     SFEDAAFPIASSFYVGIGFQNLVSARMAGIDKVLLALFIVWATDIGAYMIGRQFGQRK
                     LLPSVSPNKTIEGSLGGIASAIVVAFFFMLFDKTVYAPHSFLVMLVLVAIFSIFGQFG
                     DLVESSIKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHFFGLF"
     gene            complement(12580..13332)
                     /locus_tag="VBD27_000714"
     CDS             complement(12580..13332)
                     /locus_tag="VBD27_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEO7024744.1"
                     /translation="MFIFKRKKQVEMPLEKIPAHIGIIMDGNGRWAKKRLKPRVMGHK
                     AGMDALQEVTIAASELGVKVLTVYAFSTENWSRPDDEVKFIMNLPVEFFDKYVPELDK
                     NNVRVQVIGDTHKLPKATYDAMQRACLRTKHNSGLVLNFALNYGGRSEITNAIKEIAQ
                     DVLEAKLNPDDITEDLVANHLMTNSLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEF
                     YFTPVLWPDFKKDELHKAIVDYNQRHRRFGSV"
     gene            complement(13499..13840)
                     /gene="yajC"
                     /locus_tag="VBD27_000715"
     CDS             complement(13499..13840)
                     /gene="yajC"
                     /locus_tag="VBD27_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO7024745.1"
                     /translation="MSLPIIMLVVMVGMMFFMQRQQKKQAQERQKQLNAVQKGDEIVT
                     IGGLFGVVDEVNTEAQRMVLDVDGVYLTFELAAIKSVVSKAATPTEPVEDVKIIEVNE
                     DENKETAIETE"
     gene            complement(13917..14261)
                     /locus_tag="VBD27_000716"
     CDS             complement(13917..14261)
                     /locus_tag="VBD27_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO7024746.1"
                     /translation="MYSFEELLATMTLITAAEIEDKIDSNQDFVLFIGRISCPFCHLF
                     VPKIVEVADEDEFELFHLDSEDFDHWTANKDFRNKYDIPTVPGLMVVKNGTIKVKCDS
                     KMTKEEIREFIG"
     gene            complement(14452..15615)
                     /locus_tag="VBD27_000717"
     CDS             complement(14452..15615)
                     /locus_tag="VBD27_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEO7024747.1"
                     /translation="MSENLGQLAINQARENGGKLEVISKVKVEDKRDLSIAYTPGVAS
                     VSSAIAEDVELAYELTTKKNTVAVVSDGSAVLGLGDICPEAAMPVMEGKAALFKRFAN
                     VDAVPIVLKTNDTEEIISIVKAISPTFGGINLEDISAPRCFEIEQRLIEECDIPVFHD
                     DQHGTAIVVLAALFNSLKLVKKDIEDIKVVVNGGGSAGLSITRKLLSAGAKHVTVVDR
                     FGIINDKDRESLAPHHKAIAKLTNREFQSGSLEDALENADVFIGVSAPEALHAEWISK
                     MADKPIVFAMANPIPEIYPDQALKAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAARGIASLIPEEELSTTHIIPNAFQNDVADVVAKSVSNAVQK"
     gene            complement(15640..16977)
                     /locus_tag="VBD27_000718"
     CDS             complement(15640..16977)
                     /locus_tag="VBD27_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEO7024748.1"
                     /translation="MADVKVVNNEGSRGQKQDLKTKLFHIKVGSVPLPVYVCLALLIL
                     LAGFLQKLPVNMLGGFAVILTMGWFLGTIGASIPGFKNFGGPAILSLLVPSILVFFNL
                     INKNVLESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLLGMVCAM
                     MVGTFVGVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIP
                     ATIIGNFFAILCTALLNRLGEKKPHLSGQEQLVRLNKGEDMSDIIADHSGPIDVKKMG
                     GGVLTACSLFIFGHLLQQLTGFPGPVLMIIAAAILKYINVIPRETQNGAKQLYKFISG
                     NFTFPLMAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLNMNPVEAG
                     IISACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF"
     gene            17131..>18595
                     /locus_tag="VBD27_000719"
     CDS             17131..>18595
                     /locus_tag="VBD27_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7024749.1"
                     /translation="MKKKLSLWAFLSLILVTMTICIFSIFYYVTIHQSYRMVRVQEEK
                     ILKNTGYALSRNPQVIQTLKDNHYNQSLQKQMLFLSKKSNLDYIVLINLKGIRFTHPD
                     STKIGKPFQGGDEQAVFKGKAIMSTAEGSLGKSLRYLIPVYDHQKQVGAIAVGLKLTT
                     LGDLSQSSIKEFSKPLLISILISLVVTSIISYGLKKQLHNLHPSDIFQHLEERNATLD
                     QIQAAVFVIDQRHIIKRNNPAASLLFKKEGQRDLFSGKLLESLIPQLKQDHFSKKTEQ
                     VLHFQGQDYLLSISPITVKTQNRGYVVFLRNVTETLFTLDQLAHTTAYASALQAQTHQ
                     FMNQLHVIYGLADIEYYDELKIYLKELLEPQNEFLARLSMLVREPRLASFIIGEREKF
                     AEKHINLSTEILVEIPTKSTVEDVNNYLLLHRYINTKILTLLNSTTLVSLRLNYQNNL
                     IETDYQWENEKWLLNDYHQYFNDAYFQQLLVDSRATYK"
BASE COUNT         6221 a         3684 c         2910 g         5780 t
ORIGIN      
        1 gtgttttatc atttgctgta gcttcaacat tattcaacct atctgattta aatatatcat
       61 atgctaatca aaataatatt caagaaacta atctagttga aaaaaacagt gaagataagt
      121 ttattcaaga gttaaatagg tataaaacag agataccaaa tttcaaaggt tttaatgttt
      181 ggattttagg tgataaaggt tattacaaaa accttatcaa tttagaagaa attaaaaata
      241 tccaagctac attaaaaaaa gaaagaaatg aagaatatgt ctttgtcaaa ttaaatggga
      301 aaatcgcaca tgatacaaca gtattcttga tgaataaaaa acataaatta cttaaaaata
      361 tagaggaatt taagacgata acccaaaaaa ggctaactga gagaggaaag ttcccatatg
      421 ataccgttca ttctacgttt gaaattaagg atgaaaactt tatcatggaa agattgaaat
      481 catcagggtt atctatgggt aaaccagttg attatatggg cgtaaatggt atccctattt
      541 acactaaaac attatctata gataataagt ttgcctttga aaataattca aaagattctt
      601 catattcttc aaacattaat attagtgaag ataaaataaa agaaaatgac caaaaaattc
      661 tagatttaat agttaaatct ggagctaata atcaaaactt aacagatgaa gaaaaagtaa
      721 ttgctttcac taaatatata ggtgaaataa ctaattatga caatgaagct tacagagcta
      781 gaaatgtcga cacagaatat tacagagctt cagatttatt ttctgtaaca gaaagaaaac
      841 ttgctatgtg cgttggctat agtgtaacag ccgctagagc atttaatatt atggggatac
      901 ctagttatgt cgtttctggt aaaagtcctc aaggaatatc tcatgcagct gtgagagcgt
      961 attataatag aagttggcat atcatagata tcacagccag tacatattgg aaaaatggta
     1021 attacaaaac aacgtattcc gattttatta aagaatactg tatagatggt tatgacgttt
     1081 atgatcccgc aaaaacaaat aatagattta aagttaagta tatggaatca aatgaagcgt
     1141 ttgaaaattg gatacataat aatggaagca aatctatgtt atttattaat gaatctgcag
     1201 ctctaaaaga taaaaaacca aaagatgatt ttgtaccagt aacagaaaaa gaaaaaaacg
     1261 agctgataga taagtataaa aaattattgt cacagattcc tgaaaacaca caaaatccag
     1321 gagaaaaaaa cataagagat tatttgaaaa atgaatatga agaaatatta aagaaagata
     1381 atttatttga gcatgaacat gcagaattta aagaaagtct aaatttaaat gaatcattct
     1441 acctacaatt aaagaaagaa gaaaagaaac cgagtgataa tctcaaaaaa gaagaaaaac
     1501 caagagaaaa ttcagtaaaa gagagagaga ctccagctga aaacaatgat tttgtttcag
     1561 taacagaaaa aaacaacttg attgataaat ataaagaatt attatcaaaa atcccagaga
     1621 atacacaaaa tcctggggga aaagaatata cgaaattatc tagagaaaga atatgaagaa
     1681 ctactacaaa aagatagatt attcaagcat gagtacacag agtttacaaa aagtctaaat
     1741 ttgaatgaaa cattctattc acaattaaaa gaaggagaaa tgaaactaag tgaaaatccc
     1801 gaaaaaggag aaacgaacac gaattaacct ttcaaatatc tacatcaaat aacagaagtc
     1861 tccgtatcaa gcgaattgta gcttttttga atatactaaa aacactccta cctctatttt
     1921 actagaagta ggagtgtttt tttcatttta gttcatagat gcagtgccat tgctatcctt
     1981 gccttttttt gtacagtatt atccgatgac tttaaagcgg tcatcatcat tcatgaattc
     2041 tttttctcct gtttcagctt cgattgaacc aaaatttagt tgtccacgaa gtttccaact
     2101 agctggaaca ccataaacag cttgtactga tgcatcaata actggattgt aatgttgtaa
     2161 attaccacca agtccaagtt ctgcagaaag ggctgtccaa gtatttactg aggcaatacc
     2221 tgttgattgc tcagaccaaa ctggaaagtt atccgcataa agaacgaatt gttcttgaag
     2281 agatttgaca acatcttgat cttcaaagaa gagtactgtt ccctttgaag ctccaaaact
     2341 agctaatttt tccttagttc cagcaatagc tgtctctgga gcaccttgta ctttcattgt
     2401 ttctaccaaa tcattagcaa ccaattcatc ccagaactta gtaacttcat cattcaataa
     2461 aatgacaaca cgggaagttt gactattaaa agcagatggg gattggcgaa ctgcctcttt
     2521 aataatctct actattttct catcagaaac ttctgtattg cgaccaagag cgtaaataga
     2581 gcgacggttt tttaattctt ccaaaaatga catatttttt ctccttgttc ttcaatctca
     2641 gatatcttac tacatttgtt ctagtgacat atgaattaga ttaaagttat ttattactat
     2701 gtagtaatta tattaccact atatcgtaaa gtcaaagaat gtgactcaca aggttttaaa
     2761 tttttttaaa aggttaatca agtcttcttg ctctgtttct gttaagactg aaaaagcatc
     2821 ttctacacgt ttaatatgct ctggtagagc tttttttatc acttcttctc cttcagttgt
     2881 taaactcacc aaaaaggctc tcttatcatt cggacaagaa tggcgtaata cccatccctt
     2941 cttttccata tttttaataa caacagtcat attacctgat gtagcaagca tattttcaat
     3001 caattctcca attttcattg tgcctttagc atagagaaca tccaaaactg caaactgagt
     3061 tggtgttaat tcaaattctt taaatgattc tgagactttt ccgtctattg ttctaaatgc
     3121 ttttcgcatg acaaccatag atttaaccgc tgaattttta aagtttccca tttcatctcc
     3181 caaacgtatt ttttcttatt ttatcactaa ttagttattt taagcaagaa tatcatcttt
     3241 gccctatcaa aagcagctgg caaataataa ggttgagtaa ctactcaacc ttattgctta
     3301 gaagaaatca tcaaaaagac tcaactgatt gtcttctggt aaatttccca aaatacccat
     3361 ctcatcaaat ttttcaacta aggtacttga gactcctcct cgtttacgaa gctctgtttt
     3421 tgataaaaat tcaccatctt cacgtgctcg aactatctgt tttgcaacgt tttctcccaa
     3481 gccttccata gcaacaaatg gagggattag cgtatcctct tcaataataa agtcagtcgc
     3541 atggctacga taaagatcta atttcccaaa tttaaagcca cgctcaagca tttcattaac
     3601 caactctaat gtcgtaaaaa gatcattttc gacattcgtc gcctcattac gctgacgctt
     3661 ctcagtgata tccttcatgc gcgccttaac ggcatcaagt ccagcactca ttgtgcgtaa
     3721 ttcaaaagct ttggcgcgaa tagaaaaata agcacaataa taaaatattg gataatgtac
     3781 tttaaagtac gccacacgta aggccattaa aacataggct gctgcatggg ctttagggaa
     3841 catatacttg atttttccac acgattcaat ataccaatcc gggacattat tatcacgcat
     3901 tgcttggata taaccgttac gctcatcttc tgaaattttc aaccataacc ccttacgcac
     3961 gcgctccatg attgtaaagg ccatctttgg ttgtagacca gcgtgcatta aataaaccat
     4021 gatgtcatca cgacaaccaa taacggttga taaggtagca ataccttctt tgattaagtc
     4081 ttgagcatta cctaaccaaa catccgtacc atgtgatagt cctgacagtt gcaacaattc
     4141 tgcaaatgtg gttggatggg tttcgttcac cataccacga acaaagttcg taccaaactc
     4201 aggaatacca agcatccctg taggagttcc aatttgctct tctgtcactc ctaaaacttc
     4261 cgtaccagaa aataacttca taacgtctgg atcatctgga aggatatttg atggatcaat
     4321 tcctgataaa tcctgaagtt tacgaatcat agtaggatca tcatgtccta gaatatctaa
     4381 cttcaagaca ttctcatcaa tatcatggaa attgaagtga gttgtctgcc aagcagctgt
     4441 catatcatct gctggatatt gaaccggagt aaaatcataa acatccatat agttaggaat
     4501 aacaacgata ccaccggggt gttgacctgt agaacgtttg actcccgcag caccagtcgc
     4561 taaccgttcg acctctgcat cgttataaaa tttattgtaa tctcgttcgt aacctttgac
     4621 aaaaccaaag gcagtttttt cagctacggt acctaccgtt cctgcacgaa aagcatattc
     4681 ttctccaaag atatcacgaa catctaagtg agcagaaggt tgatcatctc cagagaaatt
     4741 tagatcaata tcaggaacct tatctccatc aaatcctaag aaagtttcaa acggaatatc
     4801 ctgtccatct ttcttgtaca aagtaccaca tttaggacaa tttttatttg gtaaatcgta
     4861 acctgaacca caagaaccat ctgtgataaa ttcagaatgc tgacaatttg ggcaaacata
     4921 atgaggtggc attggattaa cttcagtaat tcctatcatg gtcgctacaa aactcgaccc
     4981 aacagaccca cgtgatccta ctagataacc acgttcatta gaacgttgca ccaacatttg
     5041 cgaagcaaga tagataacgg caaatccatt accaagtata gatgctaact ctttttcgat
     5101 acgtaaatca ataatatcag gaagaggatt accatatatt tcgaatgctt tagcataagt
     5161 taattcagca actcgttctt cagcacgatc aacaaacggg gtatagagat ccccttttac
     5221 aacttcaacg tcttcaaatc gatctgcaaa agtattcgta ttggttacaa cgatctcata
     5281 ggctaaatct ttaccaagaa aggcaaattc atctaacatc tcatttgttg tacggaaatg
     5341 tgcttttggc aaaggcgctg gttgagcatc ctccccacga ccgatagtcc tattaatcat
     5401 ggcaccttgt ccaagactac gaacaataat ctcacggtaa atctcatctt caggctcaat
     5461 ataatgaaca tttcccgtag ctaaaacagg cttatctaaa cgccgaccga cctctattag
     5521 gtctctaata atttgttgaa tccccgcctc atctttaatt aaatcacgaa ccaccaaggg
     5581 gcgataaata gcaggtggca tgacttcaat aaaatcataa tatttagcta gggtcactgc
     5641 tgcatcaatt ccatttgata acaaggcatc aaaaacttct ccatcagaac aggcagtacc
     5701 tagcaagaga ccttctctgt gagcatctaa aactgatcta ggaattcttg ccaccccttc
     5761 aaaatattta acatttgaaa ggctaactaa cttaaatata tttttaagtc caacctgatt
     5821 ttggacataa atggttgcat gctttatgcg tgcctttttg taagagtctt cagctaccaa
     5881 tttagtattg agctccatca aattagtcac atcacgattt tcccgcgcct cttttaagaa
     5941 aataaagagt aagcgacccg ttgcttcagc atcgtagttg gccatatgat gatgttccaa
     6001 agcaacttgg aaacgtttgg ttagaggccc caaaccatga cgcttatatt caggataaag
     6061 atttcttgca aattctaagg tatcaatgac cggctgtgtg atcaaaggca gattatgacg
     6121 ttcataattc gcattcataa aaccaacgtc aaaggttgca ttatgcgcaa ctaaaaccgt
     6181 accttgacaa aaattctgaa attcctgaag gacttgtaaa ataggttttg aacctctgac
     6241 atgattatcc gtaatacctg tcaactcagt tgtaaagtca gaaagaggat ggcctgggtc
     6301 aataaactca tcaaattgct caataatatt acccttgaac atttttgatg ctgcaatctg
     6361 aattaggtca ttattagctg cagaaagacc tgtagtttcc acatcaaata caacataagt
     6421 tgcttcgtgc aaattcatat caacttcgtt gtaggaaata ggcactttat cttcaacaat
     6481 atttgcctct aatccaaaaa ttgcttttat acctgctttt tttgctctgt ggtagccgtg
     6541 cggaaaactt tgaacattgg catggtcagt aattgcaata gctgggtgtc cccacttagc
     6601 tgcagtatca atgaggctct cgacagttgg taaagcatcc atcgttgaca tattggtatg
     6661 agcatgaaat tccacacgtt tttgatctgc tggcattaag tctttacgtt catgatgcac
     6721 aatctctttg atatcttgaa cattcattgt caaagattta gtaaagttat tgttctcaat
     6781 attccctctg acccgcaacc acgaaccttt agaaatcata tcgtacttct tcaattcttc
     6841 atcatcttta gcccactttt gcatagcaaa acttgaggta taatcagtca tcttaaaatt
     6901 aatgatatga cgtccagtgc gggttgtttt tcgttcaaca ctaaaaacca ttccttcaaa
     6961 aacgatacga ttttcttctg tggtcacctc aatcatgggt gtaatctctg ctttttcaaa
     7021 gccagcttga cgttgtttga tacgctcttt gaaatcataa ttttgagttg gtgtcacttc
     7081 ttctgatgga ggcgctgaat cttctagtga tttttgtgct tccaaggctt gcatagcctc
     7141 ttggttagct ttttcaagca attgctcgcg gttagtttca aatgatgatt ttaagttttg
     7201 cgtcatttgt tcatcagaaa ccatatcaat agcaagttta ccaaatccaa acaatgaaaa
     7261 ttgttgttcc aagcgcggca aatgattctg acgaaaatga ttattattga caaattgagg
     7321 tgctgaaata atcatctgac tgccattgta atgaactttt aattgtgaaa aagaactctt
     7381 aaaacttgca ctattacaca agggttcaca aaaagcttgt tgataataat cctgtagcag
     7441 atcatcagaa aaatcaatag tttctgctct tatatcaaaa gtagccttta tatctgcttt
     7501 ttcaaaacta ttcactaaac gagtttgtaa ttcacaatac acctcaattg gcaataattc
     7561 tggaaatgaa aaatgaaatt cccataagcg tgatagagaa tgaaccttta cctcgataat
     7621 atcagcagac gaaaaaacac ttgaattctt aatttcaagc ggcatttcaa tctgatccat
     7681 aagtttcttg aacaattctg acatagtttt ctcctgaata tcttacttcc attttatcac
     7741 atatctgtaa atatttcgag ataacaaaag gaggctgtta aaaaaggcat ttattagcct
     7801 tcttaacaac actctcttaa ctgagactat ttatcataag catctagact gtaggtttca
     7861 ataatttggt ttaatttact agcataagca gtatctgtcg cataaagacc tgttagagct
     7921 gcagtagcat ccttataaga agaagtattt gatttccaaa cagatgcata cttttgacta
     7981 gatactaact cagcataatc atatagtgaa gcagaataat ttggataggc gcgaaaagga
     8041 gcttggattt gagtcatatt gcctttccca tcatcttcta aagtaggcat ttggacagat
     8101 ttacctttat attctccttt gatgccaaag aggttataat taggagcctt agacaaatct
     8161 gattgtccac tggatgattc caaaatagct tgtgctaata agactgacgc atacaaatca
     8221 taggtcttag aaatttcaat agctgttgga gaaatcgttt gaataaaagt cgtcgtttga
     8281 tccgatgcac tagtgactgt cggttcgaca ttatcatttt tataattgta aaaccaaacc
     8341 ccacccaaac caaaaactaa tagtgttgtt gttaacctca ataccaattt atcttttttt
     8401 cgacttttca tgatggctcc tttctaaaat atcatttcat tataacaaaa ggcagttgct
     8461 ttttaccaat aagaaccata ctaaatttca ctccatataa aaaagttagc acatcagact
     8521 aactttttta tcggcttatt tttttgttag aatctctaaa gtttcaatta agttatcagc
     8581 gtgaacctca atcgtatcac cacttgcttt aattttcact tcaacgatac cttcagaagc
     8641 tttcttccca acagttactc gaattggaag tccaattaag tcactgtctg agaatttaga
     8701 accaacacgt tcattacgat cgtctgtcag gacttcataa cctttaagca tcaaatcagc
     8761 ttcaattttc tcagtaagat cttgtgactc ttgatctttg acattgacag taatcaaatg
     8821 tacatcaaat ggggctaatt cttctgggaa attaatcccc caagcaaagc gataagctcc
     8881 ttttggtgtc ttattcacaa aaagtcgggc atgttgttca atgacagctg agagaatacg
     8941 actaacacca ataccataac accccataac aataggattt gagcggccat tttcatcaag
     9001 gatgtttgct cccatactat ctgaataacg ggtacctagt ttaaagatat gtccaatttc
     9061 aataccacga gcaaatttca gagtaccttt accgtctgga gaaatctccc cttcttttac
     9121 ttcacgaata tcaacgtatt cagctgtgaa atcacgttct ggattaactc cagtaaaatg
     9181 ataaccatct tggttagcac cagacacagc attagcaagg tcttgaactt tacgatcagc
     9241 aataatttta acagactcag gtaggttaac tggcccaaga gaaccaaaac ctgcaccaaa
     9301 aatctctttt gcttgctctt cacttgcagg ctctaagaaa tcagccgcta aataattttt
     9361 taacttcaca tcattgactt gatcgttacc aactagtaaa gcaaccacgg gctgttcatc
     9421 agcaataaac aatagtgtct tgatagtttg attttcatca attgataaaa atccagctac
     9481 ctcatcaatt gatttgcagt taggagtttc tacttttgtt acttcttcaa aggtagcagc
     9541 cttagtagat ggtttgtact cattcgttgc catctcaagg ttagcagcat agcttgattc
     9601 agtggagtat gcaatagtgt cctcaccaga aactagccaa gccgagagtt ctgccttaat
     9661 ttcttctaaa acatcctctg gaatatcatc aatagaagga atagttttat caaggactaa
     9721 ccaacgattt aaatctgtac gattgggagt tactgccata aattcttggc tatctttacc
     9781 tcccatagca cctccgtcac ctataatgcc tttaaagtca agtccagcac gtgtgaagat
     9841 ggcttcatat gctttacggt aatcttcgta agtgacatca aggtcttcat aatctttgtg
     9901 gaagctgtat ccatctttca tgataaattc acgtgttcga agaagtccat tacgaggacg
     9961 cttctcatca cgatatttag actgaatttg ataaaggttt agtggtaatt gcttataaga
    10021 ttttactgca tcacgaacaa gagttgtgaa agtctcttca tgagttggtc ctaagataaa
    10081 atccgattga tcacgattct ttaatttata aaggtcttca ccatatgttt cgtagcgccc
    10141 agattcacgc cataaatctg ctgtaagaag agctggagct aacatttcaa ctgcaccaat
    10201 cttctcaaat tcttgacgca taattgtttt aaatttttca atagtacggt tagctagagg
    10261 caaataagca tagattccag ccgaaacttg gcggacatat cctgcacgaa ccataagagc
    10321 atggctgata acctgagcat cacttggcat ttcgcgaagt gttggaataa gcattttact
    10381 ttgtttcatt acaataagat acgactaatc aaagttgatt ctttttcaat tgagaattgc
    10441 aattgaaaaa ttcgtatttc ctttcacgtt ttattatctt ttagaagaaa gctcgcatga
    10501 tatcgttcca ggttacagca atcattaata ctaacataac tgctacccca gcgagcgtaa
    10561 tataagtttc tgtttcttgt ttaagtggtt tgcgacgaat ggcttctaaa atattcatca
    10621 ctatcttacc cccgtcaagg gctggtatag ggatgagatt cataatacct aagttgatag
    10681 agattaatcc cattaagttg agaactgtga cgaaaccatt tttagcagct tggctactag
    10741 cctgatagag agctactgga cctcccaatt tattaatact aaaatgagca ataagtttct
    10801 ttaattcatt taaaattcta aagaaacttt cccatgctag tttaaatcca cctaaaagct
    10861 tgtctttaaa gcttgtcttt agagctggca taatgccgat gatgtaggat ttatcaactt
    10921 tctgtggctt aacgttgatc gttttcacca cctctttgct cttaatttta agggcaagtt
    10981 tttggttttt ttctaaatga ctagttgact tttctactgc tgctgtcaac tgttcccagt
    11041 tagaaacttt gtgcgaccct atttgtaaaa tacggtcatt attttttaaa cctgcactag
    11101 cagcaggacc attttctgat acacggacct gattggttga taaatcttga acacctcctt
    11161 gaataaaagc aagtgcaata aagactacaa gacctaaaat aaaattattc atcggacctg
    11221 caaaattggt aattaagcgc ccccatacag aagcattctg atactgcaca tctaagggag
    11281 caatacgaat ttcagtacca tcctcctcaa tgatagtagc atcgtgatca acactatagg
    11341 tttttgtctc acttaagacc aatcctgtga tagttagttt atcttctaaa tcataagctg
    11401 ttacattgat tggtaaagaa gtattgtcta attgttttcc tgataaatta atacgagtta
    11461 caatgccttc tttatttaaa gtaaggctag ccggcgtccc tgtcttaatt tcagtcttat
    11521 catcacccca accagccata cggacataac cacctagcgg taatatacga atagtatagg
    11581 ttgttccttc tttatcaatg tgagaaaaga tttttggacc cataccaata gcaaattcac
    11641 gaacaagaat tcctgatttc tttgcaaaat agaaatgccc aaattcgtga acaactacaa
    11701 tgacaccaaa aataataata aaggttaaaa ttcctagcat tcaattcctc tctaaataca
    11761 atatattatt ttagaataag ccaaagaagt gcatgattgg gaaaacaaaa atcatactat
    11821 caaagcgatc caaaatcccc ccatgacccg gaatcaattt acccgaatct ttaacaccaa
    11881 aatgacgttt aatactactt tcaactaaat ctccaaattg accaaaaatg ctaaagatag
    11941 ccactaaaac taacatgact aggaagctat gaggcgcata gacggtctta tcaaaaagca
    12001 taaaaaagaa agctaccaca atggctgaag caattcctcc cagactccct tcaattgttt
    12061 tatttggaga cactgaaggt agtagtttgc gttgtccaaa ttgacgacca atcatataag
    12121 caccgatatc agtcgcccag acaataaata aagctaacaa caccttatct attccagcca
    12181 tacgagcact gactagattt tgaaagccaa tacccacata aaaactagac gcaattggaa
    12241 aggcagcatc ttcaaaactg taagaattac tgttcaaaac agtacctgcc aaaatcataa
    12301 aaataacaat accgtatgct gataaactag cgtcaactgg taaaaaggac aaataactat
    12361 ctaaaggaac tgttaaaaca aaggctccta tcatagctaa tgcaccttca aaggaaaata
    12421 tttctaaacg acgcatacga agtaattcag aaacaccaat catagctaaa agtccaacta
    12481 aaaattggaa aggcaaacca cccattacta aaaaagggat aaatatggca agcgcgacag
    12541 ctccccaaat aacacgttct ttcattttct ctcttctttc tatacactac caaatcgacg
    12601 atggcgttga ttataatcca caatggcctt atgtagttcg tcttttttaa aatctggcca
    12661 taaaacggga gtaaagtaaa attcactata agctgattgc caaggtaaaa agttactcaa
    12721 gcgtaactct ccactagtgc ggataatcaa atcagggtcc ctataaagat agggtaagct
    12781 atttgtcatt aaatgatttg ctactaaatc ctctgtaata tcgtcaggat tcaacttagc
    12841 ttccaaaaca tcctgagcaa tttccttaat ggcattagtg atttctgaac gtccgccata
    12901 atttagcgca aaattcaata cgagccccga attatgtttt gtcctcaaac aagcgcgttg
    12961 cattgcatcg tatgttgcct taggtaattt atgagtatct ccgatgacct gtacacgtac
    13021 attattttta tctaactctg gtacatattt atcaaaaaat tcaaccggaa ggttcataat
    13081 aaacttaact tcatcatctg gacgtgacca attttctgta gaaaaggcat agacagttag
    13141 tactttaaca cctaattctg aagctgctat tgtaacttct tgaagagcat ccatccccgc
    13201 cttatgtccc ataacgcgtg gttttaaacg tttttttgcc caacgtccgt taccatccat
    13261 gataatacca atatgtgctg gtattttctc caaaggcatt tctacttgtt tttttctttt
    13321 aaatataaac ataatgttcc ttaatttagc aatcgttttt cttatcagcc cttttaaaag
    13381 agctggaaat atcccatact ataacatttt accacatttt aacttatttt tgagtattaa
    13441 aatgccattt gtgacagcaa actaaaaaga ccagaacaat catctggtcc tacatatctt
    13501 attcagtttc gatggctgtt tctttatttt cgtcttcatt gacttcaata atcttaacat
    13561 cttcaactgg ttctgttggt gttgctgcct tagagacaac agatttgata gctgccaact
    13621 caaacgtcaa gtagacacca tcaacatcta aaaccattcg ttgtgcttca gtattaactt
    13681 catcaactac tccaaaaaga ccaccaattg tgacaatttc atcacctttt tgaacagcat
    13741 tcaattgttt ttgacgttct tgtgcttgtt tcttttgctg acgttgcatg aagaacatca
    13801 taccaaccat aacaacaagc ataataatag gtaagctcat ttgattctcc tttccgtgtt
    13861 ctacgtaatc attactatag cagatttatg ctatatctgc aaggagatac tgtctcttag
    13921 ccaataaatt cacggatttc ttcttttgtc atttttgaat cgcacttcac cttgatagtt
    13981 ccgtttttaa caaccatcaa acctggcact gtaggaatgt catatttatt tcgaaaatct
    14041 ttatttgcag tccaatgatc aaaatcttca ctatctaaat ggaataattc aaattcatct
    14101 tcatcagcta cttctacaat tttaggaaca aagaggtgac aaaatggaca agaaatacga
    14161 ccaatgaaca gtacaaaatc ttgatttgaa tcaattttat cttcaatttc ggcagccgtt
    14221 atcaaagtca tggttgccaa aagttcttca aaagaataca tagttaacca actttctaaa
    14281 tcttatattt aaagaataac ctatgatggc aaaacttgca aggcaagagt aacaacgatt
    14341 ctatcaaaat cattaactca cttctcgctt caaactatcc tcaaaaatgc tcactcatat
    14401 ttaaatgact catcaaaaaa actgagcata gctcagtttt tattttacac cttatttttg
    14461 tacagcatta ctaacgcttt tagcaactac atcggccaca tcattttgaa aagcatttgg
    14521 aatgatgtga gttgtgctta attcttcctc cggtattaaa ctagctatgc ctctcgctgc
    14581 cgcaatttgc atttcaacag ttatcgtttt tgctctagca tctaatgctc ctctgaaaat
    14641 acccggaaat gctaaaacat tattaatttg atttggaaaa tcactacgac cagttccaac
    14701 tatgtaagct ccggctttta aggcttgatc tggataaatt tctggaatag gattcgccat
    14761 tgcaaataca ataggtttat cagccatctt ggaaatccat tctgcatgta atgcttccgg
    14821 agcagaaaca cctataaaaa catcggcatt ctccaaagca tcttctaagg atccagattg
    14881 gaattctcta ttagtcaatt ttgcaatcgc tttgtgatga ggggctaatg actcccgatc
    14941 tttatcgtta ataatgccaa atcgatcaac taccgttaca tgttttgctc ctgctgatag
    15001 aagcttcctt gttatagata agcccgctga acctccacca ttaacaacta ctttgatatc
    15061 ttcaatgtct tttttaacta acttcaaact gttaaacaaa gccgctaata ctacgattgc
    15121 tgtaccatgt tggtcatcat ggaaaactgg tatatcacat tcttcaatta ggcgttgttc
    15181 aatttcgaaa caacgtggtg ctgaaatatc ctctaaatta atccctccaa aagtcggcga
    15241 aatcgctttt actatagaaa taatttcctc tgtatcattt gttttcaaca caattggcac
    15301 agcatctaca tttgcaaaac gtttaaagag tgctgcttta ccttccatga ctggcattgc
    15361 tgcctcagga caaatatctc ctaatccaag aacagctgaa ccatcactta ctacggctac
    15421 tgtatttttt ttagtcgtca actcatatgc aagctccaca tcctctgcaa tagccgatga
    15481 tactgacgcc acccctgggg tataggcaat actaagatcc ctcttatctt ccaccttaac
    15541 ttttgaaatt acttctaatt ttccaccatt ttctctagct tggttgatag ctaattgacc
    15601 taaattttca ctcattttga taactccttt tgataaatat tagaataaca tgcgtaaaat
    15661 ggctgtcatc gtgataacag taatagctcc tcctaaacgc gtagcaactt gagcaaatgg
    15721 catcaagttc attcggtctg ctgtacttaa aatggcaaca tctcctgttc ctcccatacc
    15781 actttgacaa gctgaaataa tacctgcttc aacaggattc atattaagga atcgcgatac
    15841 aaagaatcca acagaaataa ctgtaaatac aacactaata acaactatga aatattgtat
    15901 gctaagcgtt gccacaacat cttttaacgg gatataaagc aatcctagcc ctgccattag
    15961 aggaaatgta aaattaccag aaataaattt ataaagttgc ttagctccat tttgtgtttc
    16021 tctaggaata acattgatat atttcaaaat agctgctgcg ataatcatta atacgggacc
    16081 aggaaatcca gttaattgct gcaacaaatg tccaaaaata aagagactac atgctgttaa
    16141 aacacctcca cccattttct taacgtcaat tgggccagaa tgatcagcaa taatatctga
    16201 catgtcctct cctttattta accttactaa ttgctcttga ccagacaagt gtggtttctt
    16261 ttctcccaaa cgattcaata gtgcagtaca taaaatggca aagaaattac caataatagt
    16321 ggctgggatg agttgagcaa ctagttgttc gctagctaca ccggtaattg aactatagcc
    16381 taacgataat ggtaaaatac cttcaccaat accaccagct aaaacgggtg ttacgatata
    16441 aaacaaagtg tgtcgccact ctaagcctaa aataacaccg acaaatgtcc ctaccatcat
    16501 cgcacaaacc attcctaata acatggggaa aatcattctt agtaatccct gaatcaacat
    16561 tttccgattc atccctaaaa tactaccgga cactaaacaa gcaatataaa aataaagaaa
    16621 gttagcttgc ttcatcaaca tatttgttga ctctaaaaca tttttattaa tgaggttgaa
    16681 aaacaccaaa atagatggta ctaataaaga taaaatagct gggccaccga agtttttaaa
    16741 accagggatg ctagctccaa tagtacctaa gaaccatccc attgttaaga tgactgcaaa
    16801 tcctcctagc atattgactg gcaatttttg taaaaagcct gctagaagaa tcaataatgc
    16861 taaacaaaca tatactggta agggaactga gcctacctta atatgaaata gtttagtctt
    16921 taagtcttgc ttttgacctc tagaaccctc attattcact accttcacgt ctgccatgaa
    16981 agcacctcct tttacttctt atgtacagtt tactttggga cttagtgata aggaagttta
    17041 tttaagtaat tgaattattt caacttattt aattaattta agctggattc tagtttttag
    17101 gctatgttat actaaactcg gaggatgctg atgaaaaaaa aactatctct ttgggcattt
    17161 ctatcattga ttttagtcac tatgaccatt tgtatatttt ctatttttta ttatgtcact
    17221 atacatcagt cctatcgtat ggtaagggta caggaagaaa aaatcttaaa aaatactggt
    17281 tatgctttat cgagaaatcc acaagttata caaacactca aagataatca ctataatcaa
    17341 agtctccaaa aacaaatgct attccttagc aaaaaatcaa atttagacta tattgtcctt
    17401 ataaatttaa aagggatccg ttttacccac cctgactcca ctaagattgg taaaccattt
    17461 caaggcggtg atgaacaagc tgtttttaag ggaaaagcta ttatgtctac tgcagaaggt
    17521 tctttaggaa aatctttacg atacttaatt ccagtatatg atcaccaaaa acaggttggg
    17581 gcaattgcag ttggcttaaa attaacgaca cttggagact tatctcaaag cagcataaaa
    17641 gagtttagta agcctctctt gattagtatt ttgattagct tagtcgtcac aagtataatt
    17701 tcttatggct taaaaaaaca acttcacaac ctccatccaa gtgatatttt tcaacattta
    17761 gaagagcgca atgcaacact ggaccaaatt caagccgctg tatttgttat tgaccagagg
    17821 catatcatca aaagaaataa tcctgcagct agtttattat tcaaaaaaga ggggcaacga
    17881 gacctatttt caggtaaatt attagaatct ttaattcctc aactcaagca ggatcatttt
    17941 tcaaaaaaaa ctgaacaagt attacatttt caaggacaag actatttact atctatctcg
    18001 ccaattacgg ttaaaacaca aaatagaggc tatgttgtct tcttaaggaa tgttactgaa
    18061 acacttttca ctttggatca attagcacac actacagctt atgcttctgc tctacaggcc
    18121 caaacacatc aatttatgaa tcaacttcat gttatttatg gacttgcaga catcgaatac
    18181 tacgacgagt tgaaaattta tttaaaagaa ttattggagc cacaaaatga atttttagca
    18241 cgcttatcaa tgttagttag agagcctcga ctggctagtt tcattattgg cgaaagagaa
    18301 aaattcgcag aaaaacacat caatctatcc acagaaattt tagtagaaat ccctaccaag
    18361 tcaactgttg aggatgttaa taactatctc ttgctccaca gatatattaa tacgaagata
    18421 ctcacgctat taaattcaac gactttagtt tctttacgtt taaattatca aaataacctc
    18481 attgaaacag attatcaatg ggagaatgag aagtggttac tcaatgacta ccatcagtat
    18541 ttcaacgacg cttattttca acagttactt gtagattcaa gagctactta taaga
//
LOCUS       DATRQA010000029        18106 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.029, whole genome shotgun sequence.
ACCESSION   DATRQA010000029 DATRQA010000000
VERSION     DATRQA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18106
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.029"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(298..2880)
                     /locus_tag="VBD27_000720"
     CDS             complement(298..2880)
                     /locus_tag="VBD27_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7024750.1"
                     /translation="MNRKKTVIISALSVALFGTGVGAYQLGSYNAQKSDNSVSYVKTD
                     KSDSKAQATAANKTPDQISKEEGISAEQIVVKITDDGYVTSHGDHYHYYNGKVPYDAI
                     ISEELIMKDPSYVFNKADVINEVKDGYIIKVNGKYYLYLKEGSKRTNVRTKEQIQKQR
                     EEWSKGGSKGESGKHSSAKTQALSASVREAKASGRYTTDDGYVFSPTDVIDDMGDAFL
                     VPHGDHFHYIPKADLSPSELSAAQAYWNRKTGRSGNSSKPSNSSSYIHASAPSGNVST
                     GRHANAPISIPRVTHANHWSKPAGNHATAPKHHAPTTKPIKKDSALDKMLKRLYAQPL
                     SARHVESDGLVYDPAQVNAFTAIGVSIPHGNHFHFIHYKDMSPLELEATRMVAEHRGH
                     HIDALGKKDSTEKPKHISHEPNKEPHTEEEHHAVTPKDQRKGKPNSQIVYSAQEIEEA
                     KKAGKYTTSDGYIFDAKDIKKDTGTGYVIPHMTHEHWVPKKDLSESELKAAQEFLSGK
                     SEANQDKPKTGKTAQEIYEAIEPKAIVKPEDLLFGIAQATDYKNGTFVIPHKDHYHYV
                     ELKWFDEEKDLLADSDKTYSLEDYLATAKYYMMHPEKRPKVEGWGKDAEIYKEKDSNK
                     ADKPSPAPTDNKSTSNSSDKNLSAAEVFKQAKPEKIVPLDKIAAHMAYAVGFEDDQLI
                     VPHHDHYHNVPMAWFDKGGLWKAPEGYTLQQLFSTIKYYMEHPNELPKEKGWGHDSDH
                     NKGSNKDNKAKNYAPDEEPEDSGKVTHNYGFYDVNKGSDEEEPEKQEDESELDEYELG
                     MAQNAKKYGMDRQSFEKQLIQLSNKYSVSFESFNYINGNQVQVTKKDGSKVLVDIKTL
                     AEVK"
     gene            complement(2907..3830)
                     /locus_tag="VBD27_000721"
     CDS             complement(2907..3830)
                     /locus_tag="VBD27_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEO7024751.1"
                     /translation="MKRIRKSLIFVLGIVTLICLCACTKQSQQKNGLSVVTSFYPVYS
                     ITKAVSGDLNDIKMIRSQSGIHGFEPSSSDVAAIYDADLFLYHSHTLEAWARRLEPSL
                     HHSKVSVIEASKGMTLDKVHGLEDVEAEKGVDESTLYDPHTWNDPVKVSEEAQLIATQ
                     LAKKDPKNAKVYQKNADQFSDKAMAIAEKYKPKFKAAKSKYFVTSHTAFSYLAKRYGL
                     TQLGIAGVSTEQEPSAKKLAEIQEFVKTYKVKTIFVEEGVSPKLAQAVASATRVKIAS
                     LSPLEAVPKNNKDYLENLETNLKVLVKSLNQ"
     gene            complement(4085..4528)
                     /locus_tag="VBD27_000722"
     CDS             complement(4085..4528)
                     /locus_tag="VBD27_000722"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO7024752.1"
                     /translation="MKIIIQRVSQASVSIEDDVVGSIEKGLVLLVGIAPEDTTEDIAY
                     AVRKITSMRIFSDDEGKMNLSIQDIKGSVLSISQFTLFADTKKGNRPAFTGAADPVKA
                     NQFYDIFNQELANHVSVETGQFGADMQVSLINDGPVTIVLDTKNK"
     gene            complement(4538..6754)
                     /locus_tag="VBD27_000723"
     CDS             complement(4538..6754)
                     /locus_tag="VBD27_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO7024753.1"
                     /translation="MVKEINLTGEEVVAITSQYMSETDVAFVKFALNYATAAHYYQAR
                     KSGEPYIIHPIQVAGILADLHLDAVTVACGFLHDVVEDTEITLDEIETDFGKDVRDII
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMSEKRREREELV
                     DIIVDKIRSYTEEQGLYGDIYGRPKHIYSIYRKMRDKKKRFDQIYDLIAIRCIMETAS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEFGVAAHWAYKKGITSKVNQAEQSVGMGWIQELVELQDESKDAKDFVDSVKEDIFTE
                     RIYVFTPNGAVQELPRESGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVVE
                     IITNPNSFGPSRDWIKIVKTNKARNKIRQFFKNQDKETSINKGRELLVDYFQEQGYVP
                     NKYLDKKHIEEILPRVSVKSEEALYAAVGFGDLSPISIFNKLTEKERREEERAKAKAE
                     ADELINGGEIKTDKRDVLKVKSENGVIIQGASGLLMRIAKCCNPVPGDLIEGYITKGR
                     GVAIHRSDCQNLKSQENYEQRLIDVEWDDDGSKKEYMAEIDIYGLNRSGLLNDVLQTL
                     SNATKLVSTVNAQPTKDMKFANIHVSFGISNLAQLTTVVDKIKIIPDVYSVKRTNG"
     gene            7014..9416
                     /locus_tag="VBD27_000724"
     CDS             7014..9416
                     /locus_tag="VBD27_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEO7024754.1"
                     /translation="MSKHYFSKSIFALTVLTATTTSGLAVQAEDIVTTPSSTATKVES
                     TTPTSTIAEEKSNVTSTPTAITDASTATSTNTTANNQNPQPVATETTTGDLKPIEGEK
                     VDLRIMSTTDLHTNLVNYDYYQDKESQKIGLAKTAVLIEEAKKENPNTVLVDNGDVIQ
                     GTPLGTYKAIVKPVAENEEHPMYQAMNALGYDASTLGNHEFNYGLDYLKKIIATANLP
                     ILNANVLDFKTHQPVFKTYDIITKTFKDSTGRAVALNIGITGIVPPQILNWDKANLEG
                     KVIVKDSVKAIEEIVPTMRAKGADVILVLSHSGIGDDRYEEGEENVGYQIASIKGVDA
                     VVTGHSHAEFPSGNGTGFYEKYTGVDGINGKINGTPVTMAGKYGDHLGIIDLGLSYTN
                     GKWQVSESSAKIRKIDMNSTTADERIIALAKEAHDGTINYVRQQVGTTTAPITSYFAL
                     VKDDPSVQIVNNAQRWYVANELKGTPEANLPLLSAAAPFKAGTRGDATAYTDIPAGPV
                     AIKNVADLYLYDNVTALLKVTGADLREWLEMSAGQFNQIDPNNKAPQNIINTEYRTYN
                     FDVIDGLTYKFDITQPNKYNKDGKVVNSQASRVRDLMYNGKPVADKQEFMIVTNNYRA
                     SGTFPGAKNATMNRLLNLENRQTIINYIISEKTINPTADNNWGFTESIKGLDLRFQTA
                     DKAKNLVTNSKDIQYIASSTKDEGFGDYRFVYTEQEKVDLTEPSHQEKLSQKEKQKIH
                     HMVATAYTKAKASPNIAGTIYTQQKEKTLPKTGQEKQSYLAFFGVAILGLVGNLLKKK
                     QI"
     gene            9573..10028
                     /gene="nrdI"
                     /locus_tag="VBD27_000725"
     CDS             9573..10028
                     /gene="nrdI"
                     /locus_tag="VBD27_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO7024755.1"
                     /translation="MPKLTLVYISLSGNTQSFVKRLSEQLSTWHNCQVSTINIKEQNH
                     QTFPVDQPFVALLPTYLEGGNGIDNGDQEILTIPLGDFIAAHNNVKNCIGIVGSGNRN
                     FNNQYCLTAKQYSERFGFPMLGDFELRGTSSDVERIANIIVETLSNFKA"
     gene            10039..11076
                     /locus_tag="VBD27_000726"
     CDS             10039..11076
                     /locus_tag="VBD27_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO7024756.1"
                     /translation="METTLNYIKTLTSIPSPTGFTQTIMTYIIKELEAFGYSPIRTNK
                     GGVMVSLKGKNNTKHRMITAHLDTLGAMVRAIKPDGRLKIDLIGGYTYNAIEGENCTI
                     HLSKNGKEISGTALIHQTSVHVYKDAGTAERNQTNMEIRLDEKVTTADETRALGIQVG
                     DFISFDPRTIITGSGFIKSRYLDDKVSAGILMELLSVYKKEDIQLPYTTHFYFSAFEE
                     LGHGANSSIPNETVEYLAVDMGAMGDDQETDEYTVSICVKDASGSYHYELRQHLVSLA
                     ENNNIPYKLDIYPYYGSDASAAMRAGAEVKHALLGAGIESSHSYERTHIDSIQATELL
                     VDAYLKSNMVN"
     gene            11076..11573
                     /locus_tag="VBD27_000727"
     CDS             11076..11573
                     /locus_tag="VBD27_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO7024757.1"
                     /translation="MVATLIIIRGNSASGKSTIAKQLQAELGEDTLLLSQDYLRREML
                     GTKDGENTTTIPLLINLLNYGYHNCSYIILEGILRSDWYTPVWKHILKHNPNNTYAYY
                     YDLSFQETVKRHSTRLKSLEFGEDSLARWWLEKDFLKEIPEKILTKSMSLEDVKKLIM
                     TDVLP"
     gene            complement(11795..12832)
                     /locus_tag="VBD27_000728"
     CDS             complement(11795..12832)
                     /locus_tag="VBD27_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO7024758.1"
                     /translation="MKEKQSKRLIYILLIVPIIFISVFTYSISQPSKLLPPKELVILS
                     PNSQAILTGTIPAFEEKYGIKVKLIQGGTGQLIDRLSKEGKQLKADIFFGGNYTQFES
                     HKALFESYVSKNVHTVIPDYIHPSDTATPYTINGSVLIVNNELAKGLTIKSYEDLLQP
                     SLKGKIAFADPNTSSSAFSQLTNILLAKGGYTNPKAWNYVKKLQHNINAIKSSSSSEV
                     YQSVAEGKMIVGLTYEDPSVNLQKSGANVSIVYPTEGTVFVPSSVAIIKNAPSMKEAK
                     LFINFMLSLDVQNAFGQSTSNRPIRKDAQTSNGMKALKDIATLKEDYRYVTKHKSQIL
                     KTYNRIRRNAD"
     gene            complement(12844..13617)
                     /locus_tag="VBD27_000729"
     CDS             complement(12844..13617)
                     /locus_tag="VBD27_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO7024759.1"
                     /translation="MYRLLIVEDEHLIRKWLRYAIDYQSLNILVVGEAKDGKEGAQLI
                     QEEQPDIVLSDINMPIMTAFDMFEATKGQSYAKIILSGYADFPNAQSAIHYGVLEFLT
                     KPLEKQALIDCLKTIMARIEEHKEKHLQEHTELYLPLPQANDQVPEVIKDMLAWIHSH
                     FHGKIVISQLAHDLGYSESYLYTVTKKHLHITLSDYINQYRINQAIQLMFREPDLMVY
                     QIAEAVGIYDYRYFDRVFKKYLGQTVKAFKEEHIFKQMD"
     gene            complement(13621..15270)
                     /locus_tag="VBD27_000730"
     CDS             complement(13621..15270)
                     /locus_tag="VBD27_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7024760.1"
                     /translation="MRGYRMEERFKKRLQDDISKHFSRQSLILSLLLIALFVLFSLAP
                     QQIGLYKDVNSVSYSYKQLIQKHDTLLDDLGKNSLKPFVSGHLGSADLSKQYYHLRNH
                     LQSQTELLVFSPNQELLFASNSHLGNFFSKSIYISEVLDKAKINQRLLKIIVDSEGGH
                     YLALIKPIIVNKKVSGYAFLLMNGKDFLLPTKAINSDLIIADQLNNSFTFTNRDFISS
                     SLDKVDSQFLTRYFSFHDHRAFVVRKVALQDNILLYMYRPLIPVTLVVLFSLVSSVII
                     FVILRRKSRVLADRIAVKNSGAINQMVLDMDAISRQEKSSIELDSQDEFQYLSVQINQ
                     MVSRLKDLHEKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSQLTERIV
                     IQLTKLLRYSLSGSTEAAVLKDDLAIIESYLLINQVRFEELTYTISVSPELEHMRVPK
                     LFLLPLIENAIKYGLKERHDVAINIDIWQDSDGIWFTVSNNGSGISLARQKAIRTMLR
                     SNHSHHGLINSYRRLQYQFSTVLLEFTKTDDAFRVSYIVKE"
     gene            complement(15408..16235)
                     /locus_tag="VBD27_000731"
     CDS             complement(15408..16235)
                     /locus_tag="VBD27_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7024761.1"
                     /translation="MKSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASTGSAMGAVVFLLLYNIPAFLIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQSYLDQLFVGVVPLLVTILAFWLLRKKVNINWIMFGIMVLGIVLGLLGIC"
     gene            complement(16232..17041)
                     /locus_tag="VBD27_000732"
     CDS             complement(16232..17041)
                     /locus_tag="VBD27_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO7024762.1"
                     /translation="MIMPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGMLLGDI
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISNIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNSFVHHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDIELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            complement(17045..17548)
                     /locus_tag="VBD27_000733"
     CDS             complement(17045..17548)
                     /locus_tag="VBD27_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7024763.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEESIALFKDPRATDKRIFVIVNSVKDACTIAKNITDLEAVN
                     VANVGRFDKSDPATKVKLTSSLLLNPEELEAAKELASLPDLDVFNQVLPSNTKVNLSQ
                     LVKQSEV"
     gene            complement(17566..17991)
                     /locus_tag="VBD27_000734"
     CDS             complement(17566..17991)
                     /locus_tag="VBD27_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEO7024764.1"
                     /translation="MKRKFLIGSHGKLASGLQSSIDILTGKGQEIQTIDAYIDDSDYT
                     KSIVEFIDEIAPDEQGLIFTDLLGGSVNQKMATAVMNSGKNNVFLITNSNLATLLSLL
                     FLKPEEELTKEEIVTVINESQVQLVDLSFKACSEDDFFD"
BASE COUNT         5723 a         3552 c         2964 g         5867 t
ORIGIN      
        1 ataacaatta catgtaataa aacaagtgag ccactactag aaacctgtta tacagctttt
       61 agtagtggct ctttatttag tgattataac tttagtcgtt ttataaaagt caaaacaaga
      121 aaacttcctc aaacaaaaat acattaactc tcttttaagc gtaaaacaat ggttgttcaa
      181 aagaagaatt agatatatca ttaaaatctt cgcacgctag aaatcataac taaaaaacaa
      241 acaagtgcgg tgacttgttt gtttttagtt gtcagctaag tttattcaac tcttttatta
      301 tttcacttct gctagtgttt taatatcaac aagcacttta gagccatctt ttttagtaac
      361 ttgaacttga ttaccattaa tataattaaa gctttcaaaa cttacactat atttatttga
      421 taattggatg agttgctttt caaaagattg tctatccata ccatatttct tagcgttttg
      481 tgccattcct agttcatatt catctagctc tgattcatct tcttgttttt ctggttcttc
      541 ttcgtctgaa cctttattaa catcataaaa accatagttg tgagttactt tccctgaatc
      601 ttcaggttct tcatctggag cataattttt ggctttattg tctttatttg agcctttgtt
      661 atgatcactg tcgtgtcccc aacccttttc ttttggtaat tcattaggat gttccatgta
      721 gtatttaatt gttgagaaga gttgttgtaa tgtatagcct tctggtgctt tccataaacc
      781 acccttgtca aaccatgcca taggaacatt atgataatga tcatgatgag gaacaatcaa
      841 ttgatcatct tcaaatccaa ctgcatatgc catgtgagca gcaattttat caagcggtac
      901 aattttttct ggttttgctt gtttgaatac ttctgcagca cttaagtttt tgtcactaga
      961 atttgatgtt gatttattat cagttggtgc aggacttggt ttatctgctt tattagagtc
     1021 cttttcctta taaatttcag catctttacc ccatccttca actttaggac gtttttctgg
     1081 gtgcatcatg taatatttag ccgtagctaa atagtcttct aaagaatatg tcttatctga
     1141 atcagctaaa agatcttttt cttcatcaaa ccattttaat tccacataat ggtaatgatc
     1201 tttatgagga attacaaatg taccattctt atagtctgtc gcttgtgcaa ttccaaataa
     1261 taaatcttca ggtttaacaa ttgcttttgg ttcaattgcc tcatagattt cttgagctgt
     1321 tttacctgtt tttggtttgt cttgatttgc ttcagatttt cctgaaagaa attcttgagc
     1381 tgcttttaat tccgactctg ataaatcttt ctttggtacc caatgctcat gtgtcatatg
     1441 tggaatgaca taacctgtac ctgtatcttt tttaatatct ttagcatcaa aaatgtaacc
     1501 atcagatgtt gtgtatttac cagctttttt tgcctcttca atttcttgag cactgtagac
     1561 aatctggcta tttggtttgc ctttacgttg gtctttcggt gttactgcat ggtgttcttc
     1621 ctctgtgtga ggttccttat taggttcatg agaaatatgc tttggtttct ctgtagaatc
     1681 ttttttccct aatgcatcaa tatgatgtcc tctatgctct gccaccatcc ttgttgcttc
     1741 taactctaat ggagacatat ccttatagtg aataaagtgg aaatgattac cgtgcggaat
     1801 agaaacaccg attgctgtga aagcattaac ttgtgctggg tcataaacca aaccatcact
     1861 ttctacgtga cgagcagata acggttgagc atataaacgt ttcaacattt tgtctaaagc
     1921 agaatctttt ttgattggtt tggttgtagg tgcatggtgt tttggtgccg tagcatgatt
     1981 accagctggt tttgaccagt gatttgcgtg agtgactctt ggaatagaaa taggggcatt
     2041 agcatgtctt cctgttgata cattaccgct tggtgcactt gcatgaatgt atgaacttga
     2101 atttgatggt tttgagctat tacctgatcg gccagtcttg cggttccaat atgcctgtgc
     2161 ggctgataac tctgatggag ataaatcagc tttcgggata taatggaaat gatcgccatg
     2221 tggtactaaa aaggcatctc ccatgtcatc aataacatct gttggactaa aaacataacc
     2281 atcatctgta gtatagcgac cgctagcttt cgcttcacgt acgctggctg ataaagcttg
     2341 agtttttgca ctactatgct ttcctgattc tcccttagaa ccacccttag accattcttc
     2401 gcgttgcttt tggatttgtt cctttgtacg aacatttgtt cgtttgctac cttctttaag
     2461 atagaggtaa tacttaccat taactttgat aatataacca tctttgactt cattaatgac
     2521 atcagcttta ttaaagacat agctagggtc tttcataatc aattcttcac taatgatggc
     2581 atcatatggc actttcccat tgtaataatg gtaatggtct ccatgagaag ttacataacc
     2641 atcatcagta attttaacaa cgatttgttc tgcagatatg ccttcttctt tactgatttg
     2701 gtcgggcgtt ttatttgcag ctgttgcttg tgctttgcta tcagatttat ctgttttcac
     2761 ataagaaaca ctattgtctg atttttgggc attatagcta cctaattggt aagcacctac
     2821 accagtacca aatagagcta ctgataaagc actaataata actgtttttt tacgattcat
     2881 cctgcgtcct ttctttatat aactacctat tgatttaacg atttgacaag taccttaaga
     2941 ttagtttcca aattttctaa gtaatcttta ttgtttttgg gaactgcttc taaaggactt
     3001 aaacttgcaa ttttaactcg agtagctgaa gctactgctt gagctaattt aggtgagact
     3061 ccttcttcaa caaaaatagt cttaacctta tatgttttca caaactcctg aatttcggct
     3121 aattttttag cactaggttc ttgctcggtt gagacacctg caatacctaa ctgagtcaat
     3181 ccgtatcgct tagctaagta tgagaatgct gtatgtgaag tcacaaagta tttagacttt
     3241 gcagctttaa attttggctt atacttctct gcaatagcca ttgccttgtc actaaattga
     3301 tcagcatttt tttgataaac cttagcgttt ttaggatcct ttttagctag ttgtgtagcg
     3361 atgagttgtg cttcctcaga tacttttaca gggtcattcc aagtgtgagg gtcatacaag
     3421 gttgactcat ctactccttt ttctgcctct acatcttcta agccatgaac tttatccaaa
     3481 gtcatacctt ttgaagcttc aattacagat actttagagt gatgcaaact aggttccaaa
     3541 cgtctcgccc aagcttctag tgtgtgcgaa tgataaagaa atagatcagc atcataaatg
     3601 gcagcaacat cacttgatga gggttcaaaa ccatgaatgc ctgactgtga tcgaatcatt
     3661 ttaatatcat tcaaatcacc agaaactgct tttgtaatgg aatatactgg ataaaagcta
     3721 gtcacgaccg acaagccatt tttttgctgg ctctgtttag tacaagcaca taagcaaatt
     3781 agggtaacta ttccgagaac aaaaataagg cttttccgta tccttttcat taaacctcct
     3841 ttaaaataaa aagtacttaa ccagttaaca ttttagagta ttcttatttt agtgtcaacg
     3901 aaaaaccata aatctaatat ttttctattt ttaatattgt taagtgcact taatttcata
     3961 tcaacaacct ttattcatgc ctttccaagc tacagtgaat ctttttataa acatgaatgg
     4021 gttaaggagt ataattagaa ccacaataaa aagagccaag cattttgccc aactctttga
     4081 ttttttattt atttttagta tctaaaacga tagttactgg tccatcatta atcagactga
     4141 cttgcatatc agctccaaat tgacctgttt ctaccgaaac atgatttgct aattcttgat
     4201 taaagatatc ataaaactga ttcgctttaa caggatcagc tgccccagta aaagctggac
     4261 gatttccttt tttggtatct gcaaagagtg taaattgtga gatagataag acgcttcctt
     4321 taatgtcttg aattgaaagg ttcattttcc cctcatcatc agaaaaaata cgcatgctag
     4381 taattttacg aactgcataa gcaatatctt ctgttgtatc ttctggagca atcccaacta
     4441 gaagtactaa acccttctca atagatccga ctacatcatc ttcaatagaa acacttgctt
     4501 gactaacacg ttgaataatg attttcattt tgctctccta accattcgta cgtttaacag
     4561 aataaacatc tgggataatt ttgattttat caaccacagt tgtcaattgt gctaaatttg
     4621 aaattccaaa gctaacatga atattagcaa atttcatatc ttttgttggt tgtgcattta
     4681 ctgtcgatac tagctttgtt gcatttgata aggtttgaag aacatcatta aggaggccac
     4741 tacgattcaa accataaata tcgatttcag ccatgtactc ttttttagat ccatcatcat
     4801 cccactcaac atcaatcaaa cgttgttcgt agttctcctg actctttaaa ttttgacaat
     4861 ctgaacgatg gatagccacg cctctacctt tagttatata gccttcaatg agatctccag
     4921 gaacgggatt acaacatttt gcaatacgca ttaataaacc tgaagctcct tgaatgataa
     4981 caccattttc actcttaact ttgagaacat cacgcttatc ggttttgatt tcgccaccgt
     5041 taataagttc gtctgcttcc gctttagcct tagcacgttc ctcctcacgg cgttcttttt
     5101 ccgtcagttt attaaaaata ctaatcggac tcaaatcacc aaaacctacg gctgcataca
     5161 aagcttcctc acttttaaca ctgacacgtg gaaggatttc ttcaatatgt ttcttatcta
     5221 aatatttgtt aggcacataa ccttgttctt ggaaataatc aaccagtaat tctctacctt
     5281 tattaataga agtttcttta tcttggtttt tgaagaactg acgaatttta ttgcgagctt
     5341 tattggtctt aacaatctta atccagtcac gacttggtcc aaatgaatta gggtttgtga
     5401 taatctcaac aacatctcct gtttttaact tagcagtcaa tggaaccata cgtccattta
     5461 ctttagcgcc agtagctttt tctccaactt gcgtgtgaat agcataagca aaatcaattg
     5521 gtccagattc ccttggcaat tcctgaacag caccatttgg ggtaaaaaca tagatgcgtt
     5581 ctgtaaagat atcttcctta acagagtcaa caaaatcctt ggcgtctttt gattcatctt
     5641 gaagctcaac taattcttga atccatccca taccaaccga ttgctctgct tgattaacct
     5701 tactggtgat tcctttttta taggcccaat gggctgcaac accaaactcg gcaacttgat
     5761 gcatttcttt agttcgaatc tgaatttcaa ttggcccttt aggtccataa accgtcgtgt
     5821 gaatagattg ataaccattg gcctttggtg ccgcaatata atctttaaac cttcctggca
     5881 tcggacgcca taactcatgg atatacccca ccatggcata aacatcacta gcagtttcca
     5941 taatacagcg tatcgcaatt aaatcataaa tctgatcaaa gcgtttcttt ttatcacgca
     6001 tttttctgta aatagaataa atgtgttttg gtctaccata aatatcacca tataaaccct
     6061 gttcctcagt ataggatctg attttatcga caattatatc aaccaactct tcgcgttcac
     6121 gacgtttttc actcatcata tgagaaatct tataaaattc cgtctcattc aagtaacgaa
     6181 aagataaatc ttctaattcc cacttgatac gactaatccc caaacgatgc gctaaaggag
     6241 cgtatatttc catagtctca cgtgaaatac gctcttgttt atcttttctg aggtgtttaa
     6301 gggttctcat attatgtaag cgatcagcga gcttaaccaa aataacacgg atatccttag
     6361 acatagccat taacatttta cggtgatttt ctgctaactg ttcttcatga gatttgtact
     6421 ccactttacc caattttgtt acaccgtcaa taatatcgcg aacatccttg ccaaaatcag
     6481 tttcaatctc atcaagggta atttccgtat cttctacaac atcatgtaaa aaaccacaag
     6541 ctaccgtaac ggcatcaagg tgtaaatctg caagaatacc tgctacttga attggatgaa
     6601 taatgtaggg ctcgcccgac tttcttgctt gataataatg agctgcggtt gcgtaattta
     6661 aggcaaattt tacaaaagca acatccgtct cactcatgta ctgggatgtg attgcaacaa
     6721 cctcttctcc tgttaaatta atttctttga ccatagttat cctctttaat catagtgata
     6781 gattgtacct attttatcac tttttaacct aaaaataaaa gaagttagag gctcaaaaag
     6841 aattaaatac caagtaaatg tgtctaacca agttatcctt tataaataaa cgattgtttt
     6901 tagtgtaatt actaaaaata attcgtaaat gagattgatt aaacgttttc aataagctat
     6961 aatagttttt agattaggat aatatacatg taatgtttta atggaggaga tttatgtcaa
     7021 aacattattt ttcaaaaagc atattcgctt taactgtctt aacagctaca acaacaagtg
     7081 gtttagctgt tcaagcagaa gacattgtta caacaccaag ttcaaccgct acaaaggttg
     7141 aatcaactac ccccactagc acaattgcag aggaaaagtc aaacgttact tctactccaa
     7201 ctgcaataac tgatgcttca acagccacaa gcacaaatac cactgccaat aatcaaaatc
     7261 cacagcctgt tgccacagaa accacaactg gtgatctcaa acctattgaa ggtgaaaaag
     7321 ttgaccttcg catcatgtca accacagacc tacacaccaa tttagttaac tatgattatt
     7381 atcaagataa agaatctcaa aaaatagggt tagcaaaaac tgctgtcctt atcgaagaag
     7441 caaaaaaaga aaatcctaat accgtcttag ttgataacgg agatgtcatt caagggacac
     7501 ctttaggaac ttataaagca atcgttaaac ctgtcgcaga aaatgaagaa catcctatgt
     7561 accaagccat gaatgctctt ggttatgatg catcaacact aggtaatcat gaatttaatt
     7621 acggtttaga ttacctcaaa aaaatcattg ctacagccaa ccttccaatc ctcaacgcaa
     7681 atgttttgga tttcaaaaca catcaaccag tttttaaaac ttatgacatc attactaaaa
     7741 ccttcaaaga ttcaacagga agagctgttg cactaaatat tggaattaca ggaattgtac
     7801 ctcctcaaat cttaaactgg gacaaggcta acttagaggg aaaggttatt gtcaaagatt
     7861 cagttaaagc aatcgaagaa atcgttccta ctatgcgtgc taaaggagca gatgtcattc
     7921 tcgttttatc acactctggc attggagatg atcgatatga agaaggtgaa gaaaacgttg
     7981 gctatcaaat tgccagcatc aagggagtgg atgccgttgt tacgggacac tcacacgctg
     8041 aatttccatc aggtaacggt actggcttct atgaaaaata cactggagtt gatggtatca
     8101 atggaaaaat aaatggaaca cctgttacaa tggcaggcaa gtacggggat caccttggta
     8161 ttattgattt aggacttagt tatactaatg gaaaatggca agtctccgaa agcagtgcta
     8221 aaatccgtaa aattgatatg aactcaacaa ctgctgacga gcgtatcatt gcattggcta
     8281 aggaagcaca cgatggcact atcaactatg ttcgccaaca agtaggtaca acaactgcgc
     8341 caattacaag ttactttgca ctagttaaag atgacccttc agttcaaatt gttaacaacg
     8401 ctcaacgttg gtacgttgca aatgaattaa aaggaacacc tgaggcaaat ttaccacttc
     8461 tatctgctgc agcaccattt aaagccggaa cacgtggcga tgctaccgca tatactgata
     8521 ttccagctgg acctgttgca attaaaaacg tagctgatct ttacctttat gataatgtta
     8581 ctgcactttt aaaagtgacc ggagcagact tacgtgaatg gttagaaatg tccgctggtc
     8641 aatttaacca aattgaccct aacaataaag caccgcaaaa tattatcaat accgaatatc
     8701 gtacctataa ttttgacgtt attgatggct tgacttataa atttgatatt acacaaccta
     8761 ataaatacaa caaagatgga aaagttgtta atagtcaagc tagtcgagta cgcgatttaa
     8821 tgtacaatgg taagcctgtt gcagacaaac aagagtttat gattgtgacc aataactacc
     8881 gtgctagtgg taccttccct ggggctaaaa atgccacaat gaaccgtttg ctgaacttag
     8941 aaaatcgcca aactattata aactatatta tttcagagaa aaccatcaat cctacagctg
     9001 ataataactg gggcttcaca gagtctatta aaggccttga tttacgattc caaacagccg
     9061 ataaagctaa aaatcttgtt actaatagca aggatattca gtatatcgct agttctacca
     9121 aagatgaagg atttggtgat tatcgatttg tctatactga acaagaaaaa gtagacctta
     9181 ctgagccatc acatcaagaa aagttaagcc aaaaagagaa acaaaaaatt caccacatgg
     9241 ttgcaactgc ttatacaaaa gcaaaagctt ctccaaatat cgcaggcact atctatacac
     9301 aacagaaaga aaaaacatta cctaaaactg gacaagaaaa acaatcttat ctggctttct
     9361 ttggcgtcgc aatacttgga ctcgtgggaa atttacttaa aaagaaacaa atatagtcat
     9421 cactaaaaac tgaggccaat caacctcagt ttttagtctc aacaatagat tttctctcta
     9481 aacagtcaga tattgagata ccactctagc ttgtttatct gacatgattt tgctaaactg
     9541 ataacaagaa atttagattt aaggagtact aaatgcctaa attaacccta gtctatatca
     9601 gtcttagcgg caatactcaa agctttgtca aacgtcttag tgaacaactg tcaacttggc
     9661 ataactgtca ggtttcgact attaatatca aggaacaaaa tcatcaaacc tttcctgttg
     9721 accaaccttt cgtagcacta ttacccactt acctcgaggg aggaaatggc attgacaatg
     9781 gcgatcaaga aattcttacc attccacttg gtgattttat agctgcacac aataatgtca
     9841 aaaattgtat cggaattgtt ggctctggaa atagaaattt taacaatcag tactgtttaa
     9901 ctgcaaaaca atactctgaa cggtttggat ttcctatgtt aggagacttt gaattgcgag
     9961 gaacttctag cgacgttgaa cgtatcgcaa atattatcgt tgaaacatta tcgaacttta
    10021 aagcctaaag gaaatagtat ggaaacaaca ttaaactaca ttaaaaccct gacatcaatc
    10081 ccttcaccaa cagggtttac ccaaacaatc atgacctata tcatcaaaga attggaagcg
    10141 tttggctact caccaattcg cacaaacaag ggaggcgtca tggtttctct aaaaggaaaa
    10201 aataatacta aacatcgcat gataactgct caccttgata cacttggtgc tatggttagg
    10261 gccatcaaac cagatggtcg gttaaaaatt gaccttattg gcggatacac atacaatgcc
    10321 attgaaggag aaaactgtac tatacacctc tcaaaaaatg gtaaagaaat ttctggaact
    10381 gctcttattc atcaaactag tgtccatgtt tacaaagatg ctggaactgc tgaacgtaat
    10441 caaacaaata tggaaattcg tttagatgag aaagtaacaa ctgctgacga aacacgtgct
    10501 ttaggcatcc aggtcggtga tttcatttca tttgatccgc gtacaatcat aacaggcagc
    10561 ggctttatta aatcacgtta cctagatgac aaggtatccg ctggtatcct aatggaactt
    10621 ctttctgttt acaagaaaga agacattcaa cttccttata ctactcattt ctactttagt
    10681 gcctttgaag agctaggaca tggagcaaat tcaagcatcc caaatgaaac tgtagaatat
    10741 ctagcagttg atatgggagc tatgggagac gatcaagaaa ctgacgaata tactgtctct
    10801 atctgtgtta aagatgcttc tggttcttat cattatgaat tacgtcaaca tcttgtttct
    10861 ctagctgaaa acaataatat tccttataaa cttgatattt atccttatta tggtagtgac
    10921 gcctccgctg ccatgcgtgc tggtgcggaa gttaaacacg cgctacttgg tgcaggtatt
    10981 gaatctagtc attcttatga acgtacccat atcgattcta ttcaagcaac tgaactctta
    11041 gtggatgcct atctcaaaag caatatggtg aactaatggt agccacgctc attattatca
    11101 ggggaaattc agctagcgga aagtctacaa tagctaaaca acttcaagcc gaattagggg
    11161 aagataccct tctcttatct caggattacc ttcgtagaga gatgttagga actaaggatg
    11221 gtgaaaatac tactaccatt ccccttttga tcaatttact aaattatggt taccataact
    11281 gttcctacat catccttgaa ggcatacttc gttctgattg gtatacgcct gtttggaaac
    11341 atattttaaa acataacccc aacaatactt atgcttatta ctatgattta agctttcaag
    11401 aaactgttaa aagacattca actcgactta aatctttaga atttggagaa gattctcttg
    11461 caaggtggtg gcttgagaaa gacttcttaa aagagatacc tgaaaaaata ttaacaaaat
    11521 ctatgagttt agaggatgtc aaaaaactta taatgaccga cgttttacct taacattatt
    11581 gtacgcacta taaacaataa tgacttctta tcattggagt atattgccca tggatacagt
    11641 gattgacctc ttatttatta tcgttcctat tatagccgga tttctggcat gcattagtct
    11701 ttatcgttat tggaaaaaag atttcgaaag cgacgaggac gatgattaaa ctcctcggaa
    11761 tcaatgattc cgaggagttt ttctatattt tcatttaatc agcatttcta cgaatgcgat
    11821 tataggtttt aaggatttgg ctcttatgct tagtgacata gcgataatct tctttaagag
    11881 tagcgatatc ctttaaagct ttcatgccat tacttgtttg ggcatcttta cgaataggtc
    11941 ggttactcgt tgactgccca aaggcatttt gaacatctaa agaaagcata aaattaataa
    12001 ataactttgc ttctttcata gaaggagcat tctttataat tgcaaccgaa gatgggacaa
    12061 aaactgtccc ttctgtcggg tatacaatag aaacattggc accacttttt tgcaaattga
    12121 cactagggtc ttcgtaagtc aaccccacaa tcatttttcc ttctgcaact gattgataaa
    12181 cttctgaaga gctagaagat ttgatagcat taatattatg ttgtagcttt ttaacatagt
    12241 tccacgcttt tggattggtg taaccaccct tggccaagag tatattagtg agttgtgaga
    12301 aagcactaga ggaagtattc ggatctgcaa aggcaatttt accttttaag gaaggctgta
    12361 ataaatcttc ataactcttg atggtaagtc ccttagctaa ttcgttattt acaatcaaga
    12421 cactcccatt tatagtataa ggtgtcgccg tatcactcgg atggatatag tctggaataa
    12481 cagtatgaac attctttgat acgtaagact caaacaatgc cttatgactt tcaaattgcg
    12541 tataatttcc tccaaagaaa atatccgcct tcaactgctt accctcctta cttaatctat
    12601 ctattagttg ccctgtccca ccttgaataa gcttaacttt tataccgtat ttttcctcaa
    12661 aagctggaat cgttcctgtt aaaatggctt gactatttgg acttagaata actaattctt
    12721 ttggtggaag tagtttagaa ggctggctaa tactgtatgt aaaaacactt ataaagataa
    12781 tgggaacaat cagtagtata taaataagcc ttttcgactg tttttctttc atctttcacc
    12841 tacctaatcc atctgtttaa aaatatgttc ttccttaaac gcttttactg tctgacccaa
    12901 gtacttttta aaaaccctat caaaataacg ataatcataa ataccaacag cttctgcaat
    12961 ttggtaaacc attaggtctg gttctctaaa catcaactga atggcttgat taatacgata
    13021 ttgatttata taatcactca aagttatatg aaggtgcttt ttagtgactg tgtaaagata
    13081 actttcactg taacctaaat cgtgagctaa ctggctaatc acaattttcc catgaaaatg
    13141 gctatgaatc catgctagca tatctttaat aacttcagga acttggtcat tagcttgtgg
    13201 tagaggtaaa tacaattctg tatgctcttg taggtgcttc tccttatgtt cctcaattct
    13261 agccataatt gtttttaaac aatcaatcaa agcttgcttt tctagtggtt ttgttaaaaa
    13321 ttcaagaacg ccatagtgaa tagctgattg cgcatttgga aaatcagcat agccagataa
    13381 aataattttg gcataagatt gtcctttggt tgcctcaaac atatcgaatg ctgtcataat
    13441 tggcatatta atatcactta aaacaatgtc tggctgctct tcctgaatta attgagctcc
    13501 ttctttacca tcttttgctt caccaaccac taaaatattc aatgattgat aatcaatagc
    13561 atagcgcaac cacttacgaa taaggtgctc atcctcaacg ataagtaatc tatacataac
    13621 tcactccttt actatatagc taactcgaaa agcatcatct gtcttcgtaa actctaacaa
    13681 tactgttgaa aattgatatt gtaatcgtct ataagaatta ataagcccat gatgcgagtg
    13741 atttgatctt aacattgtac gtatagcttt ttgcctagcc aaactaatac ccgatccatt
    13801 atttgaaaca gtaaaccaaa ttccatcact atcttgccaa atatctatgt ttattgctac
    13861 atcatgacgt tcttttaagc catatttaat agcattctca attagcggta acaagaaaag
    13921 ttttggtaca cgcatgtgtt caagttcagg agatactgaa atagtgtaag ttaattcttc
    13981 aaatctcact tgattaatca aaaggtagga ttcaatgatt gctaaatcat ccttaagcac
    14041 agctgcctct gtgctaccac taaggctata gcgcaataat tttgttagtt gtataacgat
    14101 tctttctgtt aattgtgaat catagtgact ggttattaaa attgtctcaa gggtattata
    14161 aaggaagtga ggattaaact gagcttctaa catcctcttt tcaaacagca acttttgtgt
    14221 ttccaaatcg agagttttct catgtaaatc ttttaaccgt gaaaccatct gattgatttg
    14281 aacagataga tattgaaatt catcttgact atcaagctca atactagatt tttcttggcg
    14341 ggaaatggca tccatatcta gcaccatttg atttatggcg ccagaatttt ttactgcgat
    14401 gcgatcggct agaactctag attttcgtcg taaaatcaca aaaataataa ctgatgatac
    14461 tagagaaaaa aggacgacca gagttactgg tatcagggga cgatacatat aaagtaggat
    14521 attatcttgc agagctactt tcctaacaac aaaagctcga tgatcgtgaa aactaaaata
    14581 tctagttaag aattgactat ctaccttatc taaactagag gagataaaat cacgattagt
    14641 aaaagtaaag ctattattta gctgatcggc aataatcaag tctgagttaa tagccttcgt
    14701 tggaagtaaa aagtctttcc cattcatcaa taaaaaggca taacctgaaa cttttttatt
    14761 aactataatt ggcttaatga gtgcaaggta atgccctccc tcactatcta caataatttt
    14821 tagtagcctt tgatttattt tagccttatc taaaacttca ctaatgtaaa tagacttact
    14881 aaaaaaatta cctaaatgtg aattactagc aaacaaaagt tcttgattag gtgagaatac
    14941 taaaagttct gtttgacttt gtaaatgatt ccttaaatga taatattgct tactcaaatc
    15001 agcacttcct aagtgtcctg agacaaatgg tttcaaacta tttttaccta agtcatctaa
    15061 taaggtatca tgtttttgaa tcaattgctt ataactatag gaaactgaat taacatcttt
    15121 ataaaggcct atctgctgag gcgctaaaga aaaaaggacg aaaagtgcaa tcaataacaa
    15181 ggataaaatt aaagactggc gggaaaaatg tttagagata tcatcttgta gtcgtttctt
    15241 aaagcgttct tccattctat atcctctcat ttccatctcc ttttattata gcaaatagct
    15301 atccaataaa gatagtcaac tcctttcata caataacttt caaaagaaaa aaagataagc
    15361 taaacaagta tcgtcttgtt ccacttacct ttaatgctag gagattatta gcaaatacct
    15421 aagagaccaa gtacaatacc taacaccata ataccaaaca tgatccagtt gatattgact
    15481 tttttacgta gaagccagaa ggctaatatc gttaccaaca gtggcactac gccaacaaat
    15541 aattggtcaa ggtaactttg tatggaagct gcttccttag ctcctttagc tgctaccgtt
    15601 aagattgtct tgaatttaac atttgaagca gtcattgagc ctaccatcat taaaccaagc
    15661 atacttgaag tcttagtaac aattttaata ccacctgatt catacaattt tttgataaac
    15721 cctgcaccta cagagtaacc tccatagaga ctataataat gaatcagaaa agctggaatg
    15781 ttatacaaga gtagaaagac aacagctccc atggctgagc ctgtacttgc taaggagata
    15841 ccgatacctg ccgcaataac ccttagaatt ccccaaaaga atgaatcacc aacaccagat
    15901 ataggtccca tcaaagaagc cttaactgcg gtgatagcac tagcgtcaaa atcaggatct
    15961 tcactgtttt tcttttccat agatgcaaca agacccataa taaaattatt aatgtgcatg
    16021 gtggcattaa accatgttgt atgacggacc agtgcctctg ctttatcctt atcagtctta
    16081 taaaaacgat tgatgactgg tagtaaagta taaataactc ctactgcgtg gtattgtgtt
    16141 gccccagtac gactggcatt catcgtccat gagcgccaga agactgagcg aagcatttta
    16201 cggtcttctt tagtcaaatt atcctgtgat ttcatgcgaa gaagtcctcc tcttctgatt
    16261 ctttagagtc aaaggttgtc tgtttactga tcgcacctct agtaattgcg tctaattcta
    16321 tatcacgttg tgaactaatg acgcaaataa ctgctcctag ggcagcaact gcaaccgctg
    16381 gtaattttaa ataagctgtt aaaacaaaac caagcaagta aaaaacagct aatttatttg
    16441 tccataataa tttcatcagc atggcaaaac caacagctgg aaggaggcct cccgccgcac
    16501 tcaagccatt catcaggttt tgaggaatat gatggacaaa actattaaca ggcccactac
    16561 ctactaaaat accaatgaaa gaaatagaag cgataatgag atagtaaata atccatgtac
    16621 catagtgaag catcacaagt ttaccttgat ggtttgcggc tgcggcttta tccaccatcg
    16681 gtgcaaagat attcatgaag acgtttttca aaaacataac aacaaatgct gccaacattc
    16741 caatcggaac agctagagtc attgctgctt tttgatcaat gtttgaaata atggtaaaag
    16801 ttgttgccat cgctgttgct gttactggct ctgcggcaat aacaccccca atattaacgt
    16861 ttcccaaaaa tagcgcttct agggaagcac ccattaagat accaacctta atatcaccta
    16921 gtagcattcc tgttacaaga ccaactacaa gtggtctctc catcatactt tgacctgtta
    16981 agtaatttcc cccaaaacaa atcaacacag ctaaagctgc catcgtcgct ggcattatca
    17041 tatgtcatac ctcactttgt ttgactagct gactcaagtt aaccttagta tttgaaggca
    17101 atacttggtt aaagacgtct aaatctggta agctggctaa ttcttttgca gcttccaact
    17161 cttctggatt taataagaga cttgatgtta gtttgacttt tgtagctgga tcagacttat
    17221 caaagcgtcc tacattagcg acgtttactg cttctaaatc ggttatattt ttagcaattg
    17281 tacaggcatc tttaacagaa ttgacaatca caaaaatgcg cttgtcagtt gcgcgtggat
    17341 ctttaaatag agctattgat tcttcaactg aacggattaa taatttcatg ccattaggca
    17401 cagccatttt aagagtcatc tgtgtgattt catttttggc agcctcatca ttagccacca
    17461 ctaaaagtgg tgcatttaat tctttagtcc atacaactgc cacttgcccg tgaatcaaac
    17521 ggtcgtcaac acgaatttga gtaatcattt ttttcctcct aattattaat cgaaaaagtc
    17581 atcttctgaa cacgctttaa agctgagatc taccagctgc acttgagatt cattgataac
    17641 ggttacaatt tcttctttag ttaattcctc ttcaggtttc aaaaatagta atgacaacaa
    17701 ggtcgctaaa ttgctgttcg ttattaaaaa aacattattt ttcccactat tcattacagc
    17761 agtcgccatt ttctgattaa cgctgccacc taacaaatct gtaaaaataa gcccctgttc
    17821 atctggtgca atctcatcaa taaattctac aatagacttg gtatagtcac tatcatcaat
    17881 gtaagcatct attgtctgaa tctcttgtcc cttgcctgtc agaatatcaa tagaactctg
    17941 caacccactc gcaagttttc cgtgactgcc gattaggaat ttccgcttca tctttctcct
    18001 cctatttatt acattaccct aaattagttc tgaaagtttt atcaatactt tgaataagat
    18061 aacctttact atcattctaa caaatgaaaa cggtttaaaa atggac
//
LOCUS       DATRQA010000030        17986 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.030, whole genome shotgun sequence.
ACCESSION   DATRQA010000030 DATRQA010000000
VERSION     DATRQA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17986
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.030"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..358)
                     /locus_tag="VBD27_000735"
     CDS             complement(<1..358)
                     /locus_tag="VBD27_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO7024765.1"
                     /translation="MTKQIIAIWAEDEDHLIGVNGGLPWRLPKELHHFKETTMGQALL
                     MGRKTFDGMNRRVLPGRETIILTKDEQFQADGVTVLNSVEQVIKWFQEHNKTLFIVGG
                     ASIYKAFLPYCEAIIKT"
     gene            complement(438..1277)
                     /locus_tag="VBD27_000736"
     CDS             complement(438..1277)
                     /locus_tag="VBD27_000736"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO7024766.1"
                     /translation="MTKADLLFKDNITKIMSEGVFSEQARPRYKNGEMANSKYITGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEIFWIYQDQTNDLAVLNDKYGVTYWNDWEVGHT
                     GTIGQRYGAVVKKHNIISKLLKQLEDNPWNRRNVISLWDYEAFEETEGLLPCAFQTMF
                     DVRRVNGELYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWRVGKFFYFINNL
                     HIYDNQFEQAQELLKRQPSECNPKLVLNVPDGTDFFDIKPDDFALVDYDPIKPQLRFD
                     LAI"
     gene            1326..2603
                     /locus_tag="VBD27_000737"
     CDS             1326..2603
                     /locus_tag="VBD27_000737"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEO7024767.1"
                     /translation="MRNKTLKVKAFFHIFSFFMVKWNLAKMTFIQKGLPMKIGIDKIG
                     FATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSITPVTEDIVTLAASAANDILSDED
                     KETIDMVIVATESSIDQSKAASVYVHQLLEIQPFARSFEMKEACYSATAALDYAKLHV
                     EKHPDSKVLVIASDIAKYGIKSTGESTQGAGSIAMLISQNPSILELKEDHLAQTRDIM
                     DFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKVYQKRFNTSLSDYAAFCFHIPFPKLAL
                     KGFNKILDNNLDEQKKAELQENFEHSITYSKKIGNCYTGSLYLGLLSLLENSQNLKAG
                     DQIAFFSYGSGAVAEIFTGQLVDGYQNKLQSDRMDQLNKRQKITVTEYEKLFFEKTIL
                     DENGNANFNTYRTGTFSLDSICEHQRIYKKINN"
     gene            2605..3888
                     /locus_tag="VBD27_000738"
     CDS             2605..3888
                     /locus_tag="VBD27_000738"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO7024768.1"
                     /translation="MTKISWTGFSKKSPEERIHYLEEQDFLADSSLEIVTNQDLLSLS
                     LANQMAENVIGRIALPFSLVPDVLVNGKVYQVPYVTEEPSVVAAASFAAKIIKRSGGF
                     LTTVHNRKMIGQVALYDVQDSQHTKESILNQKQQLLEIANAAHPSIVKRGGGACDLTI
                     EIKEDFLIVYLMVDTKEAMGANMVNTMMEALSSPLEDISKGKSLMSILSNYATESLVT
                     ATCRVDLRFLSRQKEEAIKLAQKMTMASQLAQVDPYRASTHNKGIFNGIDAIVLATGN
                     DWRAIEAGAHTYAVKDGQYRGLSRWSYKVDDNCLEGTLTLPMPVATKGGSIGINPSVH
                     LAHDLLGRPNAKELASIILSIGLAQNFAALKALVSTGIQAGHMKLQAKSLALLAGAKE
                     EQISEVVKQLLDSKHMNLETAQKIVNKLTKSKKRI"
     gene            3980..4429
                     /locus_tag="VBD27_000739"
     CDS             3980..4429
                     /locus_tag="VBD27_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEO7024769.1"
                     /translation="MNKNQYPKWEELPELDLYLDQVLLYVNQLINPKTITNDKLLTAS
                     MINNYVKHNYISKPIKKKYNRRQVARLIVITAFKQVFAIQEISQTLELLTADNHSEEA
                     YNGFAACMNKEEVHDLPPVVISACQTLNLYQETQKLVLELGGKNKNA"
     gene            4422..5078
                     /locus_tag="VBD27_000740"
     CDS             4422..5078
                     /locus_tag="VBD27_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEO7024770.1"
                     /translation="MLKEMSIKLSPQLSFGEELANSITHAVGALLMLILLPITAVYSH
                     NHFGLQAALGTSIFVTSLFLMFLSSSIYHSMTYNSLQKYVLRMIDHSMIYIAIAGSYT
                     PVALSLIGGWLGYLIIFLQWGITLFGILYKIFAPKINDKFSLVLYLIMGWLVIFIFPA
                     IITKTGPAFWGLLLAGGICYTIGALFYARKRPYDHMIWHLFILLASILQYIGIVYFML
                     "
     gene            5165..6079
                     /locus_tag="VBD27_000741"
     CDS             5165..6079
                     /locus_tag="VBD27_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO7024771.1"
                     /translation="MKSAYIFFNPKSGKDEQALAKEVKSYLIEHDFQDDYVRIITPSS
                     VEEAVALAKKASEDHIDLVIPLGGDGTINKICGGVYAGGAYPTIGLVPAGTVNNFSKA
                     LNIPQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEMKRKLGP
                     FAFLGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDG
                     LFRVYTMEHIHFFKLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRI
                     DGDPGDQLPVKVEVIPKALKFIIPNSLP"
     gene            6180..7034
                     /locus_tag="VBD27_000742"
     CDS             6180..7034
                     /locus_tag="VBD27_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO7024772.1"
                     /translation="MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLG
                     LDKHISISFVDDVPSDIGLIFSQPEQVTGAKSLRDIYHLTDPTYQGPYTIPILIDKTD
                     NRIVCKESADLLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINKNFQSLMYKLA
                     FLDKQADYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDL
                     LLLNQKALEDYPNIFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTG
                     PNMAKWEK"
     gene            complement(7080..7298)
                     /locus_tag="VBD27_000743"
     CDS             complement(7080..7298)
                     /locus_tag="VBD27_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEO7024773.1"
                     /translation="MIKLYYKDAYDTERCQVFENPNDFIRVLMGCVTVPDNYPITKMT
                     YKDKDIAYSGTYGDLFKTFIEFDWSTLD"
     gene            complement(7366..8361)
                     /locus_tag="VBD27_000744"
     CDS             complement(7366..8361)
                     /locus_tag="VBD27_000744"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO7024774.1"
                     /translation="MTNRKDDHIKYALKYQSHYNSFDDIELIHSSLPKYNVNDIDLST
                     HFAGQSFEFPFYINAMTGGSEKGKAVNHKLAQVAQATGIVMVTGSYSAALKNDEDDSY
                     PTTDLYPDLKLATNIGLDKPVPAAESTVKAMNPIFLQVHVNVMQELLMPEGEREFHMW
                     RSHLKEYVDNIQCPLILKEVGFGMDLQSIKDAYDIGITTVDISGRGGTSFAYIENQRG
                     RDRSYLNTWGQTTAQSLINAQSMMDKMDILASGGIRHPLDMVKCLVLGAKAVGLSRTV
                     LELVERYPVDDVIAILNSWKEDLRMIMCALNCKKITDLRQVNYILYGQLKEANAK"
     gene            complement(8358..9350)
                     /locus_tag="VBD27_000745"
     CDS             complement(8358..9350)
                     /locus_tag="VBD27_000745"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEO7024775.1"
                     /translation="MVKVQTGGKLYIAGEYAILYPGQVAILKNVPIYMTALATFADNY
                     SLYSDMFNYTASLQPDKQYSLIQETILLMEEWLINFGKNIKPIHLEITGKLERYGLKF
                     GIGSSGSVVVLTIKAMAALYEIEMPSDLLFKLSAYVLLKRGDNGSMGDIACIAYEHLI
                     SYSAFDRRAVSKMIETKPLEQVLEAEWGYRITKIQALLEMDFLVGWTMQPSISKEMIN
                     IVKSTITQRFLDDTKYQVVQLLSAFKEGDKEAIKRCLEEISLLLFNLHPSIYTDKLQK
                     LKEASKGLDIVTKSSGSGGGDCGIAISFNKNDNQTLIKRWESAGIELLSKETLS"
     gene            complement(9343..10287)
                     /gene="mvaD"
                     /locus_tag="VBD27_000746"
     CDS             complement(9343..10287)
                     /gene="mvaD"
                     /locus_tag="VBD27_000746"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO7024776.1"
                     /translation="MDGKSISVKSYANIAIIKYWGKADAEKMIPATSSISLTLENMYT
                     ETRLTALGKDAKKDEFYISGVLQNDHEHDKMSAILDRFRQNRSGFVKIETTNNMPTAA
                     GLSSSSSGLSALVKACNDFFGTNLSQSQLAQEAKFASGSSSRSFFGPVAAWDKDSGDI
                     YKVHTNLDLAMIMLVLNDKRKPISSREGMKICTETSTTFNEWVRQSEQDYQDMLVYLK
                     NNDFQKVGQLTERNALAMHSTTKTATPAFSYLTEETYKAMDVVKKLREKGHECYYTMD
                     AGPNVKVLCLRQDLEALAAILEKDYRIIVSTTKELADG"
     gene            complement(10269..11147)
                     /gene="mvk"
                     /locus_tag="VBD27_000747"
     CDS             complement(10269..11147)
                     /gene="mvk"
                     /locus_tag="VBD27_000747"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO7024777.1"
                     /translation="MKEKFGIGKAHSKIILMGEHSVVYGYPAIAIPLKNIEVTCLIEE
                     APQLIALDMTDPLSTAIFAALDYLGKTSSKIAYHIESQVPERRGMGSSAAVAIAAIRA
                     VFDYFDEDLEADLLECLVNRAEMIAHSNPSGLDAKTCLSENTIKFIRNIGFSTVPMHL
                     NAYLVIADTGIHGHTKEAVDKVKSSGEAVLPFLKELGYLAEASEDAIHKSDSKQLGSL
                     MTKAHQSLKQLGVSSLEADHLVEVAISCGALGAKMSGGGLGGCIIALVKEKREAERLS
                     QQLEREGAVNTWTEKV"
     gene            complement(11263..12492)
                     /locus_tag="VBD27_000748"
     CDS             complement(11263..12492)
                     /locus_tag="VBD27_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7024778.1"
                     /translation="MFRNLRLRFIGIAALAILVVLFSVVGVLNSANHYQTKNEIYRVL
                     TILADNNGRIPNKLEFSKELGDDLSTDAIFQFRYFSARTDAKGNVTSFDSRNIFEVSD
                     RQIKNYAKRIVSQNSHSGHITYNFSTYSYLLKKVGKNDYLVVFLDTTNQYLDNQRLLQ
                     LSIWMSLVSFIVFMVIVSVLSGRVILPFVANYEKQRRFITNAGHELKTPLAIISANNE
                     LVEMMSGESEWTKSTNDQIQRLTGLINGMVSLARFEEQPDISMVDLDFSHITKDAAED
                     FKGPIIKDGKDFIMSIQPGIHVKAEEKSLFELVTLLVDNANKYCDPMGTVTVKLSRSS
                     RLRRAKLEVSNTYKNGKDIDYSKFFERFYREDESHNNKKSGYGIGLSIVTSLVHLFKG
                     SIDVNYKHDTITFVIYI"
     gene            complement(12492..13178)
                     /locus_tag="VBD27_000749"
     CDS             complement(12492..13178)
                     /locus_tag="VBD27_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7024779.1"
                     /translation="MRVLIAEDEEQMSRVLSTAISHQGYVVDVAYDGQTAIDLANQNA
                     YDVMVMDVMMPVKTGVEAVKEIRQSGNKSHIIMLTAMAEIDDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMSRRLEDFTPNVLSLGRVTLSVGEQELQCENTIRLAGKEAKMLAFF
                     MLNHDKELSTQQLFEHVWGADKDQEDVDEGYVFIYVSYLRQKLQAIGANLEIIGQEGS
                     SYKLSEINGG"
     gene            complement(13180..13806)
                     /locus_tag="VBD27_000750"
     CDS             complement(13180..13806)
                     /locus_tag="VBD27_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO7024780.1"
                     /translation="METNIYGDYGRYLPLILEDFSQRIQLENDKAKVETGYKLYEHII
                     GRIKTSDSMIEKCRRKQLPVTVDSALKVIRDSIGVRIICGFVNDIYQIVERIKTFDDC
                     RIVVEKDYIQHVKPNGYRSYHVILEIDTPYPDCLGNSEGKYYMEIQLRTIAQDSWASL
                     EHQMKYKHDIENPERIVRELKRCADEIASVDLTMQTIRQLIESGTKKE"
     gene            complement(13914..15668)
                     /locus_tag="VBD27_000751"
     CDS             complement(13914..15668)
                     /locus_tag="VBD27_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7024781.1"
                     /translation="MKTKSQKTSFFSNLSPYLKGYKLLFALAIVLATLSSLTTVIGPD
                     RLKEMTDLMVKGLAGNIDLAKIGHIAGQLAVLYVLGALVSYLASFIVTTIIQKFAQGM
                     RNAIADKINKIPLSYFDSHTQGDTLSRVTNDVDLMTQSFNQSLVTMVASTVLLVGSLI
                     MMFKTDWHLATTAIISVFSGFALSSIIMVKSQPLFKKQQVNLANISGYVEEIYSGHNI
                     VTSHNARQQAKSDFQLLNDKLHSSMWKSQFFSGIMMPLMQFIGNFGYVMVCVVGAMLA
                     IKGTITMGTIVAFMTYVRIFTQPIGQIAQGITQLQSASAAMGRVFEFLNETNMQEDKH
                     KVLNLPKFKGHVSFEHVKFGYSDNKTIIHDFTSEAQPGQKVAIVGPTGAGKTTIVNLL
                     MRFYDVNSGSISIDDINIQDMKRSQVHDAFSMVLQDTWLFEGTIKENLIFNQTTISDD
                     EVQEAAKAVGIHHFIMTLPKGYDTVLDDAVTLSVGQKQLLTIARALLKNSPLLILDEA
                     TSSVDTRTEELIQKAMDKLMEGRTSFVIAHRLSTIKNADLILVMKDGNIIEQGNHDVL
                     MSQSGFYADLYNSQFESA"
     gene            complement(15658..17475)
                     /locus_tag="VBD27_000752"
     CDS             complement(15658..17475)
                     /locus_tag="VBD27_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7024782.1"
                     /translation="MFNIFKRLNGKEWLMIVASTILVCLAVWMDLKTPEYMSDITRML
                     QTKGTKLEALWDPGLKMLAFSFGSFLVAVMVGFLGARTAASFTSRLRSDIFNHVMDYS
                     DAEIKRFSIPSLLTRTTNDLTQLQILIVMGMQVVTRGPIMAIWALTKIGGKSDQWTGA
                     VGVAVIIVMILLSVLLLLAFPRQRQIQGFTDALNATTREALSGVRVIRAYNAEKYQTD
                     KFKRENDDLTKLNLFVYRLMSLMNPVMTVVSSGLTLAIYWIGSYLINDISVPKVTGTS
                     VSVLAAKSAITDRVDLFSNMVVFSSYAMQVVMGFMMMVAIFIIMPRALVSARRINEVL
                     TLAPTVTFKENSHIQSAPKGQIEFQDVSFHYSKDSEAVIEHVSFKADTGQTVAFIGST
                     GSGKSTLVNLVPRFYDATEGSIKLDGIDIKEYSHNDLNNKIGYIPQKAVLFSGTVRSN
                     MIFGESGREELSDEQIWQALELAQAKEFIEAKEKGLDTEVAQGGSNFSGGQKQRLAIA
                     RALARHPEILIFDDSFSALDYKTDHILRHVLKEKTAAMTKLIVAQRISTIMDADLILV
                     LDQGKVVGQGTHKELLSNNEVYQEIAYSQLSKEELENEN"
     gene            complement(17522..17962)
                     /locus_tag="VBD27_000753"
     CDS             complement(17522..17962)
                     /locus_tag="VBD27_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7024783.1"
                     /translation="MENPLQKARILVNQLEKYLDRYAKEYDVEHLAGPQGHLVMHLYK
                     HPDKDMSIKDAEEILHISKSVASNLVKRMEKNGFIAIVPSKTDKRVKYLYLTHLGKQK
                     ATQFEIFLEKLHSTMLAGITKEEIRTTKKVIRTLAKNMAMEDFD"
BASE COUNT         5765 a         3533 c         2845 g         5843 t
ORIGIN      
        1 tagtttttat gattgcttca caataaggca gaaatgcttt ataaatactt gcaccaccta
       61 caataaataa ggtcttatta tgttcctgaa accattttat aacttgttca acactattta
      121 ggactgtcac tccatctgct tggaattgtt catcttttgt taagatgatt gtctctctac
      181 caggtaaaac acgacggttc attccatcaa aggtctttcg tcccataagc aaagcctgcc
      241 ccatggtcgt ttctttgaag tgatgtaact ctttaggaag cctccatggt aaaccgccat
      301 taactccaat caaatggtct tcatcttcag cccaaatggc aattatttgc tttgtcatga
      361 cttttttcct cactaactta tctctagata ttctaacaaa ttttacctca aattgcttgt
      421 taaaaaacat gagatagtta gattgctaaa tcaaaacgta attgaggttt aattgggtca
      481 taatcaacta acgcaaaatc atctggttta atatcaaaaa agtctgttcc atctggaaca
      541 ttcaatacta gtttggggtt acactcagac ggttggcgtt ttaagagttc ctgagcttgt
      601 tcaaactggt tatcataaat atgaagatta ttgataaaat agaaaaactt cccaactctc
      661 caaccaaaat gctttgcaat catcatctga agtgctacat actgcatagc attaatatga
      721 tgtgcaacta acatgtcatt agaacgttgt gttaatgtgg catccaaata aagctcacca
      781 ttaacgcgtc tcacatcaaa catcgtttga aaagcacaag gtagtaggcc ttctgtttct
      841 tcaaatgctt catagtccca taatgaaata acattacgac gattccaagg gttatcttca
      901 agctgtttta agagttttga tataatatta tgttttttaa caactgcacc gtagcgctga
      961 cctatcgtgc cagtatggcc aacttcccaa tcattccagt atgtcacacc atatttatca
     1021 ttcaaaactg ctaaatcatt tgtctgatct tgataaatcc agaaaatttc tttaatagca
     1081 gattttattg gaattggacg tagagttgta ataggaaatt caccctttga taaatcatac
     1141 tctgcaaagg cacctgtaat atacttagaa ttagccattt ccccattttt atatctaggt
     1201 ctggcttgct cgctaaatac accttcagac ataattttcg tgatattgtc tttaaataat
     1261 aaatcggctt ttgtcatgag atacctccaa agtcttattt tatcagattt taaaaagata
     1321 ttctagtgag aaacaagact ttgaaggtta aagctttttt tcatattttt agcttcttta
     1381 tggtaaaatg gaatttagca aaaatgactt ttattcagaa aggtttacca atgaaaatag
     1441 gaattgataa gattggtttt gcaaccagtc aatatgtcct tgaaatgact gatttggcta
     1501 ttgctcgcca agttgaccct gaaaaattca gcaagggatt attgttagat tcacttagta
     1561 ttacgccagt tacagaagat atcgttacac ttgcagcttc tgcagctaat gatattttat
     1621 ctgatgagga caaagaaact attgatatgg tgattgtcgc tacagaatca agtatcgatc
     1681 agagtaaggc ggcttctgtc tatgtacatc aacttttaga gattcaacct ttcgcaaggt
     1741 cattcgagat gaaagaagca tgctatagcg caactgctgc tcttgattac gctaaattac
     1801 atgtagaaaa acatccagat tctaaagtat tagtgatagc ttcagatatt gctaaatatg
     1861 gcattaaatc tactggagaa tccactcaag gtgcaggcag tatcgctatg cttattagtc
     1921 aaaatccatc cattctagag ctaaaagagg accatctagc gcaaacccgt gatattatgg
     1981 acttttggcg accaaattat tctgatgttc cttatgttaa tggcatgttt tcaacaaaac
     2041 aatatctaga tatgctaaaa actacttgga aagtatatca aaaacgtttt aatactagtc
     2101 taagtgacta tgctgctttt tgtttccata ttccttttcc aaaattagct ttaaagggct
     2161 ttaataaaat tctagataat aacttagatg aacaaaagaa agctgaacta caagaaaatt
     2221 ttgagcactc tattacctat agtaagaaaa ttggaaattg ctatactggc tcattgtatc
     2281 taggacttct atcgttacta gaaaatagtc aaaatttaaa agcaggtgat caaattgcct
     2341 tcttttcata cggtagcgga gctgttgctg aaatttttac cggtcagtta gtagacggct
     2401 accaaaataa acttcagagc gatcgtatgg atcaactcaa taaacgtcaa aaaattacag
     2461 taactgagta tgagaaactc ttctttgaaa aaacaatcct agacgaaaat ggaaatgcta
     2521 acttcaacac ctaccggact ggcacttttt ctctagactc tatttgcgaa catcaacgta
     2581 tctacaagaa aattaataat taatatgaca aaaatttcct ggacaggatt ttcaaaaaaa
     2641 tcccctgaag aacgtattca ttaccttgaa gaacaagatt ttttagctga ttcttcccta
     2701 gaaatagtga cgaatcagga cttactctct ttatcactag ccaatcaaat ggctgaaaat
     2761 gtcatcggtc gaatagcctt accattttca cttgtcccag atgttttggt taacggtaaa
     2821 gtctatcaag tcccatatgt cactgaagaa ccgtcagttg ttgctgctgc aagttttgct
     2881 gctaaaatta taaaacgatc tggtggtttt ctgacaaccg tccataatcg taaaatgatt
     2941 ggacaagtag ccctttatga tgtacaagat agtcaacaca ctaaagaatc tatattaaat
     3001 caaaaacagc aattattaga aatagccaac gctgctcatc catcaatagt caagcgtgga
     3061 ggcggtgctt gcgacctaac tattgagatt aaagaagact ttttgattgt ttaccttatg
     3121 gttgatacaa aagaagccat gggtgctaat atggttaaca ccatgatgga agcattatct
     3181 tcgccattag aagacatttc aaaaggcaaa agcttaatgt caattttatc taactatgct
     3241 accgaatctt tagtgactgc tacatgccga gttgatttaa gatttctaag tcgccaaaaa
     3301 gaggaggcca tcaagttagc tcaaaaaatg acgatggcaa gtcaattagc tcaagtagac
     3361 ccttatcgtg ctagcacaca taataaaggt atttttaatg gcattgatgc tatcgtctta
     3421 gctactggaa atgattggcg agctattgaa gctggagcgc atacctatgc tgtcaaagat
     3481 gggcaatatc gaggattatc tagatggtct tataaggttg atgataattg cctagaaggt
     3541 actctgacgc taccgatgcc tgttgcaaca aaaggtggat ccattggtat taaccctagt
     3601 gtccacctag ctcacgatct actaggtcgt cctaatgcta aagagttggc aagtattatt
     3661 ctttcgattg gactagctca aaattttgcg gctcttaagg cattggttag cacaggaatt
     3721 caagctggtc acatgaaact tcaagcaaag tcgttggcac tacttgctgg agctaaagaa
     3781 gaacaaattt cagaagtcgt taaacagctt ctagactcta aacacatgaa tctagagaca
     3841 gcacaaaaaa ttgtcaataa acttactaaa agtaaaaaaa gaatttgaga aaaatcatct
     3901 caaattcttt ttttacttga cttgaatttc cataagtgta taatctagtt ataaagacta
     3961 gattatatgg agttttaaaa tgaataaaaa tcaataccca aaatgggaag aattacctga
     4021 actagatttg tatcttgacc aagtacttct ctatgtgaat caattaatta atcctaaaac
     4081 aataactaac gataaactac tcaccgcttc gatgatcaac aattatgtca aacataatta
     4141 tatatccaaa cctataaaaa aaaaatacaa tcgccgtcaa gtagcacgat tgattgttat
     4201 tacagctttt aaacaagttt ttgctattca agaaatcagt caaacattag aacttctgac
     4261 agctgacaat cattctgaag aagcatataa cggatttgct gcttgtatga acaaagaaga
     4321 ggttcacgat ttaccaccag ttgtcattag tgcctgtcag acgttgaacc tttatcaaga
     4381 aacacaaaaa ttagttttag aactaggagg aaaaaataaa aatgcttaaa gaaatgtcta
     4441 tcaaattaag tcctcaactt tcctttggcg aagagcttgc aaatagtatc actcacgctg
     4501 taggagcttt gcttatgcta atcctattac caatcaccgc tgtctatagt cacaaccact
     4561 tcggattaca agcagcactt ggtacctcaa tattcgtcac ttctttattt ttgatgtttt
     4621 tatcatcaag tatttaccac tccatgactt ataacagtct tcaaaaatat gttctacgca
     4681 tgattgatca ctctatgatt tatattgcta ttgcaggtag ttatactcct gtcgcattgt
     4741 cacttattgg cggctggtta ggatatctca tcattttttt acaatggggg attacattat
     4801 ttggtatcct ttataaaatc ttcgctccca aaatcaatga taaatttagc cttgtacttt
     4861 acctcattat gggatggctg gttatcttta ttttccctgc tattatcaca aaaactggac
     4921 cagctttttg gggattactc ttggctggtg gtatttgcta tactatcggt gcactctttt
     4981 atgctcgcaa acgtccttat gatcacatga tttggcacct ttttattcta ttagcttcta
     5041 ttttacaata cattggaatt gtctacttca tgctataaaa tttaactaca ttttattgat
     5101 tatttttttg ctaaaccatg atataataat aatcatctaa atcttagaga gaaggcgtgg
     5161 acatatgaaa agtgcttata tcttttttaa tccaaaatct ggtaaagatg aacaagcatt
     5221 ggcaaaagaa gtaaaatctt atttaataga acatgatttt caagacgatt atgttcgtat
     5281 tattacacct tcatctgttg aggaagctgt cgccttagca aaaaaagcat ctgaagacca
     5341 tatcgacttg gttattcctt tagggggaga tggcactatt aataaaatct gtggtggtgt
     5401 ctacgctggt ggtgcttatc ctactatcgg actagtacct gccgggaccg tcaataactt
     5461 ttcaaaagct ttgaacatcc cgcaagagag gaatctcgct cttgaaaacc ttctaaacgg
     5521 tcatgtaaag tccgtagata tctgtaaagt taatgacgat tacatgatta gcagtttaac
     5581 acttggtctc cttgctgata ttgctgctaa tgtcacttct gaaatgaagc gtaaattagg
     5641 cccttttgct ttcttaggtg atgcctaccg tatattaaag cgtaatcgat catattctat
     5701 cactcttgct tacgataata atgtacggtc tttacgcacg cggttactcc ttattaccat
     5761 gaccaattcg attgccggta tgccagcctt ctctccagaa gcaacaattg atgatggact
     5821 ttttagagtc tacactatgg aacatattca cttcttcaaa ttattgttgc atctaagaca
     5881 atttcgtaag ggagatttta gtcaagcaaa ggagataaaa cattttcata ccaataatct
     5941 gactatttca acttttaaac gtaaaaaatc agctatccct aaagttcgta ttgatggcga
     6001 tccaggtgac caattacctg taaaagttga agtcatccct aaagccttaa agtttatcat
     6061 tcctaactca ttaccataat ataaaaggac ctagacataa gtattagagt aaatactatc
     6121 tgtctaggtt cttttttgtt atacttaaag cgataaatat cattgaaagg aagtatacga
     6181 tgacaacatt tactgctaaa tttattgacc aagagtggga agtcccagta gaatcaggtc
     6241 gctaccatat gattgttggg gaattttgtc cttatgcaca acgtccgcag attgcgcgcc
     6301 aactgcttgg tttggataaa catatttcta tcagttttgt ggatgatgtt ccaagtgata
     6361 taggacttat ttttagccaa ccagaacagg tcactggtgc taagtcgctt cgtgatattt
     6421 atcatttgac tgacccgacc taccaaggtc cttatactat cccaatttta attgacaaga
     6481 ccgataatcg tattgtctgt aaggaatctg cagatttgct tcgacttttt actacagatt
     6541 tctctgattt gcatcaagag gatgctcctg tccttttcag ccaagaaact gcttcactta
     6601 ttgataatga cattaaagac attaataaga acttccaaag cttaatgtat aagttagctt
     6661 ttcttgataa acaagcagat tatgatactt atagtaagga attcttcact tttctagatc
     6721 aaaaagaaca cttactagga caacgtcctt tcttgctcgg agataatcta agtgaggttg
     6781 atattcattt ctttacacca ttagtccgat gggatattgc tgggcgtgat cttctactac
     6841 ttaatcagaa agcgctagaa gactacccaa atattttttc ttgggctaaa actttataca
     6901 atgactttaa tttaaaaaca ttaaccaatc cacaatccat aaaaaataac tattacttag
     6961 gcaaatttgg aagagctgtt cgtcatcata ccatcgtccc aacaggtcca aatatggcaa
     7021 aatgggaaaa ataaagctaa aaagctaagg caattcacag ccttagcttt ttagctatac
     7081 tagtccaaag tggaccaatc aaattcaata aaagtcttga agagatctcc ataagtacca
     7141 ctgtaagcaa tatctttatc tttataagtc attttcgtaa taggataatt gtcaggaaca
     7201 gtaacacatc ccattaaaac tcgaataaaa tcatttggat tttcaaaaac ttggcatcgt
     7261 tcagtgtcgt aagcatcttt ataatacagt ttaatcatct tattacctct gacgttttac
     7321 aaggaaactt tgacaggaag ctatatctac ttcacaaaaa taatatcatt tagcattggc
     7381 ttccttcaat tgaccataca atatataatt gacttgtcgt aaatcagtta tttttttaca
     7441 attcagtgca cacattatca ttcttaaatc ttctttccaa gaattgagaa ttgcaataac
     7501 atcatctaca ggataacgct caactaattc taaaactgtt ctagaaagtc caacagcttt
     7561 agcacccaac actaaacatt ttaccatatc taaaggatga cgaatgccac cagatgctag
     7621 gatatccatt ttgtccatca tagattgagc gtttattaag gattgagctg ttgtttgccc
     7681 ccaggtattg agatacgaac ggtcgcgtcc acgttgattt tcgatgtaag caaaactagt
     7741 ccccccacgc cccgaaatat ctacagttgt gatacctata tcgtaagcat cctttattga
     7801 ttgtaaatcc atcccaaaac caacctcttt taaaataagt gggcattgga tattgtcgac
     7861 gtattctttt aagtgagaac gccacatgtg aaattcacgc tctccttcag gcatcaaaag
     7921 ttcttgcatc acattaacat gaacttgtaa aaaaatgggg ttcatagcct taacggttga
     7981 ctctgctgct ggtacaggct tgtctaaccc tatatttgtc gccagtttta aatcaggata
     8041 caagtctgtt gttgggtagg aatcatcttc atcgtttttg agagctgccg aatatgaacc
     8101 tgtcaccata acaatccctg tagcttgagc aacctgtgcc aatttatgat tgactgcttt
     8161 ccctttttca ctaccgcctg tcatagcatt gatataaaaa ggaaattcaa agctttgacc
     8221 agcaaaatgt gttgataaat cgatatcgtt gacattatat tttggtaagg atgaatgtat
     8281 taactcaata tcgtcaaaac tattataatg cgactgatat ttaagagcat attttatgtg
     8341 atcatctttt cgatttgtca tgacagtgtc tccttactaa gtagttcaat accagcactc
     8401 tcccaacgct tgattagagt ttgattatca tttttattaa aggatatggc tataccacaa
     8461 tcgcctcctc cagatcctga agattttgtg actatatcta ggcctttgct tgcctcttta
     8521 agcttttgaa gtttatcggt gtaaatagat ggatgaagat taaataatag caagctaatt
     8581 tcttctaaac atctcttaat cgcttcttta tctccttctt taaaggctga aagtaactgg
     8641 acaacttgat actttgtatc atctaaaaaa cgctgcgtaa tagttgattt aactatattt
     8701 atcatttctt ttgaaatgga cggttgcatt gtccatccca ctaaaaaatc catctccaat
     8761 aaagcttgta ttttggtaat acgataaccc cattcagctt ccaatacttg ttccaaaggt
     8821 tttgtttcaa tcatttttga aacagcccta cgatcaaaag ctgaataaga aattaaatgc
     8881 tcataagcaa tacaagcaat atctcccatt gagccattat cacctctttt taagagaaca
     8941 taggcagata atttaaaaag caagtcgctt ggcatctcta tctcataaag cgcagccata
     9001 gctttaatag tcaaaacgac aacacttccg ctagagccta taccaaattt aagtccatag
     9061 cgctctaatt ttcccgtaat ttcgaggtga atcggtttta tatttttgcc aaaattaatc
     9121 aaccattctt ccataagcaa tatcgtttcc tgaatgagac tatattgctt atctggctgt
     9181 aagcttgccg tatagttaaa catatcagaa taaaggctgt agttatctgc aaaagtagct
     9241 aatgcagtca tataaatagg aacgttcttt aaaatagcta cttgcccagg atacagtatc
     9301 gcgtattcac ctgcaatata taatttgcca cctgtttgca ccttaaccat ctgctaattc
     9361 ctttgtcgtt gaaacaataa tacgataatc tttctccaag atagcagcta atgcctcaag
     9421 gtcttgacgt aagcaaagta ctttaacatt tggacccgca tccatcgtat aataacattc
     9481 atgacctttt tcgcgaagtt tcttcacaac atccatagct ttatatgtct cttctgtcaa
     9541 atatgaaaag gctggagttg ctgttttagt tgttgagtgc attgcaagtg cattacgctc
     9601 tgtcagttga cctacctttt gaaaatcatt attttttagg tatactaaca tatcttgata
     9661 atcttgttca gattgcctta cccattcatt aaaagtagtt gaagtttcgg tgcagatttt
     9721 cataccttca cggcttgaaa tcggctttct tttgtcattt aagaccagca taatcatagc
     9781 taaatctaaa ttagtgtgaa ctttatagat atcaccacta tctttatccc aagcagcaac
     9841 aggtccaaaa aaagagcgtg aagaggagcc tgaagcaaac ttggcttctt gagctagctg
     9901 tgattggcta aggttagtac caaaaaaatc attgcaggcc tttactaagg ctgataaacc
     9961 acttgatgac gatgataagc ctgcagctgt tggcatatta ttagttgttt caatctttac
    10021 aaagccggac ctattttgtc gaaagcgatc caagatagct gacattttgt catgttcatg
    10081 atcattttga agaacaccag agatgtaaaa ttcatctttc ttagcatcct tcccaagggc
    10141 agtcaaacgt gtttcagtgt acatgttttc aagtgttaga gaaatactac tggtagctgg
    10201 aatcattttt tcagcatctg cttttcccca atatttgata atagcaatgt tagcataaga
    10261 cttaacactt atactttttc cgtccatgtg ttgacagctc cttctctttc taattgctga
    10321 cttaagcgct cagcttctct tttttctttt acaagtgcga taatacaacc acctaatccg
    10381 ccgccactca ttttagcgcc tagagcacca caactaatag ctacctctac cagatgatca
    10441 gcttctaaac ttgagacacc caattgtttt aaactttgat gtgctttagt catcaagcta
    10501 ccaagttgtt tggaatcact tttatgaata gcatcctctg atgcctctgc taaataacct
    10561 aattctttta aaaagggtag tactgcctcg ccagatgatt tcaccttatc cactgcttct
    10621 ttagtatgcc catgaattcc tgtatcagca ataactaaat aagcattcaa atgcatgggt
    10681 actgttgaaa aaccaatatt gcggataaat ttaatagtat tttcactaag acaagttttt
    10741 gcatctaatc cactcggatt tgaatgtgct atcatttctg ctcgattaac aaggcattct
    10801 aacaaatctg cctccaaatc ttcatcaaaa taatcaaaaa ctgcacgaat agctgcaata
    10861 gcaactgcag ctgatgatcc cataccacgt ctttctggga cttgtgactc aatatgatat
    10921 gcaattttag aagaagtttt acctaaatag tccaaggcag caaaaatggc tgtcgacaaa
    10981 ggatcagtca tatctaatgc tattaattga ggagcctctt caataaggca agttacttca
    11041 atatttttta aaggaatagc aatggctgga taaccatata ctactgagtg ctcacccatc
    11101 aagataatct tgctatgagc ctttcctata ccaaactttt ctttcatctt tttctcttta
    11161 caattctatt accttactat tttaatataa atccttaaaa aaagcgactt taacgtgata
    11221 ataacataaa ttaaaggaac tacaattgca gtccctttaa tcttaaatgt aaatgacaaa
    11281 ggtaatggta tcatgtttat aattcacatc gatacttcct ttaaatagat gcaccaaact
    11341 agttacgata gaaagaccaa taccatatcc cgattttttg ttattatgtg actcatcctc
    11401 tcgataaaat cgttcaaaaa acttgctata gtctatgtct ttaccattct tataagtatt
    11461 tgaaacttca agcttagctc gtctcaaacg acttgaacgt gataacttaa ctgtcacagt
    11521 ccccatagga tcacaatact tattagcatt atcaaccaat aatgtaacca attcaaataa
    11581 tgatttttct tcagctttga catgaatacc tggttggata ctcattataa agtctttacc
    11641 atctttaatg attggacctt taaaatcttc tgcagcatct tttgtaatat gtgaaaagtc
    11701 taagtcaacc atacttatat caggctgttc ttcaaagcgg gcaagactga ccatgccatt
    11761 aattagaccc gttaaacgtt ggatttgatc atttgtagat ttagtccact ctgattcacc
    11821 actcatcatt tccaccaatt cattattagc ggagatgata gcaagtggag tttttagttc
    11881 atgtcctgca ttggtaataa aacgcctttg cttttcatag ttagcaacaa atggtaagat
    11941 aactctccct gataaaactg aaacaattac catgaagacg ataaaactta ccaaggacat
    12001 ccatattgaa agttgcaata atctttgatt atcaagatat tggttggttg tatctaaaaa
    12061 taccactaaa taatcatttt taccaacctt ttttagtaga taagaataag tactaaaatt
    12121 ataggtgata tgacctgaat gactattttg tgatacgata cgcttggcat aattttttat
    12181 ttgcctatcg cttacttcaa agatatttct agaatcaaaa ctagtcacgt taccttttgc
    12241 atctgttcgc gcactgaaat aacggaattg gaaaatagcg tccgttgata ggtcatcacc
    12301 taattcttta ctaaattcaa gtttattcgg tatacgtcca ttgttgtctg caaggatagt
    12361 taacacacga taaatttcat tcttcgtttg atagtgatta gcagagttga ggacaccgac
    12421 tactgaaaat aagacaacaa gaatagctag cgcagctatt ccgataaaac gaagtcgtaa
    12481 atttctaaac attagccacc atttatttca cttaatttat agctactacc ttcttgacca
    12541 ataatctcca agttagcacc aattgcctgc aatttttgtc tcaaataaga aacatagata
    12601 aagacgtatc cctcatctac atcttcttgg tccttatctg ctccccagac atgttcgaaa
    12661 agttgttgtg tagataattc tttatcatgg ttcaacatga aaaatgctaa cattttagct
    12721 tctttacccg ctagacgaat agtgttttca cattgcaact cttgttcacc gactgataag
    12781 gttactcttc ccaatgacaa tacattaggt gtgaaatctt ccaatcttcg ggacatagag
    12841 cgaagccttg ctaataattc ttttagagag aagggttttg ttaaataatc atctgcacca
    12901 gcatctagac ctgttactct atcatcaatt tctgccatgg ctgttagcat aataatgtga
    12961 gatttatttc ctgattgtct aatttctttg acagcctcaa cacctgtttt aacaggcatc
    13021 atgacatcca taaccatgac atcatatgca ttttgattag ctaaatcaat agcagtctga
    13081 ccatcgtagg cgacatcaac aacataacct tgatgactaa ttgccgtact aaggacacgt
    13141 gacatctgtt cttcatcttc tgcaattaat actctcattt tattccttct ttgttccact
    13201 ctctatcaat tgacgaatcg tttgcatcgt taaatcaaca gatgctattt catcagcaca
    13261 acgttttaat tctcttacaa ttcgttcggg attttcaata tcatgcttgt atttcatctg
    13321 atgttctaaa ctagcccaag aatcttgcgc aattgtacgc aactgaatct ccatgtaata
    13381 tttaccttct gaattaccca aacagtccgg atagggagta tcaatttcta aaatcacatg
    13441 gtaagagcga tacccatttg gcttaacatg ctggatatag tctttttcaa ctacaatacg
    13501 acaatcatca aatgtcttaa tacgttctac aatttgataa atgtcgttaa caaaaccaca
    13561 aataatacgg actccaatac tatctctaat aacttttagt gcagaatcta ctgttacagg
    13621 taactgttta cgacgacatt tctctatcat actatctgat gtcttaatac gaccaatgat
    13681 atgttcgtac aatttatagc ctgtttcaac cttagcttta tcattttcta attggatacg
    13741 ctgactgaaa tcttctaaaa ttaatggtag atatcgacca taatctccat aaatatttgt
    13801 ttccataaga aaccttccta ttctctctta agctccatta tatcatagaa acttaaggca
    13861 aactaaaaag gaatgagaat tttttcttcc catttccttc taaaatgttt atgttaagct
    13921 gattcgaatt gagaattata cagatcagca taaaaaccac tttgagacat caaaacatca
    13981 tgattgcctt gctcaatgat attcccatct ttcatgacca aaatcaagtc tgcgttttta
    14041 atcgttgata aacgatgtgc gataacaaat gatgttcttc cttccataag tttgtccata
    14101 gctttttgaa ttaactcttc tgtacgtgta tcaacagatg aagttgcttc atctaagata
    14161 agtaagggag aatttttcaa aagagctcga gcaatggtta ataattgctt ttgtccaact
    14221 gacaatgtca cagcgtcatc aaggacagta tcataccctt taggtagtgt cattataaaa
    14281 tgatgtatac cgactgcctt agcagcttct tgaacctcat catcactgat tgtcgtctgg
    14341 ttaaaaatca aattttcttt aatagttccc tcaaacaacc atgtatcttg aagaaccatc
    14401 gagaaagcat catgaacttg actacgtttc atgtcttgga tattgatgtc atctatggaa
    14461 atactacctg aattaacatc ataaaaacgc atcaataaat taacaattgt cgttttacct
    14521 gcgccagttg gtcctacaat agcaactttc tgaccaggct gtgcctccga agtaaagtca
    14581 tgaataatag tcttgttatc actataacca aacttgacat gttcaaagga cacatgcccc
    14641 ttaaacttag gaagatttaa cactttgtgt ttatcctctt gcatatttgt ttcatttaaa
    14701 aattcaaaaa cacgtcccat ggctgcacta gcagactgta attgtgtaat tccttgagca
    14761 atctgaccaa taggctgtgt aaaaatacga acataggtca taaaagcaac aattgtaccc
    14821 atagtaattg ttcctttgat agctaacatg gcaccaacca cacacaccat cacataacca
    14881 aaattaccaa taaactgcat cagaggcatc ataattccag agaaaaattg tgatttccac
    14941 atggaactgt gaagtttatc atttaatagt tgaaaatctg acttagcctg ctgtcttgca
    15001 ttatgactag taacaatgtt gtgtcctgag taaatttctt ctacatatcc agatatatta
    15061 gccaaattca cctgttgctt tttaaataaa ggctgactct tcaccataat gattgaagaa
    15121 agagcgaatc ctgaaaatac cgatataatc gccgttgttg ctaaatgcca atctgttttg
    15181 aacatcataa ttaatgaacc aaccaataac actgtagatg caaccatagt aactaaactt
    15241 tggttaaaag actgtgtcat caaatccaca tcatttgtaa cacgagataa agtatcacct
    15301 tgagtatggc tatcaaaata actcaaaggt attttattga ttttatcagc aatagcattg
    15361 cgcatacctt gagcaaattt ttgaataata gtcgtcacaa taaaacttgc tagataggat
    15421 actaatgcac ctaaaacata taatactgcc aattgccctg caatatgacc gattttggct
    15481 aagtcaatat taccagccaa gccttttacc ataagatccg tcatctcttt aagacggtct
    15541 ggtcctataa ctgttgtcaa gcttgataaa gttgctaaca caattgctaa cgcaaaaaga
    15601 agtttgtaac ctttgaggta cggagataaa ttagagaaaa aagacgtttt ttgtgattta
    15661 gttttcattt tccaattcct ccttagacaa ttgagaatag gctatctctt gatatacttc
    15721 attatttgat aacaattcct tatgtgttcc ttgcccaaca actttacctt gatctagcac
    15781 taaaatgaga tcagcatcca tgatagttga aatgcgttga gcgacaatta atttcgtcat
    15841 agcagctgtt ttttctttca gcacatggcg taaaatatga tctgttttat aatctaacgc
    15901 tgaaaatgag tcatcaaaaa tgagaatttc agggtgacgt gctaaagcac gtgcgattgc
    15961 caagcgttgt ttttgtcctc ctgaaaagtt actaccacct tgagcgactt ctgtatctaa
    16021 tcctttttct ttagcttcta taaattcttt agcttgcgct aattcgagtg cttgccaaat
    16081 ttgttcatca ctaagttcct ccctgccact ctctccaaaa atcatattag aacgtactgt
    16141 cccactaaag agaacagctt tctgtggaat atagccaatt ttattattca aatcattgtg
    16201 agagtattcc tttatatcaa tgccatcaag tttaattgac ccctctgtgg cgtcataaaa
    16261 acgtggaact aaattgacta aagtggattt tccagaacct gtagacccaa taaatgccac
    16321 tgtctgacca gtatctgctt tgaaagatac atgttcaata accgcttcag aatctttact
    16381 ataatgaaat gacacatctt gaaactcaat ttgacctttt ggtgcacttt ggatatgact
    16441 attttcttta aaagttactg ttggggctaa agttagtacc tcattaatac gtctagccga
    16501 tactaaagca cgaggcatga tgataaaaat agctaccatc atcataaatc ccataacaac
    16561 ttgcatagca taagatgaaa aaacaaccat gtttgaaaaa agatccactc gatcagtgat
    16621 agctgattta gcagccaata cagatacact tgttcctgta actttaggca cagaaatatc
    16681 attaatcaaa taagagccaa tccaataaat agctaaagtc aatccagatg aaaccactgt
    16741 catcacagga ttcataagcg acatcaatct ataaacaaaa aggttcaatt ttgttaaatc
    16801 gtcattttca cgtttgaact tatctgtttg atacttttca gcattgtagg cacgaataac
    16861 tctaacacca gagagtgcct ctctagtagt tgcattaagc gcatctgtaa aaccttggat
    16921 ttgacgttgt ctcggaaaag caagtaagag tagaacgcta agtaatatca taacgataat
    16981 aaccgctact ccaactgccc cagtccattg atcgctttta cccccaattt tagtcaaggc
    17041 ccatattgcc ataattggtc cacgggtaac aacctgcatt cccattacaa taaggatctg
    17101 tagctgagtc aagtcgttag ttgtgcgcgt taatagagag gggatagaaa atcgcttaat
    17161 ttctgcatca gaataatcca tcacatggtt aaaaatatct gaacgtaatc gactagtaaa
    17221 actagctgct gtcctagcac ctaaaaaacc taccattact gctactaaaa aactaccaaa
    17281 tgaaaaagca agcattttta gtccaggatc ccaaagagcc tctaactttg tacctttagt
    17341 ttgcaacata cgtgtgatat cagacatata ttctggagtc tttaggtcca tccaaaccgc
    17401 tagacacact aatatagtac tcgccacaat catcaaccat tctttaccat tgagtcgctt
    17461 gaaaatatta aacatcgcaa actcctttta ctttcatatt tatcctatta tcctaaaata
    17521 gttaatcaaa atcttccatc gccatatttt tagctaatgt tctaataacc tttttggtag
    17581 tacgtatctc ttctttagta atacctgcta acatggtact gtgcaatttt tctaagaaaa
    17641 tttcaaattg tgtagccttt tgtttaccta gatgagtcaa ataaaggtat ttcacacgct
    17701 tatctgtctt agatggaaca atcgcaataa atccattttt ttccatccgt tttaccaaat
    17761 tagacgctac agacttagaa atgtgtaaaa tttcttcagc atcttttata gacatatcct
    17821 tgtcaggatg tttatagaga tgcatcacta agtgtccttg tggacccgct aaatgttcaa
    17881 catcatactc ttttgcataa cgatctaaat acttttcaag ttgattaaca agtattcgtg
    17941 ctttttgaag aggattctcc ataataatac tcctttaata gttatc
//
LOCUS       DATRQA010000031        17537 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.031, whole genome shotgun sequence.
ACCESSION   DATRQA010000031 DATRQA010000000
VERSION     DATRQA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17537
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.031"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(23..1804)
                     /locus_tag="VBD27_000754"
     CDS             complement(23..1804)
                     /locus_tag="VBD27_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO7024784.1"
                     /translation="MLRALKEDFFLTLTSLKNQFWVYIGTGILIQTLVAYLVKGILSF
                     IFRRILILSNTPAVTKDNWTLLFNHPLALLLFILYIVILIGFIYTEFAIYTIAILKTE
                     FSLSIKKWLTIFPKKIKSLLGPQLIWVSIYLLLTIPLANLGLRSSILEHLKIPDFISG
                     ELTKTVFGKIGYSTLLLLICYLNLRLIYFLPLTILTDYNAKEALLESWKLSRGKRQWR
                     LLSKIILTSLFISVIGTVALAVVAGTFSFIDHSGNNFPLQATFYNLLKSILFAVTLLI
                     KCLIISHLLADIKQNKSIVQQWRAWKGVHHKQKFKHIACVILALAIGTTALKNAIALA
                     LLQDNISIKKELIAHRSDTSHGVENSIEALKAAHKAKADYSEMDVIMTKDHKLVVIHD
                     DNLKRLSGMNKDVSKLTLDQVTKIPIHQGHFASYIPSLAEFMKTAQSLDQKIMIELKP
                     YNQNLDIYADEFIKEFKELRLSTKHKVMSLNLTLIEKVEKKLPQLDTGYLIPLHWGTL
                     QNHNVDFYGIEEFSYNDWIAYLAQEYNKQLYVWTINRDNLMIRYLQSPVNGIITDKLN
                     LFKVINKDIKNSPNYYQRALQLIDSEG"
     gene            1974..2576
                     /locus_tag="VBD27_000755"
     CDS             1974..2576
                     /locus_tag="VBD27_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO7024785.1"
                     /translation="MKFLELNKKRHAVKHFNDKPVDFKDVRTAIEIATLAPSANNIQP
                     WKFVVVQEKKSALAEGLPESNCNQINQAQYVIALFTDTDLGQRSRKIARIGRRSLPDD
                     LIGYYMETLPPRYALYSEKQTGEYLSLNAGIVAMNLVLALTDQGISSNMILGFDKAIT
                     NDVLEIDKRFRPEILITVGYSDEKVEPSYRLPVDHIIEKR"
     gene            2623..4029
                     /gene="pepV"
                     /locus_tag="VBD27_000756"
     CDS             2623..4029
                     /gene="pepV"
                     /locus_tag="VBD27_000756"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEO7024786.1"
                     /translation="MTIDFRAEVDKRKDALMDDLINLLRINSERDDSQADAEHPFGPG
                     PVKALEFFLEMAERDGYETKNVDNYAGHFTFGQGEEELGIFGHLDVVPAGSGWDTDPY
                     EPVIKDNRLYARGSSDDKGPTMACYYALKIIKELGLPTSKKVRFVVGTDEESGWGDMD
                     YYFEHVGLPKPDFGFSPDAEFPIINGEKGNITEYLHFSGENKGAVRLHSFSGGLRENM
                     VPESATARFTSHLDQTTLGASLADFAGKHNLKAELSVEDGQYIATVYGKSAHGSIPQE
                     GVNGATYLALYLSQFDFEGPARAFLDVTANIIHEDFSGEKLGVAYEDDCMGSLSMNAG
                     VFQFDETNDDNTIALNFRYPQGTDAKTIQTKLEKLNGVEKVTLSDHEHTPHYVPMDDE
                     LVSTLLAVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPGDENTMHQANEYMPLE
                     NIFRSAAIYAEAIYELIK"
     gene            4126..4710
                     /locus_tag="VBD27_000757"
     CDS             4126..4710
                     /locus_tag="VBD27_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEO7024787.1"
                     /translation="MTDLEKIIKAIKSDSQNQNYTENGIDPLFAAPKTARINIVGQAP
                     GLKTQEAILYWKDKSGDRLRQWLGVDEETFYHSGKFAVLPLDFYYPGKGKSGDLPPRK
                     GFAEKWHPLILKEMPNVQLTLLVGQYAQKYYLGSSAHKNLTETVKSYKDYLPDYLPLV
                     HPSPRNQIWLKKNPWFEKDLIVDLQKIVADILKD"
     gene            4725..5459
                     /locus_tag="VBD27_000758"
     CDS             4725..5459
                     /locus_tag="VBD27_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024788.1"
                     /translation="MRDNHLHTHFSYDCQTAFEDYINGFTGEFITTEHFDLSNPYTGQ
                     DDVPDYSAYCQKIDYLNQKYGNRFKKGIEIGYFKDRESDILDYLKNKEFDLKLLSIHH
                     NGRYDYLQEEALKVPTKELLADYFNRMEFAIGRVEAHVLDHFDYGFRKLNLDVEDLKP
                     FETQLKRIFRKMLSKGLAFELNTKSLYLYGNEKLYRYALEILKQLGCKQYSIGSDGHI
                     PEHFCYEFDRLQCLLKDYQIDENHLI"
     gene            5628..9386
                     /locus_tag="VBD27_000759"
     CDS             5628..9386
                     /locus_tag="VBD27_000759"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEO7024789.1"
                     /translation="MKRKDLFGDKQTQYTIRKLSVGVASVATGVCIFLHSPQVFAEEV
                     SVSPATTAIAKSNINQVDNRQSTNLKDDINSNSETVVTPSDMPDTKQLVSDETDTQKG
                     VTEPDKATSLLEENKGPVSDKNTLDLKVAPSTLQNTPDKTSQAIGAPSPTLKVANQAP
                     QIENGYFRLHLKELPQGHPVESTGLWIWGDVDQPSSNWPNGAIPMTNAKKDDYGYYVD
                     FKLSEKQRKQISFLINNKAGTNLSGDHHIPLLRPEMNQVWIDEKYGIHTYQPLKEGYV
                     RINYLSSSGNYDHLSAWLFKDVATPSTTWPDGSNFVNQGLYGRYIDVPLKTNAKEIGF
                     LILDESKTGDAVKVQPNDYVFRDLANHNQIFVKDKDPKVYNNPYYIDQVQLKDAQQTD
                     LTSIQASFTTLDGVDKTEILKELKVTDKNQNAIQISDITLDTSKSLLIIKGDFNPKQG
                     HFNISYNGNNVTTRQSWEFKDQLYAYSGNLGAVLNQDGSKVEASLWSPSADSVTMIIY
                     DKDNQNRVVATTPLVKNNKGVWQTILDTKLGIKNYTGYYYLYEIKRGKDKVKILDPYA
                     KSLAEWDSNTVNDDIKTAKAAFVNPSQLGPQNLSFAKIANFKGRQDAVIYEAHVRDFT
                     SDQSLDGKLKNQLGTFAAFSEKLDYLQKLGVTHIQLLPVLSYFYVNEMDKSRSTAYTS
                     SDNNYNWGYDPQSYFALSGMYSEKPKDPSARIAELKQLIHDIHKRGMGVILDVVYNHT
                     AKTYLFEDIEPNYYHFMNEDGSPRESFGGGRLGTTHAMSRRVLVDSIKYLTSEFKVDG
                     FRFDMMGDHDAAAIELAYKEAKAINPNMIMIGEGWRTFQGDQGKPVKPADQDWMKSTD
                     TVGVFSDDIRNSLKSGFPNEGTPAFITGGPQSLQGIFKNIKAQPGNFEADSPGDVVQY
                     IAAHDNLTLHDVIAKSINKDPKVAEEDIHRRLRLGNVMILTSQGTAFIHSGQEYGRTK
                     RLLNPDYMTKVSDDKLPNKATLIEAVKEYPYFIHDSYDSSDAINHFDWAAATDNNKHP
                     ISTKTQAYTAGLITLRRSTDAFRKLSKAEIDREVSLITEVGQGDIKEKDLVIAYQTID
                     SKGDIYAVFVNADSKARNVLLGEKYKHLLKGQVIVDADQAGIKPISTPRGVHFEKDSL
                     LIDPLTAIVIKVGKVAPSPKEELQADYPKTQSFKGSKTVEKVNRIANKTSITPVVSNK
                     TDSYLTNEANLPKTGDKSSKILSVVGISILASLLALLGLSLKRNRHLK"
     gene            9521..10438
                     /locus_tag="VBD27_000760"
     CDS             9521..10438
                     /locus_tag="VBD27_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEO7024790.1"
                     /translation="MKNWEKPPLVMAGLVLGLLALGNLLEGYGTYFRYCLGLVALVFW
                     IFLIKGILKNKKESRKELSNPLIASVFTTFFMAGMILSTYILLFRSLGIWVAVLSKGV
                     WWLSFIALIIHMAIFSWKYLRHFSMANLFPSWSVLYVGIGVASLTAPISGQFTIGKIV
                     FWYGFIATLVLLPFLFIKAYKIGLPSAVKPNITTICAPMSLITAGYVNSFVSPNRGLL
                     LLLIVMAQFLYFFILFQVPKLLIGDFTPGFSAFTFPLVISATSLKLSIQHLSLPVDIQ
                     GLVHFEIGTTTLIVMIVMVRYIFFLRRTI"
     gene            complement(10480..10656)
                     /locus_tag="VBD27_000761"
     CDS             complement(10480..10656)
                     /locus_tag="VBD27_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEO7024791.1"
                     /translation="MVKKFAFAKGIATGVVATAATLAGAAFAIKKTIIEPEEEKIAFI
                     EENRKKAARKRVSH"
     gene            10767..11657
                     /gene="miaA"
                     /locus_tag="VBD27_000762"
     CDS             10767..11657
                     /gene="miaA"
                     /locus_tag="VBD27_000762"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO7024792.1"
                     /translation="MRKIKLIAVVGPTAVGKTALGIELAKTFNGEIISGDSQQVYQKL
                     DIGTAKASKEEQEQAYHHLIDVREVNENYSVYDFVKEAKVAIDTIISKGKIPIIVGGT
                     GLYLQSLFEGYHLGGEVNQETLKAYREKLESLSDEDLFEKLTEQSIVIPQVNRRRAIR
                     ALELAKFGNDLQNSESPYDVLLIGLNDDRQVLYDRINRRVDLMIDNGLLDEAKWLYDN
                     YPSVQASRGIGYKELFPYFSKQIPLEEAVDKLKQNTRRFAKRQLTWFRNRMNVEFIMV
                     GEENYQQKIKRKVSDFLSSK"
     gene            11748..12986
                     /gene="hflX"
                     /locus_tag="VBD27_000763"
     CDS             11748..12986
                     /gene="hflX"
                     /locus_tag="VBD27_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO7024793.1"
                     /translation="MIETKEEQERVILVGVELQDTENFEMSMEELASLAKTAGANVVN
                     HYYQKRDKYDSKSFIGSGKLEEIKAIVEADEIDTVVVNNRLTPRQNSNLEAELGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQISDIERQLKIVEKNRETVRERRVDSTTFKIGLIGYTNAGKSTIMNVL
                     TDDKQYEANELFATLDATTKQIYLQNQFQVTLTDTVGFIQDLPTELVAAFKSTLEESR
                     HVDLLLHVIDASDPNHEEHEKVVMEILKDLDMIDIPRLAIYNKMDVTEQLNATTFPNV
                     RIAAKKQGSRDLLRRLIVDEIRHIFDEFSIRVHQNQAYKLYDLNKIALLDTYTFEEEY
                     ENITGYISPKQKWKLEEFYD"
     gene            12979..13626
                     /locus_tag="VBD27_000764"
     CDS             12979..13626
                     /locus_tag="VBD27_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEO7024794.1"
                     /translation="MIDYIDLALTYGGFTSLDKVYLEKKLDGLSKQQRLDFITPPPSV
                     INAYFAEIYQKQGPEAATDYYFDLSKALGLFPKHLSFDEEKPFIRLNLSGKSFGFAYL
                     NDQEEASVFSEVKEVITPQLLLEIAQIFPQYKVYQDRSGIRMAKIDFDETESQNITPE
                     TSLLGNVLQLKKDIIKITSFNQEELLELVKTKSGEYYYSSQGRESVIYIDTKSTN"
     gene            13668..14597
                     /locus_tag="VBD27_000765"
     CDS             13668..14597
                     /locus_tag="VBD27_000765"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEO7024795.1"
                     /translation="MEIQFLGTGAGQPAKARNVSSLVLKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKVKKIFITHMHGDHVFGLPGFLSSRAFQANEEQTDLDIYGPVGIKSFV
                     MTGLRTSGSRLPYRIHFHEFDESSLGKIMETDKFTVYAEKLDHTIFCMGYRVVQKDLE
                     GTLDAEALKLAGVPFGPLFGKVKNGENVTLEDGREIIAKDYISEPKKGKVITILGDTR
                     KTDASIRLALGADVLVHESTYGKGDERIAKSHGHSTNMQAADIAKQANAKRLLLNHVS
                     ARFMGRDCWQMEEDAKTIFSNTHLVRDLEEVGI"
     gene            14599..15360
                     /locus_tag="VBD27_000766"
     CDS             14599..15360
                     /locus_tag="VBD27_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO7024796.1"
                     /translation="MARTILITGASGGLAQAIINQLPQDDHLIVTGRSREKLEKLYGK
                     RPNTLCLSLDITNDNAVTNMIEKIYVEFGQIDILINNAGFGSFKEFWDYSDEEVKDMF
                     AVNTFATMSIARQIGHKMSLVKSGHIVNIASMAGLIATSKASVYGASKFAVVGFLNAL
                     RLELAEKNVYVTSVNPGPIKTGFFAQADPSGDYLASIGRFVLTPEKVAKKVVSILGKN
                     KRELNLPFILAFAHKYYSLFPKTADYFARKVFNYK"
     gene            15357..>17537
                     /gene="recJ"
                     /locus_tag="VBD27_000767"
     CDS             15357..>17537
                     /gene="recJ"
                     /locus_tag="VBD27_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO7024797.1"
                     /translation="MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTS
                     AELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIENNETILVYGDYDADGMTSASIMK
                     EALDMMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQDVSLIITVDNGVAGHEAIAYAQ
                     NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPT
                     EMLDLVAIGTIADMVSLTDENRIMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEET
                     IGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKNEERKEIVQTIFDQA
                     MQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEAL
                     DIFQAFDQHRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTI
                     DSELRFDELSLDTVRDFEKLAPFGMDNKKPVFLLKDFKVSQARVMGQNGAHLKLKLEQ
                     DGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVDGIQLFD
                     IRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNY
                     FISGYGTRDQFASLYKTIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVT
                     ITEGIMTVNKEAEKRDISESQIYQELKETVKFQELMALGTPKEIY"
BASE COUNT         5942 a         2668 c         3417 g         5510 t
ORIGIN      
        1 tctactaagc tttttattta atttaaccct cgctatcaat aagttggaga gctctttgat
       61 aataattagg gctatttttg atatctttat taatgacctt gaaaagattg agtttatctg
      121 tgatgattcc attaactggg ctttgtaaat aacgtatcat aaggttatca cggttaatag
      181 tccaaacata gagctgttta ttatattctt gggcgagata tgcaatccaa tcattgtatg
      241 aaaattcctc aatcccataa aagtcaacgt tatgattttg aagcgttccc caatgtagtg
      301 ggattaggta acctgtatca agttgtggca attttttctc aaccttttca atgagtgtca
      361 agttgagaga cattacttta tgcttggttg atagacgaag ctctttaaat tctttgataa
      421 attcatccgc atagatatcg aggttttgat tatacggttt caattcaatc atgatttttt
      481 gatctagtga ctgagcggtc ttcataaatt ctgccaatga tggaatgtaa ctagcaaaat
      541 gcccttggtg aattgggatt tttgtaactt gatctaaagt tagtttagaa acatctttgt
      601 tcataccaga gagtcgtttg agattatcat catgaatgac tactaattta tgatcttttg
      661 tcatgataac atccatctct gaataatctg cttttgcttt atgggctgct tttaatgctt
      721 caatagaatt ttcaacgcca tgtgatgtat cactacgatg ggctattaat tcttttttaa
      781 ttgaaatgtt atcttgtaag agagccagag ctatagcatt ttttaaggca gtggtaccaa
      841 tagctagagc tagaataaca caagcaatgt gtttaaattt ttgcttatgg tggacgcctt
      901 tccaagctct ccattgttga acaatacttt tattttgttt aatatctgct aataggtgac
      961 ttatgattaa acatttaata agaagtgtta cggcaaaaag tattgatttt aaaaggttat
     1021 agaaagtggc ttgaagagga aaattgtttc cagaatgatc aatgaatgaa aatgtaccag
     1081 caacaacagc tagtgcaact gttccgatta cagatataaa taaactggtt aaaataattt
     1141 tacttagaag acgccactga cgctttccgc ggctaagttt ccaactctct aaaagtgctt
     1201 ctttagcatt gtagtcagtt aaaatggtca gcgggagaaa ataaattaat cgaaggttta
     1261 agtaacaaat aagcagtaaa agggtagagt aacctatctt tccaaagact gtctttgtta
     1321 actcgccaga aataaagtca ggaattttaa gatgctctaa tatacttgac cttagaccaa
     1381 ggttagctag aggaattgtc aaaagtaagt agattgatac ccaaattagc tgtggtccta
     1441 aaagtgattt tattttttta ggaaagattg ttagccattt tttgatactg agagaaaatt
     1501 cagttttcag tatggcaata gtatagatag cgaactcagt atagatgaac ccaattagga
     1561 ttacgatata aagtataaag agtaataaag ctaagggatg attaaaaagc aaagtccaat
     1621 tgtctttagt cactgcggga gtatttgata atatcaatat ccgacgaaag ataaaactca
     1681 gaattccttt tactaaataa gcaactaaag tttggattaa gataccagtt ccaatgtaaa
     1741 cccaaaattg attttttaaa gaggttaagg ttagaaaaaa atcttctttt aatgctcgta
     1801 gcatgaatag ctcctttttt atagtcatta gatattgtac cacaagtttg tttgtttttt
     1861 atggtagttt tcaaaccggt ttcacttttt tatcaatttt gccttcaaaa gctgaaacgc
     1921 attttaaaaa gtgatagaat agtagggaaa atcaaataga ataggaggtt tttatgaaat
     1981 ttctagaatt aaataagaag cgccatgctg tgaaacattt taatgataag ccagtcgact
     2041 ttaaagatgt tagaactgct attgaaattg cgacattagc gccatctgct aataatattc
     2101 agccttggaa gtttgttgtt gtccaagaaa aaaaatctgc tctagcagaa ggtttaccgg
     2161 aaagtaattg taaccaaatt aatcaagcgc agtatgttat tgctttattt acagatacag
     2221 atttaggaca gagatcacgg aaaatagctc gtattggtcg tagatctctt cctgatgatt
     2281 taattggtta ttatatggaa actctaccac cacgctatgc tttatatagt gaaaaacaaa
     2341 ctggagagta tctctcttta aatgcaggta tcgttgctat gaatttagta cttgccttga
     2401 cagatcaagg tattagttct aatatgattt tagggttcga taaagcgatt accaatgatg
     2461 tcttagagat agataaacgt tttagaccag aaatattaat tactgttggt tatagtgatg
     2521 aaaaggttga gcctagttat cgtttgccag ttgatcatat tattgaaaaa cgttaatttt
     2581 taagcacaaa ctacataata ttttatagaa ggagttgccg taatgacaat tgattttaga
     2641 gcagaagtag acaaacgcaa agacgctttg atggacgatt taataaatct tcttcgtatt
     2701 aactcggagc gtgatgattc acaagctgat gcagagcatc catttggtcc tggacctgta
     2761 aaagccttgg aatttttcct tgagatggct gagcgagatg gctacgaaac aaaaaacgta
     2821 gataattatg cgggacactt tacatttggt caaggcgaag aagaactagg tatttttggg
     2881 catttagatg ttgttcctgc aggcagcggt tgggatacag acccttatga accagttatc
     2941 aaggataacc gtttatatgc tcgagggtca tcagacgata agggaccaac aatggcatgt
     3001 tattatgctc taaaaattat taaagagctt gggctaccaa catctaagaa agttcgtttt
     3061 gttgtaggaa ctgatgaaga atctggatgg ggagatatgg attactactt cgaacatgtt
     3121 ggtcttccaa aacctgattt tggtttctct ccagatgcag aatttccaat tataaatggt
     3181 gaaaaaggta atatcactga atatttacat ttttcaggag aaaataaggg agctgttaga
     3241 ctgcatagct tttctggcgg acttcgcgaa aacatggttc cagaatcagc cacagcacgt
     3301 ttcacaagtc atttagatca gacaacactt ggagctagtc tagctgattt tgcaggtaaa
     3361 cataatttga aagcagaatt atctgtagaa gatgggcagt atatagctac agtttatggt
     3421 aaatctgctc atggttcaat accacaagaa ggtgttaatg gggcgactta tttagctctt
     3481 tatctaagtc aatttgattt tgaaggtcct gctcgtgctt tcttagatgt cacagccaac
     3541 attattcacg aagacttctc aggtgaaaaa cttggagtag cttatgaaga tgactgtatg
     3601 ggatcgttga gcatgaatgc aggtgtcttc cagtttgatg aaactaatga tgataatact
     3661 atcgctctta atttccgtta cccacaaggg acagatgcta aaactatcca aactaagctt
     3721 gagaaactta acggagttga aaaagtgact ctttctgacc atgaacacac accacactat
     3781 gtacctatgg acgatgaatt agtatcaacc ttactagctg tctatgaaaa gcaaactggt
     3841 cttaaaggac atgaacaggt tattggtggg gggacatttg gtcgcttact tgaacggggt
     3901 gttgcatacg gtgccatgtt cccaggagat gaaaacacta tgcatcaagc taatgagtac
     3961 atgcctttag aaaatatttt ccgttcggct gctatctacg cagaagctat ctatgaatta
     4021 atcaaataaa ataatcctta aactaaatat gtgatcaatg ataaagggtg gtgaagacat
     4081 gaaagtgtct ttgcctcttt tcataaggtt agatttggag actttatgac tgacttggaa
     4141 aaaattatta aagcaataaa aagtgattca cagaatcaaa attatacaga aaatggtatt
     4201 gatcctttgt ttgctgctcc taaaacagct aggatcaata ttgttggaca agcacctggt
     4261 ttaaaaactc aagaagcaat actctattgg aaagataaat ctggagatcg tctacgccag
     4321 tggcttggag ttgatgaaga gacattttac cattctggaa aatttgctgt tttgccttta
     4381 gatttttatt acccaggcaa aggaaaatca ggagatttac cccctagaaa aggttttgcg
     4441 gagaaatggc accctcttat tttaaaagaa atgcctaatg ttcaattgac cttgctagtt
     4501 ggtcaatatg ctcagaaata ttatcttgga agctccgcac ataaaaatct aacagaaaca
     4561 gttaaatctt acaaagacta tctacccgat tatttacccc tggttcaccc atcaccgcga
     4621 aatcaaattt ggctaaagaa gaatccatgg tttgaaaaag atctaatcgt tgatttacaa
     4681 aagatagtag cagatatttt aaaagattaa ggataggagt tagtatgaga gataatcatc
     4741 tacacacgca tttttcctat gattgtcaaa cggcatttga ggactatatt aatggtttta
     4801 caggtgaatt tatcacaaca gaacattttg atttatcaaa tccttacacc ggtcaagacg
     4861 atgttcctga ttatagtgct tattgtcaaa aaatagatta tcttaatcag aaatatggaa
     4921 atcgatttaa aaaaggaatt gaaatcggtt attttaaaga tagggaatca gatattttag
     4981 attatttaaa aaataaagaa tttgatttaa aactattgtc aatccatcat aatggtaggt
     5041 atgattatct gcaagaagaa gctctgaaag taccaacaaa ggagctttta gcagattact
     5101 ttaatcgtat ggaatttgcc ataggccgtg tggaagcgca cgttttagat cactttgatt
     5161 atggttttcg taagttaaac ttagatgtag aagatttaaa accgtttgaa acgcaattga
     5221 agcgcatttt cagaaagatg ttatctaagg ggttagcttt tgaactaaat accaaatccc
     5281 tttatctata tgggaatgaa aaactttatc gctatgcttt agagatacta aaacagcttg
     5341 gttgtaaaca atactctata ggctctgacg gtcatattcc tgaacatttt tgttatgaat
     5401 ttgatagact tcaatgtctg ctaaaggact atcaaattga tgaaaatcat ttgatatgag
     5461 gaaatttttg ataaaaaagc taggcaatat tgcttagctt ttttgtaatg tcattgatag
     5521 ttttagtgaa aatttcaaaa aaataaagaa atcacttact tgttgcaagc gcttgcgtaa
     5581 attgttatga ttttattggt aacaattcat taaaaaagga gaatgatatg aaaagaaaag
     5641 acttatttgg tgataaacaa actcaataca cgattagaaa gttaagtgtt ggagtagctt
     5701 cagttgcaac aggggtatgt atttttcttc atagtccaca ggtatttgct gaagaagtaa
     5761 gtgtttctcc tgcaactaca gcgattgcaa agtcgaatat taatcaggtt gacaaccgac
     5821 aatctactaa tttaaaagat gacataaact caaactctga gacggttgtg acaccctcag
     5881 atatgccgga taccaagcaa ttagtatcag atgaaactga cactcaaaaa ggagtgacag
     5941 agccggataa ggcgacaagc ctgcttgaag aaaataaagg tcctgtttca gataaaaata
     6001 ccttagattt aaaagtggca ccatctacat tgcaaaatac tcccgacaaa acttctcaag
     6061 ctataggtgc tccaagtccg accttgaaag ttgctaatca agctccacag attgaaaatg
     6121 gttactttag gttacatctt aaagaattgc ctcaaggtca tcctgtagaa agcactgggc
     6181 tttggatatg gggagatgtt gatcaaccgt ctagtaattg gccaaatggt gctatcccta
     6241 tgactaatgc taagaaagat gattacggtt attatgttga ttttaaatta tctgaaaaac
     6301 aacgaaaaca aatatctttt ttaattaata acaaagcagg aacaaattta agcggcgatc
     6361 atcatattcc attattacga cctgagatga accaagtttg gattgatgaa aagtacggta
     6421 tacatactta tcagcccctc aaagaagggt atgtccgtat taactatttg agttcatctg
     6481 gtaactatga ccacttatca gcatggctct ttaaagatgt tgcaaccccc tcaacaactt
     6541 ggccagatgg tagtaatttt gtgaatcaag gactatatgg aaggtatatt gatgtaccac
     6601 tgaaaactaa tgccaaagag attggttttc taatcttaga tgaaagtaag acaggagatg
     6661 cagtgaaagt tcaacccaac gactatgttt ttagagattt agctaaccat aaccaaattt
     6721 ttgtaaaaga taaggatcca aaggtttata ataatcctta ttacattgat caagtgcagc
     6781 taaaggatgc tcaacaaact gatttaacaa gtattcaagc aagttttaca actctagatg
     6841 gggtagataa aactgaaatt ttaaaagaat tgaaagtgac agataaaaat caaaatgcta
     6901 tacaaatttc tgatatcact ctcgatacta gtaaatctct tttaataatc aaaggcgact
     6961 ttaatcctaa acaaggtcat ttcaatatat cttataatgg taacaatgtc acgacaaggc
     7021 aatcttggga atttaaagac caactttatg cttatagtgg aaatttaggt gcagttctca
     7081 atcaagatgg ttcaaaagtt gaagccagcc tctggtcacc gagtgctgat agtgtcacta
     7141 tgattattta tgacaaagat aatcaaaaca gggttgtagc gactaccccc cttgtgaaaa
     7201 ataataaagg tgtttggcag acgatacttg atactaaatt aggtattaaa aactatactg
     7261 gttactatta tctttacgaa ataaaaagag gtaaggataa ggttaagatt ttagatcctt
     7321 atgcaaagtc attagcagag tgggatagta atactgttaa tgacgatata aaaacggcta
     7381 aagcagcttt tgtaaatcca agtcaacttg gacctcaaaa tttaagtttt gctaaaattg
     7441 ctaattttaa aggaagacaa gatgctgtta tatacgaagc acatgtaaga gacttcactt
     7501 ctgatcaatc tttggacgga aaattaaaaa atcaacttgg tacctttgca gccttttcag
     7561 agaaactaga ttatttacag aaattaggag ttacacacat tcagctttta ccggtattga
     7621 gttattttta tgttaatgaa atggataagt cacgctcaac agcttacact tcctcagaca
     7681 ataattacaa ttggggctat gacccacaga gctattttgc tctttctgga atgtattcag
     7741 agaaaccaaa agatccatca gcacgtatcg ccgaattaaa acaattaata catgatattc
     7801 ataaacgtgg catgggggtt atacttgatg tcgtctataa tcacactgca aaaacttatc
     7861 tctttgagga tatagaacct aattattatc actttatgaa tgaagatggt tcaccaagag
     7921 aaagttttgg agggggacgt ttaggaacca ctcatgcaat gagtcgtcgt gttttggttg
     7981 attccattaa atatcttaca agtgaattta aagttgatgg tttccgtttt gatatgatgg
     8041 gagatcatga tgcggctgcg attgaattag cttataaaga agctaaagct attaatccta
     8101 atatgattat gattggtgag ggctggagaa cattccaagg cgatcaaggt aagccggtta
     8161 aaccagctga ccaagattgg atgaagtcaa ccgatacagt tggcgtcttt tcagatgata
     8221 ttcgtaatag cttgaaatct ggttttccaa atgaaggtac tccagctttc atcacaggtg
     8281 gcccacaatc tttacaaggt atttttaaaa atatcaaagc acaacctggg aattttgaag
     8341 cagattcgcc aggagatgtg gtgcagtata ttgctgcaca tgataacctt accttgcatg
     8401 atgtgattgc aaaatcaatt aataaagacc ctaaggtagc tgaagaagat attcatagac
     8461 gtctgcgttt aggaaatgta atgattttaa catctcaagg gacagcattc attcattctg
     8521 gtcaagagta tggtcgtacg aagcgtttac ttaaccctga ttacatgaca aaagtttcag
     8581 atgacaaatt gcctaataaa gcaacactta ttgaagctgt taaagaatac ccatatttta
     8641 ttcatgattc atatgattct tcagatgcca ttaatcattt tgattgggca gcagccacag
     8701 ataataacaa acacccaatt tcaacgaaaa cacaggccta tacagcaggt ttaatcacat
     8761 taaggcgttc aacagatgct ttccggaaat tgagcaaagc agaaattgat cgtgaggtta
     8821 gcttgattac agaggtaggt caaggtgata ttaaagaaaa agatttggtt attgcttacc
     8881 aaacaataga ttctaaaggc gatatttacg cagtatttgt taatgctgat agtaaagcta
     8941 gaaacgtttt actaggtgaa aaatataaac accttttaaa agggcaagta attgttgatg
     9001 ctgatcaagc ggggattaaa ccaatctcaa ctcctagagg tgttcatttt gaaaaagata
     9061 gtttgctgat tgatccatta acagcaattg tgattaaagt tggcaaagtt gctcctagcc
     9121 ctaaggagga attgcaagca gattatccca aaacacaatc tttcaaggga tctaaaacgg
     9181 tagaaaaagt aaatagaata gctaataaga cctcaataac tcctgtagtt tctaataaga
     9241 ccgattcata tctgacaaat gaagctaatt tgccaaaaac tggagataag tcatcaaaaa
     9301 tactaagtgt agtaggaata agcattctag caagtctact tgctctacta ggtctctctt
     9361 taaagaggaa tcgtcactta aaataaaagc aaactttaga agagcttaaa aaagaagtta
     9421 gtcaatgggc tagcttcttt tcattaagtt aataaaatca cttatcagca aaatctaatt
     9481 gtgttataat ggatgtaaac gtttttttat gaggtatatg atgaaaaatt gggaaaaacc
     9541 accattagtg atggcgggat tagtattggg gctattagct ttggggaacc ttctagaggg
     9601 atatggcacc tattttcgct attgtttagg gctagtagca cttgtttttt ggattttttt
     9661 aatcaaagga atattaaaaa ataaaaaaga aagcaggaag gagttaagca atcctttgat
     9721 tgcttctgtc tttactactt tttttatggc aggtatgatt ttatcgacct atatcttatt
     9781 atttagaagt ttaggaatat gggtagcagt tctgtcaaaa ggtgtatggt ggttgtcatt
     9841 tattgcgtta atcattcata tggctatttt ttcatggaaa tatttgagac acttttcgat
     9901 ggctaatctt tttccttctt ggtctgtcct ctatgttggt ataggggtag ctagcctgac
     9961 agcaccaatt agtggacaat tcactattgg gaaaatagtt ttttggtatg gttttattgc
    10021 aactttagta ttattaccat ttctattcat taaagcatat aaaatcgggc taccttcggc
    10081 agttaagcct aatataacta ctatttgcgc tccaatgtcg ctaatcacag cgggatatgt
    10141 taatagtttt gtgtcaccaa atagaggttt attattgcta ttaatcgtaa tggcacaatt
    10201 tctttatttt ttcattcttt ttcaggtgcc taaattactg attggtgatt ttacaccagg
    10261 tttctcagcc tttactttcc cccttgttat ctcagctacc tcacttaaat taagtattca
    10321 gcatctatca ttaccagttg atatacaggg gctagtccac tttgaaattg ggacaacaac
    10381 tcttattgtt atgatagtaa tggtacgtta tattttcttt ttaaggcgaa caatttaata
    10441 taaaataact cccttactag ctagtaaggg agttattgtt taatgagaga cacgtttacg
    10501 tgcagctttt ttacgatttt cttcgataaa agctattttt tcttcctcag gttcaataat
    10561 tgtttttttg atagcaaatg cagcacctgc aagagtagca gctgttgcaa cgactcctgt
    10621 tgctattcct ttagcaaatg cgaatttttt aaccataata attctccaat ctatgatgaa
    10681 gactctgctt ctatctatga tataattatg gtaacgaaat actagttaaa tttcaagtaa
    10741 aaacctttat agaattgaga agtatcatga gaaaaatcaa gcttatagca gttgttggtc
    10801 caactgcggt tgggaagaca gcattaggaa ttgaattagc taaaactttc aatggcgaaa
    10861 tcattagtgg cgatagtcag caagtttatc agaagttaga tatcggaacg gctaaagcta
    10921 gtaaagaaga gcaagaacaa gcttatcatc acttgattga tgttcgtgaa gtaaatgaga
    10981 attattcggt ttatgatttt gtcaaagagg caaaagtagc tattgatacg attatttcta
    11041 aggggaaaat ccctattatt gttggaggta ctggcctata tttacagagt ttgtttgagg
    11101 gctatcattt aggaggcgaa gtcaatcaag aaactcttaa ggcctatcgt gaaaaattag
    11161 agtctctaag tgatgaagat ttatttgaaa agttgacaga acaatccatt gttataccgc
    11221 aagttaatcg tcgacgtgcc atccgtgcat tagaattggc gaagtttggg aatgatttgc
    11281 aaaatagtga aagtccgtat gatgttttat taattggatt gaatgatgat cgtcaggtgc
    11341 tctatgatcg tatcaataga cgagttgatt tgatgataga caatggtctc ttagatgaag
    11401 ctaaatggct atacgataac taccctagtg ttcaagcaag tagaggaatt ggctataaag
    11461 aattatttcc ttacttttca aagcaaatac cattagaaga agctgttgat aaactaaaac
    11521 aaaataccag acgttttgca aaacgccaat taacatggtt tagaaaccgt atgaatgtcg
    11581 aatttattat ggttggtgag gaaaattatc aacaaaaaat caagcgaaaa gtcagtgatt
    11641 ttttatcatc aaagtaaata aagccgtttc tcgcatttaa agggcattat gttataataa
    11701 agattacgta tttagaacaa gaaaaaaata ttgaaaggag attataggtg atagaaacta
    11761 aagaagagca agaacgtgtt attttagttg gggtagaact tcaagatact gagaattttg
    11821 aaatgtccat ggaagaactt gctagcttag ctaagacagc aggagcaaat gttgttaatc
    11881 attattatca aaaaagagat aaatatgata gtaaatcatt tatcggatct ggtaaattag
    11941 aggagataaa agctattgta gaagcagatg agattgatac cgttgtggtt aataatcgtt
    12001 tgactccgcg acaaaactct aatttggaag ctgaacttgg tgttaaagtt attgaccgta
    12061 tgcaacttat tttagatatc tttgctatgc gtgctagaag tcatgaagga aaattacagg
    12121 ttcatctagc gcaattgaaa tatatgttac cacgcttagt tggacaaggt attatgctta
    12181 gtcgtcaagc aggaggaatc ggaagccgag gtccaggaga atctcagtta gagttgaatc
    12241 gtcgttccat tcgtcatcaa atttctgata tcgaaagaca attgaagatt gtagaaaaaa
    12301 atcgcgagac agttcgagaa agacgtgttg attcgacaac ctttaaaatt ggattaattg
    12361 gatatacaaa tgctggcaag tcaactatta tgaatgtttt gaccgatgat aagcaatatg
    12421 aagccaatga attatttgca acgttagacg ctacaaccaa gcagatctat ttacaaaatc
    12481 aatttcaagt cactttaaca gatactgttg gttttattca agatttacct acagagttag
    12541 ttgcggcttt caagtctacc ttagaagaaa gtcgacatgt tgatttatta cttcatgtta
    12601 ttgatgctag tgaccctaat catgaagaac acgaaaaagt tgttatggag attcttaaag
    12661 acttagatat gatagatatt cctcgtttag cgatatacaa caagatggat gtcactgagc
    12721 agctgaatgc aacaactttt cctaatgtga gaattgcagc caaaaaacaa gggagtaggg
    12781 atttattacg ccgtcttatt gtggatgaaa tacgacatat ctttgacgaa ttttcgatta
    12841 gagtgcatca aaatcaagca tataagctct atgatctcaa taaaatagca ttgcttgaca
    12901 cctacacctt tgaagaagaa tacgagaata ttacaggcta tatttctcct aaacaaaaat
    12961 ggaagctgga agaattttat gattgattat attgatttgg ctttgactta tggtggtttt
    13021 acgagcttag ataaagttta tctagagaaa aaactagatg gcctctctaa gcaacaacga
    13081 cttgatttta tcactccacc tcctagtgtg attaatgctt attttgctga aatatatcaa
    13141 aagcaagggc cagaagcggc aactgattac tattttgatc tttccaaagc cttgggcttg
    13201 tttccaaaac atttatcgtt tgatgaagaa aaacctttta ttcgtttgaa tttatcagga
    13261 aaatcatttg gttttgctta cctaaatgat caagaagaag cgagtgtttt ttctgaagtt
    13321 aaagaagtca ttacaccgca gttactctta gaaattgcac agatttttcc tcaatataaa
    13381 gtctatcaag atcggtctgg tatcagaatg gcaaaaatag attttgacga aacagaatct
    13441 caaaatatta caccagaaac aagtttacta gggaatgtct tacaacttaa aaaagatatt
    13501 attaaaataa ctagtttcaa tcaagaagag ctattagagt tggttaaaac aaagtcaggt
    13561 gaatactatt attctagtca aggacgtgag tcagttattt atattgatac taaatctact
    13621 aattaaattt attaagttat ggaaggatta taaaaaagtc aacaagtatg gaaattcaat
    13681 ttttaggaac tggagctggt caaccagcga aagctcgtaa tgtttcaagt ttagtcttga
    13741 aattattaga tgaaattaac gaggtttgga tgtttgattg tggagagggt acacagcgtc
    13801 aaattttaga aaccacgatt aaacctcgta aagtcaaaaa aatttttatc acgcacatgc
    13861 acggagacca tgtttttggt cttccaggtt ttttatccag tcgggctttt caagccaatg
    13921 aagaacagac agatttagat atttatggac cggtcggtat aaaaagtttt gttatgactg
    13981 gcttaaggac atcaggttca cgtttacctt atcgaattca ttttcacgag tttgatgaat
    14041 catcactcgg aaaaattatg gaaacagata aatttaccgt ttatgcagaa aagctagatc
    14101 atactatctt ttgtatggga tatcgtgttg tgcaaaaaga ccttgagggc actttagatg
    14161 ctgaagcatt gaaactagca ggtgttccat ttggtcccct ttttgggaaa gtcaaaaatg
    14221 gtgagaatgt taccttagaa gatggtagag aaattattgc taaagattat atttctgaac
    14281 cgaaaaaggg taaagtaatc actattttgg gagatactag aaaaacagat gcaagtatta
    14341 ggttagcttt gggcgctgac gttttagtgc acgaatctac ttatggtaag ggtgatgagc
    14401 gaattgctaa atcgcatggg cattcaacca atatgcaagc agctgatatc gctaaacaag
    14461 caaatgctaa acgactatta ttaaatcatg ttagtgctcg ttttatgggg cgagattgtt
    14521 ggcaaatgga agaagatgca aagacgattt tttcaaatac ccatcttgtt agagatttgg
    14581 aagaagtagg tatataaaat ggcaagaacg atactgatta caggagcttc tggtggttta
    14641 gcacaggcaa ttattaacca attacctcaa gacgaccatt taattgtcac agggagatca
    14701 agagaaaaac ttgaaaagct ttatggaaag agaccaaaca ccttatgttt atcactagac
    14761 ataactaatg acaatgctgt aacgaatatg attgaaaaaa tttatgttga atttggtcaa
    14821 attgatattt taattaataa tgctggattt ggttctttta aagaattttg ggattatagt
    14881 gatgaagaag tcaaagacat gtttgctgtg aatacatttg caacgatgtc tatagctcgt
    14941 cagataggac ataagatgtc tcttgtaaaa tctggtcata tcgttaatat tgcaagcatg
    15001 gctggcctca tagcaacaag taaagctagc gtttatgggg ctagtaagtt tgctgtcgtt
    15061 ggatttttga atgctcttcg tttagaatta gcagaaaaaa atgtttatgt gacaagtgta
    15121 aaccctggac caatcaaaac aggatttttt gctcaggctg atccaagtgg tgattattta
    15181 gcaagtatag gtcgttttgt tctaacacca gaaaaagtag ctaaaaaagt agtttctatt
    15241 cttggaaaaa ataagagaga attaaatctt ccttttatat tagcttttgc gcataaatat
    15301 tatagtttat ttccaaaaac agctgattat tttgccagaa aggtattcaa ttataaatga
    15361 tatctgctaa gtatagttgg gtcctcaata accaaaagcc agacgctggc ttttttgagg
    15421 cttcaaaaaa agaaaagata agtgaagcgg tggcttcctt aatttattcg cgaggtatta
    15481 agacatcagc ggaattacat cattttttac aaactaattt ggaaaatctc catgatcctt
    15541 atttgttaaa tgatatggat aaagctgtta atcgtattag acgagctatt gaaaataatg
    15601 aaactatttt agtttatgga gactatgatg ctgatggaat gacatcagca tcaatcatga
    15661 aagaagcatt agatatgatg ggagctgaag tccaggttta tctcccaaat cgctttacag
    15721 atggttacgg tcccaatcaa agtgtttata aatattttat tgaacaacaa gatgtttcat
    15781 taatcattac ggttgataat ggtgtggcag ggcatgaagc aattgcatat gctcaaaatc
    15841 aaggtgtgga cgtggtagtt actgatcatc acagtatgcc agcagattta ccttgtgctt
    15901 atgctattat tcacccagaa catcctgatg ctaattatcc atttccttat ttagcagggt
    15961 gtggtgttgc tttcaaagta gcttgtgctc tcttagaaac aatcccgaca gaaatgttgg
    16021 atttggttgc tattgggacg attgcagata tggttagcct aactgatgaa aatcgtatca
    16081 tggtaaaagc tggacttgaa gttatgaaag atagtgaaag aataggctta caggagctaa
    16141 tatctttatc aaatattgat ttaaaaacac taaatgaaga aactattgga tttaaaattg
    16201 ctccacaatt aaacgcttta ggaagacttg atgaccctaa tccagctatt gaattactaa
    16261 cagggtttga tgatgaagaa tctcaagcaa tagcccaaat gattgatcaa aaaaatgaag
    16321 agcgcaaaga aatcgttcaa actatctttg accaagctat gcaaatgctt gatcaaacaa
    16381 agcctgtaca agttttagca aaagaaaatt ggcatccagg ggttttaggt attgtggcgg
    16441 gtcgtatttt agagagaact ggacaacctg ttattgtttt gaatattgaa gatggtattg
    16501 ctaaaggaag tgccagaagt gtagaagcac ttgatatttt tcaagctttt gaccagcatc
    16561 gagaattatt cattgctttt ggaggtcaca gcggagcagc aggcatgact ttagaggaaa
    16621 gtaaggtagg tgatctttct caagttttat gtgactatat ctctaaaaaa caattggaca
    16681 tgagtcagaa aaaaactctg acaattgata gtgagctacg ttttgacgaa cttagtttag
    16741 acactgttag agattttgaa aagttagcgc catttggaat ggataataaa aaaccagtct
    16801 ttttgttaaa agattttaaa gtgagtcaag ccagagtcat ggggcaaaac ggtgctcatc
    16861 tgaaattaaa actagaacaa gatggacaag cacttgattt ggtagcattt aatatgggga
    16921 gtcaactaca agagtttcaa caagcacaac atttggagtt agctgtcact ttatctgtta
    16981 atcaatggaa tggtgccaca accctacaat taatgttgga ggatgcgcgt gtagatggta
    17041 ttcaattatt tgatattaga agcaaagcaa gtagcttacc tcatggagtg ccaatcctgt
    17101 ctcaagaaga gcagagcaaa gaagttattc ttttgactgt tcccgatcat ccacaagaat
    17161 taaaacaaat gactcaaggt aaacagtttg atgctatcta ttttaaaaat gagataccaa
    17221 aaaattattt tatatctggt tatggtacac gtgatcaatt tgctagttta tataaaacaa
    17281 tatatcaatt cccagaattt gacgttcgtt ataagttaaa agagctcagt tcctaccttc
    17341 atattcctga tattttatta attaaaatga ttcaaatttt tgaagaacta cattttgtta
    17401 cgattacaga ggggattatg acagtcaata aagaagcaga aaagcgagat atctctgaga
    17461 gtcaaattta tcaagaatta aaagagacag ttaagtttca agaattaatg gctttaggga
    17521 cacctaaaga aatatat
//
LOCUS       DATRQA010000032        17395 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.032, whole genome shotgun sequence.
ACCESSION   DATRQA010000032 DATRQA010000000
VERSION     DATRQA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17395
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.032"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            221..1465
                     /locus_tag="VBD27_000768"
     CDS             221..1465
                     /locus_tag="VBD27_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO7024798.1"
                     /translation="MKIVEGVSLHLIKNQQFKTNHLTFRFSGDFNNKTVARRSLVAQM
                     LVTANAKYPKVQEFREKLASLYGASLSTKISTKGLVHIVDIDIVFVKNTFTLEQENIV
                     EQIITFLEDMLFSPLISLEQYQTSIFDTEKKNLIQYLEADIEDNFYSSDLALKSLFYN
                     NKTLRLPKYGTASLVESENSFTAYQEFQKMLKEDQLDIFVVGDFDDYRMIQAFNRMAF
                     EPRHKVLAFDYTQTYENITRSQVEDKDVNQSIMQLAYHLPITYKDEDYFALIVFNGLF
                     GAFAHSLLFTEIREKQGLAYTIGSQFDSFTGLFTIYAGIDKENRERFLKLINKQFNNI
                     KMGRFSSTLLKQTKDILKMNYVLASDNPKVIVDHIYHEHYLDQFHTSALFIDKVDDVT
                     KSDIVSVATKLKLQAFYFLEGN"
     gene            1467..2750
                     /locus_tag="VBD27_000769"
     CDS             1467..2750
                     /locus_tag="VBD27_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO7024799.1"
                     /translation="MTVNKITYQNLQEEVYKLTLESGLNVYLIPKPSFKETVGVLTAN
                     FGSLHTRYTRNGCVEHYPAGIAHFLEHKLFELDKGQDAATQFTKYGAESNAFTTFDKT
                     SFYFSTISHITNCLDILLDFVLTTNFTEESITKEKDIIKQEIEMYQDDPEYRLYQGVL
                     SNLYPNSPLAFDIAGDYQSISQITLTDLQENHKDFYQLSNMNLVLVGQFSPQEIITYL
                     QKNSHLTNYSQNIDRDSISLEPVIKNNSCHMTVTKPKLAIGYRKSSHMRHGSYLKEKI
                     GLQLFFAMLLGWTSTINQDWYESGQIDDSFDIEIEVHPDFECVIISLDTTEPIAFSTQ
                     LRLLLKNALQSSDLTESHLQNVKRELYGDFLRSLDSIENLAMQFVTYLYDGKTMYLDL
                     PSIVEELDLEDVITIGKDFLDNADTSDFVIFPKSS"
     gene            2873..3415
                     /gene="pgsA"
                     /locus_tag="VBD27_000770"
     CDS             2873..3415
                     /gene="pgsA"
                     /locus_tag="VBD27_000770"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO7024800.1"
                     /translation="MVKKENIPNLLTVVRILMIPLFIVLTSVTTSTTWHIVAAIVFAI
                     ASLTDYLDGYLARKWQVVTNFGKFADPLADKMLVMSAFIMLVGLDLAPAWVSAIIICR
                     ELAVTGLRLLLVETGGTVLAAAMPGKIKTATQMFAVIFLLVHWMTLGNIMLYIALFFT
                     LYSGYDYFKGAGFLFKDTFK"
     gene            3415..4254
                     /locus_tag="VBD27_000771"
     CDS             3415..4254
                     /locus_tag="VBD27_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO7024801.1"
                     /translation="MTNIITVNNLFFKYDSNQTHYQLENVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGQIIIDGQELTEDNVWELRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIPLKDMKERVDQALDLVGMSEFKMREPARLSGGQKQRVAIAGAVAMRPQ
                     VIILDEATSMLDPEGRLELIRTIRAIRQKYNLTVISITHDLDEVALSDRVIVMKNGKV
                     ESTSTPKALFGRGNRLISLGLDVPFTSRLMAELAANGLDIGTEYLTEKELEEQLWELN
                     LKM"
     gene            4230..5072
                     /locus_tag="VBD27_000772"
     CDS             4230..5072
                     /locus_tag="VBD27_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO7024802.1"
                     /translation="MGIEFKNVSYTYQAGTPFEGRALFDVNLKIEDVSYTAFIGHTGS
                     GKSTIMQLLNGLHIPTKGEVIVDDFSIKAGDKNKEIKFIRQKVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGISQIEAERLAEEKLRLVGISEDLFDKNPFELSGGQMRRVAIAGIL
                     AMEPKVLVLDEPTAGLDPKGRKELMTLFKNLHKKGMTIVLVTHLMDDVADYADYVYVL
                     EAGKVTLSGQPKQIFQEVELLESKQLGVPKITKFAQRLSHKGLNLPSLPITINEFVEA
                     IKHG"
     gene            5065..5859
                     /locus_tag="VBD27_000773"
     CDS             5065..5859
                     /locus_tag="VBD27_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO7024803.1"
                     /translation="MDKLILGRYIPGNSLIHKLDPRSKLLAMLLFIIIVFWANNVVTN
                     VIVFIFTLVIVGLSQIKFSYFFNGIKPMVGIILFTTLFQMLFAQGGQVIFSFWIFSIT
                     SLGLQQAALIFMRFVLIIFFSTLLTLTTTPLSLADAVESLLKPLEVLRVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIHKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGANRSKYRLLKWTVRDTFSILLMLLLGLSLFLLKN"
     gene            6075..6614
                     /locus_tag="VBD27_000774"
                     /pseudo
     CDS             6075..6614
                     /locus_tag="VBD27_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            6720..7424
                     /locus_tag="VBD27_000775"
     CDS             6720..7424
                     /locus_tag="VBD27_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO7024804.1"
                     /translation="MNKRRKLSKLNVKKQHLAYGAITLVALFSCILAVTVIFKSSQVT
                     TESLSKADKVRVAKKSKMTKATSKSKVEDVKQAPKPSQASNEAPKSSSQSTEANSQQQ
                     VTASEEAAVEQAVVTENTPATSQAQQTYAVTETTYKPAQHQTSGQVLSNGNTAGAVGS
                     AAAAQMAAATGVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQDQVN
                     SAIKAYRAQGLSAWGY"
     gene            complement(7559..8431)
                     /gene="sdaAA"
                     /locus_tag="VBD27_000776"
     CDS             complement(7559..8431)
                     /gene="sdaAA"
                     /locus_tag="VBD27_000776"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEO7024805.1"
                     /translation="MFYTIEELVEQANSQHKGNIAELMIQTEIEMTGRSREEIRYIMS
                     RNLEVMKASVIDGLTPSKSISGLTGGDAVKMDQYLQSGKTISDTTILAAVRNAMAVNE
                     LNAKMGLVCATPTAGSAGCLPAVISTAIEKLNLTEEEQLNFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSASAMAAAALVMAAGGTPFQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNALGSSFALVAADMALAGIESQIPVDEVIDAMYQVGSSLPTAFRETAEGGLAA
                     TPTGRRYSKEIFGE"
     gene            complement(8446..9114)
                     /gene="sdaAB"
                     /locus_tag="VBD27_000777"
     CDS             complement(8446..9114)
                     /gene="sdaAB"
                     /locus_tag="VBD27_000777"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEO7024806.1"
                     /translation="MKHLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGEPSE
                     VTFHLYNSFAKTYQGHGTDKALVAGILGMDTDNPDIKNSLEIAHQKGIKIYWDILKDS
                     NSPHPNTAKITVKNGDRSMSITGVSIGGGNIQVTELNGFSVSLTMNTPTLIIVHQDIP
                     GMIAKVTDILSDFNINIAQMNVTRESAGEKAIMIIEVDSRDCQQAVKKIEAIPHLHNV
                     NFFD"
     gene            9360..10481
                     /gene="mnmA"
                     /locus_tag="VBD27_000778"
     CDS             9360..10481
                     /gene="mnmA"
                     /locus_tag="VBD27_000778"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO7024807.1"
                     /translation="MTDNSNIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVTRDENGIVHMLRGADNNKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKDFLGQYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNKPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLTASEIHFTRDMPNEFKLECTAKFRYRQPDSKVTVYVKGNQARVVFD
                     DLQRAITPGQAVVFYNEQECLGGGMIDQAYRDDKICQYI"
     gene            10513..11124
                     /locus_tag="VBD27_000779"
     CDS             10513..11124
                     /locus_tag="VBD27_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEO7024808.1"
                     /translation="MISKFILAFMAFFAIMNPISNLPAFMALVADDDQKISRRIAAKG
                     VLLAFVIIVIFVLSGHLLFNLFGITLAALKISGGILVGIIGYKMINGIHSPTNKNLEE
                     HKDDPMNVAVSPLAMPLLAGPGTIATAMGLSSGGLSGKLITILAFAILCVIMYVILIS
                     ANEITKFLGKNAMTIITKMMGLILMTIGIEMLITGIKIGFHLT"
     gene            11294..13195
                     /gene="mnmG"
                     /locus_tag="VBD27_000780"
     CDS             11294..13195
                     /gene="mnmG"
                     /locus_tag="VBD27_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO7024809.1"
                     /translation="MTHNFAENYDIIVVGAGHAGVEASLAASRMGCKTLLATINLEML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KALYAQTMKQTVEKQENLTLRQAMIDEILVEDGKVVGVRTATNQKFSAKSVVITTGTA
                     LRGEIILGDLKYSSGPNNSLASVTLADNLRDLGLEIGRFKTGTPPRVKASSINYEKTE
                     IQPGDEQPNHFSFMSRDEDYITDQVPCWLTYTNTLSHDIINQNLHRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRYTEEVYVQGLSTSLPEDVQVDLLRSIKGL
                     ENAEMMRTGYAIEYDIVLPHQLRATLETKVIAGLFTAGQTNGTSGYEEAAGQGLVAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGYEIGLVDEERYAIFKKRQMQFENELERLDSIKLKPVSETNKRIQELGFKPLTD
                     ALTAKEFMRRPQITYAVATDFVGCADEPLDSKVIELLETEIKYEGYIKKALDQVAKMK
                     RMEEKRIPPHIDWDDIDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEG
                     RQKGRKNIN"
     gene            13262..15244
                     /locus_tag="VBD27_000781"
     CDS             13262..15244
                     /locus_tag="VBD27_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEO7024810.1"
                     /translation="MKRFRFATVHLVLIGLILFGLLAICVRLFQSYTALLLAIFVALS
                     FVVALLYYQKITYELSEVEQIELLNDQTEVSLKSLLEQMPVGVIQFDLETNDIEWFNP
                     YAELIFTGDNGHFQSATVKDIITSRRNGTAGQSFEYGDNKYSAYLDTETGVFYFFDNF
                     MGNRRNYDSSMLRPVIGIISIDNYDDIMDTMLEADMSKINAFVTSFISDFTQSKNIFY
                     RRVNMDRYYIFTDYSVLNTLIKDKFDILNEFRKRAQENHLSLTLSMGISYGDGNHNQI
                     GQIALENLNTALVRGGDQIVVRENDSSKKALYFGGGAVSTIKRSRTRTRAMMTAISDR
                     LKVVDSVFIVGHRKLDMDALGASVGMQFFASNIVNASYVVYDPNDMNSDVERAIDYLQ
                     EDGETRLVSVERAFELITQNSLLVMVDHSKTALTLSKEFFNKFADVIVVDHHRRDEDF
                     PKNAVLSFIESGASSASELVTELIQFQQAKDKLSRSQASILMAGIMLDTRNFASNVTS
                     RTFDVASYLRGLGSNSMAIQKISATDFDEYRLINELILKGERIYDNIIVATGEEHKVY
                     SHVIASKAADTMLTMAGIEATFVITKNSSNIGISARSRNNINVQRIMEKLGGGGHFSF
                     AACQIQDKSVKQVRRMLLEIIDEDLRENSTVENRRD"
     gene            15250..15702
                     /gene="rplI"
                     /locus_tag="VBD27_000782"
     CDS             15250..15702
                     /gene="rplI"
                     /locus_tag="VBD27_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO7024811.1"
                     /translation="MKVIFLQDVKGKGKKGEVKEVPTGYAQNFLLKKNLAKEATTQAI
                     GELKGKQKSEEKAQAEILAQAKELKTQLESETTRVQFIEKVGPDGRTFGSITAKKIAE
                     ELQKQYGIKIDKRHIDLDHTIRAIGKVEVPVKLHKQVSSQIKLDIKEA"
     gene            15745..17100
                     /gene="dnaB"
                     /locus_tag="VBD27_000783"
     CDS             15745..17100
                     /gene="dnaB"
                     /locus_tag="VBD27_000783"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO7024812.1"
                     /translation="MVEVSELRVQPQDLLAEQAVLGSIFISPEKLIMVREFISPDDFY
                     KYSHKVIFRAMITLADRNDAIDAATVRNILDDQGDLQNIGGLGYIVELVNSVPTSANA
                     EFYAKIVSEKAMLRDIISKLTDTVNMAYEGNDSDEIIATAEKALVDINEHSNRSGFRK
                     ISDVLKVNYENLELRSQQTSDVTGLPTGFRDLDRITTGLHPDQLIILAARPAVGKTAF
                     VLNIAQNVGTKQNRPVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVT
                     IAQGALADAPIYIDDTPGIKITEIRARSRKLSQEVDDGLGLIVIDYLQLISGTRPENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYRREGEEAEEIVEDNTVEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFE
                     E"
     gene            17112..>17395
                     /locus_tag="VBD27_000784"
     CDS             17112..>17395
                     /locus_tag="VBD27_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEO7024813.1"
                     /translation="MSDAFADVAKMKKIKEDIKSHEGQMVELTLENGRKREKNKIGRL
                     IEVYPSLFIVEYKDTAAVPGAIDNTYVESYTYSDILTEKTLIRYFDDESA"
BASE COUNT         5740 a         2713 c         3388 g         5554 t
ORIGIN      
        1 tttagatatc acatcattat agcataaaat tattttttga gttgaactta tgatatggag
       61 acaaggtata aaaaatggga gtaaatattg gttgacgact ttgtcaattt tgaagaagat
      121 tgaaatttag aattaatcat atcttggatg tttaatcttt tttaacgcct ttaaaaatga
      181 tataatctta gctaacaata cagaacacaa aggatattga atgaaaatag ttgaaggcgt
      241 ttctctacat ttaattaaaa accaacaatt taagactaat catcttactt ttcgattttc
      301 aggtgatttt aataataaaa cagtagcacg tagatcgctc gttgctcaaa tgttagttac
      361 tgcaaatgct aagtacccta aggtacaaga atttcgagaa aagttagctt ccttatatgg
      421 tgctagttta tcaactaaga tttctactaa gggtcttgtt cacattgttg atattgatat
      481 tgtatttgtt aaaaatacct ttactttaga acaagaaaat atcgttgaac aaattattac
      541 attcttggag gatatgctgt tttctccttt aatttcttta gaacagtatc aaacatctat
      601 ttttgatact gagaagaaaa accttattca atatttagag gccgatattg aagataattt
      661 ttatagcagt gacttagcac taaaatcttt attctacaat aataagacac ttcgtttacc
      721 taagtatggt acagcatctt tagtggaatc agaaaattca tttacagctt atcaagaatt
      781 tcagaagatg cttaaggaag atcaattaga tatttttgtc gtaggagatt ttgatgacta
      841 tcgaatgatt caggcattta atcgtatggc atttgagcca cgtcacaaag tattagcttt
      901 tgattatacg cagacttatg aaaatatcac gagaagtcaa gtagaagata aggacgttaa
      961 tcaatctatt atgcaattgg cttatcatct tccaataact tataaagatg aagattattt
     1021 tgctttaata gtatttaacg gtttatttgg tgcttttgcc cattccttgt tgtttacaga
     1081 aattcgagaa aagcaaggtt tagcctatac tataggtagt caatttgaca gctttacagg
     1141 tctctttacg atatatgcag gtattgataa agagaatcgt gagcgttttt tgaagcttat
     1201 taataagcaa ttcaataata ttaagatggg caggttctct tctacattat tgaaacaaac
     1261 aaaagatata ttgaaaatga actatgtttt agcttctgat aatcctaagg tcatagttga
     1321 ccacatttat catgaacatt atttagatca gtttcatact tcagcactat tcattgacaa
     1381 agtggacgat gtaacaaaat cagatattgt cagcgtagca acaaaattaa aattacaagc
     1441 tttttatttt ttggaaggaa actaggatga cagttaataa aataacttat cagaaccttc
     1501 aggaggaagt atataagtta acattagaga gcgggctaaa tgtatatctt attccaaaac
     1561 catcatttaa ggaaactgtt ggagtattga cagcaaattt tggatcactc catacaagat
     1621 atacgaggaa tggttgtgta gaacattatc cggcaggaat tgctcacttc ttagaacata
     1681 aattatttga actggataaa ggacaagacg ctgcaactca atttactaaa tatggcgccg
     1741 agagtaatgc ctttacaact tttgataaaa ctagttttta tttttcaact ataagtcaca
     1801 ttacaaactg cttagatata ctacttgact ttgtattgac aacaaacttt acagaagagt
     1861 caattactaa agaaaaagac attatcaaac aagaaattga aatgtatcaa gatgacccag
     1921 aatataggct ttatcaagga gtgttatcga acctttaccc caactctcca ttagcatttg
     1981 atatcgctgg agattaccaa tcaatctctc aaataacctt aaccgattta caagaaaatc
     2041 acaaagactt ttatcagttg tctaatatga atttagttct tgttggacaa tttagtccac
     2101 aagaaattat aacttacctt caaaaaaatt ctcatttaac aaattactca cagaatattg
     2161 accgagattc tattagtctt gaacctgtta taaaaaacaa ctcttgccat atgacagtga
     2221 caaaacctaa attagctatt ggttatcgaa aatcaagtca tatgagacat ggttcttacc
     2281 taaaagagaa aatcggatta cagctatttt ttgcaatgct tttaggatgg acttctacaa
     2341 ttaatcaaga ctggtacgaa tctggtcaaa ttgatgactc ttttgatata gaaattgaag
     2401 ttcatccaga ttttgagtgc gttattattt ctttagatac gacagagcca attgcttttt
     2461 caactcagtt gaggttattg ctaaaaaatg ctttgcaatc atctgatctt accgaaagcc
     2521 acttacaaaa cgttaagaga gagctctatg gcgatttttt aaggagctta gattctattg
     2581 aaaatctcgc aatgcaattc gtcacctatt tatatgatgg aaaaacgatg tatttagatt
     2641 tgccatctat cgttgaagaa ttagacttgg aagatgtcat tacgattggt aaggatttct
     2701 tagataatgc tgacacatct gacttcgtta tttttccaaa atcttcgtaa ataatctgaa
     2761 attttgatat aataaggaag cttagacatc tataaagttg gtatgtcaaa agtcttttac
     2821 tagttttgaa ataacataat ataggaatac tatgtttcca aaaggagaat ttatggtgaa
     2881 gaaagaaaat attccgaatc tattaactgt agtcagaatt ttaatgattc cgttatttat
     2941 agtattaact tcagtaacaa caagtacaac ttggcatatt gttgcagcta ttgtatttgc
     3001 tattgcaagc ttgaccgatt acctagacgg ttatctagca cgtaaatggc aagtagtaac
     3061 aaattttggg aaatttgcag atccattagc tgataaaatg ctagttatga gtgcttttat
     3121 catgttagtt ggactagact tagcaccggc ttgggtttca gctattatca tttgtagaga
     3181 gctagcagtt acaggtttgc gtttactttt agttgaaaca ggtggaacag tacttgctgc
     3241 agctatgcca gggaaaataa agacggctac tcaaatgttt gcagttattt ttttgttagt
     3301 acattggatg acacttggta acatcatgtt atacattgct ttattcttta ccttgtattc
     3361 aggctacgac tattttaaag gtgcaggctt cttatttaag gatactttta aataatgaca
     3421 aatattatta ctgttaataa tctctttttt aaatatgata gtaatcaaac acattatcaa
     3481 ttggagaatg tatcgtttca tgtgaaacaa ggagaatggc tatctattat cggtcataat
     3541 ggttctggga aatccactac tgttcgttta atagatgggc ttttagaggc ggagtcaggc
     3601 cagattatca ttgatggaca agagttaacc gaagataatg tctgggagtt acgtcacaag
     3661 attggcatgg tatttcaaaa tcctgataac caatttgtag gggcaactgt tgaagatgac
     3721 gtcgcctttg gtttggaaaa taaaggtatt cctttgaaag acatgaaaga aagagtggac
     3781 caagcacttg atttggtagg catgtctgaa tttaaaatga gggagcctgc acgtttatct
     3841 gggggacaaa agcagcgtgt agccattgca ggagctgtag ctatgcgtcc acaagttatc
     3901 atcttggatg aggcgactag tatgcttgat cctgagggac gattggaact gataagaacg
     3961 atacgagcta ttcgtcaaaa atataatctt actgttattt caattacaca cgacttagat
     4021 gaagttgcat tgagtgatcg tgtcatcgtt atgaaaaatg gtaaagttga gtcgacatcc
     4081 actccaaaag cgttatttgg tcgtggaaat cgtttgatta gtttaggttt agatgttccc
     4141 tttacaagta ggttaatggc agaactggct gctaatggtc ttgatatagg gacagagtat
     4201 cttacagaga aggaattaga agaacaatta tgggaattga atttaaaaat gtaagttata
     4261 cctatcaagc cggcactcct tttgaagggc gtgccctttt tgacgtcaat ctgaaaattg
     4321 aagatgtttc ctataccgcg ttcattgggc acacaggttc tggaaaatca actattatgc
     4381 aacttttgaa tggtttacat attcctacaa aaggtgaggt aattgtcgat gatttttcta
     4441 ttaaagcagg ggacaagaac aaagaaatca aatttataag gcaaaaagtt ggtttagttt
     4501 ttcaatttcc agaaagtcag ctttttgaag agacagtttt aaaggatgtt gcttttggac
     4561 cacaaaattt tggtatttct cagattgaag ctgaaaggct ggctgaagaa aaattaaggt
     4621 tagttggtat cagtgaggat ttattcgata aaaatccatt tgaactttct ggagggcaga
     4681 tgaggcgggt tgctatagct ggtattttag cgatggaacc caaagtacta gtactggatg
     4741 agccaacagc tggacttgat cctaagggaa gaaaagaatt aatgactctt tttaaaaatc
     4801 ttcataaaaa aggaatgact atcgtcttag tgactcactt aatggacgat gtagcggatt
     4861 atgctgacta tgtgtatgtt ttagaagcag ggaaagtaac cttatcagga caaccaaaac
     4921 agatttttca agaagtagaa cttttagaaa gtaaacaatt aggagttccc aaaatcacca
     4981 agtttgctca aagactatct cataagggat taaatttacc tagtttacca attactatta
     5041 acgaatttgt ggaggctatt aagcatggat aaattgattt tgggacgtta cataccaggt
     5101 aattctctca ttcataaatt agatcctcga agtaagttac tagccatgtt gctttttatc
     5161 attattgttt tttgggcaaa taatgtagtg acaaatgtga tcgtattcat ttttacgttg
     5221 gttattgtgg gtttatctca gataaaattt tcctattttt tcaatggtat taaaccgatg
     5281 gttggtatta ttttatttac aaccctgttc cagatgctgt ttgcacaagg cgggcaggtt
     5341 atcttctcgt tttggatttt tagcatcact agccttgggt tacaacaagc agcacttatt
     5401 tttatgagat ttgttttaat tatcttcttt tcgacattac ttaccttaac tactactccg
     5461 ttaagtttag cagatgctgt tgaatctttg ttaaaaccat tggaagtact gagagtacca
     5521 gcacacgaga ttggtttgat gttatccctt agcttacgtt tcgttcctac tttaatggat
     5581 gatacaacac gtatcatgaa tgctcaaaga gctcgtggag ttgattttgg agagggaaac
     5641 ctaattcata aggttaagtc cattatccct attttgattc cattatttgc ttctagtttt
     5701 aaaagagcag atgcgttggc aattgctatg gaagcaagag gttatcaagg tggagccaat
     5761 cgtagtaagt accgtttatt gaagtggact gttcgggata cttttagtat attactgatg
     5821 ttattattgg gtttgagttt gtttctatta aaaaattaaa aggttaaggt cagtttatgg
     5881 tcttagcctt ttcatttata taatgtgaaa agctcaacaa ctttacttaa agaaaactaa
     5941 aaattgtttt ttggaggcct tttctttatt aatttacaat ttatttgtaa taaatttcat
     6001 ctaaggcaaa acaattagta atatttacaa ggtatgatag tacgcgtatc aattaatgaa
     6061 agagagcata tattatgtca ataacctcgg ttaaaaaatc aaaaccattt aaattaggag
     6121 tggcaggtct tttagtgggt gcttcattag ctttaccact ttcagtaagc gcagcatctt
     6181 ataccgtgaa atcaggtgat accttatcag ctattgctaa aaatcataaa actacggtac
     6241 aatagttagt gtctctcaat agtatcagta acgctgatgt catcagtata ggtgatgttt
     6301 taaaattgga taattctaca gctagtcaag cagaagcaaa atctcaacca acaattgaaa
     6361 attcaatgaa ttcttcatca aatttgagtt caagtgattc agctgcaaaa gaagaaatag
     6421 ctcgtcgtga atcaaatggt agttatactg cacagaatgg acaatattat ggaagatatc
     6481 aactgtctca atcttaccta aatggcgact tatctcctga aaatcaagaa aaagtagcgg
     6541 acaattatgt ggcttctcgt tacggatctt ggtcggcagc gctatcattt tggaatagta
     6601 acggttggta ttaataaaag ttgataaaac aaagagattt acttaaaatt aaatctcttt
     6661 gtaatatttt taacatctat tcaattgcaa ggtaacaaat aaagtataat ataggtaaca
     6721 tgaataaaag aagaaaatta tcaaaattga atgtaaaaaa acaacattta gcttatggag
     6781 ctatcacttt agtagccctt ttttcatgta ttttggctgt aacggtcatc tttaaaagtt
     6841 cacaagttac tactgaatct ttgtcaaaag cagataaagt tcgcgtagcc aaaaaatcaa
     6901 aaatgactaa ggcgacatct aaatcaaaag tagaagatgt aaaacaggct ccaaaacctt
     6961 ctcaggcatc taatgaagcc ccaaaatcaa gttctcaatc tacagaagct aattctcagc
     7021 aacaagttac tgcgagtgaa gaggcggctg tagaacaagc agttgtaaca gaaaataccc
     7081 ctgctaccag tcaggcacaa caaacttatg ctgttactga gacaacttac aaacctgctc
     7141 aacaccagac aagtggccaa gtattgagca atggaaatac tgcaggggcg gtcggatctg
     7201 ctgctgcagc acaaatggct gctgcaacag gagtccctca gtctacttgg gaacatatta
     7261 ttgcccgtga atcaaatggt aatcctaatg ttgctaatgc ctcaggagct tcaggacttt
     7321 tccaaacgat gccaggttgg ggttcaacag ctacagttca ggatcaagtt aattcagcta
     7381 ttaaagctta tcgtgctcaa ggtttatcag cttggggtta ctagtgaata gctataaatt
     7441 aactttacac tattgtcaat aataattaca gattgctaaa acaaataaat aaaaacgcta
     7501 cagtaaagct gtagcgtttt tatttatttt cagggaaaga gtagaaaatt gataaacttt
     7561 attccccaaa aatttctttg ctataacgtc ttcctgtcgg cgtggcagca agtcctcctt
     7621 ctgcagtttc acgaaaagca gtcggtaaac ttgatccaac ttgatacatt gcatcaataa
     7681 cttcatctac tggaatttgc gattcaatac cagccaaggc catatcagca gcaacaagtg
     7741 caaaacttga tccaagagca ttccgcttca cacaagggac ttcaactaaa cctgcaacag
     7801 ggtcacagat aagtccaagc atatttttaa taacaaatgc tatagcttgg ctagcttgga
     7861 aaggagtacc tccagcagcc ataactaaag cagccgcagc catagcacta gctgacccaa
     7921 cttcagcttg gcaacctcct tctgcacctg agatagaggc attattacca atgacaagac
     7981 caaatgcgcc ggctgtaaat agaaaattaa gttgctcttc ttctgttaaa ttaagctttt
     8041 caatggctgt agaaatcaca gctggtaaac atcctgcact acctgcagtt ggtgttgcac
     8101 agaccagtcc catcttagca tttaactcat taacagccat agcattccta acggcagcta
     8161 ggattgtggt atctgaaata gtttttcctg attgtaaata ttgatccatc ttgacagcat
     8221 caccgcctgt taaaccactg attgatttac taggggtcaa tccatcaata acagaagctt
     8281 tcataacctc aaggtttcgg gacataatat aacgaatttc ttcacgactt ctaccagtca
     8341 tttcaatttc cgtttggatc atgagctctg ctatgttacc cttatgttgg ctattagctt
     8401 gctctaccag ctcttcaatt gtataaaaca tagttaccct ttctcttaat caaagaagtt
     8461 gacattgtgt aaatgaggaa tagcttctat ctttttgaca gcttgttgac aatctcgtga
     8521 atctacttct ataatcatga tagctttttc tcctgcactt tcgcgtgtta cattcatttg
     8581 agcaatatta atgttaaagt cagataaaat atcagttact ttagcaatca tgccaggtat
     8641 atcttgatga acgataatta aagtaggagt attcattgtt aatgaaactg agaatccatt
     8701 caactcagta acttggatgt ttccgccacc aattgacact cctgtaatac tcatgctcct
     8761 atccccattt ttaacagtta ttttcgcagt atttggatgt ggtgaattac tatcttttag
     8821 gatatcccag taaatcttaa ttcctttttg atgagcaatt tcaagagagt ttttaatatc
     8881 tggattatct gtatccattc ctagaatccc tgcaaccaat gctttatcag taccgtgtcc
     8941 ttggtaagtt ttagcaaaag aattgtataa atgaaaggtt acttcactag gttcaccaaa
     9001 aatagaatgg acaactttac caatgcggac agctcctgca gtatgactac ttgatggtcc
     9061 aatcataaca ggaccaataa tgtcgaagac cgattgaaat tttaaatgtt tcatagtttt
     9121 tcctcgtgat ttttgtacat tctcattata acagattatt tctaaaaaat atcataaaaa
     9181 gataaaaaaa ctgcaatgta ataaatattt atcacaacaa aaacatacaa cttgacaaca
     9241 aatagtgcta taatatataa gcacttttat agtgtttttt agtcaatatg attgatctaa
     9301 gaacaacttg gcccttcaag cctaagttaa aaataaaact ttttgatggg agaattctta
     9361 tgactgataa ttcaaacatt cgtgtcgttg taggcatgag tggaggtgtt gattcatcag
     9421 taactgccct gcttttaaaa gagcaaggtt atgatgttat tggtgttttt atgaaaaatt
     9481 gggatgacac agatgaattt ggtgtttgta cggctactga agattataaa gatgttgctg
     9541 ctgttgctga tcagatcggc ataccatact actctgtcaa ctttgaaaaa gagtattggg
     9601 acagggtttt cgaatatttt ttagcagaat atcgagcagg tcgtacgcca aatccagatg
     9661 ttatgtgtaa taaggaaatt aaatttaaag catttctaga ttatgcgatg acattaggtg
     9721 ctgactatgt agcaacaggt cattatgcac aagtaacacg tgatgagaac ggaatagttc
     9781 atatgcttag aggagcagat aataataagg atcaaactta ttttctaagt caattatctc
     9841 aggagcaatt gcaaaaaacg ttatttccac ttggtcattt acaaaaacca gaagttcgcc
     9901 gtatagctga agaagcagga cttgcgaccg ctaagaaaaa agactctact ggtatttgtt
     9961 ttattggaga aaaaaatttc aaggatttcc tgggtcaata tttaccggct caaccaggtc
    10021 gtatgatgac tgttgatggt cgtgacatgg gtgaacatgc tggtttaatg tattatacta
    10081 ttggtcaaag agggggcctt ggtattggtg ggcaacatgg tggagataat aaaccttggt
    10141 ttgtagttgg aaaggattta agtaaaaaca ttctttatgt aggtcaaggt ttctatcatg
    10201 actcattgat gtcaactagc ttgacagctt ctgaaatcca ctttacacgt gatatgccta
    10261 atgaatttaa gctagaatgt acagctaagt tcagatatcg tcaacctgac agtaaagtta
    10321 cagtttacgt aaaaggtaat caagcaaggg ttgtgtttga tgatttacaa cgagcaatca
    10381 caccaggtca agctgttgtt ttttataatg aacaagaatg tttaggcggt gggatgattg
    10441 atcaggctta tcgtgatgat aaaatctgtc aatacattta attgacactt aattattaag
    10501 tgaggtaatc gtatgatttc aaaatttatt ttagctttca tggctttttt cgccattatg
    10561 aatcccattt ctaatttgcc agcttttatg gctttagtag ctgatgatga tcagaagatt
    10621 tcacgtcgga ttgcagcaaa aggagtatta cttgcttttg tcattattgt aatctttgta
    10681 ttaagtggcc acttactttt taacttattt gggattaccc tagccgccct aaaaatatct
    10741 ggtggaatat tggtaggcat tataggttat aaaatgatta atggtataca ttcaccaacc
    10801 aataaaaacc tagaggagca taaggatgat ccaatgaatg tggcagtttc accattagca
    10861 atgccccttt tggcaggtcc gggtactatt gcaactgcta tggggttatc aagtggtggt
    10921 ttatctggca aactcattac tatacttgct tttgccatat tatgtgtgat aatgtatgtt
    10981 attttaataa gtgcaaacga aattactaaa tttcttggaa aaaatgcaat gactataatc
    11041 accaaaatga tgggacttat tttgatgact atcgggatag agatgctaat aacaggcata
    11101 aaaattggtt ttcatttaac atagcctatc ccctctggtt gacttttaaa agataaatgc
    11161 taaaataaat ttaacaataa ctatgatggt caaagctcat ggaatttgta agtctttttg
    11221 acgtacaatt ttcgagagtt ttggctattt ttgtgtttta atgaattaga ctttgaaatt
    11281 ggaaaggaat aaaatgacac ataattttgc agaaaactat gatattatcg ttgttggagc
    11341 gggtcatgca ggtgtggaag ctagtctagc tgctagtcgt atgggatgta agacacttct
    11401 agccactatc aacttagaaa tgttagcatt tatgccttgt aatccttcta taggtggctc
    11461 tgctaaaggt atcgttgtca gagagattga cgctttaggt ggtgaaatgg gtaaaaacat
    11521 tgataaaacc tatatacaaa tgaaaatgct taatacaggt aaggggcctg ctgtccgtgc
    11581 cttaagagca caagctgata aagccctcta tgcacaaaca atgaagcaaa ctgtagaaaa
    11641 gcaagaaaat cttaccttaa gacaagctat gattgacgaa atacttgttg aagatggaaa
    11701 ggttgtgggg gttcgtacag caactaatca gaaattttca gcaaaatctg ttgttataac
    11761 aacaggtact gctcttcgag gagagattat attaggtgat cttaagtact cctcgggccc
    11821 taataatagt ttagcttctg tgacattggc agataattta agagacctag gattagaaat
    11881 tggtcgtttc aaaactggaa cacctcctcg tgtaaaagcc tcctctatta attatgaaaa
    11941 gacagagata caacctgggg atgagcaacc aaatcatttt tcatttatgt ctagggatga
    12001 agattatatt actgaccagg taccgtgctg gttgacctat acgaatactt tgagtcatga
    12061 catcatcaat caaaatctac accgtgctcc tatgttttct ggaatcgtta agggagttgg
    12121 tcctagatat tgtccgtcaa ttgaagataa aattgtgcgt tttgctgata aagagcgcca
    12181 ccaactcttc ttagaaccag aaggtagata cactgaagaa gtttatgttc aagggctatc
    12241 aacgagtctt cctgaggatg ttcaagttga tttattacgt tctataaaag ggcttgaaaa
    12301 tgctgaaatg atgcgtacag gctatgctat tgagtatgat attgtattgc ctcatcaatt
    12361 aagagcaaca ctcgaaacaa aagtaattgc tggtttgttt actgcaggac agactaatgg
    12421 tacttcaggt tatgaagaag ctgctggtca agggttggtt gctgggatca acgctgctct
    12481 taaagttcaa gggaagccag aacttatttt aaaacgaagt gatgcttaca ttggtgtcat
    12541 gattgatgat ttagttacca aagggactct agaaccatat cgtctgttga caagtcgcgc
    12601 agaatatcgt ctgattcttc gtcatgataa tgcagatatg cgtttaacag aaatcggtta
    12661 tgagattggc ctagtagatg aagagcgtta tgcgatattt aaaaagcgtc aaatgcaatt
    12721 tgaaaatgaa ttagaacgtt tagactctat aaagttaaag ccagtttctg agacaaataa
    12781 acggattcaa gaattgggct ttaaaccttt gacagatgct ttaactgcca aagaatttat
    12841 gcgccgccca caaatcacat atgcagtagc aacggatttt gttggttgtg ctgatgagcc
    12901 attagacagt aaggttattg aacttttgga aactgaaatt aaatacgaag gctatattaa
    12961 aaaggcttta gatcaagttg caaaaatgaa acgaatggaa gaaaaacgta ttccccctca
    13021 tattgactgg gacgacattg attctatcgc aacagaagct agacaaaaat tcaaaaaaat
    13081 taacccagaa acgctaggtc aggctagtcg tatttcagga gttaatccag ccgatattag
    13141 tattttaatg gtttaccttg aaggaagaca aaaaggtcgt aaaaatatca attgataata
    13201 gtgttcgccc ctgtttatca ggggcgtttt gtgttataat taggtcttaa gaggttattt
    13261 aatgaaaaga tttcgttttg caaccgtcca tttagtttta ataggcctga ttttatttgg
    13321 actattagct atttgtgtta ggttatttca atcctatacg gctctattat tggctatttt
    13381 tgttgcttta tcttttgttg ttgccttatt atattaccaa aagataactt atgaactatc
    13441 tgaagttgaa caaattgaat tacttaatga tcaaacagaa gtttctctta agagtcttct
    13501 tgaacaaatg ccagttggtg ttatacaatt tgatcttgaa acaaatgata ttgagtggtt
    13561 taacccttat gcagaattga tttttactgg ggataatggt cattttcagt ctgctactgt
    13621 taaagatatc attactagta gacgtaatgg aacggctggg caatcatttg aatacggtga
    13681 taacaaatat tcagcttatt tggatacaga gacgggtgtt ttctatttct tcgataattt
    13741 catggggaat agaagaaact atgatagttc aatgctacgt ccagttattg gtattatttc
    13801 cattgataac tacgatgata ttatggatac tatgttagag gctgatatgt caaagattaa
    13861 cgccttcgtg actagtttta tttctgattt tacacagtct aaaaatattt tttatcgtcg
    13921 tgtcaatatg gatcgatact atatttttac cgactatagc gttttaaata cattgataaa
    13981 agataaattt gatattttaa atgagtttcg aaaacgcgct caggaaaacc atttatcatt
    14041 aacattaagt atgggaattt cttatggtga tggtaaccat aatcaaattg ggcaaattgc
    14101 tttagaaaat cttaatacag cactcgttcg cggaggagat caaatagtag tacgtgaaaa
    14161 tgatagtagt aaaaaagcac tttactttgg tggtggtgct gtttcaacta ttaaacgttc
    14221 acgaacacgt accagagcta tgatgactgc tatttctgac cgtttgaaag tagttgatag
    14281 tgtctttatt gtgggacaca ggaaattgga catggatgca ctaggtgcat ctgtagggat
    14341 gcaatttttt gctagtaata ttgttaatgc cagttatgta gtttatgacc caaatgacat
    14401 gaattctgat gttgaaagag caattgatta cttgcaagaa gacggcgaaa cacgtctcgt
    14461 aagtgttgag agagcttttg aattaattac tcaaaattct ttattagtca tggtagacca
    14521 ttctaaaaca gctttaacct tatctaagga attctttaac aagtttgcag atgttattgt
    14581 ggtagatcat catagaagag atgaagattt tccaaaaaat gctgttcttt catttataga
    14641 aagtggcgca agtagtgcca gcgaattagt tacagaattg attcaatttc aacaagctaa
    14701 agataaatta agccgcagtc aagcaagtat tttaatggct ggtatcatgc ttgatacaag
    14761 aaattttgca tctaatgtaa cgagtagaac ttttgatgtg gcaagctatt tacgcggttt
    14821 aggtagtaat agtatggcta ttcaaaaaat atcagctaca gattttgatg aatatcgttt
    14881 aattaatgaa ctgattttaa aaggtgagcg tatttatgat aacatcattg ttgctaccgg
    14941 agaagaacat aaagtttact cgcatgttat agctagtaaa gcagctgaca ccatgttgac
    15001 aatggcaggt attgaggcta cttttgttat tactaaaaac agttcaaata ttggtatttc
    15061 agcgcgcagt agaaataata tcaatgtcca gcgtattatg gaaaaacttg gtggtggcgg
    15121 acattttagt tttgctgctt gtcaaataca agacaagtct gtaaagcaag ttagacgtat
    15181 gttactggaa ataattgacg aagatttaag agaaaatagt acagtcgaaa atagaaggga
    15241 ttaattgaga tgaaagttat ttttttacaa gatgttaagg ggaaaggaaa aaaaggtgag
    15301 gttaaggaag tacctactgg ttatgctcaa aacttcttat taaaaaagaa tctagctaaa
    15361 gaagcaacga cacaagctat cggtgaatta aaaggaaaac aaaagtccga agaaaaagca
    15421 caagcagaaa ttttagcaca ggcaaaggag ctgaaaacac aattagaatc cgaaactacc
    15481 cgagtgcaat ttattgaaaa ggtaggtcca gatggtcgta cgtttggttc tatcacagcc
    15541 aaaaagattg ctgaagagtt acaaaaacaa tacggcatta aaattgacaa acgtcatatt
    15601 gatttagatc atactattag agcgattggt aaggttgaag taccagtaaa attacataaa
    15661 caggtttctt cacagattaa gcttgatatc aaagaagcgt aaactgagag atgataaagg
    15721 ttgacaagtc aggaggaatg aagtttggta gaagtatctg agctaagagt acaacctcaa
    15781 gacctgttgg cagaacaggc cgttctagga tctatcttta tctcaccaga gaaactgatt
    15841 atggtgagag aatttataag tccagatgat ttttataaat attcacacaa agttatcttc
    15901 agagcgatga ttaccttagc tgatagaaat gatgcaattg atgctgctac tgttagaaat
    15961 attttagatg accaaggtga tcttcaaaac attggtgggt tgggctatat tgttgaatta
    16021 gttaatagtg ttccaacaag tgctaatgcg gagttttatg ccaaaatagt ttctgagaaa
    16081 gccatgttgc gagatatcat ttctaagttg acagatactg tcaacatggc ctatgaagga
    16141 aatgattctg atgaaattat tgccaccgct gagaaggctt tagttgatat taacgaacac
    16201 agtaatcgta gcgggtttag aaagatatct gatgttttaa aagttaatta cgaaaactta
    16261 gaattacgtt cacagcaaac ctcagatgtc acaggtttac caactggatt tagagattta
    16321 gacaggatta ctacaggttt acatccagat caattaatca tcttagcggc tcgtcctgca
    16381 gtaggtaaaa cagcctttgt tttaaatatt gctcaaaatg ttggaactaa acaaaataga
    16441 cctgtagcta ttttttcact tgaaatgggg gctgagagtt tagttgaccg tatgttggca
    16501 gcagagggaa tggttgattc tcatagttta cgaactggtc agttgacgga tcaagattgg
    16561 aataatgtaa cgattgctca aggagctttg gcggatgcgc caatttatat tgatgatacg
    16621 cccgggatta aaattacaga aattagagca cgctctagga agttatctca agaagttgat
    16681 gatggtttag gtttaatcgt cattgactac ctccaattaa tttcaggaac tagaccagaa
    16741 aatcgtcagc aagaagtttc tgaaatttca aggcagttga agattttagc taaagaatta
    16801 aaggttcctg ttatcgcatt aagccagctc tctcgtgggg ttgagcaaag gcaggataaa
    16861 cgaccggtct tatctgatat tcgtgaatct ggatcgattg aacaagatgc tgacattgtt
    16921 gctttcttat atcgagatga ttattatcgt agagagggcg aagaagcaga ggaaattgta
    16981 gaagataata ctgttgaggt tattttagaa aagaaccgtg ctggtgcgcg tgggacagta
    17041 aaattaatgt ttcaaaagga atataataaa ttttctagta tagctcaatt tgaagaatag
    17101 gagatgttaa tatgagtgat gcatttgcag atgttgcaaa aatgaaaaaa ataaaagaag
    17161 atatcaagtc acatgaggga cagatggtcg aacttactct cgaaaatggt cgtaagaggg
    17221 aaaaaaataa aattgggcgc ctaattgaag tttacccatc tttgtttatc gtagagtata
    17281 aggatactgc agctgtacca ggagcaattg ataatactta tgtagagtct tacacttatt
    17341 cagatatttt aactgagaaa actttaattc gttattttga tgacgagtca gcaga
//
LOCUS       DATRQA010000033        17265 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.033, whole genome shotgun sequence.
ACCESSION   DATRQA010000033 DATRQA010000000
VERSION     DATRQA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.033"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            79..2115
                     /locus_tag="VBD27_000785"
     CDS             79..2115
                     /locus_tag="VBD27_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO7024814.1"
                     /translation="MVDNKTVVIMLVFLARKNLSLYELTVQTKFSIKVIIEQINYLNS
                     FLAKNHLPAIAHSAGRYQLLGDEKEHDKIVSLLEAEQFYLTQEERVCLIYLYSFCRRE
                     FVSNVHYQDFLKVSKNTTLSDIKMLRSKLAKRGISLTYTRAKGYSLVGDEMDKHQVAF
                     QMITQLLESPIGFWSLNYILSSWKFALSYEKLEKTVEYFYESFQLSPIQDRLEKSLYF
                     IILILCRYQRSVDRVLQGSPIVSEQLKELTTIIVTNLSQDISLSKPLDQKEKDYITLI
                     LSGCFEGEGTKDDDFFEALAKAIVDEMETVSLLNFSNKEELLQGLKRHIIPAYFRLKY
                     GLTGDSGYTQNIKEHYSDLFLLVKKALRPLEEQVGLIPDSEISYFVIHFGGYLRQSGD
                     TQSMSYKALILCPNGVSSSLVIKEKLRGLFPQIHFHRVSKIEQLKLIDNQTYDMVFST
                     IFVETKKPNYLVSLMMTAEQVQQLKELVISDFPKACLDDFQLDQLIATIKKYAHVHCE
                     EELKLALRTMVKQDIFRKDVRPLLHQLITEETYQTSSEQMNWKEAIRLAAKPLLASGK
                     ITESYPEAMIEKVEEFGPFINLGKGIAIPHARPEDGVNSVGMSMLVLEQPIYLLDDPK
                     QEIYLLLCIAAIDNETHLKALSHLTTILRDNNNVKALLASRGYQDIEMIIKQED"
     gene            2118..2402
                     /locus_tag="VBD27_000786"
     CDS             2118..2402
                     /locus_tag="VBD27_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7024815.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILKDLGVSDVEVEHYDLGGA
                     DPSAADVWIVGRDLEDSAGHLGDVRILNSIIDMDELRELVTGICQEKGLI"
     gene            2415..3770
                     /locus_tag="VBD27_000787"
     CDS             2415..3770
                     /locus_tag="VBD27_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO7024816.1"
                     /translation="MKGLLDFLVNIARTPAILVALIAIIGLVLQKKGVPDIVKGGIKT
                     FVGFLVVSGGAGIVQNSLNPFGKMFEHAFHLVGVVPNNEAIVAVALTKYGSATALIML
                     AGMIFNILIARFTKFKYIFLTGHHTLYMACMIAVIFAVAGFTSFSLILFGSLALGIIM
                     SVSPAFVQKYMIQLTGNDKVALGHFGSLGYWLSGFIGGIVGDKSKSTEDIKFPKSLSF
                     LRDSTVSITISMAIIYLIVAVFAGEAYIAKEISNGVNGLVYALQLAGQFAAGVFVILA
                     GVRLILGEIVPAFKGISEKLVPNSKPALDCPIVYPYAPNAVLIGFISSFVGGLVSMIV
                     MIVTGTTVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFVQGILISFLPIFLMPVL
                     GGLGFKGSTFSDADFGLTGIILGALNHVGGAIAIVIGIVVILIGLFGISFVGKSTHKE
                     G"
     gene            3773..4630
                     /locus_tag="VBD27_000788"
     CDS             3773..4630
                     /locus_tag="VBD27_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO7024817.1"
                     /translation="MTSPEERYSSLRRFATEIRLNTLETLNHLGFGHYGGSLSIVETL
                     AILYGDIMDINPEKFKESDRNYMVLSKGHAGPALYSTLYLKGFFDKTFLHSLNTNGTK
                     LPSHPDRNLTPGIDVTTGSLGQGISIATGIAYAQKIENSSYYTYTIVGDGELNEGQCW
                     EAIQFAAHHQLHHLIVFVDDNKKQLDGLTADICNPGDFVAKFEAFGFDAVRVKGDDIE
                     AIDKAIKTFQDSNSVKPKCIVLDSIKGQGVKELEELASNHHLRPDLQQKTMLERAVIS
                     LRESLEVVE"
     gene            4627..5556
                     /locus_tag="VBD27_000789"
     CDS             4627..5556
                     /locus_tag="VBD27_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEO7024818.1"
                     /translation="MRCSTKEMRLVYRDFLLQANQENKQITVLEADLSSSMSTNVLAS
                     EFGKRYINLGIMEAEMVGLAAGLAIKGYKPYLHTFGPFASRRVFDQVFLSLGYSQLSA
                     TIIGSDAGVSAEMNGGTHMPFEELGLLRLIPKATIFEVSDDIQFEAILKQTLSIDGLK
                     YIRTIRKAPTAVYEGCEDFSKGFIQLRQGKDIALVASGIMVSRAIEAADYLKELGIEA
                     SVIDLFKIKPLPEELKPLLIDQSIVTIENHNRIGGIGSALCEWLSMEKDTTVSRMGID
                     ERFGQVGQMEYLLEEYGLAVKDIVEHCKSIYKS"
     gene            5665..6924
                     /locus_tag="VBD27_000790"
     CDS             5665..6924
                     /locus_tag="VBD27_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEO7024819.1"
                     /translation="MRAWKGIVLILSSIVVTLVAWQNAGLSEFVVPGLALTSLSLTFL
                     LSTKFRILESYFQGIENMYFYHKVMAVFSMILLLLHKIGLVQGGHGSEFAKTIGSAGL
                     YLFLSIVFVAYFGNFLKYEIWRFIHRFVYLVYILGLVHTFLILGDRILGNTLLSLIVL
                     GYAVIGVISGFYIIFLYSRMRFRRVGYVQKVTHLNHDTTEIEIAMKRPYRYDYGQFTF
                     LKIYQAGFESAAHPFSISGGHDRVIFLTVKASGDYTKSIYKQLKVGTKIALDRAYGHM
                     LFDKDKKEQVWIAGGIGITPFISFIRENSILTKRVDFFYTFSNQDNLIYQDMLESYAK
                     ANPNFKLHLNNSSLQGRLDFSQSVFEGQPTIFMCGPTSMSSTYAKVFRQKDAKSRLVY
                     EGFSFRDSWLSIFLLKTFDKVYSNLIK"
     gene            7012..7281
                     /gene="rpsO"
                     /locus_tag="VBD27_000791"
     CDS             7012..7281
                     /gene="rpsO"
                     /locus_tag="VBD27_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO7024820.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            7661..9790
                     /gene="pnp"
                     /locus_tag="VBD27_000792"
     CDS             7661..9790
                     /gene="pnp"
                     /locus_tag="VBD27_000792"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO7024821.1"
                     /translation="MSKQVFEMIFAGKKLVVETGQVAKQANGSVVVRYGDSTVLTAAV
                     MSKKMSTGDFFPLQVNYEEKMYAAGKFPGGFNKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSFDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVAYVDGNFI
                     INPTAQEQEASALELTVAGTKEAINMVESGAKELSEEIMLEALLKGHEAVCELIAFQE
                     EIVTAIGKEKAEVELLQVDPELQAEIIATHNIALQAAVQVEEKKAREAATEAVKEVVI
                     GEYEARYAEHEEYDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRRVDEIRPLDAVI
                     DFLPQVHGSGLFTRGQTQALSVLTLAPMGEAQIIDGLTPEYKKRFMHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIEGITPQILEEALAQAKKARFEILDVLHGAIAESRPQLAPTAPKIDMIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDEEGNVSIFSSDQAAIDRTKDIIASLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTANVADVLEIGEEVDVKVIKI
                     DDKGRVDASMKALLPRPPKADNPKKES"
     gene            9792..10544
                     /locus_tag="VBD27_000793"
     CDS             9792..10544
                     /locus_tag="VBD27_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEO7024822.1"
                     /translation="MTSTNELDIRLRAFINAPDNFLDSIGLVNALHHSTVWASKEPYA
                     IQVDGQEVVPVFTDITDLNHFKEEQESARDMFWESRRSLDVLDEAISHGLAGLVYNLK
                     KEGDFGNSTIFYCEDMVQFMNNYTTILNQLLNEDNIVADIMDKTYLVPAFVHPREEGS
                     FDRFFPTMSTPEGKSYVPVFSNLLSFEKWYNHNDFGGAFRKAQGVILAWTIDDIYKPR
                     NGENEIDDTFGVAINPFDEQQVLVDWSDVEND"
     gene            10553..11137
                     /gene="cysE"
                     /locus_tag="VBD27_000794"
     CDS             10553..11137
                     /gene="cysE"
                     /locus_tag="VBD27_000794"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEO7024823.1"
                     /translation="MGWWKESIAIVKEQDPAARSSLEVILTYPGIKALAAHRLSHFLW
                     NHNFKLLARMHSQFWRFWTQIEIHPGATISEGVFIDHGSGLVIGETAIVEKGAMLYHG
                     VTLGGTGKDKGKRHPTIRKGALISAHSQIIGPIEVGENAKVGAAAVVLADVPADVTVV
                     GVPAKVVRVHGQKDDLQIRSIEHDREESYYSSKL"
     gene            11147..11329
                     /locus_tag="VBD27_000795"
     CDS             11147..11329
                     /locus_tag="VBD27_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024824.1"
                     /translation="MKDYVTTQILSILGILAGACLSYLKFAQGNSLLGIIWAAFALMY
                     IVRLYKAYQKQKKEKP"
     gene            11326..12669
                     /locus_tag="VBD27_000796"
     CDS             11326..12669
                     /locus_tag="VBD27_000796"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO7024825.1"
                     /translation="MIKIYDTMTRSLQDFIPLNEGKVNMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYCGYQVNYISNFTDVDDKIIKGAAEAGMDTKSFSDKFISAFMEDVAA
                     LGVKPATKNPRVIDYMDEIIDFVKVLVDKEFAYEANGDVYFRVSKSHHYAKLANKTLE
                     DLEIGASGRVDGEGEIKENPLDFALWKSAKSGEVSWESPWGKGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVNVDNEKMSKSLGNF
                     ITVHDMLKSVDGQVIRFFLATQQYRKPVNFTEKAVHDAEVNLKYLKNTFNLPIQENAN
                     DEELEQFVKAFQEAMDDDFNTANGITVIFEMAKWINSGHYTSKVKETLAELLEIFGIV
                     FQEEVLDADIESLIEQRQEARANRDFATADRIRDELAKQGIKLLDTKDGVRWTRD"
     gene            12662..13048
                     /locus_tag="VBD27_000797"
     CDS             12662..13048
                     /locus_tag="VBD27_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEO7024826.1"
                     /translation="MIDVRLINGIALAFEGDAVYSLYIRRHLIMQGFTKPNQLHRKAT
                     QYVSANAQALLINAMLEENILTDEEQLIYKRGRNANSHTKAKNADIITYRMSTGFEAL
                     MGYLDMTGQIKRLETLIQWCIETIEK"
     gene            13151..13906
                     /gene="rlmB"
                     /locus_tag="VBD27_000798"
     CDS             13151..13906
                     /gene="rlmB"
                     /locus_tag="VBD27_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO7024827.1"
                     /translation="MTMKDKQFKEESSDLVYGLHAVTESLRANTGNKLYLQDDLRGKN
                     VDKVKALATEKKVSISWTPKKILSDMTNGGVHQGFVLKVSEFAYADLSEIMTKAENEE
                     NPLILILDGLTDPHNLGSILRTADATNVTGIIIPKHRSVGVTPVVSKTSTGAVEHVPI
                     ARVTNLSQTLDTLKDKDFWIFGTDMNGTPSHKWNTKGKLALVIGNEGKGISHNIKKQV
                     DEMITIPMNGHVQSLNASVAAAILMYEVFRNRL"
     gene            13903..14421
                     /locus_tag="VBD27_000799"
     CDS             13903..14421
                     /locus_tag="VBD27_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEO7024828.1"
                     /translation="MKKHSILLVDGYNMIAFWKDTRQLFKSNRLEEAREVLLRKLNHY
                     AHFEHIDIICVFDAQYVPGVRQRYDQYKISVIFTEEDETADSYIERAAAELNQSVLNL
                     VSVATSDLNEQWTIFSQGALRVSARELEQRVATVKSDLDKMSSQIDLSTPKLRPWNDE
                     QLGKLKDFLDGM"
     gene            14514..15374
                     /locus_tag="VBD27_000800"
     CDS             14514..15374
                     /locus_tag="VBD27_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7024829.1"
                     /translation="MTFKILTDSTSDLDEKWAQEHNVDIIGLTIELDGKTYETVGDEK
                     ITSDFLLERMQEGAKPTTSQINVGQFEEVFSTYAENDHALLYLALSSHLSGTYQSATI
                     AREMVLDKYPDAQIEIVDTMAASCGEGVLAMLATKERQEGKSLEEVKQKIESLLPKLN
                     TYFLVDDLNHLMRSGRLSKGAAIIGSVAKIKPLLKLDSEGKLVPFAKTRGRKKGIKEI
                     VTQATKTLSYSTLIIAYSGEKDSAQVMKEQLLADERIEEVIIRPLGPVISAHVGSGAL
                     ALFSLGEENR"
     gene            15911..16033
                     /locus_tag="VBD27_000801"
     CDS             15911..16033
                     /locus_tag="VBD27_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7024830.1"
                     /translation="MKNLRLKTARASMDLLQQSLAEKVGVSCQTIAAIEKGGYN"
     gene            16255..16701
                     /gene="rplM"
                     /locus_tag="VBD27_000802"
     CDS             16255..16701
                     /gene="rplM"
                     /locus_tag="VBD27_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO7024831.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKASDKIYYTHSMYPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            16722..17114
                     /gene="rpsI"
                     /locus_tag="VBD27_000803"
     CDS             16722..17114
                     /gene="rpsI"
                     /locus_tag="VBD27_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO7024832.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITINKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVVGGGYAGQSGAIRHGISRALLEVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
BASE COUNT         5540 a         2699 c         3585 g         5441 t
ORIGIN      
        1 tagtagtgcc agattgggct caaatgacct agaaaaatgt aaacggttac tataaaataa
       61 aaatagaaag gaaaacaaat ggttgataat aagactgttg tgataatgtt ggtattttta
      121 gctcggaaaa atctttcctt gtatgagtta acagtgcaaa caaagttttc aataaaagtt
      181 atcatagagc aaattaacta tctcaatagt tttttagcaa aaaatcacct accagctatt
      241 gctcactctg ctggacgtta tcagttatta ggagatgaaa aagagcatga taaaattgtc
      301 tccttattag aagctgagca attttaccta actcaggaag aaagggtatg tctgatttac
      361 ctttatagct tttgccgaag agagtttgtc tccaatgtgc attatcaaga ttttttgaaa
      421 gtaagtaaga atactacttt atcagatatc aagatgctac gttctaaatt agcaaaaaga
      481 ggtatctcac tcacttatac aagagcaaag ggatatagtc tagtaggaga tgagatggat
      541 aagcatcaag ttgcttttca gatgataact caactactag aatctcccat tggtttttgg
      601 tctttgaatt atatcttgtc ttcttggaaa ttcgctctct catatgagaa gttagaaaag
      661 acggtagaat atttctatga atcttttcaa ctttctccaa tacaagatag gcttgagaaa
      721 tctttatatt ttatcatttt aatactgtgt cgttatcaaa ggtcagtaga tcgtgttttg
      781 cagggttctc ctattgtttc tgaacaatta aaagagttaa cgactattat tgtgactaat
      841 ttaagtcagg atatttcctt atctaaacca ttagatcaga aagaaaaaga ttatattacc
      901 ttgatcttat ctggctgttt tgaaggtgag ggaactaaag atgatgattt ctttgaagca
      961 ttggcaaaag caatcgttga tgagatggaa actgtttcac ttcttaattt ttccaataaa
     1021 gaagagttat tacaaggtct gaagcgtcat attattccag cttatttccg attgaaatat
     1081 ggtttaactg gcgatagtgg ttatacacaa aatattaaag agcattacag tgatttattt
     1141 ctattggtta aaaaggcact acgtcctttg gaagagcaag taggattaat acccgatagt
     1201 gaaatttctt acttcgtgat tcactttgga ggctatctta gacaatcagg tgatacacaa
     1261 tcaatgtctt ataaagcttt gatactctgt ccaaacggcg ttagttcatc cttagtgatt
     1321 aaggaaaaat tgcgtggatt atttcctcaa attcattttc atcgtgtctc taaaattgag
     1381 cagctgaaac tgattgacaa tcaaacttac gatatggtct tttctactat atttgtagaa
     1441 acaaaaaaac ctaattatct agtttcgctg atgatgacag cagagcaagt acaacagctc
     1501 aaagaactgg ttatttcaga ttttcccaaa gcttgccttg acgatttcca acttgatcaa
     1561 ttaatagcaa ctataaaaaa atacgctcat gttcattgtg aagaggaatt aaaattggct
     1621 ttgaggacaa tggtaaaaca agatatattt agaaaggatg tgaggccctt gttgcaccaa
     1681 cttataacag aggaaactta tcagacaagt tctgaacaaa tgaattggaa agaggcaatt
     1741 cgtttagcgg caaaaccctt gttagcttct ggaaaaatta cggaaagtta cccagaagct
     1801 atgattgaaa aggtcgaaga gtttggtcct tttatcaatc ttggtaaagg tattgctatt
     1861 ccacatgcca gaccagaaga tggggtaaac tctgtcggta tgtcaatgct tgttttggaa
     1921 cagcctattt atttattgga tgaccccaaa caggaaattt atctcttatt atgcatagca
     1981 gctattgata atgaaacgca tttgaaagct ctatcgcatt tgacaacaat acttcgtgat
     2041 aataataatg ttaaagcttt gttagcttca cgtgggtatc aagatattga aatgattata
     2101 aaacaggagg attaaagatg ttaagaattg gaacagcatg tggatcaggt ctaggttcaa
     2161 gttttatggt acaaatgaat attgagtcta ttttgaagga tttgggggtt tctgatgttg
     2221 aagttgaaca ttatgattta ggtggagcag atccaagcgc agcagatgtt tggattgttg
     2281 gtcgtgattt ggaggattca gctggtcacc tgggggatgt tcgtatttta aacagtatca
     2341 ttgatatgga tgaattgaga gaattagtga caggtatttg ccaagaaaaa ggcttaatct
     2401 aggaggttga tgccatgaaa ggtctattgg attttttagt taatattgcc agaacgccag
     2461 ctattttagt cgccttgata gccattatcg gtttagtact gcagaaaaaa ggtgttcctg
     2521 atattgtaaa aggtggaata aaaacatttg ttggcttctt agtggtttct ggaggtgcag
     2581 ggatagtcca aaattccttg aatccatttg gaaaaatgtt tgaacatgct tttcatttgg
     2641 tgggggtagt tcctaataat gaagccattg tagcagtagc tcttacgaag tatggctcag
     2701 caactgcttt gattatgtta gcgggaatga tttttaatat tttaattgct cgttttacaa
     2761 aatttaaata tatttttctg acaggacatc atacattgta tatggcttgt atgattgctg
     2821 ttatttttgc cgtagcaggc tttacttcgt tctcgctcat cctttttgga agtctagcgt
     2881 tagggattat tatgagcgta tctcctgcat ttgtgcaaaa atacatgatt caactgacag
     2941 gtaatgataa ggtggcactt gggcactttg gttctttagg ctattggttg agtggattta
     3001 ttggtggaat tgttggagat aaatcaaaat caacagaaga tattaaattt ccaaagagtt
     3061 tatcattttt acgtgacagt accgtaagta ttactatctc aatggcaatt atttacctta
     3121 tcgttgcagt gtttgctggt gaggcttata ttgcaaaaga aataagtaac ggtgtcaatg
     3181 gtcttgttta tgccctccaa ctagctgggc aatttgcagc aggtgtattc gttattttag
     3241 caggtgtacg ccttatccta ggtgaaattg taccagcctt taagggaatc tcagaaaaat
     3301 tggttccaaa ttcaaaacca gccttggatt gtccaattgt ttacccttat gcacctaatg
     3361 cagtattgat tggttttatc tcaagttttg ttggtggatt ggttagtatg atagttatga
     3421 ttgtgactgg cactactgtc attttaccag gggtcgttcc tcatttcttc tgtggggcaa
     3481 cggcaggtgt tatcggtaat gcttctggtg gtgtgagagg agcgactatt ggtgcatttg
     3541 ttcaaggtat cctcatcagt ttcttgccga ttttcttgat gcctgtacta ggtggacttg
     3601 gtttcaaagg ttcaacattc tcagatgctg attttggttt gaccggtatc attcttggcg
     3661 ctcttaatca tgtcggcggt gcgatagcta ttgttattgg tattgtagtt atcttaattg
     3721 gactttttgg catttcattt gttggaaaat caacccataa ggaaggttaa taatgacatc
     3781 accagaagaa agatattcat ctctaagacg atttgcaacg gagattcgtt taaatacact
     3841 agaaacgcta aaccatttag gattcggtca ctatgggggt agtttatcaa ttgttgagac
     3901 tttggctatt ctgtacggtg atattatgga tataaaccct gaaaaattca aagaaagtga
     3961 tcggaattat atggtcttat caaaaggaca tgctggtcca gccttgtata gtacactcta
     4021 tttaaaaggt ttttttgata aaacattcct tcattcgctc aatacaaacg gtaccaaact
     4081 accttcgcat cctgaccgca atttaactcc tggtatagat gtaacgacag gctcattagg
     4141 tcaaggcatt agtattgcaa cgggaattgc ttatgctcaa aagattgaga attcaagcta
     4201 ttatacttat actattgtag gtgatggtga attaaatgaa ggacaatgtt gggaggctat
     4261 acaatttgct gcgcatcatc aactgcacca tttaattgtt tttgtagatg ataataaaaa
     4321 acaattagat ggtttgacag ctgatatttg taatcctgga gactttgttg ctaaatttga
     4381 agcttttgga tttgatgcag tacgtgtaaa aggagatgac attgaggcaa ttgacaaagc
     4441 tattaaaact tttcaagatt caaatagtgt caaaccaaaa tgcattgttt tagatagtat
     4501 caagggacaa ggtgtgaaag agttggagga gttagcttct aatcatcatt tacgaccaga
     4561 tttacaacaa aaaacaatgt tagagcgagc ggtgataagc ttgagagaaa gtttggaggt
     4621 ggtagaatga gatgttcaac caaagaaatg aggcttgtct atcgtgattt cctccttcaa
     4681 gctaatcaag aaaataaaca aattaccgtt ttagaagccg atttgtcgag ttcaatgtca
     4741 acaaatgtac tggcatcaga atttggaaaa cgttatatta atctaggtat tatggaggct
     4801 gagatggtag gtcttgcagc agggctcgcc attaaaggtt acaagccata ccttcatact
     4861 tttggtcctt tcgcgtcaag gcgtgtcttt gatcaagtat ttttatcgtt aggttactca
     4921 caattatcag ctacgattat tggctcagat gcaggagtta gcgcagaaat gaatggcgga
     4981 actcatatgc cttttgaaga gttaggtcta ctaaggttaa ttccaaaggc gactattttt
     5041 gaggttagtg atgatattca gtttgaagca attttaaaac aaacattaag tattgatggt
     5101 ctaaaataca ttagaactat tcgtaaggcc cccactgcag tttatgaggg atgcgaagat
     5161 ttttcaaaag gttttataca attacgccag ggaaaagata ttgctcttgt agcaagtggt
     5221 attatggttt ctagagcgat agaagcagcc gactacctca aagaactagg gattgaggcc
     5281 tcagttatcg acctttttaa aattaagccc cttccagaag aactgaaacc attactgata
     5341 gatcaatcca ttgtaactat tgagaatcat aaccgtattg gcggtatcgg aagtgcatta
     5401 tgcgaatggc ttagtatgga gaaagacact actgttagtc gtatgggtat tgatgagcga
     5461 tttggtcagg taggtcagat ggaatattta ttagaggagt atggactcgc tgttaaagat
     5521 atagtagagc attgcaaatc aatttataaa tcataacaaa aaggcaacat tggagttgcc
     5581 tttttgttat tttctgaaat ttttaagttt tgtataagta tcttgtggta ttttatcctt
     5641 actaaaaata aaggggtggt cgttatgcga gcttggaaag gtattgtttt aattttatct
     5701 agcatagtgg tgacactagt agcatggcaa aatgcaggat taagtgaatt tgttgtacca
     5761 ggattagctt tgacaagttt atcgctaaca ttcctcttat caaccaaatt taggatactt
     5821 gaaagttact ttcaaggtat tgaaaatatg tatttttacc acaaggtaat ggctgtattt
     5881 tcaatgattt tgcttctatt gcacaaaatt ggtcttgtac aaggcggaca tggaagtgag
     5941 tttgcaaaga ctattggcag tgcgggatta tatctttttc taagtattgt ttttgtagct
     6001 tatttcggaa attttttaaa gtatgaaatt tggcgtttta ttcatcgctt tgtttattta
     6061 gtctatattt tgggattggt acatactttt ctgattttag gagataggat actagggaat
     6121 actttactca gtttaatcgt attaggttat gctgtcattg gggttatatc tggattttat
     6181 attattttcc tatattctag aatgcgtttt cgtcgtgtag ggtacgttca aaaggttact
     6241 catcttaacc atgatacaac tgagattgaa atagcaatga agcgtcctta tcgatatgac
     6301 tatggacaat ttactttttt aaaaatttat caagccggct ttgaatcagc agctcatcct
     6361 ttctcgatat cgggtggtca tgatagagtg atatttttaa ctgttaaagc ttcaggagat
     6421 tatacgaaat ccatttacaa acagctaaaa gttgggacaa aaatagcctt agatagagct
     6481 tatggtcata tgttatttga taaagacaag aaagaacagg tttggattgc aggaggtatc
     6541 ggcatcactc ccttcatttc atttattaga gaaaatagta ttttgactaa gagagttgac
     6601 ttcttttata cattttcaaa tcaagacaat ttgatttatc aagatatgtt agaatcctat
     6661 gctaaagcca atcctaattt taaacttcac ttaaataata gtagcctcca aggaagactt
     6721 gattttagtc agtctgtttt tgaaggacaa ccaacgatat ttatgtgtgg gccaactagt
     6781 atgtcatcta cttatgctaa ggtctttcgg caaaaagatg caaagtctcg tttagtctat
     6841 gaaggatttt cctttagaga cagttggtta tccatatttc tattaaaaac ttttgacaaa
     6901 gtttattcta atttgataaa ataagagggt ctataagaca aaccaacttt ttcttggttt
     6961 taggagtgcc aatcctaaga ctcggatgaa gcaataaaag gagaatatat tatggcaatc
     7021 tcaaaagaga aaaaaaatga aatcattgct caatatgcac gtcacgaagg tgatacagga
     7081 tcagttgaag ttcaagtagc agttcttact tgggaaatca accaccttaa tgaccacatc
     7141 aaacaacaca aaaaagacca cgctacttac cgtggattga tgaaaaaaat tggtcaccgt
     7201 cgtaacttat tagcatacct acgtcgtaca gatgttaacc gttaccgtga attgattcaa
     7261 tcacttggac ttcgtcgtta atagacaaca gagtcaagta aaaacgctag aaaattttct
     7321 agtgtttttt gttttaaacg tactatgaac gtaagtactt tgagggggcc aacagtaaga
     7381 aagtgtttta acctatgaag agggttatta agcaagctaa gattaaccct agaaatttat
     7441 tttaatcatt gtaacaaggt ctgattgcta gtattaataa tcagactttt tacgtttaca
     7501 ttaatgaatg ataatacagc ttcccccacg gattttcgct actcaatttt atagaattat
     7561 gttagaataa agcttatacg tggttttatg caaagttgag tttagaacag ctagattgaa
     7621 ctttgtagga aacgcgtaaa caaaaaatat ggagaataat atgtcaaaac aagtctttga
     7681 aatgattttt gctggtaaaa aattagttgt tgaaactggc caagtggcta aacaagcaaa
     7741 tggatcagtt gtcgttcgtt atggagattc tactgtactt acagcagcag ttatgtctaa
     7801 gaaaatgtca acaggtgatt ttttcccact tcaagtcaat tacgaagaaa aaatgtatgc
     7861 tgctgggaaa tttccgggtg gttttaacaa acgtgaaggg cgtccaagta cggatgctac
     7921 cttaacagca cgtcttattg atagaccaat ccgtccgatg tttgctgagg gatttcgtaa
     7981 cgaagtccag gtcatcaata ctgttctttc atttgatgaa aatgctagcg caccaatggc
     8041 tgctatgttt ggttcatcat tagcattatc gatttccgat attcctttca atgggccaat
     8101 tgctggagtt caagttgctt atgttgatgg caattttatt attaatccga cagcacaaga
     8161 acaagaggct tctgcgttag aattaacggt tgcaggtact aaagaagcga ttaacatggt
     8221 tgaatctgga gcgaaagaac tttcagaaga aatcatgcta gaggctcttc ttaaaggaca
     8281 tgaggctgtc tgtgaattaa tcgcctttca agaagagatt gtaactgcta ttggtaaaga
     8341 aaaagccgaa gtagaacttc ttcaagttga tccagaactt caagcagaaa ttattgctac
     8401 tcataacata gctttacagg ctgcagtaca agttgaagag aaaaaggcta gagaagcagc
     8461 tacagaagca gttaaagaag tagttatagg tgaatatgag gcgcgttacg ctgaacatga
     8521 agaatatgat cgcattatgc gtgatgttgc tgaaatttta gaacaaatgg aacatgcaga
     8581 agttcgtcgc ctcattacag aagataaggt tcgtcctgat ggacgtcgtg tggatgagat
     8641 tcgtccatta gatgcagtga ttgatttttt accacaagtt catggatcag gtctctttac
     8701 acgaggacaa acccaagcat tatcagtatt aacgttagca cctatgggtg aagcacaaat
     8761 cattgatggg ctaacaccag agtataagaa acgctttatg caccactata acttcccaca
     8821 atattcagtt ggtgagacgg gacgttatgg agctgcaggg cgtcgtgaaa ttggtcacgg
     8881 tgctttagga gagcgtgcat tagaacaagt ccttccaagc ttggaggagt ttccgtatgc
     8941 tatccgacta gttgcagaag ttttagaatc aaacggttca tcatctcagg cttctatatg
     9001 tgctggaacg cttgctctta tggcaggtgg tgtgcctata aaagctccgg ttgctggtat
     9061 tgccatggga ctcatatcag atggaacaaa ctacacagtc cttactgata ttcaaggatt
     9121 agaagaccat tttggcgata tggactttaa ggttgcgggt acacgtgaag ggattacagc
     9181 acttcaaatg gatattaaaa ttgaaggtat tacccctcaa attttggaag aagctcttgc
     9241 gcaagctaaa aaagcacgct ttgaaattct tgatgtcctt catggagcta tcgcagagtc
     9301 acgtccacaa ttagcaccaa cggcacctaa gattgatatg ataaaaatcg atgttgataa
     9361 gattaaagtt gtcatcggta aaggcggcga aactatcgat aaaatcatcg ctgaaacagg
     9421 tgttaagatt gatattgatg aagaaggaaa tgtatctatc ttctctagtg atcaggctgc
     9481 tattgatcgt acgaaagaca ttattgcaag tttagtacgt gaagcaaaag tgggagaagt
     9541 ttaccatgct aaagtagttc gtatcgagaa attcggtgcc tttgtcaacc tctttgataa
     9601 aacagatgca ttggtacata tttcagaaat tgcatggaca cgtacagcaa atgtagctga
     9661 tgttcttgaa attggtgaag aagttgatgt gaaagtcatc aaaattgatg ataaagggcg
     9721 agttgatgcc tctatgaaag cattattacc acgtcctcca aaagctgaca atcctaaaaa
     9781 ggaatcttaa tatgacaagt acaaacgagt tagacattag attgcgtgct tttatcaatg
     9841 caccggataa ttttttagat agtataggtc ttgtgaatgc cttgcaccac tctacggttt
     9901 gggcaagcaa ggaaccctat gctattcaag tagatggtca agaagttgtt cctgttttta
     9961 cggatattac tgaccttaat catttcaaag aggagcaaga aagtgctcga gatatgtttt
    10021 gggagagtcg tcgtagttta gacgttcttg atgaagctat ttcacatggc ttagcaggat
    10081 tggtctataa tttaaaaaaa gaaggcgatt ttgggaactc gactattttc tactgtgaag
    10141 atatggtgca atttatgaac aattacacca caatcttgaa tcagttgtta aatgaagata
    10201 acattgttgc cgatatcatg gataaaacct acttggtacc tgcctttgtc catccacgtg
    10261 aagaaggtag ttttgaccgt tttttcccta caatgtctac ccccgaagga aaaagttatg
    10321 ttccagtatt ctcaaacctt ttaagttttg aaaaatggta taaccataat gattttggtg
    10381 gtgcttttag gaaagcacaa ggagtaattt tagcctggac gattgacgat atttataaac
    10441 cgcgtaatgg agaaaatgag attgatgata cctttggagt agccatcaat ccatttgatg
    10501 aacaacaagt tttagttgat tggtctgatg tagaaaacga ttgaggaaaa ctatggggtg
    10561 gtggaaagaa agtatagcta ttgtcaaaga acaagatcca gctgctcgta gttctctaga
    10621 agttatttta acttatccag gaataaaagc tcttgcagct cataggctat ctcatttttt
    10681 atggaatcat aattttaaat tgttagcacg tatgcacagt caattttggc gtttctggac
    10741 acaaattgaa attcatcccg gagcgacgat ttcggaaggg gtttttattg accacggttc
    10801 aggattggtc attggagaaa ctgccattgt agagaaaggt gctatgctat accatggggt
    10861 tacattgggt ggcacaggta aagacaaagg caaacgacac ccaaccatac gtaaaggtgc
    10921 gctaatttca gcacattctc aaatcatcgg tccaattgag gttggagaaa atgctaaagt
    10981 cggtgctgca gcagttgtcc ttgcagatgt gccagcagac gtaacagtag ttggtgttcc
    11041 agctaaagtt gtccgtgtcc atggtcaaaa ggatgatctt caaatccgaa gtattgaaca
    11101 tgatcgagaa gaaagttatt attcgtccaa actttaggag gtatttgtga aggattatgt
    11161 aacaactcaa atattatcaa ttttaggtat actagcagga gcttgtttaa gctatttaaa
    11221 gtttgcacaa ggtaactctc tcttaggaat tatttgggct gcctttgctt taatgtatat
    11281 tgttcgtttg tataaagctt atcaaaaaca gaaaaaggaa aaaccatgat aaaaatttac
    11341 gacactatga ctcgcagttt acaagatttt atacctctca atgaaggtaa agtcaacatg
    11401 tacgtctgtg ggccaacagt ttataactat atacacatcg ggaatgcccg cagtgtagta
    11461 gcttttgata ctattcgtcg ttattttgag tattgtggtt accaagtcaa ttatatttct
    11521 aactttactg atgttgatga taaaattata aaaggtgcag ctgaagcagg tatggataca
    11581 aaatcctttt cagataagtt tatttcagca tttatggaag atgttgctgc attaggtgtc
    11641 aaacctgcta caaaaaatcc tcgagttatt gactatatgg atgagattat tgattttgtt
    11701 aaagttttag ttgataagga atttgcctat gaagctaatg gagatgttta ttttagagta
    11761 agtaaatcac atcattatgc aaaattagcc aataaaactt tggaagattt agagattggt
    11821 gcaagtggtc gtgttgatgg ggaaggagag attaaagaga atcctttaga ttttgcactt
    11881 tggaaatcag ctaaatctgg ggaagtctca tgggaaagtc cttgggggaa agggcgccca
    11941 gggtggcata ttgaatgttc tgttatggca actgagatcc ttggtgatac tattgatatc
    12001 catggtggag gagctgattt agaatttcca catcacacga atgagattgc acaatctgaa
    12061 gctaaaacag gtaagacctt tgcaaattac tggatgcata atggttttgt taacgttgat
    12121 aacgagaaaa tgtctaaatc tcttggtaac tttattactg ttcacgatat gttgaagagc
    12181 gttgacggac aagtaatacg ttttttctta gcgacacaac agtatcgtaa acccgtgaat
    12241 ttcactgaaa aggccgttca tgatgcggaa gttaacctta aatatttaaa aaatactttc
    12301 aacttaccaa tccaagagaa tgctaatgat gaagaattag aacaatttgt gaaagcattt
    12361 caagaagcga tggatgatga ttttaataca gcaaacggta ttactgttat ctttgaaatg
    12421 gcaaaatgga ttaattcagg acactatact tcaaaagtta aggaaaccct tgctgaatta
    12481 ttagaaattt ttggtattgt attccaagag gaagttcttg atgctgatat tgaatcttta
    12541 atagaacaac gtcaagaagc acgagctaat cgtgattttg caactgctga tcgtattaga
    12601 gatgaattag caaaacaagg aattaaattg ttagatacta aggatggcgt gaggtggact
    12661 cgtgattgat gttcgtttga ttaatggtat tgctttagct tttgaaggag atgcagttta
    12721 ttctttgtat atccgacgcc atttaattat gcagggtttt accaaaccta atcagttaca
    12781 tcggaaagca acccaatatg tttctgctaa tgcccaagca ttgttaatta atgccatgtt
    12841 agaagaaaat attttaactg atgaagagca gttaatttat aaacgaggac gtaatgcgaa
    12901 tagtcataca aaagctaaaa atgctgatat tattacctat cgtatgtcta caggttttga
    12961 ggctcttatg gggtacttgg atatgactgg tcagataaag cgcttggaaa ctctaataca
    13021 gtggtgtatt gaaaccattg aaaaataaac aaaaagagag gctatatcct ctctttttta
    13081 tagagacatt aggatttgtt ggtaaaatat tgctataaaa agctttatgc tatagcattc
    13141 atgatataat atgactatga aagataaaca atttaaagaa gaatcaagtg accttgtcta
    13201 tggcttacat gctgtgacag agagtttaag agctaatact ggtaataaat tatacttgca
    13261 agatgactta agaggaaaga acgttgataa agtcaaagca ctagcgactg aaaaaaaagt
    13321 atccatttcc tggactccta aaaaaatctt atcagacatg acaaatggtg gagttcacca
    13381 aggattcgtt ctaaaagttt cggaatttgc ctatgcggat ttatcagaga taatgacaaa
    13441 ggcagagaat gaagaaaatc ctctaatttt aatccttgat ggtctgaccg accctcataa
    13501 cctaggttct attctacgca cagctgatgc aaccaatgta acaggcatta tcatccccaa
    13561 acatcgttct gttggagtga ccccggtggt ttcaaagaca tctacaggag ctgttgaaca
    13621 tgttcctatc gcaagggtaa ctaatctcag tcaaactttg gatactttaa aagataagga
    13681 cttctggata tttggcactg atatgaatgg gacaccatcg cataagtgga ataccaaagg
    13741 taaattagcc ctagttattg gtaatgaagg aaaaggtatt tctcataata tcaaaaagca
    13801 agtagatgaa atgattacaa ttcccatgaa tggtcatgtt cagagtttaa atgccagtgt
    13861 cgcagctgct attttgatgt atgaagtatt tagaaaccgc ctatgaaaaa acattctatt
    13921 ttactcgttg atggctacaa tatgattgct ttttggaaag atactcgaca gctttttaaa
    13981 agcaatcgtt tagaagaggc aagggaagta ttacttagaa agctaaatca ttacgcacat
    14041 ttcgaacaca ttgatattat ttgtgtattt gatgcccagt atgttcctgg tgtacgtcaa
    14101 cgttatgacc aatacaaaat atccgttatc tttactgagg aggacgaaac tgcggatagc
    14161 tatattgaac gagcagcggc agaacttaat caatctgtgt taaatttagt ctccgttgct
    14221 accagtgatt taaatgaaca atggactata ttttctcagg gtgctttacg tgtctcagct
    14281 agagaattgg aacagcgtgt agcgacagta aaatcagatt tggataagat gtccagtcaa
    14341 atcgatttga gtacgccaaa gttaaggccg tggaatgatg aacaactagg aaaactcaag
    14401 gattttttag atggtatgta aaaactaaca ctttattagc cagccaacaa tttttttgat
    14461 ataatattgg aactactttg attatcaaat aaaaataata gattgaggta acaatgactt
    14521 ttaaaatact aacggattcg acatcggatt tagatgaaaa gtgggctcaa gagcacaatg
    14581 tcgatattat aggtttaacc attgaacttg atggaaagac ttatgagaca gtcggagacg
    14641 aaaaaattac tagtgacttc ttgttggagc gtatgcaaga gggagcaaag cctacaacaa
    14701 gccaaattaa cgttggtcag tttgaagaag ttttttcaac ctatgcagaa aatgaccatg
    14761 ccttgttata tttagcgcta tcttcacatt tatcagggac ttaccagagt gcaactattg
    14821 cgcgtgagat ggtcttagac aaatatcctg atgctcaaat tgagattgtg gatacaatgg
    14881 cagcttcatg tggtgagggt gtcttagcga tgttagcaac taaagagcga caagaaggta
    14941 agtctttaga agaagtgaaa caaaaaattg aatcacttct ccctaaatta aacacatact
    15001 ttttagttga cgatctcaac catttaatgc gtagtggtcg cttatcaaaa ggtgcagcta
    15061 tcattggaag tgttgctaag attaaaccat tacttaagtt agatagtgag ggtaaattag
    15121 taccatttgc caagacaagg ggccgtaaaa aaggtattaa agagattgtg actcaggcaa
    15181 caaaaacact atcttatagc actttaatta ttgcttatag tggagagaaa gattcagctc
    15241 aagttatgaa agaacagtta ttagcagatg agcgtattga agaagttatt attcgtccat
    15301 tagggccagt tatttcagct catgtgggct caggtgcttt agctctattc tctttagggg
    15361 aagaaaaccg ataacaaatc aaagggcata acagtacgtt atgttctttt agtatacatt
    15421 ttataaaatg tattttatat gatgctatga aactataaaa tagtaggaga catattatga
    15481 aaaataaaat aagaactatc ctcaatatga tgaacgacaa caacaattgg ttttacaaag
    15541 ttatcaaaaa tgtttttaga cgatgttttt tatcttatgg cttgaaagtg tttttggaaa
    15601 gctaataaag ggaagcgtgt cacctgattt tataataata ttaactttat ttagtggtat
    15661 ttctattttg ttacgacttc taatctaaaa agagtctctc cgtttgttga tgaaagattt
    15721 tggaaaatgt ttttgttgct ggcgcatttg ccctcatagg aataactatt ttattgatgc
    15781 ttttactagg aagttgatat tgctacttat ttttcaaggg gaaatgatgc aattttagca
    15841 gttataggaa tatcattgat agctatggga acttcagtat actaccttaa gtataaggac
    15901 agatagtaat atgaaaaatt tacgcttaaa gacagctaga gctagtatgg atttattaca
    15961 acagtctcta gctgaaaagg tcggtgtatc atgtcagaca atagccgcca ttgaaaaagg
    16021 aggctataat taagcgatta aattgtgtca agctttgaat aaaactgtag atgatttatt
    16081 ttgggatgaa aattgataaa ataaaagaaa taacttgacg aatgctacct tattttgata
    16141 tgattataag cggtattgtt taccccattt gtaaggcccc ggaacctttc aaatactctc
    16201 gtggaccgga acatccacac ctgtaaacaa aaacgaattc gtataggaga aatcatgaac
    16261 aaaacaactt tcatggctaa accaggccaa gttgaacgca aatggtacgt tgttgacgca
    16321 acagatgtac ctcttggacg tctttctgca gtagttgcta gcgtacttcg cggaaaaaac
    16381 aaaccaactt tcacaccaca cactgataca ggtgactttg tgattgttat caatgctgaa
    16441 aaagttaaat taactggtaa aaaagcatca gataaaatct actacactca ctcaatgtat
    16501 ccaggtggtt tgaaacaaat ctcagcaggt gaacttcgtt ctaaaaatgc tgttcgtttg
    16561 attgaaaaat cagttaaagg catgcttcca cataacactc ttggacgtgc acaaggtatg
    16621 aaattgaaag tatttgttgg cggtgagcat acacatgctg cacaacaacc agaagtactt
    16681 gatatctcag gacttatcta agaggaaagg agcattatat aatggcacaa gcacaatatg
    16741 caggtactgg tcgccgtaaa aacgctgttg cacgcgttcg tttggttcca ggtactggta
    16801 aaattacaat caataaaaaa gatgtagaag agtacatccc acacgcagac cttcgtcttg
    16861 ttatcaacca acctttcgca gttacttcaa ctcaaggttc atacgatgtt tttgttaacg
    16921 ttgtaggtgg tggatatgca ggtcaatcag gtgcgatccg tcatggtatc tcacgtgcac
    16981 ttttagaagt tgacccagat ttccgcgatt cattgaaacg tgctggactt cttacacgtg
    17041 acgcacgtat ggttgaacgt aaaaaaccag gtcttaaaaa agcacgtaaa gcatcacaat
    17101 tttcaaaacg ttaatatact gttatatcaa cgtttcaaag cactttgcaa gcaattgcaa
    17161 ggtgtttttt tattgtcaat tttacttttg ataacctgat aaccccttcg cgaatatatt
    17221 gcaatacata gtacactttt tggatgtaaa ttgatgagta ttagc
//
LOCUS       DATRQA010000034        16560 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.034, whole genome shotgun sequence.
ACCESSION   DATRQA010000034 DATRQA010000000
VERSION     DATRQA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16560
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.034"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            44..1660
                     /gene="nikA"
                     /locus_tag="VBD27_000804"
     CDS             44..1660
                     /gene="nikA"
                     /locus_tag="VBD27_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HEO7024833.1"
                     /translation="MRRNILLSITCLLMVTLTACHSQDSKSHKLNSDKLTLAWGEDFG
                     DVNPHRYNPDQFVIQDMVYEGLVRYGDNGKIEPALAKSWSISQDGKTYTFKLRNAKYS
                     DGSNFNAANVKRNFDSIFSKSNRGNHNWFNLTNQLENYRALNQSTFEIKLKQAYSATL
                     YDLSMIRPIRFLSDSAFPKGDDTTKKNVKKPIGTGQWVVKSKKQNEYITFKRNENYWG
                     KKPKLKEVTVKVIPDAQTRALAFESGDVDLIYGNGIIGLDTFAQYTKDKKYVTAISQP
                     MSTRLLLLNAKESIFQDKKVRQAMNHAIDKVSIAKNTFRGTEKPADTIFSKSTSHSDA
                     KLNPYSYNVDKANQLLDQAGWKMGKDKVREKDGKALTLRLPYIATKATDKDLVTYFQG
                     EWRKIGINVSLIAMEEDDYWANAKKGNFDMMLTYSWGAPWDPHAWMSALTAKADHGHP
                     ENIALENLATKTEMDRLIKSALVDPKEENVDRDYKKVLELLHDEAVYIPLTYQSVISV
                     YRKGDFKTMRFAPEENSFPLRYIEKNNVSK"
     gene            1647..2591
                     /locus_tag="VBD27_000805"
     CDS             1647..2591
                     /locus_tag="VBD27_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7024834.1"
                     /translation="MYLSSSIIKKILSAFLALFFISLLTFILIKLSTVNPAENYLRLS
                     KISVSPEALKEAEHYLGLDKPLWKQYWLWFQKALTGDFGYSYVLRLPVLDLVLQRFLA
                     TLFLGTSAFLLIVTISTPLGVWAGLHESARSDHLIRFLSFSSVSMPNFWVAYLLMLLF
                     SAKLNLLPVSGGNDLQSLILPSITLSFSTVGQYIALIRKAISQENRSLNVENARLRGV
                     KERYIVTHHLLRNALPAIMTALSLTWVYLLTGSIIVEEIFSWNGIGRLFVTSLRTSDL
                     PVIQACMLIFGTLFLANNFMTQCFMNWVDPRLRKSREK"
     gene            2591..3397
                     /locus_tag="VBD27_000806"
     CDS             2591..3397
                     /locus_tag="VBD27_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7024835.1"
                     /translation="MSKRIYFSSSLLVLLVISAIFAPILSSFDPQYVDLSQKLLAPNN
                     VHLLGTDQLGRDVLSRLLYGARYSLFLAIIISLLELTIGMFVGLIVGWYQGKLENLFL
                     WIANIILAFPSFLLSLATVGILGHGLGNLIFAIVFVEWVYYAKLMTNLVKSAKKEPYV
                     INAQIMGLSVWHILRKHIFPFVYQPILVMVLMNIGNIILMISGFSFLGIGVQPNVTEW
                     GMMLHDARGYFRTATWMMLSPGIAIFLTVFSFNTLGDAIDKKDWKRQWNS"
     gene            3385..4173
                     /locus_tag="VBD27_000807"
     CDS             3385..4173
                     /locus_tag="VBD27_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7024836.1"
                     /translation="MEQLEIRKLSLQIGEVPVLRDFSCKIDMGESLTIIGESGSGKTL
                     LAKLLVGHIPQGMTVRGNIFFKGVDLGKLTVKQWQKLRGRDIAYLVQNPMSMFNPFQK
                     IEAHILETILSHEKCSKRVALSKALEWMKRLNLDDAISLLKKYPFELSGGMLQRIMLA
                     TILSLDPQVIILDEPTSAVDCHNCSTISAILQELQNNGKTLITVTHDYQLARDLGGQL
                     LVISEGEVVEQGQTQAILSNPQHNYTKALTVQMEYEGDILNVGL"
     gene            4160..4840
                     /locus_tag="VBD27_000808"
     CDS             4160..4840
                     /locus_tag="VBD27_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7024837.1"
                     /translation="MLVCNHVGKTFGRQEVLKDCHFHLKRGEIIGIMGKSGSGKSSLA
                     RLIIGLDSPTCGSIYFQGKIYTPKDGKAQIILVFQDALSSVNPYFSIEEILNEAFYGK
                     KTTFELCQILEAVGLDGTYLKYKARQLSGGQLQRVCIARALLLKPKIIIFDESLSGLD
                     PVTQIKMLRLLQKIKRRYELSFIMISHDPKICQAICNRVFLIKNGYLVEDNEFLKRAC
                     STNCLTNL"
     gene            4961..5689
                     /locus_tag="VBD27_000809"
     CDS             4961..5689
                     /locus_tag="VBD27_000809"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO7024838.1"
                     /translation="MEPKYQRILIKLSGEALAGDKGVGIDIPTVQSIAKEIAEVHNSG
                     VQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQYGVDTRVQT
                     AIPMQTVAEPYVRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEAEAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHVEVIKRGLKIMDATASTISMDNDIDLVVFNMN
                     ETGNIKRVVLGEQIGTTVSNKASE"
     gene            5705..6262
                     /gene="frr"
                     /locus_tag="VBD27_000810"
     CDS             5705..6262
                     /gene="frr"
                     /locus_tag="VBD27_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO7024839.1"
                     /translation="MTKEIVTKAQERFEQSHQSLSREFAGIRAGRANASLLDRIQVEY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERAINESDLGINPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKIAIRNIRRDAMDEAKKQEKNKEITEDDLKSLEKDI
                     QKATDDAVKHIDEMTANKEKELLEV"
     gene            6380..7234
                     /gene="cvfB"
                     /locus_tag="VBD27_000811"
     CDS             6380..7234
                     /gene="cvfB"
                     /locus_tag="VBD27_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEO7024840.1"
                     /translation="MNTLLATVITGLVTDENKDFYFIQKDGFTFALSKSEGEHHIGEM
                     VKGFAYTDMQQKARLTTKETFATRDHYGWGTVTEVRKDLGVFLDTGLPDKQVVVSLDV
                     LPELKELWPKKGDRLYVCLDVDKKDRLWALPADPEVFQRMATPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYSEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKKIKQ
                     DQLGTELL"
     gene            7372..7881
                     /gene="msrA"
                     /locus_tag="VBD27_000812"
     CDS             7372..7881
                     /gene="msrA"
                     /locus_tag="VBD27_000812"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO7024841.1"
                     /translation="MERAIFAGGCFWCMVQPFEELDGIESVLSGYTGGHVENPTYKEV
                     CSKTTGHTEAVEIIFNPEKISYADLVELYWAQTDPTDAFGQFEDRGDNYRPVIFYENE
                     EQRQIAQKSKDKLQASGRFDRPIVTSIEPADTFYPAEDYHQAFYRTNPARYALSSARR
                     HAFLEENWH"
     gene            7878..8093
                     /locus_tag="VBD27_000813"
     CDS             7878..8093
                     /locus_tag="VBD27_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEO7024842.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADYAFDETTFPKHSSDFETV
                     SRYLEDEASFSFNLTDFDDIWEDYLNH"
     gene            8250..10022
                     /locus_tag="VBD27_000814"
     CDS             8250..10022
                     /locus_tag="VBD27_000814"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEO7024843.1"
                     /translation="MRYTNGNFEAFARPRKPEGVDKKSAYIVGSGLAGLAAAVFLIRD
                     GQMDGQRIHIFEELPLSGGSLDGVKRPDIGFVTRGGREMENHFECMWDMYRSIPSLEV
                     PDASYLDEFYWLDKDDPNSSNCRLIHKQGNRLESDGDFTLGTHSKELVKLVMETEESL
                     GAKTIEEVFSKEFFESNFWTYWATMFAFEKWHSAIEMRRYAMRFIHHIGGLPDFTSLK
                     FNKYNQYDSMVKPIISYLESHNVDVQFDSKVTNISVDFKNGQKLAKAIHLTVGGEAKT
                     IDLTPNDFVFVTNGSITESTNYGSHDTVAKPNTDLGGSWNLWENLAAQSDEFGHPKVF
                     YKDIPKESWFVSATATIKDPAIEPYIERLTHRDLHDGKVNTGGIVTVTDSNWMMSFAI
                     HRQPHFKEQKENETIVWIYGLYSNVEGNYIKKPIEECTGREITEEWLYHLGVPEMKIH
                     DLSDKQYVSTVPVYMPYITSYFMPRVKGDRPDVIPQGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYTFLNIERGVPEVFNSAFDIRVLLQSLYYLNDKKSVEDMDLPIPAL
                     MRKVGMKKIRGTYLEELLREAHLL"
     gene            10146..11132
                     /locus_tag="VBD27_000815"
     CDS             10146..11132
                     /locus_tag="VBD27_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO7024844.1"
                     /translation="MQEYSIEITLQHPDDMMSLFGSNERHLKLIEENLDVIIHARTER
                     VQVLGDSEEAVETARLTIEALLVLVNRGMTVNTSDVVTALSMAQNGSIDKFVALYEEE
                     IIKDSYGKPIRVKTLGQKIYVDSVKNHDVVFGIGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTSRLMEREIIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDVSQIDLPKNVKS
                     GLIDAVEKLRNIKKIDFIHLSAKDVVRHPVVAEIINAYSDSESSHKLQSDKD"
     gene            complement(11223..12026)
                     /locus_tag="VBD27_000816"
     CDS             complement(11223..12026)
                     /locus_tag="VBD27_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEO7024845.1"
                     /translation="MDHFTKLWQDFSKLPNVVAIALGGSRSGDSFDQSSDYDLYVYCA
                     ATPDITSRKRILNKHCHYIELNNHYWELEDNGTLNDGTDIDILYRNIDNFLSDLEDVV
                     EHHNSRIGYTTCFWHNLINCQILYDPENQLQSLKERFEVSYPSQLQKQIIIQNRNLLT
                     GKLPSYDKQIIKALKRQDFVSTHHRTTAFLDSYFDIIFALNKLTHPGEKRMISYAKKN
                     ATLLPKHFEENIIKLCHHNSNEHTVKETLNDIIMHLDVMLKENFQHFIG"
     gene            12218..12694
                     /locus_tag="VBD27_000817"
     CDS             12218..12694
                     /locus_tag="VBD27_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO7024846.1"
                     /translation="MKQDYISYIRSKVGHETIFLTYSGGILTDGKGRVLLQLRADKNS
                     WGIIGGRMELGESSVDTLKREFFEETGLRVEPIRLLNVYTNFQDSYPNGDKAQTVGFI
                     YEVSCPKPVNIEGFHNEETLQLDYFSKEDVKNITIVNEQHQLILDEYFSQTFQMGR"
     gene            13016..13822
                     /locus_tag="VBD27_000818"
     CDS             13016..13822
                     /locus_tag="VBD27_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7024847.1"
                     /translation="MKITLHGVAETLLITLYIRAKDAMAKHPILNDQKSLAIVEQIEY
                     DFDKFDNSEASFYATLARIRVMDREIKKFIRENPNSQILSIGCGLDTRFERVDNGQIR
                     WYNLDLPEVMEIRKLFFEEHERVTNIAKSALDETWTREVNPQNAPFLIVSEGVLMFLK
                     EDDVETFLHILTNSFSQFMAQFDLCHKEMINKGKQHDTVKYMDTEFQFGITDGHEIVD
                     LDPKLKQINLINFTDEMSKFELGTLRSLLPTIRKFNNCLGVYEYKASEKK"
     gene            14217..14714
                     /gene="ybeY"
                     /locus_tag="VBD27_000819"
     CDS             14217..14714
                     /gene="ybeY"
                     /locus_tag="VBD27_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO7024848.1"
                     /translation="MYVEMIDETGQVSEDIKKQTLDLLEFAAQKTGKENKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPEVDISFDEEDLAENPELAEMLEDFDSYIGEL
                     FISIDKAKEQAEEYGHSYEREMGFLAVHGFLHINGYDHYTPEEEKEMFSLQEEILTAY
                     GLKRQ"
     gene            14695..15093
                     /locus_tag="VBD27_000820"
     CDS             14695..15093
                     /locus_tag="VBD27_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEO7024849.1"
                     /translation="MDLNDNNQKKWKNRTLTSSMEFAVTGIFTAFKEERNMRKHLVSA
                     ILVILAGLTFQVSMVEWLFLLLSIFLVITFEIINSAIENVVDLASNYHFSMLAKNAKD
                     MAAGAVLVVSLFAVLVGLIIFIPKILALLL"
     gene            15135..16034
                     /gene="era"
                     /locus_tag="VBD27_000821"
     CDS             15135..16034
                     /gene="era"
                     /locus_tag="VBD27_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO7024850.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGD
                     DMIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALQGNNVPT
                     LIKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            16271..>16560
                     /locus_tag="VBD27_000822"
     CDS             16271..>16560
                     /locus_tag="VBD27_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024851.1"
                     /translation="MHNILRFLGIVIISAVILFSIGSFYDLSLMKNILLICWSFLFDL
                     LVFVFKQRQTTEVLTWYQVVKQFWLFIKCTILIPILVAFIIMKGCLTSISDI"
BASE COUNT         5500 a         2411 c         3302 g         5347 t
ORIGIN      
        1 cttaaaaaaa taattaacta aacttaaaag aaagttggta aaaatgagac gtaacatatt
       61 attgagcatt acttgtttat tgatggtgac tctgactgca tgccattcac aggatagcaa
      121 aagtcataaa ctaaacagtg ataaactcac gcttgcttgg ggagaagact ttggtgatgt
      181 taatccgcat cgctataacc ctgatcagtt tgttatccag gatatggttt atgaggggtt
      241 ggtacgatat ggtgacaatg ggaaaattga accagcttta gcgaaaagct ggagcattag
      301 tcaagatgga aaaacctaca cttttaaatt gagaaatgct aaatattctg atggcagtaa
      361 ctttaatgct gcgaatgtca aacgtaattt tgatagtatt ttttcgaagt ctaatagagg
      421 taatcataat tggtttaatt taacgaatca attggaaaat tatcgtgcct tgaatcaaag
      481 tacttttgaa attaaactga aacaagctta tagtgctact ttatatgatc tctctatgat
      541 tcgaccaatt cgctttttat ctgatagcgc tttccctaaa ggtgatgata caactaagaa
      601 gaatgtaaaa aaaccaattg gaacgggtca atgggttgtg aaaagtaaaa agcaaaatga
      661 atatattacc ttcaaacgta atgagaatta ctggggaaag aaaccaaaat taaaagaagt
      721 aaccgtaaaa gttatacccg atgctcagac gcgtgcttta gcattcgaat caggtgacgt
      781 tgatttgatc tatgggaatg gcattattgg tttagacact tttgcccaat atacgaagga
      841 caagaaatat gtcacagcta tttctcaacc tatgtcaaca agacttttgt tgctaaatgc
      901 taaggaaagt attttccaag ataaaaaagt tcgtcaggca atgaatcatg ctattgacaa
      961 ggtttctatt gctaagaaca cctttagagg aacagaaaag ccagcagaca ctattttttc
     1021 aaaatcaaca tctcactcag atgctaagtt aaatccttat agctataatg ttgataaagc
     1081 aaatcagcta ttggatcaag ctggttggaa gatgggaaaa gataaggttc gtgaaaaaga
     1141 tggtaaagct ctaaccttac gtcttcctta tatcgccact aaggcaactg ataaagacct
     1201 ggtaacttat ttccaaggag aatggcgtaa aatcggtatt aatgtttctc ttattgctat
     1261 ggaagaagat gattattggg ctaatgctaa aaagggtaat tttgatatga tgttaaccta
     1321 ttcttgggga gcaccatggg accctcatgc ttggatgtcg gcattaactg ccaaggcaga
     1381 tcatggacat ccagaaaata tagctttaga aaatctagca actaaaactg aaatggatag
     1441 acttattaag tcagctctag tagatcctaa ggaagaaaat gttgatagag actataagaa
     1501 ggttcttgaa ttattgcatg atgaggctgt atatatccca ttaacttatc aatcggtgat
     1561 ttctgtttat aggaaaggtg attttaaaac catgcgtttt gcgccagaag aaaattcatt
     1621 cccattacgc tatatagaaa aaaacaatgt atctaagtag ttcgatcatc aaaaaaatat
     1681 tgtcagcttt tttggcattg tttttcatat cacttctaac atttattctt atcaaactat
     1741 caacagttaa tccagcagaa aattaccttc gtttatcaaa aataagtgtt agtccagaag
     1801 cgctaaaaga agcagagcac tatttaggtt tagataagcc attatggaag cagtactggc
     1861 tgtggttcca aaaagcatta acaggagatt ttggatattc ttatgtgctc aggttgccag
     1921 tattagattt ggttttacaa cggtttttgg ctactttatt tttaggcacg agtgccttct
     1981 tgctaattgt taccatttct accccgttag gtgtgtgggc tggcttacat gaatctgctc
     2041 ggagtgatca cttgattcga tttttgagtt tttctagtgt gtctatgcca aatttttggg
     2101 ttgcttactt gttgatgctg ctattttcgg caaaattaaa tcttctgcct gtttcaggag
     2161 gtaacgattt acaaagttta attttgccaa gtattacctt aagtttttct accgtaggtc
     2221 aatacatagc ccttattcga aaagctatta gtcaagaaaa tagaagttta aatgtggaaa
     2281 atgcccgatt aagaggagta aaagaacgtt atattgtcac acatcacctc cttagaaatg
     2341 ccttacctgc gataatgaca gcacttagct tgacttgggt ttacttatta acaggatcaa
     2401 ttattgttga agaaattttt tcatggaatg gaataggacg tttatttgtg accagtctaa
     2461 gaacttcaga tcttccagta atacaagctt gtatgctaat ttttggaacc ttatttttag
     2521 ctaataattt tatgacacag tgttttatga attgggttga tcctcggtta cgaaagtcaa
     2581 gggagaaata atatcaaaaa gaatttattt ttcgagtagc ttactggtat tgttagtgat
     2641 tagtgccatt tttgccccta ttctatctag ctttgatcca caatacgtag atttatcaca
     2701 aaaattattg gctcccaata atgttcattt gttagggact gaccaattgg gtagagatgt
     2761 attatctcgc ttgctttatg gtgctagata ttcactgttt ttagctatta ttattagctt
     2821 attggaatta acgattggta tgtttgtggg tcttattgtt ggttggtatc aaggaaagtt
     2881 agaaaacctc tttttatgga tagctaatat tattttagct tttccgagct ttttgctctc
     2941 tcttgcaacc gtggggatct taggtcatgg tctagggaat ttaatttttg caattgtctt
     3001 tgtggaatgg gtttactatg ctaaattaat gaccaattta gttaagagtg caaaaaaaga
     3061 gccttatgtg ataaatgcgc aaattatggg actttcagtc tggcatattt taagaaaaca
     3121 tatttttcct tttgtgtatc agccaatcct cgttatggtc cttatgaata taggaaatat
     3181 tattttaatg atttctggct tttctttctt agggattggt gtgcaaccta atgtcacaga
     3241 atggggaatg atgttgcacg atgctagagg atattttagg acagctactt ggatgatgtt
     3301 atctcctgga attgcgattt ttttaacagt attttccttt aatactttag gcgatgccat
     3361 agataaaaaa gattggaaac gacaatggaa cagttagaaa taagaaaatt gtcattacag
     3421 ataggagagg tccctgtact aagagatttt agttgtaaaa tagatatggg agaatcgcta
     3481 actattattg gtgagagtgg ttcagggaaa accctcttgg caaaattatt ggttggtcac
     3541 attccacaag gtatgacagt tagaggaaac atatttttta agggagttga tttaggtaaa
     3601 ctaactgtaa agcagtggca gaaattaagg ggacgagata ttgcctattt agtgcaaaat
     3661 cccatgtcta tgtttaatcc ttttcaaaaa attgaagcgc atattttgga aacaatcctt
     3721 agtcatgaga aatgttcaaa gagagtagcc ttatctaaag cgttagaatg gatgaaacgt
     3781 ttaaatttag atgatgcgat atccctgtta aaaaaatacc cttttgagct tagtggagga
     3841 atgctacaaa gaattatgtt agccactata ttatctcttg atcctcaggt aatcatatta
     3901 gatgagccga cctcagcggt tgattgtcat aattgttcta ctatatcagc tattttgcag
     3961 gaattgcaaa ataatggaaa aaccttgatt actgtaacgc atgattatca gttagctaga
     4021 gacctggggg gacaattatt agttataagt gagggagaag ttgttgaaca gggacaaacc
     4081 caagctattt taagcaatcc tcaacataac tatacgaaag cactgacagt gcaaatggag
     4141 tatgaaggag atattcttaa tgttggtttg taatcatgtc ggaaaaacat ttggacgaca
     4201 agaggtttta aaggactgtc attttcatct taaaagagga gaaataattg gtataatggg
     4261 taaaagtggc agtggtaaaa gtagccttgc ccgactcatt ataggactag atagtcctac
     4321 ttgtggttcg atatattttc aaggaaaaat ttacacacct aaagatggta aggcgcagat
     4381 tatccttgtt tttcaagatg cgcttagttc ggtcaatcca tattttagta ttgaggaaat
     4441 tttgaatgaa gctttttatg gaaaaaaaac aacttttgaa ttatgtcaaa tattagaagc
     4501 ggtgggttta gatggaactt atctaaaata taaagctaga caacttagtg gtggtcagct
     4561 acagagagtt tgtatcgcta gagctttact cttaaagcct aaaattatta tttttgatga
     4621 atccttaagt ggattagacc cagtaactca aattaaaatg ctacgtttat tacaaaaaat
     4681 aaaacgccgt tatgaattaa gttttataat gatttctcat gaccctaaaa tttgtcaagc
     4741 tatctgtaac cgggtctttc tgataaaaaa tggttattta gtagaagata atgaattctt
     4801 gaagagagcg tgttctacta actgtttgac caatctctga attattatgt ttttcttatt
     4861 ttaggagttg ataaagaaat caacttacta gtgtcattgc tctattaaaa ttttgccttt
     4921 tatggtaaaa taataaagac aaaataagga gaacatagta gtggaaccta aatatcaacg
     4981 tatattaatt aagttatctg gtgaagcatt ggcgggagat aagggtgtcg gtattgacat
     5041 tcctaccgtt caatctattg caaaagaaat tgccgaagta cacaattcag gtgttcaaat
     5101 agcacttgtc attggtggag gtaatctttg gcgtggagaa cctgcagcag aagcagggat
     5161 ggatcgtgtt caagctgatt atacagggat gttagggaca gtaatgaatg ctcttgtaat
     5221 ggctgatagc cttcaacaat atggggtaga tactagagtt caaacagcta ttccaatgca
     5281 aactgttgct gagccttacg tacgtggtcg tgctttacgt catctagaga agaatcgtat
     5341 tgttgtcttt ggagcgggta ttggttcgcc ttatttctca actgatacaa cagcagcgct
     5401 tcgtgctgct gagattgagg cagaagcaat tttaatggct aaaaatggag tagatggtgt
     5461 ttataatgct gatccaaaga aagatgccaa tgctgtcaaa tttgatgaat taactcacgt
     5521 tgaagtaata aaacgagggt taaaaatcat ggatgcaacc gcatcaacta tttcaatgga
     5581 taatgatatt gaccttgttg ttttcaatat gaatgagact ggtaatatta agcgtgttgt
     5641 tcttggagaa caaatcggaa caactgtttc aaacaaagca tctgaatagg agtgtataaa
     5701 aaatatgact aaggaaatcg ttacaaaagc tcaagagcgt tttgagcaat cacaccaaag
     5761 tttatcaaga gaatttgcag ggattcgtgc aggacgtgct aatgctagct tgcttgatcg
     5821 tattcaagtt gagtattatg gagcgccaac cccacttaac caattagctt ctattactgt
     5881 acctgaagct cgtgttcttt taatttcacc atttgataaa tcatcaatca aagatattga
     5941 gcgtgctatt aacgaatcag atttaggtat taatcctgca aatgatggtt cagttattcg
     6001 tcttgttatt ccagctttga cagaagaaac acgtcgtgat ttagctaaag aagtgaaaaa
     6061 agtcggagaa aatgctaaga ttgctatccg taatattcgc cgtgatgcta tggatgaagc
     6121 taagaagcaa gaaaagaata aagaaattac agaagatgac ttgaaatctc ttgaaaaaga
     6181 tattcaaaag gctactgatg atgctgtaaa acatattgat gagatgactg ctaataaaga
     6241 aaaagaattg ttggaagttt aatagtaacg tttgctatct ttattatgat tggcaagtaa
     6301 taaaatgatt atggaggtaa ctgatagaat caaaaccaga tggatttggt ttgtcggtta
     6361 cctttttaaa aggagaaaaa tgaatacatt attagcgaca gtcataacag gcttagtaac
     6421 tgatgaaaac aaagattttt actttatcca aaaagatggc tttacctttg cattatcaaa
     6481 gtcagaagga gagcatcaca ttggtgaaat ggttaaagga ttcgcctata cagatatgca
     6541 acaaaaagca cgtcttacaa ctaaagaaac atttgcgact cgagatcatt atggttgggg
     6601 gacagttact gaggtacgta aagacttggg agtatttctt gatactggtc ttccagataa
     6661 gcaagtggtt gtttctttgg atgttcttcc tgaattgaaa gaactttggc ctaaaaaagg
     6721 tgatcgcctc tacgtttgct tagatgttga taaaaaagat cgtctctggg ctctccctgc
     6781 agatcctgaa gtgtttcaac gaatggcaac ccctgcatat aacaatatgc aaaatcaaaa
     6841 ttggccagca atcgtttatc gcttaaaatt gtcaggaact tttgtttatc taccagaaaa
     6901 taatatgctc ggctttattc accctagtga acgttacagt gaacctcgtt tagggcaagt
     6961 gctggatgca cgagttattg gcttcagaga agttgataga accttaaatt tatcattaaa
     7021 gcctcgttct tttgaaatgt tagaaaatga cgcacaaatg attttaacgt atttagaaag
     7081 taacggagga ttcatgactt taaatgataa atcttctcca gaggaaatta aagctacttt
     7141 tggaatttcc aaaggtcagt ttaaaaaagc acttggagga ctcatgaaag ctaaaaagat
     7201 caaacaagat cagctaggta cagaattatt ataaactttt gaacttttaa agttttctca
     7261 cgaggtcgtt ttagttatag ctagaattaa tggctaatta tagaaaaaag ctgagaggaa
     7321 attttggctt tttttgttat aatgtatcta ttaaaaagaa tggagaacga tatggaacgt
     7381 gcgatttttg ctggaggttg tttttggtgt atggtacaac catttgaaga attagatggt
     7441 attgaatctg ttttatctgg atacactgga ggacatgttg agaatccaac ttataaagaa
     7501 gtttgtagta aaactactgg acatacagaa gctgttgaaa ttatttttaa tcctgaaaaa
     7561 atctcttatg cagatttagt tgagttatat tgggcacaaa cagatccaac agacgctttt
     7621 ggtcagtttg aagatcgtgg ggataattat cgtcctgtca tcttttatga gaatgaagag
     7681 caacgtcaga ttgctcaaaa atcaaaagat aagctacaag cttcgggtag atttgataga
     7741 ccaattgtaa ccagcattga gcctgctgat actttttacc cagcagaaga ctatcatcaa
     7801 gcattctatc ggactaaccc tgctcgttac gccttgagta gtgccagaag acatgcattt
     7861 ctagaggaga actggcattg agaaaatcat tttacagttg gttgatgaca caaagaaatc
     7921 caaaatcaaa tgaaccagta gcaatcctag cagattacgc ttttgatgaa accacttttc
     7981 caaaacatag ttcggatttt gaaacagtta gtcgctattt agaagatgag gccagttttt
     8041 cctttaattt aacagatttt gatgatattt gggaagatta tctcaatcat taacaataat
     8101 atagagattg tattttcaat ctctttttta ggttatagaa attggacaca ttgtctaaga
     8161 ctgtctgaaa agcaggtttt ttaatggcaa tcgttttcac aaaggagtag aatatagtca
     8221 tctaaaatat agccctaggg aggttgttta tgagatatac aaatggaaat tttgaagcct
     8281 ttgcaagacc tcgaaaacct gaaggtgtgg ataaaaaatc cgcttatatt gttggttctg
     8341 gtttagcagg attagctgcc gctgtctttt taatacgtga cggtcaaatg gatggtcaac
     8401 gtattcatat ttttgaagaa ctacctcttt ctggaggatc acttgacggt gtcaaacgac
     8461 ctgatatcgg ttttgtaacg cgtggtggtc gtgaaatgga aaatcacttc gaatgtatgt
     8521 gggatatgta ccgttccatc ccctctctcg aagttccaga tgcttcttat ctagatgaat
     8581 tttattggct tgacaaggat gatcccaatt catctaactg tcgcctcatt cataaacagg
     8641 ggaatcgctt agaatctgat ggtgatttta cactcggaac acattccaaa gagttagtta
     8701 agctagtcat ggagactgaa gagtctttag gtgctaagac gattgaagaa gttttttcaa
     8761 aagaattttt tgaaagtaat ttttggactt attgggctac tatgtttgcc tttgagaaat
     8821 ggcattcagc gattgaaatg cgtcgatatg ctatgcgctt tatccatcat attggtggtc
     8881 tgcctgattt cacttcatta aaatttaata aatataatca atatgattct atggtgaaac
     8941 caatcatcag ttatttagag tctcataatg tagatgttca atttgatagc aaggtaacta
     9001 atatctccgt agactttaag aacggtcaga agcttgcaaa agccattcat ctcacagttg
     9061 gtggtgaagc aaaaacaatt gatcttactc caaatgattt tgtttttgta acgaatggtt
     9121 ctatcactga aagtacgaac tatggtagtc atgatactgt agctaaacct aataccgatt
     9181 tgggaggttc ttggaatctt tgggaaaact tagctgctca atctgatgaa tttggacatc
     9241 ctaaagtctt ctacaaagat attccaaaag agtcatggtt tgtttctgct actgcaacaa
     9301 taaaagatcc agctattgaa ccttatatag agcgtttaac acatcgtgac ctccatgatg
     9361 gtaaagtaaa taccggaggc attgttacag ttacagattc taattggatg atgagttttg
     9421 ctatccatcg ccaaccgcat ttcaaagagc aaaaggaaaa tgaaacaatt gtttggattt
     9481 atggactcta ttcaaatgtg gaaggtaatt atattaaaaa accaattgaa gaatgtactg
     9541 gtcgagaaat cacagaagag tggttatatc acttaggtgt tcctgaaatg aagattcatg
     9601 atttatctga taaacaatat gtaagtacag ttcctgttta tatgccttac attactagtt
     9661 atttcatgcc tcgagttaaa ggtgaccgac cagatgttat cccacaagga tctgttaacc
     9721 tagccttcat tggtaatttt gcagaatctc catccagaga tacagtattt acaacggaat
     9781 attctattag aactgctatg gaagctgttt atactttctt aaatattgaa cgtggtgttc
     9841 cagaagtctt taattcagca tttgatattc gtgtcttgtt gcaatcttta tactatctca
     9901 atgataagaa atctgttgaa gatatggatt taccaatacc tgccttaatg cgtaaagtag
     9961 gcatgaaaaa aattagagga acttatttag aagaattact tcgagaagct catcttttat
    10021 aatgtaaaat aaaatcgaga aaacttgttt ttctcgattt tttagtttta caacgactaa
    10081 aatcaccaaa gaaaatgaaa ttttgttata atgtagtcac ttactgtacg aaaaggaaga
    10141 ataatttgca agaatactct atcgaaatta ctttacaaca tccagatgat atgatgtcac
    10201 tctttggcag caatgagcgc catttaaaat taatcgaaga aaatttagat gttatcattc
    10261 atgctagaac tgaacgtgtt caggtattag gtgactctga agaagcagta gaaaccgctc
    10321 ggttaaccat tgaagcatta ttagtgcttg ttaaccgcgg gatgactgtg aatacttcag
    10381 atgttgtaac agccttatcc atggcacaaa atggttcaat tgataaattc gttgcccttt
    10441 atgaggaaga aattattaag gatagctatg gtaagcctat ccgtgttaag actttagggc
    10501 aaaaaatata cgttgatagc gttaaaaatc atgatgttgt ttttggtatt gggcctgctg
    10561 gaacgggaaa aactttttta gcagtcactc tagcagtaac ggcactaaag cgtggtcaag
    10621 ttaaacgaat aatcttaacg agaccagcgg ttgaagctgg tgaaagcctt ggtttcttgc
    10681 ctggagatct caaagagaaa gttgatcctt atttgagacc agtttatgat gccctatatc
    10741 aaattttagg taaagagcaa acaagtcgct taatggagcg tgaaatcatc gaaattgctc
    10801 cattggctta catgcgtggt cgtactttag atgatgcatt tgtcatatta gatgaagcgc
    10861 aaaacaccac tataatgcaa atgaaaatgt tcctaacacg tcttggcttc aattctaaaa
    10921 tgattgtaaa tggcgatgtt agccaaattg acttaccaaa gaatgttaaa tctggtttga
    10981 ttgatgctgt tgagaaatta cgaaatatca aaaagattga ctttattcat ttatcagcaa
    11041 aggatgttgt aagacaccct gtcgttgctg aaataataaa tgcttattca gattcagaaa
    11101 gtagtcacaa gctacaatct gataaggact gatgtttgcc actaggagac gttgttagag
    11161 agtgtgtcaa tatgtaaaaa acgcctaatg ctttaggcgt ttttatgatg gtgtacttca
    11221 acttaaccta taaagtgttg aaaattttct ttaagcatga cgtcgagatg cattattata
    11281 tcgtttaatg tttctttaac agtgtgttcg ttggagttgt gatgacatag tttaatgata
    11341 ttttcttcga aatgtttagg aagcaatgta gcattcttct tagcatagga aatcattctt
    11401 ttttcgccag gatgtgtcaa cttattaagt gcaaaaataa tatcaaagta ggaatctaag
    11461 aaagcagtag ttctatggtg agtactaaca aagtcttggc gtttaagggc ttttataatt
    11521 tgtttatcgt aagagggaag cttgccagtt aataagttac gattttgaat gataatttgt
    11581 ttttgtaact gactgggata agaaacttcg aatctctctt tgagtgattg taattgattt
    11641 tcaggatcat agagtatttg gcaattgatg aggttatgcc aaaaacaagt agtgtatcca
    11701 attcgagaat tgtggtgttc aacgacatct tctaagtctg ataaaaagtt atctatgtta
    11761 cgatagagaa tatcaatatc agttccgtcg tttaaagtac cattatcttc aagctcccag
    11821 taatgattgt taagttcaat gtaatgacag tgcttgttaa ggatacgttt acgactagtg
    11881 atatcaggag ttgctgcaca ataaacatac aaatcataat cggaagattg gtcaaaactg
    11941 tcaccagagc gtgagcctcc gagtgctata gcaacaacat ttggaagttt agagaaatct
    12001 tgccagagtt ttgtgaaatg gtccataaat gctctcctaa aaatgtaaaa tattgatgtg
    12061 tctccattct acagggctat ctctttttag tcaataatta atagtaagtt aataaaagag
    12121 gtaacggttc taatattcaa gaagtgtata tgagtgattt gaaaaggaga tattttatgc
    12181 tacaatacgt acatattagt ttaaacaagg tgatagaatg aaacaagatt atatttccta
    12241 cattaggtca aaagttggac atgagactat ctttttaaca tattctggtg gtattttgac
    12301 agatggtaag ggacgtgtct tactccaatt aagagctgat aagaattctt ggggaattat
    12361 tggtggacgt atggagttag gtgagtcatc agtagataca ttgaaaagag aatttttcga
    12421 agaaactgga ttgagagtgg aaccaatcag actgttaaat gtttatacta actttcaaga
    12481 ctcttatccc aatggcgata aagcacaaac cgtcggtttt atttatgaag taagctgtcc
    12541 aaaaccagtt aatatagaag gttttcataa tgaggaaaca ttgcaattgg actatttttc
    12601 taaagaagac gtcaaaaata ttacgatcgt aaatgaacaa caccaattaa ttttagatga
    12661 atatttttca caaacattcc aaatggggcg ttaaaaggat gggaaatact aggtgtgact
    12721 ttaaagaact tctaactttt attgatatgt tgttaggcat tcttgtagaa acaaatgtta
    12781 agaaaataag gagttctcat ttgtatattt tataattatt aaaaagaaaa acagtagctt
    12841 gcaataattt agtgactgtt tttttagaaa gcataagaag tacagaaaaa gaaaattatg
    12901 gaatattgga tggtggggat atgctttact tacggtattg gaatgatata cttaaagaaa
    12961 tccagtttta gaaatccaaa tttctaagta aaatagcata aaggagagta gatgaatgaa
    13021 gataacatta cacggcgttg ctgaaacgtt attaatcacc ttatatatta gggctaaaga
    13081 tgcgatggct aaacatccga tacttaatga tcaaaaatcc ttagcaattg ttgaacagat
    13141 agaatatgat tttgataaat tcgataattc agaagcttct ttttatgcaa cattagctag
    13201 aattcgcgtt atggatagag aaatcaaaaa atttattaga gaaaatccaa atagtcaaat
    13261 cctttcaatt ggttgtggac ttgatacaag gtttgaaaga gtcgataatg gacaaattag
    13321 gtggtataac cttgatttgc cagaggttat ggagataaga aaattatttt ttgaagagca
    13381 tgaaagagtt actaatatag caaaatcagc cctagatgaa acttggacac gggaggtaaa
    13441 tccccaaaat gccccttttc taatcgtgtc agaaggtgtt ttaatgtttc taaaagaaga
    13501 tgacgtagag acttttcttc atatcctgac aaattcattt agccaattta tggcacaatt
    13561 tgatttgtgt cataaggaaa tgattaataa aggaaagcaa catgatacag taaagtatat
    13621 ggatacagaa tttcagtttg gtatcacaga tggtcatgag attgtggatt tagaccctaa
    13681 attaaagcaa ataaatctga ttaactttac agatgagatg agcaaatttg agttaggcac
    13741 acttcgctct ttacttccaa caattcgtaa atttaataat tgtttaggtg tgtacgaata
    13801 taaagcatct gagaaaaagt aacaaaaatc tatttatata aagagacatt ctaaatgtaa
    13861 atttgtcata atcgttatag catttgtagc tttgtttatt taggaattag ttttacgtaa
    13921 gttaggcgct ctaaagtttg tattttgaag taaatactaa ttcataagtc agaattttat
    13981 tgaaatggat tataagtgtg taatagtgat tattagcttt ctctcccttt tttaatactc
    14041 ttatggtata atgatgtgaa agaatagctt gaaggaagga agagaacgca cttttatttt
    14101 gagaactagt tttagataaa agtgaaaaac ttaggaaagt agatgtgtcg attatttaga
    14161 attatgcatt acactttatc ttttctaaac gttttgggtc gttcttacta tataagatgt
    14221 acgttgaaat gattgatgaa actggacaag tatcagaaga tatcaaaaag caaactcttg
    14281 atttgttaga gtttgcagca caaaaaacag gtaaagagaa taaagaaatg gctgtaacgt
    14341 ttgtcactaa tgaacgtagc catgaattga atttagagta tcgagatacc gatcgtccga
    14401 ctgatgttat cagtttagaa tataagcctg aggttgatat ttcttttgat gaagaagatc
    14461 tggctgaaaa tcctgaactt gctgagatgc tagaagattt tgattcttat attggtgaac
    14521 tttttatttc tattgataaa gctaaagaac aagcagaaga gtacggtcac tcttatgaac
    14581 gtgaaatggg ttttttagct gtacatggtt ttttgcatat taacggttat gatcattata
    14641 cacccgaaga agaaaaagag atgttcagct tacaggaaga aattttaact gcttatggac
    14701 ttaaacgaca ataatcaaaa aaaatggaaa aatcgcacct taacatcaag catggaattt
    14761 gctgttacag ggatttttac agcttttaaa gaagaaagaa atatgcgtaa gcatttggtt
    14821 tctgccattt tagtaatact tgctggtctt acttttcaag tatctatggt ggagtggtta
    14881 tttcttttat tgagtatttt tttggtaatt acttttgaaa ttatcaattc agctattgaa
    14941 aatgtagttg atttggcaag taattaccat ttctcgatgc tggctaaaaa tgctaaagat
    15001 atggcagcag gagcggtttt agttgtttcc ttatttgccg tattggtggg attgattatt
    15061 tttataccca agatacttgc tttactttta tgatgagaag tattacgaat aaattgaatg
    15121 gagaaagaaa agaaatgaca tttaagtcag gatttgtagc aattttaggt cgtccaaatg
    15181 ttggaaaatc aaccttccta aaccatgtta tgggacaaaa aattgctatc atgagtgata
    15241 aagctcaaac aacacgtaat aaaattatgg ggatttatac aacagaaact gagcaaattg
    15301 ttttcattga taccccagga attcataaac caaagaccgc ccttggtgat tttatggttg
    15361 aatctgctta tagtacttta cgtgaagttg aaacagttct tttcatggtt cctgccgatg
    15421 aaaagcgagg caaaggcgat gatatgatta ttgaacgtct taaggctgcc aagattcctg
    15481 ttattttagt aatcaacaaa attgataagg ttcatccaga ccaacttttg gaacagattg
    15541 atgacttccg tagtcaaatg gattttaaag aagtagtgcc aatttctgcc cttcaaggaa
    15601 ataatgtgcc aaccctaatc aagttattga ctgataatct tgaagaaggt ttccaatatt
    15661 tcccagaaga tcagattact gatcatccgg aacgattttt agtatcagaa atggttcgtg
    15721 agaaggtact tcatttaacg caacaagagg ttcctcactc agtagctgtt gttgtcgaat
    15781 ccatgaaacg tgatgaagaa actgataaag tgcatattcg tgcaactatc atggttgaac
    15841 gtgatagtca aaaaggcatt atcattggaa aacaaggtgc tatgctcaag aaaataggaa
    15901 aaatggcgcg ccgtgatatt gaacttatgt taggtgataa agtttaccta gagacttggg
    15961 taaaagtcaa gaaaaactgg cgtgataaaa agcttgattt agctgatttt ggctataatg
    16021 aaaaagagta ttaatgagtt tattttgaga tccgcaagtt agtggggaag agttagataa
    16081 gttcaccgtg agacttgatt cgcacagaaa aagcaattca cgaggataaa accaactagt
    16141 cataattttg agattatatt cacaaattaa ttaaagggag ccaaacatgc ttgttggccc
    16201 cttcttaaat aaaggagagc acatgactca agattatatt tgctacctat aaagtagagg
    16261 aggtgaggat atgcataata tattaagatt tttaggcatt gttattattt ctgcagtgat
    16321 tctatttagt ataggtagtt tctacgattt atcattaatg aaaaatatac tacttatctg
    16381 ttggagtttt ctctttgatc tattggtttt tgtcttcaaa caaagacaaa cgacagaagt
    16441 actcacatgg tatcaagttg ttaagcaatt ttggttattt attaagtgta ctattcttat
    16501 tcctatctta gttgctttta ttataatgaa aggatgtttg acaagtatat cagatatatt
//
LOCUS       DATRQA010000035        16095 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.035, whole genome shotgun sequence.
ACCESSION   DATRQA010000035 DATRQA010000000
VERSION     DATRQA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16095
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.035"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            306..1001
                     /locus_tag="VBD27_000823"
     CDS             306..1001
                     /locus_tag="VBD27_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7024852.1"
                     /translation="MFLIVPIFCIGYMWIAIENRNSEEQREKIAEQNQYINILSAENE
                     RNRIGRDLHDSLGHTFAMMTLKTELALKLLEKRNYDKVQKELSELNHISHQSMSEVRQ
                     IVSNLKYRTVVEEIDELYRLFQLSNIKLTVVNKLETSQLSPVTQSTITMILKELSNNI
                     VKHAEADSVELSLVRQGATINIEMIDNGCGFTNLDGDELHSIQERLTIVEGTLTILSR
                     SKPTHIQVILKEV"
     gene            1003..1593
                     /locus_tag="VBD27_000824"
     CDS             1003..1593
                     /locus_tag="VBD27_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7024853.1"
                     /translation="MKLLVAEDQSMLRDAMCQLLLMEESVSTIDQAGNGEEAIAILSN
                     KAIDVAILDVEMPILSGLDVLEWVRKYQNVKVIIVTTFKRSGYFQRAIRSNVDAYVLK
                     DRSVADLMKTIQKVLSGGKEYSPELMENVISNPLSKQEIKILSLIAQGKTSKEIACTL
                     FLSNGTVRNYTSNIFDKLGVNNRLEALKIAREKDWL"
     gene            1643..2347
                     /locus_tag="VBD27_000825"
     CDS             1643..2347
                     /locus_tag="VBD27_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO7024854.1"
                     /translation="MQKFKERFDTLSDAILAIAMTILVLEIKTPTTMGDIGDFTRNIG
                     LFIVSFVVVFNFWYERAQNSLDAQKTNDEIIALDIIEHLGICLIPLFTKFMISFENHN
                     FAVMAYGLLTLLVGLTSDIIRIRLASYDLVTIPSELKERVIKVMTTFAIRSVVVRFII
                     IILAYFLPEVGIFAYLVIPLFMFWRRSRAGKYYTKKGIEAPSYIKLFIQIGERVPEDF
                     TNSSSGISGVKRLYKK"
     gene            2514..3143
                     /gene="gmk"
                     /locus_tag="VBD27_000826"
     CDS             2514..3143
                     /gene="gmk"
                     /locus_tag="VBD27_000826"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO7024855.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFDYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEALIKEGQMLEYAEYVGNYYGTPLSYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFIFLTPPDLEELEERLVGRGTDSPEVIAQRIERAKEEIALM
                     REYDYAVVNDQVSLAAERVKRVIEAEHYRVDRVIGRYTNMVKETDKKLS"
     gene            3166..3480
                     /locus_tag="VBD27_000827"
     CDS             3166..3480
                     /locus_tag="VBD27_000827"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO7024856.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGEKATQDFKSV
                     KSTLRALEEIESGNVVIHPDPSAKRASVRARIEAERLAKEEEERKIKEQIAKEKEDGE
                     KI"
     gene            3554..5944
                     /locus_tag="VBD27_000828"
     CDS             3554..5944
                     /locus_tag="VBD27_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO7024857.1"
                     /translation="MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGARVHVPFG
                     RGNRLLQGFVVGFRDDDELETKDIAGVLDFEPVLNQEQLDLADQMRHTVFSYKISILK
                     SMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
                     EVQYLAKDKKTIKTEKIYKINRTLLEESQIAARAKKRLELKEFLLENPQPGRLTALNK
                     QFSSPVVNFFREEGIIEVIEKEASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDRIGK
                     DQNKPFLLEGITGSGKTEVYLHVIDNVLKLGKTAIVLVPEISLTPQMTNRFISRFGKR
                     VAIMHSGLSDGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQE
                     SNPRYHARDVALLRAEYHKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIP
                     QVEIIDFRNFIGQQEVSNFTPYLLDKIRDRLDKKEQVVLMLNRRGYSSFIMCRNCGYV
                     DQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKAYEELLK
                     VIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTSLNLPDFRSSERTFQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEA
                     FYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSYEVLSLLKQGFSDKVKLLGPT
                     PKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDITQDKENRDLRLAIDHEPQNMM"
     gene            5991..6926
                     /locus_tag="VBD27_000829"
     CDS             5991..6926
                     /locus_tag="VBD27_000829"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO7024858.1"
                     /translation="MTKLLFMGTPDFSATVLKGILADGKYDVLAVVTQPDRAVGRKKE
                     IKMTPVKEVALENNIPVYQPEKLSGSPELEQLMTLGADGIVTAAFGQFLPTKLLESVG
                     FAINVHASLLPKYRGGAPIHYAIINGEKEAGVTIMEMVAKMDAGDMVSKASVEITDED
                     NVGTMFDRLAVVGRDLLLDTLPGYLSGDIKPIPQNEEEVSFSPNISPDEERIDWNKSS
                     RDIFNHVRGMYPWPVAHTLLEGNRFKLYEVTMSEGKGSPGQVIAKTKNSLTVATGDGA
                     IELKSVQPAGKPRMDIKDFLNGVGRNLEIGDKFGE"
     gene            6916..8238
                     /gene="rsmB"
                     /locus_tag="VBD27_000830"
     CDS             6916..8238
                     /gene="rsmB"
                     /locus_tag="VBD27_000830"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO7024859.1"
                     /translation="MANDWKKSARGLALMTLEEVFDKGAYSNIALNKSLKKSRLSDKD
                     RALVTEIVYGTVARKITLEWYLSHFIVDRDKLELWVYHLLLLSLYQLLYLDNIPDHAI
                     VNDAVTIAKNRGNKKGAEKLINAVLRRVSSETLPEIASIKRQNKRYSVAYSMPVWLVK
                     KLIDQYGETRALAIMESLFERNKASLRVTDLSQKQTIKETLNVRDSHIAETALVADSG
                     NFASTSFFQDGLITIQDESSQLVAPTLKVSGNDQVLDACSAPGGKTSHIASYLTTGAV
                     TALDLYDHKLELVMENAKRLGLSDKIKTKKLDASKAHEYFPEDTFDKILVDAPCSGIG
                     LIRRKPDIKYNKANQDFEALQEIQLSILSSVCQTLRKGGIITYSTCTIFEEENFQVIE
                     KFLENHPNFEQVELSHTQEDIVKRGCISISPEQYHTDGFFIGQVKRIL"
     gene            8276..9013
                     /locus_tag="VBD27_000831"
     CDS             8276..9013
                     /locus_tag="VBD27_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEO7024860.1"
                     /translation="MEISLLTDIGQRRSNNQDFINQFENKAGVPLIILADGMGGHRAG
                     NIASEMTVTDLGSDWAETDFSELSEIRDWMLVSIETENRKIYELGQSDDYKGMGTTIE
                     AVAIVGDNIIFAHVGDSRIGIVRQGEYHLLTSDHSLVNELVKAGQLTEEEAASHPQKN
                     IITQSIGQANPVEPDLGVHLLEEGDYLVVNSDGLTNMLSNADIATVLTQEKTLDDKNQ
                     DLITLANHRGGLDNITVALVYVESEAV"
     gene            9013..10968
                     /gene="pknB"
                     /locus_tag="VBD27_000832"
     CDS             9013..10968
                     /gene="pknB"
                     /locus_tag="VBD27_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEO7024861.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVL
                     RTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIGEEDGQQFLVMEYVDGFDLKKYI
                     QDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSILAENKSVPQALENIVIKATAKKLTDRYKTTYEMGRDLSTALSST
                     RHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTEKITPDSASNDKTKS
                     KKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENS
                     GLKVGAIRNIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNY
                     KDILKDLQGKGVKKSLIKVKRKINNDYTTGTILAQSLPEGTSFNPDGNKKLTLTVAVN
                     DPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQAVVTPPSSSKIASQDPY
                     YGGEVGLRRGDKVKLYLLGSKTTNNSSSTPIDSSASSSTGTTTSDSVSSSTDASTSDS
                     SSTSTSSSTLPSDSTTNTGTANNPLTQ"
     gene            11127..11822
                     /locus_tag="VBD27_000833"
     CDS             11127..11822
                     /locus_tag="VBD27_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO7024862.1"
                     /translation="MKKFQFFLLVEAVVLVMGLMKILSDDWTSFIFILALILLALRFY
                     NNDSRHNFLLTTSLLLLFLIFMLNPYIIAAVVFAVLYVLINHFSQVKKKNRYALIQFK
                     NHQLDVKTTRNQWLGTDQHESDFYAFEDINIIRISGTDTIDLTNVIVSGQDNVIIIQK
                     VFGDTKVLVPLDVAVKADISSVYGSVQYFDFEEYDLRNESIKLSQEEEYYLLKRVKLV
                     VNTIAGKVEVSRK"
     gene            11819..12838
                     /locus_tag="VBD27_000834"
     CDS             11819..12838
                     /locus_tag="VBD27_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7024863.1"
                     /translation="MKKHHYFLAFFYGSVIIFAICFVIIDSLGVNLVHLYQTSRLWLI
                     EQLIFSIFFLSLAVTILLLLTWFLLDDNSKRQINHNLRRILNNQSINVTDDGTEISTN
                     IQRLSKKMNLMTASLQSKENSRILKSQEIVKQERKRIARDLHDTVSQDLFAASMVLSG
                     IAQNVSQLDVDQVGSQLLAVEEMLQHAQNDLRILLLHLRPVELENKTLSEGFKMILKE
                     LTDKSDIEVVYHESILTLPKKIEDNIFRIGQEFISNTLKHSQASRLEVYLNQTENELQ
                     LKMIDNGIGFDMDSVYDLSYGLKNIEDRVEDLAGNLQLLSQPGKGVAMDIRLPLVNQS
                     EDKNG"
     gene            12831..13472
                     /locus_tag="VBD27_000835"
     CDS             12831..13472
                     /locus_tag="VBD27_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7024864.1"
                     /translation="MDKIKIVLVDDHEMVRLGLKSFLNLQADVEVIGEASNGLEGIKK
                     ALELRPDVVVMDLVMPEMDGVEATLALLKDWPEAAILVLTSYLDNEKIYPVIEAGAKG
                     YMLKTSSAAEILNAIRKVARGEQAIENEVDKKIKAHDKCPALHEGLTARERDILNLLA
                     KGYDNQRIADELFISLKTVKTHVSNILGKLNVADRTQAVVYAFQHHLVPQDDE"
     gene            13514..14914
                     /locus_tag="VBD27_000836"
     CDS             13514..14914
                     /locus_tag="VBD27_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEO7024865.1"
                     /translation="MDAKTKYKAKKIKAVFFDIDDTLRVKDTGYMPPSILKVFKALKD
                     KGIVVGIASGRARYGVPKEVQDLNADYCVKLNGAYVKDKDKNIIFHRPIPAEYVEQYK
                     KWADTVGIKYGLAGRHEAVLSDRDDLVNDAIDIVYSDLEVNPDFNKEHDIYQMWTFED
                     KGDSLHLPEPLDEHLRLIRWHDHSSDVVLKGTSKALGVSKVVEHLGLKPENILVFGDE
                     LNDLELFDYAGLAVAMGVSHPEAQKKADFITKKVEEDGILYALEELGLIEKELTFPQV
                     DIENTEGPVAVIKTNHGDMTVKLFPDHAPKTVANFIGLAKQGYYDGIIFHRIIPDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNVRGALSMANAGPNTNGSQFFIVQNTKIP
                     YAKKELERGGWPTPIAELYAGQGGTPHLDRRHSVFGQLVNQSSFEVLDEIAAVETGSQ
                     DKPLEDVVILTIEVKE"
     gene            14916..15272
                     /locus_tag="VBD27_000837"
     CDS             14916..15272
                     /locus_tag="VBD27_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEO7024866.1"
                     /translation="MKIGDKLKGTVTGIRPYGAFVSLEDGRTGLIHISEIKTGYIDNI
                     YDVLSVGDEVYVQVIDVDEFTQKASLSLRTLEEERHHIQHRHRFSNNRLKIGFKPLEE
                     NLPSWVEEGLAYLNNK"
     gene            complement(15318..16094)
                     /locus_tag="VBD27_000838"
     CDS             complement(15318..16094)
                     /locus_tag="VBD27_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEO7024867.1"
                     /translation="MTNEKGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KMVPETMRDAITNESVIVGEEKSVDDIIEEVLKDIDFYEESGGGITLSGGEIFAQFEF
                     AKAILKRAKSLGIHTAIETTAYTRHEQFIDLIQYVDFIYTDLKHYNSLKHQEKTMVKN
                     ASIIKNIHYAFANGKTIVLRIPVIPNFNDSLEDAEEFACLFDRLDIRQVQLLPFHQFG
                     QNKYRLLNRQYEMEEIAALHPEDLLDYQAIFSKYNIHCYF"
BASE COUNT         5335 a         2498 c         3281 g         4981 t
ORIGIN      
        1 atcactaccc tttttggact ttatttttta cgctagcttt tgtaggagca tatttatact
       61 ctattattat caggggcgaa tcaaagtatc acatgattgc ctggtttaca atgttgacat
      121 atattttcta catgacagtt tttataaata gtggtttcat ctggtatatt tactttctga
      181 gtaatttact cgtctatcgc tttagagata aattgaagtc ttttagattc attagttttg
      241 cttgcactct agccacaatg gtatttttat gcttctttaa ggcatcagac tttggtgata
      301 gaatcatgtt tctaattgtt cctatttttt gtatcggtta catgtggatt gctattgaaa
      361 atagaaatag cgaagaacaa agagaaaaaa ttgcagagca aaatcagtat atcaatattt
      421 tgtcagctga aaatgaacgt aaccgtatag ggcgtgattt gcatgatagt ttaggacata
      481 catttgctat gatgacacta aagacagaac tagcgttaaa gttgttagaa aaaaggaact
      541 atgataaggt acaaaaagaa ctatcagaat taaatcatat tagtcatcaa tcgatgagtg
      601 aggtccgtca aattgtttct aacctcaagt atcgtacagt agtggaagaa attgatgagt
      661 tgtaccgctt gtttcaatta tctaacatca agttaacagt tgttaataag ttagagacgt
      721 cacaactaag tccagtaaca caatctacaa tcacaatgat attaaaggaa ttatccaata
      781 atattgttaa gcatgctgaa gctgatagcg tggagttgag tttagtaagg caaggtgcta
      841 ctataaatat tgagatgatt gataatggtt gtggttttac aaaccttgac ggagatgagt
      901 tgcatagtat tcaagagaga ttaactatcg ttgaagggac actaacgatt ttgtcccgaa
      961 gtaaaccaac tcatattcag gtgatattga aagaggtata agatgaaatt acttgttgct
     1021 gaggatcagt ccatgctacg agacgctatg tgccaattac ttttgatgga agaaagtgtt
     1081 tctactattg atcaagctgg aaatggtgaa gaagctattg ccattttatc caataaagcc
     1141 atagatgttg caatattgga tgttgagatg ccgatattat caggtttaga tgtcttagag
     1201 tgggttagaa aataccagaa tgttaaggtc ataattgtaa caacctttaa gcggtcaggt
     1261 tatttccaaa gggctattcg ttcaaatgta gatgcttatg ttttgaaaga ccgctcagta
     1321 gctgatttga tgaaaacgat tcaaaaagtt ttatctgggg gtaaagaata ttcgcctgaa
     1381 ttaatggaaa acgttatatc taatcctttg agtaagcaag aaataaaaat tctttcccta
     1441 attgctcaag ggaagaccag caaagaaatt gcatgcactt tatttttatc gaatggtaca
     1501 gttcgtaatt acacgtcaaa tatttttgat aaattaggag ttaataatcg tttggaggca
     1561 cttaaaatag cacgagaaaa ggattggctg tgatagtttt tttccagtcg agatgccata
     1621 atgtattaag agggatatat gtatgcaaaa atttaaagaa cgttttgata cgcttagtga
     1681 tgcaattttg gccattgcta tgacgatttt agttttggag ataaaaactc cgactacgat
     1741 gggtgatatt ggtgacttta cacggaacat aggtttattt attgttagct ttgttgttgt
     1801 ttttaatttt tggtacgaaa gggcacaaaa tagcctagat gctcaaaaga ctaatgatga
     1861 aattatagct cttgatatca ttgaacacct aggtatttgt ttgattcctc tctttactaa
     1921 atttatgatt tcttttgaga atcataattt tgctgttatg gcttatggac tcttaacgtt
     1981 attagttggt ctcacatcag acattattcg gattcgtttg gcaagctatg atttagtgac
     2041 cattcctagt gaattgaaag agcgggttat aaaagtcatg acaacttttg ccattcgttc
     2101 agttgtggtt cgctttatca ttattatcct cgcttatttt ctacctgagg tagggatatt
     2161 tgcatattta gtcataccgc tctttatgtt ttggcgtcgc tctagagcag gaaaatacta
     2221 tactaaaaag ggcattgagg caccctctta tatcaagctt tttatccaaa ttggagagag
     2281 agttcctgag gattttacta attcaagtag tggaataagt ggggtaaaaa gactctataa
     2341 aaaataatag gagaccttga aaaaaaggtt tctttttgtt aaactaatga gaagaaactt
     2401 tatttaggga gcaatttatg aattgctata aaagttaata gttaaggtaa gaaccagatt
     2461 gttctggtct ttttaacact taacacccga tctattaatt tgaggagtgt gtaatgtctg
     2521 aacgtggttt gctaattgtt ttctcaggac catctggtgt tggtaagggg acagttaggc
     2581 aagaaatttt ttcaacccct gaccataaat ttgattattc cgtttcaatg actactcgtc
     2641 cacaacgccc aggcgaagtt gatggagttg attatttctt ccgtacacgt gaagagtttg
     2701 aagcgttgat taaagaaggt caaatgcttg agtatgcgga gtatgttggt aactactatg
     2761 gaactccttt aagttatgtc aacgagactt tagacaaagg gattgatgtt tttcttgaaa
     2821 tagaagttca gggagcgctt caagttaaat cgaaggttcc ggacggtgtt ttcattttct
     2881 taacaccacc agatttagag gaattagaag agcgtttagt aggacgtggg acagatagtc
     2941 cagaagtgat tgcccaacgt attgaacgtg ccaaagaaga aattgctttg atgagggagt
     3001 atgactatgc tgttgtcaat gatcaagtgt cattagcagc agaacgtgtt aaacgtgtca
     3061 ttgaagcaga acattatcgt gtggatcgtg ttattgggcg ttataccaac atggtcaaag
     3121 aaactgataa aaaattaagt taaaaataga aaagagaatt gccttatgat gttaaaacct
     3181 tctatcgaca ctttgttgga taaagttcct tcaaaatatt ctttagttat tttacaagct
     3241 aagcgtgctc atgaattgga agctggtgag aaagccactc aagattttaa atctgtgaaa
     3301 tcaacacttc gcgctttaga agaaatcgag tcaggtaatg ttgtaattca cccagatcca
     3361 tcagcaaaac gtgcctcagt tcgcgctcgt atcgaagctg aacgccttgc taaagaagaa
     3421 gaagaacgta aaatcaaaga gcaaattgct aaagaaaaag aagacggcga aaaaatttaa
     3481 gcaagtcaaa agtgagaggt tgaaacattc tgtatcagcc tcttttcata ttttagagaa
     3541 ttagggaggg tttatggcaa gaaaattagc acaggttatt gttgatattc ctctgatgca
     3601 gacagataaa ccattttcgt atgccattcc taaagatttg gaagatttgg tacaagtagg
     3661 agcacgcgtt catgttcctt ttggaagagg gaatcgtctt ttgcaaggct ttgttgtcgg
     3721 tttcagagat gatgatgaat tagagactaa ggatattgcg ggggtcctag attttgaacc
     3781 tgttttaaat caggaacaac tagacttagc tgatcagatg cgccatactg tcttttctta
     3841 taaaatatct attttgaaat caatgttacc aagtcttttg aattcgcaat atgataaact
     3901 cttgttagca acagacacat taccatcaga agatagagaa gatctatttg gtcataaaac
     3961 ggaaattgtc ttttcaagtt tgtcttctca agatgccaaa aaagcaggca gattaattca
     4021 aaaaggtttc atagaagttc aatatttagc caaagataag aaaacaataa aaacagaaaa
     4081 gatttacaaa atcaacagaa ctctgttaga agagagtcaa atagcagcaa gagcgaaaaa
     4141 gagactggag ttgaaagaat tcctgttaga aaatcctcaa ccagggcgcc tcacagcttt
     4201 aaacaaacaa ttttcatctc ctgtagttaa ttttttcaga gaagaaggaa tcattgaggt
     4261 cattgaaaaa gaagctagta gatctgataa ttattttaaa ggaatcctaa aaacggattt
     4321 tttggattta aatcaggaac aagctaaagt agttaaaata gttgttgacc gaattggtaa
     4381 ggatcagaat aaacccttct tgttggaggg gattacaggg tctggtaaga cagaggtata
     4441 tcttcatgtc attgataacg ttttaaagtt gggaaaaaca gcgattgttc tcgttccaga
     4501 aatttcttta actccccaga tgacgaatcg ttttatttcc cgttttggaa agcgagttgc
     4561 tattatgcac tcgggcttgt cagatggtga aaaatttgat gagtggcgaa agataaaatc
     4621 aggtcaagct aaagttgtcg ttggagctcg ttcagcaatt tttgctcctt tggaaaatat
     4681 cggagccatc attatagatg aagaacatga atcaacttat aagcaagaat ctaacccaag
     4741 gtatcatgcg cgcgatgtgg ccttgctacg tgcagaatat cataaagctg tgttactaat
     4801 gggatcagca actccaagta tcgaaagccg agctagagct agtcgggatg tttataaatt
     4861 tttagaatta aaacatcgtg ctaatcctaa agcacgtatt cctcaggttg aaattattga
     4921 ttttagaaat tttattggtc aacaagaagt tagtaatttc acaccatatt tactagataa
     4981 aatccgagat agattagata agaaagagca agtggttctt atgctcaatc gtagaggtta
     5041 ttctagtttt attatgtgtc gaaactgcgg ctacgttgat caatgtccta attgtgacat
     5101 ttctctgact ttacatatgg ctacaaagac catgaattgt cattattgtg ggtttgaaaa
     5161 gcctattcca agaacatgtc caaattgcaa tagtaaaagt atctcttact atggtactgg
     5221 aacccaaaaa gcttatgaag aacttttgaa agttatccca gacgcaaaga ttttacggat
     5281 ggatgttgat acgacaaggc aaaaaggcgg tcatgagtct attttgaagc gtttcggaaa
     5341 tcatgaagct gatattttat tgggaacaca gatgattgct aagggattgg attttccaaa
     5401 tgttacttta gttggagtac taaatgcaga tacttctctg aatttacctg attttcgatc
     5461 atcagagcga acttttcagc tattaactca agtggctgga cgtgctgggc gtgctgaaaa
     5521 agaaggagag gttgtcattc aaacttataa tcctaatcat tatgctattc aattagccca
     5581 aaagcaagat tttgaagctt tttatcaata tgaaatgaat atccgtcgac aacttggtta
     5641 tcctccttat tattttacag ttggtttaac attatcgcat aaagatgaag agtggttaat
     5701 acgaaaatct tatgaagtat taagtttatt gaagcaaggc tttagtgata aagtaaagtt
     5761 gttaggtcca acgccaaaac caattgcaag aacacataac ctttatcact accaaattat
     5821 tatcaagtat cgatttgagg acaaccttga acttgttttg aatcgcttat tagacatcac
     5881 tcaagacaag gagaatagag atcttcgctt agcaatagac cacgaaccgc aaaatatgat
     5941 gtagaatagt gctatgatat aattgagaaa agaaagacga ggaaaaagca atgacaaaat
     6001 tattattcat gggtacccct gatttttcag cgacggtttt aaaaggtatt ttagcagatg
     6061 gtaagtatga tgttttagct gttgtgacgc aaccagatcg tgctgtaggt cgtaaaaaag
     6121 aaattaaaat gacccctgta aaagaagtgg ccttggaaaa caacattcct gtgtatcagc
     6181 cggaaaaact atctggaagt ccagaattag agcaactaat gaccctaggt gcagatggta
     6241 ttgtaacagc tgcttttggt caattcttac caacaaagct tttggaatcg gtaggttttg
     6301 caattaatgt acatgcttct ctattaccaa aatatcgtgg tggggcacca attcattatg
     6361 ccattattaa tggcgaaaaa gaagctgggg ttaccatcat ggagatggtt gcaaagatgg
     6421 atgcagggga tatggtatca aaagcttccg ttgagataac tgatgaagac aatgtaggga
     6481 caatgtttga tagactcgcc gttgttggtc gtgacctttt attagatacg cttccaggtt
     6541 atctatcagg tgatattaag ccgataccac aaaatgaaga agaggtaagt ttttctccta
     6601 atattagtcc agatgaagag agaattgatt ggaataaatc atcgcgcgac atttttaacc
     6661 atgttcgagg gatgtatcca tggccagttg cacataccct tttggaggga aatcgtttta
     6721 aactttatga agtaacaatg tcagaaggaa agggctcacc gggtcaagtg atcgctaaga
     6781 caaagaactc attgacagta gcaactggtg acggagctat tgaactcaaa tcagttcaac
     6841 cagcagggaa acctcgtatg gatataaaag actttttaaa tggtgttggt cgtaatttag
     6901 aaataggaga taaatttggc gaatgattgg aaaaagagtg cgcgtggttt agctttaatg
     6961 actttagaag aagtttttga taaaggagct tattctaata tcgcacttaa taagagttta
     7021 aaaaaatcta gactatcaga taaagatcgt gccttagtaa cagaaattgt ctatggaact
     7081 gtggctagaa aaataacgct agaatggtat ctatcacact ttatagttga tcgtgataag
     7141 cttgaattat gggtatatca tctcttgcta ctgagtcttt atcaactact ctatctggac
     7201 aacatcccag atcacgctat tgttaatgat gctgtcacta ttgctaagaa tcgaggcaat
     7261 aaaaagggtg ctgaaaagtt aattaatgcc gttttacgaa gagtaagttc ggagacgctc
     7321 cctgaaattg catcaataaa acgccaaaac aaacgttact ctgttgctta ttccatgcca
     7381 gtgtggctcg taaaaaaatt aattgaccaa tatggtgaga caagagcttt agcaattatg
     7441 gagagtttat ttgagcgaaa taaagccagt cttcgtgtga ctgacttatc gcaaaaacaa
     7501 actattaaag aaactttaaa tgttagagat tcacatattg cagagacagc tttagttgca
     7561 gactcaggaa attttgcatc gacatcattt ttccaagatg gcctcattac tatccaagat
     7621 gaatctagtc aattagttgc tccaacactc aaagtctcgg gtaatgacca agtattggat
     7681 gcatgtagtg caccaggagg aaaaacaagt catatcgcct catatttgac tacaggggca
     7741 gtaactgctt tagatttgta tgatcataaa ttggaattgg ttatggaaaa tgcaaaacgc
     7801 ctaggtctgt cagataagat aaaaactaaa aagttagatg caagtaaagc tcatgagtat
     7861 tttcctgagg atacttttga caaaatttta gtagacgcac cttgttcagg tatcgggcta
     7921 attcgccgta aaccagatat taagtacaat aaagctaatc aagactttga agccctccaa
     7981 gagattcaat tgtctatcct atctagcgtt tgtcaaacgc tacgtaaagg tggtataata
     8041 acctatagca cttgtaccat ttttgaggaa gaaaattttc aagttattga aaaattctta
     8101 gaaaaccacc ccaattttga gcaagtagaa ctgagtcata cacaagagga tattgtaaag
     8161 agaggctgta ttagtataag tcctgaacaa tatcatacag atggcttttt tattggacaa
     8221 gtcaaacgta tcttgtagtg tctaggagga aactgacatc gtcagaaaaa aatatatgga
     8281 aatatctctt ttaacagata ttgggcaacg tcgttccaat aatcaagatt ttattaatca
     8341 atttgaaaat aaagccggcg tcccactaat catcttagca gatggtatgg gaggacatcg
     8401 tgcaggaaat attgctagcg agatgactgt aactgatcta ggaagtgatt gggcggagac
     8461 agatttttct gagttatcag aaattcgtga ttggatgctt gtctctattg aaactgagaa
     8521 tcgcaaaata tatgaattag gtcagtcaga tgattacaaa ggtatgggaa ctaccattga
     8581 agcagttgcc attgtaggag ataatattat tttcgcacat gtaggagatt cacgtatcgg
     8641 tatcgttcgt caaggggagt atcatttatt gacgagtgat cattctcttg ttaatgaatt
     8701 ggtaaaagcc ggccaattaa cagaagaaga agctgctagt catcctcaaa aaaatattat
     8761 tacgcaatca attggtcaag ccaatccagt cgaacctgat ttaggagttc atctacttga
     8821 agaaggtgac taccttgtag ttaatagtga tggactaact aatatgttga gtaatgccga
     8881 tattgctaca gtactaacac aagaaaaaac cttagatgat aagaatcaag atttaatcac
     8941 tctagcaaac catagaggag gtcttgataa tatcactgtg gcactagttt acgttgaaag
     9001 tgaggcggtt taatgattca gattggcaaa ttatttgctg gtcgttatcg gatccttaaa
     9061 tcaatcggac gaggtggaat ggcggatgtt tatctagctc gagatctaat tttagataat
     9121 gaagaggtcg caataaaagt attgcgaacc aattatcaaa cagatcagat tgcggttgct
     9181 cgcttccaga gagaagcacg agcaatggct gagctgacac atcctaatat tgtcgctatt
     9241 cgtgatatag gtgaagaaga cggacagcag ttcttagtaa tggaatatgt tgatggattc
     9301 gatttaaaaa agtatattca agacaatgca cccctatcaa ataatgaagt tgttcgaatc
     9361 atgaatgaag tcctttcagc catgtcactg gcacatcaga aaggtatcgt acaccgtgat
     9421 ttaaaacctc aaaatatttt attaactaaa aaagggactg taaaagttac agatttcggt
     9481 attgcggtag cgtttgctga aactagttta acgcaaacga attctatgtt aggtagtgtt
     9541 cattatttat cgccagagca ggcgcgtggt tcaaaagcga ctgttcagag tgatatttat
     9601 gccatgggaa tcatgctttt tgaaatgtta actggccata ttccttatga tggggatagt
     9661 gcagttacta ttgccctaca gcacttccaa aaaccactac catcaatcct tgcagaaaat
     9721 aaaagcgttc cacaagcttt ggaaaacatt gtcattaaag caacagctaa aaagctaaca
     9781 gatcgctata agacaactta tgagatggga cgtgatttat caactgctct aagctcgaca
     9841 cgtcaccgtg aaccaaaact tgtttttaat gatactgaga gtactaagac actaccaaaa
     9901 gtaacatcta cagtatcatc tttaacgaca gaacaactgt tgcgtaatca aaaacaggcc
     9961 aaaacaacag aaaaaataac tcctgattct gcgagtaacg ataaaacgaa gtctaaaaag
    10021 aaggcttctc atcgacttct tggtacaatc atgaaattat tctttgcact ctgtgttgta
    10081 ggaataattg tatttgctta taaaatttta gtaagtccta cgactattcg tgttccagat
    10141 gttagcaaca aaacagtagc tcaagcaaaa atgacattgg aaaattctgg attgaaagta
    10201 ggagctattc gtaatattga gagtgatagt gtttcagaag gccttgtcgt aaaaacagac
    10261 cctgctgcag gacgttctcg tagagaaggg gctaaagtta atctttatat tgccactcca
    10321 aacaagagtt tcactctagg taattataag gaacataatt acaaagatat cctcaaagat
    10381 cttcaaggca aaggtgttaa aaaatcttta atcaaggtta agcgtaaaat taataatgat
    10441 tatactactg gtacaatctt agcacagtcg cttccagaag gaacatcttt taatcctgat
    10501 ggtaataaga aattaacact tacggttgct gttaatgatc caatgattat gcctgatgta
    10561 acaggtatga cagttggaga agtaatagaa accttgacag acctcggatt agatgcagat
    10621 aatcttgtct tctatcaaat gcaaaatggc gtttatcaag cagtagtaac acctccatct
    10681 tcaagtaaga tagcaagtca ggatccttat tacggaggag aagttggatt aaggagagga
    10741 gataaagtta agctgtatct tcttggttct aaaacaacaa ataactcatc atctacgcca
    10801 atagattctt cggcttcaag ctcaacagga acaactacct cggattctgt aagctcatca
    10861 acggatgcta gcacttctga tagtagtagc acttctacaa gtagttcaac actaccaagt
    10921 gatagtacaa ctaatacagg aacagctaac aatccattaa ctcaataaaa ttaaaaagaa
    10981 gatgttaaag tcttcttttt aatttgctat ggtagagacc atgcctagaa aggttctgcc
    11041 aatatgccaa atttttctct caataatggt gcaaagtata gggctttttg ataaaatacg
    11101 tcatatagag taatggaagg attattatga aaaaatttca gttttttcta cttgttgaag
    11161 ccgttgtctt agttatggga ttaatgaaaa ttttatcaga tgactggaca agttttattt
    11221 ttattctcgc tttgattctt ttagccttaa gattttataa caacgatagt cgtcataatt
    11281 ttctgttaac gacaagtttg ttattactat ttttaatctt tatgcttaac ccttacatta
    11341 ttgcagccgt tgtttttgca gttttgtatg ttctaatcaa tcatttctcg caagttaaga
    11401 aaaaaaatcg ttatgctttg attcaattta agaaccatca attagatgtt aagacaacac
    11461 gaaaccaatg gttaggtact gatcagcacg aaagtgactt ttatgctttt gaagatatta
    11521 atattataag aatctctggg actgatacaa ttgatttaac aaatgttatt gtatctgggc
    11581 aggataatgt tattataatt caaaaagttt ttggtgatac caaagtattg gttcctttag
    11641 acgttgccgt aaaggctgat attagttctg tttacggtag tgtacagtac tttgattttg
    11701 aggagtatga tctaagaaat gaatctataa aattgtcaca agaggaagaa tattaccttt
    11761 tgaaacgtgt caaattagtt gtaaacacta tcgctgggaa agttgaggtt agtcgtaaat
    11821 gaaaaagcac cactactttt tagctttctt ctatggaagt gttattatat ttgccatttg
    11881 ttttgtcatt attgatagtt tgggtgttaa tttggtgcac ctctatcaaa cgtctcgttt
    11941 gtggctgatt gaacaactga ttttctctat tttctttcta tctttagcgg taactatcct
    12001 tttattgcta acttggtttt tattagacga taatagtaaa cgtcaaatta accataattt
    12061 acgtcgcatt ttaaataacc aatcaataaa tgttaccgat gatggtactg aaatatcgac
    12121 caatattcaa cggctctcta aaaaaatgaa tttaatgaca gcaagtctac aatctaagga
    12181 aaatagtcgt attttaaaaa gccaagaaat tgtgaaacaa gaacgtaagc gtattgcacg
    12241 tgatttacac gacacagtta gtcaggattt gttcgcagct tccatggttt tgtcagggat
    12301 tgcacagaat gtttctcagc tagacgttga tcaggttggc tcgcaattat tagcagtaga
    12361 ggaaatgctt cagcatgcac aaaatgattt gagaattctg ctacttcatt tgaggccagt
    12421 tgaattagaa aataagaccc tatctgaagg attcaaaatg atattgaaag aattgaccga
    12481 taagagcgac attgaagttg tttatcacga atcaattcta accttaccca aaaagattga
    12541 agacaatatt tttagaattg gtcaagaatt tatcagtaat actttaaagc attcacaagc
    12601 cagtcgtctc gaagtgtacc tcaatcaaac agagaatgaa ttgcagttaa aaatgattga
    12661 taatggtatt ggatttgata tggattctgt ctatgattta agttatggat taaaaaatat
    12721 tgaagatcga gtagaagatt tagcaggaaa tttacagctt ctcagtcagc ctggtaaagg
    12781 agttgctatg gatataagat tacctttagt aaatcagagt gaggataaaa atggataaaa
    12841 taaaaattgt acttgttgat gaccatgaga tggttcgtct cggtttaaaa agttttttaa
    12901 acttacaagc tgatgttgaa gtaataggtg aagcctcaaa tggtttagaa ggcataaaaa
    12961 aagcactgga gttacgtcca gacgttgttg ttatggattt agtaatgcct gaaatggacg
    13021 gtgtagaagc aaccttagca ttgttgaagg attggccaga agctgctatt ttagtcttaa
    13081 catcttactt ggacaatgaa aaaatttacc ctgttattga agcaggtgcc aaaggctata
    13141 tgttaaagac ctcaagtgca gcagagattc ttaatgccat tcgtaaagtt gcaaggggag
    13201 aacaagctat tgaaaatgaa gtagataaga aaattaaggc tcatgataaa tgtccagctt
    13261 tacatgaggg cttaacagca agggaacgtg atatccttaa tttattagcc aaaggttatg
    13321 acaatcaaag gattgctgat gaattattta tctcgttaaa gactgttaaa actcatgttt
    13381 ctaatatttt gggaaaacta aatgtggcag atcgaactca agcggttgtc tatgctttcc
    13441 aacaccattt ggtaccacaa gatgatgaat aagtgatata atagagacac tatgttaatg
    13501 aaagtggtta gtcatggacg caaaaactaa atataaagca aaaaaaataa aggcggtgtt
    13561 ttttgatatt gatgacacgc ttcgtgttaa agatacaggt tatatgccac catcgatctt
    13621 aaaagttttt aaagctttga aagataaagg aattgttgta ggtattgctt caggaagggc
    13681 acgatatggc gttccaaaag aagttcaaga tttaaatgcc gattattgtg taaaattaaa
    13741 tggagcttat gttaaggaca aggataagaa tatcatcttc catcgtccta ttcctgctga
    13801 atatgtagag caatataaga aatgggcaga cactgttggt attaagtacg gtttagctgg
    13861 tcgtcatgag gcagttttat cagatcgtga tgatttggta aatgatgcga ttgatattgt
    13921 gtattctgat ctcgaagtta atccagattt caataaagaa catgatattt atcagatgtg
    13981 gacgtttgaa gataaaggag atagccttca tctacctgaa ccgttagatg aacatcttcg
    14041 cttgatacgt tggcatgatc attcttcaga tgttgtgtta aaaggaacat cgaaggcttt
    14101 aggtgtttct aaagttgttg aacatttggg cttaaagcca gagaatatct tggttttcgg
    14161 agatgagctc aatgatttag aactctttga ttatgctgga ctagctgtgg cgatgggagt
    14221 gtcacaccca gaagctcaaa aaaaagcaga ttttatcaca aaaaaagtag aagaagatgg
    14281 cattttgtat gccctggagg aactaggttt gatagaaaaa gaattaacat ttccacaagt
    14341 agatattgaa aatacagaag gtcctgtggc tgtcattaaa acaaatcacg gggatatgac
    14401 tgtaaaactc ttcccggatc atgctcctaa aacggtagca aactttattg gtttagctaa
    14461 acaaggttat tatgatggta ttatcttcca tcgtattatt ccagatttta tgattcaggg
    14521 tggtgatcca actggtacag gtatgggtgg tgaatctatc tatggggaaa gttttgaaga
    14581 cgaattttcc gaagaattat ataatgttcg cggagctctt tcaatggcta atgctggacc
    14641 aaatacgaat ggcagccaat ttttcatcgt ccaaaataca aaaattcctt atgcgaaaaa
    14701 ggaattagag cgaggtggtt ggccaacacc aattgctgag ttgtatgctg gacaaggtgg
    14761 tacaccgcat ctagatcgtc gccacagtgt atttggacaa ttagtaaacc aaagttcttt
    14821 tgaggtactt gatgagattg cggcagtaga gactggttct caagacaaac cattggaaga
    14881 tgttgttatt ttaacgattg aggttaaaga ataaaatgaa gattggcgat aagttaaagg
    14941 gaacagtcac tggcattagg ccttatggag cctttgtttc tcttgaggat ggtaggactg
    15001 gacttatcca tatttctgaa attaaaacag gttacattga taatatttat gatgttctta
    15061 gtgtaggtga tgaagtctat gttcaggtta tagatgtaga tgaatttaca caaaaagcaa
    15121 gtttatcgct tcggaccctt gaagaagaaa gacatcatat tcagcatcgt catcgatttt
    15181 ctaacaatcg tcttaagata ggtttcaaac cacttgagga aaatttacct agttgggttg
    15241 aggaaggtct agcctacttg aataataaat aaacaaaaac ctagatttga ttaatgacaa
    15301 gtctaggttt taaatattta aaagtagcag tgaatattat acttgctaaa aatagcttga
    15361 taatcgagta gatcctctgg gtgtagtgca gcgatctctt ccatttcata ttgacgatta
    15421 agtaatcgat atttgttttg tccaaattgg tggaaaggta agagttgcac ttgacggata
    15481 tctaggcgat caaataaaca agcaaattct tcagcatctt ctaatgaatc gttaaaatta
    15541 ggtatgacag gaatacgtag cacaatcgtt ttaccgttgg caaaagcata atgaatgttt
    15601 ttgatgattg aagcattctt aaccatcgtc ttttcttggt gtttgaggct attgtaatgt
    15661 tttaaatcgg tgtaaataaa atcaacgtat tggataaggt ctatgaattg ttcatgtcta
    15721 gtataagcag ttgtttcaat agccgtgtgg attcccagtg atttagcacg ttttaaaatt
    15781 gccttggcaa attcaaattg tgcaaatatt tcaccgccag atagcgttat gcccccacca
    15841 gattcttcgt aaaaatcaat atcttttaaa acctcttcaa taatatcatc aaccgatttt
    15901 tcttcgccca cgatgacgct ttcattagta atggcatctc tcattgtttc tggaaccatt
    15961 ttttgcgact cgggattagc acaccaagga catctaagag gacaaccctt taagaaaacg
    16021 gttgttcgaa tacctggacc atcgtgaata ctaaaatgtt gaatgttgaa cacaatacct
    16081 ttttcatttg tcata
//
LOCUS       DATRQA010000036        15720 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.036, whole genome shotgun sequence.
ACCESSION   DATRQA010000036 DATRQA010000000
VERSION     DATRQA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15720
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.036"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..315
                     /locus_tag="VBD27_000839"
     CDS             <1..315
                     /locus_tag="VBD27_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024868.1"
                     /translation="KDSLPQSAPAVEDTGWQYIGNGFNYRKIGSMVTIKYDFATNGIN
                     QFTVGSMPTNLIPNEMMFAVTAWTVQLNVLNVQVSADGRILWFNPSKWAVNVKGQINW
                     II"
     gene            327..719
                     /locus_tag="VBD27_000840"
     CDS             327..719
                     /locus_tag="VBD27_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO7024869.1"
                     /translation="MLEFLNRYPVLLEDKSVKETKAILAFTSSTIKANFEVTLPAEEN
                     DKKFAETLKTCEKLIFEQLYKDKAEAEQFEKINDAIAKSKAQSDKAENMIKLMSATVN
                     DLIKTMADGGKLNDTTLNNASENSSTHI"
     gene            879..1181
                     /locus_tag="VBD27_000841"
     CDS             879..1181
                     /locus_tag="VBD27_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024870.1"
                     /translation="MNIDILQIGAASGAILSVVGLWAFVVNPFKTAMQKNEDTMSALK
                     DTIKELAYELKDSQRDREKIHKILDIHEQRLGKTEDDIIVNKEQIKTLFNRRNKYD"
     gene            1174..1401
                     /locus_tag="VBD27_000842"
     CDS             1174..1401
                     /locus_tag="VBD27_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO7024871.1"
                     /translation="MINLKLRLQNKVTLMAILGAIFLLAQQVGIKLPSNIADIANTAV
                     TLLVLLGVVTDPTTKGLSDSEQALNYHEPKK"
     gene            1527..2861
                     /locus_tag="VBD27_000843"
     CDS             1527..2861
                     /locus_tag="VBD27_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEO7024872.1"
                     /translation="MATYQEYKNRSNGNAYDIDGSFSAQCWDGYADYCRFLGVPYANC
                     TNTGYARDIWEQRHENGILNYFDEVETMQAGDVAIFMVVDGVTPYSHVAIFDSDAGGG
                     YGWFFGQNQGGTNGAYNLVKIPYSATYPTAFRPKVFKNAVTVTDNTGLNKGDYFIDVS
                     AYQQADLTATCQQAGTTKTIIKVSESIAWLSDRHQQQANTSDPIGYYHFGRFGGDSAL
                     AQREADLFLSNLPGKKVSYLVIDYEDSASADKQANTNAVIAFMDKIASAGYKPVYYSY
                     KPFTLNNVDYQQIIAKYPNSIWVAGYPDYEVRSEPLWDYFPSMDGVRWWQFTSVGIAG
                     GLDKNVVLLADDNSQVTVPKADKPQEAVSFNQRLDVNTKLDNSNTPYYEATLSTDYYV
                     ESKPNASSSDKEFIKAGTRVRVYEKVNGWSRINASQSDQWVEDKYLANATQV"
     gene            3025..3231
                     /locus_tag="VBD27_000844"
     CDS             3025..3231
                     /locus_tag="VBD27_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7024873.1"
                     /translation="MSLVEEKLKAKVEQASGSLKEGAGKLTGDKELEAKGFVEKTIAK
                     GKELADDAKDAVEEAVDAVKEKLK"
     gene            3343..3525
                     /locus_tag="VBD27_000845"
     CDS             3343..3525
                     /locus_tag="VBD27_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO7024874.1"
                     /translation="MLYIDEFKEAIEKGYISSDTVMVVRKNGKIFDYVLPGEPVRLWE
                     VATEEKVEEVLMELDK"
     gene            3738..4430
                     /locus_tag="VBD27_000846"
     CDS             3738..4430
                     /locus_tag="VBD27_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO7024875.1"
                     /translation="MSKVRLYIARHGKTMFNTIGRAQGWSDTPLTTFGELGIKELGWG
                     LKASNISFKEAFSSDSGRTLQTMEIILREVQQENIPYTRDKRIREWCFGSLDGGYDGD
                     LFNGVLPRVSNGDMSHLTHEEIANLICQVDTAGWAEPWAILSNRILSGFTAIAKKIED
                     IGGGNAIVVSHGMTIATFLWLIDHSTPRSLGLDNGSVSVVDFEDGTFSIQSIGDMSYR
                     EKGREILEKTLQ"
     gene            4427..5179
                     /locus_tag="VBD27_000847"
     CDS             4427..5179
                     /locus_tag="VBD27_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEO7024876.1"
                     /translation="MKKNKIIRFSLVGVLLAILCFSLFALLKPNSQQSSSQKLRNEDI
                     KKTSSQKRNKKLRLPAVSSKDWNLILVNRDHKHEELSPDVVPVENIYLDKRITKQATQ
                     FLEAARAIDSREHLISGYRSVAYQEKLFNSYVTQEMTSNPNLTRGQAEKLVKTYSQPA
                     GASEHQTGLAMDMSTVDSLNESDPRVVSQLKKIAPQYGFVLRFPDGKTAETGVGYEDW
                     HYRYVGVESAKYMVKHHLTLEEYITLLKENNQ"
     gene            5176..5751
                     /locus_tag="VBD27_000848"
     CDS             5176..5751
                     /locus_tag="VBD27_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO7024877.1"
                     /translation="MRKRFSLLNFIVVTFIFFFFILFPLLNHKGKVDANSRQSVTYTK
                     EEFIQKIVPDAQDLGKSYGIRPSFIIAQAALDSDFGEKILANKYHNLFGLLAEPGTPS
                     ITLNDSSTGKKQEKQFTHYKSWKYSMYDYLAHIKSGATGKKDSYTIMVSVKNPKTLVQ
                     KLQDSGFDNDKKYAKKMTEIIDLYDLTRYDK"
     gene            5846..6928
                     /gene="hrcA"
                     /locus_tag="VBD27_000849"
     CDS             5846..6928
                     /gene="hrcA"
                     /locus_tag="VBD27_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEO7024878.1"
                     /translation="MCKSANFRKTERGDVFVITQRQNDILNLIVELFTQTHEPVGSKA
                     LQRTIDSSSATIRNDMAKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQD
                     IYHVIKAFDFEAFKLEDMLQKASHILSEMTGYTSVILDVEPARQRLTGFDVVQLSNHD
                     ALAVMTLDESKPVTVQFAIPRNFLTRDLIAFKAIVEERLLDGSVMDIHYKLRTEIPQI
                     VQKYFVTTDNVLQLFDYVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLR
                     QGLKEGEMASVQVADSQEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLV
                     NIIGKVLAAKLGDYYRYLNSNHYEVH"
     gene            6931..7503
                     /gene="grpE"
                     /locus_tag="VBD27_000850"
     CDS             6931..7503
                     /gene="grpE"
                     /locus_tag="VBD27_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO7024879.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEEIVEEVIEEIP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            7684..9513
                     /gene="dnaK"
                     /locus_tag="VBD27_000851"
     CDS             7684..9513
                     /gene="dnaK"
                     /locus_tag="VBD27_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO7024880.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIQSNSGLTDEEIDKMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKKAQESGNLDDMKAKLEALNEKAQ
                     ALAVKLYEQAAAAQQAAQGAEGAQSADSSSKGDDVVDGEFTEK"
     gene            9802..10917
                     /gene="dnaJ"
                     /locus_tag="VBD27_000852"
     CDS             9802..10917
                     /gene="dnaJ"
                     /locus_tag="VBD27_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO7024881.1"
                     /translation="MNNTEFYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKEAGAEE
                     KYKEVQEAYETLSDTQKRAAYDQYGAAGANGGFGGFDGGGFGGFEDIFSSFFGGGGMR
                     NPNAPRQGDDLQYRVNLSFEEAIFGAEKEVSYNRESSCHTCSGSGAKPGTSPVTCQKC
                     HGSGVINVDTQTPLGTMRRQVTCDVCQGSGQEIKEKCPTCHGTGHEKKTHKVSVKIPA
                     GVETGQQIRLTGQGEAGFNGGPYGDLFVIINVLPSQQFERNGSTIYYTLNIGFVQAAL
                     GDTIDIPTVHGAVEMSIPAGTQTGKTFRLRGKGAPKLRGGGQGDQHVTVNIVTPTKLN
                     DAQKEALHAFAEASGDKMVHPKKKGFFDKVKDALDVD"
     gene            11035..12282
                     /locus_tag="VBD27_000853"
     CDS             11035..12282
                     /locus_tag="VBD27_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO7024882.1"
                     /translation="MVTKVEEIRSYLIASIQNGKLAPGDRLPSIRQLANQFSCNKDTV
                     QRVLMELRFDNYIYAKPRSGYYVFDSHQEEVEEGVSLPNSEIANIAYDDFRLCLNETL
                     IGREDYLFNYYYRQEGLLDLSKAVAKLMEETGVYVPLDDIVITAGTQQALFILTQVTF
                     PNRKSRVLIEEPTYPRMIELIKTQNLPYETIPRGTHGIDFQRLEEIFQTQSIKFFYVI
                     PRMHNPLGTSYNPVEMKRLIEMAEKYDVYIVEDDYMSDFASQSPLHYYDTHGRVIYLK
                     SFSKAIFPALRLAAICLPQALKSTFMAYKKLMDYDTNLILQKALALYIENGLYAKNSQ
                     YLKYRYQKDLANSKSILADHPNLPPYSLHHDSVLFDCSKLDNFKILRQYGDTLENYFC
                     QKSHQSLLQVKNDSCLKQFLGSL"
     gene            12353..13129
                     /gene="truA"
                     /locus_tag="VBD27_000854"
     CDS             12353..13129
                     /gene="truA"
                     /locus_tag="VBD27_000854"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO7024883.1"
                     /translation="MTRYKAQISYDGSAFSGFQRQPNCRTVQEEIERTLKRLNSGNDV
                     IIYGAGRTDAGVHAYGQVIHFDLPQARDVEKLRFGLDTQCPDDIDIVKVEQVSDDFHC
                     RYDKHIKTYEFLVDIGRPKNPMMRNYATHYPYPVIIELMQEAIKDLVGTHDFTGFTAS
                     GTSVENKVRTIFDAKIQFEASKNLLIFTFTGNGFLYKQVRNMVGTLLKIGNGRMPISQ
                     IKTILQAKNRDLAGPTAAGNGLYLKEIIYEDEECFSNFRK"
     gene            13092..13850
                     /locus_tag="VBD27_000855"
     CDS             13092..13850
                     /locus_tag="VBD27_000855"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO7024884.1"
                     /translation="MKTRNVLAISGNDIFSGGGLHADLATYVVNKLHGFVAVTCLTAM
                     SDKGFEVIPIEASILKQQLESLKDVEFGSIKLGLLPNVETAQVVLEFVKSKQECPVVL
                     DPVLVCKENHDLEVSQLREQLIAFFPYADVITPNLVETQLLTGLSIENLDQMKIAAEK
                     LYDMGAKHVVIKGGNRLNAEEATDLYYDGERFETYVFPVVDANNTGAGCTFASSIASQ
                     LAMGKNVEDAVKMSKGFVYQAIKASDKYGVVQHF"
     gene            13860..14324
                     /locus_tag="VBD27_000856"
     CDS             13860..14324
                     /locus_tag="VBD27_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7024885.1"
                     /translation="MRKEKTSQLVQLAIVTAISIVLGMFISIPTPTGFLTLLDAGIFF
                     AAFYFGKKEGAVVGALAGFLIDLLKGYPNWMFFSLLIHGAQGYLAGLPGRRRLLGLIS
                     ATLVMVLGYAIASGLMYGWGAVLPDIPGNIMQNMVGMVVGFALNKSLERVKK"
     gene            14321..14890
                     /locus_tag="VBD27_000857"
     CDS             14321..14890
                     /locus_tag="VBD27_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEO7024886.1"
                     /translation="MILDYNKLKQETKAIVVDIIERSALKKGQIFVLGLSSSEVSGGL
                     IGKNSSSEIGEIIVEVILKELHSRGIYLAVQGCEHVNRALVVEAELAERQQLEVVNVV
                     PNLHAGGSGQVAAFKLMTSPVEVEEIVAHAGIDIGDTSIGMHIKRVQVPLIPISRELG
                     GAHVTALASRPKLIGGARAGYTSDPIRKF"
     gene            complement(14927..>15720)
                     /locus_tag="VBD27_000858"
     CDS             complement(14927..>15720)
                     /locus_tag="VBD27_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEO7024887.1"
                     /translation="FTFFTKLISILLLIIAFVIVRQVINYLFEKTVNRSLAFSRQKVA
                     RQKTLAKLSHNVLNYTLYFFLFYWILSILGVPISSLLAGAGIAGVAIGLGAQGFLSDV
                     VNGFFILLENQFDVGDIINVGAVSGTVTNVGIRTTQIHDFDGTLHFIPNRNITIVSNK
                     SRSNMRAQIDIPLFVHTNLDQISDIVTKINEEYVSKHPAIVGEPTVFGPTTNANGQFV
                     YRINIFTQNGAQFDIYAEFYKLYQKAILEEGIDLPTYNFSNNHSR"
BASE COUNT         5236 a         2448 c         3366 g         4670 t
ORIGIN      
        1 aaagatagct taccgcaatc cgcaccagcg gtagaagata ctggttggca atacatcggc
       61 aacggtttta attacaggaa aattggtagc atggtcacta ttaaatatga ctttgcaaca
      121 aatggaataa accagtttac ggtcggttcc atgccaacga atttaattcc aaacgaaatg
      181 atgtttgcgg ttactgcgtg gactgtgcaa ttaaatgtat taaatgtaca agttagtgca
      241 gatggtcgta ttttatggtt caacccatca aaatgggcgg ttaatgttaa aggacaaatt
      301 aattggataa tttaaaagga ggaattatgc ttgaattttt gaatagatac ccagttttac
      361 tggaagataa aagtgtaaaa gagactaaag cgattttagc atttacgtct agcacgatta
      421 aagcaaattt tgaagtgacg ctaccagcag aagaaaatga taaaaaattt gctgaaactt
      481 taaaaacgtg tgaaaagctt atctttgagc aactttacaa agacaaagca gaagcagaac
      541 aatttgaaaa aattaatgac gcaattgcta agtcaaaggc gcaatcagat aaagcggaaa
      601 atatgattaa actgatgtca gcaactgtta acgatttgat taagacaatg gctgacggag
      661 ggaaattgaa tgatacaacg cttaacaacg ctagcgaaaa tagcagtaca catatttaaa
      721 aacaaaaaag gagaaaaaac aatgatgatt aattactttg caatgcagat tgaactaggg
      781 tggattacta ttgatgacgt tccagcattt tgtcgtgagc gagtacgtaa actaattgaa
      841 gtttctacgg ttggtacaga aggaaaatga ggcaatgaat gaacattgac atactacaaa
      901 ttggcgcagc aagcggggcg attttatcgg tagttggatt gtgggcgttt gttgttaatc
      961 cgtttaaaac agcgatgcaa aaaaacgaag atacaatgag cgcccttaaa gacacaataa
     1021 aagaactggc ttacgaacta aaagactcac agcgtgacag ggaaaagata cataaaatct
     1081 tggatatcca cgagcaacga ctcggaaaaa cagaagacga catcattgtc aacaaggaac
     1141 aaataaaaac attatttaat aggagaaata aatatgatta atttaaaatt acgactacaa
     1201 aacaaagtaa ctttgatggc tattttagga gctatatttt tgctagcaca gcaagtaggt
     1261 attaaactac catcaaatat tgcggatatt gccaacacag cagtaacgct tttggttttg
     1321 cttggagttg tgacagaccc aacaacaaaa gggctatcag atagcgaaca agcattgaat
     1381 taccacgagc caaaaaaata ggaggggaca tgcgagcaat cactaaaata gcaatggtac
     1441 tagcgatagc aatactgtac ataccgcttg cagtgattgc tttttttatt tatccgattt
     1501 atttgatttt tgaaaaggag gagtaaatgg caacgtatca agaatataaa aacaggtcaa
     1561 atggtaacgc ttatgacatt gatgggtcgt ttagtgcaca atgttgggac ggttatgctg
     1621 attactgtag atttttaggc gtaccgtacg caaactgtac aaacacagga tacgcaagag
     1681 acatttggga acaacggcac gaaaatggca ttttaaacta ctttgacgag gtagagacta
     1741 tgcaagctgg ggatgttgct atttttatgg tcgttgacgg tgtgacacct tatagccatg
     1801 tcgctatttt tgatagtgat gctggtggtg gctacggatg gttttttggt caaaatcaag
     1861 gtggaacaaa tggagcttat aatctagtaa aaataccata ttcagcaact tatcctactg
     1921 cgtttagacc aaaagttttt aaaaacgctg ttactgtaac tgataatacc ggtttaaata
     1981 aaggtgatta ctttatcgat gtatcggctt atcagcaagc ggatttaact gctacttgtc
     2041 agcaggcagg cactacaaaa acgattatca aggtatcaga gtctattgcg tggctatctg
     2101 acagacacca acaacaagct aacacgagtg acccgattgg ctattatcat tttgggcgtt
     2161 ttggcggtga tagtgcttta gcgcaaaggg aagcagactt gtttttgtct aacttaccag
     2221 gcaaaaaagt atcatactta gtcattgact acgaagattc cgcaagcgca gacaagcaag
     2281 ctaacacaaa cgcagttatt gcatttatgg ataaaatcgc tagcgctggt tacaagcctg
     2341 tttattacag ctataagcca tttacactta ataatgttga ttatcagcag attattgcta
     2401 aataccccaa cagtatttgg gtcgcaggct atcccgatta tgaggtgcgc tctgaaccgc
     2461 tgtgggacta ttttccgtca atggacggtg tgcggtggtg gcaatttaca agtgtcggaa
     2521 ttgctggggg gctagataaa aatgtcgttt tattagctga cgacaatagc caagtgactg
     2581 taccaaaagc agataaaccg caagaagcag ttagctttaa tcagcgttta gatgttaata
     2641 ctaagctaga taactcaaat acgccatact acgaagcaac ccttagcaca gactattatg
     2701 tagagtctaa gccaaacgca agtagttctg ataaagaatt tatcaaagca ggaactcgcg
     2761 taagagtcta cgaaaaagta aatggatggt cacgtattaa cgcttctcag tctgaccaat
     2821 gggtcgaaga taagtattta gctaatgcca cacaagtata aaacaggagg taaaattcct
     2881 ttagataaga caaatgccct cgcatacgcg cagacatgtt ctgatggggg gtttttttgc
     2941 taaaaaattg agggtgtttc atggcggaaa attttcaaca caaaagttac agagaacaag
     3001 ttgaactttt ggaatctaga ggtattgtct ttagtggaag aaaagctaaa agcaaaagtt
     3061 gaacaagcgt caggcagtct taaagaaggt gcagggaagc taaccggtga taaagagtta
     3121 gaagcaaaag gatttgtcga aaaaacaatt gctaaaggta aagaactagc agatgatgct
     3181 aaagatgctg ttgaagaggc agtagatgct gtcaaagaaa aactgaaata aatattaacc
     3241 gctcattaaa tgagcggtta tttttttgtc tatcagaaca gaaaaattta aaattgtcta
     3301 tttttaggat tttttatcga atagataagt ggaggataaa atatgttata tatagatgag
     3361 tttaaagaag cgattgaaaa aggatatatc agcagtgata cagtgatggt tgtgcgtaag
     3421 aacggaaaga tatttgatta tgtgttacct ggtgagcctg taagattgtg ggaagttgcg
     3481 acagaggaaa aagtggaaga agtgttgatg gaattagata aataaaaaaa gacttttcca
     3541 agtccaaaaa tcagatgctc cccagcgcaa aaattactac gttatttact acgttgtttt
     3601 taaaatcagt gtaattgacc agataatgta aggttgtaaa atgttgattt atcaagtgat
     3661 tgggcaatga tagcaaatga taccatatta aggttctttt ttgttataat ataataaaat
     3721 gcttgtgagg tgtcagtatg agtaaagttc gtttatatat cgctcgtcat ggtaaaacaa
     3781 tgtttaatac aattggtcgt gctcaggggt ggagtgatac tcctttaact acttttggag
     3841 aactcgggat taaagagctt ggttggggac taaaggcttc aaatattagt tttaaagagg
     3901 ctttttctag tgatagtgga cgtactttac aaactatgga aattattctc agggaagtgc
     3961 aacaagaaaa tattccctat acgagagata aacgaatccg cgagtggtgc tttggtagtt
     4021 tggatggagg atatgatggc gaccttttta atggcgttct gcctcgagtt tcaaacggag
     4081 atatgagcca tttgacacac gaagaaattg ctaatttgat ttgtcaagtt gatacggctg
     4141 gctgggcaga accgtgggca atattgagta accgtattct gtcaggcttc actgctatag
     4201 ctaaaaaaat agaggatatt gggggaggta atgcgattgt tgtcagtcat gggatgacta
     4261 ttgcgacttt tttatggtta atcgaccata gtacccctcg tagtttaggc ttagataatg
     4321 gtagtgtctc agtcgttgat tttgaagatg gaacattttc tattcaatct attggggata
     4381 tgtcctatcg agaaaaagga agagaaattt tagaaaaaac attgcaatga aaaaaaataa
     4441 aattatccga ttcagtttag ttggtgttct acttgcgata ctatgcttta gtctttttgc
     4501 tttattgaag cctaacagtc aacaatcatc atctcaaaag ttgaggaatg aggatataaa
     4561 aaagacatcc tctcaaaaaa gaaataagaa attacgatta ccagctgtat catcaaaaga
     4621 ttggaacttg attttggtca atcgtgacca taaacatgaa gaattaagtc cagatgtggt
     4681 gcctgttgaa aatatttatt tggataaacg tattacgaag caagctactc agtttttaga
     4741 ggctgctaga gcaattgatt cacgagaaca tttaatttcg ggttatcgta gtgttgccta
     4801 tcaggagaag ttgttcaatt cttatgttac tcaagagatg actagtaacc ctaatttgac
     4861 gaggggacaa gcagaaaagt tggtaaaaac ttactctcag cctgcaggtg ctagtgaaca
     4921 ccagactgga ttagcgatgg atatgagtac tgtagattct ttgaatgaga gcgatcctag
     4981 agtagtcagt cagttgaaaa agatagctcc acaatatggt tttgtcttac ggtttccgga
     5041 tggtaaaaca gcagaaacag gggtaggtta tgaagattgg cattaccgct atgttggggt
     5101 agagtctgca aaatatatgg tcaaacatca tttaacatta gaagaataca taactttatt
     5161 aaaggagaat aaccaatgag gaaacgtttt tccttgctaa attttattgt tgttactttt
     5221 attttctttt tctttattct ttttccgctt ttaaaccata agggaaaagt agatgctaat
     5281 tctaggcaga gtgttaccta caccaaagaa gaatttatac aaaaaattgt gccagatgcg
     5341 caagatctag gaaagtcgta cggtattcgt ccttcattta ttattgcaca ggcggctttg
     5401 gattctgatt tcggagagaa aatattagct aataagtatc ataatctgtt tggtttgctt
     5461 gcagaaccag gaacgccctc aattacctta aatgatagta gtacaggaaa aaaacaggaa
     5521 aagcaattta ctcattataa atcttggaag tattcaatgt atgattacct tgctcatata
     5581 aaatctggag cgacaggcaa aaaagattca tatactataa tggtgtctgt taaaaatcca
     5641 aaaactttag tgcaaaaatt acaagatagt ggttttgata atgacaaaaa gtacgctaaa
     5701 aaaatgacgg aaatcattga tttgtatgat ttaacaagat atgataagtg atattagcac
     5761 tctttgaata agagtgctaa tttttgatgt tttttccttg actatctttt aaaaaggagt
     5821 ataataaaat catagttagc agtcgatgtg taagagtgct aatttcagaa agaccgagag
     5881 gggtgatgtt tttgtgatta cacaacgtca aaatgatatt ttgaatttaa tcgttgagtt
     5941 atttacacaa actcatgaac cagtagggtc taaagcttta cagagaacga ttgattctag
     6001 tagcgccact attcgtaatg atatggcaaa actagagaag ttggggttat tggaaaaagc
     6061 acacacttct agtggacgga tgccgagtcc agctggtttt aagtattttg tagagcactc
     6121 ccttagacta gatagtattg atgagcaaga tatttatcat gttataaaag cttttgactt
     6181 tgaagcattc aaattagaag acatgcttca aaaagctagt catattttat cggagatgac
     6241 gggctataca tccgttattt tagatgtgga gccagctcgt caacgtttga cagggtttga
     6301 tgttgttcag cttagcaacc atgatgctct agcagtcatg actttagatg aatctaagcc
     6361 agtgacagtg cagtttgcta ttccaagaaa ctttttgaca cgtgacttga tagcatttaa
     6421 agctatcgta gaggagcgct tgctggatgg tagtgtcatg gatattcatt ataagttgag
     6481 gacagaaatt cctcagatcg ttcagaagta ttttgtaact acggataatg ttttacaact
     6541 atttgattat gttttttcag agctgttttt ggaaacggta tttgtagctg gcaaggtaaa
     6601 ttctttaaca tattcagatc tatcaacgta tcagtttttg gataatgagc agcaagtagc
     6661 aatttctttg aggcaaggtc tgaaagaggg tgagatggct tctgtccaag tggcagatag
     6721 tcaagaagcc gcacttgcag atgtgtcagt tttaactcac aaatttttga ttccttaccg
     6781 tggatttgga ctcctgagtt tgattggtcc tattgatatg gattatcgta ggtctgttag
     6841 tttggtaaac attatcggaa aggtattggc tgctaaatta ggtgattatt atcgatacct
     6901 aaattctaac cattatgaag ttcactgata atggcagtat tcaataagtt atttaaaagg
     6961 aggcactcag tgtcagaaga aattaaaaaa gacgatttac aggaagaagt ggaagccact
     7021 gaaactgaag agatagttga ggaagttatt gaagaaattc ctgaaaaatc agagttagaa
     7081 ctcgcaaacg agcgtgctga tgagtttgaa aataaatact tgcgtgctca tgcagaaatg
     7141 cagaatatac agcgtcgctc tagtgaagaa cgtcaacaac ttcaacgcta tcgttctcag
     7201 gatctggcaa aagcaatctt accatcgctt gacaatctag aacgtgcctt ggctgttgaa
     7261 gggttgacag atgatgttaa aaaaggttta gaaatgactc gtgatagcct tattcaagca
     7321 ctaaaggaag aaggcgtaga agaagtagaa gtcgactcat ttgaccacaa ctttcatatg
     7381 gcagttcaga ccctcccagc cgatgatgaa catccagccg atagcattgc agaagtcttt
     7441 caaaagggat ataaattgca cgagcgtctg ctaagaccag cgatggtagt tgtctataac
     7501 taaggtaaat gagttttcgt ttttgtccgt aatgacagta aactagatag caagttagaa
     7561 gctattcagc ttgctgatta aactatagtg attgcttaga attggaagta aaataattcg
     7621 agtgcttact aagataaatt gaaataaaaa gtaataaagt attataaaat aagaggtatt
     7681 aacatgtcta aaattattgg tattgactta ggtacaacaa actcagcagt agcagttctt
     7741 gaagggactg aatcaaaaat cattgctaac ccagaaggca atcgtacaac tccttcagta
     7801 gtatcattca aaaatggtga gattatcgtg ggtgatgctg caaaacgtca agcggtaaca
     7861 aatccagata ctgttatctc tatcaaatca aagatgggaa cttctgaaaa agtttctgca
     7921 aatggtaaag aatatactcc tcaagaaatt tcagcaatga ttcttcaata ccttaaaggt
     7981 tatgctgaag actatcttgg agaaaaagta gaaaaagcag ttattactgt tccagcttac
     8041 ttcaacgatg cacaacgtca ggcaactaaa gacgctggta aaattgcagg tcttgaagta
     8101 gaacgtatcg ttaacgaacc aacagcagcc gcacttgctt atggtatgga caagactgac
     8161 aaggatgaaa aaatcttagt ttttgacctt ggtggtggta catttgacgt atcaatcctt
     8221 gaattaggtg atggtgtctt cgacgttctt gcaacagcag gtgataacaa acttggtggt
     8281 gacgactttg accagaaaat tattgatttc ttggtagaag aattcaagaa agaaaatggt
     8341 attgatcttt ctcaagacaa aatggctctt caacgcttga aagatgctgc tgaaaaagct
     8401 aaaaaagacc tttcaggtgt aactcaaact caaatttcat taccgttcat cactgctggt
     8461 tctgctggtc ctcttcactt ggagatgagc ttatcacgtg ctaaatttga cgatctcact
     8521 cgtgaccttg ttgaacgtac gaaaactcca gttcgtcaag ctctttcaga tgcaggcttg
     8581 tcattgtcag aaattgatga agttatcctc gttggtggat caacacgtat cccagcagtt
     8641 gttgaagctg taaaagctga aactggtaaa gaaccaaata aatctgttaa ccctgatgaa
     8701 gtggttgcca tgggtgctgc tatccaaggt ggtgttatca ctggggatgt gaaagacgtt
     8761 gtacttcttg acgtaacacc attgtcactt ggtattgaaa caatgggtgg tgtcttcact
     8821 aaattgatcg accgcaacac aactatccca acatctaaat cacaagtctt ctcaacagca
     8881 gcagacaacc aaccagccgt tgatatccat gttcttcaag gtgaacgccc aatggcagca
     8941 gataacaaaa cactcggtcg cttccaattg actgatatcc cagctgcacc tcgtggaatc
     9001 ccacaaattg aagtaacatt tgatatcgat aaaaatggta ttgtatctgt taaagctaaa
     9061 gatctcggta ctcaaaaaga acaacacatt gttatccaat ctaattcagg attaactgat
     9121 gaagaaattg ataaaatgat gaaagatgct gaagcaaatg ctgaagcaga tgcaaaacgt
     9181 aaagaagaag ttgatcttaa aaatgaagtt gaccaagcca tctttgcaac agaaaaaact
     9241 attaaagaaa ctgaaggtaa aggctttgat acagaacgcg atgcagcgca atcagcactt
     9301 gatgagttga aaaaagctca agaatcaggt aaccttgacg acatgaaagc taaacttgaa
     9361 gctcttaacg aaaaagcaca agctcttgca gttaaacttt acgaacaagc agctgcagca
     9421 caacaagcag ctcaaggggc tgaaggtgca caatcagctg attcatcaag caagggtgat
     9481 gatgttgtag atggcgaatt cactgagaaa taattattaa tattgttcag attcatttga
     9541 atataagcat gaaaactata ctagcatagt aaagttcttc gtgatagggg ttgctcaata
     9601 atctagataa gtttcagatt acataagcta atttcgctat cactaaataa aaacatatta
     9661 ataataaata ggcggggcgc ctcgctccgt ctgttttatt aagtgtcata tatatgttaa
     9721 ctatttagag ctgtaactgg gcaagaataa ttgttaatct cttcaagtgt agtatatgaa
     9781 caaaatataa aggattagat aatgaacaat acagaatttt atgatcgtct tggcgtttca
     9841 aaagatgctt ctcaggacga aataaaaaaa gcttaccgta ggatgtctaa gaaataccat
     9901 ccagatatta ataaggaagc gggagcagag gaaaaatata aagaggttca agaagcttat
     9961 gaaaccttga gtgatacaca aaaacgtgct gcctatgatc aatatggcgc tgctggggca
    10021 aatggcggtt ttggtggctt cgatggtggt ggctttggtg gttttgaaga catcttctca
    10081 agcttttttg gtggaggagg gatgcgcaat cctaatgcac cacgtcaagg agatgaccta
    10141 caatatcgtg ttaatttaag ttttgaagaa gctatctttg gagctgaaaa agaagtatct
    10201 tataatcggg agtcatcatg tcatacttgt tcaggaagtg gagctaagcc aggtactagc
    10261 cctgtgactt gtcaaaagtg tcatggttcg ggtgtgatta atgttgatac acaaacacct
    10321 ctaggaacaa tgcgtcgcca agtgacttgt gatgtttgtc agggtagtgg tcaagaaatc
    10381 aaagagaaat gtccaacatg tcatggaact ggtcatgaga agaagacgca taaagtatca
    10441 gttaaaatac ctgcaggagt tgaaactggg caacaaattc gtttaacagg acaaggagaa
    10501 gctggcttca atggtggtcc ttatggtgat ctctttgtta ttattaacgt tctcccaagc
    10561 caacagtttg agcgtaatgg atcaacaatt tattatactt tgaatattgg ctttgtccaa
    10621 gctgctttgg gtgatacaat tgatatacct actgtccatg gagcggtaga aatgtcaatt
    10681 cctgctggta ctcagacagg aaaaacgttc cgtcttcgcg gaaaaggtgc ccctaaactt
    10741 cgagggggtg gacaaggtga tcagcatgtt actgttaaca tcgtaactcc tactaaattg
    10801 aatgatgctc aaaaagaggc attgcacgct tttgcagagg caagtggaga taagatggta
    10861 catccgaaga agaaaggttt ctttgataaa gtcaaagatg ctttagatgt tgattaaaaa
    10921 ataaaagtct agaatcgaaa gtattctaga cttttttgta gttattgcta gagggattgc
    10981 ttgaacctaa attttacagt acaatgaatc taatattgta gggggacaat ttttatggta
    11041 accaaagttg aagagattcg ctcatatttg atagcttcta tacaaaatgg taagttggct
    11101 ccaggagatc gcctaccatc tataagacag ttagctaatc aattttcctg taacaaagat
    11161 acagtgcaac gagttttgat ggaattgcgt tttgataatt atatctatgc aaagcctagg
    11221 tcaggctatt acgtctttga ttctcatcaa gaggaagttg aagaaggggt tagtttacca
    11281 aactctgaga ttgcaaatat agcttatgat gattttagat tgtgtttgaa tgagaccctt
    11341 attggtaggg aagattacct tttcaattat tactatcgtc aagaaggtct tcttgattta
    11401 agtaaagcag tggctaaatt aatggaagaa acaggggtct atgttcccct tgatgatatt
    11461 gttattacgg ctggtactca acaggcatta tttattttga cacaggttac ctttccaaat
    11521 cgaaaatctc gagttttaat agaagaaccg acttatcctc gtatgattga actaatcaaa
    11581 acacaaaatt taccctatga aactattcct cgaggtactc atggaattga ttttcagcgc
    11641 ttagaggaga ttttccagac acaatcaatt aagttttttt atgttatacc tcgcatgcat
    11701 aatcctttgg gaacatccta taatccggta gagatgaaaa gattaataga gatggcagag
    11761 aagtatgatg tttatattgt ggaagatgac tatatgtctg attttgcaag tcagtcacca
    11821 ttacattatt atgatactca cgggcgtgtt atttatctaa aatctttttc aaaggctatt
    11881 ttccctgctt taagattagc tgcgatttgt ttaccacaag ctttaaaatc aacatttatg
    11941 gcttacaaga agttgatgga ttatgatact aatctgattt tacaaaaagc attagcgctt
    12001 tatattgaaa atggccttta tgctaagaat agtcaatatt tgaaatatcg ttatcagaaa
    12061 gaccttgcaa attcaaaatc tattttagct gatcacccta atctaccccc atatagttta
    12121 catcacgata gtgtattatt tgattgttcg aaactcgata acttcaaaat attacggcaa
    12181 tacggcgata ctttggaaaa ttatttttgt caaaaatcgc atcaatctct cttacaagta
    12241 aaaaatgatt cctgcttaaa gcagttcttg ggatcgttgt agtataattg tagggggaca
    12301 gtttaaaata gatattatag ttttcataaa tgaaaggtgg taaaataaac ccatgacaag
    12361 gtataaggca cagatttctt atgatggttc agctttttca ggcttccaaa gacaaccaaa
    12421 ttgtaggaca gttcaagaag aaattgagag aactcttaag cgcctaaata gcgggaacga
    12481 tgtgataatc tatggtgctg gtcgcacaga tgctggtgtt catgcttatg gacaagttat
    12541 tcattttgat ttaccacagg cgcgtgatgt agaaaaatta cgctttggtt tagatacaca
    12601 gtgtccagac gatattgata ttgttaaagt agagcaagtg tctgatgatt ttcattgtcg
    12661 atatgataaa cacattaaaa cttatgagtt tttagtagat atcggtaggc cgaaaaatcc
    12721 tatgatgaga aactatgcaa cacattaccc ttatcctgtc attattgagc ttatgcaaga
    12781 agctattaaa gatttagtag gcacccatga ctttacaggc tttactgctt caggtacgag
    12841 tgtagaaaat aaagtacgta ctatttttga tgcgaaaatt cagtttgaag cgtctaaaaa
    12901 tcttttaatt tttaccttta caggaaatgg ttttttgtat aaacaggtgc gtaatatggt
    12961 tggtaccctg ctaaaaattg gtaatggacg catgcctatt agccaaatca aaacaatttt
    13021 acaggctaaa aatcgggatt tagcaggtcc aactgcggca ggaaatggtt tatatttgaa
    13081 ggagattatt tatgaagacg aggaatgttt tagcaatttc aggaaatgat attttctctg
    13141 gcggcggctt gcatgcagat ttagcaactt acgttgttaa taaattacat ggttttgtgg
    13201 cagtaacatg cttaacagca atgtcagata aagggtttga agtcattcct atagaggcat
    13261 ctatactaaa gcagcaacta gaaagtttaa aagatgtaga gtttgggtct attaaactag
    13321 gattgcttcc caatgtagaa acagcacaag ttgttttgga atttgtaaaa tcaaaacaag
    13381 aatgtccagt agtcttagac ccagtcttag tttgtaaaga aaatcatgat ttagaagtta
    13441 gccaattacg tgagcagcta attgcgtttt tcccatatgc tgatgtgatt actcctaatt
    13501 tagtagagac tcaattattg acgggtcttt caattgaaaa cttagatcaa atgaaaattg
    13561 ctgctgagaa actttacgat atgggggcta aacatgttgt tatcaaaggg ggcaaccgtt
    13621 taaatgctga agaggctaca gacctttact atgatggtga acgttttgaa acctatgttt
    13681 ttcctgtagt agatgccaat aatacaggtg ctggatgtac atttgcgtca agtattgcca
    13741 gccaattagc tatgggtaaa aatgtagaag atgcagtcaa aatgtctaaa ggtttcgtct
    13801 atcaagctat taaagcatcg gataagtacg gagttgtcca acatttttag gagattttaa
    13861 tgaggaaaga gaaaactagt caattggttc aattggcaat tgtaacagct atatctattg
    13921 tattggggat gtttataagt attccgacac caacaggttt tttgacatta ctagatgctg
    13981 gaatattctt tgctgccttt tactttggca aaaaagaagg ggctgtggta ggtgccttag
    14041 ctggtttttt aattgattta ttgaaaggtt atcccaattg gatgttcttt agtttattga
    14101 ttcatggggc gcaagggtat ttagcgggtt taccaggtag aagacgtctt ttaggtctta
    14161 ttagtgcaac tcttgttatg gtattaggtt atgctatagc tagtggattg atgtatggtt
    14221 ggggagctgt tttaccagat attcctggca atattatgca aaatatggtt ggtatggtcg
    14281 ttggttttgc tcttaataag tctctagaaa gagtgaagaa atgatactag attataataa
    14341 gttaaagcag gaaactaaag ctattgtagt tgatattatt gagcgtagtg ctcttaaaaa
    14401 aggtcaaatt tttgtactag gtctatcatc tagtgaggtt tctggtggtt taattggaaa
    14461 aaattctagt tctgaaattg gtgaaattat cgttgaagtt atcctcaaag agttacattc
    14521 acgcggcatc tatttagcag ttcaaggttg tgaacatgtt aatagggcgt tggttgttga
    14581 agcagaattg gctgaacgtc aacagttgga agtcgttaat gttgttccta atctacatgc
    14641 tggcggtagt ggtcaggttg cagcttttaa gttgatgaca tcaccagttg aggttgaaga
    14701 aattgtggca catgctggaa ttgatattgg agatacttct attggaatgc atatcaagag
    14761 agtacaagtt cccttgattc caattagtcg tgagttggga ggggcacacg ttacagcact
    14821 agctagtcgt cctaaattaa ttggcggtgc tagagcagga tatacgagcg atccaatccg
    14881 taaattttaa aataaacctc tgctatgcag aggtttattg tttatctcaa cgagagtggt
    14941 tattggagaa attgtatgtt ggtaagtcaa taccttcttc aagaatagct ttttgataaa
    15001 gtttgtaaaa ttcagcatag atgtcaaact gagcaccgtt ttgcgtgaag atattaatac
    15061 gataaacaaa ttgaccatta gcattggttg taggaccaaa aacagtaggc tctccaacga
    15121 tagcaggatg ttttgaaaca tactcttcat tgatttttgt tacaatatca gaaatctgat
    15181 caaggtttgt atgaacaaat aaaggaatat caatttgagc acgcatattg ctacgagact
    15241 tattgctgac aattgtaata ttacgattag gaatgaagtg taaggttcca tcgaaatcat
    15301 gaatttgtgt ggtgcgtatc ccaacattag taacagtgcc tgatacagca ccaacattaa
    15361 taatatcacc aacatcaaat tggttttcta gcagaataaa aaagccattg acaacatctg
    15421 ataagaagcc ttgagcacca agtccaatag caaccccagc aataccagcc ccagctaata
    15481 aactagaaat tggaacgccc aatatactta aaatccaata gaatagaaag aaataaaggg
    15541 tatagtttaa aacattgtga gaaagtttag ctaatgtttt ttgacgagct actttttgtc
    15601 tagaaaaggc gagtgagcga ttaacagtct tttcgaaaag ataattgatg acttgcctaa
    15661 cgataacgaa tgcaatgatt aataataaga ttgatattag tttggtgaag aaagtaaata
//
LOCUS       DATRQA010000037        15475 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.037, whole genome shotgun sequence.
ACCESSION   DATRQA010000037 DATRQA010000000
VERSION     DATRQA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15475
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.037"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(147..887)
                     /locus_tag="VBD27_000859"
     CDS             complement(147..887)
                     /locus_tag="VBD27_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7024888.1"
                     /translation="MTQAILEIKHLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STFLRSINLLEEPSGGEILYHGHNVLEKGYDLNNYREKLGMVFQSFNLFENLNILENA
                     IVAQTTVLKRERQEAEKIAKENLNAVGMTEQYWKAKPKQLSGGQKQRVAIARALSVNP
                     EAILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFAKEVSDRVIFMDKGI
                     IAEQGTPKQLFENPTQERTKEFLQRFLK"
     gene            complement(897..2447)
                     /locus_tag="VBD27_000860"
     CDS             complement(897..2447)
                     /locus_tag="VBD27_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO7024889.1"
                     /translation="MKKILLSLFTALLITFGGMTSIQADEYLRVGMEAAYAPFNWTQN
                     DNTNGAVPIEGTDQYANGYDVQVAKKLAKKLNKKVVVVKTKWEGLVPALTSGKLDMII
                     AGMSPTEERKKEINFSKPYYISEPTLVVNAEGKYTNAKNISDFKNAKVTAQQGVYLYN
                     LIDQINGVKKEVAMGDFNQLRQAVESGVVDAYVSERPDATSAQTANPKLKMIELHQGF
                     KTSDADTNISVGMRKGDNRINQVNQVLESISRDKQIALMDKMIKEQPSVKKEKNGKPN
                     FFEQMATILKNNGSQFLRGTATTLLISMVGTIVGLFIGLLIGVFRTAPKSDNKLKAAL
                     QKLLGWLLNIYIEVFRGTPMIVQSMVIYYGTAQAFGVSLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFSVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFILTFTVTRILRYIEKRFDSDNYT
                     TGANQLQV"
     gene            2583..4466
                     /locus_tag="VBD27_000861"
     CDS             2583..4466
                     /locus_tag="VBD27_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEO7024890.1"
                     /translation="MKKCFLAICLALSFFMVSVQADEVDYNIPHYEGNLTIHNDNSAD
                     FTEKVTYQFDSSYNGQYVTLGTAGKLPDNFDINNKPQVEVSINGKVRKVSYQIEDLED
                     GYRLKVFNGGEAGDTVKVNVQWKLKNVLFMHKDVGELNWIPISDWDKTLEKVDFWIST
                     DKKVALSRLWGHLGYLKTPPKIRQNNNRYHLTAFNVNKRLEFHGYWDRSYFNLPTNSK
                     NNYKKKIEHQEKIIERHGFILSFLLRILLPSFFIIVTLFISIRVFLFRKKVNKYGQFP
                     KDHHLYEAPEDLSPLELTQSIYSMSFKNFQDEEKKTHLISQEQLIQSILLDLIDRKVL
                     NYDDNLLSLANLDRASDAEIDFIEFAFADSTSLKPDQLFSNYQFSYKETLRELKKQHK
                     ASDLQNQMRRRGSNALSRITRLTRLISKDNINSLRRKGISSPYRKMSSEESKELSRLK
                     RFSYLSPLISFVVIIYTLFLNYFTYFCIYLLLFGVILLLNKIIFMMTRKISNGYIVTE
                     DGASRVYQWTSFRNMLRDIKSFDRSELESIVLWNRILVYATLFGYADRVEKVLRVNQI
                     DIPERFANIDSHRFAISVNQSSNHFSTITEDVSHASNFSVNSGGSSGGFSGGGGGGGG
                     GAF"
     gene            4512..5351
                     /locus_tag="VBD27_000862"
     CDS             4512..5351
                     /locus_tag="VBD27_000862"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEO7024891.1"
                     /translation="MLIIELLKALFLGVVEGVTEWLPVSSTGHLILVQEFMKLNQSKS
                     FVEMFNIVIQLGAIMAVIVIYFKRLNPFQPGKSAREIRLTWQLWLKVVIACIPSILIA
                     LPFDNWFEAHFNFMIPIAIALIFYGFVFIWVEKRNAHLKPQVTELASMSYKTAFLIGC
                     FQVLSIVPGTSRSGATILGAIIIGTSRSVAADFTFFLAIPTMFGYSGLKAVKYFLDGN
                     VLSLDQSLILLVASLTAFVVSLYVIRFLTDYVKRHDFTIFGKYRIVLGSLLILYWLVV
                     HLF"
     gene            5471..6226
                     /gene="mecA"
                     /locus_tag="VBD27_000863"
     CDS             5471..6226
                     /gene="mecA"
                     /locus_tag="VBD27_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEO7024892.1"
                     /translation="MEMKQISETTLKITISMEDLEDRGMELKDFLIPQEKTEEFFYSV
                     MDELDLPENFKNSGMLSFRVTPKKDRIDVFVTKSELSKDLNLEELADLGDISKMSPED
                     FFKTLEQSMLEKGDTDAHAKLAEIENMMDKATQEVVEENVSEEQPEKEVETIGYVHYV
                     FDFDNIEAVVRFSQTIDFPIEASELYKNGKGYHMTILLDLENQPSYFANLMYARMLEH
                     ANVGTKTRAYLKEHSIQLIHDDAISKLQMIEMG"
     gene            6228..7388
                     /locus_tag="VBD27_000864"
     CDS             6228..7388
                     /locus_tag="VBD27_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO7024893.1"
                     /translation="MIPFTIEYIFVLIGAFLLSIILTPIIRVISLKVGAVDKPNARRI
                     NKVPMPSSGGLAIFLSFVVTTLFFMPMAASRHFIEVSYFHYILPVIIGGLVVTTTGFI
                     DDIFELRPRYKMLGIIIAAIIIWKFTHFRFDSFKIPIGGPLLEFGPILTFFLTVLWII
                     SITNAINLIDGLDGLVSGVSIISLATMAVVSYFFLPKIDFFLTLTIVILIASIVGFFP
                     YNYHPAIIYLGDAGALFIGFMIGVLSLQGLKNSTAVAVITPVIILGVPILDTAVAIVR
                     RKLSGKKISEADKMHLHHRLLSMGFTHRGAVLVVYGIAIIFSLIALLLNVSSRIGGIF
                     LLLALLLVMEIFIEGLNIWGENRTPLFNLLKFIGNSDYRQSVIAKYSDKHQK"
     gene            7553..8323
                     /gene="sufC"
                     /locus_tag="VBD27_000865"
     CDS             7553..8323
                     /gene="sufC"
                     /locus_tag="VBD27_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO7024894.1"
                     /translation="MSVLEIKNLHVSIEDKEILKGLNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTAGEILFDGEDILELEVDERARLGLFLAMQYPSEVPGITNAEFIR
                     AAMNAGKADDGKISIRQFITKLDEKMELLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGEGFGAMIITHYQRLLNYITPDKVHV
                     MMDGKVVLSGGPELAVRLEKEGYAQIAEELGLEYKEEV"
     gene            8360..9622
                     /gene="sufD"
                     /locus_tag="VBD27_000866"
     CDS             8360..9622
                     /gene="sufD"
                     /locus_tag="VBD27_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO7024895.1"
                     /translation="MSKEAILNFLQAKGEPTWLQELRLKAFEKIEELELPVIERVKFH
                     RWNLGDGTILENDYTANIPDFTELGNNPKLVQIGTQTVLEQVPMELIEKGVVFTDFYS
                     ALEEIPEVIERYFGKARPFEEDRLAAYHTAYFNSGAVLYIPDNVEITQPIEGLFYQDS
                     QSKVPFNKHILLIVGKNAKVSYLERFESIGDGTERTSANISVEVIAQAGSQIKFASID
                     RLGENVTTFISRRGRHSSDATIDWALGVMNEGNVVADFDSDLIGDGSHANLKVVAASS
                     GRQVQGIDTRVTNYGCNSVGHILQHGVILERGTLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENDVTAGHAASIGQVDPEDLYYLMSRGLNQKTAEQLVIRGF
                     LGTVIAEIPVKEVRDEMIAVIDTKLEKR"
     gene            9624..10856
                     /locus_tag="VBD27_000867"
     CDS             9624..10856
                     /locus_tag="VBD27_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7024896.1"
                     /translation="MTLLDSYKLKQDFPILNQLVNDEPLIYLDNAATTQKPNQVLETL
                     RDYYQNDNANVHRGVHTLAERATAQYETAREKARQFLNAKSSKEILFTRGTTTGLNWV
                     AKFAESILERGDEVLISIMEHHSNIIPWQQACERTGAKLVYAYLKDGSLDLEDFYNKL
                     SSKTKFVSLAHISNVLGCITPVKAIAERVHQVGAYMVVDGAQSAPHMAIDVQDLDCDF
                     FALSGHKMLGPTGIGVLYGKESILDKMPPVEFGGEMIDFVYEHSATWKELPWKFEAGT
                     PNIAGAIAFGEALDYLTDVGMDEIHQYEQSLVSYVLPKLQAIDGLTIYGPSDAESHVG
                     VIAFNLEGLHPHDVATAMDYEGVAVRAGHHCAQPLINHLGIHSAVRASFYFYNTKEDC
                     DKLVDAIQKTKEFFNGTL"
     gene            10843..11286
                     /locus_tag="VBD27_000868"
     CDS             10843..11286
                     /locus_tag="VBD27_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO7024897.1"
                     /translation="MALSKLDNLYMAVVADHSKHPHHHGFLEGVEQVQLNNPTCGDVI
                     SLSVKFDGNIISDIAFAGNGCTISTASSSMMTDAVIGKTKEEALQLADVFSKMVQGNQ
                     NPKQEKLGDAEFLAGVSKFPQRIKCATLSWNALRKAIERDNQAEL"
     gene            11386..12804
                     /gene="sufB"
                     /locus_tag="VBD27_000869"
     CDS             11386..12804
                     /gene="sufB"
                     /locus_tag="VBD27_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO7024898.1"
                     /translation="MSEINEKVEPQPIDLGEYKFGFHDDVKPIYSTGKGLNEAVIREL
                     SAAKGEPEWMLDFRLKSLETFNKMPMQTWGADLSDIDFDDIIYYQKASDKPARDWDDV
                     PEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKLGIVFTDTDSALKEY
                     PELFKKYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRATAKKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANKGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKDSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(12875..14062)
                     /locus_tag="VBD27_000870"
     CDS             complement(12875..14062)
                     /locus_tag="VBD27_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO7024899.1"
                     /translation="MPKLIVSFLCILLSLTCVNSVQAEEHKDIMQITREAGYDVKDIN
                     KPKASIVIDNKGHILWEDNADLERDPASMSKMFTLYLLFEDLAKGKTNLNTTVTATET
                     DQAISKIYEISNNNIHAGVAYPIRELITMTAVPSSNVATIMIANHLSQNNPDAFIKRI
                     NETAKKLGMTKTHFYNPSGAVASAFNGLYSPKEYDNNATNVTTARDLSILTYHFLKKY
                     PDILNYTKYPEVKAMVGTPYEETFTTYNYSTPGAKFGLEGVDGLKTGSSPSAAFNALV
                     TAKRQNTRLITVVLGVGDWSDQDGEYYRHPFVNALVEKGFKDAKNISSKTPVLKAVKP
                     KKEVTKTKTKSIQEQPQTKEQWWTKTDQFIQSHFVSILIVLGTIASLCLLAGIVLLIK
                     RSR"
     gene            complement(14215..15450)
                     /locus_tag="VBD27_000871"
     CDS             complement(14215..15450)
                     /locus_tag="VBD27_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO7024900.1"
                     /translation="MKKIITSILLLSCIFFMPTISAESFNASAKHALAVDLDSGKILY
                     EKDANKPAAIASLTKIMTVYMVYKEIDNGNLKWNTKVNISDYPYQLTRESDASNVPLE
                     KRRYTVKQLVDAAMISSANSAAIALAEHISGTESKFVDKMTAQLEKWGIHDSHLVNAS
                     GLNNSMLGNHIYPKSSQNDENKMSARDIAIAAYHLVNEYPSILKITSKSVAKFDKDIM
                     HSYNYMLPDMPVFRPGITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKY
                     ARFTATNSLLNYITNTYEPNLVLAKGAAYKGKEASVRDGKEQSVIAVAKNDLKVVQKK
                     NITKQNQLKINFKKELTAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIER
                     SFFLKVWWNHFVRYVNEKL"
BASE COUNT         4837 a         2477 c         3047 g         5114 t
ORIGIN      
        1 actgtctaat ttgatctata gttgaggcta gtcgtagatt tcatcaaaga tgtggtaata
       61 aaaaatcccc tccagttcca ttgtaatgat gacaatgaca aaacctgtag gggtataaat
      121 agtctatatt taaatgtgga taaattttat tttaagaagc gttgtagaaa ttccttagtt
      181 ctttcttgtg ttggattttc gaaaagttgc ttaggagtac cctgctctgc aatgattcct
      241 ttgtccatga aaataacgcg gtctgatact tctttagcaa attccatctc gtgtgtcaca
      301 attatcattg ttaatccaga tttagctaga tcttgcattg ttttaaggac ctcaccaacc
      361 atttctggat ccaaggctga agttggttca tcaaaaagta tagcttctgg gttaacagaa
      421 agtgctcggg cgattgcgac acgttgcttt tgcccacctg ataattgttt tggtttagct
      481 ttccaatatt gctcagtcat gccaacagca tttaagtttt ctttagcaat cttttcagct
      541 tcttggcgtt cacgttttag aacagtagtc tgtgcaacaa tagcgttctc taagatgttt
      601 aaattctcaa agaggttaaa agattgaaag accatcccaa gtttttcacg ataattattt
      661 aaatcatagc ctttttcaag aacattgtga ccgtgatata agatttctcc tccacttggt
      721 tcttctaaga ggttaataga acgtaggaaa gttgattttc ctgatccaga agaaccaatg
      781 atagaaataa cttcgccttt gttgaccgaa agcgaaatgt cttttaacac ttcattgctt
      841 ccgtaagatt ttttcaaatg tttaatttct aaaatagctt gtgtcatacc aacctcctag
      901 acttgtaatt gattcgcacc ggttgtgtaa ttatctgaat caaaacgttt ttcaatgtaa
      961 cgtaagatac gtgttacggt gaaggttaag atgaagtaaa taatcgcaat aatggtgaat
     1021 gtttggaaat attggtaggt ttgtgttgca acagtattac cagagaagta gagttcgaca
     1081 acagagataa cgttcagtac agaggtatct ttgatattga taacaaactc attaccagta
     1141 gctgggagta tgttacgtac aacttgtggt agaacaattt ttcgcatggt ttgtccatgg
     1201 gtgaaaccaa gtgcagttgc ggcttcaaat tgtcctttat caacagagaa aataccacca
     1261 cgtacaattt cgctcatata agcaccagta ttgattgaaa caataaatat agctgctagt
     1321 gtacggtcca gcgaaactcc gaaagcttgt gctgttccgt agtagataac cattgattgt
     1381 acaatcatag gtgtcccgcg gaagacctca atatagatgt taagtaacca acctaataat
     1441 ttttgtaagg cagcctttaa cttattgtca gattttggtg ctgtacggaa aacaccgatg
     1501 agaaggccga taaataatcc gacgatagtt cctaccatag aaattaggag ggttgtagct
     1561 gttcctctga ggaattgaga accattgttt tttaaaatag ttgccatctg ttcaaagaag
     1621 tttggttttc catttttttc ttttttaacg gatggttgtt ctttaatcat cttatccatc
     1681 aaggcgattt gtttatctct agaaatgctt tcgagaactt gattgacttg attaatacga
     1741 ttgtcacctt tacgcatccc aacggaaata tttgtatcgg catcagatgt tttaaaccct
     1801 tggtgcagtt caatcatttt taatttagga ttagcagttt gagcggaagt tgcatctgga
     1861 cgttctgaaa cataggcgtc tacaacacca gattctacag cttgtcttaa ttgattaaag
     1921 tcccccatgg caacttcttt tttgacacca ttgatttgat caatgaggtt gtataagtag
     1981 actccttgtt gggcagttac tttagcattt ttgaaatcac taatattttt agcattagta
     2041 tattttcctt cagcgttaac aactagagta ggttctgaaa tgtagtaagg ctttgaaaaa
     2101 ttaatttctt ttttacgttc ttcggtagga ctcattcctg caataatcat atcgagtttt
     2161 ccagaagtta gtgctggaac aaggccttcc cattttgttt tgacaacaac aactttttta
     2221 tttaactttt tagctaactt tttagcgact tgtacatcat aaccattagc atattgatca
     2281 gttccttcaa taggaactgc accatttgtg ttatcatttt gtgtccagtt gaaaggcgca
     2341 taggctgcct ccataccgac acgtagatat tcatctgctt gtatacttgt catacctcca
     2401 aaggtgataa gcaaagcagt gaataaactt agtaatattt ttttcattca acagcctccg
     2461 ataagttact tcctcctatt ttaccgaaaa acaaggtcga tttcaatatc attatcttat
     2521 tgaaagcgat taattgtatt tttatgttaa aatggtatac aaacaaagga gggaggatga
     2581 taatgaagaa atgttttttg gctatttgtt tagctcttag tttttttatg gtttcagttc
     2641 aagcagatga ggtggactat aacattcctc attatgaggg taatctaact attcacaatg
     2701 ataatagtgc tgattttaca gagaaggtta cttaccaatt tgattcgtcc tataatggac
     2761 agtatgtcac gttaggtacg gcgggtaagt tacctgacaa ttttgatatt aataataagc
     2821 cacaggttga agtttcaatt aatggtaaag taaggaaagt tagttaccag atagaagatt
     2881 tggaggatgg ctaccgtttg aaagtgttta atggtggtga agcaggtgat actgttaaag
     2941 tcaatgttca gtggaaacta aaaaatgttc tatttatgca taaggatgtt ggtgaactta
     3001 actggattcc tattagcgac tgggataaaa cgttagagaa agtagatttt tggatatcaa
     3061 ctgacaaaaa ggttgctctt tctcgtcttt gggggcactt gggttatctt aaaactcctc
     3121 ctaaaataag acaaaataat aatcgttacc atttgacagc ttttaatgta aacaaacgat
     3181 tagaatttca tggttattgg gatagatctt attttaatct acctacaaac agtaaaaata
     3241 attacaagaa aaaaattgaa catcaagaga agataataga gcgtcatggt tttatcctaa
     3301 gtttcttgtt aaggatatta ttaccttcat tctttattat tgtgacacta ttcatctcaa
     3361 ttagggtgtt cctgtttaga aaaaaagtta ataaatacgg gcaattccct aaggatcatc
     3421 atttatatga agcacctgag gacctttcac cattagagtt aactcaaagc atttatagta
     3481 tgagctttaa aaattttcaa gatgaggaga agaaaactca ccttatcagt caagaacaac
     3541 tcatacagtc aattctatta gacttgattg atagaaaagt attgaattat gatgataact
     3601 tgttatctct agctaactta gatagagctt ctgatgcaga aatagatttt atagagtttg
     3661 cttttgcgga ttctacgagt ttgaagccag atcaactctt ttctaattac caatttagtt
     3721 ataaagaaac actacgtgaa ctgaaaaagc agcacaaggc ttcagatctg caaaatcaaa
     3781 tgagacgccg aggaagtaat gccttatcaa gaattacgcg tctcacaagg ttgatttcta
     3841 aagacaatat aaactctctt agaagaaagg gaatttcatc cccttatcgt aaaatgtctt
     3901 cagaagagtc taaagaatta tctaggttaa aaagattcag ttacctatca cctcttattt
     3961 cttttgttgt tataatttat acgctttttt taaattattt tacctatttc tgtatctatc
     4021 tcttattgtt tggtgttatc ctgttgttga ataaaatcat ttttatgatg acaagaaaaa
     4081 taagtaacgg ttatattgta actgaagatg gagcaagtcg tgtctaccaa tggactagtt
     4141 ttaggaacat gctaagggat atcaaatcgt ttgatcgttc agagttagaa agtatcgtat
     4201 tatggaatcg aatattggtt tacgctactt tattcggcta cgctgaccgt gttgagaaag
     4261 tactcagagt gaaccaaata gatattccag aaagatttgc aaacattgat agtcatcgat
     4321 ttgcgatttc agtcaatcaa tctagtaatc atttttcaac gataactgaa gatgttagtc
     4381 acgcttctaa ttttagtgtt aattcaggcg gttcttcagg tggtttctca ggcggcggag
     4441 gcggcggagg tggcggtgcc ttctaatttt ccaaaagtat ggtataataa agaaaaattt
     4501 atggagttat tatgttaata attgaattac tcaaagccct atttttagga gtcgttgagg
     4561 gagtgacaga atggttacct gtttctagta ctggccattt aatcttggtt caagagttta
     4621 tgaagttgaa ccaaagcaaa tcctttgttg aaatgtttaa tattgttata caacttgggg
     4681 caattatggc agttattgtc atttatttta aacgccttaa cccatttcag ccaggtaaat
     4741 ctgctagaga aattcgttta acttggcaat tatggttaaa agtagtgata gcttgtattc
     4801 catctatttt aattgcttta ccatttgata attggtttga agctcatttt aatttcatga
     4861 ttccgattgc aatagcccta atcttttatg gttttgtctt catatgggtt gaaaaacgta
     4921 atgcacacct caaaccacag gtaaccgaat tggcaagtat gtcttacaag acagctttct
     4981 tgattggatg tttccaggtt ctcagtattg ttccgggaac cagtcgttct ggagctacta
     5041 ttttaggagc aattattatt ggaactagtc gttcggtcgc tgctgacttt actttcttcc
     5101 ttgccatccc aactatgttt ggttatagtg gacttaaggc ggttaaatat tttttagatg
     5161 gtaacgtctt gagtttagac caatctttaa tacttttagt agcaagtctg acagctttcg
     5221 tagttagttt atatgttatt cgtttcttga cagactatgt caaacgacac gatttcacca
     5281 tctttggtaa gtatcgtata gtcttaggaa gtttactcat cctctactgg ttagttgttc
     5341 atttattcta aaatataaca taaaaagctg agtattcctc agctttttag cgtgttaggc
     5401 tgtgaaaaat tgctaaaaaa tagctttttc ggtataataa gaaaactgaa tactcgtatg
     5461 aggtgatgat atggaaatga aacaaatcag tgaaacaaca ctgaaaatta caattagtat
     5521 ggaagattta gaagatcgtg gtatggagct gaaagatttc ctaatccctc aggagaagac
     5581 tgaggaattt ttctattctg tcatggatga attagacttg ccagaaaact ttaaaaatag
     5641 tggtatgtta agttttcgag taacacctaa aaaagatcgc attgatgttt ttgttacaaa
     5701 gtctgaatta agtaaagatt taaatttaga agaattagca gatttgggtg acatttcaaa
     5761 aatgtctcca gaagactttt ttaaaacctt ggaacaatcg atgttggaaa aaggggatac
     5821 ggatgcccat gccaaattag cagaaattga aaatatgatg gataaagcaa ctcaagaagt
     5881 agttgaggaa aatgtttctg aagaacaacc tgaaaaggaa gtagaaacga ttggatatgt
     5941 tcactatgtc tttgattttg ataatattga agctgtagtt cgattttcac aaacgattga
     6001 ttttccaata gaagcttctg aactctataa aaatggtaaa ggctatcata tgacaatttt
     6061 gctagacttg gaaaaccaac caagctactt tgctaatttg atgtatgctc gaatgcttga
     6121 acatgctaat gtgggcacaa aaacacgagc ttacttgaaa gagcatagta ttcaattgat
     6181 tcatgatgat gctatttcaa aattacaaat gattgagatg ggataaaatg atacctttta
     6241 caattgagta tatttttgta ttgattggag catttttatt atcaatcatt ctaactccta
     6301 ttattcgagt tatatctttg aaggtaggag cggttgataa accgaacgct cgaagaatca
     6361 acaaagtacc aatgccaagt agtggtggtt tagcaatatt tctctccttt gttgtaacaa
     6421 cattattttt catgccaatg gcggccagta gacactttat agaagtctct tattttcact
     6481 acattttgcc tgtaattatt ggcggtctcg tagttacaac aacaggattt atagatgaca
     6541 tctttgaatt aagacctcgt tataaaatgt tgggaattat tattgcagct attatcatat
     6601 ggaaatttac acattttaga tttgatagtt ttaagattcc aataggtggt ccactcttgg
     6661 agtttggtcc tattttaact ttctttttga cagttttatg gattatttca atcactaatg
     6721 ctatcaatct gattgatggt ctagatggtc tagttagtgg tgtttcaatc atcagtctag
     6781 caacgatggc agtggtttct tatttctttt tacctaaaat agattttttt ctcacgctaa
     6841 ctattgttat tttaatcgcc tcaatagttg gttttttccc ttataattat catccagcca
     6901 ttatctacct aggtgatgct ggtgccttgt ttataggatt tatgattggt gtattatcgc
     6961 ttcaaggttt aaaaaactcc acagctgttg cagtgataac acctgtcatt attttaggag
     7021 ttcctatttt ggatacagct gttgctattg tgaggcgtaa attatcaggg aaaaagattt
     7081 ctgaagctga taagatgcac ctgcatcacc gattgctatc tatgggattt acacatagag
     7141 gagcagtttt agttgtttat ggtattgcaa ttatcttttc gcttatcgct ttgcttttaa
     7201 atgtttctag tcgtattggg ggaatttttc ttctattggc tctactatta gtaatggaaa
     7261 tttttataga aggtttaaat atttggggtg agaatcggac accgctattt aatctcttaa
     7321 agttcattgg caacagtgat tacagacaaa gtgttattgc taaatatagc gataagcatc
     7381 aaaaataaaa gcctgaaaag gcttttttag ttttaatcat ttagatagca gtcacacctc
     7441 atttttgcta taattattta gaatataata tcgggtgagt gccctataat atcttcttta
     7501 aaccatagag aaatatcaca tattctctgt taaccaagaa aaggagtgta caatgtctgt
     7561 acttgaaatc aaaaatcttc atgtttctat tgaagataaa gaaattttaa aaggtcttaa
     7621 tttaacgctg aaaacaggag aaattgctgc tatcatgggt ccaaatggta ctgggaagtc
     7681 gacactttca gcagctatta tgggaaatcc taattatgaa gttacagcag gtgaaattct
     7741 ttttgatggt gaagatattt tagagctaga agtggatgaa cgtgctcgtt tgggtctatt
     7801 tcttgcgatg caataccctt cagaggttcc agggattact aatgctgaat ttatccgagc
     7861 agctatgaat gcgggaaagg ctgatgatgg taaaatttcc atccgccaat ttattactaa
     7921 gttagatgaa aagatggaac tacttggcat gaaagaagaa atggcggaac gttatcttaa
     7981 cgaaggtttt tcaggtggtg agaagaagcg caatgaaatt ttacaactcc taatgttaga
     8041 gcctaagttt gccctcttgg atgagattga ctctggcctg gatattgatg ctcttaaagt
     8101 ggtatcaaag ggagtcaatg aaatgcgtgg tgaaggtttt ggtgctatga ttattacaca
     8161 ctatcaacgc cttttgaatt acattacacc agataaagta cacgttatga tggacgggaa
     8221 ggtagtattg tcaggaggac cagaattagc ggttcgcctt gagaaagaag gttatgctca
     8281 aatcgctgaa gagcttggtt tagagtataa agaagaagtc taatttgtca cttcaactag
     8341 aagagaaaag gagttacaaa tgtctaaaga agctatatta aattttttac aagccaaagg
     8401 tgagcctact tggttacaag aattacgact aaaggctttt gaaaaaatag aagaactaga
     8461 attaccagta attgaacgtg taaaatttca tagatggaat ttaggtgatg gaactatttt
     8521 agagaatgac tatacagcta acattccaga tttcacagag ttaggaaaca atcccaagtt
     8581 ggttcaaatt gggacccaaa ctgtccttga acaagttcct atggaattaa tagagaaagg
     8641 cgttgtcttt acagatttct attcggcttt ggaagaaatt ccagaagtca tagagcgcta
     8701 ctttgggaaa gcacgtcctt ttgaagaaga tagacttgct gcctatcaca cagcttactt
     8761 caacagtggt gcagtgcttt atattccaga taatgttgaa attactcaac caatcgaagg
     8821 tcttttttat caagatagtc aatctaaagt accatttaat aagcatatcc ttcttattgt
     8881 tggaaaaaat gcaaaagtta gttacttaga gcgatttgag tcaattggtg atgggacaga
     8941 acgaacaagt gctaatattt ctgtagaagt aattgcccaa gcaggtagcc aaattaaatt
     9001 tgcctcaatt gatcgcctcg gagaaaatgt gacaacgttt ataagtcgtc gtggtcgtca
     9061 cagtagcgat gcaacaatag attgggccct tggggtgatg aatgagggca atgttgttgc
     9121 tgactttgat tctgatttaa ttggtgatgg aagccacgct aatttaaaag tagttgctgc
     9181 ttcgtcaggg cgtcaagttc aaggaattga tacgcgtgtt acaaactacg gctgtaattc
     9241 agtaggacat attttacagc atggggttat tttagagcgt gggactctta cttttaacgg
     9301 tatcggacat attataaaag gtgcaaaggg agcagacgcc caacaagaaa gccgagttct
     9361 catgttatca gacaaagcac gttcagatgc taatcctatc cttttgattg atgaaaatga
     9421 tgttacagct ggtcacgcag cctcaattgg tcaggttgac ccagaggatt tatattattt
     9481 gatgagccgt ggcttgaacc aaaaaacagc agagcaatta gttattcgtg gatttttagg
     9541 aacagtcatt gctgaaatac cagtaaaaga agttcgcgat gaaatgattg ccgttattga
     9601 tacaaaacta gaaaaacgtt aatatgacat tactagatag ttataaacta aagcaagatt
     9661 tcccaatttt aaatcaatta gttaatgatg agccgcttat ctatttagat aatgcagcaa
     9721 cgacacaaaa accgaatcaa gttttagaaa ctttgcgaga ttactaccaa aatgacaatg
     9781 caaatgtcca tcgtggtgtt catactttag cagaacgagc gactgctcag tacgagactg
     9841 ctcgtgaaaa agcaaggcag tttttaaatg ctaaatcaag taaagaaatt ttattcacac
     9901 gtggcaccac aacaggatta aattgggtag ctaagtttgc cgaaagtatt ttagaacgag
     9961 gtgatgaggt acttatttcc attatggagc atcattctaa tattatccca tggcaacaag
    10021 cttgcgagag aactggtgct aaattggttt atgcctatct aaaagatggt tctttagatt
    10081 tagaagattt ctataataaa ttatcctcta aaactaaatt tgttagtttg gcacatatct
    10141 caaatgtact tggctgtatt acaccagtaa aagcaatagc agaacgtgtg catcaagttg
    10201 gggcatacat ggttgttgat ggagctcaat cagcgccaca tatggcgatt gatgttcaag
    10261 accttgattg tgatttcttc gccttatctg gccataagat gcttggtcct actggtattg
    10321 gtgttcttta tgggaaagaa agcattttag ataagatgcc acctgtcgaa tttggtggtg
    10381 aaatgattga ttttgtctat gagcatagtg caacatggaa ggaattgccc tggaagtttg
    10441 aagctggaac gcctaatatt gctggagcga tcgcttttgg agaagcttta gattatttaa
    10501 cggatgttgg tatggatgag attcatcaat atgaacaaag tttagtttct tatgtgttgc
    10561 ctaaattaca ggctatcgat ggtttaacta tttacggacc tagtgatgct gaatctcatg
    10621 taggcgttat tgccttcaat ttagaaggtt tacacccaca tgatgtggca actgcaatgg
    10681 attacgaagg agtggctgtt cgtgcaggtc atcattgtgc acaacccctt attaatcatc
    10741 ttggtatcca ttcggcggtt cgagcaagtt tctatttcta taataccaaa gaggattgcg
    10801 acaaattggt agacgctatc caaaaaacta aggagttttt caatggcact ctctaaatta
    10861 gataacctct acatggcggt ggtagctgat cattcaaaac accctcatca tcatggattt
    10921 ttagaaggcg ttgaacaagt gcagttaaac aatcctacat gtggagatgt tatttcctta
    10981 tctgttaaat ttgatgggaa tattatttca gatattgcat ttgctggtaa tggttgtaca
    11041 atttctacgg cttcatcaag catgatgaca gatgcagtta ttgggaaaac aaaagaagaa
    11101 gcattgcagc ttgctgatgt attctccaaa atggttcaag gcaatcaaaa tccaaaacaa
    11161 gagaaacttg gggatgcaga gtttctagct ggggtatcaa aatttccaca acgtatcaaa
    11221 tgtgctacgc tttcgtggaa tgctttaaga aaggctattg agagagataa tcaggcagaa
    11281 ctctgacaac catagttgaa ggaattacat ttaaacaact tttactgaca taaggtagtc
    11341 tagtagaaga atgtcttaaa tttaaatatt aaagaaagga taagtatgtc cgaaataaat
    11401 gaaaaagtag aaccacagcc aatagatctt ggggaatata aatttggatt tcatgacgat
    11461 gtaaagccaa tttattctac gggaaaaggt ctaaatgagg ctgttattcg tgagttatct
    11521 gcagctaagg gtgaacctga gtggatgttg gactttcgtc taaaatcctt ggaaacgttt
    11581 aataaaatgc cgatgcagac ctggggagca gatttatcag atattgattt tgatgatatt
    11641 atttattatc aaaaagcatc tgataaacct gcgcgtgatt gggatgatgt tccagaaaaa
    11701 atcaaagaaa cttttgaaag aattgggatt ccagaagctg aaagagccta tcttgcagga
    11761 gcatcagcac aatatgaatc agaagtagtt tatcacaata tgaaagaaga atatgataag
    11821 ctgggtattg tttttacgga tactgactct gcacttaaag agtacccaga gctattcaaa
    11881 aaatattttg ctaaacttgt ccctccaaca gataataaat tagctgctct gaactctgct
    11941 gtatggtcag gtggaacatt tatttatgtt cctaaaggtg ttaaggtgga tattccactt
    12001 caaacttact tccgtattaa taatgaaaat actggacaat ttgaacgtac tctcattatt
    12061 gttgatgagg gagcaagtgt tcactatgtt gaaggttgta ccgccccaac ttattcttca
    12121 aatagtttac atgcagctat agttgaaatt tttgcacttg atggagctta tatgcgctat
    12181 acgactattc aaaattggtc cgataatgtc tataatttag tgacaaaacg tgctaccgct
    12241 aaaaaagatg caacagttga gtggatagat ggaaatctag gagctaaaac aacaatgaaa
    12301 tacccatcgg tttaccttga tggtgaagga gcacgtggca cgatgttgtc tattgctttt
    12361 gcaaacaaag gacaacacca agatacgggt gcaaagatga ttcataatgc cccccatact
    12421 agttcatcca ttgtctctaa atcaattgct aagggtgggg gaaaagttga ttatcgaggt
    12481 caagtgacat ttaataaaga ttccaaaaaa tcagtgtcac atatagaatg tgacaccata
    12541 ttgatggatg atatttcaaa atcagatacc ataccgttta atgagattca taattcacag
    12601 gttgctttag agcatgaagc aaaggtgtct aagatttctg aagagcaact gtactacttg
    12661 atgagtcgag gtttatctga agctgaagca acagaaatga ttgttatggg gtttgttgag
    12721 ccctttacga aagaattacc aatggaatat gcggtagagt taaatcgttt aatttcctat
    12781 gaaatggaag gttcagttgg ttaattgaag atttcaagag ataataaaaa gcccctatgt
    12841 tattgatgaa ggggctttta tattagcaca gttattatct agagcgcttt ataagtaata
    12901 ctatcccagc taaaagacaa aggctagcga tggtgcccag aacaattaaa atagatacaa
    12961 aatgtgattg gataaattga tctgtttttg tccaccactg ttcttttgtt tgaggctgtt
    13021 cttgaataga tttagttttg gttttagtaa cttctttttt aggtttaacg gcttttaata
    13081 caggagtttt agaagaaata tttttagcgt ctttaaaacc tttttctaca agagcgttga
    13141 caaacggatg acgatagtac tctccgtctt ggtctgacca atcgccaact cctaaaacca
    13201 cagttatcaa gcgagtattc tggcgtttag ctgtaactaa ggcattaaaa gcagcgctag
    13261 ggctagaacc agtttttaag ccatctactc cttctaatcc aaatttagcg ccgggggtag
    13321 agtagttata agttgtaaat gtttcttcat aaggagttcc gaccatggcc ttgacttcag
    13381 gatattttgt atagttcagt atatcagggt attttttaag gaaatgatag gttaaaattg
    13441 atagatcacg tgcagtcgta acgttagtag cattgttatc gtattctttt ggggagtaaa
    13501 gtccattaaa agcactcgct accgccccac tggggttata aaagtgagtt tttgtcatac
    13561 cgagtttctt ggcggtttca ttgattcgtt taataaaggc gtcaggattg ttttgtgata
    13621 agtggttagc aatcataata gttgctacat tagatgacgg gacagccgtc atagtaatca
    13681 gttcacgaat aggataagca accccagcat gaatattgtt attactaatt tcataaatct
    13741 tacttatggc ttggtctgtt tctgttgcag tcactgtggt gttgaggttt gtttttcctt
    13801 tagctaagtc ttcaaatagt aaatataaag taaacatttt agacatgcta gcgggatcac
    13861 gttctaaatc ggcgttatct tcccacaaaa tatgaccttt attgtcaata acgatagacg
    13921 ctttaggttt attaatatct ttaacatcat atccggcttc tcgggtaatt tgcataatat
    13981 ctttatgttc ttcagcttgc acagagttta cacaagtcag ggataataaa atgcagagga
    14041 aagatacgat taatttaggc ataggtaact cctgaaaact ttttattaat attataacaa
    14101 aaaatcacta ataaaaaaga catttcatta gtgattttta attttatata ttctcctaaa
    14161 cctagctaaa gaaactgctg tgcagtaact ccttgtgtca aagggagatt actattaaag
    14221 tttttcgtta acgtagcgca caaaatgatt ccaccacact ttgaggaaga aactgcgctc
    14281 aatactatct tttgccacta aatgaacgct aggttccttt atgagatagc cttttccaac
    14341 cttattaagg tcaacgtaat aagctttccc taggttctct ttttttgtaa taggagcagt
    14401 aagctctttt ttaaagttaa tttttaactg attttgttta gtgatatttt tcttctgtac
    14461 tactttcaaa tcgtttttag caacagcgat gaccgattgt tcttttccgt ctctcacact
    14521 tgcttcttta cctttatatg cagctccttt agctaataca aggttaggtt cgtaggtgtt
    14581 tgtgatatag ttcaagagag agttagttgc tgtaaagcga gcatatttgt ctttatcggc
    14641 cttatcagca tgcataataa cagtgagtag tctcattcca ctttcagtag atgtagcaat
    14701 aaaagattgg ccagctaatt ccgttgtccc agttttcaaa cctgtaatac ctggtctaaa
    14761 gacaggcata tctggtagca tgtagttata agaatgcata atatctttat caaatttagc
    14821 aacggactta ctagtaatct taagaatgga aggatattcg ttgaccaaat ggtaggcagc
    14881 aatagcaata tcacgtgcac tcattttatt ttcgtcgttt tgtgacgatt ttggataaat
    14941 gtgattgcct aacatactat tatttaagcc agaagcattg actaggtggc tatcatgaat
    15001 tccccacttt tccaattgag cagtcatttt atcaacaaat ttactttcag ttcctgaaat
    15061 atgttcagct aaagcaatgg ctgcactgtt agcactagaa atcatggcag cgtccacgag
    15121 ttgtttaaca gtatagcgcc ttttttctaa aggaacatta ctagcatcag attcgcgtgt
    15181 tagttgataa gggtagtcag atatatttac tttggtattc cacttgaggt taccgttatc
    15241 aatttcttta tagaccatat aaacggtcat tattttagtc aaggaagcaa tagcagcggg
    15301 tttgttagca tctttttcat acaagatttt tcctgaatct aaatcaactg ctaaggcatg
    15361 tttagcggaa gcattaaaag attcagcaga gatggttggc ataaaaaaaa tgcaactaag
    15421 taatagaata gaagtaataa tttttttcaa aataacacct ttagaaattt tttaa
//
LOCUS       DATRQA010000038        15369 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.038, whole genome shotgun sequence.
ACCESSION   DATRQA010000038 DATRQA010000000
VERSION     DATRQA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15369
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.038"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            23..1213
                     /locus_tag="VBD27_000872"
     CDS             23..1213
                     /locus_tag="VBD27_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO7024901.1"
                     /translation="MNQPSEKEFKNSLFFKWILNNQAVIALMITFLVFLTIFIFTKIS
                     FMFKPVFDFLAVLILPLVISGLLYYLLKPMVTFLEKRGIKRVTAILSVFTIIILLLIW
                     AMSSFIPMMSNQLRHFMEDLPSYVNKVQMETSSFIDHNPWLKSYKGEISSMLSNISSQ
                     AVSYAEKFSKNVLDWAGNLASTVARVTVATIMAPFILFYLLRDSRNMKNGFLMVLPTK
                     LRQPADRILREMNSQMSGYVQGQIIVAITVGVIFSIMYSIIGLRYGVTLGIIAGVLNM
                     VPYLGSFVAQIPVFILALVAGPVMVVKVAIVFVIEQTLEGRFVSPLVLGNKLSIHPIT
                     IMFILLTSGAMFGVWGVFLSIPIYASIKVVVKELFDWYKAVSGLYTIDVVTEERSEEV
                     KNVE"
     gene            1203..2429
                     /locus_tag="VBD27_000873"
     CDS             1203..2429
                     /locus_tag="VBD27_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024902.1"
                     /translation="MLNSEKMIVSIQNQDLEHANKYFEKALKNDPEEILLELGAYLES
                     IGFLPQAKRLYDQIRPNYPEVAINLAQIVAEDGDIEQAFLYLDYISEDSQEYVSALLV
                     MADLYDMEGLTDVAREKLLLASKLSDDPLVTFGLAEMNLSLEHYQEAIEGYASLDNRE
                     ILETTGVSTYQRIGKSYAIIGKFDAAIEFLEKAVDIEYDDLTVFELATILYDQEEYQK
                     ANLYFKQLDTINPDFDGYEYIYGLSLREEHKSEEALRLVQQGLRKNSFDGQLLLLASQ
                     LSYELHDVPSSESYLKQAEKVSENQDEIVMRLSNLYLEEERFEEVLELDNDDLENILA
                     KWNIAKAHKALEMDDSVDYYQSLYNDLKDNPEFLQDYAYILREFGYLDKAQEVGKAYL
                     KLVPDDIEMSEWVNNI"
     gene            2539..4221
                     /gene="alsS"
                     /locus_tag="VBD27_000874"
     CDS             2539..4221
                     /gene="alsS"
                     /locus_tag="VBD27_000874"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEO7024903.1"
                     /translation="MTESHNQYGADLIVDSLINHDVKYVFGIPGAKIDRVFDTLEDKG
                     PELIVARHEQNATFMAQAVGRITGEPGVVIATSGPGISNLATGLVTATDEGDAVLAIG
                     GQVKRGDLLKRAHQSMNNVAMLEPITKYSAEVHDPNTLSETVANAYRLAKSGKPGASF
                     ISIPQDVTDSPVSVKAIKPLSAPKLGSASVLDINYLAQAINNAVLPVLLLGNGASSEG
                     VTAAVRRLLDAVKLPVVETFQGAGIVSRELEDETFFGRVGLFRNQPGDMLLKRADLVI
                     AIGYDPIEYEARNWNAEISARIIVIDVEQAEIDTYFQPERELIGDMAHTLDLLLPAIK
                     GYELPEGSKEYLKGLRNNIENVSDVKFDRDSAHGLVHPLDLIDVLQDNTTDDMTVTVD
                     VGSHYIWMARYFKSYEARHLLFSNGMQTLGVALPWAISAALLRPNTKVISVSGDGGFL
                     FSAQELETAVRLHLPIVHIIWNDGKYNMVEFQEEMKYGRSSGVDFGPVDFVKYAESFG
                     AKGYRVDSKDSFEETLKQALIDAENGPVLIDVPIDYKDNVTLGETILPDEFY"
     gene            4235..4954
                     /gene="budA"
                     /locus_tag="VBD27_000875"
     CDS             4235..4954
                     /gene="budA"
                     /locus_tag="VBD27_000875"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEO7024904.1"
                     /translation="MSETVKLFQYSTLSSLMAGLYKGSLTIGELLTHGDLGIGTVHMI
                     DGELIVLDGKAYQAIGTDGKAEIIQLSDDVTVPYAAVLPHHIQKQFDINAETDNKDLE
                     EMILKNFEGQNLFKSLKIKGTFSRMHVRMIPKSPQHKRFADIASNQPEFTRENVSGTL
                     VGIWTPELFHGVGVKGFHVHFISDDLTFGGHVMDYSLTQGKVEIGKVDQLDQCFPTQD
                     QEFLKANFDLQKLREDIDLSE"
     gene            complement(5008..6663)
                     /locus_tag="VBD27_000876"
     CDS             complement(5008..6663)
                     /locus_tag="VBD27_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEO7024905.1"
                     /translation="MSFDGFFLHHLTNELQEQIEKGRIQKVNQPFDHELVLTIRNNRR
                     NYKLLLSAHPVFGRIQTTEANFQNPQNPNTFTMIMRKYLQGAVIETIQQIENDRILEI
                     IVSNKNEIGDHIKATLVVEIMGKHSNIILIDKNEHKIIESIKHVGFSQNSYRTILPGS
                     TYIAPPKTKAINPFDISDQTLFELLQTNDLSPKNLQQLFQGLGRDTALELSHCLKDNK
                     LNDFRQFFSREYYPSLTEKSFSAVQFSSSHETFQSLGQLLDYYYQEKAEKDRIAQQAS
                     DLIHRVQSELEKNIKKLAKQQDELLATENAEEFRQKGELLTTYLSMVPNNQDIVVLDN
                     YYTNQTIEISLDRALTPNQNAQRYFKKYQKLKEAVKHLKGIISDTKNTITYLESVETS
                     LNHASMEDINDIREELVETGFIKRRAHDKQHKRKKPEQYLASDGKTIIMVGRNNLQND
                     ELTFKMARKGELWFHAKDIPGSHVLIRDNLNPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLNKPSGTKPGFVTYTGQKTLRVTPTQEKIDSLKLKK"
     gene            7022..8026
                     /locus_tag="VBD27_000877"
     CDS             7022..8026
                     /locus_tag="VBD27_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7024906.1"
                     /translation="MKNKGLIATLILLTILVVGELFYNKSEKHLNLSEKQVVKIGILQ
                     YVTHDALDAIEKGVEDGLAQEGYKGKKVKLTVLNAEADQSKIQAMSKQLVNHHNDILI
                     GIATPSAQGLAASTKDTPIIMGAVSDPLGAKLVTNMKKPTTNVTGLSNVVPTKQTVQL
                     IKDITPNVKRIGILYASSEDNSVSQVTEFTKYAQKAGLEVLKYSVPSTNEIKTSMSVM
                     TKKVDAVFVPQDNTIASAFRTVIVAANQANIPVYSSVDTMVEQGSIASVAQSQYGLGL
                     ETAKQAIKVLRGKPVKDVPVKVIDTGKPSLNLKAAKHLGIKIPKKIMKQAEITVKVDD
                     "
     gene            8039..8902
                     /locus_tag="VBD27_000878"
     CDS             8039..8902
                     /locus_tag="VBD27_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7024907.1"
                     /translation="MIISSVSQGLLWGILGLGIYLTFRILKFPDMTTEGSFPLGGAVC
                     VTLMNQGVNPILATILGMLSGMLAGFVTGLLYTKGKIPTILAGILVMTSCHSIMLMVM
                     KRANLGLNEIQTLKDFLPFSNDLNLLVLGLIAILLAISVLIYFLYTRLGQAYIATGDN
                     PDMAKSFGIDTDKMEMLGLIVSNGLIALSGALVSQQDGYADVSKGIGVIVIGLASIII
                     GEVLYSTGLTLFERLIAIVVGSILYQFLITAVIALGFNTNYLKLFSAIVLGICLMVPV
                     LKTKILKGVRL"
     gene            8902..9663
                     /locus_tag="VBD27_000879"
     CDS             8902..9663
                     /locus_tag="VBD27_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7024908.1"
                     /translation="MMMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEHDFITILGG
                     NGAGKSTLFNVIAGTLMLSSGNIYIMGQDVTNLSAEKRAKYLSRVFQDPKMGTAPRMT
                     VAENLLVAKFRGEKRPLVPRKITNYTEEFQKLIARTGNGLDRHLETPTGLLSGGQRQA
                     LSLLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDALK
                     YGNRVLVMKDGKIVRDLNQAQKNKMAIADYYQLFD"
     gene            9960..11621
                     /locus_tag="VBD27_000880"
     CDS             9960..11621
                     /locus_tag="VBD27_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO7024909.1"
                     /translation="MSDIKIIALGGVRENGKNLYVVEVNDSIFVLDAGLKYPENEQLG
                     VDVVIPNLDYLIENKKRVQGIFLTHGHADAIGALPYIIAEVKAPVFGSPLTIELAKLF
                     VKNSTAVKKFNNFHVIDSETEIEFQDAVISFFKTTHSIPESMGIVIGTKEGNIVYTGD
                     FKFDQAARKYYQTDLARLAEIGRDGVLALLSDSANATSNEQVASEYEVGDEIKSVIED
                     AEGRVIVAAVASNLIRIQQVFDAAAENGRRVVLTGFDIENIVRTAIRMKRIHIADENM
                     IIKPKDMTRYEDNELLILETGRMGEPINGLQKMAIGRHRYVQIKDGDLVFIVTTPSIA
                     KEAVVARVENLIYKAGGSVKLITQNLRVSGHANGRELQLLMNLLKPKYLFPIQGEYRD
                     LSAHAGLAQEVGMSADDIYIVKRGDIMVLEKDGFFHSGSVPAGDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVVITVSKKEKKIISKARVNTRGFVYVKKSRDILRESAELVNTT
                     VEDYLSKDTFDWGELKGKVRDEVSKFLFDQTKRRPAILPVVMEVR"
     gene            11678..12466
                     /locus_tag="VBD27_000881"
     CDS             11678..12466
                     /locus_tag="VBD27_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEO7024910.1"
                     /translation="MAFFNIEYHSKVLGTERQVNVIYPDAFEMSDDKIDDCDIPVLYL
                     LHGMGGNENSWQKRTNIERLLRHTNLIVVMPSTDLAWYTNTKYGLDYFDAIAIELPKV
                     LKRFFPNMSDKREKNFIAGLSMGGYGAYKIALLTNRFSHAASLSGALSFDFDLLFNNG
                     NDNTNYWSGIFGDLNNTDNIERHSLRRYVESFDMKTKFYAWCGYEDFLFEANEVAIDE
                     LRQLGLTIDYSNDHGKHEWYYWNQQLEKVLEWLPVDYVKEERLS"
     gene            12556..13266
                     /locus_tag="VBD27_000882"
     CDS             12556..13266
                     /locus_tag="VBD27_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEO7024911.1"
                     /translation="MRTLFRMIFAIPKFIFRLIWNIIWGIFKTVLVIAIILFGLYYYA
                     NHSQSEFANQLSDIIQTGKTFLNFADTNQLKNSFANLATDNVHHSENTRWDKNQATIY
                     IATRDSELASAYKQAISNWNATGAFVLQTTDNPNADIIAKDYSDAKTQAAGVAETERN
                     ALTNRISKVTVKLNTFYLKNKQFGYDHTRIVNTAEHELGHALGLGHNDQQHSVMQSKG
                     SHYGIQEVDIQTLKNLYA"
     gene            13504..14175
                     /gene="rpiA"
                     /locus_tag="VBD27_000883"
     CDS             13504..14175
                     /gene="rpiA"
                     /locus_tag="VBD27_000883"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO7024912.1"
                     /translation="MDELKKLAGVTAAKYVKNGMIVGLGTGSTAYFFVEEIGRRVKEE
                     GLQVIGVTTSNRTTEQARGLGIPLKSADDIDVIDVTVDGADEVDPDFNGIKGGGGALL
                     MEKIVATPTKEYIWVVDESKLVETLGAFKLPVEVVRYGSERLFRVFKSKGYCPSFRET
                     EGDRFITDMGNYIIDLDLKKIEDPKQLANELDHTVGVVEHGLFNGMVNKVIVASKNGL
                     DILEK"
     gene            14232..>15369
                     /locus_tag="VBD27_000884"
     CDS             14232..>15369
                     /locus_tag="VBD27_000884"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO7024913.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIVSAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADI"
BASE COUNT         4907 a         2399 c         3150 g         4913 t
ORIGIN      
        1 ttcatcttga aaggatatat gaatgaatca accatcagaa aaagaattta aaaatagttt
       61 gttttttaaa tggatcttaa ataatcaagc agttattgct ctcatgatta cctttttggt
      121 atttttaacg atttttattt ttaccaaaat ctcttttatg tttaaacctg tgtttgattt
      181 tcttgctgtg ctgatattgc cgcttgtaat ttctggcttg ctttattacc tattaaaacc
      241 tatggttaca tttttagaga agcggggaat taagcgtgta acagcgatat tatcagtttt
      301 tactattata atccttctgt taatttgggc aatgtctagt tttattccca tgatgagtaa
      361 tcaattacgc cattttatgg aagatctccc ttcatatgtg aataaagtgc aaatggaaac
      421 aagttcgttt atagatcaca acccttggtt aaaatcttat aaaggggaaa tatcgagcat
      481 gttatctaat atcagtagcc aagcggtctc ttatgctgaa aaattttcaa agaatgtttt
      541 agattgggca ggaaatttag ctagtacagt tgcacgtgtg acagtagcaa caatcatggc
      601 tccctttatt ttgttttatc ttttaagaga tagtcgcaac atgaagaatg gtttcttaat
      661 ggttttacca accaaactac gccaaccagc tgatcgtatt ttgcgagaaa tgaatagtca
      721 aatgtcagga tatgtgcaag gacaaatcat tgttgctatt actgttggtg ttattttttc
      781 aataatgtat agtattatag gccttagata tggcgtgaca ttagggatta ttgccggtgt
      841 gttaaatatg gttccctatt tgggaagttt tgtcgcccaa attccagtgt ttatcttagc
      901 gcttgtcgca ggacctgtta tggttgttaa agttgcgatt gtttttgtta ttgagcaaac
      961 tctagaggga cgctttgtct cacctttggt tttaggtaat aaacttagca ttcatccaat
     1021 tacaattatg tttattttat taacctctgg agcgatgttt ggtgtttggg gagtattcct
     1081 cagtattccg atttatgcat ctatcaaagt tgttgttaaa gaattgtttg attggtacaa
     1141 agctgtcagt gggctatata caatagatgt tgttactgaa gaaagaagtg aagaagttaa
     1201 aaatgttgaa tagtgaaaaa atgattgttt ctatccaaaa tcaggattta gaacatgcta
     1261 acaagtattt cgaaaaagcg ttgaaaaatg atcctgagga aatcttattg gaactaggag
     1321 cttatttgga gagtattggt tttttacccc aagcaaaacg tctctatgat caaatccgac
     1381 caaattatcc agaagttgct atcaatttag ctcaaattgt tgccgaagat ggagatattg
     1441 aacaagcatt cttatactta gattatatct cagaagatag tcaagagtat gtttcagccc
     1501 tcctcgttat ggctgactta tatgatatgg aaggcttgac tgatgttgcg cgtgaaaagc
     1561 ttttgctagc cagcaaactt agcgatgacc cattggttac atttggttta gctgaaatga
     1621 accttagttt ggaacattat caagaagcga tagaaggata tgcatcactt gacaatcgag
     1681 aaatcttaga aacgactgga gtttcaactt atcagcgtat tggtaaatcc tacgctatta
     1741 tagggaaatt tgatgcagct atcgaatttt tagaaaaagc cgttgacatt gagtatgatg
     1801 atttaacagt ttttgagtta gcaacaatcc tttatgatca agaagaatat caaaaagcta
     1861 acctttattt taaacagttg gatactatta atccagactt tgatggctat gaatatatct
     1921 atggtttatc tcttcgtgag gagcataaat ctgaagaagc tttacgtcta gttcagcaag
     1981 gtcttcgtaa aaatagtttt gatggtcaac ttctcttact agcttcacaa ttatcgtatg
     2041 aattacatga tgttccttcg tcggagtctt atttgaaaca agctgaaaaa gtttctgaaa
     2101 accaagatga aattgttatg agattgtcaa atttgtatct tgaagaagag cggtttgagg
     2161 aagtcttgga attagacaat gatgatttag aaaatatcct tgcaaaatgg aatatcgcta
     2221 aagcacacaa ggctttagaa atggatgata gtgtagacta ttatcaatct ttgtataatg
     2281 atttaaaaga taatccagaa tttttacaag attatgctta cattttgaga gaatttggtt
     2341 accttgataa ggcacaagaa gtaggaaagg cttatttaaa attagttcca gacgatatag
     2401 agatgtcaga atgggtgaat aacatttaaa taggaaaaca cttacagaaa aaaataaaaa
     2461 attcacaagt aaattttcac aatctgatgt aaaaactgtg atataattga accattcttg
     2521 tgaaaagagg gagataaaat gacagaatca cataatcaat atggggctga cttaattgtc
     2581 gatagcctca ttaatcatga tgtcaaatat gtttttggta tcccaggagc taagattgac
     2641 cgcgtatttg acactctaga agataaaggt ccagagttaa ttgttgctcg tcatgagcag
     2701 aacgcgactt ttatggctca ggctgtcggg cgtattacgg gagaaccagg agttgttatt
     2761 gcaactagtg gacctgggat ctcgaacttg gcaacagggt tagttaccgc gacagatgaa
     2821 ggtgatgctg ttcttgcaat tggtggacag gttaagcgag gagatttatt gaaacgtgct
     2881 catcaatcaa tgaataacgt tgctatgcta gaacctatta caaaatattc tgctgaggtt
     2941 catgacccta atactttgtc agaaactgtt gcaaacgcct atcgtcttgc taaatctggt
     3001 aaaccaggag caagttttat ctctattcct caagacgtta cagacagtcc agtctcagta
     3061 aaagcaatca aacctcttag tgcacctaaa ttgggttcgg catctgtttt agatattaat
     3121 tatttagcgc aagctattaa caatgctgtt ttaccggtac tgttattagg aaatggcgca
     3181 tcttcagagg gagtaactgc tgccgtacgt cgacttcttg atgctgttaa actaccagta
     3241 gtagaaactt tccaaggtgc gggtattgtg tcacgtgagc tcgaggatga aacattcttt
     3301 ggtcgtgtag gcctcttccg taatcaacct ggagatatgc ttttgaagcg tgcagaccta
     3361 gttattgcta tcgggtatga ccctattgaa tatgaggcgc gtaactggaa tgctgaaatt
     3421 tctgctcgta ttatcgttat tgacgttgaa caagcagaaa ttgatacgta tttccaaccc
     3481 gagcgagaac tgattgggga catggcacat acacttgatt tacttttacc agctattaaa
     3541 ggatatgaat tgccagaagg ttcaaaagag tatttgaaag gtcttcgtaa taacattgaa
     3601 aacgttagcg atgttaaatt tgatcgtgac tcagctcatg gacttgttca tcctcttgat
     3661 ctcattgatg tactccaaga taataccacc gatgacatga ctgttacagt tgatgtggga
     3721 agccattata tttggatggc tcgctacttt aaatcatatg aagcgcgtca cttgctcttc
     3781 tcaaacggaa tgcaaacatt aggagttgct ctaccttggg caatttctgc ggctcttttg
     3841 cgtcccaaca caaaagttat ttcagtttct ggtgatggtg gcttcttatt ctcagctcaa
     3901 gagttagaaa ctgcagttcg tcttcatcta ccaattgttc acattatttg gaacgatgga
     3961 aaatataaca tggttgaatt ccaggaagaa atgaagtatg ggcgttcttc aggtgttgac
     4021 tttggaccag ttgattttgt aaaatacgct gaaagtttcg gtgctaaagg ttaccgtgta
     4081 gacagcaaag acagttttga agaaacactc aaacaagctc taatcgatgc tgaaaatgga
     4141 ccagtgttga ttgatgtgcc tattgactat aaagataacg tgacactagg tgaaacaatc
     4201 ttacctgacg agttctacta agacgaggtc taatatgtca gaaacagtta aattatttca
     4261 atacagcacc ttgagttcac tgatggcagg attgtacaaa ggcagtctaa ctatcggaga
     4321 attattaact cacggtgact taggaatcgg tactgttcat atgattgatg gtgaattgat
     4381 tgtattggat gggaaagctt atcaagctat tggtacagat ggtaaagcag agattatcca
     4441 attatcagat gatgtgacag taccctatgc agcggtttta ccacaccata tccaaaaaca
     4501 atttgatatc aatgctgaga cagataataa ggatttggaa gagatgattc taaaaaactt
     4561 tgaaggtcaa aatctcttta agtctttaaa aatcaaagga acgttttcaa gaatgcacgt
     4621 tcgtatgatt ccaaaatctc cacaacataa acgttttgca gatattgcat ctaatcagcc
     4681 agaatttacc agagagaatg tatctggtac gttggttggg atttggacgc cggaattatt
     4741 tcatggtgtg ggggtaaaag ggttccatgt ccactttatt tcagacgatt taactttcgg
     4801 tgggcatgtt atggattact cgctcacaca aggaaaagtt gaaattggaa aggttgacca
     4861 acttgaccaa tgtttcccaa ctcaagatca agaatttttg aaagctaact ttgacctaca
     4921 aaagcttaga gaagatattg atttaagtga ataagaatac ctataaataa ttaataacac
     4981 taaaaataga cagggatatg tcccagtcta tttttttagt ttaaggctat ctattttttc
     5041 ttgtgtggga gtgactcgta aggttttttg accagtgtaa gttacaaaac ctggcttagt
     5101 accacttggt ttattgagtt tttttgcttc aatcatgtct acttgaacaa gattggagag
     5161 acgagcttta gagtaataag cagctaactc ggcggcatct gtcttaacct cgtcacttgg
     5221 attgaggtta tcccgaatta acacatggct tccggggata tccttggcat gaaaccacaa
     5281 ctccccttta cgagccattt taaatgttaa ctcgtcattt tggagattat tacgtcccac
     5341 cataataata gtcttgccat cggatgctaa atattgttca ggttttttac gcttatgttg
     5401 tttatcatgt gcgcgtcgct taataaatcc agtttcaaca agttcttcac gaatatcatt
     5461 gatatcttcc atagaagcat gatttagcga tgtttcaaca gattcaaggt aggtgattgt
     5521 atttttcgta tctgaaataa ttccttttaa atgttttaca gcttctttta atttttgata
     5581 ttttttaaag tagcgttggg cattttggtt gggtgttaaa gctcgatcaa gtgaaatttc
     5641 aatcgtttgg ttggtgtaat aattatcaag aacaacgata tcttgattat ttggcaccat
     5701 agagagatag gtagttagaa gctctccttt ttggcgaaac tcctctgcat tttcagtagc
     5761 tagaagttcg tcttgttgct tagctagttt tttgatattt ttctctaact cactttgaac
     5821 acggtgaatg aggtcactag cttgctgtgc tatgcgatct ttttcagcct tctcttggta
     5881 gtaataatct aacaattgtc caagagactg aaatgtttcg tgactgcttg agaattggac
     5941 agcggaaaac gatttttctg ttaggctagg ataatattct ctcgaaaaga attgacgaaa
     6001 atcgttaagt ttattgtctt tcaaacagtg agataattct agcgcagtat cgcgtcctaa
     6061 accttggaag agctgttgga ggtttttagg gcttaggtca ttggtttgaa gcagctcaaa
     6121 tagagtttga tcagaaatat caaaaggatt gattgctttt gttttgggtg gggcaatgta
     6181 agttgaacca ggaagaatag tacggtagga gttttgcgag aaaccaacat gcttaattga
     6241 ttcaataatt ttatgttcat ttttatcaat caagataatg ttactgtgtt ttcccatgat
     6301 ttcaacaaca agtgtcgctt taatgtgatc accgatttcg tttttattag acacgatgat
     6361 ttctaatata cggtcatttt caatttgttg aatggtttca atcactgcac cttgcaaata
     6421 ctttctcatg atcattgtaa aagtatttgg attttgagga ttttggaaat ttgcttcggt
     6481 agtttggata cgtccaaata cagggtgagc agataggagt agtttatagt tgcgacgatt
     6541 attgcgtatt gttaatacca attcatggtc aaaaggttgg ttaacttttt ggatacgtcc
     6601 tttttcaatc tgttcttgta attcatttgt gaggtgatgt aaaaaaaatc catcaaaaga
     6661 catggcgtcc cccttattct aaaaattata gcttatttat tataccatag gctgagctaa
     6721 aaatattttt ttataacgca aaaggcttgc aattttcaga atatagtata taattataga
     6781 aaaattcatg agaaaggatg actagtaaat gactaaaaca ttatttacca attggcaaaa
     6841 ctggcgtaga taaagttgtg tttatgggtg catagatacg actttttgga gtacttcaaa
     6901 aaagtatcta tgcgctggtc tagtctaaca atagagagcg caaggtaagt tatttatcag
     6961 gcgctttttc ttttgtgcta aactccagaa agtcaggata ttattaaagt gaggagaaaa
     7021 tatgaaaaat aaaggactga tagctacgct aattctattg accatattag tggttgggga
     7081 attattttat aacaaatcag aaaaacatct taatttatca gaaaagcaag ttgttaaaat
     7141 tggtatttta cagtatgtaa cccatgatgc tttggatgct attgaaaagg gagttgaaga
     7201 tggtctagct caagaaggat ataaagggaa aaaagttaaa ttaaccgttt taaacgccga
     7261 agctgatcaa agtaaaattc aagcgatgag taaacaatta gttaatcatc acaatgatat
     7321 tttgattgga attgcgacac catcagcaca aggtttagca gcttcgacta aggatactcc
     7381 tataatcatg ggagctgttt ctgatccttt aggggccaag ttagtaacga atatgaagaa
     7441 accaacaact aatgttacag gattatcaaa cgttgttccg accaagcaaa ctgtgcaatt
     7501 aatcaaggat ataactccca atgttaaacg tataggaatt ctttacgcta gtagcgaaga
     7561 taattctgtg tcacaagtaa ctgagtttac aaaatatgct caaaaagcag ggctagaagt
     7621 tcttaaatat tctgtaccat ctacaaatga aattaaaaca agtatgtcag tcatgaccaa
     7681 aaaagtagac gctgtctttg ttccacaaga taatactatt gcgagtgctt ttagaacagt
     7741 tatcgtagca gcaaaccaag ctaatatacc tgtgtattcc agtgttgata ccatggttga
     7801 acaaggaagt atagcatcag tagcgcagag tcaatatggc ttagggttag aaactgcaaa
     7861 acaggccatt aaagtcctaa gagggaagcc ggttaaagat gtaccagtca aagttattga
     7921 tacagggaag ccaagcctca atttgaaagc agccaaacac ttaggaatta aaattcctaa
     7981 gaagattatg aaacaggcag aaattaccgt taaggttgac gattagaaag agggttttat
     8041 gattatttca tcagtttcac aaggtttatt atgggggata ttaggattag ggatatactt
     8101 aacctttaga atcttaaaat ttccagacat gacaacagag ggctccttcc ctctaggggg
     8161 agcggtttgt gttaccttaa tgaatcaagg ggttaatcct attttggcga cgattctagg
     8221 catgctatca gggatgttag cgggatttgt tactggttta ctttatacta aaggaaagat
     8281 accgacgatt ttagcaggga ttttagtaat gacctcatgc cactctatta tgctaatggt
     8341 tatgaaaaga gcaaatctag gtttaaatga gattcaaaca ttgaaagatt ttcttccgtt
     8401 ttcaaatgat ttaaatcttc tcgtattagg tttgatagct attctcttgg cgatttctgt
     8461 cttaatttat tttctctata cacgcttggg gcaagcttat attgcaacag gagacaaccc
     8521 tgatatggca aagagcttcg gcattgatac cgacaaaatg gaaatgcttg gcttaattgt
     8581 atcaaatggc ttaattgctt tatcaggagc tttagtaagt caacaagatg gatatgctga
     8641 tgtatcaaaa ggtataggtg ttattgttat tgggttagct agtattatta ttggagaggt
     8701 actttactct actggtttaa ctctctttga acgtttaatt gctatagtgg tgggttctat
     8761 tctttatcaa tttttaataa cggcagtaat cgccttagga tttaatacga attacctaaa
     8821 attatttagt gcaatagtgc ttggaatttg tttaatggtt cctgtcctaa aaacgaagat
     8881 tttgaaagga gtacgcttat aatgatgatg aaaatcatag agttaaaaga agcaaccgta
     8941 caagtcagta atggtttagc agaaatgaaa acgatattag atcatgttaa tttgagtatt
     9001 tatgaacatg acttcattac aatattgggt ggaaatggag ctggaaaatc aacgcttttt
     9061 aatgtaattg caggtacctt gatgttaagc agtggaaata tttacatcat gggacaagat
     9121 gttactaatt tatcagcaga aaaacgagct aaatatttat cacgggtatt tcaagatccg
     9181 aaaatgggaa cagcacctag gatgacagtt gctgaaaatt tattagttgc taaatttcga
     9241 ggtgaaaaga gaccgttagt tcctagaaag attactaact ataccgaaga atttcaaaaa
     9301 ttgattgcta gaactggtaa tggacttgat cgccatttag agacacctac aggtttatta
     9361 tcaggtggac aaagacaagc gcttagttta ttaatggcaa ctttgaaaaa accaaatctg
     9421 ttattattgg acgaacacac agcagcgctt gatcctagaa caagtgtttc gcttatgggc
     9481 ttgactgatg agtttatcaa acaagattcg ttgactgcct taatgattac acatcatatg
     9541 gaagatgctc tcaaatatgg aaatcgtgta ttagtaatga aagatggcaa gattgtcaga
     9601 gatttaaatc aggcacaaaa aaacaagatg gctatagctg actattatca attatttgat
     9661 taaaaagaag tgaaatggaa gtgtttcact tctttttcta tacactttgc aagaaattat
     9721 tttcaaaata tgatatacta ataatgctgt aaatcgactt gtagagacaa ggaccgtaag
     9781 tacaaatgga atgaaaataa ggacacactc tttaattcat attcctatta ttgattaata
     9841 aggtttttct tcaaacccaa gaaaagttac cgataaatca attactaatg ataataattg
     9901 gacaatttct tgtctgcttt ataaaaaaga attaacaaaa tttaaggaga aaactcatta
     9961 tgagcgacat aaaaatcatt gctcttggag gtgttcgtga aaacggaaaa aacctatatg
    10021 tagtagaagt caatgattct atctttgttt tggatgctgg actcaaatat cctgaaaatg
    10081 aacaactggg tgttgatgtt gttattccaa atcttgatta cttaattgaa aataaaaaac
    10141 gtgttcaggg gattttctta acccatggac atgcggatgc tattggtgca ttgccttata
    10201 ttatcgccga agtcaaagca cctgtgtttg gttcacctct cacaattgaa ttagctaagt
    10261 tattcgttaa aaatagtaca gcagtcaaga aattcaataa tttccatgtg attgatagtg
    10321 aaacggaaat tgaatttcaa gatgcggtga tttcgttctt taaaacgact cattcaattc
    10381 cagagtcaat gggaattgtt attgggacaa aagagggaaa tatcgtttac acaggtgact
    10441 ttaaatttga tcaagctgca aggaaatatt accaaacaga tctggcgcgt ttagctgaaa
    10501 ttggtcgtga tggtgtttta gcacttttat cagattctgc aaatgctaca agcaatgagc
    10561 aagttgctag cgaatatgag gtcggtgatg aaattaagag cgttattgaa gacgcagaag
    10621 gtagagtcat cgtagcggca gttgcaagta atctcattcg tattcaacaa gtctttgatg
    10681 cagcggcaga aaacggtcgt cgagtagttt taacgggttt tgatattgaa aatattgttc
    10741 gtacggccat tcgtatgaaa cgcattcata ttgctgatga aaatatgatt atcaaaccta
    10801 aagatatgac acgttacgaa gataacgaat tattaatcct tgaaactgga cgtatgggag
    10861 agccgataaa tggtctccaa aaaatggcga tcggacgtca tcgttatgtt caaattaaag
    10921 atggtgattt agtatttatc gtaacaacac caagtattgc aaaagaggca gttgtagcgc
    10981 gtgttgaaaa tctgatttat aaagctggtg gctcagttaa attgataaca cagaatttgc
    11041 gcgtatcagg tcatgcaaat ggtcgcgaat tacaattatt aatgaatcta ttgaaaccta
    11101 aatacctttt cccaattcaa ggagagtata gggatttgtc agcacatgcg ggcttagctc
    11161 aagaggtagg aatgtcagca gatgatattt atattgtgaa gcgtggtgac atcatggttc
    11221 ttgaaaaaga tggcttcttc catagtggat ctgttcctgc tggagatgtt atgattgatg
    11281 gaaatgctat tggtgacgtt ggtaatattg tacttcgtga ccgcaaggta ttatctgaag
    11341 atggaatttt tattgttgtt ataactgtca gcaaaaaaga aaagaaaatt atttctaaag
    11401 ctcgtgttaa tacgcgtggt tttgtgtatg tgaaaaaaag cagagatatt ttgcgtgaga
    11461 gcgcagaatt agttaataca acggtagaag attacttaag taaagataca tttgactggg
    11521 gtgaacttaa agggaaagtg cgtgatgagg tcagcaagtt cttatttgac caaacgaaac
    11581 gtcgcccagc aatccttcct gtggttatgg aagttcgttg atagtgttaa gaggttacca
    11641 aattttggta acctttcttg ctagaatgga ggtttttatg gcatttttta atattgaata
    11701 tcactctaag gtactaggaa cggaacgtca agtcaatgtt atttacccag atgcttttga
    11761 gatgtctgat gataaaattg atgattgtga cattcctgtt ttgtatcttc tccatggaat
    11821 ggggggaaat gagaattctt ggcaaaaacg cacaaatata gagcgtcttt tacgtcatac
    11881 taatcttatc gtcgtgatgc catcaacaga cttagcatgg tataccaaca caaagtatgg
    11941 gttggattat tttgatgcaa tagctattga gttaccaaaa gttttgaaac gtttctttcc
    12001 taatatgtct gataagagag agaaaaattt cattgccgga ttatctatgg ggggatatgg
    12061 agcatataaa atagctctac tgactaatcg tttttctcat gctgcctctt tgtcaggtgc
    12121 actttctttt gactttgatt tattattcaa taatggcaac gataacacta actattggtc
    12181 gggaattttt ggtgatttga ataacactga taacatagag cgtcattcac taaggcgtta
    12241 cgttgagtct tttgatatga agactaaatt ttatgcatgg tgtggttatg aagattttct
    12301 ttttgaagct aatgaagtcg caattgatga acttcgtcaa ttaggtttaa ctattgacta
    12361 ttccaatgat catggtaaac atgaatggta ttactggaat caacaattag aaaaagtctt
    12421 agaatggctc cctgttgatt atgtcaaaga agagcgcctt tcttagtatt agctaaatcg
    12481 ttgaaatcca agtaaagtct tgttatacta aatcattaag ttttgggaaa ttagacacag
    12541 aaggtgagat aaaaaatgag aacactattt agaatgatat ttgctattcc aaagtttatc
    12601 tttagattga tttggaatat catttgggga atattcaaga cagttcttgt tattgcgatt
    12661 attttatttg gcttgtatta ctatgcgaat cacagtcaat cagaatttgc taatcaactt
    12721 agtgacatta ttcagacagg aaaaacattt ttaaattttg ctgacactaa tcagctcaaa
    12781 aattcctttg ctaatctagc tacggataat gttcatcatt cagaaaatac tagatgggat
    12841 aaaaatcagg caactatcta tatagcaact agagattctg agttggcctc tgcttacaag
    12901 caagcaatct ccaattggaa tgcaacaggt gcttttgtgt tacaaacaac agataatcct
    12961 aatgctgata ttattgctaa ggattactca gatgctaaaa cacaagctgc aggtgtagct
    13021 gaaaccgaga gaaatgcttt aactaatcgt atttcaaagg taacagttaa attaaacaca
    13081 ttttacctta agaataaaca gtttggttat gaccatacta ggattgttaa tacggccgaa
    13141 catgaattag gacatgcttt aggtctagga cataatgatc aacaacattc cgttatgcag
    13201 tcaaaaggtt cgcattatgg cattcaagaa gttgatattc aaacgctaaa aaacttatat
    13261 gcataaaaag atgagctaat aaactcatct tttttgtttg aacacttatt ttaggtgata
    13321 taagtgcgtt agcaatagat tattaaaaat aaagaatttg actatcacta ctaactaaaa
    13381 tgcataaaga acaaagaaaa tgttttcaaa aatcacttaa aaaatggtat aatgtgtctt
    13441 gagatagtgt tttgaataga cttatcaaaa atattagata acattaactt aaaaggggaa
    13501 aatatggacg aactaaaaaa acttgctgga gtgaccgctg caaaatacgt taaaaacggt
    13561 atgattgtgg gtttagggac aggttctaca gcatattttt ttgttgaaga aattggacgc
    13621 cgtgtcaaag aagagggatt gcaagtcata ggcgttacaa cgtcaaaccg tacgactgag
    13681 caggcaagag gattaggtat tccgttgaaa tcagcagatg atattgatgt cattgatgtc
    13741 actgtggatg gagcagatga agtagaccct gattttaatg gtatcaaggg gggcggtggt
    13801 gcgctgctca tggaaaaaat agtagctact ccaactaaag aatatatctg ggttgtcgat
    13861 gagagcaaac ttgttgaaac gttaggtgca tttaaattgc ctgttgaggt tgttagatat
    13921 ggttctgagc gtctttttcg ggtttttaaa tcaaaaggtt attgtccttc tttccgtgaa
    13981 acagaaggtg accgctttat cactgatatg gggaactaca tcatcgacct agatttgaaa
    14041 aaaatagagg atccaaagca gttagcgaat gaacttgacc atacagttgg tgttgttgaa
    14101 catggtttat ttaatggtat ggttaataaa gttatagtag caagcaaaaa tggtcttgac
    14161 atacttgaaa aataattatt taaataatcg gttttaggag ctaaccgtaa cctccttaga
    14221 aagaagatat aatgtcacaa tttgatcgca ttcacttagt tgtcttagat tcagtgggta
    14281 ttggagcggc accagatgcc aatgattttg tcaatgcagg agttcctgat ggtgcttcag
    14341 acacgcttgg ccacatttca aaaacagttg gacttgcggt gccaaacatg gctaaaatcg
    14401 gacttggtaa tattcctcgt ccccaagcgc ttaagaccgt ccctgcagaa gaaaatccaa
    14461 gtggctacgc gacgaagtta caagaagtat cacttggtaa ggatacaatg acagggcatt
    14521 gggagatcat gggcttaaat attacagagc catttgatac tttctggaat ggttttccag
    14581 aagatattat cacaaagatt gaggattttt caggacgtaa agttattcgt gaagcgaata
    14641 aaccttattc agggacagct gttattgatg acttcggacc acgccaaatg gaaactggtg
    14701 agctcatcat ctatacctca gctgatccag tcctacaaat tgcagctcat gaggatatca
    14761 tccctctaga agaactctac cgtatttgtg aatatgctcg tagtatcaca atggagcgtc
    14821 cagctctcct tggtcgtatt atcgctcgtc catatgtggg tgaaccaggc aactttacgc
    14881 gtacagccaa ccgtcacgac tatgcagtat caccatttga agataccgta ttaaataaac
    14941 tagatcaagc tggcatcgat acttatgctg ttggtaaaat caatgatatt ttcaatggtt
    15001 caggcattaa tcatgatatg ggacataaca aatcaaatag tcatggtatt gatactttga
    15061 taaaaacgat ggggttatca gagtttgaaa aaggcttctc ctttactaat ttggttgact
    15121 ttgatgctct ctatggtcac cgtcgtgatc ctcatggtta tcgtgattgt cttcatgagt
    15181 ttgatgagcg tcttccagag atcgtttcag cgatgcgtga caaagactta cttttgatca
    15241 ctgccgatca tggtaatgac ccaacttatg caggtacaga ccatacaaga gaatacattc
    15301 ctctcttagc ctatagccca tctttcacag gaaacggcct tatcccagta gggcatttcg
    15361 cagatattt
//
LOCUS       DATRQA010000039        15171 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.039, whole genome shotgun sequence.
ACCESSION   DATRQA010000039 DATRQA010000000
VERSION     DATRQA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15171
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.039"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            163..716
                     /locus_tag="VBD27_000885"
                     /pseudo
     CDS             163..716
                     /locus_tag="VBD27_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
     gene            936..1355
                     /locus_tag="VBD27_000886"
     CDS             936..1355
                     /locus_tag="VBD27_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HEO7024914.1"
                     /translation="MKMTVYFDGNFWLGLIEYDDDGDYKVFRYFFGKEPKDDDVFNFI
                     NHKLNDLIKKYEFVKTDISLKRTNEHKKSPKRMQREINREKRKPVVSTKAQLAMKTIH
                     MSIKNERQLSQKCKKNELRKHRYQLKQEKRYQKKKGH"
     gene            1555..2034
                     /locus_tag="VBD27_000887"
     CDS             1555..2034
                     /locus_tag="VBD27_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7024915.1"
                     /translation="MRDFEELFDKVKPIVMKLRRNYFVQLWEYDDWIQEGRIVLFRLL
                     EEHPYLLDNESKLFIYFKTKFSNYLNDVLRHQDCQKRQFNKMPYEEISEVSHYVKSKG
                     LVLDDYIAYRDTLTKVEETLSDIDKEKFEKLINGERFAGKKKFIRNIQPFFNAFKAD"
     gene            complement(2213..3310)
                     /locus_tag="VBD27_000888"
     CDS             complement(2213..3310)
                     /locus_tag="VBD27_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7024916.1"
                     /translation="MIEKYTKKDGTTAYRLRAYLGVDPMTGKQVRTTRQGFKTEREAK
                     RAEVKLIDDFQRQGAWKSNDKTTFDDVAKLWFEQYRNTVKPSTFLVNQNYYKTILKPH
                     LGQLQMTKITVMICQKFVNCLSRYSGYRLYLSLANRIFKFAVNLGIIDNNPMSKTLRS
                     KCTYKNMDTLTKKYYTKEELNAFLRIVEAEETLEMRLIYRLLSYGGFRIGELIALKDT
                     DFDFRNNTISITKTIAYTKEGWAVQSPKTKKSNRTISMDAETMTLAKLYIKQSIKPLH
                     GSFKLFNFASDTVRKRLDRFILKHGLKRITPHGFRHTHASLLFEAGIPAKIAQERLGH
                     AKIAITMDLYTHLSKKSKDNVADKLAELVAI"
     gene            complement(3483..4097)
                     /locus_tag="VBD27_000889"
     CDS             complement(3483..4097)
                     /locus_tag="VBD27_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024917.1"
                     /translation="MKKPFYKKWYFWTHAILLLVLGFSFFVIYRLAETNIVNEKKIAK
                     LEKTQENKTTSGIRKTISDFTSRFDEELSVRAIKFYLNKDQVVSSFGDEVKLGGGYLT
                     INKPNNDKTRMLATTTDFKNKIIVPIEFKNTTGETKGFDTRDIFAYNGDETISFDSVI
                     SENLDNDGYSVVVKDGETAAASIVFGTNSKIKDIKVRYNSGLWK"
     gene            complement(4229..5008)
                     /locus_tag="VBD27_000890"
     CDS             complement(4229..5008)
                     /locus_tag="VBD27_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7024918.1"
                     /translation="MSSKKLENKEIFSKNLAFYMERKRVDRNTLCADLGLKYTTVRDW
                     LKGITYPRIGKIELLAEYFSINKSDLIEEHSTNGATSKVNFDPRQAILLSNYSKLNNV
                     RKNKLLAISETLLSEEQGKVINLQEKMAEYGSRKRVSLSVPGKVSAGTGYWQEDDYDT
                     EVDFYADEIPDEKNYDTVAVVVGHSMEPKIKNGDFLFIKLADQVDINKIGIFQVNGEN
                     YVKKLKNDYLQSLNPKYADITPAEGDDFRTIGEVVDIYREG"
     gene            complement(5507..5719)
                     /locus_tag="VBD27_000891"
     CDS             complement(5507..5719)
                     /locus_tag="VBD27_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024919.1"
                     /translation="MKISNSKDLALAIVASSSPTLSIEDKIKLYEDSLEAIKQHNLPF
                     IEAEKQEQINNGKVIAEALERGESLF"
     gene            5778..5936
                     /locus_tag="VBD27_000892"
     CDS             5778..5936
                     /locus_tag="VBD27_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024920.1"
                     /translation="MNWKKLMFGDLEHTFTSRNGKEKTSIEFEGGLLPALLVLGGITW
                     LIAWFITK"
     gene            complement(6034..6675)
                     /locus_tag="VBD27_000893"
     CDS             complement(6034..6675)
                     /locus_tag="VBD27_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024921.1"
                     /translation="MNFFNFLLCVFKFTSEYLIKNWIALIALFLSYSNYRRNNLQVEL
                     IAAPVSDWILSVILDNGESIYNPNGTLRANIKIINPSNVDVSYFDLIVFDKNRKYQHY
                     YQKQNNIINDLTGREAIAAVQPDGNTILIEVPEADCGVLKAHSMTRMDLIIQTSEITD
                     RLFVAFKVAKKKKLFKANKAGYVNSPYQSFSASFPVELSKKPHYEDILKDLHE"
     gene            6733..6936
                     /locus_tag="VBD27_000894"
     CDS             6733..6936
                     /locus_tag="VBD27_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024922.1"
                     /translation="MEEILSSIAKSLESIATEFKAQNSYREEMKQNMEQMEKIILDIQ
                     SDPFGLKHLKDKALSEKASKQKG"
     gene            complement(6920..7279)
                     /locus_tag="VBD27_000895"
     CDS             complement(6920..7279)
                     /locus_tag="VBD27_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024923.1"
                     /translation="MDKLTNDAKFLLSSMFVKYNERRKDKISKEKSRNFEDIQFIKEN
                     IMNEWSEEDVLDTCFELRKHGYISATAASDTLYLISLTTEAIAELEKQDQAKTLSGRI
                     EYWLEFAKKIKDAIPFA"
     gene            7352..7558
                     /locus_tag="VBD27_000896"
     CDS             7352..7558
                     /locus_tag="VBD27_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7024924.1"
                     /translation="MTKMTLKALRATKNWSQEEAARALKVSKDTWGNWERGNTEPSVT
                     KAYQIAYIFDISLDDIIFLPNIAV"
     gene            7612..8571
                     /locus_tag="VBD27_000897"
     CDS             7612..8571
                     /locus_tag="VBD27_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024925.1"
                     /translation="MQEITTQNDFDYSLVDFETQEFLQERSNIIYGIQSKSAYEIGKQ
                     LVKAQEVLAKNRYGCFEEWYSSLGFRKTKAYEYINHYNFIRSQSEQLNIETFEELPKK
                     LQSEMSKPSANPELNQKVFDGDITTHKQYKELERQLKLAEADKERLKQQNERLAEQAL
                     SAKVVEKEVVIEKVPDDYESTKQLNRTLLDKNKELSTTLEEAEWELDSKKLELSTIKL
                     ESQRAIEVTNQIRHLEGKKEKLENLVTSISELSSIISDVQNFFDTKMAPLRFKPIINN
                     VNAHYSVTEVTKMVNTVQTWCDEMYKIIPSGNRKTVEEVIINE"
     gene            8564..9085
                     /locus_tag="VBD27_000898"
     CDS             8564..9085
                     /locus_tag="VBD27_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024926.1"
                     /translation="MSKELSEMNEKDALEVVKGMTIEDMMIEVLSSQKQVKASQEAIK
                     KDVEEVCSLAIEIDKKVHIDDVEASEIKSIISRQSYEFAKEYFDNRKVVPSANLFASK
                     KGQFIRVQHSRLKHHFNVTKYTHIKHTEAEKAISYLKSLSYGLFTPFETRETPKQKEI
                     IELENKNNGDDVA"
     gene            9104..9295
                     /locus_tag="VBD27_000899"
     CDS             9104..9295
                     /locus_tag="VBD27_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024927.1"
                     /translation="MNEKITDEQLNKYLEIRTELGEENFKFINACYDEVLHLKKEEAT
                     KNIKFEITDFSKFRDFAKK"
     gene            complement(9323..9499)
                     /locus_tag="VBD27_000900"
     CDS             complement(9323..9499)
                     /locus_tag="VBD27_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024928.1"
                     /translation="MISSQRIKETLDRHDVSESDKLSVALAEILNKHLSGKNLSETVH
                     EHDKRMARMRGEIM"
     gene            9614..9790
                     /locus_tag="VBD27_000901"
     CDS             9614..9790
                     /locus_tag="VBD27_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7024929.1"
                     /translation="MKKKLNKILIDKGMSKKELSEKTGISYNTIMNIGKRDISFNKMK
                     KIADVLDISLNEFR"
     gene            9869..10126
                     /locus_tag="VBD27_000902"
     CDS             9869..10126
                     /locus_tag="VBD27_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024930.1"
                     /translation="MQSQLTYDLLKKQIAEELFDEFKELIQEKDLANRWVNQTALVNE
                     YGYSWQTIKRMESYGLKSFKNGKDKMYCLADVNEIKHLMKQ"
     gene            10156..10299
                     /locus_tag="VBD27_000903"
     CDS             10156..10299
                     /locus_tag="VBD27_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024931.1"
                     /translation="MKLFNFIFAKPKKQEEKPKWTIETHGWEANARRYDNMIEHNNRG
                     RTC"
     gene            10375..10575
                     /locus_tag="VBD27_000904"
     CDS             10375..10575
                     /locus_tag="VBD27_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024932.1"
                     /translation="MNNDWKKRYINNQIDFFNDIKTCVKERWPNMKGNDVDKISIAIY
                     RNWPVSDINSTLEVIETDLEKL"
     gene            10572..10862
                     /locus_tag="VBD27_000905"
     CDS             10572..10862
                     /locus_tag="VBD27_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024933.1"
                     /translation="MKCYVNKQKKLAIDMNYKDKFGKFSSDSIQILEGKLTDSIQIDV
                     ENAMKEIIDKYSQLFDTPIIDDLFTEKEKQLKQSYDVETTLTEMFEVEYEDN"
     gene            10849..11532
                     /locus_tag="VBD27_000906"
     CDS             10849..11532
                     /locus_tag="VBD27_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7024934.1"
                     /translation="MKITKATEITKTHNWRILIYGKPGNGKTYLINYLKGKTLILDMD
                     HSSKTIAGNENIDIIQFDRTHPSDFMTEFLTELPELIKEYDNLVIDNITSWQSDWFIE
                     QGRKSKNGITNELQQYNMWTNYYLRVLTTIYSQPINIFVTAWESTQDLTLESGQMITQ
                     YVPDIRKQVLSQTLGLTDVVGRIQVNPKTGGHGILLEGSDGLYAKNRLDNRTVCKAEE
                     LFNFEGSDA"
     gene            11568..12971
                     /locus_tag="VBD27_000907"
     CDS             11568..12971
                     /locus_tag="VBD27_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7024935.1"
                     /translation="MNEARRHILKHNVMIVSPPGSGKSVVISDIAKSATQKNGYVLFL
                     VHRKELIDQITNSFKFHGIDMNKVDLMTVGKAKNRLDKLTKPTLIITDEGHHGKASTY
                     QMIYEYFSDVPRIGFTATPWRLSGDGFTDTYDVMVLGKTVEWLINNNKLAPYDYYSVL
                     SIDTAKLKVQNGDYSNKSIDESFGKKIFGDVVQEYIKKANGQKAILYAHSVEASQAFA
                     KEFQSMGINAIHADAKTPKAKRDKSMKDFRDGKIQVICNVDLISEGFDVPDCTVTILC
                     RPTKSLVLFLQQSMRSMRYQPNKKAIILDHVGNWNIHGLPDTPHHWENYFRGGWKKKS
                     NKTNTVHAKECPVCSALWPLSQQLCELCNHDFGLKEKQEKERIEAELELIKRERFRIK
                     QLANKKFGKDLKTNWEIAQARVKDAGKGKPLYKLIYFYLKTDWVETNVNELAEVTGKS
                     EKEIYSAYNWLKKKLRG"
     gene            12976..13458
                     /locus_tag="VBD27_000908"
     CDS             12976..13458
                     /locus_tag="VBD27_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEO7024936.1"
                     /translation="MAGFTTDFSEVKEHAEFKEQPYEMIVYDAYEAVNDRNGKKRVVI
                     DYVVRNDIKQEMQNYHLWDEQYPNSQTGKYHIGILMGKAKALGIKEGQHYDSFEAFLN
                     DFKGRTAKVTVKLDEYNGNKYPKVRYANQSDVPESHHIWKEKTTGFTQAEIEEDDLPF
                     "
     gene            13476..15029
                     /locus_tag="VBD27_000909"
     CDS             13476..15029
                     /locus_tag="VBD27_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HEO7024937.1"
                     /translation="MTSNEFIEALSKLTTETDWGDPIFGESVLKAELRKHLFKIVPID
                     HNGYIHKLFYSEMVKDEDVMYFVSDGRKTYRFLFGDTILKTDKQGNEYLTYSVENNFP
                     PFAKLVIDYILGTYTFFENKLYDIRYKQFKLIDDFTLQTKYGFKDSGHILEILQGIHK
                     TLNIQPINYIEPYQIACKDFIIDLENSEIINQPPLQNVSYFKYYEVDYKTAINSKSIA
                     EEYLEYVIADSNSLNNAILQSYFIAQVACGVRPKTNFFISKSGVRTGKGLRHIALSGL
                     FNKIDVELDTLKSNGFEALQAWAMFSGGEMALATEQGDIQGNAMERVLKIIATEKTHV
                     ARAIGQNQSIVNLTSVLCIDTNRTVALSDEMNGRKVLIQFKDRPKTETDYERESVFRK
                     YWLAFTDRDKNPKIDGCIGFLLNSLERFQKIGKWYQWKDVEVFNDIDLDEFQVALINA
                     LQEVDFVQRTDNKEVIDLSLQVYGKSNHAVSKAISEIGVRSRSKKVNGKTVRGYEIEN
                     KTRFDKYIL"
BASE COUNT         5400 a         2456 c         2723 g         4592 t
ORIGIN      
        1 cttaaattga cacttggagc attcaatcac gtgattgtaa taactaaaaa ttgtttaaaa
       61 tttaaggatg tatttcttgt tgatcttact atctgatcta ataatggtat ataataagat
      121 taaaatttaa acattgtctg gagatgtagc aaaggagata aaatgactaa aatttatcat
      181 atgattttat gtaattctga tataatgaag attttagcta taataaaatc tcttccactg
      241 aatgattgtt ggctgtgtgc tggcacattg agaaacttta tatggaataa actttctgga
      301 ataaatgaga cattgacatc agacattgat gttgtatttt ttgataaaaa tatttcttat
      361 gaagaaacgg tggtattaga acaacaatta aaggataact atccacagta cgattgggaa
      421 cttaaaaatg aattttacat gaacactcat tctcctaata ccccaaagta tactagctct
      481 aaagatgcta tttcgaaatt tcctgaaaag tgtactgcag tgggagaaag gttagatgat
      541 agaaatcaac tcgaacttta cctccctctt atggtgaaga ggaaatatta aatttcattg
      601 ttagtcctac gccatatttt gaagaggatc tattacgtta taatgtttat ttgaagcgag
      661 tggataagaa gaaatggaat aatatttggc ctcgtttaac aatcataaaa aaatgataac
      721 gttactggag ttggcttaac agcttttaaa tttttagact gacaaatagt atagaccttt
      781 aataaggtcg tttatcgata atcatagtgt actgtgttat aatcgttata gcataaaaac
      841 acgttgcagt tggtagtctg caagcgttca gtgaacgtca gtaaccttcc ctccaggtcg
      901 tccattgcaa tttaatattt aaaggagggg gctgtatgaa aatgacagtc tattttgatg
      961 gtaatttttg gttgggtttg attgagtacg atgatgatgg tgattacaaa gtttttcggt
     1021 atttttttgg aaaagaacct aaagatgatg atgtttttaa ttttataaat cataaactta
     1081 atgatttgat aaaaaaatac gagtttgtta aaacggatat ttctctaaag cgtacgaatg
     1141 aacacaaaaa aagtcctaaa agaatgcaaa gagaaattaa tagagaaaaa cgtaagccag
     1201 tagtatctac aaaagcgcaa ttggcgatga aaactattca tatgagtata aaaaacgaac
     1261 gtcaattaag tcagaaatgt aagaaaaatg agttaagaaa gcatcggtac caattaaaac
     1321 aagaaaaacg ttaccaaaag aaaaaagggc attgattgtg tcatcatata aaaagcttga
     1381 gagtatcatc tcaagctttt tatatgttaa ctaatatgag ttactaaata gtctgataat
     1441 gctcaattaa tgtgttttgg gaaaaaatca tttccttaaa ggacaccatt gtcatgttgt
     1501 tatcacctta ctttttttat acttagtata tgtttaagaa aggaataagt tgttttgaga
     1561 gattttgagg agttgtttga taaagtaaag ccaatcgtaa tgaagctgag aagaaattat
     1621 tttgttcaac tttgggaata tgatgattgg attcaagaag gacgtattgt actttttagg
     1681 ttgcttgaag agcatccgta tttattggat aatgaaagca aactattcat ttactttaaa
     1741 acaaaatttt caaattattt aaacgatgta ttaagacacc aagattgtca aaagcgacaa
     1801 tttaataaaa tgccttatga agaaatcagt gaagttagtc attatgttaa gagtaaagga
     1861 ctagttttag atgactatat tgcttatcgt gatactttaa caaaagtgga agaaactttg
     1921 tcagatattg ataaagaaaa atttgaaaaa ctgattaatg gtgaacgttt tgcggggaag
     1981 aaaaaattca tacgaaatat tcaacctttt ttcaatgctt ttaaagcgga ttaatcgatt
     2041 ttatttctgc agtagatagc tgcagaattt ttttataggg aaagttcgct gatatattcc
     2101 tgtgaaagcc tttgtttttt gttataatat aagattacct ctcagaatca ttgatatcaa
     2161 ggctttttta aagtctaaaa acagacttac tacgttcccc actacgtttg tgttaaatag
     2221 cgacgagttc ggccaatttg tcagcaacat tatcctttga ttttttggat aagtgagtat
     2281 ataaatccat cgtgattgct attttagcgt ggcctaaccg ctcttgtgca atcttagcgg
     2341 gaatcccagc ctcaaacaac aacgaagcgt gggtatgtct aaacccgtgg ggagtaatcc
     2401 tttttaatcc atgcttcaat ataaatctgt ccagtctttt tctcacggtg tcgctagcaa
     2461 aattaaacaa tttaaacgat ccgtgtaaag gtttgatact ttgcttaata tataatttgg
     2521 ctaacgtcat ggtctcagcg tccattgata tggtgcgatt gctttttttg gttttaggag
     2581 attgtacagc ccatccttct tttgtataag caatggtttt tgtaatgctg atagtattgt
     2641 tgcggaaatc aaagtcggta tcttttaaag ctattaattc accaatccta aaaccgccat
     2701 aactcagcaa tctataaatc agacgcatct ctagagtttc ttcagcttcc acaatcctca
     2761 agaaagcatt caattcctct tttgtgtaat attttttggt gagtgtatcc atgtttttgt
     2821 aagtgcactt tgatctcaac gtcttgctca tggggttatt atcaataata cctaagttga
     2881 cagcaaattt aaaaattctg tttgctaaac ttagataaag cctataaccg ctatatcgag
     2941 ataggcaatt cacaaatttt tgacaaatca tgacagttat cttcgtcatt tgcagttgtc
     3001 ctaaatgtgg ctttaaaatt gttttatagt agttttggtt aaccagaaat gttgacggtt
     3061 tgactgtatt tcggtactgc tcaaaccaca gtttggctac atcgtcaaat gtagttttat
     3121 cgttgctttt ccaagcgcct tgacgctgaa aatcatcaat aagttttacc tcggctcttt
     3181 tagcttctct ttctgtttta aacccttgcc tagttgtcct gacttgttta ccagtcatgg
     3241 gatcaacacc aaggtatgct cttaagcgat aggcagttgt gccatctttt ttagtgtatt
     3301 tttcaatcat tgttttttcc tctctctttg cgctggggag tgctgatttt gagataggat
     3361 attggcatca cctcctaaaa atgatataat taggtacaag aaaacgacct ttttaatggt
     3421 tgtttcttat actagattcg ccccacgctc agaccctccc aaatttgagc gtggggcttt
     3481 ttttatttcc ataatcctga gttatatctc actttgatat ctttaatctt gctgtttgtc
     3541 ccaaaaacaa tactagccgc tgctgtttct ccatctttta caacaacgct atatccgtca
     3601 ttatctaaat tttcgctgat aactgaatca aaagaaatag tttcatctcc attgtaggcg
     3661 aaaatatctc ttgtatcaaa accttttgtt tccccagttg tatttttgaa ttctatcggc
     3721 acaatgattt tatttttgaa atctgttgtt gttgctaaca ttcttgtttt gtcattattt
     3781 ggtttattta tagttaagta acctccaccc aatttaactt catcaccaaa agacgataca
     3841 acttgatctt tattaagata aaatttaata gctctgacag acaattcttc gtcaaaacga
     3901 ctggtaaaat cggatatggt tttcctaata cctgacgtcg ttttattttc ttgagttttt
     3961 tctaattttg ctattttctt ctcgttaaca atattagtct ctgccaatct gtaaatgaca
     4021 aaaaacgaaa atcctaaaac taatagtagt atagcgtgtg tccaaaaata ccacttttta
     4081 taaaaaggtt ttttcatatc ataaatctcc taaattaaat ttttaaactc atttactacc
     4141 atcgcttcat cgcagatggt gtttaaccta tacgctttca taaatctata aacattaaaa
     4201 tcttctatgt cgtcaagttt tccacagact acccttctct ataaatatct accacctcac
     4261 caatggttct gaaatcatct ccttcagctg gtgtgatgtc agcatacttt ggattaagtg
     4321 actggaggta gtcgttcttt aatttcttga catagttttc accgttaact tggaaaatac
     4381 caattttgtt aatgtcaact tgatcagcca gcttaataaa taaaaagtcg ccatttttta
     4441 tttttggttc cattgagtga ccgacaacaa ccgcaacggt gtcatagttt ttttcatctg
     4501 gtatttcatc agcgtagaag tcaacctctg tgtcatagtc atcctcttgc caatatccag
     4561 taccagcaga cactttaccg ggtacagata gacttacacg ttttctggag ccgtattctg
     4621 ccatcttctc ttgcaagttt atcactttgc cttgttcctc agataaaagt gtctcagata
     4681 tagccagcaa cttattcttt cgtacgttgt taagttttga atagttagat aagagaattg
     4741 cttgccttgg atcaaagttg actttggaag ttgctccatt agtagagtgt tcttcaatca
     4801 aatctgactt gttaattgaa aagtattcag ccaaaagttc tattttccct attcgtggat
     4861 aagttatacc cttgagccaa tctctaaccg tagtatactt cagtcctaag tctgcacata
     4921 gtgtatttct gtctactctt tttcgctcca tataaaatgc aaggttttta gaaaatatct
     4981 ctttgttctc taattttttt gaagacattt ttgcacctcc ttataatgta tatattacgg
     5041 caaaaacgca aaaaagtaaa taaaaaaata aaaaaaccgt aaaaaaacgc aaaaaaatac
     5101 ttgacattgc ggtttaaccg cagtataata taatcaagct taaggaatta agcaaaacga
     5161 aaggaggtac agctgatgaa gtcgaggcta aacaaaaagc ctaaacacaa agaactagaa
     5221 gtcgaaatca aaattctttg gtttaagctt agaataaaac atctgattac taggtaatcg
     5281 gatagggggt gaaactcccc cacccctaat aggggtaagt ttagtttagc acattggctg
     5341 tatctccgca agaatgaaag gagaagttat gagtgagcaa aatgaaaagt ttatcggagt
     5401 gctaaaagat atgcacatgc aaattttgcg agatttcgta aacgcagaag accccttaac
     5461 aaggtgcgaa cttgctaaag ggttcctcga aattggcgac tatttatcaa aacaatgact
     5521 cgccacgctc aagagcttcg gctataactt taccattatt gatttgttct tgcttttcgg
     5581 cttcaatgaa aggtaaatta tgttgcttaa tagcttctaa agagtcttcg taaagtttga
     5641 ttttatcttc gatagacaaa gtagggctag aagaagcaac aatagcgaga gctaaatctt
     5701 ttgaattaga aattttcatt agcttatcct cctttctgct aggataagtt gattataaca
     5761 tttttaggag gtacaaaatg aattggaaaa aactaatgtt tggcgatcta gaacacacgt
     5821 ttactagtcg taatggcaaa gaaaaaacaa gtattgaatt tgaaggtggc ttattgccag
     5881 ctctgttagt gctaggtggt atcacttggc tgatcgcttg gtttattaca aaataaaaac
     5941 tcccaaaagg agtggaaagg agggaaggca tggaaaaaat taaatacggt gtactaggtt
     6001 cttccaagat atttcacaca agaaaatctg ctctcactca tgcaaatctt ttaggatatc
     6061 ctcgtagtgc ggtttttttg ataattccac agggaatgac gcagaaaatg attgataagg
     6121 tgaattaaca tatcctgctt tattagcttt aaatagtttt ttctttttag ctactttaaa
     6181 agcaacaaag agtctatctg tgatttcaga tgtttgtatg attaaatcca tccttgtcat
     6241 actgtgggct tttaatactc cacaatctgc ctctggaacc tcgataagga ttgtattgcc
     6301 atcaggctgt actgcggcta tagcctctct acctgttaaa tcgttaatta tattattttg
     6361 cttttggtaa taatgctgat attttctgtt tttatcaaaa acaatcaagt cgaagtagct
     6421 tacatcaaca ttagaaggat tgatgatttt aatgttagct cttaatgtac catttggatt
     6481 atatatgctt tcaccgttgt ctaaaataac gctcaaaatc caatctgaaa caggagcagc
     6541 aattaactcg acttgtaagt tatttcttcg gtagtttgaa taagatagaa acagagctat
     6601 taaagctatc caatttttta ttagatattc acttgtaaac ttaaaaacac acaataaaaa
     6661 attaaaaaaa ttcatttcaa cctcacattt ttatttaaat tataccacag aaacagaaag
     6721 ggggtgggga agatggaaga aattttgtca agcatagcaa aaagccttga atccattgcg
     6781 actgaattca aagctcaaaa ttcttacaga gaagaaatga aacaaaatat ggaacaaatg
     6841 gaaaaaataa tattagatat ccaatcagat ccatttggac tcaaacactt aaaagataaa
     6901 gcgttgtccg aaaaagcttc taagcaaaag ggatagcatc ctttattttc ttagcaaact
     6961 caagccaata ttctatcctg ccagataaag tcttggcttg gtcttgtttt tcaagttcag
     7021 cgatagcttc ggttgttaac gaaattagat aaagcgtatc gcttgcagcc gtcgctgaaa
     7081 tatagccatg ttttctaagt tcaaaacaag tatctagtac atcttcttca gaccattcat
     7141 tcataatgtt ttctttgatg aattgaatat cttcgaaatt gcgagacttt tctttagaaa
     7201 ttttatcttt acgtctctcg ttgtatttga caaacattga acttaaaaga aacttggcat
     7261 catttgttaa tttatccata tattcacctc ctttctgctc acattatagc agattagagg
     7321 tactaaaaac agatagaaag ggggtgggag aatgacgaaa atgacgttga aagctttaag
     7381 agcaacaaaa aactggagtc aagaagaggc tgcaagagct ttgaaagttt ccaaagatac
     7441 atggggcaac tgggaaagag gtaatacaga gccaagtgtt acaaaggctt atcaaattgc
     7501 ctatatattc gatatatcat tagatgatat tattttttta ccaaacattg cggtttaaac
     7561 gcaaactctt agaaatagaa caaaactaac acaacactag aaaggagaaa aatgcaggaa
     7621 ataacaacac aaaatgattt tgactattca cttgttgatt ttgaaactca agaattttta
     7681 caagagcgtt caaatattat ctatggaatt caaagcaaaa gcgcttatga aataggtaag
     7741 caacttgtta aagctcaaga agttttggca aaaaatagat atggttgttt tgaagagtgg
     7801 tattcaagcc ttggatttag aaaaacaaaa gcttatgagt acattaatca ttataatttc
     7861 attcgttcgc aaagcgaaca attgaatatc gaaacatttg aagaacttcc aaaaaagctt
     7921 caatcagaaa tgtcaaagcc gtctgccaat ccagaactca atcaaaaagt ctttgatggg
     7981 gatattacaa ctcataagca atacaaagag ttagaacgcc aactcaaact agcagaagct
     8041 gacaaagaac ggttaaaaca acaaaatgag agactagccg aacaagcctt gagtgctaaa
     8101 gtcgttgaaa aagaagtcgt aatcgaaaaa gttccagatg attatgaatc tacaaagcaa
     8161 cttaaccgga ctttactaga taaaaataaa gagctttcca caacacttga agaagctgaa
     8221 tgggagctag atagtaaaaa gcttgagtta tcgacaataa agcttgagtc acaacgagct
     8281 attgaagtaa caaaccagat tcgtcacctc gagggcaaaa aagaaaaact tgagaacctt
     8341 gttacttcga taagtgagct gtcctcaatc attagtgacg tgcaaaactt cttcgatacg
     8401 aaaatggcgc cacttcgttt taagccaatt atcaacaatg tcaatgcaca ttattcagtt
     8461 acggaagtaa ccaagatggt taacacagtc caaacttggt gcgatgaaat gtacaaaatt
     8521 attccatctg gaaatagaaa aacagtagaa gaggtaataa tcaatgagta aagaattatc
     8581 agaaatgaat gaaaaagacg ctcttgaagt tgtcaaagga atgacgattg aagatatgat
     8641 gattgaagtc ttatcttctc aaaaacaagt aaaagcttct caagaagcca ttaagaaaga
     8701 tgttgaagag gtttgttctt tagcaattga aattgataaa aaagtccata ttgatgatgt
     8761 ggaagctagt gaaattaaaa gcattatcag tcgtcaatcc tatgaattcg ccaaagaata
     8821 ttttgataat agaaaagttg ttccaagcgc aaatctattc gctagcaaga aaggacagtt
     8881 tattcgtgta cagcattcac gattaaaaca tcatttcaat gtgacgaagt acactcatat
     8941 caaacataca gaagcagaaa aagctatcag ttatttaaaa tcactatctt atggtttatt
     9001 cacaccattt gaaactcgtg aaacaccaaa acaaaaagag attatcgaac tggaaaataa
     9061 aaataacggt gatgatgtcg cttagaacta gaaaggaaac aacatgaacg aaaaaattac
     9121 agatgaacaa cttaacaaat acttagaaat cagaactgaa cttggggaag aaaactttaa
     9181 atttattaac gcttgctatg atgaagtttt gcacctaaaa aaagaggaag cgacaaaaaa
     9241 tattaagttt gaaattacag atttttcaaa atttcgtgat tttgctaaaa aatagccctc
     9301 agaattaact gagagcttct agttacatga tttctccacg catacgtgcc atacgtttgt
     9361 cgtgttcatg cacagtttca gagagatttt tgccagataa atgtttattg agtatctctg
     9421 ccaaagctac tgataattta tctgattcag acacgtcgtg tctatccaac gtctctttaa
     9481 ttctttgtga actaatcata ccttatcctc cttcctatag gatagctaca ttatataaca
     9541 gaaaggtaca aaacacaata tgttgtgtat cttagacgat taaaaacaca atatgttgat
     9601 ttttaaaagt attatgaaga aaaaattgaa caaaattctc attgataaag gaatgagtaa
     9661 aaaagagtta tcggagaaaa cagggattag ctacaacacc attatgaata tcggtaaaag
     9721 agatatttcg tttaacaaaa tgaaaaaaat cgccgatgtc ttagacatta gcctaaacga
     9781 attcagataa aacaaaaaaa gccactgcgg gaacagtgac taaccaaaat aatttaacta
     9841 attataccac gaaagaggtg ataaatccat gcaatcgcaa cttacttacg atctactcaa
     9901 aaagcagata gcagaagaac tttttgatga gtttaaggag ctcatacaag aaaaagattt
     9961 agcgaatcgg tgggtcaacc aaacagcact cgtcaatgaa tacggctact catggcaaac
    10021 tataaaacgc atggagagct atggccttaa atcttttaaa aacggtaaag acaaaatgta
    10081 ctgtcttgct gatgttaacg aaattaaaca cttaatgaaa caataagcgc tggggagtgc
    10141 tagaggagaa acaacatgaa actatttaat tttatttttg caaaaccaaa aaaacaggaa
    10201 gaaaaaccaa aatggacgat tgaaacacat ggctgggaag ctaatgcacg acgttacgac
    10261 aatatgattg aacataataa tagagggcgt acatgttaaa agaagtatta ctagaaattg
    10321 aattcttacg tgatgaaaat agacgactaa ctaatgagtt gctggaggta ttaaatgaat
    10381 aacgattgga aaaaaagata cattaacaat caaatagact ttttcaatga catcaagaca
    10441 tgtgtcaaag aaagatggcc aaatatgaag gggaatgatg tcgataaaat ttcgatagcg
    10501 atttacagaa attggcccgt aagtgacatc aatagcacac tagaagtgat agaaacggat
    10561 ttggaaaaat tatgaaatgt tatgtcaata aacaaaaaaa actagcaata gacatgaatt
    10621 ataaagataa attcggaaaa tttagttctg actctattca gattttagaa ggtaaattaa
    10681 ctgattctat tcaaattgat gtcgaaaatg ctatgaaaga aattatcgat aagtatagtc
    10741 aattgtttga tacgcctata atcgatgatt tattcacaga aaaagaaaaa caattaaagc
    10801 aatcttatga tgtcgaaact acattgacag aaatgttcga ggtggaatat gaagataact
    10861 aaagcaacag agattacaaa gactcataat tggcgcatat taatctatgg caaacccgga
    10921 aacgggaaaa cttacttaat taattattta aaaggaaaga cactgatttt agacatggac
    10981 cattcatcta aaacgattgc cggaaacgaa aacattgata ttatccaatt tgacaggacg
    11041 caccctagcg actttatgac tgaattcttg acagaattac cagaacttat caaagaatat
    11101 gacaatttag tcattgataa tatcacaagt tggcaatcag actggtttat cgaacaaggt
    11161 cgcaagtcta aaaatggaat cacaaacgaa ttgcaacaat acaacatgtg gaccaattac
    11221 tatttacgag tattgactac aatttacagc caacctatta atatttttgt gactgcttgg
    11281 gaatcaacgc aagatttaac gctcgagagc ggacaaatga ttacacaata cgtcccagat
    11341 atccgcaaac aagtcctaag ccaaacgcta ggtttaaccg atgttgtcgg acgtattcaa
    11401 gtaaatccaa aaactggtgg acacgggatc cttttggaag gaagcgatgg actgtacgca
    11461 aaaaataggc ttgataatag gaccgtttgt aaggcggaag agttgtttaa tttcgaaggg
    11521 agtgatgcgt aacggtatac catttacacg aatatcaaac agaacttata aatgaggcaa
    11581 gaagacatat tttaaagcat aatgtgatga ttgttagtcc acctggaagt ggtaagtcag
    11641 tagtcatttc tgatattgct aagtcagcga ctcaaaaaaa cggatacgtt ttattcttgg
    11701 tccatcgcaa agaattaatt gaccaaatca ctaatagttt taaattccat ggaattgaca
    11761 tgaataaagt ggatttaatg acagttggta aagctaaaaa ccgcttggat aaattaacaa
    11821 aaccaacctt aattatcaca gatgagggtc atcatgggaa agctagtacc taccaaatga
    11881 tctatgaata cttttcagac gtgccgagga ttggttttac tgctacacct tggcgtctat
    11941 caggcgacgg ttttacagac acttacgatg ttatggttct tggtaaaacg gtcgagtggc
    12001 ttatcaataa taataaactt gcaccatatg attattatag tgttctatca attgatactg
    12061 cgaaattaaa agtacaaaac ggagattact ccaataaatc gattgacgaa tcatttggta
    12121 aaaagatttt tggtgatgta gttcaagagt atataaaaaa agcgaacggt cagaaagcta
    12181 ttttatacgc tcactcggta gaagcatcgc aggcatttgc taaagaattc caatctatgg
    12241 gaattaatgc aatacacgca gatgcaaaaa cgcctaaagc caaacgggat aaaagcatga
    12301 aagatttccg tgacggtaaa atacaagtta tctgtaacgt tgatttgata tcagaaggtt
    12361 tcgatgtccc agattgtaca gtaactattc tttgtagacc tacaaaatca ttagtattat
    12421 ttttacaaca gtctatgcgg tcgatgagat atcaacctaa taaaaaagct atcattttag
    12481 atcacgtagg aaactggaat attcacggct tacctgacac accgcatcat tgggagaatt
    12541 atttccgagg agggtggaag aagaagtcga ataaaactaa cacggtacac gcaaaagaat
    12601 gtcctgtgtg ttcagctttg tggccactta gtcaacagct ctgtgaatta tgcaatcatg
    12661 attttggatt gaaagaaaaa caagagaaag aacgcataga ggcagaactt gaactcataa
    12721 aacgtgagcg gtttagaatc aaacaacttg ctaataagaa gtttggtaaa gatttaaaaa
    12781 caaactggga aattgcccaa gctagagtta aagacgctgg caaaggaaaa ccattatata
    12841 aacttatcta tttctactta aaaactgatt gggtagaaac aaacgttaat gaacttgccg
    12901 aagtaacagg caagtcagaa aaagaaatat atagcgccta caattggcta aaaaagaaat
    12961 taagaggata aaaacatggc aggatttaca acagattttt cagaagttaa agaacacgca
    13021 gaattcaaag aacaaccata cgaaatgatt gtttatgatg catatgaagc agtaaatgac
    13081 cgtaacggca aaaaacgtgt tgttattgac tatgttgttc gaaacgatat caagcaagaa
    13141 atgcaaaact accacttatg ggatgagcaa tatcccaact cacaaactgg gaagtatcat
    13201 atcggcattt taatgggtaa agcaaaagca cttggtatca aagaggggca acactacgat
    13261 agttttgaag cgtttttaaa cgacttcaaa ggacgcactg caaaagtaac cgttaaactt
    13321 gacgaatata acggaaataa atacccgaaa gttcgttacg caaatcaaag cgatgtgcct
    13381 gaaagccacc acatatggaa agaaaaaaca actggattta cacaagctga gattgaagaa
    13441 gacgatctac cgttctaatt tagactgggg gataaatgac atcaaatgaa tttattgaag
    13501 ctttatcaaa attgaccacc gaaaccgatt ggggagaccc aatcttcggc gagtcagtgc
    13561 ttaaagctga attacgaaaa cacttattta aaattgtccc aattgatcac aatgggtata
    13621 tccacaaact gttttattca gaaatggtta aagatgaaga tgtcatgtat tttgtgtcag
    13681 atggacgaaa aacttatcgc tttttatttg gagatacaat tctaaagact gataaacaag
    13741 gcaatgaata tctcacatac tccgttgaaa ataattttcc gccatttgca aaactagtta
    13801 tcgactacat tctaggtact tacacgtttt ttgagaataa actttatgac attcgatata
    13861 agcaatttaa attgattgat gattttacac ttcagactaa gtatggtttc aaagactctg
    13921 gtcacatttt agagatacta caaggtatcc acaaaacatt aaacatccaa ccaatcaatt
    13981 atattgaacc atatcagatt gcttgcaagg atttcataat cgaccttgaa aattctgaaa
    14041 tcattaacca accgcctttg caaaacgtat cttattttaa gtattacgaa gtagactaca
    14101 aaacagcaat aaacagtaag tctattgcag aagaatatct tgaatacgtt attgcagata
    14161 gcaattcgtt aaacaatgca atactacaat cttattttat cgcccaagtg gcgtgtggtg
    14221 ttagacctaa aaccaacttc ttcatctcaa aatctggagt aaggactggt aaagggttaa
    14281 ggcatatagc tttatctggt ctattcaata agattgatgt tgagctagat acattaaaaa
    14341 gtaatggatt tgaagcgtta caggcgtggg cgatgttctc gggtggagaa atggctctag
    14401 cgacggaaca gggagatatt caaggcaatg caatggagcg tgtacttaaa attattgcaa
    14461 cagagaaaac acacgttgca cgagcaattg ggcaaaatca gtcaattgtt aatttaacga
    14521 gtgttctgtg tattgacaca aaccgtactg ttgcgctgtc ggatgaaatg aatggacgaa
    14581 aggttttaat tcagtttaaa gatagaccaa aaactgaaac tgattatgaa agagagagcg
    14641 tattcaggaa atattggcta gcatttactg atcgtgataa aaatccaaaa atcgatggtt
    14701 gtatcggttt tctgttaaac tcactcgagc gtttccaaaa aattggtaaa tggtatcaat
    14761 ggaaagatgt tgaagtattt aatgatatcg atttagacga atttcaagtt gctttaataa
    14821 atgcattaca agaagtcgat tttgtacagc gaactgataa caaagaagtt attgacttat
    14881 cattacaagt ttacggaaaa agcaatcatg cagtaagtaa agccatatct gaaattggtg
    14941 tacgtagcag gtcgaagaaa gttaacggga aaacagtcag agggtatgaa atcgaaaata
    15001 aaacacgttt tgataaatat atcctttaaa aaggtaacgc atggtcacgc agagttacgc
    15061 aaaattttcc atgttgcgta acccactcaa tccctttagt accaaccgat ttcagccttt
    15121 attcaagaaa ggttacgcag taacaaacaa taagtctaat taatgattta t
//
LOCUS       DATRQA010000040        15108 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.040, whole genome shotgun sequence.
ACCESSION   DATRQA010000040 DATRQA010000000
VERSION     DATRQA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15108
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.040"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            128..1252
                     /gene="mutY"
                     /locus_tag="VBD27_000910"
     CDS             128..1252
                     /gene="mutY"
                     /locus_tag="VBD27_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO7024938.1"
                     /translation="MWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YSKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFQREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            1429..2022
                     /locus_tag="VBD27_000911"
     CDS             1429..2022
                     /locus_tag="VBD27_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7024939.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQTYKEILAVSSGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVATFFASRFIFTWLFHLS"
     gene            complement(2068..2382)
                     /gene="trxA"
                     /locus_tag="VBD27_000912"
     CDS             complement(2068..2382)
                     /gene="trxA"
                     /locus_tag="VBD27_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO7024940.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(2463..2963)
                     /locus_tag="VBD27_000913"
     CDS             complement(2463..2963)
                     /locus_tag="VBD27_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7024941.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRLDFPRPYEKWNIKPLIYKDTEGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLIL
                     GLCLFI"
     gene            complement(2967..5306)
                     /locus_tag="VBD27_000914"
     CDS             complement(2967..5306)
                     /locus_tag="VBD27_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO7024942.1"
                     /translation="MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDNFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(5391..5933)
                     /locus_tag="VBD27_000915"
     CDS             complement(5391..5933)
                     /locus_tag="VBD27_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO7024943.1"
                     /translation="MLSLLLLIIVIWHFYIGYSRGIFLQVFYVLMSMVSLMIASQFYQ
                     ELASQITLWVPYSNPVQGVEVYFFKDISKFQLSHVYYAGVAFVFIYSLSYLVGRLLGV
                     LLHLAPVEHFDSLQNNIISGFLAVLVCLLFMSMCLTILATVPMSFVQEKLWNSLFVRF
                     LINDLPFFSQFLVRTWAVGL"
     gene            complement(5936..6247)
                     /locus_tag="VBD27_000916"
     CDS             complement(5936..6247)
                     /locus_tag="VBD27_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024944.1"
                     /translation="METKINNSQKNRYKFVFGDKPLTLTTDKDNLFMEEIERVATEKY
                     EAIKEKLPNADNETIAILMAINALSVQLSREIDIEKMEDELNKLRSKTISDIKEKVSE
                     D"
     gene            6358..7251
                     /locus_tag="VBD27_000917"
     CDS             6358..7251
                     /locus_tag="VBD27_000917"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEO7024945.1"
                     /translation="MNTIVMQADKKLQEKIRTDLAQHHISNNNPYVVFSAKISGATVL
                     LYTSGKLVFQGSNASHIAQKYGFIEQKESCSSESQDIPIIGTDEVGNGSYFGGLAVVA
                     SFVTPKDHAYLKKLGVGDSKTLTDQKIKQIAPLLEKAIPHKALLLSPQKYNQVVSPNN
                     KHNAVSVKVALHNQAIFLLLQDGFEPEKIVIDAFTSSKNYQNYLKNEKNQFKQTITLE
                     EKAENKYLAVAVSSIIARNLFLENLNKLSDDVGYKLPSGAGHQSDKVASQLLKAYGIS
                     SLEHCAKLHFANTKKAQALLK"
     gene            7267..7860
                     /gene="lepB"
                     /locus_tag="VBD27_000918"
     CDS             7267..7860
                     /gene="lepB"
                     /locus_tag="VBD27_000918"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO7024946.1"
                     /translation="MKEFIKEWGVFILILSLFLLSRIFLWQFVKVDGHSMDPTLADKE
                     QLVVLKQTKINRFDIVVANEEEGGQKKKIVKRVIGMPGDVIKYKNDTLTINNKKTEEP
                     YLKEYTKLFKKDKLQEKYSYNPLFQDLAQSSTAFTTDSNGSSEFTTVVPKGHYYLVGD
                     DRIVSKDSRAVGSFKKSTIVGEVKFRFWPIRRFGTIN"
     gene            7989..10409
                     /locus_tag="VBD27_000919"
     CDS             7989..10409
                     /locus_tag="VBD27_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEO7024947.1"
                     /translation="MEVFFTGTIERIIFENASNFFKILLLEIEDTDSDFDDVEVIITG
                     TMADVIEGEEYTFWGTLTQHPKYGEQLQSVRYERAKPTSGGLVKYFSSEQFKGIGKKT
                     AQRIVELYGDNTIDKILESPEQLSTISGLSKINREAFIAKLKLNYGTEQVLAKLAEYG
                     LSNRAAIQIFDHYKEESLEVINENPYQLVEDIQGIGFKIADQLAEQVGIESDSPKRFR
                     AAIIHTLVESSMEQGDTYIEARTLLEKTITLLEEARQIELDPSIVAKELTNLIAEDKV
                     QHIGTKIFSNTLFFAEEGIKKNLQRILNQPLDKQLNHKDIDREIRDIQKSLNIHYDNI
                     QEKAIREALLSKVFILTGGPGTGKTTVINGIIEAYSELHHIDLNKNDIPIVLAAPTGR
                     AARRMNELSGLPSATIHRHLGLNGDSDYQSLDDYLDCSLIIIDEFSMVDTWLANQLFD
                     ALDSHTQVIIVGDSDQLPSVGPGQVLADLLNINALPHVKLEKIFRQSEESTIVTLANQ
                     MRQGFLPEDFTAKKADRSYFEASANIIPNMISKIVQSALKSGIEAHEIQILAPMYRGQ
                     AGINNLNLIMQNLLNPLKDNNQFTFNDINFRIGDKVLHLVNDTELNVFNGDIGYITDL
                     IPAKYTESKQDEIYMTFDGQEVIYQRKEWLKITLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRMLQRNLIYTAITRSKSKLILLGEIGAFDFAVKNEGAKRNTYLIERFENKQEIANS
                     QKIEDSSIDQKIDNTIINTSIPKTATPIEQTNLSKITYRLTEENYLTIDPMIGINQQD
                     ISAIFDSK"
     gene            10523..11005
                     /locus_tag="VBD27_000920"
     CDS             10523..11005
                     /locus_tag="VBD27_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024948.1"
                     /translation="MVSYEKVRRSLRTATITIIVLNSLSLVFRLFTGISVQLAKTEIN
                     KGNTGNLPKEHIEAVLSATTPFMLFVTALIVLVNIAIVIFCIKNLRAIKRNQMVNYLP
                     YYLGFAITVGLVILGFLTTKAPWAIAINIVFQAIFGLLYFHAYQKAQKLNERDLEETN
                     "
     gene            complement(11076..12185)
                     /gene="dinB"
                     /locus_tag="VBD27_000921"
     CDS             complement(11076..12185)
                     /gene="dinB"
                     /locus_tag="VBD27_000921"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEO7024949.1"
                     /translation="MGERTMLIFPLINDTSRKIIHIDMDAFFASVEERDNPSLKGKPV
                     IIGSDPRKTGGRGVVSTCNYEARKFGVHSAMSSKEAYERCPQAIFISGNYQKYRQVGM
                     EVRDIFKKYTDLVEPMSIDEAYLDVTENKMGIKSAVKLAKMIQYDIWNDVHLTCSAGI
                     SYNKFLAKLASDFEKPKGLTFILPDQAQDFLKPLPIEKFHGVGKRSVEKLHALGVYTG
                     EDLLSLSEISLIDMFGRFGYDLYRKARGINASPVKPNRVRKSIGSEKTYGKLLYNEAD
                     IKAEISKNVQRVVASLEKNKKVGKTIVLKVRYADFETLTKRMTLEEYTQDFQIIDQVA
                     KAIFDTLEESVFGIRLLGVTVTTLENEHEAIYLDF"
     gene            12355..14667
                     /gene="pflB"
                     /locus_tag="VBD27_000922"
     CDS             12355..14667
                     /gene="pflB"
                     /locus_tag="VBD27_000922"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEO7024950.1"
                     /translation="MATVKTNTDIFEQAWEGFKGVDWKEKASIARFVQANYAPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEETRFPMDTRVASISELPAGFIDKDNELIFGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYATTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLAVYGADYLMQEKVNDWNALNDIDEESIRLREEI
                     NLQYQALGEVVKLGDLYGVDVRKPAMNTKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAYDALYSGDPTFITTSMA
                     GMGADGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYAFRRYCMSMSHKHSSI
                     QYEGVSTMAKEGYGEMSCISCCVSPLDPENEDKRHNLQYFGARVNVMKALLTGLNGGY
                     DDVHKDYKVFDIDPIRDEVLNFDTVKANFEKSLDWLTDTYVDAMNIIHYMTDKYNYEA
                     VQMAFLPSHVRANMGFGICGFANTVDSLSAIKYATVKPIRDEDGYIYDYETVGDFPRY
                     GEDDDRVDSIAEWLLEAFHGRLAKHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKG
                     VYLNEDGSVNLSKVEFFSPGANPSNKAKGGWLQNLNSLSKLDFAHANDGISLTTQVSP
                     RALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLKDVYDKIMNGEDVIVRISGYCV
                     NTKYLTPEQKTELTQRVFHEVLSMDDALTN"
     gene            14768..15108
                     /locus_tag="VBD27_000923"
                     /pseudo
     CDS             14768..15108
                     /locus_tag="VBD27_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931877.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
BASE COUNT         5016 a         2852 c         2510 g         4730 t
ORIGIN      
        1 agaaaaaaat attgatgcaa catttttctt catcattaag tacgatctag tcaacatacc
       61 tctttgtcaa atcctctttc tttgatacaa ttaatctatg atagatttaa aagactatgg
      121 aattgagatg tggcctgaag atagaatcgc ttcttttcgt cgcacacttt tagggtggta
      181 tgaccaagaa aaacgtgatc tcccttggcg aagaactact aatccatact atatttgggt
      241 ttctgaaatc atgttgcaac aaacacaagt taatacggta atcccttatt ataaacgttt
      301 tctagagtgg tttcctcaaa taaaagattt ggcagacgct cctgaagaac aattactaaa
      361 ggcatgggag ggactaggct attattctcg ggtgcgtaat atgcaaaagg ctgcacagca
      421 agtgatggtt gattttggtg gcatatttcc tcacacttat gatgatattg cttcattaaa
      481 agggattggt ccttatactg caggtgctat cgccagcatc tcattcaact taccagagcc
      541 tgctgttgat ggcaatgtga tgcgtgtcat ggctcgtttg tttgaagtta attatgacat
      601 cggtgaccct aaaaatcgta aaatttttca agctattatg gaaatactaa ttgaccctga
      661 cagacccggt gattttaacc aagcacttat ggatctgggt acagacatcg aatccgctaa
      721 gacacctaga cctgatgaaa gccctattcg tttctttaat gctgcttatt tgaatggtac
      781 ttatagtaaa tatcccataa aaaatcctaa gaaaaagcct aaaccaatgc gaattcaggc
      841 ttttgtcatt cgtaaccaaa atggtcaata tctactggag aaaaatacta aagggagact
      901 tctagggggt ttttggtcat ttcctattat cgaaacgtca cctctttctc aacaattaga
      961 tttatttgat gacaatcaat ccaaccccat aatctggcaa acccaaaatg aaactttcca
     1021 aagagaatat caattaaagc cccaatggac tgataatcac tttccaaata ttaagcacac
     1081 ttttagtcac cagaagtgga caatagaact catagagggg gtggtcaaag caacagattt
     1141 acctaatgct cctcacctaa agtgggtagc aattgaggac ttttctctgt atccctttgc
     1201 aacaccacaa aagaaaatgc tagagaccta cttaaaacag aaaaatgctt aatcacaagt
     1261 gaactatgac ggttcacttt ttgtttacaa ttcctttacg ccgctgtaaa gttttcctca
     1321 gattatcttt taatacccta taaatagtgg tctatcccta atgtcaaata catttgatag
     1381 acccgttagc ttatcatcct tatactaata ttatgaaagg agatatttat ggaaattggt
     1441 cagcaaatta ttcgctaccg taaacaacaa gcgctctcgc aagaagaatt agccgagaaa
     1501 gtctatgtaa gccgtcagag catatctaac tgggaaaatg acaaaactta cccagacatc
     1561 catagcctct tactcctaag tcaaattttt caagtgagct tagaccaact aattaaagga
     1621 gacattgaga aaatgaaata taccatcact caggttgata aaaagaattt tgaacgagat
     1681 accaaagtaa tggtgacatt gatgatacta ttaatgattt caagttatcc attagtctat
     1741 ttcttagagt ggctaggact tggcattttt gttttattat cgataattac tatgacttat
     1801 gctaatcgtg tggaacgttt taagaaaaaa tatgatgtgc aaacttataa ggaaatccta
     1861 gctgtttcaa gtggaaaatt attagacgaa atcgaaaaac gggaagaaag agcaaaactt
     1921 ccttatcaga aaccccttat tgtcactgta tttttcctca ttactgttgc cacctttttt
     1981 gcaagtcgtt ttattttcac atggttgttc catttaagtt agtagataat aaaataaagc
     2041 tcagcgtgac actgggcttt attttattta actcaattca gcaataatag ctttcagttg
     2101 gtctttagta tgaacacctg caacttgttt aaccacttct ccgtcttttt taaacatcaa
     2161 tgttggaatt gacataatac caaactgacg ggcagtttcc ggattctcat caacatccat
     2221 tttcaaaatt ttgagttcat cttcatcgat ttcttgagat aactgttcta agattggtgc
     2281 ttgcatacga catggaccac accaagttgc ccaaaaatca atgagtacta aaccttcttt
     2341 agtttcttct acaaatgtag catctgttac ttctaatgcc atcttttttc tccatttcat
     2401 cttaatgata agcctattgt acaagaattt agttgttttt gaaactattt gctacgaact
     2461 cgctaaataa ataaacaaag ccctaaaatc aagccaataa ggtaacctac aataacatcc
     2521 ttaggatagt gaatacctgc tatcacacgg caaattgcca atagaaccga taatattaaa
     2581 caaactattc caaagtaaac ataatatcgt aacaaacaca tactaatcat cgtagctgaa
     2641 aaaacatgtc tgctaggcat tgaccttccc tcagtatcct tataaattaa aggtttaata
     2701 ttccattttt catatggtcg tggaaaatca aggcgtttcc taatgtaaga caaagctata
     2761 aagccaacag cagggataat taatagcctt aaccataatt gaaatgtcat cccttgccaa
     2821 agagtaaata tcagaaaaga tggatagagt atgtacatca ttcctgtgac tatttttagt
     2881 aaaaaattta aaagaattat taactgtggg gattttctaa aaggttgaga tagtttggca
     2941 taaaattgtt catatgattc cataatttac cctaacgtta caattgtggc gccagatccc
     3001 cctgcatttt gtggggcata agcaaaatgc ttaacgtgct tattacgacg aagatatttt
     3061 gtcactccct cacggataac gcctgtacca ataccatgaa tgatatcaac ttgtcccata
     3121 ttgttaagca atgcttgatc aataaaatta tctaactctt gcatagcttc ttcgtatctt
     3181 ttacctctaa gatcaagtcg agctcttggt ccagaactat cagccttttt aaccacatta
     3241 atctgtttac ttttgacttt ctgttcttct tggactctaa caagggtaaa ttcatcttgt
     3301 gttaatgtca ttttgataat tcccacttgt gcttcccaac gtccatcttt taattgacta
     3361 gttaaggtac ctcgctgtcc atagctagtc actataatat catcaccaat tctaggagca
     3421 cgagctgctt taattttttt agccttattt aagactttat tttttgataa atcaacttga
     3481 ggtgctaatt tttttatttg tgccttagca tctataattt cgtgaggttt taattggctc
     3541 ttatcattga gtttttttaa gatagtatca ctctcattca aagccatatc tacaatttct
     3601 tgagcttctt gatagatttt ttctaactct ttatcgcgct catgtgaaaa ttcattatag
     3661 agtttcttaa ccgcacgatt gaatttgagg ttttcttgtt caacttcttt aatatgatcc
     3721 agtcttctac gtgtctcaag tgtctgtgcc tctaactgtt caataatacg gttaacatct
     3781 gagtcagaat ctgtcatctg cttagcttgt ttaacaataa atggagctaa accaaggcga
     3841 gaagcaattt caaatgcatt tgatcgtcca ggaactcctt gcataaagcg atacgtaggg
     3901 ctaagcgttt cggcatcaaa ttccatgctc gcattctcta caaaatttgt ctcaatccca
     3961 taagctttta attctggata gtgcgtggtc gccatcgttt tgatattact taacctaagg
     4021 tgttctaaaa tagccatagc caaactagca ccttcttgag gatccgttcc tgctcccagt
     4081 tcatcaaaga ggactaaact attgtggtca gcctcgttta aaatactgac tatgtgcgtc
     4141 atatgactag aaaaagttga tagactttgt tcaatagatt gctcatcgcc aatatctgca
     4201 aagatattgt taaatactgc aattttacta cctttatccg ctaatactgg caaaccagac
     4261 tgtcccatta attgtgctaa accgagtgtt tttagcataa tcgtcttacc accagtattg
     4321 ggaccagtga tgacaattgc agttaaatct tgatcaaaat gtaagtcatt agccacaggg
     4381 ttacttaaca gaggatgacg aacattgatt aatgctaacg tgctgtcatt agaaatctca
     4441 ggtatcgtcg ccttattatc agacataaaa agatatttag cccttacaaa atcaagatgc
     4501 ccaagaatcc atgcattatt tctaatagtg gcgacatggg gtcttaacaa gtctgaaaat
     4561 gcgtgtaaaa tacgactttc ttcatgacgt tcgtcagctc taagctgcgt tatctcttcg
     4621 tttagtgtaa ctacagcacg aggctcaata taaacagtac ttcctgaaga agagatgtca
     4681 tgaaccacac cagaaatacg attccgataa gtatttttta ctggtaggac acttcgtcca
     4741 ctacgactag cgattagatt ctctgataaa agctctgctt tttccttaag catatcctgt
     4801 aaaatctgac gaacccgtcg ttcactgttt gttaattgac gacggatacg ctctaattct
     4861 ggactcgcaa aatgttctaa aaaaccacca tcattgatag cttgaaaaga cccttgcaga
     4921 ttagggaatt gttccaaatt ttcaaacaaa cgatccaaag attggaaaga aacattatcc
     4981 aaatcagaat aaaagtgaat catatccgaa gaactctgta aaactttttt gatagccaaa
     5041 agttcttgaa tattaagatc agctgaaagc tctaagcgtt ttaaactctc agagattgaa
     5101 cttaggctga ctatgccaaa tgagtgatgt tcaacaaaaa tctgttccat gtcagaaatt
     5161 tcattaaaac ttttttctat tttaggagcc tctgtcatcg gctccagctc tgataattct
     5221 tcttgtgatt gttctgtctt gagataaggt aatatcaatt ccttaacttt gttaaattct
     5281 aactgttcta aaatcttgtt attcatactc ttcattatac ctaaatttat cacaaaatga
     5341 caacgttaaa aaggttggaa ctcttactcc aaccttacta aacattatat tcaaagacct
     5401 accgcccaag tccttaccaa aaactgtgag aagaatggta agtcgttaat taaaaatcta
     5461 acaaacagac tattccataa cttttcttga acaaatgaca tagggactgt tgccagaata
     5521 gtcaaacaca tactcataaa taagagacaa accagaacgg ctaaaaaacc actaataata
     5581 ttattttgca agctatcaaa atgttcaact ggtgctaaat gtagtaaaac acctaacaag
     5641 cgacctacta agtaagataa actgtagata aaaacaaagg caacacctgc ataataaaca
     5701 tgactcaatt gaaatttgct aatatcttta aaaaagtaaa cttctactcc ttgtacagga
     5761 ttagaatacg gtacccataa cgtaatctga ctagctaatt cttggtaaaa ttgactagca
     5821 atcatcaaag ataccattga cattaacacg taaaagactt gtaaaaaaat acctctactg
     5881 tagccaatat aaaaatgcca aataacaata atcagcaaaa gcaatgatag catgattaat
     5941 cctcactcac tttttcttta atgtccgaaa tcgttttgga gcgtaactta ttgagctcat
     6001 cttccatttt ttcaatgtca atttctcgac ttagttgcac tgataaagca ttaattgcca
     6061 tgagaatagc aatcgtttca ttatcagcat taggtaattt ttctttaata gcttcatact
     6121 tttctgtagc tacacgctca atctcttcca taaaaaggtt gtctttatct gtcgtcaatg
     6181 tgagaggctt atctccaaag acaaatttat aacgattttt ctgactatta tttatttttg
     6241 tttccatact atacctctgc tagcattata ccgtaaaata gggctttcgt aaaattctac
     6301 cccttatcat tacttgtcta ttcccttatt cctctttttt atgatagaat attgtctatg
     6361 aacacaatcg tcatgcaagc agataaaaaa ttgcaagaaa agataagaac cgatttagct
     6421 caacatcata taagtaataa caatccttat gtggtatttt cagctaaaat atcaggagct
     6481 actgttcttt tatatacttc tggaaaactt gtcttccagg gttctaacgc ttcacatatc
     6541 gcccaaaagt atggttttat tgagcaaaaa gaaagttgtt cctctgagag tcaagatatt
     6601 cccataattg gtactgatga agtgggaaat ggctcttact ttggtggact ggcagtggta
     6661 gctagttttg taactcctaa ggaccatgct tacctcaaaa aattaggtgt tggtgattca
     6721 aaaacactta ctgatcaaaa aattaagcaa atcgcgcctc ttttggaaaa agcaattcca
     6781 cacaaagcat tattgttatc gcctcaaaaa tataatcaag tggttagtcc aaataacaaa
     6841 cataatgctg tctccgtaaa agttgcttta cacaatcaag ctatcttcct tcttcttcaa
     6901 gatggttttg aaccagaaaa aatcgttatt gatgctttta ctagctccaa aaactatcaa
     6961 aattatttga aaaatgaaaa gaatcaattt aaacaaacga ttactctgga agaaaaagct
     7021 gaaaataaat atctagctgt agcggtcagt tctattattg cacgtaatct attcttagaa
     7081 aaccttaaca aattaagcga cgacgtagga tataaacttc caagtggagc tggtcaccaa
     7141 tctgataagg tcgctagcca attactcaaa gcttatggta tctcttcgct tgaacactgc
     7201 gcaaaacttc attttgccaa caccaaaaaa gcacaagcat tattaaaata aatagcaggg
     7261 aaaattatga aagaatttat taaagaatgg ggtgtcttta tcctcatcct ctcacttttt
     7321 ttactatcgc gtatcttttt atggcaattc gttaaagttg acggacactc catggatcca
     7381 actttagctg acaaggaaca gctagtagtt ctcaaacaaa caaaaatcaa tcgattcgat
     7441 attgtagtgg ctaacgaaga agaaggcggc caaaagaaaa aaattgttaa acgtgtcatt
     7501 ggtatgccag gtgatgtcat caaatataaa aatgacacct taactattaa caataaaaaa
     7561 acagaagaac cttacctcaa ggaatatact aaattattta aaaaggataa attacaggaa
     7621 aaatattcgt ataacccact tttccaagac ctagcacaaa gctctaccgc tttcaccact
     7681 gacagcaatg gcagcagcga atttactact gtcgtgccta aaggccacta ctatcttgtt
     7741 ggtgatgacc gaattgtctc taaagatagt cgtgccgtcg gttccttcaa aaaatcaacg
     7801 attgtgggag aggttaaatt ccgcttctgg ccaattcgtc gttttggaac tatcaactaa
     7861 aggaaattaa aagttcacct gacaaaagtt gaaatagcat attatattga aaatagggag
     7921 tgacaaatcc atttaaaata gggatttaca atcactccct ttaactgttt attaaaaagg
     7981 aaggtacaat ggaagtattt tttacaggaa ccattgaacg cattatattt gaaaatgcca
     8041 gcaatttttt taaaatatta cttctggaaa tcgaagatac ggatagcgat tttgatgatg
     8101 tagaagtaat tattactggt actatggccg acgttataga gggagaagaa tatacttttt
     8161 ggggaacact aacccaacat ccaaaatacg gcgagcaact acaaagtgta cgctatgaaa
     8221 gagccaagcc tacttcaggt ggccttgtga aatacttttc tagcgaacaa tttaaaggaa
     8281 ttggtaaaaa gactgcccaa cgcatcgtag aactctatgg agataatacc attgataaaa
     8341 tccttgaatc ccctgaacag ttaagtacaa tctcaggact atcaaaaatt aatagagaag
     8401 cattcatcgc taaactgaaa ctcaattatg gaacagagca agttttagct aaactcgctg
     8461 aatatgggtt aagcaatcgt gctgctattc aaatatttga tcactataaa gaagaatcac
     8521 ttgaagtgat aaacgaaaat ccttatcaac ttgttgagga tatccaaggt atcggtttta
     8581 aaatagctga ccaacttgct gaacaagtcg gtattgaaag tgattctcct aaacgctttc
     8641 gtgctgctat tattcatact ttagttgagt cttctatgga gcaaggagat acctatattg
     8701 aggctcgtac tctcttggaa aaaaccatta ctctccttga agaagctcgt caaatcgaac
     8761 tagatccttc aattgttgct aaggaattga caaatttaat tgcagaagat aaagttcaac
     8821 atattggaac taaaattttt agtaatactc tattttttgc cgaagaaggc atcaaaaaaa
     8881 acttacaacg aatactaaat cagcctcttg ataaacaact aaatcacaaa gatattgacc
     8941 gtgaaatcag agatattcaa aaatctttaa atatccacta cgataatatc caagaaaaag
     9001 ctattagaga agctttacta agtaaggtat ttatcttgac tggtggccca ggaacaggta
     9061 aaacaacagt tataaatggt attattgaag cctattccga gttacatcat attgatttaa
     9121 acaaaaatga tattcctatt gtacttgctg ctcctacagg tcgtgctgct agacgcatga
     9181 atgaacttag cggtttaccg agtgctacta ttcataggca tttgggatta aacggagata
     9241 gtgactatca aagtctagat gactatctag attgttcctt aattatcatt gatgaatttt
     9301 caatggttga cacttggctt gctaatcaat tatttgacgc tttggactcg cacacacaag
     9361 ttattattgt cggagatagt gatcaattac cgtctgtagg tccaggacaa gttctagcag
     9421 atcttttaaa tataaatgca cttccacacg ttaaacttga aaagattttt agacaatcag
     9481 aagaatcaac tattgtgact ttagctaatc aaatgagaca gggctttttg ccagaggact
     9541 ttacagctaa aaaagccgat cgctcatact ttgaagcatc tgctaatatc atcccaaata
     9601 tgatttctaa aattgttcaa tctgcactta agagcggtat tgaagctcat gaaattcaaa
     9661 ttttagctcc tatgtatcgc ggccaagcag gtattaataa tcttaacctt atcatgcaaa
     9721 atctcctcaa tccattaaaa gataataacc aatttacttt taatgatata aatttccgaa
     9781 taggagataa agtgttacat ttagtcaacg atacagagct taatgttttt aatggggata
     9841 ttggttacat aacggacctt ataccagcca aatatactga gagcaagcaa gatgagattt
     9901 atatgacttt tgacggtcaa gaagttatct atcaacgcaa agaatggctt aaaataacac
     9961 tagcctatgc tatgagtatt cataaatcac agggttctga atttcaagtt gttattctgc
    10021 caataacgag acagagtggt cgaatgttac agcgaaacct tatttataca gctattacga
    10081 gatctaaaag taaactaata cttttaggag aaattggtgc atttgatttc gctgttaaaa
    10141 acgagggggc aaagcgcaat acttatctga tagaaagatt tgaaaacaag caagaaatag
    10201 ctaattctca aaaaattgaa gatagctcaa tagatcaaaa gattgacaac acaataatta
    10261 atacgtccat tccaaaaaca gcaacaccaa ttgagcagac taatctttca aaaataactt
    10321 atcgcctaac tgaagaaaat tatttaacaa tcgatccaat gattggtatt aatcaacaag
    10381 atataagtgc aatttttgat agtaaataaa ttttttcata aaatcttgaa aactcttcca
    10441 aatatgttat aatatcattg tattaagaac gtagttcaaa tttaatactt ttattaaggt
    10501 ttatttagaa aaaggagata ttatggtttc atatgaaaaa gtccgccgct ctctgcgcac
    10561 agctacaatc actatcattg tattaaatag tttgtcttta gtattcagat tgtttacagg
    10621 aatttcagtg cagctagcca aaactgaaat taataaaggg aacacaggta atcttcctaa
    10681 agaacatatc gaagcagtct tgagcgccac aacaccattt atgctatttg tcactgcttt
    10741 aatagtatta gtcaacattg ccattgttat tttctgtatt aaaaatttaa gagcaattaa
    10801 aagaaatcaa atggttaatt accttcctta ttaccttggc ttcgccatta ctgtaggact
    10861 tgttattttg ggttttctaa ctactaaagc accatgggca atagctataa atatcgtctt
    10921 ccaagctatt ttcggtcttc tttatttcca tgcttatcaa aaagcacaaa aactcaacga
    10981 acgtgatctt gaagagacta actaaaagta aaagcactag aatctagtgc ttttttcttt
    11041 tcaaagagag ttaaatatag tcatcgtccc aacttttaaa aatctaaata gatagcttca
    11101 tgttcatttt ccagggtcgt tactgtaaca cctaagagtc gtatgccaaa aactgactct
    11161 tctaaggtat caaatatcgc tttagcaact tgatcaatga tttgaaaatc ttgagtatac
    11221 tcttctaatg tcatacgttt cgtcaatgtt tcaaaatccg catatctaac ttttaaaacg
    11281 attgtcttac caactttctt atttttctcc agagaagcaa caactctctg aacgttcttt
    11341 gaaatctcag ctttgatatc tgcttcgtta tatagtaatt tcccataggt tttctcactt
    11401 cctattgatt tacgaactct attaggtttt accggggaag cattaatccc tcttgcctta
    11461 cgataaaggt catacccgaa acgaccaaac atatcaatta aagaaatttc agataaggaa
    11521 aggaggtcct caccagtata aacccctaaa gcatgtaatt tttcgacgga gcgtttacca
    11581 acaccatgaa atttctctat tggtaagggc ttcaagaaat cttgagcttg atctggtagg
    11641 atgaaggtaa gtccttttgg tttctcaaag tcagaagcca attttgccaa aaatttattg
    11701 taggaaatac ccgctgaaca ggtcaaatgg acatcattcc aaatatcgta ttgtatcatt
    11761 ttagcaagtt taacagctga ttttattccc attttatttt cagttacatc taaataagct
    11821 tcgtctatac tcatcggttc aaccaaatcc gtatactttt taaatatatc tcgtacttcc
    11881 attcctactt ggcgatattt ttggtaattt cccgaaataa agatagcttg agggcatcgt
    11941 tcatacgctt cttttgaaga catagctgaa tgaactccaa actttcgagc ctcataatta
    12001 caagtagaaa caacccctct gccgccggtt tttctgggat cagaaccaat aataacaggc
    12061 tttcctttaa gagagggatt gtcacgctct tccacagatg caaaaaaagc atccatatca
    12121 atgtggataa ttttacgcga cgtatcattt attaaaggaa aaattaacat tgttctctcc
    12181 cccattctct ctactccatt ataacagatt acaacttaat gatacagatt aaaaaaactg
    12241 tattataaaa gaatcataat tttgtcaaga aatatgcttg cgaaaacgtt tactttttcc
    12301 taaaatggat atgtaaacaa aattaaatct gtcacaatgt ttaaggagaa aactatggca
    12361 acagttaaaa caaacacaga tatctttgaa caagcttggg aaggctttaa aggtgttgat
    12421 tggaaagaaa aagcaagtat tgctcgcttc gttcaagcaa actatgcacc ttacgatgga
    12481 gatgaaagct tcttagctgg agcaactgaa cgttcacttc acatcaaaaa agtgattgaa
    12541 gaaacaaaag ctcactatga agaaactcgc ttcccaatgg atactcgtgt tgcttcaatt
    12601 tctgaattac cagctggttt tatcgacaaa gataacgagc ttatctttgg tattcaaaac
    12661 gacgagcttt tcaaattgaa tttcatgcct aaaggtggta tccgtatggc ggaaactaca
    12721 cttaaagaaa atggttatga acctgatcca gctgttcacg aaatcttcac taagtacgct
    12781 acaacagtaa atgatggtat cttccgcgct tatacttcta acattcgtcg tgctcgccat
    12841 gcacacactg ttactggtct tccagacgct tattcacgcg gacgtatcat tggtgtatat
    12901 gctcgtctag ctgtttacgg tgctgactac ttgatgcaag aaaaagttaa tgactggaat
    12961 gcattgaatg atattgatga agagtcaatt cgtcttcgtg aagaaatcaa ccttcaatac
    13021 caagctcttg gtgaagttgt aaaacttggt gacctttacg gcgtagatgt tcgtaaacca
    13081 gccatgaaca ctaaagaagc cattcaatgg gtgaacattg ctttcatggc tgtatgtcgt
    13141 gttatcaatg gtgctgcaac atcacttgga cgtgttccta ttgtccttga tatctttgca
    13201 gaacgtgacc tcgctcgcgg tacattcaca gaatcagaaa tccaagaatt tgttgatgat
    13261 tttgtcctta aactccgtac tgtaaaattt gctcgtacta aagcttatga tgccctttac
    13321 tcaggtgacc caacctttat cacaacttca atggcaggta tgggagctga tggacgtcat
    13381 cgtgttacca aaatggacta tcgtttctta aatacacttg acaatatcgg taactcacca
    13441 gaaccaaact taactgttct ttggtcagat caattaccat atgctttccg tcgctactgt
    13501 atgtcaatga gtcataaaca ctcttcaatc caatatgaag gtgtatcaac aatggctaaa
    13561 gaaggctacg gtgaaatgtc atgtatttca tgttgtgtat ctccacttga tccagaaaac
    13621 gaagacaaac gtcacaacct tcaatatttt ggtgctcgtg ttaacgttat gaaagcactt
    13681 cttactggtc ttaacggtgg ttatgatgat gttcataaag attacaaagt atttgacatc
    13741 gacccaattc gtgacgaagt actcaacttt gacactgtaa aagctaactt tgaaaaatct
    13801 cttgactggt tgactgatac ttatgttgat gcgatgaata tcatccacta catgactgat
    13861 aaatacaact atgaagctgt acaaatggca ttcttacctt cacatgtacg tgctaatatg
    13921 ggatttggta tctgtggttt tgcaaatact gtcgattcat tatctgcgat taaatacgct
    13981 actgttaaac caatccgtga cgaagatggt tacatctacg actacgaaac agttggtgac
    14041 ttcccacgtt atggtgaaga tgatgatcgt gttgactcaa tcgctgaatg gttacttgaa
    14101 gccttccatg gacgccttgc taaacacaaa ctttataaag atgctgaagc aactgtttct
    14161 cttcttacaa tcacatcaaa cgttgcttac tctaaacaaa ctggtaactc accggtacat
    14221 aaaggagtat atctcaatga agatggtagt gtaaaccttt ctaaagtaga attcttctca
    14281 ccaggtgcta acccttctaa caaagctaaa ggtggctggt tacaaaactt gaattcactt
    14341 tctaaacttg atttcgctca tgcaaatgat ggtatttcat tgacaactca agtttctcca
    14401 cgcgctcttg gtaaaacatt tgatgaacaa gttgataact tggtaacagt ccttgatggt
    14461 tactttgaaa acggtggtca acacgttaac ttgaatgtca tggaccttaa agatgtctat
    14521 gacaagatta tgaatggtga agatgttatc gttcgtatct caggttactg tgtcaatact
    14581 aaatatctaa ctcctgagca aaaaactgag ttaacacaac gtgtcttcca tgaagtactt
    14641 tcaatggacg atgcattaac taactagttt gtattcgatg aagtaggttt gattatatca
    14701 aacctacttt tatctatatt atcaatagta ctaattactt ctttatataa cctcaaggag
    14761 gggcctaatg ttaaatcaca aatttcttca agttctaaaa tatgagggtg tcgtttcgat
    14821 aacaagctgg accgaattag ctcctcacgt tacaaacact tggaatagct acctgactat
    14881 tacagatgac cagcgtatat tagctcctgc agcaggaatg actcatcttg aaatgatctc
    14941 aataataatt ctaaaattat tatgactctg ggcagccgtg aagtcgaggg tcgtgatggt
    15001 tatcaaggta caggctttag aattgaagga actgctaagc tattagaagc aggaagcgat
    15061 tttgaaattg ttaaagaaaa ataccctttt ctgcgtaaag tattggaa
//
LOCUS       DATRQA010000041        14744 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.041, whole genome shotgun sequence.
ACCESSION   DATRQA010000041 DATRQA010000000
VERSION     DATRQA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14744
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.041"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            699..786
                     /locus_tag="VBD27_000924"
     tRNA            699..786
                     /locus_tag="VBD27_000924"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:733..735,aa:Ser,seq:gct)
     gene            complement(1008..1385)
                     /locus_tag="VBD27_000925"
     CDS             complement(1008..1385)
                     /locus_tag="VBD27_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEO7024951.1"
                     /translation="MLVYPAIFTQDSDYIMVTFPDVPEAITQGEDFQEAYEMAIEVLG
                     FALEDYTDYPKASPVSVLKEQYHDSDIALIGIDMIAYMKKYHSKKVRKNVTIPEWLNN
                     AAEDKNLNFSQVLTEALELKLQA"
     gene            complement(1438..1623)
                     /locus_tag="VBD27_000926"
     CDS             complement(1438..1623)
                     /locus_tag="VBD27_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEO7024952.1"
                     /translation="MPMTPKQMIKLLKKNGFYEISQNGSHKKLRDDLGHQTIVPMHNK
                     DLGKGLEDTILKQAGLK"
     gene            1732..2223
                     /locus_tag="VBD27_000927"
     CDS             1732..2223
                     /locus_tag="VBD27_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEO7024953.1"
                     /translation="MGKLTLKQQKFIDEYIICGNATDAAIKAGYSKKTAGQIGEQNLK
                     KLEIKQAIQSRMKVLEKSSIATADEVLRVFTQILRQELTEEVTELNQITGEFVTIEKQ
                     PSIAEVIKAGSELMKRYPTKWELEKLKLEIEKLKAQVVGDEEQEDKLVAFTKALGEVL
                     DDR"
     gene            2213..3427
                     /locus_tag="VBD27_000928"
     CDS             2213..3427
                     /locus_tag="VBD27_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEO7024954.1"
                     /translation="MIVDFTPKQWQVLEQIRHNDFFICGLHGAKRSGKTYVNNITFLN
                     ELVRVRRIADKLGVEEPMYILAGTSSTSIHNNILQDLYNTFDIEPKYDKHGAFVLAGV
                     KVVQVYTGSISGLKRARGFTAYGAYVNEASLANETVFKEIISRCSGEGARIVWDSNPD
                     NPNHWLKTDYIDSDDDMIIDFSFKLDDNTFLSKRYIESIKAATPNGKFYDRDILGKWT
                     VAEGAIYADYDKSIHEVDDLPEMIRYFAGVDWGYDHYGSIVIIGEDSEGNRYLVDGIA
                     EQYKEIDWWVDRAKEFMQVYGDIVFWADSARPEHVARFRRERIKTRNASKEVVAGIEH
                     VAKLLKENRLFIKRGVIPRFFDEIYQYKWKPNSTKDEPLKEYDDVLDALRYALYSDFR
                     PKTIVKTFKGGI"
     gene            3427..4752
                     /locus_tag="VBD27_000929"
     CDS             3427..4752
                     /locus_tag="VBD27_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO7024955.1"
                     /translation="MPELFIVPSDTEMTKDLLNELIQKHKSFNADYLVYKQLYEGSHA
                     ILGQKPKEQYKPDNRLVVNFAKYIVDTFNGYFIGVPIQTSHENKQVSNYLELLDGYND
                     QDDNNAELSKICSIYGHGYELVFNDENAEVGITYLTPLEAFIVYDDSIRQKPLFAVRY
                     FYNKDGILEGSYSDASNITYFKDGEKGLEIGESEKHPFDGVPMIEYVENEERQSLLAS
                     VVTLINAFNKAISEKANDVEYFADAYLKILGAELDDETLKSLRDTRIINLKDPDVKQL
                     VVEFLQKPDADATQEHLLDRLEDLIFRTAMVANISDESFGTASGIALRYRLQAMDNLA
                     KTKERKFMSGMNRRYKLIASYPISKIGPKDWIGVKYKFTRNLPANLLEESQIAGNLAG
                     IVSEETQVGVLSIVENPQKEIEKKNSDRPTLISRKAGGLNGQDTTTVLE"
     gene            4721..5647
                     /locus_tag="VBD27_000930"
     CDS             4721..5647
                     /locus_tag="VBD27_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEO7024956.1"
                     /translation="MAKTPQQYWNDRQKQLWANLETSERALQTKLSKCYADEAKSLEK
                     EIGAYFSKYGKGNVIEYRNLLQQLSKADKDLLYRDCERFAKKYPQHADLMPVRTSIYK
                     LDRLQGLELSIKMQQLEIGSIEEAELTKHLTTAFKKGYQETAKTIGFQADKVSAELFI
                     NNDWTGKGNFSSSIWTNKDKLVDYLTNDFKTAIIRGDSFDKVVKQMSERFTVRSQSDI
                     TRLIMTEGTYVNNQAMMKPFEDSGLYDEFEFVAVLDGHTSSLCKGLDGQKFPMNSKQV
                     GINFPPMHANCRSTFAMVIPDDYVDRYEKENN"
     gene            5781..6107
                     /locus_tag="VBD27_000931"
     CDS             5781..6107
                     /locus_tag="VBD27_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024957.1"
                     /translation="MKVYDKYLNEEKDRLVEVHTEWLKEIDGYIKNQEDKIEKSELKL
                     LETEEIFIERFERLERRIKELENHDKISFKIDENELVQKLNDLTERSIALVKARVLNG
                     ITGKES"
     gene            6222..6791
                     /locus_tag="VBD27_000932"
     CDS             6222..6791
                     /locus_tag="VBD27_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEO7024958.1"
                     /translation="MESEEILEQSGAQEEAKEQTFDDILSDPKKQAEFDKRVAKAIDT
                     AKSKWQAETEEKENEAKRLAKMNAEQKAQHEKAKLEERIAELEAERTLSEMKSAARTM
                     LSEANISVSDALLSQLVSTDADKTKNAVEAFSEAFSEAVEKEVKERLKSPTPKKSNGN
                     SGLTKEQILAVKDTAERQKLIAENIGLFQ"
     gene            6810..7700
                     /locus_tag="VBD27_000933"
     CDS             6810..7700
                     /locus_tag="VBD27_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEO7024959.1"
                     /translation="MVTSRTYPEENLIKSTDLKYPITIDVTNKFQENISKLLEMLGVT
                     RKISVSEGMTLKTYAGYDVTLADGTVPEGEVIPLSKVARKTHSEKKIELKKYRKATTG
                     EDIQMFGSNEAVTNTDNALVRQLQKKIRTDFVTALKTGTGEQTALGAGLQGALASAWG
                     KLQVLFEDYGSERAIVFANSLDVAEYIAKAGITTQTAFGLTYLVDFTGTVIISTNDVA
                     KGEIWATVPENIIFAYINPNNSELAKEFNLYGDPTGYIGMNHFQENTTLTIQTLLVSG
                     MLMYPERIDGIVKVTLTPGI"
     gene            7712..7993
                     /locus_tag="VBD27_000934"
     CDS             7712..7993
                     /locus_tag="VBD27_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEO7024960.1"
                     /translation="MAYTVKARFFDLLDNSFLYEVGDIFPRKGYEPSKERLESLLSSN
                     NAYHKPFISASESLHDMKVTELKEKAKDLGIELPAKANKEEIIELIEKK"
     gene            8002..8343
                     /locus_tag="VBD27_000935"
     CDS             8002..8343
                     /locus_tag="VBD27_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013794250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024961.1"
                     /translation="MSANEDILARVKIRLSDEANVNPLLLSELVQTAIDRINLKVGDV
                     IFNPIFNSIAVDVVVKMYRRLYYEGISSEDADTIRTTFVNDLLAEYGEELASYKKDRL
                     AILNKKVVRFL"
     gene            8340..8651
                     /locus_tag="VBD27_000936"
     CDS             8340..8651
                     /locus_tag="VBD27_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024962.1"
                     /translation="MRYTTFNLVTDVKTGKKDALGNDIITKTESISHKGRFTEWHADD
                     EIVYGRDLTSSTRKMISNTITLGQAKKASQVKLDGKYYDIKSVKDLGRWRLLLINGYR
                     L"
     gene            8648..9049
                     /locus_tag="VBD27_000937"
     CDS             8648..9049
                     /locus_tag="VBD27_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024963.1"
                     /translation="MKFTIKGTEKLEERFRAMNAIRWDGVVNKSLTQMFNRGARPPGT
                     PVGKQTKNHSAGELRRSRRVKKISSKDGSYTGSFYYTKDYAPHVEYGHRIVRKGKQVG
                     YANGKKYLFNNVQRQRQIYFKDMLAELRKKR"
     gene            9053..9463
                     /locus_tag="VBD27_000938"
     CDS             9053..9463
                     /locus_tag="VBD27_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5072 family protein"
                     /protein_id="HEO7024964.1"
                     /translation="MLKKLGLTDLHASLKETIEKNTGVVAYDSVPEDAPSPFYFIEIV
                     DKRPEDTKVMWCEVFTIWIHAIAEKGKSKIAIYKMIEDLEEALTMELALPDGVEVLRQ
                     VETGMQSLQEDETGENHAIVAYEIKVAYGFKTKI"
     gene            9476..9982
                     /locus_tag="VBD27_000939"
     CDS             9476..9982
                     /locus_tag="VBD27_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEO7024965.1"
                     /translation="MGTEFDSGVYCNFDASSTKAIAGKDIILAIFDATGAKLLAISGQ
                     QGLTINRSADSIEVSSKDTKGGWKSKIAGMKEWSIDNDGLYIPSDETHKELGKAFEAS
                     NPVCIKVVNGKTKKGMFGGLAYISDYSLEAPYDDGMTYSISLDGNGALVDLSTLTEQA
                     DLVVAMPE"
     gene            9998..10315
                     /locus_tag="VBD27_000940"
     CDS             9998..10315
                     /locus_tag="VBD27_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024966.1"
                     /translation="MEKEIIKADEKQYELKYNEKTIETVEALTGKAFMDVIVNNKGML
                     SLSMLRQYFANALYAVEGGRVSSEQGSNIFTKVLNTKGYAYVNMLVINTIQRDCPFFF
                     LGA"
     gene            10288..10749
                     /locus_tag="VBD27_000941"
     CDS             10288..10749
                     /locus_tag="VBD27_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024967.1"
                     /translation="MPFFLPRRLVDFEYLGGSGDSTDVEYDRLASQYHKDIDFAFYFV
                     NFGTTKSEFLELTRREKAFIRKAWEDKQVRESELMRNAVLNAVSNAMRKKSAKFVDLW
                     KRQQQPANMEIVNAHLEIVSISEETEGKSWVDAIYKANNLKTPKGEEVGNG"
     gene            10742..12934
                     /locus_tag="VBD27_000942"
     CDS             10742..12934
                     /locus_tag="VBD27_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024968.1"
                     /translation="MADYTLGVQVTGDASSMQKEFDKAQKAVEALKKKTDEANKTASG
                     TFSEMSKSIGDTSEKLRSFGDKMSEAGKKIADVGSNMTKWITTPVVGAVGYAVKAYAD
                     LEQAVGGVETLFKSSSKEVISNAEKAYKTAGISSVKYMEQVTSFSASLLQGLGGDTAK
                     AAKVADKAIIDMSDNANKMGTSIELIQNAYQGFAKGNFTMLDNLKLGFGGTKEEMQRL
                     LEEAEKVTGIKYDINNLSDIIEAIHVIQTELGITGTTAKEAESTISGSFNQMLSSLNN
                     LSAGLGDKNADIKKLMNELEQSTRTFVINVSNVLKTIWDNLPLEPWQKWTIAVVALAG
                     PVLLIFGKLTVGIGNLITAFGTIGEAISSIIGWFGTLTAEGGALAGVFGSLGAGPIIA
                     IFAGIALAIGLVVLAIKDLWDNSEDFRTAVTEIWASISTMLTNVWDSFLLPVFEAIKA
                     IILSIVDESLIPLYDSFKQSLEDIAIELSTFLKIAEPFISTLADLFGKIIPPILEILG
                     ANFKCTFAIISNVMTVAFQTISGIVKNLLKVLGGIITFITGVFTGNWRKAWQGVVNML
                     GGIFGGIGVIMKGAINGVIGVINGAIGGINSLIGTVNKIPGVDIGTIGSIPYLARGTD
                     NWQGGFARINEGGRGELAYMPSGAVVVPHDVSMKYAKESARAQSGMIMVSGNDEIAKN
                     ALKLANEAVKRPVILRVNGRDLAVTTGKDFANYQERQTVTLKRMRGEL"
     gene            12945..14465
                     /locus_tag="VBD27_000943"
     CDS             12945..14465
                     /locus_tag="VBD27_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO7024969.1"
                     /translation="MTFNGVDLSGLINIMSINRDIGNEVTLTTDDSPNFGSHVQKSKI
                     HPKVISVDFYIKDKSSDYALNQLKHRLAGVFNVNKDVRITFSDEPDKYYLGRPINKIS
                     AGDPIAWLSLVTLELFIPDGVAHSTTYKKFLDYTQDGNKLTFKLQNEGNTNALPIIKI
                     KHNSENGYIGIANETGAFALGSSEEEDGTIVHRNETLFDYSKAIAQALDGAPNVAKLN
                     HMPPTYDTELKRKRIDNILGSGKGGEYVVIGNRGTTPGYTEHVGTRTFIINPDSNGEY
                     TLNEHLWWQQIFIATAQDQKGFLKLCVTGIDDEGNDEFLYGIETYKRKNGFETEYNFF
                     ALDDDGVGWRFYKQFKFQADRNYHNPFSMNRSRAVEIFREEDKFRIYFNGAHHHVTVP
                     SLKGKKSRKIHLAMGTCSDSSKYINYNLFEKVNFEKMGVSHYNNIVNKYQPSDEVVIN
                     FENDTVKTKELNSLQDMVLGSQPISIPPGESELVIQLSKFSQSAPNVEILMEERWL"
     gene            14456..>14744
                     /locus_tag="VBD27_000944"
     CDS             14456..>14744
                     /locus_tag="VBD27_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024970.1"
                     /translation="MVVITLVIHDAKLHPVLLLDNDKQGALNYYDDLWTRQLTNGSSV
                     FEFSVYKKTLEGDNPLNHKYQVLNDQAFVSFVHNDKVQLFNIMRVEETETTI"
BASE COUNT         5023 a         2415 c         3188 g         4118 t
ORIGIN      
        1 gtggcttagc acagataatc cgtagcgata tgggaaagct tttgtaggtg tatcaacaag
       61 agcgccagta atggtcaatc taagcaatcc aatctttatg taatcagtaa tggttatagc
      121 acacgaagtc aaagcgcaat accagagcca atcggtgagg tgctgtgtca gcagtacgtg
      181 agacgagtgt gtaggaggca taaactgacg aagttcaagg gtggtaccaa gagcggtcag
      241 catgttgtgc aacgctggga ggatatccca gtcaaacaca acaagtcaag gtgtaccagc
      301 accgagataa caagtaggcg ttgcgcattt tgttgctcaa aaggcgacga aacgcaaggc
      361 aatgcacgtc tgcgatacac gaaaacaatg ctatttgtta aaaatattgg aataaagcaa
      421 aagtcattgc ccgtcgcaaa cgaaagtgtg cttcggtagc taggctacct gctagagtct
      481 cgcaaggata atagcgaagt caaagagtaa agcagcttag acctttagcg gggttttcgt
      541 taattgaaaa atggcttagt agtctgcggc gtaacgagtg gttagcgata ctaaccgcgc
      601 atggttgtta cttgaaggga tttgagtgga taaaaaacta aaacataagg ttttgaaaga
      661 caactgacta aacgtgtaat ctcagcgtgt ttacatttgg aaagttactc aagtggttta
      721 agaggacagg ttgctacctt gttaggcgtg taaaagcgtg cgtaggttcg aatcctacac
      781 tttctatata ttaattgcaa acaaggtcgc aaccttgctt gtggtgtggg aacaatggtt
      841 ctgtgtcggt tcgattccga ctgttcctgt tcgataaata gaaatgtccc agaatggggt
      901 aggcaatcgg cttagcattc attcgctatt tatctatggt taaccaatta gtcatcacat
      961 tgtggtggct ttttattatg taaaaaaaag aaccacaaca gtggctctta tgcttgtaat
     1021 tttaattcaa gtgcttcggt aagaacttga gaaaagttga ggtttttatc ttcggctgcg
     1081 ttgttcaacc attcaggaat agtcacgttt ttgcgtacct tcttagagtg atattttttc
     1141 atgtaggcaa tcatatcaat gcctattaag gcaatatcag aatcatgata ctgttctttt
     1201 aaaacggaaa cagggctagc ttttggatag tctgtatagt cttcaagggc aaaacctaag
     1261 acttcgatag ccatttcgta agcttcttga aagtcttcac cttgagtgat tgcttcaggg
     1321 acatctggaa atgtaaccat gatataatct gagtcttgtg tgaatatagc tggataaact
     1381 aacataataa ttctcctttg attattgtga gataagcaag ccatcttgtt aagcggatta
     1441 tttcaaaccc gcttgtttta agatggtatc ttcaagaccc ttaccaaggt ctttattgtg
     1501 cattggaacg attgtttggt gtcctaagtc atcacgaagt tttttatgac taccgttttg
     1561 actaatttca taaaacccgt tctttttaag caatttaatc atttgcttag gggtcattgg
     1621 catattgctt acctcacttt ctatacttat attatacaca taaaagacat gtttgtcaag
     1681 taaaatacgc ataaaatact tattttttta aaacggagga ggtggtggaa aataggtaaa
     1741 ttaacactaa aacaacaaaa atttatagat gagtacatca tctgtgggaa tgcgactgat
     1801 gcagcgataa aagcggggta tagtaaaaaa accgctggtc aaattggcga gcaaaacttg
     1861 aaaaaacttg aaataaagca agctatacag agtcgcatga aagtcttaga aaagagttcg
     1921 atagcgacag ctgatgaagt tcttagagtg tttacacaaa ttcttagaca ggaattaact
     1981 gaagaagtaa cggaacttaa tcagattact ggtgaatttg taacgataga gaagcaacca
     2041 tcaatagcag aggttataaa agcaggaagc gaattgatga aacgctatcc aactaagtgg
     2101 gaacttgaaa aacttaaact tgaaattgag aaacttaaag cacaagttgt tggtgatgaa
     2161 gaacaggaag ataaattggt tgcctttact aaggcgcttg gagaggtttt agatgatcgt
     2221 tgatttcact cctaaacaat ggcaagtgct tgaacaaatc agacataacg acttctttat
     2281 atgcggtctt cacggcgcta agcgttcggg taaaacctat gttaacaaca ttacttttct
     2341 taatgagctg gtgagggtgc gtaggatagc tgacaaacta ggcgttgaag aaccaatgta
     2401 tattttggct gggacttcgt caacatctat tcacaacaac attttacaag acctgtacaa
     2461 tacctttgat attgaaccta aatatgataa acatggtgct tttgttctag ctggagtcaa
     2521 ggtggtgcag gtctatactg gttctatatc ggggcttaaa cgtgctcgtg gttttacggc
     2581 ttatggtgca tatgttaacg aagcttcact tgctaacgag accgtgttca aagaaatcat
     2641 ttctaggtgt tctggtgagg gtgcaaggat agtttgggat agtaacccag ataaccctaa
     2701 ccattggctc aaaacggatt atattgactc tgacgatgat atgattattg attttagctt
     2761 taaattagac gataacacat ttttgtctaa acgatacatc gaatctataa aagctgcaac
     2821 gcctaacgga aaattctatg accgtgatat tcttggcaag tggacggtcg cagaaggtgc
     2881 tatctacgct gattatgata aatctatcca cgaagttgat gacctcccag aaatgatccg
     2941 ctactttgca ggtgtcgact ggggctatga ccattatggc tctatcgtga ttattggtga
     3001 ggatagcgaa ggtaatcggt atcttgttga tggcattgct gagcagtaca aggaaattga
     3061 ttggtgggtt gatagggcta aagagtttat gcaagtttat ggagatattg ttttttgggc
     3121 tgacagcgct agaccggaac acgtcgctcg tttcagacga gaacgcatca aaacaagaaa
     3181 tgctagcaaa gaagtcgttg caggtattga gcatgtcgct aaacttctaa aagaaaacag
     3241 gttatttatt aaacggggcg tgatacctcg tttttttgat gaaatatatc aatataaatg
     3301 gaagccaaac agcacaaaag acgagccgct aaaagaatac gatgatgtgc ttgatgcttt
     3361 gcgttatgca ctatattcag attttagacc taaaactatc gtcaaaacat ttaaaggagg
     3421 tatctagtgc cagaattatt tattgtgcca tcggatacag agatgacaaa agatttgtta
     3481 aacgagctaa tccaaaaaca caagtcgttt aacgctgatt atttagttta taagcagctg
     3541 tacgagggtt ctcacgctat tttgggacaa aaacccaaag agcaatacaa gcctgataat
     3601 cgcttggttg ttaactttgc aaaatacatc gttgacacgt ttaacggtta ttttatcggc
     3661 gtgccaatcc aaacgagcca cgagaataaa caagtcagta attatttaga gttgctagac
     3721 ggatataatg accaagacga caacaatgca gagttatcaa agatttgtag tatttacgga
     3781 cacggttatg agttggtttt taatgacgag aatgcggagg tcggaattac ttatctgaca
     3841 ccccttgaag catttattgt ttatgacgat tccatcagac aaaaaccgtt atttgctgtg
     3901 cgctattttt acaataaaga cggcattttg gaagggtctt actcagacgc tagcaacatc
     3961 acttatttta aagacggcga aaaggggctt gaaattggag agagcgagaa acatccgttt
     4021 gatggtgtgc caatgatcga atatgtcgaa aacgaagagc gacaaagctt gctagctagc
     4081 gttgtgacat tgattaatgc gtttaataaa gctatctccg aaaaagccaa tgacgttgag
     4141 tatttcgcag acgcttatct taaaattttg ggtgctgagc tagacgacga gacattaaag
     4201 tctcttagag acacccgcat tatcaatcta aaagaccctg atgtgaagca attggttgtt
     4261 gagtttttac aaaagccaga tgccgatgca acgcaagagc atttgctcga ccgattagag
     4321 gacttaattt tcaggactgc tatggttgct aatatcagtg acgaatcgtt cggtacagct
     4381 agcggtatcg ctttacgtta tcgtttgcaa gctatggata acttagctaa gacaaaagag
     4441 cgtaagttta tgagcgggat gaaccgcaga tataagctta ttgctagtta tcccatatcg
     4501 aagataggtc ctaaagattg gattggtgtt aagtataaat ttactcgcaa cttgccagcg
     4561 aatctcttgg aggagtcaca aattgcaggt aacttagctg ggattgtctc ggaagaaacg
     4621 caggtcggtg tgctatctat tgtggaaaac ccgcaaaaag aaatcgaaaa gaaaaatagc
     4681 gacaggccaa ccttaattag tcgaaaagca ggagggctaa atggccaaga caccacaaca
     4741 gtattggaat gaccgtcaga agcaactgtg ggctaatttg gagacatcag aacgcgcttt
     4801 acagactaag cttagtaaat gttatgctga tgaagcaaag tccttagaaa aggaaatagg
     4861 ggcttacttt agcaagtatg gaaaaggcaa cgttattgag tatcgcaatc ttttgcaaca
     4921 gttatctaaa gctgataaag acttgcttta tcgagattgt gagcgatttg cgaaaaaata
     4981 tccacagcat gctgatttaa tgcctgtcag aacctctatt tataagttgg acagattaca
     5041 aggtttggaa ttaagcatta aaatgcagca gttggaaata ggatctattg aagaagcgga
     5101 attgaccaag catttaacta cagcctttaa aaaaggttat caagaaaccg ctaaaacaat
     5161 tggtttccaa gcggataaag tatccgcaga gctgtttatt aacaatgatt ggactggtaa
     5221 aggtaatttt agctctagta tctggactaa taaagacaag cttgtcgatt atctaacaaa
     5281 tgactttaag acggcaatta tcaggggaga tagctttgat aaagtcgtca aacagatgtc
     5341 agagcgcttt actgtacggt cacagtcgga cattacacga ttgattatga ctgagggtac
     5401 ttacgtcaac aatcaagcga tgatgaaacc tttcgaagat agcgggctat atgacgaatt
     5461 cgaatttgta gctgttttgg atggtcacac atcatcacta tgcaaaggat tagatggaca
     5521 gaaatttcca atgaattcta aacaagttgg tattaatttc ccgccaatgc acgcaaattg
     5581 tcgaagcact tttgcaatgg ttattccaga tgattatgta gacagatacg agaaggagaa
     5641 taattaatgt tttggacttt atttagtgaa ttgtgtggat tcattttgtt tttaatcgct
     5701 ttggttattg taatcgcaat tatcgtaagt acttatcagg tatttaaaga gcaattgaaa
     5761 aaataggaag aggttttttt atgaaagtat acgataaata tttaaatgaa gaaaaagaca
     5821 gattagtcga agtccatacc gagtggctga aagagattga tggttatatt aagaatcaag
     5881 aagataaaat cgaaaaatcg gaattgaaat tattagaaac tgaagaaatt tttatcgaac
     5941 gttttgaacg acttgaaagg cgaatcaaag aattggaaaa tcacgataaa ataagcttta
     6001 aaattgacga aaatgaatta gttcaaaaac ttaatgattt aacagaaagg agcatcgcac
     6061 ttgttaaagc gagagtttta aatggaataa ccggaaaaga atcttagacc tattaacagg
     6121 tctttttatt atgtcctgtc gcatgacaaa aaactaggca aataaaaatc taaagcaatg
     6181 cgctggggct tttgagcaat ggcgtggggc aggaggaaaa aatggaaagc gaagaaatcc
     6241 tagaacaatc tggtgcacaa gaagaagcta aggaacaaac gtttgacgat attttgtcag
     6301 accctaaaaa acaagctgaa tttgacaagc gtgtagctaa ggctattgat accgctaaaa
     6361 gcaaatggca agcagagacc gaagaaaaag aaaacgaagc taagagactt gcaaaaatga
     6421 atgctgaaca aaaagcgcaa catgaaaaag caaagcttga agaacgtatc gctgaacttg
     6481 aagctgagcg cacactgtca gaaatgaaga gcgctgctcg cacaatgcta tcagaagcta
     6541 atatcagcgt atctgacgca cttttatcgc aattggtatc tactgatgca gataaaacca
     6601 aaaatgcagt agaagcgttt tctgaggcat tttccgaagc agtcgaaaaa gaagttaaag
     6661 aacgccttaa atcgccaaca cctaaaaaat caaatggtaa tagtggactg acaaaagaac
     6721 aaattttagc agtcaaagac actgctgaac gacaaaaact cattgccgaa aatatcggcc
     6781 tattccaata aaacaggaga aattaacata tggtaacatc acgtacttat ccagaagaaa
     6841 atctaatcaa atcaacagac ctcaagtatc caatcactat tgatgtcaca aacaaattcc
     6901 aagaaaacat ctcgaaattg cttgaaatgc ttggagttac tcgtaaaatc tcagtatctg
     6961 agggaatgac tcttaagaca tacgctggtt atgatgtcac attagctgat ggcacagtac
     7021 cagaagggga agttattccg ttgtctaaag tcgctcgcaa aacacactct gagaaaaaaa
     7081 ttgaacttaa aaaataccgc aaagcgacaa ctggcgaaga tattcaaatg tttggttcta
     7141 acgaagctgt aacaaatacc gacaacgctc ttgtccgtca attgcaaaag aaaattcgta
     7201 cagactttgt gaccgctctt aaaacaggta caggcgaaca aacagctctt ggagcaggct
     7261 tgcaaggcgc cttagcttct gcttggggta aattgcaagt gctctttgaa gactatggtt
     7321 cagagcgtgc aatcgtcttt gccaatagcc ttgatgtcgc tgaatacatc gctaaagctg
     7381 gcatcactac tcaaaccgca ttcggattga cttaccttgt cgattttaca ggtacagtca
     7441 ttatctcaac aaacgatgtt gcaaaagggg aaatctgggc gacagtacca gaaaatatca
     7501 tctttgctta catcaatcca aataattctg aattagctaa agaatttaat ctttacggtg
     7561 atccaaccgg ctacattgga atgaatcact tccaagaaaa tacaacgctt actatccaaa
     7621 cgctgcttgt atctggtatg ttgatgtatc cagaacgcat tgacggaatt gttaaagtaa
     7681 ctcttacacc aggcatctaa ggggggaatc tatggcttac acagtaaaag cacgtttttt
     7741 tgacttgctt gacaatagct ttctttacga ggttggagac atctttcctc gtaaaggcta
     7801 tgagccatca aaggaacgat tagaaagttt actatcatct aataacgctt atcacaaacc
     7861 ttttatttca gcatctgaaa gcttgcacga tatgaaggta actgaattaa aagaaaaagc
     7921 caaagattta ggcattgaat taccagcaaa agcaaataag gaagaaatta ttgaattaat
     7981 tgaaaagaaa tgaggtgact tatgagtgct aatgaagata ttctagctcg tgtaaaaata
     8041 aggttatctg acgaagcaaa tgttaatcca ttacttcttt ctgaattagt tcaaaccgct
     8101 attgaccgta tcaacttaaa agtaggggat gttattttta atcctatttt taactctatt
     8161 gctgtggacg ttgtagttaa aatgtatcgc agattatact acgagggaat ctcaagcgaa
     8221 gatgctgaca caattagaac aacatttgtt aatgacttat tggcagaata tggcgaagag
     8281 ttggcgagtt acaaaaaaga ccgtttagca atactaaata aaaaggtggt acgtttctta
     8341 tgagatatac cacttttaat ttagttacag atgtcaaaac aggcaaaaaa gatgctttgg
     8401 gcaatgacat cataactaaa acagaatcta taagtcacaa agggaggttc acagagtggc
     8461 acgcagatga tgagattgta tatggtcgag atttaacgtc tagcactcgt aaaatgataa
     8521 gtaatacaat aacacttgga caagctaaaa aagcatctca ggttaaatta gacggcaaat
     8581 attacgatat aaaatcagtt aaagatttag gtcgttggcg tttgttattg attaatggtt
     8641 atcgcttatg aaatttacaa ttaaaggcac tgaaaagctt gaagaacgtt ttagagcgat
     8701 gaatgctatt cggtgggacg gtgttgtcaa taaaagtctt actcaaatgt ttaatcgagg
     8761 tgctagaccg ccaggcacac cagttggaaa acaaaccaaa aatcatagcg ctggggaatt
     8821 aagacgttca agaagagtta aaaaaatttc atctaaagat ggttcttata caggttcttt
     8881 ttactataca aaggattatg cacctcacgt tgagtatggg cataggatag ttagaaaagg
     8941 caagcaagtt ggttatgcca acggtaaaaa atatcttttt aataacgttc aaagacaacg
     9001 tcaaatttat tttaaagata tgttagctga attaaggaag aagaggtaaa gcatgttaaa
     9061 aaaattaggt ttgacagatt tacacgcctc tttaaaggaa acaattgaaa aaaatacagg
     9121 tgttgtagcg tatgattctg ttccagaaga tgcgccttcg cctttttatt ttattgagat
     9181 tgtggataaa agaccagaag acacaaaagt catgtggtgt gaagtattca ctatttggat
     9241 tcatgcaatt gcagaaaaag gtaagtctaa aatagcaata tataaaatga ttgaagattt
     9301 agaagaagct ttaacaatgg agttagcatt gccagatggt gttgaagtgc tacgacaagt
     9361 agaaactggg atgcagtcct tacaagagga tgaaacgggt gagaatcatg cgattgttgc
     9421 ctatgaaata aaagtcgctt atggctttaa aacaaagata taaaaggaga aaataatggg
     9481 aacagaattt gacagtggtg tgtattgtaa ttttgatgca agctccacaa aagcaatcgc
     9541 tggtaaagat attatcttgg caatttttga tgcaactggg gctaaattat tggcaatcag
     9601 tgggcaacaa gggttgacga ttaatcgttc agctgactcg attgaagtgt catctaaaga
     9661 cactaaaggt ggatggaaat ctaaaatcgc tggtatgaaa gaatggtcta ttgacaacga
     9721 tggtctatat attccttcgg acgaaacaca caaagaacta ggaaaagcat ttgaagcaag
     9781 taacccagtt tgtatcaaag tagtaaacgg taaaactaaa aaaggtatgt ttggcggatt
     9841 agcgtatatc agtgattact cactagaggc gccatatgat gacggtatga cttactcaat
     9901 cagtttagac ggtaacggtg cattagttga cttatcaaca ttgacagaac aagctgattt
     9961 agtagtagct atgcctgaat aatagagagt aggaaaaatg gaaaaagaaa ttatcaaagc
    10021 agatgaaaag cagtatgaat taaaatataa cgaaaaaacc attgaaaccg tcgaagcttt
    10081 gacaggtaaa gcgtttatgg atgttattgt gaataacaaa ggtatgttgt cgctatctat
    10141 gttgcgtcag tactttgcta acgctcttta tgcggtagaa ggcggtcgtg tgtctagcga
    10201 gcaaggctct aatattttta ctaaagtgtt aaatacaaaa ggttatgcgt atgtaaatat
    10261 gctagtgatc aacacaatcc agcgtgattg cccttttttc ttcctcggcg cttagtcgac
    10321 tttgagtatc tcggcgggtc tggcgatagc acagatgtag agtatgacag attagcaagt
    10381 cagtatcaca aagatattga ctttgctttt tattttgtca attttgggac tacaaaatcc
    10441 gaatttttgg aattaaccag acgagaaaaa gcctttatca ggaaagcttg ggaagataaa
    10501 caagtgcgag aaagcgagct tatgcgcaat gctgtgctaa atgcagtcag taatgctatg
    10561 cgcaaaaaaa gcgctaaatt tgtggatttg tggaagcgtc agcaacagcc tgcgaacatg
    10621 gaaatcgtta acgctcactt agaaattgta tctatatctg aagaaactga aggtaagtcg
    10681 tgggttgatg caatttacaa agctaacaac ttaaaaacac caaaggggga ggaggtaggc
    10741 aatggctgac tatactttag gtgtacaagt aacaggtgac gcttcgagca tgcaaaagga
    10801 atttgataaa gctcaaaaag cagttgaagc tttaaagaaa aaaaccgatg aagcaaataa
    10861 aacagcttca ggaactttca gcgaaatgtc aaaaagtata ggagatacct cagaaaagtt
    10921 aagaagtttt ggcgataaaa tgagtgaagc cggtaaaaaa atagcagatg tcggttctaa
    10981 catgactaaa tggataacaa ctccagttgt tggagcggtt ggttacgcag tcaaagctta
    11041 cgcagatttg gaacaagccg tgggcggtgt tgaaacacta ttcaaaagct catcaaaaga
    11101 agtaatctca aatgctgaaa aagcttataa aacagcgggt atatcatctg ttaaatacat
    11161 ggaacaagtg acgtcatttt cagcaagcct attacaagga cttggcggtg atactgctaa
    11221 ggctgccaag gtggcagata aagctattat cgatatgtct gacaatgcta acaagatggg
    11281 tacatctatt gagttaatcc aaaatgctta tcaagggttc gcaaaaggca acttcactat
    11341 gttggacaac ctcaaacttg gttttggtgg gacaaaagaa gagatgcaac gattacttga
    11401 agaagctgaa aaagtaactg gtattaaata cgatattaat aacttgtcag atattattga
    11461 ggcaatccat gtaatccaaa ctgaactagg tattactgga actactgcaa aagaagcaga
    11521 atcaactata agtgggtctt ttaaccagat gttgtcatca ttaaacaact tatcagctgg
    11581 tcttggagat aaaaacgcag acatcaagaa attaatgaat gaactggaac aatcaaccag
    11641 aacatttgtc atcaatgtat ctaatgtatt aaagacaatc tgggataatt taccacttga
    11701 gccatggcaa aaatggacta tcgcagtagt tgcattagct ggtcctgtat tattgatttt
    11761 tggaaagtta actgttggga tcggtaattt gataactgcc tttgggacaa taggagaagc
    11821 tatttctagc ataattggat ggtttggaac gctaacagca gaaggtggcg cattagcagg
    11881 tgtgtttggt tctcttggag ccggacctat tattgcaatc tttgcaggga tagcacttgc
    11941 gattggatta gtagtattgg ctataaaaga tttatgggat aattccgaag attttagaac
    12001 tgctgtaaca gaaatatggg caagcatttc aacgatgttg acaaatgtct gggatagctt
    12061 tctattacct gtttttgaag caataaaagc tattatattg tcgatagttg atgaatcgtt
    12121 aatccctcta tatgacagtt ttaaacaatc attagaagac atagctattg aattgtcaac
    12181 ctttttaaaa atagcagaac cgtttatctc tacacttgct gacctttttg ggaaaattat
    12241 ccctccaatt cttgaaattt taggtgctaa ttttaaatgt acgtttgcta ttatttcgaa
    12301 tgtcatgact gttgcattcc aaacgattag tggaatagtt aagaatcttc ttaaagtatt
    12361 agggggaata ataaccttta tcactggtgt gtttactggt aactggcgca aagcttggca
    12421 gggtgttgtc aacatgcttg gcggaatatt tggtggtatt ggagtaatca tgaaaggtgc
    12481 tattaatggt gtcattgggg ttattaacgg cgcaattggc ggaatcaaca gcttgatagg
    12541 cactgttaac aaaataccag gggttgatat tggcactatc ggctcaatcc cttatcttgc
    12601 tagaggtact gacaactggc aaggtggatt tgctcggatt aatgaaggtg gtcgaggaga
    12661 attggcgtac atgccaagcg gtgcagtagt agttccacat gatgtcagca tgaaatatgc
    12721 gaaagaaagc gcaagagcgc aatcaggcat gattatggtt agcggaaatg atgaaatagc
    12781 taaaaatgcc cttaaattag ctaacgaggc tgtcaaaaga cctgttatat tacgagtaaa
    12841 tggtagagat ttagcagtaa caacaggtaa agattttgct aattatcaag agagacaaac
    12901 agtaacgtta aaaagaatga gaggtgagtt ataatttggc aacgatgaca tttaatggtg
    12961 ttgatttatc aggtttaatc aatatcatga gtattaatag ggatatcgga aatgaagtta
    13021 ccttaacaac tgatgactca cctaattttg gttcacatgt tcaaaagtca aaaattcatc
    13081 caaaagtaat ttctgttgat ttttatataa aagacaaatc ttctgactat gcacttaatc
    13141 agctcaaaca tagattggct ggtgttttta atgttaacaa agatgtcagg ataacattta
    13201 gcgacgagcc cgacaaatat tatttaggca gaccgattaa taaaatatcg gcaggagacc
    13261 caatagcttg gttaagctta gtcacattag agttgtttat cccagatggc gtcgcgcatt
    13321 caactactta caaaaagttt ttagattaca cgcaagatgg aaataaacta acctttaaat
    13381 tacaaaacga gggtaacacc aatgcgctgc caattatcaa aataaaacac aactccgaaa
    13441 atggctatat cggcatcgca aacgaaacag gtgcttttgc gcttggatca tcagaagaag
    13501 aagacgggac tatcgtgcat cgtaacgaaa ccctttttga ttactcaaaa gcgatagcac
    13561 aagctttgga cggtgcgcca aacgtcgcaa aacttaatca catgccaccg acgtacgata
    13621 cagagctgaa acgcaagcgc attgataata ttttaggttc tggcaaaggt ggtgaatatg
    13681 ttgttattgg aaatagaggc accacaccgg gatacacaga acatgttggg actcgaacgt
    13741 ttattatcaa tcctgattca aacggagaat acactctcaa tgagcacctg tggtggcaac
    13801 agatttttat tgctactgcg caggatcaga aaggtttttt aaagctttgt gtaacgggaa
    13861 tcgacgatga aggaaatgac gagtttttgt atggaatcga aacttacaaa cggaaaaatg
    13921 gttttgaaac agaatacaat ttttttgcgc ttgatgacga cggtgtgggt tggagatttt
    13981 ataagcagtt taaattccaa gcagacagaa attatcacaa tcctttttca atgaatagaa
    14041 gtagagcggt tgagattttt agagaagaag ataagtttcg tatttacttt aacggtgcgc
    14101 atcatcatgt aactgttccg tcccttaaag gaaaaaaatc ccgcaagata catcttgcaa
    14161 tggggacatg tagtgatagc tctaaatata tcaactacaa cctgtttgaa aaagttaatt
    14221 ttgaaaaaat gggagtgtct cattacaaca atatcgtcaa taaatatcaa ccaagcgacg
    14281 aagttgttat taattttgaa aatgatacag tcaaaactaa agaacttaac tccctgcaag
    14341 acatggtctt aggctctcaa ccaatatcta taccaccagg agaatcagaa ttggttatcc
    14401 agttatctaa attttctcag tctgcaccaa atgttgaaat tcttatggaa gagaggtggt
    14461 tgtaataact ctagtaatac acgacgcaaa gttacatcca gttttgcttt tagacaatga
    14521 taaacaagga gcacttaatt attatgatga tttgtggact agacagctca caaatggttc
    14581 atcagtcttt gagttttctg tttataaaaa aacgcttgaa ggagataatc cactcaatca
    14641 taagtatcaa gtgcttaatg accaagcgtt cgtatcgttt gtacataatg ataaagtaca
    14701 attgtttaac atcatgcgag tcgaggaaac agagacaaca atac
//
LOCUS       DATRQA010000042        14704 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.042, whole genome shotgun sequence.
ACCESSION   DATRQA010000042 DATRQA010000000
VERSION     DATRQA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14704
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.042"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            110..1525
                     /locus_tag="VBD27_000945"
     CDS             110..1525
                     /locus_tag="VBD27_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7024971.1"
                     /translation="MKFFEENYSQEIPTRIKNLRKKYNITQSELGNAGQVSQVESGKR
                     PITSSMLVYLNALTASSYTYIVFGELDEFIENLFHYFFSSILYRDLEAVDEKLYSFMS
                     DDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKDDIDVWVGGKSYNPARS
                     FRTRTINELTVIDFEEMFDILWLMLGDNLIKSFEVNVCGILFELGGNDIPSTFRQENI
                     DPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFMVNDILERMYKENIPKSYLTSVPL
                     VISQKGRTTYSFSMTGGQQIDGVKFTQIYEDYMKLLSQGKDIAELYQKYSKEELANLG
                     INIYQSNDIERTEERTFDEIISWVSNPYATRPIQERHTIQLEPTRFSLEDKKRIEEAA
                     AQGLSEIDLIDLVDLYDINLDNTSVNRHIVGLLTNNTQVTYYFQEQLNKELLSMAHAL
                     DNVQQAFIKLLSEEEIRKFAL"
     gene            1845..2294
                     /locus_tag="VBD27_000946"
     CDS             1845..2294
                     /locus_tag="VBD27_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7024972.1"
                     /translation="MSNKVQERRERKIKEAIKAKNWDEVTRLLQQEQSNAERRDRYHN
                     RRIKDETIASKNAKKSVRYDVIASSDLNPEEALILEELRQAIREAKASLSEIDSKIVE
                     MIAEQGSSYKETARYITEHYKKMSDVTVKSHYCKALKKLAPLLKAYR"
     gene            2496..2816
                     /locus_tag="VBD27_000947"
     CDS             2496..2816
                     /locus_tag="VBD27_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024973.1"
                     /translation="MAFKMKTTKGGYTTTLADKIISQKQPIYSLSTELEPQQRFEEGK
                     PTGEIVAYKAWFVQEGQDPFQVKFEETIELPAFQSMIQFDTLQACEVKYNIYFKANGI
                     KEVR"
     gene            2816..3916
                     /locus_tag="VBD27_000948"
     CDS             2816..3916
                     /locus_tag="VBD27_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7024974.1"
                     /translation="MTSQSRDVLNQSYLQSYLLQSLNMALGALMQGETSYTNSFNIVI
                     QADGFIFVPRLPCAYILDDDLYKKIFLIANASLYPQYTLLKQNATYFVPLETDDLHTQ
                     RGLFFPWKKGISERLVIPDLDKFSASLPQGKIPIMKYFELNLDKVNHWAIAGNSGSGK
                     SYALTYFLSVLKHMSDLIIIDPKFDTPSRWARENHVAVIHPVENRSKSDFVSQVNEQL
                     SQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQISLLG
                     RATKIHLFLVSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPIG
                     HGTGIIQVIDNEHPYQVLPLLCPTYYTKRGIL"
     gene            3913..4518
                     /locus_tag="VBD27_000949"
     CDS             3913..4518
                     /locus_tag="VBD27_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7024975.1"
                     /translation="MTNKTYFMRSFNLILYLFLFAPICSAFGWLFNYLSVQLTNITFV
                     NLDTVKSITDIISSVCHGFALISLLLALFLVGLEIIQRLKTDSLWNYIQSVYHTFLLR
                     HFLFQRERVQKISNLEHQTVTTINPIHNGFNRAVRKCIVDIRKDTITIFLKVPRDQQG
                     QKILKEMEAQLKEEIVSQHADYYFSSPIRVRNQLWFTGQKR"
     gene            4724..5329
                     /locus_tag="VBD27_000950"
     CDS             4724..5329
                     /locus_tag="VBD27_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO7024976.1"
                     /translation="MAKEQRSTKWTFLFYEESAPENYLNILEELHIPFILSPWHDKDV
                     NRQTGEFKKSHKHGAFFFDSLKSYSQVSNIISDKLNGPAHVEVVMSPTGLFDYFTHAE
                     NPDKTPYNIEDIEVGCGFNLEKFLMEMNSSDFIHEVVDIIEENDFTEFEELVWYARAN
                     NTNLLGLIIERTYFFAKYLDSRRYNPNRLHNSNTEEKENNE"
     gene            5322..5570
                     /locus_tag="VBD27_000951"
     CDS             5322..5570
                     /locus_tag="VBD27_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7024977.1"
                     /translation="MNNEKLEQIEQLLQTLIKLIQIKEQNIPLKIIQQRELLKQLNIS
                     PNTLKTWEQKGLKRLEPPIEGTRTVFYLLDDIINFLQS"
     gene            5660..6925
                     /locus_tag="VBD27_000952"
     CDS             5660..6925
                     /locus_tag="VBD27_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7024978.1"
                     /translation="METETIIHNGKKVIKKIKKDKSISYTLKGTFLGKDIKTGKQVTT
                     TITAKTLKQLDRAIIQARLEFEKNGSTREKVIVIDTLEDLAEEWFKSYKTWVNSHNTL
                     NRVRGYLDNYIIPKFGDYKPDKIESADIQLWLNDLAKKSKESIESGIKRADKGSAKDF
                     GAVIHKLKDIFDYGITNYGLITNPVSTVKIPPKPKSNKQRIMVLHDDGLVIWLNYLES
                     LDNNRANRRFKLICNTLLASALRINELLALTIDDLDFENSSINVSKTLMWKTANKKDG
                     TKGEVICKATPKTDAGNRSVPVPPPIIEKLRDFHHEMNKYLELHNRHRTKLIFPTIYG
                     NYMCDRNERTTLKKRLVGLGLPNYGFHLFRHTHASMLLNAGTNWKELQVRMGHKSIST
                     TMDTYAELAPQRKLEAVGIYLEKIAELTQ"
     gene            7284..7955
                     /locus_tag="VBD27_000953"
     CDS             7284..7955
                     /locus_tag="VBD27_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO7024979.1"
                     /translation="MTNYKNNFKKFMSNKLYVGLASITLLGIGGAIGVSGAALAHNHH
                     SEHMSFEHKHEGHDKHHDHREKYSLKTLKNISTKIDYKTALETAKKEARSGFISQIEL
                     NTKGDTPFYEIKVIDNKIAKKYKIDANSGKILSTTSDNQLNSKDAQLKQPSKDIQTAL
                     QAIQAKYKNAKIKEISLANNKSNKLVYKVKLLDGNTPKIAQFDSDKASFIKANKLNTT
                     HKNHD"
     gene            8040..8711
                     /locus_tag="VBD27_000954"
     CDS             8040..8711
                     /locus_tag="VBD27_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7024980.1"
                     /translation="MKILVVEDEFDLNRSIVKLLKKQHYSVDSASNGEEALQFVSVAE
                     YDVIILDVMMPKMDGFTFLKLLRNKGSQVSILMLTARDAVEDRIAGLDFGADDYLVKP
                     FEFGELMARIRAMLRRTNRQVSSDDIQIQDITINLSTKQVWRNDNLIDLTAKEYEVLE
                     YLARHRDQVLSRHQIREHVWDYDYYGESNIIDVLIKNLRRKLDNNRDGSLIKTKRGLG
                     YVIPK"
     gene            8695..10059
                     /locus_tag="VBD27_000955"
     CDS             8695..10059
                     /locus_tag="VBD27_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7024981.1"
                     /translation="MLFQSKINYPKKSSIITQITLWYSSFIFILVIGVLIGSFFISKS
                     IAENKSKKNLEAKAVQMSQALAKGHRYEAFEDGIFYSVYDQNGKVIYSGFPKGFKRDL
                     DHQHKHKKKLSLFSMENRTFQYVDIPISGKNQWLRAIRTVDRLDKQLTELLFSLGIVL
                     PLMLIIITVGGYLILKRAFRPIQEITETAQFITQNEDYTKRIITKNNENELTELAAVI
                     NTMLASIESSFVREKQFNNDVSHELRTPVTVILSESEYGKNYAENLSEAKESFEVIHR
                     QSLSMKKLVEQLLELTKAENPLSIQLEPLNFSIMMKQLVSDSSRLLDNTPIHLDSQIE
                     DDLWIIGQQTLLKRLFDNLFSNAIKFTNNHISISLRQSDNQIVFSIKDNGLGISVDDQ
                     SKIWNRFYQVDSARTKDSQSGIGLGLSLVKQIATIHRAKIWVDSKPDDGSQFTLTFPK
                     LKKD"
     gene            complement(10169..11722)
                     /locus_tag="VBD27_000956"
     CDS             complement(10169..11722)
                     /locus_tag="VBD27_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEO7024982.1"
                     /translation="MPEKMTNSENKRSKGFRMPGAFTILFILTIFSVLATWWIPAGSY
                     SKLQFDTASSKLVVTDPNGKTVHVPATQTQLDKMNVKIKIKEFTSGAISKPVSVPNTY
                     KRLKQNPAGIGSVTTSMVNGTIEAVDIMVFIMVLGGMIGVVRKSGAFESGLLALTKKT
                     KGREFLLIFLVSLLMVLGGTLCGIEEEAVAFYPILVPIFLAMGYDSIICVGAIFLASS
                     VGTSFSTINPFSSVIASNAAGISFTEGLSWRTAGCIAGAIFVVVYLHWYAKKIKANPE
                     FSYSYEDRVEFNAKWGMTTNHTPSLFTIRQKIILSLFVISFPLMVWGVMSQGWWFPTM
                     ASSFLAITIIIMFLTATGANGIGERDVVDEFVNGASSLVGVSLIIGLARGINIILSQG
                     YISDTMLYTASKLASHVSGPVFIIVMMFIYFVLGFVVPSSSGLAVLSMPILAPLADTV
                     GIPRSVVVMAYQFGQYAMLFLAPTGLVMATLQMLDMKYSHWLKFVWPVVLFLLIFGGG
                     LLVLQVLIL"
     gene            complement(11816..12742)
                     /gene="arcC"
                     /locus_tag="VBD27_000957"
     CDS             complement(11816..12742)
                     /gene="arcC"
                     /locus_tag="VBD27_000957"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO7024983.1"
                     /translation="MAKIVVALGGNALGNSPEEQLRLVKHTAKSLVALIKKGHEIVVS
                     HGNGPQVGAINLGMNFAAESGQGTNFPFPECGAMSQGYIGYHLQQSLLNELRQEGINK
                     EVATIITQIEVDESDQAFSAPTKPIGTFYDKETSEKIAIEKGYTFVEDAGRGYRRVVA
                     SPEPKKIIEINSIKTLIENDTLVIAGGGGGIPVINKGGYEGIAAVIDKDKSSALLAGE
                     LAADQLIILTAVDYVYTQFGKENQKALTEVNENQMIDYVNQGEFAKGSMLPKVIACMS
                     FLDHNPKGIALITSLNGLEDALDGKLGTRITK"
     gene            complement(12754..13752)
                     /gene="argF"
                     /locus_tag="VBD27_000958"
     CDS             complement(12754..13752)
                     /gene="argF"
                     /locus_tag="VBD27_000958"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO7024984.1"
                     /translation="MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLV
                     GKNIALIFEKDSTRTRCAFEVAAHDQGAHVTYLGPTGSQMGKKETSKDTARVLGGMYD
                     GIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLADFLTAKECLHKPYKDIRFTY
                     VGDGRNNVANALMIGASIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGASIEITADI
                     AEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPS
                     FHNIDTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD"
     gene            13855..>14704
                     /locus_tag="VBD27_000959"
     CDS             13855..>14704
                     /locus_tag="VBD27_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7024985.1"
                     /translation="MLDILSEKHLERRQRIIISAIAIALAAQINISILADGFIMTLSL
                     FILPVFLYFNDDINPILLCLGITFASPIFRGIILSIAGEAEIHQIIEFVLTDMAFYIC
                     YGITFYTIYWHRSYRNKGTFFFSIIICDYFANLVEISFLIKFNNYTITIFATLFAIAL
                     LRAFISCAVAYTYSYLSLLLQKDDHERRYYYFMWSTSAVKSEVYFMQKNIIEIENIMK
                     NAYLLDKELSKYHLPKEYQHLSLDISRDVHEVKKDYQNIIKGLGTYFSVKNESTMALK
                     DIFQIVL"
BASE COUNT         5116 a         2700 c         2382 g         4506 t
ORIGIN      
        1 ttatatatta ttttataaaa gatgaggaaa tcagagattt ttttaatcaa ctaggataac
       61 aactatttct actactttct acttttccat gattataggg ggattatata tgaaattttt
      121 tgaagaaaat tattcacaag aaatacctac tcgtatcaaa aatttaagaa aaaaatacaa
      181 tattacacaa agcgaactag gcaatgcagg tcaagttagt caagttgaaa gtggtaagcg
      241 accaattacg agttcgatgt tagtttattt aaatgctcta accgcttcga gctatacgta
      301 tatcgttttt ggagagttag atgagtttat tgagaatctg tttcattatt ttttcagttc
      361 tattttatat agagatttag aggctgtaga tgaaaaacta tattctttca tgagtgatga
      421 tttaatttca atccaatcaa gttgtctaag tattgccaag acatttgcaa atttcaatat
      481 tcaaagaaaa agatttatga tttctactga aactgaaatg gatacttttc acaagaaaga
      541 tgacattgac gtatgggttg gtggaaaaag ttataacccc gctagaagtt ttagaacccg
      601 taccatcaat gaattgactg tcattgattt tgaagaaatg tttgatattc tttggttgat
      661 gttaggggat aatttaatta aatcatttga agttaatgtc tgtggtatcc tatttgaatt
      721 aggcggaaat gacataccct caacatttag gcaagaaaac attgatcctc tcatcaataa
      781 atggtggtat gataatgttt cgacagaaat tatccctaat ctaataaaaa aactcaaaga
      841 aaatcccttg tttaatatcg gatttatggt aaatgatata ttagaaagaa tgtataaaga
      901 gaatattcca aaatcttacc ttacatccgt cccattagtt atttctcaaa aaggaagaac
      961 aacctattcg tttagtatga ctggtggtca acaaatagat ggagtgaaat tcacacagat
     1021 atatgaggac tatatgaaat tactcagtca aggtaaggat atcgcagagt tatatcaaaa
     1081 atattctaaa gaagagttgg caaatctagg cattaatatt tatcaatcca atgatataga
     1141 aaggactgag gaaagaactt ttgatgaaat tatcagttgg gtttccaacc cttatgcaac
     1201 aagaccaatt caagaaaggc acactattca attagagcca acaagatttt cactagagga
     1261 taagaaaaga attgaagaag ctgcagctca aggactaagc gaaatcgacc ttattgattt
     1321 agttgaccta tatgatatta atttagacaa tacaagcgtc aatcgccata ttgtggggtt
     1381 attgactaat aacacccaag taacatacta tttccaagaa caattaaata aggagttgct
     1441 gtcaatggct cacgctttag ataacgtaca acaggccttt attaaattat taagtgaaga
     1501 ggagatacga aaatttgctc tttaaagact aggtgaatcg cctagtcttt tttgcgcctc
     1561 gttttacaaa ttagtctatt gaagtaaaaa aagaagtaaa aaataaaaga acattacgcc
     1621 ttatactgaa tatatcaaga aaaactcttg atataaattt taatagtcca aattgatggc
     1681 catcaaagga cagattgaaa caaatatgat gacatgacga taaaggtcat gtcttgcata
     1741 gaattgtttc gctgtttctt tgttgtgcct tttggtatac tcagcgttca atatttgctc
     1801 attttgtttc cttctgtcca aaggacagaa aggagctcat cattatgagt aataaagtac
     1861 aagaacgtcg tgagcgtaaa atcaaagaag ctattaaagc taaaaattgg gatgaagtaa
     1921 ctcgtttgct tcaacaagaa caaagtaacg cagaacgtcg tgaccgatac cacaatagac
     1981 gaatcaaaga cgaaacaata gctagcaaaa atgccaaaaa gtctgtacgt tatgatgtca
     2041 ttgcaagttc tgatttgaat cccgaagaag ctttaatcct agaggagtta agacaagcta
     2101 ttcgtgaagc taaagcatcc ctatcagaaa ttgacagtaa gattgttgaa atgatagcgg
     2161 aacaaggttc aagctataaa gaaacggctc gctatatcac tgaacattat aagaaaatga
     2221 gtgatgtaac tgttaaatcc cattattgta aagcgctaaa aaaattagct cctttattaa
     2281 aagcttaccg ctaatccctc aaatccagtg tctctgtcac tggatttttt gtaaaaaaaa
     2341 attaaaaatt caacaaataa ctttgcgttt agctgtctca ttctccttag taatgaggag
     2401 gtggtcagat aacctaaaaa acaaagtcca gctcatgacc caaaccgagc ctgtcgcatt
     2461 cacactatct cttattcata aggaggattt gccctatggc gtttaaaatg aaaaccacaa
     2521 aaggtggtta tacaactaca ttggctgata aaattatcag tcaaaaacaa ccgatttact
     2581 cacttagtac cgaacttgaa ccacaacaac ggtttgaaga aggaaaacca accggagaaa
     2641 tcgtagctta taaagcatgg tttgtacagg aagggcaaga ccctttccaa gtaaaatttg
     2701 aagaaacgat tgagcttcca gcttttcaat ccatgattca atttgacaca ctacaagcct
     2761 gcgaagtaaa atataacatc tacttcaaag caaatggtat caaggaggtt cggtaatgac
     2821 ttcacaatct cgtgatgtgc tgaaccaatc ttacctgcaa tcttacctgc tccagagcct
     2881 aaacatggct ctgggggctt taatgcaggg agaaaccagc tacaccaatt cttttaatat
     2941 tgttattcaa gcagacggct ttatctttgt ccctcgtcta ccatgtgctt atatccttga
     3001 tgatgacttg tacaagaaaa tctttctgat tgccaatgct tcactttatc cacaatatac
     3061 gcttctaaaa caaaacgcta cctattttgt ccctctagaa acagatgact tacacactca
     3121 gcgtggatta tttttccctt ggaaaaaggg aatttcagaa cgtttagtca ttccagattt
     3181 ggataagttt tcagctagct tgccacaggg gaaaatccct atcatgaagt actttgaact
     3241 caatttagat aaggttaatc actgggctat tgctggaaat tcaggttctg ggaaatcgta
     3301 cgctctcact tatttcttga gtgtactaaa gcatatgtct gacttgatta tcattgaccc
     3361 taaatttgat accccaagtc gatgggcaag agaaaatcac gttgctgtta tacaccctgt
     3421 tgaaaaccgt tcaaaatcag attttgtctc acaggtcaat gaacaattaa gtcagtgtgc
     3481 aactctcatt cagaaacgac aagctatctt gtatgataat cccaatcatc aatttaccca
     3541 tctaactatt gttattgatg aagtcctcgc tctatcagag ggagtcaata agaatatcaa
     3601 agaagccttt ttctccctac tctcacagat ttccttatta ggacgtgcta ccaaaatcca
     3661 tctgtttttg gtaagccagc gttttgacca caatactatt ccaatttcag tgagggaaca
     3721 gctaaacgta ttgttgcaaa ttggaaatat caatcagaaa actactcaat ttttatttcc
     3781 agacctagac ccagagggta ttgttattcc aataggacat gggacaggta tcattcaagt
     3841 tatcgataat gaacatccct accaagttct gcccttactc tgtccaacct actacactaa
     3901 acgaggtatt ctatgaccaa taaaacctac tttatgagaa gttttaatct catactatat
     3961 ttatttctat ttgcacctat ctgttctgca tttggatggt tattcaatta tctttccgtt
     4021 caattaacaa atatcacgtt tgtcaattta gatacggtca aaagtattac ggatatcatt
     4081 agttccgttt gtcatggatt tgcacttatt tctcttctac ttgccttatt tttggttgga
     4141 ttagaaataa tacaacggtt gaaaacagat agtctttgga actatatcca atctgtctat
     4201 cacacttttc tgttgcgaca cttcctgttt cagcgtgaac gagtacagaa gataagcaat
     4261 cttgaacatc agacggttac tactatcaac ccaatccata acggatttaa ccgagctgtt
     4321 cgtaagtgta tcgtagatat acgaaaagat accatcacta tttttctaaa agtccctaga
     4381 gaccaacaag ggcaaaaaat cctaaaagag atggaagcac aattaaaaga agaaatcgtc
     4441 agccaacacg cagactatta tttctcctct cctattcgag ttcgtaacca attgtggttc
     4501 actggacaaa aacgttaatc ccttggggct gtggcttgcg ttaagcaaaa gccagacagc
     4561 ccctttttat attcttttct atcatgacct attggggtga ctacgacccc caataggtca
     4621 ataatcaata atcacaaaaa attcaatttt ttcaaaaaaa ctttgcatac gacctgtcat
     4681 ttctccttag taatagaacc caaaagaaat gaggtaactg cctatggcaa aagaacagcg
     4741 ctcaaccaaa tggacttttc tcttctacga ggaaagcgcc ccagagaact acttgaatat
     4801 attagaggaa ctccatattc cgtttattct tagtccttgg cacgataagg acgttaatag
     4861 acagacggga gagttcaaga aatcgcacaa acacggtgct ttcttctttg attcactgaa
     4921 aagctattca caagtatcaa atatcatcag tgacaaacta aacggtccag cacatgtgga
     4981 ggtagttatg tctcccactg gtttatttga ctactttacc catgcggaaa atccagataa
     5041 gaccccctac aacattgagg atattgaagt cggttgcggc tttaatttag aaaaattctt
     5101 gatggagatg aactcctcgg attttataca tgaagtagtt gatattatcg aagagaacga
     5161 ctttaccgaa tttgaagaat tggtctggta tgcccgagca aacaatacca atttattagg
     5221 gcttatcatt gaacgtacat atttcttcgc taaataccta gattcacgaa gatacaatcc
     5281 taatcgactc cataattcaa acacagagga gaaagagaat aatgaataac gaaaaattag
     5341 aacaaattga acaattattg caaacactta tcaaactcat tcaaatcaaa gaacagaaca
     5401 taccactaaa gataatacaa cagcgagaat tgctaaaaca gctaaatatc tccccaaaca
     5461 cactaaaaac ttgggaacaa aaaggattaa aaagattaga acctcctata gagggaactc
     5521 gaaccgtctt ctatctacta gatgatatta tcaacttttt acaatcctaa ttctgaaaat
     5581 ttgtgctaaa atagttttat ctaaaagaaa gagtggtaac tattttagcc tacttcaata
     5641 tccatgaaga aaggcaaata tggaaactga aacaattatt cataatggca aaaaagtcat
     5701 taaaaaaatc aaaaaagata agtccatctc atacactttg aaggggacat ttctgggaaa
     5761 agatatcaaa actggtaagc aagtcactac taccattaca gccaagacac taaaacagtt
     5821 agatagagca atcattcaag cacgactaga atttgaaaaa aatggctcaa cacgagaaaa
     5881 agttatcgtc attgatacac tggaagattt agctgaagaa tggtttaaat catataaaac
     5941 ctgggttaac tcgcataata ctctgaacag agtaagaggt tatcttgata attatattat
     6001 ccctaaattt ggagattaca aaccagataa aattgaatct gcggatatcc agctttggtt
     6061 aaacgatttg gcaaagaaat caaaagagtc cattgaatct ggtataaagc gtgctgataa
     6121 aggtagtgct aaagattttg gtgctgttat ccataagttg aaagatattt ttgactatgg
     6181 aattacaaac tatggactaa tcactaatcc tgttagcact gttaagatac cacctaagcc
     6241 aaaatcaaat aagcaacgca tcatggtatt acatgatgat ggtcttgtaa tatggcttaa
     6301 ttatcttgaa agtttagata acaatagagc caatcgtaga tttaagttaa tttgtaatac
     6361 tctattagcc tccgctttgc gaataaatga attacttgca ttgacgattg atgacctaga
     6421 ttttgaaaat tcttctatta acgtcagtaa aacattgatg tggaaaaccg caaataaaaa
     6481 agatgggaca aaaggggaag ttatctgtaa agctacacca aaaactgatg caggaaatcg
     6541 ctctgtccct gtccctcccc ccataataga aaaactcaga gatttccacc acgaaatgaa
     6601 caagtatctt gaattgcaca acagacatag aacaaaactg attttcccta caatctatgg
     6661 gaactatatg tgtgacagaa acgagagaac aactcttaag aaaaggttag taggtcttgg
     6721 attaccaaac tatggtttcc atttattccg acatactcat gcttcaatgc tacttaatgc
     6781 tggaacaaat tggaaagaac tccaagtcag aatgggacat aaatccattt caactacaat
     6841 ggatacttac gctgaattag caccacaacg aaagttagaa gctgtgggga tttatcttga
     6901 aaagattgct gagctaacgc aataactact ctaccattca ctctaccttt tattatgtag
     6961 cattctagaa acattgatgt accaacgttt ctaacagcta aaatagaaat tatttaactc
     7021 ttactgaagt taaataattg attataaaaa agcctatgaa atcaatattt ctgggctttt
     7081 tatttttaaa aataaaatct gacaaaaaag tataaattta cctcttacct tcaggtagtt
     7141 agtcattctt ttaaaactca cttttttata actgaaaata ttgttcttaa ttttaaaaat
     7201 tcattttaga ttcattttac gactatatac tcagaagtaa caaacctaat ccaaggtttg
     7261 aaaaaagaaa gaaggaagtc agtatgacaa actataaaaa caactttaaa aaattcatga
     7321 gcaataaatt atacgtaggg ttagcttcca ttacgctact tggtattggg ggagctatag
     7381 gagtaagtgg agctgcatta gcgcataacc atcactcaga acacatgtct tttgaacata
     7441 agcatgaagg acacgataaa catcacgatc atagagaaaa atactcacta aaaacattaa
     7501 agaatatctc gacaaaaatc gattataaga ctgccttaga aactgccaaa aaagaagctc
     7561 gctcaggctt tatttcacaa attgaattaa ataccaaagg tgatacacct ttttatgaaa
     7621 tcaaagttat tgataataaa atagctaaaa aatataaaat tgatgctaat tctgggaaga
     7681 tcctttcaac aacatcagat aatcaactca attcaaaaga tgcccaatta aaacaaccaa
     7741 gtaaagatat tcagactgca ttacaagcta ttcaagcaaa atataaaaat gcaaaaataa
     7801 aagaaatttc tcttgcgaat aacaaatcaa ataagctagt ttataaagta aaactacttg
     7861 atggaaatac tcctaagatt gcccaatttg atagtgataa agcatcattt ataaaggcaa
     7921 ataagcttaa tacaactcat aaaaatcacg actaatacta gtgtattaaa gggaatagga
     7981 cctcagtact ccattccctt ttgtgatata atggaaaaaa gccatacgga ggccaaaaaa
     8041 tgaaaatcct tgttgttgaa gatgagtttg acttgaaccg tagtattgta aaacttttaa
     8101 aaaagcaaca ctacagtgtt gacagtgctt caaatggtga agaagcctta caatttgttt
     8161 cagtggcaga atatgatgtg attatcttag atgtaatgat gccaaaaatg gacggcttca
     8221 cctttttaaa actgcttcgc aacaaaggaa gtcaagtatc tattcttatg ttgactgctc
     8281 gagatgccgt tgaagatcgt atcgcaggtc tagattttgg tgcggatgat taccttgtaa
     8341 aaccatttga atttggagaa ctcatggccc gaattcgagc tatgttacga cgtacaaata
     8401 gacaagtatc ttctgatgat attcaaattc aagatataac aatcaattta tctacaaagc
     8461 aagtttggag aaacgacaat ttgattgatt taacagctaa ggaatacgaa gtccttgagt
     8521 atttggcacg tcacagagac caagtcctct ctcgtcatca aattcgtgaa cacgtttggg
     8581 attatgatta ttatggagag tccaatatta ttgatgtcct tatcaaaaac cttcgtcgaa
     8641 aactagataa caatcgagat ggatcactaa taaaaactaa acgaggttta ggatatgtta
     8701 ttccaaagta aaataaatta ccctaaaaag tctagcatca tcactcaaat taccctctgg
     8761 tacagctctt ttatttttat ccttgttatc ggtgtattaa ttggttcatt ttttatttca
     8821 aaaagcattg ctgaaaacaa atctaaaaaa aatttagaag ctaaagctgt gcaaatgagt
     8881 caagcgttag ctaaaggtca tcggtatgag gcttttgaag atggaatatt ttattcagtc
     8941 tatgaccaaa atggaaaagt tatctattct ggttttccaa aaggatttaa aagggattta
     9001 gaccatcaac ataaacataa aaagaaatta tctctatttt ccatggagaa tcgtaccttt
     9061 cagtatgttg atattccaat ttctgggaaa aatcagtggt tacgtgctat ccgtactgtt
     9121 gacagactgg ataagcaatt aactgaattg ttatttagtt tgggtatcgt tcttcctctt
     9181 atgttgatta taataactgt tggtggctat ttaattttaa agcgcgcttt tagaccgatt
     9241 caagaaatta ctgaaaccgc tcaatttatt acccaaaatg aagactatac taagcgaatt
     9301 atcactaaaa ataacgaaaa tgaattaact gagctagcag ctgttatcaa tactatgttg
     9361 gcttctattg aatcatcatt tgttcgggaa aaacaattta acaatgatgt ttctcatgaa
     9421 ctacggactc ctgtaacagt tatactttct gaaagtgaat acggaaaaaa ctacgcagaa
     9481 aatttaagtg aagcaaaaga atcctttgag gttatacacc gtcagtcact atcaatgaaa
     9541 aaattagttg agcaacttct agaattgact aaggcagaaa atccattatc aattcagtta
     9601 gaacctctta atttctcaat tatgatgaag caactagtat ccgattcgag tagattatta
     9661 gataataccc ctatacacct tgactctcaa atcgaagatg acctttggat tataggtcaa
     9721 caaacactac tcaaacggct ttttgataat ctattttcaa atgctattaa atttacaaac
     9781 aatcatattt ctattagtct aagacagtcc gataatcaaa ttgtcttctc tattaaagat
     9841 aatggtctag gtatctcagt tgatgaccaa agtaaaattt ggaaccgttt ctatcaagtg
     9901 gactctgctc gaaccaagga tagtcagtct ggaattggtt tagggctatc tttagtaaaa
     9961 caaatcgcta caatacaccg tgctaaaata tgggttgatt ctaaacctga tgatggcagt
    10021 cagtttacgc tcacctttcc aaaattgaag aaagactaga gaaaggaaat aaaaaaaacg
    10081 atagattaca cctatcgttt tttttattta acttaaactc aatttattat atctcttcag
    10141 atatttttga gactaatagc tgaaatcttt aaagtattaa aacttgtaga actaacaaac
    10201 cgccaccaaa tattaggagg aataaaacaa ccggccacac aaattttagc caatgggagt
    10261 acttcatatc taacatctgc aaggttgcca taactagtcc agtaggtgct aaaaacaaca
    10321 ttgcatactg cccaaattga taagccatta ctactactga acgaggaatc ccaacagtat
    10381 ctgcaagagg tgctagaatt ggcattgaaa gtactgctaa acccgacgag gatggaacaa
    10441 caaatcctaa cacaaagtag ataaacatca taacaatgat aaagacaggg ccactaacat
    10501 gtgaagctaa ttttgaagct gtgtaaagca tcgtatctga tatataacct tggctcaaaa
    10561 taatattgat accacgcgct aaaccaatga ttaatgaaac cccaacaagg cttgatgcac
    10621 cattaacaaa ctcatcaaca acatcccttt ctccaatacc atttgcccca gttgctgtta
    10681 aaaacataat aataattgta atagctaaaa aggaagaagc catggttgga aaccaccatc
    10741 cctgtgacat tactccccaa accatcaaag gaaatgaaat aacaaataaa cttaaaataa
    10801 ttttttgtct aatagtaaag agtgagggtg tatggttagt tgtcatgccc catttagcat
    10861 taaattctac tctatcttca taggaatatg aaaactctgg attcgctttt atttttttag
    10921 cataccaatg tagatacacc acaacaaaaa tagcacctgc aatacaccct gctgtacgcc
    10981 atgataagcc ttctgtgaaa ctaataccag ctgcatttga agcaataaca gaagaaaacg
    11041 gattaattgt tgaaaatgat gtcccaacag aacttgctaa gaaaatagca cctacacaaa
    11101 tgatagaatc ataacccatg gctaaaaaga ttggaactaa aataggataa aaagcaactg
    11161 cttcttcttc aataccacat agagtaccgc ctaaaaccat caataaggaa actaaaaata
    11221 tcaataaaaa ctctctacct ttggttttct ttgttaaagc tagaagacca gactcaaaag
    11281 cgccactctt tctaactact cctatcatgc ctccaagtac catgataaat accataatat
    11341 caacagcttc aatggttcca tttaccatac tagtcgtcac ggagccaatg cctgctggat
    11401 tttgcttcaa acgtttgtaa gtatttggaa ctgaaactgg cttactaata gcaccagaag
    11461 taaattcttt aattttaatt ttgacattca tcttatccaa ctgtgtttgc gttgctggaa
    11521 catgaactgt cttaccattt ggatctgtta ctactagttt ggatgatgct gtatcaaatt
    11581 gtaattttga ataggaccca gcaggtatcc accatgtagc caaaaccgaa aagattgtta
    11641 aaatgaataa tattgtaaag gcacctggca ttcgaaaacc tttactcctt ttgttttcac
    11701 tgtttgtcat tttttctggc atgacacgcc tcctctataa taaaaataga ttgacttcat
    11761 cagaaaaagt gcaaagatga aggttaaata atcatttctt tgcacttatc tgagattact
    11821 tagttatacg tgttcctaat ttcccatcta atgcatcttc taaaccattt aatgaggtga
    11881 ttaaggctat tcctttcgga ttgtgatcta agaaactcat acaggctatt actttaggta
    11941 acatacttcc ttttgcaaac tcaccttggt taacgtaatc aatcatttga ttctcattaa
    12001 cctctgttaa agctttttga ttttctttac caaattgagt ataaacataa tcaactgcag
    12061 tgagaataat caattgatcc gcagctagtt ctcctgctaa cagtgcacta gatttgtctt
    12121 tatcaataac agctgcaata ccttcataac ctcccttatt aatgacaggg atgcctccac
    12181 ctccacctgc gataactagg gtatcatttt caataagtgt tttaatgcta ttaatttcga
    12241 tgattttttt aggttcaggt gaagcaacaa cacgacgata ccctcgtcca gcgtcttcta
    12301 cgaaagtata ccctttctct atagcaatct tttctgatgt ctccttatca taaaaggtgc
    12361 ctataggctt ggtaggtgcg ctaaatgctt ggtctgattc atccacctca atttgggtaa
    12421 taattgtagc aacttcctta tttattccct cttgacgtaa ttcatttaaa aggctctgtt
    12481 ggagatgata gcctatgtat ccttggctca ttgctccaca ttctggaaaa ggaaaattag
    12541 ttccttgacc actctcagcc gcaaaattca ttcctaaatt aatggcacca acttgtggtc
    12601 cattaccatg acttacaaca atttcgtgtc ccttttttat caaggccacc aatgattttg
    12661 cagtatgttt tactaatctt aattgttctt ctggagaatt tcctaaggca tttcctccta
    12721 gtgcaacaac aatttttgcc atgaaaattc ctcctaatca cctaaagttg ctaccataac
    12781 tgctttaata gtatgcatac gattttcagc ttcttggaaa acaactgagt gagggccttc
    12841 aaaaacttca tctgatactt ccatttcctt caaaccatat ttttcataaa tgtcgtatcc
    12901 aactttagta tctatattat gaaatgaggg caagcaatgc tcgaaaataa cgtttggatt
    12961 ttctgtcata ttaagcattt cttgggtaat acgatagggt tctaaaagag ctattctttc
    13021 tttccaaact tcatccggtt ctcccattga aacccaaaca tctgtatata ggacgtctga
    13081 atctctgacg ccttctgcaa tatcagcagt gatttcaata gaagcaccag tagttttcgc
    13141 aatttcttga catttagaga gtagttcagg atctggctct aattctttag gacaaaccaa
    13201 atgataagtc atgcctacaa tagaagcacc gatcatcaag gcattagcga cattgttacg
    13261 tccatcaccc acatacgtaa agcgaatatc tttatagggc ttatgtaagc attccttagc
    13321 ggtcagaaaa tcggctagta cttgcgttgg atgatcagca tcggttaaac cattccacac
    13381 aggaactcct gaaaattcgg ctaaagtctc aactgtttct tgagaaaatc cacgatactc
    13441 aataccatca tacataccac ctagcacacg cgcagtgtct tttgaggtct cttttttacc
    13501 catctgactg cctgttggac ctaaataagt aacatgagct ccttgatcgt gagcagcaac
    13561 ttcaaaagcg caacgtgtcc gtgtagaatc tttttcaaaa atcaaagcga tatttttacc
    13621 aacaagtttt tgttgttcaa ttcctgcgta tttagcacgt tttaaatctt ctgaaagttt
    13681 taaaagaaat tcaacttctg ctgtactaaa atccaataaa gtaagaaaac tacggttacg
    13741 taaatttttc attgcttatt gcctcctttt tttttatact cttattgtag atagcgcttt
    13801 caaaaataat caaaattcat caaaaatctt caaaaaaact ttaaggacat ttatatgcta
    13861 gatatattat cagaaaaaca tctagaaaga aggcagcgta ttattatctc tgcaatagct
    13921 atcgccttag ctgctcaaat caatatctca atacttgctg atgggtttat tatgacctta
    13981 tctttgttca ttttgcctgt ctttttatat tttaatgacg acattaatcc aattcttctc
    14041 tgtctaggaa ttacttttgc ttctcctatc ttcagaggaa tcatattaag tatcgctggc
    14101 gaggctgaga ttcatcaaat aattgaattc gttttaactg atatggcgtt ttatatttgt
    14161 tatggcatca ccttttatac tatttattgg catcgttcct accgtaataa aggtactttt
    14221 ttcttttcta tcattatttg tgattatttt gccaaccttg tagaaattag ttttctcata
    14281 aagtttaaca attatactat aactatattc gctactttat ttgccatagc acttttgcga
    14341 gcttttatta gttgcgctgt ggcttatact tatagttatc tatcacttct cttacaaaaa
    14401 gacgatcacg aacgtcgata ctattatttt atgtggtcaa cttcagccgt taaaagcgag
    14461 gtttatttta tgcaaaaaaa tatcattgaa attgaaaaca tcatgaaaaa tgcttattta
    14521 ttagataaag agttatccaa atatcaccta cctaaagaat accaacacct ctctctagat
    14581 atttcgcggg acgttcacga agttaagaaa gattaccaaa atattattaa aggcttagga
    14641 acctatttta gtgtaaaaaa tgaatcaact atggcattga aagatatctt tcaaattgtt
    14701 ttat
//
LOCUS       DATRQA010000043        14310 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.043, whole genome shotgun sequence.
ACCESSION   DATRQA010000043 DATRQA010000000
VERSION     DATRQA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14310
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.043"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1029)
                     /locus_tag="VBD27_000960"
     CDS             complement(<1..1029)
                     /locus_tag="VBD27_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO7024986.1"
                     /translation="MTLRQVTLKNTHQTSGIDKWISWLKKDISVRNRHKSKKLSLKKQ
                     RKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDRMNKALLEGKDLSKMLGELGFS
                     DTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGL
                     RHYLMPQLGENNFATRLITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTI
                     PLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQNQKSKLFREIGYDMEEGFLSG
                     KAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQP
                     VIFIFVALI"
     gene            complement(914..1846)
                     /gene="tadA"
                     /locus_tag="VBD27_000961"
                     /pseudo
     CDS             complement(914..1846)
                     /gene="tadA"
                     /locus_tag="VBD27_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            complement(2018..2383)
                     /locus_tag="VBD27_000962"
     CDS             complement(2018..2383)
                     /locus_tag="VBD27_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEO7024987.1"
                     /translation="MYQVVKMFGDWEPWWFIEGWEEDITEIAEYDTLSEALLYFQEEW
                     DRGQEKWPYFQSKSSLLATFWSIKEKRWCEECDEYLQQYHSLMLLKEWQEIPKEESIE
                     RFEVFNKIAELPSACSLNL"
     gene            complement(2497..6147)
                     /gene="rpoC"
                     /locus_tag="VBD27_000963"
     CDS             complement(2497..6147)
                     /gene="rpoC"
                     /locus_tag="VBD27_000963"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO7024988.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIICDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPMDTPLEPKSLLTEREYREKLQ
                     EYGYGSFVAKMGAEAIQDLLKRVDLDAEIAVLKEELKSATGQKRVKAVRRLDVLDAFK
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDLAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDHDEAVMAYQNGYVHLHTRVGIAVDSMPNKPWTEEQKHKIMVTTVGKI
                     LFNDIMPEDLPYLIEPNNANLTEKTPDKYFLEPGQDIQAVIDNLEINIPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     DINKAFRRGLMTEEDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLTITAITDGKEVTETLEERLIGRYTKKSIKHP
                     ETGEILVGADTLITEDMAAKVVKAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGEVVAIEEDSSTRTKKVFVKGQTGEGEYVVPFTARMKVEVGDEVARGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPLAVNEVEIIEGTPVDA
                     EVTEVSTPTTED"
     gene            complement(6264..9839)
                     /gene="rpoB"
                     /locus_tag="VBD27_000964"
     CDS             complement(6264..9839)
                     /gene="rpoB"
                     /locus_tag="VBD27_000964"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO7024989.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDAG
                     LKEVFEDVLPISNFTDTMDLEFVGYELKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDAKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGDSELVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNLKTRLLNQTIAENLVDGETGEILVEAGTVMTRDVIDSIAEHIDGDLNKF
                     VYTPNDYAVVTEPVILQKFKVVAPTDPDRVVTIVGNSNPEDKVRALTPADILAEMSYF
                     LNLAEGIGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRSTGAVTNEIV
                     WLTADEEDEFTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFPSSIVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAKH
                     DGRVIFSDAEKVEVRREDGSLDVYHVQKFRRSNSGTAYNQRTLVKVGDLVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEDSLRDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWETVQEAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDVMHVDDLEKARVKQEAEEKQAEQVSEVVQED"
     gene            complement(10363..12660)
                     /locus_tag="VBD27_000965"
     CDS             complement(10363..12660)
                     /locus_tag="VBD27_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7024990.1"
                     /translation="MFKGNKKLNSSKLGDYTPLEFGSIFLRIVKLLSDFIYVIILLFV
                     MLGVGLAVGYLASQVDSVKVPSKNSLVTQVNTLTRVSRLTYSDKSQISEIATDLQRTP
                     VAKDAISDNIKKAIIATEDENFNDHKGVVPKAVLRAAAGSVLGFGESSGGSTLTQQLL
                     KQQILGDDPSFKRKSKEIIYALALERYMDKDSILSDYLNVSPFGRNNKGQNIAGIEEA
                     AQGIFGVSAKDLTIPQAAFLAGLPQSPIVYSPYTADAQLKSDKDLSFGIKRQKNVLYN
                     MYRTRALTKDEYKSYKDYDIKKDFIKPAVATTNHHDYLYYSALSEAQKVMYNYLIKKD
                     NVSEHDLKNDETRATYRHRAIEEIQQGGYTIKTTINKSVYQAMQDAAAQYGGLLDDGT
                     GKVQMGNVLTDNSSGAIIGFIGGRNYSENQNNHAFDTARSPGSSIKPILPYGIAIDQG
                     MLGSGSVLSNYPTTYSSGEKIMHADEEGTAMVNLQESLDISWNIPAFWTYKMLRDRGV
                     DVKNYMEKLDYPIENFGIESLPLGGGIDTSVAQQTNLYQMIANGGVYHKQYMIESIED
                     SNGKVIYNHESKPVRVFSKATATILQQLLHGPINSGKTTTFKNRLQGLNSGLAGVDWI
                     GKTGTTNSTSDVWLMLSTPKVTLGGWAGHDNNASLAKLTGYNNNANYMAHLVNAINNA
                     DGNTFGKSERFRLDDSVIKAKVLKSTGLQPGVVTVNGRRITVGGESTTSYWAKNGPGT
                     MTYRFAIGGTDSDYQKAWSTLGGKR"
     gene            12771..14030
                     /locus_tag="VBD27_000966"
     CDS             12771..14030
                     /locus_tag="VBD27_000966"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO7024991.1"
                     /translation="MNIFDELKERGLVFQTTDEDALRKALEEGSVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDVERSLQTKKTVVSWGNKIR
                     GQLSNFLEFETGDNKAVLVNNYDWFSNISFIDFLRDVGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFYELNKNYNVTLQIGGSDQWGNMTAGTELIRRKSNGVSHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDADAVRFLKIFTFLSLKE
                     IEDIRIQFEEAPHQRLAQKTLAREVVTLVHGEKAYKEAVNITEQLFAGNIKGLSVKEL
                     KQGLRGVPNYHVQTEDNLNIIDLLVTSGVVNSKRQAREDVSNGAIYINGDRIQDLEYT
                     ISENDKLENEITVIRRGKKKYFVLNFK"
BASE COUNT         4465 a         2981 c         2402 g         4462 t
ORIGIN      
        1 gataagagct acaaaaataa aaataacggg ttggattaat tgggtcgctc tagctaattt
       61 tgtaaaaaaa tcctcccact tctcatcagc atatatatct aactctgttc ctaatttcgc
      121 cttaacttga ccatattcaa tcattaaact aagctcagtt aagaaaaacg gatagtctaa
      181 tactttttgg tgaaatgctt tacctgatag aaaaccttct tccatgtcat atcctatttc
      241 cctaaaaagt ttggatttct gattttgcat tactttaaca atttggtcca attcaacacc
      301 ttgacttaat aaatttcccc actcacgggc atagtaagca gttaaataaa gcttaacata
      361 tgatccaact aagggaattg ttgttaaaaa acaagctact ttaatgcgcg acaatcgctt
      421 ttgaataata taaaatatta aactaaaaat aagtacaact gctaaaagta ataagaaaat
      481 attcggcaca tttgtaatca gtctagttgc aaaattattt tctcctaatt ggggcattaa
      541 ataatgccta aggccaatca taattagcac cagaaaagac aataatatca atgggtaagt
      601 agctacttct attacttttt ttctaactaa caaaagatta gctaaataag actcaatctt
      661 tagtaggctc cttgaaatgt taccatgcaa atcagctaat gcaacctgtg tgataacagt
      721 gtcagaaaaa cctaactctc ctaacatttt tgataaatct tttccctcta ataatgcctt
      781 attcatccta tctgtataac aatcagacaa taacttactc ctctttaaga aggtgaccat
      841 atcagttaaa gaaaaaccac tagcaaaaag attattaaaa agttggacta ctttccgttg
      901 tttcttgagg gataattttt tcgacttgtg cctgtttctt actgatatgt ccttcttcag
      961 ccaagatatc cacttgtcta ttccacttgt ctgatgagtg ttttttaaag ttacttgtct
     1021 caaagtcaat taggcttcct cctccaatta aacgttgata tgctattaat tttagactat
     1081 tttctaactc ttgatagtta acccctaatt ctataagcct atcatagact ccgggaatac
     1141 ttttagcatg aatagtagaa aaaaccatta ctcccgttaa acttgcacga ataacagcac
     1201 gggccgtcgc ttgatctcta atctctccga taattaaaat atctggacga tgccgtaaag
     1261 acagtttgat taaagcatca taagtcattc caatatcctc attcaattgg agttgtaaca
     1321 tcttgtcatt cttgatttct accggatctt caatcgtgat aatttgctta tttttaaata
     1381 cttctgaagc taattgatac atgagagttg ttttaccact ccccacaggg ccggaaaaaa
     1441 gatatagccc tcttgcacac agtacttcct tcatttgctt tatattatca aaccaatatt
     1501 ttaagtcctg atgacctgaa tacaaagtac gaataactaa agattcttga ccacgataat
     1561 ctcccacact tgatagtcgt aatgaaacca gtcttccctc tgacagttca tagtcacaag
     1621 aacctaattg acttcgtctt ttttctccaa cgttcatgcc tgccacaaat ttaaagtgac
     1681 taataagact agccatccta ttaaactcaa aaacatcaat aaaccgcctt tcatcatcaa
     1741 tacgcatata gagttcataa caatcacctt tgggaatgat ataaatatct tgagcattta
     1801 cttctactgc ctgatgaatg acgtgctttg ctaatgattg aaccatcccg tacctcctta
     1861 accatatatt cgaaaataaa ataaaaaaat ctcaccaatt ggtgagattt tttcaatgat
     1921 ttcttgaggg gtagtaacat aaatattatg aagcagaggc tgaacaaacc tattatatcc
     1981 ccataaaaac tatctaactt ttttaaacta cttttaatta caaatttaaa gaacatgccg
     2041 atggcaactc agctatctta ttaaatactt caaatcgctc aatagattct tccttaggaa
     2101 tttcttgcca ttctttcaat aacattaagg aatgatactg ttggaggtac tcatcacatt
     2161 cttcacacca acgtttttct tttatactcc aaaacgttgc caacaaacta gacttacttt
     2221 gaaaataagg ccacttttcc tgtcccctat cccactcctc ttgaaaatag agcaaagctt
     2281 ctgataaggt atcatactca gcaatttcag taatatcttc ctcccaacct tcaataaacc
     2341 accatggttc ccaatcacca aacattttta caacttgata cataatacac ttcctttaga
     2401 gagtattata taaaaaataa gaataaaata aaaaatatct gctcattcca aattcttgta
     2461 aaaaaagaac ctgagttaaa ctcaagttct ttttgattaa tcttctgttg taggtgttga
     2521 tacttccgta acttctgcat caacaggagt tccttcaata atctcaactt cattaacagc
     2581 aagtggttca atattacggt aacgagccat accagtaccc gctggaatga ttttaccgat
     2641 aataacattt tctttaagac caagtaagtg atctttctta ccacgaatag cagcatctgt
     2701 aagaacacgt gttgtttctt ggaaggatgc agctgataag aatgaattag tttcaaggga
     2761 agccttagta atacccataa ggacaggacg gcttgtcgca gggattccac cagaaataac
     2821 aatatctttg ttagcatctg taaagtctga aatatccata agtgtacctg gaagaagatc
     2881 tgtatctcct ggatccatga cacgaacttt acggagcatt tgacgaacca ttacctcaac
     2941 gtgtttatct ccgatttcta ccccttggct acggtaaact ttttgtactt cagcaagtag
     3001 gtacgtttca acagataaag tatcacgaac ttcaagaaga cgttttggtt ggatagatcc
     3061 ttcagttaag gccgcaccac gagctacttc atcaccaact tcaactttca tacgtgctgt
     3121 aaatggaaca acgtattctc cctcacctgt ctgaccttta acgaatactt tcttagtacg
     3181 tgttgaagag tcttcttcaa ttgcaacgac ttcaccctta acttcagtaa tgactgcctc
     3241 acctttaggg ttacgtgctt caaagatttc ttggatacgt ggaagacctt gtgtaatatc
     3301 agtatttgaa gcaacaccac cggtatggaa ggtacgcatt gtaagctgtg tccctggttc
     3361 accgatagat tgagctgcaa tagtaccaac tgcttcacca acctcaacag catcacctgt
     3421 tgccaagtta ataccgtaac agtgacgaca aacaccatgg cgagtgttac atgtaaatac
     3481 tgatcggata gttacttcct caacgcccgc tttaacaact ttagcagcca tatcttcagt
     3541 aattaaggta tccgcaccaa cgagaatttc acctgtctca ggatgtttga ttgacttctt
     3601 agtataacgt cctatcaaac gctcttcaag tgtctcagta acctctttac catctgtaat
     3661 tgctgtgatt gtcaaaccgc ggtctgttcc acagtcatct tcacgaataa taacatcttg
     3721 ggcaacgtca accaaacgac gagtaaggta acctgagtcg gctgtcttaa gggccgtatc
     3781 agtcatacct ttacgcgcac cgtgagttga gaagaacatt tccaaaacac taagaccttc
     3841 acggaagttt gataagattg gcaattccat gatacgtccg ttaggagcag ccatcaaacc
     3901 acgcataccg gcaagttgtg agaagtttga gatgttacca cgagctcctg agtccatcat
     3961 cataacgata gggttctttg gatcttgtgt ctcaataaga cgtttttcaa gtgcttcttt
     4021 agcttcacgc catgttgttg taacagcaac ataacgatct tcttcagtca tcaaacctcg
     4081 acggaaggct ttattgatat cttcaactct gtggtgagct gcatcaataa tctcagcttt
     4141 attgtcaata actgggatat cagcaatacc cacagttaaa ccagcaagtg ttgaatgata
     4201 atatccaagg tccttcaaac ggtcaaggaa agcagaagtt tctgttgtac ggaaacgttt
     4261 gaaagtttct gcgataatat taccaagatt tttcttctta aagggaatat taatctctaa
     4321 gttatcaata actgcttgaa tatcctgacc tggctctaag aagtatttat ctggagtttt
     4381 ttctgtcaag tttgcattat taggttcaat taagtacggt aaatcttcag gcatgatatc
     4441 gttgaagaga atcttaccaa ctgttgtaac catgatttta tgtttttgct cttctgtcca
     4501 aggcttattc ggcattgaat caacagcgat accaacacgt gtatgtaaat gaacataacc
     4561 attttgatat gccataactg cttcgtcatg atctttgaaa atcatgcctt caccttcacg
     4621 accagcatct tccatagtca ggtagtaatt tcctaaaacc atgtcctgag atggtgttac
     4681 gactggctta ccatctttag ggttaaggat gtgctctgca gcaagcatca aaagacgagc
     4741 ttctgcttgg gcttcttcag acaatggaac gtggatggcc atttggtctc catcaaagtc
     4801 agcattgtag gcctcacata caagtggatg aagacgaagc gccttaccat caataaggac
     4861 aggttcaaac gcttggatac ctaatctgtg aagagtaggt gcacggttta gaagtactgg
     4921 gtgctcttta ataacttctt ctaagatgtc ccaaatacgc tcgtccccac gttcaaccat
     4981 acgtttagct gctttaacgt tccctgcgag gtcacgagca acgatttcac gcattacaaa
     5041 aggtttgaag agctcaatag ccatttcacg tggtacacca cattggtaca ttttaagtgt
     5101 tggaccaaca gcaataacgg aacgtccaga gaagtcaaca cgtttaccaa gcaagttttg
     5161 gcggaaacga ccttgtttac ctttaagcat atggcttaat gatttcaaag gacgactacc
     5221 tggtccggta attggacgac cacgacgacc attgtcgata agcgcatcaa cagcttcttg
     5281 taacatacgt ttttcatttt gaacgatgat gccaggagca tttaattcca acaaacgtgc
     5341 caaacgattg ttacggttaa taacacggcg gtaaaggtca ttcaagtctg atgccgcaaa
     5401 acgaccacca tctaattgaa ccatcggacg aagatccggt gggataaccg gtaaaatgtt
     5461 aagaaccatc cattctggtt tatttccaga ttttttaaag gcatctaaca catccagacg
     5521 acgaactgct ttcacacgtt tttgacctgt cgctgatttc aactcctctt taagaactgc
     5581 gatttctgca tctaaatcaa cgcgtttaag aagatcttga atggcttcag cacccatttt
     5641 agcaacaaac gaaccgtaac cgtactcttg aagtttttca cggtattcac gctcagtaag
     5701 gagtgacttc ggttctaatg gtgtgtccat tggatcgata acaacataag ccgcaaaata
     5761 aataacttct tcaagtgcac gaggactcat atccaatgtg agtcccatac gcgatgggat
     5821 acctttgaaa taccagatat gagatactgg tgcttttaac tcgatatgtc ccatacgttc
     5881 gcgacgaact tttgcacgag ttacttcaac gccacaacgg tcacaaatga ttcctttgta
     5941 acgaatacgt ttgtattttc cacacgcaca ttcccaatct tttgttggac caaaaataac
     6001 ttcatcaaaa agtccttcac gttctggttt caatgtgcgg tagttgattg tttcaggttt
     6061 tttaacttca ccataagacc atgaacggac cttacttggt gatgctaatg tgatttgcat
     6121 acttttaaaa cgatttacgt caaccactag ttttaccttt cttcgattta tcatcatact
     6181 agcccagcta ggatttcaat aaatcctagg actagcattg catatcaatt ctatattcgc
     6241 ttctagttat aaactagata aatttaatct tcttgaacga cttcagatac ttgttcagct
     6301 tgtttttctt cagcttcttg tttcacacgc gctttttcaa ggtcatcaac gtgcatcaca
     6361 tcatcatctt caccttcatc aagatcacgg agttcaactt cgttgtcatc ttcatcaagg
     6421 acacgcatat caagaccaag tgattgcaat tcttttacaa gaacacggaa tgattctgga
     6481 acacctggtt ttggaattgg cttacctttt gtgatagctt cataagcttt aagacgtcct
     6541 gtaacatcat cagatttgta tgttaaaatt tcttgaagaa catttgaagc tccataagct
     6601 tcaagcgccc aaacctccat ctcaccaaaa cgttgtccac caaattgagc tttacctcca
     6661 agtggttgtt gagtaacaag tgagtatggt cctactgaac gggcatgaag tttatcatca
     6721 accatgtggt gaagtttgat catgtacatg acaccaaccg acacacggtt atcgaatggt
     6781 tcaccagtac gaccatcata aagaacagtc ttagcatctg aatccatacc tgcttcttga
     6841 acagtttccc aaaggtcttc tgaagaagcc ccatcaaaga ctggtgttgc aatatgaata
     6901 cctaaattac gagcagccat acctaaatga agctccataa cttgtccgat attcatacgt
     6961 gatggcaccc caagtgggtt caacatgata tcaactggcg ttccatctgg taaataaggc
     7021 atatcttcaa caggaacaat acgtgagacg acacctttgt ttccgtgacg accggccatc
     7081 ttatcaccga ctttgatttt acgtttttga gcgatataaa cgcggacaag catattaaca
     7141 cctgattgca attcatcacc atttgcacga gtaaagattt taacgtcacg aactactcca
     7201 tcgccaccgt gaggtacacg tagtgaagta tcacgaactt cacgtgattt atcaccaaaa
     7261 atggcatgca agagacgttc ttcagcagat aagtcctttt cacccttagg cgttacctta
     7321 ccaacaagaa tgtcaccttc ttttacctca gcaccaatgc ggataattcc catttcatcg
     7381 agatcacgta gtgaatcttc accaacattt ggaatttcac gagtaatttc ttcaggccca
     7441 agctttgtat cacgcgtttc agattcgaat tcttccaagt gaacagatgt atagacgtct
     7501 tctttcacaa ggcgctcact catgataacg gcatcctcga agttataacc ttcccaagtc
     7561 atataagcaa caactgggtt ttgtccaaga gccatttcac cattttccat agatggtccg
     7621 tcggcgataa aatcaccttt ttcaacgaga tcaccaactt taactaaggt acgttggtta
     7681 taagcagtac ctgagtttga acggcggaat ttttgaacat gataaacatc aagagaacca
     7741 tcttcgcgac gcacttcaac tttttcagca tctgaaaaaa taacacgacc gtcatgttta
     7801 gcaatcacag ctgcacctga atcgtgggct gcttgatact ccataccagt accaacatat
     7861 ggtgcttttg gatcaatcaa tggcacagcc tgacgttgca tgttggcacc catcaaggca
     7921 cggttagagt catcattttc caagaaagga atacatgctg ttgcgacagc aactacttgc
     7981 ttaggtgaca cgtcaacaaa gtcaacaatg cttgaaggaa actcttggtt attaccttga
     8041 tgacgaccca taacgatttc ttctgcaaat gtaccgtctt cgttaagctt agagttggct
     8101 tgtgcaactg taaattcatc ttcttcatca gcagtcaacc aaacaatttc gttcgttacc
     8161 gcaccagtag aacgatctac tttacgatat ggtgtttgaa taaatccata tttattgagg
     8221 tgtccaaatg aagataagtt attaatcaaa ccaatgtttg gaccttcagg tgtttcaatt
     8281 ggacacatgc gcccatagtg ggtatagtgc acgtcacgaa cttcatatcc agcacggtca
     8341 cgtgtcaaac caccaggtcc taaggcagag agacggcgtt tgtgcgacaa ttctgataga
     8401 gggttgtgtt ggtccatgaa ttgtgacagc tgtgatgaac caaagaattc tttaacagcg
     8461 gcagtcactg gacgaatgtt gatgatttgt tgaggtgtca agacttcatt gtcttgtaca
     8521 gacatacgtt cgcggacatt gcgttccata cgagcaagtc cgatacggaa ttggttcgca
     8581 agtaattcac caactgcacg aatacgacgg ttacctaaat ggtcaatgtc atctacctta
     8641 ccgataccct ctgcaaggtt taagaagtat gacatctctg ctaaaatatc tgcaggagtt
     8701 aaggcacgaa ctttatcttc aggatttgag tttccaacaa tagtaacaac acggtctgga
     8761 tcagttggag caacaacttt gaatttttga agaataactg gttcagtaac tacagcataa
     8821 tcatttggtg tgtagacaaa cttgtttaag tctccatcga tatgctctgc aattgaatca
     8881 atgacatctc gtgtcataac ggttccagct tcaacaagaa tctctcctgt ttcaccatca
     8941 actaaatttt cagcgattgt ttggttcaat aggcgtgttt taagattcag ttttttatta
     9001 atcttataac gaccaacagc cgctaagtca taacgacgtg gatcaaagaa acgtgcaact
     9061 aaaagactac gtgagctgtc tgcagttttt ggttcacctg gacgaagacg ttcatagatt
     9121 tctttaagtg cttcatctgt acgagaatca cttggatttt tatgaatgtc tttttcaata
     9181 gtattgcgaa caagttcact gtcaccaaag atgtcaacaa tttcatcgtc tccagagaaa
     9241 ccaagtgcac ggactagtgt tgtaaatgga attttacgtg tgcggtcgat acgtgtataa
     9301 gcaatatctt ttgcgtctgt ctcaagttcc aaccaagctc cacggttagg gataacagtt
     9361 gacccataac caacttttcc atttttatct accttatcat taaagtaaac accaggagaa
     9421 cggactaatt gtgaaacaat gatacgttca ccaccattga tgatgaaagt acccatttca
     9481 gtcataattg gaaaatcacc aaagaaaact tcttgagttt taatttcacc agtttcttta
     9541 ttaactaaac ggaaagtaac aaaaataggt gctgagtagc tagcatcatg gatacgagct
     9601 tcttccaaag tatatttagg ttcttttaat tcataaccaa caaattcaag atccatagta
     9661 tctgtaaagt ttgaaattgg aagtacatcc tcaaatactt ctttcaagcc agcatcaaga
     9721 aaatcttgga atgaatcagt ttgaatttca attaagtttg gtaaatcaag aacttcctta
     9781 attcttgaaa agctacgacg tgtacggtgt tttccgtact gaacttcatg tcctgccaag
     9841 tttttagctc ctttttaaag atagagtgac taagttttta tctcataaaa cttagtgagt
     9901 ttatttcatt tgctcgccta acaagaacaa gcttatagat gtcgtttgtg gtaattttca
     9961 gaaagtttaa atatatatta caataacctt ttttcttaaa attaggcaca aaaagagcag
    10021 ctaaatctca ctttatcaag ttgatttaac tgctgtatat ctaggtcgga caatatttca
    10081 acctagacag acgacaaata gttacgtaca ttataactta ttttcaccaa taaatcaagt
    10141 tttattctct tgacttcaag gaagcttcta gaatttcaat ttgttcatca acacccttga
    10201 gttttagcat aacacgatga tttttaaaca tagcatactg gtgtttttct aattctttga
    10261 aagaagacta tacaagaatt aaatcatatt atcttggaaa aaataaaagg agaacagatc
    10321 actctattct cctcaaataa aactatcagt atcgtaaacc tgttatcgtt ttccacccaa
    10381 agtagaccaa gctttttgat aatcactatc agttccccca atagcaaaac gataggtcat
    10441 cgttccaggt ccattttttg cccaataact tgttgtgctt tcacctccaa cagtgattcg
    10501 acgaccgttt actgttacta cacctggctg taaacctgta gacttaagaa ctttagcttt
    10561 tataacgcta tcatcaagtc taaacctttc tgacttacca aaagtattgc catctgcatt
    10621 attaatagca ttaaccaagt gagccatata attagcatta ttattgtaac ctgtcaattt
    10681 cgctaaagag gcattattat catgtcctgc ccatccacct aaagtcactt taggtgtaga
    10741 aagcatcaac caaacatcag aagttgagtt agtagtaccc gtttttccaa tccaatctac
    10801 cccagctaaa ccactattta aaccttgtaa gcgattttta aaggttgtag tctttccgga
    10861 gttaattggt ccatgtaata gctgctgtaa gatagtagct gttgctttcg aaaagacacg
    10921 gacaggttta ctttcgtgat tataaatcac tttcccatta ctatcctcta tactttctat
    10981 catatactgc ttatgataaa caccaccatt tgcaatcatt tgataaaggt ttgtttgctg
    11041 agcaactgac gtatcaatac caccaccaag tggtaaactt tctataccaa agttttcaat
    11101 tggatagtct aatttctcca tatagttttt tacatcaacg cctcgatctc gtagcatctt
    11161 gtaagtccag aaagctggaa tgttccaaga aatgtccaat gactcttgaa ggttaaccat
    11221 agcagtacct tcttcatccg catgcataat cttttcgcca cttgaatatg tcgttggata
    11281 atttgaaaga acactaccac ttcctaacat cccttgatca atagcaattc cgtaaggtaa
    11341 tatcggttta atacttgacc ctggagagcg tgccgtatca aaagcatggt tattttgatt
    11401 ctcactatag ttacgaccac caataaatcc tataatagca ccactagaat tgtccgttag
    11461 tacatttccc atttgaacct tgccagtacc atcatctaac aaccctccgt attgagctgc
    11521 agcatcctgc atggcttgat aaacagattt attgatagta gttttaatag tataccctcc
    11581 ctgttgaatc tcttcaatag cgcggtgtct gtacgtcgct ctagtctcat cattttttaa
    11641 atcatgctct gaaacattat cctttttaat gaggtaattg tacatcactt tttgagcttc
    11701 agataatgcc gagtagtaaa gataatcatg atgatttgtt gtcgccactg caggtttgat
    11761 aaaatctttt ttgatgtcat aatctttgta ggatttgtac tcatctttgg ttaaagctct
    11821 cgttctatac atattgtata acacattttt ttggcgcttt ataccaaaac taagatcttt
    11881 atctgatttc aactgggcat ctgcagtata aggagaatat acaataggac tttgcggtaa
    11941 acctgcaagg aaagctgcct gaggaattgt taaatctttt gcggaaactc caaaaatccc
    12001 ttgtgctgct tcttcaatac ctgcaatatt ctgtcctttg ttattgcgac caaacggtga
    12061 tacatttaaa taatctgata aaatcgaatc cttatccatg taacgctcta atgctagagc
    12121 ataaataatt tcttttgatt tgcgtttaaa tgacggatca tctcccaaaa tttgctgctt
    12181 taataattgt tgtgtcagcg ttgaaccacc gctactctct ccaaacccca gaacagatcc
    12241 cgctgcagca cgtagtacgg ctttgggaac tacaccttta tggtcattga aattttcgtc
    12301 ctctgttgca atgatagcct ttttaatatt atcagatata gcatcctttg ccactggtgt
    12361 acgttgtaaa tcagtggcaa tctctgaaat ttgtgattta tcagaataag ttaacctaga
    12421 caccctagta agtgtattaa cttgtgtcac taaactattt ttacttggta ctttaacaga
    12481 gtcaacttga ctagcaagat accctactgc aagtcctact ccgagcataa caaatagtaa
    12541 aataataaca tagatgaaat ctgataaaag ctttacaatt cttaaaaaaa tagaaccaaa
    12601 ttcaagtggt gtgtaatcac ctaatttaga actattcaac ttcttattac ctttaaacat
    12661 gaataatacc tcatcctaca ttatatcaga tttaatataa cttgcatatc ctaaagagtt
    12721 ttgatatagt tgacattaat acacaacaag ttaataatta ggagaaaaag atgaatattt
    12781 ttgatgaact caaagaacgt ggactcgttt ttcagacaac tgacgaagat gctttacgca
    12841 aagctcttga agaaggttct gtctcttact atactggcta cgatcctacg gctgacagcc
    12901 ttcatcttgg acatttagtt gctattttaa cctctcgtcg cttacaactt gctggtcaca
    12961 agccttatgc acttgtggga ggcgcaactg gacttattgg ggacccttca tttaaagatg
    13021 ttgagcgcag tttacaaaca aagaaaactg tagttagttg gggtaataaa atccgcggtc
    13081 aattatctaa ttttctagag tttgaaacag gtgataacaa agctgtattg gtaaataatt
    13141 atgattggtt cagcaatatt agtttcattg atttcttacg agatgttggt aaatatttta
    13201 ctgtcaatta tatgatgagt aaagaatctg ttaaaaaacg tatcgaaaca gggatttcct
    13261 atactgaatt tgcttatcaa attatgcaag gatatgactt ttacgaatta aataagaact
    13321 ataatgtcac tttgcaaatt ggtggttctg accaatgggg caacatgact gctggaaccg
    13381 aacttattag acgaaaatca aatggtgtat ctcatgttat gactgttcca ttgataacag
    13441 attctacagg aaaaaaattc ggaaaatctg aaggaaatgc tgtttggctt gatgctgaca
    13501 aaacctctcc ttatgaaatg taccaatttt ggctcaatgt tatggatgct gatgccgttc
    13561 gcttccttaa gatttttaca ttcctaagcc ttaaagaaat agaagatata cgtattcaat
    13621 tcgaggaagc acctcaccaa cgcttagcac aaaaaacact agcacgtgaa gttgtaactc
    13681 ttgttcatgg tgaaaaagct tataaggagg ccgttaatat cacggaacag ctatttgctg
    13741 gcaatataaa aggattgtct gttaaagagc tcaaacaagg tttacgtgga gtaccaaatt
    13801 atcacgttca gacggaagat aacctcaata tcatagacct tctagtcact tcaggagttg
    13861 tcaattctaa acgtcaagct cgagaagatg tctccaatgg agctatttat attaatggag
    13921 atcgtattca agatttagaa tatactatct cagaaaatga taagcttgaa aatgaaatta
    13981 cagtcattcg tcgtggaaag aaaaaatact ttgtattaaa ctttaaatag acttgaagtg
    14041 taatcgatac tctgataata atatttcaag acaataaaaa gtgtagtcaa ttgactacac
    14101 tttttattaa tgaacaacat tcctgaagag acaactttta aattatagtt attatacatg
    14161 actaccttat ttttattaag aaataacacc aaacaaacag cttagaattt ttaccataga
    14221 gtaaatagtt atcgaaaaaa tatacatatc taatacccac aattttttca taaaaacaac
    14281 aaatatcgtc tgtctaacac caacgaattt
//
LOCUS       DATRQA010000044        13936 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.044, whole genome shotgun sequence.
ACCESSION   DATRQA010000044 DATRQA010000000
VERSION     DATRQA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13936
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.044"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            63..2627
                     /locus_tag="VBD27_000967"
     CDS             63..2627
                     /locus_tag="VBD27_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HEO7024992.1"
                     /translation="MRPIVRKKMYKKGKFLVIAGIVTILGGSAILGQDVKAEQAEAVT
                     STISEKTDSSQTISDISKLTLPVNSSEAMKNSAEPLIKTGFATSVSSNPREIAATPVK
                     TFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPTVTYSAHVQDIG
                     WQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQPSVESGQVS
                     GTVGQSRPIEALSINLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLGMSKRLEA
                     YKVKFTLKGQSVLTPTILKEERPVLNYQVKVGQNGWQSNKLEGQMAGTLGESKALDGV
                     KFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNLKQNYDIY
                     YRAHVQDKGWMAWQKNNSVAGTVGESKRLEALEVKLVAKASDFSSQTNHSFLEMKHPG
                     LSYQTYLQKDGWKPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNGSGWQ
                     TVVNSGRESNVPNESQQVEAIQMRLTGLLSKTYSVMYRVHMQDYGWQDWTYNNDIAGS
                     TNQGKRIEAIEIKLVETAKMPITVQTTYKGTGNYNVRVTNVISPGNLKIAIWSDKNNQ
                     DDLKWYTVTPKNHEARLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNY
                     NTPYFSQRDGRWASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQIADYLYHKTV
                     EFNRGVQGTTSRGILKAAKEWGVTATALGTQANLVQALKDGYHVLAAVQNNVFVLHGS
                     HEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGAPFVKVTDA"
     gene            2750..3268
                     /locus_tag="VBD27_000968"
     CDS             2750..3268
                     /locus_tag="VBD27_000968"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEO7024993.1"
                     /translation="MDLNNYIASIENYPQEGITFRDISPLMADGKAYSYAVREIVQYA
                     ADKDIDMIVGPEARGFIVGCPVAYALGIGFAPVRKPGKLPREVISADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVVAGCAFLVELDGLNGRKAIE
                     GYDTKVLMNFPG"
     gene            3386..4066
                     /locus_tag="VBD27_000969"
     CDS             3386..4066
                     /locus_tag="VBD27_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEO7024994.1"
                     /translation="MTYLEQYQSGQLTLPSALFFHFKSIFKTADDFLVWQFFYLQNTT
                     NLSDLTPSRIATSLDKTVADINRSISNLTSQGLLDVKTIELNHEIEIIFDTSPVFAKL
                     DKLFEEDNQVIIDNKTADSNRLKDLVGDFERELGRLLSPFELEDLQKTLQEDQTDPDI
                     VRAALREAVFNGKTSWNYINAILRNWRREGLTTLRQIEERKQAREDNQMKDLAISDDF
                     KNAMNLWE"
     gene            4170..4853
                     /locus_tag="VBD27_000970"
     CDS             4170..4853
                     /locus_tag="VBD27_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEO7024995.1"
                     /translation="MDLQLSKRLQKVANYVPKGARLLDVGSDHAYLPIFLLQMGYCDF
                     AIAGEVVNGPYQSALKNVSEHGLTSKIDVRLANGLSAFEEADNIDTIAICGMGGRLIA
                     DILNNDIDKLQHVKTLVLQPNNREDDLRKWLAANDFEIVAEDILTENDKCYEILVVKH
                     GHMNLTAKELRFGPFLLSNNTTVFKEKWQNELNKLTFALNSIPNSKMEERAILEDKIQ
                     DIKEVLDES"
     gene            4843..5631
                     /locus_tag="VBD27_000971"
     CDS             4843..5631
                     /locus_tag="VBD27_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO7024996.1"
                     /translation="MKARELIDVYETYCPQELSMEGDISGLQIGSLDKEIKTVMVALD
                     VRETTVAEAIERQVDLLIVKHAPIFRPLKDLVATPQNKIYIDLLKSDIAVYVSHTNID
                     IVPNGLNDWFCELLDIQYPDILSETSNGYGIGRIGDVRPQSFEFFAWKIKDVFGLDSV
                     RLVSYDKSNPEIQRVAICGGSGQSFYKEAIAKGADVFVTGDIYYHTAQEMITNGLLAI
                     DPGHHIEVLFVSKIATMIEQWKLEKGWDISVLESKAPTNPFYHM"
     gene            5640..6743
                     /locus_tag="VBD27_000972"
     CDS             5640..6743
                     /locus_tag="VBD27_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO7024997.1"
                     /translation="MKKIAIIGAGAVGATLAYYLSKEKDIQVTVFDYGVGQATKAAAG
                     IISPWFSKRRNKAWYRMARLGADFYSKLVTDLQKDGFETKFYQQTGVFLLKKDESQLE
                     SLFALADKRRLESPLIGDLQILNKSEANTHFPELDGYEQLLYASGGARVEGADLTRIL
                     LEASGVNVIKDEVHFTITDNGFRVQGIDFDKLVLASGAWLAKILDEHNYQVDIRPQKG
                     QLRDYYFSNINTGKYPVVMPEGELDIIPFDNGKVSVGASHENDMAFDLNIDFKVLDKF
                     EEQAIGYFPQLKKADTTSERVGIRAYTSDFSPFFGPVPCMEGAYAASGLGSTGLTVGP
                     LIGYELCQLILNKENQLNLEDYDITKYVKKISR"
     gene            6802..7671
                     /gene="rfbA"
                     /locus_tag="VBD27_000973"
     CDS             6802..7671
                     /gene="rfbA"
                     /locus_tag="VBD27_000973"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO7024998.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSVLMLA
                     GIKEILIISTPQDLPRFEDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDHV
                     ALVLGDNIYHGPGLSAMLQRAASKESGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PAQPKSNYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRMQNVQVANLEEIAYRMGYITREQVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            7671..8264
                     /locus_tag="VBD27_000974"
     CDS             7671..8264
                     /locus_tag="VBD27_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEO7024999.1"
                     /translation="MTEQFFDKELTCRPIEAIPGLLEFDITVRGDNRGWFKENFQKEK
                     MIPLGFPESFFEADKLQNNISFNKKNTLRGLHAEPWDKYVSIADEGRVIGTWVDLREG
                     DSFGNVYQTIIDASKGIFVPRGVANGFQVLSDKAAYTYLVNDYWALELKPKYAFVNYA
                     DPNLGIQWENLEEAEVSEADKNHPLLKDVKPLKKEDL"
     gene            8471..9517
                     /gene="rfbB"
                     /locus_tag="VBD27_000975"
     CDS             8471..9517
                     /gene="rfbB"
                     /locus_tag="VBD27_000975"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO7025000.1"
                     /translation="MTEFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     RANIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGNGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKD
                     AVEAKYAKTQEVIK"
     gene            complement(9569..12787)
                     /gene="hylB"
                     /locus_tag="VBD27_000976"
     CDS             complement(9569..12787)
                     /gene="hylB"
                     /locus_tag="VBD27_000976"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEO7025001.1"
                     /translation="MEIKKKHRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRD
                     TQTNNIEVLQTESTTVKETSTTTTQQDLSNPTASTATATATHSTMKQVVDNQTQNKEL
                     VKNGDFNQTNPVSGSWSHTSAREWSAWIDKGNTADKSPIIQRTEQGQVSLSSDKGFRG
                     AVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTL
                     TKIYNPKLNVSEVTLELYYEKGTGFVTFDNISMKTKGPKDSEHPQPVTTQIEKSVNTA
                     LNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTA
                     STEDNFTKLLDKWNDVTIGNYVYDTNDSNMQKLNQKLDETNAKNIEAIKLDSNRTFLW
                     KDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVN
                     KDIEGSANWWDFEIGVPRSITGTLSLMNNYFTDAEIKTYTDPIEHFVPDAEYFRKTLV
                     NPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYID
                     HTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELM
                     DMSRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVF
                     NNLKSYSDIANMNKLLNDSTVATKPLKSNLSTFNSMDRLAYYNAKKDFGFALSLHSKR
                     TLNYEGMNDENTRGWYTGDGMFYIYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDT
                     TKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVIL
                     NDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTK
                     SVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKH
                     DNKGDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQE
                     SLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI"
     gene            13004..13486
                     /locus_tag="VBD27_000977"
     CDS             13004..13486
                     /locus_tag="VBD27_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO7025002.1"
                     /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGE
                     TPDECAKREILEETHLTVKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDES
                     REGTLEWVPYDQVLSKPTWQGDYEIFKWILEDVPFFSAKFVYDEHQNLIEKTVNFYEK
                     "
     gene            13476..>13936
                     /locus_tag="VBD27_000978"
     CDS             13476..>13936
                     /locus_tag="VBD27_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7025003.1"
                     /translation="MKNRDFRVRDQLETFGVRVSALIIENQKLLLIYAPHLYKYYLPG
                     GALQVGEDSNKAVAREVLEEIGLHSQVGDLAYIVENQFNIKRHHYHSVEFLYFVNLLG
                     QAPESIKEGTHKRHFVWLPIKELTKIDCNPNFLAQDLIEWPGHVVHKIVQN"
BASE COUNT         4505 a         2201 c         2825 g         4405 t
ORIGIN      
        1 taataaaatt aagaaaataa tggaattttt gataaagaat attaaaggtg aggataaaat
       61 ctatgagacc tatcgttaga aaaaagatgt acaaaaaagg taaatttttg gtcattgcag
      121 gtatcgttac tattttaggt ggtagtgcaa ttcttggtca agatgtcaag gcagaacagg
      181 cagaagcagt aacttcaacg atctcagaga aaactgatag cagtcagact atttcggata
      241 tttccaagtt aacgttacct gttaattcct cggaggctat gaaaaatagt gcagagcctt
      301 taataaaaac aggctttgct acatctgttt catctaatcc tagagagata gcagctaccc
      361 cagtcaaaac ctttgatgct agttcaaaag ttgttgtaaa agctagtact gctgagcact
      421 cagcaaatca aacaaattct aatgttaatc aagtagctaa tgatagtgaa gttattactc
      481 aacaaaattc aacaaagcaa ttaccaacag tgacttactc tgctcatgtt caagatattg
      541 gatggcaaaa gtcggttgat aatgcaacag tttcaggaac agtaggacag gaaaagcagg
      601 ttgaggcaat aaaattgtct attaaagccc ctgaaggaat aacaggaaaa cttagctata
      661 aaacttatgt taaaggacaa ggttggcaac cttcagtaga gagtggtcaa gttagtggta
      721 ctgttggtca gagtagacct attgaagcac taagcattaa ccttactgat aatttacaaa
      781 aattatatga tgtctattat cgtgttcatg ttcaagatat cgggtggatg gcttgggcta
      841 aaaatggagc ttacgctggg actttgggaa tgtcaaagcg ccttgaagct tacaaagtaa
      901 agtttaccct taaaggacaa tctgttttaa caccaactat acttaaggaa gagagacctg
      961 tcttaaatta tcaggttaag gtaggccaaa atggttggca atctaataaa cttgaaggac
     1021 aaatggcggg aactttagga gaatccaaag ccctagatgg agtaaaattc actcttagca
     1081 ccttgaagta tggggacatt ctctatcgta ctcatgtaca agataaaggt tggggagaaa
     1141 ttttaggtag tcaaacaagt aaggattatc aaggaaaaag actagaagct attcaattgg
     1201 acttaagtgg gaatttaaaa caaaattatg atatttatta tcgcgctcac gtccaagata
     1261 aaggttggat ggcttggcaa aaaaataata gtgtagcagg tacagtcgga gaatcgaaac
     1321 gtttagaggc tttggaagta aaactagtag ctaaagcttc agattttagt tcccaaacaa
     1381 atcattcatt cttagaaatg aagcatcctg gattaagtta tcaaacttat cttcaaaaag
     1441 atggttggaa accaacggtt ttggaaggcc aattaggagg tagtattgga ctttcaaaat
     1501 ctataaaagc catcaaactt aacttaggta gtacagctct aggaaatata gaatatagaa
     1561 cttttcttaa tggttcaggt tggcaaactg ttgttaattc agggagagaa agtaatgtac
     1621 caaatgaatc tcaacaagta gaagctattc agatgcgatt gacaggtctg ctatcgaaaa
     1681 cctatagtgt gatgtatcga gttcacatgc aagattatgg ttggcaggat tggacttata
     1741 acaatgacat cgctggtagt actaaccaag gaaaacgaat tgaagcaatc gaaattaaat
     1801 tagttgagac tgctaaaatg cctataactg tccaaacgac ctataaggga actggaaact
     1861 ataatgtacg agtgacaaat gttattagtc ctgggaattt aaaaattgct atttggtcag
     1921 ataaaaacaa tcaggatgac cttaagtggt ataccgtcac acccaaaaat catgaagcta
     1981 gattgacatt taatgttaca aatcatagag acaatggtaa atactttatc catgtttatc
     2041 aggaaaatgc ttctaatcaa aaacaattat tggtaaaaac ttctctacaa gtcgcacatt
     2101 caaattataa tactccttac tttagccaac gtgatggaag gtgggcaagt cgaagatatg
     2161 gtatggcaac tttaggagaa actggttgtg taccaacttc tctagctatg attttgtctt
     2221 ctttgaaagg agaaactgtt ttaccaacgc aaattgcaga ttacctttac cataagacag
     2281 ttgaatttaa tcgtggtgtt caaggaacaa cttctagagg aatcttaaaa gcagccaaag
     2341 aatggggtgt aacagcaact gcattaggaa ctcaagctaa ccttgtacaa gcgttgaagg
     2401 atggttatca tgtcctagcg gcagtacaaa ataatgtttt tgttcttcat ggaagtcatg
     2461 aaatcgttct caaaggatat aataacggac tgacacatgt ctctgaccct tacacaccgt
     2521 ccttgtctgg ttggtatcca atttcacaac tttggaaaga acagagctat tatagtgagg
     2581 atagaattga tattggagca ccttttgtca aggtaacaga tgcctaattt ttaaaaccat
     2641 atgcaaaact agcatatggt tttaaagttt ccagtactat tttatttaat tttatgatat
     2701 aatgaagagt aaaacatttt aggggtttcc ccttagaaag acaagtgaaa tggatttaaa
     2761 taattacatc gcttcaattg aaaactatcc tcaagaagga attactttcc gtgatatctc
     2821 cccattaatg gctgatggta aagcatacag ctatgctgtt cgtgaaattg tacaatatgc
     2881 tgctgataaa gatattgata tgattgttgg accagaagct cgtggcttta ttgttggttg
     2941 cccagttgct tatgctcttg gtattggttt tgctccagtc cgtaaaccag gtaaattacc
     3001 acgcgaagtt atttcagctg actatgaaaa agaatatggt cttgatacat tgacaatgca
     3061 cgcagatgct attaaaccag gtcaacgtgt tttaattgtt gatgaccttt tagctactgg
     3121 tggaacagta aaagcgacaa ttgaaatgat tgaaaaactt ggtggcgttg tcgcaggctg
     3181 tgctttccta gttgaacttg atggattaaa tggtcgtaaa gctattgaag gttatgatac
     3241 taaggtattg atgaactttc caggataagg cctattcaag atagatgaat gataagggcg
     3301 cctctaagtg ctatgataat agttgagagg tgctttttgt tacacatttc ataattttag
     3361 gacgatagag aggatttgaa aggttatgac ttatttggaa caatatcagt cagggcaatt
     3421 gactctacca agtgctttat tttttcactt taaatctatt tttaagacag cagacgattt
     3481 tttagtttgg caatttttct atttacaaaa tacaactaat ttatccgatc taacaccgag
     3541 tcgtattgcg acatcacttg ataaaacagt ggcagatatc aatcgttcta tatcgaattt
     3601 aacaagtcaa ggtttactag atgtcaaaac aattgagttg aatcacgaaa tcgagattat
     3661 ctttgataca agccctgtgt ttgctaaatt agataaactt ttcgaagagg ataatcaagt
     3721 aataatagac aataaaaccg ctgattcgaa tcgtctgaaa gatttagtag gagacttcga
     3781 acgtgaatta ggtcgcttat taagtccttt tgaattagaa gatttacaaa agacactgca
     3841 agaagatcag acagatcctg atattgttag ggcagctctt agagaagcgg ttttcaatgg
     3901 gaaaacaagt tggaattata tcaatgctat ccttcgcaac tggcggcgtg aaggattgac
     3961 gacacttcga caaattgaag agagaaaaca agcacgtgaa gataaccaaa tgaaagattt
     4021 agcgatttcg gatgatttta aaaatgcaat gaatttatgg gaataagttt actaggagga
     4081 gacagaatgg ttgatacatt aggtctgctc cttttctttt gatgttaatg atgttatact
     4141 tttaacagag aaaaactgaa ggaaatacaa tggatttaca attatcaaaa cgattacaaa
     4201 aagttgcaaa ctatgttcca aaaggagctc ggttattaga tgtaggaagt gatcatgctt
     4261 acttacctat ttttttgttg cagatgggat actgtgattt tgccattgct ggtgaagtgg
     4321 tgaatggacc gtatcagtct gcgttgaaaa acgtttcaga acatggtctc acttcaaaga
     4381 tagatgttcg cttagcaaat ggtttgtcag cttttgaaga agcagataat attgatacga
     4441 tagctatctg tggaatgggt ggtcgtttga ttgctgatat tttgaataac gatatagaca
     4501 aacttcagca cgttaaaaca ctagtcttac aacctaataa tcgggaagat gatttaagaa
     4561 aatggttagc tgcaaatgat tttgaaatag tagcagaaga tatcttgact gaaaatgaca
     4621 aatgctatga aattttagtt gttaagcatg gtcatatgaa tttaactgct aaagaattgc
     4681 gatttggtcc atttttatta tccaataaca caacagtttt taaagaaaaa tggcaaaatg
     4741 agcttaataa attaactttt gctttaaact ctattcccaa tagtaaaatg gaagaacgtg
     4801 ctattttgga agataaaata caggatataa aggaggttct tgatgaaagc tagagaactt
     4861 attgatgtat atgaaactta ttgtccacag gaattatcta tggaaggaga tatttcaggt
     4921 cttcaaattg gatctttaga taaagaaata aagacagtca tggtagcttt agatgttcga
     4981 gagactacag tagctgaagc aattgaacgt caagtagatc ttcttattgt aaagcatgct
     5041 cctatttttc gtccattaaa ggatttggta gcaacaccgc aaaataagat ttatatagac
     5101 cttcttaaat cagacattgc tgtttatgtg agccatacta atatagatat cgttcctaat
     5161 ggacttaacg actggttttg tgaattatta gatattcaat atcccgatat attatctgag
     5221 acaagtaatg gttatggtat tggtcgcatt ggtgatgtta gaccccagag ttttgaattt
     5281 tttgcatgga aaattaaaga tgtgtttggt ttagatagcg ttcgtttagt ttcatatgat
     5341 aaatctaatc ctgaaattca acgagttgct atttgtgggg gtagtgggca aagtttctac
     5401 aaagaagcta ttgcaaaagg agcagatgtt tttgtaacgg gagatattta ttatcatact
     5461 gcacaagaaa tgatcacaaa tggtttgctt gcgattgacc caggccatca tattgaggtt
     5521 ttatttgttt ctaaaatagc tactatgatt gagcagtgga aacttgaaaa aggctgggat
     5581 atttcagttt tagagagtaa agctccaaca aatccctttt accatatgta ggagctatta
     5641 tgaaaaagat agcaattatt ggtgcaggag cagtcggagc gacactagcc tattacttat
     5701 caaaagaaaa ggatattcag gttactgttt ttgattatgg agttggacaa gcaactaagg
     5761 ctgctgcggg gattattagc ccatggtttt caaaacgtcg taataaagcc tggtatcgca
     5821 tggcgaggtt gggggctgat ttttattcaa agctagtcac ggatttacaa aaagatggtt
     5881 ttgaaacaaa attttatcag caaactggtg ttttcttatt aaaaaaggac gagagtcagt
     5941 tagagtcatt gtttgcacta gcagataaac gtcgactaga atctcctcta ataggtgatt
     6001 tacaaatact aaataaaagt gaagcaaata ctcactttcc tgagttagat ggctatgaac
     6061 agttacttta tgcgagtgga ggagcgcgag tagaaggggc ggacttaaca cgaatattgt
     6121 tagaagcaag tggtgttaat gtcatcaagg atgaagttca ctttacgata actgataatg
     6181 gctttcgtgt tcaaggaatt gattttgata aacttgtttt agcgagtgga gcctggctag
     6241 ctaagatatt agatgagcac aactatcagg tagatattcg ccctcaaaaa gggcaattgc
     6301 gagactatta ttttagcaat attaatacag gaaaatatcc tgttgtaatg ccagaaggcg
     6361 agctagatat tatcccattt gataatggta aagtctctgt aggtgcaagt cacgaaaatg
     6421 atatggcttt tgatttaaac attgatttta aagtcttaga caagtttgaa gagcaagcta
     6481 ttggctattt tccgcaatta aaaaaagcag atacgacttc tgaacgtgtt ggaattcgtg
     6541 cctatacaag tgatttttct ccattttttg gccctgtccc ttgtatggag ggtgcatatg
     6601 ctgcaagtgg attaggatca actggcttaa cagtgggccc attaattggt tatgaattat
     6661 gtcaattaat cttaaataaa gaaaatcagc taaatttaga ggattacgat attacgaaat
     6721 acgtgaaaaa gattagtaga tgatagcgtt taaaaaaagt gttataatat gtaggattaa
     6781 tataaataag gagaccttac tatgaaaggt attattttag cgggaggttc aggtactcgc
     6841 ttgtacccat taactcgtgc tgcatcaaaa caattgatgc caatttatga taaaccaatg
     6901 atttattatc cattgtcagt tttgatgttg gcaggaataa aagaaatact gattatttct
     6961 actccacaag atttgcctcg ctttgaagat atgcttggag atggttcaga actaggaatc
     7021 tctctttctt atgctgaaca accaagccca gatgggttag cacaggcctt cattatcggt
     7081 gaagaattta ttggcgatga tcacgttgcc ttagtattag gtgataatat ttaccatgga
     7141 ccaggtttat ctgcaatgct acaacgtgca gctagtaaag aaagtggagc aaccgtcttt
     7201 ggctatcaag taaaagatcc agaacgtttt ggtgttgtcg agtttgatac tgatatgaat
     7261 gccatttcta ttgaagaaaa accagcgcaa cctaagtcta attatgctgt cacaggtctt
     7321 tatttctatg ataacgatgt ggttgaaata gctaaaaata ttaagccaag tcctcgtgga
     7381 gaacttgaaa ttactgatgt taacaaagcc taccttgata gaggtgattt atcagttgag
     7441 ttaatgggac gtggatttgc gtggttagac actggaactc acgaaagtct tcttgaagca
     7501 gcacaatata ttgaaacagt acaacgcatg caaaacgttc aagttgctaa ccttgaggaa
     7561 attgcttacc gcatgggcta catcactcgt gaacaagtcc ttgagttggc acaaccactt
     7621 aagaaaaatg agtatggtca atacctactt cgtttgattg gagaagccta atgactgaac
     7681 aattttttga taaagaatta acttgtcgcc caattgaagc cattcctgga ttgttggaat
     7741 tcgatattac cgttcgtgga gacaaccgtg gatggttcaa agagaacttt caaaaagaaa
     7801 aaatgatacc gcttggtttc ccagaaagct tctttgaggc agacaaacta caaaataata
     7861 tttcgtttaa caaaaaaaat actttgcgag gtctccatgc agagccttgg gataaatatg
     7921 tttcgatcgc tgatgaagga cgtgtgatcg gtacttgggt tgacctccgt gaaggtgaca
     7981 gttttggtaa cgtttaccaa acgattatcg atgcctcaaa aggtattttt gttccacgcg
     8041 gcgttgctaa tggtttccaa gttctttcag ataaagcagc ttatacttat ctcgttaacg
     8101 attattgggc acttgaactc aaaccaaaat atgctttcgt taactatgca gatccaaatc
     8161 taggcattca gtgggaaaac cttgaagaag cagaagtctc agaagcagat aagaatcacc
     8221 cacttctcaa agatgtaaaa cctttgaaga aggaagattt gtaattttca acttcttaaa
     8281 aggcattaac cttagcaagt aaaaacgaat tagcaatgtt gtttgacgca agggtgaaag
     8341 gaaacatgca aaaggcctgt tccctcaggg atttgaaata ttgatttctt aggcatgaca
     8401 ttccatagaa ctacttctaa cagaacagta tccttcaaac agcaaaaaga aaaaattttt
     8461 cggagaaaaa atgactgaat ttaaaaacat tatcgtaact ggtggagctg gtttcatcgg
     8521 atcaaacttt gtacactatg tttacaataa ccacccagat gttcatgtaa ctgtccttga
     8581 taaattgaca tatgcaggta accgtgctaa cattgaagcc attcttggtg atcgtgttga
     8641 gttagttgtt ggtgacatcg ctgacgctga attggtagac aaattggctg ccaaagcgga
     8701 tgctattgtt cactatgcgg ctgagagtca caacgataac tcattggaag atccaagtcc
     8761 atttatccat acaaacttta tcggaactta cactttgctt gaagcagctc gtaaatacga
     8821 tatccgtttc caccacgtgt caactgatga agtttatgga gatcttccac ttcgtgaaga
     8881 ccttccagga aatggtgaag gaccaggtga aaaattcact gctgaaacaa aatacaatcc
     8941 atcatcacct tactcatcaa ctaaggcagc ttctgacctt atcgttaagg catgggtacg
     9001 ttccttcggc gtaaaagcga ccatttcgaa ctgttcaaat aactatggac cataccaaca
     9061 catcgaaaaa ttcattccac gtcaaattac aaatatcttg gcaggaatca aaccaaaact
     9121 ttatggtgaa ggtaaaaacg tccgtgactg gatccacaca aatgaccatt caacaggtgt
     9181 ctgggctatt ttgactaagg gtcgtattgg tgaaacatac cttattggtg ccgacggtga
     9241 gaaaaacaac aaggaggttc ttgaacttat ccttgagaaa atgggtcaac caaaagacgc
     9301 ttacgatcac gtaactgacc gtgctggtca tgatcttcgt tacgctatcg attctacaaa
     9361 attgcgtgaa gaactcggct gggaaccaca atttacaaac ttttcagaag gtttggaaga
     9421 aactattaaa tggtatacag aaaatgagac atggtggaaa gcagaaaaag atgctgtaga
     9481 agccaagtat gctaaaactc aagaagtcat taagtaattg aaccaaaaaa cctatcattt
     9541 tgatgatagg ttttttatgt acggctagtt agatagctaa ttggtctgtt tttgtcatgg
     9601 tttgaggttg gtaatagaca ttttgatagt cattaccaac tttttgtact aggtaaagtc
     9661 ccgctttatt cattttgaat tgattgttaa tgagactctc ttgattatcg tgtttgataa
     9721 cagcccaagt ttgactgttt ttatcataga taacttgttg ttttgaacta ttttctagta
     9781 attctacttc tttgctgttg gctaatttat caaaacttgt gcgatcaatg tttggaacca
     9841 tcatgtaacc atagctatca cctttgttgt catgcttttg gcttatagtg ataaaaggat
     9901 tgctaacgat tgaggtattt ttagaagtac gattaatgct gttccaagta cctgtttgct
     9961 ctttgcgttc aatatcaata gtgctatttt taaagaagat ataaccaata ttgcgaccag
    10021 gttcttttga ttctaaaaag acactttttg tatctgtaaa ttgttgagaa cttgcttgtt
    10081 ttaaatcaat agtttttcca ttgacgtatg tagtataagg tgttttagaa tcgtcttttc
    10141 gttgatcaat tgttgtagaa acatttccaa tgccgttagt attcttgatg ttgctaccta
    10201 aaaagacaat cttatcattt aagataaccc aacctttttg tgctgttaag gtgcgatccc
    10261 agttagtaaa atccatagcg gcaagagcaa agtgatcatt aagtttgacg gaaccaacaa
    10321 agtcagatgt tcctgtaact tgaccggttt tttctttagc gtctttgcta tgtttgctca
    10381 tgaattcctt agtggtatct tcacgcttag catctttttc agttgttcca gccattttat
    10441 aaggattgac ggttggccaa aaatgattac tataatgaga ttgatcacta ttataaatat
    10501 agaacatacc atctccggta taccaaccac gtgtattttc atcattcatt ccttcatagt
    10561 tgagggtacg tttagaatgt aatgaaagcg cgaaaccaaa gtctttcttg gcattataat
    10621 aagctaagcg gtccatgcta ttaaaggttg ataaattact ttttaaaggt ttagtagcga
    10681 ctgtactgtc attaagcagc ttattcatgt tggcaatatc ggaatacgat ttgaggttat
    10741 tgaagacatt gtagaattta tttgaagtga taatcgtttt aatagttgat ttgaggtcta
    10801 agtttcgctc ttcattagac atgttagcca atctgaggaa acctctgaga acttcaactg
    10861 cagccgcatg cgaagaagct gcctctctac taattgagcg tccacgactc atatccatta
    10921 actcaccttt tacaattaaa ggtaaaaatg attgattaat ccatttataa accatatcaa
    10981 gttcttgatt agagatttta tagtcagttt cttgaatgat aggcagcaat tgtgtcaaac
    11041 catctatcag aacattacca taggcgccag tataagcaac atttgtatgg tcgatgtaag
    11101 aaccgtcagc atagaagcct tcagctttag tagcagtagt aaaaagattt tttagagaat
    11161 gagaagtttt ttcgataata gtattgtctt tacgaagtaa accttcaatg attttaacgc
    11221 gccccatatc tactagatta ccaccaaggg ctttaaatgg attgacaagc gttttacgga
    11281 aatattctgc atcaggaaca aagtgttcaa ttgggtcggt ataagttttt atttcagcgt
    11341 cagtgaagta gttattcatg agagataggg tacctgtaat tgagcgagga acaccgattt
    11401 caaaatccca ccaattggca gagccttcta tatctttatt aacattgtag aagttttgat
    11461 gaagccaagc cagactctct tttacagtac gaatagcttt ttcatttttg taaatagtag
    11521 agtgtggatt ggtgatttgt ttagctaaat cttccaaacg acgataagta gcggttaact
    11581 gtgctgaatt attgagatta tctaaatctt tccaaaggaa agtgcgatta gaatccagtt
    11641 tgatagcttc gatgtttttg gcgttagttt catctaattt ctgattaagc ttttgcatgt
    11701 tcgaatcatt agtatcgtaa acataattac caatagtcac gtcgttccat ttgtcgagga
    11761 gttttgtaaa gttatcttct gttgaagctg taactgataa cggtacttct ttaattattt
    11821 tgccagattt atcagatatt ttaacagttg ttgaaccagt agcgtttgga tacaatattc
    11881 caccaacaat tttcccaaga gacggattgg ttagagtata ttggtagtca gctttattaa
    11941 aaacgtaatt tttgtttaaa gccgtattaa cgcttttttc aatttgtgtt gtgacgggtt
    12001 gtggatgctc tgagtcttta gggccttttg ttttcattga tatattatca aaagtaacaa
    12061 aaccagttcc tttttcataa taaagttcaa gtgtcacctc ggagacattt aactttgggt
    12121 tatatatctt tgttaaggta tgtttgttag tagtaccgct ggtcatctca gaaagccaaa
    12181 gtcgcgtatt gttgtctttt ttctccataa tacgaaggaa agcttgtcca gccttgttac
    12241 ttgtttcaat atcaaactta acctcatatt ttttagtggg atcaatgttc actttttgtg
    12301 ttacagcacc tctaaagcct ttgtcgctgg atagacttac ttggccttgt tcggtacgtt
    12361 ggataatagg tgatttatca gcagtatttc ctttatcaat ccaagcagac cattccctag
    12421 cgcttgtatg tgaccagctt ccagatacag ggttagtttg attaaaatct ccgtttttca
    12481 ccagctcctt attttgagtt tgattatcta ctacttgttt cattgtgcta tgagtggctg
    12541 ttgcagttgc agttgaagct gtggggttag acagatcttg ttgtgtggtt gtggtgctag
    12601 tctccttgac agtggtactt tcagtctgga gaacttcaat attattagtt tgagtatctc
    12661 ttatttggga cgttgaggta gttttggtaa gctcttcagc tttagcttgg taactattgt
    12721 taaccaatat tgttcctaaa ataagggctg aataaagcat aatacgatgt ttctttttga
    12781 tttccatttt tactccttag ggtttaaaat tgtaaaccgg tttttcctaa ttatagcatg
    12841 ctttaaaggt ttatgataaa gaacctttaa aatgtcatca aagtgaaaag taaataactg
    12901 gtaaacaaaa taatttgttt aatcatttgt gtgctacttt gatagtaaat tgcctaaata
    12961 aaggctattt tttgttataa tatgagaatg agaggagttt gaaatgacta aattagcaac
    13021 aatttgttac attgacaatg gcaaggaatt acttctattg catcgcaaca aaaaagagaa
    13081 tgatgttcat gagggaaagt ggatctcggt aggcggtaaa ctggaggcag gtgaaactcc
    13141 ggatgaatgt gctaagcgtg aaattctaga agaaacccat ttaacagtaa aaaaaatgga
    13201 ttttaaagga gtcatcactt ttcctgaatt tacgccaggt catgattggt atacctatgt
    13261 ctttaaagta acagattatg aaggagagtt aatttcagat gatgaatcac gagaaggaac
    13321 cttagaatgg gtaccatatg atcaagttct ctctaagcca acgtggcaag gagattacga
    13381 aatttttaaa tggattttag aagatgttcc tttcttttct gctaagtttg tttatgatga
    13441 acaccaaaat ttaattgaaa agacggttaa tttttatgaa aaatagagat tttagggtta
    13501 gagaccagct cgaaacattc ggagtcagag tttctgcttt aatcatagaa aatcaaaaac
    13561 tgctattaat atatgctcct catctctata aatactattt gccaggtggg gcgcttcaag
    13621 ttggagaaga tagcaataag gctgttgcga gagaagtact tgaggaaata ggtcttcaca
    13681 gccaagttgg tgatttagcc tatattgttg agaatcagtt taatattaag agacatcatt
    13741 atcactcagt tgaattttta tattttgtta atctcttggg tcaggcgcca gaatctatca
    13801 aagaaggaac gcataagcga cattttgtat ggcttccaat aaaagaatta acaaaaattg
    13861 attgtaatcc taatttttta gcacaggatt tgatagaatg gccaggtcat gttgtgcata
    13921 aaatcgttca aaatta
//
LOCUS       DATRQA010000045        13866 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.045, whole genome shotgun sequence.
ACCESSION   DATRQA010000045 DATRQA010000000
VERSION     DATRQA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13866
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.045"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..111)
                     /locus_tag="VBD27_000979"
     CDS             complement(<1..111)
                     /locus_tag="VBD27_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7025004.1"
                     /translation="MSSVLTLTNILFLLQGFGLTLYISFISILLSMFFGTL"
     gene            complement(123..818)
                     /locus_tag="VBD27_000980"
     CDS             complement(123..818)
                     /locus_tag="VBD27_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7025005.1"
                     /translation="MTLLNISPFAISRWGAFFNHFDLFFKGFLYTLGISFGALLLALI
                     LGILSGGLSTSKSKVGKLISRIYVEVFQNTPLLVQMVFVYYGLAIISNGHIMISAFFT
                     AVLCVGLYHGAYISEVIRSGIEAVPKGQTEAALAQGFTANQTMQLIILPQAIRTILPP
                     MTNQVVNLIKNTSTVAIISGADIMFLAKAWAYDTTNYIPAFAGAAIFYFVICFPLASW
                     ARKQEELNKKTYS"
     gene            complement(831..1631)
                     /locus_tag="VBD27_000981"
     CDS             complement(831..1631)
                     /locus_tag="VBD27_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7025006.1"
                     /translation="MLRRKRLTLYLLSCIFIFLLFYPNSTSANQLSEIKKSGVLKVGV
                     KQDVPNFGYYNAETNQYEGMEIDIAKKIAKSLGVKPVFVPTTAQTREPLMDNGQIDIL
                     IATYTITPERKANYNISKAYYHDEIGFLVRKNSHIKTIKELDGKHIGVAQGATTKVNL
                     EKYAKEHKLKFSYAQLGSFPELAISLYANRIDAFSVDKSILSGYLSPHTTILKEGFNT
                     QEYGIATSKQDKVLIPYVNKLLVSWEKDGSLKHIYQKFKLKPAKVKKE"
     gene            complement(1643..2398)
                     /locus_tag="VBD27_000982"
     CDS             complement(1643..2398)
                     /locus_tag="VBD27_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7025007.1"
                     /translation="MSLISYKNVNKYYGDYHALRQINLEIEPGQVVVLLGPSGSGKST
                     LIRTMNALESIDDGSLVVNGHELANISSKELVNLRKEVGMVFQHFNLYPHKTVLENIT
                     LAPIKVLKQSKKEAMEIAEKYLKFVNMWERKDSYPSMLSGGQKQRIAIARGLAMHPKL
                     LLFDEPTSALDPETIGDVLSVMQKLANDGMNMVVVTHEMGFAREVADRIIFMADGEIL
                     VDTTDVQDFFDNPTEPRAKQFLRNIINHTSTDL"
     gene            2738..3448
                     /locus_tag="VBD27_000983"
     CDS             2738..3448
                     /locus_tag="VBD27_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7025008.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYETATAFDGREALVQYAEF
                     QPDLIILDLMLPELDGLEVAKEVRKTSHIPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTENIETAVAEESAQNASSDITIGELQILPDAFIAKKRGEEIE
                     LTHREFELLHHLATHIGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPGR
                     PEYILTRRGVGYYMKSYE"
     gene            3441..4790
                     /gene="vicK"
                     /locus_tag="VBD27_000984"
     CDS             3441..4790
                     /gene="vicK"
                     /locus_tag="VBD27_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEO7025009.1"
                     /translation="MNNSAANIRSFELALLFLLVFVAVYFVYLAVRDFKMSKDIRLLN
                     WKVRDLIAGNYSDSILIQGDADLVELGESLNDLSDVFRMAHDNLEQEKNRLASILTYM
                     TDGVLATDRSGKIVMINETAQQQFNLAYDEALSMNIVDMLGSGSPYSFQDLVSKTPEV
                     VLNRRDENGEFVTLRIRFALNRRESGFISGLVAVSHDATEQEKEERERRLFVSNVSHE
                     LRTPLTSVKSYLEALDEGALNEEVAPSFIKVSLDETNRMMRMISDLLSLSRIDNEVTH
                     LDVEMTNFTAFMTSILNRFDQIRNQKTVTGKVYEIVRDYPLKSIWVEIDTDKMTQVID
                     NILNNAVKYSPDGGKITVNLRTTKTQMILSISDQGLGIPKKDLPLIFDRFYRVDKARS
                     RQQGGTGLGLSIAKEIVKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVTYEEWEDVED"
     gene            4794..5603
                     /locus_tag="VBD27_000985"
     CDS             4794..5603
                     /locus_tag="VBD27_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7025010.1"
                     /translation="MSEIGFKYSILASGSTGNCFYIETPQKRLLIDAGLTGKKVTSLL
                     AEINRKPEDLDAILVTHEHSDHIKGVGVLARKYHLDIYANEQTWKVMDERNMLGKVDV
                     SQKHVFGRGKTLTFGDLDIESFGVSHDAVDPQFYRMMKDDKSFVMLTDTGYVSDRMAS
                     LIENADGYLIESNHDIEILRSGSYPWTLKQRILSDKGHLSNEDGSETMIRTIGNRTKH
                     IYLGHLSKENNIKELAHMTMENNLMRADFGVGTDFSVHDTSPDSATPLTRI"
     gene            5606..5974
                     /locus_tag="VBD27_000986"
     CDS             5606..5974
                     /locus_tag="VBD27_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEO7025011.1"
                     /translation="MKKIFYISLGFISLGIGIAGIVLPVVPTTPLVLLSAFCFSRSSE
                     KFDIWLRQTKVYKYYAADFVESRSIAPARKKSMIWQIYILMGISIYFAPLMWLKLGLL
                     IGTIVGTYVLFYVVPDKIDE"
     gene            6149..6835
                     /locus_tag="VBD27_000987"
     CDS             6149..6835
                     /locus_tag="VBD27_000987"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO7025012.1"
                     /translation="MKELRSKLEKDYGIVFANQELLDTAFTHTSYANEHRLLNISHNE
                     RLEFLGDAVLQLLISQYLFTKYPQKAEGDLSKLRSMIVREESLAGFSRLCGFDHYIKL
                     GKGEEKSGGRNRDTILGDLFEAFLGALLLDKGVEVVHAFVNKVMIPHVEKGTYERVKD
                     YKTSLQELLQSHGDVKIDYQVTNESGPAHAKEFEVTVSVNQENLSQGIGRSKKAAEQD
                     AAKNALATLQ"
     gene            6843..10382
                     /gene="smc"
                     /locus_tag="VBD27_000988"
     CDS             6843..10382
                     /gene="smc"
                     /locus_tag="VBD27_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO7025013.1"
                     /translation="MFLKEIEMQGFKSFADKTKVEFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVSVTLDNSDHFIENIADEVRVER
                     RIFRNGDSEYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQSKLEQTQGNLDRLEDIIYELDMQVQPLEKQASIAKRFLVL
                     DEERQGLHLSILIEDILQHQSDLTTVEEKLLTVREELATYYQQRQSLEDENQSLKQKR
                     HHLSEEIEVKQLALLDVTKLKSDLERQIDLIRLESSQKAEKKEEAGQRLAELEVKAKD
                     CNDQITQKNIELTTLSEKIAQIRSEIVSIESSLERFSTNPDQVIEKLREDFVTLMQEE
                     ADTSNALTALLADIENQKQASQAKSQEIQEVSKNLEVLKSNAKVALERFEAAKKNVRQ
                     LLSHYQDLGQTLQNLEGEYKNQQSILFDHLDEIKSKQARISSLESILKNHSNFYAGVK
                     SVLQAKDQLGGIIGAVSEHLSFDKHYQTALEIALGGSSQHIIVEDESAAKRSIAFLKK
                     NRQGRATFLPLTTIKPRELAQHYLSKLQSSQGFLGIASELVTYDQRLSNIFKNNLGLT
                     AIFDTVDNANVAARQLNYQVRLVTLDGTELRPGGSYSGGANRQNNTVFIKPELDNLKK
                     ELKQAQSKQLIQEKEVAALLEQLKEKQETLAQLKNDGEQARLEEQRADIEYQQLSEKL
                     ADLNKLYNGLQLSSGALEQTTSENEKNRLEKELEQFAIKKEELTTSIAQIKEDKDSIQ
                     EKVNNLTTLLSEAQLEERDLLNEQKFERANCTRLEITLSEIKRDISNLQTLLSHQDSQ
                     LDKEELPRIEKQLLQANKRRENDEEKLVSLRFELEDCEAALDDLAASLAKEGQKNESL
                     IRQQAQLESQCEQLSQQLMIFSRQLSEDYQMTLDEAKVKANVLEDILMAREQLKSLQA
                     KIKALGPVNIDAIAQFEEVHERLTFLNTQRDDLVHAKNLLLETITDMDDEVKTRFKST
                     FEAIRHSFKETFVQMFGGGSANLILTEGDLLSAGVDISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGT
                     MSAADSIYGVTMQESGVSKIVSVKLKEAQEMTN"
     gene            10473..11270
                     /locus_tag="VBD27_000989"
     CDS             10473..11270
                     /locus_tag="VBD27_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7025014.1"
                     /translation="MIKLVATDMDGTFLDENGTYDKKRLANVLKKFKEQGIVFTAASG
                     RSLLSLEQLFADFRDQMAFIAENGSAAVLFNRLAYEQHLSREQYLDIIDHLSKSPYME
                     NNEYVLSGKDGAYILSDANPDYIEFITHYYDNLQKVSHFEDVDDIVFKVTANFTEETV
                     RQAEEWVNQAIPYATAVTTGFKSIDIILSSVNKRNGLEHLCEQYGIRAEEVLSFGDNI
                     NDLEMLEWSGKAIATENARPEVKEIADCIIGHHNDQAVMAYLESMVD"
     gene            11270..12094
                     /locus_tag="VBD27_000990"
     CDS             11270..12094
                     /locus_tag="VBD27_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7025015.1"
                     /translation="MLDIKILALDLDGTLFTTDKKVSEENKVALKAAREKGIKVVITT
                     GRPLKAIGNLLEDLELVSDEDYSITFNGGLVQQNTGKILAKTAMTRQEVEDIHEELYQ
                     VGLPTDILSEGTVYSIANKGHHSQYHLANPLLEFIEVDDLEQVPKDVVYNKIVSVIDA
                     TYLDQQIAKLPDRLKVDYEMFKSRDIILELMPKGVHKAVGLELLTKHLGLDSSQVMAM
                     GDEANDLSMLEWAGLGVAMANGIPEAKATAKATTICNNDESGVAEAVGKYILSEEN"
     gene            12094..13704
                     /gene="ftsY"
                     /locus_tag="VBD27_000991"
     CDS             12094..13704
                     /gene="ftsY"
                     /locus_tag="VBD27_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO7025016.1"
                     /translation="MGLFDRLFGHKKKDKELEIEASESVVLENEDSVIDKEESSNFSK
                     ESTLNRTSEVPVAEDDSFLELERDTALSESHQPVTSEIHPLESEDTSEIPVKEDDSFL
                     ELEDRAKTKVADTSEVGNVVPDSTTLSDNVSAKSEASFSDEEQSSDSQASEQFSETPL
                     QEEMSSGKTEVQTESEDTSAADAFLADYYAKRKAIEKEISSNSLSTDESELSEAQEVL
                     SQSQADTIKAESQEEKYNRSLKKTRTGFSARLNAFLSNFRRVDEEFFEELEEMLILSD
                     VGVNVATQLTEDLRYEAKLENAKKSEDLKRVIVEKLVEIYEKDGIYNEAINFQEGLTV
                     MLFVGVNGVGKTTSIGKLAHQYKSQGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVVT
                     GEKKADPASVVFDGMEKAVAQGVDVLLIDTAGRLQNKENLMAELEKIGRIIKRVVPDA
                     PHETLLALDASTGQNALSQAKEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVK
                     FIGFGEKIDDIGEFNSEDFMRGLLEGIL"
BASE COUNT         4668 a         2145 c         2842 g         4211 t
ORIGIN      
        1 tagagttcca aaaaacattg atagtaaaat ggaaataaaa gatatatata aggttaaacc
       61 gaacccttga aggagaaata gtatatttgt tagtgttaag actgatgaca tatcaaacct
      121 ccttaagaat aagttttctt atttaattct tcttgttttc ttgcccagct tgctagtggg
      181 aaacaaataa caaaatagaa gatagcagct cctgcaaaag ctgggatata attagtcgta
      241 tcataagccc aggccttagc taaaaacatg atatcggctc cagaaatgat tgctacggta
      301 gaggtattct tgattaggtt aacaacttgg ttggtcattg gtggtaaaat tgttctgata
      361 gcttgaggca aaatgataag ttgcatggtt tgattagctg tgaatccttg agctaaagca
      421 gcctctgttt gtcctttagg gacagcttca attcctgagc gaataacttc agaaatatag
      481 gcaccatggt ataggccaac gcacaaaaca gcggtgaaaa aagcactaat cataatatga
      541 ccattactaa tgattgctag tccatagtag acaaaaacca tttgaaccaa aagaggtgtg
      601 ttttgaaaaa cttcaacata gatacgtgat atcaacttac caactttact tttagaagtt
      661 gataaaccac cagagagaat tcctaatatc agagcaagta ggagtgcacc aaacgaaata
      721 cctagagtat ataagaagcc tttaaagaat aggtcaaaat ggttaaagaa agcgccccac
      781 cttgaaatgg caaaaggact gatgtttaat aaagtcataa aaaatcctcc ctattctttt
      841 tttactttag cgggtttaag cttaaatttt tgataaatgt gttttaaact accatctttt
      901 tcccatgata ccagtaactt attaacgtag ggaatcaaga ctttgtcctg tttactggtt
      961 gcaataccgt attcttgcgt attgaaccct tcttttaata tggttgtatg agggctaagg
     1021 tagccgctaa ggatagattt atcaactgaa aaagcatcaa tacgattagc gtacagcgat
     1081 atcgctaatt ctgggaaact ccctaattga gcatagctaa attttagctt gtgttcttta
     1141 gcatattttt ctaaattgac ttttgtagtg gcaccttgag ccacaccaat gtgtttacca
     1201 tccaattctt ttatagtttt gatatgacta tttttacgca ccaaaaatcc aatttcatcg
     1261 tgataataag ctttagaaat attatagtta gcttttcttt cgggcgtgat agtgtatgtt
     1321 gcgattagaa tatcaatttg cccattgtcc atcagaggct ctctggtttg tgcagttgtc
     1381 ggtacaaaaa ctggtttgac gcctaaggat ttggcaattt ttttggcaat atcaatttcc
     1441 attccttcgt attgattcgt ctcagcatta taataaccaa agttaggcac atcttgcttg
     1501 actccaactt taaggacacc cgattttttg atttctgata attgattagc tgacgttgag
     1561 ttgggataaa ataacaggaa aatgaaaata caggataata gatagagcgt taatcttttt
     1621 cgtctcagca tatttcgtcc tcctaaaggt cagtactggt atggttgata atgtttctta
     1681 aaaattgttt ggcacgtggt tctgtgggat tatcaaaaaa gtcttgcaca tccgtagtat
     1741 ctactaaaat ttccccgtca gccataaaaa taatacgatc agcaacttct cgtgcgaatc
     1801 ccatttcatg agttacaacg accatattca ttccgtcatt tgccaatttt tgcatcactg
     1861 aaagaacgtc tccgatggtt tcgggatcaa gggcagaggt aggctcatca aagaggagga
     1921 gttttggatg catggcaagc cctctagcaa tagcaattct ttgtttttga ccacctgata
     1981 gcatagatgg gtaggaatct tttctttccc acatattgac aaattttaaa tatttttcag
     2041 caatttccat agcttctttt ttagattgtt ttaatacttt aataggagct aatgtaatat
     2101 tttctaacac ggttttgtgg ggataaaggt taaaatgttg aaaaaccata ccaacttctt
     2161 tacggaggtt gaccaattct ttagatgaaa tatttgctaa ttcatgtcca ttcacgacca
     2221 aactaccatc gtcaatagat tcgagagcgt tcattgttct aatgagtgtt gatttacctg
     2281 atccagaagg tcctaataga acaaccactt gccctggctc tatttccaaa ttaatctgac
     2341 gaagggcgtg ataatctcca taatacttgt taacattctt ataagatatg agtgacataa
     2401 gaacctccta aaataacagt ttattgttag gttacctaac attatagata agtgtatcac
     2461 ctgtttgtga gcttgtcaat aaattcagaa tattaaagag taggattgat ttaaacattg
     2521 ctatttttag agcggtattt ctagtaaaaa tattacgttc tcgtatcatt tatttttcag
     2581 tttaatagga tgtattgaaa gtttccaatt aactaggaaa tctgttataa ttagtgataa
     2641 tagtggtgct ttaggcccat attatcatat tttcggtcac ttttattttt cagtttatgg
     2701 atggaagtgg gagaccagaa agtaatagag gtaaaatatg aaaaaaatac ttatcgttga
     2761 tgatgaaaaa ccaatttcag atattatcaa atttaattta accaaagaag gatatgaaac
     2821 ggcgacagct ttcgatggcc gtgaagcttt agtacaatat gcagaatttc aaccagattt
     2881 aatcattttg gatttaatgc tccctgaact agatggttta gaagtggcta aggaagttcg
     2941 taaaaccagc catattccta ttattatgct ttccgcaaaa gacagtgagt ttgacaaagt
     3001 cattggttta gaaatcggtg cagatgatta tgttactaaa cctttttcaa atcgtgaact
     3061 tttggcacgg gtcaaagcac atttacgacg tactgaaaat attgaaacag ctgtagcaga
     3121 agaaagtgct caaaatgctt cttcagatat tactattggt gagttgcaaa ttttaccaga
     3181 cgcctttatt gctaagaaac gtggcgaaga aattgaatta acgcaccgag aatttgaatt
     3241 acttcaccat ttagcgacac atataggaca agtcatgaca cgcgaacatt tattggaaac
     3301 ggtttgggga tatgattatt ttggagatgt ccgtacagtt gatgtgacgg tgagacgttt
     3361 acgtgaaaag attgaagata ctcctggtcg tccagaatat attttgactc gacgtggtgt
     3421 aggttattac atgaagtctt atgaataata gtgctgcaaa tattaggtcg tttgagctag
     3481 ccttactttt tttactagtt tttgttgctg tctattttgt ttatctagcg gttagagatt
     3541 ttaaaatgtc taaagatatc cggcttttga attggaaagt cagagatttg atagcaggaa
     3601 attattcaga tagtattcta attcaaggtg atgcagattt ggtagaatta ggggaaagtc
     3661 taaatgattt atctgatgtc ttccgaatgg cacatgataa cttagaacaa gaaaagaatc
     3721 gcttagcgag tatcttaact tatatgactg acggtgtgct ggctacagat cgttcaggaa
     3781 aaatcgttat gattaatgag acagcccaac agcagttcaa tctagcatat gatgaagctt
     3841 tatcaatgaa tattgttgat atgttagggt cgggaagtcc atattcgttc caagatttag
     3901 tctctaagac gccagaggta gttcttaata gacgtgatga aaatggtgaa tttgttacct
     3961 tacggattcg atttgctttg aaccgtcgtg aaagtggttt tatttccggt ttagttgcag
     4021 tatcacacga tgctactgag caagagaaag aagagcgtga aagaagactt tttgtatcta
     4081 atgtttctca tgaacttcgt acacctttga catctgtaaa atcttattta gaagctttag
     4141 atgagggagc gctcaatgaa gaagttgctc caagcttcat taaggtatct ctagatgaaa
     4201 caaatcgtat gatgcgtatg atatcagatt tattaagttt atcgcgcatt gataatgaag
     4261 taacgcattt agatgttgaa atgacgaatt ttacagcttt catgacctca attttgaatc
     4321 gatttgatca gattagaaat caaaaaacag tcacaggaaa agtttatgaa attgtcagag
     4381 attatcctct taagtcaatt tgggtggaaa ttgatacaga taagatgact caagtgattg
     4441 ataacatttt aaataatgca gtcaagtatt caccagatgg tggtaagatt acagttaatc
     4501 tacgcacaac taaaacgcag atgattttat caatatcaga ccaaggctta ggtattccca
     4561 aaaaagattt acctctcatt tttgatcgtt tttatcgtgt tgataaggcg agaagtcgtc
     4621 aacagggtgg gactggactt ggtttgtcaa ttgcaaaaga aattgttaag cagcataagg
     4681 gatttatttg ggctaagagt gagtatggta aagggtctac ttttacaatc gtcttgcctt
     4741 atgataaaga tgctgtaact tatgaagaat gggaggacgt tgaagattaa aacatgtcag
     4801 aaataggatt taaatatagt atcttagctt caggttcaac tggaaattgt ttttatattg
     4861 agacacccca aaaacgtctt ttaattgacg ctggtttaac aggcaagaaa gtgactagcc
     4921 ttcttgcaga aattaatcgt aaaccagaag atttagatgc tatactagtt acccatgaac
     4981 attctgacca cattaaaggt gttggcgtat tggctaggaa atatcacctt gatatttatg
     5041 ccaatgagca gacgtggaaa gttatggatg agcgtaacat gttaggcaaa gttgatgtta
     5101 gtcaaaagca tgtttttgga cgcggtaaaa ccttaacttt cggggattta gatattgaga
     5161 gctttggtgt aagtcatgat gctgtggatc cccaatttta ccgtatgatg aaagatgata
     5221 agagctttgt gatgttgaca gatacaggat atgttagcga ccgaatggca agcttgattg
     5281 agaatgcaga tggttatttg atcgagtcaa atcacgacat tgagattcta cgttcgggtt
     5341 cttatccttg gacacttaag caacgtattt tatcagataa aggacattta tctaatgaag
     5401 atggttcaga aaccatgatt cgcactatag gtaaccgtac taaacacatc tatttaggac
     5461 acttgagtaa ggaaaataat attaaggaac ttgctcacat gactatggaa aacaatttaa
     5521 tgagagctga ctttggtgtg ggcacagatt tttctgttca cgatacttca cctgattcag
     5581 ctaccccact gacgaggatt taagattgaa aaagattttt tacattagtt taggatttat
     5641 tagtttaggg ataggcattg caggtattgt cttgccagta gttccaacga ctccattagt
     5701 attgctttca gctttttgct tttcgagaag ctctgaaaaa tttgatattt ggttgcgtca
     5761 aactaaagtt tataaatact acgctgcaga ttttgttgaa agcagaagta ttgccccagc
     5821 gcgtaaaaag tcaatgattt ggcaaattta tatcttgatg gggatttcaa tatattttgc
     5881 tcctctaatg tggctcaaac tgggattatt gattggaact attgtaggaa cttatgtgct
     5941 tttttatgta gtacctgata aaattgatga atagttaaca agagactagg tgtgtatcct
     6001 agtcttttgt tgaatattca aatatgtaat taaggaaacg ttttctttgg aagataagtt
     6061 taacttccaa aatgtttgat tatttgtcaa ctgtggtata atggtatttg tttaatattt
     6121 tatctatgat tgaaatcaag gagaaaaaat gaaagaatta agatcaaagc tggaaaaaga
     6181 ctatggtatt gtttttgcta atcaagaatt attagataca gcttttaccc atacatcata
     6241 cgcaaatgag catcgcctcc taaacatttc acataatgaa cgtttggaat ttttaggaga
     6301 cgctgttcta caattactta tttcacagta tctttttaca aagtaccctc aaaaggcaga
     6361 aggtgattta tcaaaattac gttcaatgat tgttcgtgag gaaagtcttg caggtttttc
     6421 acgtctatgt ggctttgatc actatattaa acttggaaaa ggagaagaaa aatctggtgg
     6481 acgtaatcgt gacactattt taggagattt atttgaggct tttttaggag ctttattatt
     6541 ggataaaggt gttgaagtgg ttcatgcctt tgttaacaag gtaatgatcc ctcacgttga
     6601 aaaagggacc tatgagcgtg ttaaagatta caagacaagc ttgcaagaat tacttcaatc
     6661 tcatggggat gtaaaaatag attatcaagt tactaatgaa agtggtccag cacatgcaaa
     6721 ggaatttgag gtgactgtat ctgtcaacca agaaaatttg agccaaggaa ttggacgatc
     6781 aaagaaagct gctgaacaag acgctgccaa aaatgcttta gcaacgttac aatgaggaaa
     6841 tcatgttttt aaaagaaatt gaaatgcaag gctttaagtc ctttgcggat aaaactaagg
     6901 tagaatttga tcaaggagtt actgctgttg taggtccaaa tggatcaggt aaaagtaata
     6961 tcacagagag tttacgttgg gcattgggag aatcaagcgc aaaaagttta cgaggaggta
     7021 agatgccaga cgtcatattt gctggtacag aaaatcgtaa gccccttaat tatgcgcaag
     7081 tgagtgttac tttagataac agcgaccact ttatcgaaaa tatagctgac gaagttcgag
     7141 ttgaacgtcg tatttttcga aacggagata gtgaatattt aatagatgga cgcaaagttc
     7201 gcttacgtga tattcatgat ttatttatgg atactggatt agggagagat tctttttcta
     7261 ttatttccca aggacgcgtt gaagctattt ttaatagtaa accagaagag cgccgtgcca
     7321 tatttgaaga agccgctggt gttctgaaat acaaaacacg taagaaagaa acacaatcca
     7381 aattagaaca aactcaaggg aacttagatc gcttagagga tattatttat gaattggaca
     7441 tgcaagtcca accattagaa aaacaagcaa gtattgctaa gcgtttttta gtattagatg
     7501 aggaacgaca aggactccac ttatctattt tgattgaaga tattttacaa catcaatcag
     7561 atttaactac tgttgaggaa aagctattga cagtaagaga agagttagca acttattacc
     7621 aacaacgtca gtcccttgaa gatgaaaatc aatcgttaaa acagaaacgt caccatttat
     7681 ctgaagaaat tgaggttaaa cagttagctc ttttagacgt tacaaagtta aagtcagatt
     7741 tggaacgcca aattgatctg attcgtttag agtcaagcca aaaggctgaa aagaaagaag
     7801 aggcaggtca acgcttagca gagttagaag ttaaagctaa agattgtaat gaccagatta
     7861 cccaaaaaaa tatagagtta actactctgt cggaaaaaat agcccaaata agatcagaaa
     7921 ttgttagtat agaaagttcc ttagagcgtt tttcaactaa tccggatcag gttattgaaa
     7981 aacttcgaga agactttgtt accttgatgc aagaagaagc cgacacttca aacgctttaa
     8041 cagctttact agctgatata gagaatcaaa aacaagcttc tcaagctaaa tctcaagaaa
     8101 tacaagaagt tagcaaaaat ttagaagtat taaaaagtaa cgcaaaggta gcattggaac
     8161 gatttgaggc tgcaaagaag aatgttcgtc agttattatc tcactaccaa gatttaggtc
     8221 aaaccttgca aaatctagaa ggtgagtata agaatcagca gtcaatttta tttgatcatt
     8281 tagatgaaat caaatcaaag caagcacgta tttcgagttt agaatctatt ttgaaaaatc
     8341 atagtaattt ctatgctgga gtgaagtcag ttctacaggc taaggaccag cttggaggta
     8401 tcataggtgc tgttagtgag catcttagct ttgacaaaca ttaccaaaca gcattggaga
     8461 tagctttggg aggtagcagc caacacatca ttgttgaaga cgagagtgct gccaaacgtt
     8521 caattgcttt tttaaagaaa aaccgccaag gtagagcgac atttctgcct ttaactacaa
     8581 taaaaccaag agaattagca caacattatc tctcaaaatt acaaagttct caagggtttt
     8641 taggaatagc tagtgaattg gtaacctatg atcaacgctt gtcaaacatt tttaaaaata
     8701 acttagggtt aactgctatt tttgatacgg ttgataatgc taatgtagct gcaagacaat
     8761 tgaattatca agtacgttta gtaaccttag atggaacaga gttacgccct ggagggtcgt
     8821 actcaggggg agcaaatcgt caaaataata ctgtttttat caagcctgaa ttggataatc
     8881 taaaaaaaga attgaaacag gcacaaagta aacagttaat tcaagaaaaa gaagttgccg
     8941 cattgttaga gcaactcaaa gagaaacaag aaaccttagc acaacttaag aatgatggag
     9001 aacaagctcg tcttgaggaa caaagagctg atatagagta tcaacaacta tcagaaaagc
     9061 ttgctgattt aaataaacta tataatggct tgcaattaag cagcggggcg ttggaacaaa
     9121 ctacttctga aaatgaaaaa aatcgtttag aaaaagaact tgaacaattt gctattaaaa
     9181 aagaagagtt gacaacttca attgctcaaa ttaaggaaga taaagattca attcaagaaa
     9241 aagtcaataa tctaacaaca ttattgtcag aggcacagct agaagaacgt gatttgctaa
     9301 acgagcagaa atttgaacgt gccaactgta cacgtctaga gattaccttg tctgaaataa
     9361 aacgtgacat tagtaattta caaacgttac taagccatca ggacagtcaa ttagacaaag
     9421 aagagcttcc acgtatagaa aaacagttgc tacaagcaaa taagagaaga gaaaatgacg
     9481 aagaaaagct tgtgagtctc cgttttgaat tggaggattg tgaagctgct ttagatgatt
     9541 tagctgcttc tctggctaaa gaaggacaaa aaaatgaaag ccttattcgt cagcaagctc
     9601 aattagagtc acaatgtgaa caattatctc aacaattgat gatattctca cgtcaattat
     9661 cagaagacta tcagatgaca ttggatgaag cgaaggtaaa agcaaatgtc ttggaagata
     9721 ttttgatggc gcgtgaacaa ttgaaatctc tacaagctaa aattaaagct ttaggacctg
     9781 ttaatattga tgcaattgct caatttgaag aagttcatga acgattgacc ttccttaaca
     9841 cacagcgaga cgacttagtt catgccaaaa atttattact agagacgatt acagatatgg
     9901 atgatgaagt taagactcgt tttaaatcga catttgaagc cattcgtcat agtttcaaag
     9961 aaacctttgt acagatgttt ggtggaggtt ctgcgaacct tattctgaca gaaggtgatt
    10021 tattatcagc tggggttgat atttcggttc aacctcctgg taaaaaaatc cagtctttaa
    10081 acttgatgtc aggtggtgaa aaagcattat cagcgttagc attattattt gccattatcc
    10141 gtgttaagac aattccattt gtcatcttgg atgaagttga ggccgcttta gatgaagcaa
    10201 atgttaagcg atttggcgat tatcttaatc gatttgataa atcaagtcaa tttatcgttg
    10261 ttactcaccg aaaagggact atgtctgctg ctgatagtat ctatggggta acgatgcagg
    10321 aatcaggcgt ttccaaaatt gtttctgtaa aattaaaaga agctcaagaa atgaccaatt
    10381 agaaaacaga aggggatccc ttctgttttt tggattttca gccttatttt atgttacaat
    10441 agtaaatgta aaccctttag ttaggagatt ccatgataaa attagtagca accgatatgg
    10501 acggtacatt cttagatgaa aacggtacct atgataaaaa acgtttagca aatgtgctaa
    10561 aaaaatttaa agaacaagga attgtcttta cggcagcaag tgggcgttcc ttattatcac
    10621 ttgaacaatt atttgctgac tttcgtgatc agatggcttt tatagcagaa aacggaagtg
    10681 cagcagtctt atttaatcgc ttggcatatg aacagcactt gagtcgagaa caatacttag
    10741 atattattga ccatttgagt aagagccctt atatggagaa taatgaatat gtcttatctg
    10801 gcaaggatgg tgcatatatt ctaagtgatg ccaatccaga ttatattgaa ttcatcactc
    10861 attattatga taatttgcaa aaagtttctc actttgaaga tgttgatgat attgttttca
    10921 aagtaacggc taactttact gaagagactg ttagacaagc tgaggagtgg gtaaatcaag
    10981 ctataccgta tgccacagca gtgacaacag gttttaaatc cattgatatt attctaagct
    11041 cagttaataa acgaaatggt ttagagcacc tctgtgaaca atacggtatt agagcagaag
    11101 aagtcttatc ttttggtgac aatattaatg atttagaaat gctagagtgg tcaggaaaag
    11161 ctatcgcaac agaaaatgct agaccagaag ttaaagagat agcagattgt attataggtc
    11221 atcacaatga tcaagctgta atggcttatt tagaaagtat ggtggattaa tgttggatat
    11281 taaaatactt gccttagatt tagatggcac tttatttaca acagataaga aagtttcaga
    11341 agaaaataaa gtggccttaa aagcagctag agaaaaaggt ataaaagttg ttattacaac
    11401 agggcgtcct ttaaaagcaa ttggtaattt gttagaggac ctagagttag tatctgatga
    11461 agattacagc attactttta acggtggttt agttcaacaa aatacaggga aaatcttagc
    11521 taaaactgca atgacacgac aagaagtgga agatatccat gaggagttgt atcaagtggg
    11581 tttgccaaca gatatcttaa gtgagggaac ggtatatagt attgctaaca agggacacca
    11641 ttctcaatat catttagcca acccactctt agaatttata gaagttgatg atttagagca
    11701 agtgccaaag gatgtagttt ataataaaat tgttagtgtt attgatgcta catatcttga
    11761 tcaacaaatt gctaaacttc ctgatagact taaagttgac tatgaaatgt ttaaatcacg
    11821 tgatatcatt ttagaattga tgcctaaagg tgttcataaa gcagtcggct tagaactact
    11881 gacaaaacac cttgggttag atagtagtca agttatggca atgggagatg aagcaaatga
    11941 tttatcaatg ctagagtggg caggcttagg agttgcaatg gctaatggta ttccagaagc
    12001 taaggcaaca gcaaaagcga caactatttg taataacgat gagtctggag ttgccgaagc
    12061 agttgggaaa tatattttaa gtgaggaaaa ttaatgggat tatttgaccg cctatttgga
    12121 cataagaaaa aagataaaga actagaaatt gaagcatcag aatctgttgt tttagaaaat
    12181 gaagattcag ttattgataa agaggaaagc agcaactttt cgaaggagtc aactttgaat
    12241 cgaacctctg aagtccctgt cgcagaagat gattctttct tagaattaga aagagataca
    12301 gcattgtcag aatcacatca accagttacc tctgaaattc atcctttaga gtcagaagat
    12361 acctctgaaa ttcctgtaaa agaagacgat agttttttag aactagaaga tagggctaag
    12421 acaaaggtgg cagatacttc tgaggtagga aatgtagttc ctgattccac gactctttct
    12481 gacaatgttt ctgctaaatc tgaggcatct tttagtgatg aggagcaatc aagtgatagt
    12541 caggcttcag agcagttttc agaaactccc cttcaagaag agatgtctag tggaaaaact
    12601 gaggtacaaa cagagtcaga ggatacatct gcggctgatg cttttttagc agattactat
    12661 gctaaacgta aagcaattga gaaagaaatc tcgtctaatt cgctgtcaac agatgagtca
    12721 gaactttctg aggcacagga agttttatcg caatcacagg cagacactat aaaagctgag
    12781 agtcaagagg aaaaatataa tcgtagcctg aaaaaaacac gcacaggttt tagtgcacgc
    12841 cttaatgctt tcctttctaa cttccgtcgt gttgacgaag agttctttga agaattagaa
    12901 gaaatgttaa tcttatctga cgttggggta aatgttgcaa cacagttaac tgaagacctt
    12961 cgttatgaag caaaattaga gaatgccaag aaatcagagg atttaaaacg tgttatcgtt
    13021 gaaaaattag ttgaaatcta cgaaaaagat ggtatttata atgaggctat caatttccaa
    13081 gaaggtttaa ccgtaatgct ttttgtaggt gttaatggtg ttggaaaaac gacatctata
    13141 ggtaagttag ctcatcaata caagtctcaa gggaaaaaag ttatgttagt agcagctgat
    13201 actttccgtg caggtgctgt ggctcaacta gtagaatggg gacgccgtgt tgatgtacca
    13261 gttgtcactg gagaaaaaaa agcagatccg gctagcgttg tctttgatgg catggaaaaa
    13321 gccgttgctc aaggcgttga tgtcttgtta attgatacag caggacgttt gcaaaataaa
    13381 gaaaacctta tggcagagtt ggaaaaaatt ggacgtatta ttaagcgtgt cgtaccagat
    13441 gctccccacg aaactttgct tgctttagat gcatctacag gacaaaatgc tttaagtcaa
    13501 gctaaagagt tctcgaaaat tacaccatta actggcctta ttctgactaa aattgatggt
    13561 actgctaagg gtggtgtggt actggctatt cgtcaagaac tagatattcc tgttaaattc
    13621 attggtttcg gcgaaaaaat cgatgatatt ggtgaattta attcagaaga tttcatgcgt
    13681 ggccttctgg aaggaatttt ataagaaaaa agaactttca tttagaaagt tctttttata
    13741 gtacgacggg catgtcgtac atctgaggtg caagtcctct gtgggcaccc gctaccggtg
    13801 aacccaatag cgactcccaa gcttgactat cgtgaggtag cggggagagg aagggatagc
    13861 gaaatc
//
LOCUS       DATRQA010000046        13540 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.046, whole genome shotgun sequence.
ACCESSION   DATRQA010000046 DATRQA010000000
VERSION     DATRQA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13540
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.046"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            51..2123
                     /locus_tag="VBD27_000992"
     CDS             51..2123
                     /locus_tag="VBD27_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO7025017.1"
                     /translation="MKKKIILKSSVLGLVAGTSIMFSSVFADQVGVQVIGVNDFHGAL
                     DNTGTANMPDGKVTNAGTAAQLDAYMDDAQKDFKQTNPNGESIRVQAGDMVGASPANS
                     GLLQDEPTVKTFNAMNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNITKSYPH
                     EAAKQEIVVANVIDKVNKQIPYNWKPYTIKNIPVNNKSVNVGFIGIVTKDIPNLVLRK
                     NYEQYEFLDEAETIVKYAKELQAKNVKAIVVLAHVPATSKDDIAEGEAAEMMKKVNQL
                     FPENSVDIVFAGHNHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAK
                     VIAVAPGKKTGSADIQAIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDNVSPVGSL
                     ITEAQLAIARKSWPDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEI
                     TGRDLYKALNEQYDQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPD
                     AKYKLVINDFLFGGGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVSIPNNKP
                     KIYVTMKMVNETITQNDGTYSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVT
                     TIHKKQLHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIAL
                     NTKKKHMK"
     gene            complement(2160..2570)
                     /locus_tag="VBD27_000993"
     CDS             complement(2160..2570)
                     /locus_tag="VBD27_000993"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO7025018.1"
                     /translation="MIKPIVRDTFFLQQKSQMASRADVSLAKDLQDTLHANQNYCVGM
                     AANMIGSLKRVIIINVGITNLVMFNPVLVAKSDPYETEESCLSLVGCRSTQRYHHITI
                     SYRDINWKEQQIKLTDFPAQICQHELDHLEGILI"
     gene            complement(2640..3989)
                     /gene="gdhA"
                     /locus_tag="VBD27_000994"
     CDS             complement(2640..3989)
                     /gene="gdhA"
                     /locus_tag="VBD27_000994"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEO7025019.1"
                     /translation="MTSGKTYVASVLEKVKKQNEHEEEFLQAVEEVFESLVPVFDKYP
                     QYIEENLLERLVEPERVISFRVPWVDDKGQVQVNRGYRVQFSSAIGPYKGGLRFHPTV
                     TQSIVKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDNEVMRFTQSFMTELQKYIG
                     PDLDVPAGDIGVGGREIGYLYGQYKRLNGYQNGVLTGKGLTYGGSLARTEATGYGAVY
                     FAKEMLAARGQDLTGKVALVSGSGNVAIYATEKLQELGATVVAVSDSSGYVYDPDGID
                     LETLKQIKEVERARIVKYTEKHPKANFTPADQGSIWSIKADLAFPCATQNELDEEDAK
                     LLVENGVLAVTEGANMPSTLGAIKVFQKAGVAFGPAKAANAGGVAVSALEMAQNSSRR
                     AWTFEEVDQELQRIMKTIFVNASKAADEFGDSGNLVLGANIAGFLKVAQAMSAQGIV"
     gene            4157..4666
                     /locus_tag="VBD27_000995"
     CDS             4157..4666
                     /locus_tag="VBD27_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO7025020.1"
                     /translation="MKWFPLVGGIIALLAGFYLFINPVTVVATLGWFIALFIFIYGIS
                     NLVRYLQADSYHRSPWYLVQSILAIIFGIILLSSSAMSLSNTVMTILAYWLLVSGILR
                     LLGGFQMQKINFPGAKSYKRSAYIALILGIILLVQPTLSAAIIGRFVAFILIAIGASA
                     IYTFTRINY"
     gene            complement(4707..6476)
                     /locus_tag="VBD27_000996"
     CDS             complement(4707..6476)
                     /locus_tag="VBD27_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025021.1"
                     /translation="MRKKSIFLRLWSYLTRYKATLFLAIFLKVLSSFMSILEPFILGL
                     AITELTANLVDMAKGVSGAELNVPYIAGILIIYFFRGVFYELGSYGSNYFMTTVVQKS
                     IRDIRHDLNRKINKVPVSYFDKHQFGDMLGRFTSDVETVSNALQQSFLQIINAFLSII
                     LVVVMVLYLNVPLAMIIIACIPVTYFSAQAILKRSQPYFKEQAKILGELNGFVQEKLT
                     GFNIIKLYGREEASSQEFRDITDNLRHVGFKASFISGIMMPVLNSISDFIYLIIAFVG
                     GLQVIAGTLTIGNMQAFVQYVWQISQPVQTITQLAGVLQSAKSSLERIFEVLDEEEEA
                     NQVTEKLSHDLTGQVSFHGVDFHYSPDKPLIRDFNLDVEPGQMIAIVGPTGAGKTTLI
                     NLLMRFYDVSEGAITVDGHDIRHLSRQDFRQQFGMVLQDAWLYEGTIKENLRFGNLEA
                     SDEDIVAAAKAANVDHFIRTLPGGYNMVMNQESSNISLGQKQLLTIARALLADPKILI
                     LDEATSSVDTRLELLIQKAMKKLMEGRTSFVIAHRLSTIQEADNILVLKDGQIIEQGN
                     HQKLLADKGFYYELYNSQFSNSK"
     gene            complement(6481..8220)
                     /locus_tag="VBD27_000997"
     CDS             complement(6481..8220)
                     /locus_tag="VBD27_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025022.1"
                     /translation="MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGV
                     TKGDRTGVYLWTFIMFIFVVLGIIGRITMTYASSRLTTTMIRDMRNDMYAKLQEYSHH
                     EYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPSLAWLVA
                     VAMPLLVGVILYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKF
                     QVANQRYTDTSTGLFKLTGLTEPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFI
                     EYSFHALFSFLLFANLFTMYPRMVVSSHRIREVMDMPISINPNTEGVTDTKLKGHLEF
                     DNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGKIL
                     VDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAK
                     EFIESHQEAFETHLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDAT
                     LRARLKEVTGDSTVLIVAQRVGTIMDADQIIVLDEGEIVGRGTHAQLIENNAIYREIA
                     ESQLKNQNLSEGE"
     gene            complement(8287..8760)
                     /locus_tag="VBD27_000998"
                     /pseudo
     CDS             complement(8287..8760)
                     /locus_tag="VBD27_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(8757..10625)
                     /locus_tag="VBD27_000999"
     CDS             complement(8757..10625)
                     /locus_tag="VBD27_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7025023.1"
                     /translation="MSDFLVDGLTKSVGDKTVFSNVSFIIHSLDRIGIIGVNGTGKTT
                     LLDVISGELGFDGDRSPFSSANDYKIAYLKQEPDFDDSQTILDTVLSSDLREMALIKE
                     YELLLNHYEESKQSRLEKVMAEMDSLDAWSIESEVKTVLSKLGITDLQLSVGELSGGL
                     RRRVQLAQVLLNDADLLLLDEPTNHLDIDTIAWLTNFLKNSKKTVLFITHDRYFLDNV
                     ATRIFELDKAQITEYQGNYQDYVRLRAEQDERDAASLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFQNLKNDLHQTSDTSDLEMTFETSRIGKKVINFENVSFSYPDKSILKD
                     FNLLIQNKDRIGIVGDNGVGKSTLLNLIVQDLQPDSGNVSIGETIRVGYFSQQLHNMD
                     GSKRVINYLQEVADEVKTSVGTTSVTELLEQFLFPRSTHGTQIAKLSGGEKKRLYLLK
                     ILIEKPNVLLLDEPTNDLDIATLTVLENFLQGFGGPVITVSHDRYFLDKVANKIIAFE
                     DNDIREFFGNYTDYLDEKAFNEQNNEVISKKESTKTSREKQSRKRMSYFEKQEWATIE
                     DDMMILENTITRIEDDMQTCGSDFTRLSDLQKELDAKNEALLEKYDRYEYLSGLDT"
     gene            complement(10636..11844)
                     /locus_tag="VBD27_001000"
     CDS             complement(10636..11844)
                     /locus_tag="VBD27_001000"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEO7025024.1"
                     /translation="MRLNYLPSEFQKALPILKKIKKAGYEAYFVGGSVRDVLLDRPIH
                     DVDIATSSYPEETKQIFKRTVDVGIEHGTVLVLEKGGEYEITTFRTEEVYVDYRRPSQ
                     VNFVRSLEEDLKRRDFTVNAFALNEDGEVIDLFHGLDDLDNHLLRAVGLASERFNEDA
                     LRIMRGLRFSASLNFDIETTTFEAMKKHAPLLEKISVERSFIEFDKLLLAPYWRKGML
                     ALIDSHAFNYLPCLKNRELQLSAFLSQLDKDFLFETSEQAWASLILSMEVEHTKTFLK
                     KWKTSTHFQKDVEHIVDVYRIREQMGLTKEHLYRYGKTIIKQAEGIRKARGLMVDFEK
                     IEQLDSELAIHDRHEIVVNGSTLIKKLGIKPGPQMGDIISQIELAIVLGQLINEEEAI
                     LHFVKQYLMD"
     gene            complement(12029..12877)
                     /locus_tag="VBD27_001001"
     CDS             complement(12029..12877)
                     /locus_tag="VBD27_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7025025.1"
                     /translation="MKLALITDTSAYLPEAIENHEDVYVLDIPIIIDGKTYIEGQNLT
                     LDQYYDKLAASKELPKTSQPSLAELDDLLCQLEKEGYTHVLGLFIAAGISGFWQNIQF
                     LIEEHPNLTIAFPDTKITSAPQGNLVRNALMCSREGMDFDVIVNKIQSQIEKIEGFIV
                     VNDLNHLVKGGRLSNGSAIIGNLLSIKPVLHFNEEGKIVVYEKVRTEKKALKRLAEIV
                     KEMTADGEYDIAIIHSRAQDKAEQLYNLLAKAGLKDDLEIVSFGGVIATHLGEGAVAF
                     GITPKN"
     gene            complement(12877..13257)
                     /locus_tag="VBD27_001002"
     CDS             complement(12877..13257)
                     /locus_tag="VBD27_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEO7025026.1"
                     /translation="MEVIREQEFVNQYHYDARNLEWEEENGTPKTNFEVTFQLANRDE
                     AAKVTSIVAVLQFVIVRDEFVISGVISQIAHIQGRLINEPSEFSQDEVENLAAPLLEI
                     VKRLTYEVTEIALDRPGVTLEFNS"
     gene            complement(13329..>13540)
                     /locus_tag="VBD27_001003"
     CDS             complement(13329..>13540)
                     /locus_tag="VBD27_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025027.1"
                     /translation="LLKLFLKEKNVKGLSKMKRSDLDQAIAIAFTEEELGKLFVVRGL
                     ALTEKGLAALSNNQEVIDRHPKKKF"
BASE COUNT         4363 a         2735 c         2092 g         4350 t
ORIGIN      
        1 atgatagtaa aatacaaatg taatacttat ttaatttaca ggagatttaa atgaaaaaga
       61 aaattatttt gaaaagtagt gtccttggtt tagtcgctgg gacttctatt atgttctcaa
      121 gcgtattcgc tgaccaagtc ggcgtccaag ttataggcgt caatgacttt catggtgcac
      181 ttgacaatac tggaacagca aatatgcctg acggaaaagt tactaatgct ggcactgctg
      241 ctcaattaga tgcttatatg gatgatgctc aaaaagattt caaacaaact aaccctaatg
      301 gtgaaagcat tagagttcaa gctggtgata tggttggagc aagtccagct aactcagggc
      361 ttcttcaaga tgaaccaacc gttaaaacat ttaatgcaat gaatgttgag tatggcacat
      421 taggtaacca tgaatttgat gaaggtttgg cagaatacaa tcgtatcgtt actggaaagg
      481 cccctgctcc agattctaat ataaataata ttacgaaatc atacccacac gaagctgcaa
      541 aacaagaaat tgtagtggca aacgttattg ataaagttaa caaacaaatc ccttacaatt
      601 ggaaacctta cactattaaa aatattcctg taaataacaa aagtgtgaac gttggcttta
      661 tcggaatcgt taccaaagac atcccaaacc ttgtcttacg taaaaattat gaacaatatg
      721 aatttttaga tgaagctgaa acaatcgtta aatacgccaa agaattacaa gctaaaaatg
      781 tcaaggctat tgtagtcctt gctcatgtac ctgcaacaag caaggatgat attgctgaag
      841 gtgaagcagc agaaatgatg aaaaaagtca atcaactctt ccctgaaaat agcgtagata
      901 ttgtctttgc tggacacaat catcaatata caaatggtct tgttggtaaa actcgtattg
      961 tacaagcgct ctctcaagga aaagcctatg ctgatgtacg tggtgtccta gatactgata
     1021 cacaagattt cattgaaacc ccttcagcta aagtaattgc agttgctcct ggtaaaaaaa
     1081 caggtagtgc cgatattcaa gccattgttg accaagctaa tactatcgtt aaacaagtaa
     1141 cagaagctaa aattggtact gccgaggtaa gtggcatgat tacgcgttct gttgatcaag
     1201 ataatgttag tccggtaggc agcctcatca cagaggctca actagcaatt gctcgaaaaa
     1261 gctggccaga tatcgatttt gccatgacaa ataatggtgg cattcgtgct gacttactca
     1321 tcaaaccaga tggaacaatc acctggggag ctgcacaagc agttcaacct tttggtaata
     1381 tcttacaagt cgtcgaaatt actggtagag atctttataa agcactcaac gaacaatacg
     1441 accaaaaaca aaatttcttc cttcaaatag ctggtctgcg atacacttac acagataata
     1501 aagagggcgg ggaagaaaca ccatttaaag ttgtaaaagc ttataaatca aatggtgagg
     1561 aaatcaatcc tgatgcaaaa tacaaattag ttatcaatga ctttttattc ggtggtggtg
     1621 atggctttgc aagcttcaga aatgccaaac ttctaggagc cattaatccc gatacagagg
     1681 tatttatggc ctatatcact gatttagaaa aagctggtaa aaaagtgagc attccaaata
     1741 ataaacctaa aatctatgtc actatgaaga tggttaatga aactattaca caaaatgatg
     1801 gtacatatag cattattaag aaactttatt tagatcgaca aggaaatatt gtagcacaag
     1861 agattgtatc agacacttta aaccaaacaa aatcaaaatc tacaaaaatc aaccctgtaa
     1921 ctacaattca caaaaaacaa ttacaccaat ttacagctat taaccctatg agaaattatg
     1981 gcaaaccatc aaactccact actgtaaaat caaaacaatt accaaaaaca aactctgaat
     2041 atggacaatc attccttatg tctgtctttg gtgttggact tataggaatt gctttaaata
     2101 caaagaaaaa acatatgaaa taataaaaat aggtttggat aaaatatcca aacctatttt
     2161 tatatcaata tgccttccaa atgatctaac tcgtgttgac aaatttgagc tggaaaatct
     2221 gtcaacttga tttgctgttc tttccaattg atatcacgat aagaaatcgt aatgtgatga
     2281 tatcgttgtg tacttctgca acctacgaga gaaaggcaag attcttctgt ctcatatgga
     2341 tccgattttg caactaatac agggttaaac attactaaat ttgtaatacc cacattgatg
     2401 ataatgacac gcttcagact accaatcata ttggctgcca tacctacaca ataattttga
     2461 ttagcatgca aagtatcctg taaatctttt gctaagctca catctgctct actagccatt
     2521 tgtgattttt gttgtaaaaa gaaagtatct ctaacaattg gttttatcat ttattttaac
     2581 ctaactctat tatgataata ctagtatacc ataggttagc taaggactat gataagagat
     2641 taaacaatac cttgtgcaga catagcttga gcaactttta aaaagcctgc aatgttagca
     2701 cctagaacca ggttcccact atcgccaaat tcatcagccg ctttggaagc attcacaaaa
     2761 atagttttca taatacgttg caattcttga tcaacttctt caaaagtcca tgccctacga
     2821 ctagaatttt gtgccatttc aagcgctgaa acagctactc ctcctgcatt ggcagcttta
     2881 gcaggaccaa aagcaacccc tgccttttgg aaaactttaa tagcacccaa ggtagatggc
     2941 atatttgctc cttctgttac tgccaagaca ccattttcaa ctaataactt agcgtcttct
     3001 tcgtctaatt cgttttgagt agcacacggg aaagctaaat ctgctttaat agaccagata
     3061 ctaccttgat ctgctggtgt gaaattagct tttggatgtt tctccgtata tttaacaata
     3121 cgtgctcttt caacctcttt tatctgtttc aatgtttcaa ggtcaatacc gtctggatca
     3181 taaacataac ctgaagaatc tgacacagct acaacagttg cacccaactc ttgtaatttt
     3241 tcagtagcat aaatagccac gttaccagat cccgaaacca gtgcaacttt gccagttaaa
     3301 tcttgaccac gcgctgccag catttcttta gcaaaataga ctgccccata accagttgct
     3361 tctgtacgag ccaaagagcc accataagtc agtccttttc cggttaaaac cccattttga
     3421 taaccattta aacgcttata ttgaccataa agataaccaa tttcacgtcc acctactcca
     3481 atatcgccag ctggcacatc taaatctggt ccaatgtatt tttgaagttc ggtcataaaa
     3541 ctttgtgtga aacgcatcac ttcattatct gatttccctt ttggatcaaa gttcgaccct
     3601 cctttgccac caccaattgg tagacctgtc agtgaattct taaaaatttg ttcaaatcct
     3661 aaaaatttga caatagattg tgttactgta gggtgaaaac gtaaaccacc tttataagga
     3721 ccaatagctg atgaaaattg tacacgatat cctcggttaa cttgaacttg gcctttatca
     3781 tctacccatg gaactctaaa agaaatcacg cgctcaggct caaccaaacg ttctaaaaga
     3841 ttttcttcaa tgtactgagg gtatttatca aaaactggta ctagtgactc aaaaacctct
     3901 tcaaccgctt gtagaaattc ttcctcatgc tcattttgct tcttcacttt ctctaaaaca
     3961 cttgctacat atgtctttcc gcttgtcatt tattttcctc cgtattgttt taatatagtt
     4021 agcattataa attgtcagaa ttttcttgtc aactatttgc tatggtatca ttattaggag
     4081 caattttaaa ggtaattatt tgacttttac tgactttcgt tctataatga gacaaatatt
     4141 tgattggagt atctatatga aatggttccc acttgttgga ggtataattg ccttattagc
     4201 tggcttctat ctctttataa atcctgtaac ggttgttgca actctgggtt ggttcattgc
     4261 tttatttatc tttatttatg gcatttcaaa tttagtacgc tacttgcaag ctgattctta
     4321 tcaccgtagc ccttggtatc tggttcagag tatccttgct attatttttg gtatcatttt
     4381 actatccagc tcagcaatgt cactttcaaa tactgtcatg actattttag catactggct
     4441 actggtatct ggtattttac gacttttggg tggtttccaa atgcaaaaaa ttaatttccc
     4501 aggtgccaaa tcctataaac gttcggctta tattgcactc attttaggta ttattttact
     4561 ggttcaacct accttatcag cagccatcat tggaagattt gtagccttta tattaatagc
     4621 aattggagct tcggctattt acacttttac tcgtattaat tattaaaagg acatgtttcg
     4681 tgtccttttt ttatctattg atgacgtcat ttactatttg aaaattggct attatataat
     4741 tcgtaataaa atcctttatc tgctaggagt ttctggtgat taccttgttc gatgatttga
     4801 ccatccttta gaactaaaat gttgtctgcc tcttgaatcg ttgatagacg gtgtgcaatg
     4861 acaaaactag ttcttccttc catcaatttt ttcatagctt tttgaataag caattccaac
     4921 cttgtatcaa cagatgaagt cgcttcatct aaaattaaaa ttttaggatc tgctaataaa
     4981 gcacgtgcaa tagtcaacag ttgcttttgt cctaaggaga tattgcttga ttcttgattc
     5041 atcaccatat tgtagccacc aggtaacgtt cgaataaagt ggtcaacatt cgcagctttg
     5101 gcagcagcaa ctatatcctc atcactggct tcaagattac cgaatctaag attttctttt
     5161 attgtacctt catataacca agcatcttgc aacaccatgc caaattgttg ccggaaatct
     5221 tggcgtgata ggtgtctaat atcgtgacca tctacggtga tagcaccttc actaacatcg
     5281 tagaatctca tgagtaagtt tataagtgtt gtcttacctg cacctgttgg ccctacaatt
     5341 gcaatcattt gccctggttc tacatctaag ttaaagtcgc ggataagtgg cttatccgga
     5401 gaatagtgaa aatcgacacc atgaaagctg acttgaccgg ttaagtcatg gctcaatttt
     5461 tcagtgactt gattggcttc ctcttcttca tctaagactt caaaaatacg ttctaaggaa
     5521 gattttgcac tttgcaaaac acctgctaat tgtgtaatgg tttgaacggg ttgactaatt
     5581 tgccaaacat actgcacaaa tgcctgcatg ttaccaattg ttaaggtacc agcaataact
     5641 tgtaaaccgc caacaaatgc aatgataaga taaataaaat cagagatact attcaatact
     5701 ggcatcataa ttcctgaaat gaagctagct ttgaaaccta catgacgtag attatccgtt
     5761 atatcacgaa attcttgact agacgcctct tctctaccat ataattttat gatattgaaa
     5821 ccagttaatt tctcttgtac aaaaccattc agttctccca gaatctttgc ctgttcttta
     5881 aagtaaggtt gggatctttt tagtatcgct tgtgcactaa aataagttac aggaatacaa
     5941 gcaatgataa tcatggctaa tggtacattt agataaagta ccataacgac tactagaata
     6001 atacttaaaa aagcattgat aatctgtaaa aagctctgtt gtaaagcatt tgagaccgtt
     6061 tcaacatcac ttgtaaaacg tcccaacata tctccaaatt ggtgcttatc aaaatacgat
     6121 acaggaactt tattgatttt acgattcaaa tcatgacgga tatcacggat acttttttga
     6181 acgacagtag tcataaaata atttgagcca taagaaccta attcatagaa aacacctctg
     6241 aaaaaataaa taatcagaat accagcaata taaggaacgt tcaattctgc cccagaaact
     6301 cccttagcca tatcaacaag gttagcagtc aactctgtta tcgctaaccc taaaataaaa
     6361 ggctccagaa tactcataaa actagataaa actttcaaaa aaatcgctaa gaaaagagta
     6421 gctttgtagc gagttaggta agaccataat ctcaaaaaaa tagatttttt tctcatacaa
     6481 tcactctcct tctgataagt tttggttctt cagttgtgac tcagcgattt cacgataaat
     6541 agcattattt tctattaatt gagcgtgggt accacgaccg acaatttcgc cttcatcaag
     6601 gacaataatc tgatcagcat ccataatcgt acccaccctt tgagcaacta tcaaaaccgt
     6661 agaatcaccg gttacttctt ttagacgcgc tcttaaagta gcgtctgtct tataatcgag
     6721 agcagaaaat gaatcatcaa aaatatataa atctggatct ttaacaacag ccctagcaat
     6781 ggataaccgt tgtttttgac ccccagaaag attgctccca ccttcagcta aatgcgtttc
     6841 aaaggcttct tggtgactct caataaactc tttagcttga gaaatatcaa ccgcttgtct
     6901 aagatcatca atagtagcat cagcttttcc atattttaaa ttctctccta ttgtccctgt
     6961 aaataaaaga gctttttggg ggataaatcc aatcttttgg cgaagtgatt taaggttata
     7021 atctcttaca tcaactccat ctactaagat ttttccaagt gtcacatcat aaaaacgtgg
     7081 aatcaaatta acaagagaag attttcctga acctgttgaa ccaataaaag caattgtttc
     7141 tccaggctta gctttaaaag aaatatcatg caaaacggga ctctctgttt ctcctggata
     7201 agcgaatgtt acattatcaa attctaaatg ccctttaagt ttcgtatccg taacaccttc
     7261 ggtattagga ttaatagaaa tcggcatatc cattacctct ctaatacgat ggctagatac
     7321 caccatacga ggatacatag taaaaagatt ggcaaatagc aaaaatgaaa agagagcatg
     7381 gaagctatat tcgataaaag caactaaatc ccctatttta atagcacctc tttgtaaggg
     7441 atccaaagca aaccaaacga tagccacaat cattgcaata ataatttgaa cgaaaagtgg
     7501 ttctgttagc cctgttaatt taaaaagacc agttgaagta tctgtgtaac gttggttagc
     7561 gacttgaaat ttttgtgatt gaaaattctc tcttgcaaag gccctaacaa cgcgtaaccc
     7621 tgttaaattt tcacgaacat attgattgat tttatcaagc atagtctgtt gtctttcaga
     7681 taaaggtttt gtttttatag ctacatataa aatgactcct accaaaagag gcatcgcaac
     7741 cgctacaagc caagccaaag atggactcgt aattagtatc ataaccacgc taaaaatcat
     7801 taccatagga gttactaggc ctaaacgtaa agacatttca gcaaattgca tcaaaacaaa
     7861 agtatcgctt gtcatacgtg tcactagtga agatacacct atctgttcat attcatgatg
     7921 ggagtattct tgaagcttag catacatatc attacgcata tctctaatca ttgttgtcgt
     7981 taagcgacta gatgcgtaag tcatcgtaat acgcccaata atacctagta caacaaatat
     8041 aaacatgatg aacgtccaca gataaactcc agtccgatca ccttttgtaa cgccattatc
     8101 aatcataccc gctaaggctg tgggcattcc taggataacc gtcacaaaaa gcatagctcc
     8161 tagtaaatca agccataacc aatttgggta ccttttcaaa taagaccaaa tatatgccat
     8221 atatgtctcc ttttctttcc tcttatctca aaataattaa tccatgttca ctaatccact
     8281 ttcaacttat aaatctttaa tcatccaaat atccatagct gtatgacctt gatcatttgc
     8341 tattggttgc gataaggcac aatatcccat atgcttataa accgcagttg ccctactcaa
     8401 aatactggct gtctctaaat aaagttgtct atacccaatt tttcgagctt ctacttcaat
     8461 cattttcact aaatcagtag caagcccctt accacggaaa cgttctgcaa tgtacacctt
     8521 ctgcatctct gcaattagat ttttcagcgg accaaagccg cttcagccaa taatctcatc
     8581 tctctcctca atgacaaaga atcctgactt ctctattttt tcgtagtatg aggtcaagtg
     8641 atctaagtga gggtctgaat atgctgtacc aggtttatct aaatcatagg cgcgtaaact
     8701 ctgtcgaatt aattgtgtaa ctgcttggtc atcattttta ataatcggac ggataatcat
     8761 gtgtctaacc cactaaggta ctcataacgg tcatactttt ctagaagtgc ttcatttttt
     8821 gcatctaatt ccttttgtaa atcagataac cttgtaaaat cactaccaca tgtttgcata
     8881 tcatcttcta tacgagtgat agtattttcc aatatcatca tatcgtcttc aattgtcgcc
     8941 cattcttgtt tttcaaagta agacattctt ttacgacttt gcttttcacg acttgtcttg
     9001 gtactctctt ttttactgat aacttcatta ttttgctcat taaatgcttt ttcatctaaa
     9061 taatcagtat aattaccaaa aaattcacgg atatcgttat cttcaaatgc aataatttta
     9121 ttagccactt tatctaaaaa gtaacgatcg tgactaactg taatcacagg accaccaaag
     9181 ccttgtaaaa aattttcaag aacagttaat gtagcaatat ctaagtcatt tgtcggctca
     9241 tcaagtagta acacgttagg cttttcaatc aggattttta aaaggtaaag tcgttttttc
     9301 tcaccacctg ataattttgc aatttgtgtt ccatgtgtcg aacgtggaaa gagaaattgt
     9361 tccaatagtt ctgtcacact tgttgtaccg acactagttt taacctcatc tgcaacctct
     9421 tgcaaataat taataacacg ttttgagcca tccatattat gaagttgttg tgaaaagtaa
     9481 cctacacgta tcgtttcacc aatagagaca ttacccgaat ccggctgtaa atcttgaaca
     9541 attaaattaa gtaaggttga ctttccaaca ccattatctc caacgatgcc aatacggtct
     9601 ttattttgaa ttaacaaatt aaagtctttc aagatagatt tatctgggta agaaaaagag
     9661 acattttcaa aattaataac ctttttccca attcgacttg tttcaaatgt catttccaaa
     9721 tcgcttgtat cgcttgtttg gtgtaaatcg ttttttagat tttgaaaacg attaatacga
     9781 gcctgttgtt tcgttgcacg agcttgtggc tgagtacgca tccaagctag ttcctgttta
     9841 taaagctgtt tctttttatg taaactagca gcatcacgct cgtcttgttc tgcacgaagt
     9901 cggacataat cctgataatt gccttgatat tctgtaatct gtgccttatc taattcaaaa
     9961 atacgtgttg caacattgtc tagaaaataa cgatcatgag ttataaaaag cactgtcttt
    10021 ttactatttt tcaaaaaatt cgttaaccat gcaatagtgt caatatctaa gtggttagta
    10081 ggttcgtcta agagcaataa atctgcatca tttaataata cttgcgctaa ttgaacacgt
    10141 cttcgtaatc ctcctgataa ttcaccaacc gacaactgca aatcagtaat acctaatttg
    10201 gataatactg ttttgacttc gctctcaata gaccaagcat ctaaagaatc catttctgcc
    10261 attactttct ctagacgtga ttgcttactt tcttcgtagt gattaagcaa taattcatat
    10321 tctttaatta aagccatctc tcttaagtca gaagaaagta cggtgtccaa aattgtctga
    10381 gaatcatcaa agtctggttc ttgttttaaa taagcaatct tataatcatt agctgatgaa
    10441 aaaggggaac gatcaccatc aaaacctaat tcacccgaaa taacatctaa tagtgttgtc
    10501 tttccagttc cattgacacc aataatccca atacggtcta aactatggat gataaatgaa
    10561 acattactaa agaccgtctt atcaccaacc gacttagtca atccatctac taaaaaatcg
    10621 ctcatataat cctctctaat ccatcaagta ctgcttaaca aaatgtaaaa tagcctcttc
    10681 ttcattaatc agttgtccta aaacaatggc taattcaatt tgagagataa tatctcccat
    10741 ctgtggacca ggttttattc ctaatttctt gattaaggtg ctaccattga caactatctc
    10801 atgcctatca tggatagcta actcactatc tagttgttct attttttcga aatcaaccat
    10861 caagcctctt gctttgcgaa taccttccgc ttgttttatt atagtttttc cataacgata
    10921 aagatgttct ttagtcaatc ccatttgttc acgaatacga taaacatcca ctatatgctc
    10981 aacatctttt tgaaagtgag ttgatgtctt ccattttttt aaaaaagtct ttgtgtgttc
    11041 aacttccata ctcaagatga gtgatgccca agcttgttct gatgtctcaa ataaaaaatc
    11101 tttatctaac tgggacaaaa aggcgcttaa ttgtaattcc ctatttttta agcatggtag
    11161 ataattaaaa gcatgactat ctattagtgc cagcattcct ttacgccaat aaggtgctaa
    11221 tagaagttta tcaaattcaa taaaagaacg ctcaacagat attttctcca ataatggcgc
    11281 gtgctttttc attgcctcaa aagttgttgt ttcgatatca aaatttagac tagcagaaaa
    11341 acgcaaacct cgcataattc gaagagcatc ttcattaaaa cgctcactgg cgagtcctac
    11401 tgctctaaga aggtgattat ctaaatcatc caatccatga aagagatcaa taacttctcc
    11461 atcttcattt aaagcaaagg cattaactgt gaaatctcga cgttttagat cttcttctag
    11521 cgacctaaca aaattgacct gactcggacg cctataatca acataaacct cctcagtcct
    11581 aaaagtggta atctcatatt caccaccttt ttccaatact aagactgttc catgttcaat
    11641 accaacatca actgtccttt taaaaatctg ctttgtttct tctggataag atgacgttgc
    11701 tatatcaaca tcatgaattg gacggtcaag taaaacatcc cgaacactac cacctacaaa
    11761 gtatgcttca taaccggctt tttttatctt ttttaatatt ggtaaagcct tctgaaactc
    11821 agaaggcaaa taatttaatc tcatcatact attccaaatc taaacgaact aaagtcgcat
    11881 tattatttct tataaatgga ttcagaaaca aaaagaactg cctaagggac actccctacc
    11941 tactcaaatt atcaaaaaag aatctcctaa cttcttcttt gatatctgca ataaggatca
    12001 acctccaagc aactcttttt atcacacatc aattttttgg ggtaatacca aaggcaaccg
    12061 caccttcacc aagatgagtt gcaataacgc caccaaatga tacaatttct aaatcgtctt
    12121 taagaccagc tttagctaac aaattatata attgttctgc cttatcttgt gctctagagt
    12181 gaataatagc aatatcatat tcaccatctg ctgtcatttc tttgacaatc tcagccaagc
    12241 gttttaaggc ttttttctct gtacgcactt tctcatagac aacgatttta ccttcttcat
    12301 taaaatgtaa gaccggcttg atacttaaaa ggttaccaat gatagctgaa ccgtttgaca
    12361 atctaccacc ttttactaga tgattcaaat cattaacaac aataaatcct tcaatttttt
    12421 caatttgtga ttgtatcttg ttaactatga catcaaaatc catcccttca cgtgaacaca
    12481 tcaaagcatt tctaactaaa ttgccctgtg gtgctgaagt tattttagta tccggaaaag
    12541 caattgtcaa atttggatgt tcttcaataa gaaactgaat attctgccag aaacctgata
    12601 tccccgctgc aatgaataaa ccaaggacgt gtgtataccc ttctttctcc aattgacata
    12661 agagatcatc tagttcagct aagctaggct gacttgtttt aggtaattct tttgatgccg
    12721 ctagcttatc ataatattgg tctaatgtca gattttgtcc ctcaatataa gttttaccat
    12781 caatgataat tggaatatct aaaacataga catcttcatg attttcgata gcctcaggca
    12841 agtaagctga ggtatccgta ataagtgcta atttcattaa ctattaaact ccaatgttac
    12901 tcctggtcta tctaaagcaa tttctgtaac ttcataagtc agacgtttaa cgatttctaa
    12961 aagaggagct gctagattct caacttcatc ttgactaaac tcactaggct cattgataag
    13021 gcgcccttgg atatgagcta tttgtgaaat gacaccagaa ataacaaact catcacgtac
    13081 aataacaaat tgtaacactg ctacaatgct cgttactttt gctgcttcat ctctattagc
    13141 taattggaaa gtaacttcaa aatttgtttt tggggtacca ttttcctctt cccattctaa
    13201 attgcgcgca tcatagtggt attgattgac aaattcttgt tcacgaatga cttccatgta
    13261 ttttctcctt gatagacaat atgtattatt atagcataaa tttgtagatt cttaaagcag
    13321 gtgggaatct agaacttctt cttaggatgt ctatcaatca cttcttgatt gttactcaag
    13381 gctgctaaac ctttttctgt aagtgccaac cctctgacaa caaacaactt ccctaattcc
    13441 tcttctgtaa aagcaatagc tattgcctga tctaaatcag agcgtttcat ctttgacaag
    13501 ccttttacgt ttttttcttt taagaatagt tttaaaagtg
//
LOCUS       DATRQA010000047        13358 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.047, whole genome shotgun sequence.
ACCESSION   DATRQA010000047 DATRQA010000000
VERSION     DATRQA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13358
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.047"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..298)
                     /locus_tag="VBD27_001004"
     CDS             complement(<1..298)
                     /locus_tag="VBD27_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025028.1"
                     /translation="MLKTRTHINELETKIALDLVHRYLGIQQLVFSKWRHSLETGSWS
                     SIITINGANIINYIVDDISDSDSISGIICHPKSFHLRPREKKEPIQSIDELDIIW"
     gene            complement(424..3606)
                     /gene="carB"
                     /locus_tag="VBD27_001005"
     CDS             complement(424..3606)
                     /gene="carB"
                     /locus_tag="VBD27_001005"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO7025029.1"
                     /translation="MPKRTDIRKIMVIGSGPIVIGQAAEFDYSGTQACLSLKEEGYQV
                     VLVNSNPATIMTDKDIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMAL
                     SKNGILEELNVELLGTKLSAIDKAEDRDLFKQLMEELNQPIPESEIVNSVEEAIQFAE
                     QIGYPLIVRPAFTLGGTGGGMCDNQEQLVDITTKGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPAQTLSDVENQLLRDASLDIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPITKTTYAMFEPALDYVVAKMPRFPFDKFESGDRKLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVDHIKIADLDNVSDDVLLEKIRKAEDDRLFYLAEALRRHYSIEKLAS
                     LTSIDSFFLDKLRVIVELEDLLSKNRLDINILKKVKNKGFSDKAIASLWQINEDQVRN
                     MRKEAGILPVYKMVDTCAAEFDSATPYFYSTYAVENESLISDKASILVLGSGPIRIGQ
                     GVEFDYSTVHSVKAIRESGFEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIDL
                     EKPEGVILQFGGQTAINLAKDLNKAGVKILGTQLEDLDRAENRKQFEATLQALNIPQP
                     PGFTATTEEEAVNAAQKIGYPVLVRPSYVLGGRAMKIVENEEDLRHYMTTAVKASPDH
                     PVLIDAYLIGKECEVDAISDGQNILIPGIMEHIERAGVHSGDSMAVYPPQTLSETIIE
                     TIVDYTKRLAIGLNCIGMMNIQFVIKDQKVYVIEVNPRAGRTLPFLSKVTHIPMAQVA
                     TKVILGDKLCNFTYGYDLYPASDMVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     INLQKALYKAFEAAYLHMPDYGNIVFTVDDTDKEEALELAKVYQSIGYRIYATQGTAI
                     YFDANGLETVLVGKLGENDRNHIPDLIKNGKIQAVINTVGQNNIDNHDALIIRRSAIE
                     QGVPLFTSLDTAHAMFKVLESKAFTLKVLD"
     gene            complement(3637..4713)
                     /gene="carA"
                     /locus_tag="VBD27_001006"
     CDS             complement(3637..4713)
                     /gene="carA"
                     /locus_tag="VBD27_001006"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO7025030.1"
                     /translation="MKRLLLLEDGSVFEGEAFGADVGTSGEIVFSTGMTGYQESITDQ
                     SYNGQIITFTYPLIGNYGINRDDYESIRPTCKGVIVYEWAEYPSNWRQQMTFDEFLKL
                     KGIPGISGIDTRALTKIIRKHGTMKACLINEGNSIHEALENLQKSVLLNNQIEQVSTK
                     LAYASPGVGKNIVLVDFGLKHSILRELSQRQCHITVVPHTTTAQEILNLNPDGVLLSN
                     GPGNPEQLPNALQMIQEIQGKIPIFGICMGHQLFAKANGAKTYKMTFGHRGFNHAVRH
                     LQTGQVDFTSQNHGYAVSREDFPEALFITHEEINDKTVEGVRHKYYPAFSVQFHPDAA
                     PGPHDTSYLFDEFINMIDDFQQKS"
     gene            complement(4727..5650)
                     /locus_tag="VBD27_001007"
     CDS             complement(4727..5650)
                     /locus_tag="VBD27_001007"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO7025031.1"
                     /translation="MNHQTQTLSLEHFVSLEELSNQEVMSLIKRSIEVKENPSNIGFD
                     KDYYVSNLFFENSTRTHKSFEMAELKLGLKTIEFNADTSSVNKGETLYDTILTMSALG
                     LDVCVIRHPDIDYYKELIASPNIHSAIVNGGDGSGQHPSQSLLDLVTIYEEFGYFKGL
                     KIAIVGDLTHSRVAKSNMQVLKRLGAEIFFSGPKEWYSSQFDEYGQCLPIDQLVDQID
                     VLMLLRVQHERHDGKGVFSKESYHQQFGLTKERYKHLRDTAIIMHPAPVNRDVEIASD
                     LVEADKARIVKQMSNGVYARIAILEAVLNSR"
     gene            complement(5815..7107)
                     /locus_tag="VBD27_001008"
     CDS             complement(5815..7107)
                     /locus_tag="VBD27_001008"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO7025032.1"
                     /translation="MYIIKNGLIIDPQSGFNQVSDMLIDQGKIKQISKEIDIKGIPII
                     DASNKIVAPGLVDIHVHFREPGQTHKENIHTGALSAAAGGFTTVLMMANTNPTISSSE
                     IVKQVKESAAKEAIKIETVATITKSLNGKDLVNFEELLEAGVAGFSDDGIPLTDTKVL
                     QEAMNLARKHDVVLSLHEEDPSLNGVLGINEHIAQKIYHVCGASGLAEYSMIARDAMI
                     AYQTQAKVHIQHLSSSESVEVVDFAQKLGANLTAEVTPQHFSKTEDLLLTKGANAKLN
                     PPLRLEKDRQALIDGLKSGVISIIASDHAPHHIMEKAADNISQAPSGMTGLETSLALG
                     ITYLVSTKELSMIDFLAKMTCNPAQLYGFDAGYLREGGPADIVIFDQAEERIIKAEFA
                     SKSSNSPFIGDKLKGVVHYTICNGEIVYQKDSHAATLV"
     gene            complement(7119..7748)
                     /locus_tag="VBD27_001009"
     CDS             complement(7119..7748)
                     /locus_tag="VBD27_001009"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEO7025033.1"
                     /translation="MDLARQIAMELLDIQAVYLRPQQPFTWASGVKSPIYTDNRVTLS
                     YPETRTLIENGFVKQIQKHFPNVDIIAGTATAGIPHGAIIADKMNLPFAYIRSKAKDH
                     GVGNQIEGRVYSGQKMVIIEDLISTGGSVLEAVTAAQSQGIEVLGVVAIFTYQLAKAE
                     QAFREADIPLVTLTDYNQLIKVAKVNGYITADQLVLLKKFKEDQMNWQS"
     gene            complement(7761..8462)
                     /gene="pyrF"
                     /locus_tag="VBD27_001010"
     CDS             complement(7761..8462)
                     /gene="pyrF"
                     /locus_tag="VBD27_001010"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEO7025034.1"
                     /translation="MLEKCPIIALDFSDLASVTTFLEHFPKEELLFVKIGMELYYSEG
                     PSIIRYIKSLGHRIFLDLKLHDIPNTVRLSMSVLAKLGIDMTNVHAAGGVEMMKAARE
                     GLGEGPILLAVTQLTSTSQEQMQVDQHINLSVVDSVCHYAQKAQEAGLDGVVASAQEV
                     KQIKKQTNEHFICLTPGIRPPQTNQLDDQKRTMTPEQARIVGADYIVVGRPITKAENP
                     YQAYLDIKEEWNRIK"
     gene            complement(8675..9907)
                     /locus_tag="VBD27_001011"
     CDS             complement(8675..9907)
                     /locus_tag="VBD27_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO7025035.1"
                     /translation="MQNDMSVVLRAGKLLIESGAEVYRVEDTMKHFAKALQIENFEAY
                     VVSSSIIASGINRYGKQEAKVCNTDGVTANLGRLEAVNNLSRQIAKQDLVSPEEIVKQ
                     LDLIEHQKDYSLLVTLISYFCGAGSFSLALGSSLLDSFSAAVTGLILGYFLNLMESRI
                     HTGFLLTILGSSVVALSANLLYFSGLGEHRSIIILGALMVMVPGAAFVNSVREFSQNN
                     FSTGLALIMSALLICISISAGVAITIEIIPFAEQMTGSFSGVPNTILEIIIRTLMAGL
                     GTIAFSILYHVPKRYFLDLGILGAISWMLYLILWQQFHMDAIAVFFPGLFITYFSRLL
                     AAKRKCPATVFLATSMFPLIPGLSFYRAVYFLLTGADALAMEYFRSCFVTAFTIAIAI
                     SIVQQIPLSFFIRRKMIK"
     gene            complement(9947..11488)
                     /locus_tag="VBD27_001012"
     CDS             complement(9947..11488)
                     /locus_tag="VBD27_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7025036.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLIGANGEGKST
                     FMSIVTGHLQPDEGKIEWSKYVTAGYLDQHTVLEEGQTVRDVLRTAFDELFKTEARIN
                     DIYISMADDGADVDSLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDSDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQDLVRYSGDYTNFESVYAMKKAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPSFEFKESRTPGRFIFQAKDLQIGY
                     DRALTKPLNLTFERNQKIAIVGANGIGKTTLLKSLLGIIPPISGNVERGDFIDLGYFE
                     QEVPGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQSKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKAYKGSILMVCHEPDFYEGWMDDVWD
                     FNQLS"
     gene            complement(11619..11957)
                     /locus_tag="VBD27_001013"
     CDS             complement(11619..11957)
                     /locus_tag="VBD27_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025037.1"
                     /translation="MQIIKRDGQIVEFDPEKIYQAILKAAQTVYVLDESLRQDLAQVT
                     KKVILDLEDAQVERPTINMIQSLVENRLLDAGYIHIAEHYISYRLQRDLERNGYDDQI
                     TVHLHFEKIK"
     gene            complement(12127..13203)
                     /locus_tag="VBD27_001014"
     CDS             complement(12127..13203)
                     /locus_tag="VBD27_001014"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO7025038.1"
                     /translation="MGYTVAIVGATGAVGTQMIRQLEQSNLPIEQVKLLSSSRSAGKI
                     LHFKDEAIRVEETTKESFYDVDIALFSAGGSISAKFAPYAVKSGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHAMIGHNGIIACPNCSTIQMMIALEPIRQKWGIERVIVSTYQAVSGS
                     GARAVEETKEQLRQVLNDNLSPDQLIATVLPCSSDQKHYPIAFNALPQIDIFTDNDYT
                     YEEMKMTLETKKIMEDATIKVSATCVRIPVLSGHSESIYIETKELASISEIKKAIANF
                     PGAVLQDLPSQQIYPQAINAVGHRETFVGRIRKDLDQENGVHMWVVSDNLLKGAAWNS
                     VQIAETLHKNGLVKPAKELKFELI"
BASE COUNT         4149 a         2658 c         2144 g         4407 t
ORIGIN      
        1 accagatgat atctaactca tcaatagact gaataggttc ctttttttct ctaggtctga
       61 gatgaaaact tttaggatga caaataatac ctgaaatact atcactgtcg ctaatatcat
      121 caacaatgta attgataata ttagcgccat ttattgttat aatagaagac catgaaccag
      181 tttctaaaga atgtctccac tttgaaaaaa caagttgttg aatacctaga tatctgtgta
      241 ctaaatcaag ggcgattttt gtttctaatt cattaatatg tgttcgtgtt tttaacataa
      301 ttaactcctt aagtttgttc cctattttat cacaattagg cttttttata aaaacagaac
      361 aaactatatt taatctagtc cgattctgtt ttgctggaag ttaattttac cttgattgtt
      421 tgattaatct agtactttta atgtaaatgc tttgctttca agaactttga acattgcatg
      481 tgcagtatct aaagatgtaa atagaggaac tccttgttca attgcagaac gtctgatgat
      541 gagagcatca tggttatcaa tgttattttg cccaactgtg ttgataactg cttgaatctt
      601 accattttta attaaatcag gaatatgatt tcgatcattc tctcctaact tacctactaa
      661 aactgtctct aaaccattag catcaaaata aattgcagtc ccttgggtag cataaatacg
      721 ataaccaata ctttgataga cttttgcaag ttctaaagct tcttctttgt ctgtatcatc
      781 tactgtaaaa acaatgttac catagtctgg catatggaga tatgctgctt caaaggcttt
      841 atacaaggct ttctgaagat taatatctga tcccatcact tcgcctgttg atttcatttc
      901 aggacctaat aaactatcta ctttagcaag tttggtaaaa ctaaagacag gggctttaat
      961 gtggaccata tctgaagctg gatagagatc ataaccatat gtaaaattgc agagtttatc
     1021 tcctaagata acttttgttg ctacttgggc cataggaatg tgagttactt ttgataagaa
     1081 tggaagtgtt ctaccagcac gaggattaac ttcaataaca tatactttct gatccttgat
     1141 tacaaactga atattcatca taccgataca gtttaatcca attgctaagc gttttgtata
     1201 atctacaata gtttcgataa tggtctcaga caatgtttga ggagggtata cagccatgga
     1261 atctcctgaa tgaacaccag ctcgctctat atgttccatt attccaggaa tcagtatatt
     1321 ctgaccatcg gatatagcat caacctcaca ctctttacct ataaggtatg catctataag
     1381 cactggatga tctgggcttg cctttaccgc ggttgtcata taatgacgta aatcttcttc
     1441 gttttcaact atcttcatag ctcgtccacc aagaacatat gatggtctaa ccaacacggg
     1501 ataaccaatc ttttgcgctg cattaactgc ctcttcctcc gtagtggcgg taaaacctgg
     1561 tggttgaggg atatttagag cttgtaaagt ggcttcaaat tgttttctat tttcagcacg
     1621 atccaaatct tctaattgtg tgcctagtat tttaacccct gccttgttta aatcttttgc
     1681 tagattaata gcagtctgac caccgaattg taagatgacc ccttcaggtt tttctaagtc
     1741 aataacattc ataacatctt caaaagtcaa aggttcaaag taaagcttat ctgaaataga
     1801 gaaatccgtt gagaccgtct ctggattaga gttcatgata atcgcttcaa aaccagactc
     1861 tctgatggct ttaacagaat gaaccgttga ataatcaaat tcaactccct gtccaattcg
     1921 gattgggccc gatcctaaaa ccaaaataga ggctttatct gatatcaatg actcgttttc
     1981 tacggcataa gtcgaataaa agtagggcgt tgctgaatca aactcagctg cacatgtatc
     2041 aaccatctta taaactggaa gaattcctgc ttcttttcgc atgttacgaa cttgatcttc
     2101 gtttatttgc cataaactcg ctatagcttt atctgaaaaa cctttatttt taaccttttt
     2161 gagaatattg atatccagtc tgtttttaga taatagatct tctaactcta caatcactct
     2221 taacttatca aggaagaaag aatcaatgct agtcaaactt gctaattttt caatactata
     2281 atgtctacgt aaagcttctg ctagataaaa taagcgatca tcttctgcct ttcttatttt
     2341 ttctaataaa acatcatctg atacattatc taaatctgct attttaatgt gatcgacacc
     2401 tatttctagt gaacgacaag ctttcaacaa agattcttct atattccgtc ctattgccat
     2461 gacttcgcct gtagctttca tctgtgttcc caattttcta tcgccacttt caaatttatc
     2521 aaaaggaaat ctcggcattt ttgcgactac gtaatctaaa gcaggttcaa acatagcgta
     2581 agtcgttttt gtaataggat taattacctc atctagtgtc aaaccaacag ctatcttagc
     2641 tgctaattta gcaattggat atccagtagc ttttgaagct aaagcagaag atcttgatac
     2701 tcttggatta acttcaatga catagtattt aaaactattc ggatcaaggg ctagttgaac
     2761 attgcatcct ccttcaattt tcaaagctct tataatatct aaactcgcat ctcgtaataa
     2821 ctgattttcc acatcagata acgtttgtgc gggtgcaaaa actatagagt cccctgtatg
     2881 gataccaaca ggatcaaaat tctccatatt acaaacaaca agagcgttat ctgctgcatc
     2941 acgcataact tcgtattcaa tctctttaaa gccagcaatt gaacgttcaa taaggcactg
     3001 tgtcacaggc gataacttta accccttcgt cgtgatgtca accaattgtt cttgattatc
     3061 gcacatgcct ccacctgtac ctcctaaagt aaaggcagga cgaacaatta atggataccc
     3121 gatttgctct gcaaattgaa tagcttcttc taccgaatta acaatctcgg attctggaat
     3181 aggttgattg agttcttcca tcaactgttt aaataaatca cgatcttcag ctttatcaat
     3241 ggctgataat ttggtcccta atagttcaac attcaattct tctaggatac cattttttga
     3301 tagagccata gccatattca atccagtttg acctccaaga gttggaagga gagcatcagg
     3361 tctttccttc ctgagaattc ttgtgacaaa ctccaaggta ataggttcaa tataaacttt
     3421 atcagcaata tctttatctg tcatgatagt agccggattc gaattgacca aaacaacttg
     3481 gtatccctcc tcttttaaag agagacaggc ttgtgtacca gaatagtcaa actctgctgc
     3541 ttgtccaatc acaatcgggc cagaaccgat aaccataatt ttacgtatgt ctgtgcgctt
     3601 tggcatatca aactcctcta attctatatt ttaaagctag cttttttgct ggaaatcatc
     3661 aatcatatta ataaattcat caaaaagata agatgtatca tgcggccctg gtgctgcatc
     3721 tgggtgaaac tgcactgaaa aggcaggata gtatttatgt cgaacacctt cgacggtttt
     3781 atcattaatc tcttcatgtg taatgaagag agcctcaggg aaatcttctc tcgagacagc
     3841 ataaccatga ttttgacttg taaaatcaac ctgtcctgtt tgcaaatgac gaacagcatg
     3901 gttaaaacct cgatgaccaa aagtcatttt ataagtcttt gcaccattgg ctttagcaaa
     3961 tagttgatgt cccatacaaa taccaaaaat tggaatttta ccttgaattt cttggatcat
     4021 ttgtaaagca ttcggtaatt gttctgggtt cccagggccg ttagataaga gtactccatc
     4081 tggattgaga tttaagattt cttgcgctgt cgtagtatga gggacgactg taatatgaca
     4141 ttgacgttgt gacagttctc ttagaataga atgttttaaa ccaaagtcaa ctaatacaat
     4201 atttttgcca actccaggcg aagcataagc taatttggtt gatacttgtt caatttgatt
     4261 atttaaaaga acactttttt gaaggttttc tagtgcttca tgaatagaat ttccctcgtt
     4321 tattaaacaa gctttcattg ttccatgttt tcgaataatt tttgttaatg ctcgagtatc
     4381 tatacctgaa attccaggta ttccttttaa ttttaaaaat tcatcaaatg tcatctgttg
     4441 acgccagtta cttggatact ctgcccattc ataaacaatt actcccttac atgttggtct
     4501 aatagattcg taatcgtctc gatttatccc ataatttcca ataagaggat aagtaaaagt
     4561 aatgatttga ccgttgtaag attgatctgt tatagattct tgataccctg tcattcctgt
     4621 actaaaaaca atttcaccac ttgttcctac atccgctccg aaagcctcac cctcaaacac
     4681 actaccatct tccaataata atagacgctt cattttatct cctttattat ctgctattga
     4741 gcactgcttc taagatagct atccttgcat agacgccatt actcatctgt ttcactattc
     4801 gagccttatc tgcttctact aaatcgctag caatctctac atccctattg acgggtgctg
     4861 ggtgcataat aattgctgta tcccttaaat gtttatagcg ttcttttgta agaccaaatt
     4921 gttgatgata gctttctttt gaaaacaccc ctttaccatc atgacgttcg tgttgaacac
     4981 gtaaaagcat aaggacatca atttgatcaa ctaactgatc tattggtaaa cattgcccat
     5041 attcatcgaa ctgactacta taccattctt tgggtccaga aaagaaaatt tcagctccta
     5101 atcgttttag cacttgcatg ttagatttag ctacccgtga gtgagttaga tctcctacaa
     5161 tcgcaatttt tagtcctttg aagtatccaa attcttcgta aatagttacc aaatccaaaa
     5221 gtgattggct tggatgttgg cctgagccat cacccccatt gacaatagct gaatgaatgt
     5281 tcggactagc aatcaattct ttataatagt caatatcagg gtgcctaatg acacaaacgt
     5341 ccaatcctag agcactcatt gttaagatag tgtcataaag ggtttctcct ttattaacag
     5401 aacttgtatc agcattaaat tcaattgttt ttaatcctaa tttgagttct gccatttcaa
     5461 atgacttatg tgttcttgtt gaattctcaa aaaagagatt tgacacatag taatctttat
     5521 caaaaccaat gttgctagga ttttctttaa cttcgatact tctctttatc aaagacataa
     5581 cttcttgatt tgataattct tctaaactga caaaatgttc taatgaaagt gtttgtgttt
     5641 gatgattcat gataatttct ttctataaat tttagctaga aaaaagcctc ctaaaacaag
     5701 gaggctctgc aaaatagtaa gctagatagg gaactaactt actatttgtt aacgcaattc
     5761 cttgtttgcc tctctggaca ataccttaaa ggatattaat aagaaatgtt ttacctatac
     5821 caatgtcgct gcgtggctat ctttttgata gactatttca ccattgcaaa ttgtgtagtg
     5881 aacaactccc ttcaatttat caccgataaa aggagagttg ctggatttgg aggcaaattc
     5941 tgcctttatt attctctctt cagcttgatc aaaaataacg atatctgcag gtccaccttc
     6001 tctgagataa cctgcatcaa atccatataa ctgtgcagga ttacatgtca tttttgccaa
     6061 aaagtcaatc attgagagtt ctttagtaga tactaaataa gtaatcccta aggctaatga
     6121 tgtctctaag ccagtcatac cagagggcgc ttgactaata ttatctgctg ctttctccat
     6181 aatatgatgg ggagcgtgat cacttgcaat aattgaaata acaccacttt taagaccatc
     6241 aatgagggct tgtctatcct tttcaagtcg cagtggtgga tttaattttg cattagcacc
     6301 cttagttaaa agtaagtcct ctgtctttga aaaatgttgg ggggttactt ctgctgttaa
     6361 gttagcacca agcttctgcg caaaatcaac tacttctact gattctgaac ttgacaaatg
     6421 ctggatatgg actttcgcct gtgtttggta agcaatcata gcatctcgcg caatcattga
     6481 atattcagca agaccactag ctccacagac atgataaatt ttctgagcaa tatgttcgtt
     6541 gataccaaga acaccattta acgatggatc ctcttcatgt aacgatagga caacatcatg
     6601 ttttctagct aagttcatag cttcttgcaa aaccttagta tctgtcaaag ggataccatc
     6661 gtctgagaat ccagcaactc cagcttctaa taattcttcg aaattaacta aatctttacc
     6721 attcagggat ttagtaattg tagcaactgt ttcaatctta attgcttctt ttgctgcact
     6781 ctccttaact tgttttacaa tttctgagct agaaatcgtt gggttagtat tggccatcat
     6841 aagaactgtc gtaaaaccac ctgctgcagc tgataaggct cctgtatgga tattttcttt
     6901 atgtgtctgt ccaggctcac gaaaatgtac atgaatatcg actaatcctg gagctacaat
     6961 tttattgctt gcatcaatga ttggaatgcc tttaatatca atttctttcg atatttgttt
     7021 aatttttcct tgatcaatca gcatgtctga tacttgatta aaaccacttt gagggtcgat
     7081 aatcaatccg tttttaatga tatacatatc tgtcctctct aactttgcca attcatttga
     7141 tcttctttaa attttttcag taataccagt tggtcagctg taatatatcc attaacttta
     7201 gcaactttaa ttaattgatt gtaatccgtc aaagtcacca aaggaatatc agcttcacga
     7261 aatgcttgtt cagccttagc taattgatat gtaaatatcg ctaccacccc taaaacctca
     7321 ataccttgac tctgcgcagc cgtaacagcc tctaaaacag atcctcctgt tgaaatcaaa
     7381 tcttcaatga taaccatttt ttggcctgag taaacacgtc cctcgatttg attccccaca
     7441 ccatgatcct ttgccttcga gcgtatatag gcaaagggca agttcatctt atcagctata
     7501 atagcgccat gtggtatgcc tgcagtcgct gttcctgcga tgatatcaac gtttgggaaa
     7561 tgtttctgta tttgtttaac aaaaccattt tcaatcagtg tccttgtctc aggatacgat
     7621 aaggttacac gattatcagt gtaaattggc gatttgaccc ccgaagccca agtaaagggt
     7681 tgttggggtc ttaagtaaac agcttgaata tctaatagtt ccatggcaat ctgtcttgct
     7741 aaatccatct cagtactcct ttatttaata cgattccatt cttcttttat gtctagatat
     7801 gcttgatatg gattttctgc tttcgttata ggtcttccca caacgatata atcagcccca
     7861 actatacgtg cttgctcagg agtcatcgtt cttttttgat catctaactg attggtctgt
     7921 ggaggtctga taccaggcgt taagcaaatg aaatgttcgt tagtctgttt cttgatttgc
     7981 tttacctctt gagccgatgc gactactcca tctaagcctg cttcttgagc tttttgagca
     8041 taatggcaaa ccgaatcaac tacacttaag ttaatatgtt gatccacctg catttgttct
     8101 tgtgaagtcg aggttaattg tgtcactgct aatagtattg gaccctcccc caaaccttct
     8161 ctagctgctt tcatcatttc gactcctcct gcagcatgga cattagtcat atcaatgcct
     8221 aatttggcta agacagacat agataatcga actgtattag ggatatcatg taattttaaa
     8281 tccaaaaaaa tgcgatgtcc taaggattta atatatctaa taattgaggg accttcggaa
     8341 taataaagct ccattcctat tttgacaaaa agcaattctt cttttggaaa atgttctaaa
     8401 aaagttgtta cagaagccaa atcagaaaaa tctaaggcaa tgatagggca tttttctaac
     8461 atataattcc tctctataga aataataatt gttggaaatg taccagtatg acacaaaaaa
     8521 ccttgcctga gggcaaggtt tcataaaaag ttagcgaaaa agcactcgct atttgactta
     8581 tgatggtgac cttgttcgcc tctctggact cacttaaaga tacatttgta ggtattctac
     8641 aagtttacaa tcattttgtc aaataaatac taaattattt tatcatttta cgtctaataa
     8701 agaacgatag aggaatctgc tgtacaatac tgattgctat agcaattgta aaagctgtta
     8761 caaaacagga tctaaaatat tccattgcta aggcatctgc tcccgtcaat aaaaagtaaa
     8821 cggctctata gaagctaagt cctgggataa gaggaaacat acttgtcgct aaaaagacag
     8881 tggcaggaca cttacgttta gcagctaata gccgtgaaaa ataagtgatg aaaagccctg
     8941 gaaaaaatac ggctatagca tccatgtgaa attgttgcca taagatcagg tagagcatcc
     9001 aagatattgc tcctagtatc cctaagtcaa ggaaataacg ttttggaaca tggtagagaa
     9061 tagagaaggc tatcgtccct aaaccggcca ttagtgttct gatgataatc tcaagaatgg
     9121 tattaggaac accagaaaaa gaacctgtca tttgttctgc aaacggaata atttcaattg
     9181 taatagccac gcctgctgat atagaaatac aaatgagtaa agcagacata atcaaagcta
     9241 aacctgttga aaaattattc tgtgaaaatt ctctcaccga attaacaaag gcagcccctg
     9301 gcaccataac cattaatgcc cctaatatga taatgctacg atgctcacct aatcctgaaa
     9361 aatataataa attagcactt aaagccacta cagaactacc tagaatggtc aaaagaaatc
     9421 ctgtatgtat acgactttcc atcaaattca aaaaatatcc taaaattaag cctgttacag
     9481 cagcggaaaa tgaatctaat aaggaactac ccaaggctaa agaaaaacta cccgctccac
     9541 aaaaataaga aattaaagta acaagcaaac tataatcttt ttgatgttca attaagtcta
     9601 actgctttac aatttcttct ggagatacta agtcttgttt agcaatttgc ctagaaaggt
     9661 tattaacagc ttcaagtcta cctaaatttg ctgttacacc atctgtattg catactttag
     9721 cttcttgttt accatagcgg ttaatcccgg aagcgataat actgctacta accacataag
     9781 cttcaaaatt ttctatctga agagccttag caaaatgctt cattgtgtct tcgacacgat
     9841 agacttctgc tccgctttca atcagtaatt taccagctct aagaacaacg ctcatatcat
     9901 tttgcatctt atttggctct gccttctact ttattaccct agccacttaa ctaagttggt
     9961 taaagtccca aacatcatcc atccatcctt cataaaagtc cggttcatga cataccatca
    10021 agattgatcc cttataagct tttaaagcac gttttaactc atctttagca tctacatcta
    10081 aatgattggt tggttcatca agtacaagga cattattttc gcgattcatt aataagcaaa
    10141 atctgacctt tgattgctcc ccaccagata acacctgaat ctgactttca atatgctttg
    10201 aagttaaacc acaacgagca agagctgccc taacctctgc ttggttcaaa gcaggaaaag
    10261 catcccatac tgcttcaagt ggagtttggc gattccctcc gggtacttct tgttcaaaat
    10321 aacctaaatc aataaaatca cctcgttcaa cattaccaga aatagggggg ataataccta
    10381 gtaaactctt aagtaatgtt gtttttccta tcccatttgc tccaacaata gctatttttt
    10441 gattacgttc aaatgttagg tttagaggtt ttgttaaagc acgatcatat ccaatttgaa
    10501 gatctttagc ttggaaaata aaacgtcctg gtgtgcgaga ttctttaaat tcaaaacttg
    10561 gctttggttt ctctgcttgt aactcaataa tatccatttt gtctagtttc ttttgacgag
    10621 acattgccat attacgtgtt gcaacgcgtg ctttattacg attaacaaaa tcttgtaaat
    10681 cagctatttc cttttgttga cgttcatagg ctgcttctaa ttgagccttt ttcattgcat
    10741 agactgattc aaaattagta taatcaccgg aatatcttac taaatcttga ttttcaacat
    10801 gatagacaat attgataaca tcgttcaaaa atggaatatc gtgtgagatt aaaacaaaag
    10861 cattttcata attttgtaaa tagcgtttta accaatcaat gtgttcagca tcaaggtagt
    10921 ttgtaggctc atcaagcagg aggatatctg gcttttctaa taacagtttt gctaaaagga
    10981 cctttgtcct ttgaccacca gataattcag taacatcaga atccatacca aaatccataa
    11041 ctcctaatgc ccgtgcgact tcatcaattt tagcatcaag cgtataaaaa tcacgtgatt
    11101 ctaaacgatc ttgtagttct ccaacttctt ccataaggct atctacatct gcgccatcat
    11161 ctgccatgga tatatatata tcattgatac gtgcctctgt tttaaaaagt tcatcaaatg
    11221 ctgttcgcaa aacatcacga actgtttgac cttcttctaa cacagtatgt tgatcaaggt
    11281 aaccagcagt tacatatttt gaccattcaa tttttccttc atcaggttga agatgacctg
    11341 taacaattga catgaatgtt gattttcctt caccattagc accaataagt cctatatgtt
    11401 caccttttaa aaggcgaaaa gaaacattct caaaaatagc acgatcacca aaaccgtgac
    11461 ttaagttttt tacttctaaa atactcattt aaaatccttt gattgataat agaggttatt
    11521 aaaaaactca tagagacact ctacaagttt ttaaattagc acattacata gtaagttcca
    11581 tatacaagta tagagagttt aaacttaaaa gtcaaacctt acttaatttt ttcaaagtgt
    11641 aaatgaacag tgatttgatc atcataacca tttcgctcta aatcacgttg caaacggtat
    11701 gaaatatagt gttcagcaat atgaatgtat cctgcatcca ataaacgatt ttcaaccaaa
    11761 gactgaatca tattgatcgt tggtcgttca acctgagcat cctctaaatc taagattact
    11821 tttttagtca cttgagcaag gtcttgacga agtgactcat caagcacata aactgtttga
    11881 gcagctttca agatagcttg gtagattttt tcaggatcaa attcaacaat ctgtccatca
    11941 cgtttgatga tttgcataag agcccctcct taatgtggaa acaatgttaa aacgatttgc
    12001 aatcattttc atttgttaat ttcattatgc aaatgttttc ataatttgtc aagtgtttta
    12061 cctgatattg atgaaaaatt aatatcacta gaagtcagta taagtattaa gcaatgtcat
    12121 attcttctat attaattcaa attttaactc tttcgctggt ttaaccagcc cattcttatg
    12181 caatgtttca gcgatttgaa cagaattcca agctgccccc ttcagtagat tatctgaaac
    12241 tacccacata tgaacaccat tttcttgatc aaggtctttt cgaatacgac caacaaaggt
    12301 ctccctatgg cctacagcat taatcgcttg agggtaaatt tgctgagatg gaaggtcttg
    12361 taggacggca ccaggaaaat ttgcaatagc ctttttaatt tcagagattg aggctaattc
    12421 tttcgtctca atataaatag actcagaatg acccgataaa acgggaatgc ggacgcaggt
    12481 agctgaaacc ttaatagtag catcttccat aattttcttt gtctccaaag tcattttcat
    12541 ttcttcgtac gtataatcat tatcagtaaa aatatcaatt tgaggtaatg cattgaatgc
    12601 gataggataa tgtttttgat cgctagaaca tggcaagacc gttgcaatta attgatcagg
    12661 tgaaaggtta tcatttaaaa cttgtctcaa ctgttcctta gtttcttcaa cagcacgtgc
    12721 acctgaaccc gaaacagctt gataggtgga aactataaca cgctctatcc cccatttttg
    12781 acgaatgggc tctaaagcaa tcatcatttg aatagtagaa caattgggac aagctatgat
    12841 accattatga ccaatcatgg catgagcatt tacttcagga acaactagtg gaacatcagg
    12901 attctgacga aaatatgacg tgttatctac tacaactgct ccagacttta ctgcataagg
    12961 agcgaacttt gctgaaatag atccaccagc tgaaaacaag gcaatatcaa catcgtaaaa
    13021 tgattctttt gttgtctctt caacacgtat agcctcatct ttaaaatgta aaattttacc
    13081 tgctgagcga cttgatgata aaagtttcac ttgttctatt ggtaaattcg attgttctaa
    13141 ttgacgaatc atttgtgttc ctacggcacc tgtagcaccc acgatagcaa cggtatatcc
    13201 catataatga cctctatttg ttgatgttat tcatcaatat tttcaaaaca ttttaatgtg
    13261 actattatac tacattttta taaactttca agaatagcca aatgaaaaaa gtcaccctaa
    13321 ccaacctaat tataaaaagg aatctcgtat ccgattcc
//
LOCUS       DATRQA010000048        13343 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.048, whole genome shotgun sequence.
ACCESSION   DATRQA010000048 DATRQA010000000
VERSION     DATRQA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.048"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1003)
                     /locus_tag="VBD27_001015"
     CDS             complement(<1..1003)
                     /locus_tag="VBD27_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEO7025039.1"
                     /translation="MSNFYKLLKEKFPRKEDIVTEMINLEAICQLPKGTEYFISDLHG
                     EYDAVDYLLRTGAGSIRAKLLDCFDWQKIVAVDLDDFCILLYYPKEKLAFDKMNLSVS
                     AYKTKLWEMIPLQIQVLKYFSSKYTKSKVRKQLPGKFAYIIEELLAEIDRNPEKKSYF
                     DTIIEKLFELDQVEDLIIVLSQTIQVLIIDHLHVVGDIYDRGRYPDRILNRLMAFPNL
                     DIQWGNHDVTWMGAASGSYLCMVNVIRIAARYNNITLIEDRYGINLRRLVDYSRRYYE
                     PLPSFAPILDGEEMTHPDELDLLNMIQQATAILQFKLEAQLIDRRPEFQMHNRQLINQ
                     "
     gene            1155..2015
                     /locus_tag="VBD27_001016"
                     /pseudo
     CDS             1155..2015
                     /locus_tag="VBD27_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(2060..4744)
                     /locus_tag="VBD27_001017"
     CDS             complement(2060..4744)
                     /locus_tag="VBD27_001017"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEO7025040.1"
                     /translation="MLKEQKKSLFYTQGQEEVLTSLESSREGLSTTEAKNRLEMYGRN
                     ELEEGKKRSLIAKFFDQFKDLMIIILLVAAALSVITEGMHGLTDALIILAVVILNAAF
                     GVYQEGQAEAAIEALKDMSSPIARVRRDGHTIEVDSKELVPGDLVMLEAGDVVPADLR
                     LLEAASLKIEEAALTGESVPVEKDISQVVAEDAGIGDRVNMAYQNSNVTYGRGYGVVT
                     NTGMYTEVGKIADMLANADESETPLKQSLVQLSKLLTYLIVIIAVITFLVGIFVRKEG
                     WIEGLMTSVALAVAAIPEGLPAIVTIVLSMGTKTLAKRNSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGVLQSSSEEISVDNNTLRIMNFSNDTKIDPSGKLIGDP
                     TETALVQFGLDKNFDVREVLKNEPRVAELPFDSDRKLMSTIHKESDGRYFIAVKGAPD
                     QLLKRVTKIEDNGLVRDITAEDKEAILNTNKELAKQALRVLMMAYKYETQIPTLETDI
                     VESDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDA
                     NDTEDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKS
                     IQYLLSANMAEVFTIFFATLLGWDVLAPVHLLWINLVTDTLPAIALGVEPAEPGVMTH
                     KPRGRQSNFFDGGVMGAIIYQGILQTILVLGVYGWALMYPEHAGYRMIHADALTMAFA
                     TLGLIQLVHAFNVKSVYQSIFTVGAFKNRTFNWSIPVAFILLMVTIVVPGFNKLFHVT
                     HLSSTQWLTVVIGSLLMVVLTEIVKFIQRKLGQDEKAI"
     gene            complement(5088..5486)
                     /locus_tag="VBD27_001018"
     CDS             complement(5088..5486)
                     /locus_tag="VBD27_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEO7025041.1"
                     /translation="MREVMKLEINNNIQIDNETEMIHEIHDCQFIEKGSYVYLNYINA
                     EGERVVIKANHEELLMTRFSNPKSVMRFHRETPALVNIPTPLGIQHLITETSHYQFDL
                     SQQRLHINYVLKQTETGACFANYELKIQWY"
     gene            5550..6866
                     /locus_tag="VBD27_001019"
     CDS             5550..6866
                     /locus_tag="VBD27_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7025042.1"
                     /translation="MNEKVFRDPVHTYIHVNNQVIYDLINTKEFQRLRRIKQTSTTSF
                     TFHGAEHSRFSHCLGVYELARKVTEIFDEHYSDLWNKNESLLTMAAALLHDIGHGAYS
                     HTFERLFNTDHEAYTQEIITNPTTEINAILRKVAPDFPDKVASVINHSYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYYTAASYGQFDLTRILRVIRPTDSGIAFARNGMHAVEDYIVS
                     RFQMYMQVYFHPASRAMELLLQNLLKRARFLFDTHRDFFEQTSPNLIPFFTDQYDLQD
                     YLALDDGVMNTYFQSWMQADDNILADLANRFINRKVFKSITFEEADKENLVKMKELVS
                     QVGFDPDYYTGVHANFDLPYDVYRPEHSNPRTEIQIIQKNGQLAELSSLSPIVKALTG
                     SNYGDQRFYFPKEMLTLDSLFSSTKEEFQSYITNEHLTLTKDNSHS"
     gene            6938..7750
                     /gene="yidA"
                     /locus_tag="VBD27_001020"
     CDS             6938..7750
                     /gene="yidA"
                     /locus_tag="VBD27_001020"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEO7025043.1"
                     /translation="MTSIKLVAVDIDGTLLNSKREITPEVAKAVQEAKSKDVKIVIAT
                     GRPIIGVQDLLEELKLNEEGDYVITFNGGLVQDTATGDDIIKETLTYEDYLDFELLAR
                     KLGVHMHAITKEGIYTANRDIGKYTIHEATLVNMPLFYRTPEEMGDKEIIKLMMIDQP
                     DILDAAIAKIPKKVLDNYTIVKSTPFYLEILPKNVNKGTALLHLAEKMGLTVDQTMAI
                     GDEENDRAMLEVVGNPVVMQNGNPELKKIAKYITKSNEESGVAYALREWVIN"
     gene            7735..8965
                     /locus_tag="VBD27_001021"
                     /pseudo
     CDS             7735..8965
                     /locus_tag="VBD27_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
     gene            8978..10189
                     /locus_tag="VBD27_001022"
     CDS             8978..10189
                     /locus_tag="VBD27_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7025044.1"
                     /translation="MYREITAVEHDRFVSESNQTNLLQSSNWPKVKDNWGSQLLGFFD
                     GETQIASASILIKSLPLGFSMLYIPRGPIMDYSNLDIVTKVLKDLKAFGKKQRALFIK
                     CDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPRFQANLY
                     ANQFGLDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDY
                     YQKLLDTYPNNSYITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQDTI
                     DRLNKEIDFLKEAQKAYDRDIVPLAATLTLEFGNTSENIYAGMDDYFKSYSAPIYTWF
                     ETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNRLLYKASN
                     YVYALRKKRNS"
     gene            10208..11443
                     /locus_tag="VBD27_001023"
     CDS             10208..11443
                     /locus_tag="VBD27_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7025045.1"
                     /translation="MALKELTAKEFESYSGNYDLQSFMQTPEMAKLLKKRGYDITYMG
                     YQIDGKMEIISIVYTIPMTGGLHMEVNSGPAHSNSKYLKHFYKELQNYAKSQGALELL
                     IKPYDTYQEFTGEGEPKGAPNTYLIDGLTSIGYHHDGLHIGYPGGEPDWHYVKNLEGI
                     TPQNLLKSFSKKGRPLVKKAMSFGIKIRVLKREELHIFKDITSSTSDRRDYMDKSLDY
                     YQDFYDSFGDKAEFVIATLNFREYDHNLQLNAKKLEEQITVLDNRHQNNTDSAKYHRQ
                     RTELVNQLASLDKRRKEVEPFIQKFGNQDVVLAGSLFIYSPKETVYLFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRQSTFYNFLGIQGNFDGSDGVLRFKQNFNGYIVRKMGTFRYY
                     PNPLKYKSIQLLKKILRRT"
     gene            11631..12563
                     /locus_tag="VBD27_001024"
     CDS             11631..12563
                     /locus_tag="VBD27_001024"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEO7025046.1"
                     /translation="MVSLKTEIAGFSFDNCLMNAAGIYCMTKEELLAIENSEAGSFVT
                     KTGTLKAREGNPQPRYADTDWGSINSMGLPNKGIDYYLDFVTELQDQDNSKNHVLSLV
                     GLSPEETHIILKKVENSSYNGLIELNLSCPNVPGKPQIAYDFEMTDLILSEIFSYYQK
                     PLGIKLPPYFDIVHFDQAATIFNKYPLAFINCVNSIGNGLVIDDETVVIKPKNGFGGI
                     GGDFIKPTALANVHAFYKRLNPSIKIIGTGGVKNGRDAFEHILCGASMVQIGTALQKE
                     GPEIFQRVSRELKEIMADKGYQSLEDFRGQLNYL"
     gene            complement(12607..12804)
                     /locus_tag="VBD27_001025"
     CDS             complement(12607..12804)
                     /locus_tag="VBD27_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025047.1"
                     /translation="MSEKAILLIILIAFVIIAIIKIALLKLYMNTIKPKVDNEGNIIT
                     KPVKDDLTPEEREEREFDKDW"
     gene            complement(13180..>13343)
                     /locus_tag="VBD27_001026"
     CDS             complement(13180..>13343)
                     /locus_tag="VBD27_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000965644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO7025048.1"
                     /translation="SGDTVAIVRKNGKIFDYVLPHEKVRDDEVVAVESVEDVLAELGN
                     VRCDISSCQ"
BASE COUNT         4446 a         2537 c         2178 g         4182 t
ORIGIN      
        1 cctggttaat aagttgtcga ttatgcattt gaaattctgg acgcctgtca atcaattggg
       61 cttccaattt aaactgtaaa attgctgttg cttgttgtat catatttaac aaatctaatt
      121 catctggatg agtcatttcc tctccatcta atatgggggc gaaagaaggt agaggttcat
      181 agtaacggcg tgagtaatca accaagcgac gcaaattgat accatatcgg tcttcaatta
      241 aagtaatatt attataacgt gcagcaatac ggataacatt taccatacaa aggtaggaac
      301 cagatgctgc tcccatccaa gtgacatcat ggttccccca ttggatatcc aaatttggaa
      361 aagccattaa cctattcaag atacgatcag gatacctacc acgatcgtaa atatccccta
      421 caacatgtaa atggtcaatg attaaaactt gaattgtttg ggataatact ataattaaat
      481 cttctacttg gtccaattca aataattttt caataatagt atcaaaatag cttttcttct
      541 ctgggttacg gtcaatctca gctaaaagtt cttcgataat gtaagcaaac ttacctggca
      601 attgcttacg tactttagat tttgtatatt tactagaaaa atattttaaa acttgtattt
      661 gtaacggaat catttcccat aacttcgttt tataagcact tactgacaag ttcattttat
      721 cgaaggctaa tttttccttt gggtaataaa gcagtataca aaaatcatct aaatcaacag
      781 caactatttt ctgccaatca aagcagtcta ataatttagc tctaatagac cctgcacctg
      841 tccgtaagag ataatcaaca gcatcgtatt ccccgtgtaa atcactaata aaatattccg
      901 tccctttggg taattgacaa atagcttcta aattgatcat ttctgttacg atatcttctt
      961 ttcgaggaaa tttttctttc aagagcttat aaaaattaga catgagacct ccttaaggca
     1021 ccttaaatat aaagcttcta aacacaatta tgaaagcgca ttctacaatg acatatctat
     1081 tatagaatga ctttaaaact atttcaaaaa tttactgttt atctctttga actacaatat
     1141 ggtacaatac ccttatgacc aaacttgcta tcatgagtga tttacatatt gacctcaatc
     1201 atttttctac ctttgagacg aaaacgttaa ttaatttgct taagaaagaa aaaattgatc
     1261 acctccatat cgctggggat atatctaatc attttactaa agatacactt ccctttatca
     1321 ataacttaaa aaagcatatt aaacttagct ataatttagg aaaccatgat atgcttgatt
     1381 taacagaaac agaaatccaa cgccttgact ttcaaaccta tcgatttgac aaaaaaatgc
     1441 tactagcttt tcatggctag tatgattaca gcttttcaaa taatagagat atcaaggatg
     1501 ttgaaaaatt aaaaaagact ttttggtttg atagacgttt aaaacgtcca aataatgatg
     1561 taactatcca agcatcaatt ttaaaacgct tagacgaaat attagcaaaa gtcgattctt
     1621 caaatatcat tattgctatg cactttgttc cccataaaca gttcacgatg actcatccta
     1681 gattctctcc ttttaatgct tttttgggga gtcaggctta tcatgattta ttccaaaaat
     1741 atcatatcaa agatgttgtt tttggtcatg cacaccgctc atttggcgac gttaaaattg
     1801 gagagactac ttatcattct agaccacttg gatatattag agaatggaat ttaactattg
     1861 actttgtcaa tcagaatccc aatcataacc ctaatttaac ctggaatctt agcaaaaggc
     1921 ataatgccgt taagcatttg gatagttttg aaaactatcc aaaaaaatat tttgaagacg
     1981 aattacgcaa ctctatgaca atatttgatt gctaaaaaac tcacttagat attctaagtg
     2041 agttttttta tcagctaaac taaatagctt tttcgtcttg tcctaattta cgttggataa
     2101 atttaacaat ttccgttaac actaccatca ataaacttcc gataactaca gttaaccatt
     2161 gtgttgaact taaatgagtt acatggaaaa gtttattaaa tccaggaaca acaatagtca
     2221 ccatcaaaag aatgaaagct actggaatag accagttaaa ggttctattt ttaaaagctc
     2281 caactgtaaa gattgattgg taaactgatt taacgttaaa tgcatgtacc aattggatta
     2341 aaccaagcgt tgcaaaggcc atcgtaaggg catctgcgtg aatcatgcga taaccagcat
     2401 gttctggata catcaatgcc caaccataga caccaagtac taagatagtt tgtaaaatac
     2461 cttgatagat aatagctccc ataacaccac catcaaagaa gtttgactgg cgaccacgag
     2521 gtttatgtgt cataactcct ggttcagctg gctcaactcc aagagcgata gctggaagag
     2581 tatctgttac caagttaatc caaagaaggt gaaccggtgc aagtacatcc catcctaata
     2641 aagttgcaaa gaaaattgta aatacctctg ccatgttggc tgaaagaaga tattgaatag
     2701 atttttggat atttgagaag actttacgtc cttcctcaac agccacaata atagttgcaa
     2761 aattatcatc agcaagaacc atatcagaag cacctttgga aacttctgta ccagttattc
     2821 ccataccaat accaatatct gctgttttta gcgatggagc atcattaaca ccatcaccag
     2881 tcatggcaac gactttgcca tcgttttgcc aagctttcac gatacgcact ttatgttcag
     2941 gagaaacacg tgcatagaca gaatattgtt taaatacttt ttgaaactct tcgtcactta
     3001 attcattaag ttcagcaccc gtaaagacat gatcttctgt gtcatttgca tcaataatac
     3061 ctagacgctt tgcaatagct tctgcagtat cttgatggtc cccggtaatc ataatcggac
     3121 gaattcctgc ttctttagca actcgaacag cttctgccgc ttctggacgc tcagggtcaa
     3181 tcattcccac taaacctgag aatactaaat ctgactcaac aatatcagtc tctaaagttg
     3241 gaatttgtgt ttcatattta taagccatca tcaagacacg aagagcttgt tttgcgagtt
     3301 ctttattggt atttaggata gcctctttat cttcagccgt gatatcacga accaagccat
     3361 tatcttcaat tttagtaact cgttttaata attgatcagg agcacccttg actgcgataa
     3421 agtagcgacc atctgattct ttatgaatcg tagacattaa cttacgatct gaatcaaatg
     3481 gtaactcagc gacacgtggt tcatttttca aaacttcacg gacatcaaaa ttcttatcta
     3541 aaccaaattg taccagtgca gtctctgttg ggtcaccgat taattttcct gaaggatcaa
     3601 tcttagtatc gttggagaaa ttcataatac gtaaagtgtt attatcaact gaaatttctt
     3661 ccgaagatga ttggagaacg ccattagtat acactttttc gacagtcatt tgattcattg
     3721 tcagagtacc agtcttatcc gatgcaatga tttctgtaga acctaaagtt tcaactgcag
     3781 ggagtttacg aacgatagaa ttacgtttag caagagtttt tgttcccatt gataagacaa
     3841 tcgtaacaat tgcaggaagc ccttctggaa tagctgcaac tgctaaagca actgaagtca
     3901 tcaatccttc aatccaacct tctttacgga caaatatccc cactaagaaa gtaataaccg
     3961 caataatcac gattaagtaa gtcaataatt tagaaagttg aactaaactt tgtttcaatg
     4021 gtgtttcaga ttcatcagca tttgccaaca tatcagcaat tttaccaact tcagtataca
     4081 tccctgtgtt agtaacaaca ccatatcctc gaccatacgt gacatttgaa ttttgatacg
     4141 ccatattcac acgatcacca atacctgcat cctctgctac tacttgagat atgtctttct
     4201 caacaggtac tgactcacca gttagagctg cttcttcaat ttttaaagac gcggcttcta
     4261 aaagtcgtaa atctgcggga acaacatctc ctgcttctaa cataacgagg tcaccaggaa
     4321 ctagttcttt agaatccacc tcaatagtat gaccatcacg acgaacacgc gcaattgggc
     4381 ttgacatatc tttgagggct tcaatagctg cttcggcttg cccctcttga taaacaccaa
     4441 aagctgcatt taagataaca actgccaaga taatcaatgc atccgttaag ccatgcatac
     4501 cttccgtaat aacagagaga gctgcagcaa ccagtaagat aataatcatt aaatctttga
     4561 attggtcaaa aaacttagct attagactac gttttttccc ttcttcaagc tcattgcgac
     4621 catacatctc taagcgattt ttcgcttctg ttgttgataa tccttcacga gaagattcta
     4681 aagacgttag aacctcttct tgaccttgtg tgtaaaacaa ggattttttc tgttctttta
     4741 acaaaacctt tcctcctttg gtctctccac aaacaaaaag agacctgttt ttatgaaaac
     4801 aagtctcgct gtttaagaca acgccggaaa tttctttcgt aatgacgaca ttgccacggt
     4861 aaaccgtctg ctactccctt gatatgttaa tcattgtaac agaaaaaaat ccatttttca
     4921 attaattatc cttgctatca ctaataatct caagggaaaa aacgaagttc atctagtcga
     4981 aataaaccat actgcagttt atcaaataaa tggctctcat aaagagatcc cagcgtcttc
     5041 agtagacgaa attcataatg actactcttt tgttaatggt aattatctca ataccattga
     5101 atctttaatt cataatttgc aaaacaggct cctgtctccg tttgcttaag gacataattg
     5161 atgtgcaacc tttgctgtga caaatcaaac tgatagtgac ttgtctctgt aatcaaatgt
     5221 tgaataccaa gtggagtggg gatattaacc aaagcaggcg tttcacgatg aaaacgcatt
     5281 acagacttag gatttgaaaa cctcgtcata agcaattcct catgatttgc cttaataact
     5341 actctctcgc cctcagcatt aatataattc aaataaacgt aacttccttt ttcaataaat
     5401 tgacaatcgt gaatctcatg aatcatttcg gtttcgttgt caatttgaat gttattgtta
     5461 atttctagtt tcatgacttc tctcacttct tttatccttc tattctacca atttctgact
     5521 caagtttcaa tttttgctat aatatatata tgaatgaaaa agtatttcgt gaccccgttc
     5581 acacttatat ccatgtaaat aatcaagtca tatatgactt gattaacact aaagaatttc
     5641 aacgtttacg acgcattaaa caaacctcaa cgacttcttt taccttccat ggtgcagagc
     5701 atagtcgttt ttcacattgt ctcggagttt atgaactggc tcgtaaagtt acagaaatct
     5761 ttgatgaaca ttattctgat ctttggaata aaaatgagtc cctgctaacg atggctgccg
     5821 ccctactaca tgatatcgga catggcgcat attctcatac atttgagcgt ctcttcaata
     5881 ctgatcatga agcttacact caggaaatca tcaccaatcc taccacagag attaatgcta
     5941 ttttacgaaa ggtggctcct gattttcctg ataaagtagc tagtgttatt aatcatagct
     6001 accccaataa acaagttgtt caattaattt ctagtcaaat tgattgtgat cgaatggact
     6061 atcttctcag agattcttac tacactgctg ctagctatgg acaatttgat ttaacacgaa
     6121 ttttacgcgt tattagacca accgattctg gaattgcttt tgctcgtaac gggatgcacg
     6181 ctgtggaaga ttatattgtt agtcgcttcc aaatgtatat gcaagtttat ttccatccag
     6241 ccagtcgtgc tatggaatta ctcttacaaa atttactaaa acgtgctcgc ttcttatttg
     6301 atacccatcg cgattttttt gaacaaactt ctccaaatct tattcctttc ttcacggatc
     6361 aatatgattt acaagattat ttagccctag atgatggggt aatgaatact tacttccaat
     6421 cttggatgca agctgatgat aacattttgg cagatttagc taatcgtttt atcaatcgaa
     6481 aagtctttaa atcaattacc tttgaagagg ctgataaaga aaatctagtt aaaatgaaag
     6541 aactagtctc acaggttggt tttgatcccg attattacac tggtgtccat gctaattttg
     6601 acttacctta cgatgtttat cggcctgaac attcaaatcc acgaacagaa attcaaatta
     6661 tccaaaagaa tggacaactt gctgaattat caagcttatc accgattgta aaagcattaa
     6721 ctggatctaa ttatggtgat caacgttttt attttccaaa agaaatgttg acattagata
     6781 gcctattttc aagtacaaaa gaagaatttc aatcttatat cacaaatgag catttgacac
     6841 tcacgaaaga taatagccat tcatgacaaa ccctttattt tttagtaaga tataaggtga
     6901 gtattttagg atattgaaat aaagagtggg agataaaatg acttcaatta aacttgttgc
     6961 cgtagatatt gacggcaccc tattaaatag taagcgtgaa attacacctg aagtggctaa
     7021 agctgttcaa gaagcaaaat caaaagacgt taaaattgtt attgctacag gacgtccaat
     7081 catcggagtc caagatcttt tagaagaatt gaaactcaat gaagagggag attatgttat
     7141 cacctttaac ggcggtctag ttcaagacac agccacagga gatgacatca tcaaagaaac
     7201 cttaacctat gaagactatc tggatttcga actgttagcg cgtaaattag gcgtccacat
     7261 gcacgccatt acaaaagaag gaatttacac agcgaatcgt gatattggta aatatacaat
     7321 acacgaagca actttagtta atatgccgct cttctatcgt actcctgaag aaatgggaga
     7381 caaagaaatc attaaactaa tgatgattga tcaacctgac atcttagatg cagcaattgc
     7441 taaaattcca aaaaaagttc tcgataacta caccattgta aaatcaactc ctttttattt
     7501 agaaatttta cccaaaaacg ttaataaagg cactgcactt ctccaccttg cagagaaaat
     7561 gggattaact gtagatcaaa caatggctat tggtgatgaa gaaaatgacc gtgctatgct
     7621 tgaagttgtt ggaaatcctg ttgttatgca aaacggcaat cctgaactaa aaaaaatagc
     7681 aaaatatatc accaaatcaa acgaggaaag tggcgtagcc tacgcactac gtgaatgggt
     7741 aataaactga tgtatcatgt taccgttgga atatccgaaa aagaatatga tgcctttgct
     7801 attgcatcgt ctcaaaccaa tctcctccac tcttcaaagt gggcacaagt aaaatcaaat
     7861 tggcaaaatg aacgtttagg cttttataaa gatgaccaat tagttgctgt agctagcatc
     7921 ttaattaaat cattacctct tggttttaca atgctttata ttccacgtgg acctattata
     7981 gactacagca ataaagaatt agttggcttt gtgttaaaaa cactaaaaaa ctttggacga
     8041 aagaaacgtg ctgtttttgc aaaattcgat ccagcacttc ttctacgtca atatcacctt
     8101 aaagaagaga atgtcgccga agagatagat gaaagtagac aggcaattga taatcttaag
     8161 tcagcaggtg cccaatggat aggtccaacg aaagccattt ccgaaaccat ccaaccacgt
     8221 tttcaagcga acatctatac caaagcaaat atagaagaaa actttccaaa acacactaaa
     8281 cgtctgataa aagatgcaaa gcatcgcggt gttcaaattt atcgtgccaa cattgatgat
     8341 ttacctaaat tcgccacggt tgttaaccga gaatcgtaaa ggtgtagctt tacgtaatga
     8401 aaactatttc catcaactaa tgacaattta tggagaagat gcctatctct atttggctaa
     8461 agtcaatctt cctaaaagat tagcgcaatt taaagaacaa cttcttcaaa tccaaagaga
     8521 tttatcagaa actccatccc atcaaaaaag tcgcctgaca aggctaaatc aacaagaagc
     8581 ttctgttaaa caatatatcc ttgaatttca ggagtttagc aagaaatatc cagatgagcc
     8641 tgtcattgca ggaattttgt ccattcgttt tggcaatgtc ctggagatgt tgtatgctgg
     8701 tatggatgat agcttccgta aattttatcc acagtatctc ttgaatgcta gggtgttcga
     8761 agatgctttc aaaaacggta ttgtatctgc taatctaggc ggtgtcgagg gaagtcttaa
     8821 cgatggttta acaaaattta aaagtaactt caatccaatg tttgaagaat atattggcga
     8881 gtttaatcta gcaatcgatc cattattata taaattggcc aacctagcct atactattcg
     8941 aaaaaaacaa agacactctc actaagaaag gaaactcatg tatagagaaa ttaccgctgt
     9001 cgaacacgat cgctttgtga gcgaatccaa ccaaacaaac ctacttcaat cttctaattg
     9061 gcccaaagta aaagacaact ggggtagtca attacttggc ttttttgacg gtgaaaccca
     9121 aattgccagc gctagtattc tcatcaaatc acttcctctt ggcttctcca tgctgtatat
     9181 tccgcgtgga ccaatcatgg attactccaa tctagatatt gtaactaagg tccttaagga
     9241 ccttaaagct tttggcaaaa aacaaagagc tctctttatc aagtgtgatc ctctcatcta
     9301 tttaaaaatg gtcaatgcca aagattttga aaattcgccc gatgaaaaag aagggttaat
     9361 cgccattgat catttacagc gtgcaggtgc tgattggact ggtcggacaa cagatttagc
     9421 tcatactatc caaccacgtt ttcaagcaaa cctatatgct aatcaatttg gacttgataa
     9481 aatgtctaaa aaaactcgtc aagctattcg aacttctaaa aataagggag tagatataca
     9541 atttggtagc catgaactac ttgaagattt tgcagagtta atgaaaaaaa cagaagaccg
     9601 taaagggata aatcttagag gaattgacta ctaccaaaaa ctccttgata cgtatcctaa
     9661 taactcatac atcacaatgg cttcgctaga tgttgcaaaa cgattagaaa aaatagaaaa
     9721 agaatgccag atagctcaat ctgaaagaat aaaatcactt gaacttaatc gtgagaaaaa
     9781 agttaaacaa caccaagata cgattgatcg attaaataaa gaaattgatt ttctcaaaga
     9841 agctcaaaaa gcctatgacc gagacattgt tccattggct gcaaccctca ctctagaatt
     9901 tggtaataca tcagaaaata tttatgctgg tatggacgat tacttcaaat cttattctgc
     9961 tcctatttac acttggtttg aaacagctca aagggctttt gaacgtggaa atatttggca
    10021 aaatatgggg gggattgaga atgacttatc tggtggttta tatcatttta agtctaagtt
    10081 cgaaccaatc attgaggaat ttattggaga atttaacatt ccagtgaaca gattgcttta
    10141 taaagcctct aattatgtct atgccttacg caaaaaacgt aatagctagt taggaaagga
    10201 tacttttatg gcgcttaaag aattaacagc taaagagttc gaaagttatt caggaaatta
    10261 cgatttacaa tcgtttatgc aaacccctga gatggcaaag cttctaaaaa aacgtgggta
    10321 tgacattacc tacatgggct accaaatcga tgggaaaatg gaaatcatca gtattgttta
    10381 taccatccca atgactggtg gacttcatat ggaagtcaat tctggtccag cccattccaa
    10441 ttctaaatat ctgaaacatt tttataaaga actacagaac tatgccaaat ctcagggcgc
    10501 tttagaatta ttaattaaac cttatgatac ctaccaagag tttactggtg aaggtgaacc
    10561 taaaggtgca ccgaacacct atcttattga tggcctgact agtataggtt atcaccacga
    10621 tgggcttcat attggttatc ctggcggtga acctgattgg cattatgtta aaaatctaga
    10681 aggtatcact cctcaaaatc ttcttaaatc tttctctaaa aaaggacgcc ccttggttaa
    10741 gaaagccatg tcttttggta taaaaatacg tgtcctcaag cgggaagaac tacatatctt
    10801 taaggatatt acttcttcta cttctgatcg ccgggactat atggataaat ctctagatta
    10861 ttatcaagat ttttatgata gtttcggaga taaggcggaa tttgtcattg caactctaaa
    10921 ctttagggaa tatgatcata acctacaatt aaatgcaaag aaattggaag aacagattac
    10981 agtccttgat aatcgccatc aaaataatac agattctgcc aaataccatc gtcaacgtac
    11041 tgagcttgtc aatcaactag ctagtttaga taaacgtcgc aaagaagtcg aaccttttat
    11101 tcaaaaattt ggaaatcaag atgttgtttt agctggtagc ctctttattt atagccctaa
    11161 agaaactgtc tatcttttct ctggctcata cacagaattt aataaatttt atgcgcctgc
    11221 agttcttcaa gaatatgtta tgcaagaggc tctaaagcgt caatcaactt tctataattt
    11281 tttaggtatt caaggaaatt ttgatggttc agatggcgta cttcgcttta aacagaattt
    11341 taatggttac attgttcgta aaatgggtac tttccgctac tatccaaatc cattaaaata
    11401 taagagtatt caattactaa agaaaatctt acgccgaaca taaaaaataa gcttacataa
    11461 gcttattttt ttatgcgtta tagaaagcaa catttatttc tttactatta ttgtttacac
    11521 aaataataac gttcgttatt tagcgaaatt ttattaccaa tatcattatt ttcttgccgc
    11581 tctgttaatc aagtgataaa atagtaaaaa tttatcttca ggaggatatt atggtatcgc
    11641 taaaaacgga aattgctggt tttagttttg acaattgttt gatgaatgca gcgggtattt
    11701 attgtatgac taaggaagag ttattagcta ttgaaaattc tgaagctggt tcctttgtta
    11761 caaaaacagg tacccttaaa gctagagagg gaaaccctca accacgttat gcagatactg
    11821 actggggatc aattaattct atgggactcc ctaataaagg tattgactat tatcttgatt
    11881 tcgtgactga attacaagac caagacaata gcaaaaatca cgttttatca ttagtaggtt
    11941 tatcaccaga agagacacat attattctta aaaaggttga aaacagtagt tacaatggcc
    12001 tgatagagtt aaatttatcg tgcccaaatg ttcctggaaa acctcaaatt gcttatgatt
    12061 ttgaaatgac tgacctcatc ctttcagaaa tctttagcta ttatcaaaag ccattgggca
    12121 tcaaattacc accttacttt gatatcgttc actttgatca agcagctaca atttttaaca
    12181 aatatccttt agcttttatt aactgtgtta attctatcgg aaatggactt gttattgatg
    12241 atgaaactgt cgttataaag cctaaaaatg gctttggagg tatcggcggt gattttatca
    12301 agcctaccgc cttagctaac gtccatgctt tctacaaacg tttaaatcct tcaattaaaa
    12361 ttatcggaac aggtggtgtt aaaaacggcc gtgatgcctt tgaacacatt ctatgtggag
    12421 ctagtatggt ccaaatcgga accgctttac aaaaagaagg accagaaatt tttcagcgtg
    12481 taagcagaga gttaaaagaa ataatggctg ataaaggcta tcaatcattg gaggattttc
    12541 gaggacaact taattacctt taaaaataat aaaagagcaa accttgtttg ctcttttata
    12601 ttgattttac caatctttat caaattcacg ttcttcacgt tcctcgggtg ttaaatcatc
    12661 ctttacaggc tttgttatga tgtttccttc attatctact ttaggtttta tcgtattcat
    12721 atataacttt aacagagcta tctttataat agcgataata acaaaagcaa taaggataat
    12781 taatagtata gctttttctg acattctctt tcgcaacctc tcttttaact tatcttttag
    12841 gaattataac atcacagtta agataattca agggtatgct ctaacacatt tgaaactcta
    12901 aattcctttg agctctctag tcgtttattg agtattggat ataaaaaaga accttgtcgc
    12961 tatcaataaa cattgatatg acaaggtttt tctagacgaa ttatttaaca gcgtctttaa
    13021 gagttaggtt tttcggttgg cattataagg tctaaaacat atctatataa gtcgttatga
    13081 gtagtaaaca taaaagtcgt atcattgtta acatagatta gttttaaact tatgccccaa
    13141 attcgcccca caaaaaagta tattgttagt tggggcaaac tattgacagc tacttatatc
    13201 acaacgaaca ttacccagtt ctgcgagtac gtcttccaca ctctctactg ccacaacttc
    13261 atcatctctc actttttcgt gtggtaacac ataatcaaat atctttccgt ttttacgcac
    13321 tatcgctact gtgtcccctg aaa
//
LOCUS       DATRQA010000049        13210 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.049, whole genome shotgun sequence.
ACCESSION   DATRQA010000049 DATRQA010000000
VERSION     DATRQA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.049"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            28..780
                     /locus_tag="VBD27_001027"
     CDS             28..780
                     /locus_tag="VBD27_001027"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEO7025049.1"
                     /translation="MATIMLNRPDISNGFNIPMCQEIIDAIRLMSENKDVMFLVIEAQ
                     GPIFSIGGDLKVMKDAVESDDISSLTKIAELVNQISYDLLQLEKPVVMCVDGAVAGAA
                     ANIALAADFVIASKKSKFIQAFVGVGLAPDAGGLLLLSKSIGITRAVQLALTGESLSA
                     EKAEALGIVYKLCESDKIGKIKDQLLKRLSRHSINSYQAIKSLAWEAAFKDWEQYKKL
                     ELQLQESLAFKQDFKEGVRAHADRRRPNFLGK"
     gene            876..1310
                     /locus_tag="VBD27_001028"
     CDS             876..1310
                     /locus_tag="VBD27_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7025050.1"
                     /translation="MEYDQINSYLVDIFNRIMIIEEMSLKTSQFSDVSLKEMHTIEII
                     GKHSEVTPSDVARELMLTLGTVTTSLNKLEKKGYIERKRSSIDRRVVHLSLTKRGRLL
                     DRLHSKFHKSMVSHIIEDLGEEDIKMLTSALGNLHKFLEDLV"
     gene            1310..2281
                     /locus_tag="VBD27_001029"
     CDS             1310..2281
                     /locus_tag="VBD27_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO7025051.1"
                     /translation="MVFAKISQLAHYAPSQIIKNEDLSLIMDTSDDWISSRTGIKQRH
                     ISKNETTADLANKVAEQLIEKSGYSASQIDFIIVATMTPDSMMPSTAARVQAHIGASN
                     AFAFDLSAACSGFVFALSTAEKLISSGSYQKGLVIGAETVSKVLDWTDRGTAVLFGDG
                     AGGVLLEASKEKHFLAESLNTDGSRQGLQSSQVGLNSPFSDEVLDDKFLKMDGRAIFD
                     FAIKEVSKSINHLIETSHLEKEDIDYLFLHQANRRILDKMSRKIDIARDKFPENMMDY
                     GNTSAASIPILLSESYENGLLKLDGNQTILLSGFGGGLTWGSLIVKI"
     gene            2339..2563
                     /locus_tag="VBD27_001030"
     CDS             2339..2563
                     /locus_tag="VBD27_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7025052.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLNTTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKVK"
     gene            2718..3677
                     /gene="fabK"
                     /locus_tag="VBD27_001031"
     CDS             2718..3677
                     /gene="fabK"
                     /locus_tag="VBD27_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEO7025053.1"
                     /translation="MKTRITELLNIKYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSMTDKPFGVNIMLLSPFVDDIVDLVIEEGVKVVTTGAGNPGKY
                     MERFHEAGITVIPVVPSVALAKRMEKLGADAIITEGMEAGGHIGKLTTMTLVRQVVDA
                     VTIPVIAAGGIADGRGAAAGFMLGADAVQVGTRFVVAKESNAHPNYKAKILKAKDIDT
                     AVSAQVVGHPVRALKNKLVTTYSQAEKDYLAGRISINEIEELGAGALRNAVVDGDVIN
                     GSVMAGQIAGLIKSEETCQEILEDIYSGARQVILSEASRWSDL"
     gene            3697..4623
                     /gene="fabD"
                     /locus_tag="VBD27_001032"
     CDS             3697..4623
                     /gene="fabD"
                     /locus_tag="VBD27_001032"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO7025054.1"
                     /translation="MNKVAFLFAGQGAQKLGMARDLYETFPIVKETFDKASHVLGYDL
                     RELIDKDLDKLNQTKYTQPAILTTSTAIYRLILKEIGLRPDMVAGLSLGEYSALVASG
                     AIRFEDAVVLVSRRGQLMEAAAPAGSGKMVAVLNADRQIIEDACKKASQFGIVSPANY
                     NTPKQIVIGGESIAVNAAVEELKQQGVKRLIPLNVSGPFHTALLKPASQKLSDVLDKV
                     HFSVSEIPVIGNTEAQIMKKDDIKSLLARQVMEPVRFDESIETMKKMGMTQVVEIGPG
                     KVLSGFLKKIDSSLSVYSVEDKIGFNNLKELN"
     gene            4632..5366
                     /gene="fabG"
                     /locus_tag="VBD27_001033"
     CDS             4632..5366
                     /gene="fabG"
                     /locus_tag="VBD27_001033"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEO7025055.1"
                     /translation="MQLKDKNIFITGSSRGIGLAIAHQFAQLGANIVLNGRSEISEDL
                     IAEFADYGVKVIAISGDVSSFEDANRMIKEAIASLGSVDVLVNNAGITNDKLMLKMTV
                     EDFESVLKINLTGAFNMTQSVLKPMTKARQGAIINISSVVGLTGNVGQANYAASKAGL
                     IGFTKSVAREVAARGIRVNAIAPGFIESDMTDVIPEKMQEAILAQIPMKRIGKGKEVA
                     QVASFLAEQEYLTGQVIAIDGGMTMQ"
     gene            5382..6614
                     /gene="fabF"
                     /locus_tag="VBD27_001034"
     CDS             5382..6614
                     /gene="fabF"
                     /locus_tag="VBD27_001034"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO7025056.1"
                     /translation="MTLQRVVVTGYGVTSPIGNTPEEFWNSLKEGNVGIGPITKFDSS
                     DFMVKNAAEIHDFPFDKYFVKKDLNRFDMYSLYALYASSEAIQHANLNLDEIDADRFG
                     VIVASGIGGIQEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMRLGAHGVCKSI
                     NTACASSNDAIGDAFRNIKFGIQDIMVVGGAEAAITKFAIAGFQSLTALSTTEDPSRA
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     LGATKAIQLALAEANIKPEEVNYVNAHGTSTPANEKGESQAVVAALGTDVPVSSTKSF
                     TGHLLGAAGAVEAIATIEAIRHSYVPMTAGTTELSEDITANVIFGQGQDADIRYAISN
                     TFGFGGHNAVLAFKRWED"
     gene            6580..7116
                     /locus_tag="VBD27_001035"
     CDS             6580..7116
                     /locus_tag="VBD27_001035"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO7025057.1"
                     /translation="MQFWPSNAGRIKLDIQEIKDLMTQFDESSLREFSFKTSDGELSF
                     SKNEGKAPLVPTMSPMSHQPEATPTIATPVSNEAGEQTKQATEVVSEIPESTVTVAEG
                     DVVESPLVGVAYLASGPDKPNFVSVGDSVKKGQTLMIIEAMKVMNEVPAPHDGVVTEI
                     LVANEEVIEFGKGLVRIK"
     gene            7113..7535
                     /gene="fabZ"
                     /locus_tag="VBD27_001036"
     CDS             7113..7535
                     /gene="fabZ"
                     /locus_tag="VBD27_001036"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO7025058.1"
                     /translation="MIDIKEIREALPHRYPMLLVDRVLEVSEDEIVAIKNVSINEPFF
                     NGHFPEYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTAKFVKRRGTIAVVEAIAEVDGKLAASGTLTFAIGN"
     gene            7573..8943
                     /gene="accC"
                     /locus_tag="VBD27_001037"
     CDS             7573..8943
                     /gene="accC"
                     /locus_tag="VBD27_001037"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO7025059.1"
                     /translation="MFKKILIANRGEIAVRIIRAAREMGISTVAIYSEADKESLHTIL
                     ADEAICVGPAKSAESYLNVNAILSAAIVTGAEAVHPGFGFLSENSKFATMCEEMNLKF
                     IGPSGEVMDKMGDKINARAEMIKADVPVIPGSDGQVTSVEEAVSIAEEIGYPLMLKAS
                     AGGGGKGIRKVKSADELKPAFESASQEALAAFGNGAMYIEKVIYPARHIEVQILGDSF
                     GKIVHLGERDCSLQRNNQKVLEESPSVAIGNTLRQQIGEAAVRAAEAVSYENAGTIEF
                     LLDENSGQFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIRIAAGIPLSVSQNDIKLTGH
                     AIECRINAENPQFNFAPCPGTINGLHLPAGGMGLRVDSAVYTGYTIPPYYDSMIAKVI
                     VHGENRFDALMKMQRALYELEIDGIITNTEFQMDLISDKKVLAGDYDTSFLMEDFLPR
                     YYGNNQ"
     gene            8952..9827
                     /locus_tag="VBD27_001038"
     CDS             8952..9827
                     /locus_tag="VBD27_001038"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO7025060.1"
                     /translation="MALFSKKDKYIRISPNKALGSSDKRSLPEVPDELFAKCPSCKHM
                     IYQKDLGLAKICPACSYNFRISAQERLLLTVDEDSFEELFTGIETKDPLNFPNYREKL
                     AATRQKTNLDEAVVTGLAKIKGQTTALAIMDSHFIMASMGTVVGEKLTRLFELATEKK
                     LPIVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNQGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQALVGFAGRRVIETTVREDLPEGFQKAEFLLEHGFVDAIINRTELRDC
                     IAQLIAFHGNEVGHE"
     gene            9820..10593
                     /locus_tag="VBD27_001039"
     CDS             9820..10593
                     /locus_tag="VBD27_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEO7025061.1"
                     /translation="MNNDVTRILKDARDQGRLTALDYAELIFDNFMELHGDRQFADDK
                     SIIGGLGYLAGRPVTIVGIQKGKNLQDNLDRHFGQPHPEGYRKALRLMKQAEKFGRPV
                     ITFINTAGAYPGVGAEERGQGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     KVWMLEHTVYSILSPEGFASILWKDGTRTTEAAQLMKMTAGELYHMEVVDKVIPEHGY
                     FSSEIVDMIKTSLISELEMLSQLSLEDLLEQRYQRFRKY"
     gene            11061..11693
                     /locus_tag="VBD27_001040"
     CDS             11061..11693
                     /locus_tag="VBD27_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEO7025062.1"
                     /translation="MYQELNDYLEFQSMKYIMPEKAGPLKDDMQTFKAKGQLARNSFT
                     ELSRALEQRMDGFKMQRVSNWANQAQVGRPHFWVYYRKDTDQLDDVAVALRVYGVKDS
                     FGVSLEVSFVERQKSDKTLEKQARVLSIPIASPLYFMVQRQGETHREEGNEENRQRLM
                     QEIKSGKVRKVLVKYDVLLTENQSLENILQRLLEGFEKVLPYYQVCQTIS"
     gene            complement(11739..13016)
                     /gene="serS"
                     /locus_tag="VBD27_001041"
     CDS             complement(11739..13016)
                     /gene="serS"
                     /locus_tag="VBD27_001041"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO7025063.1"
                     /translation="MLDLKRIRTDFDAVAKKLATRGVDQETLTTLKELDIKRRELLIK
                     SEEAKAQRNVASAAIAQAKRNKENADEQITAMQTLSADIKAIDAELADVDANLQSMVT
                     VLPNTPADDVPLGADEDENVEVRRWGTPREFDFEAKAHWDLGESLGILDWERGAKVTG
                     SRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTF
                     ELADSPFVLIPTAEVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN"
BASE COUNT         4231 a         2030 c         2878 g         4071 t
ORIGIN      
        1 aatataatat atggtattga tggcagcgtg gccactatta tgcttaatcg accagatatt
       61 tcaaatggtt ttaacatccc aatgtgtcaa gaaattattg atgcaataag gcttatgtct
      121 gaaaataaag atgtcatgtt tttagtgatt gaagcacaag gaccaatctt ttcaatcgga
      181 ggagatttaa aagtgatgaa agatgctgtt gagagcgatg atattagctc attaactaaa
      241 attgctgagt tagttaatca gatatcgtat gatctactac agctagagaa acctgttgtt
      301 atgtgtgtag atggggctgt agctggtgcc gcagctaata ttgcattagc agctgatttt
      361 gttatcgcaa gcaagaagag taaatttatc caggcttttg tgggtgtcgg tttagctcca
      421 gatgcaggag gtttattgtt acttagtaaa tcaatcggta taactcgtgc agtgcaacta
      481 gctttaactg gagaaagctt atctgctgaa aaagctgaag ctttgggaat tgtttataaa
      541 ctttgtgaat cagataaaat aggtaaaatt aaggatcaac tattaaagcg tttaagtaga
      601 cattctataa actcatatca agccataaaa tcattagctt gggaagctgc ttttaaagat
      661 tgggaacaat ataaaaagct tgaattacag ttacaagaat cattagcctt taagcaagat
      721 tttaaagagg gtgttcgggc acacgctgat agaaggcgtc ctaatttttt aggcaaatag
      781 tttgaaaatc aaattattta agattaaagt cttgcaaata agtttgaact ttgatataat
      841 ttgactatca aagtttaatg tgaggtggtg atatcttgga atacgatcag attaatagtt
      901 atttagttga catttttaat agaatcatga ttattgagga aatgagttta aagactagcc
      961 aatttagcga tgtatcttta aaagaaatgc atactattga aattattggg aaacattctg
     1021 aggtaactcc tagcgatgtc gctcgagaat taatgttaac tttgggaact gtaacaacca
     1081 gccttaataa gttagaaaaa aaaggctaca ttgagcgtaa acggtcaagt attgatcgtc
     1141 gagttgttca cttgagtctt actaagagag gtaggttatt agatcgattg catagtaaat
     1201 ttcataaatc tatggtatct catattattg aagacctagg cgaagaagac ataaaaatgt
     1261 tgacgagcgc tttagggaat cttcataaat ttttggagga cttagtctag tggtttttgc
     1321 taaaattagt caacttgcac attatgcacc ttctcaaatc attaaaaatg aggatttatc
     1381 attaatcatg gataccagtg atgactggat ttcgtcacgt actggtataa aacaacgtca
     1441 tattagtaaa aatgaaacaa cagctgattt ggcaaataag gtcgcagaac aacttattga
     1501 aaaatccggt tattccgcta gtcaaattga ctttattatt gttgcaacga tgacaccaga
     1561 ctctatgatg ccttctactg ccgcaagagt gcaagctcat attggagcga gtaatgcttt
     1621 tgcctttgac ttgagtgcag catgtagtgg ttttgttttt gccttgtcta ccgccgaaaa
     1681 attgatatca tcaggtagct atcaaaaagg tttagtaata ggagcagaaa ctgtttctaa
     1741 agtattagac tggactgata gagggacagc agttttattt ggagatggag ctggaggtgt
     1801 cttgctagaa gcttctaagg aaaaacattt tcttgcagag tctctcaata cagatggtag
     1861 tcgtcaggga cttcaatcta gtcaagtcgg gttaaattca cctttctcag atgaagtact
     1921 tgatgataaa tttttgaaaa tggatggtag agcaatcttt gattttgcta tcaaagaagt
     1981 ttcaaaaagt atcaatcatc ttatagaaac atcccatttg gaaaaagaag atattgatta
     2041 tcttttctta caccaagcga atcgaaggat tttagataag atgtctcgaa aaattgatat
     2101 agcacgtgat aaatttccag agaatatgat ggattatggt aatactagtg cagctagtat
     2161 ccctatttta ctttcagaat cttatgagaa cggtttgttg aaactagatg gtaaccaaac
     2221 aatcctctta tcgggtttcg gtggaggttt gacatgggga agtctaattg ttaaaattta
     2281 gttatatata gtgcatagca catttataaa agattattta aaatttaagg agctaaaaat
     2341 ggcagtattt gaaaaagtac aagaaattat tgttgaagaa cttggtaaag atgcagaaga
     2401 agtaacgttg aacactactt ttgacgatct tgatgctgat tcgcttgatg ttttccaagt
     2461 gatttcagaa attgaagatg catttgatat ccaaattgag acagaagaag gactaaatac
     2521 tgttggtgat ttagttgctt atgttgaaga aaaagtaaaa taattaagtc gtaagaaaaa
     2581 aagaagcaag tgataataaa agatattctt accaaatcac ttattagagt gagtattcaa
     2641 tcaatcgttg atcggtactc actcttcttc ttatcaatat agtttgattg tcaaattatt
     2701 aattttagga aagaaaaatg aaaacacgta taacagaatt actaaatata aaatatccaa
     2761 tttttcaagg gggtatggcg tgggtcgctg atggagatct tgcaggagct gtttctaagg
     2821 caggtggtct aggtattatt ggtggaggaa atgcccctaa agaggttgtc aaagcaaata
     2881 ttgataaaat aaaatctatg actgataaac cctttggtgt gaatattatg cttttatcac
     2941 cttttgtaga tgatattgtt gatttggtta ttgaagaagg tgtaaaagtt gtaacgactg
     3001 gtgcaggtaa tcctggtaag tacatggaac gctttcatga agcaggtata acagtaatcc
     3061 ctgttgttcc tagtgttgca ttagcaaaac gtatggagaa attaggggct gatgctatca
     3121 ttacagaagg aatggaagcg ggtggtcaca tcgggaagct gaccacgatg acattagtgc
     3181 gacaggttgt tgatgctgtt acaattccag tcattgccgc aggtggtata gcagatgggc
     3241 gtggggctgc tgcgggtttc atgttaggag ctgatgctgt tcaggtagga actcgttttg
     3301 ttgttgctaa agaatctaat gctcacccta attacaaagc aaaaatttta aaagcaaaag
     3361 atatcgacac tgctgtttct gctcaggtag taggtcatcc agttcgtgcc ttaaaaaata
     3421 aactagtaac aacctatagc caagcagaaa aagattattt agctggccgt atttcaatca
     3481 atgagattga agaattagga gcgggagctc ttcgcaatgc agttgttgat ggggatgtta
     3541 ttaatggttc agttatggca ggacaaattg caggattaat taaatcagaa gagacttgtc
     3601 aagagattct tgaagatatt tattcaggag cgcgtcaagt tattctttct gaggcaagcc
     3661 gctggtcaga tttataaata caaataagga gacactatga ataaagttgc atttctattt
     3721 gctggtcaag gtgctcaaaa gttaggaatg gctagagatt tatatgaaac tttcccgatt
     3781 gttaaagaaa cttttgataa ggcaagtcat gttttaggat atgatttgag agaattgatt
     3841 gataaagact tagacaaatt aaatcagacg aaatatacgc aaccagctat tttaacgact
     3901 tcgactgcta tttatcgttt aattcttaaa gaaattggac ttagaccaga tatggttgca
     3961 ggactttctt tgggagaata tagtgcttta gttgcttcag gtgctataag atttgaagat
     4021 gctgtagtac ttgtttctag acgaggccaa ctaatggaag cagcagcacc tgctggtagt
     4081 ggcaaaatgg tagcggtttt aaatgctgat cgtcaaataa ttgaagatgc ttgtaagaag
     4141 gcttctcagt ttggtattgt tagtcctgca aattataata ctccaaagca aattgttata
     4201 gggggagagt caatcgctgt aaatgcagct gtagaagaac taaaacaaca aggagtaaaa
     4261 cgtttaattc ctctgaatgt ttcaggtcct tttcacactg ctttattgaa acctgcaagt
     4321 cagaaattga gcgatgtttt agataaagtt catttttcag tttctgaaat ccctgtaatt
     4381 ggaaatacag aggcgcagat aatgaaaaaa gacgatatca aatctttatt ggcacgccaa
     4441 gttatggaac ctgtacgctt tgatgagtct attgaaacca tgaaaaaaat gggaatgaca
     4501 caggttgttg aaattggtcc agggaaggta ctctcaggtt tcttgaaaaa aatagatagc
     4561 tcattatcag tatattctgt tgaagataaa attggattta ataatcttaa ggaattaaac
     4621 taggggaaaa tatgcaatta aaagataaga acatttttat tacgggttca agtagaggaa
     4681 tcggccttgc aattgcccat caatttgcac aattaggtgc taatattgtt ttaaatggac
     4741 gttctgaaat atcagaagac ttaatagctg aatttgctga ttatggtgta aaagttattg
     4801 ctatttcagg agatgtttct tcttttgaag atgctaatcg catgattaaa gaagctattg
     4861 caagtctagg tagcgtggat gtgcttgtta acaacgctgg tattactaat gataaattga
     4921 tgcttaaaat gactgttgaa gattttgaga gcgttcttaa aattaattta acaggtgcat
     4981 ttaatatgac acaatcagtc ttgaaaccga tgacaaaagc gcgtcaaggt gctattatta
     5041 atatctctag cgtcgttgga ttaactggaa atgtagggca agctaattac gcagcctcta
     5101 aagcaggttt aattggcttt acaaaatcag ttgccagaga agtagcagca cgaggcattc
     5161 gtgtcaatgc cattgctcca ggctttatcg aatctgatat gacagatgtt atcccagaaa
     5221 aaatgcaaga agctatttta gctcaaattc caatgaaacg tattggaaaa ggtaaagaag
     5281 tagcgcaggt agcgtctttc ttagcagaac aagaatacct tacaggacaa gttattgcca
     5341 ttgatggcgg tatgacgatg caataataaa gaggaaataa tatgacatta cagcgagtag
     5401 tagtaacagg ttacggtgta acctcaccta ttggaaacac accagaagaa ttttggaata
     5461 gccttaaaga aggaaatgtt ggtattggac ctatcactaa atttgattca agtgatttca
     5521 tggtcaaaaa tgctgctgaa attcatgatt ttccatttga taaatatttt gtcaaaaaag
     5581 atctgaacag atttgatatg tattcccttt atgccttata tgcttcaagt gaagctattc
     5641 aacatgctaa tttaaattta gatgaaattg atgcagatcg atttggtgtc atcgttgcat
     5701 caggtattgg agggattcaa gagattgaag aacaagtgat tcgtcttcat gaaaaagggc
     5761 caaaacgcgt aaaaccaatg acgcttccaa aagctctacc aaatatggca gctggaaatg
     5821 ttgcgatgag gttaggcgct catggtgtat gtaaatcaat taatacagct tgtgcttctt
     5881 ctaatgatgc tattggagat gctttccgta atataaaatt tggtattcaa gatattatgg
     5941 tagtcggtgg tgcagaagct gctattacaa aatttgccat tgctggattc caaagtctta
     6001 cagcattatc aacaactgaa gatccatcaa gagcatctat tccatttgat aaagatcgca
     6061 atggatttat catgggagaa ggttctggaa tgttggtctt agaaagttta gaacacgctg
     6121 aaaaacgagg agctactatt cttgcagaag ttgttggtta tggtaacact tgtgacgcat
     6181 atcatatgac ttcaccacat ccagaaggtt taggagccac taaagctatt caacttgcac
     6241 tggctgaagc taatattaaa cctgaagaag ttaactatgt aaatgctcat ggaacttcaa
     6301 cacctgctaa cgaaaaagga gagagtcaag ctgttgttgc tgcacttggg acagacgttc
     6361 cagtttcttc gactaaatct tttactggtc atcttttggg tgccgcagga gctgttgagg
     6421 caattgcaac tattgaagcg atacgtcata gttatgttcc aatgactgct ggaacaacag
     6481 aattatcaga agatattaca gcgaatgtta tttttggtca agggcaagac gctgatattc
     6541 gttatgccat ttcaaataca tttggttttg gaggacacaa tgcagttctg gccttcaaac
     6601 gctgggagga ttaaattgga tattcaagag attaaggatt taatgactca atttgatgag
     6661 tcaagtctga gagaattttc attcaaaaca agtgacggtg agctgtcttt tagtaaaaat
     6721 gagggaaaag ctccacttgt accaactatg tctcctatga gtcatcaacc tgaagcgact
     6781 ccaactatag caactcctgt atctaatgaa gcaggtgagc aaacgaaaca agctactgaa
     6841 gtagtttcag aaatcccaga gtcgactgtg actgttgcag aaggtgacgt tgtcgaaagt
     6901 cctttagttg gggttgctta tttagcttct ggaccagata aacctaactt tgtttcagta
     6961 ggtgatagcg tgaagaaggg acaaactttg atgattattg aggcaatgaa ggtgatgaat
     7021 gaagtgcctg cccctcatga tggtgtagtt actgaaattc ttgtagccaa tgaagaagtt
     7081 attgaatttg gtaaaggatt ggtacgaatc aaatgattga tataaaagaa attcgtgagg
     7141 ctttacctca ccgctatcca atgttattag tggatcgtgt tttagaggta tcagaagatg
     7201 agatagtcgc tatcaaaaat gttagtatca atgagccttt tttcaatggt cattttccag
     7261 aatatcctgt aatgccaggc gttttaatta tggaagcttt ggctcaaact gcaggtgttt
     7321 tagaattatc taaagaagaa aacaaaggaa agcttgtttt ttatgcaggt atggataaag
     7381 tgaaatttaa aaagcaagtt gttccaggag atcaattagt tatgactgct aagtttgtca
     7441 aacgtagagg aactatagcc gttgttgaag caatagcaga agttgatggg aaattagctg
     7501 ccagtggaac gttaacattt gcaataggta attgaagata gtattgaatt tagagggaga
     7561 gaagcatcaa aaatgtttaa gaaaatattg attgctaatc gcggagaaat tgcagtacgt
     7621 atcattcgtg cagcccgtga aatgggaatt tcgacagttg ctatctattc ggaggctgac
     7681 aaggaatcat tgcacacaat tttagcagat gaagctattt gtgttggacc agccaaatca
     7741 gcagaatctt atttaaacgt taatgctata ttatctgctg ctattgtgac tggagcggaa
     7801 gctgttcatc caggttttgg gtttttgagt gaaaattcta aatttgctac tatgtgtgaa
     7861 gagatgaact taaaattcat tggtccatct ggagaagtta tggataaaat gggtgataaa
     7921 atcaatgctc gtgcagagat gataaaagct gatgttccgg ttatcccagg atcagatggt
     7981 caagttacaa gtgtagaaga agctgtatca attgctgagg aaatcggcta tccattgatg
     8041 ttaaaagcct cagccggtgg cggtggaaaa ggaattagga aggtcaaatc agcagatgaa
     8101 ttgaagcctg cttttgagtc tgcttcacag gaggctttgg ctgcgttcgg taatggtgct
     8161 atgtatattg aaaaagttat atacccagct agacatattg aagtacaaat acttggagat
     8221 agctttggta agatagttca cctaggagaa cgtgattgtt ctctgcagcg taacaatcag
     8281 aaggtattgg aagaaagtcc gtctgtggca ataggtaaca ctctgcgtca acaaattggt
     8341 gaagcagctg ttcgtgcagc tgaggctgta tcttatgaaa atgcaggaac aattgagttt
     8401 ttattagatg agaatagcgg acagttttat ttcatggaaa tgaatactcg tgttcaagta
     8461 gaacaccctg ttacagaatt tgtaacgggt gttgacatcg ttaaagagca gattcgtatc
     8521 gctgctggaa ttcctttatc agtttctcaa aatgatatta agttaactgg tcatgcgatt
     8581 gaatgtcgta ttaacgcaga gaatccacaa tttaattttg cgccatgtcc aggtaccatt
     8641 aatgggttac atctacctgc tggcggtatg ggcttacgtg tcgattcagc ggtttacaca
     8701 ggctacacta ttcctcctta ttatgatagt atgattgcga aagtaattgt tcacggagaa
     8761 aatcgttttg atgcacttat gaaaatgcaa cgtgccttat atgagcttga aattgatggt
     8821 attataacaa atacagaatt tcaaatggat ctcatttctg ataaaaaagt cttggctgga
     8881 gattatgata cttcattcct gatggaagac tttttaccaa ggtattatgg taacaatcaa
     8941 taggagggaa gatggcttta tttagtaaaa aggataaata tatccgtatt agtccaaaca
     9001 aagcattagg gtcttctgat aaacgtagtc ttcctgaggt tcctgatgaa ctctttgcta
     9061 agtgtccttc ttgtaaacat atgatttatc agaaagattt aggattagca aagatttgtc
     9121 cagcttgttc ttacaatttt agaatttcag cgcaagaacg tctgttgcta acagttgacg
     9181 aagatagttt tgaggaatta tttacaggga ttgaaactaa ggatcctttg aattttccaa
     9241 actaccgtga aaaactggct gctacacgtc aaaagactaa cttagatgaa gcggtggtga
     9301 caggtttagc taaaataaaa ggacaaacta cggccttagc tattatggat tctcatttta
     9361 taatggcgtc tatgggaacg gttgtaggcg aaaagttgac aagattattt gagctagcaa
     9421 ctgaaaagaa attacctatt gttattttta cagcgtcagg gggcgccaga atgcaagaag
     9481 gtattatgag tttgatgcaa atggcaaaag tatcggcagc agttaaacgc cattctaacc
     9541 agggattgtt ttatttaaca attttaacag acccaacaac cggcggcgtt actgcgagtt
     9601 ttgctatgga aggtgatatt attctggctg agccgcaagc gttggttggt tttgctggtc
     9661 gtcgtgttat cgaaacaacg gtgagagaag atttacctga aggctttcaa aaggcagagt
     9721 ttttgttaga acatggtttt gttgatgcta ttataaatcg aacagaatta cgcgactgca
     9781 ttgctcaact aattgctttc catggaaatg aggttggtca tgaataatga tgtgacaaga
     9841 attttaaaag atgcgcgtga tcaagggagg ctaactgctc ttgactacgc tgaattgatt
     9901 tttgataatt ttatggaact tcatggtgat cgccagttcg cagatgacaa atcaattata
     9961 ggaggtttgg ggtacttggc agggcgtcct gtgacgattg tcggtattca aaaaggtaag
    10021 aaccttcaag ataatttaga ccgtcatttt ggtcaaccac atcctgaagg ttatcgtaag
    10081 gctttacgtt taatgaaaca agctgagaag tttggaagac ctgttataac cttcatcaat
    10141 actgctggag catatcctgg ggtaggtgct gaggagcgag gtcagggtga agcaattgct
    10201 agaaacctct tagaaatgag tgatttaaaa gtccctatta tagctattat tattggtgaa
    10261 gggggttctg gaggagcatt agctttagct gttgccgata aggtttggat gttggaacat
    10321 actgtttatt ctattttaag tcctgagggc tttgcctcta ttttatggaa agatggtaca
    10381 agaacaacag aagcagcaca attaatgaag atgactgctg gagaacttta ccatatggaa
    10441 gttgtagaca aagtaatccc tgaacatggt tatttttcaa gtgagattgt cgatatgatt
    10501 aagacaagtt taatttcaga gttagaaatg ctctcacaat tatctcttga ggatttattg
    10561 gagcaacgtt accaacgttt tagaaaatat taatagaaaa aggctgagtt atttactcag
    10621 cctatattta gtttttcaag gtattatcat agttatagtt tcatattttg ctataattga
    10681 taaaaaggag atatgtcaat gacactagca ttagccaatt tttagattgt agtctaatcc
    10741 aaaattttca gaaagagtca tttcgaacac tttattctaa gtaccattat ttaggctctc
    10801 cttaacttga gactctatct gttattgaag agaaattttc tagaaaaaat agctattgtt
    10861 agattttaga aggctatgaa aagattggtt tttaaagagt tctattaagt gatgaagaaa
    10921 gtaaaacttg gttagttata attgcgatta gactttgttt ctagcaacga gacttcgcta
    10981 tacatacttt aaaagaactt gaaagtatgt atagatatat aatcgtgaaa acttaactat
    11041 tgtttacatg gagaaaaaga atgtatcaag aattaaatga ttatttagaa ttccaatcaa
    11101 tgaagtatat tatgcctgag aaggcaggcc ctttaaagga cgatatgcaa acttttaagg
    11161 caaagggcca acttgctagg aatagcttta cagagttatc aagagcctta gaacaacgta
    11221 tggatggctt taaaatgcaa agagtgagta attgggctaa tcaagctcaa gtagggcgtc
    11281 cacatttttg ggtttattat cgaaaggata cagaccaatt agatgatgtg gctgttgcgc
    11341 taagggttta tggtgtaaaa gatagttttg gggttagtct tgaggtaagc tttgtagaga
    11401 ggcaaaaatc tgataagaca ctcgagaagc aggctagagt gttatcgatt ccgattgcct
    11461 ctccattgta ttttatggtc caaaggcaag gtgaaactca tcgagaggaa ggcaatgagg
    11521 aaaatcgtca acgtttaatg caagaaatta agtcagggaa ggtaaggaaa gtattggtaa
    11581 aatatgatgt tcttttgact gaaaaccaga gcttagaaaa tattctgcaa cggttgttag
    11641 aaggatttga gaaagtgcta ccttactacc aagtttgtca aactattagc taagttcatg
    11701 gaaaaaatga gttggtatac caactcattt tagagtgcct aatttggttt aataatatcg
    11761 atattaccca tatatggacg aagaacttct gggatagtca cagaaccatc ttcattttga
    11821 taattttcga ggattgctgc tacagtacgt ccaactgcaa gtcctgaacc atttaaagta
    11881 tgaagcaacc taactttgcc atcaacttcg tcacggtaac gaatttgagc acgacgtgct
    11941 tggaaatctt cagtattaga acatgaagaa atttctcgat aagtattttg agcgggaatc
    12001 catacctcta agtcatacgt tttagctgct gagaatccca tatctcctgt acacaaggta
    12061 atgacacggt atgggagatt aagtttttga agaatatttt cagcatttgc tgtcattttt
    12121 tctaattctt ggtacgattc ttcaggcttg gcaaatttca ccatttctac tttatggaat
    12181 tgatgaaggc gaattaatcc acgtgtgtca cgtcctgcag agcctgcttc tgaacggaat
    12241 gatggactca tggcagtaaa gtatataggt aattctttcc catcgataat ttcatcacga
    12301 taatagtttg ttaaaggaac ttcagctgtt gggataagta cgaatgggct atctgctaat
    12361 tcgaaagtat cttccttgaa ctttggatat tgtcccgtac cgaacattga atcgtggtta
    12421 accatataag gagggatcac ttcagtgtac ccttccttag cgtgttcatc cagcatgaag
    12481 ctgtagatag cacgctctaa acgagcacct agacctttgt agaagaggaa gcgagaacca
    12541 gtaacttttg caccacgttc ccaatcgaga attccaaggc tttcccctaa atcccagtga
    12601 gctttggcct caaaatcaaa ttcacgagga gttccccagc gacgaacttc aacattttca
    12661 tcctcgtctg ctccaagcgg cacatcatca gctggtgtat taggaagtac ggtcaccatt
    12721 gattgtaaat tagcatccac atctgccaat tccgcatcaa ttgctttgat atcagcggaa
    12781 agtgtttgca tagctgtgat ttgctcgtct gcattctctt tatttcgttt tgcttgagca
    12841 atggcagcag aagcaacatt acgttgcgct ttcgcctctt cagatttgat aagtaattca
    12901 cggcgtttta tatcaagttc tttaagggtt gttaatgttt cttggtcaac accacgcgtg
    12961 gccaattttt tagcgacagc atcaaaatct gtacgaatac gttttaagtc taacatatag
    13021 gaatctcctt tatactaaaa aaaacaccct gtcatgctgg agcgcaaagg cgcggttcca
    13081 tccagcttca cagaagtgca cttgattgat tgattaaatt atttcccata aatggaacac
    13141 ggtagaattt cataaaacaa tcttatctgc tcgcaacgac cgcagactct ctgaaaagaa
    13201 ctattatact
//
LOCUS       DATRQA010000050        13194 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.050, whole genome shotgun sequence.
ACCESSION   DATRQA010000050 DATRQA010000000
VERSION     DATRQA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13194
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.050"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            96..851
                     /locus_tag="VBD27_001042"
     CDS             96..851
                     /locus_tag="VBD27_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7025064.1"
                     /translation="MNRLENIISLVSQYQKIDVNTLSELLQVSKVTIRKDLDKLEGKG
                     LLHREHGYAVLNSGDDLNVRLSFNHKTKKEIAALAANMVSDNDTILIESGSTCALLAE
                     NICQTKRNVTILTNSCFIANYLREYDSCQIVLLGGEYQSSSQVTVGPLLKKMISLFHV
                     SLAFVGTDGFDPKLGFMGRDLMRSEVAQEMANAADEVIILTDSSKFNQTALVEQLPLS
                     TVSQVITDKHPNSDIANLFQEAEITIQSLLQVP"
     gene            970..1953
                     /locus_tag="VBD27_001043"
     CDS             970..1953
                     /locus_tag="VBD27_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEO7025065.1"
                     /translation="MKLERQKLLAKVAYLYYMEGKSQSEIANELGIYRTTISRMLAKA
                     REEGLVRIEISDFNPEIFQLESYFKSKYHLKDIEIVSSRKDSDTSEIEKDLAHVAAAM
                     IRKKIKENDKVGIAWGRTLSKVVEAMRPHPVSQVSFVPLAGGPSHINARYHVNTLVYE
                     MSRRFQGSCTFINATLVQENANLAKGILTSKYFEGLMDNWEKLDVAIVGVGGKPKSNE
                     QQWLDLLNQDDFQCLDEEAAVGEITCRFFNHSGDSVNQHLAKRTIGITLEQLQKVPNR
                     IAVAHGNYKAAALLAVLKKGYINHLVTDFSTALNILRLDKDTFVDTMYQKS"
     gene            2157..2480
                     /locus_tag="VBD27_001044"
     CDS             2157..2480
                     /locus_tag="VBD27_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO7025066.1"
                     /translation="MEIIVADQIIMGLILNAGDAKQHIYQALKLAKEGNFAESKIEIE
                     LADSALLEAHNLQTQFLAQEAGGTRTDISALFIHSQDHLMTSITEINLIKEIIDLRQE
                     LHIQK"
     gene            2497..2817
                     /locus_tag="VBD27_001045"
     CDS             2497..2817
                     /locus_tag="VBD27_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7025067.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKIAADKEGIEAHIEAYSQGKIAD
                     YAKDLDVALLGPQVSYTLDKSKSICDEYGVPIAVIPMADYGMLDGVKVLKLALSLLET
                     RKEA"
     gene            2819..4120
                     /locus_tag="VBD27_001046"
     CDS             2819..4120
                     /locus_tag="VBD27_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7025068.1"
                     /translation="MSKFDSQKIITPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     LGQLPFKGLNQAIANVFGPEWTEPFMQVYSGTFAIMGLISCFAIAYAYAKNSGVEPLP
                     AGVLSLSSFFILMKSSYIPVKGEAIADAISKVWFGGQGIIGAIIIGLVVGAIYTWFIQ
                     HHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYLIAKVTTGGTFIEMIYDIIQVP
                     LQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKSLLAANRLTL
                     DNGAHIVTQQFLDSFLILSGSGITFGLVIAMLFAAKSKQYKALGKVAAFPAIFNVNEP
                     IVFGFPIVMNPVMFLPFILVPVLAALIVYGAIAVGFMQPFSGVTLPWSTPAIISGFMV
                     GGWQGALVQIVILAISTAVYFPFFKIQDNITYKNECEMERG"
     gene            4301..6757
                     /locus_tag="VBD27_001047"
     CDS             4301..6757
                     /locus_tag="VBD27_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEO7025069.1"
                     /translation="MTKQLLTQKYTNDVQQNSQKHFGYLTERMYSYRDKVLDKKPFID
                     AERAILVTEAYQKHQEKPNVLKRAYMLQNILEKMTIYIDDETMIVGNQASSDKDAPIF
                     PEYTLEFVVNELDLFEKRDGDVFYITEETKEQIRNIAPFWENNNLRARAGVMLPEEVQ
                     VYMETGFFGMEGKMNSGDAHLAVNYQKLLEEGLIGFEKKARKAKADLDLTKPESIDKY
                     HFYDSILITIEAVKTYAERFAILAKKQAKTANTKRRQELLDIASICERVPYYPAETFA
                     EAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWI
                     KTITINKVRSQAHTFSSAGSPLYQNVTIGGQTRHKEDAVNPLSFLVLKSVAQTHLPQP
                     NLTVRYHANLDKSFMNEAIEVMKLGFGMPAFNNDEIIIPSFIKKGVSEEDAYDYSAIG
                     CVETAVPGKWGYRCTGMSYINFPKVLLITMNNGIDPASGKRFAPSYGHFTQMTSYKEL
                     KEAWDKTLRYLTRMSVIVENAIDISLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
                     ISGLQVGIANLSDSLAALKKLVFEEKRLTTLEVWQALQSDYAGPRGEEIRQMLINEAP
                     KYGNDDDYADSLVRECYDVYVEEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGT
                     LATPDGRHAGTPLAEGCSPSHNMDKKGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTL
                     AKEEDKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAF
                     FNVLSKATQDDIIARTEHAL"
     gene            6767..7435
                     /locus_tag="VBD27_001048"
     CDS             6767..7435
                     /locus_tag="VBD27_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO7025070.1"
                     /translation="MEFLLDTLNLEAIKKWHHILPLAGVTSNPTIAKKEGDIHFFQRI
                     RDVREIIGCEASLHVQVVAKDYQGILDDAAKIRQETDDDIYIKVPVTPDGLAAIKTLK
                     AEGYNITATAIYTSMQGLLAISAGADYLAPYFNRMENLDIDATQVIKELAQAIERTGS
                     SSKILAASFKNASQVTKALSQGAQSITAGPDIFESVFAMPSIAKAVNDFADDWKASQH
                     SEHI"
     gene            7503..8591
                     /locus_tag="VBD27_001049"
     CDS             7503..8591
                     /locus_tag="VBD27_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEO7025071.1"
                     /translation="MKVFASPSRYIQGKDALFQSIEHIKSLGQTPLILCDDVVYNIVG
                     ERFLSYLQDDLLPHRVSFNGEASDNEINRVVAVAKEKNSDLIIGLGGGKTIDSAKAIA
                     DKVNLPVIIAPTVASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVIAQAPK
                     RLLASGIADGLATWVEARAVLQKNGIAMAGGRQTLAGVAIAQACERTLFNDSLQALAA
                     CDVKVVTKALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALSGDIHHLTHGEKVAY
                     GTLTQLFLENRPKEEIDRYINLYQAIGMPTTLAELHLGDATYEELLKVGQQATIEGET
                     IHEMPFKISAEDVAAALLAVDRYVSNHQ"
     gene            complement(8743..9669)
                     /gene="cysK"
                     /locus_tag="VBD27_001050"
     CDS             complement(8743..9669)
                     /gene="cysK"
                     /locus_tag="VBD27_001050"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEO7025072.1"
                     /translation="MSKIYNSITDLIGNTPIIQLHHIVPEGAAEVYVKLESFNPGSSV
                     KDRIALAMIEDAEQKGILKAGDTIVEPTSGNTGIGLAWVGKAKGYNVIIVMPETMSIE
                     RRKIIQAYGAQLVLTPGSEGMKGAIAKAKEISAEQNAWLPLQFNNQANPEIHEKTTGR
                     EIIETFGEKGLDAFIAGVGTGGTITGVSRALKKVNPDVAIYAVEADESAILSGEQPGP
                     HKIQGISAGFIPETLATDSYDHIIRVTSNDAIETGRIIGGLEGFLAGISASAAIYAAI
                     EVAKQLGKGKKVLALLPDNGERYLSTSLYDFE"
     gene            complement(9760..10404)
                     /locus_tag="VBD27_001051"
     CDS             complement(9760..10404)
                     /locus_tag="VBD27_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEO7025073.1"
                     /translation="MNYKTIKSDGIVEEEIKKSRFICHLKRVESEEEGRNYITQIKKA
                     HYKANHSCSAMVIGEKGDIKRSSDDGEPSGTAGIPMLTVLEKQGLTNVVAVVTRYFGG
                     IKLGTGGLIRAYSGSVANAIKEIGVVEVKEQIGIRIQLTYPQYQTFDNFLKEHHLQEF
                     ETEFLEAVTCKIYVDPKEFEHTITNLTEFYQGKALLTEEGSQIVEIPFKAETDK"
     gene            10460..11749
                     /locus_tag="VBD27_001052"
     CDS             10460..11749
                     /locus_tag="VBD27_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7025074.1"
                     /translation="MENYLGRLWTKAQLSEQLRKIAINLPSFIKKGSDYICTRCSSSV
                     AKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTFSKTNSLKWKGGLTPYQNEVSEE
                     LLKGISSKENLLVHAVTGAGKTEMIYHSIAKVIDTGGSVCIASPRIDVCLELHKRLSN
                     DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHG
                     VKQALKKNGTSIFLTATSTTELERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQK
                     SLQTQKLPPKLYQLINKQRQTRYPLLLFFPHIAEGQVFTEMLRQAFPMEKIGFVSSKS
                     TSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVG
                     RALERPEGLLYFLHDGKSKSMHQAIKEIKNMNHIGGF"
     gene            11749..12415
                     /locus_tag="VBD27_001053"
                     /pseudo
     CDS             11749..12415
                     /locus_tag="VBD27_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
     gene            12492..13046
                     /gene="raiA"
                     /locus_tag="VBD27_001054"
     CDS             12492..13046
                     /gene="raiA"
                     /locus_tag="VBD27_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO7025075.1"
                     /translation="MIKYSIRGENIEVTEAIREYVETKLSKVEKYFNEEQELDTRVNL
                     KVYREKTAKVEVTILIDSITLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKYRE
                     KIPASQVFTTEFEAEPDEEAVSQRIVRTKNVNLKPMDVEEALLQMELLGHDFFIYTDA
                     EDNTTNVLYKREDGELGLIEAKGN"
BASE COUNT         4377 a         2171 c         2538 g         4108 t
ORIGIN      
        1 gtataccttt tatacgtaac aaagtcaatt acgaatgaaa gtttattgaa cttgtttaat
       61 aaataaaaaa tgataaaata gaaaggaggt gtttcatgaa tagattagaa aatattatta
      121 gtttagtatc ccagtaccaa aaaatagatg ttaatacact ctcggagttg ttgcaagttt
      181 ctaaagtaac catacgtaaa gatttagata aactagaagg caaagggctt ttgcatagag
      241 agcacggtta tgcagtttta aacagtgggg atgatcttaa tgttaggtta tcttttaacc
      301 ataaaactaa aaaagaaatt gctgccttag ctgcaaatat ggtgtctgac aatgacacta
      361 tcttaataga atcaggctct acttgtgctc ttttagcgga aaatatctgt cagacaaagc
      421 gaaatgtcac tattttgacc aactcttgct ttattgctaa ctatttacga gagtatgatt
      481 catgccaaat tgtcttactt ggtggtgagt atcaatcaag ttcacaggtt acggtaggtc
      541 ctctcttgaa aaagatgatt tcactttttc atgtctcgtt agcttttgtt gggactgatg
      601 gttttgatcc aaaactagga tttatgggta gagatttaat gcgttctgaa gttgcacaag
      661 agatggcgaa tgctgccgat gaagtcatta ttttaacaga ttctagtaag tttaatcaga
      721 ctgcattggt ggaacagctt ccgttgtcaa ctgtatcaca agttattaca gataaacatc
      781 caaattcgga tattgctaac ttgttccaag aagcagagat taccattcaa tctttattac
      841 aagtaccata aaagaggcta tttttcttac attttgcaca tttgtggcga taatagataa
      901 agtgtcatgt caaattgcac agatgtaacc tttttgttaa actgaagcaa atgctgaaag
      961 gttatgagta tgaaattaga gcgtcaaaaa ttgttagcta aagtagctta cctttactat
     1021 atggaaggga aaagccaatc tgagattgcc aatgaattag gaatttatcg tacaacaata
     1081 agtcgtatgt tagccaaagc gagagaagaa ggtcttgtta gaatcgaaat ttcagatttt
     1141 aatccagaaa tttttcagtt agaatcctat tttaaaagta agtatcacct taaagatatt
     1201 gagattgttt cttcgcgaaa agatagcgat acttcagaga tagagaagga tttagcacat
     1261 gtagctgcag ctatgattcg aaaaaaaatt aaagagaatg acaaggtggg gattgcttgg
     1321 gggaggacac ttagtaaagt agttgaagca atgcgtcctc atccggtttc tcaagtttct
     1381 tttgttcctt tggccggagg tccaagtcat attaatgcac gctatcacgt taatactctg
     1441 gtttacgaaa tgtcaaggcg tttccaagga agttgtactt tcattaatgc aactctagtt
     1501 caagaaaacg ctaatttagc gaaaggaata ttaacttcaa aatattttga ggggctaatg
     1561 gataattggg aaaaacttga tgtggcaatt gtgggcgttg gtggtaaacc taaaagcaat
     1621 gaacaacagt ggcttgatct tttaaatcaa gatgattttc aatgcttgga tgaagaagca
     1681 gctgtaggtg aaattacttg tcgttttttt aatcattcag gtgattctgt gaatcaacat
     1741 ttagctaaaa ggacaatagg aattacctta gaacaactac agaaagttcc taatagaata
     1801 gcggttgctc atggcaatta taaggctgcg gcactcttag ccgttttaaa aaaggggtat
     1861 attaatcatt tagttactga tttttccaca gccttaaata ttttaagatt agataaagac
     1921 acctttgtgg atactatgta tcaaaagagc taggatttat tcctagctct tttaataatg
     1981 tttctattac caagtgaatt gttatagaat aagcacaaaa gtgcgctatt atcataaagg
     2041 gcacaaggtc tattgacgac gattaaggga catgttacat ttgttttgcg aaagaaaatt
     2101 agaacctttt gtaagaaagt ttaaacttat aaaaaatcat taaagcggag gtgcttatgg
     2161 aaatcatagt cgcagaccaa attattatgg ggctcatttt gaatgctggg gatgctaaac
     2221 aacatatcta tcaagcctta aaacttgcaa aagaaggaaa ttttgcagaa agtaaaattg
     2281 aaatagaact agcagatagt gcactcctag aagctcataa tcttcaaaca caattccttg
     2341 cacaagaagc aggagggaca aggacagata tctcagcact ctttattcat tctcaggatc
     2401 atttgatgac aagtattact gagattaact tgattaaaga aattattgat ttacgccagg
     2461 aattacatat tcaaaaataa gtaaagggga tcaaatatga taaaaattgg tttattctgt
     2521 gcagcaggtt tttcaacagg tatgttagta aacaatatga aaatcgcagc ggataaagaa
     2581 gggatagagg cacatattga agcttattca caaggaaaaa ttgcagatta tgctaaagat
     2641 ttagatgttg cattacttgg tccacaagtt agctatactc tagataaatc aaaatcaatc
     2701 tgtgatgaat acggtgtacc gatagcagtt attccaatgg cagattatgg tatgttggat
     2761 ggtgtaaaag ttttgaaact tgctttgagt ttattggaaa caagaaaaga ggcttgatat
     2821 gtcaaagttt gatagtcaga aaataattac tccgattatg aagtttgtca atatgcgagg
     2881 gattattgca ctcaaagatg gcatgctagc aattttacca ctaacagttg ttgggagtct
     2941 ctttttaata ttagggcagc ttccatttaa aggtcttaat caagccatag ctaatgtttt
     3001 tggaccagaa tggacagaac catttatgca agtttattca ggaacttttg cgattatggg
     3061 cttgatttcc tgttttgcaa ttgcttatgc ttatgctaaa aatagtggtg tagaaccgtt
     3121 acctgctggc gttttatcac tttcgtcttt ctttatttta atgaaatcat cttatattcc
     3181 tgtaaaagga gaagcaatag cggacgctat ttctaaggtt tggtttggcg gtcaggggat
     3241 tattggagca attattattg gcttagtagt tggtgctatc tatacatggt ttatccaaca
     3301 tcatattgtt ataaaaatgc cagagcaagt accacaggca atagcaaaac aatttgaagc
     3361 tatgattcca gcttttgtta tctttctttt atcgatgatt gtttatttga ttgctaaggt
     3421 aacaactgga ggtaccttta ttgagatgat ttatgatatc attcaagtac cgttgcaagg
     3481 cttaacaggc tcactttatg gagcaattgg gattgctttc tttatttcat tcttatggtg
     3541 gtttggtgtc catggccaat ctgtggtgaa tggtattgtg actgctttgt tactatccaa
     3601 tttagatgcc aataagtctt tattagcagc aaatcgctta acattagata atggcgctca
     3661 cattgtaaca cagcagtttt tggatagttt cttgatttta tcgggctcag gaataacttt
     3721 tggactagtt attgcgatgc tttttgcagc aaaatcaaaa caatacaagg cacttgggaa
     3781 agtggccgct tttcccgcaa ttttcaatgt taacgagcca atcgtctttg gctttccaat
     3841 cgtaatgaat cctgtgatgt ttctgccttt tattttagta ccagttttgg cagctttaat
     3901 tgtttatgga gctattgcgg ttggttttat gcaaccattc tcaggtgtta ctttaccatg
     3961 gagtactcca gcaattattt ctggattcat ggtaggtggt tggcaaggtg cactcgtcca
     4021 aatagtcatt ttagctatct caactgctgt ttacttccca ttctttaaaa tccaagataa
     4081 tattacttac aaaaatgaat gtgaaatgga aaggggatag ctagcagata agaggttgag
     4141 gtgtgtaatg caccttaacc tttttctaaa aatgcacatt tgtgcaactt tagatgtagc
     4201 attctattct ttattttctt cttgttattt taggatttag gtgctattat atttacgaag
     4261 aaaaactttt tagtttcgaa agaaacaagc ggaggctgtt atgaccaaac aattactcac
     4321 tcaaaaatat acaaatgatg ttcaacaaaa ttctcaaaag cattttggct atttgacaga
     4381 gcgcatgtat tcttatcgcg ataaggttct tgataagaaa ccattcatag atgcagaacg
     4441 tgctatcttg gtaactgaag cctatcaaaa gcatcaggaa aaacctaatg ttttaaagag
     4501 agcttacatg ttgcaaaata ttttggaaaa aatgacaatc tatattgacg atgagacaat
     4561 gattgtcggt aaccaagctt catctgataa agatgctcct atttttccag aatacactct
     4621 agaatttgtt gtaaatgaac tagacctttt tgaaaaacga gatggtgacg tcttttacat
     4681 tacagaagaa accaaagaac aaattagaaa tattgcacct ttctgggaaa ataataattt
     4741 acgggctcgt gcgggcgtta tgcttccaga agaagttcaa gtttatatgg agacaggctt
     4801 ctttgggatg gaaggtaaga tgaattctgg ggatgctcac ttagctgtta actatcagaa
     4861 gttactagaa gaagggctta ttggctttga aaaaaaagca agaaaagcta aagctgactt
     4921 agatttaaca aagcctgaga gtattgacaa atatcatttc tatgattcta ttttaattac
     4981 aatagaagct gttaaaacct atgcagagcg ttttgctatc ctagcaaaaa aacaagctaa
     5041 aacagcaaat actaagagac gtcaggaatt gttagatatc gctagtattt gtgaacgagt
     5101 gccatattac ccagctgaaa cgtttgcaga agctgttcaa tcggtttggt ttattcagtg
     5161 tattcttcaa atagaatcaa acggtcattc tctatcttat ggacgctttg atcaatacat
     5221 gtatccatat gttaaagctg acttagaagc tggacgtgag actgaggatt cgatcgttga
     5281 gcgcctaact aacctatgga ttaaaacaat aactattaat aaggttcgta gccaagctca
     5341 tacgttttcg tctgctggta gtcctcttta ccaaaatgta acgattggag gacaaacccg
     5401 acataaggaa gatgctgtta atcctttatc atttctagta ctaaaatcag tcgctcagac
     5461 acacttacca cagcctaatc taacggttcg ttatcatgct aacctagaca agtcattcat
     5521 gaacgaagct attgaagtta tgaaattagg ttttggtatg ccagctttta ataatgatga
     5581 aattattatt ccatcattta ttaaaaaagg agtttccgaa gaagatgcct atgattattc
     5641 agcgattggt tgtgttgaaa cagcagttcc tgggaaatgg ggttatcgtt gtaccggcat
     5701 gagctatatc aacttcccta aagtgttatt aattacaatg aataatggca ttgacccggc
     5761 ttctgggaag agatttgcgc cttcttatgg gcatttcacc cagatgacct catataaaga
     5821 acttaaagaa gcttgggaca aaacacttcg ttacttaact cgaatgagtg ttattgttga
     5881 aaatgctatt gatattagtc tcgaaagaga agtacctgat attctttgtt cagctttaac
     5941 tgatgattgt attggacgag gtaagcattt aaaagaaggt ggtgcagtat atgactacat
     6001 atctggtcta caagttggaa tcgctaatct ttcagactca cttgctgcgc ttaaaaaatt
     6061 agtatttgaa gaaaaacgcc taaccacatt agaagtatgg caagcattgc agagtgatta
     6121 cgcaggtccg cgtggtgaag aaattcgtca aatgttgatc aatgaagcac caaaatatgg
     6181 aaatgatgat gactacgcgg atagtttagt acgtgagtgt tatgatgttt atgttgaaga
     6241 aattgctaaa tatcccaaca cacgctatgg acgtggacca atcggtggta ttcgctattc
     6301 aggaacgtcc tctatttcag caaatgtagg tcaaggacga ggtactttag caactccaga
     6361 tggtagacat gcaggaactc cattagctga aggttgttca ccatctcata atatggacaa
     6421 aaagggacct acctctgttt tgaaatcagt ttcgaagtta ccaactgatg agattgtcgg
     6481 cggtgtactc cttaaccaaa aagtcaaccc tcaaaccttg gcaaaagaag aagataaaca
     6541 aaaattaatt gccttactta gaacattctt caatcgcctt catggttacc atatccagta
     6601 taatgttgtc tcacgtgaga ctttgattga tgctcagaag caccctgaaa aacaccgcga
     6661 tttgattgtg cgtgtagcag gttattctgc attctttaat gtcctatcta aggcaactca
     6721 agatgatatt attgcacgta cagaacatgc tctttaggag attattatgg aatttttact
     6781 tgacacttta aatttagaag ctataaaaaa atggcatcat atactgccac tagcaggtgt
     6841 aacttcaaat cccaccattg ctaagaaaga aggagatata cattttttcc aacgcattcg
     6901 tgatgttcga gaaattattg gatgtgaagc aagtttgcat gttcaagtag tggccaaaga
     6961 ttatcaaggg atactggatg atgctgctaa aatacgccaa gaaacggatg atgacattta
     7021 tatcaaggtt ccagttacgc cagatggatt agcagcaatt aaaacgttaa aagcagaagg
     7081 ttataatatt acagccacag caatatacac cagtatgcaa ggtcttttgg ctatatcagc
     7141 tggagcagac tacctagcac cttactttaa ccgaatggag aaccttgata ttgatgcgac
     7201 acaagttatt aaggaattag cgcaagcaat agagaggaca ggttcttcta gcaaaatttt
     7261 agcagctagt tttaaaaacg cttcacaagt aacgaaggcc ttatcacagg gtgctcaatc
     7321 aatcaccgcg ggaccagata tttttgaatc agtctttgcg atgccatcta ttgcaaaagc
     7381 tgttaacgac tttgcagatg attggaaagc cagtcaacac tcagaacata tttaacagag
     7441 aacgccaact tttgaggctg tatctttaac ataaaatttt ttaaagaaga aagaggttga
     7501 gaatgaaagt tttcgcaagt ccatcgcgct atatacaagg aaaggatgct ttattccaaa
     7561 gtattgaaca tatcaaatct ttaggtcaga ctccacttat cctttgtgat gatgttgttt
     7621 ataatatcgt tggagaaagg tttttatctt atctacaaga cgatttgtta ccacatcgtg
     7681 tctcctttaa cggagaagca tctgataatg aaatcaatcg tgtcgttgct gttgcaaaag
     7741 aaaagaatag tgaccttatt attggacttg ggggtggaaa aactattgac tctgcaaagg
     7801 caattgcaga taaagttaat ttaccagtga tcattgcacc aacagtagct tcaacagatg
     7861 ccccaacatc agcgttatca gtaatctata cagatgaagg agcttttgaa aaatatattt
     7921 tttattcaaa gaatccagac ttagttttgg tcgatactca agtcattgct caagcgccaa
     7981 aacgtttact agcttctggg attgcagatg gcttagctac ttgggttgaa gcgagagcag
     8041 tcttacaaaa aaatgggata gcgatggctg gcggtagaca gactttagcg ggcgtggcaa
     8101 ttgctcaagc atgtgagcgt actttattta atgatagttt acaagcttta gcggcttgtg
     8161 atgtcaaagt ggttacaaaa gctttagaaa atgttattga agctaatacc ttacttagtg
     8221 gtttaggttt tgaaagcgca ggattagctg cagctcatgc tattcataat ggttttactg
     8281 ccttgagtgg agatattcat cacttaaccc atggtgaaaa agttgcctat ggaacgttga
     8341 cacaactttt cctcgaaaat agaccaaagg aagaaattga tcgctacatt aatctttacc
     8401 aagcgattgg aatgccaact acacttgcag aactccattt gggtgatgca acatatgaag
     8461 aacttttaaa agttggtcag caagcaacaa ttgaaggtga aacaatacat gaaatgccat
     8521 tcaaaattag tgcagaggat gtcgctgcag ccctgttagc agttgaccgc tatgtttcaa
     8581 atcatcaata aaaacaatag aaccaagtga aattgcttgg ttctatttgt cgttttaata
     8641 ttttaactaa atagggctta ctagtattta aagagatata aactaaaaaa gatcagacaa
     8701 catatctaat cttttttagt tatcgtttac tgaagattaa agttattcaa aatcatacag
     8761 gcttgttgat aagtaacgtt cgccattatc agggagaaga gctaaaacct ttttcccttt
     8821 tcctagttgt ttggccactt caatagcagc ataaatagca gctgaagcag aaattcctgc
     8881 gaggaaacct tctagtccgc cgatgatacg tcctgtttca atagcatcgt tagatgtaac
     8941 gcgaatgata tgatcataag aatctgtagc caatgtttct ggaatgaaac ctgcagaaat
     9001 cccttgtatt ttatgaggcc caggttgctc accagaaagg attgcagatt catcagcttc
     9061 tacagcataa atagcgacat caggattgac tttttttaat gcacgagaga caccagtaat
     9121 tgtcccacca gttcccacac ctgcgataaa agcatctaaa cctttttcac caaaagtctc
     9181 gataatttcc ctaccagtcg ttttttcgtg gatttctgga ttggcttgat tattaaattg
     9241 caggggtagc caagcatttt gctctgcaga aatttctttt gccttagcaa tagctccctt
     9301 catcccttca ctaccaggtg ttagtactaa ttgagcacca taggcttgaa tgattttacg
     9361 acgttcaatt gacatggttt ctggcataac aataataaca ttataccctt tagcttttcc
     9421 tacccaagcc aaaccgatac ctgtattacc agatgtaggt tctacgattg tatctccggc
     9481 tttaagaatc cctttttgct ctgcatcttc aatcatagct agagcgatac gatctttgac
     9541 agatgaacct gggttaaagg attctaactt aacgtacacc tctgcagctc cttctggaac
     9601 aatatgatgt aattgaataa tgggtgtatt tccgattaag tctgtaatag aattataaat
     9661 ttttgacata agctatacct cttcatgaca ttggttaaga atattataaa actcacattg
     9721 attaaagtaa aatatcaatt ctttatcaag tgatagctat tatttatcgg tttcagcctt
     9781 aaagggaatt tcaacaattt gactcccttc ttcagttaac aaagcttttc cttggtagaa
     9841 ctctgtcaaa ttagttattg tgtgttcaaa ttccttaggg tcaacgtata ttttacaggt
     9901 tacagcttct aaaaattctg tttcaaactc ctgaaggtgg tgctctttta ggaagttgtc
     9961 aaaagtttga tattgaggat aagttaattg aatacgaata ccaatttgtt ctttaacttc
    10021 tactaccccg atctctttga tggcattagc tacgctacca gagtaggcac gaataagccc
    10081 ccctgtgcct aatttgatac ctccaaaata ccgtgtaacg acggcaacaa catttgtcag
    10141 gccttgcttt tcaagtacgg ttagcatagg gatacctgct gtaccactag gttcaccatc
    10201 atcactagat cgttttatat cccctttttc gccaataacc atagctgaac acgaatgatt
    10261 ggctttgtag tgcgctttct tgatttgcgt aatataatta cgcccctcct cttcggactc
    10321 tactcgcttt agatgacaaa taaatcgcga ttttttaatt tcttcttcga caattccgtc
    10381 tgattttatc gttttatagt tcatagagaa attttacaaa aattcttcaa atttagcaaa
    10441 aaaatccgaa tgaaataata tggaaaatta tttaggtaga ctttggacaa aagcacaatt
    10501 atctgagcaa cttagaaaaa ttgcaattaa cttgccatct tttataaaga aaggaagcga
    10561 ctatatttgt actcgatgct ctagttctgt tgccaaaaat tgccaattac caactggaaa
    10621 ttattattgt agagaatgta tagtttttgg aagagttact agtaatgaaa atttatatta
    10681 tttcccacaa aaaacatttt ctaaaacgaa ttctttaaaa tggaaagggg gactgacacc
    10741 ttatcaaaat gaggtttcag aagagttgtt gaaaggtatt tcaagtaagg aaaatcttct
    10801 tgttcatgct gtgacagggg ctggtaaaac agaaatgata taccactcta tcgccaaagt
    10861 aattgatact ggagggagcg tttgtattgc cagtcctaga attgatgttt gtttagaatt
    10921 gcataagcga ctttcaaacg attttagatg tgccataaca ttaatgcatg gagaatctcc
    10981 ttcttatcag agatcccctt taactattgc cacaacacat caattgttaa aattttatca
    11041 tgcttttgat ttgctaattg ttgatgaagt tgatgctttt ccatatgtcg acaacccgat
    11101 actataccat ggtgtaaaac aggccttaaa gaaaaacgga acaagtattt ttctgactgc
    11161 aacttcaacg actgagttag aaagaaaagt tgcaagaaaa gaactaaaaa aattacatct
    11221 agctaggaga tttcatgcca atccgttagt gatacccgag atggtttggg taagtggtat
    11281 ccagaaaagc ttgcagacac aaaaactacc tcccaaatta tatcagctga taaataaaca
    11341 gcgacaaacg agatatcctt tgttattatt tttcccccat attgcagaag gtcaggtatt
    11401 caccgaaatg ttaagacaag cctttcctat ggaaaaaatt ggttttgttt caagtaagag
    11461 cacatctagg ttaaagttag tgcaagattt tcgagataac aaattgtcta tcctagtatc
    11521 aactaccatt ctagagagag gagtaacttt ccctagcgtc gacgtttttg ttatacaagc
    11581 aaatcatcat ttgtttacga aaagttcctt ggtacaaatt tcaggaagag ttggaagagc
    11641 attagaaaga cccgaagggc tcctttactt ccttcatgat gggaaaagta aatcgatgca
    11701 ccaagccata aaggagatta aaaacatgaa tcatatagga ggattttaat gacttgtttg
    11761 ttatgtcatg aaatagattt atcgcaatta acttttgtgg aacttatact tctaaaacca
    11821 aagcaaaacg taatttgtca aacgtgcaag ggtagttttg aagcacttag tagggagatg
    11881 ggttgccaaa catgttgcaa gcaaatcctc cgaaaacaat gtcaagattg tatttattgg
    11941 ggtaaaaaag gtatagaggt aaatcatttt tccctttata gatacaatga agcaatgaaa
    12001 aaaatatttt tctcttttta aatttcaagg ggattatttg ttgaaagatg tttttacaaa
    12061 agaaataaag gctgctctaa aaaggtataa gggctacact atagtgccag ttcccttgag
    12121 tcatgaagga taccaaaata ggcaatttaa tcaagtgatt gcttttctac aatcggcaaa
    12181 tataccttat aaaaatattc tttctaaaaa agatggaggt aagcaatcgg ctaataataa
    12241 agaagaaaga ctcaaacaag ttcagcaatt tacattaaaa aatgaggctg agttaaggga
    12301 taacctttta attgttgatg atatttatac aacaggcgca acaatagcac aaatcaggaa
    12361 actattagaa gaaaaaggta taaaaaatat aaaaagtttt tcattagcac gctaaaatct
    12421 tgcaaatttt catgaaagcg gttataataa gaatatagaa agcgttaaca acgcaagaaa
    12481 gaggtactca tatgattaaa tacagtattc gtggtgaaaa catcgaagta acagaagcta
    12541 ttcgtgagta tgttgagacc aaactctcta aagttgaaaa atactttaat gaagaacaag
    12601 aattagatac tagagtcaac ctcaaagtat acagagagaa gaccgcaaaa gttgaagtaa
    12661 caatcttaat agattctatt actttacgag cagaagatgt ttcacaagat atgtatgggt
    12721 caattgactt agttgttgat aaaattgagc gtcaaattcg taaaaataaa actaaaattg
    12781 cgaaaaaata tcgtgaaaaa attccagcaa gtcaagtttt cacaacagaa tttgaggctg
    12841 agccagatga agaagcagta agccaaagaa ttgtacgtac taagaatgtt aatctaaaac
    12901 caatggatgt tgaggaagca ttgttgcaaa tggaactttt aggtcatgat ttctttatct
    12961 atacagatgc agaagataat acgacaaatg tcctttacaa gcgtgaagat ggcgaactag
    13021 gtttaattga agctaaagga aattaacttt gtaacataat ttaaaaaatc tcagagtttt
    13081 ttctgagatt tttttaaaac ctattgacaa caaataaaaa attatagtat actaaatgag
    13141 ttcttaagcg aacctaatat tgtattatca ttttataaac aacttaaaaa aagt
//
LOCUS       DATRQA010000051        12756 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.051, whole genome shotgun sequence.
ACCESSION   DATRQA010000051 DATRQA010000000
VERSION     DATRQA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12756
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.051"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            103..1122
                     /locus_tag="VBD27_001055"
     CDS             103..1122
                     /locus_tag="VBD27_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025076.1"
                     /translation="MKINKLLSDRLEACYSVCRLQNQDYDFLLVASEVDQACFAYDLN
                     SDLAKIVVWEDVGGTMSIIPIPGTMDFLATQKFYPGFKAKDCQVVYASFQGDHWDICN
                     AFDFPYLHRFDLIKGKENQLYFIGCTIANSKAYTEDWSDKGRIFVARFNTQNHTLEGL
                     QQLPQTLLKNHGYYAIQDEGYSLITSVEGVLKLTYPEFSTTGDWQLERLFDEETSDVV
                     KVDINRDGKDEYVIIQGFHGDRLRIFTEDFGRELFHYPEKTPFGHAIWSGRLLNQTCF
                     VFGWRSEKAELRLFHFVDGHLVSELVDAKAASSNVLAFEKDGKAYLFSANNGRGEVAL
                     YQLVK"
     gene            1141..2097
                     /locus_tag="VBD27_001056"
     CDS             1141..2097
                     /locus_tag="VBD27_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO7025077.1"
                     /translation="MDKKKILVTGIVPKEGLRKLMDRFDVTYSEDRPFSRDYVLEHLS
                     EYDGWLLMGQKGDKEMIDAGENLQIISLNAVGFDHVDTAYAKEKGIVVSNSPQAVRVP
                     TAEMTFALILAASKRLAFYDSIVRSGEWIDPSEQRYQGLTLQGSTLGIYGMGRIGLTV
                     ANFAKAFGMTVVYNDVYRLPEDKEKELGVTYLEFDQLIETADVITIHAPALPSTIHKF
                     NKDVFAKMKNRSYLINAARGPIVSEEALIEALKEGEIAGAGLDVFENEPQVSEGLRSL
                     DNVIMSPHAGTGTIEGRRTLAEEAADNIIAFFDGKPQNIVNK"
     gene            2195..3646
                     /locus_tag="VBD27_001057"
     CDS             2195..3646
                     /locus_tag="VBD27_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO7025078.1"
                     /translation="MLEALHKFFEVFGAATVVPAMIFIVSLFLKVNPRRAFFSALRAG
                     VGLTGFGWIISAFAPMVTKYIQQMVKTTGLHLPIVDIGWQAGSLTAFSSEIGLSFFVF
                     GLLIELGLFLLGITRVFVPSNLWNNFGYMIWGTMAYAATGNFILSFAFMVFVLLYSLV
                     MSEVLADRWSEYYGVKNATINSIHNIETLIPALILDPLWNLLGVNKVKLNPESLKTKL
                     GIFGEPMTLGFILGVIIGVLGSLRNLASIDTWGGILGFAVALAAVMTIFPLITGVFAS
                     AFAPLAEAVERNKKKESQAEQGALDKKRWFIAVDDGVGFGEPATIIAGLILVPIMVVI
                     SLILPGNEALPVVDLIAIPFMIEAMIAVSKGNILKAILNGIIWFSLGLYAASALGPIY
                     TEAVKHFGTALPAGVTLIMSFNLLAHPLTALVFFAWISGNPLYIGVTVIIYLAFLFLL
                     RTRRESIYAYLKTMADKNSGYEGSCEKIFIAED"
     gene            3668..4657
                     /locus_tag="VBD27_001058"
     CDS             3668..4657
                     /locus_tag="VBD27_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO7025079.1"
                     /translation="MKKIDGHAHIVDTIAGFNGKGRLNALGNGYAIWDDGDLIKLIPD
                     EYGDKAFTAESFLKYMDANGVDKAVILQGHLNGYQNYYTHLAIKRYPERFTGAFSVDP
                     FADNAMQIVKRHVEVLGFRAIKFEISQGGGIHGYRGQKTPFRLDTDPHVSRILTYLLD
                     YPGFVVTVDYGNWDQISHQPDAIANLARLYTSLDFVVCHLSFPHVEHSNRLRAELNMW
                     KNFNNIYTDISAIQDIDAPDSFPFPKSEQNVRIAKEVLGAKRIIWGTDSPWSATFNTY
                     EELATWLENVDIFSQEELEDVMYNNAERVYFKEEHVKANRAGIDDTTKELNLY"
     gene            4723..6240
                     /locus_tag="VBD27_001059"
     CDS             4723..6240
                     /locus_tag="VBD27_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEO7025080.1"
                     /translation="MTYYLSIDYGGTNTKALIFDKLGHQIAVSSFETLKNEAQSGHRQ
                     VNLVKTWNAITSAIREVIQISKLSPEQISAVACIGHGKGLYLLDNKLEPLEQGILSTD
                     NRAKDLAQYFESKLDNIWELTRQHIFPSQSPVILRWLKDYQPETYKSIGAVLSAKDFI
                     RYKLTGKVQQEYGDASGNHWINFQTGTYDPAILDFFGIREIENSLPELIDSADLVPGG
                     ISSQAAKETGLVEGTPVVGGLFDIDACALGSGVLESDTFSVISGTWNINTYPSLKPAK
                     QDSGLMTSYFPDRRYLLEASSPTSAGNLDFMLKMLMHQEIDNAKSSGGSIYDNLEEFL
                     THTDATHHGLIFFPFLYGSNTSQDASACFFGLTTKSTKSQMIRAVYEGIAFAHKQHIT
                     DLIKSRGGVPKIIRFSGGATNSPAWMQMFSDILNLPIKTVEGTELGGLGGAILARHAL
                     DKISLKEAVQDMVRVKAIYKPQLSEVKGYEKKYHAYQKLLETLDPIWSELGHLNK"
     gene            6260..7021
                     /locus_tag="VBD27_001060"
     CDS             6260..7021
                     /locus_tag="VBD27_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HEO7025081.1"
                     /translation="MKQEDIIINSIVFKKQLDKGFTQVQLIKLVKDLGFSRFEIRQEL
                     LQDPDRELPALKAEADFYDINLYYSANEDLIKGGKVNPYLNKGLKEASQLGAPFIKLN
                     VGQTRNLSKEELEPLKEILKSQTIGIKVENNQDPKAATVENCQYFMTLVKELQIPISF
                     VFDTANWAFINQDLYQAVNNLACDTTYLHCKNFIQVAGKPHLSKSLFEGEINLTDLLK
                     SFSNCEYLALEYPTELEILKRDVQRLISISNSQNF"
     gene            7087..8766
                     /locus_tag="VBD27_001061"
     CDS             7087..8766
                     /locus_tag="VBD27_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEO7025082.1"
                     /translation="MIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYYHLEK
                     INDALPSDVPQIVSYPRVGILLTEKQKAACRLLLDEVTDYSYVMKSSERLQLSLVSIV
                     VAKDRITIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFLDGHPLS
                     IIQYLYKLLDDIYHNGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLG
                     KKINSQDGQFMIQILPFILMAYRKMRLSPEVQTSLNSDFSLVWQRKEYEIAKELADEL
                     EENFQLSLDEIEVGLVAMLMLSFRKDRDNHLESQDYDDMRATLTSFLKELEERYHLHF
                     VHKKDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYEELFAITSSSVKLLEKAWQ
                     IKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDI
                     EAVFTTEQYQSVSDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNC
                     ANSNQFTNELEKTIAQYVKEDSERYVLKSKIEKLIHQELLQDVLPLQSTVC"
     gene            9083..10174
                     /gene="ulaG"
                     /locus_tag="VBD27_001062"
     CDS             9083..10174
                     /gene="ulaG"
                     /locus_tag="VBD27_001062"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEO7025083.1"
                     /translation="MPNVKDITRESWILSTFPEWGTWLNEEIEEEVVAEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVI
                     DPFAINELDYYLVSHFHSDHIDINTAAAIINNPNLDHVKFVGPYECGEIWKKWGVPEE
                     RIIVIKPGESFEFKDIKVTAVESFDRTCLVTLPVDGAEEHDGELAGLAVTDEEMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDYKIDVAINNYGDNPVGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DKDRIEYHHPRGFDDCFEQESNIQFKALL"
     gene            10264..12642
                     /locus_tag="VBD27_001063"
     CDS             10264..12642
                     /locus_tag="VBD27_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEO7025084.1"
                     /translation="MSEFDTKSYLEKLDAWWRAANYISAAQMYLKDNPLLRRELVEND
                     LKVHPIGHWGTVPGQNFIYAHLNRAINKYDLDMFYIEGPGHGGQVMVSNSYLDGSYTE
                     LNPNIEQTEDGFKQLCKIFSFPGGIASHAAPETPGSIHEGGELGYALSHATGAILDNP
                     DVIAATVIGDGEGETGPLMAGWLSNTFINPVNDGAVLPIFYLNGGKIHNPTIFERKTD
                     EELSQFFEGLGWKPIFADVVELSEDHAAAHALFAEKLDQAIQEIKTIQSEARQKPAEE
                     AIQAKFPVLVARIPKGWTGPKAWEGTPIEGGFRAHQVPIPVDAHHMEHVDSLLSWLQS
                     YRPEELFDENGKIVDEIAAISPKGDRRMSMNPITNAGIVKAMDTADWKKFALDINVPG
                     QIMAQDMIEFGKYAADLVDANPDNFRIFGPDETKSNRLQEVFTRTSRQWLGRRKPDYD
                     EALSPAGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYESFLRVVDSMVTQHFKWLRK
                     SKTHTTWRKNYPALNLIAASTVFQQDHNGYTHQDPGILTHLAEKTPEYIREYLPADTN
                     SLLAVMDKAFKAEDKINLIVTSKHPRPQFYSIAEAEELVAEGYKVIDWASNVSLNQEP
                     DVVFAAAGTEPNLEALAAISILHKAFPELKIRFVNVLDILKLRHPSQDARGLSDEEFN
                     KVFTTDKPVIFAFHGYEDMIRDIFFSRHNHNLHTHGYRENGDITTPFDMRVMSELDRF
                     HLAQDAALASLGNKAQAFSDEMNQMVAYHKDYIREHGDDIPEVQNWKWENIK"
BASE COUNT         3993 a         2142 c         2563 g         4058 t
ORIGIN      
        1 ttaacgaacc atctctaact ctcttaactc aaatgttttt atttttttgc accattagtt
       61 aaacggttaa ctcaagtttt atcttcacca aaggaggata gaatgaagat taataaatta
      121 ttgtcagaca gacttgaagc ttgttattca gtttgtcgtt tgcaaaatca agactatgat
      181 tttttattag tagcttcaga ggttgatcaa gcctgctttg cttatgattt gaatagcgat
      241 ttagcaaaaa ttgttgtttg ggaagatgtg ggaggtacca tgagtattat tccaattccc
      301 ggaaccatgg attttttggc aacccagaaa ttttacccag ggtttaaggc caaagattgt
      361 caggttgttt atgcaagttt tcaaggagat cattgggaca tttgtaacgc atttgatttt
      421 ccgtatttac atcgctttga tctcattaaa ggtaaagaaa atcaacttta ctttataggt
      481 tgtacaattg ctaacagtaa agcctacact gaggattgga gtgataaagg ccgaattttt
      541 gttgctcgtt ttaatactca aaaccataca ttggaaggat tgcaacaatt gcctcaaact
      601 ttattaaaaa atcatggata ctatgccatt caggatgaag gatattcatt gattacttca
      661 gtagaagggg tactcaaact cacttatcca gaattttcta ctacaggcga ctggcaatta
      721 gaacggcttt tcgatgagga gacaagcgat gtggtgaaag tggatattaa tcgggatggt
      781 aaggatgagt atgtgatcat ccaaggtttt catggagatc gtttacgtat cttcactgaa
      841 gatttcggtc gagaattatt ccattatcct gaaaaaaccc catttggtca cgctatttgg
      901 agtggtcgtt tacttaatca gacttgtttc gtattcgggt ggcgatcaga aaaagcagaa
      961 ttaaggcttt ttcactttgt agatgggcac ttggtttcag aattagtaga tgcaaaagca
     1021 gcttctagta atgtcttagc ttttgaaaaa gatggaaaag cttatctttt ctcagccaat
     1081 aacggacgtg gcgaagttgc tctttatcaa ttagtaaaat aaatttaaga aggagacaat
     1141 atggacaaga aaaaaatctt agtaacgggt attgtgccta aagaaggtct aagaaagctt
     1201 atggaccgat ttgatgttac ttattcagaa gatcgcccat tttcacgtga ctatgtgtta
     1261 gagcatttat ctgaatatga cggatggtta ctcatgggac aaaaaggtga taaagagatg
     1321 attgatgcag gtgaaaactt acaaattatt tctttaaatg cggttggttt tgatcatgta
     1381 gatactgcgt atgctaaaga aaaaggaatt gtagtctcta attcccctca agcagtacga
     1441 gttccgacag cggaaatgac ttttgcttta attttagctg ccagtaagcg ccttgcattt
     1501 tatgatagta ttgtaagaag tggagagtgg attgatccga gcgaacaacg ttatcaaggt
     1561 ttgactttgc aaggttcaac attaggtatt tacggaatgg gacgcatcgg tttgacagtc
     1621 gcaaattttg ctaaagcatt tgggatgact gttgtttaca atgatgttta tcgacttcct
     1681 gaagataagg aaaaagagct tggtgtaacc tatctcgaat ttgatcagct tattgaaaca
     1741 gctgatgtca taaccatcca cgccccagca ttaccatcaa caatccacaa attcaataaa
     1801 gatgtttttg ctaagatgaa aaatcgaagc tacttaatca acgctgctag aggcccaatc
     1861 gtttctgaag aagcacttat tgaagctctg aaagaaggtg aaatcgcggg tgcgggatta
     1921 gatgtttttg agaatgaacc acaggtatct gaaggcttga gaagtcttga taatgttatc
     1981 atgagtccac atgctgggac aggtactatt gagggacgta gaactttggc tgaagaagca
     2041 gctgacaaca tcattgcctt ctttgatggc aagccacaaa atattgtcaa taagtaattg
     2101 gctagtgagt gcagagccat cttttgaaaa aatgggacct cccatttttt tacgggtaaa
     2161 tgtaagcgct taataattta ataaaggtga acttatgcta gaagctttac ataaattctt
     2221 tgaagttttt ggtgcagcaa ctgttgtacc cgctatgatt tttattgtca gcctttttct
     2281 aaaggtcaat ccaaggcgag ctttctttag cgccttacga gctggtgttg gactcactgg
     2341 ctttggttgg attatatcag cttttgctcc tatggtgact aagtatatcc agcagatggt
     2401 taaaactact ggtctccatt taccaattgt agatattggt tggcaggctg gttctctcac
     2461 cgccttttct tctgagattg gcctctcttt ctttgttttt ggcttgctaa ttgaattggg
     2521 gcttttcttg cttgggatta cccgagtatt tgtcccatct aatctttgga ataactttgg
     2581 ctatatgatt tggggtacaa tggcctatgc agcaacaggt aattttatat tgtctttcgc
     2641 atttatggtt tttgtcttac tctattcatt agtgatgtca gaagtattgg cagatcgatg
     2701 gtcggagtat tacggtgtta aaaatgcaac gatcaactcg attcataata ttgaaaccct
     2761 tattccagct cttattcttg atcctctttg gaacctactt ggtgtcaata aggtaaagtt
     2821 gaatccagaa tcattaaaga ctaagttagg gatatttggt gagccaatga cacttggttt
     2881 tatacttggt gtgattattg gtgtattagg tagtttacgt aaccttgcaa gtattgatac
     2941 ttggggaggt atacttggtt ttgcagttgc tcttgcagcg gttatgacta tttttccttt
     3001 gattacgggg gtatttgcta gtgcttttgc tcctcttgct gaagcagttg aacgcaataa
     3061 gaaaaaagaa agtcaagctg agcaaggagc actggataaa aaacgttggt ttattgcagt
     3121 agatgacggt gttggttttg gagaaccagc aactattatc gcaggtctca ttttagtacc
     3181 aataatggtt gttatttcct tgattttacc tggaaatgaa gctttaccag tcgttgacct
     3241 tattgctatt ccttttatga ttgaagctat gatagctgtg tcaaaaggaa atatccttaa
     3301 agctattttg aatggaatca tatggttttc actaggtctt tatgctgcta gtgccctagg
     3361 tccaatctac acagaagctg ttaagcattt tggaactgct ttaccagcag gggtaacttt
     3421 gattatgagc tttaatctcc tagctcatcc tttgacagct ctagtcttct ttgcttggat
     3481 atctgggaat ccactctata ttggtgtgac agttattatc tatcttgctt tcttgttctt
     3541 gcttcgtact cgtcgtgaat ctatttacgc ataccttaaa actatggctg ataagaatag
     3601 cggatatgaa ggtagttgtg aaaaaatctt tattgctgaa gattaaaaat ctgttggagg
     3661 aatactcatg aaaaaaattg atggacatgc tcatattgtt gatacaattg caggatttaa
     3721 tggtaaaggg cgtcttaatg cccttggaaa cggttatgct atttgggatg atggtgattt
     3781 aattaagctc atcccagacg aatacggtga caaagcattt acagctgaat cttttttgaa
     3841 gtatatggat gctaatggtg tagataaagc agtcattttg caaggtcatc ttaatggtta
     3901 tcaaaattat tatacgcatt tagctattaa acgttatcca gaacgtttta caggcgcatt
     3961 ctctgttgat ccatttgcag ataatgcaat gcaaattgta aaacgtcatg ttgaagtcct
     4021 tggttttcga gctattaaat ttgaaattag tcaaggaggg ggtatccatg gataccgtgg
     4081 acaaaaaacg cctttccgtc ttgatacaga cccacatgtg agtcgtattt tgacttatct
     4141 gttagactat ccaggatttg tagttacagt tgattatggc aattgggatc aaattagtca
     4201 ccaacctgat gctattgcta atttagctcg cctttatacg agtctcgatt ttgttgtttg
     4261 tcacctctct tttccacatg ttgagcattc aaatcgattg cgtgctgaac ttaatatgtg
     4321 gaaaaacttc aacaatatct atactgatat ttcagctatt caagatattg atgccccaga
     4381 tagttttcct tttccaaaat cagaacaaaa tgtacgtatt gctaaagaag ttcttggggc
     4441 taagcgtatc atttggggaa ctgattctcc atggtcagcg accttcaata cttacgaaga
     4501 attagctact tggctagaga atgttgatat tttcagtcaa gaggaactag aagatgttat
     4561 gtacaataat gctgagcgtg tttactttaa agaagaacat gtaaaggcta acagagcagg
     4621 tatagatgat acaaccaaag agctcaatct ttattagaag agttttcaag gtacaagagt
     4681 ttcttgtacc tcctttttaa atcaacaatg aggagacaaa ttatgactta ctaccttagc
     4741 attgactacg gcggtacaaa taccaaggcg cttatttttg acaaattagg acaccaaatc
     4801 gctgtttcga gttttgaaac tttaaaaaat gaggctcaat ctggtcatcg tcaagtaaac
     4861 cttgttaaaa cgtggaatgc tataacttct gctattagag aggttattca aatctcaaaa
     4921 ctcagccctg agcagattag tgcagtagca tgtattggac atgggaaagg tctttatctg
     4981 ctagataata agttggagcc acttgaacaa ggaattttgt ctacagataa tcgtgccaaa
     5041 gatttggcgc aatatttcga atctaaactt gataatattt gggagttgac tcggcaacat
     5101 attttccctt cacaaagtcc agttatttta cgttggctta aagattatca gcccgaaacc
     5161 tataaatcaa taggggcggt cctttctgca aaggacttta ttcggtataa gcttacagga
     5221 aaagtacagc aagaatatgg tgacgcttca ggtaatcatt ggataaattt tcaaacagga
     5281 acttatgatc cagctatttt agattttttt ggcattagag agatagaaaa ctcacttcct
     5341 gaacttatag atagtgcaga tttagttcct gggggaatta gttctcaagc agcaaaagag
     5401 actggtcttg tagaaggaac ccccgttgtt ggagggctct ttgacatcga tgcttgtgct
     5461 cttggatcag gtgttttaga gtcagatact tttagtgtta tttcgggaac ttggaatatt
     5521 aatacttatc caagtttaaa accagcaaag caagatagtg gtcttatgac ttcctatttt
     5581 ccagatcgtc gttatctctt agaggcaagt agccctactt ctgcagggaa tcttgatttt
     5641 atgttaaaaa tgctcatgca tcaagaaatt gataacgcta aatctagtgg aggttctatc
     5701 tatgataatt tagaagaatt tctcactcat actgatgcca cacatcatgg acttattttc
     5761 tttccgtttc tttacggtag taacacatca caagatgcta gcgcttgctt ttttgggcta
     5821 acaactaaat cgacgaaatc tcagatgata cgtgcggtat atgaaggtat tgcgtttgca
     5881 cataagcagc atatcactga tttaataaaa agtaggggcg gtgtgccaaa aataattcgt
     5941 ttctctggcg gagctaccaa ctcaccagca tggatgcaaa tgttttctga tatcttaaac
     6001 cttcctatta aaacagtaga aggcacagaa ttaggaggat taggaggagc tattttagca
     6061 cgtcatgctt tagataagat ttcgttaaag gaagcagtcc aagatatggt tcgtgtaaaa
     6121 gctatttata aacctcaatt atccgaagta aaggggtacg aaaaaaaata tcacgcctac
     6181 caaaaattat tggaaacact ggatcctatt tggtcggaac tcggtcatct gaataagtag
     6241 ggatatcaaa ggagttgtta tgaaacaaga agatattatt atcaatagta ttgtattcaa
     6301 aaaacagtta gacaaaggtt ttacacaagt tcagctcatt aagttagtaa aggatttagg
     6361 attttcacga tttgagatac gtcaagaatt gcttcaagat ccagatagag agctacccgc
     6421 tttaaaagca gaggctgatt tttatgatat caatctttac tatagtgcta atgaagacct
     6481 tatcaaagga gggaaagtta atccttatct taataaaggc cttaaagaag catcacagtt
     6541 aggagctcct ttcattaagc taaatgttgg acaaaccaga aacttaagta aagaagagtt
     6601 agaacctctt aaagagatat tgaaaagcca aactattggt ataaaggtgg aaaataatca
     6661 agaccctaaa gcagctacag tggagaattg ccaatatttt atgactctag tgaaagaatt
     6721 acaaatacca ataagttttg tttttgatac tgctaattgg gcctttatca accaagacct
     6781 ataccaagcg gtaaataact tagcttgtga taccacctac cttcactgta aaaattttat
     6841 acaggtagct ggaaagcccc acctctcgaa gagcctcttc gaaggagaga ttaatctgac
     6901 tgatttgtta aagagtttct ctaattgtga gtatctagct ctagaatacc ccacagagtt
     6961 ggagatatta aaaagggatg ttcaacgttt aatctctatc agtaatagtc aaaactttta
     7021 atgaaaaggc tttaaaaaga tgttataata agtctgtatg aattcgagta gaaaagaggt
     7081 atattcttga ttattctcga taaaaaaagt tatgacctcc tcttttacct attgaaatta
     7141 gaggaacctg aaacagttat ggcaattgcc aacgcactta atcagtctag acgtaaagtg
     7201 tattatcact tagagaagat aaacgatgca ctgcctagcg atgtgcctca gattgttagt
     7261 tatccacgtg taggaatctt gctaacagaa aaacaaaaag cagcctgtcg tcttttatta
     7321 gatgaagtga ctgattatag ttacgtcatg aaaagtagtg agaggttgca gctgtcttta
     7381 gtatctatcg tagtagctaa ggaccgcata acgattgata ggttgatgca actaaacgat
     7441 gtttctcgaa atactatctt aaacgattta aacgaattaa gaagtgagtt agcagagaaa
     7501 gaatataatt tacagttaca atcaacaaaa tgtcgtggtt attttttaga tggtcatcca
     7561 ttgtccatta tccagtactt atataagctc ttagatgata tctaccataa tggaagtagt
     7621 agttttatag acctttttaa tcataaactg tctcaagctt ttggtgccag cacttatttt
     7681 tctaaagagg ttcttgatta ttttcatcat tatctcttca tttctcaacg aagtctaggt
     7741 aagaaaatca acagtcaaga tggtcagttt atgattcaga ttttgccttt tatactaatg
     7801 gcttatcgta agatgcgatt aagtcctgaa gtacagacct ctcttaatag tgattttagc
     7861 ttggtttggc aacgtaagga atatgagatt gctaaagagt tggctgatga gctggaagaa
     7921 aattttcagt tatcactgga tgagattgaa gtgggactag tagccatgct tatgcttagt
     7981 ttccgcaagg accgtgacaa tcatttagag agccaggatt atgatgatat gcgagctact
     8041 ctaaccagtt ttttgaaaga attggaagaa cgatatcacc ttcactttgt tcataaaaag
     8101 gacttactaa gacaacttct tactcactgc aaggcactct tatatcgtaa acgttatggt
     8161 attttttctg ttaatccttt aacagagcat attaaagaca aatatgaaga actttttgcc
     8221 ataacctcgt cttctgtaaa gcttttagag aaagcttggc aaatcaaatt gaccgatgat
     8281 gatgtagcat atctaacgat tcatttagga ggggaacttc gtaatagtca acaatctcct
     8341 aataaactta agttagttat tgtatctgat gaaggaatag cgattcagaa acttctttta
     8401 aagcaatgtc aacgctactt aacaaatagt gatatagaag ctgtttttac aaccgaacag
     8461 tatcaaagtg tgagtgatct tatgcatgta gatatggttg tctctactag tgacgcttta
     8521 gaatctcgtt ttccgatgtt agtagttcac cctgttttga cagatgatga tattattcgc
     8581 ttgattcgct tttctaaaaa aggtaactgt gcaaatagta atcaatttac caatgaactt
     8641 gaaaaaacaa ttgctcaata tgtcaaggaa gatagtgaac gctacgtgct gaaatctaag
     8701 attgagaaac ttattcatca agaattgctc caagacgtcc ttccccttca aagtacagtt
     8761 tgttaaaata taattttatg gtagcctctc agcttttctg aagacagaaa aagaagctgg
     8821 ggcttttttg gtgcttttaa acaatgtgct agatatttaa tcgcaatgag ttcctctatg
     8881 acatgataat taaagttgag aaagctagta cttttaatgg gagtactttt tatttgtgct
     8941 tattttaatt aaaattatga gtctaatttg gaacagacag cccagttttg aacacataaa
     9001 tgtgtgacac ttggtctttt tttcaaaagt atttgaactt atactagtag atgtaagaaa
     9061 caattaatca ggaggaaata aaatgcctaa cgttaaagat attactagag aatcatggat
     9121 tttatcaaca ttcccagaat ggggtacatg gctaaacgaa gaaattgaag aagaagttgt
     9181 tgctgagggc aactttgcaa tgtggtggtt aggaaactgt ggtgtttgga ttaaaacacc
     9241 aggtggtgct aatgttgtta tggacctatg gtcaaaccgc ggtaaatcaa caaaaaaagt
     9301 aaaagatatg gttcgtggac accaaatggc taatatggca ggtgttcgta aactccaacc
     9361 aaacttacgc gcacaaccaa tggttattga tccatttgct attaatgaat tggattacta
     9421 tcttgtttca catttccaca gtgaccacat tgatatcaac actgcggcag ccattattaa
     9481 taatcctaac ttagaccatg ttaaatttgt tgggccatat gaatgtggtg aaatttggaa
     9541 aaaatgggga gttccagaag aacgaatcat cgtgattaaa ccaggtgaat catttgagtt
     9601 taaagatatc aaagttactg ctgttgaatc atttgaccgt acttgcttgg tgacacttcc
     9661 agtagatggt gcagaagaac atgatggaga acttgctggt ttagccgtga ctgatgaaga
     9721 aatggctcgt aaagcagtta actatatttt tgaaacacca ggtggaacaa tctatcatgg
     9781 agctgactca catttttcaa attattttgc taaacatggt aaagactaca agattgacgt
     9841 agctattaac aactatggtg ataacccagt tggtattcag gataaaatga cttcaatcga
     9901 tcttcttcgt atggcagaaa atctccgtgc caaagtcatt attcctgtac actacgatat
     9961 ctggtctaac ttcatggctt ctacagacga aatcttacaa ttgtggaaaa tgcgtaaaga
    10021 acgccttcaa tatgacttcc atccatttat ttgggaagtt ggaggtaaat acacttatcc
    10081 acaagataaa gatagaattg aataccatca cccacgtggt tttgatgatt gcttcgaaca
    10141 agaatcaaat attcaattta aagcacttct ataatggcta atgagagact gtatgtcgca
    10201 tgatatgcag tttttcaatt ttaaaatatg ttactaaaat cacaattaag attaggagaa
    10261 ataatgtcag aatttgatac aaaatcatat ttagaaaaac ttgatgcatg gtggagagca
    10321 gctaattata tttctgcagc acaaatgtat ctaaaggata accctctctt gagacgagag
    10381 ctggttgaaa acgacttaaa ggttcatcca attggtcact ggggcactgt acctggacaa
    10441 aattttatct atgctcactt aaatcgcgct attaataaat acgatttaga catgttctat
    10501 attgaaggtc caggtcatgg tggtcaagtt atggtatcta attcatattt agatggttca
    10561 tatactgaat taaacccaaa tattgagcaa acagaggatg gctttaagca gttatgtaaa
    10621 atcttctctt tcccaggtgg aattgcatcc catgctgcac cagaaacacc agggtcaatt
    10681 catgaaggcg gtgaacttgg ttacgcactt tctcatgcga caggtgccat cctagacaat
    10741 ccagatgtta ttgctgcgac tgttattggt gatggtgaag gtgaaacagg accacttatg
    10801 gctggttggc tatctaatac ctttatcaat ccagtaaatg atggtgctgt tctaccaatc
    10861 ttctatttaa atggtggtaa aatccataac ccaactatct ttgaacgtaa gacagatgaa
    10921 gaattatctc agttttttga aggattaggt tggaaaccta tttttgcaga tgttgttgaa
    10981 ctctctgagg atcatgcggc tgctcatgct ttgtttgcag aaaaattaga tcaagctatt
    11041 caagagatta aaaccattca atcagaagca cgacaaaaac cagcagaaga agctatccaa
    11101 gcaaaattcc ctgtcttagt tgcacgtatt cctaagggtt ggactggtcc aaaagcttgg
    11161 gaaggaacac caattgaagg tggcttccgc gctcaccaag taccaattcc agtagatgcc
    11221 caccatatgg aacatgttga ttctcttttg tcatggcttc aatcataccg tccagaagaa
    11281 ttatttgatg aaaatggtaa aatcgttgat gagattgctg ctatttcacc aaaaggtgat
    11341 cgtcgcatgt ccatgaatcc aataaccaat gctggtattg ttaaagcaat ggatacagca
    11401 gattggaaga aatttgctct tgatattaat gttccaggtc aaattatggc acaagatatg
    11461 attgaatttg gaaaatatgc agcagatttg gtagatgcta atccagataa tttccgtatt
    11521 tttggtccag atgaaacgaa atcaaatcgt cttcaagaag tgtttacacg tactagccgt
    11581 caatggcttg gtcgtcgtaa accagattat gacgaagctt taagtccagc tggacgtgtt
    11641 attgattccc aattgtctga acaccaagca gaaggtttct tagaaggtta tgttttaact
    11701 ggtcgtcacg gcttctttgc ttcatacgaa tcattccttc gcgttgtaga ttcaatggta
    11761 acacaacatt tcaaatggtt acgtaaatca aaaacacaca caacatggcg taaaaattat
    11821 ccagcgctta acctaattgc agcttcaact gttttccaac aagatcataa tggttatact
    11881 caccaagatc caggtatttt aactcattta gctgaaaaaa cacctgaata cattcgtgaa
    11941 tatttaccag cagatactaa ctcacttcta gctgttatgg ataaagcgtt taaagctgaa
    12001 gataaaatta atttaattgt gacatctaag caccctcgtc cacaatttta ctctattgct
    12061 gaagcagaag agttagtggc agaaggatat aaggtgattg actgggcttc aaatgtgtcg
    12121 cttaatcaag agccagatgt tgtctttgct gcggcgggaa cagaacctaa cttagaagct
    12181 ttggcagcta ttagcattct tcataaggct ttcccagaac ttaagattag atttgttaat
    12241 gtattagaca tcctaaaact tcgtcatcct tcacaagatg ctcgcggttt atcagatgaa
    12301 gagtttaaca aagtctttac cacagataaa cctgttattt ttgctttcca tggttatgaa
    12361 gatatgattc gagatatttt ctttagtcgt cacaatcata atttgcatac gcatggttac
    12421 cgtgaaaatg gtgacatcac aacaccattt gatatgcgcg tgatgtcaga attggaccga
    12481 ttccatctgg cacaggatgc agctcttgct tctttaggaa ataaagcaca agccttcagt
    12541 gatgaaatga atcaaatggt tgcttatcac aaagactata ttcgtgaaca cggagacgat
    12601 attccagaag tacaaaattg gaaatgggaa aatatcaagt agtcactttt ttcaggccta
    12661 tacttacaaa caaaaacact tttagatttt ttctaagggt gttttgttat tttatagaag
    12721 aatgaccgct ttttcaaaag tattaagtgg tcactt
//
LOCUS       DATRQA010000052        12725 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.052, whole genome shotgun sequence.
ACCESSION   DATRQA010000052 DATRQA010000000
VERSION     DATRQA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12725
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.052"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(150..638)
                     /gene="folK"
                     /locus_tag="VBD27_001064"
     CDS             complement(150..638)
                     /gene="folK"
                     /locus_tag="VBD27_001064"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEO7025085.1"
                     /translation="MTTVYLSLGSNIGDRETFLKQALFSIDHLQKTKVAQISAIYETA
                     AWGNTNQEDFFNICCQVETDLAPFELLDYCQEIEKRLKRVRHEHWGPRTIDIDILLFG
                     NQVINQEDLVVPHPYMTKRAFVLVPLLEIAPQLSLPNGSKLEDYLEKLNLGEVHYLKP
                     VE"
     gene            complement(635..997)
                     /gene="folB"
                     /locus_tag="VBD27_001065"
     CDS             complement(635..997)
                     /gene="folB"
                     /locus_tag="VBD27_001065"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEO7025086.1"
                     /translation="MDKIYLNKCRFYGYHGAFSEEQTLGQVFQVDAVLSLDLAKASQT
                     DDLIDTVHYGEVFDCIKNHVENEQYQLIEKLAGVIVEDIFLQFHPVQAITLKITKDNP
                     PINGHYESVGIELERKRV"
     gene            complement(999..1802)
                     /gene="folP"
                     /locus_tag="VBD27_001066"
     CDS             complement(999..1802)
                     /gene="folP"
                     /locus_tag="VBD27_001066"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEO7025087.1"
                     /translation="MKIGQYDITGKACIMGILNVTPDSFSDGGSYTTIDSALNQVGEM
                     LEQGVAIVDIGGESTRPGAVFVTAEEEIKRVVPMIKAIREVYPDLLLSIDTYKTEVAQ
                     AALDAGVHILNDVWSGLYDGKMLSLAAERNVPIILMHNQEEAVYQDIKKEVCEFLLER
                     AERALEAGVSKDNIWIDPGFGFAKTEEQNLELLKGLEQVCDLGYPVLFGISRKRTVNY
                     LLGGNREVTERDMGTAALSAWAIAKGCQIVRVHNVEVNKDIVTVISQLV"
     gene            complement(1806..2369)
                     /gene="folE"
                     /locus_tag="VBD27_001067"
     CDS             complement(1806..2369)
                     /gene="folE"
                     /locus_tag="VBD27_001067"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEO7025088.1"
                     /translation="MPNQEKMEKAIYQFLEALGENPDREGLKDTPKRVAKMYIEMFSG
                     LNQDPKEQFTAVFSENHEEVVIVKDIPFYSMCEHHLVPFYGKAHIAYLPNDGRVTGLS
                     KLARAVEVASKRPQLQERLTAQVAQALEDALAPKGIFVMIEAEHMCMTMRGIKKPGSK
                     TITTVARGLYKDDRYERQEILSLIQKG"
     gene            complement(2388..3650)
                     /locus_tag="VBD27_001068"
     CDS             complement(2388..3650)
                     /locus_tag="VBD27_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO7025089.1"
                     /translation="MTYQEALEWIHSKLAFGIKPGLERMRWMLEQLGNPQNNLSAIHV
                     VGTNGKGSTTSYLQHIFTNSGYQVGTFTSPYIVDFRERISIDGQMIPESDFIKLVETV
                     RPVVERLHLETNLEPATEFEVITVLMFYYFGNSCPVDIVIIEAGMGGYYDSTNMFKAL
                     AVTCPSIGLDHQEVLGRTYVDIAEQKVGVLKKGVPFVYANDRQDVEEVFQIKAKETHS
                     QTYRLHNDFYIKEEENYFNYIGPQANIDHIQLQMPGHHQVSNASIAITTSLLLRDKYP
                     KLTLQTIKDGLEMTKWVGRTELIFPNVMIDGAHNNESVDALVQVIKKYQQKNVHILFA
                     AINTKPIESMLESLSSIAPVSVTSFDYPKSINLDKYPKAYTRVSDWKKWLHDINLTSD
                     KDFYVITGSLYFISQVRQELLLIKTTTT"
     gene            complement(3652..4539)
                     /gene="rarD"
                     /locus_tag="VBD27_001069"
     CDS             complement(3652..4539)
                     /gene="rarD"
                     /locus_tag="VBD27_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO7025090.1"
                     /translation="MRKDNLGIILGLSAYVLWGLLSLYWKLLSGIEAYSTFAYRIIFT
                     VLTMLIYMLVSGRKTVYLKDLKGLVNNKKSFWTMFVASILISINWLVYIFAVTHGHAT
                     EASLGYYMMPIISILLSVLVLREHLARVVSLAILIAIMGVGILVYQTGHFPLISLTLA
                     LSFGFYGLLKKSISLSSDFSMLVESSFIAPFALIYIVFFAKDFLTDYNILQLVLLSLS
                     GIITAVPLLLFAEAIKRAPLNIIGFIQYINPTIQLLLALFIFKETIVSGEVIGFIFIW
                     LAILVFSIGQVHTMLKKGK"
     gene            complement(4526..5392)
                     /locus_tag="VBD27_001070"
     CDS             complement(4526..5392)
                     /locus_tag="VBD27_001070"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEO7025091.1"
                     /translation="MRIIVPATSANIGPGFDSIGVALSKYLSIEVLEESTEWLVEHNL
                     VNIPKDHTNLLIQTALHVKSDLAPHRLKMFSDIPLARGLGSSSSVIVAGIELANQLGN
                     LALSQKEKLEIATRLEGHPDNVAPAIFGDLVISSIVKNDIKSLEVMFPDSSFIAFIPN
                     YELKTSDSRNVLPQKLSYEDAVASSSVANVMVASLLKGDLVTAGWAIERDLFHERYRQ
                     PLVKEFGVIKQISTQNGAYATYLSGAGPTVMVLCSKEKEQPIVTELSKLCLDGQIQVL
                     NIERKGVRVEKR"
     gene            complement(5394..6677)
                     /locus_tag="VBD27_001071"
     CDS             complement(5394..6677)
                     /locus_tag="VBD27_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEO7025092.1"
                     /translation="MTIKIALLGFGTVAKGIPYLLKENQHKLLSLEGEDIVIDKVLVR
                     DNESRQRFINQGFTYNFVTEINTILQDPQIDIVVELMGGIEPAKTYLSQALGFGKHIV
                     TANKDLIALHGKELMDLADARGLALFYEGAVAGGIPILRTLSHSFASDKMTRLLGILN
                     GTSNFMLTKMFEEGWSYEQALKKAQELGYAESDPTNDVEGIDTAYKATILSQFGFGMP
                     IDFDDVNYKGISSIRSEDVEVAQEMGFAIKLVTDLRETPTGISVDVSPTLISQEHPLA
                     AVNHVMNAVFIESIGIGQSLFYGPGAGQNPTATSVLADIIDISRSIRSQIKIKPMNTY
                     HCPCRLSMQSDIFNEYYLAISLRNAEDSDTLGRYFEQENIGLKNVIEKALDDKQQEIY
                     VLTDEVSQEKITQFIEEFPESGVIQLINVFKVIGG"
     gene            6854..7741
                     /locus_tag="VBD27_001072"
     CDS             6854..7741
                     /locus_tag="VBD27_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO7025093.1"
                     /translation="MAHTPTSHRKPRKRSPWLAIASVFFLLIALIGIFLFFNNRSKQE
                     IKTKTNTSSHRKIVTSIKKKKWVKQKTPVKIPILMYHAVHVMDPSEAASANLIVAPDI
                     FESHIKRLKKEGYYFLAPNEAYRALNENALPEKKVIWITFDDGNADFYTKAYPILKKY
                     KVKATNNIITGFVQEGRESNLNVQQMLEMKQNGMSFQGHTVTHPNLSLLTPELQTQEM
                     TLSKLFLDQKLSQDTLAIAYPSGRYNPTTLDIASQYYKLGLTTNEGVATKDNGLLSLN
                     RVRILPTTSDDDLIKTINQ"
     gene            complement(7764..9311)
                     /locus_tag="VBD27_001073"
                     /pseudo
     CDS             complement(7764..9311)
                     /locus_tag="VBD27_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
     gene            complement(9454..10830)
                     /locus_tag="VBD27_001074"
     CDS             complement(9454..10830)
                     /locus_tag="VBD27_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO7025094.1"
                     /translation="MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHALYKTWRKEDN
                     VEERQNQLHKVADLLRKDRDKYAEVMTKDMGKLFTEAQGEVDLCADIADYYADNGQKF
                     LKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMRVFAPNFIVGNTMLLKHASIC
                     PASAQAFEDLVREAGAPEGAFKNIFASYDQVSNLISDPRVAGVCLTGSERGGASIAAE
                     AGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKY
                     DEFVNMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDT
                     IDHPGNFVMPTVLTNITKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTV
                     FSSDPEHAKKVAAQIETGMTFINSGWTSLPELPFGGIKNSGYGRELSQLGFDTFVNEH
                     LVFTPNSD"
     gene            complement(10904..11911)
                     /locus_tag="VBD27_001075"
     CDS             complement(10904..11911)
                     /locus_tag="VBD27_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEO7025095.1"
                     /translation="MLFKEKIPGLILCFIIAIPSWLLGLYLPLIGAPVFAILIGIIVG
                     SFYQNRQLFNKGIAFTSKYILQTAVVLLGFGLNLMQVMKVGISSLPIIIMTISISLII
                     AYVLQKLFKLDKTIATLIGVGSSICGGSAIAATAPVINAKDDEVAQAISVIFLFNILA
                     ALIFPTLGNFIGLSDHGFALFAGTAVNDTSSVTATATAWDAINHSNTLGGATIVKLTR
                     TLAIIPITIVLSIYHMKQTQKEQSVSVTKIFPKFVLYFILASLLTTIVASLGFSLRIF
                     EPLKVLSKFFIVMAMGAIGINTNVSKLIKTGGKSILLGAACWLGIIIVSLTMQAILGT
                     W"
     gene            complement(12019..12705)
                     /locus_tag="VBD27_001076"
     CDS             complement(12019..12705)
                     /locus_tag="VBD27_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025096.1"
                     /translation="MKKFLKVFLTVLVLILIVGAGGLYFWNNRQSLEGKWRTVSLEKQ
                     VEKEIEQQLGSQAADMGISAADLVKGANMHMNVKNDEAKITVTAQIDEVKFHQAIKTF
                     IDKALEKQLKDQGLTYNDLSEAGKKIFDETKITDKQIDQQIDRSFQSAAQAVGGKYNT
                     NTGEMTLPVMDGKVHRLTSVIKVSHINKKANAFYGNIVKNGEKTAYKKEGSKLILGNE
                     KSYPFMKVTK"
BASE COUNT         4250 a         2599 c         1907 g         3969 t
ORIGIN      
        1 aatcataacc tttatctcaa ttctgtttta ttgtgtttta tagacacaat aaatcggcat
       61 aaaaccgatt tctattatac caaaaatata ggttgttgac gatacattat cacaatttct
      121 gtgtatttta attttaaaat cctaactgac tattcgactg gttttaaata atgaacttct
      181 cctaaattca atttttcgag atagtcttct agcttagaac cattaggtaa acttagttga
      241 ggtgctattt cgagtaacgg aactagaaca aatgctcgct tcgtcatata cggatggggt
      301 actactaaat cttcttggtt aatcacttga ttaccaaaaa gtaagatatc aatatctatt
      361 gtccttggac cccagtgctc atgtctcacg cgctttaagc gtttttcaat ttcttgacaa
      421 taatccaata attcaaaagg tgctaagtct gtctctacct gacaacatat attaaaaaaa
      481 tcttcttggt tagtattccc ccaagcagct gtttcataaa tagcggaaat ttgcgcgact
      541 tttgtctttt gcaaatgatc aattgaaaag agagcctgct tcagaaaagt ttctctatca
      601 ccaatattac tacctaaact caagtaaacg gttgtcatac tcgcttgcgc tccaattcaa
      661 ttccaactga ttcatagtgc ccattaattg gtggattgtc ttttgtaatc tttaatgtaa
      721 tagcttgtac tggatgaaac tgtaagaaaa tatcttcaac aataacacct gctaatttct
      781 ctattaattg atactgttcg ttttcaacat gatttttaat acagtcaaaa acttcaccat
      841 aatgaaccgt atcaattaaa tcatcagttt gtgatgcttt tgctaaatca agtgatagaa
      901 cagcatcaac ttgaaaaact tgccccaatg tttgttcttc cgaaaaagca ccatgataac
      961 cataaaaccg acatttattt aaataaatct tatccatatt acaccaactg actaattact
     1021 gtcacaatgt ccttatttac ttcaacattg tgaacacgta caatctgaca cccctttgct
     1081 attgcccatg ccgataaagc agcagtcccc atatcacgtt cagttacttc cctattacca
     1141 cctagaagat aattaacagt acgttttcga gagatcccaa ataacacagg ataacctaaa
     1201 tcacacactt gttccaaacc ttttaatagc tctaaatttt gctcctctgt tttagcaaaa
     1261 ccaaagccag gatctatcca aatattatcc tttgaaacac ctgcttctaa agcacgctct
     1321 gctctttcaa gtaagaattc acaaacttct tttttgatat cttgataaac agcttcttcc
     1381 tggttatgca tcaaaataat tggaacattt cgttctgctg ctaaagatag cattttacca
     1441 tcatataaac ctgaccaaac gtcatttaaa atatgtacac ctgcatcaag ggctgcttga
     1501 gctacctctg ttttataagt atctattgat aacaaaagat cagggtagac ctctctaata
     1561 gctttaatca ttggaactac acgtttaatt tcttcttcag cagtcacaaa tactgcacct
     1621 ggccgcgttg attctccccc aatatctaca attgccaccc cttgttcaag catctcacca
     1681 acttgattta aagctgaatc aattgtggta taagaaccac catctgagaa ggaatcaggc
     1741 gttacattta aaatccccat gatacaagct ttacctgtaa tatcatattg tcctattttc
     1801 atcttttatc ctttttgtat tagcgataga atttcttgtc tttcatatcg atcgtccttg
     1861 tataaaccac gagctaccgt tgtaatcgtc ttacttcctg gttttttaat gccacgcatt
     1921 gtcatgcaca tatgctctgc ttctatcata acaaaaatac ctttaggcgc taaagcatcc
     1981 tctaaagctt gtgcaacttg tgctgttaaa cgttcctgaa gttgaggacg cttactcgcc
     2041 acttcaactg cacgtgcaag cttactaagt ccagtaacac gtccatcgtt tggtaaataa
     2101 gcaatatgag ctttaccata aaaaggaacc aaatgatgtt cacacatgga atagaatgga
     2161 atatccttga caatcacaac ctcttcatga ttttcagaaa agactgcagt gaattgctcc
     2221 tttggatctt gattaagtcc tgagaacatt tctatataca tttttgccac acgcttcggt
     2281 gtatctttca acccttctcg atcaggattt tctcctaaag cctctaaaaa ttgataaata
     2341 gctttttcca ttttttcttg attgggcatt aatttatcct atccttttta agttgttgta
     2401 gttttgatta atagtagttc ctgacgaact tgagaaataa aataaaggga gccagtaatc
     2461 acatagaaat ctttatctga tgtcagattt atatcatgta gccatttctt ccaatcacta
     2521 actctagtgt acgctttagg atatttgtct aagtttattg atttagggta atcaaagcta
     2581 gtaaccgaaa ccggagcgat actagacaat gattctaaca tactctctat cggtttagta
     2641 ttaattgcag caaatagaat atgcacgttt ttctgctgat atttttttat aacttgaacg
     2701 agagcatcga cactttcatt attatgagcg ccatcaatca ttacatttgg aaagattaat
     2761 tctgtacggc ctacccattt tgtcatttca agcccatctt taatggtctg tagggttaac
     2821 tttggatatt tgtctcttaa taataaactg gtagttatag ctatgctagc attggaaact
     2881 tggtgatgtc ccggcatttg caactgaata tgatcaatat ttgcttgagg acctatatag
     2941 ttaaaataat tttcctcttc ttttatataa aagtcattgt gtaaacgata agtctggctg
     3001 tgggtttcct tggcttttat ttgaaagact tcttcaacat cctgacgatc attagcatat
     3061 acgaatggaa ctcctttttt taaaacacct actttttgtt cagctatatc tacataagtt
     3121 cgtcctaaaa cctcctgatg atctaatcca attgacggac aagtaaccgc taaagccttg
     3181 aacatgttgg tagaatcata ataaccgccc attccagcct caataatgac gatatcaact
     3241 ggacagctat tgccaaaata atagaacatc aaaactgtta taacttcaaa ttctgtagct
     3301 ggttctaggt tggtctcaag gtgcaaccgt tcaacaactg gacgaacagt ctcaacaagt
     3361 ttgataaaat cagactctgg aatcatttga ccatcaatgc taatacgctc tctaaagtca
     3421 acaatatatg gtgatgtaaa tgttccaact tgataaccag aatttgtaaa aatatgttgt
     3481 aaataactag ttgttgatcc tttaccattc gttccaacaa catgaatggc agataggttg
     3541 ttttgagggt ttcctaactg ctctagcatc catctcatac gttccaaacc tggtttaata
     3601 ccaaaagcta atttgctatg aatccattct agcgcttctt gataagtcat cttatttacc
     3661 tttctttaac atcgtatgta cttgtccaat actaaaaact aaaatagcaa gccagataaa
     3721 aataaaacct attacctccc cactaacgat agtttcttta aaaataaaca atgctagcaa
     3781 taattggatg gttggattga tgtattggat aaaaccgata atatttagag gagccctctt
     3841 aatggcttct gcaaataata aaagaggaac tgctgtaatg attcctgaaa gtgagagtaa
     3901 aactaattgt aatatattat aatcagttaa aaaatctttg gcgaaaaaca ctatataaat
     3961 gagtgcaaac ggagcaataa aactactttc taccagcatc gaaaaatcgc tagataaaga
     4021 aattgatttt tttaataacc cataaaaacc aaaagaaagc gctagagtta gtgaaattaa
     4081 aggaaaatga ccagtttgat aaactaaaat tccaactccc atgattgcaa tcaaaattgc
     4141 aaggctaact actcttgcca ggtgttcacg taaaacaagg accgacaaca aaattgaaat
     4201 aatcggcatc atataataac caagacttgc ctcagtagcg tgaccatgcg tgactgcaaa
     4261 aatatagacc aaccaattaa ttgaaattaa aatactggct acaaacattg tccaaaatga
     4321 ctttttatta ttaactaagc cttttaagtc ttttaaatag actgtttttc tacccgaaac
     4381 gagcatataa ataagcatag ttaaaacagt gaatataatc ctataagcaa acgtactata
     4441 tgcttcaatt ccagataata atttccaata aagagataaa agcccccata aaacataggc
     4501 tgacaagcca aggataattc ctaaattatc ttttctcaac tctcactcct tttcgctcaa
     4561 tattaagtac ttgaatctgt ccatctaaac ataatttact taactctgtg acaattggtt
     4621 gttctttttc tttcgaacac agaaccatta ccgtcggtcc tgctccagac aaataagtag
     4681 catacgcacc gttttgagtg ctgatttgtt ttataactcc aaattccttc accaaaggtt
     4741 gacgatatct ttcatgaaag aggtcccttt ctatagccca tccagcagtt accaaatcac
     4801 ctttcaacaa cgacgcaacc atcacattag cgacgctgct tgaagcaact gcatcctcat
     4861 aactcaattt ttgaggtagt acatttcgac tatcacttgt ttttaattca taatttggaa
     4921 taaaagcgat aaaggaacta tctggaaaca tcacttccaa acttttaata tcatttttca
     4981 ctattgatga aataactaaa tcaccaaaaa tggccggtgc tacattatca ggatgtcctt
     5041 ctaatcgggt agctatctct aatttttctt tttgagaaag agccaaattt cctaattgat
     5101 tggctaattc tattcctgca acaattacag aggaagaaga acctaaacct ctggccaaag
     5161 gaatatctga aaacattttt aagcggtgtg gtgccaaatc cgacttaaca tgcaaagctg
     5221 tttgaataag gaggttagta tgatcttttg gaatatttac taaattgtgt tcaactaacc
     5281 actctgtact ttcttctaat acttcaatgc ttaaatactt tgataacgca actcctattg
     5341 aatcaaagcc tggaccaata ttcgcactag ttgcaggtac aattatcctc atttcaccct
     5401 cctattactt tgaaaacatt gattaactga atgacaccac tctcaggaaa ctcctcaata
     5461 aattgagtta ttttctcttg gctaacttca tctgttaata catagatttc ttgttgttta
     5521 tcatccaatg ctttttcaat aacatttttc aaacctatat tttcttgctc aaagtacctt
     5581 ccaagtgtat cactatcttc agcatttctc aaagaaatag ctagatagta ctcattgaag
     5641 atatcagact gcattgacaa cctacacgga caatgataag tattcatagg cttaattttt
     5701 atctgtgatc gaatactacg actaatatcg atgatatcgg ctaaaacaga ggttgctgtt
     5761 ggattttgtc ccgcacctgg tccataaaaa agagactgac caatccctat tgattcaatg
     5821 aatactgcat tcatcacatg attaactgca gctaagggat gctcttgaga aattagtgtc
     5881 ggagaaacgt ctacacttat accagttgga gtttcacgaa gatcagttac caacttaatg
     5941 gcaaagccca tctcctgagc tacttcaacg tcctctgagc gaatactaga aatcccctta
     6001 taattaacat catcaaaatc aataggcata ccaaatccaa attgacttaa gatagtggct
     6061 ttgtaggcag tatcaatacc ttcaacatca tttgtaggat cactttcggc ataacctaac
     6121 tcttgtgcct tttttagagc ttgttcataa gaccatccct cttcaaacat ttttgttaac
     6181 atgaagttgg aggtaccgtt gagaattcct aataaacgtg tcattttatc tgaggcaaac
     6241 gaatgcgata gggtccttaa aataggaatg cctccagcaa ctgctccctc atagaataaa
     6301 gctagacctc tagcgtctgc taaatccatc aactcttttc cgtgtaaagc aatgagatct
     6361 ttattggctg tcacaatatg tttaccaaat cctaatgctt gactcaaata agttttagct
     6421 ggctcaatcc cccccattaa ttccactaca atatcaattt gtggatcttg aagaatagta
     6481 tttatctctg tcacaaagtt ataagtaaat ccctgattga tgaaacgctg gcggctttca
     6541 ttatctctta ctaatacttt atcaattaca atatcttcgc cttctaaaga aagtagctta
     6601 tgttgatttt cttttagcaa atatggaata cccttagcaa ccgttccaaa acctaataaa
     6661 gctattttaa tagtcatgaa tttctcctta gaaattgtct ctaacttcaa gacaaacatt
     6721 ggctatatta taacaaatta ccaatatttt ttatagggta aacgcgtctt gtcctatgaa
     6781 agttcttaaa aagagaatat tatttcagaa aagatgatac aataaaagta ctttaaaagt
     6841 taaggagaaa taaatggcac acacacctac tagtcatcgt aaaccacgaa aacgttcccc
     6901 atggttagct attgcttctg tctttttttt gttaatagct ttaatcggca tatttctatt
     6961 cttcaacaat cgttcaaagc aagaaataaa aacaaaaacg aacacctcta gtcatcgaaa
     7021 aattgtcaca tctatcaaaa aaaagaaatg ggttaagcaa aagactcctg ttaaaatacc
     7081 cattttaatg taccatgctg ttcatgttat ggacccttct gaagccgcca gtgctaactt
     7141 aattgtggca cctgacattt ttgaaagtca tataaaacgt ctcaaaaagg aaggatatta
     7201 cttcttagcc cctaacgagg catatcgtgc actaaacgaa aatgctttac ctgaaaaaaa
     7261 agtcatttgg attacttttg atgatggaaa tgccgatttc tacactaagg cataccctat
     7321 actcaaaaag tacaaggtta aagccactaa taatatcatc actggctttg ttcaagaagg
     7381 acgagaatct aacctcaacg ttcaacaaat gctagaaatg aagcaaaacg gcatgtcatt
     7441 ccaaggacat acggttactc accccaatct ttctctctta actccagaac tacaaaccca
     7501 agaaatgaca ctatcaaaac tgtttttaga tcaaaaactc tctcaagata ctcttgcgat
     7561 tgcttatcca tcaggacgct acaatcctac aactttggat attgctagtc aatattataa
     7621 actagggctt acaacaaacg aaggagttgc aacaaaagat aacggtttgc tttctcttaa
     7681 tcgagttcgt atcttaccaa ctactagcga tgatgatttg atcaaaacga ttaaccaata
     7741 aaaagaagcc ttctggcttc tttttattct tcataagatt taaatggttc atttttacgt
     7801 ggataacgat ctggagaaat cgataagccc atttcttttt tctcatggaa aagctccact
     7861 aatagcgcta gcatcatcaa aatcattacg aatgaaaatg gcagagcgac aataattaaa
     7921 acgttctgca gcgctaacag accaccagac aagagtaaga caatagcaat cactgccaac
     7981 actagccccc aaattacttt agttcgattt tttggattta aattaccatc ttctgtcaac
     8041 atagccaaaa cgtacgttgc agaatctgct gatgtaataa aaaatgaaaa aattaaaatg
     8101 atagcaatag tggataaaag ccaacctaaa gtgtagtgat taaaagtagc aaacaatact
     8161 tcctctgttg caaactttgt caaattaata cccagttgtt gtacctgagt tgataatgtg
     8221 ccaaatgctg caaaccatac aaaactcaat aatgtcggta ttaaaagaac taccgttaaa
     8281 aactcccgaa tgctacgccc tttagaaata cgagcgatga aaactccaac aaaaggagac
     8341 caggaaatcc accacgccca ataaaaaatc gtccaattat caatccaaga acgtttagta
     8401 ttgtcaaaag cagctgcacg aaagctcatt ccaaagaaat tttgtaaata agagcctaaa
     8461 gactctgtta gggtatcaaa aatacgaacc gttggtccca atacaataac taaagctaag
     8521 agggctaccg ctaaaatcaa atttaagttc gataaaattt taacaccttt tcctaaacct
     8581 gataaagctg acataacaaa taaagctgtt gtaataagga taataacaat ttgaacaaat
     8641 gcattattgg gaacacccaa taaaaaactt aaccctccgt tgatttgagc agctccaaat
     8701 ccaagtgtcg tagcaactcc aataactgta gcaacaactg tggtgatatc aacaattttt
     8761 cctagtcaac catctgtctt atcaccgaaa agaggcttta aagtgacaga caagaggtat
     8821 ttctcttgct ttcgaaatcc aaaataagct agagccaaag caaccaatgc atatactgcc
     8881 caagcatgta tcccccagtg aaaaaatgta aaacgaaatg catcagctaa tgctgtttgc
     8941 gattccttag gtgcgccagg tgtcgaaata gcaaaatgcg ataaggggtc agctgctccg
     9001 tagaagacca aaccaatccc catacctgct gaaaacatca ttgctatcca tgatacagtt
     9061 gaatattcag gacgtgaatg aggtcgccct aaacgtattt gtccaagagg actcataata
     9121 aaaaagacac acaaagcaag aattgaagta actaacaaca agtacaacca accgaaattg
     9181 gtagatacct gttcacgcaa gatttgtgtc caagattgaa aaccacgagg aacaataatt
     9241 cctaataaga ctaaaataag cgatacaatt agtgaacctg taaacacagg cgtaatatgt
     9301 tttttactca aaatttcctc ctatactaaa atagtatagc atactttaat aaatactcct
     9361 ctcattttaa tcacctgtat caagacctct ccttgtctcc tcaacaaaaa agaaccagat
     9421 aacattatct ggttcttgtg tttgacttag tgattaatca ctatttggtg taaataccaa
     9481 atgttcgttg acaaaggtat caaatccaag ttggctcaat tcacgaccat atcctgaatt
     9541 tttaatacct ccaaacggta attccggtaa tgatgtccac cctgaattaa taaatgtcat
     9601 ccctgtttca atctgagccg ccactttttt agcatgttct ggatcagaag agaaaacagt
     9661 acttcctaaa ccataactag aatcattagc taaagcaata gcttcttctt cagtatccac
     9721 tttatagata gaggctacgg ggccgaaaat ttcttgatta tagattggat ttgctttagt
     9781 gatattcgtt aaaacagttg gcatgacgaa gtttcctgga tgatctatag tgtcatttcc
     9841 aaaaactacc tcagcaccgt ggtctaccgc caatttaatt tgttttaaaa catcatcctt
     9901 tgcacctgca gatgacagtg gagccaaggt tgtttccgaa tccattgggt caccccattt
     9961 agcagactta aaggtctcaa ctaccatatt tacaaattcg tcatacttat cagccataac
    10021 aatgaaacgt tttgaagatg tacatacttg acctgcatta tataaacgcg caaagaatat
    10081 tgttttacta agtaaatcaa aatcagcatc atctaaaata agaaaagcat cgttcccacc
    10141 taattccata gacgattttt tcaaattttt accagcctca gctgctattg atgccccacc
    10201 acgctcagaa ccagttaaac acacacctgc aacacgagga tctgatataa ggtttgaaac
    10261 ttgatcatac gaagcaaaaa tatttttaaa cgctccttct ggagctcctg cttctcggac
    10321 taaatcctca aatgcttgag ctgatgcagg acaaatagaa gcatgtttta aaagcatagt
    10381 attacccact ataaagttag gggcaaatac acgcataatc tgataaaatg ggaaattcca
    10441 tggctcgaca gcaagtaaaa caccgacagc ttgtttcaaa tagtaagcct ctccatttgg
    10501 actttctaac ggaacaggtt ttaaaaactt ttgcccatta tcagcataat agtcagctat
    10561 gtctgcacac aagtccactt ctccttgtgc ttcagtgaaa agttttccca tatctttagt
    10621 cataacttca gcgtacttat cacggtcctt acgtaataaa tctgctactt tatgcaattg
    10681 attttggcgt tcttcaacat tatcttcttt gcgccatgtc ttgtagaggg catgcgctat
    10741 gtctaatgac tgttcgagat cactgtcact gatattatcg aattcatgca atacttcgtt
    10801 agtgtacgga taaattgttt tatatgccat aatgttccct ccttttgata tcctcattat
    10861 aacatttatg aaatcgatta caaatattta gctttagaca agatcaccaa gttccaagga
    10921 tagcttgcat tgttaaacta actataataa tgcctaacca acacgctgca cctaaaagaa
    10981 ttgattttcc tccagtttta attagtttgc tgacattagt atttatacca attgccccca
    11041 ttgccataac aataaaaaat ttagataaaa ccttaagtgg ctcaaagata cgtaacgaaa
    11101 aacctaatga agcaactatc gttgttagca aagaagctaa gataaaataa agcacaaact
    11161 tcggaaatat cttagtaaca cttacagatt gttctttctg cgtttgtttc atatgataaa
    11221 tagataatac aatcgtaatt ggaatgatgg ctaatgttcg tgtcaattta acaatagtag
    11281 ctccgcccaa agtattgctg tgattgatag catcccatgc agtagcagtt gctgtaactg
    11341 acgaggtatc attgacagca gtccctgcaa ataatgcaaa accgtgatca gagagaccaa
    11401 taaaattccc cagcgttgga aaaattaaag ccgctaagat attgaataaa aaaataaccg
    11461 aaatagcttg tgctacttca tcatctttag cattaataac tggtgcagtc gctgcaattg
    11521 cggatccacc acaaatagat gaaccaacac ctattaaagt agcaatcgtt ttatctaact
    11581 taaataactt ttgcaaaaca taagcaataa tcaatgaaat ggaaatcgtc ataataatga
    11641 tgggtaacga tgatatacca actttcatca cttgcatgag gtttagtcca aaacccaata
    11701 agacaactgc tgtttgtaaa atatatttag acgtaaaagc aataccctta ttaaacaatt
    11761 gtcgattttg ataaaaggat ccaacaatta ttccaatcaa gatagcaaag actggtgctc
    11821 ctattaaagg gagataaagc ccaagcaacc aagatggtat agcaataata aagcataata
    11881 ttagtccagg aattttttcc ttaaataaca tgatagtcct cctcacattg actattatat
    11941 cacaaacttt aaaacgtggc aggaaaaccg aaagaggacc agaatatgct ctgatcctct
    12001 ttcattacta attcacaatt atttagttac tttcataaat ggatatgatt tttcgttacc
    12061 taaaatcaat tttgagccct cttttttata ggctgttttt tcaccatttt tcacaatatt
    12121 accatagaac gcatttgcct ttttattgat atgcgatact ttaataacac ttgttaagcg
    12181 atgtacttta ccatccataa ctggcaatgt catttcacca gtattagtat tatattttcc
    12241 tccgacagct tgtgcagcac tttggaaact tctatcaatt tgttggtcaa tttgtttgtc
    12301 agtgattttt gtttcatcaa aaatcttttt acctgcttca cttaaatcat tgtaagtcaa
    12361 accttgatct tttaattgtt tttcaagagc tttatcaata aaagttttta tagcttgatg
    12421 gaattttacc tcatcaattt gagcagtgac tgttatcttt gcctcatcgt ttttgacgtt
    12481 catatgcata ttagcgccct taaccaaatc agctgcacta atccccatat ccgctgcctg
    12541 acttccaagt tgttgttcta tttctttctc aacttgcttc tctaaagaaa ctgtacgcca
    12601 tttcccctct agtgactgac gattattcca gaaatagaga ccgcctgcac ctacaattag
    12661 aataagtact agtacagtta aaaaaacttt taaaaacttt ttcatgataa aaactcctta
    12721 tgata
//
LOCUS       DATRQA010000053        12400 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.053, whole genome shotgun sequence.
ACCESSION   DATRQA010000053 DATRQA010000000
VERSION     DATRQA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12400
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.053"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            57..1925
                     /gene="glgB"
                     /locus_tag="VBD27_001077"
     CDS             57..1925
                     /gene="glgB"
                     /locus_tag="VBD27_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEO7025097.1"
                     /translation="MSNKDELYTFGIGENFHLQNYLGVHSENGSFCFRVWAPNAENVQ
                     VIGDFTDWRNRPLQMNKIQAGVWEANSLDAREGDLYKYLVTRKGGQVVEKIDPMAVYM
                     ERRPGTASVIKVLRNKKWEDGLWMGRRKRLGFQKRPINIYEVHAGSWKKDDFGHPMTF
                     SQLKDYLIPYLVEMNYTHVEFMPLMAHPLDMSWGYQLMGYFAFEHTYGTPEEFQDFVE
                     ACHKNNIGVLVDWVPGHFIQNDDALAYFDGTATYEYQNHDRAHNYRWGALNFDLGKNQ
                     VQSFLISSALFWIEHYHIDGIRVDAVSNMLYLDYDEGPWEANQFGDNRNLEGYHFLRK
                     LNKVIKERHPNVMMIAEESTASTPITKDLESGGLGFDFKWNMGWMNDILRFYEEDPLY
                     RQYDFNLVTFSFMYIFNENFVLAFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYAYQ
                     MCHPGKKLLFMGSEFGQFLEWKYNDQLEWENLNDDMNQKMQRYTKQLNQFYKDHKCLW
                     RIDDSFDGLEIIDADNKSETVLSFIRKDDKGDLLLCVFNMTPVERPNFTIGVPQAGIY
                     EEVLNTEMEEFGGVWKNHNPVTKTQVATWKDYDHTLSFTLPALGASVWRIKRRLRK"
     gene            1967..3106
                     /locus_tag="VBD27_001078"
     CDS             1967..3106
                     /locus_tag="VBD27_001078"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEO7025098.1"
                     /translation="MKNEMLALIFVGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGINNVGVITQYQPLELNTHIGNGSSWGLDGIDSGVTVLQPYSATEGNRWFQGT
                     SHAIYQNIDYIDRINPEYVLILSGDHIYKMDYDDMLQTHKDNLASLTVAVLDVPLKEA
                     SRFGIMNTDSNDRIVEFEEKPEHPKSTKASMGIYIFDWKRLRTVLIDGEKNGIDMSDF
                     GKNVIPAYLESGERVYTYNFDGYWKDVGTIESLWEANMEYIGEDNKLHSRDRSWKIYS
                     KNLIAPPNFMTEDANVKDSLVVDGCFVAGNVEHSILSTNVQVKPNAIIKDSFVMSGAT
                     IGEGAKINRAIIGEDAVIGDGVVIDGSKEVEVIGYKEVAGVPNED"
     gene            3096..4229
                     /gene="glgD"
                     /locus_tag="VBD27_001079"
                     /pseudo
     CDS             3096..4229
                     /gene="glgD"
                     /locus_tag="VBD27_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
     gene            4226..5656
                     /gene="glgA"
                     /locus_tag="VBD27_001080"
     CDS             4226..5656
                     /gene="glgA"
                     /locus_tag="VBD27_001080"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEO7025099.1"
                     /translation="MKIMFVAAEGAPFAKTGGLGDVIGALPKSLSKKGHDVAVVMPYY
                     DMVDQKFGDQIENLMYFYTDVGWRHQYVGVKRLSQDNVTFYFIDNQYYFYRGHVYGDW
                     DDGERFAYFQLAALELMEKIDFIPDVLHVHDYHTAMIPFLLKEKYHWIQAYNNIRAVF
                     TIHNIEFQGQFGPEMLGDLFGVGAERYEDGTLRWNNCLNWMKAAILYSDRVTTVSPSY
                     ANEIKTPEFGKGLDQIMRMEAGKLSGIVNGIDSDLLNPETDAFLPYHFSKSNLEGKIK
                     NKLALQENLGLPQDKNVPLIGIVSRLTDQKGFDIIASELDNMLQQDIQMVILGTGYHH
                     FEETFSYFASRYPEKLSANITFDLGLAQQIYAASDIFMMPSAFEPCGLSQMMAMRYGS
                     LPLVHEVGGLKDTVVAFNQFDGSGTGFSFNHFSGYWLMQTLKLALEVYNDYPEAWKKL
                     QWQAMSKDFSWDTACVAYEQLYQQLQ"
     gene            5988..6188
                     /locus_tag="VBD27_001081"
     CDS             5988..6188
                     /locus_tag="VBD27_001081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEO7025100.1"
                     /translation="MNLAILALGFAVMGVSIGEGILVANIAKSAARQPEMFSKLQTLM
                     FTGVAFIEGTFFVLFAFTFLVR"
     gene            6220..6936
                     /gene="atpB"
                     /locus_tag="VBD27_001082"
     CDS             6220..6936
                     /gene="atpB"
                     /locus_tag="VBD27_001082"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO7025101.1"
                     /translation="MESTSNPTVSFLGIDFDLTILAMSLLTITIIFILVFWASRKMTI
                     KPKGKQNVLEYVYELVNNTISQNLGHYTKNYSLLMFILFSFVFIANNLGLMTSLKTHE
                     HNFWTSPTANFGVDITLSLLVAFICHIEGIRKKGIGGYLKGFLSPTPAMLPMNLLEEV
                     TNVASLALRLFGNIFSGEVVTGLLLQLAVLSPFTGPLAFALNIVWTAFSMFIGFIQAY
                     VFIILSSSYIGHKVHGDEEE"
     gene            6954..7451
                     /gene="atpF"
                     /locus_tag="VBD27_001083"
     CDS             6954..7451
                     /gene="atpF"
                     /locus_tag="VBD27_001083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO7025102.1"
                     /translation="MSILINSTTIGDIIIVSGSVLLLFILIKTFAWKQITGIFEAREQ
                     KIANDIDTAEQARQQAEAFATKREEELSNAKTEANQIIDNAKETGLAKGDQIISEAKT
                     EADRLKEKAHQDIAQNKAEALADVKGEVADLTVLLAEKIMVSNLDKEAQSNLIDSYIK
                     KLGDA"
     gene            7451..7987
                     /locus_tag="VBD27_001084"
     CDS             7451..7987
                     /locus_tag="VBD27_001084"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO7025103.1"
                     /translation="MNKKTQALIEQYSKSLVEVAIEHKIVEEIQQEVAALIDIFETSE
                     LEGVLSSLAVSHDEKQHFVKTLQTSCSTYLVNFLEVIVQNEREALLYPILKSVDQELI
                     KVNGQYPIQITTAVALSPEQKERLFDIAKTKLALPNGQLVEHIDPSIVGGFVVNANNK
                     VIDASVRNQLHQFKMKLK"
     gene            8003..9508
                     /locus_tag="VBD27_001085"
     CDS             8003..9508
                     /locus_tag="VBD27_001085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO7025104.1"
                     /translation="MAINAQEISALIKKQIEDFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFSNGAYGMAQNLESNDVGIIILGDFSEIREGDVVKRTGKIMEVPVGEA
                     MIGRVVNPLGQPVDGLGEIETTATRPVETPAPGVMQRKSVFEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRKYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYNGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDALGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGAAQIKAMKRVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     NDILAFEEALYDYFDAHYDNLFETIRTTKDLPEEAELDAAIQAFKDQSQFK"
     gene            9524..10405
                     /locus_tag="VBD27_001086"
     CDS             9524..10405
                     /locus_tag="VBD27_001086"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO7025105.1"
                     /translation="MAGSLSEIKDKILSTEKTSKITSAMQMVSSAKLVKSEQAARDFQ
                     VYASKIRQITTNLLKSDLVSGSDNPMLASRPVKKTGYIVITSDKGLVGGYNSKILKAM
                     MDTITDYHTENDDYAIISIGSVGSDFFKARGMNVSFELRGLEDQPSFDQVGKIIAQAV
                     EMYKNELFDELYVCYNHHVNSLTSQVRMQQMLPIKELDAEEASEDRVITGFELEPNRE
                     VILEQLLPQYTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQA
                     AITQEITEIVAGANALE"
     gene            10479..11885
                     /gene="atpD"
                     /locus_tag="VBD27_001087"
     CDS             10479..11885
                     /gene="atpD"
                     /locus_tag="VBD27_001087"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO7025106.1"
                     /translation="MSSGKIAQVVGPVVDVVFASGDKLPEINNALIVYKNGDKSQKVV
                     LEVALELGDGLVRTIAMESTDGLTRGLEVLDTGRAISVPVGKDTLGRVFNVLGDAIDL
                     EEPFAEDAERQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALTPEIVGDEHYEVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAETFTGQPGSYVPVEETVRGFKEILDGKHDQIPEDAF
                     RMVGGIEDVIAKAEKMNY"
     gene            11898..12311
                     /locus_tag="VBD27_001088"
     CDS             11898..12311
                     /locus_tag="VBD27_001088"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO7025107.1"
                     /translation="MAQLTVQVVTSDGIRYDHHASLITVRTPDGEMGILPGHINLIAP
                     LIVHQMKINRSHQEGVDWVAVNGGIIEVNEDQVTIVADSAERARDIDLNRAERAKERA
                     ERALEKAQTTQNIDEMRRAEVALRRAINRISVGKK"
BASE COUNT         3975 a         1941 c         2567 g         3917 t
ORIGIN      
        1 accattgagg aaatattttt gtcttatatt gactagtaga cctgtgaggt gagtagatgt
       61 ccaataaaga tgagttatat acttttggaa taggtgaaaa ttttcatctc cagaattatt
      121 taggcgtaca tagcgaaaat ggaagctttt gtttcagagt ttgggctcct aatgctgaaa
      181 atgttcaagt tattggagat tttacagatt ggcgaaatcg acctctccaa atgaacaaaa
      241 ttcaagcagg agtttgggaa gctaacagtt tggatgcgcg cgaaggtgat ttatataaat
      301 atttggttac tcgtaaaggt ggtcaagtag ttgagaaaat cgatcctatg gcagtttata
      361 tggaaagacg tcctggaact gcaagtgtta ttaaagttct tagaaataaa aagtgggaag
      421 atggtctttg gatgggaagg cgcaaacgtc tgggttttca gaagcgtcca attaatattt
      481 acgaagttca tgctggttca tggaaaaaag atgattttgg acatcctatg actttttctc
      541 agttaaaaga ttacttaatc ccctaccttg ttgagatgaa ttatactcat gttgaattta
      601 tgcccttaat ggcacatccg cttgatatga gttggggata tcaattaatg gggtattttg
      661 cttttgaaca tacctatggt actccagaag aatttcaaga ctttgtagaa gcatgtcata
      721 aaaataatat aggagttctt gtagattggg ttccaggaca ctttattcag aatgacgatg
      781 ctttagccta tttcgatgga actgctactt atgagtatca aaatcatgat cgtgcgcata
      841 actatcgatg gggagctctg aactttgatt taggaaaaaa tcaagtacaa tcctttttga
      901 tttcgagtgc cttgttctgg attgaacatt atcatattga tggtattcgt gttgatgcgg
      961 ttagtaatat gttatatctc gattatgatg aaggaccatg ggaagctaac caatttgggg
     1021 ataatcgtaa tttggaagga tatcactttt taaggaaact taacaaagta attaaagagc
     1081 gtcatcctaa tgttatgatg attgctgaag agtcaacagc ttctacccca ataactaaag
     1141 acttagagtc tggaggattg ggttttgact ttaaatggaa tatgggctgg atgaatgata
     1201 tcctgcgttt ttatgaagaa gatcctctct ataggcaata tgattttaat ttagtgactt
     1261 ttagttttat gtatatattc aatgagaact ttgtactagc tttttcacat gatgaagtag
     1321 tacatggtaa aaagagtatg atgcataaaa tgtggggtga ccgttataat caatttgctg
     1381 gtttacgtaa tttatatgca tatcaaatgt gtcatcctgg gaaaaaactt ctctttatgg
     1441 gaagtgagtt tggtcaattt ttagaatgga aatataatga tcaactagaa tgggaaaact
     1501 taaatgatga tatgaaccaa aagatgcaac gctatactaa acaattgaat caattttata
     1561 aagatcataa gtgcctatgg cgtattgatg attcctttga cgggctagaa attattgatg
     1621 cggataataa atctgaaact gttttatctt ttatacgaaa agatgataaa ggcgatttgt
     1681 tactttgtgt ctttaatatg actcctgtag aacgccctaa ctttactatt ggtgttccgc
     1741 aagcgggtat ttatgaagaa gttttaaata ctgaaatgga agagtttgga ggtgtttgga
     1801 aaaaccacaa tcctgtaacg aaaacacaag tggctacatg gaaagattat gatcatactt
     1861 taagttttac attaccagcc cttggggcaa gtgtttggag aataaaacgt cggcttcgta
     1921 aataagtctg caatttagta acctcatttt gaaaggaaag aataatatga aaaatgaaat
     1981 gttagcactc attttcgtcg gaggacaagg cactcgactc ggaaaattga ctcagagcat
     2041 tgcgaaacct gctgttcaat ttggaggtcg ttaccgtatt attgactttg cgctttcaaa
     2101 ttgtgcgaat tcaggtatta ataatgttgg agttattact cagtatcaac cgctggaact
     2161 caatactcat attggaaacg gatctagctg gggactagat ggtatcgatt cgggagtaac
     2221 tgtattacag ccatattctg caacagaagg taatcgctgg tttcaaggta ccagccacgc
     2281 tatctatcaa aatattgact acattgatcg tattaatcca gaatatgtac tcattctctc
     2341 tggggaccac atctataaaa tggactatga tgacatgcta cagacgcata aagataattt
     2401 agcaagccta acagtcgctg ttttagatgt tcctttaaaa gaagcaagcc gttttggaat
     2461 tatgaatact gattcaaacg accgtattgt cgaatttgaa gaaaaacctg aacatcctaa
     2521 gtcaacaaaa gcttcaatgg gaatatacat ttttgactgg aagagattac ggacagtttt
     2581 aattgatggt gaaaagaacg gcatagacat gtcagacttt ggtaaaaatg ttataccagc
     2641 ataccttgaa tcgggtgaac gtgtttatac ctataatttt gatgggtatt ggaaagatgt
     2701 tggtactatt gaatcccttt gggaagctaa tatggaatat attggagaag ataacaaatt
     2761 acatagtcgt gatcgttcat ggaaaatcta ttctaaaaat ttgattgcac cacctaattt
     2821 catgacagaa gatgcaaatg ttaaagattc tttagttgta gatggatgct ttgttgcagg
     2881 aaatgttgag cactccattt tatcaactaa tgtccaggta aaaccaaatg ctattattaa
     2941 agactccttt gttatgagtg gcgcgactat cggtgaaggt gcaaagataa atcgagctat
     3001 tatcggtgag gatgctgtta ttggtgatgg tgttgtgatt gatggaagta aagaagtaga
     3061 agttattggt tataaagaag tagcgggggt tccaaatgaa gattgataaa tatactgcga
     3121 ttttaggaaa tactgttagt tacccagata tgcacagttt gacggataat cgtccgcttg
     3181 cttctctacc ttttgatggt aagtatcgtt taattgattt tcaattatcc tctttagcta
     3241 atgcaggcgt tagaagtatt tatggtattt ttcgtgggaa taatgttcgc tctgtttttg
     3301 accatattag aagtggtcgt gagtggggct taggtacact gttgagtcac tattctttag
     3361 gattctatga tacagacgat gatactcgta cagtcaatgg ggattattac gattaaattt
     3421 taacttactt gaggcgttcg ggtagcgacc agacgatata tatgagttgt gatatgcttt
     3481 gtaatattga cttaagtcaa gtaatccact tgcataatgc aaatggtgtg caagccaccg
     3541 tggtttataa aaaaatgccg agatcagcta ttgcagaggc taatcaagtc ttgaccattg
     3601 atgaatctga tcatgtagtg agtctaggta aagctgtaga taatgacgat aaccaaaaaa
     3661 tgtctgctga gatttatgtt atcgatacac cttggttaat cgaaaaaatg gaagaggaag
     3721 ctcaaaataa tgaaccacgt aaactccgct tcttacttcg tgatttaatt gttgaaagta
     3781 atgctttggc ttttgaatac actggttatt tgtcaaacat ttcttctatt aaatcctatt
     3841 atgatgctaa catggacatg ttaacaccaa ataaatttta ttcgctcttc ttttcaaacc
     3901 aaaaagttta tactaaggtg aaaaatgagg aagctactta ttttgataaa caatcaaatg
     3961 tctctaattc gcaactagcc tatggaagta ttattaaagg ttatttagac cattctatcg
     4021 tttctagaaa ttgtctcctt gaaaaaggga ctcgagtagt caatagtatt atctttccaa
     4081 aagtaaaaat cggagaaggg gcaaccattg aaaatactat tattgacaaa tgcgttaagg
     4141 ttgcatccgg agttacattg aaaggtagtt tagataaacc gttagtgatt cctaaatttt
     4201 cagagattaa tgaggatatt atacaatgaa aataatgttt gttgcggcag agggtgctcc
     4261 ctttgctaaa acaggtggtt tgggagatgt gattggcgca ctcccaaaat cacttagtaa
     4321 aaaaggtcat gatgtcgctg ttgtcatgcc atattatgat atggtagacc aaaaatttgg
     4381 agaccaaata gaaaacctca tgtatttcta tacagatgtt ggctggcgtc atcaatatgt
     4441 aggagttaag agactttctc aagataatgt tacgttctac tttattgata atcaatatta
     4501 tttttataga ggtcatgtct atggtgattg ggatgatggg gagcgttttg cttatttcca
     4561 attagctgct ttagagttga tggagaaaat agattttatt cccgatgtct tacacgtcca
     4621 tgattatcat acagcaatga ttcctttctt attgaaagag aagtaccact ggattcaggc
     4681 ctataataat ataagagcag tttttactat tcacaatatt gaatttcaag ggcagtttgg
     4741 tccagaaatg ttaggagatt tatttggagt tggcgctgag agatatgaag atggaactct
     4801 tagatggaat aattgtctta attggatgaa agcagcaatt ctttactcag atagagtgac
     4861 aacggtatca ccaagctacg caaatgaaat aaaaactcca gaatttggta aaggtttaga
     4921 ccaaattatg cgtatggaag caggtaaatt atctggaatc gtaaatggta ttgacagcga
     4981 tttgttaaat ccagaaacag atgctttttt gccatatcat ttttcaaaaa gcaacttaga
     5041 gggcaaaatt aaaaataagt tggctttaca ggaaaatttg ggcttgcctc aagataaaaa
     5101 tgtaccatta attggtattg tttcacgact taccgatcaa aaaggatttg atattattgc
     5161 ttctgaattg gacaatatgc ttcaacaaga tattcaaatg gttattttag gaacaggcta
     5221 tcaccatttt gaagaaacgt tctcttattt tgctagtcgt taccctgaaa aactatctgc
     5281 taatattact tttgacttag gattagcgca acagatttat gcggcttcag atatcttcat
     5341 gatgccaagt gcttttgaac cttgtggtct ctcgcagatg atggctatga gatatggtag
     5401 cttacccttg gtacatgaag ttggaggatt aaaagatact gtggtagcct tcaatcaatt
     5461 tgatggtagt ggtacaggtt tttcatttaa tcatttttca ggctattggt taatgcaaac
     5521 tctaaaacta gcattagaag tttataatga ttatcctgaa gcgtggaaaa agttacaatg
     5581 gcaagctatg tcaaaagatt tttcatggga tacagcatgt gttgcttacg aacaactgta
     5641 tcagcaacta caataatcac tttattttag tgattagttg ataagcttat cgaatctcta
     5701 tgctaaacgt ttcttatgta aaaccacttt agtatagaga ttttattatc ttataaggta
     5761 aaatattatt aatgtaaaga gacactatat caaaaataaa gtgttgtcat atcttaataa
     5821 gggattaaat taaagctaaa aaaactaaaa attgttattt gaaaaggatt gtgtaaacca
     5881 tctcaaacta tttcatgaaa acgtaatcaa atgaggaaat tcattgacat gcacaagaaa
     5941 taggaataaa ataacggagt aaatattttt taaaggagaa ttctattatg aatttagcta
     6001 ttttagcact tggtttcgcc gtgatgggtg tttcaatcgg tgaaggaatt ttggttgcaa
     6061 atattgccaa atcagcggct cgtcagcctg aaatgtttag caaactgcaa acattgatgt
     6121 ttacaggtgt ggccttcatt gaaggtacct tcttcgttct atttgcgttt acattcttag
     6181 taagatagac gacaatttag aaaaaaggag ggatgaggat tggaatctac aagtaatcca
     6241 accgtatctt ttttaggcat cgattttgat ttaaccatcc tagctatgtc tctcttgaca
     6301 attactatta tctttatctt agtcttctgg gctagtcgta agatgacaat aaaaccaaaa
     6361 ggtaagcaga atgtcttaga atatgtctat gagcttgtta ataatacaat ctcacaaaat
     6421 ttaggacatt atactaaaaa ttatagttta ttgatgttta ttttattcag ttttgtcttc
     6481 attgctaaca acttaggatt gatgacatca ttgaaaacac atgagcataa tttttggaca
     6541 tctccgaccg ctaattttgg tgtagatatt acattatcat tattagtagc atttatttgt
     6601 catattgaag gtattcgcaa aaaaggaatt ggtggttatc tcaaaggttt cttatcacca
     6661 acaccagcaa tgcttccgat gaatttattg gaagaagtta ctaatgttgc ttcattagca
     6721 ttgcgtcttt ttggtaatat cttttctggt gaagttgtca ctggattact tttacaactt
     6781 gcagttttat caccatttac aggtccacta gcttttgcat tgaatatcgt ttggacagca
     6841 ttttcaatgt ttattggttt catccaagcg tatgtgttca ttatcttgag ctcaagttat
     6901 attggacaca aagttcatgg tgacgaagaa gaataagaaa agagaggaga aatatgtcaa
     6961 tacttattaa tagtacaacg attggtgata tcattatcgt atctggctct gtgcttctct
     7021 tatttatctt aattaagact tttgcatgga aacaaatcac aggtattttt gaagcccgtg
     7081 aacaaaagat agctaatgat attgatactg ctgaacaggc acgtcaacaa gctgaagctt
     7141 ttgcaactaa gcgtgaggaa gaactatcta acgctaaaac tgaagctaat caaatcattg
     7201 ataatgctaa agagacaggt cttgcaaaag gtgatcaaat tatttcagaa gcaaaaactg
     7261 aagctgatcg attaaaagaa aaagcacatc aagatattgc ccaaaataag gcagaagcac
     7321 tagcagatgt taagggtgaa gtagcagatt taacagtttt attagctgaa aaaattatgg
     7381 tatcaaatct tgataaagaa gctcagagta atctcattga tagctatatt aaaaagctag
     7441 gagatgccta atgaataaaa agacgcaggc tctaattgag caatattcaa aaagccttgt
     7501 tgaagttgcc attgaacata aaattgttga agaaatccaa caagaagtag cagctttaat
     7561 tgatattttt gaaacaagcg agcttgaggg tgtcctgtca agtcttgccg tttcacatga
     7621 tgaaaaacaa cattttgtga agacattgca aacatcatgt tcaacttatc ttgttaattt
     7681 ccttgaggtt attgtacaaa atgaacgtga agcacttcta tatcctattt tgaaatcagt
     7741 tgatcaggaa cttatcaaag tgaatggtca atatccaatt caaattacaa ctgcagttgc
     7801 tttgtctcca gaacaaaaag aacgtctttt tgatattgct aaaactaaat tagctttacc
     7861 aaatggacag ttagttgagc acattgaccc atctattgtt ggtggatttg ttgtcaatgc
     7921 aaacaataaa gtaattgatg caagtgttcg taatcaatta catcaattta aaatgaaatt
     7981 aaaatagaaa gtggtgtgac ttttggcaat taatgcacaa gaaattagcg ctttaattaa
     8041 aaagcaaatt gaagacttcc agccaaattt tgacgtcaca gaaactggta ttgttacata
     8101 tatcggtgat ggtattgctc gtgctcgtgg gctagataat gctatgagtg gagaacttct
     8161 tgaattttca aatggtgcgt acggtatggc tcaaaacctt gagtctaatg acgtgggtat
     8221 cattattttg ggtgatttct cagaaattcg tgaaggtgat gtggtaaaac gtactggtaa
     8281 aattatggaa gtaccagtag gggaagctat gattggacgt gtcgtcaatc cgcttggtca
     8341 accagttgat ggactaggtg aaattgagac gacagcaaca cgcccagttg aaacaccagc
     8401 acctggtgtt atgcaacgta aatcagtttt tgagcctctt caaacaggtc tcaaagcgat
     8461 tgatgctttg gttcctatcg gacgtgggca acgcgaattg attatcggtg accgccaaac
     8521 tggtaagacg tctgtagcga ttgatgcaat cttgaatcaa aaaggtcaag atatgatctg
     8581 tatctatgtg gctatcggtc aaaaagagtc tacagtacgt acacaagttg aaactttgcg
     8641 taaatatggt gcccttgatt acacaatcgt tgtgacagct tcagcatcac aaccatctcc
     8701 attacttttc attgctccat atgcaggagt agcaatggct gaggaattca tgtataacgg
     8761 gaaacacgtt ttgattgttt atgatgattt atcaaaacaa gccgtagctt atcgtgaact
     8821 ttcgcttctt cttcgccgtc ctccaggtcg cgaagcttac ccaggggatg ttttctatct
     8881 tcatagtcgt cttttggaac gttcagctaa agtatcagat gctttaggtg gaggttcgat
     8941 tacagcgctt ccgttcattg aaacacaagc aggtgatatc tcagcatata ttgctacaaa
     9001 cgttatttca attactgacg gccaaatttt cttgcaagaa aatctcttta actcaggtat
     9061 tcgtccggcg attgatgcag gttcatcagt atctcgtgtt ggtggtgcag cgcaaattaa
     9121 agcgatgaag agagttgcag gtacgcttcg tctagatctt gcttcttatc gcgaacttga
     9181 ggcattcact caatttggtt cagaccttga tgcagcaaca caagcaaaac taaatcgtgg
     9241 tcgtcgcaca gtggaagtgt taaaacaacc attacacaaa ccattgccag ttgagaaaca
     9301 agttgtgatt ttatatgctc taacacatgg attccttgat gatgtacctg taaatgatat
     9361 tttagctttt gaagaagctt tgtatgatta ctttgatgct cattatgata atctcttcga
     9421 aacaatccgc acaacaaaag atttaccaga agaagcagaa ttagatgctg ctattcaagc
     9481 atttaaagat caatcacaat ttaaataata ggagggttct aatatggcag gctctcttag
     9541 tgaaattaaa gacaagattc tttctacaga aaaaacaagt aagattacta gtgcgatgca
     9601 gatggtctca tctgccaagt tggttaaatc tgaacaagct gctagagatt ttcaagtgta
     9661 tgcctctaaa attcgtcaaa ttactactaa cctcttgaag tcagacttag tatctggatc
     9721 agataaccct atgttagctt cacgtccggt gaagaagaca ggttatattg ttattacttc
     9781 tgataagggt ttagttggtg gttacaattc aaaaatctta aaagcgatga tggacactat
     9841 aactgactat catactgaga atgatgatta tgccattatt tcaattggta gtgttggttc
     9901 tgattttttc aaagcacgtg gaatgaatgt ttctttcgaa ttacgtggtt tagaggacca
     9961 accaagtttt gatcaagttg gtaaaattat tgctcaagca gttgaaatgt acaaaaatga
    10021 gttgttcgat gaattatatg tttgctacaa tcaccatgtt aacagtttaa ctagccaagt
    10081 cagaatgcaa caaatgcttc ctatcaaaga attggatgca gaagaggctt ctgaggatag
    10141 agtgattact ggttttgaac ttgagccaaa ccgtgaagtt atattggagc aattattgcc
    10201 tcaatatact gagagtttga tttatggagc tattattgat gctaaaacag cagagcatgc
    10261 ggctggtatg acagctatgc agactgcaac cgataatgct aaaaatgtta tcaatgattt
    10321 aactattcaa tataaccgtg ctcgtcaagc agctatcaca caggaaatta cagaaattgt
    10381 tgcaggtgct aatgcacttg aataattaat tagtctatag aaaaaatagt gaacaaaggt
    10441 attttaaatc ctttgtatca tatgataagg agaaatacat gagctcaggc aaaattgctc
    10501 aggtagttgg acctgtcgtt gacgttgtat ttgcaagtgg cgacaaactt cctgagatta
    10561 ataatgcatt gattgtctat aaaaatggcg ataagtcaca aaaagtagta cttgaagttg
    10621 ctcttgaact tggtgacggc ctcgttcgta caatagctat ggaatcaact gatgggctta
    10681 cacgtggttt ggaagtatta gatactggtc gcgcaattag tgtgccggtt ggtaaggata
    10741 ctttgggtcg tgtcttcaac gttcttggag atgctattga ccttgaagag ccttttgcag
    10801 aagatgcaga acgtcaacca atccataaaa aagcaccatc gtttgatgaa ttatcaacat
    10861 catcagaaat cttagaaaca ggtattaaag ttattgactt attagcacct tacttaaaag
    10921 gtggtaaagt cgggcttttc ggtggtgcgg gtgttggtaa aaccgttctt attcaagaat
    10981 taatccacaa catcgcccaa gaacatggtg gtatttcagt atttactggt gtaggagaac
    11041 gtactcgtga agggaatgac ctttattggg aaatgaaaga atctggcgtt attgaaaaaa
    11101 cggctatggt ctttggtcaa atgaatgaac caccaggagc acgtatgcgt gtggcactta
    11161 ctggtcttac aatagctgaa tacttccgtg atgtagaagg acaagatgtg cttctcttca
    11221 ttgataatat cttccgtttc acacaagcgg ggtcagaagt gtcagcgctt ttaggtcgta
    11281 tgccttcagc cgttggttat caaccaacac ttgctacaga aatgggacaa ttacaagagc
    11341 gtatcacttc aactaaaaaa ggttctgtta catcaatcca agctatttat gtgccagcag
    11401 atgactatac tgaccctgcg ccagctacag cctttgctca cttagattca acaactaacc
    11461 ttgaacgtaa attgacacaa atggggattt atccagccgt tgacccatta gcttcaagtt
    11521 ctcgtgcact cacaccagaa atcgttggag atgagcacta tgaagttgca acagaagtac
    11581 agcgtgttct tcaacgttac cgtgagttac aagatatcat tgcgatttta ggtatggatg
    11641 aattatcaga tgaggaaaaa acgttagttg gacgtgcacg tcgtatccaa ttcttccttt
    11701 ctcaaaactt caatgttgca gaaacattta ctggtcaacc aggttcatat gttccggttg
    11761 aagaaactgt tcgcggattc aaagaaatcc tagatggtaa acatgatcaa attccagaag
    11821 atgccttccg tatggttggt ggtattgagg atgttatcgc aaaagcagaa aaaatgaact
    11881 attaatgagg tgatgctatg gcacaattga ctgttcaagt agtaacatca gatggtatta
    11941 gatatgacca tcacgctagt ctcattactg ttagaacgcc agatggtgaa atgggaattt
    12001 taccaggtca cataaatttg attgctcccc ttattgttca tcagatgaag attaatcgtt
    12061 ctcatcaaga aggtgtagac tgggtagctg ttaatggtgg tataattgaa gttaatgagg
    12121 atcaggtgac aattgttgcg gattcagcag aacgagcacg tgatatagac cttaaccgtg
    12181 cagaacgggc taaggaacgc gctgagcgtg cattagaaaa agctcaaaca actcagaata
    12241 ttgatgagat gcgtagagct gaggttgctc ttcgtcgcgc aattaaccgt attagcgtcg
    12301 gtaaaaagta gaaaaatggt ctaattagtt ttgtgactaa ttaggctttt ttagtgtata
    12361 atagaaggca tgaatatgat attaaatata gtaactatag
//
LOCUS       DATRQA010000054        12145 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.054, whole genome shotgun sequence.
ACCESSION   DATRQA010000054 DATRQA010000000
VERSION     DATRQA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12145
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.054"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..809)
                     /locus_tag="VBD27_001089"
     CDS             complement(<1..809)
                     /locus_tag="VBD27_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEO7025108.1"
                     /translation="MIRFLILAGYFELSMYLKLSGKLNQYINTHYTYLAYISMVLSFI
                     LAIVQLIIWVKNMKMHSHLHGKIAKSTSPMILVFPVLVGLLVPTVSLDSTTVSAKGYN
                     FPLAAGSTGTVSQDGTRVQYLKPDTSTYFTSSAYEKEMQKELKKYKGSGTLTITTENY
                     MEVMELIYLYPEQFMDRQIQYTGFVYNEPKHEGYQFIFRFGIIHCIADSGVYGLLTTG
                     NQKSYPDNTWVTVRGTIKSEYNQLLQQNLPVLHIEESRQVSKPNNPYVYRV"
     gene            complement(809..1711)
                     /locus_tag="VBD27_001090"
     CDS             complement(809..1711)
                     /locus_tag="VBD27_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7025109.1"
                     /translation="MDIFNQLPDSVLQWFAIFISIIIEALPFVLLGTILSGIIEVFIT
                     PDIVNKFLPKNKFLRVLFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSIRFLILRFVGATIVAIALGVMLAFLVDDNILKEDAKPTHFH
                     DYSDKKWYQKIFLALAHAIDEFFDTGRYLVFGTLIASAMQIYLPTRVLTTIGHSPITA
                     ILVMMLLAFILSLCSEADAFIGASLLSTFGIAPVMAFLLIGPMIDIKNLMMMVNSFKT
                     RFIVQFISVSSLIIIIYCLFVGVI"
     gene            complement(1717..1845)
                     /locus_tag="VBD27_001091"
     CDS             complement(1717..1845)
                     /locus_tag="VBD27_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025110.1"
                     /translation="MAITHKRQDDLESMFASFAKVPKPKKVDSDSKPEQKDDSQKD"
     gene            1978..3021
                     /locus_tag="VBD27_001092"
     CDS             1978..3021
                     /locus_tag="VBD27_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO7025111.1"
                     /translation="MIELGITTFGETTILEETNQSYSHPERIRQLVAEIELADQVGLD
                     VYGIGEHHREDFAVSAPEIILAAGAVRTNNIRLSSAVTILSSNDPIRVYQQFSTIDAL
                     SNGRAEIMAGRGSFIESFPLFGYDLADYDDLFNEKMDMLLAINSATNLDWKGHLTQTV
                     NERPIYPRALQRQLSIWVATGGNVDSTIRIAEQGLPIVYATIGGNPKAFRQLVHIYKE
                     VGSRNGHKPEQLKVAAHSWGWIEEDNQTAIDRYFFPTKQTVDNIAKGRPHWSEMTKEQ
                     YLRSVGPEGAIFVGSPEVVAHKIIGLVEALELDRFMLHLPVGSMPHKDVLNAIKLYGE
                     EVAPIVRKYFEKN"
     gene            3125..5287
                     /locus_tag="VBD27_001093"
     CDS             3125..5287
                     /locus_tag="VBD27_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO7025112.1"
                     /translation="MENLNITQIAIDLGIKASQIEKVLELTDEGNTIPFIARYRKEMT
                     GNLDEVQIKSIIDLDKSMTALSDRKTTVLAKIEEQGKLTQELKKAIEEATKLADVEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNKDNLEEEAQNYLTDGFETTTKALSGAVD
                     ILIEAFSEDNKLRSWTYNEIWNYSSITAVVKDESLDEKQVFKIYYDFSEKISKLHGYQ
                     VLALNRGEKMGVLKVNFEHNLEKMFRFFAVRFKETSQYIDDLIVQTVKKKIVPAMERR
                     IRTELSEGAEDGAISLFSENLRNLLLVSPLKGKMVLGFDPAFRTGAKLAVVDQTGKLM
                     TTQVIYPVPPANQAKIEQSKIELAKLIKEFNIEIIAIGNGTASRESEAFVAEVLQDFP
                     DVSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLAENLDFVVETVVNQVGVNVNTASPALLAHVSGLNKTISENIVKYR
                     EENGQIKSRAEIKKVPRLGAKAFEQAAGFLRIPNAKNFLDNTGVHPESYEAVKKLLDQ
                     LTIKELDDLAKEKLQHLDLIATAESIGVGQETLKDIIEDLLKPGRDLRDDFEAPVLRH
                     DVLDVSDLKVGQELQGTVRNVVDFGAFVDIGVHEDGLIHQSRLIKRKRDKKTRKMPPL
                     PHPSKYLSVGDIVTVWVVEVDAERGRIGLSLIKPDGLD"
     gene            5250..5708
                     /locus_tag="VBD27_001094"
     CDS             5250..5708
                     /locus_tag="VBD27_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEO7025113.1"
                     /translation="MDLVLSSQTDLTNYVKKVSIEDFGCQFLHTAHWNNRLRTTGGRF
                     FPNDRHLDFNPKIYREFGIDIFRKIVRHELCHYHLYIQGKGYRHRDKAFKELLEKVDG
                     LRYTPRVTAVKVNYHHYSCQSCGQVYRRRRRVNLRKFACGYCHGRLIESF"
     gene            5789..6052
                     /locus_tag="VBD27_001095"
     CDS             5789..6052
                     /locus_tag="VBD27_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEO7025114.1"
                     /translation="MKSSFYKQRKGKLVCGVVAGLADKYNWDLALSRVLIALILYFTK
                     FGLLLYILLAVFLPYKEDIIETRRQGPRRRKDAEPVDDDGWFW"
     gene            6280..7215
                     /locus_tag="VBD27_001096"
     CDS             6280..7215
                     /locus_tag="VBD27_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEO7025115.1"
                     /translation="MAVTVQMLVDRLKLNVIYGDEHLLSKRITTADISRPGLEMTGYF
                     DYYAPERLQLVGMKEWSYLMAMTGHNRYQVLREMFQKETPAIVVARDLEIPEEMYEAA
                     KDTGIAILQSKAPTSRLSGEVSWYLDSCLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSQVQLAIYLENFETGKVFDRLGNGNEEIELSGVKVPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKQMGFDATQTFEDRLTHLISQNEVNDD"
     gene            7208..7981
                     /locus_tag="VBD27_001097"
     CDS             7208..7981
                     /locus_tag="VBD27_001097"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO7025116.1"
                     /translation="MINPVAIQLGPFSIRWYAICIVSGMLLAVYLAMKEAPRKNIKSD
                     DILDFILMAFPLSIVGARIYYVIFEWAYYSKHPVEIIAIWNGGIAIYGGLITGAILLV
                     IFSYRRLINPIDFLDIAAPGVMIAQAIGRWGNFINQEAYGRAVKNLNYLPNFIKNQMY
                     IDGAYRVPTFLYESLWNFLGFVIIMSIRHRPRTLKQGEVACFYLVWYGCGRFIIEGMR
                     TDSLYLAGLRVSQWLSVILVIIGIVMIIYHRREQHISYY"
     gene            7996..8394
                     /locus_tag="VBD27_001098"
     CDS             7996..8394
                     /locus_tag="VBD27_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEO7025117.1"
                     /translation="MIEIAVLIIAIAFVVLVLGILFVLKKVSETIEETKQTIKVLTSD
                     VNVTLYQTNEILAKANVLVDDVNGKVSTIDPLFVAIADLSESVSDLNLQARHIGQKAS
                     SATSSVTKAGSALAIGKAASKIFRKKGDKK"
     gene            8391..8822
                     /locus_tag="VBD27_001099"
     CDS             8391..8822
                     /locus_tag="VBD27_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO7025118.1"
                     /translation="MSKLFKTLVISAASGAAAAYFLTTKKGKELRKNAEKFYGEYKEN
                     PEEYHQIAKDKASEYSNLAVDTFKDYKGKFESGELTTEDIVSAVKEKSGEVVDFANDF
                     VNQAKSKFSDEDTAKKEDKAPETKVEDIVIDYKENTEDKEK"
     gene            complement(8863..9138)
                     /locus_tag="VBD27_001100"
     CDS             complement(8863..9138)
                     /locus_tag="VBD27_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEO7025119.1"
                     /translation="MSSPLKQEQTHESKTEQAQPKFQVFQEFDHSAKLRELVFFARIA
                     LFSITTVLISFLLLALSLTPVWSFLLAMLASLAVTSAVSSFIWSLRQ"
     gene            9481..10407
                     /locus_tag="VBD27_001101"
     CDS             9481..10407
                     /locus_tag="VBD27_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO7025120.1"
                     /translation="MEKIILTATAESIEQVKQLLAIGIDRIYVGEENYGLRLPHSFSD
                     DELREIAKLVHDAGKELTVACNALMHQEMMDNIKPFLELMKEINVDYLVVGDAGVFYI
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVETVLAREIPSEELFKMSENLEFPAE
                     ILVYGASVIHHSKRPLLQNYYNFTHITDEKTRERGLFLAEPGDPESHYSIYEDKHGTH
                     IFINNDINMMTKVTELVEHHFTHWKLDGIYCPGDNFVAIAEIFVETARLIENGTFTQD
                     QAFLFDERIRKLHPKGRGLDTGFYDFDPSTVK"
     gene            10537..11823
                     /locus_tag="VBD27_001102"
     CDS             10537..11823
                     /locus_tag="VBD27_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO7025121.1"
                     /translation="MSNVKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGINYAHARDAKVYVAANMVTHEGNELGAGPWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKEMGLTRVVLAREVTMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGQERQSL
                     KGEIPEPFSMSAVDMCMIEHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFEAIKEDLIDELWKVAQRELATGFYYHTPTENEQLFGARRKIPQYKFVGEVV
                     SFDNAKMEATIRQRNVIMEGDRIEFYGPGFRHFECFIEGLRDAEGNKIDRAPNPMELL
                     TITLPNPVKKGDMIRACKEGLVNLYQNDGTSKTIRA"
     gene            11950..>12145
                     /locus_tag="VBD27_001103"
     CDS             11950..>12145
                     /locus_tag="VBD27_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEO7025122.1"
                     /translation="MSEFTFEIVEKLLVLSENEKGWTKELNRVSFNGAPAKFDLRTWS
                     PDHTKMGKGITLSNEEFKVIL"
BASE COUNT         4045 a         1851 c         2449 g         3800 t
ORIGIN      
        1 aagacacgat aaacataagg attattaggt ttagaaactt gacgagactc ttcaatgtgt
       61 aagactggta agttttgttg taacaattga ttatactcgg atttaattgt acctctaaca
      121 gttacccaag tattgtccgg ataacttttt tgatttccag ttgttagtag accataaact
      181 cctgaatcag caatacaatg aatgattcca aagcggaaaa taaattgata tccttcgtgt
      241 ttgggttcgt tataaacaaa cccagtatat tgtatttggc gatccatgaa ttgctcggga
      301 tagagataaa ttagttccat aacttccatg tagttttcag ttgtgatggt taatgtacca
      361 gagcctttat attttttaag ctctttttgc atttcttttt cataggctga agaagtaaaa
      421 taagtgctgg tatctggttt caaatattga acgcgtgtcc catcttgact gacagtacca
      481 gtagagccgg ctgctagagg gaaattatat cccttggcag agactgtggt cgagtctaaa
      541 ctaactgtag gtactaacaa acctactaaa acgggaaata ctaaaatcat tgggcttgtt
      601 gatttagcta ttttaccatg taggtgacta tgcattttca tatttttaac ccaaataatt
      661 agttgtacaa ttgctaaaat aaaagatagg accattgaaa tataggccaa ataggtataa
      721 tgagtattaa tatactgatt tagtttacct gataacttta aatacatgct taattcaaaa
      781 taaccagcaa gaataagaaa tcgaatcatt atattacccc cacaaataaa caatagataa
      841 taataataag agaagataca ctaatgaatt ggacaataaa ccttgtttta aaagaattta
      901 ccatcatcat taaattctta atatcaatca tgggtccaat taaaaggaaa gccatcactg
      961 gcgcgattcc aaaggttgat aataaagaag cacctataaa agcgtcagct tcactacaca
     1021 atgacaagat gaaagcaagc aacatcataa ctaagatggc tgtgataggg ctgtgtccga
     1081 tagtagtaag aactcgagtt ggtaaataaa tttgcatagc tgaagcaatt aaagtgccaa
     1141 atactagata gcgtcctgtg tcaaaaaatt catcgatggc atgagctaag gctagaaaga
     1201 ttttttgata ccacttctta tcagaataat catgaaaatg agtaggttta gcgtcttctt
     1261 ttagaatatt atcatctact aaaaaggcaa gcatgactcc taaagcaatg gctacaatgg
     1321 tagcacctac aaagcgtaat atcaggaagc gtatagaatt tccaaatgct gagtaggtcg
     1381 caaataaaac aataggatta ataataggag cagtagcaag aaacggaact gccgtgtatg
     1441 aaggcacttt cttttcaagg aatcgattaa taataggaat gataccacat tcgcaagaag
     1501 gaaagacaaa gccaacgaag gttccaaata agactctgag aaatttattt ttcggtaaga
     1561 acttattgac tatatcaggt gttataaaga cttcaataat tcctgataaa atagttccaa
     1621 gcaaaacaaa aggaagtgcc tcaataataa tggaaatgaa aatggcaaac cattgaagga
     1681 cactatccgg taattgatta aagatatcca tagaacttaa tctttctgag aatcatcttt
     1741 ctgttcaggt tttgaatcag aatcaacttt tttaggtttt ggtactttag caaaactagc
     1801 gaacatactt tctaagtcat cttgacgttt gtgcgtaata gccattaatg gcacctcttt
     1861 ctttatataa taacctatta tactatatta gcttattttt aacataaaaa aacaaatcga
     1921 ttggaaatga tactatatag tgataaaatg ttattagtag attgatgagg taagattatg
     1981 attgaattag gtattacaac attcggagag acaacaatcc ttgaagaaac aaaccaaagc
     2041 tattcacatc ctgagaggat tcgccaatta gttgctgaga ttgaactagc tgatcaagtt
     2101 ggtttagatg tatatggtat tggagagcac catcgtgaag attttgcggt ctctgcaccc
     2161 gaaattatcc tagcagcagg agcggttaga actaataata tccgtttatc tagtgcagta
     2221 acgattctct cttccaatga tcctattcgc gtctatcagc aattttcaac gattgacgca
     2281 ctttcaaatg gtagagcaga aattatggca gggcgtggtt cctttattga gtcttttcca
     2341 ttgtttggat acgatttagc ggattatgat gatttattta atgaaaaaat ggatatgttg
     2401 ttagcaatta actcagcgac aaatctcgat tggaaaggtc atttgacaca aacagttaat
     2461 gagcgaccaa tttatccaag agcattacaa agacagttat caatatgggt ggcaacagga
     2521 ggaaatgttg attctacaat tcgtattgca gaacaaggtt tgccaattgt ttatgcaact
     2581 attggtggga atcccaaagc ctttcgtcaa ttggtccata tttataaaga agttggtagt
     2641 cgtaatggac acaaaccaga acaactaaaa gttgctgctc actcttgggg atggattgaa
     2701 gaggataatc aaaccgctat tgaccgttat tttttcccta cgaaacagac cgtcgataat
     2761 attgctaagg gacgccctca ttggtctgaa atgactaaag agcagtattt acgttcagta
     2821 ggtccagaag gtgctatttt tgtaggaagt cctgaagtgg ttgcacataa aattatagga
     2881 cttgttgaag cgcttgagtt agaccgtttt atgttacatc taccagttgg ctctatgcct
     2941 cacaaagacg tcctaaatgc catcaaactt tatggtgagg aagtagcacc aattgttcga
     3001 aaatattttg aaaagaatta aagcagacct ggtctgtttt ttattgttag attataaaaa
     3061 cgctatcttg aaatcgcttt ttaaaactct tcgtaagtca aaaatatgct ataataaaag
     3121 cgttatggaa aatttaaata ttacacaaat tgctattgat ttaggtatta aagcgtcaca
     3181 aatcgaaaag gttttagaat taacagatga aggaaatact attcctttta ttgcccgtta
     3241 tcgtaaagaa atgacaggta atttggatga agttcaaatc aaatcaatta ttgaccttga
     3301 taagagtatg acagctcttt cagatcgtaa aactacggtc ttagccaaaa ttgaagaaca
     3361 aggaaagttg actcaagaac tcaaaaaggc tattgaagaa gcaactaaac tagcagatgt
     3421 tgaggaactc tacctacctt ataaagaaaa acgtcgtacg aaagctacga ttgcacgtga
     3481 agcaggtctt tttcctttag cacgtcttat tttacaaaat aaggataacc ttgaggaaga
     3541 agctcaaaac tatcttacag atggttttga gacaactacg aaggcccttt ctggagcagt
     3601 agatattctt atcgaagctt tttctgagga taataaatta cgttcatgga catataatga
     3661 gatctggaac tacagctcaa tcactgcagt tgttaaagat gagtcacttg atgaaaaaca
     3721 ggtcttcaaa atctactatg acttttctga aaaaataagt aaactccatg gctatcaagt
     3781 tttagcattg aatcgtggtg agaaaatggg tgttcttaag gttaattttg agcataacct
     3841 tgaaaaaatg tttcgctttt ttgctgttcg atttaaagaa actagccaat atatagatga
     3901 ccttattgta cagactgtca agaaaaaaat tgttccagct atggaacgcc gtatccgtac
     3961 agaactaagt gaaggagcag aagatggtgc aatttcactg ttttcagaga atcttcgtaa
     4021 cttattgtta gtctcaccgc ttaaggggaa aatggtgtta ggttttgacc ctgcctttcg
     4081 tacaggagca aaattagcag tagtagacca aactggaaaa ttaatgacaa cacaagtgat
     4141 ttatccagtt cctccagcta atcaagcaaa aatcgaacaa tcaaaaatag agttagcaaa
     4201 acttatcaaa gaattcaata tagaaattat tgctataggg aatggaacag cgagtcgtga
     4261 atcagaagca tttgttgcag aagttttaca agattttcca gacgtatcct atgtgattgt
     4321 taacgaaagt ggtgcctctg tttattcagc ctcagaattg gcacgtcatg aatttccaga
     4381 cttaacagtt gaaaaacgtt cggctatctc aattgcaaga cgtctgcaag acccgttagc
     4441 agaactagtc aaaattgatc caaaatctat tggtgtgggt caatatcaac atgatgttag
     4501 tcaaaaaaaa ttagcagaaa atcttgattt tgttgttgaa actgtagtta accaagtagg
     4561 tgttaatgtt aatacagcaa gtccagcctt gttagctcac gtttcaggtt taaataaaac
     4621 aatatctgaa aatattgtta aatatcgtga agagaatggg caaattaagt cacgtgctga
     4681 aattaagaaa gtacccagat taggagcaaa agcttttgag caagctgctg gattcttacg
     4741 tattcctaat gctaaaaatt tcttggataa tacaggcgtt caccctgagt cttatgaagc
     4801 tgttaaaaag ttgcttgacc aactgactat caaagaatta gatgatcttg caaaagagaa
     4861 gttacaacac cttgacttaa ttgcaacagc agaaagtatt ggggtaggtc aagaaacatt
     4921 aaaagacatt atagaagatc ttttgaaacc aggtcgtgat ttgcgtgatg attttgaagc
     4981 gcctgttttg agacatgatg ttttagatgt cagtgattta aaagttggac aggaattgca
     5041 aggaactgtt cgaaacgtcg ttgattttgg tgcttttgtt gatattggtg ttcatgaaga
     5101 tggtctaatc caccagtctc gccttattaa gcgaaaacgc gataaaaaaa ctagaaaaat
     5161 gcctccttta ccacatccaa gcaaatatct ctctgttggt gatattgtaa ctgtttgggt
     5221 tgtagaggta gatgcggagc gtggcagaat tggacttagt cttatcaagc cagacggact
     5281 tgactaatta tgttaagaag gtgtctatag aagattttgg atgtcagttt ttacatactg
     5341 cacattggaa taatcgctta agaacaaccg gaggacgctt ctttccaaac gatagacacc
     5401 ttgactttaa tcccaaaatt tatcgtgagt ttggtattga tatttttcgt aagattgtaa
     5461 gacatgagct ttgccattat catctctata tacaaggtaa aggatatcga catagggata
     5521 aagcttttaa agagttacta gaaaaagtcg atggcttacg atatacccct agagttacgg
     5581 ctgttaaagt gaattatcat cattactctt gccaatcttg tggtcaagta taccgacgta
     5641 ggagacgtgt taatttacgc aagtttgctt gtggttattg tcatggtcgc ttgatagaga
     5701 gtttttaaaa tcagtcgtaa ggcctattta attttactgt caaagtagtt ataattaaaa
     5761 tatagagtag atagaaagaa ggataggtat gaaatcaagc ttctacaaac aacgaaaagg
     5821 taagttggtc tgcggtgttg tcgccggtct agctgataag tataactggg atttagctct
     5881 ttcacgggtg ttgattgcac tgattttgta ttttaccaaa tttggtcttt tgctatatat
     5941 tttattagct gttttcttac cctataaaga agatattatt gaaacaagaa gacaaggtcc
     6001 cagaagacgt aaagatgctg aacctgttga tgatgacggt tggttctggt aattcatata
     6061 aaggagattt gctagacgaa tctccttttt tcttagcttt atttagacca ttttttcaaa
     6121 atatcttctt tttttcagaa aaacgcgtta gcgcttactt ttagtaaaaa gtatgatata
     6181 atgtgggtgt aaattaatga aagtgtttag tacctttgct caactttaaa ttgtagggta
     6241 tagcaatagg gatattcact tttataagaa ggagtaagta tggcagttac tgttcaaatg
     6301 ttagttgacc gcttaaagtt aaatgtcatc tatggtgatg aacatctatt atctaagcgg
     6361 attacaacag ctgacatttc acgtcctggt ctagaaatga cagggtattt tgattattat
     6421 gcaccagagc gtttgcaact tgttggtatg aaagagtggt catatttaat ggctatgaca
     6481 ggacataatc gttatcaagt gttacgtgag atgttccaaa aagaaacacc agctattgtt
     6541 gtagcacgtg atttagaaat tccagaagag atgtatgagg cggctaaaga taccggtatt
     6601 gctattcttc aaagtaaggc ccctacaagt cgtttatcag gtgaagtttc ttggtattta
     6661 gattcttgct tagctgagag gacaagtgtt catggtgtct tgatggatat ctatggtatg
     6721 ggtgttctga ttcaaggaga ttccggtatt ggtaagagtg agacaggact agagctagtt
     6781 aagcgaggac atcgtttggt agcagatgac cgtgttgatg tttatgctaa agacgaagag
     6841 acgctttggg gagagcctgc tgagatatta cgtcatttac ttgaaattag aggtgtggga
     6901 atcattgata ttatgagcct atatggagct agtgcggtta aagattcatc gcaagtgcaa
     6961 ttagctatat accttgaaaa ctttgaaact ggaaaagttt ttgatagact cggaaatggc
     7021 aatgaagaga tcgaattatc tggagttaag gttcctcgga tacgtattcc tgttaaaaca
     7081 ggccgaaacg tgtcagtagt tattgaagca gcggctatga atcacagagc taaacaaatg
     7141 gggtttgatg ctacccagac ttttgaggat cgtttaacac atttgattag tcagaacgag
     7201 gtcaatgatg attaatccag tagcaataca attaggcccc ttttctattc gctggtatgc
     7261 tatttgcata gtgtctggta tgttgttagc agtttactta gcaatgaaag aggcccctcg
     7321 taagaacatt aagtcggatg atattctaga tttcattctc atggcttttc cactatccat
     7381 tgttggagct cgtatttatt atgttatctt cgaatgggct tattactcta agcacccagt
     7441 agagattatc gccatttgga acggtggtat tgctatttac ggagggttaa ttaccggagc
     7501 cattctcctt gtgatttttt catatcgtcg cttgattaat cccattgatt ttctggatat
     7561 tgctgctcca ggagtgatga ttgcccaagc cataggtaga tgggggaatt ttattaatca
     7621 agaagcatac ggtagggcag taaaaaatct gaattactta ccaaacttta taaaaaatca
     7681 aatgtatatt gatggagctt accgagtacc tacattttta tatgagtctc tttggaactt
     7741 cctaggtttt gtcatcatca tgtctattcg tcatcgaccg cgcactttaa aacaaggtga
     7801 agtggcatgt ttttatttag tttggtatgg atgtggacgg ttcattattg aagggatgag
     7861 gacagatagc ctttatttgg cgggtttgcg cgtgtcacaa tggttatcag ttattttagt
     7921 tattatcgga attgtcatga ttatttatca tcgtcgtgaa caacatattt catattatta
     7981 aacagaggag gcattatgat agaaattgct gtattaatta ttgccattgc gtttgttgtt
     8041 ttagttttag gaatactttt cgttctgaaa aaagtatcag aaacgattga ggaaaccaag
     8101 caaactatta aagtattaac aagcgatgtt aacgtgactc tttaccaaac taatgaaatt
     8161 ttagccaaag ctaatgtttt agttgatgat gttaatggta aagtatctac tattgatcct
     8221 ctttttgtag ctattgctga cttatcagaa tctgtatctg atttaaacct acaggcaaga
     8281 cacattggac aaaaggcaag tagtgcaacc tcaagtgtta ctaaggcagg gtcagcttta
     8341 gctattggaa aagcggcttc aaaaattttt cgtaaaaaag gagacaaaaa atgagtaaat
     8401 tatttaaaac tttagttatc agtgctgcat caggtgcagc agctgcttat tttttaacaa
     8461 caaaaaaagg aaaagagcta aggaaaaatg cagaaaaatt ctatggagaa tataaagaaa
     8521 atccagaaga atatcatcaa atagctaaag ataaagcaag tgaatattca aatttagctg
     8581 ttgatacttt taaagattat aaaggtaaat ttgaatcagg tgaattgaca acagaggata
     8641 tcgtctcagc cgttaaggaa aaaagcggag aagtagttga ctttgctaat gattttgtca
     8701 atcaagctaa atcaaaattc tcagacgagg atactgctaa aaaagaagat aaggctcctg
     8761 aaacaaaagt agaagatatt gtcattgatt ataaagaaaa cacagaagat aaagaaaaat
     8821 aaaaaagaag ctactataag tagcttcttt tttatgagat ttttattgtc tgagtgacca
     8881 aatgaatgaa gagactgcag atgtaactgc tagacttgcc agcatcgcta gtaagaaact
     8941 ccacacaggt gttaaactta gtgctagtaa taagaaagag attagcacag tggttatact
     9001 gaagagagcg atacgagcaa aaaagactag ctcacgcaac ttagcactgt gatcaaattc
     9061 ttgaaatact tgaaattttg gttgagcttg ctctgttttg ctttcgtgag tttgctcttg
     9121 cttaagtggt gatgacatag ctttctcctt tgatactagc atatcatatc acaaataata
     9181 gagtttgtca ctagtttttt gatactatga gttaaaataa aggttttttc tcaaaaaaac
     9241 aaaaacattg cccatattag tagtagaata ctaaaagtat tttttactac tttccttact
     9301 tttttaaatt ggaaaggaaa aaaatagata taatttcgta tagtatttca atactatgtt
     9361 tttttcttat ctgatagtgc ttaacctcta aagtgatata attttcgaaa agaatatttt
     9421 ttatttattc ttgtcaagaa atatgcttaa aaatgctgaa agtttgttat aatactaatt
     9481 atggaaaaaa taattttgac agcgacagct gaatctattg aacaagtaaa gcaattgcta
     9541 gcaataggaa ttgaccgcat ctacgtaggg gaagaaaatt acggtcttcg tttaccacat
     9601 tcattttcag acgatgaatt gcgtgaaatc gctaaattgg tacatgatgc tggtaaagaa
     9661 ttgacagtag cttgtaatgc actcatgcat caagaaatga tggataatat caaaccattt
     9721 ttggagttga tgaaagaaat taatgtggat tatttggtag ttggtgatgc aggagtattt
     9781 tatatcaaca agcgtgatgg ttataacttc aaacttatct acgacacttc tgtattcgtt
     9841 acatcaagtc gtcaagttaa tttttgggga caacatggag cagtagaaac ggttttagca
     9901 cgtgaaatac cttctgaaga actttttaag atgtctgaga accttgaatt tcctgctgag
     9961 attttagttt atggggcttc tgtaattcat cactctaagc gtccgttatt gcaaaactat
    10021 tataatttta ctcatatcac tgatgaaaaa acacgtgagc gtggtctttt tttagcagaa
    10081 ccaggtgatc cagaaagtca ctattctatt tacgaagata agcatgggac tcatattttt
    10141 attaacaatg atatcaatat gatgactaaa gtgactgaac tagttgaaca tcactttact
    10201 cactggaaat tagatggtat ttattgtcca ggagataatt ttgttgctat tgctgaaatt
    10261 tttgtcgaaa cagctcgttt gattgaaaat ggaacattca ctcaggatca ggctttcttg
    10321 tttgatgaac gaattagaaa actgcaccct aaaggacgtg gtttagatac tggattttat
    10381 gattttgacc catcaacagt aaaataagat ttatttgcta cgtgaaagtt ttttatctca
    10441 aagcgtagcc aattgcacta agaactgcat atcgattggt atgttaatta agatagaaaa
    10501 taattctttt atcttgaaaa tagaaggaaa tataagatgt ctaatgtaaa aaaacgccct
    10561 gaggttttat cacctgcagg aacactagaa aaattaaaag ttgctattga ttatggagca
    10621 gatgctgtat ttgttggagg tcaagcgtat ggtcttcgaa gtagagctgg taacttttct
    10681 atggaagagt tacaagaggg aataaactat gctcatgcga gagatgctaa agtttatgta
    10741 gcggctaata tggttactca tgaaggtaat gagcttgggg caggtccgtg gtttcgtgaa
    10801 ttacgtgata tgggactaga tgcagtcatt gtttcagatc ctgctcttat tgttatttgt
    10861 gctacagaag caccaggttt agaaattcat ttgtcaactc aagcctcttc cacgaactat
    10921 gaaacttttg aattttggaa agagatgggg cttactcgtg tcgtattagc acgtgaggtt
    10981 actatggcag agttggctga aatcaggaag aggacagatg ttgagataga agcatttgtt
    11041 catggcgcga tgtgtatttc atactcagga cgatgtgttc tatcaaacca tatgagccat
    11101 cgtgatgcta atcgtggcgg ttgctctcag tcatgtcgtt ggaaatatga cctctacgat
    11161 atgccatttg gacaagaacg tcaatcgtta aaaggcgaga ttccagaacc tttctcaatg
    11221 tcagctgtgg atatgtgtat gattgagcat attccagata tgattgaaaa tggcgtagat
    11281 agtttaaaaa tagaaggacg tatgaaatcc attcattatg tttctacagt aactaattgc
    11341 tataaagctg ctgtagatgc ctatatggaa agtccagaag cttttgaagc tattaaagaa
    11401 gacttgattg atgaactttg gaaggttgca caacgcgaat tagcaacagg tttttactac
    11461 catacaccaa ctgaaaacga acaactcttt ggagctcgtc gtaaaattcc tcaatacaaa
    11521 tttgttgggg aagtggtttc atttgacaat gctaaaatgg aggctacaat tcgtcagcgt
    11581 aatgttatta tggaaggaga tcgcatagaa ttctatggtc ctggcttccg tcactttgaa
    11641 tgttttattg aaggtctgcg cgatgctgaa ggaaataaaa tagaccgtgc tccaaatccg
    11701 atggaattat taaccataac attaccaaat ccagtaaaaa aaggggatat gattcgtgct
    11761 tgtaaagaag gattagtgaa cctttatcaa aatgatggta ctagcaagac tataagagct
    11821 tagatacttt tataactatt ttgtcatttt acatggtcaa tattactaaa aattgttaaa
    11881 atagaagaag ttcatttaaa tagaacaaaa agctataaaa ataacgaata gtataagtgg
    11941 aggtataaaa tgtcagaatt tacatttgaa attgttgaga agttattggt cctatctgaa
    12001 aatgaaaaag gttggacaaa agagttgaat cgtgttagtt tcaacggagc gccagctaaa
    12061 tttgatttgc gtacatggag tccagatcat acaaaaatgg gtaaaggtat cactttatct
    12121 aatgaagaat ttaaagtcat tttag
//
LOCUS       DATRQA010000055        12100 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.055, whole genome shotgun sequence.
ACCESSION   DATRQA010000055 DATRQA010000000
VERSION     DATRQA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12100
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.055"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(40..1467)
                     /locus_tag="VBD27_001104"
     CDS             complement(40..1467)
                     /locus_tag="VBD27_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO7025123.1"
                     /translation="MTIFPKHFLWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDF
                     TAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFKVFRTSIAWSRIFPNGDDSAPNEAGL
                     QFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGAIMADKSQLSPQELYQAIHHELVASARVTKLGRSI
                     NPNFKIGCMILAMPAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNN
                     GIEIKFEEGDEEVLAQNTVDFLSFSYYMSVTQAYDFENYQSGQGNILGGLTNPYLTTS
                     EWVWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVEDDYRIDYM
                     NQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRY
                     KKKSFGWYQKVIKTNGQSLFEHHNR"
     gene            complement(1484..3352)
                     /locus_tag="VBD27_001105"
     CDS             complement(1484..3352)
                     /locus_tag="VBD27_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEO7025124.1"
                     /translation="MTKYQETAKAILAAVGGEKNIQHVTHCVTRLRLVLDNDEIVNDQ
                     VIKTIPNVIGVMRKNDQYQIILGNDVNNYYNAFLALGHFENTTREFSSQKKSSIFEKL
                     IETIAGVIKPLIPALLGGGMLKVIGILLPMLGITSSSSQTVAFINFFGDAAYYFMPIM
                     IAYSAASRFKVTPVLAATVGGILLHPAFVTMVAEGKPLSFFGAPVTLASYGSSVIPIL
                     IMVFLMQYIERWINKIVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGKGLSSAML
                     SIYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVTTPDVLILPAMLASNLAQG
                     AASLAVAVKAKQKQTRQVAFAAGLSALLAGITEPALYGVTLKFKKPLYAAMISGGLVG
                     AYIGLVNIASYTFVVPSIIGLPQYINPQGGNNFSNAVIVAIATIILTFIITWFLGIDE
                     GENEKSSINAQEYTHIRSGLSKKETLYSPMVGNVLPLSKVPDETFSSKLLGEGLAITP
                     SVGEVYAPFDGEIISLFPTKHAIALKDDKGVEVLIHIGIDTVELNGEGFEQLVKVGDF
                     VKRGQLLLRMDIDFISSKGYSLISPVVVTNSIDQLEIIVKDEETMVTNEDDLLVIL"
     gene            complement(3345..4196)
                     /locus_tag="VBD27_001106"
     CDS             complement(3345..4196)
                     /locus_tag="VBD27_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO7025125.1"
                     /translation="MIIKRVLNHNAVISVTHQGLDVLLMGKGIAFKKRIGDRINSDAI
                     EKSFVLKNSDNMNRFTELFITVPEEVVACSERIINLGKIKLGKNLDEILYINLTDHIH
                     SAIERHEQRMVIQNPLRLEIQRYYPDEYSIGMKALELIKDELGICLTIDESAFIAMHF
                     VNAGLDNPFNEAHKITEIVSYIEQKVKIDFRTELDESSIDYYRFMTHTKLFAQRVLSG
                     MKYEDDDADLLLVVKKKYPREYKCVKEIGNNMAIQYQYQLNSSELLYLTVHVKRLVKN
                     LKEISDD"
     gene            complement(4534..5142)
                     /locus_tag="VBD27_001107"
     CDS             complement(4534..5142)
                     /locus_tag="VBD27_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEO7025126.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLEALLADVSQIPEDIRQAVINNGGGHLNHALFWELMSPEETQISQELSED
                     IDATFGSFEDFKAAFTAAATGRFGSGWAWLVVNAEGKLEVLSTANQDTPIMEGKKPIL
                     GLDVWEHAYYLNYRNVRPNYIKAFFEIINWNKVNELYQAAKA"
     gene            complement(5222..6259)
                     /locus_tag="VBD27_001108"
     CDS             complement(5222..6259)
                     /locus_tag="VBD27_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO7025127.1"
                     /translation="MIAIEEIGRITPDNLGLVTVLAGEDLGQYAQMKEKLFQVIGFNK
                     DDLAYSYFDLSEEEYQNAELDLESLPFLSDYKVVIFDQFQDITTDKKTYLDEQAMKRL
                     EAYLQNPVDTTRLVICAPGKLDGKRRLVKLLKRDARVLEANTLKESDLKTYFQKYAHQ
                     EGLVFEAGVFDELLIKSNYDFSDTLTNIAFLKSYKTDGHISSNDVREAIPKSLQDNIF
                     DLTQDVLLGRIDLARDLVRDLRLQGEDEIKLIAIMLGQFRMFLQVKILASKGKSESQI
                     VSELSHYIGRKINPYQVKFAVRDSRNLPLAFLKEAIRILIETDYAIKRGTYDKDYLFD
                     LALLKIAHKKP"
     gene            complement(6313..7041)
                     /locus_tag="VBD27_001109"
     CDS             complement(6313..7041)
                     /locus_tag="VBD27_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7025128.1"
                     /translation="MFHREDFSKALLKKALQPGMRVMDIGCGSGELTRLAADIVGKEG
                     DVVGIDINEQLLWQARHDNGYANVSYQNADLAALSSFETKSFDAIIGRRVLMYQKNPK
                     KVIESLTPLLKQNGFYVFQESDAMCLELAKPSFVNMNKIQSLIWQTVEKEGGHIHIGS
                     QLEQLMLEAGLIVDHFVSENVIQTSQTGTDLSWLLNIMKERIQKYSDLQGLEDVNLQK
                     ELEENNDHFIRDCQFGIISHRVDE"
     gene            7217..8209
                     /locus_tag="VBD27_001110"
     CDS             7217..8209
                     /locus_tag="VBD27_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7025129.1"
                     /translation="MKNYRKLIVLLLLIFFAIFMGAYAYTHIVEKRSLTSNTIEKTLP
                     VVNQIKPQTIKEYQNYLTKVAKRNVLPVDIPQALNNEKVEITATDGMQTFTWNDKNNP
                     KQKVIFYVHGGSYIHQASELQYIFVNKLAKKLDAKVVFPIYPKAPTYNYSDAIPKIKK
                     LYQNTLASVTSHKQIILVGESAGGGLALGIADNLVTEHIKQPKEIILISPWLDIATNN
                     PKIEKVQKKDPLLKAWQLQQVAPYWANGKKNFKNPQVSPLYSSQFNKMAPISFFIGTH
                     DIFYPDNQLLHQKLAKENIKHHYIVGQKMNHVYPVLPIPEAETAFKQMLKIINH"
     gene            complement(8268..9212)
                     /locus_tag="VBD27_001111"
     CDS             complement(8268..9212)
                     /locus_tag="VBD27_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7025130.1"
                     /translation="MTSISDIAKKAGVAKSTVSRVINHHPHVSDETRQKVMALITELD
                     YIPNQLARDLSRGKTQKIGVVIPHTRHPYFTQLINGLLDAAKTTDYQLVVMPSDYNQE
                     LELSYLKQLKMEAIDALIFTSRAISLDIIETYAKYGRIVVCEKLQEYNHLSSAYLDRY
                     SSFLEAFSDMKLRGLEHLVLLFSRNNESSATYQSALLAYQEVYGQLSSPYMVVGNVHD
                     FNDGLNLSYQLVKEVSIDGILATSDEVAAGLIKGYEESRKKCPYIIGQECLLVGQLLK
                     LPTIDHKSYYLGKLAFKQALAEKISQEVLFSEFLSQDN"
     gene            complement(9223..10032)
                     /locus_tag="VBD27_001112"
     CDS             complement(9223..10032)
                     /locus_tag="VBD27_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7025131.1"
                     /translation="MTIKRIFCDMDGTLLNSEGQVSKSNATLIREAAIPVTLVSARAP
                     MEMKDAVDALQLGGVQVAFNGGLIYRIGDNNQVLPIHTQIIKKSTVKQLLRGIRFHFP
                     QVSLSYYDLNNWYCDKIDEGIRYEHSLTQQCPTFIHNEDQFLEGHTNTFKIMMITFDE
                     ANMLELEKYLQSLELPEITIQRSGKAYLEITHLLAKKSKGIAYILQKEQLAREETAAF
                     GDGHNDLPMLEMVGYPIVIDNAFDDIKAIAYQLTKSNDEDGVGYGIQKFLK"
     gene            complement(10158..>12100)
                     /locus_tag="VBD27_001113"
     CDS             complement(10158..>12100)
                     /locus_tag="VBD27_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO7025132.1"
                     /translation="DVLSFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKL
                     EEAEERRHFNGFDYRQYLKRHGIYRIAKVTKIKQIRLFQHRSFFDLMFKWRRSAIVIS
                     QTFPNPMRHYMSGLLFGYLDKTFDDMSDLYSSLGIIHLFALSGMQVGFFLGIFRYICL
                     RIGLRLDHVWLLQIPFSLIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICL
                     ISLPHSLLTTGGVLSFAYAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYFSGF
                     QPISIILTALLSFAFDIIFLPLLTVIFVLSPFVKLSCINSLFEILEVLLKWTGQLFPR
                     PLIFGKPSLFLLIVMIIILGLLYDYYHSKCFRYCSLLIIFTLFFITKNPITNEVAILD
                     VGQGDSILVRDWLGKTILIDTGGRVRFEQPEEWKQKVNQSNAERTLIPYLKSRGISKI
                     DNLVITHTDTDHMGDMEVISKHFKVARLITSSGSLTNSQYVKRLSKIGVAVKSIEAGD
                     KLSVMGSYLQVLYPWHKGDGKNNDSIVLYGHLLGKGFLFTGDLEEEGEKQLLEAYPHL
                     SVDILKAGHHGSKGSSSLSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKSKIF
                     RTDQSGTIRLTGWWKWHIQTVR"
BASE COUNT         4029 a         2326 c         1788 g         3957 t
ORIGIN      
        1 tttatattaa attgaaacag agactagttg tttaacatgt tatctgttat gatgttcaaa
       61 caatgattgg ccatttgtct taatcacttt ttggtaccaa ccaaatgact tcttcttgta
      121 acgttgtaga ctccctgttc catcatcatt tcgatcaaca tatattagac cataacgttt
      181 acttagttga gctgtagaca tggatacaca atcaatgcat ccccaagatg tatatcccat
      241 tatttcaaca ccatcttcaa tagctttagc aacttgaact aaatgttgat tcatataatc
      301 aatacgataa tcatcttcga cagtataatc accgtctagt gtttctatca attgatcttt
      361 tgctcccaat ccattttcta caataaacaa cggaatctga taacgttcat aatactgatt
      421 taagactagg cgtagcccta tgggatcgat ttgccatacc cattctgaag ttgttaaata
      481 aggattagtc aggcctccaa ggatatttcc ctgaccactt tgataatttt caaagtcata
      541 agcttgtgtc acgctcatat agtaactaaa cgataggaaa tctactgtat tttgtgccaa
      601 tacttcctca tctccttctt caaatttaat ttcaatacca ttatttttga aataggactg
      661 aatataagtg ggatatttac ctctgacatg tatatctgaa aatagcagat tatgttgttc
      721 aaattgtctt gcagccagaa catctcttgg atcagatgtc ataggataag ctggcatagc
      781 cagaatcata cagccaattt taaaattagg gttgatagaa cgccctaatt ttgtcactct
      841 agccgatgct actaattcat gatgaatagc ttgataaagc tcctgaggac ttagttgtga
      901 tttgtcggcc ataatagcac cactggtgaa aggcatatgt aaaatagaat tcacttcatt
      961 aaaagttaac caatatttta ccttatcttt ataacgctcc atgactgttt gagcaaattt
     1021 ctcaaaaaat gctatcaaac gtctatcagc ccaaccatta taggtctttg caagatgaag
     1081 gggtgtttca taatgagata aggtaaccaa aggctcgata ttatatttaa gcaactcgtc
     1141 aaataaatta tcataaaatt gtaagccagc ttcgtttgga gcactatcat caccatttgg
     1201 aaaaattcga gaccaggcaa tagaggttct aaaaacttta aagcccatct ctgcaaacag
     1261 ttttatatca tttttatagt tatgataaaa atcaattgct tctagcttca aattgtctgg
     1321 agtaggtttg gcggtaaaat cgcctagacc accatttgga agtacatctt gtacagataa
     1381 acctttgcca tctgttctaa atgcaccctc tacctgattg gcagcaactg cacctcccca
     1441 tagaaaatgt ttaggaaaaa tcgtcatatt atcatccttc cttttaaaga attacaagta
     1501 aatcatcttc atttgtaacc attgtctctt cgtctttaac gataatttca agttggtcaa
     1561 ttgaatttgt aactacaact ggactaataa gagaataccc ctttgaacta ataaaatcaa
     1621 tatccattcg caaaagtaac tgtcctcgtt taacaaaatc gccaacttta accaattgtt
     1681 caaaaccttc tccatttagt tctacagtat ctattccaat atggattaac acttcaactc
     1741 ccttatcatc ctttaaggca atggcatgtt ttgtaggaaa taaacttata atttcaccat
     1801 caaatggtgc ataaacttca cccacactag gagtgattgc taaaccttca cctagtaatt
     1861 tagatgaaaa tgtttcatcg ggtactttag ataaaggtaa tacattgcct accataggtg
     1921 aatacaatgt ctcttttttt gataaaccac ttctaatatg tgtgtactct tgagcattaa
     1981 tactactttt ttcgttttct ccttcatcaa ttcccaagaa ccatgtaata atgaaagtca
     2041 aaataatagt agctattgct acaataacag cattactgaa attattgccc ccctgtggat
     2101 tgatgtattg tggcaaacca ataatagatg gcactacaaa tgtataggaa gcaatattaa
     2161 ctaatccaat ataagctcct actaaaccac ctgatatcat tgcagcgtat aaaggcttct
     2221 tgaatttcag tgtgacacca taaagtgcag gttctgtgat accagctagt agtgctgata
     2281 aacctgctgc aaaggccacc tgacgtgtct gtttttgttt agccttaact gcaacagcaa
     2341 gggaagctgc accttgagct aaattagaag ctaacattgc tggtaaaatc aaaacatctg
     2401 gtgtcgtaac agaagcagct aaaaagattg gagcaaatgc ccagtgcatt cctgtcataa
     2461 caaccaatgg cataatagca ccaagaatag ctaaagctaa ccatggtgcc acatggtaaa
     2521 tcgaaagcat tgcactagac aatcctttac caataattac tcccagtggc cccacgacca
     2581 ctaaggctaa aaagccagaa atgagaataa tcaaagtcgg ttgtaaaaag ctcttcatta
     2641 cactaggaac aattttatta atccatctct caatgtattg cataagaaaa accataatta
     2701 agattggaat aacagaagaa ccataactag ccagtgtaac cggagctcca aagaaagata
     2761 atggttttcc ttcagctacc attgtcacaa aagcagggtg taagagaatc cctcctactg
     2821 tagcagctaa tacaggtgta actttaaatc gtgacgcagc tgaataagca atcattattg
     2881 gcataaaata ataagcggca tcaccaaaaa aattgataaa agctactgtt tgagaactag
     2941 agcttgttat accgagcata gggagcaaaa tacctataac cttgagcata cctcctccta
     3001 gaagagcagg tataagcggc ttaatgactc ctgcaatcgt ttcaattaat ttctcaaata
     3061 ttgaactctt tttttgtgat gaaaattctc tggtagtatt ttcaaaatga cctaaggcca
     3121 aaaaagcatt ataataatta ttgacatcat ttcccaaaat aatttggtat tgatcatttt
     3181 tacgcataac accgataaca ttcggaatag ttttaataac ctgatcgttg acaatttcat
     3241 cattgtctaa aactaatcgt aatcgtgtca cacagtgggt tacgtgttga atattttttt
     3301 ctccccctac tgctgctaaa atagctttag cagtctcttg gtatttagtc atctgatatc
     3361 tcctttaaat ttttaactaa tcgtttaaca tgcaccgtca gatataggag ttcacttgaa
     3421 ttaagttgat attggtattg gatagccata ttgttgccga tctctttaac acatttatat
     3481 tctctcggat attttttctt aactaccaaa agtaaatcag catcatcatc ttcgtatttc
     3541 attcctgata aaacgcgttg agcaaataat ttggtatgtg tcatgaaacg ataataatca
     3601 attgaacttt catctagttc tgttcgaaaa tctattttta ctttctgttc aatgtaagat
     3661 acaatctcag tgattttatg cgcttcatta aacggattat ccaatccagc atttacaaag
     3721 tgcatagcaa taaaagctga ctcatcaatt gttaaacaaa tacccaattc atcctttata
     3781 agttccagag ccttcatccc aatggagtat tcatcaggat agtaacgctg gatttccaac
     3841 cttagtggat tttggataac cattctttgt tcatgtcttt caatagccga atgaatatga
     3901 tctgtcaaat tgatataaag aatttcatct aagttcttac ccaatttgat tttacctagg
     3961 ttgataattc gttctgaaca tgcaactact tcttctggaa cagtaataaa taactccgta
     4021 aaacggttca tgttatcact attttttaaa acaaatgact tctcaatagc atcgctatta
     4081 atcctatcac ctattctttt cttaaatgca attcctttcc ccatcagtaa cacatctaat
     4141 ccttgatgtg ttactgatat gactgcatta tgatttaata cgcgttttat aatcattaaa
     4201 gtcctagtcc ttcactcgca caataaaaaa tgacccatcc aagacacaac aaaaagtcgt
     4261 cgttgaatag gtccctgctc acaaaatgtg ataacatcct ttattatgca attttatctg
     4321 ttatcagctt gcttacctta aataataaca cctgaaaacg tcttaatttt atcatttctt
     4381 actaatcttg tcaagcgctt tcaataaact tattcgctta attgaagata ataggactgt
     4441 tctagttcta tctgagtttg gatttctaga cttttaaaaa cattatcaac ttttagaata
     4501 ataaaaaaac gagcctatcg actcgttttt ttattaagct ttagcagctt ggtaaagttc
     4561 atttacttta ttccagttaa taatttcaaa gaaagctttg atgtagttag gacgaacatt
     4621 acgatagttt aagtaataag catgctccca tacatcaagt cctaaaatag gtttcttacc
     4681 ttccataatt ggagtatctt gattggcagt tgaaagcact tcaagtttgc cttcagcatt
     4741 aacaacaagc caagcccaac ctgaaccaaa acgtcctgtt gctgctgctg tgaaagcagc
     4801 tttaaagtct tcaaatgaac caaaagttgc atcaatgtct tcagataact cttgtgaaat
     4861 ttgagtttct tctggtgaca tcaattccca gaaaagagcg tggttaagat gtccaccacc
     4921 gttattgatg actgcctgac gaatatcttc tggaatttga gaaacatcag ctaagagcgc
     4981 ctctaagtct tctccaattt caggatgttt ctcaagagca gcatttgcat tagcaacata
     5041 agttgcatgg tgcttatcat gatgtagtgt cattgtctca gcatcaatat gtggctcaag
     5101 cgcatcatat gcatagggta aatctggtaa aataattgcc atgtcgaaat cctcttttca
     5161 tctattgtta cccttattct ataggaattt ggaacagctt tcaactcttt tgtttcttta
     5221 attagggctt tttatgagca atttttaata aggctaaatc aaagagataa tccttatcat
     5281 aagtccctct tttaatagca tagtcagtct cgattaaaat cctaatcgct tcttttaaaa
     5341 aagcaagggg aaggtttcta gaatctctga cagcaaattt tacttgataa gggttgattt
     5401 tacgtcctat gtaatgggat agttcagaaa caatttgact ctctgacttt cctttactag
     5461 ccaatatttt aacttgcaaa aacatccgaa actgtcctaa catgattgca atcaatttga
     5521 tttcgtcttc gccttgtaga cgtaaatctc ttactaaatc acgcgctaaa tctatcctac
     5581 caagtaagac atcttgagtc aaatcaaata tattatcttg caggctttta gggatagcct
     5641 ctctaacatc attagacgaa atatgaccgt ctgttttata tgactttaag aaagcgatat
     5701 tagttaaagt atcactaaaa tcatagttag acttaatcaa taactcatca aaaacacctg
     5761 cttcaaaaac taatccttct tggtgagcat atttttgaaa atacgtcttt aaatctgatt
     5821 cttttaaagt attagcctct aaaacacgag catcacgttt caataacttt acaagtcgac
     5881 gttttccatc aagttttcct ggagcacaaa taacaagtct cgtagtatct acaggattct
     5941 gcaagtaggc ttccaagcgc ttcattgctt gttcatctaa ataagttttt ttatcagttg
     6001 taatatcttg aaactgatca aaaataacaa ccttgtagtc tgataaaaaa ggtaaacttt
     6061 ctaagtctaa ttccgcattt tgatattctt cttcacttaa atcaaaataa gaataggcta
     6121 aatcgtcttt attgaatcca attacctgaa atagcttctc tttcatctga gcatattgcc
     6181 ctaaatcttc tcctgctaaa acagttacta accctaaatt atctggggta attctcccaa
     6241 tttcttcaat cgcaatcata ttccttatta taccataagg aaacttttca aggtgtcaaa
     6301 taatacttaa aatcattcat caactctatg agaaataatt ccaaattgac aatctcggat
     6361 aaaatggtca ttattctctt ctaattcttt ttgtaaattg acatcttcta acccctgaag
     6421 gtcactatat ttttgtattc tttctttcat gatatttagt agccaagata gatctgtacc
     6481 agtttgtgac gtttgaatga cattttctga tacaaaatgg tcaacgatta agcctgcttc
     6541 taacattaat tgctcaagtt gacttccaat atgaatgtgt cctccttcct tctcaacagt
     6601 ttgccagatt agtgattgta ttttattcat atttacaaag ctaggcttag ctagttctaa
     6661 acacattgca tcactttctt gaaaaacata aaaaccattt tgctttaaca aaggtgttaa
     6721 actttctatt actttctttg gatttttttg atacattaat acacgacgtc caataatagc
     6781 atcaaaactt tttgtttcaa aggaggaaag tgctgctaaa tctgcatttt gataactaac
     6841 attagcatag ccattatcat gtctagcttg ccataaaagt tgctcattaa tatcaattcc
     6901 tacaacatca ccttctttac caacgatatc agcagccaaa cgcgttaatt ccccactacc
     6961 acaaccaata tccataactc tcataccggg ctgcaaagct ttttttaata atgcttttga
     7021 aaaatcttct ctgtgaaaca taataacact ctcctctctt ctattttagc aaattaaagg
     7081 ttcatacgta ctatcagcaa aaaattataa taggttgaaa ttttatagca ttattttctt
     7141 gttttcgtat ctactttatt atagaataac aactaatcat ttttagcatg acgagctcaa
     7201 ttacaaaagg ataatcatga aaaactatcg aaaacttatt gtactactac ttctaatctt
     7261 ttttgccatt tttatgggag catatgctta cacgcatatt gttgaaaaaa gatccctaac
     7321 tagcaatact attgaaaaaa ctctacctgt ggtaaatcag attaagcctc aaaccattaa
     7381 agaataccaa aattacttaa ctaaggtagc taaacgtaat gttcttcctg tagacattcc
     7441 tcaggcatta aataatgaaa aggtagaaat tactgctact gatggcatgc aaacattcac
     7501 ttggaatgat aaaaataatc ctaagcaaaa ggttatcttc tatgttcatg gaggatcata
     7561 tatccatcaa gcttccgaat tacaatatat ttttgtcaat aaactagcta aaaaattaga
     7621 tgcaaaagtt gtctttccta tttaccctaa agctcctaca tataattata gtgatgctat
     7681 ccccaaaatt aaaaaattat accaaaatac attagctagc gtcacatctc acaaacagat
     7741 tatcctagta ggtgaaagtg caggcggagg ccttgcttta ggtattgctg ataaccttgt
     7801 cacggagcat atcaaacaac caaaagaaat tattttaata tcaccatggt tagatattgc
     7861 tactaataat ccaaagattg aaaaagttca aaaaaaagat ccccttctta aagcttggca
     7921 attacaacaa gtagctcctt actgggcaaa tggaaaaaag aattttaaaa atcctcaagt
     7981 tagtcctctt tacagtagcc aatttaacaa gatggctcca atatcatttt ttataggaac
     8041 gcacgatatt ttctaccccg ataatcaatt attacatcaa aaattagcaa aagaaaacat
     8101 taaacaccac tatatcgttg gtcaaaaaat gaaccacgtt tatccagtct taccgattcc
     8161 tgaagcagag actgctttca aacaaatgct taaaattatc aatcattaaa taaaaaggtt
     8221 tccaatcaag tttagttgga aacctttttt ctattacttt tgaatgacta attatcttga
     8281 gataaaaact ctgagaataa aacttcttga ctaatctttt cagctaaagc ctgtttaaaa
     8341 gctaatttcc ctaaataata ggatttgtgg tcaattgttg ggagtttcaa aagttgccca
     8401 actaataaac attcctgacc aataatataa ggacattttt tcctactttc ttcataacct
     8461 tttattagcc ctgccgcaac ttcatcactt gtagctaata taccatctat actaacttct
     8521 tttaccaatt gataagataa atttagtcca tcattaaagt catgaacatt cccaactacc
     8581 atatatggag aggatagttg cccatagacc tcttgataag ctaataatgc ggattgatag
     8641 gtagcagagg attcattatt ccttgaaaat aataaaacta agtgctccaa acctcgtaac
     8701 ttcatatcag aaaatgcctc tagaaatgaa gaatagcgat caaggtaagc agaggataga
     8761 tgattatatt cctgcaattt ttcacaaact actattcgtc catattttgc ataagtttca
     8821 ataatgtcga ggctaattgc tcttgaagtg aatattaaag catcaatagc ttccatcttg
     8881 agctgtttca agtaggaaag ttctaattct tggttgtaat ccgaaggcat cacaacaagt
     8941 tgatagtcgg tagttttagc agcatctaat aggccattta ttaattgtgt aaaataagga
     9001 tggcgcgtgt ggggaatgac gacacctatt ttttgtgttt tgcctcgact taaatcccgc
     9061 gctagttgat ttggtatata atccaattcg gtgataagag ccattacttt ttgtcgcgtc
     9121 tcatcagaaa catgtgggtg atgattgata acacgggaaa ccgttgattt agcaacacct
     9181 gcctttttag ctatatcact aatacttgtc attataaggc tcctacttta aaaatttttg
     9241 aatcccatat cccactccgt cttcatcatt agacttagtt agttgatatg ctatcgcttt
     9301 gatatcatca aaagcattat ctataacaat agggtaacct accatttcaa gcataggcaa
     9361 atcattatgt ccatctccaa atgcagccgt ttcttctcta gctaactgct ctttttgaag
     9421 gatgtaagca attcctttag attttttggc taagagatgt gtaatttcaa gatacgcttt
     9481 acctgaacgt tgaatagtga tttctggtaa ctccaaactt tgaagatatt tttctaattc
     9541 tagcatattt gcttcatcga aagtaatcat cataatttta aaagtatttg tgtgaccttc
     9601 taaaaactga tcctcattat gaataaacgt tgggcattgt tgagttaaac tatgttcgta
     9661 tcggattcct tcatctattt tatcacaata ccagttatta agatcgtaat aagataaact
     9721 aacttgagga aaatggaacc gtattcctct tagcaattgt tttactgtgc tttttttaat
     9781 gatttgtgta tgaataggta gaacttgatt attatctcct attctataaa tcaatcctcc
     9841 attaaaagca acttggactc ctcctaattg aagtgcatct acggcatcct tcatttccat
     9901 aggtgcccta gcagacacta aagttactgg aatcgctgcc tcacgtatta aggtagcgtt
     9961 acttttactc acttgtcctt cactatttaa tagtgtacca tccatgtcac aaaaaatacg
    10021 tttgatagtc ataaaaaata cctctcttct tctgaaatca gtataaatgc tggaacccgt
    10081 tccatgtcaa gcttaaaatt actgaatcct tgcgaaaaat agattgtatt taacagatac
    10141 gcaatcattt taataaatca acgaactgtc tgaatatgcc acttccacca tcctgttagc
    10201 ctaattgtac ctgattgatc cgttcggaaa atcttgcttt taatcttttg gaacctttgt
    10261 aaagtctctt gatgaggatg ctggtaacga ttatttttac cagctgacac tagaaccaca
    10321 ctaggagaca actttttcag aaaggataga cttgatgagc ccttagaacc atgatgtcct
    10381 gctttaagga tatctactga taaatgagga taagcttcta ataactgctt ttctccctct
    10441 tcctccaaat caccggtaaa taagaagcct tttcctaata aatgtccata taaaacaatt
    10501 gaatcattat tttttccatc acccttgtgc catgggtaaa gtacttgtaa ataacttccc
    10561 atgacagaaa gtttatcacc agcttctata gattttacag ctactcctat ctttgataaa
    10621 cgcttaacgt actgcgaatt cgttaaagaa cctgaacttg taatcaaacg tgcaacttta
    10681 aaatgctttg agataacttc catatccccc atatgatctg tatcggtatg agttatcact
    10741 aaattatcta tcttgctaat acctctgctt ttcaagtaag gaatgagcgt tctctcagca
    10801 ttagactggt ttactttttg tttccattct tcaggctgtt caaatctcac ccttccccca
    10861 gtatcaatta aaattgtttt tcctaaccaa tccctcacta aaatactatc tccttgtcca
    10921 acatctaaaa tcgcaacctc gttagtaatt ggattcttag tgataaaaaa caaggtaaag
    10981 ataataagaa gggagcaata acgaaaacat ttagaatgat aataatcata aagtaatccc
    11041 aaaattataa tcatgactat taaaagaaaa aggctgggct ttccaaaaat aagtggtctt
    11101 ggaaacagtt gcccagtcca ttttaataat acttctagga tttcaaacaa actattaata
    11161 caacttaatt taacgaacgg tgataagaca aatataacag ttaataaagg caaaaatata
    11221 atatcaaatg caaaagataa aagtgctgtt aatataattg atattggttg aaaacccgaa
    11281 aaatagtagg ttagtatggg aagaattcct acagagactg tcaaagattc gatagctact
    11341 ttttttatac tcgaaaaatg atcaaaggag gtcatcgtaa gtataaaagc ataagcaaag
    11401 ctaagaactc ctcccgtagt caaaagtgag tgggggagag agataagaca aattaacaag
    11461 cagagagcaa agttctcatc tttcttgaca ccgctatgtg ataataaaga ttgaataagt
    11521 gccctaacga ctgagatact aaagcctgtt aaaccagcat aaattagcga gaatggtatt
    11581 tgaagtaacc aaacatggtc tagacgtaag ccaatacgta gacagatata acgaaaaata
    11641 ccgagaaaaa aacctacttg catacctgac aaagcaaata aatgtataat acctagacta
    11701 ctatataaat cggacatgtc atcaaaggtc ttatctagat atccaaacaa aagccctgac
    11761 atatagtgac gcataggatt tggaaaagtt tgactaataa caattgcact tctacgccac
    11821 ttaaacataa gatcaaagaa agacctatgt tgaaataagc gtatctgttt tatctttgtc
    11881 actttggcga tacgataaat tccatgtctt tttaaatact gacgataatc aaagccatta
    11941 aaatgccttc tctcctctgc ttcttcaagt ttgatatctg caattatcat tatattatta
    12001 tcaagatttt gaaaataatc tttctcactt ttatttttta atgtgtaaaa agcttgataa
    12061 gtgttacctg aagcatctgc ctggaatgat aaaacatcac
//
LOCUS       DATRQA010000056        11727 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.056, whole genome shotgun sequence.
ACCESSION   DATRQA010000056 DATRQA010000000
VERSION     DATRQA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11727
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.056"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            7..78
                     /locus_tag="VBD27_001114"
     tRNA            7..78
                     /locus_tag="VBD27_001114"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Gln,seq:ttg)
     gene            87..158
                     /locus_tag="VBD27_001115"
     tRNA            87..158
                     /locus_tag="VBD27_001115"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:120..122,aa:Glu,seq:ttc)
     gene            221..739
                     /gene="rimM"
                     /locus_tag="VBD27_001116"
     CDS             221..739
                     /gene="rimM"
                     /locus_tag="VBD27_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO7025133.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVTDFVEERFKKGQVLALFDEK
                     NQFVMDIEIASHRKQKNFDIIKFKGMYHINDIEKYKGFTLKVAEDQLSDLKDGEFYYH
                     EIIGLDVYEGEELIGKIKEILQPGANDVWVVERHGKRDLLLPYIPPVVLEVNLSNQRV
                     QVELMEGLDDED"
     gene            726..1481
                     /gene="trmD"
                     /locus_tag="VBD27_001117"
     CDS             726..1481
                     /gene="trmD"
                     /locus_tag="VBD27_001117"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO7025134.1"
                     /translation="MMKIDILTLFPEMFAPLEHSIVGKAKERGLLEINYHNFRENAEK
                     SRHVDDEPYGGGQGMLLRAQPIFDTIDKIDAQKARVILLDPAGRTFDQDFAEELSKED
                     ELIFICGHYEGYDERIKSLVTDEVSLGDFVLTGGELAAMTMVDATVRLIPEVIGKETS
                     HQDDSFSSGLLEYPQYTRPYDYLGMTVPDVLMSGHHENIRKWRLEQSLRKTLERRPDL
                     LENYAMTDEERLILEKIKTEIERTDTVNEQNNL"
     gene            1462..2454
                     /locus_tag="VBD27_001118"
     CDS             1462..2454
                     /locus_tag="VBD27_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7025135.1"
                     /translation="MNKTIYDITIVGGGPVGLFAAFYAGLRGVSVKIIESLSELGGQP
                     AILYPEKKIYDIPGYPVITGRELIDKHIEQLERFKDSIEICLKEEVLSFEKVDDVFTI
                     QTDKDQHLSRAIVFACGNGAFAPRLLGLENEENYADNNLFYNVTKLEQFAGKHVVICG
                     GGDSAVDWANELDKIAASVAIVHRRDAFRAHEHSVDILKASGVRILTPYVPIGLNGDS
                     QRVSSLVVQKVKGDEVIELPLDNLIVSFGFSTSNKNLRYWNLDYKRSSINVSSLFETT
                     QEGVYAIGDAANYPGKVELIATGYGEAPVAINQAINYIYPDRDNRVVHSTSLIK"
     gene            2591..3661
                     /locus_tag="VBD27_001119"
     CDS             2591..3661
                     /locus_tag="VBD27_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO7025136.1"
                     /translation="MTNTVTPKETAGSFINKVLGGTATAIVVALIPNAILATFLKPFL
                     SYGLAAEFLHIVQVFQFFTPIMAGFLIGQQFKFTPMQQLAVGGAAYIGSGAWAYTEVI
                     QKGVATGSFQLRGIGDLINMMLTAALAVLAVKWFGNKFGSLTIILLPIIIGTGVGYLG
                     WKLLPYVSYVTTLIGQGINSFTTLQPIAMSILIAMAFSMLIVSPISTVAIGLAIGLNG
                     MSASAASMGVASTTAVLVWATMKANKSGVPIAIALGAMKMMMPNFLKHPVMAIPMLMT
                     ATVSSLTVPLFKLVGTPASSGFGLVGAVGPIASFEAGASMLIVILSWLVIPFAVGFVS
                     HKICKDILKLYKDDIFVFEGQN"
     gene            3674..4597
                     /locus_tag="VBD27_001120"
     CDS             3674..4597
                     /locus_tag="VBD27_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO7025137.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNHDVILLDNWADHIMAIKENGL
                     KVTGDTEDLVKLPIMKPTDATEEADLIILFTKAMQLPNMLQDIKKIIGKKTKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLKGPGHAHLEGVGSVNLQSIDPNNQEAGHRV
                     TELLNEAKLQATYDENVLPNIWRKACVNGTMNSTCALLDCTIGQLFASEDGVNMVHEI
                     IHEFVTVGKAEGVELDEEEITKYVMDTSVKAAHHYPSMHQDLVQNQRLTEIDFLNGAV
                     NKKGENLGIDTPYCRLITQLIHTKENVLSIK"
     gene            4809..6443
                     /locus_tag="VBD27_001121"
     CDS             4809..6443
                     /locus_tag="VBD27_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEO7025138.1"
                     /translation="MMKKGQVNDTKQSYSLRKYKFGLASVILGSFIMVTSPVFADQTT
                     SVQVNNQTGTSVDANNSSNETSASSVITSNNDSVQASDKVVNSQNTATKDITTPLVET
                     KPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNVKWISY
                     KSFGGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEA
                     KVASPTQFTLDKGDRIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEK
                     VSPQPQARITKTGRLTISNETTTGFDILITNIKDDNGIAAVKVPVWTEQGGQDDIKWY
                     TAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGTNSSQEP
                     IENGLPKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSY
                     SGVRRYIPVKKLTTSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEF
                     NFQKGEKIHYDQVLVVDGHQWISYKSYSGIRRYIEI"
     gene            6587..7672
                     /locus_tag="VBD27_001122"
     CDS             6587..7672
                     /locus_tag="VBD27_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7025139.1"
                     /translation="MTLRELTIEEFKEHSGNYDSQSFLQTPEMAKLLEKRGYDVRYLG
                     YQVENKLEIISLSYIMPVTGGFQMKIDSGPVHSNSKYLKQFYKALQGYAKSNGVLELI
                     VEPFDDYQLFTSSGVPSNQGNDNLIEDFTSSGYHHDGLTTGFTGKYLSWHYVKNLEGV
                     TSETLLSSFSKTGRALVKKAMSFGIKVRVLKRDELHLFKEITTSTSNRRDYMDKSLDY
                     YQDFYDSFEGKAEFVIATLNFREYDHNLQIKAEALENKLKLLDERFRENADSPKYHRQ
                     RSEIINQLASFETRRQEVQSFIQKYDNQDVVLAGSLFVYSLKETVYFFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRGSTFYNLLGI"
     gene            7697..7822
                     /locus_tag="VBD27_001123"
     CDS             7697..7822
                     /locus_tag="VBD27_001123"
                     /inference="COORDINATES: protein motif:HMM:NF014445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEO7025140.1"
                     /translation="MLRFKQNFNGYIIRKMGTFNYYPSPFKYKGIQLLKKVLKRN"
     gene            7887..8696
                     /locus_tag="VBD27_001124"
     CDS             7887..8696
                     /locus_tag="VBD27_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7025141.1"
                     /translation="MLDIERKRDIIKLQTISIQGGTMLKSKRKEIILSRLEQNKSVTL
                     DELTSILETSESTVRRDLDELESEGFLKRVHGGAELPYSLGQELSNQEKSIKNVQKKL
                     DIARQTAKLIAKQDVIFIDAGTTTELLIDFLPHEQLTVVTNSIHHAAKLVDRGIKTII
                     IGGAVKHSTDASIGQVAINQIRQITVDKAFLGMNGIDEVYLTTPDLEEAAIKEAIINN
                     SQQTFILMDSSKIGQVTFAKVKEINDINLVTNKTDSELMTIIKEKMKVIQV"
     gene            8693..9604
                     /gene="pfkB"
                     /locus_tag="VBD27_001125"
     CDS             8693..9604
                     /gene="pfkB"
                     /locus_tag="VBD27_001125"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEO7025142.1"
                     /translation="MIYTVTLNPSIDFIVRLDTLLLGSVNRMTSDDKYVGGKGINVSR
                     ILKRLKIDNTATGFIGGFTGHFVEDGLVSEGIKTDFVSVNEDTRINVKVKAKIETEIN
                     GGGPRITNEQLHRLEKLLSRLTPEDTVVFAGSAPASLGNKVYNTLIPIAKKTGAEVVC
                     DFEGQTLLDALAYQPLLVKPNNHELADIFGVELEGLPDIEKYAHKILDKGAKNVIVSM
                     AGDGALLVTPEASYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSKNPVEALKWGVACG
                     TATTFSDDLATAEFIQDIYNKVEVEKL"
     gene            9601..11565
                     /locus_tag="VBD27_001126"
     CDS             9601..11565
                     /locus_tag="VBD27_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7025143.1"
                     /translation="MKIQDLLKKEVMIMDLKATSKEAAIDEMITKLVDTGVVTNFAIF
                     KDGIMKREAQTSTGLGDGIAMPHSKNAAVKEATVLFAKSASGVDYEALDGQPTDLFFM
                     IAAPDGANDTHLAALAELSKYLLKEGFADQLRQAKTPDDIIATFDSNSISQETVAPQT
                     VQSTSKGSDYIVAVTACTTGIAHTYMAEEALKKKAAEMGVGIKVETNGASGVGNKLTS
                     SDIARAKGVIIAADKAVEMDRFDGKPLVSRPVADGIKKSEDLINIILDNKAQTYHAKN
                     QNDKQSGESDGKSGLGSAFYKHLMGGVSQMLPFVIGGGIMIAIAFLFDNILGVPKDQL
                     SNLGSYHEIAALFKNIGGAAFAFMLPVLAGYIAYSIAEKPGLVAGFVAGSIASSGLAF
                     GKVPFAEGGKATLALAGVPSGFLGALVGGFLAGGVILLLRKLLSGLPKSLEGIKSILL
                     YPLLGVLITGFLMLLVNIPMAAINTALNTFLQGLSGSSAVLMGLLVGGMMAVDMGGPV
                     NKAAYVFGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFNNEERQSGL
                     TNIVMGLSFITEGAIPFGAADPARAIPSFIVGSALTGALVGLAGIKLMAPHGGIFVIA
                     LTSNPLLYILFILIGAIVSGVLFGLFRKTK"
BASE COUNT         3896 a         1752 c         2403 g         3676 t
ORIGIN      
        1 gtcttttggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
       61 cgaatccagc taccccagtt tcacatggtc cgttggtcaa ggggttaaga caccgccttt
      121 tcacggcggt aacacgggtt cgaatcccgt acggactatc gtggccagtt gcattttagc
      181 ggctggtttt tcttttattt tcaaaaatat cggagttact atggaatact ttaacgttgg
      241 aaaaattgtc aatacacaag gattacaagg cgaaatgcgt gtattaagcg tgactgactt
      301 tgtagaagaa cgctttaaaa aaggtcaagt gttagcttta ttcgatgaaa agaatcagtt
      361 tgttatggat attgaaattg ctagtcatcg taagcagaag aattttgaca ttataaaatt
      421 caaaggaatg tatcatatta atgatattga aaaatataaa ggtttcacac tgaaggtggc
      481 agaggatcaa ctgagcgacc ttaaagatgg tgaattttat tatcatgaaa ttattggctt
      541 agatgtctat gaaggggaag agctgattgg aaaaatcaaa gaaattttac aaccaggagc
      601 aaatgacgtt tgggttgtgg aacgtcatgg aaaacgtgat cttttattac cctatatccc
      661 gccagtagta ttagaagtga acctttctaa tcagcgtgtt caagttgaat tgatggaagg
      721 tttagatgat gaagattgat attttaactt tatttcctga aatgtttgcc cctctggaac
      781 attctatagt tggtaaggca aaagaaagag gacttcttga aattaattat cacaatttca
      841 gagaaaatgc tgagaagtct cgacatgttg atgatgagcc ttacggtggg gggcaaggga
      901 tgcttttacg tgctcaacct atttttgata caattgataa aatagatgct caaaaagcac
      961 gtgttatttt gctagaccca gcgggcagaa catttgatca ggattttgca gaagaactat
     1021 ccaaagaaga tgaattaatt tttatttgtg gtcactatga aggttatgat gaacgtataa
     1081 aatcattagt aacagacgaa gtatctttgg gagattttgt tcttacggga ggagaattgg
     1141 cggctatgac aatggtcgat gctaccgttc gtctgattcc agaagttata ggtaaagaaa
     1201 caagtcatca ggatgattca ttttcatcgg gcctattaga atatcctcag tatacgcgcc
     1261 catatgatta tttaggcatg acagttcctg atgttctcat gagtggtcat catgaaaata
     1321 tccgaaaatg gcgtttagaa caaagtttgc gaaaaacact agaacgtcga ccagacttgt
     1381 tagaaaatta tgcaatgacg gatgaagaga gacttatctt agagaaaatt aaaacagaaa
     1441 tagaaagaac agatacagta aatgaacaaa acaatttatg atatcactat tgtaggaggc
     1501 ggaccagtag gtctctttgc tgctttttat gctggactac gcggagtatc cgttaaaatt
     1561 attgaaagcc tttcggaatt aggaggccag ccagctatcc tttatcctga gaaaaaaatc
     1621 tatgatattc ctggttatcc ggtgattact ggacgtgaat taattgataa acatattgaa
     1681 caattagaga gatttaaaga ctctattgaa atctgtttaa aagaagaagt tttatcattt
     1741 gaaaaagtag atgatgtttt cacaattcaa acagataaag atcaacattt gtcacgtgct
     1801 attgtttttg cttgtggcaa cggtgctttt gctccgcgtt tactaggttt ggaaaatgaa
     1861 gaaaattatg cagataataa tttattctat aacgttacaa agttagaaca gtttgcaggt
     1921 aaacatgttg ttatttgtgg tggaggcgac tcagctgttg attgggccaa tgaattagat
     1981 aagattgcag ctagtgtagc gattgttcat cgtagagatg cttttagagc gcatgagcat
     2041 agtgtggata tactaaaggc atcaggtgtt agaattttaa ccccttatgt cccaattggt
     2101 ttaaatggag atagtcagag agttagcagc ttggtcgttc aaaaagttaa aggtgatgaa
     2161 gtaattgaat tacctttaga caacttgatt gttagttttg gcttttctac ttctaataaa
     2221 aatttacgtt attggaattt ggattataaa cggtctagta tcaatgtttc ttcacttttt
     2281 gagacgactc aagaaggcgt gtatgctatt ggagatgctg ctaattatcc aggaaaggta
     2341 gaattgattg caacaggata tggggaagca ccagtcgcta taaaccaagc tatcaattat
     2401 atttatcctg atcgagataa ccgtgtagtc cattcaacat cattgataaa ataactataa
     2461 ataaaactga taagtttata aaaaaggatt atatatattg ttaaaaaaat aacaaaactt
     2521 cctatttatt ttgtgatata attaactttg tcgttatgaa tgatattaaa taaatgaagc
     2581 gaggtatttt atgacaaata cggtaacacc aaaagagacg gctggctctt ttatcaataa
     2641 agtgttaggt gggacagcaa ctgcaatcgt tgtagcactc atcccaaatg ctatcctagc
     2701 cacttttttg aaaccatttt tatcttacgg acttgctgca gagttcttac acatcgtgca
     2761 ggtatttcaa tttttcacac caattatggc aggatttttg attggtcaac agtttaaatt
     2821 caccccaatg caacagcttg ctgttggagg tgctgcatat attggttctg gtgcgtgggc
     2881 ttacacagaa gttattcaaa aaggagttgc tactggtagt ttccaattgc gtgggattgg
     2941 tgatctcatc aatatgatgc taacagcagc tttagcagtt ttagcagtta agtggtttgg
     3001 taataaattt ggttccctaa ccattatctt gttacctatt atcataggta caggcgtagg
     3061 ctatttagga tggaaattac tcccttacgt ttcatatgtg acaacactta ttggtcaagg
     3121 aattaattct tttactactt tacaaccaat tgcgatgtca attctaattg cgatggcttt
     3181 ttcaatgctt attgtaagtc ctatttcaac agtagcgatt ggtctagcga ttggtcttaa
     3241 tggtatgtca gcaagtgcag cttcaatggg agttgcttca acaacagcag tgctagtttg
     3301 ggcaacgatg aaggctaata agtctggagt tccaattgct attgctttag gtgcaatgaa
     3361 gatgatgatg ccaaatttcc taaagcatcc agtaatggcg attccaatgc ttatgactgc
     3421 tacagtaagt tctctaacag tcccattgtt taaattggtg gggacaccag cttcgtctgg
     3481 ttttgggctt gttggtgcag taggtccgat tgcttcattt gaagcaggag caagtatgtt
     3541 gattgttatc ttatcttggc tcgttattcc ttttgcagta ggttttgtat cacacaaaat
     3601 ttgtaaagac attctgaaac tttataaaga cgatattttc gtttttgaag gtcaaaatta
     3661 atttggaggt tgtatgttag tttatattgc tggttcaggc gctatgggat gtcgttttgg
     3721 atatcaaatt tctaaaacaa atcatgatgt tattttactt gataattggg cagaccatat
     3781 tatggctatt aaagaaaatg gcttgaaggt aacaggtgat acagaggacc ttgttaaact
     3841 tcctatcatg aagccaactg atgcgacaga agaagcagat ttaattattt tgtttactaa
     3901 agcaatgcag ttgccaaata tgcttcaaga tattaaaaaa attattggta agaaaacaaa
     3961 ggtcttatgt cttttaaatg gattaggaca tgaagatgtc attcgtcaat atatccctga
     4021 acacaatatt ttgatgggag taacagtttg gactgcaggg ttgaaaggac cagggcatgc
     4081 tcatctggaa ggagttggtt ctgtaaatct tcaaagtatc gatcctaaca atcaagaagc
     4141 aggtcatcgc gttactgaat tgcttaatga agcaaaatta caggctactt atgacgaaaa
     4201 tgttttgcca aatatttgga gaaaagcttg tgttaacgga acgatgaatt caacttgcgc
     4261 cttgctagat tgtacaattg gtcaactctt tgctagtgaa gacggcgtaa atatggtaca
     4321 tgaaattatc catgaatttg ttactgttgg aaaagcagaa ggtgtagagc ttgatgaaga
     4381 agaaataaca aagtacgtta tggatacatc tgttaaagca gcccatcatt acccttcaat
     4441 gcatcaagac ttagttcaaa atcagcgctt aactgaaatt gatttcttaa atggcgcagt
     4501 gaataaaaaa ggtgaaaacc taggcattga tacaccttac tgtcgattaa tcacacaatt
     4561 aattcacact aaagaaaatg tacttagcat aaaataataa tcaaccggga gaatagtgcc
     4621 cggttttttg atatgcttaa tgataatgat tttgttatac taagaataat aagagttggg
     4681 tatttttttc gagaagtatt tacagtataa aatattacat ttgtattaca aatatttcta
     4741 aaatatgttg acatgttaca aaaaggatat tataatagta gtgtaaataa ataaggaagt
     4801 aggtgtttat gatgaaaaaa ggacaagtaa atgatactaa gcaatcttac tctctacgta
     4861 aatataaatt tggtttagca tcagtaattt tagggtcatt cataatggtc acaagtcctg
     4921 tttttgcgga tcaaactaca tcggttcaag ttaataatca gacaggcact agtgtggatg
     4981 ctaataattc ttccaatgag acaagtgcgt caagtgtgat tacttccaat aatgatagtg
     5041 ttcaagcgtc tgataaagtt gtaaatagtc aaaatacggc aacaaaggac attactactc
     5101 ctttagtaga gacaaagcca atggtggaaa aaacattacc tgaacaaggg aattatgttt
     5161 atagcaaaga aaccgaggtg aaaaatacac cttcaaaatc agccccagta gctttctatg
     5221 caaagaaagg tgataaagtt ttctatgacc aagtatttaa taaagataat gtgaaatgga
     5281 tttcatataa gtcttttggt ggcgtacgtc gatacgcagc tattgagtca ctagatccat
     5341 caggaggttc agagactaaa gcacctactc ctgtaacaaa ttcaggaagc aataatcaag
     5401 agaaaatagc aacgcaagga aattatacat tttcacataa agtagaagta aaaaatgaag
     5461 ctaaggtagc gagtccaact caatttacat tggacaaagg agacagaatt ttttacgacc
     5521 aaatactaac tattgaagga aatcagtggt tatcttataa atcattcaat ggtgttcgtc
     5581 gttttgtttt gctaggtaaa gcatcttcag tagaaaaaac tgaagataaa gaaaaagtgt
     5641 ctcctcaacc acaagcccgt attactaaaa ctggtagact gactatttct aacgaaacaa
     5701 ctacaggttt tgatatttta attacgaata ttaaagatga taacggtatc gctgctgtta
     5761 aggtaccggt ttggactgaa caaggagggc aagatgatat taaatggtat acagctgtaa
     5821 ctactgggga tggcaactac aaagtagctg tatcatttgc tgaccataag aatgagaagg
     5881 gtctttataa tattcattta tactaccaag aagctagtgg gacacttgta ggtgtaacag
     5941 gaactaaagt gacagtagct ggaactaatt cttctcaaga acctattgaa aatggtttac
     6001 caaagactgg tgtttataat attatcggaa gtactgaagt aaaaaatgaa gctaaaatat
     6061 caagtcagac ccaatttact ttagaaaaag gtgacaaaat aaattatgat caagtattga
     6121 cagcagatgg ttaccagtgg atttcttaca aatcttatag tggtgttcgt cgctatattc
     6181 ctgtgaaaaa gctaactaca agtagtgaaa aagcgaaaga tgaggcgact aaaccgacta
     6241 gttatcccaa cttacctaaa acaggtacct atacatttac taaaactgta gatgtgaaaa
     6301 gtcaacctaa agtatcaagt ccagtggaat ttaattttca aaagggtgaa aaaatacatt
     6361 atgatcaagt gttagtagta gatggtcatc agtggatttc atacaagagt tattccggta
     6421 ttcgtcgcta tattgaaatt taaagctaaa tctttttaga tttagcttta ctaatattat
     6481 gtgtgttgga aaaaacagtg gagatttacg tcaaaattat attatttgga tagtatcgaa
     6541 tagatagaat gtggagaaaa gtaggaaata gttaagaaag gtatttatga cgcttcgaga
     6601 attaacaata gaagaattta aagaacattc aggaaattat gattcacaat catttttaca
     6661 aacacctgag atggctaaac ttttagaaaa acgcggctat gatgttaggt atttgggata
     6721 tcaagtagaa aataaactag agataatcag tttatcttat attatgccag tcactggtgg
     6781 ttttcaaatg aaaattgatt caggaccagt tcattcaaat tctaagtatc taaaacaatt
     6841 ttataaagca ttgcaaggct atgccaaatc caacggtgtt ctagaattaa tagttgagcc
     6901 ttttgatgat taccaattat tcactagttc gggagttcct agtaatcagg gaaatgataa
     6961 tctgattgaa gattttacca gttcaggtta tcaccatgat ggtttaacaa ctggttttac
     7021 tggtaaatat ttatcttggc actatgttaa aaatttagaa ggtgtcactt ctgaaacgtt
     7081 actatcttca ttctctaaga caggacgagc tttggttaag aaagcaatgt cttttggaat
     7141 caaggttcgc gttcttaaac gtgatgagct acatttattt aaagagataa caacttctac
     7201 gtcaaataga cgtgattata tggataagtc cttagattat tatcaagatt tttacgatag
     7261 ctttgaaggc aaggctgaat ttgtgattgc cactttaaat tttagagaat acgaccataa
     7321 cttgcaaata aaagctgaag cattggaaaa taagcttaaa ttgcttgatg aacgttttag
     7381 ggaaaatgca gactctccta agtatcatag gcaacgctct gaaataatta atcaactggc
     7441 tagttttgaa acacgacgac aagaagttca gtcatttatt caaaagtatg ataaccaaga
     7501 cgttgtttta gctggtagtc tatttgttta tagtcttaag gagacagttt atttcttttc
     7561 aggatcctac acagaattta ataaatttta tgcaccagca gttcttcagg aatatgttat
     7621 gcaagaggct ctcaaaagag gttctacttt ctataacctc ctaggtattt agggaacttt
     7681 tgatggttca gatagtattc ttcgctttaa acagaatttt aatggttata tcattcgtaa
     7741 aatgggaact tttaactatt atccaagtcc ttttaaatac aaaggaattc agcttcttaa
     7801 aaaggttttg aagagaaatt aaagtatttg taaaagctaa tctttagatt aggctttttt
     7861 agtttattga tagtttttgg aagaaaatgc ttgacattga aagaaaaaga gatataatta
     7921 aattgcaaac gatttcaatt caaggaggga ctatgctaaa gtcaaagaga aaagaaatta
     7981 tcttatcacg acttgagcaa aataagagtg ttactttaga tgaattaacg tctatcttag
     8041 agacttctga atctacagtt cgcagggatt tagatgaact tgaatctgaa ggttttttaa
     8101 aaagggtgca tggtggtgct gaattaccat actcacttgg ccaggaatta agtaatcaag
     8161 aaaaatctat caaaaacgtt caaaaaaaat tggatattgc tagacaaact gcaaagctga
     8221 tagcaaagca agatgttata tttattgatg ccggaactac gacagaatta ttaattgatt
     8281 ttctccctca cgagcaatta acggtggtga cgaattctat tcatcatgct gcaaagttag
     8341 ttgatagagg gataaaaaca atcattatcg gaggagcggt taaacattca acagatgcta
     8401 gtattggcca ggtagccatt aatcagatta ggcagataac ggttgacaaa gcttttctgg
     8461 ggatgaatgg tatagatgaa gtttatttaa ctacaccaga tttagaagaa gctgctatta
     8521 aagaagctat tattaataat agtcaacaaa cctttattct aatggattca tctaaaattg
     8581 gacaagttac atttgctaaa gtgaaagaga taaatgacat taatttagta actaataaga
     8641 ctgattcaga acttatgaca ataataaaag agaaaatgaa ggtgattcaa gtatgattta
     8701 tacagtaaca ttaaatccat cgattgattt tattgttcgt ctagatacac tattgttggg
     8761 tagtgttaac cgtatgacta gcgatgataa atatgtaggt ggtaaaggaa ttaatgttag
     8821 tcgcatatta aaacgcttaa aaattgacaa tactgctaca ggttttatcg gtggatttac
     8881 aggacatttt gttgaagatg gacttgtatc agaaggtatc aaaactgact ttgtttctgt
     8941 taatgaagat acacgtatca acgttaaagt aaaagcaaaa attgaaactg aaatcaatgg
     9001 cgggggtcct aggataacaa atgaacaact tcatcgtcta gagaaacttc tttcgagact
     9061 aacacctgag gacacagtag ttttcgcagg ttcagcacca gctagccttg gaaataaggt
     9121 ctataataca ttgattccaa ttgctaaaaa gacaggagcg gaagttgttt gcgattttga
     9181 aggtcagaca cttttagacg ctcttgctta tcagcctctt ttagtaaaac cgaataacca
     9241 tgaattagca gatatttttg gcgtagagct agaaggtctg cctgatatcg aaaaatatgc
     9301 acataagatt ctagataaag gagctaagaa tgttatcgtt tctatggcag gggatggtgc
     9361 attgttagta actccagaag cttcatattt tgcaaaaccg attaagggtg aagtgaaaaa
     9421 ttccgttggg gctggtgatt caatggtagc tggctttaca ggagaatttg ttaagtcaaa
     9481 aaatccagta gaagcactaa aatggggcgt agcttgtggt acagcaacca ctttctcaga
     9541 tgatttagca acagctgagt ttatccaaga tatttataat aaagttgagg tagaaaaatt
     9601 atgaaaatcc aagacctact aaaaaaagaa gttatgatta tggatttaaa agcaaccagt
     9661 aaagaggctg caattgatga aatgattaca aaattagttg acacaggtgt tgtgacaaac
     9721 tttgctattt ttaaagatgg tatcatgaag cgtgaggcac aaacatcaac tggattaggt
     9781 gatggtatcg ctatgccaca tagtaaaaat gctgccgtta aagaagctac tgtccttttt
     9841 gcaaaatctg catcaggtgt tgattacgaa gcacttgatg gacagccaac tgatttattc
     9901 tttatgattg ctgcgccaga tggtgctaat gatacgcatt tagctgcctt ggcagaacta
     9961 tctaaatatc ttttaaaaga agggttcgct gatcaattac gtcaagcaaa aacgccagat
    10021 gacattattg ctacttttga tagtaatagc atttctcaag aaacagtagc tccacaaaca
    10081 gtacaatcaa caagtaaagg ttcagattat attgttgcag taacagcttg cactacggga
    10141 attgcccaca cgtatatggc agaagaagct ttaaagaaaa aagcagctga aatgggtgta
    10201 ggtattaaag ttgaaacaaa tggcgcttct ggtgtaggaa ataagttgac ttcaagtgat
    10261 attgcaaggg ctaagggtgt tattattgca gcagataagg ctgtagagat ggatcgtttt
    10321 gatggcaaac ctttggtatc acgtccggta gctgatggta ttaaaaaaag tgaagactta
    10381 attaacatta ttctcgataa taaggcacaa acctatcatg caaaaaatca aaatgataaa
    10441 caatcaggtg aatctgatgg aaaatcaggc ttaggctctg ctttttataa acaccttatg
    10501 ggtggtgtat cacaaatgtt accattcgtt attggtggtg gtattatgat tgcgattgct
    10561 ttcctttttg ataacatact tggtgtacca aaagaccaat taagtaattt aggttcatac
    10621 catgaaattg cagccttatt caaaaatatc ggtggcgcag catttgcatt tatgttacca
    10681 gtattggcag gttatatcgc ttattcaata gctgaaaaac caggactagt agctggcttc
    10741 gtagctggtt caattgcatc aagtggactg gcattcggta aagtaccttt tgcagaaggt
    10801 gggaaagcaa ccttagcatt agcaggtgtg ccatcaggtt tcttaggggc tttagtaggt
    10861 ggtttcttag ctggtggagt tatcctacta cttagaaaat tactttcagg tctcccaaaa
    10921 tcgcttgaag gtattaaatc aattcttcta tacccattgt taggtgtttt gattacaggt
    10981 ttcttgatgt tgctcgtaaa cattccaatg gcagctatca acacagctct taacactttc
    11041 ttacaaggtt tatctggtag ttcagcagtc ttgatgggct tactagttgg tggtatgatg
    11101 gctgtagata tgggtgggcc tgttaataaa gcagcttatg tttttggtac aggtactctt
    11161 gcagcaactg ttgcaaatgg cggctctgtt gttatggcag ctgtaatggc tggaggaatg
    11221 gttccaccgt tagcagtatt tgtggcaact ttattattta aagataaatt taataatgaa
    11281 gaacgtcaat ctggtctaac aaatatcgtt atggggttat cttttatcac tgaaggtgct
    11341 attccgtttg gtgctgctga cccagctcgt gcaatcccta gctttattgt aggttcagcc
    11401 ttaactggag cacttgttgg tttagctggt atcaagttga tggctcctca tggagggatt
    11461 tttgtcatcg cattaacaag taatccttta ctttatattc tatttattct aattggagcg
    11521 atagtctcag gtgtactctt tggcttgttc cgtaaaacaa aatagttaat ctcaacttat
    11581 ccaaatttac attgaataaa aggtgaataa actatcgtta ttgggtctat tcgccttttt
    11641 atgttgtaaa agaatggtag attaatcatt aataattgct aatctttttt caataggata
    11701 tgatttattt aagcaaaaaa atggata
//
LOCUS       DATRQA010000057        11489 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.057, whole genome shotgun sequence.
ACCESSION   DATRQA010000057 DATRQA010000000
VERSION     DATRQA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.057"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            199..810
                     /gene="rpsD"
                     /locus_tag="VBD27_001127"
     CDS             199..810
                     /gene="rpsD"
                     /locus_tag="VBD27_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO7025144.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKAKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(918..2000)
                     /locus_tag="VBD27_001128"
     CDS             complement(918..2000)
                     /locus_tag="VBD27_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7025145.1"
                     /translation="MERFIIVKINEVKKKDGTVIYRASVYLGTDKVTGKKVTTKITGR
                     TKKEVREKAKQGAVEFIKNGSTRFKATSVTSYQELATLWWDSYKHTVKYNTQLADKTN
                     KGERKAYLHYDRIHALNKRILQYGVIMQAIPFNPAREVILPRNTKKANTKRVKHFEND
                     ELRTFFNYLNNLDKSKYRNFYEVTLYKFLLATGCRINEALALNWSDIDLDNAVVHITK
                     TLNYKQEINSPKSKSSYRDIDIDSQTITMLKQYRRRQIQEAWKLGRSETVVFSDFIHK
                     YPNNRTLQTRLRTHFKRANVPNIGFHGFRHTHASLLLNTGIPYKELQYRLGHSTLSMT
                     MDIYSHLSKENAKKAVSFFETAINSI"
     gene            complement(3016..4173)
                     /locus_tag="VBD27_001129"
     CDS             complement(3016..4173)
                     /locus_tag="VBD27_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbhA"
                     /protein_id="HEO7025146.1"
                     /translation="MKKRIIVVLVALLAIGGGSAYYFLSYAPHQEAVTNFNKAVQTVK
                     SKNKTLKSEIAKAEKLVKSNQQPLDVRTLDNLKAAVATAKSDLREIPKVADKTEAIKK
                     QTKSLNQPIDYSKATQSLADSSLAYTNSVKQLAQITNPSQSFVEERLKEIDTVTGVQS
                     VTESNDPNGKLNKQGGYTASIYFTDNQVTEAVEGKDVLDKGTDAGGNIEVFKTKEEAE
                     KRNIYLSAFDSAGMFNPGSHYVFGTVVIRTSHYLTASQQKSLTQKIYEKLIEIKSKNS
                     GSTKDSKKKEVSKDNTKTSTQEQPAPSSTTDKSQESQSSSETSNSGGRGGTRGVEDSS
                     DTSSSSEMTEEELNSAIKEIQDSRDYYAPNEFENQVGDTTSSDNGDSTYHP"
     gene            complement(4428..5015)
                     /locus_tag="VBD27_001130"
     CDS             complement(4428..5015)
                     /locus_tag="VBD27_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7025147.1"
                     /translation="MNRLKELRANQKKTQQDMSEFLNMSRRGYQKIENGESQIKPDKA
                     QALADYFNVSVGYLLGYSDDKKDPMLTSFELARKDDTGEYFDLQDMVTLMDISMLKNV
                     DTRDKILSNLKEYYDYYGIRMKEEHEIKDSEKFNAFLAEFQEDTNDYIIMLLTGIVKL
                     RDDIRFTLLDILAMEGEKLETVKNVVKFISDNGDN"
     gene            5170..5355
                     /locus_tag="VBD27_001131"
     CDS             5170..5355
                     /locus_tag="VBD27_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025148.1"
                     /translation="MLITSTQAKAIRRKQADKQLTSKSASAEIGVTEVTYRKVRNGGE
                     VKNGIYQKVMEWLAEDY"
     gene            5371..5973
                     /locus_tag="VBD27_001132"
     CDS             5371..5973
                     /locus_tag="VBD27_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HEO7025149.1"
                     /translation="MRTETWNGYDIRFVEHQGEWWAVLADICHALDLKPKRVKERLVD
                     EVVSTDHVADSLGRQQEMLIVNEFGIYDTIFSSRKPEAKSFKFWVFETIKQLRQATGL
                     EGFEVFRMLDKEHQKEAMARLTNSLDRVSKKDLIKANTITNKAVSNKFGYSKMVKKSE
                     MTPDMLVAREMILDDTVELMGIKEKFGLNISVSESIYNKN"
     gene            6209..6847
                     /locus_tag="VBD27_001133"
     CDS             6209..6847
                     /locus_tag="VBD27_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HEO7025150.1"
                     /translation="MNIIKQVKSSFGELEIDFYLDRNRNIFVTIEQLAQGFGYKSRNA
                     IEKMIERQPYLKEKRFSVTDKLSATDGKQYETRLFNKRGIFEIGMLSKTEKGKIFRQW
                     IYDHIEELERENANFKLIRELEKSNHKELTQAIKDWEHFNQWSYKAISDLLLKSVTGQ
                     TAKQLKQSRVGYEIALDCLSADELTRYRKLEQKVIVLLELNAEYNDIKKLVL"
     gene            6946..7119
                     /locus_tag="VBD27_001134"
     CDS             6946..7119
                     /locus_tag="VBD27_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEO7025151.1"
                     /translation="MTDKRAVGRPPKGEQPNNIKITVRISQGLDEKVQAYADNHGLTK
                     PEAIRKALEDLSD"
     gene            7208..7714
                     /locus_tag="VBD27_001135"
     CDS             7208..7714
                     /locus_tag="VBD27_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025152.1"
                     /translation="MRTLFQSTQKTERECAFCVLKNQNNMIKKKRNPRVLFKNVAYKL
                     SEIEGKTLTEIASFLGFGNSEVCRSTLYNWKRRKWLTFDLKNGHYRNVEVLHEVTLEK
                     MANKELKEQGLIYKASIYYEQVVSTSEIIEDIKTKTQDRIKAIHLQQKALERIPSELF
                     AELYTNMN"
     gene            7791..8123
                     /locus_tag="VBD27_001136"
     CDS             7791..8123
                     /locus_tag="VBD27_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025153.1"
                     /translation="MKYRVETNPFSKDRYTPEQREMLKKRQLSKDKAEAYFARLYNHH
                     IARVIIANVMAEYTTTFRKSATTFEEAWGALGYKQTTEIVFRAVNGLPCSEKDTGELE
                     TYLSEVSA"
     gene            8120..8341
                     /locus_tag="VBD27_001137"
     CDS             8120..8341
                     /locus_tag="VBD27_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025154.1"
                     /translation="MRCIEELNEIANDILVDYEGLCGQLMDILNVLDLAAQEYSKETT
                     SAIINTSCRALKCLISDHDKMTHKYRKEL"
     gene            8344..8535
                     /locus_tag="VBD27_001138"
     CDS             8344..8535
                     /locus_tag="VBD27_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025155.1"
                     /translation="MQELNFTLTQTLILFIVLGLIGLLLSRSKPLIEIDLPEDTQAPK
                     PRQNANYGAYIQAQNHYYN"
     gene            8546..8874
                     /locus_tag="VBD27_001139"
                     /pseudo
     CDS             8546..8874
                     /locus_tag="VBD27_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8876..9148
                     /locus_tag="VBD27_001140"
     CDS             8876..9148
                     /locus_tag="VBD27_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025156.1"
                     /translation="MNVFSQDYEAKLLEQNLTAFNRFLEVYQQPKPRVLGLITAEQVK
                     EELNIKGKTLKRWENAGLKRYQPPLEDTRKHYYKVSDILIFLGVDV"
     gene            9149..10007
                     /locus_tag="VBD27_001141"
                     /pseudo
     CDS             9149..10007
                     /locus_tag="VBD27_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029301.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
     gene            10019..11413
                     /locus_tag="VBD27_001142"
     CDS             10019..11413
                     /locus_tag="VBD27_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HEO7025157.1"
                     /translation="MKDYQNKLSQATQPAFAPAFRTRKGRGDKEYVISSPYNVGKVFE
                     FYENIFTGIKYNEFEKTIEITKTLPWSKEKGLWTNEQTSLCIAFIDDKYRFTPRKEHI
                     EVAITALAKKNSYHPIKQRIESQKWDGKARGERYFIDLLGCADNSYNREIAKVWLTGL
                     IARIYLRKVKFEVVPILIDKRQGTGKSTVTKRLLPSYHTDSEIKFGKNDSDYQKIQAN
                     AIIELGELKGMSKAEIETVKSFISSDSDTYREPYERKATPHPRHCVFIGTANKKSFLK
                     DSGTERRFFPIECGINDVEKHPMEVEEDYFLQILAEAKVWFNNYEPLTPSKELMNQLA
                     DIQEDYKVEDVDKEIIDQLLNEFQIVEGWDSLSQYEQRQYVLKQLGEPLDNAQSYSDY
                     PSAQTNCLLQVTSPNHIAYLGFNQKPTQGGKALISQKIRDHLDNDDGWKKGENPARKR
                     LFKGGTPVSYYERV"
BASE COUNT         3824 a         2064 c         2126 g         3475 t
ORIGIN      
        1 aagagacata aaaacttgat tcaatattat tttcatgata taatatagct tgtgtgaaat
       61 aacagcagaa aaatatgaag ctcgtcaaca ggtggttaac taatctagta ccctttgctg
      121 tttaggcgaa aatcccctgc acgaatcagg attttttaaa tcgttatttc ctcaattatt
      181 ttagaggagg acatttagat gtcacgttat actggtccat catggaaaca atcacgtcgc
      241 cttggtttat cacttacagg cacaggtaaa gaattggcac gtcgtaacta cgtacctggt
      301 caacacggtc ctaataaccg tagcaagctt tcagaatatg gcttgcagtt agctgagaaa
      361 caaaaacttc gtttttcata cggacttggt gaaaaacaat tccgtaactt gttcgtacaa
      421 gctactaaag caaaagaagg aactcttggt ttcaacttta tggttctttt agagcgtcgt
      481 cttgataacg ttgtttatcg tttaggactt gcaacaactc gtcgccaagc tcgtcaattt
      541 gttaaccatg gtcatatcct tgttgatggt aaacgtgttg atatcccatc ataccgcgtt
      601 actccaggac aagttatttc agttcgtgaa aaatcaatga aagtacctgc aatccttgaa
      661 gctgttgaag ctactttagg acgtccagct ttcgtatcat ttgatgctga aaaacttgaa
      721 ggttcattga ctcgccttcc agaacgcgat gaaattaacc cagaaatcaa tgaagcactt
      781 gtcgttgaat tctacaacaa aatgctttaa ttttaagatt aaacttactg aaagcctaca
      841 acagtgggct ttttctctta tcttaaaaca ttgatttctg ggtttgctta cttttttgct
      901 aacttttgat ttttttgcta tattgagtta attgctgttt caaagaatga gacagctttt
      961 tttgcattct cttttgataa atggctataa atatccatag tcatagatag agtagagtgc
     1021 cctaacctat attggagttc tttgtagggg atacctgtgt tgagcaataa actagcgtga
     1081 gtgtgacgaa aaccatgaaa gcctatattc ggtacattcg ctcttttaaa atgtgttctt
     1141 aatcgagttt gtaaggttct attgttgggg tacttatgga taaaatcaga aaataccact
     1201 gtttctgaac gacctaactt ccatgcttct tgaatctgtc ttcgtctata ctgcttaagc
     1261 attgtaattg tttgactatc tatgtcaata tcacgatagc ttgactttga ctttggacta
     1321 ttaatttctt gcttgtaatt tagcgttttt gtgatatgaa caacggcatt atccaagtcg
     1381 atatctgacc agtttagagc taacgcttca ttaatgcgac aacctgtagc taacaaaaac
     1441 ttataaagtg tgacttcgta gaagtttcta tatttacttt tatctagatt gtttaagtag
     1501 ttgaaaaatg ttcttagctc atcattttca aaatgcttta ctcttttagt gttagctttc
     1561 ttagtgttgc gagggagaat gacctcacga gcagggttga atggtatagc ttgcatgata
     1621 acaccatatt gtagtatacg tttatttagc gcgtgtattc tgtcataatg gagataagct
     1681 tttctttctc ctttattagt tttatcagct agctgagtat tgtattttac ggtatgtttg
     1741 taactatccc accataaggt tgcaagttcc tgatagcttg ttacggaagt agctttgaag
     1801 cgagtagaac cattttttat aaactcaaca gctccttgct tagctttttc tctaacttct
     1861 ttcttagttc gtcctgttat tttagtggtt actttcttac ctgttacttt atcagtacca
     1921 agataaacac tggcacgata aattaccgta ccatctttct tttttacttc atttattttc
     1981 acgattataa acctttccat cagcaggcaa gctattatta aaagggtttt agattagttt
     2041 atatcatggt ataataacgg tatcgggtta atatccgaaa tattataaat agaaaattgt
     2101 gatataatat tgttgttttt taagaccttg cgagcgtgga aaacttgcga ggtctttttt
     2161 tgtttggttt tagtaagttg gtaaattttt agatagccca gaaaagctgg ctgtctcttg
     2221 gctattgtta gccgtatata tcaaaaattg taattcattc atggctattc ctttaaggtt
     2281 acccccatca ttaaaaaatg ttgttcaatc cgagtttgga aaccggtggg aagggtcaac
     2341 tgtccaaata ttcgcttttt ttctcacacg acccccctcc ccagggagaa aaactagaaa
     2401 ggagatgtga atttttggat gaattaaaac gtttagaata gcaatctcat cacatttata
     2461 agcaaaatta acacagacag tagcaaaaga acttccaaat tattcaacgt tgagtaattt
     2521 aggtagttca gccctctacc tcatcgctac ccttccagat gaccaaaagc aagaacagct
     2581 tgaataacct atggcttcct atggtggaag gctgacgctc ctgaatcgtt caacgttgag
     2641 agatttggca actccgttga catgttgctg tagtccctaa atgttggtac gtgccaacat
     2701 aatacgtcaa cctatttcaa tagtcctaaa acgctcggta aattcactta aagaacttaa
     2761 aaaaacttga tatatcggtc gtttattaat ttattttttc cgttgtgata gcttctaaga
     2821 tcagtagact tttttagcat atagtattca gaaatcggac ttttcggctc tcctaaaagg
     2881 ttgttaaaga taattatttg gtgaaaaatg atggaatttc gagtgtgaac agagagcgaa
     2941 ctccgagaaa ttggcttgaa gctagagcga agtaagagaa atatagtaaa cggtgtttca
     3001 tattgaggga ataactcatg gatgataagt actatctcca ttatcactgc tagttgtgtc
     3061 accaacctga ttttcaaatt cattcggtgc atagtaatct cttgagtctt ggatttcctt
     3121 aattgcagaa tttaattcct cttccgtcat ttcacttgat gaagatgtat cacttgaatc
     3181 ttccacaccc cgagtgccac ctctaccacc actatttgaa gtttcactgc ttgactgtga
     3241 ttcctgactt ttgtccgtag ttgatgaagg tgcaggctgt tcttgtgtac ttgtttttgt
     3301 attatctttg ctaacttctt tcttcttgga gtctttggta gaaccagaat tttttgattt
     3361 aatttctatc aatttttcat atattttttg tgttaaagat ttctgttggc ttgctgttag
     3421 atagtgagat gtcctaataa caacagttcc aaatacatag tgagaacctg gattaaacat
     3481 acctgcactg tcaaatgcac tcaggtatat attacgtttt tctgcttctt ctttggtttt
     3541 aaaaacctca atatttcctc ctgcatcggt ccccttatcg agcacatcct taccttctac
     3601 agcttcagtt acctggttat cagtaaagta gattgaggca gtataaccac cttgtttatt
     3661 tagttttcca ttagggtcat tgctttctgt cactgactga acgcctgtaa ctgtatcaat
     3721 ctcttttaag cgctcctcaa caaaactttg actgggattg gttatttggg ctagttgctt
     3781 aacactattt gtatatgcta aagaactatc agctaaagac tgagtagctt ttgaataatc
     3841 tattggttga ttaagcgact tggtttgttt cttgatagct tcggttttat ctgctacctt
     3901 tggaatttct cttaagtcac ttttggcagt tgctactgct gcttttaggt tgtctaaagt
     3961 ccttacatct agcggctgct ggtttgactt aacgagcttt tcagctttgg caatctcact
     4021 ttttagcgtc ttattctttg acttaactgt ctgaacagct ttattaaagt ttgtgacagc
     4081 ctcttggtga ggggcgtaac ttaggaaata ataagctgaa ccaccaccaa tagctagcaa
     4141 tgcgaccagc acaacaataa ttctcttttt catgtttgtt tctcctctat gcgcgtgagt
     4201 taatgcggaa aatcaatatc taaacccgct agtccctcta aaatatttat tatgctttcc
     4261 ctttcttttg gcgttagtaa agaaaaataa aaaataagct ttttaaaaac atttggggta
     4321 tccccaccgt caataaattc aataaatttc tttgtataaa gcgtttaaat acagatacca
     4381 tggttttatt tatatagatg aaacccttga aaatagtggc gcgtgggtta gttgtcacca
     4441 ttgtcagata taaatttgac aacattttta acggtttcta gtttctcacc ttccatagct
     4501 agaatatcca ataaggtgaa acgaatgtca tcacgaagtt taactatacc cgtaagcagc
     4561 atgatgatat agtcgtttgt atcttcttga aattctgcta aaaaagcatt gaatttctca
     4621 ctgtctttta tttcgtgttc ttctttcatt cgaataccat aataatcata atattctttt
     4681 aaatttgaaa gtatcttatc gcgagtatct acatttttca acatagatat atccataaga
     4741 gtaaccatgt cttgtaaatc aaaatactct cctgtatcat cttttcgagc taattcaaag
     4801 ctggttagca ttgggtcttt tttatcatcg ctatacccca acagataccc cacagacacg
     4861 ttaaaataat ccgctagggc ttgggctttg tctggtttga tttggctttc accattctct
     4921 attttctgat accctcggcg actcatattc aaaaactcag acatatcctg ctgagtcttt
     4981 ttttgattag ctcttaattc tttcaatcta ttcataattt attacctcaa gtcgattata
     5041 tcataaaaag aaaactaagc taactttttt tacacttttt agaatcacct tcttgacaac
     5101 gaattataag ttagctaaaa taaaacctgt taactaacaa aaagttagca aagaaaggag
     5161 ttgaaattat tgcttattac ctcaacacaa gcaaaagcta tccgaagaaa acaagctgat
     5221 aaacaattaa cttctaaatc agcaagcgct gaaattggag ttacagaagt gacttatcgc
     5281 aaagttcgta atggcggaga agtaaaaaat ggcatttatc aaaaagtcat ggaatggctt
     5341 gctgaagatt actaaagaaa ggacaattta ttgagaacag aaacatggaa tggatatgat
     5401 atccgatttg tagagcacca aggcgaatgg tgggcagttc ttgctgatat ttgtcacgcg
     5461 ctagacttaa aaccaaaacg tgtgaaagag cgtttagttg atgaggtcgt ttcaaccgac
     5521 cacgtcgcag acagtttagg acgtcaacaa gaaatgctaa ttgttaacga gtttggaatt
     5581 tatgacacta ttttttcaag tcgtaaacca gaagcaaaat catttaagtt ttgggtattt
     5641 gaaaccatta aacagttaag acaagcaact ggtctagaag gctttgaggt ctttagaatg
     5701 ctggataagg aacatcaaaa agaagctatg gcaagattga ctaatagctt agatagggta
     5761 tctaagaaag acttgattaa agctaacaca atcacaaata aagccgtttc caacaagttt
     5821 ggatattcaa aaatggttaa aaaatcagaa atgacaccag atatgttagt tgctagagaa
     5881 atgattttag atgatactgt ggaactcatg ggaattaaag aaaagttcgg attgaatatc
     5941 agtgtgtctg agtcaatcta caataaaaat taaaaaaagc gcaaaaatgc gcgtgaaaac
     6001 aacaaaaaaa ggcttaccga gaccaatcag cagagccttt aactagtata actaaactca
     6061 attaataaag caggcaagct attattaaag gggttttagt aaaagatttg atacttccat
     6121 tgtatcatac taccgcagag caggcaacga cttaacagtt gcagttctcc ccgacaaaac
     6181 aacaataatc aaataacgag gtaaaacagt gaatatcata aaacaagtaa aaagttcttt
     6241 tggagaactt gaaattgatt tttatctgga caggaataga aatatttttg tgacgattga
     6301 acaattagcg cagggatttg gatataagag ccgaaatgct attgaaaaga tgatagagcg
     6361 ccaaccctac ctcaaagaaa agcgattttc agttactgac aaattgtcag ctactgatgg
     6421 caaacaatac gagacccgac tattcaataa gcgaggaatt tttgagatcg gtatgctgtc
     6481 caaaacggag aaaggtaaaa tctttcgtca atggatttat gaccatatcg aagaactaga
     6541 aagagaaaac gctaacttta aactgatacg agagcttgaa aagtctaatc ataaagaatt
     6601 aacacaagct atcaaggatt gggaacactt taatcaatgg agctacaagg ctattagcga
     6661 ccttttgcta aaatctgtca caggacagac tgctaaacaa ctgaaacagt cacgggtagg
     6721 ttatgaaatt gcattagatt gcctaagtgc tgatgagttg acgcgatata gaaaacttga
     6781 acaaaaagta attgtcctat tggagctcaa tgcagaatat aacgatatta aaaaattagt
     6841 cctttaacaa ctgaatagag tacgcaaaaa agtttgattt aggggttgac ttattaagac
     6901 gacttaatta taataattat gttgtcaaaa ctaaggaggt atacaatgac tgacaaaaga
     6961 gcagtcggca gaccaccaaa gggcgagcaa ccgaacaata tcaagataac ggttagaatt
     7021 agccaaggac ttgatgaaaa ggtacaagca tatgctgata atcatggctt aacaaagcca
     7081 gaagcaatca gaaaagccct tgaagatttg tcagactaaa caaaaaaata gcacagtaac
     7141 cgccaacgcc aatttttggt tactatgcta tcgctcgacc cacaaagtga gtacgtaaat
     7201 attatacatg cgtactctat ttcagtcaac acaaaaaaca gaaagggagt gcgctttttg
     7261 tgtgctcaaa aatcaaaaca atatgattaa gaaaaaaaga aatccgagag tactatttaa
     7321 aaatgtcgct tataaactat ctgaaattga agggaaaaca ttaacagaaa tcgcttcttt
     7381 tcttggattt gggaattcag aagtttgcag aagtacccta tataattgga agcgtagaaa
     7441 atggctaaca ttcgacctta aaaacggtca ttatcgcaat gttgaagtat tacatgaagt
     7501 tactcttgaa aagatggcta acaaggaatt aaaagaacaa ggactcattt ataaagctag
     7561 catttactat gaacaagtgg tttcaacatc cgaaattata gaagacatta aaaccaaaac
     7621 acaagataga attaaagcta tccacttaca acaaaaagcg ctagagcgta tccctagcga
     7681 gttattcgca gaattataca ctaacatgaa ctaatcataa aactagcatg aatctagtat
     7741 aagaaacaag caattacaca gaaaataata attaagcgag aaaaaacata atgaaatata
     7801 gagtagaaac aaatcctttt tcaaaagata gatacactcc tgaacaacga gaaatgctca
     7861 aaaaacgcca actcagcaaa gataaagcgg aagcctattt tgctcgacta tataaccatc
     7921 atatcgctag ggtgattatt gctaatgtaa tggcagagta tacgactaca ttcaggaaaa
     7981 gtgccactac ttttgaagaa gcatggggcg ctttaggtta taaacaaacc acagagattg
     8041 tctttagagc cgttaacggt ttaccatgtt cagagaaaga cacaggggaa ttagaaactt
     8101 atttaagtga ggtatccgca tgaggtgtat tgaagaatta aatgaaatcg ctaacgacat
     8161 tttagtagat tatgaaggtc tttgcggtca acttatggat attctaaatg tcctagattt
     8221 ggctgctcaa gaatatagta aggaaacaac tagtgcaatt ataaacacga gttgtcgcgc
     8281 tttgaagtgc cttatttctg accatgataa gatgacacat aaatacagaa aggagttgta
     8341 accatgcaag aacttaactt tacactaaca cagacactta ttttattcat tgttttaggt
     8401 ctcatagggc ttcttctaag ccgttctaag ccattaatag agattgactt accagaagat
     8461 acccaagcac ctaaaccacg tcagaatgca aactatgggg cttatattca agcacagaac
     8521 cattattaca attaggggga actgaatgac attgcctgaa aattatagac gtgtccttaa
     8581 tctgattaag gttggggcag acaatcccat tacagggcag agattggctt aatactgaaa
     8641 cgcgaagaac gctccgtcca aagtatcatc agtagcttaa tcacgcgcta caacgtccct
     8701 attattggca ttagacacgg attcaatcgt ggttacttta tcccagctaa taaagaagaa
     8761 ttactagatg gtgctaaagc cttttataat caagtacaaa aggaacaaca acgcctaagt
     8821 gtcttattga atgccgattt aaccagttat aaggaattac tcaaaggagg ttagtatgaa
     8881 cgtatttagt caagattatg aagcaaaact cttagaacaa aatctgaccg cttttaatcg
     8941 cttcttagaa gtataccagc aacctaaacc aagagtttta gggttgatca cggctgaaca
     9001 ggtcaaagag gaattaaata tcaaaggtaa aaccctaaag cggtgggaaa acgctgggtt
     9061 aaaacgatac caaccaccac tagaagacac aaggaaacat tattacaagg tcagtgatat
     9121 tcttatcttt ttgggggtgg atgtgtagat ggctatttat gaagcaagag gctttagctc
     9181 ttatttgtac ccctacaaag gacaattaga accgtttgac tatatcgcac agtttaaacc
     9241 aatgaaaacg cctgaggcta tcgatattga agaatacaag cgaacacaag ctccgtactg
     9301 tttgagtggc aaggtaaccc cagagaaaaa cggtagctat aagcgcaata atgctagtct
     9361 agtctatcgt gatttgattt ttcttgacta tgacgagata gaaacagacg taaacctgcc
     9421 taaaatcgtt tctaagacac tttgggaata cagctatatt attttatcca acgattaaac
     9481 acacccctga gaagccacgc tatcgccttg tcgtgaagcc tagtgacact atggacaaaa
     9541 cgacgtataa acaagtggtc aaggagatag ctaataagat tggactaccg tttgatttag
     9601 ctagtcttac ctggtcgcaa ttacaaggct tacccgtcac aacaggcgac ccagaagact
     9661 atcagcgcta tgtgaaccgt ggtcttgatt atcctgtccc taaagcaccc aaccgacagg
     9721 ttactactac ttacacgtca cgccctagaa gtcagcgttc tattaccatg agggtcatag
     9781 ataccttgtt taatggtttt ggagacgaag gcggacgtaa tgtagcctta actaaatttg
     9841 ttggtttgct atttaataaa tgggtggatt gtgatttaga gacggcttat gagctggtac
     9901 aaatagctaa cagcgtgaca actaagccac tacccattga tgagatagac aaaactttta
     9961 gaagcatact cgatacagaa ctaagaaaga gaggaatcaa gccatagaaa aggaagaatt
    10021 gaaagattat caaaataaac tatcacaagc cactcaacct gcttttgccc ctgcattcag
    10081 gacgcgaaaa ggtagaggag acaaagaata tgtcattagt agcccctaca atgtcggtaa
    10141 ggtttttgaa ttctacgaaa atatcttcac aggtattaaa tacaacgaat ttgaaaaaac
    10201 tattgaaatc actaaaacac tcccttggtc taaagaaaaa gggctatgga cgaatgaaca
    10261 gaccagcctt tgtattgcat tcattgatga taaatatcgg tttacccctc gtaaagaaca
    10321 tatagaggta gctattaccg ctttagctaa aaagaactct taccacccca ttaaacagcg
    10381 tattgaaagt caaaaatggg atggtaaagc tagaggagaa cgctacttta ttgatctatt
    10441 aggctgtgct gataattcct ataatagaga aattgccaaa gtatggctaa caggtcttat
    10501 agctagaatt tatctccgta aagtaaagtt tgaagtcgtt cctattctca ttgataaaag
    10561 acaaggaact ggaaaaagca ctgttactaa gcgcttgctt cctagctacc acaccgattc
    10621 agaaatcaag tttggtaaaa atgatagtga ttatcagaag atacaagcca atgccatcat
    10681 cgagctaggg gagctaaaag gtatgtcaaa ggcagaaatt gaaacggtta aaagcttcat
    10741 ttcctcagat agtgatactt atcgtgaacc ttatgaacgt aaagccactc ctcatccaag
    10801 gcactgtgtc tttatcggga cagctaacaa aaaatctttt cttaaggata gtggaacaga
    10861 aagacgcttc ttccctattg aatgtggtat caatgacgtg gaaaaacatc ctatggaggt
    10921 ggaagaagat tatttcttac agatactcgc tgaagccaaa gtatggttta acaattatga
    10981 accactaaca ccatctaaag agttgatgaa tcagttagct gatattcaag aagattataa
    11041 agttgaagac gttgacaaag aaatcattga ccaattactg aatgagtttc aaattgttga
    11101 aggttgggat agtttatcac agtatgaaca acggcaatac gtcctaaaac agttgggaga
    11161 acctttagat aatgctcaaa gttatagtga ctacccttct gcacagacaa attgcttact
    11221 ccaggtcaca agtcctaacc atattgctta tctggggttt aaccaaaaac caacgcaagg
    11281 cggtaaagct cttatttctc aaaaaatacg tgaccattta gataatgatg acggctggaa
    11341 aaaaggagag aatcccgcaa gaaaaagact atttaaaggt ggcactcccg tatcttacta
    11401 tgaacgagtt taaaaactac actaatacta cataaaaact acacgtaata acacaagcca
    11461 aaacattggt attataagct ttatactct
//
LOCUS       DATRQA010000058        11375 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.058, whole genome shotgun sequence.
ACCESSION   DATRQA010000058 DATRQA010000000
VERSION     DATRQA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11375
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.058"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            87..884
                     /locus_tag="VBD27_001143"
     CDS             87..884
                     /locus_tag="VBD27_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7025158.1"
                     /translation="MVRLIFSDIDGTLINSNFKVTPKTRQGIKQIVAQGATFVPISAR
                     MPEAITPIMEQIGIDSYIISYNGALIQDMQQKTIASHTMDGQVALQVCSYVSKHYSKI
                     AWNVYRYHEWYSCDKENEWVQKEEEIVGLQSKEMSLMELEKQDRIHKLLLMGEPSLMG
                     ELENTLKAQYPHLSIAQSAPYFIEIMAPGIEKGKSAKTLADYLDISLADSIAFGDNYN
                     DLNLLEIVGKGFVMGNAPKDLQERIGNVTQDNDNDGIYYALVEYMEN"
     gene            1349..2311
                     /locus_tag="VBD27_001144"
     CDS             1349..2311
                     /locus_tag="VBD27_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7025159.1"
                     /translation="MKKITKMVISSVLASVLMTACSQSSQNGQSDVKHIGILQYVEHP
                     SLTATRKGFIKELAKEGYKDGKNIKIEYKNAQGDQSNIQSISEKLIKDNKLVLGIATP
                     AAQSLTTVSTETPILFTAVTDPVSAELVKSMKKPEGLATGTSDMSPIKKQVSLLRKVM
                     PKVKRVGIMYTTSERNSEVQVKQAKKIFQEAGIKTSVKGISSTNDVQDTAKSLMSKTE
                     VLFVPTDNIIASSVTLLGNLSKELKVPVVGGSADMVPSGLLFSYGADYEALGCQTARQ
                     AVKILKGKDVAKVPSEYPQNLKVVVNEDMAKELGIDVSSIKNNK"
     gene            2327..3223
                     /locus_tag="VBD27_001145"
     CDS             2327..3223
                     /locus_tag="VBD27_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025160.1"
                     /translation="MLDIILSGISQGLLWSIMAIGVFITFRILDIADLSAEGAFPMGA
                     AVCALCIVNDINPIVATIAGMLGGMLAGLVSGFLHTKMKIPALLTGIITLTGLYSINL
                     LVLGRSNVSFALKNTLVTMVTRLGLNKLSAVLLIGIVCVGLVILILYLFLNTQLGLAL
                     RATGDNEAMGQANSIKVDRMKMLGYMIGNGLIALSGALLAQNNGYADLNMGVGTIVIG
                     LASIILAEVMIKYLPLGKRLWSIVLGSVLYRMIIVFILTTDIDAQMIKLVSAILLALI
                     LYVPELRAKLGVKASKTLTKEG"
     gene            3228..4031
                     /locus_tag="VBD27_001146"
     CDS             3228..4031
                     /locus_tag="VBD27_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7025161.1"
                     /translation="MSLLELVNLHKTFEKGTVNENHVLRGLDLTIEDGDFISVIGGNG
                     AGKSTLLNCIAGLIPIDQGTITLDNQSITKDSVEKRSKGISRVFQDPRMGTATNLTIE
                     ENMAIAHKRGNKRHIFRQSVTDDDRQLFKKSLSQLGLGLENRMKTDAAFLSGGQRQAL
                     TLAMATLVRPKLLLLDEHTAALDPKTSDMVMELTQKVIEEQRLTALMITHNMEHAIAY
                     GNRLVMLYHGKIVVDVKGEAKRNLTVAELMELFHKNSGQQLIDDALVLG"
     gene            4206..4490
                     /gene="groES"
                     /locus_tag="VBD27_001147"
     CDS             4206..4490
                     /gene="groES"
                     /locus_tag="VBD27_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO7025162.1"
                     /translation="MLKPLGDRVIISFVETEEKSVGGFVLAGASHDTTKTAKVLAVGD
                     GIRTLTGELVAPSVAEGDTVLVENGAGLEVKDGNEKVTVVRESDIVAVVK"
     gene            4586..6208
                     /gene="groL"
                     /locus_tag="VBD27_001148"
     CDS             4586..6208
                     /gene="groL"
                     /locus_tag="VBD27_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO7025163.1"
                     /translation="MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYI
                     LITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGA
                     GDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPEAPTAPAMDPSMMGGF"
     gene            complement(6240..6604)
                     /locus_tag="VBD27_001149"
                     /pseudo
     CDS             complement(6240..6604)
                     /locus_tag="VBD27_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6617..7354)
                     /locus_tag="VBD27_001150"
     CDS             complement(6617..7354)
                     /locus_tag="VBD27_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7025164.1"
                     /translation="MPKNELNNKLNKLKHVQVYNKIFNMIQDGTYSPGMQLPSEPELA
                     GQLNVSRATLRKSLALLQEDHLVKNIRGKGNFIRENSSNLSENGYENRQHPIKTCLTS
                     KITEVELEFRVEVPAEAITASLKQETPVVVIADRWYHTDDGPLAYTLSFIPIELISDA
                     EISLHDTKQLLNFIEEGIYHEGISSHSQSHLGYATSGNFSATKYTLSDHGQFILIQET
                     IFNQEKILMCNKHYVPIEHFELSITSH"
     gene            7513..8292
                     /gene="udp"
                     /locus_tag="VBD27_001151"
     CDS             7513..8292
                     /gene="udp"
                     /locus_tag="VBD27_001151"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEO7025165.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            8313..9515
                     /locus_tag="VBD27_001152"
     CDS             8313..9515
                     /locus_tag="VBD27_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEO7025166.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQVVSVVSTGVTKVINCGQAGLNFVFGSLADSGTKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILFPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            9545..10216
                     /gene="deoC"
                     /locus_tag="VBD27_001153"
     CDS             9545..10216
                     /gene="deoC"
                     /locus_tag="VBD27_001153"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO7025167.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVDEGVKIKAAGGISSLEDAEKFIALGASRLGTSRIIKIVKNQKV
                     EEGTY"
     gene            10283..>11375
                     /locus_tag="VBD27_001154"
     CDS             10283..>11375
                     /locus_tag="VBD27_001154"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO7025168.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGDPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIISAMCDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPS"
BASE COUNT         3621 a         1797 c         2409 g         3548 t
ORIGIN      
        1 attagaccag acgaagttta tttgcgaaac aaaaatttaa gtgttagact aaagtaagcg
       61 cgtacaatta tattttggag gataatatgg taaggctaat atttagtgat attgatggta
      121 ctttaattaa cagtaatttt aaagtaacac ctaagacacg gcagggaatt aaacaaatag
      181 tagctcaagg ggctactttc gttcccatat cagctaggat gcctgaggca attactccga
      241 ttatggaaca aattggaata gattcttata ttatttccta taatggggct ttaatacaag
      301 atatgcaaca aaaaacaatt gcttcccaca caatggatgg tcaagttgcc ttacaagttt
      361 gttcttatgt aagcaagcat tattcgaaaa ttgcatggaa tgtctatcgt tatcatgaat
      421 ggtattcctg tgataaagaa aatgagtggg tccaaaaaga ggaggagatt gttggtttac
      481 aatctaaaga gatgagtttg atggagttag agaagcaaga taggattcat aaacttctct
      541 tgatgggaga accatctctg atgggagaat tggagaacac tttgaaagct caatatccac
      601 atttatcaat cgcacaatct gctccttact ttattgaaat aatggctcca ggtattgaaa
      661 aaggaaaatc tgctaaaact ttagcagact atttagatat ttctcttgca gactctattg
      721 cgtttggtga taactataat gatttaaatc tgcttgaaat tgtgggtaaa ggttttgtca
      781 tggggaatgc tcctaaagat ttacaagaga gaattggaaa tgtaactcag gataatgata
      841 acgatggtat ttattatgct ttagtagagt atatggaaaa ttaaggatag caaatctaaa
      901 tttccttaaa agtgtgttat atcaacgaat gactattatt taaattatct atctaacaag
      961 aattttcaaa aaaatcaaat ttaactattg acaattattt ttaaatgacg taaacttata
     1021 ggcaaccata aacaaggaga gaataatatg ttaatcaaat cgtctattat taaacaaaca
     1081 aatacttatt attactttag tttctttaga tagacgtttg tagcctcaag gatacaaacg
     1141 tcacgcaaag tttgtatcta tgaagctaaa gcattttatg atgaaagcag agccgattag
     1201 atcatttcaa taatctagtt ggttaaggca atattttttc gccctcccgt gcacagagga
     1261 attgagagaa ataattaacc aactagatgt agggtctagt tggttttttg ttttatggaa
     1321 aatttgatgg tattacaagg agatagtaat gaaaaaaata acaaagatgg taattagcag
     1381 cgttttagca tctgttctga tgacagcttg ttcacaatct tctcaaaatg gtcaatctga
     1441 tgtaaaacat attggtattt tacaatatgt agaacatccc tctctaactg ccactaggaa
     1501 aggatttata aaagaattag ctaaagaagg ctataaagat ggcaaaaata tcaaaattga
     1561 gtataagaat gctcaagggg atcagtcaaa tatccaatca atttctgaaa aactcattaa
     1621 agataataaa ttagtattgg gaattgcgac tccggcggct caatcgttga caacggtgtc
     1681 aacagaaaca cctattttat ttacagcagt gacagatccg gtatcagccg aattagtaaa
     1741 atcaatgaaa aaacctgaag gtttagcgac tggtacaagt gacatgtctc caattaaaaa
     1801 gcaagtatcc cttttgagaa aagtaatgcc taaggttaaa cgtgttggta ttatgtatac
     1861 cactagcgag cgtaactcag aagttcaggt aaaacaagca aaaaaaatct tccaagaggc
     1921 ggggattaaa acaagtgtta aaggtatttc ttccactaat gatgtacaag atactgctaa
     1981 gagtttaatg tctaaaacag aggttctttt tgtaccaaca gataatatta tagccagctc
     2041 agtaacgctg ttagggaatc tatcgaaaga attaaaagtg cctgttgtgg gaggttcagc
     2101 agatatggta ccaagtggtt tgctcttttc ttatggggct gattatgagg ctttaggttg
     2161 tcaaacggca agacaagctg ttaaaatcct caaaggtaaa gacgtcgcta aagttccttc
     2221 agaatatcct caaaatctaa aagtagtagt taatgaagat atggcaaaag agcttggaat
     2281 tgatgtttca agtattaaaa ataataaata atcagaaagg tattgtatgt tagatattat
     2341 cttatccgga atttcgcaag gattactttg gtcaattatg gcaattggcg tgtttatcac
     2401 ttttcgtatc ttagacatag ccgatctctc tgcagaaggg gctttcccta tgggggctgc
     2461 agtttgcgcc ttatgtatcg ttaatgatat taatccaatt gtggcaacca tagcgggcat
     2521 gttaggtggt atgttggcag gactagtatc gggttttctt catactaaaa tgaaaatacc
     2581 agccctccta acaggtatta ttacgttgac aggactttac tctattaatt tacttgtttt
     2641 aggaagatca aacgtctctt ttgctctcaa aaatactttg gttacaatgg taacaagatt
     2701 aggtttaaat aaactttcag cagttttatt aattggaatt gtttgtgtag gtttagttat
     2761 cttaattttg tatctctttt taaataccca actaggatta gctttaagag cgacaggtga
     2821 taatgaggct atgggacaag ccaatagtat taaagtagat cggatgaaaa tgctaggata
     2881 tatgattggg aatggcctga ttgctttatc tggtgcactt ttagcgcaaa ataatggcta
     2941 tgcagatttg aatatgggag tgggtacaat cgttattgga ttggcttcaa ttatcttggc
     3001 agaagtaatg ataaaatact tacctcttgg taaaaggtta tggtcaattg tattaggttc
     3061 tgttttatac cgtatgatta ttgttttcat attaacaacc gatattgatg cacagatgat
     3121 aaagttggtt tcagcaattt tgttggctct cattttgtat gtcccagaat tacgagctaa
     3181 actaggagta aaagcctcta aaacgcttac aaaggaagga taagaacatg tcattacttg
     3241 aattagtcaa tctgcacaaa acttttgaaa aaggaactgt caatgagaac catgttttac
     3301 gtggtttaga tttaacgatt gaagacggtg attttatttc agtaattgga ggtaacggtg
     3361 cgggaaaatc aaccctcctt aactgtatcg ctggtctgat ccctattgat caggggacaa
     3421 tcacattaga caaccaatca attactaaag actctgtaga aaaacgatct aagggtatta
     3481 gtcgagtctt tcaggatcct agaatgggaa ctgcaacaaa tttgacgatt gaagaaaata
     3541 tggcaattgc acataaaaga ggcaataagc gtcacatttt tagacagtca gtgactgacg
     3601 atgaccgtca actatttaaa aagtctctat cacaattagg tctaggctta gaaaatcgaa
     3661 tgaagactga tgctgcattt ttatctggcg gtcagcgtca agccctcacg ttagctatgg
     3721 caactcttgt tcgtcccaaa ttattattac tggatgaaca tacagcggct ctagatccca
     3781 aaactagcga tatggttatg gaattaacac agaaagtgat agaagaacaa cgattgacag
     3841 ccttaatgat tacacacaat atggagcatg ccattgctta tggtaatcgt ctcgtgatgc
     3901 tctaccacgg gaaaattgtt gttgatgtta aaggagaagc aaagaggaac ttaacagttg
     3961 cagaactaat ggaacttttc cataaaaact caggtcaaca gttgatagat gatgcccttg
     4021 tcttaggtta aaatataaat ttctctagtg aatggacagg ataactaggg aaatttttct
     4081 tgtttgaaac gtgccttttc tcttgactaa ttctgaccaa gtgatagaat agtttttgta
     4141 gttagcactc tctttatagg aatgctaaaa aatcaaaata tatctcgtat ggaggttcgt
     4201 ttattatgtt aaaaccatta ggtgatcgtg tgatcatttc gtttgttgaa actgaagaga
     4261 aatctgtcgg tggttttgtt cttgcaggag caagccatga tacgacaaag acagccaaag
     4321 ttcttgctgt gggtgacggt attcgtacct taacagggga attagttgca cctagcgttg
     4381 cagagggtga tactgtttta gtcgaaaatg gtgctggttt ggaagttaaa gacggtaacg
     4441 aaaaagtgac agttgttcgc gaaagtgaca ttgttgctgt cgttaaatag gttttattaa
     4501 gcaagaaagg atataggcct attagagaaa agttgctttg taaagaaagt agactttgtt
     4561 aaattttagg cctgggtatt aaattatggc aaaagatatt aaattttcag cagatgcccg
     4621 ctcagcaatg gtgcgtggtg ttgatatttt agctgataca gtcaaagtaa cattaggtcc
     4681 taaaggccgt aatgttgttc ttgaaaaagc atttggttct cctttaatta caaatgatgg
     4741 tgtgacaatt gctaaagaaa ttgagctaga agatcacttt gaaaatatgg gagctaaact
     4801 tgtgtcagaa gtggcttcaa aaactaatga tattgcaggg gatggcacta caactgctac
     4861 tgttttgacc caagctattg tacgggaagg tcttaaaaat gtaactgcag gggcaaatcc
     4921 gattggcatt cgtcgtggta ttgaaacagc tgtttcagca gcagttgaag agctaaaaga
     4981 gattgcacaa ccagtttcag gcaaagaagc tattgctcaa gttgcggctg tgtcttcacg
     5041 ttctgaaaaa gttggggaat acatttctga agctatggag cgcgtgggta atgatggtgt
     5101 tatcactatt gaagaatcgc gaggtatgga aacagagctt gaagttgtgg aaggaatgca
     5161 gtttgaccgt gggtacttgt cacagtatat ggtaactgat aacgagaaaa tggtctctga
     5221 acttgagaat ccgtatatcc ttattacaga taagaaaatt tcaaatatcc aagaaatttt
     5281 accattatta gaagaggttc ttaaaacaaa tcgtccgttg ctaatcatcg ctgatgatgt
     5341 tgatggagaa gctctcccaa cgcttgttct taacaaaatt cgtggaactt tcaatgtcgt
     5401 agctgttaaa gcgcctggat ttggtgatcg tcgtaaagcc atgctggaag atattgctat
     5461 cctaacagga ggaactgtcg ttactgaaga ccttggttta gacttaaaag atgctactat
     5521 gcaagtttta ggacagtctg ctaaagtaac agtagataaa gattctactg ttattgtcga
     5581 aggtgccggt gactcatcag caattgctaa tcgcgtagcc atcattaagt cacagatgga
     5641 ggctacaact tctgattttg atcgtgaaaa attacaagaa cgacttgcta agttagccgg
     5701 tggtgtagca gtaattaaag ttggtgcggc gactgaaaca gaattaaaag agatgaaact
     5761 tcgtatcgaa gatgcgttaa atgcaacgcg tgctgcagtt gaagaaggta ttgtttcagg
     5821 tggaggtacg gctcttgtga acgttattga aaaagtagcg gcactgaaac ttaatggtga
     5881 tgaggagact ggacgtaata ttgttcttcg tgctctcgaa gagcctgttc gtcaaattgc
     5941 ttacaatgct ggatatgaag gttcagttat tattgaacgt ttaaaacagt ctgaaattgg
     6001 tacaggattt aatgcggcta atggagaatg ggtagatatg gttaccacag gtatcattga
     6061 tcctgtcaaa gtaacacgtt ctgcacttca aaatgcggca tctgtagcaa gtcttatctt
     6121 gactacagaa gcagtagtag caaataaacc tgaaccagaa gctcctacag ctcctgcaat
     6181 ggatccatct atgatgggtg gcttctaatc actaacttat cacaacataa aactcccctt
     6241 taacaaaggg gagttttatg ttgtgataat agagatatga tagtcactac cacttgcggt
     6301 aagaagatca taaggatagg ccatctaata tttgccagta gtgggaggaa aaaagtagca
     6361 aaacaatgat tgctattatc ttagattgag attgataagc taagaattga attatagcta
     6421 atagaattaa gtaactaata agtataaata acatagaaaa gagcattccg tacccagcac
     6481 ccattgacgg ttcctttgga ggggctagaa aataacttaa cttaaaataa gcgccaacta
     6541 ttaggcccat aattaattcg gtaaaagtta tgaaaaaagc aacttttaat agtctatttt
     6601 gcataggctt gcctccttaa tgagaggtaa tggacaattc aaagtgctca atgggaacgt
     6661 aatgcttatt acacattagt attttctctt ggttaaaaat tgtttcttgt attaatatga
     6721 attgtccatg atcggatagg gtgtatttag ttgctgaaaa attgccagat gtcgcatatc
     6781 ccaaatggct ttgtgagtgg cttgatatgc cttcgtgata gataccttct tcaatgaagt
     6841 ttaacaactg tttagtatca tgtaaagaaa tctcagcatc tgaaataagt tcgataggga
     6901 taaaagaaag tgtgtaagct aatggtccat catcggtgtg gtaccaacga tcagcaataa
     6961 caactacagg ggtttcttgt ttaagtgaag ctgtgatagc ttctgcagga acctctacac
     7021 gaaattctaa ttccacttct gtaattttgg aagttagaca agttttaatg gggtgttgtc
     7081 tattttcgta accgttttca gacaagttgc tagaattttc tctaatgaaa ttaccttttc
     7141 ctctgatatt tttaaccaaa tggtcttctt gtaatagtgc taatgacttt cgtaaagtag
     7201 ctcgactaac attaagttgc cctgctaatt cgggctcaga aggcaattgc attccaggag
     7261 aataagtacc gtcttgaatc atgttaaaaa tcttattgta gacttgcaca tgctttagtt
     7321 tatttagttt attatttagt tcatttttag gcattgtaac ctccgtatac aagttagttt
     7381 aattgaaaaa attagaaaaa tcaagttaaa aaataaaacg ttttctttcg ataaaagcgt
     7441 ttacaaaggt tgtcttgtat gttattatac aagtgtaaaa aaaatagaca agacattaag
     7501 gaggtcttta tcatgcagaa ttattcaggt gaagtcggat tgcaatatca cctacagatt
     7561 cgtccaggtg atgtaggtcg ttatgttatc atgccaggtg atccaaaacg ttgtgcaaaa
     7621 attgcagaac attttgataa tgcagttctt gtagcagaca gccgtgaata tgttacttat
     7681 acaggtacat taaatggtga aaaagtcagt gttacttcaa ctggtattgg tggtccatca
     7741 gcgtctattg cgatggaaga attgaaactc tgtggcgctg atacctttat ccgtgttgga
     7801 acatgtggtg gtattgacct tgatgttaaa ggcggagaca tcgttattgc aactggagct
     7861 atccgtatgg aaggtacaag taaagaatat gctccgattg aatttcctgc tgtagctgac
     7921 ttggaggtaa cgaatgctct agttaatgca gctaaaaagc ttggttacac aagtcacgca
     7981 ggagtagttc aatgtaaaga tgcattttat ggacaacatg agccagaacg tatgccagtt
     8041 agttatgaat tattaaataa atgggaagca tggaaacgtc tgggaacaaa agcgtctgaa
     8101 atggaatctg cagctctttt tgtagcagca agtcatctag gagttcgttg tggatcagat
     8161 ttccttgttg ttggtaatca agaacgcaac gctttgggta tggataaccc aatggctcat
     8221 gacacagaag cagctattca agttgctgtt gaagcactcc gcacacttat tgaaaatgat
     8281 aaatcacaat aagcgaggtt ttggaagtca tcatgcaatt tatttatagt attattggta
     8341 ttttattggt attaggaatt gtgtatgcaa tttctttcaa tcgtaagagt gtttctctaa
     8401 gtttaattgg aaaagctctt atcgttcaat tcattattgc gctaatctta gtacgtatcc
     8461 cactaggcca acaagttgtt agtgttgttt caactggagt tactaaagta atcaactgtg
     8521 gtcaagctgg tttaaatttt gtgtttggtt cattagcaga tagtggaaca aaaactggtt
     8581 ttattttcgc cattcaaacg cttggtaata ttgttttctt atctgcccta gttagtctac
     8641 tttattatgt aggaatcctt ggatttgtag taaaatggat aggtaagggt gttggtaaaa
     8701 ttatgaaatc ctcagaggtt gagagttttg ttgccgtagc taatatgttt cttggtcaaa
     8761 cagacagtcc aattttggtt agcaaatacc taggtcgtat gactgatagt gagataatgg
     8821 ttgtgttggt atcaggtatg ggaagtatgt cagtttctat tcttggtggc tacattgcat
     8881 taggcattcc aatggaatat ctcttgattg cttcaacaat ggttcctatt ggcagtattc
     8941 tcattgctaa aatcttattt cctcaaacag aacctgttca aaaaattgat gacattaaga
     9001 tggataataa aggtaataac gccaatgtga ttgatgcaat cgctgagggt gcaagcacag
     9061 gtgcacaaat ggctttctca attggtgcta gtttgattgc ctttgttggt ttagtttctt
     9121 tgattaatat gatgttaagt ggattgggaa ttcgcttaga acaaatcttc tcatatgttt
     9181 ttgctccatt tggttttctt atgggatttg accacaaaaa cattcttcta gaaggaaacc
     9241 ttcttggaag taagttgatt ttaaatgagt ttgtttcgtt ccaacaattg ggtcacctaa
     9301 tcaaatcttt agattatcgt acagcattgg tagcaactat ttcactctgt ggttttgcta
     9361 atttatcaag tttaggtatt tgtgtttcag gtattgctgt tctttgtcca gagaaacgtg
     9421 gcaccctagc tcgacttgtt ttccgtgcaa tgattggtgg tattgctgta agtatgctta
     9481 gcgcctttat cgtcggtatt gtaactctat tctaaaagtt gacaaaagaa agaaggatag
     9541 taacgtggaa gtaaaagata ttttaaaaac ggtagaccat actttgctag caacaacagc
     9601 aacatggcca gaaatccaaa caattttaga tgatgcaatg gcttatgaaa cagcttcagc
     9661 atgtattcca gcctcttacg tcaaaaaagc agcagagtac gtttcaggta aattagctat
     9721 ttgtactgtt attgggttcc caaatggcta tagtacaact gcagcgaagg tttttgaatg
     9781 tcaagatgct attaaaaatg gtgctgatga aattgacatg gtcattaatt tgacagacgt
     9841 taaaaatggg gattttgata ctgttgaaga agaaattcgt caaatcaaag ctgcatgtca
     9901 agaccatatc ttaaaagtta tcgttgagac atgtcaatta actaaagaag aacttatcga
     9961 actttgtgga gttgtcacac gttcaggtgc agactttatt aaaacctcta ctggtttttc
    10021 gacagcaggt gctacatttg aagatgttga agtgatggca aaatatgtcg acgaaggtgt
    10081 taaaattaag gcagcaggtg gaatctcatc attggaagat gctgaaaaat ttattgcttt
    10141 aggagcttca cgcttgggta ctagccgtat catcaagatt gttaagaacc aaaaagttga
    10201 agaaggcact tattaaataa tataaaggaa ataggaagga tgctggtctg acttcctatt
    10261 ttcaattgat aaaggagaag ttatgtcaca atttgatcgc attcacttag ttgtcttaga
    10321 ttcagtgggt attggagcgg caccagatgc caatgatttt gtcaatgcag gagttcctga
    10381 tggtgcttca gacacgcttg gccacatttc aaaaacagtt ggacttgcgg tgccaaatat
    10441 ggctaaaatt ggacttggta atattcctcg tccccaagcg cttaaaaccg ttcctgcaga
    10501 agaaaatcca agtggctacg cgacgaagtt acaagaagta tcacttggta aagatacaat
    10561 gacagggcat tgggagatca tgggcttaaa tattacagag ccatttgata ctttctggaa
    10621 tggttttcca gaagatatca tcacaaagat tgaggatttt tcaggacgta aagttattcg
    10681 tgaagcgaat aaaccttatt cagggacagc tgttattgat gacttcggac cacgccaaat
    10741 ggaaactggt gagctcatca tctatacctc agctgaccca gtcctacaaa ttgcagctca
    10801 tgaggatatc atccctctag aagaacttta ccgtatttgt gaatatgctc gtagtatcac
    10861 aatggagcgt ccagctctcc ttggtcgtat tatcgctcgt ccatatgtag gtgacccagg
    10921 caactttacg cgtacagcca accgtcacga ctatgcagta tcaccatttg aagataccgt
    10981 attaaataaa ctagatcaag ctggcatcga tacttatgct gttggtaaaa tcaatgatat
    11041 tttcaatggt tcaggcatta atcatgatat gggacataac aaatcgaata gtcatggtat
    11101 tgatactttg ataaaaacaa tggggttatc agagtttgaa aaaggcttct cctttactaa
    11161 tttggttgac tttgatgctc tctatggtca ccgtcgtgat cctcatggct atcgtgattg
    11221 tcttcatgag tttgatgagc gcctcccaga gatcatttca gcaatgtgtg acaaagactt
    11281 acttttgatc actgccgatc atggtaatga cccaacttat gcaggtacag accatacaag
    11341 agaatacatt cctctcttag cctatagccc atctt
//
LOCUS       DATRQA010000059        11366 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.059, whole genome shotgun sequence.
ACCESSION   DATRQA010000059 DATRQA010000000
VERSION     DATRQA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11366
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.059"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..788
                     /locus_tag="VBD27_001155"
     CDS             <1..788
                     /locus_tag="VBD27_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO7025169.1"
                     /translation="LGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKEATEQLN
                     KKTKQALKADKKAFPQLDKAVAKNEAQVLIKTSKGDINIKLFPKYAPLAVENFLTHAK
                     EGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNSKDKKKDSGNGFVNEISPYLYNI
                     RGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSLDGGYTV
                     FGQVISGMETVDKIASVEVTKSDQPKEKITITSIKVIKDYKFK"
     gene            complement(840..1673)
                     /locus_tag="VBD27_001156"
     CDS             complement(840..1673)
                     /locus_tag="VBD27_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO7025170.1"
                     /translation="MFTKFFEGLLTYHFLQNAFITAIVIGIVAGAVGCFIILRSMSLM
                     GDAISHAVLPGVAISFILGINFFIGAIVFGLLSSIIITYIKENSVIKGDTAIGITFSS
                     FLALGIILIGLANSTTDLFHILFGNILAVQDSDKYMTIIVGLIVLTLITIFFKELLLT
                     SFDPVLAKSMGMRVSFYHYLLMILLTLVAVTAMQSVGTILIVALLITPAATAYLYVKS
                     LRTMLFLSSALGAVASVLGLYIGYTFNIAAGSSIVLTSTFMFLLAFLFSPKQSLFKKK
                     Q"
     gene            complement(1675..2391)
                     /locus_tag="VBD27_001157"
     CDS             complement(1675..2391)
                     /locus_tag="VBD27_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO7025171.1"
                     /translation="MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLM
                     KALLGLVDSTGESGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGLYKERGLF
                     KRLSKTDWEKVSRVIDQVGLRGFENRPINALSGGQFQRMLMARCLVQEADYIFLDEPF
                     VGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQA
                     FTRENLSAAYGDAILLGQGD"
     gene            complement(2562..3488)
                     /locus_tag="VBD27_001158"
     CDS             complement(2562..3488)
                     /locus_tag="VBD27_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEO7025172.1"
                     /translation="MKKWLVIVSCFVAFLGLGACANKQDQAKNEKLKVVVTNSILADI
                     TKNIAGNKIDLHSIVPVGKDPHEYEPLPADVEKTTQADLVFYNGINLETGGNAWFTKL
                     IKNAKKKENKDYFAVSDGVDVIYLNGQSGKGKEDPHAWLNLENGIIYSKNIAKQLIAK
                     DPKNKATYEKNRDAYVAKLEKLDKEAKSKFNAIPANKKLIVTSEGCFKYFSKAYGVPS
                     AYIWEINTEEEGTPDQITSLVKKLKQVRPSALFVESSVDKRPMKSVSRESGIPIYAEI
                     FTDSIAKKGQKGDSYYAMMKWNLDKIAEGLAK"
     gene            3655..4302
                     /locus_tag="VBD27_001159"
     CDS             3655..4302
                     /locus_tag="VBD27_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO7025173.1"
                     /translation="MTPNKEDYLKCIHELGEKNNKISNKKIAEMMQVSAPAVSEMIKK
                     MISEQLIVKDKDLGYYLTKQGLLVVSDLYRKHRLVEVFLVNHLHYTADDIHEEAEVLE
                     HTVSTTFVDQLEKLLDFPQFCPHGGTIPKKGEFLVEINQMTLDQISQLGTYVISRVHD
                     DFQLLKYLEQHRLHINDTIELTQIDPYAKTYHITYNDENLTIPERIASQIYVSAT"
     gene            complement(4342..5031)
                     /locus_tag="VBD27_001160"
     CDS             complement(4342..5031)
                     /locus_tag="VBD27_001160"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEO7025174.1"
                     /translation="MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELV
                     LVQSGVGKVMSAMSVAILVESFKVDAIINTGSAGAVATGLNVGDVVVADTLVYHDVDL
                     TAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMTSKVGLIATGDSFIAGQEKIDV
                     IKGHFPQVLAVEMEGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRS
                     AQVLMAFLKAL"
     gene            complement(5041..5310)
                     /locus_tag="VBD27_001161"
     CDS             complement(5041..5310)
                     /locus_tag="VBD27_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025175.1"
                     /translation="MGKPLLTDDMIERSNRGEKVSGQTILDQETKIISTEDGMEQLTD
                     ENGKHIYKSRRIENAKRNEFQRKLNLVLFILLILLALLFYAIFKL"
     gene            complement(5310..5864)
                     /locus_tag="VBD27_001162"
     CDS             complement(5310..5864)
                     /locus_tag="VBD27_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7025176.1"
                     /translation="MDFEEKTINRQTVFDGQIIKVAVDDVELPNGLGQSKRELVFHGG
                     AVATLAVTPEHKIVLVKQYRKAIEGISYEIPAGKLETGESGSKEEAALRELEEETGYT
                     GNLEILYSFYTAIGFCNEKIVLYLATDLQKVENPRPQDDDEVLELLELSYEDCMQMVE
                     KGMIQDAKTIIALQYYGLKMGGHS"
     gene            complement(5885..7264)
                     /gene="glmU"
                     /locus_tag="VBD27_001163"
     CDS             complement(5885..7264)
                     /gene="glmU"
                     /locus_tag="VBD27_001163"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO7025177.1"
                     /translation="MSNYAIILAAGKGTRMKSDLPKVMHKVSGITMLEHVFRSVQAIE
                     PSKIVTVIGHKAELVRDVLGDKSEFVMQTEQLGTGHAVMMAEEELATSKGHTLVIAGD
                     TPLITGESLKNLIDFHVNHKNVATILTADAANPFGYGRIIRNSDDEVTKIVEQKDAND
                     FEQQVKEINTGTYVFDNQSLFEALKDINTNNAQGEYYLTDVIGIFKEAGKKVGAYKLR
                     DFDESLGVNDRVALATAEKVMRHRIARQHMVNGVTVVNPDSAYIDIDVEIGEESVIEP
                     NVTLKGQTKIGKGTLLTNGSYLVDAQVGNDVTITNSMVEESIISDGVTVGPYAHIRPG
                     TSLAKGVHIGNFVEVKGSQIGENTKAGHLTYIGNAEVGCDVNFGAGTITVNYDGQNKF
                     KTEIGSNVFIGSNSTLIAPLEIGDNALTAAGSTITDNVPIDSIAIGRGRQVNKEGYAN
                     KKPHHPSQK"
     gene            complement(7508..7921)
                     /locus_tag="VBD27_001164"
     CDS             complement(7508..7921)
                     /locus_tag="VBD27_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7025178.1"
                     /translation="MVKALETYIVTNGNGRQAVDFYKDVFQADLVNMMTWEEMDPNCL
                     EDRKDLIINAQLIFDGIRLQISDENPDFVYQAGKNVTAAIIVGSVEEAREIYEKLKKS
                     AQEVQLELQETFWNPAYANLVDQFGVMWQISTELN"
     gene            complement(7934..8311)
                     /locus_tag="VBD27_001165"
                     /pseudo
     CDS             complement(7934..8311)
                     /locus_tag="VBD27_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8400..9356)
                     /locus_tag="VBD27_001166"
     CDS             complement(8400..9356)
                     /locus_tag="VBD27_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7025179.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIEGIDLVAILSTVRSLETAKDLA
                     KEYNMSLATSEYKAVLDNEEIDTVYIGLPNHLHFDYAKEALLAGKHVICEKPFTLEAS
                     QLEELVSIANTRQLILLEAITNQYLPNFDLVKEHLSNLGDIKIVECNYSQYSSRYDAF
                     KRGEIAPAFNPEMGGGALRDLNIYNLHLVIGLFGEPITAQYLPNIERGIDTSGVLVLD
                     YGHFKTVCIGAKDCSAEVKSTIQGDKGSIAILGPTNTMPKISLTMNGQESHVYQLNGD
                     RHRMHDEFVIFEGIISNLDFKRAAQALEHSRTVMKVLDMVTK"
     gene            complement(9353..9805)
                     /locus_tag="VBD27_001167"
     CDS             complement(9353..9805)
                     /locus_tag="VBD27_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEO7025180.1"
                     /translation="MKAQELWNKFKSLNPSIGDEIDAWQFGAEPDLLAKLVVDGTKTA
                     TASAYDLYELDEEGLPQVGSYDIILDSNDEAICIIEITKVDIVPFKDVSADHAFKEGE
                     GDKTLEWWRKAHIDFFKPYFEEFGLMFSEDSRIVLEEFQVVYPKEINE"
     gene            complement(9802..10047)
                     /locus_tag="VBD27_001168"
                     /pseudo
     CDS             complement(9802..10047)
                     /locus_tag="VBD27_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
     gene            complement(10044..10742)
                     /locus_tag="VBD27_001169"
     CDS             complement(10044..10742)
                     /locus_tag="VBD27_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO7025181.1"
                     /translation="MTKVVLVTGCASGIGYAQAQYFLKQGYQVYGVDKSDKPNLNGNF
                     NFIKLDLSSDLSPLFTMVPTVDILCNTAGILDAYKPLLEVSDEELEHLFDINFFVTVR
                     LTRHYLRRMVEKKSGIIINMCSIASFIAGGGGAAYTSSKHALAGFTRQLALDYAKDCI
                     QIFGIAPGAVQTAMTASDFEPGGLAEWVASETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            complement(10735..10971)
                     /locus_tag="VBD27_001170"
     CDS             complement(10735..10971)
                     /locus_tag="VBD27_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEO7025182.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGDAENYVQLFDTLEVNGKVLQAT
                     PYFLINVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            complement(11061..11309)
                     /locus_tag="VBD27_001171"
     CDS             complement(11061..11309)
                     /locus_tag="VBD27_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025183.1"
                     /translation="MEKITNDFEKVIAVLNQLSISYELVENGPVLLTEQADNFIVGIE
                     GVRTKIMFLTNKKKTKYYLLIMDDQKLFYPMKTIVAFR"
BASE COUNT         3706 a         2317 c         1617 g         3726 t
ORIGIN      
        1 atcttggttt agcctgtgtc agtatattaa ctttaagtgg ttgcgagtca attgagcgtt
       61 ctctaaaagg agaccgttat gttgaccaaa aactagctga aaactcttca aaggaagcta
      121 ctgaacaact aaacaaaaaa actaagcaag ccttaaaagc tgataaaaaa gcttttcctc
      181 aattggacaa ggctgttgcc aaaaacgagg cacaagtgct tataaaaacc tctaaaggtg
      241 atataaatat caagttattt ccaaaatatg ctcctttagc tgttgaaaat tttctaacac
      301 atgcaaaaga aggatactat aatgggttaa gtttccacag agttatcaaa gattttatga
      361 tacaatctgg tgaccctaat ggagacggga ccggtggaaa atctatatgg aatagcaaag
      421 ataaaaagaa agattctgga aatggttttg ttaatgaaat atccccttac ctctacaata
      481 tcaggggaag tttagcaatg gcaaatgctg gagctgatac taatggcagc caattcttta
      541 tcaatcaaag ccagcaagat cattccaaac aactgtcaga taaaaaagtt cctaaggtga
      601 ttattaaagc ctattcagag ggaggaaatc caagtttaga tggtggttat accgtctttg
      661 gacaagtaat ctctggcatg gaaactgtgg ataaaattgc ttcggtagaa gttacaaaat
      721 cagatcaacc aaaagaaaaa attactatta caagtatcaa agttattaaa gactacaaat
      781 ttaaataatt ctatcaaaaa tgagactaga acattttcta gtctcatttt ttaacaacac
      841 tattgtttct ttttaaacaa actttgttta ggagaaaata ggaaagcaag taaaaacata
      901 aaagtagatg ttaaaacaat acttgatcct gcggcaatat taaatgtata accgatatat
      961 aaccccagaa cagatgctac tgctcctaat gcagatgata aaaacaacat tgttctgaga
     1021 cttttaacat aaagatatgc tgttgcagct ggtgtaatta aaagtgctac gatcaatatt
     1081 gttcccacac tctgcatagc tgttactgca actaatgtta acaaaatcat caataaataa
     1141 tgataaaaag aaactctcat tcccattgat tttgctaaaa caggatcaaa agaagtcaat
     1201 aataattctt taaagaagat agtaatcaga gtcagtacaa ttaatcccac aataatagtc
     1261 atatatttat cactatcttg tactgccaaa atattcccaa aaagaatatg aaagaggtca
     1321 gttgtactat tagcaagtcc aattaaaatg atacctaagg ctaaaaagga cgaaaaagta
     1381 attccaatag cagtatctcc ttttatgaca ctattttctt taatataagt aatgataatc
     1441 gaagacaaca aaccaaagac aatagcacct ataaaaaagt taatccctaa aataaaagat
     1501 atagcaacac ctggtagaac agcgtgagaa atagcatcac ccatcaaaga cattgagcgc
     1561 aagataataa aacagcctac tgcacccgct acaataccaa tcacaattgc agttataaaa
     1621 gcattttgca aaaaatgata agtcaataag ccttcaaaaa atttcgtaaa cattctagtc
     1681 tccttgtcct aacaaaatag catcaccgta agcagcactt aaattctcac gggtaaatgc
     1741 ctgatctatt ggtccacaag caattagatg acggttcaaa ataattactt gatcaaaata
     1801 atgatcaact ttactcaaat cgtgatggac gactaaaatt aattttccgg ccttacttaa
     1861 tttttttagt aaattaacta tgatttgttc actaatggaa tcaatcccta caaacggctc
     1921 atctaaaaaa atgtaatccg cttcttgaac gagacatctt gccataagca tacgttgaaa
     1981 ttgtccacct gataaggcat taattggtct attttcaaac cctctcaaac caacttgatc
     2041 aatcactcgg gatacttttt cccagtcagt ttttgatagt cgcttgaaga ggcctcgttc
     2101 cttatataat cctaaagaaa cgcattctcc aacagttata gggaattggt aatcaatatt
     2161 agttttttgt tcaacatatg ctactctccc cattaaaggt agcaaatctc ccccaatccc
     2221 tgattctcca gtagaatcaa ccaaaccaag aagagctttc ataagcgtag atttccccgc
     2281 cccattaggc cctaaaatac caatgattcc ggagccttct aagcgtaaat taatatcttc
     2341 tagcactaaa ttattatcat aacttacgct taagtgttta ctaataatca tttttcctcc
     2401 aaaagtagaa tggagctggt tataaaacca gactccactt ctctgtattt attaaagcta
     2461 agtcagtttt tagattagga tttcgacatt gttacaactc agccttaagc gagaatacta
     2521 catcattgat ttcattatga tttcaatgtc gattctcact cttattttgc taaaccttct
     2581 gcaatcttat caagattcca tttcatcata gcgtagtagc tgtcaccctt ttgacctttt
     2641 ttagcaattg agtctgtgaa gatttctgcg taaattggga tacctgattc tcttgaaaca
     2701 gatttcatag gacgcttgtc aacacttgat tcaacgaata aagctgaagg tcttacttgt
     2761 tttaatttct taactaagct agtaatttga tctggtgtac cttcttcttc agtgttaatt
     2821 tcccaaatgt atgctgatgg aactccataa gcttttgaga aatatttaaa gcaaccttca
     2881 ctagtaacaa ttaatttttt attagctgga atggcattaa atttagattt tgcttcttta
     2941 tccaattttt ctaatttagc tacataagca tctctgtttt tctcataagt agctttgttt
     3001 tttggatctt tagcaattaa ttgcttagca atattttttg agtaaataat accattttca
     3061 aggttcaacc aagcatgtgg gtcttcttta ccttttccgc tttgaccatt tagataaata
     3121 acatcaactc catcgctaac cgcaaagtaa tctttgtttt ctttcttctt agcgttcttt
     3181 attaattttg taaaccaagc attaccgcct gtttcaagat taattccgtt gtaaaaaaca
     3241 aggtctgctt gagttgtttt ttcaacgtct gctggtaatg gctcatattc atgaggatct
     3301 ttaccaacag gtacaatact atgtaagtcg attttgttac cggctatatt ttttgttata
     3361 tctgccaaaa tagagttggt tacaactact tttaattttt cgtttttggc ttgatcctgc
     3421 ttgttagcac aggcacctaa gcctaaaaat gctacaaaac atgatacaat aactaaccac
     3481 tttttcatca aaatgctcct tttaaaatta atataagtta tgtattaaat taagttaact
     3541 taattttgat attagcataa cctattataa agtatttgtc aaatactttt tcagtatttt
     3601 ttgatataat ggtcttagaa aaatagattt taagaagtat aagaaagact gaatatgact
     3661 ccaaataaag aagattacct aaaatgcatt catgaattag gtgaaaaaaa caacaaaatt
     3721 tccaataaga aaatcgctga aatgatgcaa gtctcggctc ctgccgtgtc agaaatgatt
     3781 aaaaaaatga taagtgaaca actgattgtt aaagataaag atttaggata ttatcttacc
     3841 aaacaaggtt tattagttgt ttctgacctt taccgtaaac atcgtctagt agaggtattt
     3901 ttagttaacc atttgcacta tactgctgat gatattcatg aagaagcgga agttttagaa
     3961 catactgtat caacaacttt tgttgatcaa ttagaaaagt tgttagactt tccacaattt
     4021 tgtccacacg gtgggactat tcctaaaaag ggagagtttt tagtagaaat taaccaaatg
     4081 acattggacc aaattagtca gcttggcaca tatgttatca gtcgtgttca tgatgatttt
     4141 cagctactga aatacctaga acaacaccgt ttgcatatca acgataccat tgaattgaca
     4201 caaattgacc cttatgcaaa aacttatcac atcacctata acgatgaaaa tctaacaatc
     4261 cctgagcgta tcgcaagtca aatatatgta tctgctacat aaataaaaaa gtccgtgtat
     4321 tcattagata cacgggcttt tttataatgc tttcaaaaat gccattaata cttgggcaga
     4381 gcgtttacca gcttcaatga taaattcatc aaatgtgata ttggcatcat gagctgcagt
     4441 atcactcata gcacgtacca ccacaaaggg ctttcctgta gcttgagcag cttgggcaat
     4501 tgctgcacct tccatctcga cagctaaaac ctgtggaaaa tgccccttaa taacatcaat
     4561 cttttcctgg ccagcaataa aactatcccc agttgcaatt aaaccaactt tactagtcat
     4621 ttcttcttta gataaaactg cttcaaaagt ggaaacaaaa gtcttatccg aatgaaaata
     4681 aagtggctgc attgacattt gtccatagtc ataaccaaag gcggttaaat ctacatcgtg
     4741 ataaactaat gtatctgcga caacgacgtc tcctacattc aatcctgttg ctactgcacc
     4801 agctgatcct gtattaataa tagcatcaac tttaaagctt tctactaaaa tagcaacact
     4861 catcgctgac atgactttcc caacaccaga ctgtactaaa acaagctcat gttcaccata
     4921 acgtccactg taatatacat ttgataatac tgtctcttga gatttatcct caagattttc
     4981 cacaaggagt tttaactctt cctccatggc tgcaataatt ccaattttca tgatatttcc
     5041 ttataattta aaaattgcat aaaacaagag agccaacaaa ataagcaaaa tgaataaaac
     5101 taaattcaac ttacgttgaa attcatttcg ttttgcattc tcaatccggc gactcttgta
     5161 gatatgttta ccgttctcgt cagttagttg ctccatgcca tcttctgtag aaataatttt
     5221 agtttcttga tctaagattg tttgacctga aactttttca cctcgatttg aacgttcaat
     5281 catatcatct gttaacaatg gttttcccat taagaatgac ctcccatttt taaaccgtaa
     5341 tattgtaaag caatgatcgt tttcgcatct tgaatcatgc ctttttctac catttgcata
     5401 caatcctcat aacttaactc tagaagctca aggacctcat catcatcttg tggcctagga
     5461 ttttcaactt tttgtaaatc agtagctaga tagaggacga ttttctcatt acaaaaacca
     5521 atagcagtgt agaaactata aagaatttca agattccctg tgtagccagt ctcttcttct
     5581 aattcccgaa gggcagcttc ttccttagaa cctgactcac ccgtctcaag tttccctgct
     5641 ggaatttcgt aagaaatacc ttcaatagcc ttacgatact gtttcactaa aacaatttta
     5701 tgctcaggag ttaccgccaa agtagcaaca gctcctccat gaaaaacaag ttcacgtttg
     5761 gattgtccca atccatttgg caactctaca tcatcaacag ctactttgat aatttgtcca
     5821 tcaaaaacag tttgacgatt aattgttttt tcttcaaaat ccataagaac ctcccaaata
     5881 agtgttactt ttgtgaagga tgatgtggtt ttttattggc atatccttct ttattgactt
     5941 gacgtcctct tccaatagct atgctatcaa ttggaacatt atctgtgatg gtagatccag
     6001 ctgctgttaa agcattatca ccaatttcaa gtggtgcaat cagagtagaa ttacttccga
     6061 taaagacatt acttccgatt tctgttttaa atttattttg tccatcataa ttgacagtaa
     6121 tcgtccctgc gccaaagtta acatcacaac ctacctctgc attgccgata taagttaaat
     6181 gacctgcctt cgtattctca cctatttgag aaccctttac ttcaacgaaa ttaccaatat
     6241 gaacaccttt tgcaagactt gttcctggtc taatatgcgc ataagggcct acggttactc
     6301 catcagaaat aatggattct tcaaccatcg aattagtgat ggtaacatca tttccgactt
     6361 gagcatctac taaataacta ccattggtta ataaagtacc tttaccaatc ttcgtctgtc
     6421 cttttaaagt cacattaggc tcaattacag attcttctcc aatctcaaca tcaatatcaa
     6481 tataagcgga atcaggatta accactgtga ccccattaac catatgttga cgtgcaattc
     6541 ggtgacgcat cactttttca gcagttgcaa gtgctacacg atcattaact cctagacttt
     6601 cgtcaaagtc acgaagttta taggcaccta cctttttacc cgcttctttg aaaattccaa
     6661 taacatcagt tagataatac tctccttgag cgttatttgt attaatgtct ttaagtgctt
     6721 caaatagact ttgattatca aaaacataag ttccagtatt aatctcttta acctgctgct
     6781 caaagtcatt agcatccttt tgttctacaa ttttagtaac ttcatcatct gaattacgga
     6841 taatacgacc atatccaaat ggatttgcag catcggctgt taagattgtt gcaacgtttt
     6901 tatgattgac atgaaaatca attaagtttt ttaaactttc tccagtaatc aggggtgtat
     6961 cacctgcaat aactagtgta tgtccttttg atgtcgctaa ttcttcttcc gccatcatta
     7021 cagcatggcc agttcctagc tgttctgttt gcattacaaa ctctgatttg tcacctaaaa
     7081 catcacgtac aagctccgcc ttatgtccaa taacagtaac aattttactt ggctcaatag
     7141 cttgcacact acggaaaaca tgctcaagca tggtaatacc agatacttta tgcataactt
     7201 ttggtaaatc agatttcatg cgagttccct tacccgcagc taaaataatg gcataatttg
     7261 acatattaat tcctctatct ctgaatataa ttcaacctat tatatcataa tgccttcaac
     7321 ttatcactaa taccccttta aatccctatt acaatagact ttagctaaaa tagcttactt
     7381 caattaggaa taaaatagct accttttggt agctattcaa tacatgaaat ccgttaattt
     7441 tttcttctct gtttacaatc agtattttta taaatctatc attgcaatgc tcagatggta
     7501 aaaacgatta gtttaactct gttgaaattt gccacatcac accaaattgg tctactaagt
     7561 ttgcgtaagc aggattccag aaagtttctt gtaactctaa ctgaacttct tgtgcactct
     7621 tctttaattt ttcataaatt tcacgcgcct cctctacgct acccacaata atagcggctg
     7681 taacattttt accagcttga tacacgaaat ctggattttc atccgagatt tgaagacgaa
     7741 tgccatcaaa aataagctga gcattaatga ttaaatcctt gcggtcttca agacagttgg
     7801 ggtccatctc ttcccatgtc atcatattca ctaaatccgc ttgaaagaca tctttataaa
     7861 aatcaaccgc ttgtcgccca tttccatttg ttacgatgta tgtttctagt gctttaacca
     7921 taagttctcc tttttatttt acttcacgga ttgcaaagta attattgtca ttatcggaaa
     7981 aattaaaaac tctcatactc cccatatcaa caataggatt tgtcataacc tcatttgctg
     8041 ttaattcttg atatgtactg tcaatatcag tagtttcaaa taaaatagag ggagttgcta
     8101 aatctagctc aggactcatt tgagctataa ttgctttgtc atgtaagacg aagctaactt
     8161 cactatccaa cttcggtgca actatatagg ttacataatc tccttgtgtc tatttttcaa
     8221 cacgctcaaa acccaccttg tttttccaaa aatctgctga tgcttcaaca ttgctaacgt
     8281 agagcattac ctgtccaatt gatgaaatca ttttattatc tcactttcgg ttttattact
     8341 aattagtata acatacagta tttaaaataa gtaaaaaatg actcaaaaga gtcatttact
     8401 tatttcgtta ccatatctag aactttcatc accgttctac tatgttctaa agcttgagca
     8461 gcacgcttaa aatctaaatt gctaataata ccttcaaaaa taacaaattc atcgtgcatg
     8521 cggtgcctat caccatttaa ttgataaaca tgactttctt gtccattcat agttaatgaa
     8581 attttaggca tagtattagt tggccctaaa atcgctatag accccttatc accttgaata
     8641 gtagatttta cttctgcact acaatctttt gctccaatac agactgtttt aaagtgccca
     8701 tagtctagga ctagaactcc tgaagtatca ataccacgct ctatatttgg taaatactgt
     8761 gctgttatag gttctccaaa aagtccaatt actaaatgta aattataaat attaaggtct
     8821 ctcaacgcac ctccacccat ttcaggattg aaagctggtg ctatttcccc acgtttaaag
     8881 gcatcataac gcgatgaata ttgggaataa ttacattcta caattttaat atctccaagg
     8941 ttgcttaaat gttcttttac aaggtcaaaa ttaggtaaat attgatttgt aattgcttcg
     9001 agtaatatta gttgcctagt attggcaata gatacaagtt cttctaattg acttgcttct
     9061 aatgtaaatg gcttctcaca aatgacatgt tttcctgcca agagtgcctc cttagcatag
     9121 tcaaaatgca agtgattcgg cagtccaata tagactgtgt caatctcctc attgtccaag
     9181 acagccttat attcactcgt agctagagac atgttatatt ccttagctag atctttagct
     9241 gtctccaaac ttctaactgt tgataagata gcaactagat caataccttc aattttctgc
     9301 aagacaggca aaacttcctt aacaatcata cctgtcccca aaaccgctaa tttcattcgt
     9361 taatctcctt tgggtaaacc acttggaatt cctctaaaac tatcctagag tcctcactaa
     9421 acattaaacc aaactcctca aaataaggtt taaagaaatc aatgtgagct tttctccacc
     9481 attctaaagt tttatctcct tctccttctt taaaagcatg gtctgcagag acatctttaa
     9541 aagggacaat atcaacttta gttatttcaa taatacagat agcttcatca ttgctatcca
     9601 aaataatatc atatgaacca acttgtggta acccttcctc atctaattca tataaatcat
     9661 aggcagaggc cgttgcagtt tttgtcccat ctacaactaa ctttgccaga agatctggtt
     9721 ctgctccaaa ttgccatgcg tctatctcat caccgattga aggattcaaa gatttaaact
     9781 tattccataa ttcttgtgcc ttcatattac ctccgaagca attcccacaa caaacttaac
     9841 tgcgttactc atttttcttt gtcttttaaa tccttcttta acatctaaaa tataaacagt
     9901 ttgaccaatc caaacacgta aataaacaga ttttattaaa atatcacctg atattccttt
     9961 aacttcccgc tcagtaccat cagatgcttc aaattccgta cgtaataacc aagtattacc
    10021 acaaccaaat tcaatctatt ttctcatttc aaactccagc caccatcaat tttgacaatt
    10081 tcaccttgca ttgatctcgc ttttcctgaa gcaagaaatc cagttaattc agcaacttca
    10141 cttggtttag tccaacgacc aattggcgtc tcactagcta cccattctgc taatccacca
    10201 ggttcaaaat cactagcagt catagcagtt tgtacagcac ctggcgctat accaaaaatt
    10261 tgaatacaat cttttgcgta atccaaagcc aattgacgtg tgaatcccgc aagggcatgt
    10321 ttagaactag tatatgcagc tccaccgcca cccgctataa aacttgctat tgagcacata
    10381 ttgataataa tacctgactt cttttccacc atacgtctaa gataatgacg tgttagccta
    10441 acagtcacga aaaaattaat atcaaatagg tgttccagtt cttcatcact tacttctaga
    10501 agtggcttgt aggcatctag aatacctgca gtattacaca aaatatcaac agtcggtacc
    10561 attgtgaata gtggggataa atcagaggat aaatccaact ttataaagtt aaaattacca
    10621 tttaagttcg gtttatccga cttatccaca ccatatactt gatacccttg tttcaaaaaa
    10681 tattgagcct gtgcataacc aattccacta gcacagcccg tcactaaaac aactttagtc
    10741 attgacctcg atccaatcct ctgctaaaac atcacatggt gttggtgccc acatggaaaa
    10801 accttcacct tcaccagtaa cattaataag aaaataaggt gtagcctgta gtactttccc
    10861 attaacttca agggtatcaa ataactgaac ataattttca gcgtcccccc aacctgtacg
    10921 tacatatttt ttctttgctt ttaaacctgg taaaatttct tcaaatgtca tattgttctc
    10981 ctttattgtc tttaaccatt gatatatcaa ggtttattgc ttatttaaaa tcataattta
    11041 aagtttcttc gccttttagt tcatctaaag gctactattg ttttcatagg ataaaatagt
    11101 ttctggtcat ccattattag taaataatat ttcgtctttt tcttgttggt caagaacatt
    11161 atttttgttc ttactccttc aattcccaca ataaagttat ctgcttgctc cgtcaacaaa
    11221 acaggtccat tttccactaa ttcataagaa atacttagtt gatttagcac agctataact
    11281 ttttcaaaat cattcgtaat tttctccatt cttccataga gttagcataa ctatttgtag
    11341 actagagaac ctaaaattga tacact
//
LOCUS       DATRQA010000060        11085 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.060, whole genome shotgun sequence.
ACCESSION   DATRQA010000060 DATRQA010000000
VERSION     DATRQA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11085
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.060"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1060)
                     /locus_tag="VBD27_001172"
     CDS             complement(<1..1060)
                     /locus_tag="VBD27_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7025184.1"
                     /translation="MQKMTKLRRFRFPLRFYFTLMFVLTMLFSVLASLLLVAAIVFTF
                     FQGVLTTHVLQVSALAVVFLSLVIASISMWYGSYHLTKPILDISHIVSNVAGGDFEGH
                     IYRNSNRRKSYEYYNELDELSESINQMIVSLSHMDHMRKDFITNVSHELKTPIAAVAN
                     IVELLQDPELDEETQSELLGLVKTESLRLTRLCDTMLQMSRVDNQETIGELSSVRVDE
                     QIRQAMISLTERWQAKRINFQLDSKPYTVYSNSDLLMQVWINLLDNAIKYSEDIVDLS
                     VRMEETNNHYLRVIISDKGRGISQYDVQHIFDKFYQADQSHNQQGNGLGLAIVKRIIV
                     LCKGHISVSSQLEIGTEFC"
     gene            complement(1044..1715)
                     /locus_tag="VBD27_001173"
     CDS             complement(1044..1715)
                     /locus_tag="VBD27_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7025185.1"
                     /translation="MIKILVVEDNIVQQKIITTKLTQEGYQFITASNGQEALNCLDTE
                     EVQLIITDIMMPMMDGYQLIQELRSAAYNVPIIVMTAKSQMEDMTKGFGLGADDYMVK
                     PVQLQELALRIKALLRRANIVAQHQLIIGNTCLNEDELSLKYFEQEIVFPQKEFRVLF
                     HLLSYPNRIFTRLELLDSIWGMDTDLDERVVDACINKIRRKVEHLPDFKIETVRGVGY
                     RAKND"
     gene            complement(1746..2432)
                     /locus_tag="VBD27_001174"
     CDS             complement(1746..2432)
                     /locus_tag="VBD27_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025186.1"
                     /translation="MSAILELKHISKHYPDGDELLSILDNLDLSVSAGEFVAILGPSG
                     SGKSTLLSIAGLLLGADQGSLYVNHENVTDLSQRQRTQLRREALGFIFQSHQLLPYLT
                     IQEQLQQEARFAKHYDKKTSLEEINKLLSDLGIEQCAHKYPNQLSGGQKQRAAIARAF
                     INHPKVILADEPTASLDEERGRQVTELIRQEVKSHNTAAIMVTHDERVLDLVDTVYRL
                     KDGKLVKENF"
     gene            complement(2432..3520)
                     /locus_tag="VBD27_001175"
     CDS             complement(2432..3520)
                     /locus_tag="VBD27_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025187.1"
                     /translation="MSHAWKELTFYKKKYLLIELLIIVMMFMVVFLSGLANGLGRAVS
                     AAIENNPAQTYILNEGAEQVITSSVLTTKDQTDLNSLNLKDSTTLNIQRSSLTRQGHE
                     KKIDISYFAIDKDSFMAPTLSEGKQLTSYKKAIILNDSLKAEGIKLGDKVIDKSSSIS
                     LTVVGFVHNSMYGHGPVAFIDKDIYTEINKKINPQYQFLPQALVMKNDKSISHLPTQL
                     EAVSKKDVIQHIPGYSAEQSTLNMILWVLVVASAGILGVFFYIITLQKRHEFSVMKAI
                     GTKMSEIALFQLSQVIILALFGIIVGDRLAIALSYVLPAQMPFVINWQNIILVSFVFL
                     VIAMISSALSIVKVAKIDPVEVINGGGE"
     gene            3702..4688
                     /locus_tag="VBD27_001176"
     CDS             3702..4688
                     /locus_tag="VBD27_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7025188.1"
                     /translation="MSTIRQVAEKAGVSTSTVSRYISQNGYVSQKASQKIEQAIRELH
                     YVPNFLAQSLKTKKNQLVGLLLPDISNPFFPRLARGVEEFLKEQGYRVMLGNTNNKSH
                     LEEEYLNVLLQSNAAGIITTHDFTKNHPEIDIPVVVVDRVNQETQYGVFSDNKEGGKL
                     AAQAIWTAGATNILLIRGPLDKADNLNQRFQGSQNYLLNKGARFAIEDSASFDFAEIQ
                     IEAKTLLDHHPDIDSIIAPSDIHAIAYLHEILNRGKRIPEDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYIMGQKAAELIFKITNQLPITNKRIKLPVHYVERETLRRKIDE"
     gene            4681..5592
                     /gene="rbsK"
                     /locus_tag="VBD27_001177"
     CDS             4681..5592
                     /gene="rbsK"
                     /locus_tag="VBD27_001177"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEO7025189.1"
                     /translation="MSNIVIIGSISMDLVMETNRIAKEGETVFGQRFSMVPGGKGANQ
                     AVAIGRLSQDRDNITILGAIGEDSFGPILLDNLNKNHVTTDFVGTIPSSSGVAQITLY
                     NNDNRIIYCPGANGKVDTKKWSQEWSIIKEADLVVLQNEIPHQANMKIANFCKEHSIK
                     LLYNPAPSRKTDIEMLDKVDYFTPNEHECQELFPNQKLEDILATYPEKLIVTLGTKGA
                     IYSDGKESHLIPALETKAVDTTGAGDTFNGAFGYAISKKFKIANALRFATLAAHLSVQ
                     KFGAQGGMPTIKEMEDNQHYEKDWYIK"
     gene            5567..5965
                     /gene="rbsD"
                     /locus_tag="VBD27_001178"
     CDS             5567..5965
                     /gene="rbsD"
                     /locus_tag="VBD27_001178"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEO7025190.1"
                     /translation="MKKTGILNSHLAKLADDLGHTDQVCIGDLGLPVPNGIPKIDLSL
                     TSGIPSFQEVLDIYLENILVEKVILAEEIKEANSDQLSRLLAKLDNSVNIEYVSHNHL
                     KQMTQDVKAVIRTGENTPYSNIILQSGVII"
     gene            5981..7459
                     /locus_tag="VBD27_001179"
     CDS             5981..7459
                     /locus_tag="VBD27_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEO7025191.1"
                     /translation="MKIDMRNISKSFGTNKVLEKIDLELQSGQIHALMGENGAGKSTL
                     MNILTGLFPASTGTIYIDGEERTFSNPQEAEEFGISFIHQEMNTWPEMTVLENLFLGR
                     EIKTTFGLLNQKLMRQKALEAFKRLGVTIPLDIPIGNLSVGQQQMIEIAKSLLNQLSI
                     LVMDEPTAALTDRETENLFRVIRGLKQEGVGVVYISHRMEEIFKITDFVTVMRDGVIV
                     DTKETSLTNSDELVKKMVGRKLEDYYPEKHSEIGPVAFEVSNLCGDNFEDVSFYVRKG
                     EILGFSGLMGAGRTEVMRTIFGIDKKKSGKVKIDNQEITITSPSQAIKQGIGFLTENR
                     KDEGLILDFNIKDNMTLPSTKDFSKHGFFDEKTSTTFVQQLINRLYIKSGRPDLEVGN
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELADRGVPIVMVSS
                     DLPEILGVSDRIMVMHEGRISGELSRKEADQEKVMQLATGGK"
     gene            7461..8402
                     /gene="rbsC"
                     /locus_tag="VBD27_001180"
     CDS             7461..8402
                     /gene="rbsC"
                     /locus_tag="VBD27_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEO7025192.1"
                     /translation="MKNMMKYISELTTLIALAGLMVIITLINPNFLTTNNLLNLLLQV
                     TANGFIAFGMTFVILTGGIDLSVGSILALSSALTAGLIGHGVPVGIAIVLSVALGGIL
                     GMLNGLFISYGKLAPFIVTLATMTIFRGATLVYSNGNPITAGLSDSFLFQFLGQGYIV
                     GIPFPVILMFLTFIILYILLHKTAFGKSVYALGGNEKAAYISGIKLNKVKIIIYTISG
                     IMASISGLIITSRLSSAQPTAGASYEMDAIAAVVLGGTSLSGGKGRIIGTLIGALIIG
                     VLNNGLNIIGVSAFWQQVVKGIVILMAVLLDRFKVAK"
     gene            8455..9423
                     /locus_tag="VBD27_001181"
     CDS             8455..9423
                     /locus_tag="VBD27_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO7025193.1"
                     /translation="MKFGKKLGFLALLMSIVLILGACGKTGLGNSSGNSTKNVTKKSA
                     KDLKLGVSISTTNNPYFVAMKDGIDKYASNKKISIKVADAQDDAARQADDVQNFISQN
                     VDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAGKMAADY
                     AVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDILSSQSANFDRAKALNTTQ
                     NMIQGHKDVQIIFAQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISAT
                     IAQQPAKMGEIAIQAAIDHYKGKKVEKETISPIYLVTKDNVEKYNW"
     gene            complement(9463..10917)
                     /locus_tag="VBD27_001182"
     CDS             complement(9463..10917)
                     /locus_tag="VBD27_001182"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO7025194.1"
                     /translation="MANKIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPETHENYRQSERLELYQRYIDQLLAEGKA
                     YKSYVTEEELAAERERQELAGETPRYINEFIGMSETEKEAYIAEREAAGIIPTVRLAV
                     NESGIYKWTDMVKGDIEFEGSNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPQFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     SEAVFNFIALLGWNPGGEEEIFSREQLINLFDENRLSKSPAAFDQKKMDWMSNDYLKN
                     ADFESVFALCKPFLEEAGRLTDKAEKLVELYKPQLKSADEIVPLTDLFFADFPELTEA
                     EKEVMAAETVPTVLSAFKEKLVSLSDEEFTRDTIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIYLLGKEKSVQHIDNMLAKL"
BASE COUNT         3573 a         2081 c         1933 g         3498 t
ORIGIN      
        1 cacaaaattc tgttccaatt tccaactgac tggagacact aatatgtccc ttacaaagaa
       61 caattatacg tttaactatt gctagtccta atccatttcc ttgttgatta tgcgactgat
      121 ccgcttggta aaatttatca aatatatgct gaacatcata ttgagaaatt cccctacctt
      181 tatcagaaat aataactctc agataatgat tattagtttc ttccattcgt acacttaagt
      241 ctacaatatc ctcggaatat tttattgcat tatctaaaag gtttatccaa acttgcatca
      301 gcaagtcaga gtttgagtaa acagtatatg gttttgaatc cagttgaaaa tttatccttt
      361 tagcttgcca acgttctgtt aaactaatca ttgcttgacg aatttgttca tctacacgaa
      421 cagaggatag ctccccaata gtctcctgat tatccacgcg cgacatttgc aacatcgtat
      481 cacataatct tgttaacctt aggctctctg ttttaacaag acctaataac tcactttgtg
      541 tttcttcatc taattctgga tcttgtaata gttctacaat attagctact gcagcaatcg
      601 gagtctttag ctcatgagaa acattagtga taaagtcttt tcgcatatga tccatatgag
      661 ataaagaaac aatcatctga ttaatacttt cagatagctc atccaattca ttataatact
      721 cataggattt acgacgatta ctgtttcgat aaatatgccc ttcaaagtca ccgcctgcaa
      781 cattggacac aatatggcta atatctaaaa ttggttttgt taaatgataa gaaccgtacc
      841 acatactaat actagctatc acaaggctta aaaaaacaac agcgagagcg ctaacttgta
      901 atacatgagt agttaagact ccttgaaaaa aagtaaaaac tattgctgca actagtaaaa
      961 gagaagctag gactgagaaa agcatcgtta acacaaacat taatgtgaaa taaaatctta
     1021 acggaaaccg aaatcgcctt agcttagtca ttttttgcac gataccctac ccctctcacc
     1081 gtttcaatct taaaatctgg taagtgttct acttttcgtc taattttatt gatgcaagca
     1141 tctaccaccc gctcatctaa atcagtatcc attccccaaa tactatccaa tagttctaaa
     1201 cgtgtgaaaa tcctattagg atagctaagt aagtggaaaa gaaccctaaa ttctttctga
     1261 ggaaatacaa tttcttgctc aaaatatttc aagcttaatt catcttcatt aagacaagta
     1321 ttcccaataa ttagctgatg ttgagctaca atattagctc tcctcaagag tgccttaata
     1381 cgtaaagcta attcttgtaa ctgcacaggc ttaaccatat aatcatctgc accaaggcca
     1441 aacccttttg tcatatcttc catttgtgat tttgctgtca taacaatgat tgggacattg
     1501 taagcagctg acctgagttc ttgaattaat tgataaccat ccatcatggg catcatgata
     1561 tcagttataa tcaattgtac ttcttctgta tctagacaat ttagagcttc ttgtccattt
     1621 gaagcagtga taaattggta gccctcttgt gttaattttg tggtaatgat tttttgttga
     1681 acaatgttat cctctacaac taatatttta atcattaaat cacctctagc ttattgtaac
     1741 acaagttaga agttttcttt aactaatttc ccgtccttta aacgataaac ggtatctact
     1801 aaatctagaa cgcgctcatc gtgtgtcacc ataatagctg cagtattatg tgatttaact
     1861 tcctgacgaa taagttctgt aacttgacgc ccacgttctt catcaaggct agctgttggc
     1921 tcatctgcca agataacctt aggatgatta ataaatgctc tagcaattgc tgcgcgttgc
     1981 ttttgtcctc ccgataattg gttagggtat ttatgtgcac actgctctat tcctaaatct
     2041 gataataatt tattaatttc ttccaagctt gttttcttat catagtgttt tgcaaatcga
     2101 gcctcttgtt gcaactgttc ttggattgtt aaataaggaa gcagttgatg actttgaaat
     2161 ataaaaccta gtgcttcacg gcgcaattgt gttctttgtc tttgagacaa atccgtaacg
     2221 ttctcatgat tgacatacaa actaccttga tctgcaccaa gaagtaaccc tgcaatggat
     2281 aaaagtgtgc ttttacctga acctgagggt cccagtatag ctacaaactc acctgcagaa
     2341 actgataaat ctaaattatc taatattgat aaaagctcat ctccatctgg atagtgtttt
     2401 gagatgtgtt ttaattctaa gattgctgac attattctcc tcccccatta ataacttcga
     2461 ctgggtcgat tttagcaact tttacaattg acaaagcact actaatcatt gcaatgacta
     2521 aaaatacaaa cgatactaga ataatatttt gccaattaat aacaaatggc atttgagctg
     2581 gtaatacata agagagagca atcgccaatc gatctccaac aatgatacca aacagtgcca
     2641 atatgatgac ttgactcaat tgaaataggg caatctctga catttttgtc ccaatagctt
     2701 tcatgacgct aaattcatga cgtttttgaa gtgtgatgat gtagaaaaag acacctaaaa
     2761 taccagctga agctacgaca aggacccata aaatcatatt caaagtggat tgttcagcac
     2821 tataaccagg aatatgttga atgacatctt ttttactaac agcttctaat tgagttggta
     2881 aatgagatat acttttatca tttttcataa ctaatgcctg aggtaagaac tggtattgag
     2941 gattaatctt tttattaatt tcggtataaa tgtctttatc aataaatgcc acaggtccgt
     3001 gaccatacat actattatgg acaaatccaa cgactgttag tgatatagat gatgacttat
     3061 caatcacttt atcacctaac tttatccctt cagcttttaa actatcattt aaaataatag
     3121 cttttttgta acttgtcagt tgttttccct cagaaagtgt aggtgccata aaagaatctt
     3181 tatcaatagc aaaataagaa atatcaattt tcttttcatg tccttgtcga gttaaactcg
     3241 aacgttgaat atttaaagtg gtactatctt ttaaattcaa actgtttaaa tctgtctgat
     3301 ctttagttgt caatacagat gatgtaatca cttgttcagc accttcattc aaaatatagg
     3361 tttgagctgg gttattttca atagcagcac taacagctct acctaaaccg tttgctaaac
     3421 ctgataaaaa aactaccatg aacatcataa caatgattag caattcaatc aggaggtatt
     3481 ttttcttata aaaggttaat tctttccaag catgtgacat ggtttttctc ctttgatctt
     3541 ttgatgtgaa catagtatca cagctttatg acttgaatat gacctctaaa aataatgata
     3601 ttttatcatt aaattattta aaaaatagct tgacaatttt ttagtaagcg attacaattc
     3661 taattatgaa accgttccca caataaaaga aaggagtttc tatgagtacg attagacaag
     3721 tcgccgagaa agctggcgta tcaacatcta ctgtctcaag gtatatatcc caaaatggtt
     3781 acgttagtca aaaagcaagt caaaaaatcg agcaagctat ccgcgaactc cattatgttc
     3841 caaatttcct tgcacaatct ctgaaaacca aaaaaaatca acttgttgga ttactacttc
     3901 ccgatatttc aaaccctttc tttccaagac ttgctagagg agttgaagaa tttctaaaag
     3961 aacagggtta tcgtgtcatg cttggcaaca caaataacaa gagccattta gaagaagagt
     4021 acttaaatgt gctattacaa agtaatgctg cagggattat tacaacacac gatttcacca
     4081 aaaatcatcc tgaaatagat attccagttg tagttgttga ccgcgttaat caagaaacac
     4141 agtacggcgt tttctctgat aataaagagg gaggaaaatt agcagctcaa gctatttgga
     4201 cagcaggtgc taccaatata ttactcattc gtggtccgct agataaagct gataacctta
     4261 atcaaaggtt tcaaggaagc caaaattacc tattaaacaa aggggctcgt tttgctatcg
     4321 aagatagtgc cagttttgat tttgcagaga ttcaaattga agcgaaaact ttacttgacc
     4381 accatccaga tattgatagc attatcgctc catctgatat acatgctatt gcctacctac
     4441 acgagattct taatcgaggg aagcgaatcc ctgaagatgt acaaattatt ggttatgatg
     4501 atattttaat gagccaattt atctaccctt cgttgtcaac tattcaccaa tcatcatata
     4561 ttatgggaca aaaagcagct gaattaattt tcaaaattac taatcaatta cctattacca
     4621 ataagcgtat taaattaccc gttcattatg tcgaacgtga aaccctaagg aggaaaatag
     4681 atgagtaaca ttgtcattat cggtagtatt tccatggact tagttatgga aaccaatcgt
     4741 atcgccaaag agggagaaac cgtatttggc caacgttttt caatggttcc aggaggtaaa
     4801 ggtgctaatc aggctgtcgc tattgggcgt cttagccaag atcgagataa tattactatt
     4861 cttggtgcca ttggcgagga ttcttttggt cccattcttt tggataattt aaacaaaaac
     4921 catgttacta cggattttgt gggaacgata ccatcttctt caggtgttgc ccaaatcact
     4981 ctttataata atgataatcg aatcatttac tgtcctggag ccaacggaaa agttgatact
     5041 aaaaagtggt ctcaggagtg gtcaataatt aaagaagctg atttagttgt attacaaaat
     5101 gaaattcctc atcaagcaaa tatgaaaatt gctaattttt gtaaagaaca tagtattaaa
     5161 ttactctata atcctgcacc atctagaaag actgatattg agatgctcga taaggtcgat
     5221 tatttcacac caaatgaaca cgaatgtcaa gagctgttcc caaaccaaaa actagaggat
     5281 atattagcca catatcctga aaaactaatt gtaacactcg gcacaaaagg agccatctac
     5341 tctgatggaa aagaaagcca tttaattcct gccttagaaa ctaaagctgt tgatacaact
     5401 ggtgcaggtg atacttttaa cggtgcattc ggttatgcta tctcgaaaaa atttaagatt
     5461 gctaatgcct tacgttttgc aactctagct gctcacttat ccgtgcaaaa atttggtgca
     5521 cagggtggca tgcctacaat taaagaaatg gaggacaacc aacattatga aaaagactgg
     5581 tatattaaat agtcatctcg ctaaattagc agatgacctt gggcatactg atcaagtttg
     5641 tattggagat ctgggtctac ctgttccaaa tggcattcca aagattgatt tgtctttgac
     5701 ctctggaata cctagtttcc aagaggtact tgatatctat ttagagaata tcttagttga
     5761 aaaagtcatt ttagcagaag aaatcaagga agcaaattcg gatcaattat caaggttatt
     5821 agctaaactt gataactctg ttaacataga gtacgtcagt cacaatcatt taaaacaaat
     5881 gacccaagat gtcaaagcag ttattcgtac aggtgaaaat acaccgtatt caaatattat
     5941 tctacaatct ggtgttatca tctagaaagg agtttagtcc atgaaaattg acatgaggaa
     6001 tatctccaaa tccttcggta ctaataaagt tctagagaag attgatttgg aattacaatc
     6061 tggtcaaata cacgcactta tgggtgaaaa tggtgcgggt aaatcaacct taatgaacat
     6121 tctaacagga ctttttccag caagtacagg aactatttat attgatggcg aggaacgaac
     6181 tttttcaaac cctcaagaag cagaggaatt tggtattagt tttattcacc aagagatgaa
     6241 tacttggccg gaaatgactg tcttggaaaa tctctttctt ggacgtgaaa tcaagacaac
     6301 ttttggctta ctcaatcaaa aattaatgag acaaaaagca cttgaagctt ttaaaagatt
     6361 aggtgtgact attcccctag acatcccgat tggtaacttg tctgttggac aacaacaaat
     6421 gattgaaatc gccaaaagtc tcctaaacca actctctatt ttggtaatgg atgaaccgac
     6481 tgctgcttta acagatcgtg aaactgaaaa tcttttccga gttatccgag gtttaaaaca
     6541 agaaggagtg ggcgttgtct atatctccca tcgaatggag gaaattttta aaatcactga
     6601 cttcgtaacc gtcatgagag atggtgtcat tgtagataca aaagaaacat cactcacaaa
     6661 ttctgacgaa ttggttaaaa aaatggttgg ccgaaaattg gaagattact atcctgaaaa
     6721 acattctgaa attgggcctg ttgcttttga agttagtaac ctctgtggag ataatttcga
     6781 ggacgtttcc ttttatgtcc gtaaaggcga gattcttgga ttttctggat taatgggagc
     6841 tgggcgtact gaagttatgc gaactatctt tggaattgat aaaaagaagt ctggtaaggt
     6901 gaaaattgat aaccaagaaa tcactattac tagcccaagc caagctatca agcagggcat
     6961 tggttttttg accgaaaata gaaaagatga aggtctcatc ctagacttca atatcaagga
     7021 caatatgact ctccctagca ctaaagattt tagcaaacat ggtttctttg atgaaaaaac
     7081 aagcacaaca tttgtgcaac aattgattaa tcgcctctac attaaatcag ggagaccaga
     7141 tttagaggtt ggaaatcttt ctggaggtaa ccaacaaaag gtcgttctcg ctaaatggat
     7201 tggtattgct cctaaagtct taattcttga tgagccaaca cgtggtgttg atgtcggagc
     7261 taaacgagag atttaccaat taatgaatga actagcagac agaggtgttc caattgtcat
     7321 ggtatcatca gacttacctg aaattctagg tgttagtgac cgcatcatgg taatgcacga
     7381 aggtcgaatc agtggagaac tcagtcgaaa agaagctgac caagaaaaag ttatgcagct
     7441 agccacagga ggtaagtaaa gtgaaaaata tgatgaaata tatatcagag ttaacaaccc
     7501 tgattgcatt agctggttta atggttatta tcacactcat taatcctaat ttcctcacaa
     7561 ccaataatct actcaattta ttattacaag taactgcaaa tggttttatc gctttcggga
     7621 tgaccttcgt tattttgact ggcggtattg atttatcagt cgggtccata ttagccctat
     7681 ctagtgcatt aacagctgga ttaatcggcc acggagtacc cgttggtatt gctatcgttt
     7741 tatctgttgc cttaggtggc atcttgggaa tgctaaacgg actcttcatt tcttacggaa
     7801 aattagcacc atttatcgtt acactggcaa caatgactat ctttagagga gcaacgctag
     7861 tttattcaaa cggcaaccca attaccgctg gattatccga tagttttcta ttccaatttt
     7921 tgggacaagg ctatattgtt ggtatcccat tcccagtcat tctcatgttt cttactttta
     7981 ttattttgta tattttgtta cataaaacag ccttcgggaa atctgtttac gctttaggag
     8041 gtaatgaaaa agcagcgtat atctcgggta ttaagttaaa caaagttaaa attatcatct
     8101 acactatctc aggtatcatg gcttccattt ctgggttaat tatcacatca cgccttagct
     8161 cagcacaacc aacagctgga gcaagttatg agatggatgc tattgccgcc gttgtcttag
     8221 gtggaacgtc tttatctggt gggaaaggac gtatcattgg aacattgatt ggtgctctca
     8281 ttattggtgt cctgaacaat ggattaaaca tcattggtgt ctctgcattt tggcaacaag
     8341 ttgtgaaagg tatcgttatt ttaatggctg ttttacttga tcgctttaaa gtagcaaaat
     8401 agtctttact ctaatttcta taagttcatt atattttatc taaaggagtt tcaaatgaaa
     8461 tttggaaaaa aacttggctt tctagcctta ttgatgtcta ttgtcctcat tctcggtgca
     8521 tgtggtaaaa ctggacttgg aaattcttct gggaattcta caaaaaatgt aaccaaaaaa
     8581 tctgctaaag atttgaaact aggtgtatct atttcaacaa ctaacaaccc ctactttgtt
     8641 gcgatgaaag acggtattga caaatatgcg agcaataaaa aaataagcat taaggtagct
     8701 gatgctcaag atgacgccgc acgtcaagca gatgatgttc aaaactttat tagtcaaaac
     8761 gtagatgcta ttttgattaa tcccgttgac tcaaaagcta ttgtaacagc tattaagtct
     8821 gcaaacaacg ctaatattcc tgttattcta atggaccgtg gtagtgaggg aggcaaagta
     8881 ttaactactg tcgcttcaga taacgttgct gctggtaaga tggctgcgga ttatgccgtt
     8941 aaaaaactag gcaaaaaagc aaaagccttt gaattatctg gagtaccagg tgcctctgct
     9001 actgtagacc gtggtaaagg atttcattca gttgcaaaat ctaaacttga cattctttca
     9061 agccaatctg ccaattttga ccgcgccaaa gctttgaata ctacacaaaa tatgattcaa
     9121 ggacataaag atgtccaaat tattttcgca caaaatgatg aaatggcact tggggctgca
     9181 caggctgtca aatctgctgg cttgcaaaat gttcttatcg taggaattga tggtcagcca
     9241 gatgcccatg atgccattaa aaaaggtgat atttcagcca ctattgcaca acaaccagct
     9301 aagatgggag aaatagctat tcaagcagca attgatcatt ataaaggcaa aaaagtagag
     9361 aaagaaacga tttctccaat ttaccttgtt accaaagaca atgttgaaaa atacaattgg
     9421 taaaagtaca caaaaagtcg tgaaaattca cgacttttta tattataatt ttgctaacat
     9481 attatcgata tgttgaactg atttttcttt acctaataaa taaatagtat ctggtaactc
     9541 tggtccatgc atttcaccag aaaccgcaat acgaataggc ataaataggt tcttaccttt
     9601 gatgcctgtt tctttttgaa cagccttaat ttgagggaaa atagtatctc tagtgaattc
     9661 ttcgtcagaa agcgatacta acttttcctt aaaagcagat aagactgttg ggactgtttc
     9721 tgctgccatt acttcttttt cggcttctgt taattctgga aaatctgcaa agaaaaggtc
     9781 tgttaatggt acaatctcat cagccgattt taattgaggt ttataaagct caactaattt
     9841 ttcagctttg tctgtaagtc gaccagcttc ttctaagaat ggtttgcaaa gtgcaaaaac
     9901 actctcaaaa tcagcatttt ttaggtaatc attactcatc caatccatct tcttctgatc
     9961 gaatgctgct ggtgatttgc taagacgatt ttcatcaaat agattaatta attgctcgcg
    10021 tgagaagatt tcttcttctc caccaggatt ccaaccaaga agggcaatga aattaaagac
    10081 agcttccgac atataacctt ttttacgata gtcttcgata aattgaagag tatttgtgtc
    10141 gcgttttgat aatttcttac cagtctctga gtttataatc aatgtcatgt gaccaaattg
    10201 tggcgcttcc caaccgagtg cttcataaac cattaattgt tttggcgtat ttgcaatatg
    10261 gtcatcgcca cgaataacat gcgaaatttg catgtcatga tcatcaatga ctactgcgaa
    10321 attgtaagtt ggataaccat ctttcttctg aataacccag tctccgccga tattagagcc
    10381 ttcaaactca atgtcacctt ttaccatatc agtccactta taaatcccag attcattaac
    10441 agcaaggcgt actgtcggaa tgatgcctgc tgcttcacgt tctgcaatgt aagcttcttt
    10501 ttcagtttct gacattccga taaattcgtt gatatagcga ggtgtttcgc ctgccaattc
    10561 ttggcgttca cgctctgctg ctaattcttc ctcagtcaca taggacttgt aggctttacc
    10621 ttctgctaac aactgatcaa tataacgttg gtagagctca aggcgttctg attgacgata
    10681 attttcatgt gtttcagggc tttcatccca gtccatacct agccaacgta agttctccaa
    10741 ttgagagcgt tcaccatctt cgacgtgacg ttttcggtca gtatcttcaa tacgaataac
    10801 aaaatcccca ccatgatggc gagcataaag gtagttaaag agcgcagtac gcgcatttcc
    10861 gatgtgtaac aatcctgttg gacttggtgc ataacgcaca cgaatcttat tagccaaaat
    10921 tctactccta tattttctga tagtaaactt tttcagttta actgaattag tcaagggggc
    10981 aaaacatcat tcaaactctt acagatgatt gccaatcaac cctattatac cactaaatca
    11041 ctaaaaatga taatcagtaa taatagtcac actaattttc gataa
//
LOCUS       DATRQA010000061        10871 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.061, whole genome shotgun sequence.
ACCESSION   DATRQA010000061 DATRQA010000000
VERSION     DATRQA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10871
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.061"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(132..860)
                     /gene="lrgB"
                     /locus_tag="VBD27_001183"
     CDS             complement(132..860)
                     /gene="lrgB"
                     /locus_tag="VBD27_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HEO7025195.1"
                     /translation="MELLKTPIFGICFSLILYTIGQHLFKKSKGFFLLQPLFFAMVSG
                     IVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWFLNPATIAFAVPLYKKNDVVKKYWVE
                     ILSSLVIGMIVSLMLIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGGNTAVTA
                     MACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAA
                     IAVVVIGLVVDLVIPIFSHLIGLL"
     gene            complement(862..1317)
                     /locus_tag="VBD27_001184"
     CDS             complement(862..1317)
                     /locus_tag="VBD27_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO7025196.1"
                     /translation="MKHTSKETKTKEAPMFIQMSIYAAILLVSQMISMLLPKSLPIPT
                     TVIGLVLMYVLLTAKIIKVEWVDSFGALMISMIGFMFVPSGISVAANLDILKAEGLQL
                     VAVITISTVVMLVVVAYVARLILAIRELSVTDLFKKILPNHYSKKVEEK"
     gene            complement(1487..2227)
                     /locus_tag="VBD27_001185"
     CDS             complement(1487..2227)
                     /locus_tag="VBD27_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEO7025197.1"
                     /translation="MKILILDDEMFARQELSFLVEHSQEVDNPEIFQAEDISEAEKIL
                     FRQQIDLIFLDISLSEENGFTLANQLSQLAHPPLVVFATAYDNYAVKAFESNAVDYIM
                     KPFEQQRVDMALSKVKKLSQLTTASDVEQAIPKKASAELLTLTLSDRSVVVKMQDIVA
                     ASVEDGELTVSTVQKTYTIRKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWFNH
                     TLLLIMSNGEKFPVGRSYLKDLNEHLTM"
     gene            complement(2208..3953)
                     /locus_tag="VBD27_001186"
     CDS             complement(2208..3953)
                     /locus_tag="VBD27_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO7025198.1"
                     /translation="MLMVLLFQRLGIIMILAFLLVNNSYFRQLIEERSKRETVVLVII
                     FGLFVIISNITGIEIKGDRSLVERPFLTTISHSDSLANTRTLVITTASLVGGPLVGSI
                     VGFIGGVHRFFQGSFSGSFYIVSSVLVGIVSGKIGDKLKENHLYPSTSQVILISIIAE
                     SIQMLFVGIFTGWELVKMIVIPMMILNSLGSTLFLAILKTYLSNESQLRAVQTRDVLE
                     LTRQTLPYLRQGLTPQSARSVCEIIKRHTNFDAVGLTDRSNVLAHIGVGHDHHIAGQP
                     VKTDLSKSVIFDGEPRIAQDKAAISCPDHNCQLNSAIVVPLKINDKTVCALKMYFAGD
                     KTMSEVEENLVLGLAQIFSGQLAMGITEEQNKLASMAEIKALQAQINPHFFFNAINTI
                     SALIRIDSDKARYALMQLSTFFRTSLQGGQDREVTLEQEKSHVDAYMNVEKLRFPDKY
                     QLSYDISAPEKMKLPPFGLQVLVENAVRHAFKERKTDNHILVQIKPDGHYYCVSVSDN
                     GQGISDTIIDKLGQETVAESKGTGTALVNLNNRLNLLYGSVSCLHFSSDKNGTKVWYR
                     IPNRIREDEHENFNS"
     gene            complement(3980..4624)
                     /locus_tag="VBD27_001187"
     CDS             complement(3980..4624)
                     /locus_tag="VBD27_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7025199.1"
                     /translation="MEKVIIFDMDGVIVDSEYTFLDNKTEMLREEGIDTDVSYQYQYM
                     GTTFEFMWQAMKEEFGLPKTVKEYIAEMNRRRQAIVTRDGVRPIKGAQQLIHWLHQHG
                     YRLAVASSSPMVDIKRNLKELGVTECFEYMVTGEDVSSSKPAPDVFLRAAELLDVDPK
                     VCIVIEDTRNGSLAAKAAGMYCFGFANPDYPPQDLSMADKVISDYQDIYIYLPE"
     gene            complement(4748..5143)
                     /locus_tag="VBD27_001188"
     CDS             complement(4748..5143)
                     /locus_tag="VBD27_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7025200.1"
                     /translation="MYNKVIMIGRLTAKPEMVKTPTDKSVTRATVAVNRRFKGSNGER
                     EADFINVVMWGRLAETLASYGTKGSLISIDGELRTRKYEKDGQTHYITEVLASSFQLL
                     ESRAQRAMRENNVSGDLSDLVLEEEELPF"
     gene            5224..5940
                     /locus_tag="VBD27_001189"
     CDS             5224..5940
                     /locus_tag="VBD27_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO7025201.1"
                     /translation="MTNVTSYKEMLAKPWGKIQYEITFAQLSHIKNQNVLDFGAGFCL
                     TAQHLAKENNVTAIEPNPKLLYDNQSDNIYKILGSYEALRALPDQSFDTIICHNVLEY
                     IDKHNHPAYFDEFSRLLKPNGELSLIKHNIIGKILQSVIFSNDTSTAMELLTGEANFK
                     SASFDQGNIYTLEELKQNTNLLVERYQGIRTFYSLQPNHFKTETDWLNKMLAIELSVA
                     DKAPYKDIAFLQHITLKKSL"
     gene            complement(5994..6620)
                     /locus_tag="VBD27_001190"
     CDS             complement(5994..6620)
                     /locus_tag="VBD27_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEO7025202.1"
                     /translation="MIFTYNREHVGDTLMVIVKDSQGAKLDVDRRGQVARVYLQDSKE
                     TVAWNIFEVSSLIAIEGVGQVTLSDQVIKILNAELLKEGFEDSLVNNIEPTFVVAQIK
                     EMIDHPDSDHLHICQVEINDGKTVQIVCGAPNASVGLKTVAALPGAMMPNGSLIFPGK
                     LRGEDSFGMLCSARELALPNAPQVRGIIELSDQVIVGESFDANKHWKN"
     gene            complement(6653..6976)
                     /locus_tag="VBD27_001191"
     CDS             complement(6653..6976)
                     /locus_tag="VBD27_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO7025203.1"
                     /translation="MILPESYEEIAAYIDSTKKVVFFFTADWCPDCQFIYPVMPSIEK
                     DFSDFVFVRVNRDDYIELAQQWNIFGIPSFVVVENGQELGRLVNKNRKTKAEITKFLA
                     EINHK"
     gene            complement(6973..7257)
                     /locus_tag="VBD27_001192"
     CDS             complement(6973..7257)
                     /locus_tag="VBD27_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEO7025204.1"
                     /translation="MKNKKILFGTGLAGVGLLAAAGYTLTKKVTDYKRQQITQTLREL
                     FSQMGDIQVFYFNEFESDIKMTSGGLVLEDGRIFEFIYRQGVLDYVEVSK"
     gene            7418..7657
                     /locus_tag="VBD27_001193"
     CDS             7418..7657
                     /locus_tag="VBD27_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025205.1"
                     /translation="MKEKLLVPALNCLLGLLFLGDHWFLFRLQNGELFAIVTLILLCT
                     ASFLINKNHPKNLTLIAMVITFLAVLSFLSTFLLA"
     gene            7842..8909
                     /gene="pepA"
                     /locus_tag="VBD27_001194"
     CDS             7842..8909
                     /gene="pepA"
                     /locus_tag="VBD27_001194"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEO7025206.1"
                     /translation="MSDLFNKIKTVTELDGIAGYEHNIRNFLRQEITPLVDQVETDGL
                     GGIFGVKNTHETNAPKVMVAAHMDEVGFMVSHIQPDGTFRVLEVGGWNPLVVSSQRFT
                     LYTRSGDAIPVISGSVPPHFLRGQSGGTTLPKISDIVFDGGFTDKNEAESFGIAPGDI
                     IVPKSETILTANQKHIMSKAWDNRYGVLMVTELLKSLKDQSLSNTLIAGANVQEEVGL
                     RGAHVSTTKFNPDIFLAVDCSPAGDIYGEQGKIGEGTLIRFYDPGHIMLKDMRDFLLT
                     TAEEAGIKYQYYAAKGGTDAGAAHLKNSGIPSTTIGVCARYIHSHQTLYAMDDFLQAQ
                     AYLQAIVNKLDRSTVDIIKGY"
     gene            8979..9749
                     /locus_tag="VBD27_001195"
     CDS             8979..9749
                     /locus_tag="VBD27_001195"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO7025207.1"
                     /translation="MKIGIIGVGKMASAIIQGLKQTQHDIVISGSCLERSKEIAERLD
                     VTYAESHQSLINQADIIMLGIKPQLFEKVLLPLDITKPIISMAAGISLARLSQLTRSD
                     LPLIRIMPNINAQILQSCTAICYNNHVSDELRQLAKEITDSFGSSFDIAEINFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKYGFPKEQALSIVGQTVLASSQNLLQGQNSTSDLIDN
                     ICSPGGTTIAGLLDLEKNGLTHSVISAIDATIEKAKKL"
     gene            complement(9770..10435)
                     /locus_tag="VBD27_001196"
     CDS             complement(9770..10435)
                     /locus_tag="VBD27_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7025208.1"
                     /translation="MSFIIKKGKILALLIAFLVINQLVPILAVWLLKNHYQTPFTSIL
                     LIGLELLIIALFLYYAKVKQIIRWKVLLTRKALVTILLGWLSLRVPQIIGYLIMTMQG
                     VKDTANQTVIMELTKQLPLALMLIFAIIGAPIMEEIIFRYIIPKELFAKHQKWGFVIG
                     TLAFALIHSPSDIGSFIIYAGMGAILSFVYYKTEHLEYSIMIHFINNALAYSVLISTM
                     MNN"
     gene            complement(10504..10755)
                     /locus_tag="VBD27_001197"
     CDS             complement(10504..10755)
                     /locus_tag="VBD27_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025209.1"
                     /translation="MVITFSEKRFQHYYTKVFDERNQFIANMSCRLLFLLSVLVLVVL
                     CLINVWFGITFSYAKFLLLILSFVLYGYLLIYFILQKLL"
BASE COUNT         3616 a         2155 c         1702 g         3398 t
ORIGIN      
        1 ttttatcatt aataacttaa agttaggtaa aacacaattc tagactctag ctcataaacc
       61 atgttaatcc aatgtatttt aatagacatc ataaaaaggc tgaataaatt cagccttaac
      121 cacgctatta gttatagcaa accaatcaaa tgactaaaga taggaataac caaatcaact
      181 accaaaccga taaccaccac cgctatagct gccatcgcac cttgcagttc tcccaattcc
      241 aatgcaaagg ctgctccgac tgtatgacca gaggtcccta gacctaatcc tgcaccaatc
      301 ttactatcat tcaaatggaa aaatgacact aattttttac ctaatgcata aataataact
      361 gcatttaaga tacacgccat tgctgtcaca gctgtgttcc ctccaattgc tgctgttatt
      421 ggaagagcaa ttgctgttgt tgctgcttgt ggcaacattg aagcaattcc gacttgacta
      481 agcccaacca ttttagaaat agcgacgata agcataaggg aaacaatcat accgattact
      541 aaactgctga gaatttctac ccaatatttt ttaacaacgt catttttctt atagagagga
      601 actgcaaaag caattgttgc tggatttaaa aaccaaaata ttaaatcgcc acctggttta
      661 taagcttgtg tataaaatgt cttaacatcg gttcctaaac cttttgacat aagccaaaga
      721 atgacaatac cactaaccat tgcaaagaaa agaggctgca aaaggaagaa acctttactc
      781 ttcttaaata aatgttgtcc tatcgtatag agtattaaag aaaagcaaat accaaagatg
      841 ggtgttttta agagttccat attatttttc ctctactttc ttactatagt ggttaggtaa
      901 tatcttttta aataaatctg tgacactgag ttctctgata gcaagtatta atcgtgcaac
      961 gtatgctacc actactaaca taacaacagt tgagattgta ataacagcaa ctagttgcaa
     1021 gccttcagct tttaggatat caaggttcgc tgctactgat attcctgagg ggacaaacat
     1081 aaaaccaatc atgcttatca tcaaagcccc aaaagagtca acccactcta ccttgataat
     1141 tttagcagtt agcaatacgt acattagcac caaaccaata accgtcgttg ggattggtag
     1201 tgatttcggt aataacattg aaatcatttg tgaaactaat agaatagctg catatattga
     1261 catttgaata aacattggag cttcttttgt tttggtttct ttactggtgt gtttcatagt
     1321 cactacccct tccttttatt acaatgtttt aacttgtttt attgatagct atattgtact
     1381 tcaaaataaa taaaacgttt tcattttcag cctcagttac caaaaaaaga agccgaattg
     1441 caaattcaag cttctaaaca acaaagggaa gttcgcaaaa gcacttttac atggttaaat
     1501 gctcatttag gtcttttaaa tacgatcgac ctactggaaa tttctctcca ttagacataa
     1561 ttagtaacaa agtatgatta aaccaaggtt gaatttcctc aatcatctct agattaatca
     1621 ctgtgtttcg atgaatctgg agaaaatatg gtgctacagc gcgtgactta aaccaattga
     1681 gtgttttccg aatagtgtaa gttttctgaa ctgtactaac agttaattcg ccatcttcaa
     1741 cactagctgc aacgatatct tgcattttaa caacaacact gcgatctgat aatgtcaagg
     1801 ttaacaattc agcactggct tttttaggta ttgcttgctc tacatctgaa gcagtggtga
     1861 gttggctcaa tttcttcact tttgatagag ccatatcaac gcgctgttgt tcaaacggtt
     1921 tcatgatata atcaacagca ttactttcaa aagctttcac agcgtagtta tcataagctg
     1981 tcgcaaaaac aacaagtggg ggatgtgcaa gttggctcaa ctgattagcc aaagtaaagc
     2041 cattttcttc actaagcgaa atatctaaaa aaattaaatc aatttgctgt ctaaataaga
     2101 ttttttcggc ttcactgata tcctcagcct gaaaaatctc agggttgtca acctcctggc
     2161 tatgttcaac taaaaacgat aattcttgtc tggcaaacat ttcatcatca agaattaaaa
     2221 ttttcatgct catcctccct tattctatta ggtattcgat accaaacttt tgtaccattc
     2281 ttgtcgctcg aaaaatgaag gcaacttaca ctaccatata ataaattcag cctgttattt
     2341 agattaacta gagcagtacc tgtcccctta ctctctgcaa ctgtttcttg acctaattta
     2401 tcaatgatag tatctgagat tccttgtcca ttgtcactaa cagaaacaca ataataatga
     2461 ccatctggct ttatttgaac caatatatgg ttgtccgtct tacgttcttt gaaagcatgt
     2521 cgaactgcat tctctaccag tacctgtaaa ccaaaaggcg gtaacttcat tttttctggt
     2581 gcactaatat cataagataa ctgatattta tcagggaaac gtaatttttc aacattcata
     2641 taagcatcca catgtgattt ttcttgctca agcgttacct cacgatcctg accaccttgc
     2701 aaacttgttc taaaaaaagt acttaactgc atcagtgcat aacgtgcttt atcagaatca
     2761 atacggatta atgcactaat tgtgttaatg gcattaaaga agaaatgagg gttgatttgt
     2821 gcttgtaaag cctttatctc tgccatactg gctaacttat tttgttcctc tgttatcccc
     2881 attgccagtt gtcctgaaaa tatttgcgct aaaccaagga ctaggttttc ctccacctca
     2941 gacattgtct tatctcctgc aaagtacatt tttaaggcac acacagtttt atcatttatt
     3001 tttagaggaa ctacaatagc agaatttaac tgacagttgt gatctggaca agaaatcgcc
     3061 gctttatctt gcgcaattct tggttcgcca tcaaaaataa cacttttaga taagtctgtt
     3121 ttgaccggtt gtcctgcaat atggtgatca tggccaacac caatatgagc taatacgttt
     3181 gaccgatctg ttaatcccac agcatcaaag ttagtatgcc tctttataat ttcgcaaacg
     3241 ctcctagcag attgcggtgt caaaccttgt ctaaggtagg gcagagtctg tcgagtcaat
     3301 tcaagaacat ctctcgtttg aactgcgcgt aactgacttt catttgacaa ataagttttc
     3361 aaaatcgcaa ggaaaagtgt ggaacctaaa ctatttaaaa tcatcattgg aatgacaatc
     3421 attttgacaa gttcccatcc tgtaaaaatg ccaacaaata gcatctggat actttcggca
     3481 ataatactaa ttaaaataac ttggcttgtt gaagggtaga gatggttttc cttaagctta
     3541 tcaccaatct ttccgctaac aatgccgact agaactgaac tgacaatata gaaagaacct
     3601 gaaaagcttc cttgaaaaaa gcgatgaact cctccaataa aaccaacaat tgatccaacc
     3661 agaggtccac caaccaaact tgccgttgta ataactaaag tccttgtatt agcaagtgag
     3721 tcagaatggg aaatcgttgt tagaaaaggg cgctcgacca aacttcgatc cccttttatt
     3781 tcaattcctg ttatattaga tataataaca aacaagccga aaatgatgac aaggactacc
     3841 gtttcacgtt tagaccgctc ttcaattaac tgtctgaaat aactattatt taccaataaa
     3901 aaggctaaaa tcataataat tcctagcctt tggaataaca acaccatcaa catatgctta
     3961 tcctcctcaa gataccattt tactccggaa gataaatata gatatcttga taatctgaaa
     4021 taaccttgtc tgccattgat aaatcttgcg gtggataatc aggattggca aagccaaagc
     4081 aatacattcc tgctgcctta gcagctaaac tcccatttct tgtatcttct ataactatac
     4141 aaactttagg gtctacatct aaaagctctg cagctctaag aaaaacatct ggtgctggtt
     4201 tagaagacga tacatcttct cctgtaacca tgtattcaaa gcattctgtc acacccaact
     4261 cttttagatt acgcttaata tctaccattg gagatgagga cgcaactgct aagcgatagc
     4321 catgttggtg taaccaatga atcagttgct gagctccttt aatgggccta acaccatcac
     4381 gcgtcacaat cgcttgtcga cgtctgttca tctcagcaat atactctttt actgttttcg
     4441 gtaagccaaa ctcctctttc atagcttgcc acatgaattc aaaagtagtc cccatgtact
     4501 ggtattgata cgagacgtcc gtatcaatac cctcctcacg taacatttct gtcttattat
     4561 ctaaaaacgt gtactcggaa tcaacaataa cgccgtccat atcaaaaata atgacttttt
     4621 ccatcttaaa cctgctttct atccccttta ttgtaatcgt ttgcctttct attttcaata
     4681 aaaaaaggtt gctttctgtg gaaagcaacc agtatattta gaaatgatat tttgagagtg
     4741 attttattta aaaggggagc tcctcttctt ccaatactaa atctgacaaa tcaccagaaa
     4801 cattattttc acgcatagca cgttgggcac ggctttctaa caactgaaat gatgatgcta
     4861 atacttcagt gatatagtgc gtttgaccat ccttttcgta cttgcgcgta cgcaattcac
     4921 catctattga aattaaagag ccctttgtcc catagctcgc aagggtttcc gctagacgac
     4981 cccacataac cacattaata aaatctgctt cacgctcacc attacttcct ttaaagcgtc
     5041 tattaacagc aacagttgca cgcgttactg acttgtcagt tggtgttttt accatctcag
     5101 gctttgctgt tagacgccca atcataataa ctttattata cattgattac ctcctcacta
     5161 ttattattcg aaaataagga agaaaaatgc taaaatatat gaaattgttc tgggagaatt
     5221 aagatgacaa atgtaactag ttataaagaa atgctggcta aaccttgggg aaaaatacaa
     5281 tatgaaataa cttttgctca attaagccat atcaaaaatc agaacgtttt agattttgga
     5341 gctggttttt gtctcaccgc acaacatcta gcaaaagaaa acaatgtaac tgctattgaa
     5401 cccaatccta aactgcttta cgataatcaa agtgacaata tttataaaat cctaggttcc
     5461 tacgaagcat taagagcttt gcctgatcaa agttttgata ctattatttg tcataatgtt
     5521 cttgaatata tagacaaaca taatcatcct gcttattttg acgaattttc tcgacttcta
     5581 aaacctaatg gagaactatc tctcatcaaa cataatataa ttggtaaaat attacaatca
     5641 gttattttta gcaatgatac ctccactgcg atggaactct taacaggaga agcaaacttt
     5701 aaaagtgcca gttttgacca gggaaatatt tataccttag aagaactaaa acaaaataca
     5761 aatttattag ttgaacgtta tcagggtatt cgtaccttct attcattaca accaaaccat
     5821 tttaaaactg aaactgattg gctaaacaaa atgcttgcta tcgagctaag tgttgctgac
     5881 aaagctcctt ataaagatat tgcttttttg caacacatca cacttaaaaa gtcattgtaa
     5941 cttcttaagt ttacaactaa aaatcaactt agctgaagct aagttgattt ttactaattt
     6001 ttccaatgtt tattggcgtc gaaagactct ccaacaatta cctgatcaga taattcaatg
     6061 atacctctga cttgaggagc atttggaaga gctaactcgc gagcactaca taacattcca
     6121 aagctatctt ctccacgaag ttttcctggg aaaataaggc taccatttgg catcatagct
     6181 cctggaagag ctgcaactgt tttaaggcct actgaagcat taggtgcccc acaaacgatt
     6241 tgaaccgtct tcccatcgtt gatttctact tgacagatgt gtaaatggtc actatctgga
     6301 tgatcaatca tttctttaat ttgcgccaca acaaatgtag gttcaatatt attaacaaga
     6361 gagtcttcga atccttcttt cagtagttct gcattaagga ttttaataac ttgatcagat
     6421 aaagttactt gacctactcc ctcaatagca attaaacttg acacttcaaa gatattccaa
     6481 gcaactgttt ctttactatc ttgcagatag acgcgtgcta cttgtccgcg acgatcaaca
     6541 tctagcttag ctccttgact atccttaaca ataaccatta aggtatcacc aacatgctct
     6601 ctattataag taaaaatcat ttattctcct ttaaattttg ctattttttg tcttatttat
     6661 ggttaatttc agcgagaaat ttagtaattt cagcctttgt tttgcgattc ttattaacca
     6721 aacgacccag ttcctgacca ttttccacaa caacgaaact cggaatccca aaaatattcc
     6781 actgctgagc taattctata tagtcatcac gattaacacg tacaaagaca aaatctgaga
     6841 aatctttttc aattgatggc attactggat atataaattg acaatctgga caccaatctg
     6901 ctgtaaaaaa gaaaacaact ttcttagtgc tatcaatata agctgctatt tcttcataag
     6961 attctggtaa aatcatttgc tcacctccac ataatcaaga acaccttgac gataaatgaa
     7021 ttcgaaaatt ctgccatctt ccaagacaag accaccactg gtcattttaa tatcagattc
     7081 aaattcatta aaataaaata cctgaatatc acccatctga ctaaaaagtt ctcttaaggt
     7141 ctgagtgatt tgctgacgtt tataatctgt tactttttta gttagggtat aaccagcagc
     7201 tgccagtaaa cccacaccag caaggccagt cccaaataag atttttttat ttttcataac
     7261 tgttattata ccataccccc cggaattaga cacaggaatt gatggaagaa atccacataa
     7321 cagttatgaa aaaaaacaac ttattccttg tggtataata ggatgacatt gtcttaagat
     7381 accattatgg ttaatcattc aaacagaaag aaaaaacatg aaagaaaaat tattagtacc
     7441 tgctttaaat tgtttgctag gtctactctt cctaggtgat cactggtttt tatttcgtct
     7501 tcaaaatgga gaactttttg ctattgtgac gctaatactg ctttgcacag cttcattttt
     7561 aatcaataag aatcacccca aaaatttgac tttaattgct atggttatca cttttttagc
     7621 cgtattatct ttcctatcta cctttttatt agcttaagcc gataaacatt atctccgttg
     7681 tattcaaccc agttttcact ataacaggct ttgtcagtca taacagtctt ctggtagtac
     7741 ttgatattct ttttgaaagt ctttatattg actgaaacct gttctgttct ttatttttaa
     7801 ttaaaaggtg ctaaaatagt atttgtagaa ttgaggtaat tatgtcagat ttatttaaca
     7861 aaattaaaac cgtaactgag cttgatggga ttgctggcta tgaacacaat atccgcaact
     7921 tccttcgtca agaaataact cctttagttg atcaagttga gacagacgga cttggtggaa
     7981 tttttggagt aaaaaatact catgagacta atgctcctaa agtcatggtt gctgcccata
     8041 tggatgaagt cggctttatg gttagtcata ttcagccaga tggaacattt cgtgtacttg
     8101 aggttggagg atggaatccc ctagtagtca gctcacaacg ctttaccctc tacacacgtt
     8161 ctggtgatgc cattcctgtt atatcaggct cagttcctcc tcactttctt cgtggacaaa
     8221 gcggtggaac aacattaccc aaaattagtg acattgtttt tgatggagga ttcacagata
     8281 aaaatgaagc tgaaagcttt ggcattgctc ctggcgatat cattgttcct aaatctgaaa
     8341 ccattttaac tgcaaatcaa aaacatatta tgtcaaaagc ttgggataat cgctatggtg
     8401 tgcttatggt gaccgaattg ctaaaaagct taaaagatca aagtcttagc aacacactta
     8461 ttgctggggc taatgttcaa gaagaagtcg gacttcgtgg cgcacatgtt tcaacaacta
     8521 aattcaaccc agatatcttc ttagctgtcg attgttcccc agctggagat atttatgggg
     8581 aacaaggcaa aataggagag ggaaccttaa tccgttttta tgatcccgga catatcatgc
     8641 ttaaagatat gagagatttc ttgcttacaa cagctgaaga agcaggtata aaataccaat
     8701 attatgctgc aaaaggtggt accgatgctg gggctgctca cctaaaaaat agtggtattc
     8761 cttctacaac tatcggtgtc tgtgcacgct acattcattc tcatcaaaca ctctacgcta
     8821 tggatgattt tctacaagca caagcttacc ttcaggccat cgttaacaaa ttagaccgct
     8881 cgacggtgga tattattaaa ggttattaag atagaaagga tgggagtttc ataaggtaga
     8941 cacattttag tatctccttt catccccata tcaacattat gaaaattgga attatcggcg
     9001 ttggaaaaat ggccagtgca attattcaag gcctcaaaca aacccaacat gacattgtca
     9061 tatccggatc ttgcttagag cgttcaaaag aaattgcaga aagacttgat gttacttacg
     9121 ccgaatctca tcagtcatta atcaatcaag ctgatattat catgttaggt attaaacctc
     9181 aactatttga aaaggttctt cttccacttg atatcacaaa accaatcatc tcaatggcag
     9241 caggaatctc cttagcgcgt ttaagccaat taactcgttc tgatttacca ttgattcgta
     9301 ttatgccaaa tattaacgca caaatacttc aaagttgcac agctatttgt tacaacaatc
     9361 atgtatcaga tgagttacga caattagcca aagaaattac ggatagtttt ggaagtagct
     9421 ttgatatagc agaaatcaat tttgatacct tcactgctct agcaggttct agtcctgcct
     9481 atatctatct atttatagaa gccttagcta aagcaggtgt aaaatatgga tttccaaaag
     9541 aacaagcact ctctattgta ggacagactg tcttagcttc tagtcaaaac ctattacaag
     9601 gacaaaatag cacctctgac ttaatcgaca acatttgtag cccaggcgga actactattg
     9661 ctggcctttt agacttagaa aaaaatggtc tgacgcatag tgttatttct gccattgatg
     9721 ctactattga gaaagcaaaa aaactttgag caaattgctc aaagtttttt tagttattca
     9781 tcattgtact gattaacacg ctataagcaa gagcattgtt aataaaatga atcattatac
     9841 tatattctaa gtgctcagtt ttataataaa caaaagataa aatagcaccc atcccagcat
     9901 aaataataaa actacctata tcagaagggg aatgaataag agcaaaagct aaagtaccga
     9961 tgacaaaacc ccatttttga tgtttcgcaa aaagttcttt agggataata tagcgaaata
    10021 taatttcttc cataattggt gcccctatta tggcaaaaat taacatcaaa gctagtggaa
    10081 gttgctttgt taattccata attactgttt gatttgctgt atctttaacc ccttgcatag
    10141 tcataatgag gtaaccaata atttgtggaa ccctcaatga taaccaacct aagagaatag
    10201 taactaatgc tttccttgtc aataatactt tccatctgat tatttgttta accttggcat
    10261 aataaaggaa aagagcaata ataagtaatt ctaagccaat taacaatata ctagtaaaag
    10321 gcgtttgata gtgattcttt aggagccaca cagccaatat aggaacaagt tgattaataa
    10381 ctaaaaatgc tattagcaaa gctaaaattt tgcctttttt aataataaat gacacaaaaa
    10441 cctccaaaat aaattattaa cttttttcta tcataacata ttttcaaatc ttatagattt
    10501 atctcacaat aacttctgta aaataaagta aattaataaa taaccgtata aaacaaaaga
    10561 gagtatcaat agcaagaact ttgcatagga aaaagttata ccaaaccaga cattaatcag
    10621 acacaatacc actaaaacaa ggacagataa aaggaaaagg agtcgacaag acatattagc
    10681 aataaattga ttacgctcat caaaaacttt ggtatagtaa tgttgaaatc gcttttcact
    10741 aaatgttatc accaaaaaat aaaaacttcc tgcaaataag gcaatacctg aaccaaagga
    10801 aaaaccttta gtaaaattag attcaaaggt atgtagaaat gatgatacta tcataatcaa
    10861 actacataca c
//
LOCUS       DATRQA010000062        10759 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.062, whole genome shotgun sequence.
ACCESSION   DATRQA010000062 DATRQA010000000
VERSION     DATRQA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.062"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            203..622
                     /locus_tag="VBD27_001198"
     CDS             203..622
                     /locus_tag="VBD27_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO7025210.1"
                     /translation="MDNCIFCKIISGEISSSKVYEDDEVLAFLDITQTTTGHTLLIPK
                     KHVRNVLEMDEKTAQITFERLPKVARAVQAATKAKGMNIINNNEEIAGQTVFHAHVHL
                     VPRFDESDGIKIHYTTHEPDFEALAKLAKEIRKEIQL"
     gene            619..915
                     /locus_tag="VBD27_001199"
     CDS             619..915
                     /locus_tag="VBD27_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025211.1"
                     /translation="MKIGRLIALGLVSLGAFELYKNRKTIKDSYQNTKNETDSAKLKL
                     ERIKNDLAIISQEKEKIRLISQELNHKFQVFNKDIQPRLEEINQRMAKYQEKDE"
     gene            complement(979..2286)
                     /locus_tag="VBD27_001200"
     CDS             complement(979..2286)
                     /locus_tag="VBD27_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7025212.1"
                     /translation="MKIWKKITLMFSAIILTTVIALGVYVASAYNFSTNELSKTFKDF
                     KLAKSKSHAIEETKPFSILLMGVDTGSEHRKSKWSGNSDSMILVTINPKTNKTTMTSL
                     ERDVLIKLSGPKNNGQTGVEAKLNAAYASGGAEMALMTVQDLLDINVDYFMQINMQGL
                     VDLVNAVGGITVTNKFDFPISIAANEPEYKAVVEPGTHKINGEQALVYSRMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALNSISSYKKILSAVSNNMQTNIEISSKTIPNLLAYKD
                     SLEHIKSYQLKGEDATLSDGGSYQILTKKHLLAVQNRIKKELDKKRSKTLKTSAILYE
                     DYYGTTASNDSSTYSSTQENNYNTTPYSEAPPSYSGNTTYSSETNQTTHQSYYNSSTP
                     ASNYSSNTNTGQADSSGSVNNYNGAATPNPNTGTQPVPSQTNP"
     gene            complement(2295..2855)
                     /locus_tag="VBD27_001201"
     CDS             complement(2295..2855)
                     /locus_tag="VBD27_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7025213.1"
                     /translation="MGIAQEKLLRRFKMSKQFAVDFIEASREEASALLEFLNTVTEET
                     DFILHTVSNQLSLPEMETFIENTLITKNCICLIAKLKNKVIGLITIISQSDIEIEHIG
                     DLFIAVQKDYWGYGIGHILMEEAIEWASDNDITRRLELSVQGRNERAIHLYQKFSFEI
                     DGLQTRGIKRENGEFLDIYRMSKLID"
     gene            complement(2809..3318)
                     /gene="tsaE"
                     /locus_tag="VBD27_001202"
     CDS             complement(2809..3318)
                     /gene="tsaE"
                     /locus_tag="VBD27_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO7025214.1"
                     /translation="MLWGCQNQQTVGRQRYFWYNISMFYTQNEEELIALGQKLGTVLK
                     SGDIVLLTGNLGAGKTTLTKGIAKGLDIKQMIKSPTYTIVREYEGRVPLYHLDVYRIG
                     DDPDSIDLDDFLFGQGVTVIEWGELLSDNLINNYLEIVITRSNQGRQVQLEAYGHRAR
                     EIIEAIQDV"
     gene            complement(3401..4822)
                     /locus_tag="VBD27_001203"
     CDS             complement(3401..4822)
                     /locus_tag="VBD27_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO7025215.1"
                     /translation="MEKFFKLKEHGTTIRTEITAGLTTFFAMSYILFVNPAILSQTGM
                     PAQGVFLATIIGAVVATSVMAFYANLPYAQAPGMGLNAFFTYTVVFALGYTWQEALAM
                     VFICGLISLIITLTKVRKMIIESIPTTLKSAITAGIGTFLAYVGIKNAGFLKFSIDPG
                     TYDVVGKGAAKGLATITANSSATPGLVSFDNPAILLSLIGLSITIFFIVKGIRGGIIL
                     SILTTTLLGILMGVVKLDAINWEATNLSASFRDLKQVFGVALGEKGLISLFSNPSRLP
                     SVLMAILAFSLTDIFDTIGTLIGTGEKVGILATTGDNHESKSLDKALYSDLIGTTFGA
                     ICGTSNVTTYVESAAGIGAGGRTGLTALVVAGLFAISSFFSPLVSIVPSQATAPILVI
                     VGIMMLSNLKDIKWDDMSEAIPAFFTSLFMGFTYSITYGIAAGFLTYTLAKVIKGQAK
                     DIHVVLWILDILFILNFISLAIL"
     gene            complement(4925..5536)
                     /locus_tag="VBD27_001204"
     CDS             complement(4925..5536)
                     /locus_tag="VBD27_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO7025216.1"
                     /translation="MKVKLFAIHLFFIVISFGIKYYHLQGPNLIWNMTLALIALDFAY
                     LTSLFKKKILIGLFALAWFFFYPNTFYMLTDIIHMHFVGDVLYNKTNLILYILYVSSI
                     LFGFLSGIESFSVIMRKFRISNIFLRWGIIGIVSFVSSFGIHIGRYARLNSWDILTKP
                     QVVINELLAVPSRDSFHFILGFTFLQVLCLIFMDYSAGKKLEK"
     gene            complement(5626..6210)
                     /locus_tag="VBD27_001205"
     CDS             complement(5626..6210)
                     /locus_tag="VBD27_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025217.1"
                     /translation="MKKLIWSHLFAIVSILGIWYYHVKIDHLMVILLASVLSLEMGFI
                     AYYSRNIFIRLLASLGLLFFYPQSLGIFLSLVSLEWSTKDIWQSNHFMQYMAYLLAIL
                     FIISSALLSLKLLVNAYDISKRYQLATVIPVMLFSSFAFCLEKSGSLGLEGIILNPSA
                     FFNGIQTNMANLDFKALSCLFLVQLLLWGILMED"
     gene            6299..7111
                     /locus_tag="VBD27_001206"
     CDS             6299..7111
                     /locus_tag="VBD27_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7025218.1"
                     /translation="MTKKIIAVDLDGTLLHNNNTISDYTADTLRKVQAQGHKVIITTG
                     RPYRMALAHYLRLDLKTPMINFNGALTHIPEKKWAFEHSATIDKKLLLETLNLSDAIQ
                     ADFIASEYRKNFYITMDNRDKINPQLFGVNEITDKMALDVTKITRNPNALLMQTRHKD
                     KYELAKELRQHFNHELEVDSWGGPLNILEFSPKGVNKAYALKHLLKSLNLSQENLIAF
                     GDEHNDTEMLAFAHTGYAMKNASPTLLPYADQQIQWTNEEDGVAKTLEKLLL"
     gene            7413..8423
                     /gene="manX"
                     /locus_tag="VBD27_001207"
     CDS             7413..8423
                     /gene="manX"
                     /locus_tag="VBD27_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEO7025219.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIAQFDADDEVLVLADLWSGSPFNQASRVMGENPERKMAIITGLNLPMLIQ
                     AYTERMMDANAGVEQVAANIIKESKEGIKALPEELNPVVEATPVAGVPADVPAEVKQS
                     GSIPEGTVIGDGKLKINLARIDTRLLHGQVATAWTPASKANRIIVASDEVSKDELRKQ
                     LIKQAAPGGVKANVVPISKLIEVAKDPRFGNTRALILFETVQDALRAVEGGVEIPELN
                     VGSMAHSTGKTMVNNVLSMDKDDVAAFEKLRDLGVSFDVRKVPNDAKKNLFDLINKAN
                     VK"
     gene            8456..9268
                     /locus_tag="VBD27_001208"
     CDS             8456..9268
                     /locus_tag="VBD27_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEO7025220.1"
                     /translation="MADISIISMILVVIVAFFAGLEGILDQFQIHQPLVACTLIGLVT
                     GHLEAGIILGGSLQMIALAWANIGAAVAPDAALASVAAAIILVKSGDFTQKGINFAFS
                     TAVPLAIAGLFLTMIVRTISTALVHAGDKAASEGNFAAIERFHFIALLLQGLRIAFPA
                     ALLLAIPSSSVQSILEAMPDWLNGGMQVGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAALNQLTLIAMGTIGVAIALIYISLSKMGGSKGTSNAGSNDPIGDILEDY"
     gene            9283..10194
                     /locus_tag="VBD27_001209"
     CDS             9283..10194
                     /locus_tag="VBD27_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7025221.1"
                     /translation="MTEQIKLSKSDRQKVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTTKEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEEKASGTPVEDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASAGNILGPIIFFVGWNLIRMSFLWYTQE
                     LGYKSGKEITKDMSGGILQDITKGASILGMFILAVLVKRWVAINFTVDLPKKTLSEGA
                     YINFPKDHVSGQQLHDILGQVQSGLSLDKMQPQTLQGQLDSLIPGLAGLLLTFFCMWL
                     LKKKVSPITIIIGLFIVGILARLAGVM"
     gene            10313..10675
                     /locus_tag="VBD27_001210"
     CDS             10313..10675
                     /locus_tag="VBD27_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEO7025222.1"
                     /translation="MAQSLNKTVEFQTTGVSYLGMGNKVGKFLVGDQALEFYNDKNVN
                     DYIQIPWTSINQIGANVSRKKISRHFEVFTDQGKFLFASKDSGTILKHARRHIGDDKV
                     VKLPTLIQTILKIFKFKK"
BASE COUNT         3618 a         2011 c         1740 g         3390 t
ORIGIN      
        1 tgtacttact caataataaa catcattaaa aattgcaata ccctttaggt catgctaaga
       61 taagtatgat ggtaaatata gatatcacaa tcaatacagg ctacttttat ccacctgtac
      121 gacttatgac ctatcaaacg ataaaaattt accattcctt aatagctacc caataaattt
      181 ttcaacatga aaggaattag ttatggataa ttgtattttt tgtaaaatca tcagtggaga
      241 aatctcttca tcaaaagttt atgaagatga tgaagtatta gcttttctcg acattacaca
      301 aactacgact gggcacaccc ttcttattcc taaaaaacat gttcgaaatg tgttagagat
      361 ggatgaaaaa actgcacaga taacctttga acgcttgcct aaagtggcgc gtgctgtcca
      421 agctgctaca aaagccaaag gaatgaatat tattaacaac aatgaggaaa ttgctggaca
      481 aactgttttt catgcacacg tccaccttgt tccccgcttt gatgaatcag acggtatcaa
      541 aattcactac actactcatg aacccgactt tgaggcactt gcaaaattag ccaaagaaat
      601 tagaaaagag attcaattat gaaaataggt cgtttaattg cactaggttt agttagttta
      661 ggagcgtttg aactttacaa aaatagaaaa actatcaagg attcctatca aaatacaaaa
      721 aatgagacag attcagctaa attaaaatta gaacgtatca aaaatgatct tgctattatt
      781 agtcaggaaa aagaaaaaat cagattaatt tctcaagaac tcaatcataa attccaagtg
      841 tttaataaag atattcaacc tcgtttagaa gaaattaatc aacgcatggc taaatatcaa
      901 gagaaagacg aataagccga cggtataaaa atatctactc taaatataaa aagagcaaat
      961 aatgctcttt tttatatttt atggattagt ttgacttggt actggttgcg ttcctgtgtt
     1021 tggattaggc gttgcagccc cgttataatt attaacactt ccacttgaat cagcctgacc
     1081 tgtgttagtg ttactgctat agttactagc aggagtgcta ctattatagt aactttgatg
     1141 agttgtttga ttagtctcag aactataagt agtattacca ctgtaacttg gtggtgcttc
     1201 tgaataaggt gttgtattat aattattctc ttgtgttgat gaataagtag aagaatcatt
     1261 actagcagta gtaccatagt aatcttcata tagaatcgcg cttgtcttca gagttttact
     1321 acgcttttta tccagctctt tcttaattct attttgaact gcaagtagat gtttcttagt
     1381 taaaatttga taagagccac catctgatag agtagcgtct tcacccttca actgataaga
     1441 tttaatatgt tccaatgaat ctttataagc taacaaatta ggaatcgttt ttgatgatat
     1501 ctcaatatta gtttgcatgt tattacttac tgcggaaaga atttttttgt atgaactaat
     1561 actatttaac gccaatattt ttttaaggac tttttgaatt acttcacgtt gtcttttttg
     1621 acgcccataa tctccctctg gatcatcata gcgcatacga gaataaacaa gtgcttgttc
     1681 tccatttatt ttatgtgtcc ctggttcaac aacagccttg tactctggtt cattggcagc
     1741 aattgatatt ggaaagtcaa atttattagt tactgttata ccaccaacag cattgaccaa
     1801 atcaactaat ccttgcatat taatttgcat aaagtaatca acattaatat ctaataagtc
     1861 ttgaacagtc atcaatgcca tttccgcacc accagaagca taggctgcat ttagctttgc
     1921 ttctacgcca gtctgtccat tatttttggg accactcaat ttaatcaata cgtcacgttc
     1981 taagcttgtc atcgttgttt tattagtttt aggatttata gtgactaaga tcatagaatc
     2041 gctatttcct gaccacttag attttcgatg ctctgaacct gtgtccacac ccattaatag
     2101 tattgaaaac ggctttgttt cttcaatagc atgacttttt gatttagcta gcttaaaatc
     2161 tttaaaagtc ttagacaatt cattagtcga aaaattataa gctgaggcaa catagactcc
     2221 caatgcaatt actgttgtta aaataattgc agaaaacatt aaggttattt ttttccaaat
     2281 tttcatgtat attcctaatc tatcaactta ctcattctgt aaatgtctag aaattctcca
     2341 ttttctcttt taatacctct tgtttgaagc ccatctattt caaaactgaa tttttggtag
     2401 aggtgaatag cacgttcatt acgtccttgg actgatagtt ctaagcggcg tgtaatatcg
     2461 ttatctgacg cccattctat tgcttcttcc atgagaatat gaccaatacc atacccccaa
     2521 taatccttct gaacagcaat aaaaagatcc ccaatgtgtt caatttcaat atcagactga
     2581 ctaatgattg ttatcaagcc gatcacttta ttttttaatt tagcaatcaa acaaatgcaa
     2641 tttttcgtta ttaaggtatt ttcaataaac gtttccattt ctggcaaaga tagttgattg
     2701 ctcaccgtat gcaaaataaa atctgtttcc tcagtaacag tatttaaaaa ttcaagaagc
     2761 gcagatgctt cctctctcga agcttctata aaatcaacag caaattgttt agacatcttg
     2821 aatcgcctca ataatttctc ttgcgcgatg cccataagct tctaactgaa cttgacgtcc
     2881 ttgattgctc ctcgtaataa caatttctaa gtagttattg ataaggttat cactaagcaa
     2941 ctctccccat tcaataactg ttactccttg accaaacaag aagtcatcta agtcaataga
     3001 atcaggatca tctccaatac gataaacatc caaatggtaa agtggtacac gcccttcata
     3061 ttctctaaca atagtgtaag ttgggctctt aatcatttgt tttatgtcta atccctttgc
     3121 aatacctttt gtaagagttg ttttgcctgc acctaaatta ccagtcaata atacgatatc
     3181 tcctgacttt aaaacagtac ccagtttttg ccctagggca ataagctctt cttcattttg
     3241 agtataaaac atagagatat tataccagaa atatctctgt cttccaacag tttgctgatt
     3301 ctgacagccc catagcatat atttcttcac ttttcttaag aaaaaaggaa accaggctac
     3361 aaatctgatt tccttttatc ttatttatca aactataata ttacaaaata gccaagctga
     3421 taaagtttag aataaataat atatctaaaa tccacagtac cacatgaata tcttttgctt
     3481 gtcctttgat gactttagct agtgtatagg tcaagaaacc agctgcaata ccataagtaa
     3541 tgctataagt aaaccccata aaaagagaag taaagaaagc tgggatagct tctgacatat
     3601 catcccattt gatatctttt aaatttgaaa gcatcataat accaacaata accaatattg
     3661 gtgcagtagc ttgactcgga acgatagata ctagtggact aaaaaaacta gatatagcaa
     3721 acaaaccagc aactactaag gctgtcaatc ctgtacgacc accagctccg attcctgctg
     3781 ctgactcaac ataggttgta acgttagatg ttccacaaat agcaccaaaa gttgtcccaa
     3841 ttaagtctga atagagtgct ttgtctaaac tcttagattc atgattatca ccagtcgttg
     3901 ctaagatacc aactttttct ccggtaccaa tgagcgttcc aatggtatca aaaatatcag
     3961 tcagtgaaaa agcaagaatg gccattaata cacttggtag acgtgaagga ttagaaaata
     4021 aagaaattaa tcctttctct cctaatgcaa ctccaaatac ttgcttcaaa tctctaaaag
     4081 aggccgagag gttagttgct tcccaattaa tagcatctaa cttaaccact cccattaaaa
     4141 tacctaagag ggtcgttgtt aatatcgata gaataatccc tccacggata ccttttacaa
     4201 taaagaagat ggtaatgctt aaaccaatca aagataataa aattgctgga ttatcaaacg
     4261 acactagccc tggcgttgct gaagaatttg ctgtaatagt agccaatcct ttcgcagcac
     4321 cctttccaac aacatcatac gtcccaggat caattgaaaa tttcaaaaaa ccagcatttt
     4381 taatcccaac gtatgctaaa aaagttccga ttcctgctgt aatggctgat tttaaagttg
     4441 ttgggataga ctcaataatc atctttctaa ctttcgttaa agtaataatc agagaaatca
     4501 aaccacaaat aaataccata gctaaggctt cttgccatgt atatcccaat gcaaagacaa
     4561 ctgtatatgt aaagaaagca tttaatccca tcccaggagc ttgtgcatag ggaaggttag
     4621 catagaaagc cataacagat gtagctacaa cggcaccaat aatcgtcgct aagaaaactc
     4681 cttgtgctgg catcccagtc tgagataaaa tagctggatt tacaaacaaa atgtacgaca
     4741 ttgcaaagaa agtcgttaga ccagcagtga tttcagtccg aattgtcgtc ccgtgttcct
     4801 ttagcttaaa aaacttttcc attttaaaat cggattctcc tttatttttt gttattttac
     4861 acttttaaaa aaattaatcg ttcgtatatt tacgaactat taatatatat taacaaaaaa
     4921 taattcactt ttcaagcttt ttccctgcag aataatccat aaaaataaga cacaatacct
     4981 gtaaaaatgt aaatcctaaa ataaagtgga atgaatccct actcggaacc gctaaaagtt
     5041 cgttaattac cacttgtggc ttcgttaaga tatcccatga atttagacgg gcataacgac
     5101 caatatgaat accaaaactt gaaacaaatg atacaattcc aataattccc catcttaaaa
     5161 atatattgct aattctaaat ttacgcataa tcacagaaaa actttcgata cctgataaaa
     5221 aaccaaacaa gatactagat acatacaaga tataaagtat taaatttgtc ttattataaa
     5281 gaacgtctcc gacaaaatgc atatgaatga tatccgtcaa catataaaag gtattaggat
     5341 agaaaaaaaa ccaagcaagt gcaaaaagac cgataagaat ttttttctta aataaactag
     5401 taaggtatgc aaaatctaac gctattaggg ctagagtcat attccaaatc aaatttggtc
     5461 cctgcaaatg ataatattta atcccaaaag aaataacaat aaaaaataaa tgtatcgcaa
     5521 atagttttac tttcattcta acctccaatt aatatctcat tatacaacat attattaaat
     5581 taaggttaaa taaacttcaa acctaaaaga ctaaccctgt tgtggttagt cttccattaa
     5641 tattccccaa agtaataatt gtactaaaaa aaggcaagac aaggctttaa aatcaaggtt
     5701 agccatatta gtctgtattc cattaaaaaa tgctgacgga tttaaaatga ttccttctaa
     5761 acctaatgat cctgattttt ctaagcaaaa ggcaaaacta ctaaaaagca ttacaggtat
     5821 aacagttgct aattgatacc ttttactaat atcatatgca tttaccaata gcttcaaact
     5881 tagtaaagct gaagagataa taaaaagaat tgcaagaagg tatgccatat attgcataaa
     5941 gtgattagac tgccaaatgt cttttgttga ccactctaag ctcactaaac taagaaaaat
     6001 ccctaaactc tgtgggtaaa aaaataaaag gcctaaactt gctaaaagac gaataaaaat
     6061 attacgacta taataagcaa tgaatcccat ctctaacgac aagactgatg ccaatagaat
     6121 taccatcaaa tgatcaattt ttacatgata ataccatatc cctaaaatac tgactatggc
     6181 aaaaagatga ctccaaatta actttttcat aatccaccaa cttccatatt tccacagtta
     6241 ttatatcata tttctactgt ctctttttat tgaaatctat ttttgatata atgtcagcat
     6301 gacaaagaaa attatcgccg tagatttaga cggtactcta ttacataata ataacactat
     6361 ttctgactac accgctgata cacttcgaaa agttcaagct caaggacaca aagtaattat
     6421 tacaaccgga cgcccatacc gtatggcact cgctcattat ttacgcctag atttaaaaac
     6481 accaatgata aattttaacg gtgctttaac tcatatcccc gaaaaaaagt gggcgtttga
     6541 gcatagtgca acgattgata aaaaattact ccttgaaacc ttaaatttat cagatgctat
     6601 ccaagccgat tttattgcta gcgagtaccg taaaaatttt tacattacta tggataatcg
     6661 tgataaaatt aacccacaat tattcggtgt caatgaaatt actgataaaa tggcattaga
     6721 tgttactaaa atcacacgca atccaaatgc tctattgatg caaacacgtc ataaagataa
     6781 gtatgagtta gctaaagagt tacgacaaca ttttaatcac gaactagaag tagattcttg
     6841 gggtggtcca ttgaatatac tcgaattttc acctaaaggt gttaacaaag cttatgctct
     6901 caaacattta ttaaaatcac tcaatctttc acaagaaaat ttgattgcct ttggagatga
     6961 acacaatgac accgagatgc tggcctttgc acatacagga tatgctatga aaaatgctag
     7021 tccaactcta cttccatatg ccgaccaaca aatccaatgg acaaacgaag aagatggtgt
     7081 tgctaaaact ctagaaaaat tattattgta gtattttcaa aaacgatctc tattgaaaca
     7141 gaattctaat agaaatcgtt ttttaggttt tctactatat tattaacaaa aaaagttacc
     7201 actctcttcc cccattataa aaggtatttg tgaaaaaaat atcttttttt taatttttat
     7261 tagaaatagt attgattttt ttctgaaaac gttttaaaat atatctgttc tataatttcg
     7321 ttgaaatgaa aaagaatggc caattttacg attggaagga gattattctt ctttatgtca
     7381 acgaaaaaat tttttaagga ggaagaacaa gaatgggtat cggtattatt attgccagcc
     7441 atggtaaatt tgctgaaggt attcatcaat caggttcaat gatctttggt gaacaagaaa
     7501 aagttcaagt tgtaactttc atgccaaatg aaggaccaga tgatttgtat ggacacttca
     7561 acaatgctat cgcacaattt gatgctgatg atgaggtgct tgtgttagct gacctttgga
     7621 gtgggtcacc atttaaccaa gcaagccgtg ttatgggaga aaatcctgaa cgtaaaatgg
     7681 ctatcatcac tggtctcaat ttgccaatgc ttatccaagc ctatactgag cgtatgatgg
     7741 acgctaatgc aggtgttgaa caagttgccg caaatattat taaagagtct aaggaaggta
     7801 tcaaagccct tccagaagaa cttaatcctg ttgtagaagc tactcctgta gcaggtgttc
     7861 ctgcagatgt tcctgcggaa gttaaacaaa gtggatctat ccctgaagga actgttattg
     7921 gagatggcaa gcttaaaatt aatcttgctc gtattgacac acgtctttta catggtcaag
     7981 ttgcaactgc atggactcca gcatcaaaag caaaccgtat tatcgttgct tctgatgaag
     8041 tttctaaaga tgaacttcgt aaacaattga ttaagcaagc tgctcctgga ggagtcaaag
     8101 caaacgttgt cccaattagt aaattaattg aagtagctaa agacccacgt ttcggaaaca
     8161 cacgtgcctt gatcttattt gaaactgtac aagatgcact ccgtgcagtt gaaggtggtg
     8221 tggaaatccc tgaattgaac gttggttcaa tggctcactc aactggtaaa acaatggtaa
     8281 acaacgttct ttcaatggat aaagacgatg ttgctgcctt tgaaaaatta cgtgacttag
     8341 gtgtatcatt tgatgtacgt aaagtaccaa atgacgctaa aaagaatttg tttgatttaa
     8401 tcaacaaagc aaacgtaaaa taacttatta tctctgaaaa cgaaaggata tcaaaatggc
     8461 ggatatttca attatctcaa tgattttggt cgttatcgtt gccttctttg ctggtcttga
     8521 aggtatcctc gaccaattcc aaatccatca acctcttgtt gcttgtactt taatcggtct
     8581 tgtaactggt cacctcgaag cgggtattat tcttggtggt agtctccaaa tgattgctct
     8641 tgcttgggca aatatcggag ctgccgtagc accagacgct gcacttgcct ctgttgctgc
     8701 tgctattatt ttggttaaaa gtggcgactt cactcaaaaa ggtatcaact ttgccttctc
     8761 tactgctgta cctctagcta ttgctggact tttccttaca atgattgttc gtacaatctc
     8821 aactgcactt gttcacgctg gtgacaaagc tgcttctgaa ggaaacttcg cagcaattga
     8881 acgttttcac tttattgccc tccttctcca aggattacgt attgcattcc cagctgcgct
     8941 tcttttagct attccatctt cttcagtaca aagtattctt gaagcaatgc ctgattggtt
     9001 aaatggtggt atgcaagttg gtggtgccat ggttgttgcc gttggttacg ctatggttat
     9061 caatatgatg gctactcgcg aagtttggcc tttctttgct cttggatttg ctctagcagc
     9121 tttgaaccag ttaactctta ttgcaatggg tactattggt gttgctatcg ccctcatcta
     9181 catcagttta tcaaaaatgg gtggaagcaa aggtacttct aacgcaggtt ctaacgatcc
     9241 tatcggcgat atcttagagg actactagaa aggagctaaa caatgactga acaaattaaa
     9301 ttatcaaaat cagatcgtca aaaagtttgg tggcgttcac aattccttca aggttcttgg
     9361 aactatgagc gtatgcaaaa tatggggtgg gcctacgctc ttatcccagc attgaaaaaa
     9421 ctttacacta ctaaagaaga tcgtgctgct gcgcttgagc gccatatgga attctttaac
     9481 acacatccat acgttgctgc tccaattatc ggtgtaaccc ttgctcttga ggaagaaaaa
     9541 gcaagcggta cacctgtcga agacaaagca attcaagggg ttaaaatcgg tatgatggga
     9601 cctcttgctg gtattggtga tccagtattc tggtttactg tacgtcctat cttaggtgct
     9661 cttggtgctt cacttgcctc tgctggtaat atcctcggac caatcatctt ctttgttggt
     9721 tggaacctca ttcgtatgtc attcttatgg tatacacaag agttaggtta caaatcaggt
     9781 aaagaaatta ctaaagatat gtctggcggt atcctccaag atattactaa aggtgcttca
     9841 atccttggta tgttcattct tgctgtcctt gttaagcgtt gggtagcaat taactttact
     9901 gtagaccttc ctaaaaagac attgtcagaa ggggcataca tcaatttccc taaagaccat
     9961 gttagtggac aacaattgca tgatatccta ggacaagttc agagtggctt aagccttgat
    10021 aaaatgcaac cacaaacact tcaaggacaa cttgactcac ttattcctgg tttagctgga
    10081 ttacttctta cattcttctg tatgtggtta cttaagaaga aagtatcacc aattacaatt
    10141 atcatcggtt tgtttatcgt tggtattcta gctcgccttg caggtgtgat gtaataaaac
    10201 taagagatgg ttacaatagt attccatctc ttatatttta ttaccaaaaa tccacttaga
    10261 gtcccactat tatgatataa tcaaactact ataaattaaa aagggattag tcatggcaca
    10321 atcattaaac aaaactgttg aatttcaaac aacaggagtt tcgtatttag gtatggggaa
    10381 taaagttgga aaatttttag ttggtgacca ggcattagaa ttctataacg ataaaaatgt
    10441 caacgactat attcaaattc catggacatc aatcaatcaa attggagcta atgttagtcg
    10501 aaaaaaaatt agtcgtcact ttgaggtctt tacagaccaa ggaaaatttt tatttgcctc
    10561 taaagactct ggaacgatat taaagcatgc gagacgacat attggagacg ataaagttgt
    10621 taagcttcct accctcatcc agaccatttt aaaaatattc aagtttaaaa aataagatag
    10681 actatagtct atcttatttt ttaactctaa tattaatatg ggaacttgtc atcaaaccat
    10741 ctcaaaaagt tagaaaaat
//
LOCUS       DATRQA010000063        10482 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.063, whole genome shotgun sequence.
ACCESSION   DATRQA010000063 DATRQA010000000
VERSION     DATRQA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.063"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..290
                     /locus_tag="VBD27_001211"
     CDS             <1..290
                     /locus_tag="VBD27_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025223.1"
                     /translation="LVKKIKFVDEVMDYTLIVDRNCQVKCVPLVQTNSHKELSNSIAF
                     DIRSQDFHYLIGVHMDDIIFGDKLYLMQGIKCKGKVIVYDKNNGKMSRLKN"
     gene            370..1371
                     /locus_tag="VBD27_001212"
     CDS             370..1371
                     /locus_tag="VBD27_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7025224.1"
                     /translation="MSKKMTINDIAQLSKTSKTTVSFFLNQKFEKMSDETRQRIQEVI
                     DETGYRPSTIARSLNSKKTKLLGVLIGDITNTFSNQIVKGIEHITKQKGYQIIVGNSN
                     YDAKSEEDYIENMLNLGVDGFIIQPTSNFRKYSRILKEKKKPMVFFDSQLYEHKTSWV
                     KANNYDAVYDMTQECLNRGYKKFIMITADTSLLSTRIERASGFMDALKDNGFGYDTLV
                     IEDDDHSKSDIEDFLKAVVPDKEETLVFAPNCWALPMVFTAMKNLNFDMPRVGLVGFD
                     NIEWTDFSSPKVSTIVQPAYEEGEQVAQILINRIEGDDSVDNQQIVDCQMFWKESTF"
     gene            complement(1443..2057)
                     /locus_tag="VBD27_001213"
     CDS             complement(1443..2057)
                     /locus_tag="VBD27_001213"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO7025225.1"
                     /translation="MSAIDKLVKASHLIDMNDIIREGNPTLRKVAEEVTFPLSEKEEI
                     LGEKMMQFLKHSQDPIMAEKLGLRGGVGLAAPQLDISKRIIAVLVPNVEDAQGNPPKE
                     AYSLQEVMYNPKVVSHSVQDAALSDGEGCLSVDREVPGYVVRHARVTIEYFDKTGEKH
                     RLKLKGYNSIVVQHEIDHIDGIMFYDRINEKNPFAVKEGLLILE"
     gene            complement(2123..2767)
                     /locus_tag="VBD27_001214"
     CDS             complement(2123..2767)
                     /locus_tag="VBD27_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO7025226.1"
                     /translation="MDIENYCQDYCLETIFLKHYFKDLQVITLTAGQSVCKQGEQLEY
                     LHYIVKGRFKIVRRLFNGKEHILDIKTKPTLIGDIELLTNRQIVSSVIALEDITVIQL
                     SLKGRKEKLLTDATFLLKLSQELAQAFHDQNIKASTNLGYTVKELLASHILAIEEQGC
                     FHLELSSLADSFGISYRHLLRVIHDMVKEGLIQKEKPKYFIKNRFALESLNIQP"
     gene            2879..4042
                     /locus_tag="VBD27_001215"
     CDS             2879..4042
                     /locus_tag="VBD27_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7025227.1"
                     /translation="MKIFMEKLSLLSLSLILLSTFSTSPALPQMISYYRDKGLPSPQV
                     ELLFSIPSMAIIFILLITPWLSKKLSEKHMIIFGLLLTALGGGLPVVSQNYLLVFVSR
                     LLLGSGIGFINARAISVISEYYQGKERRKLLGLRGSFEVLGNAGLTALVGLLLTFGWS
                     KSFMIYFLALPILVLYLVFAPKKVVKDTNDKIKTKGQKIPKADLTYIVALAILAGFVI
                     TINTGINLRIPLLVVEFGLGTPAQASLVLSAMMLMGIIAGMSFGQLIAMFHKQLIPIC
                     LVLFSLTLLGVGLPSNLMVLTISAMASGFLYSLMVTAVFSLVADRVEYSLVGSATTLV
                     LVFCNIGGASAAILLSCFDHLLGQINAVFYVYAILSLAVGMIYFLKRLFFKKM"
     gene            complement(4088..5071)
                     /locus_tag="VBD27_001216"
     CDS             complement(4088..5071)
                     /locus_tag="VBD27_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7025228.1"
                     /translation="MTEKYYNWATLGTGVIANELAQALEARGQKLYSVANRTYDKGLE
                     FANKYGIQKVYDHIDQVFEDPEVDIIYISTPHNTHISFLRKALANGKHVLCEKSITLN
                     STELKEAIDLAETNHVVLAEAMTIFHMPIYRQLKTLVDSGKLGPLKMIQMNFGSYKEY
                     DMTNRFFSRDLAGGALLDIGVYALSCIRWFMSEAPHNITSQVTFAPTGVDEQVGILLT
                     NPANEMATVSLSLHAKQPKRATIAYDKGYIELFEYPRGQKAVITYTEDGHQDIIEAGK
                     TENALQYEVADMEEAISGKTNHMYLNYTKDVMDIMTQLRQEWGFTYPEEEK"
     gene            5219..7114
                     /locus_tag="VBD27_001217"
     CDS             5219..7114
                     /locus_tag="VBD27_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEO7025229.1"
                     /translation="MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALML
                     STTDKWLADENKPSDTILNHFIAFHKMTADYQKREEVGVSPVLPLIEEYKGLQSFSEF
                     ASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNEKGKELL
                     ALWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWE
                     DFTKLAPELPLDAIFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSA
                     ANAYNAYLTDDIRIKSGVYSRALSGTPQAMDKKKAAYYLASGPYNQALGLWYAGEKFS
                     PEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIVKLNVITPHIGYPEKLPETYT
                     KKIIDPKLSLVENATNLDKISIAYGWSKWNKPVDRSEWHMPAHMVNAYYDPQQNQIVF
                     PAAILQAPFYALEQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFK
                     AFKKLTDKVVEQFDGLESYGAKVNGKLTVSENVADLGGVACALEAAQRESDFSARDFF
                     INFATIWRMKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKDGDNMWRPV
                     EKRVIIW"
     gene            complement(7164..8117)
                     /locus_tag="VBD27_001218"
     CDS             complement(7164..8117)
                     /locus_tag="VBD27_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEO7025230.1"
                     /translation="MKKLTKGSHIRVLSPSSSIERLGGFEANLLAKEVLEKLGFQVSF
                     SKHYLENDILYSASIASRVEDLHEAFADPSVDAILATIGGFNSNELLPYLDYDLISKN
                     PKIICGYSDSTAFLNAIFAKAKIQTYMGPAYSSFKMKEGQPYQTQAWLTAMTENHYEL
                     WPSEEWSSDPWYDPSKPRQFFPTEWKIYNHGKASGTIIGGNLSTFGLLRGTPYAPKIE
                     RYVLLIEEAEESNFYEFDRNLAAILQAYPHPQAILMGRFPKECGMTPQVFEYILSKHA
                     IFKEIPVIYDMDFAHTQPLLTVTIGAEISVDTTTLSLSIKE"
     gene            8284..8520
                     /locus_tag="VBD27_001219"
     CDS             8284..8520
                     /locus_tag="VBD27_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025231.1"
                     /translation="MSQLSLEERLNRLKDKYLSSKNQEFSNVHLSKVKKKKKKLIILE
                     KERCRCRLEREDTKKIDDKIEKLKKELIDVMKEE"
     gene            8522..>10482
                     /locus_tag="VBD27_001220"
     CDS             8522..>10482
                     /locus_tag="VBD27_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEO7025232.1"
                     /translation="MEETILIVSFLLFLILSNVINRIFPKLPLPFIQLVFGILSGLVF
                     HKSQVHIDPELFLAFVIAPLNFREGQESDIGSFVKYRTIILYLILPTVFLTAIVVGYV
                     AGHLLPVSLPLAACFALGAALGPTDAVAFISIAKRFQFPKRAENILKLEGLLNDASGL
                     VSFQFALTALVTGYFSLAKASLKLALAIMGGFLIGLLFAFLMRLCLTVLEKFDAADVT
                     GALLLELTLPFVAYFVADLLGFSAIIAVVVAGVMQANRLKKVTLFDAQVDRVTSVIWE
                     TLNFILNGLVFLIFGRELIRIIGPLLTSNAYSNFDLISIVVLVTCTLFLVRFLAVSRF
                     YAWRSFKYHKSFKKYWREIQLLTFSGVKGSVSIATILLLPKHSVIGELGYSLILFTVG
                     AVTLMSFLTGLLVLPKLAPPLQVKDDYLIRLSILTKVLSVLEEDGKSSENQASFYAVI
                     DNYNSRIRHLILEQESSDIKKDLAELQLMMLSIESDGLEAAYRYGNISIKEYRIYQRY
                     LKYLERQVNRSFVSNITYSLTILMRVIRGIVHEFLTFGQGIRNRKKHFKNRKRLLAEV
                     NRQHLTDLYLSNNDLILETLEDMEGYYNATLIDFLKNERIRDAELIKSGLFVERVIAR
                     ITPDILNETLRGYYLERRTISEFERDGLI"
BASE COUNT         3288 a         1647 c         2056 g         3491 t
ORIGIN      
        1 aattagttaa gaaaataaaa tttgttgatg aggtgatgga ttataccttg atagttgatc
       61 ggaactgtca ggtgaaatgt gttcctttag tgcaaacgaa cagtcataag gaactaagta
      121 atagcattgc atttgatatt aggtctcaag actttcatta tttaattgga gtgcatatgg
      181 atgatattat ctttggggat aaactctatt tgatgcaagg gataaaatgc aaaggaaaag
      241 tcattgtata tgataaaaat aatggcaaaa tgagtcgttt aaaaaattaa gagggctatt
      301 attatgctat actagtacta tattaaccat aaaaaaataa aatcagtaaa attagaaagg
      361 aggtttgtta tgtctaaaaa aatgaccatt aatgacatcg ctcaattatc aaaaacttcc
      421 aaaacaacgg tttctttttt cttgaatcaa aaatttgaaa aaatgtcgga tgagacgagg
      481 cagcgtattc aagaggtgat tgatgaaaca ggttatagac caagtacgat agcaagaagt
      541 ctaaattcta aaaaaacaaa actactaggt gtcttaattg gagatataac aaacacattt
      601 tcaaatcaaa ttgtaaaagg aattgagcac atcaccaaac aaaaaggcta tcaaattatt
      661 gtgggtaata gtaattatga tgccaaaagt gaagaagatt atattgaaaa tatgttaaat
      721 ttaggtgttg atggtttcat tattcagcct acatcaaatt ttcgaaagta ttctcgtatt
      781 ttaaaagaaa aaaagaaacc aatggtattt tttgatagtc aactttatga acataagacg
      841 agttgggtta aggctaataa ctatgatgct gtttatgata tgacacagga gtgtctaaat
      901 cgagggtata aaaagtttat tatgattact gctgatacga gcttgcttag tacacgtatt
      961 gagagagctt cgggattcat ggatgcttta aaggacaatg gtttcggtta tgacactttg
     1021 gtgattgaag atgatgatca ttcaaaaagt gatatagaag actttctaaa agcggtagtt
     1081 ccagataagg aggagacatt agtatttgca ccaaattgtt gggctttacc aatggttttt
     1141 actgccatga aaaatctcaa ttttgatatg ccacgagttg gtttagttgg gtttgacaat
     1201 attgaatgga cagacttttc atctcctaaa gtgtcaacaa ttgtccagcc ggcttatgaa
     1261 gaaggtgaac aagtagcaca gattttgatt aatcgtattg aaggagatga ctccgtcgat
     1321 aaccaacaga ttgtagattg ccagatgttt tggaaagagt cgacttttta aaaaaagatt
     1381 tcttatttta tttgctatta aaaaataaaa cctagagaaa attatctcta ggttatttgt
     1441 ccttattcta aaataagtaa gccttctttg acagcgaaag gatttttctc attgatacgg
     1501 tcgtaaaaca taataccatc aatgtggtca atttcatgtt gaacaacaat agagttgtaa
     1561 ccttttaatt tgagtcgatg tttttcacca gttttgtcga aatattcaat agttacacgc
     1621 gcgtggcgga cgacatagcc aggtacttca cgatctactg ataggcatcc ctccccgtca
     1681 gataaagcag catcttgaac agaatgcgat acaactttag ggttgtacat tacttcttgg
     1741 aggctataag cttctttagg tggatttcct tgagcatcct caacgtttgg aacaaggacg
     1801 gcaatgatgc gttttgagat gtctaattgt ggtgcggcta aaccaacacc accacgtaag
     1861 cctagcttct cagccataat tggatcttga gagtgtttta aaaattgcat cattttttct
     1921 ccgagaattt cttctttctc agaaagtggg aatgtgactt cttcagccac tttgcgaagg
     1981 gttggattgc cttcgcggat aatgtcattc atgtcaatta aatgactagc ttttactaat
     2041 ttatcaatag cagacatatt ttctcctaac tgtattctct gatttagttt agcataaaat
     2101 aattaaatga taaagctacg cttcatggct gaatatttaa tgattctagt gcgaaacgat
     2161 ttttgataaa gtattttggt ttttcttttt ggataaggcc ttctttgacc atatcatgga
     2221 taactcttaa taaatgacga taactgatgc caaacgaatc agctaaacta cttagttcaa
     2281 ggtgaaagca accttgttct tcaattgcta aaatatgact tgcaagtaac tctttgacgg
     2341 tataccctaa attagtagac gctttgatgt tttgatcatg aaaagcctga gctagctctt
     2401 gactaagttt taggaggaaa gttgcgtctg ttaacagttt ctctttccgt cctttaagtg
     2461 ataattgaat gactgtaata tcctctaagg caataacaga tgatacaatt tgtctattag
     2521 tcaaaagttc aatatcacca atcagtgttg gttttgtttt aatatctaaa atatgttctt
     2581 tgccgttaaa gagtcgacgt acaattttaa aacgtccctt gacgatgtag tgcaagtatt
     2641 ctaattgttc tccctgttta cagacggatt ggccggcagt tagggtgatt acttgtaaat
     2701 ccttgaaata atgttttaga aagatagtct ccaaacagta gtcttggcag tagttttcta
     2761 tatccatgta ttgactccct atttgagata gttttaggac atatgtcata tatttctagt
     2821 ttaatattga ttatacttta tttatctaaa aaaagacatc agaaagaggt attgtcactt
     2881 gaaaatcttt atggagaagc taagtttact ctctttatca cttatcttgc tatcaacttt
     2941 ttcgacatca ccagccctac cacaaatgat atcttattat agagataagg gcttaccatc
     3001 accccaagtt gaattattat tttcaattcc ttcgatggct attatcttca tcttgcttat
     3061 cacaccttgg ttaagtaaaa aactttctga gaaacatatg attatctttg gactattgct
     3121 aacagcatta gggggaggat tgccggttgt cagtcagaac taccttttag tatttgtatc
     3181 gcgtctttta ttggggagtg gtatcggttt tattaatgct agggccatta gtgtgattag
     3241 tgaatattat caaggcaaag aaagaagaaa actcttagga ttaagggggt cctttgaagt
     3301 tttaggaaat gctggtttga cagccttagt tgggctattg ttgacttttg gttggtctaa
     3361 atcatttatg atttattttt tagctttacc aatcttagtt ctatatcttg tttttgcgcc
     3421 taagaaagtg gtgaaagata caaatgacaa gattaaaact aaaggtcaaa aaatccctaa
     3481 agctgatttg acttatattg tcgccttagc tattttagca ggatttgtca taactataaa
     3541 tactgggatt aatcttagga tacctctgct agtagtagaa tttggtttgg gtacgccagc
     3601 tcaagctagt cttgttctta gtgcgatgat gcttatggga ataatagcag gtatgagttt
     3661 tggtcagttg atagctatgt ttcataaaca attaattcca atctgcctcg tcttattttc
     3721 tttaacattg cttggagttg gattgccttc taatctaatg gtactaacaa ttagtgcgat
     3781 ggcgtcaggt ttcctttaca gcctgatggt cactgctgtg tttagcctag tagcagaccg
     3841 tgttgaatat tcgttggttg gttctgcaac gacattagtt ttagtatttt gtaatattgg
     3901 tggagctagt gcggctattc ttctgtcttg ttttgatcat ttactaggac aaataaatgc
     3961 agttttctat gtttatgcta ttttaagttt agcagttggt atgatttatt tccttaaacg
     4021 cctatttttt aagaaaatgt agtgtatatt aactaaaaag ggcttgacaa tcattgtcaa
     4081 gctctcttca tttttcttct tctgggtagg taaatcccca ttcttgacgt agctgtgtca
     4141 tgatatccat aacatctttg gtatagttta agtacatgtg gttagttttt cctgaaatgg
     4201 cttcttccat atcagctacc tcatattgga gagcattttc agttttgcca gcttcgataa
     4261 tatcttgatg cccatcctca gtataagtaa taactgcctt ttgtcctcgc ggatattcaa
     4321 aaagttcaat gtagccttta tcgtaagcga tagttgctcg tttaggttgt tttgcatgta
     4381 aactaaggct gacagtcgcc atctcatttg ctgggttggt tagtaggata ccaacttgtt
     4441 catcaacccc tgttggtgca aatgtaactt gagaggtaat gttgtgaggt gcttctgaca
     4501 taaaccagcg aatacaagaa agtgcataaa caccaatgtc cagcaaagca ccgcctgcta
     4561 ggtcacgact gaaaaaacgg ttagtcatat catattcttt ataacttccg aaattcattt
     4621 gaatcatttt taacggtcct aattttccac tatcaactaa tgtttttaat tggcggtaaa
     4681 ttggcatatg aaaaatagtc atggcttcag ctaagacaac atggttagtt tcggctaaat
     4741 ctatggcttc tttaagctca gtactattta aagtaataga tttttcgcaa agaacgtgct
     4801 taccatttgc taaagccttt cgtaaaaatg agatgtgagt attgtgggga gtagagatat
     4861 aaatgatatc cacttcaggg tcttcaaata cttgatctat gtgatcataa actttttgga
     4921 taccatattt gttagcaaat tcaagtcctt tgtcgtaagt tctattagct acagaatata
     4981 atttttgtcc acgtgcttcc agtgcttggg ctaattcgtt ggcaataacg ccggttccaa
     5041 gcgttgccca attataatat ttttcagtca taacaacctc cttgttggtt tatagtatag
     5101 caaaaataac aaaatgatat ttaaagaaag caccaatatg agagtatttt taatagtcaa
     5161 actctgaatt ttctgttaga ctgtaagaga ttacatatga gatattaagg agaaaataat
     5221 gataaaatat caagatgatt tttaccaagc ggttaatggg gaatgggcta agacagctgt
     5281 tattcctgat gacaaaccaa ggacaggtgg tttttctgat cttgctgatg acattgaagc
     5341 attaatgctg tcaacgacag ataagtggct ggctgatgaa aataaaccaa gtgatacgat
     5401 tttaaatcat tttattgctt ttcataaaat gacagctgat tatcaaaaac gtgaagaggt
     5461 aggagtatca ccagtattac cattaattga agaatataaa ggtctacaat ctttctctga
     5521 gtttgcatca aaagttgcag aatatgaact agaaggtaaa ccaaatgaat ttccatttgg
     5581 tgtggctcct gactttatga atgctcaatt gaatgtactt tgggctgaag cgcctgggat
     5641 tattttaccg gatacaactt attatagtga agataatgaa aaaggaaagg aactccttgc
     5701 tctttggcgt aaaagtcaag aagacttact gcctttgttt ggcctttcag aacaggaaat
     5761 taaagatatc ttggataagg ttttagcttt ggatgcaaaa ttggcacaat atgtcttatc
     5821 tcgcgaagag agttcggaat atgttaaact ttaccatcct tataactggg aagatttcac
     5881 aaaattagca cctgaacttc cattagatgc tattttccaa aagatattag gacaaaaacc
     5941 agataaagtt attgttcctg aagaacgttt ctggacagaa tttgcatctg actattactc
     6001 agaatctaat tgggaattat taaaggcaga tcttatttta tcagctgcga atgcctataa
     6061 tgcttactta actgatgata ttcgtatcaa atcgggagtt tacagtcgag ctttatcagg
     6121 cacaccacaa gcaatggata agaaaaaagc agcttactac cttgcttcag gaccttacaa
     6181 tcaagctcta ggtttgtggt atgcagggga aaaattctct ccagaagcta aagctgatgt
     6241 tgagcataag attgcaacga tgattgatgt ctataagtca cgcttagaaa aggcggattg
     6301 gcttgcacag tcaacacggg aaaaagctat tgtgaaactt aatgttatca cgccacacat
     6361 tggataccct gagaaactac cagagactta tactaaaaaa atcattgatc ctaaactatc
     6421 gcttgtcgaa aatgctacta atttagacaa aatttcaata gcctatggtt ggagtaaatg
     6481 gaataaacca gttgatagaa gtgaatggca tatgcctgcc cacatggtca atgcgtatta
     6541 tgatccacaa caaaaccaaa ttgtcttccc agcagccatt ttacaagcac cattttatgc
     6601 tttagagcaa agttcatcag caaattatgg gggaattggt gcagtgattg cacatgaaat
     6661 atcacacgct tttgacacta atggagcttc atttgatgaa catggaagtt tgaataattg
     6721 gtggactgat gaagatttta aagctttcaa aaaacttaca gataaagtag ttgagcagtt
     6781 tgatggactg gaatcttatg gcgcaaaagt caatggtaaa ctaacagtat cagaaaatgt
     6841 tgcagattta ggtggagttg cttgtgcttt agaagctgcg cagcgtgagt cagatttttc
     6901 agcgagggat ttctttatta actttgcgac aatttggcgt atgaaggcac gtgatgagta
     6961 tatgcaaatg ttagctagtg tagatgttca cgccccagca caatggcgaa ctaatattac
     7021 agtaacgaat tttgaagaat tccataaaga gtttgatgtt aaagatggtg ataacatgtg
     7081 gcgtccagtt gaaaaacgtg ttattatttg gtaactctaa atgataaact cccgttactc
     7141 agatgagtag caggagtatt tttttattct tttatagata aagataatgt agtagtgtca
     7201 acgcttatct cagctccaat agtcactgtc aaaagaggtt gggtgtgcgc aaaatccata
     7261 tcataaataa caggaatttc cttgaaaata gcatgttttg aaaggatata ttcaaagact
     7321 tgtggagtca tgccgcattc tttaggaaag cgccccataa gaatagcttg tggatgagga
     7381 taagcttgta aaattgcagc aaggttgcgg tcaaattcat aaaaattact ttcctcagcc
     7441 tcttcaatca agaggacata gcgttcaatt ttcggtgcat aaggggtacc tcgaagtagg
     7501 ccaaacgtag agagattacc tccgataatc gtaccggaag cttttccgtg attataaatt
     7561 ttccattctg ttgggaaaaa ttggcgtggt ttgctgggat cataccaggg atcactagac
     7621 cattcttcac taggccaaag ttcatagtgg ttttctgtca tggcagtaag ccaagcttgt
     7681 gtttggtaag gttgaccttc cttcattttg aaagaagaat aagcaggacc catgtaagtt
     7741 tggatttttg ctttggcaaa aatagcatta aggaaggcgg ttgaatctga gtaaccacaa
     7801 attattttgg gattcttaga gattaaatca taatctaaat atggtaaaag ctcgttggaa
     7861 ttgaatccgc caatggttgc taatatggca tcaacagaag ggtcggcaaa tgcttcatgt
     7921 aaatcttcta cgcggctagc aatcgaagca gaatagagga tatcattttc tagatagtgc
     7981 ttagagaatg acacttgaaa tcccagcttt tctagtactt ctttggctaa taggttagcc
     8041 tcaaaaccac ccagacgttc aatggaagaa gagggactaa gaacacgaat gtggctgcct
     8101 tttgttaatt ttttcattgg ttaacctcct atttaaattg ttgttagtct accacatatg
     8161 gagataatga gcaacgatat tcttaaattc tatcaattta agaaatgcgt gttatactag
     8221 agatagccga tgaaagaaaa aatacttatt attaataatt tggttattaa ataggaggtc
     8281 atgatgtctc aattatcttt ggaagaacga ttgaatcgac taaaagataa atacctttct
     8341 tctaaaaatc aagaattttc aaatgttcac ttaagtaaag taaagaagaa aaagaagaaa
     8401 ttaataatac ttgaaaaaga aagatgtcgc tgtcgattgg agagagaaga tactaaaaaa
     8461 atcgatgata aaattgagaa attgaagaaa gaattgatag atgttatgaa ggaggaataa
     8521 tatggaagaa actattctaa tagtcagctt tctcctgttt ttgattttat ctaatgttat
     8581 caataggata tttcctaaat taccattacc atttatccag ttggtctttg ggattctctc
     8641 tggcttagtt tttcataagt cacaagtcca tatcgatcct gagttatttc ttgcctttgt
     8701 tattgctcct ttgaacttta gggaaggtca agaaagtgat attggtagtt ttgtaaagta
     8761 tcggactatt attctttact taatcttacc aacggtattt ttaacagcta ttgtcgttgg
     8821 ttatgtagca gggcaccttt tgccagttag tttaccactt gcggcatgct ttgcgcttgg
     8881 agcagcctta ggcccaacag atgccgttgc ctttatatcg attgctaaga ggtttcaatt
     8941 tcctaaacga gcagagaata tcctcaaatt ggaaggtctt ttaaatgacg ctagtggtct
     9001 agtttccttc cagtttgctt taacagccct tgtaacaggt tatttctcct tagcaaaagc
     9061 tagtctcaag ttagcattgg ccataatggg tggtttttta attggcttgc tttttgcttt
     9121 tctaatgagg ttatgtctca ctgtcctaga gaaatttgat gctgctgatg ttacaggggc
     9181 tctacttttg gaattaacct taccttttgt tgcttatttc gttgctgatt tgttgggatt
     9241 ttcagctatt attgcggtgg tagtggcagg agtaatgcaa gcaaatcgtt tgaaaaaagt
     9301 gactctcttt gatgctcaag tagatcgcgt tacctctgtt atttgggaaa ccttaaattt
     9361 tattttaaat ggtttagttt tcttaatatt tggaagagaa ctgatacgta tcattggtcc
     9421 tcttttaaca agtaacgctt atagtaattt tgatcttatt agtattgttg ttttagttac
     9481 ttgcacactt tttctagttc gctttctagc agtttctcgc ttttatgctt ggagatcttt
     9541 caaataccat aaatcattta aaaagtattg gagagagatt caactgttga ctttttcagg
     9601 agttaaagga agtgtttcca tagcaacaat actcttatta cccaagcact cagtcatagg
     9661 agagttgggg tatagtttga ttcttttcac tgttggtgca gtaacgttaa tgagtttttt
     9721 aacgggactt ttagtattac caaagttagc gcctccctta caggtgaaag atgattattt
     9781 aatacgtcta tcgattttaa caaaagtatt atctgtctta gaagaagacg gtaagagttc
     9841 tgaaaatcaa gcttcatttt atgcagttat agataattat aatagccgta ttcgtcatct
     9901 cattttagaa caagaatcta gtgacattaa gaaggactta gctgaattgc aactgatgat
     9961 gcttagtata gaaagcgacg ggcttgaagc tgcttatcgt tatggcaata tttctatcaa
    10021 agaatatcgt atttaccagc gttatttaaa atacttagaa cgtcaagtta atcgtagctt
    10081 tgtgtcaaat atcacttata gtttaacgat tcttatgcgt gttattcgtg gtattgttca
    10141 tgagttttta acatttggac aaggaatacg aaatcggaaa aaacatttca aaaacaggaa
    10201 acggttgtta gcagaagtca atcgtcagca cttaacagat ttgtacttat caaataatga
    10261 cctcatttta gaaacgctag aagatatgga aggttattat aatgcaactt tgatagattt
    10321 cttaaaaaat gaacgcattc gagacgcaga attaattaaa tcaggtttat ttgtagaacg
    10381 tgtcattgca cgcataacgc cagatatttt aaatgaaacc ctaagaggat attacttgga
    10441 acgcagaaca atctctgaat ttgaaaggga cggtttaatt ag
//
LOCUS       DATRQA010000064        10403 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.064, whole genome shotgun sequence.
ACCESSION   DATRQA010000064 DATRQA010000000
VERSION     DATRQA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.064"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            100..2601
                     /locus_tag="VBD27_001221"
     CDS             100..2601
                     /locus_tag="VBD27_001221"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO7025233.1"
                     /translation="MTFYNHKEIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDESKLVENEIEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVVK"
     gene            complement(2691..3299)
                     /locus_tag="VBD27_001222"
     CDS             complement(2691..3299)
                     /locus_tag="VBD27_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO7025234.1"
                     /translation="MLHSKKIHSLSLIAVLSLATYTSLQPNHVAAEQSQKTSTVHMSQ
                     KTIEHKLKVADKEAAPLYAKIDHIQRHIEVKKAKDLKVIELYINKDINQLEKQNKRLL
                     TKFYTSIDNQTWDSTSEVKKLIDKTTLSTNEKDRLKLYFEQRAYLETRLNDRYQKFDN
                     SIENQNKELKILTSKIEKIYQKHGITKEVLKTYYAKKTVRAD"
     gene            complement(3392..4780)
                     /locus_tag="VBD27_001223"
     CDS             complement(3392..4780)
                     /locus_tag="VBD27_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7025235.1"
                     /translation="MKKKSLISNLILINSITLFLGFYLIYFSVNYVIKDYVHQMTRNS
                     MQANFTILDSIYNNKKIPDENMVNQDSIFVWSYYAIFDQHYRMKYGTDNHKKESHDII
                     RYLTQKRLWQISKEKDGMFITIKKKTYYVMTKDYSGILVDGSIKKAPKAQSQLFHVIN
                     FSDITYTQHLITKINHFLIVILVLTYIPMLFIMRKTFTGIRESIQSVQTYISSLWKNQ
                     GNHQSSQKEIVFSDFDPLLLESQEMANRIYQAEESQRNFFQNASHELRTPLMSIQGYT
                     EGVQEGIIDAELAHSVILQESKKMKQLVDDIILLSKLDSNLSDQKDKFSLNELLNSII
                     AYFKPLANKQKISITYRPDKHEKLLKGNEELIQRAINNILSNALRYAVSHIEISYTNQ
                     KLTISNDGPAISKEDLPYIFDRFYKGHGGQTGIGLAMTKEIIKQHHGNIIAESDSTST
                     TFTIYFNCHKIA"
     gene            complement(4777..5462)
                     /locus_tag="VBD27_001224"
                     /pseudo
     CDS             complement(4777..5462)
                     /locus_tag="VBD27_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(5695..>10403)
                     /locus_tag="VBD27_001225"
     CDS             complement(5695..>10403)
                     /locus_tag="VBD27_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO7025236.1"
                     /translation="KQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSDIAV
                     EQQVKDSPTSIANETPTNNTSSALASTAQDNLVTKANNNSPTETQPVTEPHSQATETF
                     SPAANQPVESTQEVSKTPLTKQNLAVKPTPAISKETPQNIDSNKIITVPKVWNTGYKG
                     EGTVVAIIDSGLDTNHDALQLNDSTKAKYQNEQQMNAAKAKAGINYGKWYNNKVIFGH
                     NYVDVNTELKEVKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSDEKR
                     GTGPALYVKAIEDAVKLGADSINLSLGGANGSLVNADDRLIKALEMARLAGVSVVIAA
                     GNDGTFGSGASKPSALYPDYGLVGSPSTAREAISVASYNNTTLVNKVFNIIGLENNKN
                     LNNGLAAYADPKVSDKTFEVGKQYDYVFVGKGNDNDYKDKTLNGKIALIERGDITFTK
                     KVVNAINHGAVGAIIFNNKAGEANLTMSLDPEASAIPAIFTQKEFGDVLAKNNYKIVF
                     NNIKNKQANPNAGVLSDFSSWGLTADGQLKPDLSAPGGSIYAAINDNEYDMMSGTSMA
                     SPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTGAYTSPRQQGA
                     GIIDVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTYLNT
                     DQVKDGFVTLAPQQLGTFTGKTIRIEPGQTKTITIDIDVSKYHDMLKKVMPNGYFLEG
                     YVRFTDPVDGGEVLSIPYVGFKGEFQNLEVLEKSIYKLVANKEKGFYFQPKQTNEVPG
                     SEDYTALMTTSSEPIYSTDGTSPIQLKALGSYKSIDGKWILQLEQKGQPHLAISPNDD
                     QNQDAVALKGVFLRNFNNLRAKVYRADDVNLQKPLWVSAPQAGDKNYYSGNTENPKST
                     FLYDTEWKGTTTDGIPLEDGKYKYVLTYYSDVPGSKPQQMVFDITLDRQAPTLTTATY
                     DKDRRIFKARPAVEHGESGIFREQVFYLKKDKDGHYNSVLRQKGEDGILVEDNKVFIK
                     QEKDGSFILPKEVNDFSHVYYTVEDYAGNLVSAKLEDLINIGNKNGLVNVKVFSPELN
                     SNVDIDFSYSVKDDKGNVIKKQHHGKDLNLLKLPFGTYTFDLFLYDEERANLISPQSV
                     TVTISEKDSLKDVLFKVNLLKKAALLVEFDKLLPKGATVQLVTKTNTVVDLPKATYSP
                     TDYGKNIPVGDYRLNVTLPSGYSTLENLDDLLVSVKEGQVNLTKLTLINKAPLINALA
                     EQTDIISQPVFYNAGTHLKNNYLANLEKAQTLIKNRVEQTSIDNAIAALRESRQALNG
                     KETDTSLLAKAILAETEIKGNYQFVNASPLSQSTYINQVQLAKNLLQKPNVTQSEVDK
                     ALENLDIAKNQLNGHETDYSGLHHMIIKANVLKQTSSKYQNASQFAKENYNNLIKKAE
                     LLLSNRQATQAQVEELLNQIKATEQELDGRDRVSSAENYSQSLNDNDSLNTTPINPPN
                     QPQALIFKKGMTKESEVAQKRVLRVTSQTDNQKIKTNKLPKTGESTPKITYTILLFSL
                     SMLGLATIKLKSIKRE"
BASE COUNT         2978 a         1865 c         2015 g         3545 t
ORIGIN      
        1 tggggagaga acctgctgtt gagggtattt tcaccttttt tattgctgtt tttcgtgtta
       61 taataagagg actatacttt tttgacatgg agaagtgcga tgacattcta taatcacaaa
      121 gaaatcgaac ccaaatggca agctttttgg gcagataatc acacctttaa aacaggaaca
      181 gacgcatcaa aacctaagtt ttatgcccta gatatgttcc catacccatc tggagctggt
      241 ctccacgttg gtcacccaga aggctataca gcaacggata ttctcagccg tttcaaacgt
      301 gcccaaggtc acaatgtcct ccacccaatg ggttgggatg cttttggact tcctgctgag
      361 cagtacgcta tggatactgg taatgaccca gctgagttta cagctgaaaa catcgctaac
      421 ttcaaacgcc agattaatgc tcttggtttc tcatacgact gggatcgtga agttaacaca
      481 actgacccta actactacaa gtggacacag tggatcttta ccaagcttta tgaaaaaggt
      541 ctagcttacg aagctgaagt tccagttaac tgggttgaag aattgggaac agctatcgcc
      601 aacgaagagg ttcttccaga tggtacatca gaacgtggtg gctacccagt tgtcagaaaa
      661 ccaatgcgcc aatggatgct caaaatcact gcctatgcag agcgcttgct agaagacttg
      721 gaagaagttg actggccaga atcaatcaaa gacatgcaac gcaactggat tggtaaatca
      781 actggtgcta atgtaacctt caaggttaag gatacagata aggacttcac tgtctttaca
      841 acacgtcctg acaccctttt tggtgcaact tacgcagttc ttgctcctga gcatgccttg
      901 gttgatgcta ttacaacagc tgaccaagca gaagccgttg ccgaatacaa acgtcaagca
      961 agtctcaaat cagaccttgc tcgtacagac cttgccaaag aaaaaacagg tgtctggaca
     1021 ggtgcctatg ccattaaccc agttaatggt aaagaaattc cagtttggat tgcggactat
     1081 gtccttgcca gctacggtac aggtgctatc atggctgttc cagcccacga tgaacgtgac
     1141 tgggaattcg caaaacaatt taagctagac atcattccag ttcttgaagg tggtaatgtt
     1201 gaagaggctg cctttacaga agacggtctt cacatcaact cagacttcct agatggtctc
     1261 gataaagcgt cagctattgc caaaatggtc gaatggcttg aagcagaagg tgttggtaat
     1321 gagaaagtca cttaccgtct ccgcgactgg ctcttcagcc gtcaacgtta ctggggtgag
     1381 ccaatcccaa tcatccactg ggaagatggt acatcaacag cggttcctga gagtgaattg
     1441 ccattggttc ttccagtaac taaggatatc cgtccttcag gtactggtga gagcccactt
     1501 gctaacttga cagactggtt ggaagtgact cgtgaagatg gtgttaaagg tcgtcgtgag
     1561 actaacacca tgccacaatg ggcaggttca agctggtact atctccgtta catcgatcca
     1621 cacaatactg aaaaattggc tgacgaggaa ctcctcaaac aatggttgcc agttgatatc
     1681 tatgtcggtg gtgcagagca cgccgtgctt caccttctct acgctcgctt ctggcacaag
     1741 gttctttacg atctcggtgt cgtacctacc aaagagccat tccaaaaact ctttaaccaa
     1801 gggatgattt tgggaacaag ctaccgcgat agccgtggtg ctcttgtggc aactgataag
     1861 gttgaaaaac gtgatggtag cttcttccat gtggaaactg gggaagagct tgaacaagca
     1921 ccagctaaga tgtctaaatc acttaagaac gttgtcaacc cagatgatgt tgttgaacag
     1981 tacggtgctg acacccttcg tgtttacgag atgttcatgg gaccacttga cgcttcaatc
     2041 gcttggtctg aagaaggtct tgaaggctca cgtaagttct tggatcgtgt ttaccgtttg
     2101 atcacgacta aagagattac tgaagaaaat agcggagccc ttgacaaggt ttacaacgaa
     2161 accgttaagg cagttactga gcaagttgac caaatgaaat tcaacacagc tatcgctcaa
     2221 ctcatggtct ttgtcaatgc agccaacaag gaagacaaac tctttagcga ctatgccaaa
     2281 ggctttgttc aattgattgc accattcgca ccacacttgg gtgaggaact ctggcaagtc
     2341 ttgacagcct caggtgagtc aatctcttac gtaccatggc caagttatga cgaaagcaaa
     2401 ttggttgaaa acgaaattga aatcgttgtc caaatcaaag gtaaggtcaa agctaaactt
     2461 gtagttgcta aagacctttc acgcgaagaa ttacaagaag ttgcccttgc taacgaaaaa
     2521 gtccaagccg aaatcgcagg caaagacatc atcaaagtca tcgcagtacc aaacaaactg
     2581 gttaatattg tggtgaaata agataaagat tttactagaa atagtgatat ttacgcgtat
     2641 aaaatcccaa aagtttatga acttttggga tttttctatt tttagctaaa tcagtcagct
     2701 cgtactgttt ttttagcata gtaagttttt aatacctctt ttgtaatacc atgtttttga
     2761 tagatttttt ctatttttga cgttaatatt tttagttctt tattttggtt ttcaatagag
     2821 ttatcaaatt tttgatagcg gtcgttcaac cttgtctcaa ggtaagcacg ttgttcaaaa
     2881 tataatttta atctatcttt ttcgttagtg gatagggttg tcttatcaat caattttttg
     2941 acttcacttg tgctatccca tgtttgatta tcaatagaag tatagaattt agttagtaga
     3001 cgtttatttt gcttctctag ttggttgata tctttgttaa tatacaattc aataactttt
     3061 aaatcttttg cttttttgac ttcaatatgt cgttggatat ggtcgatttt agcgtagaga
     3121 ggagcagctt ctttatctgc aacttttaac ttatgttcaa tagttttttg actcatatga
     3181 acagttgatg ttttttgtga ttgttcagcc gctacatgat ttggttgtaa actcgtatat
     3241 gttgctaaag agagaacggc aataagcgat aaggaatgta tttttttaga atgtaacata
     3301 taaaaactca ctttctttta gaataacttt ttgatctata ggtattatag gttaaaaaag
     3361 caataacttc tttgaatttt tgtaggaaaa gttaggcaat tttgtggcaa ttgaaataaa
     3421 tcgtaaaagt tgttgaggtc gaatcactct cagcgatgat attaccatga tgttgcttga
     3481 taatttcttt agtcattgca agtccaatac ctgtttggcc gccatgtcct ttatagaagc
     3541 gatcgaaaat ataaggcaag tcttcttttg aaatggctgg tccgtcgttt gagatagtta
     3601 gtttttgatt tgtgtaagag atttcaatat gagaaacagc atagcgtagt gcgttactaa
     3661 ggatattatt gatagctcgt tgaatcaact cttcgttacc ttttagtaac ttttcatgct
     3721 tgtctggtct ataagtgata ctaatctttt gtttattagc taggggtttg aaataagcaa
     3781 taatactatt aagtaattca tttaaagaaa atttatcttt ttgatcactt aagttactat
     3841 ctaatttaga gagtaagata atatcgtcta cgagctgttt catttttttg ctctcttgca
     3901 gaataacaga gtgtgcgagc tcggcatcaa taataccctc ttgaactccc tcagtatatc
     3961 cttggatgga cattaaaggt gtccgtaact cgtgggaggc attttgaaag aagtttcttt
     4021 ggctctcttc tgcctggtag attcgatttg ccatttcttg gctctcaagt agaagaggat
     4081 cgaaatcaga aaagacaatt tctttttgac tagattgatg attgccttga tttttccaaa
     4141 ggctggaaat ataagtttgt actgattgaa tagattctct aattccagtg aaagtcttcc
     4201 tcataataaa tagcatggga atataggtta gtactaagat aactataaga aaatgattaa
     4261 ttttagtaat tagatgctgg gtgtaagtaa tgtctgaaaa gttaatcaca tgaaaaagtt
     4321 gcgactgtgc tttaggagct ttttttatgc taccatcaac aaggatacca gagtagtctt
     4381 tggtcattac ataataggtt tttttcttga tggttataaa cataccatct ttttccttgg
     4441 aaatttgcca caatcttttt tgggtaagat agcgaataat atcatggctc tcttttttat
     4501 gattatcagt gccatatttc atacgataat gctggtcaaa aatagcatag taagaccaaa
     4561 caaagatgct atcttggtta accatatttt catctggtat tttcttgttg ttatagatac
     4621 tatctaaaat ggtgaaattc gcctgcatgg aattccttgt catttgatgg acatagtctt
     4681 taatgacata gttgacagaa aaatagatga ggtaaaaacc aagaaataga gtgatgctat
     4741 taataaggat aagattgcta attaaacttt tctttttcat ttgtcctctt tgattaattt
     4801 gaagccgtaa ccccagacag ttttgatact aacctgactg tggtattgct taagtttttt
     4861 acgtaatcgt ttaatagtgt catcaactgc tctgctttca atatcatcaa atccccaaat
     4921 acgattaagc agtgtttccc tagaaaaagc agtttcaggt ttttcaatta atactaaaag
     4981 aaaatcaaat tctgtcttgg ttaagggcat ttcttcattt gataataggg ctattcgctt
     5041 ttcagtatcc agaattaaat ctagaactga tactgagtgt catttttgat tgctttttca
     5101 tcaaggcgtt tgaacaatgc tttaacatgt agactaagtt ttataggtga aaaaggcttg
     5161 gtgaagtaat cgtcagttcc taaattaaaa gccgtaatga aatcaacatc agaatctcta
     5221 gctgttaaca tgataatagg aatattatca tattggcgga tgcttttcat aacatctaaa
     5281 ccattagttc caggcatcat tatgtctaat ataactaaat cgggtttttg gtttttatag
     5341 gcttctaaga gaaggtctcc agtttcaaat gctcgaacag tgaaaccatc atgttctaaa
     5401 aaaggaacca ccaagtcacg aatattcttt tcatcatcag cgaaataaat tatttttgtc
     5461 atgtatacct caatacccat agttataatt tgacttttag ctagctgttt atgttatatc
     5521 aaatataaca aaaaaagagt ttttttccta ttctttgaag tgttatagtg ttataaacca
     5581 agatattgca gagaaaaggt ctttaggaac atcattttaa gctcctcaaa aaggcaccag
     5641 tcactaacta ttaatagcta gtaattgatg ccttgctcta ttttttaaag tattttattc
     5701 tcttttgata gactttagtt tgattgttgc cagacctagc atagagagac taaatagcaa
     5761 tattgtatag gttattttag gagtgctttc gcctgtttta ggaagcttgt ttgtctttat
     5821 cttttgatta tcggtttggc tagtcaccct taagacacgc ttctgagcaa cctcactttc
     5881 tttagtcatg ccttttttga atatcaacgc ctggggctga tttggcggat tgataggtgt
     5941 ggtattgaga gagtcattat cattgagtga ttgactataa ttctctgcgg aagaaactct
     6001 atcgcgacca tcaagttctt gttcggttgc ttttatttgg tttaataact cttcaacttg
     6061 agcttgtgta gcttgtctat tggaaagcag caattctgct ttcttgataa ggttattata
     6121 attttcttta gcaaattgac tggcgttctg atatttagat gatgtttgtt tcagaacatt
     6181 tgctttaatt atcatatggt gtaaaccaga gtaatcagtt tcatgaccat ttaattgatt
     6241 tttagcaata tcaagatttt ctaaggcttt gtctacttct gattgagtga cgttaggttt
     6301 ttgtagaaga tttttcgcca attggacttg attgatataa gttgattggc ttaatggact
     6361 agcattaaca aattgatagt ttcccttgat ttctgtttca gctaaaatag cttttgccag
     6421 taaagaagta tctgtttctt taccgttaag agcttggcga ctttctctca aagcagcaat
     6481 agcattatca atacttgttt gttccactct atttttaatt aaagtttgtg ccttttcaag
     6541 attagctagg taattatttt ttaagtgagt tccagcatta taaaacacag gttgggaaat
     6601 aatgtcagtt tgttctgcta gggcattaat tagaggagct ttattaatca gcgtcaattt
     6661 tgttagattt acttgacctt cttttacgga tacaagtaaa tcatctaagt tctctaaagt
     6721 gctataccca ctaggcagcg ttacgtttaa acgatagtct cctacaggta tgtttttacc
     6781 atagtcagta ggagaataag ttgcttttgg tagatcaaca acagtatttg tcttagtaac
     6841 caactggact gttgctcctt ttggtaaaag cttgtcaaat tcaacgagta aggctgcttt
     6901 cttgagtaag ttaactttaa ataagacgtc tttaaggcta tctttttcag aaatagttac
     6961 agtgacactt tggggactga ttagatttgc tcgttcctca tcgtataaga ataggtcaaa
     7021 cgtataggta ccaaaaggca atttcagtaa attgaggtct ttcccgtgat gttgcttttt
     7081 gatgacatta cctttgtcat ctttgacaga gtaagagaaa tcaatatcga cattactgtt
     7141 aagctcaggg ctaaacactt tgacgtttac taaaccattt ttattgccaa tattgatcaa
     7201 atcttcgagt tttgctgaca ctagattgcc tgcataatct tcaacagtgt agtagacatg
     7261 agagaaatca ttaacctctt taggtagaat aaagctacca tccttttctt gtttgataaa
     7321 tactttgtta tcttcaacaa ggataccgtc ttctcctttt tgacgtaaga cgctattata
     7381 atgaccatct ttatcttttt ttaagtaaaa aacttgttct ctaaagatac cagattcccc
     7441 gtgttctact gcaggacgag ctttgaagat acgtctatct ttgtcataag ttgctgttgt
     7501 tagtgtagga gcttgtctat ccaaagtgat atcaaatacc atttgttgtg gcttagagcc
     7561 agggacatca gagtagtatg ttaaaacgta tttgtatttt ccatcttcta aaggaatacc
     7621 atcagtagtg gttcctttcc attctgtgtc atataaaaat gtagattttg gattttcagt
     7681 atttccgctg tagtaatttt tatctcctgc ttggggagca cttacccata gtggtttttg
     7741 taaattaaca tcatctgcac gatagacttt ggctcttaaa ttattgaaat tacgtaagaa
     7801 aacacctttc agtgcaacgg catcttgatt ttggtcatca ttaggtgaaa tggctagatg
     7861 aggctggcct ttttgctcta gttgtaagat ccattttcca tctatagact tatagcttcc
     7921 caaggctttc aattggatag gactagtacc gtctgttgag tagataggct ctgaacttgt
     7981 agtcattaag gcagtataat cttctgaacc aggaacttcg tttgtttgtt tcggttggaa
     8041 ataaaatccc ttttctttgt tagcaacaag cttataaatg gatttttcta aaacttctaa
     8101 gttttggaat tctcccttaa atccaacata aggaatacta agaacttccc caccatcaac
     8161 agggtctgta aaacgtacgt agccttctag gaaatagccg tttggcatta ctttttttaa
     8221 catgtcatgg tatttcgaaa catctatatc aattgtaatc gttttagttt gccctggttc
     8281 aatccgtatc gttttccctg taaatgtacc aagttgttga ggagccaatg tgacaaagcc
     8341 atctttaact tgatcagtat ttaaataggt tgtatagtgt aaatcttttg caactttatt
     8401 gatattatga acagtaacat caaaggaaat tttatcttta atatttccta atgtaacgct
     8461 accatagttg ttttccccac cagttaggta taatcctgtc tgaactgctg ctgcgacatc
     8521 gataatacct gctccttgtt ggcgtggtga ggtgtaagcg cctgtatctt tgtttaggtg
     8581 tgctttagca gtactcataa gaaggtattt gacagttctt tcaatgtcac cttttttaag
     8641 ttctggatgt tctttcaata agtattgttt aactagagca gtagcaccag cgacatgggg
     8701 agaagccata cttgtcccac tcatcatatc atattcatta tcattgatag cggcgtaaat
     8761 agagcctcca ggagcagata agtcaggttt taattgtccg tctgctgtta atccccagct
     8821 tgaaaagtca gataggacac ctgcattagg gttggcttgt ttatttttga tattgttaaa
     8881 tacaatttta tagttgtttt tagctaaaac atctccaaac tctttttgag taaaaatggc
     8941 aggaatagcg cttgcttcag gatctaaact cattgttaga ttagcttctc cagctttgtt
     9001 attaaagata atagctccca cagcaccgtg attaatagcg ttgacgactt tttttgtaaa
     9061 agtaatatct ccacgttcaa ttaaggcgat tttaccattt aaagttttgt ccttataatc
     9121 attgtcgttt ccttttccta cgaaaacata atcatattgc ttccctactt caaaggtctt
     9181 atcactaact ttaggatctg cataagcagc taatccgttg ttgagatttt tgttgttttc
     9241 taatccgata atgttgaaga ctttattaac cagtgttgta ttattatatg atgctacaga
     9301 aatggcctca cgagctgttg atggactacc aactaaacca taatcaggat aaagagcaga
     9361 aggctttgat gctccactcc caaatgtacc gtcgttacct gctgctataa caacagaaac
     9421 gccagcgagt ctagccatct ctaaagcttt tataagtcgg tcatcggcat taactaaaga
     9481 gccattagct ccacctaaac ttaaattaat gctatcagca ccgagtttaa cggcatcttc
     9541 aatagcttta acataaaggg ctggtccagt tcctcttttt tcatcagaga agactctcat
     9601 aaacattact tgtgcttcag gagcaacacc atagattaat tcatttgtat ctttcttgct
     9661 aggattagct gttgcgatac tggttacgtg cataccatga gaagtgcttt tcacctcttt
     9721 tagctctgta ttgacatcaa catagttgtg accaaagatt actttgttgt tataccattt
     9781 tccatagttt ataccagctt ttgctttagc agcattcatt tgctgttcgt tttggtattt
     9841 tgcttttgtc gaatcattta attggagagc atcgtgattg gtatctagtc ctgagtcaat
     9901 aattgctaca acagttccct ctcctttgta gcctgtgttc catactttgg ggacagtgat
     9961 aattttatta ctatcaatgt tttgaggggt ttctttagaa atagctggtg taggtttgac
    10021 tgctaaattt tgtttggtta aaggagtttt agaaacttct tgagtgcttt caaccggttg
    10081 atttgcggct ggggaaaatg tctcggtggc ttgagagtga ggctcagtta ctggttgtgt
    10141 ttctgttgga ctattattat tagcctttgt aacaagattg tcttgagctg ttgacgcaag
    10201 ggctgatgac gtgttgttag taggtgtctc atttgctatg ctcgttggac tgtcttttac
    10261 ttgctgttca actgcaatat cagatggctt atttatgaca gaatcagcag ctacatttgt
    10321 gataccgcct aaaaaaaata gggttcccaa aagtacagat acggcaccta acttatattt
    10381 ccggattgaa aaacgctgtt ttg
//
LOCUS       DATRQA010000065        10302 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.065, whole genome shotgun sequence.
ACCESSION   DATRQA010000065 DATRQA010000000
VERSION     DATRQA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10302
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.065"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..374)
                     /locus_tag="VBD27_001226"
     CDS             complement(<1..374)
                     /locus_tag="VBD27_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 family protein"
                     /protein_id="HEO7025237.1"
                     /translation="MKSTILVTGYKNFELGIFQDKDPRITIIKKAIDKDFRRFLENGA
                     DWFIFMGNLGFEYWALEVALDLQKEYDFQIATIFTFENHGQNWNEANKAKLALFKQVD
                     FVKYTFPSYENPGQFKQYNHFL"
     gene            449..1048
                     /gene="recU"
                     /locus_tag="VBD27_001227"
     CDS             449..1048
                     /gene="recU"
                     /locus_tag="VBD27_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEO7025238.1"
                     /translation="MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLS
                     HELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVYKGYYIDFEAKETR
                     QKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPANELISFYQIDKGN
                     KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN"
     gene            1035..3275
                     /locus_tag="VBD27_001228"
     CDS             1035..3275
                     /locus_tag="VBD27_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7025239.1"
                     /translation="MITIKKESVIKLLKYAFGIIMGFIILAIVIGGLLFAYYVSRSPK
                     LTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSADSIPLNLVNAITSIEDKRFFKHR
                     GVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLALQM
                     ERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQ
                     YDPYKNPESAQTRRNTVLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMD
                     NYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQKQLYDIYNSDTYIAYPNNELQIAS
                     TIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSGVYNS
                     TGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKS
                     FLEKLGIYYPEMNYSNAISSNNNSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKI
                     EFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVLTFGTGTKAAIPGVAQAGKTGTSNY
                     TEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGSQLDIA
                     TEVYRTMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQS
                     SQSSSSTSSEKQKEDKNTADDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNNN
                     TAPNGN"
     gene            complement(3322..4656)
                     /gene="pepC"
                     /locus_tag="VBD27_001229"
     CDS             complement(3322..4656)
                     /gene="pepC"
                     /locus_tag="VBD27_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEO7025240.1"
                     /translation="MSELTQTFTDKLLADYQANTKFSAIENAVTHNGLLKSLETRQSE
                     IENDYVFSIDLTKDEVSNQKQSGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFMEQIIATANQELSSRKVKFLLDVPQQDGGQWDMVVALFEKYGVVPKTVYP
                     ESVSSSASRELNQYLNKLLRQDAQILRELIAQGADGATVQNKKEELLQEIFNFLAMNL
                     GLPPQSFDFAYRDKDNHYQSDKNITPKAFYQKYVNLDISDYVSIINAPTVDKPYGQSY
                     TVEMLGNVVGGPAVKYLNLDMKRFKELAIAQMKSGETVWFGSDVGQVSNRQKGILATT
                     TYDFNSSMDIKLSQDKAGRLDYSESLMTHAMVLTGVDLDESGQPLKWKVENSWGEKVG
                     KDGYFVASDAWMDEYTYQIVVRKELLTKEELEAYNAEPITLAPWDPMGALAN"
     gene            complement(4769..5590)
                     /gene="nadE"
                     /locus_tag="VBD27_001230"
     CDS             complement(4769..5590)
                     /gene="nadE"
                     /locus_tag="VBD27_001230"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEO7025241.1"
                     /translation="MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYV
                     LGISGGQDSTLAGRLAQLAVEELRADTGENYQFIAIRLPYGIQADEEDAQKALDFIKP
                     DIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAGAVIGTD
                     HAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKDLYEKIPTADLEENKP
                     GIADEIALGVTYQEIDAYLEGKVVSDKSRGIIENWWYKGQHKRHLPITIFDDFWK"
     gene            complement(5587..7047)
                     /locus_tag="VBD27_001231"
     CDS             complement(5587..7047)
                     /locus_tag="VBD27_001231"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO7025242.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFNKGIHNKKAVFEAYFRKVPFE
                     NGYAVFAGLERIVRYLENLSFSDSDLSYLEELGYPEEFLDYLKNLKMELTVKSAKEGD
                     LVFANEPLVQIEGPLAQCQLVETAILNIINYQTLVATKAARIRSVIEDEPLLEFGTRR
                     AQEMDAAIWGTRAAIIGGANATSNVRAGKIFNIPVSGTHAHALVQTYGDDYQAFKAYA
                     ETHKDCVFLVDTYDTLRVGVPNAIRVAKEMGEKINFLGVRLDSGDLAYLSKKVRQQLD
                     DAGFPNAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     TDAGSMRDTIKLSNNAEKVSTPGKKQVWRITSRAKGKSEGDYITFADTDVTQLDEIDM
                     FHPTYTYINKTVRDFDAVPLLVDIFDKGKLVYQLPSLQEIQEYGRKEFDQLWDEYKRV
                     LNPQDYPVDLARDVWQNKMDLIDRIRKEALAKGEVR"
     gene            complement(7205..8119)
                     /gene="trxB"
                     /locus_tag="VBD27_001232"
     CDS             complement(7205..8119)
                     /gene="trxB"
                     /locus_tag="VBD27_001232"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO7025243.1"
                     /translation="MYDTLIIGSGPGGMTAALYAARSNLKVGLIEQGAPGGQMNNTAE
                     IENYPGYDHISGPELSMKMYEPLEKFEVEHIYGIVQRVENDGDVKRVITEDESYEAKT
                     VILATGAKNSLLGVPGEEEYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAVFLT
                     QFAKSVTIIHRRDQLRAQKVLQDRAFANEKIKFVWDSVVKEIKGNEIKVSGVTVENLK
                     TGEISEMTFGGVFIYVGLKPHSSMVSELGITDETGWVLTDTNMKTSIPGLYAIGDVRQ
                     KDLRQIATAVGEGAIAGQGVYNYITEHF"
     gene            complement(8188..8412)
                     /locus_tag="VBD27_001233"
     CDS             complement(8188..8412)
                     /locus_tag="VBD27_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEO7025244.1"
                     /translation="MFLEIAKVYLQGLGLASITVILVGIFWVFWRAKKNIDKTARERQ
                     SVLYDVLLIALMTAPILAFAFVAIILMIKA"
     gene            complement(8534..9277)
                     /locus_tag="VBD27_001234"
     CDS             complement(8534..9277)
                     /locus_tag="VBD27_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7025245.1"
                     /translation="MIKLRQLTKSFSGQKVLDKLDLDIEKGQVVALVGASGAGKSTFL
                     RSMNYLEEPDYGTIEIDDFKVDFKSISKDDILTLRRKLAMVFQQFNLFERRTALDNVK
                     EGLKIVKKMSDQEATRIARDELAKVGLADREKYYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKQGQTMVLVSHDMNFVYQVADKVLFLEKGRIL
                     ESGTPEQLFNHPLEERTKEFFASYNKSYL"
     gene            complement(9277..10080)
                     /locus_tag="VBD27_001235"
     CDS             complement(9277..10080)
                     /locus_tag="VBD27_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7025246.1"
                     /translation="MNQFILTGGWSWYNNLVSQVPAGKLFSWKAVLDAIPSILERLPI
                     TLLLTVAGALFGLILALIFAVVKINRVKILYPIQALFVSFLRGTPILVQLMLSYYGIP
                     LFLKFLNQKYGFDWNINAIPASVFAITAFAFNEAAYTSETIRAAILSVDQGEIEAARS
                     LGMTSAQVYRRVIIPNAAVVATPTLINTLIGLTKGTSLAFNAGIVEMFAQAQIMGGSD
                     YRYFERYISVALVYWAVSFLIEQLGNAIERKMAIKAPHHLTDEIPGGVR"
     gene            complement(10175..>10302)
                     /locus_tag="VBD27_001236"
     CDS             complement(10175..>10302)
                     /locus_tag="VBD27_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7025247.1"
                     /translation="KKGKTLQKFINKRIKVLKEDGTLARLSKQYFGGDYVSNIDK"
BASE COUNT         3303 a         2087 c         1631 g         3281 t
ORIGIN      
        1 atgagaaaat gattgtattg tttaaattga cccgggtttt catagcttgg gaaagtatat
       61 ttaacaaagt caacttgctt aaagagtgcc aacttagctt tattagcctc attccaattt
      121 tggccatggt tctcaaaggt aaaaattgtc gcaatttgaa aatcatattc tttttgtaaa
      181 tcaagagcaa cttccaaagc ccagtactca aaccctaaat ttcccataaa aataaaccag
      241 tctgctccat tttccaagaa gcgtctaaaa tccttatcaa tagccttttt tataatagta
      301 atccgaggat ccttatcctg gaaaatccct aattcaaaat ttttgtaacc tgttacaaga
      361 atagttgact tcatttgttg tccatcctaa ttttatggta taatagtttt gcttatctta
      421 gcattttatt tgtaatgcaa ggagaaaaat ggttaactat ccacatcagc ttattcggaa
      481 aacaacagtt acaaaatcaa agaaaaagaa aatcgatttt gccaaccgtg gtatgtcatt
      541 tgaagctgct atcaatgcaa ccaatgacta ctatttgtct cacgaattag ctgtcattca
      601 taagaaacca acgccagtac aaatcgtcaa ggtagattat cctaaacgta gtcgtgccaa
      661 gattgtagaa gcttatttta gacaagcttc tactactgat tattctggtg tttacaaagg
      721 ttactatatt gactttgaag ccaaagaaac ccggcagaaa actgctatgc ctatgaaaaa
      781 ttttcatgct caccaaatag agcacatggc aaatgtatta cagcaaaaag ggatttgctt
      841 tgtcttgctt catttttcca cacttaagga aacctatcta ctccctgcta atgagttaat
      901 ttcattttat cagattgata aaggcaataa atcaatgcct attgattata tcagaaaaaa
      961 tggatttttc gtaaaggaga gtgcctttcc tcaagtccct tacttagata ttattgaaga
     1021 aaaattatta ggcggtgatt acaattaaaa aggaatctgt tatcaaacta ttgaagtatg
     1081 cttttggcat tataatggga tttattatct tagctattgt aataggtggg ctcctatttg
     1141 catactacgt tagtcgttct ccgaaattaa ccgatcaagc tttaaaatcc gttaactcta
     1201 gtttggttta tgatggtaat aataaactta ttgccgattt aggctcagaa aagcgtgaaa
     1261 gtgttagtgc ggatagcatt ccactaaatt tggttaacgc tatcacttct atagaagata
     1321 aacgtttctt taaacataga ggtgtcgata tttatcgtat tttaggtgca gcttggcata
     1381 accttgttag tagtaatacg caaggtggtt caacccttga tcaacagttg attaaactgg
     1441 cttacttttc taccaataaa tctgaccaaa cgttaaaacg taaatcacag gaagtttggc
     1501 ttgcgcttca aatggagcgt aaatacacca aagaagaaat tcttactttc tatattaata
     1561 aagtttatat gggaaatggg aattatggta tgagaacaac agctaaatca tactttggta
     1621 aagacctaaa ggaattatct attgcacaac ttgctttgct cgctggtatt cctcaagcac
     1681 ctacacaata tgacccttat aaaaacccag aatctgctca aacaagacgt aataccgttc
     1741 ttcagcagat gtatcaagat aaaaacattt ctaaaaagga atacgaccaa gctgttgcaa
     1801 ctccagtaac tgatggctta aaagaattaa agcaaaaatc tacttatcca aaatatatgg
     1861 ataactactt aaaacaagtt attagtgaag ttaaacaaaa aactggtaaa gatatcttta
     1921 ctgctgggct aaaagtgtat actaatatca acactgatgc acaaaaacaa ctatatgaca
     1981 tctacaacag tgatacttac atcgcttatc caaacaatga attacaaata gcatctacca
     2041 tcatggatgc gactaatggt aaagtcattg cacaattagg cgggcgtcat cagaatgaaa
     2101 atatttcatt tgggacaaat caatctgtct taacagaccg cgattggggt tctacaatga
     2161 aacctatctc agcttatgca cctgctattg atagtggtgt ctataattca acaggtcaat
     2221 cattaaacga ctcagtttac tactggcctg gtacttctac tcaactatat gactgggatc
     2281 gtcaatatat gggttggatg agtatgcaga ccgctattca acaatcacgt aacgtccctg
     2341 ctgtcagagc acttgaagcc gctggattag acgaagcaaa atctttcctt gaaaaattag
     2401 gcatatacta tccagaaatg aactattcaa atgctatttc aagtaacaac aatagcagtg
     2461 atgcaaaata tggtgcaagt agtgagaaaa tggcagcggc ttactcggct tttgcaaacg
     2521 gcggaactta ctataagccg caatatgtta ataaaattga atttagcgat ggaaccaatg
     2581 atacttatgc agcgtctggt agccgtgcga tgaaagagac tactgcctac atgatgacgg
     2641 atatgttgaa aacagtacta acatttggta ctggtactaa agcagctatc cctggtgttg
     2701 cacaagctgg taagactggt acttccaact atacagaaga tgagttagct aaaattgaag
     2761 caactactgg tatctacaat agcgccgttg gtacaatggc tcctgacgaa aactttgtcg
     2821 gctatacttc taagtacaca atggcaattt ggactggtta taaaaatcgc cttacaccac
     2881 tttatggtag ccaactggat attgctactg aggtttatcg tacaatgatg tcctacctaa
     2941 ctggcggata cagtgcagat tggacaatgc cagaagggct ctatcgaagt ggaagctact
     3001 tgtatatcaa tgggacaact acaaccggca cttactcttc ttctgtctat aagaatatct
     3061 atcaaaattc tggacagtca agtcaaagca gtagttcaac atcctctgag aaacaaaaag
     3121 aggataagaa tacagcagat gatgccaatt catcatcccc acaagtcgaa acacctaata
     3181 atggtaatgc tactacccct aataattcca atcaaactgt gccaggaact ggacatggta
     3241 atggtaataa caatacagca cctaacggta attaaaaaaa ctggataata tatccagttt
     3301 tttaatttat aattactatt actaattagc tagtgctccc atcggatccc acggcgctaa
     3361 agtaattggc tctgcgttgt aagcctctaa ttcttcttta gtaagcaatt ctttacgaac
     3421 gactatttgg taagtatatt catccatcca agcatctgaa gctacaaaat aaccatcctt
     3481 accaactttc tctccccaag agttctcaac tttccatttt aaaggttgtc cagattcatc
     3541 aaggtctact cccgttaaga ccatagcatg tgtcattaaa ctttcactat aatctaggcg
     3601 gcccgctttg tcttggctaa gtttaatatc catagatgaa ttaaaatcat aagtcgtagt
     3661 tgctaggata cccttttgac gatttgatac ttgacctacg tctgaaccaa accacacagt
     3721 ttctcctgat ttcatttgcg caatcgctaa ctctttaaac cgtttcatat caaggtttaa
     3781 atatttaaca gctgggcctc cgacaacatt tccaagcatc tcaactgtat aggattgtcc
     3841 ataaggctta tctacagttg gagcattgat aatagaaaca taatctgata tgtcaagatt
     3901 aacatatttt tgataaaatg ctttcggtgt aatattttta tcagactgat aatgattgtc
     3961 tttatcacga taagcaaaat caaaagattg cggtggcaag ccaaggttca ttgctaaaaa
     4021 gttaaaaatt tcttgaagca attcttcctt tttattttga acagttgccc catctgctcc
     4081 ctgcgctatt aattctcgta gaatctgtgc atcttggcgt aaaagtttat tcaaatattg
     4141 atttagttca cgactagcac ttgatgaaac agattcagga taaactgttt taggaacgac
     4201 accatatttc tcaaacagag caaccaccat gtcccattgt ccgccatctt gttgaggtac
     4261 atctaatagg aattttacct tacgactact caattcctga tttgcagtcg caataatttg
     4321 ttccataaac caatttgatt tttcatactt atcccagaaa aatgtatgtg cctgagataa
     4381 ttcaaagttc tcgagtttaa aatctgaaat cagtttatga cgaaatgtgt tcagtgcagc
     4441 aaacatccag caacgtcctg attgtttctg attagagact tcatctttgg tcaaatcaat
     4501 tgaaaagaca tagtcgttct caatctcact ttgacgagtc tctagagact tcaataaacc
     4561 attgtgagtt actgcatttt caatagcgct aaattttgta tttgcttggt aatcagctaa
     4621 taacttatct gtaaatgttt gagttaattc tgacataaat tcctcctttt atacttagct
     4681 actattataa acattctgaa tatttctgca agtctgaaag taatttatta gagaaacaaa
     4741 aatagctaga atattctagc tatttttctt atttccagaa atcatcaaaa attgtgattg
     4801 gaagatgacg tttatgttga cccttatacc accaattttc aatgatacct cgagacttgt
     4861 ccgaaactac cttcccttca agataagcat caatttcttg gtaggttact ccaagtgcta
     4921 tttcatcagc gattcctggt ttattttctt ctaaatctgc ggttggaatc ttttcgtaca
     4981 agtctttatc agcaccaagt tcagctaata actgttttcc ctgactttta ttaagtctaa
     5041 agagaggtaa taagtccgca cctccatcac caaatttggt gaaaaaacct gtaatatttt
     5101 ctgcagcatg atcagtacca ataaccgctc cagcatattg acctgcaact gcatattgac
     5161 taatcattct ttgacgagct ttaatatttc ctttattaaa gtctgtaatc tctactcctg
     5221 ctgcattaag agctctaact tggccatcaa cagcttcttt aatattaatg gtcaaagcaa
     5281 tatctggctt gataaaatct aatgcttttt gtgcatcttc ttcatcggcc tgaataccat
     5341 atggtaaacg aatagctata aattgatagt tttcccctgt atcagcacgc aactcttcaa
     5401 ctgctagttg agctagacgt cctgctaaag ttgaatcttg cccccctgaa atacccagta
     5461 cataggtctt tagaaaacta tgttttaata gatagtcttt taaaaattct acggaacgac
     5521 ggatttcttg acttggatta ataacaggtt ttacaccgag ttctttgata atttgatctt
     5581 gcaaagtcat cttacttctc cttttgccaa agcttcttta cggatacgat caatcaggtc
     5641 cattttattt tgccaaacat cgcgtgccaa atcaactggg taatcctgtg gattcagaac
     5701 acgtttatac tcatcccaaa gctgatcaaa ttctttgcgt ccgtactctt gaatttcttg
     5761 aaggcttggt aattgataaa ctaatttccc tttgtcaaag atatctacca gtaagggtac
     5821 tgcgtcaaaa tcacggactg ttttattgat ataagtataa gttggatgaa acatgtcaat
     5881 ttcatctaat tgtgtcacat cagtgtctgc aaatgtgatg taatcaccct cagatttccc
     5941 tttagctcga ctggtgatac gccaaacttg ctttttccca ggtgttgata ctttttccgc
     6001 attgtttgat agtttaattg tatctcgcat actaccggcg tcagtctcaa tagaaacaat
     6061 tttataaaca gcacctagcg caggctgatc ataggctgta attaattttg ttccgacacc
     6121 ccaaacatca attttagctt tttgcatttt taggttgagg attgtatttt catctagatc
     6181 attcgaagca taaatctttg cattaggaaa accggcgtca tctaactgct gacgaacttt
     6241 cttagacaaa taagctaaat cacctgaatc aaggcgaact cccaagaaat taatcttttc
     6301 gcccatctct tttgcaactc gaatagcatt aggcacacct acacgaaggg tatcataagt
     6361 atccactaaa aagacgcaat ctttatgagt ttctgcgtaa gctttaaatg cctggtaatc
     6421 atcgccataa gtttgaacta aagcatgtgc atgcgttcct gatactggaa tattaaagat
     6481 tttaccagca cgaacattac tcgttgcgtt agcaccacca ataatcgctg ccctagtacc
     6541 ccaaatggct gcatccattt cttgagcacg tcgtgtccca aactctaata gcggttcatc
     6601 ttcaataaca gaacgaatac gagctgcttt tgtagcaaca agtgtctggt agttgatgat
     6661 atttaaaatc gctgtctcta ctaactgaca ttgtgctaaa ggaccttcaa tttgaactaa
     6721 tggttcatta gcaaatacaa ggtcaccttc cttagctgat ttgacagtca gttccatttt
     6781 taaatttttt aagtaatcta aaaattcttc aggataacct aactcttcta aataggataa
     6841 atcactatca gaaaagctca gattttctaa ataacgaaca atacgttcta aacctgcaaa
     6901 aacagcataa ccattttcaa atggaacctt gcgaaagtaa gcttcaaaca ccgctttttt
     6961 attgtgaata cctttattaa aatagacttg catcatatta atttgatata aatcagtatg
     7021 tagtgttaaa ctatcgtctt tatacatcag acaatctcct tttaatcatg agcctaggat
     7081 aaggctatat gtctaacatt atagcatatt tctatctcgg tagttcagtt tatcacctta
     7141 ctttcatgaa aataaaaaga aagactaatc gtttttcgat agtcttttct acttttagta
     7201 tttcttagaa atgctcagtt atatagttat aaacaccctg tccagcgata gcaccttcac
     7261 caacagcagt cgcaatctga cgtagatctt tttgacgaac atccccaata gcatatagtc
     7321 ctggaattga tgttttcata tttgtatcag ttaagaccca gccagtctcg tccgtaatac
     7381 ctaattctga gaccatgcta gaatgtggtt tcaaaccaac gtaaatgaaa acacctccga
     7441 aagtcatctc tgagatttct ccagtcttta gattttcaac agttacacca gatactttaa
     7501 tctcatttcc tttaatctct ttgaccactg aatcccatac aaattttatt ttttcgttag
     7561 caaaagctcg gtcttgaagt actttttgag ctctcaattg atcacgtcgg tgaataatag
     7621 taacactttt agcaaattga gttaagaaga cagcttcttc aacagcagaa tcaccgccac
     7681 ccacaactaa aagatcctga tcacggaaaa atgcaccatc acaaaccgca cagtaagaaa
     7741 cgccacgact agtatattcc tcttcacccg gaactcctaa aaggctgttt ttagctccgg
     7801 tagcaaggat tactgttttg gcttcatacg attcatcttc ggtgataaca cgctttacat
     7861 caccatcgtt ctcaactctt tgaactatac cataaatatg ctctacttca aatttttcca
     7921 acggctcata catcttcatt gataattctg gacctgagat atgatcgtag cctggatagt
     7981 tctcaatttc agcagtatta ttcatctgtc cacctggagc accttgttca attaacccaa
     8041 ctttaaggtt tgaacgtgca gcgtaaagcg ctgctgtcat accaccagga cctgaaccta
     8101 ttattaaagt atcatacatt actatttctc ctttgaatct tatggtttta ttctatagca
     8161 aagcgtctat atttccaact aatctgacta agcttttatc attaaaatga tagctacaaa
     8221 tgcaaaggca agtattggag ctgtcataag agctatcaga aggacatcat aaagaacaga
     8281 ttggcgttct ctagctgttt tatctatgtt tttcttggca cgccaaaaga cccaaaatat
     8341 accaactaaa ataacagtta tgctggcaag ccctaaacct tgtaaataaa cttttgctat
     8401 ttctaaaaac atctctatcc aacaagcgat tgtcaaaaca tatcgcagct ttcttatata
     8461 cttagcctaa ctcaagaata attaaaaatg aagattagac attctaacct tcattatatc
     8521 aaaatatttt agcttatagg tacgatttgt tataactagc gaagaattct tttgtacgtt
     8581 cttctaaagg atggttaaat aattgttcag gcgtcccaga ctctaaaata cgaccttttt
     8641 ccaaaaataa aactttatct gctacttggt aaacaaaatt catatcatga ctaacaagca
     8701 ccatggtttg cccctgctta gcagcatctg caatagattt ttcaacttct ccaactaatt
     8761 ctggatcaag tgctgatgtc ggttcgtcta aaagaagaac atcaggtttc atggctaatg
     8821 cgcgtgccaa agcaacacgt tgtttctgtc cacctgataa atgtcttgga taatattttt
     8881 cacgatctgc aagtccgacc tttgccagtt cgtctcttgc aatccgtgtt gcctcttgat
     8941 ctgacatctt tttgacaatt ttcaaacctt cttttacatt atccaaagct gtacgacgct
     9001 caaatagatt gaattgttga aaaaccatgg ctaacttacg gcgtaatgtt agtatgtcat
     9061 ctttactgat tgatttaaaa tcaaccttaa aatcatcaat ttcaattgtt ccataatcag
     9121 gctcttctaa ataattcata ctacgaagaa aggttgattt tcctgcacct gaagcaccta
     9181 ctagagcaac aacttgacct ttttcaatat ctaaatcaag tttgtctaac actttttgac
     9241 cagaaaatga tttagtcaat tgtctcaatt ttatcattag cgtactcctc ctggaatctc
     9301 atcagttaaa tggtgtggag ccttaattgc catttttcgt tcaatagcat ttcccaattg
     9361 ttcaattaag aaactaaccg cccagtaaac aagtgccaca gagatataac gttcaaaata
     9421 gcgataatcg ctaccaccca taatttgtgc ttgggcaaac atttcaacaa taccagcatt
     9481 aaaagctaaa gaggttccct tagttagacc aattaacgtg tttatcaaag taggtgttgc
     9541 gactactgct gcgtttggaa taataacacg acgataaact tgcgctgagg tcatccctaa
     9601 actacgtgct gcctcaattt caccttgatc aaccgacaaa atcgccgctc gtatagtttc
     9661 acttgtataa gccgcttcat taaaagcaaa tgcagtaata gcaaatacag aagccggtat
     9721 agcattaatg ttccaatcaa aaccatattt ttggtttaaa aatttcaaga aaagtggaat
     9781 tccataataa ctcagcatta attgaacaag tattggagtc ccacgtaaaa aactaacaaa
     9841 taaagcttga atcggatata aaattttgac acggttaatt ttcactactg caaaaatcaa
     9901 agctaaaatg agtccaaata aggctcctgc tactgtcaat aatagcgtta tgggtaatcg
     9961 ctctaaaatt gagggtattg catctaaaac agccttccaa ctaaataatt tccccgctgg
    10021 aacctgacta accaaattat tataccaaga ccatccaccc gtcaagataa attgattcat
    10081 tccttctact ttccttttct aaactttctt atcttcgtta ttctatcacg caaactctca
    10141 catgtaaaaa gataaaactt atagtctcac tgatttattt atcaatgttt gaaacgtaat
    10201 ctccaccgaa atattgttta cttaaacgtg ccaaagtacc atcttctttc aaaactttaa
    10261 tacgcttatt tataaatttc tgtagagttt tacctttttt at
//
LOCUS       DATRQA010000066        10217 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.066, whole genome shotgun sequence.
ACCESSION   DATRQA010000066 DATRQA010000000
VERSION     DATRQA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.066"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            47..1584
                     /locus_tag="VBD27_001237"
                     /pseudo
     CDS             47..1584
                     /locus_tag="VBD27_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            1761..3305
                     /locus_tag="VBD27_001238"
     CDS             1761..3305
                     /locus_tag="VBD27_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO7025248.1"
                     /translation="MTLQDEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLEELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VIDPLAKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWINSDDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     KLEEKM"
     gene            3391..3771
                     /locus_tag="VBD27_001239"
     CDS             3391..3771
                     /locus_tag="VBD27_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO7025249.1"
                     /translation="MGIFSGLMGNASQVDTDKVENQLSDILIPGEQVDLAYTLIRDLI
                     VFTNYRLILVDKQGVTGKKVSYNSIPYASISRFTVETSGHFDLDAELKIWISSAIEPA
                     EVLQFKNDRNIVSIQKALAIAVLK"
     gene            3965..4699
                     /locus_tag="VBD27_001240"
     CDS             3965..4699
                     /locus_tag="VBD27_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7025250.1"
                     /translation="MIIELKSVGKIFPKSQFRLDDISFNIAEGEIVGLIGSNGTGKST
                     ILKMINGLIPYDKGNIYYQGKEVKSFSDNKLRQMRKDIAYIFQNHNLLAGESVYYHLA
                     LVYKLNHQKVNHDAINDILDFLGLMDLKQVKCHSLSGGQQQKVAIAMAVLQKPKLILC
                     DEISSALDTNSEKEIFNLLSDLREKYGISILMIAHHLSLLKQYCDRVMILDHQTIVDT
                     VVPVKATLNQLKSNYVDQVKEFLLHD"
     gene            4692..5354
                     /locus_tag="VBD27_001241"
     CDS             4692..5354
                     /locus_tag="VBD27_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025251.1"
                     /translation="MIDIIEKHGEGILKSFWETNLMLGLTLILCFLIAFPTGILLFSL
                     RKSYLIKHSLAYQLLNLFLGTLRSVPFLIFIFILIPLNRLIFGTSFGTIAAILPLTLV
                     SVSLYARYVEQALLNIPQVVVDRALSLGANKRQIIYYFLIPSIKIDLVLSFTATAISI
                     LGYSTIMGVIGAGGLGEYAYRFGYQEYDYPVMYLIVVLFIIYVFILQSLGYFIANRYS
                     RK"
     gene            5370..6200
                     /locus_tag="VBD27_001242"
     CDS             5370..6200
                     /locus_tag="VBD27_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025252.1"
                     /translation="MKKLLSLTCLIMMSLCLVACTKQAMSSKQIKDKNSKEKVITVAT
                     YSKPTSTFLDLIKDNVKEKGYTLKVVMVSDYIQANIALENKEHDANLLQHEFFMSIFN
                     KENDGHLVSITPIYHSLAGFYGQHLKNIAELKDGAKVAIPSDPANMTRALLLLQEKKL
                     ITLKNTSKKTKAIEDIITNPKKLRIEPVALLNLNQAYFEYDLVFNFPGYVTKINLVPK
                     RDRLLYEKKPDIRFAGALVAREDNKNSDKIKVLKEVLTSKEIRHYITKEIPSEADVAF
                     "
     gene            6435..8021
                     /locus_tag="VBD27_001243"
     CDS             6435..8021
                     /locus_tag="VBD27_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7025253.1"
                     /translation="MKFTELNLSQDILSAVEKAGFVEPSPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIHTEDNTIQALIIAPTRELAVQSQEELFRFGRDKGVKVRS
                     VYGGSSIEKQIKALRSGAHVVVGTPGRLLDLIKRKALKLNHIETLILDEADEMLNMGF
                     LEDIEAIISRVPEARQTLLFSATMPDPIKRIGVKFMKDPEHVKIKATELTNVNVDQYY
                     VRVKENEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDHIDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLTIIENLTKKRMTGMKPATASEAFQAKKKVALKRIARDFEDQ
                     ELVSKFDKFKADALELATQYTPEELALYVLSLTVQDPESLPEVEITREKPLPFKPSGG
                     GFKGKGGRGNGRGGDRRRNDRGDRRGNRDRDDRGGRRDFKRRDDKFKKDNRRQENKKP
                     HKNTSSEKQTGFVIRNKGDK"
     gene            complement(8204..8470)
                     /locus_tag="VBD27_001244"
     CDS             complement(8204..8470)
                     /locus_tag="VBD27_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO7025254.1"
                     /translation="MIKVPAYMYVLECSDGTLYTGYTTDVKRRLNTHNTGKGAKYTRA
                     RLPVKLLYSEAFNSKQEAMRAEALFKQKTRKAKLTYIKQHKNEQ"
     gene            complement(8463..9227)
                     /locus_tag="VBD27_001245"
     CDS             complement(8463..9227)
                     /locus_tag="VBD27_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEO7025255.1"
                     /translation="MIDTILKENERIDQLFSTDVKIIQNKEVFSYSIDSVLLSRFPKL
                     PSRGLIVDLCSGNGAVGLFASTKTSATIIEIELQERLADMAKRSIKLNKLEKQVTMIN
                     DDLKNLLDHVQRSNVDLMLCNPPYFKASETSKKNLSPHYLLARHEITTNLREICQIAQ
                     HALKTKGRIAMVHRPDRFLEIIDTMHQFNLAPKRIQFVYPKLGKDANMLLIEAIKDGS
                     TEGMKILPPLVVHQDNGDYTETIFDIYFGENGKSHD"
     gene            9362..10102
                     /locus_tag="VBD27_001246"
     CDS             9362..10102
                     /locus_tag="VBD27_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO7025256.1"
                     /translation="MFYTYLRTLVMFLIWVANGNAHYHNEDKMLKDDENYILVAPHRT
                     FWDPVYMAFAARPKQFVFMAKKELFTNRLFGWWIKMCGAFPIDREKPGQDAIRYPVKM
                     LKNSNRSLVMFPSGSRHSKDVKGGVAVIAKMAKVRIMPAAYRGPMVFKNLLKGHRVDM
                     NFGNPIDVSDIKRMDAEGIAEVSRRIQEEFDRLDRENETYDDGKKLNPLTYIYRLPLA
                     IIAIVLLVLTLIFSYLASFVWDPQKHLK"
BASE COUNT         3359 a         1561 c         2001 g         3296 t
ORIGIN      
        1 cggttaatta ttttttgaaa tatatttttt ctggaggaaa atagtaatga ataaatcatt
       61 caataccaaa ttaggtttgg taactgtagc ggcattatca ggtattgtat taacgtctca
      121 attacctgtg aacgctaaag ctgagacacc agtaatggca gcaagtgcac aacaaggttt
      181 ccgttttgta gcaaccgttg tagatagtca gacgcgtgtc ttgccgggaa aattggtaac
      241 tttgtcagag gtgacaactg gtcagcctaa aattattgca agtgtaaaaa cgaatgatgc
      301 cggacaagct atttttaata atctccctat aaaacgtaac cttagtgtgt ctgttgatgg
      361 acaagtaaaa ggatatacta ttcgaactga cgtagcaggc tctagtaaag cagctagttt
      421 tacagcaaca ggagtaggga caaatgagcc aacgtattca aaaaaaacca tagacattac
      481 agtgagagac caaaatgctg aacctgtttc aggcaggaca gttaccttaa aaacacatgc
      541 tggtcgtgag attgctagct tggtttctgg tgacaatgga ttaactcgct ttactgatcg
      601 tttactagat ggaacattct accaatattt tgttgatggg aaaaaattgg tgatattgta
      661 cctggagaat caagaagtgc atatgttaat gttgcaccta aaaaagatta ttttaccttt
      721 acagtcactg ctttagataa agatggactt gttgttaagg gaaaagaagt aacgttgact
      781 gatattactg atggtaaagc agtagcgctt gcttctttaa aaacgaatga taatggacaa
      841 gctatcttta ccaacctacc attatcaaga aatatcagtg tttcaattga tggaaaatca
      901 aagggctata ctcttcgaac tgatgtagca ggctctcata aagcggctgc attctatgta
      961 gatggtaaag gtaccaaagc acctcaattt agtgcagaag cagcggttgt aactatttac
     1021 gatgctaatg gaaatactat agcaaaccaa gaagtgacat taactaattc aaatgggaca
     1081 attgttgcaa aaggtttgac tgaaaaagat ggcaaagcaa ggttcgccaa taaattaatg
     1141 ggaggaacgt tatacaatat ttttgtaaat ggtattgaga tgcctaaaac tgcccttgtt
     1201 ggtagtgatg tcagtgtttt tctaacagat aaacaaatta agaaagaaaa acctgttact
     1261 cctgataaat tggataaaga aatgaaccaa gagaaggaaa aggagctctt agatactcca
     1321 aaaccagtac cacctacttc gaaaccaaca gcaccaatta aaaataaaaa ggacgattcc
     1381 cctaagaagt ttacgaaaag tcaaccttct agtttgaagt caagtgttaa ggctataggt
     1441 gctaagccaa aagctatgac agccttagct aaaaaattgc ctaaaactgg tgatcaagcc
     1501 attagtattt taactttggt tggttttatt gtaacagggt tagcgattat gttaggcttc
     1561 tttaaaaaat cacgatcaaa ataatttatt ttagaagctt gggtctttag acacaggctt
     1621 ctcttctttc ttcggaaata gattgaactt gaagaatgtt gttaaattcg ctataataat
     1681 aaagctatag acaagattat tgtttcagtt gagactactg aaaataatac taagaaatgg
     1741 ataaaagcta aggattgaat atgacattac aagacgaaat caaaaaacgc cgtacttttg
     1801 ccatcatctc tcacccggat gctggtaaga cgactattac tgagcaatta ttatattttg
     1861 gtggtgaaat tagagaagca gggacagtaa aagggaaaaa atcaggtact tttgcaaagt
     1921 ccgactggat ggatattgaa aagcaacggg gtatctctgt tacttcatct gttatgcaat
     1981 ttgattacgc gggtaaacgt gttaatattt tggatacacc tggacatgag gatttctcgg
     2041 aagatacata ccggacctta atggctgtgg atgcagctgt catggttgta gattcggcta
     2101 aaggtattga ggctcaaact aaaaaacttt ttgaagttgt taagcatcgt aatattccgg
     2161 tttttacgtt tattaataaa ttggaccgtg atggacgtga gccactggat ttgcttgaag
     2221 agttagaaga agtactaggt attgcttctt atccaatgaa ttggccaatt ggtatgggga
     2281 aatcatttga agggctttat gatttacata ataaacgttt agaactctat aaaggagacg
     2341 agcgttttgc aagtattgaa gacggtgatc agctgtttgc caataatcca ttttatgaac
     2401 aagtcaaaga agatattgaa cttcttcaag aagcaggtaa tgacttttca gaacaagcta
     2461 ttcttgatgg tgacctaact ccagtattct ttggctctgc ccttacgaac tttggagttc
     2521 agacttttct tgatactttc ttggagtttg ctccagaacc tcatggtcac aagactacag
     2581 aaggtaatgt tattgacccc ttagctaaag atttttcagg ttttgtcttt aaaattcaag
     2641 ctaacatgga cccacgtcat cgcgaccgta ttgcttttgt tcgtattgtt tctggtgaat
     2701 ttgagcgcgg catgggagtt aatttgacac gcactggtaa aggcgctaag ctctccaatg
     2761 ttactcaatt tatggcagaa tcacgagaaa atgtgacgaa tgctgttgct ggggatatta
     2821 tcggggttta tgatacagga acttatcaag ttggggatac cttaacggtt gggaaaaata
     2881 aatttgaatt tgaaccatta ccaactttta ccccagaatt atttatgaaa gtatcagcaa
     2941 aaaatgttat gaagcagaaa tcattccaca agggtattga gcaattagta caagaaggag
     3001 caatccagct ttataagaat tatcagacag gcgaatacat gctgggtgct gtgggtcaac
     3061 ttcagtttga agtttttaaa catcgtatgg aaggtgaata caatgctgaa gttgttatga
     3121 caccaatggg taaaaagact gtacgttgga ttaactcaga cgatttagac gaacgcatgt
     3181 cctcaagccg aaatatttta gcaaaagatc gttttgacca accagttttc ctatttgaaa
     3241 atgattttgc acttcgctgg tttgctgata agtatcctga tgtgaagctt gaagaaaaaa
     3301 tgtaaaattt aaagtcactc aaatttgagt gactttttaa gttttttaga ctaaaatagt
     3361 tataatacat agaaaagatt ggagattaac atgggaattt tttcgggatt aatgggaaat
     3421 gcttcacaag tggataccga taaagtcgag aatcagttat ctgatattct gattccaggt
     3481 gaacaggttg atttagctta tactttaatt cgtgatttga ttgtttttac taattatcgt
     3541 ttaattttag tcgataaaca gggggtgaca ggtaaaaagg tttcttataa ttctatacct
     3601 tatgcttcta tatcacgttt tacagtggaa actagtggtc attttgattt agatgctgag
     3661 ttaaagattt ggatatcatc agcaatagaa ccagcagaag tacttcagtt taagaacgac
     3721 cgtaacatag tgagtataca gaaagctttg gcgatagctg ttttaaaata aacaatttag
     3781 caagtgctgc tactaagagt tatttgtttt aattataact tttaatagtg ctaaattctc
     3841 attttcaaaa taaaagtagt gatttttatc actgctttta ttttgaagta tagtaggcct
     3901 caagcatata ttttgaaaac gctgtatttt tttgataaga tatttttttg aggagtatta
     3961 aataatgata atagaattaa agagtgttgg gaaaattttt ccaaaatcac agtttagatt
     4021 agatgatatt tcttttaata tagcagaagg cgagatagtt ggtctgattg gttctaatgg
     4081 tactggaaag agtactattt tgaagatgat taatggctta atcccttatg ataaaggaaa
     4141 tatttattat caaggaaaag aagtgaagtc tttttctgat aataagttac gtcagatgcg
     4201 taaagatatt gcttatattt ttcaaaatca taatttgtta gcaggagaaa gtgtatatta
     4261 tcatctagct ttagtttata aattaaatca tcaaaaagta aatcatgacg caattaatga
     4321 tatcttagat tttttaggtt tgatggattt aaaacaggtg aaatgccata gtttgagtgg
     4381 tgggcagcaa caaaaagttg caatcgctat ggctgttttg caaaaaccga aacttattct
     4441 atgtgatgag atcagctctg ccttagatac taatagtgaa aaagagatat ttaacttgtt
     4501 gtctgatctt cgtgaaaaat atggtatctc tatcttgatg attgctcatc acctatctct
     4561 tttaaaacaa tattgtgatc gtgtaatgat tttggatcac cagactattg tagatactgt
     4621 tgttccagtg aaagcaactc tgaatcaatt aaagtcaaac tacgtagatc aagtaaagga
     4681 gtttttgtta catgattgac attattgaaa aacatggcga aggtatctta aaatcatttt
     4741 gggaaaccaa tttaatgtta gggttaacct tgattctttg ttttctaatt gcttttccaa
     4801 caggtatttt attgttttca ttgagaaagt cttacttgat taaacattct ttagcctatc
     4861 aactactaaa tttattttta ggtacgttac ggagtgtccc tttccttatt tttattttta
     4921 tcttaatccc tctcaatcga ttaatttttg ggacctcatt tggaacgata gcggcaatct
     4981 tacctcttac tttggtttca gtcagtttgt atgcacgtta tgtggaacaa gctttattaa
     5041 atatacctca agttgttgta gatcgagctc tcagtctagg ggctaacaag agacagataa
     5101 tttattattt tttaatcccc tccatcaaga ttgaccttgt tttatcattt actgcaacgg
     5161 ctataagtat tcttggctac tcgacaataa tgggagttat tggtgcagga ggtttgggtg
     5221 aatatgccta tcgttttggc tatcaagagt atgactaccc agttatgtac ttgattgttg
     5281 tcttatttat aatatatgtg tttatcttac agagtttggg ctattttata gcaaaccgct
     5341 actcaagaaa ataaaagaaa agaggaacta tgaaaaaatt actttcccta acatgtctaa
     5401 tcatgatgtc tttatgttta gtggcatgta ctaagcaagc aatgtcgtct aagcaaatta
     5461 aagataagaa tagtaaagaa aaggtgatta ctgttgcaac ttacagcaaa cctacatcta
     5521 cctttttaga tttgattaaa gataatgtaa aagaaaaagg atatacttta aaggttgtca
     5581 tggtctctga ctatattcag gctaacattg ctttagaaaa caaagaacat gatgctaacc
     5641 ttttacaaca tgaatttttc atgagtatct ttaataagga aaatgatggt catctagtgt
     5701 caattacacc aatttatcat tcattggctg gtttttatgg tcaacatttg aaaaatattg
     5761 ccgagcttaa agacggtgct aaggtagcga ttccgtctga tcctgccaat atgactagag
     5821 ctctgctatt attgcaagaa aagaaactta tcaccttaaa gaatacgtcc aaaaagacca
     5881 aggctatcga agatattatt actaacccta aaaaattacg aattgaacct gtagcattac
     5941 ttaacctcaa tcaggcctat tttgaatatg accttgtctt taatttccct ggatatgtga
     6001 caaaaatcaa tctagttcct aaaagggata gattattata tgagaaaaaa ccagatatcc
     6061 gttttgcagg tgccttggta gctcgtgaag ataataaaaa tagtgataaa ataaaagtac
     6121 ttaaagaagt actaacaagt aaagagattc gtcactatat cactaaggag attccaagtg
     6181 aagcagacgt tgcgttctag atagataaac tatgaaccct ccttctttat agatggaggg
     6241 tttttattgc cttttttcta ttttctgaag caactaatag aactagcaat ttatgaaagc
     6301 gtgttattta ttagattgac cttattttat atctttttac ttttaattgt gttacactaa
     6361 gtaggttgtc agaaaagata actaataaag gttcataagg cagcttcgca ctgtctttta
     6421 gaaaagagaa acatttgaaa tttacagaac ttaacttatc gcaagatatt ctttcagcgg
     6481 tagaaaaagc aggctttgta gagccatcac caatccaaga aatgactatc ccacttgctc
     6541 ttgaaggcaa agatgtcatt ggtcaagcgc agacaggtac aggaaaaact gcagcttttg
     6601 ggttaccaac tttaaataaa attcatactg aagataatac tattcaagca cttattattg
     6661 ctcctacacg tgaacttgca gtacaatcac aagaagaatt gttccgtttt ggacgcgata
     6721 aaggtgttaa ggtccgctct gtttatggtg ggtcaagtat tgaaaaacaa atcaaggcat
     6781 tacgttcagg agcacacgtt gttgttggaa ctccaggtcg attacttgat ttaattaaac
     6841 gtaaagcact taaacttaat cacatcgaaa ctcttattct tgatgaagca gacgaaatgc
     6901 ttaacatggg atttttagag gacattgaag caatcattag tcgtgtacca gaagcacgtc
     6961 aaactctttt attctcagcg acaatgcctg atccaatcaa acgtatcggg gttaaattca
     7021 tgaaagatcc tgagcatgtt aaaattaaag caactgaatt aactaatgtg aatgttgatc
     7081 agtattatgt tcgcgttaag gaaaatgaaa aattcgatac tatgacacgt ttgatggacg
     7141 ttgatcaacc agaattgtca attgtctttg gacgtacgaa acgtcgtgta gatgaattaa
     7201 ctcgtggtct aaaattacgt ggtttccgag cagaaggaat tcatggagac ttagatcaaa
     7261 ataaacgtct tcgtgttatc agagacttta aaaatgacca tattgatatt cttgttgcca
     7321 cagatgttgc tgcgcgtggt ttagatattt caggcgttac acatgtttac aactatgata
     7381 ttccccaaga tccagaaagt tatgttcacc gtattggacg tactggtcgt gctggtaaat
     7441 ctggtcaatc tatcactttt gtttcaccaa acgaaatggg atatttgaca attattgaaa
     7501 acttaactaa aaaacgtatg actggaatga agcctgctac tgcaagcgaa gcatttcagg
     7561 ctaagaaaaa agttgcattg aaacgtattg cacgtgactt tgaggatcaa gaattagttt
     7621 ctaaatttga taaatttaaa gccgatgctt tagaattagc aactcaatat acaccagaag
     7681 aacttgctct ttatgtctta agcttaacag ttcaggatcc agaatcgtta ccagaagttg
     7741 aaatcacacg tgaaaaacca ttaccattta aaccatcagg tggtggcttt aaaggtaaag
     7801 gtggacgagg taatggccgt ggtggcgatc gtcgacgcaa tgatcgtggt gatcgtcgtg
     7861 gaaatcgtga ccgtgatgat cgtggaggtc gtcgtgattt taaacgtcgt gatgataagt
     7921 tcaagaaaga taatcgtcgt caagaaaata aaaaacctca taaaaacact tcaagtgaaa
     7981 aacaaactgg atttgttatt cgcaacaaag gcgataaata agaggattac gaaaaaggag
     8041 ttgctgagcg actccttttt tattttttaa gataaagcgt agatagtgat atccgtaaaa
     8101 ttgccttata actttaagaa tgaagacaaa agattaaagt gaggttgaaa tttaaaagtt
     8161 aaagaagcga ggacaggctc attgcactta gtatattatc gtattattgt tcatttttat
     8221 gttgtttaat ataagtcaat tttgctttcc gagtcttttg tttaaataat gcttctgccc
     8281 tcattgcttc ttgtttgcta ttaaaagctt cagaataaag aagttttact ggcaatctcg
     8341 cacgagtata tttagcacct tttccggtat tgtgtgtatt gagacggcgc ttgacatctg
     8401 tagtataacc tgtataaagt gttccatctg aacattctaa aacatacata taagcaggaa
     8461 ctttaatcat gagattttcc gttttccccg aaatatatat caaaaatagt ctcagtatag
     8521 tctccattat cttgatgaac aacaagagga ggaagtattt tcataccttc tgtcgagcca
     8581 tctttaatag cttcaattaa aagcatatta gcatcctttc ctaattttgg ataaacaaat
     8641 tggatacgtt taggtgctaa attgaactga tgcatcgtat caataatttc aaggaaacgg
     8701 tctggacgat gtaccatagc gatacgtcct tttgttttta gagcatgttg agcaatttga
     8761 caaatctctc taaggtttgt tgttatttca tgtcttgcaa ggagataatg cgggcttaga
     8821 ttttttttag aggtttcgct tgctttgaaa taaggtggat tgcaaagcat taaatcaacg
     8881 tttgaacgtt gaacatgatc aagaagattt tttaaatcat cattaatcat agtaacctgt
     8941 ttttccaatt tattgagctt aattgaacgc ttagccatat ccgcaaggcg ctcttgtagt
     9001 tcgatctcta taattgtcgc acttgttttg gtgctggcaa acaaaccgac agcaccatta
     9061 ccactgcata agtcaacgat aagaccacga gaaggaagtt tagggaaccg agacaataaa
     9121 acactatcta tggagtagga aaaaacttct ttgttttgga tgattttgac atcagttgaa
     9181 aaaagctgat caatgcgttc attttctttt aaaattgtat ctatcatagg cttattatag
     9241 caaaattaca gcttcctagc actagttttg aaagtgtgga ggttgatttt aagataattc
     9301 tttctaaaaa aaaggaagca tgataaaata gaagttactt gatgaaaggt agaggagaaa
     9361 catgttctat acttacttac gtactttagt tatgttcttg atttgggtgg ctaatggtaa
     9421 tgcgcactat cataatgaag ataaaatgtt aaaagatgat gaaaattata ttttagtagc
     9481 tccacacaga acattttggg atcctgtgta catggctttt gctgctagac ccaaacagtt
     9541 tgtttttatg gccaaaaaag aattattcac gaatcgtttg tttggttggt ggattaaaat
     9601 gtgcggtgct ttccctattg atcgtgaaaa gccagggcaa gacgctattc gatatcctgt
     9661 aaagatgctg aaaaatagta accgttcttt ggtgatgttt ccgagtggaa gccgccactc
     9721 caaagatgtc aaaggaggcg tagctgttat tgcaaaaatg gcaaaagtca gaattatgcc
     9781 agcagcatat agaggtccta tggtttttaa aaatctatta aaggggcatc gtgttgatat
     9841 gaattttggt aaccctattg atgtttctga tattaagcgt atggatgccg aagggattgc
     9901 agaagtatct cgtcgtattc aggaagaatt tgatcgttta gatagagaaa atgaaactta
     9961 tgatgatggt aagaaattaa atccattgac ttatatttat cgtctacctt tagcaattat
    10021 agctattgtc ttgcttgtcc taactctaat ttttagttat ttagcaagct ttgtgtggga
    10081 tccacaaaaa catctaaaat aagggttaca tggaaactat ttacttttta gtaaatagtt
    10141 ttttttgttt ttaataggaa aatctcttta gttacgaata agtaaatgga ggaatatttt
    10201 tatgtttgaa atagtat
//
LOCUS       DATRQA010000067        10165 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.067, whole genome shotgun sequence.
ACCESSION   DATRQA010000067 DATRQA010000000
VERSION     DATRQA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.067"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1368)
                     /locus_tag="VBD27_001247"
     CDS             complement(<1..1368)
                     /locus_tag="VBD27_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7025257.1"
                     /translation="MLFDFLEEKIKLKAYLMTALLDDGEINLDHFKTEYALSEKILQG
                     LISELQILYSDFYLVVKSRTMVLFHYETLSKLETLHTIYRESNTLQFLAYFIAPQNVS
                     FSEFTAKKFISVASAYRIKKNCKLYLESVKLSIQKNKVTGPEYQIRLLIALLQYHFGF
                     IIYPFDATSEVMIRDFIEYSNFKGCDYDLKDLSEEYYYFYILVNLVWKRKEYKVEIPQ
                     TESFNQLKKSILYMELSKAAQKSIAIHLGIELNEGLLDYLYLVFCISDGPLFSNRDDI
                     NINEEFIFASYKISKSKAFKNFCGRLLGREFIDSQHFKDINAYFLRHFICYCYYFIPD
                     FYFLNTSRLSYSKDLYHLLDKGLADIFNLKGGNLTFSKHETVLLTMQLSNLIETFLAP
                     LSVYVISSSNIRLQTYQVMLNQYFTSKIAEFFFVNYQTTQIDEKLLKKADIIIAERRY
                     ISSLKN"
     gene            complement(1500..1742)
                     /locus_tag="VBD27_001248"
     CDS             complement(1500..1742)
                     /locus_tag="VBD27_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEO7025258.1"
                     /translation="MDTIENLIIAIVKPLISQPDQLTIKIQDGPEFLEYHLDLDTQDI
                     GRVIGKKGRTITAIRSIVYSVPTQGKKVRLIIDEKE"
     gene            complement(1752..2024)
                     /gene="rpsP"
                     /locus_tag="VBD27_001249"
     CDS             complement(1752..2024)
                     /gene="rpsP"
                     /locus_tag="VBD27_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO7025259.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQVTIKEERVLEWLSKGAQPSDTVRNLLSKAGVMTKFHDQKFSK"
     gene            complement(2153..3400)
                     /locus_tag="VBD27_001250"
     CDS             complement(2153..3400)
                     /locus_tag="VBD27_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025260.1"
                     /translation="MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKD
                     SVTNEITKSQKNLQIYYKTKEDQKNEDNFGAQGAFMQGSDTNRKEPIIQESWLKKIAK
                     EVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGINRTYLGIKKFKIKSGRQFQEEDYNQ
                     FSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNA
                     ILTNTQLASEFGAKEAENIYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTS
                     IVKSINTQVGIMTGVIGAIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRRK
                     ILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSLIFSAFIG
                     IIFGLLPANKASKLNPIDALRYE"
     gene            complement(3415..4125)
                     /locus_tag="VBD27_001251"
     CDS             complement(3415..4125)
                     /locus_tag="VBD27_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025261.1"
                     /translation="MTTNRKELIRLHQIVKSYQNGDQKLQVLKNIDLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDSPTSGDYSLNGKRVEELSQTKLAQVRNKEIGFVFQQFFLL
                     SKLTALQNVELPLIYAGVAPKKRKNLAKQFLDKVELRERMNHLPTELSGGQKQRVAIA
                     RALVNSPSIILADEPTGALDTKTGEQIMQFLTELNQEGKTIIMVTHEPEIADYATRKI
                     VIRDGEITADTTDSIRID"
     gene            complement(4128..5372)
                     /locus_tag="VBD27_001252"
     CDS             complement(4128..5372)
                     /locus_tag="VBD27_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO7025262.1"
                     /translation="MSKRQNLGISKKGAIISGLSVALIVAVGGFLWVQSQPNKSAVKT
                     NYKVFNVREGSVSSSTLLTGKAKANQEQYVYFDANKGNRATVTVKVGDKITAGQQLVQ
                     YDTTTAQAAYDTANRQLNKVARQINNLKTTGSFPAMESSDQSSSSSQGQGTQSTSGAT
                     NRLQQNYQSQANASYNQQLQDLNDAYADAQAEVNKAQKALNDTVITSDVSGTVVEVNS
                     DIDPASKTSQVLVHVATEGKLQVQGTMSEYDLANVKKDQAVKIKSKVYPDKEWEGKIS
                     YISNYPEAEANNNDSNNGSSAVNYKYKVDITSPLDALKQGFTVSVEVVNGDKHLIVPT
                     SSVINKDNKHFVWVYNDSNRKISKVEVKIGKADAKTQEILSGLKAGQIVVTNPSKTFK
                     DGQKIDNIESIDLKSNKKSEVK"
     gene            complement(5574..7172)
                     /locus_tag="VBD27_001253"
     CDS             complement(5574..7172)
                     /locus_tag="VBD27_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEO7025263.1"
                     /translation="MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRNRPVSILI
                     SKELNHVIHHYDAQFENLETLVGYINTHEDSLIYPLCLDKNHELFQEFLAWQSHYSRQ
                     YISDNLSEKSVQAIDNNQDFVQVDCLVMRDSLNNCLYVSDLEYIESNKTTGKSLAIVP
                     SQTLSDAARQTIRDVAFDVCRKANIIGVCYFSFLIDLNSLDYHIISLSSGLSYQSILF
                     ETITTYPVLEIATKLTVGYTFSQLKHSYYPNTSAFLEPQLDYVATVSFSFEKVDYIFF
                     ARNIEQLFLNLLEASSHDHFPFLSDISEEDLMFALIQKKENRLAYLLEAFRRGFDLYD
                     LSSVTKINPFYLDKCLHIVELYENLNKSQYNVDIYKDAKRYGFSDDYIASSWQISLID
                     MLEYRKKHSVAPVLKQVEQSSGVLTGHQIQYFRSYDWHSDYSSSGCQKALIMVDKGYS
                     LVKLNELIKQIKQTHLELLIVTNQPLLIEQLNDTSIIFDTIGIETILTIMGIEEVEEV
                     YLLGESQADMFSKMKNFSISCQSI"
     gene            complement(7228..8298)
                     /locus_tag="VBD27_001254"
     CDS             complement(7228..8298)
                     /locus_tag="VBD27_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEO7025264.1"
                     /translation="MAKKLLILEDGTVFEGLSFGSSLDVTGELVFCTGNTGYQEIITN
                     PSHNGKILVFTSPLIGNYGIHRSYSEAIIPTCLGVVVAEYSRCVSSDTSKMNLDEFLK
                     MKKVPAMSGVDTRYLMQVIKEKGFVKATLAEAGDVLSHLQDQLIATVLPTNNVEQVST
                     KTAYPSPASGRNIVVLDFGLKHSVLRELSKRQCDVTVIPYNTSLEGIKNLYPEGIILS
                     NGPGNPEKLQEVLNTIKELQKSVPMLGIGLGHQLIAMANGAEIMRLPVAKKGPNYPMR
                     DIATGRLETVIQFNHFTVNRLNLPHDLLVTHEGLNDQEIVALRHRSFPVMSVQFYPEA
                     APGPHDVTYFFDEFLEMIGSRA"
     gene            complement(8347..8868)
                     /gene="pyrR"
                     /locus_tag="VBD27_001255"
     CDS             complement(8347..8868)
                     /gene="pyrR"
                     /locus_tag="VBD27_001255"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEO7025265.1"
                     /translation="MKRKEIIDDVTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     LAKRIQERLKQLENLDIPVGELDTKPFRDDMKVEVDTTTMPVDITDKDIILIDDVLYT
                     GRTIRAAIDNLVSLGRPSRVSLAVLIDRGHRELPIRADYVGKNIPTSQFEEILVEVME
                     HDGYDRVSIIDPS"
     gene            complement(9044..9934)
                     /locus_tag="VBD27_001256"
     CDS             complement(9044..9934)
                     /locus_tag="VBD27_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7025266.1"
                     /translation="MEITIKIAGVRLDKALADNSELSRSQANEEIKKGIVLVNGQVKK
                     AKYTVQEGDRITFDIPKEEVLDYQAENIPLDIIYQDDDVAVVNKPQGMVVHPSAGHSS
                     GTLVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKNDRAHQVLAEELKDKKS
                     LRKYLAIVHGNLPNDRGVIEAPIGRSDKDRKKQAVTAKGKPAITRFHVLERFGDYTLV
                     ELSLETGRTHQIRVHMAYIGHPLAGDPVYGPRKTLGGKGQFLHAQTLGFTHPSNGENL
                     IFSVEVPEIFQTTLDKLRKN"
     gene            complement(9918..>10165)
                     /locus_tag="VBD27_001257"
     CDS             complement(9918..>10165)
                     /locus_tag="VBD27_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO7025267.1"
                     /translation="AIIYLMKHINGSYWLLISLTLIISGGLGNFIDRLRLGYVVDMVH
                     LDFINFAIFNVADSYLTIGIICLMIALWKEESNGNHN"
BASE COUNT         3246 a         1959 c         1512 g         3448 t
ORIGIN      
        1 attttttaaa gaagatatat agcgtctctc agcaataatg atatctgctt tttttagtaa
       61 tttttcatct atctgagtcg tttgatagtt tacaaaaaag aattccgcta tcttagatgt
      121 aaaatattgg tttaacatta cttgataggt ttgtaatcta atattactag atgaaattac
      181 atatacagaa agaggagcta agaaagtctc tattaagtta gataattgca ttgttagtag
      241 cactgtctca tgttttgaaa aagttaaatt ccctccttta aggttaaaaa tatcagccag
      301 acctttatct aataggtgat ataaatcctt agaatagctc agccttgatg tatttaaaaa
      361 ataaaaatca ggaataaaat aataacaata acaaataaaa tgtctcaaaa aataggcatt
      421 aatatcttta aaatgctgcg agtcaataaa ttctctaccc aataatcgac cacaaaaatt
      481 cttaaaggct ttagactttg atatcttata agaagcaaaa ataaattctt cgttgatatt
      541 gatatcatct cgattgctaa atagaggacc atcactaata caaaacacta aatatagata
      601 atcaagtaaa ccttcattca actctatacc aagatgtata gcaatactct tttgagctgc
      661 tttggataac tccatgtaga gaatagattt ttttagttga ttaaaggatt ctgtttgagg
      721 aatttctact ttatattctt ttcttttcca aactaaattc accaaaatat aaaaatagta
      781 atattcttca gataagtctt taaggtcgta gtcacaccct ttaaaattcg agtattcaat
      841 aaaatccctt atcattacct cacttgtagc atcaaaagga taaataataa aaccaaaatg
      901 atactgtaac aaagcaatca gcagcctaat ctgatattct ggacctgtca ctttattttt
      961 ttgtattgat aacttaacac tctccaaata aagtttacag tttttcttta ttcggtaggc
     1021 acttgcgacg gatatgaatt tcttagccgt gaattcagaa aaactgacat tttgaggagc
     1081 aataaaatat gccaaaaact gtaacgtatt agactctcga taaatggtat gcaaagtctc
     1141 tagcttagaa agtgtttcgt aatggaacaa taccattgtt ctacttttga caacaaggta
     1201 gaaatcagaa taaagtattt gtagttccga aataagacct tgtaaaatct tttcactcaa
     1261 agcatattct gttttaaagt gatcaaggtt aatttctcca tcatcaagta aggcggtcat
     1321 caggtatgct ttcagtttta ttttttcttc gagaaaatca aatagcaata tcttatcctc
     1381 cttaataaaa aatcagaaaa ttccctttca catccattat attctaaaag actaaaaaac
     1441 tcacaatttt tgtgtagata taacaaaaaa gacaggaagt tcctatcttt tagaataaat
     1501 tattctttct catcaatgat gagtcttact tttttccctt gtgttggtac cgaatagaca
     1561 atcgacctta tcgctgtaat tgtacgaccc tttttgccga taacacgacc gatgtcttgg
     1621 gtatctaaat caagatggta ctctaaaaat tctgggccat cctgaatttt tatggttaac
     1681 tgatctggtt gtgaaatcaa aggtttcaca atagcaataa tgagattttc aatggtatcc
     1741 ataggctttt attattttga gaatttttga tcgtggaatt tagtcataac tccagctttt
     1801 gaaagaaggt tacgaactgt atctgaaggt tgtgcacctt ttgataacca ttcaagaaca
     1861 cgttcttctt tgattgtaac ttggttttct gcaactaatg ggttgtatgt tccaacagtt
     1921 tcaataaaac gtccgtcacg tggtgcacgt gaatctgcaa cattgatacg gtagaaaggt
     1981 ttcttcttag aacccatacg agttaaacgg atttttactg ccatttttaa atagtctctt
     2041 ttctttttct ttatatttgg tcgttgatag cttaactaac aactttacct tactaagtat
     2101 aacataaata caaactctgt caagaaaaaa tcttgacaga gtacatgttt ttttattcat
     2161 atcgtaaagc atcaattggg tttaatttgc tagctttatt agcaggtaat aaaccaaata
     2221 taattccaat aaatgctgag aaaattaaac tgcctatagc cacgttaagt gacacagaag
     2281 gagtaatctt atcttgcgca ttagcaatta acatcgtacc tccataggct agtaataacc
     2341 caattaaccc acccaaaatc gttaagacca ttgattcaat taaaaattga gctaaaattt
     2401 tacgtcgagt tgccccaagg gctttacgaa ggccaatttc tctcgtgcgc tcagttacag
     2461 agactaacat aatattcatc actccgatac caccaactag gagagaaatt gctgcaatcg
     2521 ctccaataac acctgtcata ataccaactt gggtattgat agatttaact atagatgtca
     2581 tatcaaaatt atcatagtac ccatccttag catggctaat gtctgttaaa cgtttgccta
     2641 tttctttacc aatgcgattg gattgcgaca catcattaag atgaaaatat atgttttctg
     2701 cttctttagc accaaactcc gaagctaact gtgtattggt taaaatagca ttcccatcag
     2761 aattacttcc atataaacct gagccagctt ctggatccga ataaaccccc actactaagt
     2821 aagatttatt ttttacagtc accactttat ttaacgctgc ttcatttgtc tgaaataatc
     2881 tctgagctaa tttttcttct agtaaaataa ctctcgagaa ttggttataa tcctcctctt
     2941 ggaattgacg accactttta attttgaact ttttgatacc aaggtaagtg cggttaattc
     3001 ctgttatatt aaccgtcttt gccttttttt caaggtaagc tactggagca tttgtttgat
     3061 tggttacata gtagccacta acaccatcta cttccttagc aatctttttt aaccatgatt
     3121 cttgaataat tggttcctta cggttggtat cactaccttg catgaaagcc ccttgagctc
     3181 caaagttatc ctcatttttt tggtcttctt ttgttttata ataaatttga agattcttct
     3241 gagactttgt aatctcattt gtaaccgaat ctttcatgcc cttacctaag gccataatta
     3301 acacaactga tgcaacccca atgataatcc ctaacatggt cagaaaagca cgcatcttgt
     3361 gccctagaat cgatgatagg gcaaatttcc aattttccat aatcgaatct ccccttaatc
     3421 aattctaata ctatcagtag tatctgctgt aatttcgcca tcacgaataa caattttacg
     3481 tgtcgcataa tcggcgattt ctggttcatg agttaccatg ataatggttt taccttcttg
     3541 atttaattcc gttaggaact gcataatttg ctcacccgtc tttgtatcca aggcaccagt
     3601 cggctcatct gctagaataa tagatgggct attcaccaaa gcacgcgcga ttgctacacg
     3661 ttgcttctgt cccccagata attctgttgg cagatggttc atacgctctc tcaattcaac
     3721 tttatctaaa aattgttttg ctaaattttt acgcttttta ggagctacac ccgcataaat
     3781 taagggtaat tcaacatttt gcaaagccgt tagtttagat aacaagaaaa attgttgaaa
     3841 gacaaaaccg atttccttat ttcgaacttg agctaatttt gtttgactta gttcttcgac
     3901 acgtttacca tttaaactat aatctcctga agttggggaa tccaacaagc caataatatt
     3961 cattagtgtg gatttccctg agccagatgg ccccatgatt gcaagaaatt caccttcata
     4021 aaccgtcaaa tcaatgttct ttaagacttg aagtttttga tccccatttt gataactttt
     4081 aacaatctga tgcaatctaa tcagttcttt tcgattagtt gtcacgatta tttcacctct
     4141 gatttcttat tagacttaag atcgattgat tcaatattat caattttttg cccatccttg
     4201 aaagttttgc ttggattagt aaccacgatt tgtcctgctt tcaaacctga taaaatttct
     4261 tgtgtcttag catcagcttt accaattttg acttcaactt tggaaatttt acgattagaa
     4321 tcattgtata cccaaacaaa gtgtttatta tctttgttta tcacagaact tgtagggaca
     4381 ataaggtgct tatctccatt aactacttca actgatacgg taaaaccttg ttttaatgca
     4441 tcgagagggc tagtaatatc tactttatat ttataattta cagcactaga gccgttatta
     4501 gagtcattgt tgtttgcttc tgcttctgga taatttgaga tatatgaaat tttaccttcc
     4561 cattccttgt caggatagac cttagatttt attttaacag cctgatcttt tttaacatta
     4621 gccaaatcat actcactcat cgttccttgt acttggagtt taccttcagt tgctacatgg
     4681 acaagtactt gactagtttt tgaagctgga tcaatatcac tattaacttc aacaactgtc
     4741 cctgatacgt cacttgtaat aacagtatca ttcaatgctt tttgtgcttt atttacttct
     4801 gcctgtgcat ctgcataagc atcattcaaa tcttgaagtt gttggttgta tgaagcatta
     4861 gcttgacttt gataattttg ctgtagacga ttcgtcgcac cactagtcga ttgagtccct
     4921 tgtccttgtg atgatgatga agattgatca cttgattcca tagctggaaa actccctgtt
     4981 gtctttagat tattaatctg acgcgctact ttatttaatt gacgattagc agtgtcgtag
     5041 gctgcttgtg cagttgttgt atcatattga actaactgct gaccagctgt gattttatca
     5101 cccactttaa ctgtaacagt tgctcgatta cctttattag catcaaaata cacatactgt
     5161 tcttgattag ccttagcttt tcctgtcaaa agagttgagg acgaaacact tccttctcta
     5221 acattaaaaa ctttgtaatt agtttttact gcactcttat taggttgaga ttgtacccat
     5281 aaaaagccac ctactgctac aattagtgcc actgagagcc ctgatataat tgctcctttt
     5341 ttactaatcc ctaaattttg tcgtttactc atgtccatct cctgtttgta ttattaatat
     5401 agaacaagtg tattattttt aaataatatt gtcaagaaaa ttctttaaaa ataatatttt
     5461 tactcattaa gtatactatt ttgctcatag aaagaactta caaaattgaa agtactttac
     5521 tttttaccac aaataaaagc tgagatctat atctcagctt ttattaagaa ttgctaaata
     5581 gactgacaag agatagaaaa atttttcatt tttgaaaaca tgtctgcttg actttctccc
     5641 aataaataca cttcttccac ctcttctata cccataattg ttaatatggt ctcaatacct
     5701 atagtgtcaa aaattataga tgtatcattt aattgttcta tgaggagagg ttgattggta
     5761 acaattaata attctaaatg agtttgtttg atttgcttaa ttaactcatt taattttact
     5821 agggaataac ccttgtctac cattatcaaa gccttttgac aaccgctaga actgtagtct
     5881 gaatgccaat catagcttcg aaaatactga atttgatggc cagttagaac accagaactt
     5941 tgttcgactt gcttcagaac aggtgcgaca ctatgttttt ttcgatattc taacatgtct
     6001 atcaaagata tttgccaact actagcaata tagtcatccg aaaaaccata ccttttagca
     6061 tccttgtaaa tatctacatt atattggctt ttattcaggt tttcatacaa ctctacaata
     6121 tgtaaacatt tatctaagta aaaagggttg attttagtaa ctgaggataa atcatacaaa
     6181 tcaaagccac gtctaaaagc ctctaaaaga taagctagac gattttcttt tttctgaatc
     6241 aaagcaaaca tcaaatcttc ctcgctaata tcactcagaa atggaaaatg atcatgactg
     6301 ctagcctcga gtaaatttaa aaataattgc tctatattac gagcaaaaaa gatatagtca
     6361 actttttcaa aactaaaaga tacggtagct acatagtcta attgcggctc taaaaaggca
     6421 gaggtatttg ggtaataaga atgcttcagt tgactaaaag tatatcccac tgtcaattta
     6481 gttgctatct ctaaaacagg ataggtcgta atagtctcaa ataaaatgct ttgataagac
     6541 aaaccagatg ataagctaat tatatgataa tccaaactgt tcaaatctat taagaaacta
     6601 aagtaacata ctccaataat gtttgccttt cgacacacat caaatgcaac atctcgaatt
     6661 gtttgcctag cagcatcgga taaagtttga cttggtacaa tcgctagaga cttaccagtt
     6721 gttttattag attcaatata ttctaaatca gagacatata aacaattatt caaagagtct
     6781 ctcattacta aacaatctac ttgcacaaag tcttgattat tgtctattgc ctgaacactt
     6841 ttttctgata aattatcact gatatactgc cttgaataat gactctgcca cgctaaaaac
     6901 tcttgaaata gctcatgatt tttatctaag caaaggggat aaatcaaact atcttcatgt
     6961 gtatttatat accccacaag cgtctcaaga ttttcaaatt gtgcatcata atggtgaatc
     7021 acatggttta gctccttcga gataagaata ctaacaggtc tattcctttt agataacctc
     7081 tcaacagttg atctaattaa atcgtcatat tctttcattt tctgtccgaa aataataagc
     7141 aaaataggct gtttatcctt tgtatttagc atatacgcac ctccttatta atcagctcac
     7201 cgactgactt taaataatac cttatgttta agcgcgtgat ccaatcattt ctaaaaattc
     7261 atcaaaaaaa taggtcacat catgagggcc tggagcagcc tctgggtaaa attgcacgga
     7321 catcactgga aatgagcggt gccttagagc aacaatctct tgatcattca aaccttcgtg
     7381 tgttaccagt aaatcatgcg gaagatttag ccggttaacg gtaaaatgat taaactgaat
     7441 aactgtttct aaacgccccg tagcaatatc tctcatggga taatttggcc ccttcttagc
     7501 aactgggagt ctcataattt ctgcaccatt tgccatggca attaattgat gccccaaacc
     7561 aatacctaac ataggaacag atttttgtaa ttctttaata gtgtttaaga cttcctgaag
     7621 cttttctgga tttcccggac cattagataa tatgatacct tctgggtata gatttttgat
     7681 accttctaaa gacgtattgt agggaatgac agtcacgtca cactgacgtt tagacaattc
     7741 tctcaaaaca gaatgtttaa ggccaaaatc aagtacaaca atatttcgac cgctagcagg
     7801 cgacggataa gctgtttttg tcgaaacttg ttccacatta tttgtcggta atactgtcgc
     7861 tattaattga tcttgtaaat gtgataaaac atcacctgct tctgctaaag tagcttttac
     7921 aaatcctttt tctttaataa cttgcatcaa ataccttgta tcgacccccg acattgcagg
     7981 aacctttttc attttcaaaa attcatctag attcatcttg ctagtatcgc tacttacaca
     8041 tctggaatac tctgcaacaa caactcctaa acaagtggga ataatggctt cagagtaact
     8101 acgatgaata ccataatttc ctattagcgg tgaggtaaaa actaaaattt ttccattatg
     8161 tgaaggattc gttattattt cctgatagcc ggtattcccc gtacaaaaaa ctaattcgcc
     8221 agttacatca agagatgaac caaaggatag tccttcaaaa accgtaccat cctctagaat
     8281 taacaatttt tttgccattg tggttcttcc ccttctataa aatcatcgta aataatagtc
     8341 gtctacttaa ctcggatcaa taatactcac acgatcgtaa ccatcatgtt ccatcacttc
     8401 aactagaatt tcttcaaatt gacttgttgg aatatttttc cccacataat ctgcacgaat
     8461 gggtagttca cgatgcccac ggtcaattaa tacagctaaa ctgacacgac tcggacgccc
     8521 caaactaaca agattatcaa tagcagctcg aatagtgcga cctgtataga gaacatcatc
     8581 aattaatata atatctttat cagtgatgtc aactggcata gttgtggtgt caacttcaac
     8641 tttcatatca tcacggaaag gtttagtatc caattctcca acgggaatat ctaaattttc
     8701 taattgcttc aaacgttctt gaatgcgttt ggctaaaaaa acacctcttg ttttaatgcc
     8761 tgctaaaaca atattatcta aatttttgtt acgttctata atttcgtagg taatacgtgt
     8821 aatagcacgt ttcatggtca catcatcaat aatttctttt cttttcattg cagtatcctc
     8881 tctaagataa aagagcccct tcaaaaggag actctctaga atgacaaagc cgctcaggca
     8941 ttgatttagt ctgaagtttc tccttaacta tctcactgga taaggttaaa ggaatgattt
     9001 gcttatatta tagcaaatgg taaaagtatt tttcaagaaa aacttaattt tttctcaact
     9061 tgtctaaagt tgtctgaaaa atttccggaa cctctactga aaaaatcaaa ttctctccgt
     9121 tactagggtg cgtaaaacct agtgtttgtg catgcaaaaa ctgtccttta ccccctaaag
     9181 tcttgcgggg accgtagaca ggatcaccgg ctagaggatg gccaatataa gccatatgaa
     9241 cccgaatttg gtgcgttcga cctgtctcca atgacaattc aactaaggta taatcaccaa
     9301 aacgttctaa aacatgaaaa cgagttattg ctggcttacc tttagcagtc acggcttgct
     9361 tcttacgatc tttatcactt ctaccaatcg gagcctcaat cacgcctcga tcatttggta
     9421 aattaccatg cacaattgct aaatatttac gaagcgattt tttatctttc aactcttcag
     9481 ccaacacttg gtgagctcta tcattttttg caaccatcaa taaaccagaa gtatctttat
     9541 caatacgatg aacgatccct gggcgaacaa cgccattaat tgatgataaa tccttaatat
     9601 gatacatcaa ggcgttaact aaggtgcccg aactgtggcc tgcagaaggg tgaacaacca
     9661 tcccttgcgg cttattaacc acagcaacgt catcatcttg atagataata tctaaaggga
     9721 tattttcagc ttgataatct agtacttcct ctttaggaat atcaaaagta atccgatctc
     9781 cttcttgaac agtatatttc gcttttttta cttgtccatt tactaacaca atcccttttt
     9841 taatttcttc attagcttga ctacgcgaca gttcagaatt atcagctaaa gctttatcta
     9901 gcctaactcc tgctatttta attgtgattt ccattacttt cctctttcca aagtgcaatc
     9961 attaagcaaa taataccgat tgtcagatat gagtctgcta cattaaagat agcaaaatta
    10021 ataaaatcta aatgcaccat atcaacgaca taacctaatc ttaagcgatc tataaagttt
    10081 cctaatccac cagagatgat taaggttaga gaaattaata accaataact tccattaata
    10141 tgtttcatca aataaataat agcaa
//
LOCUS       DATRQA010000068         9843 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.068, whole genome shotgun sequence.
ACCESSION   DATRQA010000068 DATRQA010000000
VERSION     DATRQA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9843
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.068"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..381
                     /locus_tag="VBD27_001258"
     CDS             <1..381
                     /locus_tag="VBD27_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7025268.1"
                     /translation="YESRKLQSKNYYKLTLLTLTPLIFDQNLPFLVGLPVILQPILFI
                     PMVLTTIFAEAFGALMLYLKFVDPAVYTVPSGTPSLLFGFLASNGDWRYLPVTAIILV
                     VGFFIYRPFVKIAFAKEEQYEKIT"
     gene            365..1207
                     /locus_tag="VBD27_001259"
     CDS             365..1207
                     /locus_tag="VBD27_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7025269.1"
                     /translation="MKKLLRWLPPVLFIIILIGMTILGKSYINKVTAHKIKLYNSRMT
                     PTILIPGSSATQERFNSMLAQLNQMGEKHSVLKLTVKKDNSIIYNGQISGNDHKPYIV
                     IGFENNEDGYSNIKKQTKWLQIAMNDLQKKYKFKRFNAIGHSNGGLSWTIFLEDYYDS
                     DEFDMKSLLTMGTPFNFEESNTSNHTQMLKDLISNKGNIPSSLMVYNLAGTNSYDGDK
                     IVPFASVETGKYIFQETAKHYTQLTVTGNNATHSDLPDNPEVIQYVAEKILKNEKGKL
                     PKPH"
     gene            1284..2111
                     /locus_tag="VBD27_001260"
     CDS             1284..2111
                     /locus_tag="VBD27_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO7025270.1"
                     /translation="MKLISWNIDSLNAALTSESTRALMSRQVIDTLVAEDADIIAIQE
                     TKLSAKGPTKKHLEVLETYFPEYDLVWRSSVEPARKGYAGTMFLYRKGLNPIVSFPEI
                     DAPTTMDNEGRIITLELENCYITQVYTPNAGDGLKRLADRQIWDIKYAEYLATLDSQK
                     PVLATGDYNVAHKEIDLANPSSNRRSAGFTAEERQGFTNLLAKGFTDTFRYLHGDVPN
                     VYSWWAQRSRTSKINNTGWRIDYWLTSNRVADKITKSEMIHSGDRQDHTPIILEIEL"
     gene            complement(2151..2507)
                     /locus_tag="VBD27_001261"
     CDS             complement(2151..2507)
                     /locus_tag="VBD27_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEO7025271.1"
                     /translation="MYTFYEYPKCTTCRSAKKELTELGLTFEAIDIKSNPPKASLLKE
                     LLENSPYDLKKFFNTSGNSYRELGLKDKFDDLTLDQALDLLASDGMLIKRPLLVKDNK
                     ILQIGYRTKYKDLNLV"
     gene            complement(2509..2985)
                     /locus_tag="VBD27_001262"
     CDS             complement(2509..2985)
                     /locus_tag="VBD27_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO7025272.1"
                     /translation="MLYQEFYQSPLGEIRLLADNLGLSGLYFVGQKYDMLAVNQEEIV
                     NMSNSYTLLGKKWLDAYFSQQNLPSIPLSLRGTAFQTRVWQELQKIPFGDTKTYGELA
                     KELNCQSAQAVGGAIGKNPISLIIPCHRVLGRYGQLTGYAGGLERKSWLLEYEKEK"
     gene            complement(3040..4190)
                     /locus_tag="VBD27_001263"
                     /pseudo
     CDS             complement(3040..4190)
                     /locus_tag="VBD27_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            complement(4263..4811)
                     /locus_tag="VBD27_001264"
     CDS             complement(4263..4811)
                     /locus_tag="VBD27_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO7025273.1"
                     /translation="MQIRLAFPNEIDQIMLLIEEARAEIAKTGSDQWQKEDGYPNRND
                     IIDDILNGYAWVGIEDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISN
                     QFRGRGLAQTFLQGLIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPLDGVRLA
                     YQKIKEKGETSIYREIDERNPM"
     gene            complement(4879..5970)
                     /gene="serC"
                     /locus_tag="VBD27_001265"
     CDS             complement(4879..5970)
                     /gene="serC"
                     /locus_tag="VBD27_001265"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEO7025274.1"
                     /translation="MIIYNFSAGPAVLPKPVLVKAQSELLNYQGSSMSVLEVSHRSKE
                     FDDIIKGAERYLRDLMGIPDNYKVIFLQGGASLQFSMIPLNITRGRKAYYHVAGSWGK
                     KAYTEAVKLSKTIPFEPILLASSEESVYDYIPEFDEKEIDPKAAYVHVTTNNTIEGTS
                     LYDIPKTNGVPVIADMSSNILAVKYKVEDFAMIYAGAQKNIGPAGVTVVIIREDMINE
                     EPTLSSMLDYKVQSDAGSLYNTPPAYSIYIAKLVFEWVKSLGGVDAMEKANREKSGLL
                     YDYIDSSEFYSNPVRDKKSRSLCNIPFITINKDLDEKFVKEATERGFKNIKGHRSVGG
                     MRASLYNAFPKQGVIELIDFMKTFEAENT"
     gene            6103..6738
                     /locus_tag="VBD27_001266"
     CDS             6103..6738
                     /locus_tag="VBD27_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEO7025275.1"
                     /translation="MILREFCAENLTDLTRLDKAIISRVELCDNLAVGGTTPSYGVIK
                     EANQYLHEKGISVAVMIRPRGGNFVYNDLELRIMEEDILRAVELESDALVLGILTSNN
                     HIDTEAIEQLLPATQGLPLVFHMAFDVIPKSDQKKSIDQLVALGFTRILLHGSSNGEP
                     IIENIKHIKALVEYANNRIEIMVGGGVTAENYQYICQETGIKQAHGTRITQ"
     gene            complement(7008..7877)
                     /gene="rsmI"
                     /locus_tag="VBD27_001267"
     CDS             complement(7008..7877)
                     /gene="rsmI"
                     /locus_tag="VBD27_001267"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO7025276.1"
                     /translation="MEMQVQKSFKSNIHYGTLYLVPTPIGNLDDMTFRAIRILREVDF
                     ICAEDTRNTGLLLKHFDITTKQISFHEHNAYDKISGLIDLLKEGKSLAQVSDAGMPSI
                     SDPGHDLVKAAIEGDIPVVSIPGASAGITALIASGLAPQPHIFYGFLPRKKGQQITFF
                     ETKQDYPETQIFYESPFRVSDTLKHMKEIYGDRQVVLVRELTKLYEEYQRGTISQLLE
                     HIEKVPLKGECLIIVDGKRDTERVKDSSQQDPLVLVKEYIANGDKTNQAIKKVAKEFN
                     LNRQELYASFHDL"
     gene            complement(7877..8203)
                     /gene="yabA"
                     /locus_tag="VBD27_001268"
     CDS             complement(7877..8203)
                     /gene="yabA"
                     /locus_tag="VBD27_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEO7025277.1"
                     /translation="MDKKDLFDAFDDFSQNLLVGLSEIETMKKQIQKLLEENTVLRIE
                     NGKLRERLSVIEAETETAVKNSKQGRELLEGIYNDGFHICNTFYGQRRENDEECAFCI
                     ELLYRD"
     gene            complement(8233..9096)
                     /locus_tag="VBD27_001269"
     CDS             complement(8233..9096)
                     /locus_tag="VBD27_001269"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO7025278.1"
                     /translation="MDLKRTQPKLLEKFNTILQSDRMSHAYLFSGNFASLDMALYLAQ
                     SQFCEKRQSGLPCQECRACRLIANGEFSDVKIIEPQGQLIKIETIKELTKDFSRSGFE
                     GKSQVFIIKYCEKMHVNAANSLLKFIEEPQSSSYVILLTNDENNVLPTIKSRTQIFRF
                     PKQLDMLVHQAEQAGLLKSQASLLAQVADDPKHLEILLTNKKLLDYLNLSQQFVTTLA
                     KDRQTAYLEVSRLTSQVVDKNDQAFVFQWLTIMLAKEGQLYDLENTYRAQQMWKSNVS
                     FQNSLEYMVLS"
     gene            complement(9116..9751)
                     /locus_tag="VBD27_001270"
     CDS             complement(9116..9751)
                     /locus_tag="VBD27_001270"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO7025279.1"
                     /translation="MKKGLMISFEGPDGAGKTTVLEAVLPLLREKLSQDILTTREPGG
                     VTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDS
                     SVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREVNRLD
                     LEQLDMHQRVRQGYLELADSEPNRIVTIDASQQLDEVIAETFSIILDRINQ"
BASE COUNT         3114 a         1867 c         1569 g         3293 t
ORIGIN      
        1 tatgaaagta gaaaactaca atcgaagaat tactataaat tgacattact gaccttaact
       61 cctcttattt ttgatcaaaa tttaccattt ttagtaggtc tacctgtcat tttacaacca
      121 atattattca ttccgatggt attaacaact atctttgcag aggcttttgg agctcttatg
      181 ctctacctaa aatttgtcga ccctgcagtt tatactgtcc caagtggtac acctagtcta
      241 ctatttggct ttcttgcttc aaatggggat tggcgttacc ttccagtcac tgcaattatt
      301 ctagttgttg gcttctttat ttatcgtccg tttgtcaaaa tcgctttcgc taaggaggag
      361 caatatgaaa aaattactta gatggcttcc tcctgtactt ttcattatta tccttatagg
      421 aatgactatc ttaggtaagt cctatatcaa taaagtaaca gctcacaaaa taaaactcta
      481 taactctcga atgactccta ctattttaat tccaggatcc agtgctactc aagaacgatt
      541 taacagcatg ttagcacagc tcaaccaaat gggagaaaaa catagcgttt taaagttaac
      601 tgtcaaaaaa gacaatagca ttatctacaa tggacaaatt agcggcaatg accacaaacc
      661 ctacattgtc attggatttg aaaataatga agatggttat agtaacatca aaaaacaaac
      721 aaaatggcta cagattgcta tgaatgatct tcagaagaaa tataaattta aacgttttaa
      781 cgctatcggt cattcaaatg gtggcttatc atggactatt ttcctagaag attattacga
      841 ctctgatgaa tttgatatga aatcattgtt aacaatggga acacctttta actttgaaga
      901 aagtaacacc tcaaatcata ctcaaatgct taaagattta atcagtaata aaggaaatat
      961 tccatcaagt ctcatggtat acaatttggc aggaactaat tcatatgatg gtgataaaat
     1021 tgttccattt gctagtgtgg agactggtaa atatattttc caagaaaccg ctaaacacta
     1081 tacccaacta acagtaactg gtaataatgc tacacattct gacttgcctg ataatcctga
     1141 agttatccaa tatgtcgcag aaaaaattct taaaaatgag aaaggtaaat taccaaaacc
     1201 tcactaaagc tgtagaatag caacgcctct ggtattttga tataatatat tgcgaattaa
     1261 actaaataaa aaaggatatt attatgaaac tcatttcatg gaatattgat tccctcaatg
     1321 cggcattaac aagtgaatca acacgtgctt taatgtcacg ccaagttatt gatactttag
     1381 ttgctgaaga tgccgacatc attgcgattc aagaaactaa actgtctgcc aaaggaccta
     1441 ctaaaaaaca tttggaagta cttgaaactt acttcccaga atatgatttg gtttggcgat
     1501 catcagtcga acctgctcgt aagggctatg ctggtacaat gttcttatat cgtaaagggt
     1561 taaatcctat tgttagcttc cctgaaatcg atgctcctac tacaatggat aatgaaggtc
     1621 gcattattac tctcgaattg gaaaactgct atatcacaca ggtctatact ccaaatgcag
     1681 gtgatggcct aaagcgctta gctgaccgtc aaatatggga tattaaatat gctgagtatt
     1741 tagcaacatt ggatagtcaa aaacctgttc tagcaacagg tgactataac gtagcccaca
     1801 aggaaataga cctagctaat cctagcagta atcgtcgttc tgctggattc acagctgaag
     1861 agcgccaagg ctttacaaat ctactagcca aaggttttac agatactttt cgttaccttc
     1921 atggcgatgt tccaaatgtt tatagctggt gggcacaacg aagtcgtaca tccaaaatca
     1981 acaatacagg gtggagaatt gattattggc taacaagtaa ccgtgtcgca gataaaatta
     2041 ccaagtcaga aatgattcat tctggtgatc gtcaagatca tacaccaatt attttggaga
     2101 ttgaactata aaccaaaaaa agatgattga gttataactc aatcatcttt ttatactaag
     2161 ttgaggtctt tatacttagt acggtagcca atctgaagaa ttttattatc tttaactagc
     2221 aatggacgct taataagcat tccatccgat gccaataagt ccaaagcttg gtcaagagtt
     2281 aaatcatcaa atttatcttt taagcctaat tctctgtaag aatttcctga agtattgaaa
     2341 aactttttaa ggtcataagg cgaattctct aacaattctt ttaataacga agctttggga
     2401 ggattgcttt ttatatcaat cgcttcaaaa gtcaagccga gttcagttaa ctcttttttt
     2461 gctgaacgac aagtggtaca tttggggtat tcataaaacg tatacatatt atttttcctt
     2521 ttcgtattct aataaccaag attttctttc taagccacca gcataaccag ttaattgacc
     2581 atatcttcct aaaacacggt gacaaggtat aattagagaa ataggatttt tacctattgc
     2641 tcctccaaca gcctgtgcag attgacaatt tagctctttc gctaattctc cataagtttt
     2701 ggtatctcca aaagggattt tctgtaattc ctgccatact ctcgtctgaa atgctgtccc
     2761 tctcaatgat aatggaatac taggtaaatt ttgctgtgaa aaataagcat ctaaccactt
     2821 cttaccaagt aaagtatagg aatttgacat attaacaatt tcctcttggt tgactgctag
     2881 catatcatat ttttgcccaa caaaataaag ccctgataaa cctagattgt ctgctagaag
     2941 gcgaatttct ccaagagggg actgatagaa ttcttgatat aacatatgat aagcataact
     3001 taaaaagcca tgaaaagcaa ttcatgactt tttagttctt tattgttgtt ttgcaattaa
     3061 acgtacacgg acgatattct catcaccttc aaattcttca attaaagtag aaatcttgtt
     3121 attatccgtt tcatctaaat ctattagtgt gtaggcgtag tcaccctttg aacggttaat
     3181 aatgttatca atatttatac ctaacactga tacagcagtt gaaattttag ccacaatgtt
     3241 aggaacattc ttgttaatta aagtaatacg aaatggtgcg gtttgaattt gatgtacatt
     3301 ggggaaatta actgaattag tgatttctcc tgtctccata aaacaacgaa tggtttgact
     3361 tgccataata gcacaattta gctctgcctc atctgttgat ccgcctacat gaggaaagac
     3421 tgtaattcct ttttggttta ataattcttt gtctccaaaa tcagtaatat agcgcttgac
     3481 aacaccagtt tctatcgctt caaatagctc ttggttattg actaattctg cacgagcaaa
     3541 gttgataatc gtagttcctt ttttcatgat tgaaaaagct tccgcatcaa aagtatgctt
     3601 agtttcattt gttaagggaa catgaattgt gatatagtca caagtttcaa aaatatcctt
     3661 aatctcttta accctttgaa catggcttga aatattccaa gctgtttcaa ttgaaatata
     3721 gggatcataa ccaagaactg tcattcctaa gcgtctagca cattcgcaat tctagctcca
     3781 atggcaccaa ggccgataac tcccaatttt tttcctgcaa tttcattacc agcaaaagct
     3841 ttctttcctg cttcaatttg tttgggaata tctgttccag ttagagtatt aacccatcgg
     3901 ttagctccta aataatcacg agctgaaagt aataaggcag caatgaccgc ttcttttaca
     3961 gcattagcat ttgcacctgg ggtattaaac acgactattc cctgtgcact tgcctcttca
     4021 ataggaatat tatttgttcc tgcacccgcc ctagcaatag ctttgaggtt acttgggaaa
     4081 tcctgattat gcaaattttg actacgaata atataggcat caggattttc tgacatatcc
     4141 ccatcaatct ggaaacgatt ccctaactct tgtaaaccaa tttgattaat attattaaat
     4201 gttttgacac taaggttatc tctttagtca tcattgacca aaataacccc tttctttttt
     4261 tcttacatgg gatttctttc atcaatctca cgatagatgc tcgtttctcc cttttctttt
     4321 atcttctgat aagccaatcg taccccatct agaggtactt taccacaata ttgatagcct
     4381 aatttattta gaatatgctg catagtgaca tttttttcat gagtatcgca acgaaaatca
     4441 ggtcctttat gaccttcaat taacccttga aggaatgtct gtgctagtcc tctaccacga
     4501 aattgatttg agatcgcaat acgatgaaat gtaagatagc gatggttatc atggagccat
     4561 tttccctcat aaatagcatc ataaacctct tcatgaccat caatgacagc cgcataagtc
     4621 gctagcatcc catcttcaat accaacccaa gcataaccgt tcaagatatc atcaataata
     4681 tcgttacgat tagggtatcc atcttctttt tgccattgat cacttcctgt tttagcaatt
     4741 tcggcacgcg cttcctcaat aagaagcata atttggtcaa tttcatttgg aaaagctaat
     4801 ctgatttgca ttatattacc cacttttata tattatagac tataataacg gtttaatttt
     4861 ctttcgtgat aacatttact atgtattttc tgcttcaaat gttttcataa aatcaattaa
     4921 ttcgatgaca ccttgtttag gaaaggcatt ataaaggcta gctctcatac cacctaccga
     4981 acgatgtcct ttaatatttt taaatccacg ttctgtcgct tcctttacaa atttttcatc
     5041 taaatcttta ttaatagtta tgaaaggaat gttacataga gaacgactct ttttatctct
     5101 gacaggatta ctataaaatt ctgaggaatc aatataatca tagagaagtc ctgatttttc
     5161 acggttagct ttttccatag catcaacacc acctagactt ttcacccatt caaagactaa
     5221 tttagcaatg taaatactgt aagcaggcgg cgtattataa agagaaccag catctgactg
     5281 aaccttataa tccaacattg atgataaagt tggttcctca ttaatcatat cttcacggat
     5341 aataactacc gttacaccag caggtccaat atttttttga gctcctgcat aaatcattgc
     5401 aaaatcttct actttatatt ttacagctaa tatattagaa gacatatccg caataacagg
     5461 cactccatta gttttaggaa tatcataaag cgatgttcct tcaatggtat tatttgttgt
     5521 cacatgaaca taggcagctt taggatcaat ctctttttca tcaaactctg gaatataatc
     5581 ataaactgat tcttctgatg aagctaatag aattggttca aaaggaattg tttttgatag
     5641 ttttacagcc tctgtataag cttttttccc ccacgaacca gcaacatgat agtaagcttt
     5701 acgccctctg gtaatattca agggaatcat actaaactgt aaggatgcac caccttgtaa
     5761 aaagattact ttataattat ccggaattcc catcaaatct cttaagtaac gctcagcacc
     5821 tttgataata tcatcaaact ctttggagcg gtgcgatacc tccaaaacac tcatactaga
     5881 cccttgataa ttaaggagtt cagattgggc ttttacaagc actggttttg gtaagactgc
     5941 aggacctgca gaaaaattat aaattatcat ataacacctc catataattg tgtaagatta
     6001 tagcacagta ttttgaatat tgtaaagatt tttttgatta attttgcttt ttattcgtga
     6061 tataatggta aaggcgaaca atcaagttta aaggagtaat ttatgatttt gagggaattt
     6121 tgtgcagaga atttaacaga cttaaccaga ttagataaag ctattatttc acgtgtcgag
     6181 ctttgtgata accttgctgt cggaggcact actcctagct acggtgttat caaagaagct
     6241 aatcaatatt tacatgaaaa aggtattagt gtcgctgtta tgattcgtcc ccgtggagga
     6301 aattttgtat acaatgattt agaattacgt attatggaag aggatatcct acgtgctgtt
     6361 gagttagaat cagatgctct tgttttggga atattaactt caaataatca tattgatact
     6421 gaagccattg aacaacttct acctgctacc caaggtttgc cacttgtttt tcatatggca
     6481 tttgatgtta tcccgaaatc tgaccaaaaa aaatcaatag atcaattagt tgctttaggc
     6541 tttacaagga ttctcctaca tggttcatct aatggcgaac caattataga aaatatcaaa
     6601 catatcaaag cattagttga atatgctaat aatcgtattg aaattatggt tggcggcggt
     6661 gtgactgctg aaaactatca atatatttgc caagaaactg ggattaaaca agcccatggt
     6721 actagaataa ctcaataaaa aattcgaaac agggctataa gtttctttag tatatttact
     6781 actaagttct ctcatgtttt gtaaaacctg aaagaaccgg aataagagca taccatcttt
     6841 tataacctac tctcagaaat aaaatccaat ttgcaatggt attcaaaaga gctgaaatta
     6901 aaaattttaa ggaaattgga agtatcttac ttctttattc caatagctaa atcggagatt
     6961 ataaaaggtt gccaaatttt tctttggcaa cctttttata ttattactta taaatcatgg
     7021 aaactagcat agagttcttg tctattgaga ttaaattctt ttgctacttt ttttatcgct
     7081 tgattagttt tatcaccatt agcgatatat tcttttacta atactagtgg atcttgttgg
     7141 ctactgtctt tcactcgctc ggtatctctc ttaccatcaa caataattaa gcattcacct
     7201 ttgagaggga ccttttcaat atgctctaaa agttgactaa tggttcctct ttgatactct
     7261 tcatagagtt tcgtcaattc gcgtactaaa acaacttggc gatctccgta aatctctttc
     7321 atgtgtttta gcgtatcaga gactcgaaac ggtgactcat aaaagatttg tgtttcaggg
     7381 taatcttgct ttgtttcaaa gaaagttatt tgttgacctt tcttacgtgg taagaagcca
     7441 taaaaaatat gaggttgtgg agctaaacct gaagcgatga gagcagtaat accagcgcta
     7501 gctcctggta tagatacaac tgggatatcc ccttcaatag cagccttgac aaggtcatgt
     7561 cctgggtcag aaatagaggg cattcctgca tcagatactt gggctaaaga tttcccttct
     7621 tttaacaaat caattaaccc agagatttta tcgtaagcat tgtgttcgtg aaaactaatt
     7681 tgtttagtag taatatcaaa gtgcttgagt aaaagtcccg tatttcgtgt atcctctgca
     7741 caaataaaat caacttctct taaaatccta atggcacgaa aagtcatatc atctagatta
     7801 ccaattggag ttgggactag atagagtgtt ccgtaatgta tatttgattt aaaacttttt
     7861 tgaacttgca tttccatcaa tctctatata ataattcaat acaaaaagca cattcttcgt
     7921 cattttcacg acgttgacca tagaaagtat tacaaatgtg aaagccatca ttgtaaatgc
     7981 cttcgagtaa ttctcttcct tgtttagaat ttttaactgc agtttctgtt tctgcttcaa
     8041 taacgctcag acgctcacga agtttaccat tttcaatgcg tagaacagta ttctcctcca
     8101 ataatttttg gatttgtttt ttcatagtct caatttcaga caacccaact aataaatttt
     8161 gtgaaaaatc atcaaaggca tcaaacaagt cttttttatc cattcactct tcctctctgc
     8221 gcgtattatt ttctaagaaa gcaccatata ctctaaacta ttttgaaaac taacattgct
     8281 cttccacatt tgctgagctc tataagtatt ttctaaatca taaagttggc cttctttagc
     8341 cagcattatt gtcagccatt gaaaaacaaa tgcctgatca tttttatcta caacttgcga
     8401 tgtcaagcga gatacttcta aataagcagt ttgcctatct tttgccaaag tcgtcacaaa
     8461 ctgttgactt aaattcaagt aatctaataa ctttttgtta gtcaacaata tttctaaatg
     8521 tttaggatcg tctgctactt gagctaataa gctagcttgt gattttagca atcctgcttg
     8581 ttcagcttga tgaaccaaca tgtccaattg ttttggaaaa cgaaaaattt gcgttctact
     8641 cttaattgtt ggcaaaacat tgttttcatc attagtcaat aagatgacat aagatgaact
     8701 ttgaggttct tcaataaatt tcaaaagact gttggctgca ttgacatgca ttttttcgca
     8761 atatttgatg ataaagacct gtgatttccc ttcgaaacct gatcttgaaa aatctttagt
     8821 cagctctttt atagtctcta tcttaataag ttgcccctgt ggttctatga ttttaacatc
     8881 tgaaaattcc ccattagcga ttaaacgaca ggctctacac tcttgacatg gtaaaccact
     8941 ctggcgcttt tcacagaact gactttgtgc taaataaagt gccatatcta gacttgcaaa
     9001 atttcccgaa aaaagatagg catgactcat cctatctgac tgtaaaatag tattaaactt
     9061 ttccagcaat ttgggttgtg ttcgctttaa atccatgacc tttgtacttc ctgattcatt
     9121 ggttgatacg atctaaaata atagagaatg tctcagctat tacttcatct agctgttggg
     9181 aggcatcaat cgtcacaatg cggtttggct ccgaatccgc caattcaagg taaccttgac
     9241 ggacgcgttg atgcatatct aattgctcga gatctagacg atttacctcc ctttgaacac
     9301 ttttttgaat acgctctaaa cctacttcag aaggaacatc aaaatataaa gttaaatcag
     9361 gtttatgact atcagtcgca tagtcattaa gccattttat atgtgactta tctaatcctc
     9421 ttcctgatcc ctgataggca acagaactat caataaaacg atccattagg actattttcc
     9481 cttcttctag agctgggagt accttttcca ctagatgttg gcgtcgagca gccatgtaaa
     9541 gcaatagctc tgttttttta tccatttgcg tgtgtttaac atctaaaata atatgacgaa
     9601 tttcttctga aatagttacg ccaccaggct ctctggtggt gagaatatct tgtgataatt
     9661 tttctctgag caagggtaaa acagcttcta aaacggttgt tttacccgca ccatctgggc
     9721 cctcaaaaga aatcattagt ccttttttca tggtacatcc tttatttata attactacta
     9781 ttctatcaaa atttatcaaa aaaagacacc cacaacttga ttgtgcatgt ctgatatttt
     9841 taa
//
LOCUS       DATRQA010000069         9768 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.069, whole genome shotgun sequence.
ACCESSION   DATRQA010000069 DATRQA010000000
VERSION     DATRQA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9768
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.069"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            295..1464
                     /locus_tag="VBD27_001271"
     CDS             295..1464
                     /locus_tag="VBD27_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO7025280.1"
                     /translation="MAWLGNFFTGASFSLVMPFMVLYVEQLGAPSNKVEWYAGLSVSL
                     SALSSALVAPLWGRLADKYGRKPMMVRAGLMMTFTMGGLAFIHSVTGLLILRILNGIF
                     AGYVPNSTALIASQAPQEESGYALGTLATGVTGGMLIGPLLGGLLAEWFGIREVFLLV
                     GTILLISTLMTIFMVKEDFKPISNEETMPTTEVFKSVKSLQILIGLFVTSMIIQISAQ
                     SIAPILTLYIRHLGQTENLMFVSGLIVSGMGFSSILSSPKLGRIGDRIGNHRLLLLAL
                     LYSFLMYVLCSLAQTSLQLGVIRFLYGFGTGALMPSINSILTKIAPRQGLSRIFSYNQ
                     MFSNLGQVLGPFVGSAVSIHLGFRWVFFVTSFIVLANFVWCFINFRKYIRVKEIV"
     gene            1461..1607
                     /gene="rpmG"
                     /locus_tag="VBD27_001272"
     CDS             1461..1607
                     /gene="rpmG"
                     /locus_tag="VBD27_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7025281.1"
                     /translation="MRVKINLKCSSCGSKNYLTSKNKSNHPDKIEVPKYCPKERKVTL
                     HIES"
     gene            1654..1890
                     /gene="secG"
                     /locus_tag="VBD27_001273"
     CDS             1654..1890
                     /gene="secG"
                     /locus_tag="VBD27_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO7025282.1"
                     /translation="MYNLLLTILLVLSVLLIISIFMQPQKNPSSNVFDSSGSEALFER
                     SKARGFEAFMQRFTGVLVFFWLLIGLVLSILSSH"
     gene            2003..4408
                     /gene="rnr"
                     /locus_tag="VBD27_001274"
     CDS             2003..4408
                     /gene="rnr"
                     /locus_tag="VBD27_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO7025283.1"
                     /translation="MKEKIIKYLEEHGKTSVNDLAAALDMAGAKAFPKLIKTISNLES
                     HRQLRFDDNGSLSLQKKEAKKKEITVRGLFRANKAGFGFLSIDQDEDDMFIGKNDIAY
                     AIDGDTVEAVVKKPADRLNGTAAEARVVNIVERSLKTLVGKFVLDDERPKYAGYIKSK
                     NQKINQKIYIRKEPVVLDGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVLE
                     VLESMDIVSEFPEDVIAEANAIPDAPTEKDLIGRVDLRQEVTFTIDGADAKDLDDAVH
                     IKLLDNGHFELGVHIADVSYYVTEGSALNREALSRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNLDRLTQSCIMEIDQNGRVVNHQITQSVINTTYRMTYIAVNDIIAGDEEICSEY
                     ESIVSSVQHMVTLHHTLEAMRTRRGALNFDTSEAKIMVNDKGMPVDIVIRNRGIAERM
                     IESFMLAANETVAEHYARLKLPFIYRIHEEPKAEKLQKFIDYASVFGVQIQGTATKIT
                     QSALQDFMKKVQGQPGSEVLSMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRRY
                     PDLLVHRMIRDYDDKAMDKADHFANLIPEIATQTSSLERRAIDAERIVEAMKKAEYME
                     EYVGEEFEGVVASVVKFGMFVELPNTIEGLIHVTTLPEYYHFNERTLTLQGEKSGKVF
                     RVGQQIKVKLIRSDKETGDIDFDYLPSDFDIVEKVSKSSREGRPNRSSKREHQHRISD
                     RDNKNKNTSKKKASRKPKRNSDSKSHHHKDDRTTGSTKKKTKKPFYKGVAKKGQKRKS
                     KKG"
     gene            4411..4878
                     /gene="smpB"
                     /locus_tag="VBD27_001275"
     CDS             4411..4878
                     /gene="smpB"
                     /locus_tag="VBD27_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO7025284.1"
                     /translation="MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARI
                     TLKDGYAQIKNGEAWLINVHITPYDQGNIWNQDPDRTRKLLLKKREIEKISNELKGTG
                     MTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYNSR"
     gene            complement(4962..6350)
                     /locus_tag="VBD27_001276"
     CDS             complement(4962..6350)
                     /locus_tag="VBD27_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO7025285.1"
                     /translation="MSKNNNDHTQKSENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SISLTGPSIVLVYAITGAFMFFMMRAIGEMLYQDPNQHTFINFITKYLGPGWGFFSTL
                     SYWISLIFIGMAEITAVGEYVQFWFPEWPSWIIQIVFLAILSSINLIAVKAFGETEFW
                     FAMIKVIAILGLIATGIFMVLTNFDTGHGYHASISNITNHFEWFPKGKLNFFMAFQMV
                     FFAYLAIEFVGVTTSETANPRKVLPKAIQEIPMRIILFYAGSLLAIMAIFPWQQLPVN
                     ESPFVTVFKLAGIKWAAALINFVVLTSAASALNSTLYSTGRHLFQLANESPNALTKAL
                     KLDQLSRQSVPSRAIIASAVIVGASALISVLPGISDAFSLITASSSGVYISIYVLIMI
                     AHWKYRKSPDFMEDGYKMPAYKILSPITLLFFLFVFVSLFLQDSTYIGAIGATIWIIG
                     FGLYSHFKHKSH"
     gene            complement(6479..7405)
                     /locus_tag="VBD27_001277"
     CDS             complement(6479..7405)
                     /locus_tag="VBD27_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7025286.1"
                     /translation="MALLSVIVPCYNEQETVSTFLTEIKKVESEMARYTHFEYIFVND
                     GSTDRTLELLKKAAKQFDNVHYLSFSRHFGKDAALLAGLEHTTGDFITVMDVDLQDPP
                     TLLPEMYLKLQEGYDIVATRRKDRKGEPLIRSLFAKLFYKLINQVSDTKMVDGARDFR
                     LMTKQVVDSILELNEVNRFSKGIFSWIGYDVAYISYENRERIAGKTSWSFFNLLKYSL
                     DGFINFSEIPLAIATWIGTLSSVLSLLAIIFIIIRKLLFGDPVSGWASTVTIVLFMGG
                     IQLLSLGIIGKYISKIFLETKKRPVYIVKEEG"
     gene            complement(7534..7926)
                     /gene="gloA"
                     /locus_tag="VBD27_001278"
     CDS             complement(7534..7926)
                     /gene="gloA"
                     /locus_tag="VBD27_001278"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEO7025287.1"
                     /translation="MPFLHTCIRVKDLDASIAFYQEALGFKEVRRNDFPENQFTLVYM
                     SLEDDPSYELELTYNYDHEAYDLGNGYGHIAVGVDDLETTYDAHQKAGYSVTKISGLP
                     GKPNMFYFIQDPDGYKIEVIRLSQFKAD"
     gene            complement(8014..8688)
                     /locus_tag="VBD27_001279"
     CDS             complement(8014..8688)
                     /locus_tag="VBD27_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7025288.1"
                     /translation="MTKEDIKKQVRRAFDFRMAIRVYNNNDIPKEDMEYILDTAWLSP
                     SSVGLEGWRFLVLDRQTIAKFRDKLKEVAWGAQYQLDTASHFVLLLAEKGAYYNADSM
                     INSLIRRGLGDPAALESRIPLYKSFQENDMKIDSERSLWDWTAKQTYIALGNMMTAAA
                     MIGVDSCPIEGFDYEKVNNILSKEGLIDDKKEAISCMVSFGYRLREPKHSRARKERQE
                     VITWVE"
     gene            8955..>9768
                     /locus_tag="VBD27_001280"
     CDS             8955..>9768
                     /locus_tag="VBD27_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7025289.1"
                     /translation="METYTLSNTLNIPKIGFGTWQLTEGEEAYKAVTHALKVGYTHID
                     TAQIYGNEHSVGRAIRDSGLARESIFLTTKIWNDKHDYHLAKASIDESLQKLGVDYID
                     LLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLEALFETA
                     EIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGTIFENESIKAIAEKYGKS
                     VAQVALRWSLDNGFLPLPKSATPKNIEANLDIFDFQLNEDDIATLIQLDSGIK"
BASE COUNT         3287 a         1586 c         1891 g         3004 t
ORIGIN      
        1 atattattag taagcctagg aattatagtt gttattttaa ttattggcta tttagcagtt
       61 gtagtatttg ctccaaaaac tttccgttta aaaagttaat tttaattgtg gtataataac
      121 agaggcatgt gataacatag gtttagttac ataaaggaaa ttataatgga aataacatct
      181 caaatagatg ttaaatctca ataaaactgc taagatagtt agtggtagaa aagtttagaa
      241 agaagatgat gttttatctc agttgaaacg gttaactgga agcaaaattt aaaagtggct
      301 tggctcggta atttttttac tggggcaagc ttttcacttg ttatgccttt tatggtatta
      361 tatgtagaac aattaggagc acctagcaac aaagtagagt ggtatgcagg cctatcagta
      421 tccttatcag ccttgtcttc agctttagtt gccccattat gggggcgctt agctgataaa
      481 tatggtagaa aaccgatgat ggtaagagca gggttgatga tgacttttac tatgggaggc
      541 cttgctttca ttcatagtgt tacaggactt ctgattttac gtatattaaa cggcattttt
      601 gcaggctatg tacctaactc tacagctttg atagcaagtc aagctcctca agaggaatca
      661 ggatatgcct taggaacctt agcgacagga gtgactggag gtatgttaat tggtccactt
      721 ttagggggac ttttagcaga gtggtttggt attagggaag tttttttatt agtgggaacc
      781 atattgttaa tatctacttt aatgaccatt tttatggtaa aggaagattt caaacccatt
      841 tctaacgaag aaaccatgcc aactacagaa gtgttcaaaa gcgttaaaag cttacaaatt
      901 ttaatagggc tgtttgtaac aagcatgatt attcaaattt ctgcacaatc aattgcacct
      961 attttaacac tttacattcg tcatttagga cagactgaaa acttgatgtt tgtttccgga
     1021 ttaattgttt ctggtatggg attctctagt attctgagta gtccaaagct aggaagaatc
     1081 ggggatcgca taggcaatca tcgtttgcta ttattagctc tactgtatag tttcctcatg
     1141 tatgtcttat gttcattggc acagacttca ttacagctag gggttattag attcctgtat
     1201 ggttttggaa caggtgcctt gatgccgagt attaattcaa tcttgactaa aattgctcca
     1261 agacaaggtt tatcaaggat tttcagttat aaccaaatgt ttagcaattt aggacaagta
     1321 ttaggtcctt ttgtcggttc agcagtttct attcatttag gattccgatg ggttttcttt
     1381 gtgactagct tcatcgtttt agctaatttt gtttggtgtt ttattaattt tagaaaatat
     1441 attagagtta aggaaattgt gtgagagtta aaattaattt aaagtgtagc tcttgtggta
     1501 gtaagaatta tttaaccagt aagaataaaa gtaatcatcc cgataaaatc gaggttccaa
     1561 agtattgccc aaaggagaga aaagtaacac tacatattga atcttaataa gttttatgtt
     1621 agaatgtact ttgaccatag tggaggaatt aacatgtata acctattact caccattcta
     1681 cttgttttat cagtattatt gattatctca attttcatgc aaccacaaaa gaatccaagt
     1741 agtaatgttt ttgatagtag cggttctgaa gccctttttg aacgtagtaa agctcgagga
     1801 tttgaagctt ttatgcaacg ttttacaggt gttttagtct ttttctggct actaatcggc
     1861 ttagtattat ctattttatc aagtcattaa tgatagctaa atacagataa gtgaggatgg
     1921 gggatagccc aacctcactt tttgttagta gaatggttgt agagtaataa taaagtgact
     1981 aaagttacta gaaaaggaaa taatgaaaga aaaaattata aaatatttgg aagaacacgg
     2041 taaaaccagc gttaatgatt tagctgctgc cctagatatg gcaggcgcaa aagcatttcc
     2101 aaaattaatc aaaacaatct caaatttgga gagccacagg caattaagat ttgacgataa
     2161 tggaagtctt tctcttcaaa agaaagaagc taagaaaaag gaaatcacag taaggggtct
     2221 ttttagagca aataaagcag gatttggttt tcttagtatt gaccaagacg aagatgatat
     2281 gtttatcgga aaaaacgata ttgcttatgc tattgatggt gacacggtag aagctgttgt
     2341 aaagaaacca gcggaccgtc ttaacgggac tgcagcagaa gcgcgtgttg ttaatattgt
     2401 tgaacgtagt ttaaagacgc ttgttggtaa gtttgtctta gacgatgagc gccctaaata
     2461 cgcaggatat atcaaatcga aaaatcaaaa aatcaatcaa aagatttata tacgcaaaga
     2521 accggtcgtt ttagacggaa cagaaattat caaagtagat attgataaat atccgacacg
     2581 aggacatgat tacttcgtag caagtgttcg tgatatcgtt ggacatcaag gagatgttgg
     2641 tattgatgtt ttagaagtct tggaaagtat ggatatcgtc tcagaatttc ctgaggacgt
     2701 tattgcagaa gccaatgcta tcccagatgc tccaacggag aaagatttaa ttgggcgtgt
     2761 tgatttacgt caagaggtca ctttcactat tgatggagcg gacgccaaag atttagacga
     2821 tgctgtgcat attaaattac tggataatgg tcattttgaa cttggagttc atatcgcaga
     2881 tgtatcatac tacgttactg aaggctctgc cttaaatcgt gaagcactta gtcgtggtac
     2941 gtccgtatat gtcacagatc gcgttgtacc aatgttacca gagcgtctat ctaacggtat
     3001 atgttcatta aatccaaatc ttgatcgttt aacgcagtct tgtattatgg aaattgacca
     3061 aaatgggaga gttgttaatc atcaaattac acagtcagtg attaatacca cttatcgtat
     3121 gacttatatc gctgtaaatg atattattgc tggcgatgag gaaatctgct cagaatatga
     3181 gtctattgtg tcctcagtac agcatatggt cactttacac catactttgg aagctatgcg
     3241 tacgcgtcgt ggagcgctga attttgacac atctgaagca aaaattatgg ttaatgataa
     3301 gggaatgcca gttgatattg ttattcgcaa cagaggcatt gctgaacgca tgattgagtc
     3361 atttatgttg gcggcaaatg aaacggttgc tgaacattat gctcgtctaa aactgccatt
     3421 tatttatcgt atacacgaag agcctaaagc tgaaaaatta caaaaattca tcgattatgc
     3481 ttctgttttc ggtgttcaga ttcaaggaac agcaactaaa atcacccaat ctgctttaca
     3541 ggactttatg aaaaaagtac aagggcagcc aggaagtgaa gtgttatcta tgatgttatt
     3601 gcgctcgatg caacaagcta gatattctga acacaaccat ggacattatg gacttgctgc
     3661 ggaatattac actcacttta ctagccccat tcgacgttat ccagacttgt tagttcatcg
     3721 tatgattcgt gattatgatg ataaagcgat ggataaagct gatcattttg ctaaccttat
     3781 cccagaaatt gcaactcaaa cgtctagtct tgagagacgt gccattgatg ctgaacgtat
     3841 tgtagaggca atgaagaaag ccgaatatat ggaagaatat gttggtgagg aatttgaagg
     3901 tgtggttgca agcgttgtta aatttggtat gtttgtagaa ttaccaaata ctattgaagg
     3961 attgattcac gtaacgacct tgcctgaata ttatcacttt aatgaacgta cgctgacgct
     4021 tcaaggtgag aaatctggga aagttttccg tgttggtcaa caaattaagg ttaagttgat
     4081 tcgttctgat aaggaaactg gtgatatcga ttttgattat ttaccatcag attttgatat
     4141 tgttgaaaag gtaagtaaaa gctcgcgtga gggtcgtcct aatagaagtt ctaaacgaga
     4201 acatcagcat agaatttctg atcgtgataa taagaataaa aatacatcta agaaaaaagc
     4261 atctcgaaaa cccaaaagga actcggatag caaatcacat catcataaag acgatagaac
     4321 aacaggttca accaagaaaa aaacgaaaaa acctttctat aaaggcgttg ctaagaaagg
     4381 tcagaaacgg aaatctaaga aaggttaagt atggttaaag gtcaaggaaa cgttgtagca
     4441 caaaataaaa aagcgcatca cgactatact atcgttgaaa caatagaagc aggaattgtt
     4501 ttgacaggaa cagaaatcaa aagtgtccgc gcagcgcgta ttacattgaa agatggatat
     4561 gcacaaataa aaaatggtga agcgtggttg attaatgttc atattacacc atatgatcaa
     4621 ggtaacatat ggaatcaaga tcctgatcgt acacgaaagt tattactaaa aaaacgtgag
     4681 attgaaaaaa tatcaaatga actgaaaggg actggtatga ctctggtacc tctaaaagtt
     4741 tacttaaaag atggttttgc taaagttcta ttgggtttgg caaaaggaaa acatgattat
     4801 gataagcgag agtctatcaa acgtcgtgag caaaaccgtg atattgcccg tcagttaaaa
     4861 aactataatt cacgatgatt atcaaaaggt tagggaattg cctaaccttt tttggacgaa
     4921 aaaagtgaag ttgattgacc aacttcacta ataatataga cttaatgtga tttatgtttg
     4981 aagtgactat ataaaccaaa tccaataatc cagatggttg ctccaatagc cccgatgtat
     5041 gttgaatctt gcaagaagag agaaacgaat acaaagagga agaagagaag tgtgattggt
     5101 gaaaggattt tgtaggcagg cattttataa ccatcttcca taaaatcagg tgatttacga
     5161 tatttccaat gtgcaatcat aataagaaca tagatagaaa tgtaaacacc agaagatgat
     5221 gcagtaatta aggagaaggc atctgaaata ccaggtaaaa cactgataag tgctgaagct
     5281 ccaacgatta cagctgatgc aataatagct cgacttggaa cgctttgacg agataattgg
     5341 tctaatttta acgcttttgt aagagcattt ggtgattcat tagccaattg gaataaatga
     5401 cgccctgttg agtacaaagt agaattaaga gcagatgctg cagacgttag gacaacaaag
     5461 ttaatcaaag cggcagccca cttaatccca gctagtttga atactgtaac gaaaggtgac
     5521 tcattaactg gtaactgttg ccatgggaag atggccataa ttgctaataa tgaacctgcg
     5581 tagaataaaa taatacgcat cggaatttct tggattgctt ttggtaaaac tttacgtgga
     5641 ttagctgttt cagaagttgt tactccgaca aattcaatag ctaaataagc aaagaatacc
     5701 atttggaaag ccatgaagaa attcaattta cctttaggga accattcaaa atgattggtg
     5761 atatttgaga ttgaagcatg gtatccgtga ccggtgtcaa agtttgtcaa taccataaag
     5821 ataccggttg ctattaaacc aagaattgca ataactttta tcatagcaaa ccaaaactcg
     5881 gtttctccga atgcttttac agcaattaag ttgatagaac ttaggatagc taaaaaaaca
     5941 atttggataa tccaggatgg ccattcaggg aaccaaaatt gaacatactc tcctacggct
     6001 gtaatctctg ccataccgat aaatattagg gaaatccaat aagaaagtgt tgagaaaaat
     6061 ccccaaccgg gtcctaaata tttggtaatg aaattaataa aagtatgctg atttggatct
     6121 tgataaagca tctctccaat agcacgcatc atgaagaaca tgaaagcgcc agtgatagca
     6181 tagacaagaa ctattgaagg acccgtcagg ctaattgaac gacctgcacc aagaaaaagc
     6241 ccagttccga ttgtccctgc aatcgcaatt aactgtacgt gacgattttc taggccgcgt
     6301 accataccat tctcagattt ttgagtgtgg tcattattat tttttgacat tataaatact
     6361 ccatctattt ttgaaaaact ccttcattat ataatttttt tgcaaaattc gcaataatta
     6421 ctatacaata tttttctatg ataaaatctc ctagctaagt gagctaagag atttcaattc
     6481 atccctcttc cttgacaata tagactgggc gttttttagt ttctaggaaa atcttagaga
     6541 tgtatttgcc tataattcct agcgacaata actgaatacc acccataaaa agtacaatgg
     6601 taactgtgct tgcccaacct gaaacgggat ccccaaatag taattttcga attataataa
     6661 aaatgatagc taaaagtgat agtactgaac taagggttcc aatccaggta gctatagcta
     6721 atggaatttc tgagaagttg atgaaaccgt ctagagagta ttttaagaga ttaaagaaac
     6781 tccatgaggt cttacccgca attcgttctc gattctcata actaatataa gcgacatcat
     6841 atccaatcca ggaaaaaatc ccttttgaga agcgattcac ttcatttagc tcgagaatac
     6901 tgtcaacaac ttgttttgtc atgagacgaa aatcacgcgc accatcaacc attttagtat
     6961 ctgacacttg attgattaat ttataaaaaa gcttggcaaa aagagagcgt atcaacggct
     7021 cccctttacg atcttttcgc cttgtagcga caatatcata accttcttga agttttaggt
     7081 acatttctgg taataaagtg ggtggatctt gcaagtcaac atccataaca gtgataaaat
     7141 caccagtagt atgttctaaa ccagccaata aagcagcatc tttaccaaaa tggcgtgaaa
     7201 atgaaaggta atgaacattg tcaaattgtt tagctgcttt cttaagaagt tcgagtgtac
     7261 gatcggtaga accatcgttt acgaaaatat attcaaaatg ggtgtaacga gccatttcgg
     7321 attcaacttt tttgatttct gttaaaaaag ttgagacagt ctcttgttca ttataacaag
     7381 ggacaataac tgataataaa gccatttgta attctccgta tcattcttgc ttaaatttta
     7441 acataatatg ggaaaaaata gaaagattat aaggtaatca taaaaaggac taggtttttg
     7501 cctcagtcct taacttaata tgatgagatg ttattagtca gctttaaatt gactgagtcg
     7561 aataacttca attttatagc catctggatc ttgaataaag tagaacatat taggtttacc
     7621 tggtaatcca gaaatttttg ttacagagta acctgctttc tgatgggcat cataagtagt
     7681 ttccaaatcg tctacaccaa cagcaatgtg accataccca tttcctaaat cataagcttc
     7741 gtgatcatag ttataggtta actctaattc gtaactaggg tcgtcctcta gggacatata
     7801 gactaaagta aattgatttt caggaaagtc attacgacga acttccttaa aaccaagtgc
     7861 ttcttgataa aaagcaattg aggcgtccaa atctttaacg cgaatacaag tatgtaaaaa
     7921 tggcatagtc tctctcctat ttctttatta aacgtattat gtcaaaataa aattatcttt
     7981 tcaaaaatat tgattataaa ttaggctgtc tatttactca acccaagtaa tgacctcttg
     8041 acgttctttc ctagcacggg aatgtttagg ttcacgcaaa cgataaccaa atgaaaccat
     8101 acaagaaata gcttctttct tatcgtctat taacccttct ttactaagaa tattattgac
     8161 cttttcatag tcaaatcctt caattgggca tgaatctacg ccgatcatag cagcagccgt
     8221 catcatatta ccaagtgcga tataagtttg ttttgctgtc caatcccaaa gagaacgttc
     8281 agaatcaatt ttcatatcgt tttcttggaa ggatttgtag agaggtatcc tactctcaag
     8341 ggcggctgga tcacctagac cacgtcgaat gagactgtta atcatgctat cagcgttata
     8401 ataagcacct ttttcagcta gtagtaaaac aaagtgacta gcagtatcta gttgatattg
     8461 tgctccccaa gcaacttcct taagtttatc tcgaaatttt gcaattgttt gtctatctag
     8521 tactaaaaat cgccagcctt ctaaaccgac tgaagaagga ctcaaccaag cagtgtctaa
     8581 aatatactcc atatcttctt taggaatatc attattatta taaacacgaa tcgccatacg
     8641 aaaatcaaaa gctcttcgaa cttgtttttt gatatcttct tttgtcattt tagatcctcc
     8701 tgttattaat gctatcatat cacaatttag aaaaaatctc agcaggcggt atcgtggcaa
     8761 agtagagcta caaaattcta atgactgtca gaaaaataaa gggaatgaac aaaatgatag
     8821 ggttttgatg aagaaagtta tataatttta cataaggagg ggataatacc atatatcaaa
     8881 ctatcattta gatgtttaaa atagatgttt ttcactatga aattcatttt tgtcacatag
     8941 aaaaggagat tgttatggaa acatatactc tatcgaatac tcttaacatt cctaaaattg
     9001 gttttgggac atggcaacta actgaaggtg aagaagctta taaagcagtg actcatgctt
     9061 taaaagttgg ttatactcat attgatactg ctcagattta tggtaatgaa catagtgttg
     9121 gacgtgctat aagagacagt ggtttggcgc gagaaagtat ctttttgaca actaagattt
     9181 ggaatgacaa acatgactat catttagcca aagcttctat tgatgaatct ctgcagaaat
     9241 taggtgttga ttatattgat cttcttttga ttcattggcc aaatccaaaa gctcttcgtg
     9301 aaaatgatgc gtggaaagct gggaatgcag gcacttggaa agcgatggaa gaagcttata
     9361 aagaaggaaa ggtaaaagct attggtgttt cgaatttcat gaaacatcac ttagaggctt
     9421 tatttgaaac cgccgaaata aaacctatgg ttaatcaaat tatcctagca ccaggttgtg
     9481 ctcaagaaga tttggttcgc ttctgtaaag gaaatgatat cttattagaa gcttacagtc
     9541 catttggaac aggtactatt tttgaaaatg aatctataaa agctattgct gaaaaatatg
     9601 gtaaatcagt tgcacaagta gctcttcgct ggtcacttga taatggcttt ttaccattac
     9661 ctaagtcagc gactcccaaa aatattgaag caaaccttga tatttttgat tttcaattaa
     9721 acgaagatga tattgctacc ttaatacaac tagattcagg tattaaac
//
LOCUS       DATRQA010000070         9719 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.070, whole genome shotgun sequence.
ACCESSION   DATRQA010000070 DATRQA010000000
VERSION     DATRQA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.070"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..195)
                     /locus_tag="VBD27_001281"
     CDS             complement(<1..195)
                     /locus_tag="VBD27_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1694 domain-containing protein"
                     /protein_id="HEO7025290.1"
                     /translation="MTELDNKILQKASGENRLDPDQQRYYLGTYKERVVLHLSLKEAQ
                     EEKFQLAITSALNNLILEYPN"
     gene            393..1700
                     /gene="eno"
                     /locus_tag="VBD27_001282"
     CDS             393..1700
                     /gene="eno"
                     /locus_tag="VBD27_001282"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO7025291.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNVIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYSKFEGEGGAVRTAAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIK
                     EEAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(1808..2872)
                     /locus_tag="VBD27_001283"
     CDS             complement(1808..2872)
                     /locus_tag="VBD27_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7025292.1"
                     /translation="MKRKQFIKLGIATLLTVISLYTPINLATNHTTENIVTAQEYKTK
                     ENGTLPFKHKRQLVLGELDDKGRATFAHIQLKVKDEPKKKRVKRLKTTPVGWHNFKFY
                     YNDGTQKAWLMSRGRLICHQFSGLNNERKNLVPMTNWLNTGNYNSTNSSNPESMLFYE
                     KQLKTWLSTHKNYYLDYKVTPIYQNNELIPRKIELKYVGIDKTGKLLPIFIGNKSTQD
                     QFGISTVTLENTSPNATIDYLSGKAQNTVLSAKEQRKLIAKHEEEKRLAEKKVEEEKA
                     AAETQKKLEEEQARLAAEAQRKQKEEQARLAAETQKKQETLVQEQTSQGYKRDYRGRW
                     HRPNGQYASKAEIAAAGLQW"
     gene            3101..4384
                     /gene="aroA"
                     /locus_tag="VBD27_001284"
     CDS             3101..4384
                     /gene="aroA"
                     /locus_tag="VBD27_001284"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO7025293.1"
                     /translation="MKLLTNANTLKGTIRVPGDKSISHRAIIFGSISQGVTRIVDVLR
                     GEDVLSTIEAFKQMGVLIEDDGEIITIYGKGFAGLTQPNNLLDMGNSGTSMRLIAGVL
                     AGQEFEVTMVGDNSLSKRPMDRIALPLSKMGARISGVTNRDLPPLKLQGTKKLKPIFY
                     HLPVASAQVKSALIFAALQTKGESLIVEKEQTRNHTEDMIRQFGGHLDIKDKEIRLNG
                     GQSLVGQDIRVPGDISSAAFWIVAGLIIPNSHIILENVGINETRTGILDVVSKMGGKI
                     KLSSVDNQVKSATLTVDYSHLQATHISGAMIPRLIDELPIIALLATQAQGTTVIADAQ
                     ELKVKETDRIQVVVESLKQMGADITATADGMIIRGNTPLHAASLDCHGDHRIGMMIAI
                     AALLVKEGEVDLSGEEAINTSYPNFLEHLEGLVNA"
     gene            4377..4889
                     /locus_tag="VBD27_001285"
     CDS             4377..4889
                     /locus_tag="VBD27_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO7025294.1"
                     /translation="MPKVLLGFMGVGKTSVANCLENEVIDMDSLIEKHIGMSISRFFT
                     EEGEASFRALESQFLNELLKKKNEGLVIASGGGIVLLEENRRLLTLNRHNNILLTGSF
                     EVLYHRIKKDEKNRRPLFLNHSKEEFYDIYQKRMLLYSGLSDMIIDTDYLTPQKIATV
                     IGESLVNLSN"
     gene            4946..6319
                     /locus_tag="VBD27_001286"
     CDS             4946..6319
                     /locus_tag="VBD27_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7025295.1"
                     /translation="MSRNNYGQLNHHEELRYNYLLKNIHYLNEREKMEFQYLHYKKTA
                     VRPQRRTESPPTNSYYEEPYSDSYYQDDDFYSEPQLTSQGLPIYQEERAPKKKKQRAR
                     KEKQRVKVMAPFPPKAITPPRKKKKFKGFLKFIGIILLIVLSGMVFMFVKGMRDVNNG
                     KSHYSPAIIEDFKGKDAVDGTNILILGSDKRVSERSTDARTDTIMVANVGNKDNKVKM
                     VSFMRDLLVNIPNYSTEGYYDMKLNASFNLGEQDNHKGAEYVRQTLKNHFDIDIKYYV
                     MVDFETFADAIDTLFPNGVKINAKFGLVGGQSADSVKVPDDLRMKNGVVPSQKIKVGI
                     QYMDGRTLLNYARFRKDDDGDFGRTQRQQQVMRAIVSQIKDPRRLFTGSAAIGKAYAL
                     TSSNLSYSFVLTDGIPILSDAKNGIKQMTIPREGDWVDDYDQYGGQGLTIDFAKYKKI
                     LKKMGLR"
     gene            6420..7775
                     /gene="rlmD"
                     /locus_tag="VBD27_001287"
     CDS             6420..7775
                     /gene="rlmD"
                     /locus_tag="VBD27_001287"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO7025296.1"
                     /translation="MLHKNDIIETEISDISHEGMGIAKVDGFVFFVENALPGEIIKMR
                     VLKLRKRIGYGKVEEYLTTSPHRNEGLDYTYLRTGIADLGHLTYEQQLLFKQKQVADN
                     LYKIAHISDVLVEPTLGMTIPLAYRNKAQVPVRRVDGQLETGFFRKNSHTLVSIEDYL
                     IQEKEIDALINFTRDLLRKFDVKPYDEEQQSGLIRNLVVRRGHYTGQLMLVLVTTRPK
                     IFRIDQMIEKLVSAFPSVVSIMQNINDRNSNVIFGKEFRTLYGSDTIEDQMLGNTYAI
                     SAQSFYQVNTEMAEKLYQKAIDFSDLNSEDIVIDAYSGIGTIGLSVAKQVKHVYGVEV
                     VEKAVSDAKENATRNGITNSTYVADSAENAMAKWLKEGIKPTVIMVDPPRKGLTESFV
                     YSAAQTKADKITYISCNSATMARDIKLFEELGYHLVKIQPVDLFPMTHHVECVALLVK
                     A"
     gene            7883..8104
                     /locus_tag="VBD27_001288"
     CDS             7883..8104
                     /locus_tag="VBD27_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025297.1"
                     /translation="MPTMQLLDELVLPKDITIADVSQSIQEFEIERDALQEFFDDMLK
                     TYTTYQEMKDYIKSREANTPKQETEEKSL"
     gene            8222..8959
                     /gene="aphA"
                     /locus_tag="VBD27_001289"
     CDS             8222..8959
                     /gene="aphA"
                     /locus_tag="VBD27_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEO7025298.1"
                     /translation="MKKVLVSSLLVLGITITLQTVVEAKGPKVAYTQEGMTALSDTNK
                     DKVTTISIDEIQKSLEGKKPITVSFDIDDTLLFSSQYFQYGKEYVTPGSFDFLHKQKF
                     WDLVAKRGDQDSIPKEYAKKLIAMHQKRGDKIVFITGRTRGSMYKEGEVDKTAKALAK
                     DFKLDKPIAVNYTGDKPKKPYKYDKSYYIKKYGSDIHYGDSDDDIHAAREAGARPIRI
                     LRAPNSTNLPLPEAGGYGEEVLENSAY"
     gene            9280..>9719
                     /locus_tag="VBD27_001290"
     CDS             9280..>9719
                     /locus_tag="VBD27_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEO7025299.1"
                     /translation="MRVYENKEELKKEISKTFEKYIMEFNNIPENLKDKRIDEVDRTP
                     AENLSYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSIYAMIDLLSEEELFEAHMRKWADEATKTATWEVY"
BASE COUNT         3242 a         1499 c         1894 g         3084 t
ORIGIN      
        1 attaggatat tcaagtatca gattattaag agcagatgtt atagcaagtt gaaacttttc
       61 ttcttgtgcc tcttttaaac ttaagtggag cacaactctt tctttataag taccaaggta
      121 gtaacgttgt tgatcaggat caagacgatt ttcaccgctt gctttttgta ggattttatt
      181 gtcaagttca gtcataattt ctccttgaat ttgctaattt tattataacc taaatgggtt
      241 gcattagcac aggtaaactt gaaaagaatt agtgattttt ttatcaaaaa ccacgtaata
      301 aagcggtttc actgggtttt tccttgttat taactaaaga ttatgatatt atgaatatgt
      361 aaaaaattaa aactcataag gagagtattg taatgtcaat tattactgat gtttacgcac
      421 gcgaagtcct tgactcacgc ggtaacccaa cacttgaagt agaagtttat acagaatcag
      481 gtgctttcgg acgtggtatg gttccttcag gagcttctac tggtgaacac gaagcagttg
      541 aacttcgcga cggcgacaag tctcgttacg gtggtcttgg aacacaaaaa gctgttgaca
      601 atgtaaataa cgttattgca gaagcaatca ttggttatga tgtacgcgac caacaagcta
      661 tcgaccgtgc tatgatcgct cttgacggaa ctcctaataa aggtaaatta ggtgcaaatg
      721 ctattcttgg tgtttctatt gccgtagcac gtgctgcagc tgattacctt gaagtaccac
      781 tttacagcta ccttggtgga ttcaacacta aagttcttcc aactccaatg atgaacatca
      841 tcaacggtgg ttctcactca gacgctccaa tcgcattcca agaattcatg attatgccag
      901 ttggtgcacc aactttcaaa gaagcacttc gttggggtgc tgaagtattc catgctctta
      961 agaaaatcct taaagaacgt ggtcttgaaa cagctgttgg tgatgaaggt ggatttgcac
     1021 ctaaatttga aggtactgaa gatggcgtag aaactattct taaagctatt gaagcagctg
     1081 gttacgaagc tggcgaaaac ggaatcatga ttggttttga ctgtgcttca tcagagttct
     1141 acgatgcaga acgtaaagta tacgactaca gtaaattcga aggtgaaggt ggagcagttc
     1201 gtactgctgc agaacaaatc gattaccttg aagagttggt taacaaatac ccaatcatca
     1261 ctattgaaga tggtatggat gaaaacgact gggatggttg gaaagctctt acagaacgcc
     1321 ttggtggacg tgtacaatta gttggtgacg atttcttcgt tacaaatact gattacttag
     1381 cacgtggtat caaagaagaa gctgctaact caatccttat caaagttaac caaatcggta
     1441 ctttgacaga aactttcgaa gctattgaaa tggctaaaga agctggttac actgcagttg
     1501 tatcacaccg ttcaggtgaa actgaagatt caacaatcgc tgatatcgca gttgcaacta
     1561 acgctggtca aattaaaaca ggttcattat cacgtactga ccgtattgct aaatacaacc
     1621 aattacttcg tatcgaagac caacttggtg aagttgctca atacaaaggt atcaaatcat
     1681 tctacaactt aaaaaaatag tctcagatta ttaaacagtt gaaaatataa taaaaaccat
     1741 cttgcatatg caagatggtt tttatgtaca cattataata atagaattga tttgataata
     1801 aacagttcta ccattgcaat ccagcagcag cgatttctgc ttttgaagcg tattgtccat
     1861 ttggacgatg ccaccttcct ctatagtcac gtttgtagcc ttgactagtt tgttcttgta
     1921 ctagagtttc ttgttttttc tgagtttcag ctgcgagcct tgcttgctcc tctttttgct
     1981 tcctttgagc ttcagcagca agccttgctt gttcttcttc cagttttttc tgagtttcag
     2041 cagcagcttt ttcctcttca actttctttt ctgcaagtcg tttttcttct tcgtgcttag
     2101 cgataagttt tctttgttct ttagctgata agacagtatt ttgagccttt ccgctgaggt
     2161 agtcaatggt agcatttggt gaggtattct ctaaggtaac agtgctgatg ccgaattgat
     2221 cttgagtgga tttgtttccg ataaaaatag gtaataattt cccagtctta tcaataccaa
     2281 catattttaa ttcaattttt cttggaatta gttcattatt ttgataaatt ggtgttactt
     2341 tataatctaa gtagtagttt ttatgagtcg aaagccaagt ctttagttgt ttttcataga
     2401 atagcatgct ctcagggtta gatgaattcg tgctattgta gtttccagta tttagccaat
     2461 tcgtcattgg aactaaattc tttctttcat tgtttagtcc actaaattgg tggcaaataa
     2521 gacggcctcg gctcattagc caagcttttt gagtaccatc attataatag aatttaaaat
     2581 tgtgccatcc aacaggggtt gtttttaaac gttttactct ctttttcttg ggttcatctt
     2641 taacttttag ctgaatatgt gcgaatgtgg cacgaccttt gtcatccaac tctcctaaaa
     2701 ccaattgtcg tttgtgttta aaaggtaaag taccattctc ttttgtttta tactcttgag
     2761 cagtaacaat attttctgtg gtatgatttg tagctaggtt tattggtgtg taaagcgaaa
     2821 taaccgttag taaggttgca attcctaatt ttataaactg ttttcttttc atctgttaac
     2881 ctctaaaata acataaaata gtagctgttt atttttaaat gcattctgaa aattaaaatg
     2941 tgatttttgt aacaatatta taacataatg atgtatgaca tgtgaagtta atttataaaa
     3001 gatgttacag ctatatagag gagctatagt ataaataaaa aattcaaaat atagcatata
     3061 ttatgataca ataatagcaa aatagatttt gggagttatc atgaaattac tgactaatgc
     3121 aaatactctc aagggaacca tccgtgttcc tggcgataaa tcaataagtc acagggctat
     3181 tatttttggg agtatatctc agggagttac ccgaattgtt gatgtcttaa gaggcgagga
     3241 tgttttatca actatagagg cttttaaaca aatgggggtt ctaattgaag acgatggcga
     3301 gataatcact atctatggca aaggatttgc aggtttaaca caaccgaata atctcttaga
     3361 catgggaaac tctggtactt caatgcgtct gatagcaggt gtgttagctg gtcaagagtt
     3421 tgaagtgacg atggttggag acaatagttt gtcaaagaga cctatggatc gtatagcatt
     3481 gcctttaagc aaaatgggag ctagaatttc gggagtaact aaccgtgatt taccaccatt
     3541 aaaacttcaa ggtacaaaaa aattaaaacc tattttttac catttacccg tagcttctgc
     3601 acaagtaaaa tcagctttaa tttttgcggc tttacaaact aagggagaat ctctcattgt
     3661 agaaaaagag caaacaagga atcatactga ggacatgatt cggcaatttg gtggacattt
     3721 ggatattaaa gataaagaaa ttcgactcaa tggcggtcag agcttagtag gacaagatat
     3781 aagagttcct ggtgatattt cgagtgctgc cttttggata gtagcgggtc ttattattcc
     3841 taacagtcat attattttag aaaacgtagg tataaacgag acacgaactg gaatccttga
     3901 tgtcgtcagt aaaatgggtg gaaaaattaa gttatcttcg gttgataatc aagtaaaatc
     3961 agcaaccctt acagttgatt actctcatct acaagctact catatttctg gggctatgat
     4021 tccacgtctg attgatgaat tacctattat tgctctttta gcaacacaag ctcaaggcac
     4081 cacagtcata gcagatgctc aggaattaaa ggtgaaagag acagatcgga ttcaggtcgt
     4141 tgtcgaatcg ctaaaacaga tgggtgctga tattactgct acagcagatg gtatgattat
     4201 acgtggtaat acccctcttc acgctgcttc acttgattgt catggagatc atagaatagg
     4261 aatgatgata gctattgctg ctctcttggt caaagaaggc gaagtagatt tatctggtga
     4321 agaagctatt aatacaagct atcctaactt tttagaacat ttggagggat tagttaatgc
     4381 ctaaggtatt attgggattc atgggagttg gcaagactag tgttgccaac tgcttagaaa
     4441 atgaagtaat tgatatggat agtctgattg aaaaacatat tggtatgtca atttcccgat
     4501 tttttacaga agaaggagaa gcctcctttc gtgctttaga atctcaattt ttaaacgaac
     4561 ttttaaagaa aaaaaatgaa ggacttgtga tagctagtgg aggaggtatt gtactccttg
     4621 aagaaaatcg tcgtctttta actttaaatc gtcataataa tattctttta acaggaagtt
     4681 ttgaagtctt atatcatcgt ataaaaaaag atgagaagaa tagaagacca ttatttttaa
     4741 accattcaaa agaagaattt tatgatattt atcaaaaacg catgttactc tatagtggtt
     4801 tgtctgatat gattattgat accgattatc ttacaccaca aaaaatagct actgtaattg
     4861 gagagtcact ggttaattta tctaactaag ttatcacagt ttggtagtat gaactattat
     4921 tattgaaagt aaatcacctg aaaatatgtc aagaaataat tacggtcagc taaatcatca
     4981 tgaagagcta cgatataact atcttttaaa aaacatccat tatcttaatg aacgtgaaaa
     5041 gatggaattt cagtaccttc attataaaaa aacagcagta cgaccacaga gaaggacaga
     5101 aagtccacca actaattctt attatgagga accttattct gattcttatt atcaagatga
     5161 cgatttttac tcagagcctc agctaacctc acaaggttta ccaatttatc aagaagagag
     5221 agctccgaag aagaaaaaac aaagggccag aaaagaaaaa cagagggtga aggttatggc
     5281 gcctttccct ccaaaggcta taaccccccc tagaaagaaa aaaaaattta agggtttcct
     5341 aaagttcata ggtattatat tactaattgt tttatcaggt atggttttca tgtttgttaa
     5401 aggaatgcgt gatgtgaata atgggaagtc acattatagc cctgcaatta ttgaagattt
     5461 taagggaaaa gatgctgttg atgggactaa tatcttaatt ttaggtagtg ataaacgtgt
     5521 cagcgagaga tctactgatg cgagaacaga taccataatg gttgctaatg ttggtaacaa
     5581 ggataataag gttaagatgg tgagttttat gcgagatctc ttagtcaata ttcctaatta
     5641 tagtacggaa ggttactatg atatgaaatt aaatgcgtct tttaatttgg gagaacaaga
     5701 taatcataaa ggagcagagt atgttcgaca aacgttgaaa aatcattttg atattgatat
     5761 aaaatattat gttatggttg actttgagac atttgcagat gctattgata cactgtttcc
     5821 taatggtgtc aagattaatg ctaaatttgg acttgtcggt ggacaatctg ctgattcagt
     5881 taaagttcct gatgatttaa ggatgaaaaa tggcgttgta cctagccaaa aaattaaggt
     5941 tgggattcag tacatggatg gaaggaccct tctaaattat gctcgtttca gaaaagacga
     6001 tgatggggac tttggtcgaa cacaacgtca acaacaagtt atgagagcta ttgtctctca
     6061 aataaaagac ccacgtcgtc tctttacagg ttctgctgct atcggtaaag cctatgcttt
     6121 aacttcaagt aatttatctt atagctttgt cctaacagat ggcattccaa ttttatcaga
     6181 tgctaaaaat ggtattaagc aaatgacaat tccaagagaa ggagattggg tagatgatta
     6241 tgaccagtat ggtggtcaag ggttaacaat tgattttgct aagtacaaaa agatcctaaa
     6301 gaaaatggga ctgcgttaac atttttaatt atgatataat agagtgattt attgtcactc
     6361 tatttttatc tttagtgtat aaactaaaga cttcaaatga tagatataaa aggaaaatta
     6421 tgttacataa aaatgatatt attgaaactg aaattagcga cattagccat gagggaatgg
     6481 gtattgccaa agtagatggc tttgttttct ttgttgaaaa tgctcttcca ggtgagatta
     6541 ttaagatgcg cgtcttgaaa ttacgtaagc gtataggata tggaaaagtt gaggaatatt
     6601 taacgacttc tcctcaccga aatgaaggat tagactatac ctatttacgt acagggattg
     6661 ctgatttggg acatcttact tatgaacaac agctgttatt taaacaaaaa caagtagctg
     6721 ataaccttta taaaattgct catatatctg atgttttagt agaacctact cttggtatga
     6781 caatcccttt ggcatatcga aataaagctc aggtaccagt tcgtcgtgtt gatggacagc
     6841 tagagacagg ttttttcaga aaaaattcac atactctggt atccattgaa gattatttaa
     6901 ttcaggaaaa agagattgat gctctgatta attttacacg agatttatta agaaaatttg
     6961 atgttaaacc atatgacgaa gagcagcaat ctggtttaat tagaaatctc gttgttcgtc
     7021 gaggacatta tacagggcaa ttaatgttgg ttttggtgac aacacgtcct aaaatattcc
     7081 gcattgacca aatgattgaa aagttggtgt ctgcctttcc ttctgttgtt tcaattatgc
     7141 aaaatattaa tgatcgtaat agcaacgtta tttttgggaa agaatttcgt actctatatg
     7201 gttctgatac tattgaggac caaatgttag gaaatactta cgctatatca gcccaatctt
     7261 tttaccaagt taatactgaa atggctgaaa aactgtacca aaaagcaatc gatttttcag
     7321 atttaaactc agaggatatc gttattgatg cctattcagg tataggaaca attggcttat
     7381 ctgttgctaa acaagtaaaa catgtttatg gtgttgaagt tgttgaaaaa gcagtaagtg
     7441 atgccaaaga aaatgctact cgaaatggga taactaacag tacctatgtt gctgattcgg
     7501 ctgaaaatgc aatggcaaag tggcttaaag aaggtataaa accaactgtt ataatggttg
     7561 atccaccacg aaaaggatta acggagagtt ttgtatattc tgctgcccaa acaaaagcag
     7621 ataaaatcac ttatatttct tgtaattcag cgacaatggc gcgtgatatc aaactatttg
     7681 aagaattagg gtatcattta gtaaaaattc aaccagtaga cttattccca atgacacatc
     7741 atgtggagtg tgtggctctg ctcgtaaaag cctagaaact ttttaacgaa aggaatatta
     7801 aaaagcctta gataatcgac tgacctatta ccaaaaaatc gaaggggctc tacttgccat
     7861 tgcacaaaca ccaggaaata gtttgccaac tatgcagctg ttagatgagt tagttcttcc
     7921 aaaagacatt accatagcag atgtcagtca aagtatccaa gaatttgaaa tagaaagaga
     7981 tgcccttcaa gaattttttg acgatatgct gaagacttat acaacctacc aagaaatgaa
     8041 agattatatt aaatctcgtg aagcgaacac tcctaagcaa gagacagaag aaaaatcact
     8101 ttgaagactg tagacttaac ataagtaaaa aataaccata tatgatatcg ctttcattct
     8161 tatgtttttt gtgataaact aattaaagaa ataattttta tactttatta tggaggttct
     8221 tatgaagaaa gtgttagtga gtagtctttt ggttttaggg attacgataa cgttacaaac
     8281 agtagttgag gctaaggggc caaaagtagc ttatacacaa gagggaatga ctgctctttc
     8341 ggacacaaat aaagataaag tcactactat ttctattgac gagattcaaa aaagcttaga
     8401 aggtaagaag ccgattactg ttagttttga tattgatgat acactgcttt tcagtagtca
     8461 atattttcaa tatggtaaag aatatgtaac tcctggatcg tttgattttc ttcataaaca
     8521 aaaattctgg gatcttgttg caaaacgagg agatcaagat tccattccca aagaatatgc
     8581 taaaaaatta attgctatgc atcaaaaacg aggagataaa attgttttta taacaggtag
     8641 gacaagaggg tcaatgtata aggagggcga ggttgataaa acagctaaag ccttagctaa
     8701 agattttaaa ttagacaaac caattgctgt aaattataca ggcgataaac ctaaaaagcc
     8761 atacaaatat gataaatcat attatattaa gaaatatggt tcagacattc attatggaga
     8821 tagtgatgac gatattcatg cagctaggga ggccggtgct agaccaatta gaattttaag
     8881 agcacctaat tctacaaatc tacctttacc agaagctgga ggctacggtg aagaggttct
     8941 cgaaaattca gcttactagt atctttatta gttaacatag tattatctat caatttttag
     9001 atttgtaata agaagtgcta aaaaagtagc aaatcaatga tatgctaata agggaagttg
     9061 gagtagttta gattgattat taatgactga tcttctctcc tctcggtaac aagaagtttt
     9121 tctaacaaat gcctgtctaa aaaaattgaa tcataatttt ttaataactc ttttggcgtg
     9181 aacaaggaat ttgtaatcag tcatctaatt ctataataca atactttggg gttgtttccg
     9241 tatcattatt aataatatgt atcagaaagg agatgttttt tgagagttta tgaaaataaa
     9301 gaagagttga aaaaagaaat aagtaaaaca tttgagaaat acattatgga atttaataat
     9361 attccagaga atctaaaaga taaaagaatt gatgaagttg atagaactcc agcagaaaac
     9421 ctttcttatc aggttggctg gaccaacttg gttcttaaat gggaagaaga tgaaagaaag
     9481 ggacttcaag taaaaacacc atcggataaa tttaaatgga atcaacttgg tgaattatat
     9541 cagtggttca cagataccta cgctcattta tctctgcaag agttgaaagc aaaattaaat
     9601 gaaaatatta attctatcta tgcaatgatt gatttgttga gtgaggaaga attatttgaa
     9661 gcgcatatga gaaagtgggc tgatgaagcg actaaaacag cgacttggga agtgtataa
//
LOCUS       DATRQA010000071         9433 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.071, whole genome shotgun sequence.
ACCESSION   DATRQA010000071 DATRQA010000000
VERSION     DATRQA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.071"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            33..1367
                     /locus_tag="VBD27_001291"
     CDS             33..1367
                     /locus_tag="VBD27_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO7025300.1"
                     /translation="MEDPGSQSLLLQFVILLILTLFNAFFSASEMALVSLNRSKVEQK
                     AEEGDKRYRRLLDVLENPNNFLSTIQVGITFISLLQGASLSASLGHVISGWLGNSATA
                     RTAGSIIALIFLTYVSIVLGELYPKRIAMNLKDRLAIVSAPIIIFLGKIVSPFVWLLS
                     ASTNLLSRITPMTFDDADEKMTRDEIEYMLTNSEETLDAEEIEMLQGIFSLDEMMARE
                     VMVPRTDAFMIDINNDAQSNIEGILSQNFSRVPVYDDDKDRVVGVLHTKRLLEAGFKT
                     GFDTIDLRKILQEPLFVPETIFVDDLLKALRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDTAEQFVREIDENIYIVLGTMTLNEFNDYFETELESDDVDTIAGYYLT
                     GVGSIPNQEEKVAYEVDSKDKRITLINDKVKDGRITKLKVLLSDIEQNIEKD"
     gene            1435..2223
                     /gene="pflA"
                     /locus_tag="VBD27_001292"
     CDS             1435..2223
                     /gene="pflA"
                     /locus_tag="VBD27_001292"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEO7025301.1"
                     /translation="MAEIDYKKVTGMIHSTESFGSVDGPGIRFIIFMQGCKMRCQYCH
                     NPDTWEMETNNSKERTVEDVLKEALRYKHFWGKDGGITVSGGEAMLRIDFITALFIEA
                     KKLGIHTTLDTCGFAYRATPEYHAILEKLLDVTDLVLLDLKEIDPEQHKIVTRQSNKN
                     ILQFARYLSDRGTPVWIRHVLVPGLTDIDDHLKRLGEFVQTLDNVDKFEVLPYHTMGE
                     FKWRELGIPYPLAGVKPPTPERVKNAKDIMKTESYTEYLKRIQN"
     gene            2340..3275
                     /locus_tag="VBD27_001293"
     CDS             2340..3275
                     /locus_tag="VBD27_001293"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEO7025302.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAWGLDTEAVALGTPN
                     EETAYVLDYFGVQAPRVVESAKAEGVETVILTDHNEFQQSISDIKDVTVYGVVDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRMFKENGVSVPKELAGLLLSGLISDTLLLKSPTT
                     HASDIPVAKELAELAGVNLEEYGLEMLKAGTNLSSKTAAELIDIDAKTFELNGEAVRV
                     AQVNTVDINDILARQEEIEVAIQEAIVTEGYSDFVLMITDIVNSNSEILALGSNMAKV
                     EAAFEFTLENNHAFLAGAVSRKKQVVPQLTESYNA"
     gene            3335..3988
                     /locus_tag="VBD27_001294"
     CDS             3335..3988
                     /locus_tag="VBD27_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO7025303.1"
                     /translation="MILTFNPGKLERQEFFKELINYLWIHDDVTLRQIKSHFTDYSKI
                     DRLLEEYINHGYILRQNKRYSLNLPFLSSLDGLALDDLVFIDSASQIYQLLQKRKFVT
                     NLDNQTNHLVFVEETDFERNTLTLSNYFYKLTNCYPLSREQKKLYQLLGDVNSEYALK
                     YMSSFILKFLRKDSVKQKRTDIFIQALELLGYISLNQDTTYRLNAKLDVEALKIYLT"
     gene            complement(4018..5019)
                     /locus_tag="VBD27_001295"
     CDS             complement(4018..5019)
                     /locus_tag="VBD27_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO7025304.1"
                     /translation="MIQKNKAPFVLISSVIILLLLILVSISLGYANTSVIDVLKLISG
                     KSDDAFLFIITNIRLPRIIVCIFGGASLGIAGLLLQTLTKNPLADSGILGINAGAGLV
                     IALTIGTFNVSNPTILYFLPLFAMFGGLVTIFLVYLMSYRRNHNISPTRLIITGIGIS
                     TMISGVMISIISQSNNQKMDMIVEWLSGKITISSWTTIITFIPILILLWGLAYSRSRH
                     LNIMNLNEQTALALGLHLKKERIYTLMLASSLAAISVVLIGNITFIGLLAGHLSRRLL
                     GNNHKIILPSCLLIGAIILLVSDTIGRLLLVGTGIPTGLVVSIIGAPYFLWLMTKVD"
     gene            complement(5016..6041)
                     /locus_tag="VBD27_001296"
     CDS             complement(5016..6041)
                     /locus_tag="VBD27_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO7025305.1"
                     /translation="MFKASKKLVQKNKSNHFWLVFFITLILFLIGCYASLRFGAINFK
                     TSDLITVLKNPLKNSNAQDVIFDIRLPRIIAAILVGAAMSQAGAIMQGVTRNAIADPG
                     LLGINAGAGLALVVAYAFLGSMHYSTILIVCLLGSVISCLLVFTLSYTKQKGYHQLRL
                     ILAGAMISTLFTSVGQVVTLYFKLNRTVIGWQAGGLSQINWKMLIIIAPIIILGLLIS
                     QLLAHQLTILSLNESVAKALGQKTQLMTAFLLLIVLFLSASSVALIGTVSFIGLIIPH
                     FIKLFIPKDYRLLLPLIGFSGATFMIWVDLSSRIINPPSETPISSIISIVGLPCFLWL
                     IRKGKNL"
     gene            complement(6057..6989)
                     /locus_tag="VBD27_001297"
     CDS             complement(6057..6989)
                     /locus_tag="VBD27_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7025306.1"
                     /translation="MKKIGIIVLTLLTFFLVSCRQQTKEESTKTTISKMPKIEGFTYY
                     GKIPENPKKVINFTYSYTGYLLKLGVNVSSYSLDLEKDSPVFGKQLKEAKKLTADDTE
                     AIAAQKPDLIMVFDQDPNINTLKKIAPTLVIKYGAQNYLDMMPALGKVFGKEKEANQW
                     VSQWKTKTLAAKKDLHHILKPNTTFTIMDFYDKNIYLYGNNFGRGGELIYDSLGYAAP
                     EKVKKDVFKKGWFTVSQEAIGDYVGDYALVNINKTTKKAASSLKESDVWKNLPAVKKG
                     HIIESNYDVFYFSDPLSLEAQLKSFTKAIKENTN"
     gene            complement(7013..7807)
                     /locus_tag="VBD27_001298"
     CDS             complement(7013..7807)
                     /locus_tag="VBD27_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025307.1"
                     /translation="MSHIKAENIIVSYDQKEIINNLSLSILNQKITTIIGANGCGKST
                     LLKALTRIHKIKDGTITIDGHDIAHLPTKEIAKKIALLPQVLEATEGITVYELISYGR
                     FPHQKYLGNLTNDDRSKIHWAMEMTNVAQFANRDVDDLSGGQRQKVWIAMALAQDTDT
                     IFLDEPTTYLDMNHQLEVLELLKKLNNETQKTIIMVLHDLNLSARYSDYLVAMKTGKI
                     IYEGSPSQIMTKDIIKDIFKIDAHIIQDPISKQPVLLSYQLSTDQN"
     gene            complement(7966..9417)
                     /locus_tag="VBD27_001299"
     CDS             complement(7966..9417)
                     /locus_tag="VBD27_001299"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEO7025308.1"
                     /translation="MITIDKILEILKNDHNFREILFHEHYYYNWTQNVTFNALSYDSR
                     QISSDTLFFAKGATFKKEYLDSAITAGLSFYVSEIDYGADIPVILVNDIKKAMSLISM
                     SFYNNPQNKLKLLAFTGTKGKTTAAYFAYHMLKVNHRPAMLSTMNTTLDGKSFFKSHL
                     TTPESLDLFRMMATAVENQMTHLVMEVSSQAYLTKRVYGLTFDVGVFLNISPDHIGPI
                     EHPTFEDYFFHKRLLMENSNAVVVNSQMDHFNIVKEQVEYIPHDFYGDYSENVITESQ
                     AFSFHVKGKLENTYNINLIGKFNQENALAAGLACLRLGVSIEDIKNGIAQTTVPGRME
                     VLTQTNGAKIFVDYAHNGDSLKKLLAVVEEHQKGDIILVLGAPGNKGQSRRKDFGDVI
                     NQHPNLQVILTADDPNFEDPLVISQEIASHINRPVTIIIDREEAIANASTLTNCKLDA
                     IIIAGKGADAYQIIKGNRDNYSGDLEVAKKYLK"
BASE COUNT         3083 a         1507 c         1749 g         3094 t
ORIGIN      
        1 ttcaaataat ttttatttag gagtaatttt ccatggaaga ccctggcagt cagagtttgt
       61 tactacaatt tgttatttta ttaatattaa ccctgtttaa cgccttcttt tcagcatctg
      121 aaatggcttt agtatcgcta aaccgctcta aagtagaaca gaaagctgaa gaaggggaca
      181 aaaggtatcg acgtcttttg gacgttcttg aaaatcccaa taatttttta tcaacaattc
      241 aagttggtat tacttttatt agccttttgc aaggggctag tttatctgcc tcactaggac
      301 atgtgatttc aggatggcta ggaaattcag caacagcccg tacagcaggg agtattattg
      361 cgctaatctt tttaacatat gtctctattg ttctaggaga attatatcct aagcgtattg
      421 ctatgaattt gaaagatcgt ctagcgattg tttcagctcc cattattatc tttctgggga
      481 aaatagtaag cccctttgta tggttgctct cagcttcaac taacttattg agtagaatca
      541 ctccgatgac ttttgatgat gctgacgaga agatgacacg tgatgaaatt gaatatatgt
      601 tgacgaatag tgaagaaact ttggatgctg aagaaattga gatgctgcaa gggattttct
      661 cgctagatga aatgatggcg cgtgaagtta tggttccgcg tactgatgct ttcatgattg
      721 acatcaacaa tgatgcacaa tcaaatattg aaggaatttt atcacaaaac ttttctcgtg
      781 tccctgttta tgatgacgat aaagatagag ttgttggtgt tttacatacc aagcgcttat
      841 tggaagcggg ctttaaaact ggttttgata ctatcgactt gcgaaaaata cttcaggaac
      901 ctctttttgt tccagaaacg atttttgtag atgatctttt aaaagcatta cgcaacactc
      961 aaaatcaaat ggccatttta ctagatgaat atggtggcgt tgctggttta gtaactctag
     1021 aggatttatt ggaagaaatt gtcggcgaaa ttgatgatga aacagataca gctgaacaat
     1081 ttgtcaggga aatcgatgag aatatctata ttgttctagg aactatgacg cttaatgaat
     1141 ttaatgatta ttttgagaca gagttagaaa gtgatgatgt tgatacaatt gcgggttatt
     1201 atttaactgg tgtgggtagt attccaaacc aagaggaaaa agtagcttac gaagtagaca
     1261 gcaaagataa acgcatcact ttgattaatg ataaagttaa agatggtcgt ataacaaaat
     1321 tgaaagtgct attgtctgat atagaacaga atattgaaaa agactaagat tacttagtct
     1381 ttttttcatg aaaatgatat aataaagtca taaaaacggt tacaaggtga tgaaatggca
     1441 gaaatagact ataaaaaagt aacaggaatg atccattcta cagaaagttt cggctctgtg
     1501 gatgggccag gtattcgttt tatcattttt atgcaaggat gtaaaatgcg ttgtcaatac
     1561 tgtcataatc ctgatacttg ggaaatggaa actaacaatt caaaggaacg caccgttgag
     1621 gatgttctaa aagaagctct tcgttataaa catttttggg gaaaagatgg aggtatcaca
     1681 gtttcaggtg gtgaagcaat gctgcgaatt gactttatta cagctctctt tattgaggct
     1741 aagaaactag gtattcatac gacgctagat acttgtggtt ttgcttatcg agcaacacca
     1801 gaataccatg ctatccttga aaaattgtta gatgttactg atttggtact ccttgactta
     1861 aaagaaattg atccagaaca acataaaatt gtaactcgtc agtctaacaa aaatatttta
     1921 cagtttgcta gatatttatc tgatagaggt actccggtat ggattcgtca tgttttagtt
     1981 cctggattga ctgatattga tgaccattta aaacgtttgg gtgaatttgt ccaaacactt
     2041 gataatgtcg ataaatttga agtattgcct tatcatacta tgggagagtt caaatggcgt
     2101 gaattaggaa ttccgtatcc attagctggt gtgaaaccac caactccaga acgtgttaaa
     2161 aacgctaaag atatcatgaa aacagaatcc tatacagaat atctaaaaag aattcaaaat
     2221 tagttttaag ttgaatttta ttcaactttt ttagttgcaa agggcttcct agtaagattt
     2281 tttgctaagt tattcaattt ttgctacaat aaggtgaata taaaagaaga ggtaaaaaca
     2341 tgtctaaaat tttagtcttt ggtcaccaaa atccagattc agatgctatt ggatcatcag
     2401 ttgcttttgc atatctagct aaagaagctt gggggcttga cacagaggcc gttgctttag
     2461 gaactccaaa tgaagaaaca gcatatgttt tagattattt tggtgttcaa gcacctcgag
     2521 tagtagaatc tgcaaaagca gagggtgtag agacagtcat tttaactgac cataacgaat
     2581 tccaacaatc tatttctgat attaaagatg tgacagttta tggtgttgtt gatcatcatc
     2641 gtgttgctaa ctttgagact gccaatccgt tatatatgcg tttggaacca gttggctcag
     2701 catcatcgat tgtttaccgt atgtttaagg aaaatggagt atcagtaccc aaagagttgg
     2761 ctggtttatt attatcaggt ttgatttcag atactcttct tttgaaatca ccaacaactc
     2821 atgcttcaga tattccagtt gcaaaagaac tagcagagct tgcaggtgta aacttagaag
     2881 aatatggttt agaaatgctt aaagcaggta ctaacctctc aagtaagact gcagcggagt
     2941 taattgatat tgatgctaaa acatttgaat taaatggaga agctgttcgt gttgcacaag
     3001 ttaatactgt ggacattaac gatattttag cacgtcaaga agagattgaa gttgctattc
     3061 aagaagcaat tgtcacagaa ggttattcgg attttgtatt gatgattact gacattgtta
     3121 attcaaattc agagatttta gctctcggtt caaatatggc taaagttgaa gcagcttttg
     3181 aatttacact tgaaaataac cacgcttttc ttgctggggc agtatcacgt aaaaaacaag
     3241 ttgttcctca attgacagaa agctacaatg cttaattaat catgtaaaac gggctgcgtc
     3301 ccgttttttt agtcatacat ttaggagcgt ttttatgata ttaacgttta atccaggaaa
     3361 gttagaaaga caagagtttt ttaaggagct tattaactat ctttggatac atgatgatgt
     3421 aacactaaga caaataaagt cccactttac agactattct aaaattgacc gtttgttaga
     3481 agaatatatt aatcatggat atatccttcg ccaaaataaa cgctacagcc ttaatcttcc
     3541 ttttctgagt tcgctggatg gattagcatt agatgatttg gttttcatag attctgctag
     3601 tcaaatctat cagttattac agaagcgaaa atttgtgaca aatttagata accaaaccaa
     3661 tcacttggta tttgtagaag agacagattt tgaaagaaac actttgactc tttctaatta
     3721 tttctataaa ctaactaact gttatccact ttcaagagaa caaaaaaagc tttatcaact
     3781 tttaggagat gttaattcgg aatatgcttt aaaatatatg tcaagcttta tattgaaatt
     3841 tcttcgcaag gatagtgtta agcaaaaacg tacagatatt tttatccaag ctttagaatt
     3901 acttggatat attagcttaa accaagatac tacctatcgt ttaaatgcta aacttgatgt
     3961 tgaagcttta aagatttact taacataaaa aaagactgga agtttgtcca gtctttttta
     4021 gtcaactttt gtcatcaacc agagaaaata gggggcacca attattgata caaccaatcc
     4081 agtgggtata cctgtaccaa ctaagaggag acgacctata gtatctgata ccaataggat
     4141 aatagcacca ataagaagac agctaggtag tataatttta tggttatttc ctaatagtcg
     4201 tctacttaag tgcccagcga gtaagcctat aaaagtaata ttaccaatta agacgacgct
     4261 aatagcagca aggctacttg ccagcatcaa agtatatatt ctttcctttt ttaaatgaag
     4321 ccctagagcg agagcagtct gctcgtttag gttcataata ttaaggtggc gactgcgact
     4381 atatgctaat ccccataaca aaatgagaat cgggataaaa gtgataatgg tagtccagga
     4441 actaattgta atttttccac taagccattc aacgatcata tccatttttt gattattaga
     4501 ctgactaata atagaaatca taacgcctga aatcattgta gatattccga taccagtgat
     4561 gattaatcgt gtagggctaa tattgtgatt tcttctgtat gacataagat agacaagaaa
     4621 aatagttacc aaccctccaa acatagcaaa taggggtaaa aagtataata tagttggatt
     4681 gctaacatta aatgttccta tggttagagc gattactaat ccagctccag cattgatacc
     4741 aagaataccg gaatcagcta gtggattttt ggttaatgtc tgtaatagca acccagcaat
     4801 tcctaaagaa gctcccccaa agatacagac aataatacga ggtaaacgaa tgttagtaat
     4861 gataaaaagg aaggcatcat cagattttcc acttattagt tttaaaacgt caataacgga
     4921 agtattagca tagccaagtg aaattgatac tagtattaat aatagtagga taatgacaga
     4981 tgatattaaa acaaaaggag ctttattttt ttgaatcata gatttttccc ttttctaatt
     5041 aaccatagga aacatggaag ccctacgata ctgattatag aactaattgg tgtctctgaa
     5101 gggggattta taatacgaga tgataagtct acccagatca tgaaagtagc ccctgaaaat
     5161 cctattaaag gtaataacag acgataatct ttgggaataa aaagcttaat aaaatgtgga
     5221 ataatcaatc caatgaaaga aacggttcct atcaacgcga ctgatgaagc cgataaaaac
     5281 aaaacaataa gtaatagaaa cgcagtcatt aattgagttt tttgtcccaa tgctttggcc
     5341 acagattcat taaggctcaa gatagttaat tgatgtgcta ggagttgtga aataagtaaa
     5401 cctaaaataa tgataggtgc tattatgatt agcattttcc agttaatttg ggataaacca
     5461 cctgcttgcc aaccgataac agttcgattg agtttaaagt ataatgtaac cacttgtcca
     5521 acagacgtaa aaagtgtgga gatcatcgct ccagctaaaa tgagtcgtag ttggtggtaa
     5581 cccttttgct ttgtataaga gagtgtgaag actaaaagac aagaaatgac agaccctagt
     5641 aagcagacaa tgagaatcgt actatagtgc atgctcccta aaaaagcata tgcaacaaca
     5701 agggctaacc cagctccggc gttaatacct agtagtccag gatcagcaat ggcgtttcga
     5761 gtgactccct gcataatagc acctgcttgt gacatagctg cacctactaa gatagctgca
     5821 attatgcgtg gtagtctaat gtcaaaaata acatcctgag cattagagtt ttttaaggga
     5881 ttttttaaaa cagttattaa atcactagtc ttgaaattga tagcaccaaa tcgtaagctg
     5941 gcgtaacaac caataagaaa tagtataagt gttatgaaaa aaacaagcca aaaatgattt
     6001 gacttatttt tttgaacgag tttttttgaa gctttaaaca taagaggagc tttcttttaa
     6061 tttgtatttt ctttgatagc ctttgtaaat gattttaatt gagcttctaa agatagaggg
     6121 tcagagaaat aaaacacgtc gtagttactt tctatgatgt gccctttttt gacagctggt
     6181 aaattcttcc agacatcact ttctttaagt gatgaagctg cttttttagt cgttttgttt
     6241 atattaacaa gggcataatc tccaacgtaa tcaccgattg cttcttgcga aacggtaaac
     6301 cacccttttt taaagacatc ttttttgact ttttctgggg cagcataacc tagtgaatca
     6361 tagattagtt ctccaccgcg tccaaaatta ttaccatata aatagatatt tttatcataa
     6421 aaatccataa tagtaaaagt agtgttaggc tttaagatat ggtgtaaatc ttttttggca
     6481 gcgagagttt tagttttcca ttggctaacc cactgattag cttctttttc tttaccgaat
     6541 actttcccca aggctggcat catatctaaa taattttgtg caccatattt aataactaaa
     6601 gttggtgcaa tttttttcag agtattgatg tttggatctt gatcgaaaac catgattaaa
     6661 tcaggttttt gtgcggcaat agcttctgta tcatcagcag ttaatttttt agcttctttc
     6721 agttgcttac caaaaacggg gctatctttt tctaagtcta aactgtaact tgaaacatta
     6781 acacctagtt ttaataaata cccagtgtaa gaatatgtaa aattaattac ttttttcgga
     6841 ttttcaggaa tttttccata ataggtgaag ccttcaattt taggcatttt agaaatagtt
     6901 gttttagtgc tttcttcttt agtttgttgt ctgcaagata ccaaaaagaa ggtcagtagt
     6961 gtgaggacaa taattccaat ttttttcata agataacatc tcctaaataa tattaatttt
     7021 gatcagttga taattgataa gataacaaaa caggttgttt actaataggg tcctgaatga
     7081 tatgagcatc aatcttaaaa atatctttga taatatcttt agtcatgatc tgactaggac
     7141 taccttcata gataatttta ccggttttca ttgcaactag ataatcagaa tagcgcgcag
     7201 acaggtttag gtcgtgtagg accataataa ttgttttttg cgtttcgtta ttaagttttt
     7261 tgagcaattc taagacttct aattgatgat tcatgtcaag ataagttgta ggttcgtcta
     7321 aaaagatagt atctgtatct tgtgctaaag ccatagcaat ccatactttt tgcctctgac
     7381 ctcctgaaag gtcatcaaca tctctattag caaattgcgc tacgttagtc atttccatcg
     7441 cccaatggat tttactacga tcatcatttg tcaaattgcc aaggtatttt tggtgtggga
     7501 accgtccata agatattagt tcataaactg ttatcccttc agttgcttct aacacctgtg
     7561 ggagaagtgc aatcttctta gcaatttcct ttgtgggtaa atgtgcaata tcatgaccat
     7621 caatagtgat agtaccatct tttattttat ggatccgtgt gagagccttc aaaagagtag
     7681 actttccaca tccattggca ccaataatcg ttgttatttt ttggttaagt atactgaggg
     7741 aaagattatt gatgatttct ttctgatcgt aagatacaat aatgttttct gcttttatgt
     7801 gagacataga gaacctttct agcaaaaaat taagttcatt ataacataga aaaaaggctt
     7861 acaaaagcct tttttagcat taattaagaa aaatgtgaac gaaatgataa ttatttagaa
     7921 tgattcaaaa tagtatcaat aatcattatt gtttagcttt ttttatcatt taaggtattt
     7981 tttcgcaact tctaaatctc cagagtaatt gtcacgattt cctttgataa tttgataagc
     8041 atctgcccct ttaccagcga taataattgc atctagtttg caattagtaa gagtactggc
     8101 attagcaatt gcttcttctc gatcaatgat gatagtaacc ggacgattaa tatggcttgc
     8161 gatttcttgt gagataacta agggatcttc aaaattagga tcatctgcag tgagtatcac
     8221 ttgtagatta ggatgttgat taatgacatc tccgaagtct ttacgacggc tttgaccttt
     8281 attaccagga gctccgagca ctaatataat atcccctttt tggtgttcct caacaacagc
     8341 taataatttc ttcaaactgt ctccgttgtg ggcatagtca acgaaaatct ttgcgccgtt
     8401 ggtttgtgtt aggacttcca tgcggccagg gacggtagtt tgtgcaattc cgttttttat
     8461 atcttcaatt gaaacaccaa gtcggagaca agcaagtcca gctgcgagag cattttcttg
     8521 attgaatttt ccaatcaggt tgatattgta ggtgttctcc aactttcctt tgacatggaa
     8581 agaaaaggcc tgtgattctg tgataacatt ttcagaataa tctccataga aatcatgagg
     8641 aatatattct acctgttctt tgacaatatt aaaatgatcc atctgactat ttacaacgac
     8701 agcattgcta ttctccatca agagccgttt gtggaaaaaa taatcttcga atgtagggtg
     8761 ctcgatagga ccaatatgat cgggactaat atttaagaaa acgccaacgt caaaggttaa
     8821 accataaaca cgtttggtta ggtatgcttg gcttgatact tccatgacta agtgtgtcat
     8881 ttgattctca acggctgtag ccatcatccg aaataaatca agactctcgg gggttgtgag
     8941 atgtgattta aagaaagatt taccatctaa tgttgtgttc atagttgaga gcatagcagg
     9001 acgatggttt acttttagca tatgataagc aaagtaagcc gcagttgttt ttccttttgt
     9061 tccagtaaaa gctagaagtt taagtttgtt ttgtgggtta ttatagaaag acatagatat
     9121 caaactcatg gcttttttga tatcattgac taatatcact ggaatatcgg cgccgtaatc
     9181 tatctcagaa acgtaaaaag aaaggcctgc tgtgattgca gaatctagat attctttttt
     9241 aaaagtggca cccttagcaa aaaataaagt atcagatgaa atttgacgac tatcatatga
     9301 taaggcatta aaagttacat tttgtgtcca gttataataa taatgctcat gaaacaaaat
     9361 ctctctgaaa ttatggtcat tttttaaaat ttctaaaatt ttatctattg taatcatact
     9421 ccttatttta gac
//
LOCUS       DATRQA010000072         9203 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.072, whole genome shotgun sequence.
ACCESSION   DATRQA010000072 DATRQA010000000
VERSION     DATRQA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.072"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(96..1721)
                     /gene="treC"
                     /locus_tag="VBD27_001300"
     CDS             complement(96..1721)
                     /gene="treC"
                     /locus_tag="VBD27_001300"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEO7025309.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLAELGID
                     MVWLNPFYPSPQRDNGYDISDYTAINPDFGTMDDFEEMIEVGRQYRIDFMLDMVLNHC
                     SIEHEWFKKALAGDRYYQDFFILRDNPTDWVSKFGGNAWSPFGDTGKYYLHLFDITQA
                     DLNWRNADVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEILENCPINDGKPAYTDRP
                     ITHDYLKMLNNASFGQADSFMTVGEMSSTTIANCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMAFDFPALRDLFHSWGEGMSEGNGWNALFYNNHDQPRALNRFVDVKRFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDYSSMDDYVDIESLNAYQIMLDEGKSQE
                     EAFSIIRAKSRDNSRVPMQWDDSTNAGFSEGAPWLKVGKSYKEINVAKEKTGLIFTFY
                     QELIRLRKQLPIIADGNYKAAFKDNEKVYAFERHLDKEKLLVLNNFFAEKVKIKLPEN
                     YLQGQVLLSNYKDVTLDETVTLQPYQTLAILVS"
     gene            complement(1943..3973)
                     /gene="treP"
                     /locus_tag="VBD27_001301"
     CDS             complement(1943..3973)
                     /gene="treP"
                     /locus_tag="VBD27_001301"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEO7025310.1"
                     /translation="MEQFKHDAKALLEAIGGKENISAVTHCATRMRFVLNDSSKAKVK
                     VIEELPSVKGTFTNAGQFQVIIGNDVPIFYNAFVAVSGIEGVSKEAAKSAAPKNQNPL
                     QRVLTMLAEIFTPIIPAIIVGGLILGFRNILDAVPFEFLGQKVVDGVRQVDSSGHPIW
                     NTLVDVSTFWSGVDSFLWLPGEAIFHFLPVGIVWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYSVASTSAADIAKNWSWNFGYFTVQKIGYQAQVIPALLAGLSLSYLEIFWRKHI
                     PEVVSMIFVPFLSLVPAIILAHTVLGPIGWTLGKWISAIVLIGLTGPVKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADTKTHTTGLWPMIALSNIAQGSAVLAYYFMHRHDE
                     KEAQISLPAAISAYLGVTEPALFGVNVKYIYPFVAGMIGSSVAGLLATTFNVQANSIG
                     VGGLPGFLSINVKYMGYFFICMAVAIFIPLFLTLFFKKSGILTKTEEEKLVSDAVIAS
                     TTETKSAKEKAVVSGTKLSVVSPLSGLAKPLDQASDPVFSQGIMGKGVVIDPSDGELV
                     SPVDATVSVLFPTKHAIGLLTSEGVEFLIHIGMDTVNLEGKGFTSHVAQGDNVKVGDK
                     LITFDIPMIKEEGYIVETPILITNQEEFRPEELIDLPKQIKRGQALMVAKKI"
     gene            complement(4255..4881)
                     /locus_tag="VBD27_001302"
     CDS             complement(4255..4881)
                     /locus_tag="VBD27_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025311.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEINLEVAPGQVLGIFGQSGCGKTS
                     LSKVLAGFLHPRSGEVLVDGSNLPNKAFRPVQLIQQHPEKTMNPLWPMKKSLEEAYYP
                     SRDLLDAFGIQEKWLNRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCNQCYMIEENRIVSFNGD"
     gene            complement(4865..5668)
                     /locus_tag="VBD27_001303"
     CDS             complement(4865..5668)
                     /locus_tag="VBD27_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025312.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEIKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNASVTGDMIYRGQSLNSKRIKQLRGKDITLIPQSVN
                     YLDPSMKVKHQVRLGISENSKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDKVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGNGEQLQTEFARSLWRSLPQQEFLKGVTHDLRG"
     gene            complement(5680..6501)
                     /locus_tag="VBD27_001304"
     CDS             complement(5680..6501)
                     /locus_tag="VBD27_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025313.1"
                     /translation="MILRRRTIVLWQLGIAISLILSILALNLYFHSTPLQTNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVILATVFGVLAGLGNSIID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYHLKN
                     KEFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(6498..7475)
                     /locus_tag="VBD27_001305"
     CDS             complement(6498..7475)
                     /locus_tag="VBD27_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025314.1"
                     /translation="MKHITTVVIWKILRCLSLVFGVSILTFILLKQSPVDPVMASVNY
                     DTSLTPVQYKAIAHHYGLDKPAPVQYFIWLKNFIQGHLGTSLVYRQPVIDIIRSRAGA
                     SFILMGLSWVLSGLIGFILGTLSAFHQGKLLDRIVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLILPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETEWQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(7588..9078)
                     /locus_tag="VBD27_001306"
     CDS             complement(7588..9078)
                     /locus_tag="VBD27_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7025315.1"
                     /translation="MGAKLPHEFDPKDRYGIHNEGNITHSTLLKRSPELDIKGELAKK
                     YKISKDGLTWSFDLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGK
                     NQVNIHLTEAHSTFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVR
                     NPYWHGKKPYFKKWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIASN
                     DVRGLSLPYVKKGVVKNSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKP
                     AYSIIDRTPFWNPKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTN
                     DQLRANLAVEVAEQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHY
                     PSLAGKGWTNITFYNNPTVTKYLDKAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPN
                     VWLVSLNHTYIGDKRINVGKQGVHSHGHDWSLLTNIAEWTWDESAK"
BASE COUNT         3009 a         1864 c         1529 g         2801 t
ORIGIN      
        1 aagaaagaaa ttgcaaaatc tagagcaaga ttccttccca caagcagcac ttaaaaaacc
       61 tttacttaat tgaataagta aaggtttttt aaatgttatg acactaagat agctaaagtt
      121 tgataaggtt gcaaagtaac cgtttcatca agagtaacat ccttataatt tgatagaaga
      181 acttgtcctt ggagataatt ctccggtaat tttatcttaa ctttctcagc aaagaaatta
      241 tttaaaacaa gaagtttttc cttatcaaga tggcgttcaa aagcatatac tttttcgtta
      301 tctttgaaag ctgctttata attcccgtca gctattattg gcagttgttt acgtagcctt
      361 atcaactctt gatagaaagt aaatatcagc cctgttttct cctttgcgac attgatttct
      421 ttatatgatt ttcctacttt caaccatgga gcgccttcag aaaaacctgc attcgtacta
      481 tcatcccatt gcattggtac tcgagaattg tctcgagatt tcgctcgaat aatgctaaat
      541 gcctcttctt gtgacttgcc ttcatcaagc attatttggt aggcattcaa actctcaata
      601 tccacataat catccataga actgtaatcg ggatcaagca ttccgatttc ttcacccata
      661 tatatataag gagtaccacg cgataagtgg atagatgctg ctaacatggt tgctccttca
      721 tttcgaaaac gtttcacgtc tacaaaacga tttaatgctc gcggttggtc atgattatta
      781 tagaataaag cattccaacc atttccttcg ctcattcctt cgccccaact atggaaaagg
      841 tcacgtaatg caggaaaatc aaaagccata atggtccact tttgcccatc tttataatct
      901 actttgaggt ggtgaaaatt aaaagccatg gataactctt cacgttctgg tgcagtgtaa
      961 agaatgcaat ttgcaattgt cgttgaagac atttcaccaa ctgtcatgaa ggaatcagct
     1021 tgtccaaaac tagcattatt caacatcttg agataatcat gtgttattgg acgatcagta
     1081 taagctggtt ttccatcatt aattgggcag ttctcaagta tctcatcttt cccaattaaa
     1141 ttaatcacat caaatcgaaa acctttaacc cctttatcac gccaaaaatt gacaacttta
     1201 aaaagttcct tgcgaacatc ggcattgcgc caattaaggt cagcttgagt gatatcaaaa
     1261 agatgaagat aatacttccc tgtatcacca aatggtgacc aagcatttcc accaaattta
     1321 gatacccaat ctgttggatt atcacgtaag ataaagaaat cttgatagta gcgatctcct
     1381 gcaagtgctt ttttaaacca ctcatgttct attgaacaat gattaagcac catatcaagc
     1441 ataaaatcta tacggtattg acgtcccacc tcaatcattt cttcaaaatc gtccatcgtt
     1501 ccaaaatcag ggttaatggc tgtataatca gagatatcat atccattatc tcgttgtgga
     1561 cttggataaa atgggttgag ccaaaccata tcaataccaa gttctgctaa atagggtaat
     1621 ttttcgataa taccacgaag atcaccaacc ccattaccag ttgtgtcttt gtatgatttt
     1681 ggataaattt gatagacaac tttacgttta tcaatcgtca tattctgctc ctttcccaaa
     1741 atgctgctgt atttagcagt ctaactataa catcaactat catcaaaaat ataacatttt
     1801 tacagcgata tttgttccca tgagtttttt cttatgttca aaataattca aggagctaat
     1861 gtattaagcc cctcaaacaa ttcctcaaat tacctctcat tttataagag tgctcttttt
     1921 taaaaaggat acctaattac ttttaaattt tcttggcaac cattaatgct tgtccacgtt
     1981 ttatctgttt gggtaaatct atcaattctt ctggtcggaa ttcttcttga ttagtaatca
     2041 gaataggagt ttccacaatg tatccttctt cttttatcat tggaatatcg aaagtaatga
     2101 gtttatctcc tacttttaca ttgtcacctt gggcaacatg agaggtaaac ccttttcctt
     2161 ctaaattaac agtatccata ccaatatgaa taagaaactc aacaccctct gatgttaata
     2221 atccaatagc atgtttcgta gggaaaagaa ctgaaactgt cgcatcgacc ggtgaaacta
     2281 gttctccatc acttggatcg ataaccactc ccttccccat aattccttgt gaaaagacag
     2341 gatcactagc ttgatccagt ggtttagcta aacctgataa agggcttact acactaagct
     2401 ttgtacctga gacgacagct ttttcttttg ctgattttgt ttctgttgtt gaagcaatga
     2461 cagcatcaga tactaatttt tcctcttcag tttttgtcaa aatacctgat tttttaaaga
     2521 atagtgtcaa aaataaagga ataaagatag ccactgccat acagataaag aaatatccca
     2581 tatatttaac attaattgac aggaagcctg gaagaccgcc tacaccaata gagttagctt
     2641 ggacattaaa agttgtcgcc aaaagacctg ccacacttga accaatcata ccagcaacaa
     2701 atggataaat gtatttaacg tttacaccaa aaagcgccgg ctctgtaaca ccaaggtagg
     2761 ctgagatagc agctggaagt gatatttgag cctctttttc atcatgacga tgcataaaat
     2821 aataagctag tacagctgaa ccctgagcaa tatttgaaag agcaatcatc ggccaaaggc
     2881 cagttgtatg tgtttttgta tctgctatga gttgagtatc gatagcattt gtcatgtggt
     2941 gcaaaccagt aataacaaat ggggcataca gggcaccaaa aatagcacca aagagccatt
     3001 ttacaggacc agtcaatcca ataagaacga tagcagaaat ccatttacct agtgtccaac
     3061 caataggtcc aaggacagta tgagccaaga taatagctgg aactaaggat agaaatggca
     3121 caaaaatcat tgaaacaact tcaggaatat gttttctcca gaagatttct aagtaagaca
     3181 ggctcaaacc agctaacaga gctggaataa cttgagcttg ataaccaatt ttttgtactg
     3241 taaagtaacc aaaattccaa gaccaatttt tagcaatgtc tgctgctgaa gtactagcta
     3301 cagaataagc attaagtaat tgtggcgaca ctagacagat acctagaaca ataccgagga
     3361 tttgagtagt ccccatctta cgagtcacag accatacaat accaactggt aagaaatgaa
     3421 atatagcttc acctggtaac cataaaaaag agtctactcc gctccaaaac gttgaaacat
     3481 ctactagagt attccaaatt ggatgcccag aagagtctac ttgcctgaca ccatcaacaa
     3541 ctttttgacc taaaaattca aatggaacgg catctaaaat attacgaaaa ccaaggatca
     3601 gaccaccaac aataatggct ggaataatcg gtgtaaatat ttcagccagc attgtcaaca
     3661 cacgctgtaa agggttttga ttttttgggg cagccgattt tgccgcctct ttagacacac
     3721 cctcaatacc agaaacggct acaaaagcat tataaaaaat agggacatca ttaccaataa
     3781 tgacttgaaa ttgcccagca ttggtaaagg tacccttgac tgatggtaac tcctcaatta
     3841 cctttacctt agctttacta ctgtcattca aaacaaatct catgcgtgtg gcacaatgtg
     3901 tcactgcact aatattttct ttacccccaa ttgcctctaa caaggctttg gcatcatgtt
     3961 taaattgttc catctgttta tccttatcta tttaacggag agtctgtgac tccaaggcag
     4021 aaacttaaag aagactagct tacaatataa ttgtaaacga ttacatatcc ctatgcaaat
     4081 ccttagctta tctttttaaa aaatctataa attataaaat aattagtcaa taatacccaa
     4141 aacattagaa tttctcacca acgttaccaa aatgttagag tcatgagtag gtcagtcacc
     4201 cccaatactc tcttaataaa aaacacctac taattgatat agtaggtgag ttttttaatc
     4261 tccattgaaa gatacgatac gattctcttc aatcatatag cattggttac aaagtttctc
     4321 taacatagcg aaatcatgac taataacgat gagacctaaa tttctatcct ttacaatctc
     4381 caacaggctt ttccatacac tagcttgtgt aatactatcc aacatagtag tcatttcatc
     4441 tgcaataagg tatttagtct ctggatgtaa tgaacgcaca atcgaaaagc gttgtaattc
     4501 ccctcccgag agttcactag ggcgacgatt tagccatttt tcttgaattc caaaagcatc
     4561 tagtaaatct cgacttgggt aataagcttc ttctaaactt tttttcatag gccataaagg
     4621 attcatcgtt ttttcaggat gttgttgaat aagttgtaca ggtctaaatg ctttgttagg
     4681 caaattactg ccatcaacta atacttcacc agatctagga tgtaaaaaac ctgcaagcac
     4741 tttggataaa ctagtttttc cacaaccact ttgtccaaat atacctaaaa cttgaccagg
     4801 tgctacctct aaattaatct ccttaaaaag ccattgatct tttttatgat aaaagccaag
     4861 ctttttagcc tctaaggtca tgagtaactc ctttcaaaaa ttcttgctgt gggagagagc
     4921 gccataaact tctagcaaat tctgtttgta actgctctcc atttccgcta aagaaactag
     4981 ctggagctgt ttcaatagct tttccctctt taaaaatagt aatacgatca gcaatttgac
     5041 tagctgctac aatatcatga gtgataaata taacgcttat tcctttatct gcaaaggagc
     5101 gtagttggtc taaaaccatt tgcagagcat ctggatgtaa tccaggggtg ggctcatccg
     5161 caataatcaa agaaacctta tcactaatac acgttgtaaa caaaacacgt cggagcattc
     5221 cgccagaaag ttggaaagga tacaagtcac catcactttc ttttaaacca aactgttgaa
     5281 acaatccttc ttgagtagcc tttgaatttt ctgagatacc taagcgcacc tgatgtttga
     5341 ctttcataga tggatctaaa taattaactg attgtggaat caacgtaata tcttttcctc
     5401 gcaactgttt aatgcgttta gaatttagtg attgaccacg ataaatcata tctcctgtta
     5461 cagatgcatt tttaggaaga atatccataa tagcatgtgc taataaactc ttccccgaac
     5521 cactagcacc tataatagct aataactcac cttttttaat ttctaaattc agcgcttgta
     5581 tcggtgttga ttgaaatggt tttaaaaatc ttccgtattg agtgaaggtg atggagaggt
     5641 ctttaatgct taataatgtt tctgtcatat ctctacctcc taaaaatgat cagtttgagg
     5701 gtaaaagagt ttctttaaag attctccgat agtatcaaat gcattgacaa ccaaaataag
     5761 ataaagtcct ggaaagataa ccaaccacca atttccaaga gagatatgct tagctgcctc
     5821 tgacagaatg ataccaaccg aaggttgttc ggcagagagc ccaaatccta agaaagtcat
     5881 tgatgcttca tgtaggatga catgtggaaa taataggata aaaccaatga aaatttgaga
     5941 agcaatcaaa ggcaggatat gatgcctcac aatataataa ggcgtttttc ccatactttt
     6001 agaaagttgg acaaattctt tattctttag atgatagact tcattgcgga taagccttgc
     6061 taaagaaggc caatgtgtaa cagccgttgc aatgatgacc ccttgagcac ctttcccaac
     6121 aacaaaagaa atgagaatca taaaaatcaa atgaggcata ccaataaaca aatcaactaa
     6181 ccatgctatt attttatcaa taatgctatt tcctaaacct gcaagcactc caaaaactgt
     6241 cgccagaatg acccccataa gggcacctaa taagccgact tgtagagaga aataaagtcc
     6301 tttaatcgtt ctgacaaaca tatccctacc taaaccatct gtcccaaaaa gatggtttaa
     6361 tgaaggagca aggttccgta aagctgcatt ggtttgcaag ggagtactat ggaaataaag
     6421 atttaaggct agaatactaa gaatgagaga aatggcgata cccagttgcc ataaaacaat
     6481 agttcgacgt ctcaatatca tacttttctc cttaactgtg gattgattat gctatttaaa
     6541 atatccgcaa taagattgcc cgcaaaaaca aataatgtcc ctatcatcac aatagctaga
     6601 agtagcggtg tatcactttt aagtcctgct tcagttaggg tagaccctag tcctggatat
     6661 gagaaaactt gctcagcaag aacggatcct ccaaacaatt ctccaaaata ggaaaaatgc
     6721 agtgtaatag ctggtacgat agcatttcta agacaatgat ttttaaaaat ttgccattcc
     6781 gtttccccac gcgctctggc aaataagaca tattcactag aaagtaccga catcatttta
     6841 gttctagtat gaagagttac attggcaatg cctagaatac ttagcgtgaa aacaggtaaa
     6901 ataaggtgct taatacgatc agctaacgta atatcttgac tcaaagtacc tatcggagaa
     6961 gaaataccaa tcgggaacca ccccagctgg acagaaaaga ttaataaaaa aatgagccca
     7021 atccaaaaag ttggtactga tatctgaagg taagaaaacc accttacaat tcgatcaagc
     7081 aatttccctt gatggaaagc tgataaagtt cctaagataa atccaataag acccgataag
     7141 acccaggaga gtcccataag tatgaaagag gcacctgccc gtgatctaat aatatcaata
     7201 acaggttgcc gataaactag tgaagtccct aaatgtccct gtataaaatt ttttaaccaa
     7261 ataaaatact ggaccggagc tggcttatcc aagccatagt ggtgagcaat cgctttgtac
     7321 tgaacagggg ttagtgacgt gtcataattg acacttgcca tgactggatc aaccggtgat
     7381 tgttttagta aaataaaggt caaaattgat actccaaaaa cgagtgacaa acacctaagt
     7441 atcttccaga taactacagt ggtaatatgt ttcacagata aatctcctta attgttaaaa
     7501 aaggagtcga ggaaaccatt cacaccataa ggtgtacatt gttttctctg actccaaact
     7561 taaagtagcc tacattaact aaattagtta cttagcagat tcatcccaag tccattcagc
     7621 aatgttagtc aataatgacc aatcatgacc atgactatgg acaccttgtt tacctacatt
     7681 gatacgttta tcaccaatat aagtatggtt aagactcacc aaccatacat ttggtaaatc
     7741 tccaagagtg gaagcacctg ttttgccatc ccactgagct aacttccaat atttgttagc
     7801 tttatcaagg tcgggagatg tcattgcttt gtcaaggtac ttagtcacag taggattgtt
     7861 ataaaaagta atattggtcc aacctttacc agctaaactt gggtaatgtg attcataaaa
     7921 ttgctgcgca tgatgacgtc ctcccgcata aagtaaggct gaatcatgtg acttagttgc
     7981 catttcatcc cagttactag ccttgagttt aatagtaata cctaaggctt tagcttgctc
     8041 cgctacttca acggctaagt ttgctcgtaa ttgatcatta gtagggtagt aaaggtcaaa
     8101 ttccgcttta agatttcctt ttttgcggct accgtctgct tgttctttcc atccagcttt
     8161 tgtcaaaagt tgcttagctt tagctacttt attatcttta attgctgttt ttggattcca
     8221 gaatggtgtt ctatcaataa ttgaataagc tggtttacca taaccattta aaacagtatc
     8281 cagaactttt tgtctattta aaccaatagt caaggctttt ctgattgctg gatcactagt
     8341 gacatcattt cctactggat aaccgtctgg tgaatttttc acaacacctt tcttcacata
     8401 aggtagcgat aacccacgaa catcatttga agcaatatct agaagacgtg ttcctttgac
     8461 tttcttgcta gcaagctctg gcgttgcgta aatcatgtca acatcaccag attctaaagc
     8521 agctagtgct gtattttcat caagtaatac ccaagtccac tttttaaaat aaggcttttt
     8581 accgtgccaa tatggatttc gaacgaagat tgcttgttct ccagccttat attcttttac
     8641 catgtaaggt cctgaaccga taggattgct cttatactta tcattgtaat gttttttagg
     8701 gacgattggg atttctgtca actgtgctgt aaatgtcgaa tgcgcctcag tcaaatgaat
     8761 atttacctgg tttttcccaa ctacttcaac attcttaata aaggtcaaat cccaagcttt
     8821 tccatctgct ttcaacatat cataagtaaa cttaacatcg tcagcagtaa caggctcacc
     8881 attggagaat ttaaaatcat catttaagtc gaacgaccac gttaagccat ccttagagat
     8941 tttatatttt ttagcaagct ctccttttat atctagttca ggagaacgtt tcaatagggt
     9001 actatgagta atattacctt cattatggat tccataacgg tcctttggat cgaattcatg
     9061 aggaagcttt gcccccatag agacaaccaa ttcatctttg ggtcgttgtt ttcgtgttcg
     9121 ctcctttgtt tgtgaatttt gattttgaca agctactaaa aagatactcg ccagaaacaa
     9181 agcaacaaaa gagatgtact tca
//
LOCUS       DATRQA010000073         9173 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.073, whole genome shotgun sequence.
ACCESSION   DATRQA010000073 DATRQA010000000
VERSION     DATRQA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9173
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.073"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..782)
                     /locus_tag="VBD27_001307"
     CDS             complement(<1..782)
                     /locus_tag="VBD27_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEO7025316.1"
                     /translation="MTYTSLKSPTTKDYKYTLNVAHLYGNLLNTYGDNGNILMMKYVG
                     EKLGCQMTFDIVSLEDRFDPNYYQMAFFGGGQDYEQAIVARDLPSKKEDINKFIQNNG
                     VVLAICGGFQLLGQYYIQANGERIEGIGVMGHYTLNQNNNRYIGDIKIHNDEFNETYY
                     GFENHQGRTFLSEDEKPLGTVIYGNGNNKEDGTEGVHYKNVFGSYFHGPILSRNANLA
                     YRLVATALRNKYGKEIVLPSYEEILSLEIPEEYGDVKSKADF"
     gene            complement(782..2125)
                     /locus_tag="VBD27_001308"
     CDS             complement(782..2125)
                     /locus_tag="VBD27_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEO7025317.1"
                     /translation="MKINTALGVAAGKSAHYLLSKMGRGSTYPGSLALKFDKDILDTI
                     AKDYEIVVVTGTNGKTLTTALTVGILKEAFGQVVTNPSGANMITGIVSTFLTAKKSKS
                     GKKIAVLEIDEASLPRITQYIKPSLFVFTNIFRDQMDRYGEIYTTYQMILDGAANAPQ
                     ATILANGDSPLFNSKSVTNPVQFYGFDTDKHEPRLAHYNTEGILCPKCQAILTYRLNT
                     YANLGDYTCPNCDFERPNLDYALTRLIHLTNTSSGFVIDGQQYNINVGGLYNIYNALA
                     AVSVAEYFGVEASQIKDGFDKSRAVFGRQETFTIGNKKCTLVLIKNPVGASQALDMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDANFETILTMNIPEIFAGGVRHSEIARRLRVT
                     GYDEKRIKQADKLQDIMAMIEQQETEHAYILATYTAMLEFREILANHNAIRKEMN"
     gene            2265..3116
                     /locus_tag="VBD27_001309"
     CDS             2265..3116
                     /locus_tag="VBD27_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEO7025318.1"
                     /translation="MDIFSAIDSKFWASIMENPWMILIHLMDIIIVAVLIYKFIKALA
                     GTKIMSLIQGVILFIIIRFVSEWIGLTTITFLMNQIVTYGVIAGVVIFAPEIRTGLEK
                     FGRTPQLFTQRSQLSSDEKLVDALVKAVAYMSPRKIGALISIERTQTLQEYIATGIPL
                     DADISSELLINIFIPNTPLHDGAVIVKDKKIATACSYLPLSESSSISKEFGTRHRAAI
                     GLSENSDALTVIVSEETGGISVALKGEFLHDLSKDSFEAILRTQLIQNQEENSKLAWY
                     NQLLRRK"
     gene            3119..4078
                     /locus_tag="VBD27_001310"
     CDS             3119..4078
                     /locus_tag="VBD27_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEO7025319.1"
                     /translation="MKKFFTNKFWLGVVSLFLAILLFLTATATSMNHQDNSKIAGASE
                     TYTHTLTDVPIDIKYDSDDYFISGYSYGADVYMSSVNRVKLDSEINEDTRKFKVVADL
                     TNMKPGTHKVPLKVVNLPSGVNATVSPTTITVTMGKKKTKEFPVYGHVNDKQIKAGYA
                     VDKMSVDVSKVKVTSDESIIDRIDHVAANIPDDKVLDDDFNKTVTLQAVTADGTVLAS
                     IIHPSKATLSVKVKKLTKTVPINLIPVGQFSDSISKINYKLSQEKAVISGTKEALEAI
                     SVINAEVDISDVTKNTEKKINLSANNVSVDPAQVTVQLTTTKK"
     gene            4132..5484
                     /locus_tag="VBD27_001311"
     CDS             4132..5484
                     /locus_tag="VBD27_001311"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO7025320.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGSDGFKLDDDRELEIEALLDAKEDTLPRPSAQGLGTLVDYPEGLRKY
                     EKFMESTGIDLEGMNVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGS
                     THPEQLQSLVRENGSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLA
                     QNTIVTTVMSNLGFHKALDREGIHKAITAVGDRYVVEEMRKSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLTKVMKETGKKLSELASEVTVYPQKLVNIRVENNMKDKAMEVPAI
                     AEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD
                     "
     gene            5607..5978
                     /locus_tag="VBD27_001312"
     CDS             5607..5978
                     /locus_tag="VBD27_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEO7025321.1"
                     /translation="MTKEAELLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSL
                     IDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFL
                     KQLLAKYPNSYLQNSSIFEEE"
     gene            6003..6383
                     /locus_tag="VBD27_001313"
     CDS             6003..6383
                     /locus_tag="VBD27_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025322.1"
                     /translation="MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFR
                     YSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHN
                     QDYLQECLWNLKAKGIDLPINKIK"
     gene            6476..7606
                     /locus_tag="VBD27_001314"
     CDS             6476..7606
                     /locus_tag="VBD27_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEO7025323.1"
                     /translation="MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQALIRE
                     FRSYDITELRTLYIGGGTPTSISAVQLDYLLTELSRDLNLNTLEEFTIEANPGDLTVD
                     KIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEVQIYSTIDALKTAGFQNISIDLIY
                     ALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEM
                     FEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWDNVEYYGVGAGASGYLDGIRYRN
                     RGPIQHYLKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKKGVSIGKFEQKFGTSFEK
                     RYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE"
     gene            7610..8347
                     /locus_tag="VBD27_001315"
     CDS             7610..8347
                     /locus_tag="VBD27_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO7025324.1"
                     /translation="MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGI
                     GDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQ
                     KIITISSAFVLMDFKTRKIHPVLDDITSIYQSQRIKKVIRGPKYHPIGDSKVKQYHVR
                     YFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYIKEIQYGTDIKSHWYQDG
                     LVTRHDIIGGDAIHAQARIEWQEKKED"
     gene            8348..9118
                     /locus_tag="VBD27_001316"
     CDS             8348..9118
                     /locus_tag="VBD27_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEO7025325.1"
                     /translation="MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNN
                     TTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIAD
                     AGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGA
                     GSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINND
                     IDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG"
     gene            9108..>9173
                     /locus_tag="VBD27_001317"
     CDS             9108..>9173
                     /locus_tag="VBD27_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO7025326.1"
                     /translation="MKDKLLVVLTWIWIISLATLAT"
BASE COUNT         3027 a         1427 c         1820 g         2899 t
ORIGIN      
        1 tcaaaatctg cttttgattt aacgtctccg tattcctcag gaatctctaa agataaaatt
       61 tcttcataac tagggaggac aatctcttta ccatatttgt tacggagggc agttgccaca
      121 agacggtaag ctagattagc attacgtgaa aggattggac cgtgaaagta tgacccaaaa
      181 acattcttat aatgaacccc ttcagtacca tcttctttgt tattcccatt tccataaatg
      241 acagttccta aaggtttttc gtcttctgat aaaaaggtac gtccttgatg attctcaaag
      301 ccataatatg tttcattgaa ttcatcgttg tgaatcttga tatcaccaat atagcgatta
      361 ttattttggt ttagcgtata gtgtcccata acgccaatgc cctctattcg ttctccatta
      421 gcttgaatat agtattgtcc taaaagttgg aaacctccac aaatagcaag tactacacca
      481 ttattttgaa tgaatttgtt aatgtcttct ttcttactgg gaagatcgcg tgcaacaata
      541 gcttgttcgt agtcttgtcc accaccaaaa aatgccatct gataatagtt gggatcaaat
      601 ctatcttcta atgaaacgat atcaaatgtc atttgacatc ctagtttttc tcccacatat
      661 ttcatcatta agatattccc attatcgcca taagtattta ataggttacc ataaagatga
      721 gctacattaa gagtgtattt ataatctttg gtagtaggag attttagtga ggtataagtc
      781 attaattcat ctcctttctg atagcgttgt ggttagcaag aatttcacga aactccagca
      841 tagctgtata tgttgctaaa atataagcat gctcagtctc ttgctgctca atcattgcca
      901 taatatcttg cagtttatca gcttgtttta tgcgtttctc atcataacca gttacacgta
      961 agcgacgagc aatttcagag tggcgaacac caccagcaaa tatttctggg atattcatcg
     1021 tgagaatagt ttcaaaatta gcgtcccaaa tccaagaagt atcaataccg tcagcataat
     1081 tggcatttaa tagtactgaa agagagaacg ggtatggagc aagtttaatc atatcaagag
     1141 cttgactagc tcctactgga tttttaatca gaactaaggt acatttttta tttccaattg
     1201 taaaggtttc ttgacgacca aaaacggcac ggcttttatc aaatccatct ttaatttgac
     1261 ttgcttctac accaaaatat tcagcaacag atactgccgc caaagcattg taaatattat
     1321 aaaggccacc cacgttgata ttatattgtt ggccatcaat aacgaaacct gatgaagtat
     1381 ttgtcagatg tattagcctt gttaaggcat aatcaaggtt tggacgttca aaatcacaat
     1441 taggacaagt gtagtcacca aggttggcat atgtgttcaa tcgataagtt aaaattgctt
     1501 gacactttgg acagagaata ccctcagtat tatagtgtgc taatcgtggc tcatgtttgt
     1561 cggtatcaaa tccatagaat tgcacagggt tagtcacgct tttagaatta aaaagtggac
     1621 tgtcaccgtt agctaagata gtagcttgcg gagcgttagc agctccatct aaaatcattt
     1681 gataagttgt gtagatttcg ccataacggt ccatttgatc acggaaaata ttggtaaaga
     1741 cgaacaaact aggctttata tactgtgtaa tacggggtaa actcgcttcg tctatttcaa
     1801 ggacagctat ttttttacca gatttacttt tcttagctgt taggaaggtt gaaacaatac
     1861 ctgtaatcat atttgctcca cttgggttgg ttaccacctg accgaaggct tcctttaaaa
     1921 taccaactgt taaagctgtt gttaaggttt taccgttagt accagtcaca actacaattt
     1981 cataatcttt ggcaattgta tctagtatat ctttatcgaa tttgagtgct aaactacccg
     2041 gataagtaga accacgtccc attttactta aaaggtaatg agcagactta ccggcagcca
     2101 ctcctagtgc tgtatttatt ttcataaaga tattttatca taaaaagatg ctttcttgct
     2161 aaagaaaata ttatgaaaac atggtataat gtagacgatg gaaaataaga aatcaaggag
     2221 ttaagcaaga aactcttcga caggctacca gtagagagga agatatggat attttttcag
     2281 ctattgattc aaagttttgg gcgagcatta tggaaaatcc atggatgatc cttattcatc
     2341 tcatggatat tatcatagta gctgttctga tttataaatt tatcaaagcg ctagcaggca
     2401 caaaaattat gtccttaatt cagggcgtta ttttgtttat catcattaga tttgtgtcag
     2461 aatggatagg gctaacaacg attacatttt tgatgaatca gattgttact tatggtgtaa
     2521 ttgcaggtgt tgtcatcttt gctccagaga ttagaacagg tctcgaaaaa tttgggcgaa
     2581 caccacaact atttacgcaa cgttctcaat taagcagtga tgaaaagcta gtagatgccc
     2641 ttgttaaggc agttgcttat atgagtccgc gtaaaatagg agccttgatt tcaattgaac
     2701 gtacccaaac tttgcaagag tatatagcta caggtatccc attagatgca gatatttcca
     2761 gtgaattgtt aattaatatt ttcattccta atacaccact gcacgatggt gctgttattg
     2821 taaaagataa aaaaattgct acagcatgtt cgtacttacc tctatcagaa tcatcttcga
     2881 tttcaaaaga atttggaaca cgccacagag cagcgattgg tctatcagag aattcagatg
     2941 cactaacggt tattgtttct gaagagactg gtggtatttc tgttgctttg aaaggtgaat
     3001 ttttacatga tctttctaaa gatagctttg aagcaattct gagaactcag ttgattcaaa
     3061 atcaagagga aaactcaaaa ttagcttggt ataatcaact attgaggagg aagtagacat
     3121 gaaaaaattt tttacaaata aattttggtt aggcgttgtc tcactctttc tggctatttt
     3181 attattttta acggcaactg caacaagtat gaaccatcaa gacaatagta agattgcagg
     3241 agcgtctgag acatatacac ataccctaac agatgtgcca atagacatca aatacgatag
     3301 tgatgattac tttattagtg gctattctta tggggcagat gtctatatgt catcggttaa
     3361 tcgtgttaag ctagattctg aaataaatga ggatacacga aaatttaaag tagtcgctga
     3421 tttgacaaat atgaaaccag ggactcacaa agtcccacta aaggtagtaa atttgccatc
     3481 tggtgtaaat gctacggttt ctccaacaac aattacagta actatgggta aaaagaaaac
     3541 aaaagagttt ccggtttatg gacatgttaa tgataagcaa attaaagctg gttatgcagt
     3601 cgataagatg tcagttgatg tgtcaaaagt taaagttact agtgatgaat ctattataga
     3661 tcgtattgac catgtcgctg ccaatatccc tgatgataaa gttcttgatg atgattttaa
     3721 caagacagtt actttacaag ctgtaacagc agatggtaca gtattagcaa gtattattca
     3781 cccttctaaa gcaactttat ctgtcaaggt aaaaaaattg accaagacag taccgattaa
     3841 tttgatacca gtaggacaat tcagcgatag tatttcaaaa attaactaca agttgagtca
     3901 agaaaaagca gtaatttcag gaacaaaaga ggctttagaa gctatttctg ttattaatgc
     3961 tgaggtagat atttcagacg taactaaaaa tacagaaaag aaaattaatc tttctgcaaa
     4021 caatgtttca gttgatcctg ctcaagtaac agttcaattg acaactacta aaaaataata
     4081 gataataaaa acgaaaaaaa tggaggtagc gtttttcgct agaaaaaaat tatgggaaaa
     4141 tattttggta cagatggtgt tcgcggtgaa gctaatgttg aactgacgcc agaattggct
     4201 tttaaattag gacgttttgg aggatatgtt ctaagtcaac acgaaactga tcgtccgaga
     4261 gtttttgtag ctcgtgatac tcgtatttct ggtgaaatgc tggaatctgc tttgattgct
     4321 ggtctacttt cagtaggtat tgaggtttat aaacttggtg ttttagccac tcctggagtg
     4381 tcttaccttg ttcgtacaga aaaagctagt gcaggggtta tgatttcagc aagccacaat
     4441 ccagcacttg ataatggtat taagttcttt ggtagtgatg gttttaaact tgatgatgat
     4501 cgtgaattag aaattgaagc tttacttgat gcgaaggaag acactttacc acgtccatca
     4561 gcacaaggtt taggtacttt agtagattat cctgaaggac ttcggaaata tgagaagttt
     4621 atggaatcaa ctggtattga tttagaaggt atgaatgttg cgttggatac agcaaatggt
     4681 gcagcaacag cctcagctcg gaatatcttt ttagacttga atgctgatat aagtgttatt
     4741 ggtgatcaac cagatggttt gaatatcaat gatggtgttg gttcgacaca tcctgagcaa
     4801 ttacaaagtt tagttagaga aaatggttcc gacatcggtc tagcttttga tggtgatagt
     4861 gatcgtttga tcgctgttga tgaaaatgga gagattgtag atggtgataa aattatgttt
     4921 atcattggga aatatctctc tgataaaggg cagcttgctc agaatacaat cgtaacaacg
     4981 gttatgtcaa atctcggctt tcataaagcg ctcgaccgtg aaggaattca taaagcgatt
     5041 accgctgtag gagaccgcta cgtggttgaa gaaatgcgta aatcaggata taatcttggc
     5101 ggagagcaat caggccacgt tattatcatg gattacaata ctacagggga tggacaatta
     5161 acagccatcc aattgactaa ggtcatgaaa gaaactggta agaaattatc agagttagct
     5221 agtgaagtga cggtttatcc tcaaaaatta gtaaatattc gtgttgaaaa taacatgaaa
     5281 gataaagcga tggaagttcc tgcaattgcg gaaattattg ctaaaatgga agaagagatg
     5341 gatggcaatg gtcgtattct agtacgtcct agtggtactg agcctcttct tagagttatg
     5401 gcagaagcgc caacaaatga agcggttgat tattatgttg atactatcgc agatgtcgtt
     5461 cgtacagaaa ttggtttaga ttaaaacggc gttattctgc tataaagtcg tcaatgttat
     5521 aaaataataa agagagggaa ttggagtgga gcatcaagca tacgcctttc tctctctttt
     5581 tgatttgaga agggagttta gagaaaatga ctaaagaagc agaattatta tgggctaaac
     5641 acaagtattt agtcttaagt aaatctcaga aaatttattt ggatattaga caaactttga
     5701 aatcaccaaa ttgtaccgtt ttagacgttc agtcactcat tgaccaagca gtgcttttag
     5761 aagaatcacc atcacaagtg actaatgctt atatgcatat atggggctat tttaaaaata
     5821 aggcagagcg tcaagaaaaa gaagaatttt taaccttgct ggaaaaatac aggaaaacag
     5881 gttatcaacg aagaaaactg ttagcttttc taaaacagct attagctaaa tacccaaatt
     5941 cttatttaca aaactcttct attttcgaag aagagtaaaa gattgatagt tgaggtatat
     6001 aaatgagatt atggcatcaa gatattattg agttattgcc taggcagcaa cttttaggac
     6061 agcatagaga gtgttgtgca ttgagaggga atgggtgggg gagaaaacat gagactgttg
     6121 attatgtttt tcgatattca ccttataggt tatttgcata tcatcaatta gttatggaag
     6181 aaatgatgga gcgtgggtat cgtgtatcta aagaatggct aattgcagaa tataggggaa
     6241 tgaagtgccc aagatatgat acacttaatc ctgttgactt ggagacacct atctatcctg
     6301 agcacaatca agactatcta caagaatgtc tatggaattt aaaagcaaaa ggaattgacc
     6361 ttcctataaa taagataaaa tagagattta tctctatttt tattttggaa taaaaagcta
     6421 tattatcaag ttttagagga gtgttttttt aagggcaaag tggtaaaata gaaacatgtt
     6481 aaagaaacca acctcagcat atgtacacat tccattttgt acccaaatat gttattattg
     6541 tgatttttca aaagttttta ttaaaaatca gcctgtagac gcttatcttc aggctcttat
     6601 ccgtgaattt cggtcttatg atattacaga attgcgcacc ttatatattg gaggagggac
     6661 acctacatct ataagcgctg tgcaactgga ctatttattg acggagttaa gtagagattt
     6721 aaacttaaat actttagagg agttcactat cgaagctaat ccaggagatt taaccgttga
     6781 taaaatagag gtgcttcaaa agtcagctgt caatagagtt tctctaggag ttcagacctt
     6841 taatgataaa catttaaagc gtattggacg cagtcacaat gaggtacaaa tttattctac
     6901 aattgatgct cttaaaacag ctggttttca aaatatctct attgacctaa tttatgcctt
     6961 acctggacaa acgatggatg atgttagaag taatgtagcc aaagctctct ctctaaatat
     7021 tcctcatttg agtctatata gtttaatttt agaacaccat actgtattta tgaataaaat
     7081 gcgtcgtggg aaattacatt tgccaacaga agatttagaa gccgagatgt ttgaatatat
     7141 catttctgaa atggagagaa atggttttga gcattatgaa atttctaatt ttacaaagcc
     7201 gggttttgaa agtcgtcata atttgatgta ctgggataat gtagaatatt atggtgttgg
     7261 tgcaggtgct tctggttatc ttgatggtat tcgttatcgt aatagagggc caatacaaca
     7321 ttatctaaag ggtgtttcag aaggtaatgc aaggttatct gaagaagtat tatcaaaaaa
     7381 tgaaatgatg gaagaggagc ttttcctcgg tcttcgtaaa aaaaagggtg tttccatcgg
     7441 aaaatttgaa caaaaatttg gaacgtcctt tgagaaacgt tacggtcaga ttgttcaaga
     7501 actccaaagt gatgggcttt tgaaagaaaa taatggtttt atccagatga ctaaaaaagg
     7561 tctcttctta ggagatacgg ttgccgagaa atttattgtg gagtaaaaaa tgggtttatt
     7621 atatcgagaa acttacgaag tgccttttta tgaatcagac actaatcatt atatgaaatt
     7681 accgcagttg ttggctttag cgttacaaat ttcagccaaa caatctttaa aattaggaat
     7741 aggcgatgac atcgttttta aaagatacgg attggtttgg gtcgttacag attatattat
     7801 tgatattgag cgtttaccca agcatgctga aaagatagtc attgagactg aagccaaagc
     7861 tcataataaa ttattgtgtt accgctattt ttatatttat ggtgaagacg ggcaaaaaat
     7921 aataacgatt tcatctgctt ttgtcttaat ggattttaaa acacggaaga ttcatcctgt
     7981 tcttgatgat attacttcaa tttaccaatc ccaaagaatc aaaaaagtta ttcgagggcc
     8041 gaaatatcat cccattggag atagtaaagt taaacaatac catgttcgtt attttgattt
     8101 agatatgaat ggacatgtta ataatagtaa gtatctagaa tggatgtatg atgtgttgga
     8161 cttagatttt ctaagtagcc acattcctaa aaaaattgat ttaaaatata ttaaggaaat
     8221 tcaatatgga acggatatta agtctcattg gtatcaagat ggtcttgtga cacgtcatga
     8281 tattattggt ggcgatgcta ttcatgctca agctaggatt gaatggcagg aaaaaaagga
     8341 ggattaaatg gcttataaag gatatcttat tgatttagat ggtactattt acaaagggaa
     8401 aagtcgtatc ccagcaggag agcgttttat cgaacgtttg caagaaaaag gcattcctta
     8461 tatgttagta actaataata cgactcgcac accagaaagc gttcaggaaa tgcttcgagg
     8521 cttcaatgtt gaaacaccac ttgaaacaat ctataccgca actatggcta cggttgacta
     8581 tatgaatgat atgaaccgtg gaaaaacagc ttacgttatt ggtgaagaag ggcttaaaaa
     8641 agctattgca gatgcaggtt atgtggagga tacaaaaaat ccagcctatg tggttgttgg
     8701 tttagattgg aatgttactt atgataaatt ggcaactgct acgttagcaa ttcaaaatgg
     8761 tgctttattt attggaacca atccagattt gaatattcct actgaacgtg gtttattacc
     8821 cggtgctggc tccttaaatg ctttattaga agccgcaacg cgtataaaac cagtctttat
     8881 tggaaagcca aatgccatca ttatgaataa ggcacttgaa attttaaata ttcctagaaa
     8941 tcaagcagtc atggtcggtg ataattattt gacagatatc atggctggta ttaataatga
     9001 tattgatact ttgttagtaa caacgggttt tacaacagtt gaagaagtac cagatttacc
     9061 aattcaacca agttatgttt tagcaagttt agatgaatgg acatttaatg aaggataagt
     9121 tattagtagt tttaacttgg atttggatta tatcgttagc aactctagct aca
//
LOCUS       DATRQA010000074         9162 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.074, whole genome shotgun sequence.
ACCESSION   DATRQA010000074 DATRQA010000000
VERSION     DATRQA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.074"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..251
                     /locus_tag="VBD27_001318"
     CDS             <1..251
                     /locus_tag="VBD27_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7025327.1"
                     /translation="VTTQSLSKEITKSGNEKVLTSTNNNSSRVAKIISPKHNGDSVNH
                     TLPSTSDRATNGLFVGTLALLSSLLLYLKPKKTKNNSK"
     gene            461..1369
                     /locus_tag="VBD27_001319"
     CDS             461..1369
                     /locus_tag="VBD27_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7025328.1"
                     /translation="MKTIKDWQINICLATYNGQKYLRQQLDSIIQQGYTDWICLIRDD
                     GSTDDTVAIIKEYVNRDSRFIFINSNDDRKLGSHRSFYELVNYKKADFYVFSDQDDVW
                     KENRLERYLEEAEKFNQELPLLVYSNWTSVDEKLTVLKEHNPATVIQEQIAFNQINGM
                     VIMMNHELAKLWEYRQIGAHDSYVGTLAYAVGNVAYISDSTVLWRRQVGAESLNNYGR
                     QYGVATFWQMINTSFDRASLIFAQVSDKMSLERKLFFSRFIELKNANLIRRIYLLSKL
                     KLRRKSLKETVAMTILLLTGYGKPKA"
     gene            1923..2933
                     /gene="nrdF"
                     /locus_tag="VBD27_001320"
     CDS             1923..2933
                     /gene="nrdF"
                     /locus_tag="VBD27_001320"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO7025329.1"
                     /translation="MQSYYDRSQSPLDYALSEKAFPMRSVNWNKLNDDKDLEVWNRVT
                     QNFWLPEKIPVSNDLNSWRTLDADWQQLITRTFTGLTLLDSVQATVGDIAQIKHSQTD
                     HEQVIYANFAFMVAIHARSYGTIFSTLCTSQQIEEAHEWVVDTESLQARSRILIPFYT
                     GDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGY
                     KYQQKVAKLSVEKQAEMKTFVFDLLYQLIDLEKAYLYELYDGFDLAEDAIRFSIYNAG
                     KFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYIMGITEETLDEDW
                     EF"
     gene            2934..3347
                     /gene="nrdI"
                     /locus_tag="VBD27_001321"
     CDS             2934..3347
                     /gene="nrdI"
                     /locus_tag="VBD27_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO7025330.1"
                     /translation="MDSTVKVVYFSSKSNNTHRFVQKLACSNQRIPSDGSSILVTEDY
                     ILIVPTYAGGGDDTKGAVPKQVVQFLNVRQNREHCQGVISSGNTNFGDTYAIAGPIIA
                     RKLNVPLLHQFELLGTQEDVTRVKELLCQFTRKDK"
     gene            3349..5514
                     /gene="nrdE"
                     /locus_tag="VBD27_001322"
     CDS             3349..5514
                     /gene="nrdE"
                     /locus_tag="VBD27_001322"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO7025331.1"
                     /translation="MTQSDAYLSLNAKTRFRDRTGNYHFTSDKEAVEQYMIEHVEPNT
                     MVFTSLIEKLDYLVSNNYYESDLLKQYNLEFICQIFEHAYAKKFAFLNFMGALKFYNA
                     YALKTEDNRYYLEHYEDRVVMNALFLAAGDEKAAYDLVDDMLANRFQPATPTFLNAGK
                     KRRGEYISCYLLRIEDNMESISRAISTSLQLSKRGGGVALCLTNLREFGAPIKGIKNQ
                     ATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKS
                     LSLGLVIPDITFELAKANKDMALFSPYDIERVYGKPMSDISITEEYETLLANADIRKT
                     FISARKLFQTIAELHFESGYPYILFEDTVNAKNPHKKEGRIVMSNLCSEIAQVNTASQ
                     FSEDLTFTKVGHDVCCNLGSINIARAMDQAADFEKLIANSIRALDRVSRTSDLDSAPS
                     IKKGNAANHAVGLGAMNLHGFLATNHIYYDSQEAIDFTDCFFYAMAYYAFKASNHLAK
                     EKGTFEGFSESSYADGSYFYQYTEQNFEPKTQRVKNLLAEYGLTLPSQEDWRKLVQSI
                     KEIGLANAHLLAVAPTGSISYLSSCTPSLQPVVSPVEVRKEGALGRVYVPAYKIDADN
                     YVYYKKGAYEVGSEAIINIAAAAQKHIDQAISLTLFMTDQATTRDLNKAYIQAFKQKC
                     ASIYYVRVRQDILEGSESYDDMLDDFTSSDLEDCQSCMI"
     gene            5577..8744
                     /locus_tag="VBD27_001323"
     CDS             5577..8744
                     /locus_tag="VBD27_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEO7025332.1"
                     /translation="MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASE
                     DDWFEEDNERKTNVSKENSTVDETVSDLFSDGNSNNSSSKTESVVSDPKQVPKAKPEV
                     TQEASNSSNDASKVEVPKQDTASKKETLETSTWEAKDFVTRGDTLVGFSKSGINKLSQ
                     TSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDE
                     GEIFNAYQLTKLTIPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVK
                     LPDNLKVIGELAFFDNQIGGKLYLPRHLIKLAERAFKSNRIQTVEFLGSKLKVIGEAS
                     FQDNNLRNVMLPDGLEKIESEAFTGNPGDEHYNNQVVLRTRTGQNPHQLATENTYVNP
                     DKSLWRATPDMDYTKWLEEDFTYQKNSVTGFSNKGLQKVRRNKNLEIPKQHNGITITE
                     IGDNAFRNVDFQSKTLRKYDLEEIKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEG
                     AFMNNRIGTLDLKDKLIKIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGALHLMFIG
                     NKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPNLQTIREEAFK
                     RNHLKEVKGSSTLSQITFNAFDQNDGDKRFGKKVVVRTHNNSHMLADGERFIIDPGKL
                     SSTMVDLEKVLKIIEGLDYSTLRQTTQTQFREMTTAGKALLSKSNLRQGEKQKFLQEA
                     QFFLGRVDLDKAIAKAEKALVTKKATKNGHLLERSINKAVLAYNNSAIKKANVKRLEK
                     ELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSD
                     TIGEGQKDAYGNPILNVDEDNEGYHTLAVATLADYEGLYIKDILNSSLDKIKAIRQIP
                     LAKYHRLGIFQAIRNAAAEADRLLPKTPKGYLNEVPNYRKKQVEKNLKPVDYKTPIFN
                     KDLPNEKVDGDRAAKGHNINAETNNSVAVTPIRSEQQLHKSQSDVNLPQTSSKNNFIY
                     EILGYVSLCLLFLVTAGKKGKRARK"
     gene            8757..9146
                     /locus_tag="VBD27_001324"
     CDS             8757..9146
                     /locus_tag="VBD27_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO7025333.1"
                     /translation="MITQEMKEIINSQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGTYYEAAKKWAEGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEIT"
BASE COUNT         3118 a         1435 c         1775 g         2834 t
ORIGIN      
        1 cagttactac tcaatcattg tctaaagaaa taactaaatc aggaaatgag aaagtcctca
       61 cttctacaaa caataatagt agcagagtag ctaagatcat atcacctaaa cataacgggg
      121 attctgttaa ccatacctta cctagtacat cagatagagc aacgaatggt ctatttgttg
      181 gcactttggc attgttatct agtttacttc tttacttgaa acccaaaaag actaaaaata
      241 atagtaaata aaactaagta aactactcgg taatatgatg aaaagttcag tttaacatta
      301 tgttaagctg gacttttagg gtattgactc ttatttttga ttaattttat attgattaac
      361 ggcatataat attataatat tataattacg attagtatgt gatactgttc gctttgtaag
      421 ctttcataag atgaactagc cttaaggagt tttgatagca atgaaaacga taaaagattg
      481 gcaaataaat atttgcttag cgacctataa tggtcaaaaa taccttcgcc agcaattaga
      541 tagcataata caacaaggtt atactgactg gatttgttta ataagggacg atggatctac
      601 tgatgataca gtagctatta ttaaagaata tgttaataga gattcacgtt ttatatttat
      661 taattctaac gatgatagaa agttaggaag tcaccgcagt ttttacgagt tggttaacta
      721 taaaaaagca gatttttatg ttttctcaga ccaggatgat gtttggaaag aaaatcgttt
      781 agaacgttac cttgaagaag cagagaaatt taaccaagaa ttaccattgc ttgtttattc
      841 gaactggact tctgtagatg aaaaattaac agtattgaaa gaacataatc cagcaacagt
      901 tattcaagaa caaattgcct ttaaccaaat aaatggtatg gttatcatga tgaatcatga
      961 gttagcaaag ctttgggaat accgtcaaat tggcgcccat gattcttatg ttggaactct
     1021 ggcttacgcc gttggaaatg tggcttatat ttctgacagt acagtccttt ggcgtagaca
     1081 agtaggggca gagtcgctaa ataattacgg gagacaatat ggggtggcta cgttttggca
     1141 gatgataaat acttcttttg atcgtgcttc gcttattttt gctcaagtat ccgataaaat
     1201 gtccctggag cgtaaactct tctttagtcg ttttatagaa ttaaaaaatg ccaatctaat
     1261 aagaaggatt tatcttttat caaaattaaa acttcgtcgg aaatcattga aagaaacagt
     1321 tgctatgact atcctacttt taacaggtta cggtaaacca aaagcataat ttttttggtt
     1381 gaaggaaatc aagttgagtt gtagttaaaa atatagaaaa tatttatttt tcaaaaagtt
     1441 gttgacactt aacgaaatta gtgtataata atattaaaga acaaggtaga accttggctc
     1501 gcattaattt cgggttttgg aaaaggagta gcaatcgcta ctcctttttt gttttacaca
     1561 ggaaaagtat gaaggaaaag ccttttttct tataaaaatt aagcttgctt aattttttaa
     1621 tttttatatt ctttacatta tgatgaaaac acaagatata gtggtgatat atgttgaaag
     1681 acacaaaact ttgtgttagt atactgttta agcagatcct taataaaggt aaccgacaaa
     1741 aaagtttaac gtgacttaac aaaacagtgg ttttgaagca agagttagct tcttaccaga
     1801 ataaaaaaac atcaagaaga acattccaat agttgtgtga ttttaacaaa aatccagtcc
     1861 tcttgggagg cttttttggt gcttaaaact cgcttaattt taatatttag gagactagtt
     1921 taatgcaatc atactatgat cgttctcaat caccacttga ctatgccttg tcagaaaaag
     1981 cttttcctat gcgttctgtt aattggaata agctaaacga tgacaaagac ttagaggtat
     2041 ggaacagagt aactcaaaat ttctggttac ctgaaaagat cccagtttct aatgacctta
     2101 attcttggag aacattggat gctgattggc agcaattaat tactcggact tttactggtc
     2161 taaccttatt agattctgtg caggctactg ttggtgatat tgctcagatt aagcattctc
     2221 agacagatca tgaacaagtt atttatgcta actttgcctt tatggtagct attcatgcgc
     2281 gatcttatgg aacgattttt tcaaccttat gcactagcca acaaattgag gaagcacatg
     2341 aatgggtagt ggatactgaa agtctacaag ctcgatcacg tatacttatt cctttttata
     2401 ctggtgacga tcctttgaaa tctaaggttg ctgcagccat gatgccagga tttttgttat
     2461 atggtggctt ttaccttcct ttttaccttt cagccagagg aaaattacca aatacgtctg
     2521 atattattcg tttaatttta cgagataagg taattcataa ctattacagt ggctataagt
     2581 atcaacagaa agttgctaaa ttatctgtag aaaagcaagc cgaaatgaag acatttgtct
     2641 ttgacctcct ctatcagttg attgatttag aaaaagccta tctttacgaa ctatatgatg
     2701 gttttgattt ggctgaagat gcaatcagat ttagcattta caatgctggt aaatttttac
     2761 aaaatttagg atatgattct ccatttacag aagaagagac tcgtatatct cctgaggttt
     2821 ttgcacaact ttcggcgcgt gcagatgaaa atcatgattt cttctcagga aacggctctt
     2881 catatattat ggggattaca gaagaaacat tggatgaaga ttgggagttt taaatggata
     2941 gtacagtgaa ggtggtctat ttttcatcga aatctaacaa tacccatcgt tttgttcaaa
     3001 agttggcttg ttctaaccag cgtattccat cagatggtag tagtatattg gttactgagg
     3061 attatatttt gattgtgcct acttacgctg gaggaggaga tgataccaaa ggcgcagttc
     3121 caaaacaagt tgtacagttt ttaaatgttc gacaaaatcg ggaacattgc caaggggtta
     3181 tttcttctgg aaataccaat tttggtgaca cttatgccat tgctggacct attattgctc
     3241 gaaaattaaa tgttcctttg cttcatcaat ttgaattact gggaacacaa gaagatgtta
     3301 caagggtaaa ggagttactt tgccaattca ctagaaagga taaataagat gacacaatca
     3361 gatgcatatc tctcgttgaa cgcgaagaca cgctttagag atcgcacagg taattatcat
     3421 tttacttcgg ataaagaggc tgttgaacaa tatatgatag aacatgttga acctaatacg
     3481 atggtgttca catcactaat tgaaaagcta gattatttgg tttctaataa ctactatgaa
     3541 tcggaccttc taaaacaata taaccttgag tttatttgcc aaatttttga gcatgcatac
     3601 gctaagaaat ttgcttttct aaattttatg ggggctttaa aattttataa tgcttatgct
     3661 cttaaaacag aagacaatcg ttattatttg gaacactatg aagatcgtgt tgtaatgaat
     3721 gctctgtttt tagctgctgg tgatgaaaaa gctgcctatg acctagtaga tgacatgcta
     3781 gcaaatcgct ttcaaccagc aacacctacc tttttaaatg cagggaaaaa acgtcgtggt
     3841 gaatatatct cttgctattt gttgcgcatt gaagacaata tggaatctat ttctcgtgct
     3901 attagtacaa gccttcagtt atcaaaacga ggtggtgggg tagcactttg cttaaccaac
     3961 ttacgggaat ttggtgctcc aataaaaggt attaaaaatc aagctactgg tattgtacct
     4021 gtcatgaaat tactagaaga ttcgttttct tacgccaatc aattaggaca aagacaagga
     4081 gctggtgcag tttacttaca cgctcaccat cctgaagtat taacgttttt agatactaaa
     4141 cgtgagaatg ccgatgagaa aattcgaatt aaatctcttt ccctaggact tgttattcct
     4201 gatattactt ttgaattggc taaggcaaat aaagatatgg ccttgttttc accgtatgac
     4261 attgagcgag tatatggtaa accaatgtct gatatctcta tcacagaaga atatgaaact
     4321 ttattagcca atgcggatat tcgtaaaacc tttattagtg cacgaaaact atttcaaacg
     4381 atagcagagc ttcattttga atctggttat ccttatatct tatttgaaga tactgtaaat
     4441 gctaaaaacc ctcacaaaaa agagggacga attgttatgt ctaatttatg ttctgagatt
     4501 gcacaagtta atacagctag tcaattttca gaagacctga ctttcactaa ggttggacat
     4561 gatgtttgtt gtaatttagg ttctattaat attgcgcgtg ctatggatca agcggcggat
     4621 tttgaaaaat taattgctaa tagcattaga gcccttgatc gtgtatctcg aacttctgat
     4681 ttagatagtg ctccatctat taaaaaaggt aatgcagcta atcatgctgt tggtttaggt
     4741 gctatgaatt tgcatggatt tttggcgact aaccatattt attacgattc tcaagaggct
     4801 attgatttta cagattgctt cttttacgct atggcttatt atgcctttaa agcttctaac
     4861 catttagcaa aagaaaaagg aacctttgag ggcttctcgg aatcaagtta tgctgatggc
     4921 agttatttct atcaatatac cgaacaaaac tttgagccaa aaacacagcg tgttaaaaat
     4981 ctactagctg agtatggtct gactctacca agccaagagg attggagaaa gcttgtgcaa
     5041 agtattaaag agataggatt agctaatgcg catttattag ctgttgctcc gacagggtca
     5101 atcagttatc tttcttcttg tactccgagc cttcaaccgg ttgtatcacc tgtcgaagta
     5161 cgcaaggaag gagcactggg gagggtttat gtacctgctt ataagattga tgcagataat
     5221 tatgtctact acaaaaaagg agcttatgaa gtgggatctg aggcgattat caatattgca
     5281 gctgccgctc aaaaacacat tgatcaagct atttcgttaa cgcttttcat gacagatcaa
     5341 gcaactacgc gagatttaaa taaagcctat attcaagcat ttaaacaaaa atgtgcctct
     5401 atttattatg tacgagtgag acaggacatc ctagaaggta gcgagagtta tgatgatatg
     5461 ctggatgatt tcacttcatc ggacttagaa gactgtcaat cctgcatgat ttaactaaat
     5521 atactcaatc agaagattaa gagaaatata ttggctttta gaaaggcttt attgtaatga
     5581 caaaaaaaca tcttaaaacg cttgccttgg cacttactac agtatcagta gtgacataca
     5641 gccaggaggt atatggatta gaaagagagg aatcggtcaa acaagaacaa acccagtcag
     5701 cttcagaaga tgattggttc gaagaagata atgagaggaa aacaaatgtt tctaaagaga
     5761 attctactgt tgatgaaaca gttagtgatt tattttctga tggaaatagt aataactcta
     5821 gttctaaaac cgagtcagtg gtaagtgacc ctaaacaagt ccccaaagca aaaccagagg
     5881 ttacacaaga agcaagcaat tctagtaatg atgctagcaa agtagaagta ccaaaacagg
     5941 atacagcttc aaaaaaggaa actctagaaa catcaacttg ggaggcaaaa gatttcgtaa
     6001 ctagagggga tactttagta ggtttttcaa aatctggaat taataagtta tctcaaacat
     6061 cacacttggt tttaccaagt catgcagcag atggaactca attgacacaa gtagctagct
     6121 ttgcttttac tccagataaa aagacggcca ttgcagaata tacaagtagg ctaggagaaa
     6181 atgggaaacc gagtcgttta gatattgatc agaaggaaat tattgatgag ggagaaatat
     6241 ttaatgctta ccagttgact aagcttacta ttccaaatgg ttataagtct attggtcaag
     6301 atgcttttgt ggacaataag aatattgctg aggttaacct tcctgagagt ctcgagacta
     6361 tttcagacta tgcttttgct cacatgtctt taaaacaagt aaagttacca gataacctaa
     6421 aggtcattgg agaattagct ttttttgata atcagattgg tggtaagctt tacttgccac
     6481 gtcacttgat aaaattagca gaacgcgctt tcaaatctaa tcgtattcaa acagttgaat
     6541 ttttgggaag taagcttaag gttataggag aagcaagttt tcaagataat aatctgagga
     6601 atgttatgct tccggatgga cttgaaaaaa tagaatcaga agcttttaca ggaaatccag
     6661 gagatgaaca ttacaacaat caggttgtat tgcgcacaag gacaggccaa aatccacatc
     6721 aacttgcgac tgagaatact tacgtcaatc cggacaaatc attgtggcgt gcaacacctg
     6781 atatggatta taccaaatgg ttagaggaag attttaccta tcaaaaaaat agtgttacag
     6841 gtttttcaaa taaaggctta caaaaggtaa gacgtaataa aaacttagaa attccaaaac
     6901 aacacaatgg tattactatt actgaaattg gtgataacgc ttttcgcaat gttgattttc
     6961 aaagtaaaac tttacgtaaa tatgatttgg aagaaataaa gctcccctca actattcgga
     7021 aaataggtgc ttttgctttt caatctaata acttgaaatc ctttgaagca agtgaagatt
     7081 tagaagagat taaagaggga gcctttatga ataatcgtat tggaactcta gacttgaaag
     7141 acaaacttat caaaataggt gatgctgctt tccatattaa tcatatttat gccattgttc
     7201 ttccagaatc tgtacaagaa ataggacgtt cagcttttcg acaaaatggt gcgcttcacc
     7261 ttatgtttat cggaaataag gttaaaacaa ttggtgaaat ggctttttta tccaataaac
     7321 tggaaagtgt aaatctctct gagcaaaaac aattaaagac aattgaggtc caagcttttt
     7381 cggataatgc ccttagtgaa gtagtcttac cgccaaattt acagactatt cgtgaagagg
     7441 ctttcaaaag gaatcatttg aaagaagtga agggttcatc tacattatct cagattactt
     7501 ttaatgcttt tgatcaaaat gatggggaca aacgctttgg taagaaagtg gttgttagga
     7561 cacataataa ttctcatatg ttagcagatg gtgagcgttt tatcattgat ccaggtaagc
     7621 tatcttctac aatggtagac cttgaaaagg ttttaaaaat aatcgaaggt ttagattact
     7681 ctacattacg tcagactact caaactcagt ttagagaaat gactactgca ggtaaagcgt
     7741 tgttatcaaa atctaacctc cgacaaggag aaaaacaaaa attccttcaa gaagcacaat
     7801 ttttccttgg tcgcgttgat ttggataaag ccatagctaa agctgagaag gctttagtga
     7861 ccaagaaggc aacaaagaat ggtcatttgc ttgagaggag tattaacaaa gcggtattag
     7921 cttataataa tagtgctatt aaaaaagcta atgttaagcg cttggaaaaa gagttagact
     7981 tgctgacaga tttagtcgag ggaaaaggac cattagcgca agctacaatg gtacaaggag
     8041 tttatttatt aaagacgcct ttaccattgc cagaatatta tatcggattg aacgtttatt
     8101 ttgacaagtc tggaaaattg atttatgcac ttgatatgag tgatactatt ggcgagggac
     8161 aaaaagatgc atatggtaat cctatattaa atgttgacga ggataatgaa ggttatcata
     8221 ccttggcagt tgccacttta gctgattatg aaggtcttta tattaaagat attttaaata
     8281 gttcccttga taagattaaa gcaatacgcc agattccttt ggcaaaatat catagattag
     8341 gaattttcca agctatccga aatgcagcgg cagaagcaga ccgattgctt cctaagacac
     8401 ctaaggggta cctaaatgaa gtcccaaatt atcgtaaaaa acaagtggag aaaaatttaa
     8461 aaccagttga ttataaaacg ccgattttta ataaggattt acctaatgaa aaggtagacg
     8521 gtgatagagc ggctaaaggt cataatataa atgcggagac taataattct gtagctgtaa
     8581 caccaataag gtccgagcag caattacata agtcacagtc tgatgtaaat ttacctcaaa
     8641 caagttctaa aaataatttt atatacgaga ttctaggata cgttagttta tgtttgcttt
     8701 tcctagtaac tgctgggaaa aaaggaaaac gagcaagaaa ataaggagaa attaatatga
     8761 ttacacaaga aatgaaagag attattaata gtcagttagc aatggtcgcg acggttgatg
     8821 ctaaaggtca acccaatatt ggcccaaaac gttctatgcg tctctgggat gataaaacgt
     8881 ttatttataa tgaaaataca gatggtcaaa ctcgtattaa cattgaagat aatgggaaaa
     8941 ttgaaattgc ctttgttgat cgtgaacgtc ttttaggtta caggtttgtg ggaactgctg
     9001 aaattcaaac agaaggtact tattacgaag cagcgaaaaa atgggcagaa ggacgcatgg
     9061 gggttccgaa agcagttggt attattcatg tcgagcgtat ttttaattta caatctggtg
     9121 ctaatgcagg taaagaaatc acttaatata aaaaagcctc aa
//
LOCUS       DATRQA010000075         9030 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.075, whole genome shotgun sequence.
ACCESSION   DATRQA010000075 DATRQA010000000
VERSION     DATRQA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.075"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(86..1354)
                     /locus_tag="VBD27_001325"
     CDS             complement(86..1354)
                     /locus_tag="VBD27_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO7025334.1"
                     /translation="MKIDKRHLLNYSILIPYLILSILGLIVIYSTTSATLIQLGANPF
                     RSVINQGVFWAVSLVAIIFIYKLKLNFLKNSKVLTMAVLVEVFLLLIARFFTQEVNGA
                     HGWIVIGPISFQPAEYLKVIIVWYLAFTFARRQKKIEIYDYQALTKGRWLPRSLSDLK
                     DWRFYSLFMIGLVIAQPDLGNGSIIVLTVIIMYCISGIGYRWFSALLGLIVVGSTLFI
                     GTIAVVGVETMAKVPVFGYVAKRFNAFFDPFKDLTDSGHQLANSYYAMSNGGWFGRGL
                     GNSIEKLGYLPEATTDFVFSIVIEELGVIGAGFILALVFFLILRIMHVGIKAKDPFNS
                     MIALGIGAMLLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAIGFVLNIDAN
                     EKKELIMKEAEEQYKPQEKNEKIINLDAFK"
     gene            complement(1460..4255)
                     /gene="ppc"
                     /locus_tag="VBD27_001326"
     CDS             complement(1460..4255)
                     /gene="ppc"
                     /locus_tag="VBD27_001326"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEO7025335.1"
                     /translation="MSHPKLESSSNKEIITEEVGLLKQLLDEATQKLIGSESFDKIEK
                     IVSLSSTDDYTGLKETISALSNEEMVIVSRYFSILPLLINISEDVDLAYEINYKNNLN
                     QDYLGKLSTTIDVVAGHENAKDILEHVNVVPVLTAHPTQVQRKTVLELTSKIHDLLRK
                     YRDVKAGIVNQEKWYADLRRYIGIIMQTDTIREKKLKVKNEITNVMEYYNRSLIKAVT
                     KLTAEYKALAAKKGIHLENPKPLTMGMWIGGDRDGNPFVTAETLRLSAMVQSEVIINH
                     YIEQLNELYRNMSLSISLTEVSPELVTLANQSQDNSVYRENEPYRKAFNFIQDKLVQT
                     LLNLKVGSYPKEKFVSRQESSGIVGRYIKSHIAQVASDIQTEELPAYATAEEFKQDLL
                     LVKQSLVQHGQDSLVDGELACLIQAVDIFGFYLATIDMRQDSSINEACVAELLKSANI
                     VDDYSSLSEEEKCQLLLKELTEDPRTLSSTHTPKSELLQKELAIFQTARELKDQLGED
                     IIKQHIISHTESVSDMFELAIMLKEVGLIDANQARIQIVPLFETIEDLDNSRDIMTQY
                     LHYGLVKKWITTNNNYQEIMLGYSDSNKDGGYLSSGWTLYKAQNELTKIGEENGIKIT
                     FFHGRGGTVGRGGGPSYEAITSQPFGSIKDRIRLTEQGEIIENKYGNQDAAYYNLEML
                     ISASIDRMVTRMITNLNEIDNFRETMDGIVAESNAVYRNLVFDNPYFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEISGLRAIPWVFSWSQNRIMFPGWYGVGSAFKQFIEQDEA
                     NLAKLQTMYQKWPFFNSLLSNVDMVLSKSNMNIALQYAQLAGSKEVRDVFNIILNEWQ
                     LTKDMILAIEQHDNLLEENPMLHASLDYRLPYFNVLNYVQIELIKRLRSNQLDEDYEK
                     LIHITINGIATGLRNSG"
     gene            4464..6263
                     /gene="pepF"
                     /locus_tag="VBD27_001327"
     CDS             4464..6263
                     /gene="pepF"
                     /locus_tag="VBD27_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO7025336.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALEDIKVFKKN
                     YEGKLNCVEDFTSALMEIEHIYIQMSHIDTYAFMPQTTDFSNEEFAQISQAGSDFATK
                     ANVLLSFFNTALANADIKILDSLENNPHFKATIRQAKIQKQHLLSPEVEKALTNLNEV
                     LNTPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVTYENYFQNHENAEIREKSFRSFSKG
                     LRKHQNAAAAAYLAKVKSEKLIADMRGYDSVFDYLLSEQEVDRSMFDRQIDLIMDEFG
                     PVAQRFLKHIADVNGIEKMTFADWKLDIDNELNPEVSINDAYDLVMKSVAPLGKEYSQ
                     EVERYQKERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSFFNTHMSTYYVEAPSTFNELLLSDYLENQFDTARQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGAEQLNAIMKEVLTQFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPNGAKEWLAFLKSGGSCTPLETALLIG
                     ADISTDKPLRDTINFLSNTVDQIINYSNLIKQG"
     gene            complement(6322..6711)
                     /locus_tag="VBD27_001328"
     CDS             complement(6322..6711)
                     /locus_tag="VBD27_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEO7025337.1"
                     /translation="MKKYIKLFLLTVFATTLVACGQPSTSNKTTTSSTLEVGKVELVV
                     KEDTNVLSEKVVYHKGDTVLDVLKANYKVKEKDGFITSIDGISQDETKGLYWMFKVNN
                     KLAPKAANQIKVKKNDKIEFYQEVYKK"
     gene            complement(6695..7219)
                     /locus_tag="VBD27_001329"
     CDS             complement(6695..7219)
                     /locus_tag="VBD27_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7025338.1"
                     /translation="MIKNSTLTKLTRVAILSALCVVLRYAFAPLPNIQPITAIFLIPV
                     VLFDLKEGVATVTVTMLVSSFLMGFGPWVFLQIISFTLILCLWKFLIYPLTKAVCFGK
                     ITEVVLQTFFAGGLGVVYGVIIDTCFAWLYHMPWWTYVLAGLSFNMAHALSTCLFYPL
                     LLPILRRFRNEKIH"
     gene            7376..8224
                     /locus_tag="VBD27_001330"
     CDS             7376..8224
                     /locus_tag="VBD27_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEO7025339.1"
                     /translation="MRRRIKPIVVAVFFSLFGLLLIIGHLHSTNTLKKELVEAKKTIP
                     SVKASKVPQKSTSSKDKEFVLKPIIDVSGWQLPKEIDYDTLSKNISGVVIRIFGGSKI
                     SKTNNAAYTTGIDKSFKTHIKEFQKRNIPVAVYSYALGSSVKEMKEEAQIFYKNAAPY
                     KPTFYWIDVEEETMSNMNKGVQAFRKELKRLGAKNVGIYIGTYFMTEQGISVKGFDAV
                     WIPTYGSDSGYYEAAPQTELKYDLHQYTSQGYLPGFNQPLDLNQIAVNKDKKKTYEKL
                     FGKVKD"
     gene            complement(8257..8874)
                     /locus_tag="VBD27_001331"
     CDS             complement(8257..8874)
                     /locus_tag="VBD27_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEO7025340.1"
                     /translation="MEILIAGGSGFLGKQIIKAALTKGHKVAYLSRHEGKGDIFKDPR
                     LTYIKGDITEADKIHLEHRNFDILIDCIGAIKPNQLDELNVKATQKAVALCHKNQIPK
                     LVYISANSGYSAYIKSKRKAEQIIKASGLDYLFVRPGLMYGEERPLSIFQAKCIKLFS
                     HLPFLGIVVQKVFPTKVVIVAEAIVTSLRKKPTQKILSIEELNNK"
BASE COUNT         3003 a         1663 c         1411 g         2953 t
ORIGIN      
        1 tcaaatatct tctctctatt aattagagtt taaatttcaa aaaatacaaa aatcgagata
       61 aaaatctcgt tttttgtttg gatgattatt taaaggcatc aaggttgatt attttttcat
      121 ttttttcttg tggcttatat tgctcttctg cttctttcat tatgagttcc tttttctcgt
      181 tagcatcaat attaagcacg aatccgatag ctacagaaag taccaagagg ctgttccctc
      241 cttgggataa gaaaggaaat gtcactccag ttgaggggat taacccagaa ataccaccaa
      301 tgttaacaaa aacttgcatc aatagcattg ctccaatacc aagagcgatc attgagttga
      361 aaggatcttt agcctttata cctacgtgca taatacgtaa aattaagaag aaaaccaaag
      421 ctaaaataaa ccctgcacca ataactccta attcctcaat aacaattgaa aagacaaagt
      481 ctgttgttgc ttctggtaag taccctaatt tttcaataga attcccaagt cctcgaccaa
      541 accagccacc gttgctcatt gcataatatg aattagccaa ctggtgtcct gaatctgtta
      601 aatctttaaa tgggtcaaaa aaagcattaa atcgcttagc tacataacca aagacaggga
      661 ctttagccat tgtctcaact ccaacaactg caatagttcc gataaataaa gttgacccca
      721 cgacgattag accaagtaaa gctgaaaacc atcgataacc aataccacta atacagtaca
      781 taataatgac cgttagaaca attatcgaac catttcctaa atcaggttgt gcaattacca
      841 aaccaatcat aaataaagaa taaaatcgcc aatcttttag gtcgcttagt gatctcggaa
      901 gccatctccc ttttgtcaaa gcttgataat catatatttc tatctttttt tgccttcgag
      961 caaaagtaaa cgctaaatac catacaatga taacttttaa atattcagca ggttgaaaac
     1021 taataggacc aataacaatc caaccatgtg ctccattaac ttcttgtgtg aaaaagcgcg
     1081 ctattagtag caaaaatact tctaccaata ctgccatagt caacacttta gaatttttca
     1141 aaaaatttag tttcagttta tagataaaga taattgcaac caaactgact gcccaaaaga
     1201 caccctggtt gattactgaa cgaaaagggt tagctccaag ctgaattaga gttgcactag
     1261 ttgtcgaata aattacaatt aagcctagaa tagacaatat caaataggga atgaggatgg
     1321 aataattcag aagatgcctt ttatcaattt tcatattgta ttatttcctt taatatatca
     1381 aaaaattacc attatgcatt atatcatctt tcgaagtaaa gtaaaaggga gaatagcaat
     1441 gatcctccct ctatcaatat taacctgaat ttcgtaaacc tgttgcaata ccattaatag
     1501 taatatgaat caacttctca tagtcttcat ctagttggtt actacgaaga cgtttaatca
     1561 attcaatttg aacataattc aaaacattga aatatggcaa acgataatct aaacttgcat
     1621 gtaacattgg gttttcttct aataaattat catgctgttc aatagctaaa atcatatctt
     1681 tagttaactg ccattcatta aggatgatat taaaaacatc tcgaacttct ttacttccag
     1741 ctaactgagc atactgaagc gcaatattca tatttgattt tgataaaacc atatctacat
     1801 tagataaaag agaattaaag aatggccatt tttgatacat ggtttgtaat tttgctaagt
     1861 ttgcctcatc ttgttcaata aattgcttga aggctgaccc aacaccatac caacccggaa
     1921 acataatacg attttgtgac catgaaaata cccatggaat tgcacgtaaa cctgaaattt
     1981 ctgtaattgt tttacgtgct gctggccgag aaccaatatt taaacttgat acctctttaa
     2041 ttggacttgc ttcaaagaaa tagtcataga aataaggatt atcaaaaact aaatttctat
     2101 aaacggcatt actttcagcc acaataccat ccattgtctc acggaaatta tcaatttcat
     2161 taaggttagt aatcatacgt gttaccatgc ggtctatgga agctgaaata agcatttcaa
     2221 ggttataata ggctgcatct tgattaccat atttattctc aatgatttca ccttgctctg
     2281 ttaggcgaat tcgatctttg attgaaccaa aaggttgtga tgtgatagct tcataagatg
     2341 gaccaccacc acgaccaaca gttccaccac gaccgtggaa aaatgtgatt ttaataccgt
     2401 tttcttcacc tatttttgtt agctcatttt gagctttata tagagtccaa ccagatgata
     2461 aatagccacc atctttatta ctatctgaat aacctagcat tatttcttga taattgttgt
     2521 tagttgtaat ccattttttt actaatccat aatgtagata ctgggtcata atatcacgtg
     2581 aattatccaa atcttcgatg gtttcaaata acggtacaat ctgaatgcga gcttgattgg
     2641 catcaattag tccaacttct tttaacatga tagctaactc aaacatatcc gaaacgctct
     2701 ctgtgtgaga aatgatatgc tgttttataa tgtcttcacc caattgatct tttaactcac
     2761 gtgctgtttg gaaaatagcc aattcttttt gaagtaactc ggatttagga gtatgcgtag
     2821 aagataatgt tctaggatct tctgttaatt cttttaagag aagttgacat ttttcttctt
     2881 ctgaaagaga actgtaatca tctacaatat tggcagactt caataattct gctacacaag
     2941 cttcattaat actagaatct tgacgcatat caatagtagc taaataaaat ccaaagatat
     3001 ctactgcttg aataagacaa gctaattcgc catcgacaag agaatcttgt ccatgttgaa
     3061 ctaaagattg cttcacaagt aacaaatctt gtttaaactc ctcagcagta gcgtatgctg
     3121 gtagttcttc tgtctgtatg tcagaagcta cttgagcgat atgtgatttt atataccttc
     3181 ctacaatacc agaagattct tggcgactaa caaatttctc ttttgggtaa gaaccaactt
     3241 ttaaatttag tagggtttgt actagtttat cttggataaa attgaaagct ttacgataag
     3301 gttcattttc cctatatact gaattatctt gtgactggtt tgccaacgta acaagttcgg
     3361 gagagacttc agttagactt attgaaagtg acatattacg ataaagttca tttagctgct
     3421 caatataatg gttaataatc acttcacttt gtaccatagc tgataatctt agtgtctcag
     3481 cagttacaaa gggattgcca tctctatcac caccaatcca catccccata gtcagaggct
     3541 taggattctc taagtgaata ccttttttag ctgccagtgc cttatattct gctgttaatt
     3601 tggtaactgc tttaataaga gaacggttgt aatattccat tacattggtt atctcatttt
     3661 tgactttcaa ttttttctcg cgaatagtgt ctgtttgcat gataatgccg atgtaacgac
     3721 gcaagtcagc ataccatttt tcttgattca cgattcctgc tttgacatct ctgtatttac
     3781 gtaataaatc atgaatctta gacgttaatt cgagcaccgt tttacgctgc acctgagtag
     3841 gatgagctgt tagcacaggc acaacattga cgtgttcaag aatatcttta gcattttcat
     3901 ggccagctac aacatcaata gtcgttgata actttcctag gtaatcttgg ttaagattat
     3961 ttttataatt aatttcatat gctaaatcaa cgtcttctga aatattaatt aagagcggca
     4021 aaatagagaa gtaacgagaa acaataacca tctcctcgtt gcttaaagcc gaaatagttt
     4081 ccttcaaacc agtataatcg tcagttgatg ataatgaaac tattttttca atcttgtcaa
     4141 atgattcact accaatcaat ttttgcgttg cttcatccaa aagctgcttc agtaaaccga
     4201 cctcttcggt aatgatttct ttattgctac tactttctaa tttcgggtgg gacaaaccat
     4261 cacttcctct cttaaatctc actactatga taccatttta aagtaaaaag taaaccattc
     4321 taggattttt agatagaatt ttcagatttt tacttattta tgacagtaat cagtttttct
     4381 tgttcgactt ttttgatata ctgaggataa ttgagattaa ctgattaatt gttaattaac
     4441 aactattatt aaaggagtat cgcatggaac ttaaaaaacg ctctgaattc cctgaaaatg
     4501 agctttggga tctgactgct ctctacaaag atcgtcaaga ttttttactg gctattgaaa
     4561 aagctcttga ggatatcaag gttttcaaaa aaaattacga gggaaaactt aattgtgttg
     4621 aggattttac tagtgccctt atggaaatcg agcatattta tatccagatg agtcacattg
     4681 atacctatgc ttttatgcct caaactactg actttagcaa cgaagagttt gcacaaattt
     4741 ctcaagcagg atcagacttc gcaactaaag ctaatgtact actttctttc tttaatactg
     4801 ccctagcaaa tgcagatata aagattttag attctttaga aaataaccct catttcaaag
     4861 caactattcg ccaagctaaa attcaaaagc aacacttatt aagtccagaa gtcgaaaaag
     4921 ctttgacaaa cttaaatgaa gtcctaaata caccatatga tatttatact aaaatgcgtg
     4981 ctggcgattt tgatatggaa gattttgaag ttgacggaaa aacctacaaa aatagttttg
     5041 taacttatga aaattacttt caaaatcacg aaaatgcaga gatacgtgaa aaatccttcc
     5101 gttcattctc gaaaggtcta cgtaagcacc aaaatgctgc agctgcggct tatcttgcaa
     5161 aagtcaaatc tgaaaagtta attgctgata tgcgtggtta cgatagtgtt tttgattatc
     5221 ttctttccga acaagaagtt gaccgttcaa tgtttgacag acaaattgat cttatcatgg
     5281 atgaatttgg tccagtagct caacgattcc tcaaacatat cgccgatgtt aatggtattg
     5341 aaaagatgac ttttgcagat tggaaattag acattgataa cgaacttaat cccgaagtaa
     5401 gtatcaatga tgcttatgac ttagtaatga aatcagttgc tcctttagga aaagaatatt
     5461 cccaagaggt tgagcgctat caaaaagagc gttgggttga ttttgcagct aacgctaata
     5521 aagattctgg aggatatgct gcggatcctt acaaagttca tccctatgta ctcatgagct
     5581 ggacggggcg tatgagcgat gtctacacac ttattcatga gattggacat tctggtcaat
     5641 ttatcttctc agataatcac caaagttttt tcaatactca catgtccact tactatgttg
     5701 aagctccatc tacttttaac gaacttttac ttagtgacta cttagaaaac caatttgata
     5761 cagcacgtca aaaacgcttt gctcttgcac atcgcttaac tgatacttac ttccacaatt
     5821 tcattactca cttacttgaa gctgctttcc aacgtaaagt ttacactcta attgaggagg
     5881 gagaaacttt tggagcagaa caattaaatg ctattatgaa ggaagtatta acacaattct
     5941 ggggagatgc tattgaaata gatgacgatg ctgctttgac atggatgaga caagctcatt
     6001 actacatggg gctctatagc tatacttact ctgctggttt agtaatctcg acagccggct
     6061 atctcaatct caaaaataat ccaaacggag caaaagagtg gttagcattc ttgaaatctg
     6121 gtgggagctg taccccttta gaaacagccc tattaatcgg tgctgatatt tctactgata
     6181 agcctctacg cgatactatt aatttcttat ctaacactgt agatcaaatt attaattata
     6241 gtaatcttat taaacaaggc taaactacag tctttcaaaa acataaaaac aagttggaga
     6301 tatctccaac ttgtttttat cttatttttt gtaaacttcc tgataaaatt caattttgtc
     6361 gttcttttta actttaattt ggtttgctgc cttaggagct aatttattat tgaccttgaa
     6421 catccaataa agccctttgg tttcatcttg gctgatgcca tcaattgaag taatgaaacc
     6481 atctttttct ttaactttat agttagcttt aagcacatcc aaaacagtgt cacctttatg
     6541 gtaaacaacc ttttcactta acacgttcgt atcttcttta acaaccaatt caaccttacc
     6601 aacttctaac gttgagctag ttgttgtctt attcgatgta gatggttgcc cgcaagctac
     6661 taaagtcgta gcgaatactg ttagcaagaa taatttaatg tattttttca ttcctaaatc
     6721 tccttaaaat cggtaacaaa agaggataaa ataaacaagt tgataaagcg tgtgccatat
     6781 taaaactaag tcctgctaat acataagtcc accaaggcat atgatataac caagcaaaac
     6841 aggtatcaat aatgacgcca taaacaaccc ctaaccctcc tgcaaagaaa gtttgaagta
     6901 ctacctccgt tattttacca aaacatacag cttttgtcag cggatatatc agaaatttcc
     6961 acaagcataa aattaaagtg aaggaaatga tttgcaaaaa gacccatggc ccaaagccca
     7021 ttaaaaaaga agaaactagc attgttacag ttacagtagc tacaccttct tttaaatcga
     7081 ataagacaac tggaatcaaa aaaatggcag taattggctg aatgttaggc agaggcgcaa
     7141 aagcatatct caacacaaca cacaaagcag ataatatagc tactctagtc aatttagtta
     7201 aagttgaatt ctttatcatg tctttaatta tacctcaaaa ctatgactac cgcttaaaag
     7261 tcttttttat tacaatttca tttctaatgt tacataaacg acctcttttt ttaaaagaac
     7321 gtaaataagc ctgtaaactt tgatttttta aaccttttaa gttacaatat caataatgag
     7381 aagaagaatt aaacctattg ttgttgcggt ttttttcagt ttatttggac ttttattaat
     7441 tattggtcat ttacattcga caaatacttt gaaaaaagaa ttagttgaag ctaaaaagac
     7501 aattccatcc gtaaaagctt caaaagtacc gcaaaaatca acatcatcga aagataaaga
     7561 gtttgttctt aaaccgatta tcgatgtctc tggttggcaa cttcctaagg agattgatta
     7621 cgatacgctt tcaaaaaata tttcaggtgt tgttattcgt atctttggtg gatcaaagat
     7681 atctaagact aataacgctg cttatacaac tggaatcgat aaatcgttta agacccatat
     7741 caaagaattt caaaagcgaa atatcccagt agctgtctac agttatgcac ttggttcaag
     7801 tgttaaagaa atgaaagaag aggctcagat attttataag aatgcagctc cttacaaacc
     7861 aactttttat tggattgacg tagaagagga gacaatgtct aacatgaata aaggtgtcca
     7921 agcattccga aaagagttaa aaagacttgg tgctaaaaat gttggtatct acatcggtac
     7981 ttactttatg actgagcaag gtatctctgt aaaaggattt gacgctgttt ggattccaac
     8041 ttatggtagc gattctggat actatgaagc agctccacaa actgaactta aatacgattt
     8101 acaccaatac acctctcaag gttatctacc aggattcaat caaccgcttg atttaaatca
     8161 aattgcagtt aataaagaca agaagaaaac ttatgagaaa ctttttggaa aagtaaaaga
     8221 ttaaaagcta ctgttaacag tagctttttt gataaactat ttattattta attcttcaat
     8281 agaaaggatt ttttgagttg gttttttcct aagcgaagta acgattgctt ctgccactat
     8341 cacaacctta gttggaaaga ctttttgtac aacaatacct aagaaaggca aatgactaaa
     8401 taattttata cacttggctt ggaaaatcga gagaggtcgc tcttcaccat acatcaaacc
     8461 tggtcttaca aaaagataat ccagaccgct tgctttgatt atctgctctg ccttcctttt
     8521 acttttaatg taagctgaat agccgctatt ggctgaaatg taaactaact ttggtatttg
     8581 atttttgtga cagagtgcta ctgctttttg ggttgcttta acgttaagct catctagttg
     8641 attgggctta atcgctccaa tacagtcaat taatatatca aaatttctat gttctaaatg
     8701 aatcttatca gcttctgtaa tatctccctt aatgtaggtt aatctaggat ccttaaatat
     8761 atcaccttta ccttcatgcc ttgataagta agccacctta tgcccttttg taagcgctgc
     8821 ttttattatc tgcttcccta gaaaaccact accacctgca atcagtattt ccatagttcc
     8881 ctctccttca ttttgtaaat ttagtataga aatttttata tgccttgtca aagtaatcgt
     8941 ttaatacctg accagaatat gatttcaagt cattacatta ggacacttca tatttagcta
     9001 tttataaaac ttctaattta acattccaaa
//
LOCUS       DATRQA010000076         8862 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.076, whole genome shotgun sequence.
ACCESSION   DATRQA010000076 DATRQA010000000
VERSION     DATRQA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8862
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.076"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            121..660
                     /locus_tag="VBD27_001332"
     CDS             121..660
                     /locus_tag="VBD27_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEO7025341.1"
                     /translation="MKTLKKLLSNYKFDIKKFKFGMRTFKTGLSVFLVLLVFHLFGWN
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMINVACNNKSGIIGGTAALLIITLSIPSGETILYVFARIFETFCGVFIA
                     MMVNTDIEILRKKLKNNKL"
     gene            740..1111
                     /locus_tag="VBD27_001333"
     CDS             740..1111
                     /locus_tag="VBD27_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7025342.1"
                     /translation="MKERELRRTMAVFPIGAVMKLTDLTARQIRYYEDQGLITPERTE
                     GNRRMFSLNDMDRLLEIKDFISDGLHISDIKNEYMQRQHKSKEKQKSLSDAEVRRLLQ
                     DELRNQGRFSSPSQHIGNMHL"
     gene            1145..2491
                     /gene="glnA"
                     /locus_tag="VBD27_001334"
     CDS             1145..2491
                     /gene="glnA"
                     /locus_tag="VBD27_001334"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO7025343.1"
                     /translation="MTITAEDIRREVKEKNVTFLRLMFTDILGVMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     EPFAGDPRGNLKRNMKRMQEMGYKSFNLGPEPEFFLFKMDENGNPTLDVNDKGGYFDL
                     APTDLADNTRREIVNVLTQMGFEVEASHHEVAVGQHEIDFKYDDVLKACDNIQLFKLV
                     VKTIARKHGLYATFMAKPKFGINGSGMHCNMSLFDNEGNNAFFDPEDPRGMQLSEDAY
                     YFLGGLMKHAYNYTAIINPTVNSYKRLVPGYEAPVYIAWAGRNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALSVLLGSGLEGIENKIEAPEPIETNIYAMTVEERRQAGIVDL
                     PSTLHNALKALEEDEVVKAALGTHIYTNFLDAKRIEWASYATYVSQWEIDNYLDLY"
     gene            2639..3148
                     /locus_tag="VBD27_001335"
     CDS             2639..3148
                     /locus_tag="VBD27_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7025344.1"
                     /translation="MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVD
                     YLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGNWWGNGGDWAASAASAGYTVGTQ
                     PRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTVTP
                     GVVSYIYPN"
     gene            complement(3306..4985)
                     /locus_tag="VBD27_001336"
     CDS             complement(3306..4985)
                     /locus_tag="VBD27_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO7025345.1"
                     /translation="MSNINLKPEEVGVYAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENIDRIKALVITHGHEDHIGGIPFLLKQANLPIYAGPLA
                     LALIKGKLEEHGLLRDATLYEIHANTELTFKNLSVTFFRTTHSIPEPLGIVIHTPQGK
                     VICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKIVGQSIM
                     KIIEGIEGRIIFASFASNIFRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKVHAGLAVDTGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRHDLSEDGVVLAVATVDFDSKMILAGPDILSRGFIYMRESGDLIRESQH
                     ILFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDK"
     gene            complement(5039..5269)
                     /locus_tag="VBD27_001337"
     CDS             complement(5039..5269)
                     /locus_tag="VBD27_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEO7025346.1"
                     /translation="MIYKVFYQETKERNPRREQTKTLYVTIDAANELEGRIAARKLVE
                     ENTAYNIEFIELLSDKHLEYEKETGVFELTEF"
     gene            5448..6140
                     /gene="tsaB"
                     /locus_tag="VBD27_001338"
     CDS             5448..6140
                     /gene="tsaB"
                     /locus_tag="VBD27_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO7025347.1"
                     /translation="MMKVLAFDTSSKALSVAVLNNMECLATVTINIKKNHSINLMPAI
                     DFLMQSIDLEPQDLDRIVVAEGPGSYTGLRVAVATAKMLAYTLKIDLVGVSSLYALTN
                     GFSENDLLVPLIDARRNNVYVGFYQNGDTVKPDCHTSLEEVLQEVGNKANVHFVGEVA
                     AFFDQIKKALPHAKITETLPCAVAIGRKGQKMKSVNVDAFVPRYLKRVEAEENWLKNH
                     CETNTEEYIKRV"
     gene            6142..6549
                     /gene="rimI"
                     /locus_tag="VBD27_001339"
     CDS             6142..6549
                     /gene="rimI"
                     /locus_tag="VBD27_001339"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO7025348.1"
                     /translation="MESSEQALAIWSVLSDVYDKSPWSLSQISEDLKKDSTDYFFVYN
                     DGEVIGFLALQQLVGEVEITNIAVKKNYQGKGYAYQLMSMIADIEVPVFLEVRYSNIV
                     AQKLYERCGFVVLRKRKNYYHDPIEDAIVMRKG"
     gene            6625..7635
                     /gene="tsaD"
                     /locus_tag="VBD27_001340"
     CDS             6625..7635
                     /gene="tsaD"
                     /locus_tag="VBD27_001340"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO7025349.1"
                     /translation="MKDRYILAVESSCDETSVAILKNDKELLANIIASQVESHKRFGG
                     VVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFA
                     WANKLPLIPINHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSEPGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSA
                     FINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKYPVKTLVVAGGVAANQ
                     GLRERLATDISPDIDVVIPPLRLCGDNAGMIALAAAIEFEKENFASLKLNAKPSLAFE
                     SL"
     gene            7755..8780
                     /locus_tag="VBD27_001341"
     CDS             7755..8780
                     /locus_tag="VBD27_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEO7025350.1"
                     /translation="MLHFENDYNKGAHPELLNALIETNDQGLSGYGTDSYCQQAADKI
                     REVCSCPQAEVEFLVGGTQTNQVVISSMLASYEGVIAAETGHVSSHEAGAIEFSGHKV
                     LTLPSHNGKLLASEVATYIETFYGDGNYQHMVFPGMVYISHPTEYGTLYSKAELEELS
                     KICKHYQIPLFIDGARLGYGLAAKDTDVDFPTIAALSDVFYIGGTKMGALAGEAVVFT
                     KKNRPKQFTTIVKQHGALLAKGRLLGLAFDRFFTDNLYLKIGKHAIDLAEELKIILEE
                     KGYSFYLKSPTNQQFIIVENTKLADLAKNVAYSFWEKYDDHHTVIRLATSWSTSREDV
                     TALRNVL"
BASE COUNT         2844 a         1390 c         1803 g         2825 t
ORIGIN      
        1 caaaagttta tgacgatttg atataaattg gtttataaaa gatatactag taataaataa
       61 aacgtttaca aaaagggttt tatggattga tatgttagaa tatatagtga agggataggt
      121 atgaaaacgt taaaaaaatt attatctaat tacaagtttg atattaaaaa gtttaaattt
      181 ggaatgagaa catttaaaac aggcttatcg gtctttttag ttttattggt ttttcattta
      241 tttggttgga acgggcttca aattggtgct ctaactgctg tatttagttt aagagaagac
      301 tttgataaaa gcgtgcactt tggtttttct agaattattg gtaatagtat aggtggctta
      361 ctctccttag tattttttgc attcaatgag atatttcatc aagctttttg ggtcacttta
      421 ttaattgttc ctatttgtac catgttatgt atcatgatta atgttgcttg taataataaa
      481 tcaggtatta ttgggggaac agccgcatta cttattataa ctttatctat cccaagcgga
      541 gagacaattc tatatgtttt tgcacgtatt tttgaaactt tttgtggtgt ttttattgca
      601 atgatggtta atacagatat tgaaatactt cggaaaaaac taaaaaataa caaattatag
      661 tttgatgtca taaaatctca catgagagtt gacatcaaac tttttaaagt ttataatggt
      721 ttaatgaaag gaggccatca tgaaagaaag agaacttagg cgtacaatgg ctgtattccc
      781 gattggagct gttatgaagt tgacagattt gactgccaga caaattcgct attatgagga
      841 tcaagggtta atcacacctg aacgtactga aggtaaccgc cgcatgtttt cactgaatga
      901 tatggaccgc ttactggaaa taaaagactt cattagcgat ggtttgcata tttctgatat
      961 taaaaatgaa tacatgcaaa gacaacataa gtccaaagag aaacaaaagt cattatcaga
     1021 tgccgaggtt cgtcgacttt tacaagatga gctccgtaat caagggcgtt tttcaagtcc
     1081 ttcacagcac attggaaata tgcatttgta aacatgcagt caagctagat attaaggaga
     1141 ctatatgact atcacagcag aagatattcg tcgcgaagtt aaggaaaaaa atgttacctt
     1201 cctccgtttg atgttcacag atatcttagg agtaatgaaa aatgtcgaaa ttccggctac
     1261 agatgaacaa ttagacaaag ttttatcaaa taaagcaatg tttgatggat cttctattga
     1321 gggctttgtt cgtatcaatg aatctgatat gtatttatat cctgatttag atacttggat
     1381 tgtctttcct tggggagatg aaaatggagc tgttgcaggt ctaatctgtg atatctatac
     1441 agcagaagga gaaccttttg ccggagatcc acgtggaaat cttaagcgta atatgaaacg
     1501 tatgcaagag atgggctata agtcatttaa cttaggacca gaacctgaat ttttcctatt
     1561 taaaatggat gaaaatggta acccaacact tgatgttaat gacaaaggag gctactttga
     1621 cttagctcca acagacttag cagataatac acgtcgtgaa attgttaatg tcttaacaca
     1681 aatgggcttt gaagttgaag ctagtcatca tgaagtagca gttggacagc atgaaattga
     1741 ctttaaatat gatgatgttt taaaagcctg tgacaacatt caattattta aattggttgt
     1801 taaaacaatt gctcgtaagc atggtttata tgctactttt atggcaaaac ctaagtttgg
     1861 tatcaatggt tcaggtatgc attgtaatat gtctcttttt gataatgaag ggaacaatgc
     1921 tttctttgat ccagaagatc cacgtggaat gcaattatca gaggatgcct actacttttt
     1981 aggtgggtta atgaaacacg cttacaatta cacagctatc attaatccaa ctgttaattc
     2041 ttataaacgt ttggtaccag gatacgaagc accagtttac attgcttggg caggtcgcaa
     2101 tcgttcgcca cttattcgtg tacctgcatc tcgaggtatg ggaacacgcc tagaattgcg
     2161 ctcggttgac ccaacagcaa atccttatct tgctttatca gtacttttgg gatcaggact
     2221 tgagggaatc gagaataaaa ttgaagcacc agaaccaatt gagacaaata tctatgctat
     2281 gactgtagaa gaacgtcgtc aagcaggtat tgttgattta ccatctactc ttcataatgc
     2341 tcttaaagca cttgaagaag atgaagtggt aaaagcggcc ctaggaactc atatctatac
     2401 taatttctta gacgctaagc gtattgaatg ggcaagttat gcgacttatg tttcacaatg
     2461 ggaaattgat aattacctag atttatatta atcataagaa tgtttataac tagttttagg
     2521 ctttattttg taaagtctaa aactagtttt tttgtaaatt attgtaaaaa attgcctaaa
     2581 acttgttaca ggttttcatt tgtctgttat atggatttga taaactagga gtcatcagat
     2641 ggaggaaaac atgaacataa aacaattaaa gtctaaaaca atgcttggaa cagtggcttt
     2701 ggtgtccgct ttttcatttg cttcaacaaa tgcagatgcg aatacatata actatgcagt
     2761 agatgtagac tacctagcaa gtgcggagga aattgctcaa gcacatccag ctagtaatac
     2821 gtttccacta ggtcaatgca catggggtgt aaaggagatg gcaacatggg caggtaactg
     2881 gtggggaaac ggtggtgatt gggctgcgag cgccgcatca gctggttata cagtagggac
     2941 acaaccgcgc gtgggttcta tcgtatgttg gactgatggt agttatggac atgtggctta
     3001 tgttacagct gtagatcctg tcactaataa aattcaggtt ttagaatcta attatgcagg
     3061 tcatcaatgg attgataatt atcgtggttg gtttgatcca caaaatacag taacaccagg
     3121 ggtagttagc tacatttatc cgaattaatc tataaagaac tcctcacata gacggtataa
     3181 aagtacgata ctgtttggag ttcttttata ttctatagtt atcccagaag taatttgata
     3241 tattaactta ttttgctaat ataggcaatt aaaaagaacg gagaaagtat tttccgttct
     3301 tttgtttatt tatcaggggt aaggaccata ggaataatga taggctcacg ctcagttttt
     3361 tcatataaga atgggcgtaa ggcattaaca atagctccat ttactgattg gatacttgca
     3421 tctttatttt tcagtgcaat acgaatagca ttgaataaga tatgttggct ttcgcgaata
     3481 aggtccccag attctctcat atagataaag cctcggctaa ggatgtctgg tccagctaaa
     3541 atcatttttg aatcgaaatc aacggtagca acggcaagga caacaccatc ttcagataag
     3601 tcgtggcggt cacgtagaac tgccgcacca atatcgccaa taccattccc atcaacgtag
     3661 atatcttgag cattgaaatg tccagcaata cgagcagagt cgctcgttaa agctaaaaca
     3721 tcaccgtttt ccataatgaa gatattctct ttaggaatac ctgtgtcaac tgctaaacca
     3781 gcatggactt tttgcatgcg atactcaccg tgtacgggca tgaaatactt tggtttaatc
     3841 aagcgtaaca ttaatttttg ttcttgttga ccaccatggc cggaggtatg gatattgtta
     3901 attttaccat gaataacatc gacaccagct tcttgaatgg tattgattaa tttattgaca
     3961 cttgttgtat taccagggat tgggcttgat gagaaaatca cagtatcacc aggctgcaaa
     4021 gtaacttgac gatgagtacc attagcgata cgagcaagtg ctgccataga ctcaccttga
     4081 ctacctgtac acataattaa aacttcacta gcatgaagat ttttaagttc gcttggctca
     4141 atgaaggtac cttttggaac tttaatatag ccaagttcaa taccgttaac aattgccttt
     4201 tccattgacc gtccaaaaac agcaatttta cgaccagttt taacagcagc ttcagcagcc
     4261 tgttggagtc ggaagatgtt tgaagcaaaa gaggcgaaaa taatacgtcc ctcgatacct
     4321 tcgataatct tcataataga ttgcccaaca attttttcgg aattagtgaa ggttggaact
     4381 tcagcatttg tcgagtctga taaaaggcag aggacaccat cttcacctag agcagccata
     4441 cgatgtaagt cagcgggctc cccaacaggt gtaaagtcaa atttgaagtc acctgtacaa
     4501 ataaccttac cttgaggggt atgaataaca atacctaatg gttcaggaat ggagtgagta
     4561 gtgcggaaaa atgtaacgct taagttttta aaagtcagct ctgtattagc gtggatttcg
     4621 tataatgtag cgtcacgaag taaaccatgt tcttctaact tacctttgat tagagcaaga
     4681 gcgagaggac ctgcataaat cggtaaatta gcttgtttta atagaaaagg aataccaccg
     4741 atgtggtcct cgtgcccatg agttataaca agggccttga tacggtcaat gttttcaacg
     4801 atatatgaat agtctgggat aacataatct attcccaaaa gatcatcttc agggaattta
     4861 ataccagcgt ctacaataat aatttcgtct tgatattcga taccgtaagt attttttccg
     4921 atttcaccga gaccaccaat cgcataaacc ccaacttctt cgggttttaa gttgatattt
     4981 gacataataa gtttatcata atgggtagat tactctagct attatgttta actcctttct
     5041 agaactcagt gagctcgaaa acaccagttt ctttttcata ttcaaggtgt ttgtctgaaa
     5101 gtaactcgat gaattcgata ttgtaagctg tattttcttc aactagttta cgtgcagcaa
     5161 tgcgaccttc taattcatta gctgcatcaa tagtaacgta aagtgttttg gtttgctcac
     5221 gacgtgggtt gcgttctttt gtttcttgat agaaaacttt ataaatcata ataatccttt
     5281 cttatctctc ggtttttcta actataaaaa aatgggtcaa tgccgttaag tgagacaatg
     5341 acaaaccatt attaagtttt tatagagtaa tagaattaac cttgattcgt tgcaattatc
     5401 atgattatat cataaaataa gaggtagaat caagtaaggg aagttaaatg atgaaagttt
     5461 tagcctttga tacttcaagc aaagcactat cagtggctgt actaaacaat atggaatgtt
     5521 tagcgactgt cactatcaat atcaaaaaga atcatagcat taatttgatg ccagccattg
     5581 attttttaat gcaatcaatt gatttagaac ctcaagattt ggaccgtatc gtagtagcag
     5641 agggtccagg atcttatacg ggcttacgtg tagctgttgc tacagcaaaa atgctagctt
     5701 atacgcttaa gattgactta gttggagtat ctagcctgta cgctttaaca aatggatttt
     5761 cagaaaatga tttattggta ccacttatag atgcacgacg taacaatgtt tatgttggtt
     5821 tctatcaaaa tggtgatact gttaaaccag actgtcacac ttctcttgaa gaagtcttac
     5881 aagaggtggg gaataaagcc aatgttcatt ttgtcggaga ggttgcagca ttttttgatc
     5941 agattaagaa agccttacca catgctaaaa ttacagaaac tttaccttgt gcagtagcaa
     6001 ttgggcgcaa aggacaaaaa atgaaaagcg ttaatgtaga tgcgtttgtt ccacgatact
     6061 taaaacgtgt tgaagctgag gaaaattggt taaaaaacca ctgtgaaacg aatacagaag
     6121 aatatattaa gagagtttga catggaatca tcagaacaag cactggctat atggagcgta
     6181 ctatctgatg tctatgacaa atctccgtgg agtttatcgc agatttcaga agatttaaaa
     6241 aaagattcaa cagattattt ttttgtttat aatgacggtg aagtcattgg ttttttggct
     6301 cttcaacaat tggtaggtga agtagaaatt actaatattg ctgttaaaaa aaattatcaa
     6361 ggtaaaggtt atgcttacca gttaatgtca atgatcgccg atatagaggt gccagttttt
     6421 cttgaggttc gttattcaaa tatcgtagca caaaaacttt atgagaggtg tggttttgta
     6481 gttttaagaa agcgcaaaaa ttattaccat gatccaattg aagatgctat agtgatgcgc
     6541 aagggataaa tagttgatga tagaaaggat atgaatagtc ctcaatacta atggaaaaga
     6601 cgatattgat ggattaatat cattatgaaa gatagatata ttttagcagt ggaaagttca
     6661 tgtgatgaaa ctagtgttgc tattttaaaa aatgataaag agttactagc taatattatt
     6721 gcaagtcaag ttgaaagtca caaacgtttt ggtggtgttg ttcctgaagt ggcaagccgt
     6781 catcacgttg aagtagtaac aacctgtttt gaggatgctc ttcaagaagc aggtattgtt
     6841 gctagcgatt tggatgctgt tgctgtaaca tatggtccgg gattagtagg agccttattg
     6901 gtaggtatgg ctgcagcaaa agctttcgct tgggcaaata aattacctct aattcctata
     6961 aaccacatgg caggtcattt aatggcagca cgtgacgtta aggaacttca atacccattg
     7021 ttagctttgc ttgtcagtgg gggacataca gaattagtat atgtttctga accgggagat
     7081 tacaaaatag taggagaaac tcgggatgat gctgttggag aagcttatga taaagtaggc
     7141 cgtgttatgg gcttaactta tccagcaggt cgcgagattg atcagttagc tcataagggt
     7201 caagatactt accattttcc tagagcgatg atcaaagaag atcatcttga attttctttt
     7261 tctggattaa aatctgcatt tatcaattta catcataatg cagaacaaaa gggtgaagca
     7321 ttggttcttg aagatttatg tgcttccttt caggcggctg ttttggatat tttattggcc
     7381 aaaactcaaa aagctttgtt aaagtatcca gtgaaaactt tagtcgttgc tggtggagtt
     7441 gcagctaatc aaggacttcg ggaacgcttg gctactgata tttctcctga tattgatgtg
     7501 gttattcctc ctcttagatt atgtggggat aatgcaggaa tgattgcatt agcagcagcg
     7561 atagagtttg aaaaagagaa ttttgcttct ttaaaattga atgccaaacc tagtttagct
     7621 tttgagagtt tataggtgtt cataatatca aaacgcttgt tttgatattt tttatttgtt
     7681 ataatgattg aatattcaaa aatattagca aatgatattg tcgctatttt tttaactaat
     7741 aaaggagtta aggaatgtta cattttgaaa atgattataa taaaggagct catccagagc
     7801 ttttaaatgc ccttattgaa actaatgatc aaggtctatc gggatatggg acagatagtt
     7861 actgtcaaca agcggcagat aaaattcgag aagtctgttc ctgtccacaa gctgaagttg
     7921 agtttttagt gggtggtact caaacaaatc aagtagttat cagtagtatg cttgcttctt
     7981 atgaaggagt tattgctgca gaaacaggtc atgtgagtag tcatgaagca ggagccatag
     8041 aattttcagg gcataaggta ttgactttac cttctcataa tgggaaatta ctcgcaagcg
     8101 aagtcgcaac ttatattgag actttttatg gtgatggtaa ttaccaacac atggtttttc
     8161 caggaatggt ttatatatca catccaacag aatatggaac actttatagt aaagcagaac
     8221 tagaagagtt atcgaagatt tgtaaacatt atcaaatccc cttatttatt gacggtgctc
     8281 gtctggggta tggtttggct gcgaaagata cagatgttga tttcccaacg attgcagcct
     8341 tatcagatgt tttttatatt ggtggaacca aaatgggggc tcttgctggg gaagcagttg
     8401 ttttcacaaa gaaaaatcgt ccaaaacagt ttactacaat tgtaaagcag catggtgcgt
     8461 tattagccaa gggaagattg ctggggcttg cttttgatcg tttctttacg gataaccttt
     8521 acttaaaaat tggaaagcat gcgatcgact tagcagagga attaaaaatc attttagaag
     8581 aaaaaggtta ctcattctat ttaaaatcac caactaatca acaatttatt attgtagaaa
     8641 atacaaaact agcagattta gcaaagaatg tagcttatag tttttgggaa aaatatgatg
     8701 accatcatac tgtaatacgt ctagcaacta gttggtcaac tagtcgcgaa gatgtaactg
     8761 ctttaagaaa tgtcttgtga gtttttaata aaagaaatat tattcagtag tgactattac
     8821 aaaataatag tcttgttttt gggattaact aaaagaattt ta
//
LOCUS       DATRQA010000077         8813 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.077, whole genome shotgun sequence.
ACCESSION   DATRQA010000077 DATRQA010000000
VERSION     DATRQA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8813
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.077"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            124..1209
                     /locus_tag="VBD27_001342"
     CDS             124..1209
                     /locus_tag="VBD27_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HEO7025351.1"
                     /translation="MQRKSYLKSMSVLTLTACLISGYVVKDIAMLHAVSASEKKANNV
                     SPRENLYRAVNDNWLANTKLKQGQTSVNSFSEIEDKLKQLLVSDMAKMASGKIETTND
                     EQKKMVAYYKQGMDFKTRDKNGLKPLKPVLQKLEAVSSMKDFQSLAHDFVMSGFVLPF
                     GLTVETNARDNSQKQLVLRQAPALLESPDQYKKGNKEGEAKLSAYRTSAMALLKQAGK
                     SNIEDRKLVKQAIAFDRLLSEKTQVDQSKITAESETAAGRYNPESMETVHNYAKEFDF
                     KELIEKLVGPTNKAVNVEDKTYFKQVNDVINSKQLANMKAWMMISMLVDQSDFLGEQN
                     RQAASAFKNVASGLTQIESKEKMLTPN"
     gene            1230..2048
                     /locus_tag="VBD27_001343"
     CDS             1230..2048
                     /locus_tag="VBD27_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEO7025352.1"
                     /translation="MGDYYGKKYFGEAAKKDVEHMAKKIINVYKTRLKNNTWLSENTK
                     AMAIKKLDNMRLMIGYPEDYPDLYRQYQFDSKASFFENNDNYRKLSNKKTFEEFNQSN
                     QREHWQMSANAVNAYNDPNTNSIVFPAAIFQSPLYDKTKTVSQNYGAIGAIIGHEISH
                     SFDINGMKYDEKGNLHDWWTKEDLKHYKKKTQAMIDQWDGLKADGGKVDGKLTLAENI
                     ADNGGVMASLEALKTEKIQTIKNFLNHGQVFGVKKQPKNKVSPQFSQMFMHHMN"
     gene            2187..3218
                     /locus_tag="VBD27_001344"
     CDS             2187..3218
                     /locus_tag="VBD27_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7025353.1"
                     /translation="MKNITKLSTVALSLLLCTACAASNTSTSKTQSHHPKQTKLTDKQ
                     KEEPKNKEAADQEMHPQGAVDLTKYKAKPVKDYGKKIDVGDGKKMNIYETGQGKIPIV
                     FIPGQAEISPRYAYKNLIERLSKKYKIYTVEPLGYGLSDIPTKPRTLENITKEIHTGL
                     NKIGVKNFYLAAHSLGGMYSLNYAKNYPEEVRGFIGMDTSTPWMEGEQKTKYDPESAK
                     WAMKLPDVDDKTNEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVKFPKGMPTKYFL
                     ADQSVEGIKLRQADFKGLKNWDQQHIDLSEDPKDASVEILKGSHNIFQTQYEHMAKAI
                     DGFIQSHQK"
     gene            3253..4272
                     /locus_tag="VBD27_001345"
     CDS             3253..4272
                     /locus_tag="VBD27_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO7025354.1"
                     /translation="MNKITKLSTVALALMLCVGCSANKDNQKTKTEQPKQEKSIAKQK
                     QEREAEKAMIAKGAIDLTKYKAKPIEDYGQKIDVGDGKKMNIYTMGQGKIPLVVFPGQ
                     AEISPRYAYKNLLDRLGKKYKIYTVEPLGYGLSDMTDKPRTIENITKEIHTALEKVGV
                     KNFYLMAHSLGGIYGLNYAKTYSNDVKGFIGMDTSTPNMEGDGTTGESSASTLKWAES
                     IPEVDKKTNEQYLSIGAKKSGNKTERDEDRRVSDNLHKMEKVKFPKGMPAKYFLANES
                     AEDIKMRKKIFPKLKSWEQQHIDLSEDPKDVSVETLDASHLIYHTQYKHMAKAIDEWI
                     QSHEK"
     gene            4307..5368
                     /locus_tag="VBD27_001346"
     CDS             4307..5368
                     /locus_tag="VBD27_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7025355.1"
                     /translation="MNKITTLSTIALTLMLCVGCSANKDNQKTKTEDPKQENVTANPK
                     EDKATAAKRMKLTKELEKYNKGTESFISNKQYQKYIKTPIKEYGQKVEVKGKKMNVYT
                     VGEGKVPIVFIPGQGTVTAKHQYHNLISNLSKTHKVVVVEPFGSGLSDVIDQPRNLAN
                     ITSDIHEALQKVGITGKYVIASHSIGGVYALKYISTYPKEVLGLIGLDTSTPGMEGGK
                     QVDFAAPVLKELPKIPKVSDDINAQFFAIGHKILNNSNMKEEAKNSSNMINESANYKI
                     PKGIPAMYLLARESDLGLQLRSLDIPIKGSWQEQHKELSEDPKEVSIHTLDGTHQIYD
                     TNLKEVIDYTNQFMKTFEK"
     gene            5492..6721
                     /locus_tag="VBD27_001347"
     CDS             5492..6721
                     /locus_tag="VBD27_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025356.1"
                     /translation="MNLWTLSLAYLKRQKMKTVTLFLVFLTIGTCLISLMSIQHSLEK
                     NILTKQGKSIYLTSKEKAYWPEQAYEALKKAKMVESVEASLSIDVGSRLKSVSPYNSS
                     KEENQVTLAGYQSTEDLRAFQTKALVLKKGSHLAADNTKQVLVPLKLAQKNHLSVGNK
                     LRLGKENVTIAGIYDANSAKSKNTFNPNIDNTLIAQATLVRKISKQKGYQTVAVRLSD
                     KRLVDTVIQNIKQWPLDFGKLDVQTAKEFYGDSYRNIETLHRLVGRIILIVSLVAMAI
                     LVVMLTFWINNRIKETGILLAIGKTKFEIIGHYLIEVLLVAGAAFTLSIIGGVFLGKT
                     FAAGLLSQVNGGVSSQIVQNSSLIIDRIDNLAVSVGVMDVFRLYAQGALICLFAVVLS
                     SYSILKLQPKQILSRMS"
     gene            6731..7525
                     /locus_tag="VBD27_001348"
     CDS             6731..7525
                     /locus_tag="VBD27_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025357.1"
                     /translation="MNLFKRSFLYVSRKKRKSITLFVCLWLVASTLISGIAVKNAGLT
                     AKKTFSRQTGSILHISSDSTDLVGDGYGSGEIPEKAIVNIASNPNVKRVNNNLMAYAG
                     LTSEKMVTRPNDKEQYKEQVLQVHGNSYSDTDPKYTAGMISLKGGRHITPKDQRVIIV
                     HQAFAKTNHLKVGDKLMFSKDPVRNSKDKTLVEATIVGIYKGKTVQKSNYPYEMLENT
                     AFSDTQLIKDLYGYSKGKAFYRQTTVYPKKNADIETLKSFIKKQSY"
     gene            7626..7754
                     /locus_tag="VBD27_001349"
     CDS             7626..7754
                     /locus_tag="VBD27_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7025358.1"
                     /translation="MLVVSIILVMMALLFWIGGRTHETGILLAIGKSKAIIIVSTQ"
     gene            7763..8083
                     /locus_tag="VBD27_001350"
     CDS             7763..8083
                     /locus_tag="VBD27_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7025359.1"
                     /translation="MIAIPAFALSVLISQFIGQKLADSLVYQASRTVRADFMKGFGNM
                     QLGADSDTDMFMQTIKTLNVQVKPEEVWLVILVGLAIVLFAIITTSLIIARYKPRGIL
                     SKLS"
     gene            8180..>8813
                     /locus_tag="VBD27_001351"
     CDS             8180..>8813
                     /locus_tag="VBD27_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025360.1"
                     /translation="MLELKNIAYRYKGNDNKTLENINYSFQSGVFYTILGNSGSGKTT
                     LLSLMAGLDSPTEGQVLFNKKDIKEAGYAQHRKKNIALVFQNYNLLDYLTPLENVQLV
                     KPTADKQLLLDLGLKEDMLTRNILRLSGGQQQRVAIARALVVGTPAILLDEPTGNLDF
                     DIARDITMRLKDFAHKEKRCVIMVTHSREIAHMADTALQLIGDNLKELSKE"
BASE COUNT         3245 a         1372 c         1682 g         2514 t
ORIGIN      
        1 aatcaaaagt agtagctcat tagctactac ttttttgtat aagtgtaaaa aagtgaattc
       61 ttaacaagaa tttaacaccc tacttgctat actaaaagtg aataagaaag agaggtgtca
      121 tttatgcaaa gaaaatccta tttaaaatcc atgagtgttc ttactttaac agcttgtctt
      181 atatcaggat atgtggttaa agatattgct atgttacatg cagtatctgc cagtgagaag
      241 aaagcaaata atgtcagtcc gagagaaaat ctctacaggg ctgtcaatga taattggcta
      301 gccaatacaa aactcaaaca agggcagact agtgttaata gtttttcaga aattgaggat
      361 aaattaaagc aactgttagt gtctgatatg gctaaaatgg cctcaggaaa gattgaaaca
      421 accaatgatg aacagaaaaa aatggttgca tactataaac aaggtatgga ctttaaaaca
      481 agagataaaa atggtctcaa acctctaaaa ccagttttac aaaaacttga agcagtctct
      541 tcaatgaaag actttcaaag tttggcccat gattttgtga tgagtggttt tgttttacca
      601 tttggtttga ctgtggaaac caatgctcga gataatagcc aaaagcaatt ggtgcttcgt
      661 caagcacccg cattacttga atcacctgac caatataaga agggcaataa agaaggtgag
      721 gctaaattat cagcttaccg tacttcagca atggctttgc ttaaacaagc tggaaaaagt
      781 aacattgaag atagaaaact agttaaacaa gctatagcat ttgatagact cttatcagaa
      841 aaaacgcaag ttgatcaaag taaaatcaca gctgaaagtg agacagctgc ggggcgatat
      901 aaccctgaaa gtatggaaac ggttcacaat tacgccaagg aatttgactt taaagaattg
      961 attgaaaaac tagttgggcc aacgaataag gcagtcaatg tagaagataa aacttatttt
     1021 aaacaggtta atgatgttat aaatagtaaa caattagcca atatgaaagc atggatgatg
     1081 atttctatgc tagttgatca atcagatttt ctaggagaac aaaatcgtca agcagcgagt
     1141 gcttttaaga atgttgcgtc tggtttgact cagattgaat cgaaagaaaa aatgcttaca
     1201 cccaattaga atatactttt ggccatattt tgggtgatta ttatggtaag aaatattttg
     1261 gtgaggcagc taaaaaagac gtcgaacata tggctaagaa aatcattaat gtctataaaa
     1321 cacggttaaa aaacaacact tggttatcag aaaatacaaa agcaatggcc attaagaaac
     1381 ttgataacat gagattaatg attggctatc cagaagatta tcctgatctt tatcgtcagt
     1441 accaatttga tagtaaagca agcttctttg aaaacaatga taactacaga aaattatcga
     1501 acaagaaaac atttgaagaa tttaaccagt ctaatcaacg tgaacattgg caaatgagtg
     1561 ccaatgctgt aaatgcttat aatgatccta ataccaattc catagtcttt ccagcagcga
     1621 tttttcaatc accactctac gataaaacta aaacagttag tcaaaattat ggagctatcg
     1681 gagcaattat tggtcatgaa atttcacact catttgatat taatggtatg aaatatgacg
     1741 agaaagggaa tcttcacgat tggtggacta aagaagattt aaaacattat aagaaaaaaa
     1801 cacaagctat gattgaccaa tgggatggcc ttaaagcaga tggcggtaaa gttgatggta
     1861 aattaacttt agcagaaaat attgcagata atggtggtgt tatggcatct ctagaagctc
     1921 ttaagactga aaaaatccaa actataaaga attttttgaa tcatgggcaa gtatttggcg
     1981 tcaaaaagca accaaagaac aaagtaagtc ctcaattcag tcagatgttc atgcaccata
     2041 tgaattgaga gctaacatcc cagtacgtaa tttccaagaa ttttatgatg cctttggtgt
     2101 taaaaaaggc gattcaatgt atctaaaacc agaaaaacgt ttgacacttt ggtaaagcta
     2161 tgttttccag acaataagga gaatgcatga agaatataac aaagctatca actgttgctt
     2221 taagcctact actttgtacg gcgtgtgctg catcaaacac gtctacatct aaaacacagt
     2281 ctcatcatcc taaacaaact aaactcacag ataagcaaaa agaagaaccc aaaaacaaag
     2341 aagctgctga tcaagagatg catccccaag gcgctgttga tttgacaaaa tataaggcaa
     2401 aaccggtcaa agattatggt aaaaaaatcg atgttggtga tggcaagaaa atgaacattt
     2461 atgaaactgg tcagggaaaa attccaattg tttttattcc tggtcaagct gagatttcgc
     2521 cacgctatgc ttataagaat ttaattgagc ggttaagtaa aaaatataaa atttatacag
     2581 tagaaccatt aggctatggt ctcagtgata ttcctactaa accacgtacg ctagaaaaca
     2641 ttacaaaaga aattcacaca ggcctaaata aaattggagt taaaaatttc taccttgcag
     2701 ctcactcact tggcggtatg tattctttaa attatgctaa aaattatcca gaagaagtca
     2761 gaggatttat tggtatggat accagtacac cttggatgga aggggaacaa aaaacaaagt
     2821 atgaccctga aagtgctaaa tgggcaatga aattaccaga tgttgatgat aagactaacg
     2881 aacagtatct gtcaattgcg aaaaaaatta acggcaataa gtctcaacga gatgaagatg
     2941 agcgcttagg tgataatctt ggtaaattga agaatgtaaa attccctaaa gggatgccaa
     3001 ctaagtattt tcttgctgat caatctgtgg agggaatcaa gctacgtcaa gcagatttta
     3061 aaggtttgaa aaattgggat cagcagcata ttgatttatc tgaagatcca aaagatgctt
     3121 ccgttgaaat tcttaaaggt agtcacaata ttttccaaac acaatatgaa catatggcta
     3181 aagctattga tggatttatc caatcccatc aaaagtaatc gttgaatgaa atacctaagt
     3241 caaggagaaa aaatgaataa aataacgaaa ttatcaaccg ttgccctcgc tttgatgctt
     3301 tgcgttggat gttctgccaa taaagataat caaaaaacta aaactgagca gcctaagcaa
     3361 gaaaagtcaa tcgctaagca aaaacaagag agagaagcag aaaaagctat gattgctaaa
     3421 ggtgctattg acctaactaa atataaagcg aaaccaattg aagattacgg acaaaaaatt
     3481 gatgtcggtg acggtaaaaa aatgaacata tatacaatgg gtcaagggaa aattccgcta
     3541 gtagtcttcc ctggtcaagc tgaaatttca ccacgttatg cttacaaaaa cttgcttgat
     3601 agattaggga aaaaatataa aatttatacg gttgaaccgc tcggttatgg gcttagtgat
     3661 atgacagata agccacgtac gattgaaaat attaccaaag agattcatac agctcttgaa
     3721 aaagtcggtg tcaaaaattt ctatcttatg gctcactcgt taggtggcat atacggtcta
     3781 aattatgcta agacatattc taacgatgtc aaaggtttca ttggcatgga tacaagcaca
     3841 ccaaatatgg aaggtgatgg cacaactgga gaatcttcag cttcaacctt aaaatgggca
     3901 gaatccattc cagaagttga taagaaaacc aatgaacaat acttatcaat cggtgctaaa
     3961 aaatcaggta ataaaacaga gcgcgatgaa gataggcgtg ttagtgacaa tcttcataaa
     4021 atggaaaaag ttaagttccc taaaggtatg ccagctaagt atttcttagc taatgagtca
     4081 gcagaagata taaaaatgcg taaaaaaata ttccctaagc ttaaaagctg ggaacaacaa
     4141 catattgact tatctgaaga tccaaaagac gtttctgttg agacactaga tgctagccac
     4201 ttgatttatc atacgcaata taaacatatg gcgaaggcta ttgacgagtg gattcaatcg
     4261 catgaaaaat aattattccc cccaattatt ttactaagga gaaaagatga ataaaataac
     4321 gacattatca accatcgccc tgactttaat gctttgcgtt ggatgttctg ccaataaaga
     4381 taatcaaaaa actaaaactg aggatcctaa gcaagaaaac gtaactgcaa atccgaagga
     4441 agacaaggca acagcagcca agcgaatgaa gttaacgaaa gaacttgaaa aatataataa
     4501 aggcacagag agctttatta gtaacaaaca atatcaaaaa tatatcaaaa cacctatcaa
     4561 agaatatggt caaaaggttg aagtcaaagg caagaagatg aatgtctata ctgttggaga
     4621 aggtaaagta ccaattgtct ttattcctgg tcaagggacg gtgactgcca aacatcagta
     4681 ccataatttg attagtaatt taagtaaaac tcataaggtt gtggtagttg agccatttgg
     4741 ttcaggttta agtgatgtca ttgaccaacc acgaaatcta gctaatatta cctcagatat
     4801 ccacgaagct ttacaaaaag ttggtattac cggaaaatat gtaattgcct ctcattcaat
     4861 tggtggtgtt tatgccctta aatacatcag tacttatcca aaggaagtgc taggattaat
     4921 aggtttagat acatcaacgc cgggtatgga aggtggtaag caagttgatt tcgctgctcc
     4981 agtgttaaaa gaattaccta aaattcctaa agtatccgac gatattaatg cacaattttt
     5041 tgctattgga cataagatac ttaataattc taatatgaaa gaagaagcga agaattcttc
     5101 taatatgatt aacgaaagtg ccaactataa gattccaaag ggaattcctg ctatgtattt
     5161 attagcacgt gaaagtgact tagggcttca attacgtagt ttagatatac caattaaagg
     5221 cagttggcaa gagcaacata aggagttatc agaagatcca aaagaagtct ctattcatac
     5281 actagacgga actcaccaaa tttatgatac taaccttaaa gaagtaattg attatactaa
     5341 ccaatttatg aaaacatttg agaaataatt gtttttagaa acagtgctat aaaatcacta
     5401 attgatagat aacgtagaag tcgatagaga atatctaacg acttctatcg ttattctaga
     5461 gattattaag atagaaaaag gagggaaaat agtgaaccta tggacattaa gtttagctta
     5521 tttgaaacgt caaaaaatga aaacagtaac cttgttctta gtttttctaa ctataggcac
     5581 atgtttaatc agtctgatga gtattcagca ttctttggaa aaaaatatcc taacaaaaca
     5641 agggaaaagc atttatttaa cctccaaaga aaaggcttat tggcctgaac aagcatacga
     5701 agctttgaaa aaagcaaaaa tggttgagtc agtagaggca agtttaagca ttgatgttgg
     5761 tagccgttta aaatctgtat caccatataa ctcttctaaa gaagagaatc aagttacatt
     5821 agcaggttat caaagtacag aagatttacg agcttttcag acaaaagcat tggtcttaaa
     5881 gaaaggaagc catttagcgg ccgataatac aaaacaagta ctagttccat tgaagttagc
     5941 acaaaagaat catttaagtg tgggtaacaa attgagatta ggcaaagaaa atgttactat
     6001 tgcaggaatt tatgatgcga atagtgctaa gtctaaaaat acttttaatc ccaatattga
     6061 taatacattg attgctcagg caacattggt taggaagatt agtaaacaaa aaggttatca
     6121 gacggttgca gtgcgcttaa gtgataaacg tttagtggat acagttatcc aaaacatcaa
     6181 acaatggccg cttgatttcg ggaaattaga tgttcaaaca gctaaagagt tttatggaga
     6241 ttcttatcgt aatatcgaga cattgcatcg tctagtggga cgaatcattc ttattgttag
     6301 tctcgtggcg atggcaatat tagttgtcat gctaacattt tggattaata accgcattaa
     6361 agagacggga attttactcg ctatcggaaa aactaagttt gaaatcatag gtcattatct
     6421 gatagaagta ctgttagtag caggtgctgc cttcacacta tctattattg gtggtgtctt
     6481 tcttggaaaa acatttgcag ctggcctttt atcacaagtt aatggtggtg tgagttcaca
     6541 aattgtacaa aatagtagtc taattattga tagaattgat aacctcgctg tttctgttgg
     6601 agtaatggat gtctttagac tctatgctca gggtgcttta atttgcttat ttgcagttgt
     6661 attatctagt tattctattc ttaaactaca accaaagcaa atattgtcac gaatgtcata
     6721 ggaggttaat atgaatttat ttaaacgttc ctttttatat gttagccgta aaaagcgtaa
     6781 gagtatcact ttattcgtat gtttatggct agttgcttct acattaatta gcggtattgc
     6841 ggtaaaaaat gctggtctta ctgctaagaa gacattttca aggcaaactg gatcaatttt
     6901 gcatatttca tcggatagta ctgaccttgt tggtgatggt tacggatctg gagaaatccc
     6961 tgaaaaagca atagttaaca tagcaagtaa tccaaatgtt aagcgcgtta ataataattt
     7021 gatggcttat gctggactaa cctcggaaaa aatggttaca cgaccaaatg ataaagaaca
     7081 atataaagaa caagttttac aagttcatgg taatagttac tccgatacag atcctaaata
     7141 cacagcgggg atgatttctc ttaagggagg gcgacatatc acgccgaaag atcagcgagt
     7201 tataattgtt catcaggctt ttgcgaagac aaatcatctt aaagttggcg ataagttaat
     7261 gttttcaaaa gaccccgtta gaaacagtaa ggataaaaca ttagttgaag caactattgt
     7321 tggcatatat aaaggtaaaa cggtacaaaa atcaaattac ccttatgaga tgctagaaaa
     7381 tacagccttt tcagatacgc aattgattaa ggatttatac ggctattcta aaggtaaggc
     7441 cttttatcgc caaacaactg tatatcctaa aaagaatgct gatatagaaa ctcttaaaag
     7501 ttttatcaaa aaacaatcct attgattgga ataaatatca actgacaatt acagcaacct
     7561 tgctaacaag ttatgcaaaa agtatagatg tcttgaatca attaattact tctctacaag
     7621 gcggcatgtt agtagtatct attatcctag tgatgatggc tctgctattc tggattggtg
     7681 gtagaaccca tgagacaggt attttattag ctattggtaa gtctaaagca attatcattg
     7741 tcagtacgca gtagaagtac taatgattgc tattccagcc tttgctttat cagtacttat
     7801 cagtcaattt attggtcaaa aattagcaga ctccttagtg tatcaggcct ctagaactgt
     7861 cagagcagac tttatgaaag ggtttgggaa tatgcagctt ggggctgata gtgatacaga
     7921 tatgtttatg caaacgatta agacgttaaa tgtacaagtg aagcctgagg aagtctggtt
     7981 agtaattcta gttggtttag caatcgtctt atttgctatt attactacta gtttaattat
     8041 tgctcgctac aaaccaaggg gaatcttatc aaaactgtcc taaagtgcca taagtatacc
     8101 gaccaatagt gacatctata ttaacggtta aaatgaatat gctctgctat tgaatacata
     8161 gctcaagaaa gagaaatcta tgctcgaatt aaaaaatatt gcttatcgtt ataaggggaa
     8221 cgacaataaa acattagaaa atatcaatta tagttttcag tcaggggtat tttatactat
     8281 attagggaat tctggttcag ggaaaacaac ccttctatcc ttgatggcag gtcttgattc
     8341 gcctactgag ggacaagtat tatttaacaa aaaagatatt aaagaagcag gttatgctca
     8401 gcatcgcaaa aagaatatag ctttggtctt tcaaaattat aaccttttag actatctaac
     8461 cccattggaa aatgttcagt tggtaaaacc tactgcagat aaacaattat tgttggattt
     8521 aggtttaaaa gaagatatgt taacgcgaaa tatcttgaga ttatcaggag ggcaacaaca
     8581 acgtgtagct attgcgagag ctttggttgt aggaacacca gctattttac ttgatgaacc
     8641 tacgggaaat ttagattttg acattgcgcg tgatattacg atgcgtctta aagattttgc
     8701 gcataaggaa aaacgatgtg ttattatggt gacacatagt agagagatcg ctcatatggc
     8761 tgatactgct ttacaattga taggagataa cttaaaagaa ttatcaaaag aaa
//
LOCUS       DATRQA010000078         8749 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.078, whole genome shotgun sequence.
ACCESSION   DATRQA010000078 DATRQA010000000
VERSION     DATRQA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.078"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            9..1379
                     /locus_tag="VBD27_001352"
     CDS             9..1379
                     /locus_tag="VBD27_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO7025361.1"
                     /translation="MSKIVVVGTNHAGTAAIKTMLSNYGEANEIVTFDQNSNISFLGC
                     GMALWIGEQIDGPEGLFYSDKEQLESMGAKVYMNSPVLNIDYDKKEVTALVDGKEHVE
                     SYEKLILATGSQPIIPPIKGVEIQEGSREFKATLENLQFVKLYQNSEEVIEKLAKPGI
                     NRVAVVGAGYIGVELAEAFQRIGKEVTLVDVADTCMGGYYDRDFTDMMSKNLEDHGIR
                     LAFGQAVQAVEGDGKVERLVTDKETFDVDMVILAVGFRPNTELGAGKLDTFRNGAWVV
                     DKKQETSVKDVYAIGDCATIWDNSRDDINYIALASNAVRTGIVAAHNACGTELEGAGV
                     QGSNGISIYGLNMVSTGLTLEKAKQAGYNAVETGFNDLQKPEFIKHNNHEVAIKIVYD
                     KDSRVILGCQMVSHEDVSMGIHMFSLAIQEKVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(1500..2489)
                     /locus_tag="VBD27_001353"
     CDS             complement(1500..2489)
                     /locus_tag="VBD27_001353"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO7025362.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALF
                     DKAVGDAEDLSHALAFTSPKKIYAATYADCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLF
                     ISVRDAAYSIINKKGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFI
                     GQPAIVGAHGIVRPVNIPLNDAELQKMQASAEQLKDIIDEAWKNPEFQEASKN"
     gene            2728..5187
                     /gene="gyrA"
                     /locus_tag="VBD27_001354"
     CDS             2728..5187
                     /gene="gyrA"
                     /locus_tag="VBD27_001354"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO7025363.1"
                     /translation="MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRIEGITAVRDESSREGVRFVIEVRRDASANVILNNLFKLTSLQTNFSFNMLAI
                     EKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAEARAHILEGLLVALDHLDEVIT
                     IIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIAD
                     LADILAKPERVVTIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGIQGTGVNNDDFVRELVSTSTHDTVLFFTNLGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQQGIVKRTS
                     VSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRT
                     ATGVRGVNLREGDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTAN
                     ITAKNGPLARLVTINGNEDIMVITDTGVIIRTNVANISQTGRSTMGVKVMRLDQEAKI
                     VTVALVEQEIEDKSNIEDTKE"
     gene            5194..5937
                     /locus_tag="VBD27_001355"
     CDS             5194..5937
                     /locus_tag="VBD27_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO7025364.1"
                     /translation="MRNKKKSHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQS
                     NHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVE
                     INLPIFKGLGNTELSYGAGTMKENQIMGGPNNYALASHHVFGLTGSSKMLFSPLEHAK
                     KGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVH
                     AELEKTGEFSTADESILKAFSKKYNQINL"
     gene            5953..6366
                     /locus_tag="VBD27_001356"
     CDS             5953..6366
                     /locus_tag="VBD27_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7025365.1"
                     /translation="MKLKAVHHIAIIVSDYEKSKDFYVNKLGFEIIRENHRPERHDYK
                     LDLRCGDIELEIFGNRLDDPEYETPPQRIGRPNWPREACGLRHLAFYVPDVEAYKVEL
                     ENLGIFVEPIRYDDYTGKKMTFFFDPDGLPLELHE"
     gene            6519..7481
                     /locus_tag="VBD27_001357"
     CDS             6519..7481
                     /locus_tag="VBD27_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEO7025366.1"
                     /translation="MSIKKLLFAGLAFILFTLASPAYAASDVQKVIDETYVQPDYVLG
                     YSLNQEQRAQTLQLLNYDESRDTKVKTLNTSSYAKIMNIADDASIQLYSSVKIKKLGS
                     NDTLAVNIVTPENITKVTEDMYRNAAVTLGIEHATISVAAPIKVTGESALAGIYYSLE
                     KNGASVSSENKQLAQEELSTLSGINAENKGKEGYDADKLNVALTDIKSAVAKGGSDLS
                     KDDIRKIVEETLKNYHLDNAVTENQINLIVNFAVNLSQSNVIKNSDFTNTLNNLKDNI
                     VSKAGSKFKNINVNFNANKAVESGKGFLANIWQQIVNFFQQFGS"
     gene            complement(7544..8671)
                     /locus_tag="VBD27_001358"
     CDS             complement(7544..8671)
                     /locus_tag="VBD27_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO7025367.1"
                     /translation="MHIIIQITIILLASVLATLISKRIGIPAVVGQLLVGIIIGPAML
                     GLVHQNQVLHVLSEIGVILLMFLAGLEANFDLLKKYLKPSLLVAITGVIVPMALFYFL
                     TRLFGFQINTAIFYGLVFAATSISITVEVLQEYNRVKTDTGAIILGAAVADDVLAVLL
                     LSVFIATNGSSSNIGLQIIIQLLFFVFLFICMKYLVPALFKLIEKVHFFEKYTILAIL
                     ICFSLSILADKVGMSSIIGSFFAGLAIGQTSFVDKVEHKISLLSYTFFIPIFFASIAL
                     PLKFDGMMSHLHTILIFTALAVLSKLIPGYFVGRGFNFSKLESLTIGGGMVSRGEMAL
                     IIVQVGLAAKIISSTTYSELVIVVILSTIIAPFILKYSFKD"
BASE COUNT         2978 a         1394 c         1728 g         2649 t
ORIGIN      
        1 ctcttaatat gagtaaaatc gttgtcgtag ggacaaacca cgctggtaca gcagcaatta
       61 aaacaatgct tagtaattat ggtgaagcca atgaaattgt aacatttgat caaaattcca
      121 atatctcttt cttaggatgt ggtatggctc tttggattgg tgaacagatt gatggtccag
      181 aaggattgtt ctactctgat aaagagcagt tggaaagtat gggagctaag gtttacatga
      241 actctccagt acttaatatt gactacgata aaaaagaagt tacagcttta gtagatggaa
      301 aagaacatgt tgaaagttat gaaaaactta ttttagcaac tggttcacaa cctatcattc
      361 caccaattaa aggtgttgaa attcaagaag gatctcgtga atttaaagct actcttgaaa
      421 accttcaatt tgtgaaactt tatcaaaatt cagaagaagt tattgaaaag ttagctaaac
      481 ctggtatcaa tcgtgttgct gttgttgggg ctgggtatat tggtgtagaa ttagcagaag
      541 ctttccaacg tattggaaaa gaagtaacac ttgttgatgt tgctgatact tgtatgggtg
      601 gttactatga tcgtgacttt actgatatga tgagtaaaaa ccttgaagac catggtattc
      661 gtcttgcttt cggacaagct gttcaagcag ttgaaggtga tggtaaagta gaacgtcttg
      721 ttactgataa agaaacattt gatgttgata tggtgatctt agctgttgga ttccgtccaa
      781 atacagaact cggtgcaggt aaattagata cattccgtaa tggcgcttgg gtagttgata
      841 agaagcaaga aacaagcgtg aaagatgttt atgctatagg tgattgtgcg acaatttggg
      901 ataattctcg tgatgatatc aattatattg ctcttgcttc aaacgccgtt cgtactggta
      961 ttgtagcagc tcacaatgca tgtggtacag agcttgaagg tgctggtgtc caagggtcaa
     1021 atggtatctc aatctatggc cttaacatgg tgtcaactgg tttgacttta gaaaaagcaa
     1081 aacaagctgg gtacaatgca gttgaaacag gctttaatga ccttcaaaaa ccagaattta
     1141 tcaaacacaa taatcatgaa gtagctatta agattgtcta tgataaagat agccgagtta
     1201 tccttggttg ccaaatggtc tcacacgaag atgtatctat gggaattcat atgttctcac
     1261 ttgctattca agaaaaagtt actattgaaa agcttgcttt gaccgatatc ttcttcttac
     1321 cacattttaa taagccatat aactatatta caatggctgc attaggcgct aaagattaat
     1381 ttaaaattaa aaaggttaag cgaaatgctt agccttttat ttcatccttt aacaaggtca
     1441 aaaatgtaac ggcaaagaaa aagctaagat attcctatcc tagctttttt tcaactatgt
     1501 tagttttttg atgcttcttg gaattctggg tttttccaag cttcgtcaat aatatctttt
     1561 aattgttcag ctgaggcttg cattttttga agttcagcat catttaatgg gatattaact
     1621 ggacgaacga taccatgtgc acctacgatt gcaggctgac cgataaagac atctttaaca
     1681 tcaccgtatt ggccttcttg atatacagat aatggaagaa ctgcattttc atcatcaagg
     1741 atagctttag taatacgagc aagtgcgaca gcaataccgt aatatgtagc accttttttg
     1801 ttgatgattg agtatgcagc gtcacgaact gaaataaaca aatcaacaag tccttgttca
     1861 tcaatatcgc gattttcttg taaccattgt tcaagttgga caccagcaac gttagcatgt
     1921 gaccaaacgg caaattctga gtcaccgtgt tcacccatga tgtatgcgtg aactgaacga
     1981 gcatcaacac caattttatc tgctaaagct tgacggaaac gtgcagagtc aagtgaagta
     2041 cctgaaccaa taacgcgttc cttagggaaa cctgagaatt tccaagttga atatgtaagt
     2101 acatctacag ggttagccgc tactaagaag ataccgttga aacctgattc aacaacttga
     2161 gttacgattg atttgttaat tgctaagttt ttaccaacaa ggtcaagtcg agtttcacct
     2221 ggtttttgag gtgcgccagc agtaatgaca acaaggtcag catctgcgca gtctgcataa
     2281 gtagctgcgt agattttttt aggtgatgta aatgcaaggg catgtgaaag atcttcagca
     2341 tcaccaacag ctttatcaaa taaagctggg atttcaatga tacctaactc ttgcgcaata
     2401 ccttggttaa caagtgcaaa agcgtaagaa gaacctacgg caccatcacc aacgagaata
     2461 acttttttat gttgtttagt tgcagtcatt tctaaacatc tccttaaata tttttgggaa
     2521 gtttcccata cacctatatt ctatcatttt caatgaaaga agtcactaat aaaatgtgaa
     2581 aaaatttgca aacaaattga aatatcatag aaaactattg aaaacgctga gtgtcttgat
     2641 atttttgata ccgatttatc atcgcaattt tacctaaatt atggtacaat gtaagagact
     2701 tacagtgaag aaaaggggca ttttttaatg caagataaaa atttagtaga tgttaatcta
     2761 actagtgaaa tgaaaacgag ttttatcgat tacgccatga gtgtcattgt tgctcgtgca
     2821 cttccagatg ttagagatgg tttaaaacct gttcatcgtc gtattttata tggtatgaat
     2881 gaactaggtg tgacacctga taaacctcat aagaaatcag cacgtattac tggtgatgtt
     2941 atgggtaaat accatccaca tggtgattca tctatttacg aagcaatggt gcgtatggca
     3001 caatggtggt catatcgtca tatgctcgtt gatggtcatg gaaactttgg ttcaatggat
     3061 ggggatggtg ctgccgcaca gcgttataca gaagcacgta tgagtaaaat tgctcttgag
     3121 atgcttcgtg atatcaataa aaatacagtt gattttcaag ataactatga tggcagtgaa
     3181 cgtgaacctc ttgttttacc agcgcgtttt cctaatttat tagtcaatgg tgcaactggt
     3241 attgctgtag gtatggcaac aaatattcca ccacataatc taggtgagtc tatagatgct
     3301 gtcaaattgg ttatggataa ccctgatgtt actacacggg agttaatgga agttattcct
     3361 ggtccagatt ttcctactgg tgccttggtg atgggacgtt caggtattca ccgtgcctat
     3421 gaaactggga agggatcaat tgtcttacgt tcacggactg aaattgaaac aacttcaaat
     3481 gggaaagagc gtattgttgt cacagagttt ccatatggcg ttaataaaac aaaagtacac
     3541 gagcatattg tacgtttagc acaagaaaag cgcattgaag gtattactgc tgttcgtgat
     3601 gaatcaagtc gtgaaggcgt tcgttttgtt attgaagttc gtcgtgatgc ttcagcaaat
     3661 gtgattttaa ataatctttt caaattaact agcttgcaga caaattttag cttcaatatg
     3721 cttgctattg aaaaaggtgt tccaaaaatt ttgtcattac gacaaatcat tgataactat
     3781 attgaacacc aaaaggaagt tattgttcgt cgtactcagt ttgacaaagc taaagcagaa
     3841 gcccgtgctc atatcttaga aggtttactt gtggcgcttg atcacttgga tgaagttatc
     3901 acaattattc gtaatagtga gacagacacc attgctcaag cagagttgat gtcacgtttt
     3961 gaattatcgg agcgtcagag tcaagctatt ttagatatgc gtcttcgtcg tttaactgga
     4021 ttagaacgcg ataaaatcca atcagaatac aatgacttac tagctttaat tgctgattta
     4081 gcagatatct tagctaagcc tgaacgtgtt gttaccatta taaaagaaga gatggatgag
     4141 gttaagcgta agtatgctga tgcccgtcgt acagaattaa tgattggtga agttttatca
     4201 cttgaagatg aagaccttat tgaggaagaa gatgttctta ttactctctc aaacaaggga
     4261 tatatcaagc gtctcgctca agatgagttt agagcacaaa aacgtggagg acgcggtatt
     4321 caagggactg gagttaataa cgatgatttt gttcgtgaat tggtttcaac aagtactcac
     4381 gatactgtgc ttttctttac aaatttaggg cgtgtgtatc gtttgaaagc atatgaaatt
     4441 ccagaatatg gacgtacagc taaaggcctc cctatcgtta accttttaaa acttgacgaa
     4501 ggtgagacaa ttcaaacaat tattaacgct agaaaagaag atgtcgctaa taaatatttc
     4561 tttttcacaa ctcagcaagg tatagtgaaa cgtacaagtg tttctgaatt tagtaacatt
     4621 cgtcaaaatg gacttcgtgc tattaatttg aaagagaatg acgagttgat taacgtcctc
     4681 ttgattgacg aaaatgaaga tgttattatc gggacacgta caggctactc tgttcgcttc
     4741 aaagtgaacg ctgtacgtaa tatgggacgc acagcgactg gggtacgagg cgttaatctt
     4801 cgtgaaggtg ataaagttgt gggagcttct cgtatcgtta atggacaaga agtactaatc
     4861 atcactgaaa aaggttatgg caagagaaca gaagcttcgg aatatcctac aaaaggccgt
     4921 ggtggtaaag gaataaaaac cgctaatatt actgctaaaa atgggccatt agcacgctta
     4981 gtgacgatta atggaaatga ggacatcatg gtcattacag atacaggtgt tattattcga
     5041 accaatgttg ctaatatttc tcaaactggt cgttctacca tgggtgttaa agtgatgaga
     5101 cttgatcaag aagctaaaat cgtcactgtt gcgctagtgg aacaagaaat tgaagataag
     5161 tctaatatag aggatacaaa agaataggaa gttatgcgta ataaaaaaaa atcacatggt
     5221 ttctttaact ttgttagatg gttattggtt gtattgttaa ttattgttgg gttggctttg
     5281 gtgttcaata aaccaattcg caatgctttc atagctcatc aatcaaatca ttatcaaatt
     5341 tcacgagtta gtaaaaaaac aattgaaaaa aataagaaat ctaaaacaag ttatgacttt
     5401 tcatcagtca aatctattag tacagaatcg attttatctg ctcaaacgaa atcacataat
     5461 ttgcctgtaa taggagggat tgctatacca gatgtcgaaa tcaatctccc aattttcaaa
     5521 ggactgggca atacagagtt gagttatggt gcaggaacga tgaaagaaaa ccagattatg
     5581 ggaggcccaa ataactatgc cttagctagt caccatgtct ttggtttgac aggttcttct
     5641 aagatgctct tttcaccttt agaacatgct aagaaaggta tgaaagttta cctcactgat
     5701 aaaagcaaag tatacactta cactattact gaaatttcaa aggtaacacc agaacatgtt
     5761 gaggttattg atgatacacc tggcaagagc caactcactt tagtgacctg tacggatcca
     5821 gaagccacag aacgtattat tgtgcatgct gaattagaaa aaacaggtga attttcaaca
     5881 gcagatgagt cgatattaaa agcattttcg aaaaaatata atcaaattaa tctctagaag
     5941 gtagttagaa ttatgaaatt aaaggctgtt catcatattg cgataattgt ttctgactat
     6001 gaaaagtcaa aagacttcta tgttaataag ctaggatttg aaattattcg tgaaaaccat
     6061 cgtccggagc gtcatgatta taaattagat ttacggtgcg gtgatattga gcttgaaata
     6121 tttggaaatc gtcttgatga tccagaatat gaaactcctc ctcagagaat aggtaggccg
     6181 aattggccac gtgaagcatg tggtttacga caccttgcct tttacgttcc agatgtggaa
     6241 gcgtacaagg tagaactgga gaatttagga atttttgtag agcctattcg ttacgatgac
     6301 tatactggca aaaagatgac ctttttcttt gatccagatg gtttaccttt agaattacat
     6361 gaataataaa caaagttgcg tggatacgca actttgttta atttactgaa taattttata
     6421 ttcaagaagt tttttaaaga aataagtaag ttaatcatgg tataatgtaa ggtatgaaaa
     6481 gaattgaaaa ggaattattt tgaaatgagg atactaaaat gagtattaaa aagctattgt
     6541 ttgcaggcct tgcttttatt ttatttaccc tagcgagtcc agcatatgct gcatcagatg
     6601 ttcagaaagt tattgatgaa acttatgttc aacctgatta tgtcttaggt tattcgctaa
     6661 atcaagaaca gcgtgcacag acattacaac ttctgaatta tgatgaatct agagacacga
     6721 aagttaaaac gctaaatact agctcatatg ctaaaataat gaatatagct gatgatgcaa
     6781 gcatccaatt atattcatct gttaaaataa aaaaattagg gtctaacgat accttagcag
     6841 taaacattgt tacacctgag aatattacta aagttacaga agatatgtat cgcaatgctg
     6901 cagttacatt aggtattgag catgcaacga tatcagtggc tgctccaata aaagtaactg
     6961 gggagagtgc tcttgcaggt atctattatt cattagagaa aaatggagca agtgtatcat
     7021 cagaaaataa acaactagca caagaagagt taagtacttt atctggtatt aatgctgaga
     7081 ataagggtaa ggaaggttat gacgccgaca agcttaatgt agctttaacg gatattaagt
     7141 cagccgtagc taaaggagga tctgatcttt caaaagatga tattcgaaaa attgttgagg
     7201 aaacattgaa aaattatcat cttgataacg ctgtaacgga aaatcaaata aatttaattg
     7261 ttaattttgc agttaatctt tcacaaagta atgtcatcaa aaatagtgat ttcacgaaca
     7321 ccttgaataa tttgaaagac aacattgttt cgaaagcagg atctaaattt aaaaatatca
     7381 atgtcaattt taatgctaat aaagctgttg aatcaggtaa gggattttta gctaatattt
     7441 ggcaacaaat tgttaatttt ttccagcagt ttggtagtta ataaaaaagt ctagcttata
     7501 agctagactt tttttaccct aaataaatgt ttgtgttttt ttactaatct ttaaatgaat
     7561 atttaagaat aaatggcgca ataatagttg acagtataac aacaataaca agctcggaat
     7621 aagtagtgct tgaaataatt ttagcagcta atccaacttg aactataatg agagccatct
     7681 cgccacgact gaccattcca ccaccaattg ttaaagattc taattttgaa aaattaaagc
     7741 ctcgaccgac aaagtaccct ggaattaatt ttgataaaac agccaaagca gtaaagatta
     7801 atatcgtgtg aaggtgagac atcattccat cgaatttaag aggtagtgca attgaagcga
     7861 agaaaattgg gataaagaag gtgtaactaa gaagtgaaat tttatgttct accttatcaa
     7921 caaaagaagt ttgtccaata gctaatcctg caaaaaacga accaatgata gaactcattc
     7981 caactttatc ggctaaaatt gatagagaaa aacagataag gatagcaaga atagtatatt
     8041 tctcaaagaa atgaaccttt tcgattaatt tgaaaagagc aggtacaagg tatttcatac
     8101 aaataaataa gaaaacaaag aataacaatt gaatgataat ttgtaatccg atattagaag
     8161 agcttccatt tgttgcaata aagacactca gaagcaaaac agctaagaca tcatcagcaa
     8221 cagcagcacc taaaataata gcaccagtat ctgtttttac acgattgtat tcttgtaaaa
     8281 cttcaactgt gatagaaata ctagttgcag caaatacgag cccgtaaaag atagcagtat
     8341 tgatttggaa cccaaacaag cgtgtcaaga agtagaagag agccatagga acaataacac
     8401 cagtaattgc gaccagtaga ctgggcttaa gatacttctt taaaagatca aaattagctt
     8461 caagacccgc taaaaacatg agtaagataa caccaatttc tgacaggaca tgtaatacct
     8521 gattttgatg aacaagtcct agcattgcag gtccgatgat gatacctaca agtagttgtc
     8581 caacaacagc cggtattcca attctttttg aaataagagt tgcaagaacg cttgccaaca
     8641 aaatgattgt aatttgaatg ataatatgca ttttttcctc ctaatattaa taaaatgata
     8701 tcaatcatat tgtactgaat atttagaata ccatttttta agcctaaaa
//
LOCUS       DATRQA010000079         8699 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.079, whole genome shotgun sequence.
ACCESSION   DATRQA010000079 DATRQA010000000
VERSION     DATRQA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8699
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.079"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            39..662
                     /locus_tag="VBD27_001359"
     CDS             39..662
                     /locus_tag="VBD27_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEO7025368.1"
                     /translation="MIPLKKITVGTIVCLSFLGLTACSSSNTQQTSTSKSNVSQHKNI
                     KADHEELRLKFNKVKLGVKANNFKGGTSLAELKQLFGGEPNEKFDTPAGNVTLKGYRW
                     NVDDISITIQLLNDSSIVRSISNFKFIRDANITTKDYNSLKNGMSYNKVKELLGEPDD
                     ISQAVSSDKEELQAAWISGIQSSDSDPGINLTFENDKLTNKQQHGLK"
     gene            complement(931..1002)
                     /locus_tag="VBD27_001360"
     tRNA            complement(931..1002)
                     /locus_tag="VBD27_001360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(967..969),aa:Arg,seq:ccg)
     gene            complement(1075..2277)
                     /gene="rpsA"
                     /locus_tag="VBD27_001361"
     CDS             complement(1075..2277)
                     /gene="rpsA"
                     /locus_tag="VBD27_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO7025369.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIDGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVVEESAAAARKEVFSNIEVGSVVTGKVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVTVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSAGQEVTVKVL
                     EVNSDAERVSLSMKALEERPAQAEGEKEEKRQSRPRRPRRQEKRDYELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(2403..2474)
                     /locus_tag="VBD27_001362"
     tRNA            complement(2403..2474)
                     /locus_tag="VBD27_001362"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2440..2442),aa:Gln,seq:ttg)
     gene            complement(2480..2560)
                     /locus_tag="VBD27_001363"
     tRNA            complement(2480..2560)
                     /locus_tag="VBD27_001363"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2524..2526),aa:Tyr,seq:gta)
     gene            complement(2621..2851)
                     /locus_tag="VBD27_001364"
     CDS             complement(2621..2851)
                     /locus_tag="VBD27_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEO7025370.1"
                     /translation="MSKKDKKIEIQILDAQVTVNGTKVDGYQLVIGKKIVGQIAELDS
                     KFAVIKGEQVSGFYKNLDQAMGNIIEEYNLNR"
     gene            complement(2934..3956)
                     /locus_tag="VBD27_001365"
     CDS             complement(2934..3956)
                     /locus_tag="VBD27_001365"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEO7025371.1"
                     /translation="MTVNLDWDNLGFAYRKLPFRYISHFKDGKWDDGKLTDDATLHIS
                     ESSPALHYGQQAFEGLKAYRTKDGSIQLFRPDQNAERLQRTADRLLMPHVPTDKFIAA
                     VKSVVRANEEFVPPYGTGATLYIRPLLIGVGDIIGVKPAEEYIFTVFAMPVGSYFKGG
                     LTPTNFIVSKEYDRAAPNGTGAAKVGGNYAASLLPGKYAHEKQFSDVIYLDPATHTKI
                     EEVGAANFFGITKDNQFITPLSPSILPSITKYSLLYLAKERFGMEAIEGDVFVDELDK
                     FTEAGACGTAAVISPIGGIQNGDDFHVFYSETEVGPATRKLYDELVGIQFGDVEAPEG
                     WIYKVD"
     gene            complement(4070..6529)
                     /gene="parC"
                     /locus_tag="VBD27_001366"
     CDS             complement(4070..6529)
                     /gene="parC"
                     /locus_tag="VBD27_001366"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO7025372.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGFRKSAKSVGNVMGNFHPHGDSSIYDAMVRMSQDWKNRETLIEMH
                     GNNGSMDGDPAAAMRYTEARLSEIADYLLQDIDKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPKAKLDKLMEFLPGPDFPTGAI
                     IQGKDEIRKAYETGKGRVAVRSRTAIETLKGGKKQIIVTEIPYEVNKSVLVKRIDDVR
                     VNNKVPGIAEVRDESDRDGLRIAIELKKEADETIVLNYLFKYTDLQVNYNFNMVAIDD
                     YTPKQVGLSRILTSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVLSILDEVIALI
                     RASENKADAKENLKVSYEFSEAQAEAIVTLQLYRLTNTDIVTLREEEEELRQQITMLK
                     AIISDERTMYNVMKRELREVKKKFANTRRSELQELAETIEIDTASLIIEEDTYVSVTR
                     GGYVKRTSPRSFNASTVDELGKREDDELIFVSNAKTTQHLLMFTNLGNLAYRPVHELA
                     DIRWKDVGEHLSQNLVNFASNEEIIYAELVDDFTKETYFAVTSLGQIKRFERQEISPW
                     RTYKSKTAKYAKLKNVDDYVVTVAPIQLEDVILVTYNGYVLRFSINDVPVVGSKAAGV
                     KAMNLKDSDHIVSAFIANTTSLYLLTHRGSLKRMAIDVIPTTSRANRGLQVLRELKSK
                     PHRVFKAGPVYLEDSSFEFDLFSSVSNHEGDTFVLEIMSKTGKVYDVDLSQWSFSERT
                     SNGSFVSDKISDEEVFSVKIK"
     gene            complement(6663..8612)
                     /gene="parE"
                     /locus_tag="VBD27_001367"
     CDS             complement(6663..8612)
                     /gene="parE"
                     /locus_tag="VBD27_001367"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO7025373.1"
                     /translation="MTKQDITVTNYGDDAIQVLEGLDAVRKRPGMYIGSTDGTGLHHL
                     VWEIVDNAVDEALSGFGNRIDVIINKDGSITVTDHGRGMPTGMHAMGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEIIRDGAIYRQRFENGGKPVTTLK
                     KIGTAPKSKSGTSVSFMPDQSVFSTIDFKFNTIAERLKESAFLLKNVTLTLTDNRSEE
                     AEHLEFHYENGVQDFVEYLNEDKETLTPIMFFEGEEQEFHIEVALQYNDGFSDNILSF
                     VNNVRTKDGGTHETGLKSAITKSMNDYARKTGLLKEKDKNLEGSDYREGLSAILSILV
                     PEEHLQFEGQTKDKLGSPLARPIVDGIVSEKLTYFLMENGDLASNLIRKAIKARDARE
                     AARKARDESRNGKKSKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTAKAKMADIIKNEEINTMIHTIGAGVGPDFNLDDINYDKVIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEEGHVYIALPPLYKMSKGKGKKEIVEYAWTDIEL
                     EELRQKFGKGSLLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLM
                     GDKVPPRRQWIEDNVKFTLEENTVF"
BASE COUNT         2722 a         1796 c         1347 g         2834 t
ORIGIN      
        1 gttataatat ttaaaaatag aatttaggat aaataattat gataccgtta aaaaaaataa
       61 ctgttggaac gattgtctgt ctctcatttt tgggtttaac agcatgctct tcttcaaata
      121 ctcagcaaac ctctacttca aaatcaaatg tatcacaaca taaaaatatc aaagctgacc
      181 acgaagaatt acgtttgaaa tttaataaag taaagttagg ggttaaagca aataacttta
      241 aaggaggcac tagtctcgct gaattaaaac aattatttgg tggcgaacca aatgaaaaat
      301 ttgatacacc agcgggaaat gtcactctta aaggctatcg ttggaatgtc gatgatatct
      361 ccatcactat acaattatta aacgatagta gcattgttcg ttcaattagt aattttaagt
      421 ttattcgtga tgccaacatc acaacaaaag attataatag tttaaaaaat ggcatgtcat
      481 acaataaagt aaaagaactt ctaggagaac ctgatgatat ttctcaagca gtttcttcag
      541 ataaagagga actacaggct gcatggatta gtggcatcca gtcgtcagac agtgacccgg
      601 gaattaacct tacatttgaa aatgataaac tcactaacaa acaacagcat ggtttaaaat
      661 aaaaaggcat taacgccttt ttattaatac cttaattact tattagaaac attttattac
      721 acaatggttt aatcttagta ccttaactta gccccttaat ggtcatacta actaattatg
      781 atactaactt acacagattt caaactgatt ctcccttatt tgtcaaccct agttttactt
      841 atcagaaatg aggagaatca gtctgtgagg caaggttgga gataaactaa tataaagcct
      901 ataacataaa gctttatgga gcatctatta tccatcttaa gggaatcgaa cccctatccc
      961 aagaaccgga atcttgtgtg atatccatta cactaaagat ggtagtacat gttacattat
     1021 agcatacttt atcaacaaaa acaaaaagac ttgctttagc aagtctttta attattataa
     1081 ttcaatatca ccgaataagt cagccataga gaatccagtt tgtgtttctg gaagctcata
     1141 gtcgcgtttt tcttgacgac gtggacgacg tggacgtgat tgacgttttt cttctttttc
     1201 accttctgct tgcgctggac gctcttcaag agctttcatt gaaagtgata cacgttctgc
     1261 atctgaatta acttcaagaa ctttaacagt aacttcttgt cctgcagata atacgtcttt
     1321 aggattttca acacgtttgt gtgaaatttg tgaaatgtgt acaagtccat caatgccagg
     1381 taacacttca acaaaagcac caaagtcagt caagcgtttt actttacctt caataacatc
     1441 accagcagca agtttttgtt caacgccatc ccatgggcca ggtgtagttg ctttaagtga
     1501 aagtgataca cgaccagctt cttcatcaat tgaaagtact ttaacttcaa cttcttcacc
     1561 aacagttaca actgatttag gtgaaacatt gcgctcatgt gataattcag ttacgtgtac
     1621 aagtccatca acaccaccaa ggtcaatgaa agcaccaaag cttgttaaac gagcaacttt
     1681 accagttaca actgaaccta cttcaatgtt tgagaaaact tctttacgtg cagcagctgc
     1741 tgattcttca acaacttcac gacgtgaaag gatgaagcga ttttctgctg catcaacttc
     1801 tttgatttta gcatcaaatt cttgtccaac aaatttttca gtgttacgta caaaacgagt
     1861 atcaatcatt gaagctggga tgaaaccacg aagtccttca aattcaactg aaagtccgcc
     1921 tttaacagca cgtgtacctt taacagtaac aacttcacct tcacgaccaa caagtttgtc
     1981 ccatgcttta cgagcttcca agcgtttttt agatacaagg aaagttactg tatcagtatc
     2041 tttacctaca acttgacgaa gaacaagtac ttcaactgtg tcgccagctt taacaaaatc
     2101 attgatatca gcatcacgat cgttagtcaa ttcacgaagt gtcaagacac cttcaacacc
     2161 agtaccatcg ataacaacgt ttgcttgacc gttatcaact gttaaaactt ccgcagtgac
     2221 aacgtcacca ggattcactt cgctaacact gtttagcaaa tcttcaaatt cattcattct
     2281 aaaaaatcct ccaacaaaag ctagccgagc tagcttttga taacaatata ttttctcaag
     2341 gtaacccgca aggacaacaa atatctcttt gacggtgtca aacaaaagtt tgataccttt
     2401 gactggggta gctggattcg aaccaacgca tgagggagtc aaagtccctt gccttaccgc
     2461 ttggctatac cccaaataat ggaaagagag ggattcgaac ccccgaaccc gaaggagcgg
     2521 atttacagtc cgccacgttt agcctcttcg ctatctttcc gtaacaacac tgactattct
     2581 accacagatg ttgttagcac gcaagctttt taaataaata ttagcgatta agattatatt
     2641 cttcaataat atttcccata gcttgatcta aatttttata gaatccactc acttgctcac
     2701 ctttaatcac agcaaattta gaatctagct ctgcaatttg acctacaatt ttttttccaa
     2761 taacgagttg gtatccatca accttagttc cattaactgt tacttgagcg tctaaaattt
     2821 gaatttcaat tttcttatct tttttactca ctattaactc caatatctct tttattgttt
     2881 tttattgtac acaaaatact aaaagcttgc aagagcaagc ttttagtata gacttaatct
     2941 actttataaa tccacccttc tggtgcttca acatcaccaa actggatccc tactaactca
     3001 tcatatagtt tacgtgtcgc tggaccaact tctgtctcac tgtagaagac atggaagtca
     3061 tctccgtttt gaattcctcc aattggtgaa ataactgcgg ctgtaccaca tgcgccagct
     3121 tctgtgaatt tatctaattc gtcgacaaag acatctcctt caatcgcttc cataccaaac
     3181 ctttctttag caaggtaaag caatgaatat ttagtaattg aaggtaaaat tgaaggactc
     3241 aaaggggtga taaactgatt atctttagta attccaaaga aattagctgc tccaacctct
     3301 tcaattttag tatgtgtcgc tggatcaaga tagataacat ctgaaaattg tttctcatga
     3361 gcgtatttcc caggaagaag actagcagca tagttaccac caacttttgc agctcctgta
     3421 ccattcggtg ctgctctatc atattctttg gaaacaataa agttagttgg tgttaaacca
     3481 cctttaaaat aactacctac tggcattgca aataccgtaa aaatatattc ttcagcaggt
     3541 ttaactccaa taatatctcc tacaccaatt aataagggac gaatgtataa tgttgcccct
     3601 gtaccatacg gtggaacaaa ctcctcatta gcgcgtacaa cagatttaac cgctgcaata
     3661 aatttgtctg tcggaacatg tggcattaaa agacgatctg ctgttctttg aagtcgttct
     3721 gcattttgat ctggacgaaa taactgaata gagccatctt tagttctata agctttcaat
     3781 ccttcaaaag cttgttgccc atagtggagg gcaggagaac tttctgaaat atgtagcgtt
     3841 gcatcatctg tcaacttgcc atcatcccat ttcccatctt taaaatgcga aatgtatcgg
     3901 aatggtaatt ttctgtaagc aaaacctaaa ttatcccaat ctaaatttac tgtcataagg
     3961 tacctctttc tttgggtttc taactattgt acgctattat cattattttt tcaatattat
     4021 ttgtaaaatt cagataaatt accaaactag taaaaacaca aatatgtttt tacttaattt
     4081 taactgagaa tacttcttcg tcagaaatct tatcagatac aaaagagcca ttacttgtac
     4141 gttcagaaaa actccattga cttaaatcaa catcgtaaac ttttcctgtt ttagacataa
     4201 tctcaagtac gaaagtatct ccttcatgat tcgatactga actaaataaa tcaaattcaa
     4261 aagatgaatc ctctaaatat acaggtcctg ctttaaaaac gcgatgcggt ttggatttca
     4321 attcacgaag aacttgaagt cctcgattag cacgactagt tgtggggata acatcaatgg
     4381 ccattcgttt aagacttcct ctatgagtta ataaatataa acttgtggtg ttagctataa
     4441 atgcagatac gatgtgatca ctatctttta aattcatcgc ttttacacct gcggctttac
     4501 taccaacgac tggtacatca tttatactaa atctcaacac ataaccatta taagtgacaa
     4561 gtataacatc ctctaattgg ataggagcaa cagtcacaac gtaatcatct acatttttta
     4621 acttagcata tttagctgtt tttgacttat atgtccgcca tggactgatt tcttgacgtt
     4681 caaaacgctt tatttgccct aaactagtga cagcaaaata agtttcttta gtaaaatcat
     4741 ctactaattc agcataaata atttcttcat ttgaagcaaa gttaacaaga ttttgtgata
     4801 aatgttcacc aacgtctttc catcgaatat cagctaattc atgaactgga cgataagcaa
     4861 gattaccaag gttagtaaac atcaataaat gttgggtagt ctttgcattg ctcacaaata
     4921 tcaattcgtc atcttctcgc ttaccaagtt catcaacggt tgatgcattg aaagagcgag
     4981 ggctggtgcg ttttacatat ccgcctctag taacactgac gtatgtgtcc tcttcgataa
     5041 ttaaacttgc agtatcgatt tcaatagttt cagctaattc ttgaagttca gaacgtctag
     5101 tattggcaaa tttctttttg acctcacgta actcacgttt cattacgtta tacatcgtac
     5161 gttcatcact aataattgcc ttgagcattg tgatttgttg acgaagttct tcttcttctt
     5221 ctctcaacgt cacaatatca gtatttgtga gacgatataa ttgcaatgtg acgatagcct
     5281 ctgcttgagc ttctgaaaac tcatagctga ccttcaaatt ctctttcgca tcagctttat
     5341 tctcagaagc acgaattaat gcaatcactt catccaaaat agaaagaacc ctaatcagtc
     5401 cctcaacaat atggagacgt ttttcggctt tttctttatc aaattttgaa cgagcaataa
     5461 taatttctct tcggtgagcg atatagctag ttaatattct tgatagaccc acctgcttag
     5521 gggtatagtc atcaatagca accatattga aattataatt aacttgtagg tctgtatatt
     5581 taaaaagata atttaagaca attgtttcat cggcttcttt tttgagctca atagcgatac
     5641 gaaggccatc tctatcagac tcatcacgca cttctgctat tcctggaact ttattattaa
     5701 cacgaacatc atcgattcgt tttactaaaa ctgacttatt aacttcataa ggaatttcag
     5761 taacaataat ttgtttctta ccacctttta aggtttcaat agcagttcgc gagcgaaccg
     5821 ctactctccc cttaccagtc tcatatgcct tacgaatttc atcttttcct tgaatgatag
     5881 cgccagttgg aaaatctgga ccaggtagaa attccattaa tttatctaat ttagctttag
     5941 ggtgatcaat catgtacacg acagcatcaa tgacttctgc taaattatgt ggtggaatat
     6001 ctgtcgcata gcctgcagat attcctgttg ctccgttgac taaaagatta ggaaaggcag
     6061 ctggtaaaac agttggttct ttctctgtat catcaaaatt ccacgcaaag ggtacagtat
     6121 tcttatcaat atcttgaaga agataatcag caatttctga tagacgggct tccgtataac
     6181 gcatagctgc cgcaggatct ccatccatag aaccattatt tccgtgcatt tcaatcaatg
     6241 tctcacgatt tttccaatct tgagacatac gaaccatcgc gtcatagata gaggaatccc
     6301 catgaggatg gaaattcccc ataacattac cgactgattt tgcagatttg cgaaatccct
     6361 tttcaaaagt attaccatct ttattcattg aataaaggat tcgacgttga acaggtttta
     6421 agccatcacg aatatccggc aacgcccgtt cttgaatgat atacttagaa tagcgcccaa
     6481 aacgctctcc cataatgtcc tcaagggaca tattttgaat attagacata tttttaattt
     6541 tctcctagtt ggagtggctt agtattattt ttccacttat ttgaaatgaa tgattggctc
     6601 tcttgtctaa cgcatacaat attacttgta cactataaaa catgaataac caaggctctt
     6661 tattaaaata ctgtattctc ttctaaagta aacttgacat tatcttcaat ccattggcgt
     6721 cttggaggaa ctttatcacc catcaagaca ttaactcgac gttctgctct tgctaaatct
     6781 tcaattgtta cacgaattaa tgtccttgtc tcagggttca tagtggtttc ccacaattga
     6841 tcggcattca tttctccaag ccctttatat cgttgtaata gtgagccttt accaaatttt
     6901 tgtcgtaatt cttctaactc tatatccgtc caagcatact caacaatttc tttcttacct
     6961 tttccttttg acattttata caaaggtggc aaagcaatat agacatgtcc ctcttcaaca
     7021 agaggtctca tatagcgata aaagaaagtt aataatagtg tctgaatatg agctccgtca
     7081 gtatcagcat cggtcataat gataacttta tcataattaa tatcgtcaag attaaaatca
     7141 ggaccaacac ctgcaccaat ggtatggatc attgtattaa tttcttcgtt ttttataata
     7201 tcagccatct ttgccttagc tgtattcaat acttttcctc gcaacggtaa aatagcttga
     7261 aactttctat cacgaccttg cttagcagag ccacctgcag agtctccttc gactaaatag
     7321 agttcatttt tcttagcatt ttttgattgt gcaggggtta attttccaga aagtagacct
     7381 ttatcttttt tcgacttttt cccattccta ctttcatcac gtgctttacg tgcagcttca
     7441 cgcgcatcac gtgcttttat tgccttacgg attaaattag atgctaaatc tccattttcc
     7501 ataagaaagt aagttaactt ttcagaaaca ataccatcaa caattggtcg tgctaaggga
     7561 cttcctagtt tatctttagt ttgaccttca aattgcaagt gctcctcagg tacaagaata
     7621 gataaaatgg ctgatagccc ctcacgataa tcagaacctt caaggttttt atctttttct
     7681 ttaagaagtc ctgttttacg tgcatagtca ttcatagact ttgtaatagc tgatttcaag
     7741 ccagtttcat gtgttccacc atctttagta cgaacattat tgacaaaaga aaggatatta
     7801 tctgaaaaac catcattata ctgcaaagca acttcgatat gaaattcttg ttcttcacct
     7861 tcgaaaaaca tgattggtgt caacgtttct ttatcttcat ttaaatactc tacaaaatct
     7921 tgaacaccgt tttcataatg aaattctaga tgctctgctt cttctgaacg attatctgtt
     7981 aaggttaaag taacattttt taagagaaag gcagattcct ttaagcgctc tgcaattgta
     8041 ttaaacttaa aatctatcgt tgaaaatact gattgatctg gcataaagct gacagaagtt
     8101 ccagattttg attttggtgc cgtgccaatt tttttcagag ttgtaacggg cttaccacca
     8161 ttttcaaaac gttgtctgta aatagctccg tcgcgaatga tttcaacttc taaccaagaa
     8221 gacaaagcat taactacaga tgatccaaca ccatggagac cacctgatgt tttataacca
     8281 ccttgtccaa attttccacc tgcatgaagc accgtgaaga tgacttcaac agtaggtttt
     8341 cccatagcat gcattccagt tggcatacca cgtccatggt ctgtaacagt tatactccca
     8401 tctttgttaa taatcacatc aatgcgattt ccaaaacctg atagagcttc atcaactgca
     8461 ttatctacaa tttcccacac taaatgatgt aaaccagttc catctgtgga accgatatac
     8521 atccctggac gcttacgaac agcatctaat ccttctaaga cttgaatagc atcgtcgcca
     8581 taattcgtca ctgttatatc ttgtttagtc aaagtgacct ccaaattttt tcatcttttt
     8641 tatattacaa gttttttgct gtttttgcaa aattattttt ttaaaaatag caaaaagac
//
LOCUS       DATRQA010000080         8686 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.080, whole genome shotgun sequence.
ACCESSION   DATRQA010000080 DATRQA010000000
VERSION     DATRQA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8686
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.080"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(81..1508)
                     /locus_tag="VBD27_001368"
     CDS             complement(81..1508)
                     /locus_tag="VBD27_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO7025374.1"
                     /translation="MTKEYQNYVNGEWKSSVNQIEILSPIDDSSLGFVPAMTREEVDH
                     AMKAGREALPAWAALTVYERAQYLHKAADIIERDKEEIATVLAKEISKAYNASVTEVV
                     RTADLIRYAAEEGIRLSTSADEGGKMDASTGHKLAVIRRQPVGIVLAIAPYNYPVNLS
                     GSKIAPALIGGNVVMFKPPTQGSVSGLVLAKAFAEAGLPAGVFNTITGRGSEIGDYIV
                     EHEEVNFINFTGSTPVGQRIGKLAGMRPIMLELGGKDAGIVLADADLDNAAKQIVAGA
                     YDYSGQRCTAIKRVLVVEEVADELAEKISENVAKLSVGDPFDNATVTPVIDDNSADFI
                     ESLVVDARQKGAKELNEFKRDGRLLTPGLFDHVTLDMKLAWEEPFGPILPIIRVKDAE
                     EAVAIANKSDFGLQSSVFTRDFQKAFDIANKLEVGTVHINNKTGRGPDNFPFLGLKGS
                     GAGVQGIRYSIEAMTNVKSIVFDMK"
     gene            complement(1658..3391)
                     /gene="ptsP"
                     /locus_tag="VBD27_001369"
     CDS             complement(1658..3391)
                     /gene="ptsP"
                     /locus_tag="VBD27_001369"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO7025375.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVLSDPEMINQIKETIRAKQVNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITERVQDGQ
                     LIAVNGITGEVIIEPTEAQISAFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNENGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFEEEKANLLADGVAVAEGIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALSECSTMEEV
                     IELQKEYVDFD"
     gene            complement(3396..3659)
                     /locus_tag="VBD27_001370"
     CDS             complement(3396..3659)
                     /locus_tag="VBD27_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEO7025376.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAIEETMTKEGLA"
     gene            4042..4266
                     /locus_tag="VBD27_001371"
     CDS             4042..4266
                     /locus_tag="VBD27_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEO7025377.1"
                     /translation="MAAITVFSKNNCMQCKMTKKFLDQHGADFEEINIDEKPEKIEYV
                     KNLGFSAAPVIEAGNVVFSGFQPSKLKELV"
     gene            4344..6503
                     /gene="nrdE"
                     /locus_tag="VBD27_001372"
     CDS             4344..6503
                     /gene="nrdE"
                     /locus_tag="VBD27_001372"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO7025378.1"
                     /translation="MSLKNIGDVSYFRLNNEINRPVNGQIPLHKDKEALTAFFKENVQ
                     PNSKAFDSITDKIAYLLKYDYLEEAFLNKYRPEFIEELSTKLFDKKFRFKSFMAAYKF
                     YQQYALKTNDGEYYLESIEDRVLFNALYFADGDEELATDLALEMISQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNAIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGF
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARNNQEMYLFSPYSIEREYGVPFSYIDITEKYDELVANPNI
                     TKTKINARDLETEISKLQQESGYPYIINIDTANRTNPVDGKIIMSNLCSEILQVQKPS
                     LINDAQEYLEMGTDISCNLGSTNVLNMMTSPDFGKSIKTMTRALTFVTDSSNIEAVPT
                     IKNGNAQAHTFGLGAMGLHSYLAKNHIEYGSPESIEFTDIYFMLMNYWTLVESNNIAR
                     ERQTTFVGFEKSKYADGTYFDKYVSGKFVPQSDKVKSLFTNHFIPEAKDWENLRYAVM
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLATDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSMTLFLRSELPKELYEWKTESKQTTRDLS
                     ILRNYAFNKGVKSIYYIRTFTDDSSEVGANQCESCVI"
     gene            6706..7665
                     /gene="nrdF"
                     /locus_tag="VBD27_001373"
     CDS             6706..7665
                     /gene="nrdF"
                     /locus_tag="VBD27_001373"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO7025379.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSAQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEEIFEWTNNNEFLQEKARIINDIYANGNALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMHDDDYNYGL"
     regulatory      7750..7848
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7905..8468
                     /gene="thiT"
                     /locus_tag="VBD27_001374"
     CDS             7905..8468
                     /gene="thiT"
                     /locus_tag="VBD27_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEO7025380.1"
                     /translation="MSKNNNTTCLIETAIFAALAMALSMIPDFASWFTPSFGAIPLIL
                     FALRRGTKYGLFAGLIWGLLHFVLSKVYYLSLSQVFIEYILAFISMGLAGVFSAKFKD
                     ALSSSSKTKALSLALSGAILATFVRYVWHYIAGVIFWASYAPKGMSATLYSLSVNGTA
                     GLLTLFFVVISIIILVISYPSFFLPKK"
     gene            complement(8553..>8686)
                     /locus_tag="VBD27_001375"
     CDS             complement(8553..>8686)
                     /locus_tag="VBD27_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3169 domain-containing protein"
                     /protein_id="HEO7025381.1"
                     /translation="IYFVLFFISFVFQKVEIVAIIITTSIHIYILIKSLKAARHFYR"
BASE COUNT         2747 a         1655 c         1452 g         2832 t
ORIGIN      
        1 aagattatat aattctttat ttttaatgta aaagaggttt ataaaacgac tcaactgaca
       61 atcgttgagt cgttttacat ctatttcata tcaaaaacaa tcgattttac atttgtcatt
      121 gcttcaattg aatatctgat accttgaaca cctgcaccag atcctttgag tcctaagaat
      181 gggaagttat caggaccacg tccagtctta ttgttaatgt gaactgtacc aacttcaagt
      241 ttatttgcta tatcaaatgc tttttggaaa tcacgtgtaa agactgatga ttgtaatcca
      301 aaatcagatt tgttggcaat agcaacagct tcttctgcat ccttgacacg aataattggg
      361 agaattggtc caaaaggctc ttcccaagct agtttcatat ctaaagtaac atgatcaaac
      421 aatcctggag ttaatagacg accatcacgt ttaaattcat tcaattcttt cgcacctttt
      481 tgacgtgcat ctactactaa gctttcaata aagtcagctg aattatcatc aataaccggt
      541 gtcaccgttg cattatcaaa tggatcacct actgataatt ttgctacatt ttcagatatt
      601 ttttctgcca attcatctgc aacttcttca acgacaagca cacgcttaat tgccgtacag
      661 cgttgtccag agtaatcata agcacctgca acgatttgtt tagcagcgtt atcaaggtca
      721 gcatcagcta agacgatacc tgcatcctta ccgcccaact caagcataat tggacgcatt
      781 cctgccaact taccaatacg ttgtccaact ggcgttgatc ctgtaaagtt aataaaatta
      841 acttcttcat gctcaacaat gtaatctcca atctcagaac cgcgtcctgt aatagtatta
      901 aagacacctg ctggaagacc tgcttctgca aaagcttttg ctaaaacaag tcctgagact
      961 gaaccttgtg ttggtggttt aaacatcaca acgtttccac caattagagc tggcgcaatt
     1021 tttgatcctg agaggttaac agggtaatta taaggtgcga ttgctaaaac gatacctact
     1081 ggttgacgac gaataacagc caacttatga cctgttgaag catccatttt tccaccttcg
     1141 tcagctgaag ttgataaacg aattccttct tctgctgcat aacgaataag atcagctgtc
     1201 cttacaacct cagttactga agcattgtaa gctttagaaa tttcttttgc taaaacagta
     1261 gcaatttctt ctttatcacg ttcaataatg tctgcggctt tatgaaggta ttgtgcacgt
     1321 tcatatactg ttaaagcagc ccaagctggt aaagcctcac gacccgcttt catagcatga
     1381 tcaacttctt ctcgagtcat cgctggcacg aatcccaatg aagaatcatc aattggtgac
     1441 aaaatctcaa tctgattaac agatgatttc cattcgccat tgacataatt ttgatattct
     1501 tttgtcaaag taaatctcct tttacattgt aataggttta ttctatcact ttatctattt
     1561 atttcaatta tctgacatat ttttcttaga aacttaacaa tatccaaact ttcaataaaa
     1621 aagtagagat tttttaatcc ctactttttt attattctca gtcaaagtca acgtattctt
     1681 tttgaagttc gataacttct tccatagttg aacactctga aagagcacga ttagcgtatt
     1741 cttccatttt agctgtgtcc aattttttca ttaagctacg tgtacgaagt actgaggttg
     1801 ctgacattga gaattcatcc aatcccatac caacaagaag tggaaccgca gtttgatcac
     1861 cggccatctc accacacata cctgcccatt taccttcagc atgagcagct ttgataacgt
     1921 tgttaatcaa acgaaggatt gatggattgt atggttgata gaggtatgaa acttgctcat
     1981 tcatacggtc cgcagccatt gtgtattgga taagatcatt agtaccaatt gagaagaaat
     2041 caacttcttt tgcaaattgg tctgcaagca tagctgccgc tgggatttca atcataatac
     2101 caacttcaat gccttcagct actgctacac cgtcagctaa caagttcgct ttctcttctt
     2161 caaatatagc tttagcagca cggaattctt taagcaaagc aaccattggg aacatgatgc
     2221 gtagctgtcc atgaactgaa gcacgaagaa gtgctcggat ttgtgtgcgg aacattgcat
     2281 caccagtttc agaaattgaa atacgcaatg cacggaatcc taagaatggg ttcatctctt
     2341 ttggaagatc aaagtaagga agctctttat ctccaccgat atccattgta cgaacaacaa
     2401 ctggtttacc attcataccc tcaagaactg ctttatatgc ttcatattgc tcatcttctg
     2461 ttgggaaatc ttgtgaatcc atatataaga actctgtacg gtaaagacca actgcttctg
     2521 ctccattttc gttaacaccc tcaacatctt taggtgtacc gatattagca gctaattcaa
     2581 aatgtttacc atcagcagta actgtttgtg catccttaag aagtgcccat tcagcttttt
     2641 gcttggcata agcttcacct gctgccttga aggctgaaat ttgagcttca gttggttcaa
     2701 tgataacttc accagtaata ccattaacag caatcaactg accatcttga acacgttcag
     2761 taatatcatt tgtaccaaga acagctgcga tttcgagtgt acgtgccata atagctgagt
     2821 gacttgtacg ccctccaata tttgttacaa aagctttaac aaattgttta tttaattgag
     2881 ctgtatcaga aggtgtcaaa tcgtgtgcaa taacgattga ttcttcattg atagttgccg
     2941 ggtttggtaa tttaacaccc aaaagatgtg ctaagacacg tttggcaaca tcacggatat
     3001 ctgctgcacg ttcttgcatg tatgggttat cttccatacc ttcaaagata gtgatgaaca
     3061 tatcagtcac ttctttaaga cctgtttctg catttacttg ttttgcacga atagtctctt
     3121 taatttgatt aatcatttct gggtcagaaa gaaccattaa atgagcatca aatacagctg
     3181 cggcttcttc gcctaagctt tctactgcct tctcacggat aacagaaagc tcgtcttgtg
     3241 aagcttggag tgcaacatca aggcgagctt cttctgcgtt tgtatcttcg actgttacag
     3301 tctcaaatga caaatccggt tggacaagta gatatgcttt agcaactgca acaccatcag
     3361 aggctgcgat tcctttaagc atttctgtca ttttcttatg ccaatccttc ttttgtcatt
     3421 gtttcttcga tagctgcaat tgcatcatct gcatctgcac cttctgctga aatagtaaca
     3481 tcagcacctt gaccaacacc aagactcata acacccatga ttgatttaag gtttactgct
     3541 ttacctttgt aatcaagcgt gatgtctgaa gcaaatttgc tagctgtttg gacaagcaaa
     3601 gtagctggac gtgcgtgaat acctgtttct gcaacaatgt ggaaatcttt tgaagccata
     3661 ttatggttct ccttttgtca tttgtaaata tttgagtcac ctattggtaa cccttacaag
     3721 ttaaaagtat aacatattgc tttttatttt tcaagattaa atgtctcaaa cagaaaaaaa
     3781 cttgaatgtt ttccacagct tttcatatta aaaatgagag tcttagaatt taaaaataga
     3841 attctaaaaa tgattttttt atgttcttct gaaaggaatt ggaaaacagg ctgaaagctt
     3901 ttaaactaac cctaaaaacg aaatatagtg tctcatttta ttttaagaca ctatatattg
     3961 tgttttgtta gaaataaaac acttgctttt tccattcttt ttttatatgc tattaccatg
     4021 aaaaaaaatt aggaggacaa catggcagct attactgttt tttcgaaaaa caactgtatg
     4081 caatgtaaaa tgactaagaa atttcttgac cagcacggcg ctgattttga agaaattaat
     4141 attgatgaaa aaccagaaaa aattgaatat gtgaaaaatc tcggcttttc tgcggctcca
     4201 gtgattgaag ctggtaatgt tgttttttca ggtttccaac caagcaaact aaaagaactt
     4261 gtttaagcaa attagagtct gggaataaag caattaattg ctttattccc attttgtatt
     4321 gttaagttaa gaggataaat tcaatgagtt taaaaaatat tggcgatgta tcttactttc
     4381 gcctaaataa tgaaattaac cgtcctgtta atggacaaat cccactccat aaagataaag
     4441 aagccttaac tgcttttttc aaagaaaacg tacagcctaa ttcaaaagct tttgactcaa
     4501 tcactgataa aattgcttac ctccttaagt atgattatct tgaagaagct ttcctcaata
     4561 aatatcgtcc tgaatttatt gaagaattat ctaccaaact cttcgataaa aagtttcgtt
     4621 ttaaatcctt catggcagcc tataagtttt atcagcagta tgcattaaaa acaaatgatg
     4681 gagaatacta tttagaaagt attgaagatc gcgttctctt taacgctctt tattttgcag
     4741 acggtgatga agaactagca actgacttag ctctagaaat gatttcacaa cgctatcaac
     4801 ctgcaacacc aagtttttta aatgctggtc gaagtcgtcg cggagaatta gtctcatgtt
     4861 tcttaatcca agtaactgat gatatgaatg ctattggtcg ttcgattaat tcagctcttc
     4921 aattatctcg tattggtggt ggggttggaa tatcacttag taaccttcgt gaggctggag
     4981 ctcctatcaa aggttttgct ggtgctgctt ctggagtcgt tccagtcatg aaattatttg
     5041 aagacagttt ttcttactcc aaccaacttg gacaacgcca aggtgctgga gttgtttacc
     5101 ttgacgtttt ccatccagat attatttcat tcctatcaac taaaaaagaa aatgctgatg
     5161 aaaaagtacg tgttaaaaca ttgtcattag ggattactgt acccgataaa ttctatgaac
     5221 tagcacgtaa caatcaagaa atgtacttgt ttagtcctta tagtatcgaa cgtgaatatg
     5281 gtgttccttt cagctatatt gatattaccg aaaaatatga tgaattagtc gctaacccta
     5341 atatcactaa aacaaaaatc aatgctcgtg atttagaaac agagatttct aaacttcaac
     5401 aagaatctgg ttatccttac atcatcaata ttgacactgc taatcgtact aatcctgttg
     5461 acggaaaaat tattatgtca aatctttgct ctgagatttt gcaagtacaa aaaccaagtc
     5521 ttatcaatga tgctcaagaa taccttgaaa tgggaactga tatttcttgt aaccttggct
     5581 caactaatgt tttaaatatg atgacatctc ctgattttgg aaaatcaatt aaaacaatga
     5641 ctcgtgctct aactttcgtt actgactcat caaatattga agctgttcca actattaaga
     5701 acggaaatgc tcaagcacat acatttggac ttggagcaat gggactccac tcctatttgg
     5761 caaaaaatca tatcgaatat ggtagtccag aatcaattga atttactgat atttatttca
     5821 tgctaatgaa ctactggacc ttagtagaat caaacaatat tgcccgcgaa cgtcaaacaa
     5881 catttgtagg ttttgaaaaa tcaaaatacg ctgacggtac atactttgat aaatatgtat
     5941 ctggaaaatt tgttccacaa tctgacaaag taaaatccct atttactaat cattttatcc
     6001 ccgaagctaa agattgggaa aaccttagat acgcagttat gaaagacgga ctatatcatc
     6061 aaaatcggct tgcagttgct ccgaatggtt ctatttcgta tatcaacgat tgttcagcat
     6121 ctatccaccc tattacacaa cgtattgaag agcgtcaaga gaaaaaaatc gggaaaattt
     6181 attatccagc taatggctta gctacagata ctatccctta ttacacttct gcctatgata
     6241 tggatatgcg taaagtaata gacgtctatg ccgcagcaac tgagcacgta gaccaaggtc
     6301 tctcaatgac actcttttta cgaagcgaat taccaaagga gttgtacgag tggaaaacag
     6361 aaagtaagca gactactcgc gacctttcaa tcctacgtaa ctatgctttc aacaaaggtg
     6421 tgaaatcaat ctactatatc cgtactttta cagatgatag tagcgaagtc ggagctaatc
     6481 aatgcgaaag ttgtgtcatc taacgaagaa actctactta aaacatcaga aactagactg
     6541 agataaacta tctcagtttc atgatgttat cttattttct aaggtatttt catctttatt
     6601 tttctaaaaa tcgataatac ttgaaatatt gctagttcag aattctatat tctactcttc
     6661 ttccatcatc attatataaa aacctattta ccattggaga aaaccatgac aacttactac
     6721 gaagctataa actggaacga aattgaagat gttattgata aatcaacttg ggaaaaacta
     6781 accgaacaat tttggctcga tacacgtatc cctttatcaa atgacttaga cgattggcgc
     6841 aaactttccg ctcaagaaaa agatcttgtt ggcaaggttt ttggaggctt aaccctactt
     6901 gataccatgc aatcagaaac tggtgttgaa gctattcgtg ccgatgttcg cacgcctcac
     6961 gaagaagctg tcttaaacaa tattcaattc atggaatctg ttcacgctaa atcttattct
     7021 tcaattttct caactttaaa tactaaatca gaaattgaag aaattttcga gtggactaat
     7081 aataatgagt tccttcaaga aaaagcacgt attatcaatg acatttatgc taatggaaat
     7141 gcccttcaaa aaaaggtggc ttccacctac ctcgaaactt tcctttttta ttctggcttt
     7201 ttcacacctc tttactattt gggaaataat aagttagcaa atgttgctga aatcattaaa
     7261 ttaattattc gtgatgaatc tgtacatggt acttatatcg gttacaaatt ccagcttggt
     7321 tttaacgaat taccagaaga tgagcaagag aattttcgtg attggatgta tgacctcctt
     7381 tatcagctgt atgaaaacga agaaaaatac accaagacac tttatgatgg cgtaggatgg
     7441 actgaagaag ttatgacctt tttacgctac aatgctaata aagctcttat gaatttagga
     7501 caagatcctt tattcccaga tacagcaaat gatgtcaacc caattgttat gaatggtatt
     7561 tcaacaggaa catcaaacca tgacttcttc tctcaagtag gtaatggtta cctacttggt
     7621 agcgttgaag ctatgcatga tgatgactat aactatggat tataagaaaa agacactgat
     7681 ttagtgtctt tttctagtta aatacaaatt tcttgacaag caaacccttt caagatagaa
     7741 tgttagagta aatacacaag ggagtgcctt gagctgagat tgcagatatg caaaatcctc
     7801 taacctgatc tcgttaggac gagcgtagga attgtggttt gaattggtta aacatgacaa
     7861 atgacaaaag aatctccttt gctgtataaa aaaggagatt ttttatgtca aaaaataaca
     7921 acactacctg tcttattgaa actgctattt ttgcagcgct tgctatggct ttatcaatga
     7981 ttcctgattt tgcgtcatgg ttcactccct cttttggagc aattcctctg atactttttg
     8041 cattacgtcg tggtacaaaa tacggtctat ttgctggctt aatttggggc ttattgcact
     8101 ttgttctttc caaagtatat taccttagtt tatcccaagt ttttatagaa tacatcttag
     8161 catttataag tatgggactt gcaggcgtat tttctgctaa atttaaagat gcacttagca
     8221 gcagctctaa aactaaagct ttatcattag cattatctgg agcaatatta gcaacatttg
     8281 ttcgttatgt ttggcattat atcgctggtg ttattttttg ggctagttat gcaccaaaag
     8341 gtatgtctgc tacattatac tcattaagcg tcaatggtac tgctggtttg ttaactctat
     8401 tttttgtagt cattagtatt attattttag ttatcagcta ccctagcttc tttttaccta
     8461 agaaatagaa aaaataaaag gactgatttt cagagaatca atccttttat tttacactaa
     8521 tataagtttt ttgataaaaa atctagctac tactatctat aaaaatgacg tgctgctttt
     8581 aaagacttaa tgagaatgta gatatgaata gatgttgtta taataattgc tacaatctcc
     8641 actttttgaa aaacaaatga gataaagaat aatacaaaat agatcg
//
LOCUS       DATRQA010000081         8657 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.081, whole genome shotgun sequence.
ACCESSION   DATRQA010000081 DATRQA010000000
VERSION     DATRQA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.081"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            65..1699
                     /locus_tag="VBD27_001376"
     CDS             65..1699
                     /locus_tag="VBD27_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO7025382.1"
                     /translation="MSQKTTKVSQQEQMVKGTAWLTAGNFISRLLGAIYIIPWYVWMG
                     KHAAEANALFGMGYEIYALFLLISTVGIPVAVAKQVSKYNTLGKEEMSIYLVRKILQF
                     MLILGGVFALIMYIGSPLFASLSKGGQELVPILRSLTLAVLVFPSMSVLRGFFQGFNN
                     LKPYAISQVAEQIIRVIWMLLTAFYIMRLGSGDYIAAVTQSTFAAFVGMFASIAVLLY
                     FLWRYNMLSALISKTPEHIKLDTKEILIETVKEAIPFIITGAAIQIFKLIDQFSFGNT
                     MALFTNYSSEELRVMFAYFSSNPGKVTMILIAVATAIAGVGIPLLTENFVKNDKKAAA
                     RLVVNNLQMLLMFLLPAVAGSVILAKPLYTVFYGLPQGQALGLFVISLIQTIILSIYT
                     VLAPMLQALFENRKAIIYFLYGLVAKVILQVPSIFLFHAYGPLFSTTVALCIPVILMY
                     LKIHEITGFKRQAIRRTSALVLILTLLMSFIISMIIWLMNLVIVPDSRLVSLVYIIVI
                     GAIGLGVYGFMALATHLLDKMIGSRAQDLRRKLHLN"
     gene            1834..3054
                     /gene="pepT"
                     /locus_tag="VBD27_001377"
     CDS             1834..3054
                     /gene="pepT"
                     /locus_tag="VBD27_001377"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEO7025383.1"
                     /translation="MSYEKLLERFLTYVKINTRSNPNSTQTPTTQSQVDFALTVLKPE
                     MEAIGLKDVHYLPSNGYLVGTLPATSDRLRHKIGFISHMDTADFNAENITPQIVDYKG
                     GDIELGDSGYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASH
                     PEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLEL
                     TFEGRNVHPGTAKNQMINALQLAIDFHSQLPENERPEQTDGYQGFYHLYDLSGTVDQA
                     KSSYIIRDFEEVNFLKRKQLAQAIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTP
                     INIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGGENMHGRFEFVSLQTM
                     EKAVDVILGIVAKD"
     gene            3083..3574
                     /locus_tag="VBD27_001378"
     CDS             3083..3574
                     /locus_tag="VBD27_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEO7025384.1"
                     /translation="MIKLFGKIRYHWQPELSWAIIYWSIAIAPIFIGLSLLYERTEIP
                     SQVFVLFAIFIVLVGIGFHRYFVIEEDGYLRIVSFNFLRRSKFPIEDIAKIEVTKSSV
                     TIKFNNNHERIFYMRKWPKKYFLDALAIEPKFKGEVELLDNLIKMDYFECYRYDKKAL
                     TKS"
     gene            complement(3561..3758)
                     /locus_tag="VBD27_001379"
     CDS             complement(3561..3758)
                     /locus_tag="VBD27_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEO7025385.1"
                     /translation="MKVALIPEKCIACGLCQTYSNIFDYQDDGIVKFSDTDNLEKEIP
                     SSDQDTVLAVKSCPTKALTIL"
     gene            3804..4328
                     /locus_tag="VBD27_001380"
     CDS             3804..4328
                     /locus_tag="VBD27_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO7025386.1"
                     /translation="MAKKPWEKKVVENNSHRKDKITRTSRGVVSSTPWITAFLSAFFV
                     IVVAILFIVFYTSNRGEDRAKETSGFYGASSQKVNSSKTKKASTSKKTTSSSKTKTSD
                     KSTTSSSTVASSSETSTSSSVNGGTIVVQPGEGAASIAARAGISVARLQELNPGHMTS
                     GYWYANPGDPVVVN"
     gene            4339..5022
                     /locus_tag="VBD27_001381"
     CDS             4339..5022
                     /locus_tag="VBD27_001381"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO7025387.1"
                     /translation="MNSINIAIDGPASSGKSTVAKIIAKNLNYTYLDTGAMYRCATYL
                     ALQHGYEAQDVSKILGLLAERPISFGKAEDGSQTVFLGTEEVTLAIRQNDVTNNVSWV
                     SAIPEIREELVNQQRRIAKDGAIIMDGRDIGTVVLPDAELKIFLVASVDERAERRFKE
                     NQEKGIESDFETLKSEIAARDYKDSHREVSPLKAAEDAIEFDTTGVSIEGVVTFIQEK
                     AEKIIDMKN"
     misc_feature    5043..5168
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            5183..5713
                     /gene="infC"
                     /locus_tag="VBD27_001382"
     CDS             5183..5713
                     /gene="infC"
                     /locus_tag="VBD27_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO7025388.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQAIADD
                     ANVDLVLIQPQATPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPIPDKK"
     gene            5753..5953
                     /gene="rpmI"
                     /locus_tag="VBD27_001383"
     CDS             5753..5953
                     /gene="rpmI"
                     /locus_tag="VBD27_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO7025389.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFHAFTSHRFHGKTKKQRRHL
                     RKATMVSSGDFKRIKAMLTRLK"
     gene            6011..6370
                     /gene="rplT"
                     /locus_tag="VBD27_001384"
     CDS             6011..6370
                     /gene="rplT"
                     /locus_tag="VBD27_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO7025390.1"
                     /translation="MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLGK"
     gene            complement(6613..>8657)
                     /locus_tag="VBD27_001385"
     CDS             complement(6613..>8657)
                     /locus_tag="VBD27_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO7025391.1"
                     /translation="HTDFNLGLENSYQLFLTIINPIPLGLLIIGLALYVKRTKAFYIT
                     AFITYAIVNILLIANAIYYREFSDFITVSAVLASSKTSAGLGDSALNLLRIWDLVYVF
                     DFIILIFLFATKKIHLDNRPFNKRASFSITALSGLLFSINLFLAEIDRPELLSRGFSN
                     TYIVKALGLPSFSIYSGNQTYQAQKERNGATAQELATAKKYVAEHYAKPNPEYYGIGK
                     GRNVIMIHLESFQQFLIDYKLNVDGKEHVVTPFINSLYHSKETISFSNFFHQVKAGKT
                     SDAETLMENSLFGLSSGSFMVNYGGENTQFAAPHILAQNGGYSSAVFHGNVGTFWNRN
                     NAYKQWGYDYFFDSSYFSKQTKDNSFQYGLNDKYMFADSIKYLEHMQQPFYTKFITVS
                     NHYPYTSLKGESDEEGFPLAKTNDETINGYFATANYLDTALKSFFEYLKAAGVYDNSI
                     IVMYGDHYGISNTRNPSLAELLGKDPETWSEYDNAMLQRVPYMIHIPGYSKGFISNTY
                     GGEVDNLPTLLHILGIDTSKYTQLGQDLLSKDNKQMVAMRTTGQYITPKYTNYSGHLY
                     YTDSGQEITNPDETTKAEIKTIRDATNKQLSTSDSIQTGDLLRFDENNGLKTVEVEKF
                     NYTHSLKALKAKERKLKDRSTSIYSKHNNKSTVDLFHAPSYLELQDPNKTHKTSKK"
BASE COUNT         2794 a         1356 c         1683 g         2824 t
ORIGIN      
        1 aagtgatatt tctgagattt ttgtctttta aatgctaaat aatattgaaa atagaaagtt
       61 taccatgtca caaaaaacaa caaaagttag tcagcaagaa caaatggtca aagggacggc
      121 ttggttaaca gcagggaatt tcattagtcg cttgctggga gccatctata ttatcccttg
      181 gtatgtttgg atgggaaagc atgctgccga agcaaatgct ttatttggga tgggatatga
      241 aatctatgct ttatttcttt tgatatcaac tgttggaatt ccagtagcag ttgcaaaaca
      301 agtatctaag tataacactt taggtaaaga agagatgagc atttatctgg ttcggaaaat
      361 cttgcagttt atgctgattt taggtggagt ttttgcatta attatgtata ttggttcacc
      421 tctatttgct tcacttagta aaggagggca agaactcgtt ccaatattgc gtagtctaac
      481 tctagctgtt ttagtttttc cttcaatgag tgttttacgt ggtttttttc aaggttttaa
      541 taatttaaaa ccgtatgcca ttagtcaagt cgctgagcag attatacgtg tcatatggat
      601 gttattaact gctttctata ttatgcgtct aggatcagga gattatattg ctgctgtgac
      661 acagtcaaca ttcgccgcct ttgtcggtat gtttgctagt atagctgtgc tgctttattt
      721 cttatggcgt tataatatgc tatctgcctt gattagtaaa actccagagc acattaaatt
      781 agatactaaa gaaatcctaa ttgagactgt taaggaagca atccctttta ttattactgg
      841 tgcagcaatt caaattttca aattgattga tcagttttca tttgggaata cgatggcgct
      901 ctttacgaat tattcaagtg aagaattacg ggttatgttt gcttactttt caagcaaccc
      961 tggtaaagtt acgatgattc taattgctgt tgctaccgca attgctggag ttggtattcc
     1021 attgttaaca gagaattttg ttaagaatga taaaaaagcc gccgctcgtt tggttgttaa
     1081 taatttacaa atgttactaa tgtttttact gccagctgtt gcaggatccg ttattttagc
     1141 aaaacctttg tatacagtct tttatgggtt accacaggga caagctttag ggctttttgt
     1201 catatctctt attcaaacaa ttattttatc tatttatact gttttagcac caatgctaca
     1261 agcattgttt gaaaatcgta aagctattat ttacttccta tatggtctgg tggcaaaagt
     1321 tattttacaa gttccctcca ttttcctttt ccatgcctat ggaccacttt tctcaacgac
     1381 agtagctctt tgtatcccag taattcttat gtatttgaaa atacatgaaa ttacaggatt
     1441 taaacgccaa gctattcgtc gtacaagtgc tttagtcctt attctaacgc tattgatgag
     1501 ctttattatt tctatgataa tatggttaat gaacctggtt attgtacccg atagtagact
     1561 ggtaagtcta gtttacatta tcgttatcgg tgcaataggt ttaggggtat atggctttat
     1621 ggcactggcc acccatctac ttgataagat gattggtagt cgtgctcaag acttacgccg
     1681 aaagttgcac cttaactaat gaaaaaatag gttagacaga attgtttaac ctagtttttt
     1741 gacgaccaaa attatttgtg aaattttaag gtagtcacaa tatagtgagc ttatttttat
     1801 gttataatta aatggagaaa tatgaggtga aagatgtctt acgaaaagct tttagaacga
     1861 tttttaacat acgtcaaaat aaatacaaga agtaatccta atagtacaca aacgccaaca
     1921 actcaaagtc aagttgactt tgctttaaca gttttaaaac cagaaatgga agcaattggt
     1981 ttaaaagatg ttcattattt accttctaat gggtatttgg ttggaacctt acctgctaca
     2041 agcgaccgct tacgccataa aataggtttt atatcccata tggatacagc tgatttcaat
     2101 gctgaaaata ttactccaca aattgttgac tataaaggtg gagatattga acttggagac
     2161 tcaggttaca ttttaagtcc gaaagatttt ccaaatttaa ataattacca tgggcaaaca
     2221 ttgattacaa cagatggtaa aaccttactg ggagcagacg ataagtctgg tatagcagaa
     2281 atcatgacag ctatggaata tttggcttcg catccagaaa ttgagcactg tgaaattaga
     2341 gttggctttg gaccagatga agaaattggt ataggtgcag ataaatttga tgttaaagat
     2401 tttgatgttg attttgccta tacagtggat ggtggaccac taggagaatt acagtatgaa
     2461 acctttagtg cagctggttt ggagcttaca tttgaaggac gaaacgttca ccctggaact
     2521 gcaaaaaatc aaatgattaa tgctttacag cttgctatag attttcatag tcaattacca
     2581 gaaaatgaac gtcctgaaca aacagatggc tatcaaggat tttatcactt atatgattta
     2641 agtggaacag ttgatcaagc taaaagttca tatatcattc gagattttga ggaagttaat
     2701 ttcttaaaac gtaagcaatt ggctcaagct atcgctgata atatgaatga agcattacaa
     2761 tctgaacgtg taaaggttaa actatacgat caatattaca acatgaagaa agttattgaa
     2821 aaagacatga cacctatcaa cattgctaaa gaagtaatgg aagagttaga catcaagccg
     2881 atcatagaac cgattcgtgg tggtacagat ggctctaaaa tttcctttat gggaatccct
     2941 actcctaatc tttttgcagg tggtgaaaac atgcatggac gctttgaatt cgttagtcta
     3001 caaacaatgg aaaaagcagt tgatgttatt ttaggcatcg ttgctaagga ttagtaaaga
     3061 aatatcagat tggagtggtt aaatgataaa attatttggg aagatacgtt atcactggca
     3121 acctgaactt tcttgggcta ttatttattg gtcaatagcc attgcaccaa tttttattgg
     3181 tttatctctg ctatatgaaa gaactgagat tccgagtcag gtatttgttc tatttgccat
     3241 ctttattgtt ttagtaggca ttggatttca tcgctatttt gttattgaag aagatggata
     3301 tcttaggatt gtatctttta actttttaag acgatctaag tttccaattg aagatattgc
     3361 taaaattgag gtcactaaat caagtgtcac tatcaaattt aataataacc atgagcgtat
     3421 tttttacatg cgaaaatggc ctaaaaaata ttttttagat gcactagcta ttgaacctaa
     3481 atttaaggga gaagtggagc ttctcgataa tttgattaaa atggattatt ttgaatgtta
     3541 tcgctacgat aaaaaagctc ttacaaaatc gtaagagctt tggtgggaca tgatttaact
     3601 gctaaaacag tatcttgatc agaacttgga atttcttttt ctaggttatc tgtatcggaa
     3661 aatttaacga taccatcatc ttgataatca aaaatatttg aatatgtttg gcaaagcccg
     3721 caggcaatac atttttctgg aattagtgca actttcatag tattattgta atacttattg
     3781 atacgattaa caagggggaa gtcatggcga aaaaaccatg ggaaaaaaaa gttgtggaaa
     3841 acaatagtca tagaaaagac aaaattacaa gaacgagtag aggtgtggtg tcaagtactc
     3901 cttggattac cgctttcttg agtgcattct ttgttattgt cgttgctatt ttatttatag
     3961 tattttatac ttcaaataga ggagaagaca gagcaaaaga aacttctggt ttttatggtg
     4021 cttcaagtca aaaagttaat agttctaaaa cgaaaaaagc atctacaagt aagaaaacaa
     4081 cgtcatcatc aaaaactaaa actagtgata aatcaacaac atcttctagt acagtagcaa
     4141 gttcgagtga gacatcgaca tcatcttcag taaacggtgg aacaatcgta gttcaaccag
     4201 gtgagggagc agcctccata gctgctcgtg caggtatctc agttgctcgt ttacaagaac
     4261 ttaatcctgg tcatatgacc tcagggtatt ggtatgctaa tccaggcgat cctgtggttg
     4321 taaattaagg ggaggattat gaactcaatt aatattgcta ttgatggacc agcatcaagt
     4381 gggaaaagta ctgtcgctaa gattattgct aaaaatttga attacactta ccttgataca
     4441 ggtgctatgt atcgttgtgc tacttatctg gctttacagc atggatatga agcacaagat
     4501 gtttcgaaaa ttttaggact cctagctgaa agaccgattt cttttggtaa agcagaagat
     4561 ggcagccaga cagtctttct tggcacagaa gaagttactt tagctatccg tcaaaacgat
     4621 gttactaaca atgtctcgtg ggtttctgct atcccagaga ttcgagaaga gttagtaaac
     4681 cagcagcgtc gtatagccaa agatggtgct attataatgg atggtcgtga cataggaaca
     4741 gttgttttgc cagatgcaga attgaagatt tttttagtgg cttctgttga tgaaagagct
     4801 gagcgtcgct ttaaagaaaa tcaagaaaaa ggcattgaga gtgattttga aacactgaaa
     4861 tcagaaattg ctgctcgtga ttataaagat agccatcgag aagtttcacc attaaaggca
     4921 gcagaagatg ctattgaatt tgatactaca ggagtatcaa ttgaaggtgt tgtaactttt
     4981 attcaagaaa aagcagaaaa aataattgac atgaagaatt agtcatgata tactagttgc
     5041 aatgagaaaa gcagaagtga gaacttctcg ccttgcgact taggttgtct ggcccaacat
     5101 gtcaagattc catctaggaa gattatatgt gcgggcttga tttatcaagt ccgctttcgt
     5161 ttttctctaa aaaaataaag aggtgaagat catagctaaa aaagatctat tcattaacga
     5221 tgaaattcgc gttcgtgaag ttcgtctggt tggtctcgag ggtgaacaat taggtattaa
     5281 accattgtct gaagctcaag caattgcaga tgatgctaat gttgatttgg ttttaattca
     5341 gccacaagct acaccacctg ttgcgaaaat tatggactat ggtaagttca aatttgagta
     5401 tcaaaagaaa caaaaagaac aacgtaaaaa acaaagcgtt gttactgtta aggaagttcg
     5461 tcttagccca gttattgata aaggggattt tgaaacaaaa cttcgtaacg gtcgtaagtt
     5521 ccttgaaaaa ggtaacaaag ttaaagtttc tatccgattc aaaggacgta tgattactca
     5581 taaagaaatt ggagcaaagg tattggcgga atttgctgaa gcgacacaag atatcgctat
     5641 tattgagcaa agagctaaga tggatggtcg ccaaatgttt atgcagcttg caccaattcc
     5701 agacaagaaa taattgtcga ctttatagta ttatttaaga ggagaattta aaatgccaaa
     5761 acaaaaaaca caccgcgcat cagctaaacg ctttaaacgc acaggttcag gtggattgaa
     5821 acgcttccac gcgtttactt cacaccgttt ccacggtaaa actaaaaaac aacgtcgtca
     5881 tcttcgtaaa gctactatgg tttcatcagg agatttcaaa cgtattaaag caatgcttac
     5941 tcgccttaaa taataaggtc tctgtaaaca tttactacat attataaagg attatttgga
     6001 ggaaatataa atggctcgtg ttaaaggtgg agttgtttca cgtaaacgtc gtaaacgcgt
     6061 acttaaatta gctaaaggtt actatggtgc aaaacatatc ttgttccgta ctgcaaaaga
     6121 acaagtaatg aattcttact actatgcata ccgtgaccgt cgtcagaaaa aacgtgactt
     6181 ccgtaaatta tggatcactc gtatcaatgc ggctgctcgt atgaatggtt tatcatattc
     6241 acaattgatg cacggtttga aattagctga aatcgaagtt aaccgtaaaa tgcttgctga
     6301 tttagcagtt aacgatgcag cagctttcac agctcttgca gatgcagcta aagctaaact
     6361 tggtaaataa gaaataaaaa agttgggtct gcctaacttt ttttaattac tagaaagaaa
     6421 gtacctatat tataatttaa gtgaaaagtt tcgttctata tatgtagaat gaaacttttt
     6481 ttgttttatt ccgatacctt tattattgtt ataattttcc tttttgattt ttattgtttt
     6541 atagtattat tccgtataaa aagcacctca atcgttggat tgaggtgtgg attaaacttt
     6601 aaatggtaaa gattattttt tagatgtttt atgtgtctta ttaggatctt gcaattccaa
     6661 gtaacttgga gcgtggaaga gatcaactgt tgatttgttg ttatgtttgc tgtatatgct
     6721 tgttgaacga tctttcaatt tacgttcttt agctttgagt gctttaagag aatgtgtgta
     6781 attgaatttt tcaacttcta cagttttcaa accattgttt tcatcaaatc gtagtaaatc
     6841 tccagtttga atcgaatcac ttgtagataa ttgtttatta gtagcatctc gaatagtttt
     6901 tatttcagct ttagttgttt catcaggatt tgtgatttct tgtcctgaat cagtgtaata
     6961 gagatgtcca ctatagttag tgtatttagg agtaatatac tgaccagttg ttctcatagc
     7021 gaccatttgt ttattatctt tagataacaa gtcttgtcct aactgagtat atttagacgt
     7081 gtctatccct agaatatgaa gtaaggttgg taaattatct acctcaccac cgtatgtatt
     7141 actgataaaa ccttttgaat agccaggaat gtggatcata tatggaacac gttgtaacat
     7201 agcgttgtcg tattcagacc atgtttcagg gtctttacct aataattcag ctaagctagg
     7261 attgcgtgta ttagaaatac cgtagtgatc cccatacatt acaataatag aattatcata
     7321 cacaccagca gcttttagat attcaaaaaa tgatttaaga gctgtatcaa ggtagtttgc
     7381 tgtggcgaaa taaccgttaa tcgtttcatc gttagtcttt gcaagtggga atccctcttc
     7441 gtcagactct cccttcaaac ttgtgtaagg gtaatggtta cttactgtaa taaatttagt
     7501 ataaaacggt tgttgcatgt gctctagata tttgatagag tcagcaaaca tatatttatc
     7561 attcaaaccg tattggaatg aattatcttt ggtttgtttt gagaagtagc tagagtcaaa
     7621 gaagtagtca taaccccact gtttgtaagc attatttcta ttccaaaatg ttccgacgtt
     7681 tccgtggaag accgcactac tgtaacctcc attttgagct aagatatgtg gagcagcaaa
     7741 ttgtgtattt tcaccaccat aattaaccat gaatgaacca ctacttagac caaaaagtga
     7801 gttttccatg agtgtctcag catcggatgt cttaccagct ttgacttgat ggaagaagtt
     7861 agagaaggag atagtctctt tcgaatgata aaggctgtta ataaatggag taaccacatg
     7921 ttctttacca tcaacattta atttatagtc aatcaagaat tgttgaaaac tctccaaatg
     7981 aatcatgata acgtttcgcc cttttccgat accataatat tctggattgg gtttggcata
     8041 atgctctgct acatattttt tggcagtcgc taattcttgt gcagtggcac catttctttc
     8101 tttctgtgct tgataagttt gattaccact ataaatcgaa aatgatggca agccaagagc
     8161 tttaacaata taggtgtttg aaaaaccacg acttagtagt tctggacgat caatttcagc
     8221 taagaataag ttaattgaaa agagtaaacc agacaaagct gtgatagaaa aactagcacg
     8281 tttattaaat ggacgattat ctaagtggat tttcttggtt gcaaataaga aaataaggat
     8341 aatgaaatcg aaaacgtaga caagatccca aattctcaat agattaagag cagagtctcc
     8401 aaggccagcc gaagttttac tactagccaa gacagcgcta actgtaatga aatcggaaaa
     8461 ctctctatag tagattgcgt ttgcaattag aaggatattt acgatagcat aggtgataaa
     8521 agcggtaata taaaaagctt tggttcgctt aacatataag gccaaaccta tgattaagag
     8581 tcctaagggg atggggttta taatggtcaa aaacaactga taggaatttt ctaaacctaa
     8641 attaaaatca gtatggt
//
LOCUS       DATRQA010000082         8616 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.082, whole genome shotgun sequence.
ACCESSION   DATRQA010000082 DATRQA010000000
VERSION     DATRQA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8616
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.082"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            176..1372
                     /gene="tuf"
                     /locus_tag="VBD27_001386"
     CDS             176..1372
                     /gene="tuf"
                     /locus_tag="VBD27_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO7025392.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPTSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVLAKPGSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            1553..2311
                     /locus_tag="VBD27_001387"
     CDS             1553..2311
                     /locus_tag="VBD27_001387"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO7025393.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFIEAVASKLPSSELVEAGIAAP
                     ALTLSTVLEAAKGSELKIAAQNSYFENSGAFTGENSPKVLAEMGTDYVVIGHSERRDY
                     FHETDQDINKKAKAIFANGLTPIICCGESLETYEAGKAVEFVGAQVSAALAGLSEEQV
                     SSLVIAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQAVADKVRVQYGGSVKPE
                     NVAEYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            2488..3180
                     /locus_tag="VBD27_001388"
     CDS             2488..3180
                     /locus_tag="VBD27_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEO7025394.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIQ
                     AAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPDMAKDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFEFDE
                     KLNLVSEYYLGK"
     gene            3309..5354
                     /locus_tag="VBD27_001389"
     CDS             3309..5354
                     /locus_tag="VBD27_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO7025395.1"
                     /translation="MLNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQM
                     QIGQQSFYMKKLTALTSYTVKESKARGQIFDAKGVVLVENDERPTVAFSRGNNISSQS
                     IKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGNHLAES
                     TVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDAD
                     KKELLGISVTSNWHRRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTS
                     YLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNITQEGKSGRNLKLTIDLNYQNKVESIL
                     KQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTIAKNFTP
                     GSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMV
                     QVALRLMGQDYNTGDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYS
                     LGTTLMESFGQYDAYTPMQLGQYISTIANNGNRLAPHVVSDIYEGNDSNKFAQLVRSI
                     TPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGTAETFAKN
                     INGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTTKSHQLVARDMIDQYISQFTGQ"
     gene            5369..5965
                     /gene="recR"
                     /locus_tag="VBD27_001390"
     CDS             5369..5965
                     /gene="recR"
                     /locus_tag="VBD27_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO7025396.1"
                     /translation="MLYPTPIAKLIDSFSKLPGIGTKTATRLAFYTIGMSDEDVNEFA
                     KNLLAAKRELTYCSVCGNLTDDDPCLICTDKTRDQSVILVVEDSKDVSAMEKIQEYNG
                     LYHVLHGLISPMNGISPDDINLKSLITRLMDGQVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            6106..7152
                     /locus_tag="VBD27_001391"
     CDS             6106..7152
                     /locus_tag="VBD27_001391"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO7025397.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIT
                     QVGQFIKTQEFDEMPSSDEKLMTNQTVDLDKMVRPSDIYDDNAIVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKQVLATVGVPQVAYQTYFEGDDLEHAIKL
                     SLETLSFPIFVKPANMGSSVGISKATDESSLRSAIDLALKYDSRILIEQGVTAREIEV
                     GILGNNDVKTTFPGEVVKDVDFYDYDAKYIDNKITMDIPAKVDEATMEAMRQYASKAF
                     KAIGACGLSRCDFFLTKDGQIFLNELNTMPGFTQWSMYPLLWENMGLTYSDLIEKLVM
                     LAKEMFEKRESHLI"
     gene            7299..>8616
                     /locus_tag="VBD27_001392"
     CDS             7299..>8616
                     /locus_tag="VBD27_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO7025398.1"
                     /translation="MKLSLHEVAKVVGAKNQVSEFEDVPLGNIEFDSRNISEGDLFLP
                     LKGARDGHEFIEMAFDNGAIATISEKEIEGHPYLLVSDALKAFQVLAQYYIEKMNVDV
                     IAVTGSNGKTTTKDMIAAILSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIVLEMG
                     QDHLGDIHVLSEIAKPRIAVVTLIGEAHLEFFGSREKIAEGKMQITDGMSSDGILIAP
                     GDPIIDPYLPANQMTIRFGHDQELQVTELKEEKHSLTFKTNALEHQLRIPVPGKYNAT
                     NAMVAAYVGKLLAVAEEDIVDALENLQLTRNRTEWKKSANGADILSDVYNANPTAMRL
                     ILETFSAIPNNDGGKKIALLADMKELGEQSVDLHNQMIMSIRPDSIDTLICYGQDIEG
                     LAQLASQMFPIGKVYFFKKNQEVDQFDQLLAKVKDTLKEKDQILLKG"
BASE COUNT         2875 a         1415 c         1743 g         2583 t
ORIGIN      
        1 gagataacaa acgcctcaat caatcttacc cgaattttaa ggaaataaag aaataagcaa
       61 aaaagcataa aatacttgct tttttagagg attttgttaa aatagataag taaagttaga
      121 ctgtattgcc taccgtctat ctataaaata tattttattg gaggcttttc ctaaaatggc
      181 aaaagaaaaa tacgatcgta gtaaaccaca cgttaacatt ggtacaatcg gacacgttga
      241 ccacggtaaa actactttaa cagcagctat cacaactgta ttggcacgtc gcttgcctac
      301 ttcagttaac caaccaaaag attacgcttc tatcgatgct gctccagaag agcgcgaacg
      361 cggaatcaca atcaacactg cacacgttga gtacgaaact gaaaaacgtc actatgccca
      421 catcgacgct ccaggacacg cggactacgt taaaaacatg attactggtg ctgctcaaat
      481 ggacggagct atccttgtag ttgcttcaac tgatggacca atgccacaaa ctcgtgagca
      541 catccttctt tcacgtcaag ttggtgttaa acaccttatc gtattcatga acaaagttga
      601 ccttgttgat gatgaagaat tgcttgaatt ggttgaaatg gaaattcgtg accttctttc
      661 agaatacgac ttcccaggtg atgaccttcc agttatccaa ggttcagctc ttaaagcact
      721 tgaaggcgat gaaaaatacg aagacatcat catggaattg atgagcactg ttgatgagta
      781 cattccagaa ccagaacgtg atactgacaa acctttactt cttccagtcg aagatgtatt
      841 ctcaatcact ggacgtggta cagttgcttc aggacgtatc gaccgtggta ctgttcgtgt
      901 caacgacgaa gttgaaatcg ttggtattaa agaagatatc caaaaagcag ttgttactgg
      961 tgttgaaatg ttccgtaaac aacttgacga aggtcttgca ggggacaacg ttggtgttct
     1021 tcttcgtggt gttcaacgtg atgaaatcga acgtggtcaa gttcttgcta aaccaggttc
     1081 aatcaaccca cacactaaat ttaaaggtga agtttacatc ctttctaaag aagaaggtgg
     1141 acgtcatact ccattcttca acaactaccg tccacaattc tacttccgta caactgacgt
     1201 aacaggttca atcgaacttc cagcaggaac agaaatggtt atgcctggtg ataacgttac
     1261 tatcgaagtt gaattgattc acccaatcgc cgtagaacaa ggtactactt tctcaatccg
     1321 tgaaggtgga cgtactgttg gttcaggtat cgtttcagaa atcgaagctt aatttaactt
     1381 aattaagttc ccaaacggca gttagagtca gacagctcaa attgtgaaga gtcctcaggg
     1441 actctttttt agtatctata attaaagcgt tttactagac tcgtgaaact tttaattaac
     1501 tataatttat ggtatgatag attagtaaat aaaaaaagaa gaggtatgtg taatgtcacg
     1561 taaaccattt atcgctggta actggaaaat gaataaaaat ccagaagaag caaaagcatt
     1621 catcgaagca gtagcttcta aattaccatc atcagaactt gttgaagcag ggattgctgc
     1681 tccagcactt actttatcaa cagttcttga agcagctaaa ggttcagaat tgaaaatcgc
     1741 tgctcaaaat agctattttg agaattcagg tgcattcact ggtgaaaaca gccctaaagt
     1801 attggctgaa atgggtacag actacgttgt tatcggtcac tcagaacgtc gtgattattt
     1861 ccatgaaact gaccaagata tcaacaaaaa agcaaaagct atctttgcta atggtttgac
     1921 tccaatcatt tgttgcggtg agtctcttga aacttacgaa gctggtaaag cggttgaatt
     1981 cgtaggtgct caagtttcag cagctcttgc aggtttgtca gaagagcaag tatcatcact
     2041 tgttatcgct tacgaaccaa tctgggctat tggtactggt aaatcagcaa ctcaagacga
     2101 cgcacaaaac atgtgtaaag cagttcgtga cgttgttgca gcagacttcg gtcaagcagt
     2161 tgctgacaaa gttcgcgttc aatacggtgg ttcagttaaa cctgaaaacg ttgcagaata
     2221 tatggcttgc ccagacgttg acggtgccct tgtaggtgga gcttcacttg aagcagaaag
     2281 cttccttgcc ttattagatt ttgttaaata aatcacatga tatctaaagg catagcactc
     2341 taggtgatat gccttttctt atagatagtt aataataagc tatttttgat tattgaacta
     2401 gtaatcagac tagattttta tggctatttt ttgtagaata gttaaagtaa atattggtat
     2461 agccaaaatt aaaggagaaa ttttctaatg gtaaaattag tattcgcacg ccacggtgaa
     2521 tctgagtgga ataaagctaa ccttttcact ggatgggctg acgtagatct ttcagaaaaa
     2581 ggtacacaac aagctattga tgctgggaaa ttaattcaag cagcaggtat tgagttcgac
     2641 cttgctttta catcagttct taaacgtgcc atcaaaacaa ctaaccttgc ccttgaagca
     2701 gctgatcaac tttgggtacc agttgaaaaa tcatggcgct tgaacgaacg tcattacggt
     2761 ggattgacag gaaaaaataa agcagaagca gctgaacaat ttggtgatga gcaagttcat
     2821 atttggcgtc gttcatatga tgtattgcct ccagatatgg ctaaagatga tgaacattca
     2881 gcacatactg atcgtcgcta tgcttcacta gatgattctg ttattccaga tgcagaaaac
     2941 ctaaaagtta ctttagagcg tgctcttcct ttctgggaag ataaaattgc tcctgctctt
     3001 aaagatggta aaaatgtgtt tgttggtgca cacggtaact caatccgtgc tcttgtaaaa
     3061 catatcaaac aattgtcaga tgatgaaatc atggacgttg aaattcctaa cttcccacca
     3121 cttgttttcg aatttgatga aaaattaaac cttgtttcag aatattactt aggtaaataa
     3181 tctaatcttt atataaaaaa ttcaggccaa gttggcttga attttttatt atttataaca
     3241 ttttgagtgt ctgtaaaaaa ccatttttat gatacaatag tagtcagagt ttttatagca
     3301 aggagttaat gttgaatcgt aaaaaaaggt atcgtctcac tgtaaaaaag caaaatgcta
     3361 gcatacctag acgattaaat cttctatttt ttattattgt tttattattt actgttctaa
     3421 ttcttcgttt agagcaaatg cagattgggc aacaatcatt ttacatgaaa aaactgacag
     3481 ctcttacgag ttatacagtg aaagaatcaa aagcgagagg tcagattttt gatgctaagg
     3541 gagttgtctt agttgaaaat gatgaacgtc ctactgtggc gttttctaga ggaaacaata
     3601 tttcatctca gtctatcaaa gagttagcga ataaattatc acattacatt accttaaccg
     3661 aagttgcttc aagcgataga gcaaagagag attattattt agcagataaa gccaactaca
     3721 aaaaagttgt tgaaagttta ccagattcta aacgttacga taaatttggt aaccatctag
     3781 ccgaatcaac cgtatatgca aatgcagttg ctgcagttcc agtgagcgct attaactatt
     3841 ctgaagatga gttaaaagtg gttgccttat ttaaccaaat gaatgcgaca ccaacgtttg
     3901 gttcagtgaa attaagtaca ggtgaattaa gtgatgatca aatcaagaaa ctggatgctg
     3961 ataagaagga actgttagga atttctgtaa caagtaattg gcatcgtcgt aaaaaaggaa
     4021 cttctttatc ggatatatta ggtactattt ctacagaaaa ggcaggttta ccaagagaag
     4081 aagttaaaaa atatttgaaa aaaggctatt cactgaatga ccgagtggga acttcttacc
     4141 ttgaaaaaca atatgaagat gacttacaag gaatcagaca aattcgcaaa gtagttgtta
     4201 ataaaaaagg taaagttgtt tcagataata ttacgcaaga aggtaagtca ggaagaaatc
     4261 ttaaattaac gattgatctt aattaccaaa acaaagtaga aagtatcttg aaacaatact
     4321 atggcagtga attatctagt gggcgcgcta gtttttccga agggatgtat gcagttgcta
     4381 tagaaccttc aactggtaaa gtgttagcaa tggctgggct gaaaaatgat catgggaatt
     4441 tagttgatga cagcctaggc actattgcta agaatttcac gccaggatct gttgtgaaag
     4501 gtgcgacgct atcatcagga tgggaaaata aagttcttag aggaaatgaa gtgctttatg
     4561 accaagaaat tgctaatata cggtcatggt ttacacgagg tttgactcca atatctgcgg
     4621 cacaagcgct agagtattca tctaatactt atatggttca agtagcactt cgtttaatgg
     4681 gacaagacta taatacagga gatgctttaa ctgatcgagg ctatcaagaa gcaatggcta
     4741 aactaaggaa aacttacggc gaatatggtt taggggtttc tacaggatta gatttacctg
     4801 aatcagaagg ttatgtacct ggaaaataca gcttaggaac aactctaatg gaatcgttcg
     4861 gtcagtatga tgcctataca ccaatgcaac ttggtcagta tatctcaact attgcgaata
     4921 atgggaatcg tttagcacct cacgtggttt cagatatcta tgaagggaat gattctaata
     4981 agttcgctca attggttcgt tcaatcactc ctaaaacact aaataagata gctatctcag
     5041 atcaagagtt agccattatt caagaaggtt tttataacgt tgtcaatagt ggaagtggct
     5101 atgcaactgg tacgtcaatg agggggaatg tgacaaccat tagtggtaaa actggtaccg
     5161 ctgaaacatt tgctaaaaat ataaatggac aaacagtttc tacctacaac ttaaacgcta
     5221 ttgcctacga tactaatcgt aaaatagcag tagcggtaat gtatccgcat gttacaactg
     5281 atacaacaaa atcccatcaa ttagttgcac gagatatgat tgatcaatat atttcacagt
     5341 tcacaggaca ataagagagg acatttgaat gctttaccca acaccaattg ctaaattgat
     5401 tgacagcttt tcaaaattac cgggtatagg tactaaaaca gcaacacgtt tagcttttta
     5461 caccattggt atgagtgatg aagatgttaa tgaatttgct aagaatttat tagctgctaa
     5521 gcgggaatta acctattgtt ctgtttgtgg taatttgact gatgatgatc cttgcttaat
     5581 ttgtacagat aagacgcgtg accagtctgt tatcttggtt gttgaagata gtaaagatgt
     5641 gtcagctatg gaaaaaattc aagagtataa tggtttgtat catgtcttac atggcttaat
     5701 ttcacctatg aatggtatta gccctgatga cattaatttg aaaagtttaa ttactcgatt
     5761 gatggatggt caggtgacag aagttattgt tgctaccaat gcgactgctg atggtgaagc
     5821 tacatcaatg tatatttcac gagtcttaaa accagctggt atcaaagtga cacgattagc
     5881 gagaggttta gctgttggtt cagatatcga atatgcagat gaagtaacct tgcttcgagc
     5941 aatcgaaaat cgtacagagt tataattgtt ataaagaaaa ggttgggaat ctcctaactt
     6001 tttcttttac aatactaact ttaagtggat tgatttttga aagttaagtt aaaatatggt
     6061 attattaaaa ggtcaatctt agattagata gaaaagagaa gaaatatgtc aaaagaaaca
     6121 ttgatcttgc tttacggtgg tcgctctgcg gaacgtgaag tatctgtact gtctgcagaa
     6181 agcgtcatgc gtgctattaa ttatgataaa ttttttgtta aaacttattt tatcacgcaa
     6241 gtaggtcaat ttattaaaac acaagaattt gatgaaatgc catcttcaga tgaaaagtta
     6301 atgacaaacc aaactgttga tttagacaaa atggttcgtc caagtgatat ctatgatgat
     6361 aatgcaattg ttttccccgt tttacatgga ccaatggggg aagatggttc tatccaagga
     6421 tttttagaag ttttaaggat gccttatgtt gggactaata ttctatcttc aagcgtggct
     6481 atggataaaa ttacaacaaa acaagtcctt gcaacagtag gtgtacctca ggttgcatat
     6541 caaacttatt ttgagggtga tgatttggaa catgcgatta aactctcttt agaaacttta
     6601 agtttcccaa tttttgtaaa accggctaat atggggtcat cagtaggtat ttcaaaagcg
     6661 acagatgaat cctcacttcg ctctgcaatt gacttagctc tcaagtatga tagccgtatt
     6721 ttgattgaac aaggcgtgac agctcgtgaa attgaagtag gtattttagg caataatgat
     6781 gttaagacaa cttttcctgg cgaagttgtt aaagacgtcg atttctatga ctatgacgcc
     6841 aaatatattg ataataaaat tactatggat attccagcta aagttgatga agcaactatg
     6901 gaagcaatgc gtcaatatgc aagtaaagct tttaaagcaa tcggggcttg tggtttatca
     6961 cgctgtgatt tctttttgac gaaagatgga caaatcttct taaacgaact gaatacaatg
     7021 cccggtttta ctcagtggtc aatgtatcct ctgctttggg aaaatatggg gctaacttat
     7081 agtgatttga ttgaaaagtt agttatgtta gctaaagaaa tgtttgaaaa acgtgagagt
     7141 catttaattt aaaaaagttc aactaagtct ttcttagttg agctttttca aatctatgat
     7201 atgttatgaa agtgtgttat aatttaggag tatattcgtt tataagggat agcatactat
     7261 ctacaacatt gttgctaaat aatagaaaag gaaatgatat gaagcttagt ttgcatgaag
     7321 ttgctaaagt agttggcgct aaaaatcaag tatcagagtt tgaagacgtt cccctgggaa
     7381 atattgaatt tgatagtcgt aatatcagtg aaggtgactt atttcttccc ttaaaaggag
     7441 caagagatgg acatgagttt atagagatgg cttttgataa tggagcaata gcaaccattt
     7501 cagagaagga aatagagggt catccttatc ttttagtgtc tgatgcactt aaagcatttc
     7561 aagtcttggc acagtattat attgaaaaga tgaacgttga tgttattgca gtaactggct
     7621 caaatggtaa gacaacaact aaagatatga tagctgcaat cttatcaacc acatataaga
     7681 cttataaaac acaaggaaac tacaataacg agattggctt accatatact gtgctacaca
     7741 tgccagaaga tactgaaaaa attgttttgg agatgggaca ggatcatctt ggagatatcc
     7801 atgtgttatc tgaaattgca aaaccacgta ttgctgttgt tactttgatt ggtgaagcac
     7861 acttagaatt ttttggtagt cgagaaaaga ttgcagaagg gaaaatgcaa atcacagatg
     7921 gtatgagttc agatggcata ttaattgcac cgggagatcc aattattgat ccatatcttc
     7981 cagcaaacca gatgactatt cgttttggcc atgaccaaga attgcaagtg acagaactca
     8041 aagaagaaaa acattcctta acttttaaaa ctaatgcttt ggagcaccaa ttacgtatac
     8101 cagtacctgg taagtacaat gcaactaatg cgatggtcgc tgcctatgtt ggaaaattat
     8161 tagcagtagc agaagaagat atagtggatg ctctagaaaa tcttcaacta actcgtaatc
     8221 gcaccgaatg gaagaaatca gccaatggag cagatatttt atcagatgtt tacaatgcta
     8281 acccaacggc tatgcgattg atactagaaa ccttttcagc tattcccaat aatgatggtg
     8341 gaaagaagat tgctctttta gctgatatga aagaactggg agaacaatct gtggatttac
     8401 ataatcaaat gattatgagt atcagacctg actctattga tactttgatt tgttatggcc
     8461 aggatattga ggggttagct caattagcaa gccagatgtt cccaattggt aaagtgtatt
     8521 tctttaaaaa gaatcaagaa gtggatcagt ttgatcaatt gcttgcaaaa gtaaaagata
     8581 ctcttaagga gaaagatcaa atattattaa aaggtt
//
LOCUS       DATRQA010000083         8594 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.083, whole genome shotgun sequence.
ACCESSION   DATRQA010000083 DATRQA010000000
VERSION     DATRQA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.083"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(146..703)
                     /gene="lepB"
                     /locus_tag="VBD27_001393"
     CDS             complement(146..703)
                     /gene="lepB"
                     /locus_tag="VBD27_001393"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO7025399.1"
                     /translation="MVKRDFIRNIILALIAVLILILLRYFVFATFKVHKDATNSYFSN
                     GDVVVVNRNRTPKYKDFIVYKVGKIFYISRVIGEPNQKVRVMDDILYLNDVFKDEPYI
                     EKMKNAYSEKKDGQMPFTSDFSVETLTRNKESRVPKGSYLVLNDNRQNKNDSRKFGLI
                     KEKDIRGVITFKVYPLSEFGFTASE"
     gene            complement(874..2376)
                     /gene="pyk"
                     /locus_tag="VBD27_001394"
     CDS             complement(874..2376)
                     /gene="pyk"
                     /locus_tag="VBD27_001394"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO7025400.1"
                     /translation="MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQ
                     LIKEGANVFRFNFSHGDHAEQGARMATVRKAEEIAGQKVGFLLDTKGPEIRTELFEDG
                     ADFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDGKLGLTV
                     FAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVNEVRAICEETGNGHVKLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTDKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFPRNNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLAD
                     KPASTDDMFEVAERVALEAGLVESGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            complement(2425..3447)
                     /gene="pfkA"
                     /locus_tag="VBD27_001395"
     CDS             complement(2425..3447)
                     /gene="pfkA"
                     /locus_tag="VBD27_001395"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO7025401.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINQGYYGM
                     VTGDIFPLDANSVGDTINRGGTFLRSARYPEFAELEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDYTIGFDTAVATAVENLDRLRDTSASH
                     NRTFVVEVMGRNAGDIALWSGIAAGADQIIVPEEEFNIDEVVSNVRAGYAAGKHHQII
                     VLAEGVMSGDEFAKTMKAAGDDSDLRVTNLGHLLRGGSPTARDRVLASRMGAYAVQLL
                     KEGRGGLAVGVHNEEMVESPILGLAEEGALFSLTDEGKIVVNNPHEADLRLAALNRDL
                     ANQSSK"
     gene            complement(3528..6632)
                     /locus_tag="VBD27_001396"
     CDS             complement(3528..6632)
                     /locus_tag="VBD27_001396"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO7025402.1"
                     /translation="MFAQLDTRTVYSFMDSLVDLKTYVSKSKSLGYQTIGILDHSNLY
                     AAYHFIQEAQKANLRPIVGFSFDIVVENRSIEVYCIAINTVGYKNLLKLSTAQMSEKM
                     SLNLLTEHLEGVQLILPYQDALDQLNLPFDYVIGVNLTSPQIPYTKPIIAIDTVRYFQ
                     KNDIETLQMLHAIRDNVLLKDAQYASKNQELKPCQEMTLAFKNRFPEALANLESLVEN
                     VVYHFDSDFKLPIFNREIPAGEELRTLTQNNLKSKGLWSDAYQERLEKELTIIHKMGF
                     DDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVSYALNITGIDPVKHNLIFERFLNEE
                     RYSMPDIDIDLPDIYRGEFLRYVRNRYGSMHSAQIVTFSTFGAKQAIRDVFKRFGASE
                     YELTNITKKIHFRDNLTSVYNRNLAFRQIIDSKIEYQKAYDIAKRIEGNPRQTSIHAA
                     GVVMSDDLLTDHIPLKNGEDMMITQYDASSVEANGLLKMDFLGLRNLTFVQKMKEKVD
                     KDYGISIQLEAIDLEDKETLKLFAAGQTKGIFQFEQSGAINLLRRIRPECFEDVVATT
                     SLNRPGASDYTENFINRRFGKEKIDLVDPVIAPILQPTYGIMLYQEQVMQIAQTYAGF
                     TLGKSDLLRRAMSKKNSKEMQKMSQSFLEGAVSKGHRQEDARLIFERMAKFAGYGFNR
                     SHAFAYSALAFQLAYFKAHYSDVFYDIMINYSNSDYLIDAIDFGFVIEKPSINTISYR
                     DRIYKKKIYLGLKNIKAVPNDLAYWISKNQPFQSIEDFLMRLPQQFQKSGFISPLIAI
                     GAFDEFDNNRRKITSNLDSLFTFVNELGSLFADTSYHWLEVEDFSNSEKYEMEQDILG
                     VGISPHPLVGISQKASRPFIPISQVQENSEATILVQLKQVKIIRTKSSGQQMAFLTVM
                     DINSKMDITVFPETFNIVRDDLQEGKYYYLHGKIQKRDERLQMVLNGVQEATEERFWI
                     LLKNHDNDKKISEILSKYKGHIPVYLHYETTKETIQSKVHLVRKDSGLALDLSEFVVK
                     TVYQ"
     gene            6777..7145
                     /locus_tag="VBD27_001397"
     CDS             6777..7145
                     /locus_tag="VBD27_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7025403.1"
                     /translation="MAWEFNEKSPIYSQIAEHIKMQIVSQEIKSGDQLPTVRELAQEA
                     GVNPNTMQRAFTELEREGMVFSQRTSGRFVTEDNLLIGKIRQQVAKAELATFVNNMKK
                     IGYKLDEITVALDHFIKEIN"
     gene            7146..7844
                     /locus_tag="VBD27_001398"
     CDS             7146..7844
                     /locus_tag="VBD27_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025404.1"
                     /translation="MTQLLQLHHVTKKYHKHIAVNDVTVSIPTGKIIGLLGPNGSGKT
                     TIIKMINGLLQPDKGDIVIDGYRPSVETKKIISYLPDTSYLQENMKIKDVVTLFEDFY
                     NDFNSKVAYQLFEDLNLNPKERLKNLSKGNKEKVQLILVMSRKARLYILDEPIGGVDP
                     AARDYILKTIISNYSNDASVLISTHLISDIEPILDEVIFLKEGEIDLQGNADDLREEH
                     NCSIDALFRERFKK"
     gene            7856..>8594
                     /locus_tag="VBD27_001399"
     CDS             7856..>8594
                     /locus_tag="VBD27_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HEO7025405.1"
                     /translation="MFGKLLKYELKSVGKWYLTLNAAVLLVSIILGLVLKALGGNFST
                     DTNSTSAQIFTIILVLLLAMVISGSLLSTLAIIIKRFYSNIFGRQGYLTLTLPVTTNQ
                     IICSKLLASLLWSIFNIFIVIIGIILVILPLVGIGQFVVAFPEIYKIISSSNAPLFIA
                     YFFLSYVAGTLLIYLSIAVGQLFTNKRVLMGIVSYFGISLLITFLTLIIDSIFHIDLF
                     NSHANATFSQPVLLYNILVSIVEIAIFY"
BASE COUNT         2789 a         1677 c         1330 g         2798 t
ORIGIN      
        1 gtcaataaaa aaattggtat agtctttttg attttctgtg ataacgagat aaacgctatc
       61 aaaaattcta tctatcttaa aaaaatatat ttcttagcca ttttattaca taaaaagaat
      121 ggtatcaaat aataccattc ttttactact ctgatgctgt aaaaccaaat tcacttagag
      181 ggtatacttt aaaggtaata acaccacgaa tatctttctc tttgattaat ccaaattttc
      241 gactatcatt tttattttgt cgattatcat ttaacacaag ataagaaccc ttaggaactc
      301 tgctttcttt atttcgtgtt aatgtttcaa ctgagaagtc ggaagtaaag ggcatttgac
      361 cgtctttctt ttcagaataa gcatttttca ttttttcaat gtaaggctca tccttgaaaa
      421 catcattcaa atataaaata tcatccatta ctctcacttt ttgatttggc tcaccaataa
      481 cacgactaat atagaaaatc tttccaacct tatagacaat aaagtctttg tacttaggcg
      541 tcctattacg attaacaaca acaacatctc cgttgctaaa ataagaattt gtagcatcct
      601 tatgtacttt aaaggttgca aaaacaaaat atctcaagag aatcaaaatc aatacagcaa
      661 tcaatgctaa aataatattt ctaataaaat cgcgtttaac cataactact cctaatcttt
      721 tttccattat atcatgtttt catagataaa aaaaggaggg taattattaa tatcatatcc
      781 tattctagtc acaataatcg aataataaaa agcaaaacat ataaaaaacc cattatttaa
      841 ggtattctta aataataggt ttgaattcaa aaattattta acagtacgaa cacgcattgt
      901 gttagttcca cctgtaccta caggaacacc tgcaacgata acgatattat cgcctgattc
      961 aacaagtcct gcttcaagtg ctacacgttc tgcaacctca aacatatcat ctgtagatgc
     1021 tggtttgtct gcaaggacag ggataacacc ccagttaatc atcaatgaac gttgtacttt
     1081 ttcatcaaat gtaacagcca aaatgtctgc atctggacgg aatttagaaa tggcacgagc
     1141 tgtattacct gtttcagtaa ttgttacaac aagtttgata tccattgagt gtgttgcatc
     1201 tttaaccgca gatgcaataa catcagtttt gttattacgt gggaatgcag atgagtctaa
     1261 gcgaccatac tcattgagta atgtttgagc atttttatca atagtagcca ttgtacgaac
     1321 tgactcaact gggtatttac cattagctga ctcacctgaa agcattgtag catcagtacc
     1381 atcaataaca gcattgaaga catcagatac ttctgaacga gtcgcacgtg gtttatcagt
     1441 cattgtttca agcatatttg ttgctgtaat aactgcttta ccagctgcat taactttagt
     1501 aatgatcatt ttttggtaaa ctggaaccat ttcaaatgga acttcgatac ccatatcacc
     1561 acgagcaatc ataataccat ctgctgcttc gataatctca tcaatattat cgataccttg
     1621 ttgattttca attttagcaa acaacttaac gtgtccattg ccagtttctt cacaaatagc
     1681 acgaacttca ttaacatctt tagcagtacg tacaaatgag atagcaataa agttaagtcc
     1741 ttgctcaagt ccaaaacgga tatcagcatt atcgcgttct gcaagtgctg ggaaaggaat
     1801 tttagtataa gggatgttta cacctttttg tttaccaata aggccatcat tctcaacaac
     1861 tacttcaaat tcacgagtgt ctttatcttt tgcaaacaca gtaagaccta gtttaccatc
     1921 atcaacaagg atttgcttac caacttcaac gtcatcaaag atgtcaagtc caccagcaac
     1981 attcaatgca atcacttctg gagttgattt gataccttgc ttagtagcaa cacgtaattt
     2041 tgtacctgtt gtatatgaat ggaaatctgc accatcttca aaaagttctg tacgaatttc
     2101 aggtccttta gtatcaagga ggaagccaac tttttgtcct gcaatctctt ctgctttacg
     2161 aacagtagcc atacgagctc cttgctcagc atgatctcca tgtgagaagt tgaaacggaa
     2221 aacgttagca ccttctttaa tcaattgagc aattttttct gctgaagctt ctacgtcaag
     2281 gctttcaccc cagtatccag actcaccaaa cttcttacca ccacggaatt ctaccgcagg
     2341 accaagtgtt gcaacgattt ttacgcgttt attcataata atttatgagc tcctttttat
     2401 ttaaagtgtc aaattgacaa attcttattt actactttgg ttggcaaggt cacgattaag
     2461 tgctgccaag cgaagatccg cttcatgcgg attattaaca acgatttttc cttcatcagt
     2521 caagctgaac aaagcacctt cttctgctaa acctaaaatt ggactttcaa ccatttcttc
     2581 gttgtggaca ccaacggcta aaccaccacg accttctttc aacaattgaa cagcgtacgc
     2641 tcccatacga gatgctaaga cacgatcacg agccgtcgga ctaccaccac ggagcagatg
     2701 tcctaaattc gtcacacgaa gatcgctatc gtctcctgct gctttcattg tttttgcaaa
     2761 ctcatcacca ctcataacac cttctgcaag aacgatgatt tggtgatgtt taccagctgc
     2821 atagccagct ctaacatttg agacaacttc atcaatattg aactcttctt caggaacaat
     2881 aatttgatct gcacctgcag cgatacctga ccaaagagcg atatctcctg catttcttcc
     2941 cataacctca acaacaaaag tacggttatg acttgctgat gtatcacgaa gacggtcaag
     3001 attctcaact gctgtcgcaa ctgctgtgtc aaaaccaata gtatagtcag tgccaacgat
     3061 atcgttatca attgtacccg gcaaaccaac agctgggaaa ccgtgctcag ttagacgcat
     3121 agcaccatga taagaaccat caccaccgat aactactaca ccttcaatac cgtgtttttt
     3181 aagctgttca atccctttaa gctgaccttc aagttcagca aattcaggat aacgggctga
     3241 acgtaaaaac gttcctccac ggttgatagt atccccaaca gaattagcat ccaaagggaa
     3301 aatatcccct gtcaccatac catagtaacc ttggttgatg ccgtaaactt ccataccttc
     3361 agaaattgct ttacgaacaa ctgcacggat agcagcgttc ataccagggg cgtcaccacc
     3421 actagttaaa acagcaatac gtttcattta attggtactc cttttattaa tttaacgatt
     3481 taattatagc atattaattt gtaaatttct gcactttata tgcaatttta ttgataaacc
     3541 gttttcacaa caaattcaga taaatcaagg gctaagccac tatcttttct aaccaaatgt
     3601 actttgcttt ggatagtttc tttagttgtt tcataatgca aatagacagg aatatgccct
     3661 ttatatttgg acaatatttc cgatattttt ttgtcgttat catgattttt tagaagtatc
     3721 caaaaacgtt cctccgttgc ttcttgaaca ccgtttaaaa ccatctgtaa tcgctcatct
     3781 cgcttctgaa tttttccatg caagtaataa tatttccctt cttgtaaatc atctcttaca
     3841 atattgaaag tttcaggaaa aacagtaata tccatttttg aattaatatc cataacagtc
     3901 aagaaagcca tttgttgtcc agaacttttt gtcctaatga ttttgacctg ctttagctga
     3961 actaaaatag tagcttcgct attttcttgg acttgactaa ttggtataaa cggacggcta
     4021 gctttttgtg aaatccccac taaagggtgc ggactgattc ctactcctaa gatatcttgt
     4081 tccatttcat atttctcaga attagagaaa tcttctactt ctaaccaatg atatgaggta
     4141 tctgcaaata aactacctag ttcatttaca aatgtaaata aagaatcaag attagaggtg
     4201 attttccgtc tattgttatc aaattcgtca aatgctccaa tagcaattag cggagaaata
     4261 aaaccacttt tttgaaactg ctgtggcagt ctcattagaa aatcttcaat actctgaaat
     4321 ggttgatttt tagaaatcca ataagccaag tcgttaggaa cagccttaat attttttaaa
     4381 cctaaataaa ttttcttttt atagatgcgg tctctgtatg aaatagtatt aattgaaggt
     4441 ttttcaataa caaaaccaaa atcaatagca tcgattagat aatcactatt tgaataattt
     4501 atcatgatgt cataaaagac atcggaataa tgggctttga aataggctaa ttgaaaagct
     4561 aaagccgaat aagcaaaggc atggctgcga ttaaacccat aacctgcaaa tttagccatg
     4621 cgttcaaata taagtcgagc atcttcttga cgatgtccct tagatactgc accctctaag
     4681 aaggattgag acatcttttg catctcctta gaatttttct tagacatagc acgtctgagc
     4741 aaatcagact tgcctagcgt aaaacctgcg taggtctggg caatctgcat aacctgttct
     4801 tgatagagca ttatgccata ggtaggttgt aaaattggag ctatgacagg atctactaaa
     4861 tcgatttttt ctttaccaaa acgacggttt ataaaattct cagtataatc acttgcacca
     4921 ggcctattca agctggtagt agctaccaca tcttcaaaac actctggcct aatacgtcgc
     4981 aacaaattaa tagctccact ttgctcaaat tgaaaaatcc cctttgtttg accagccgca
     5041 aatagtttta gagtctcttt atcttctaaa tcaatagctt ctaattgtat agagataccg
     5101 tagtccttat cgactttctc cttcattttc tgaacaaatg tcaaatttcg aagacctaga
     5161 aagtccattt ttagaagacc attagcctca acagaactag catcatattg cgttatcatc
     5221 atatcctcac cgttttttaa aggaatatga tctgttaaca aatcgtcact cattactacc
     5281 ccagcggcat gaatagaagt ctgtctagga tttccttcaa tacgcttagc aatatcatag
     5341 gctttttggt attcaatttt actatctatt atttgtctga aagctaagtt gcggttataa
     5401 acactggtca aattatctct gaaatgaatt tttttagtta tgtttgttaa ctcatactct
     5461 gatgcaccaa agcgtttaaa aacatctcga atagcctgtt tcgcaccaaa agttgagaag
     5521 gttacaatct gtgcagaatg cattgaacca tatcgattac gtacatagcg caagaattct
     5581 ccacgataaa tatccggaag atcaatatca atatctggca tcgaataacg ctcttcattt
     5641 aaaaaacgtt caaaaattaa attatgtttg acaggatcga taccggtaat atttaaagca
     5701 tatgagacta aacttcccgc tgctgaacca cgtcccattc ccatatagta gcccttacta
     5761 cgaccaaatc ttaataaatc ccacacaata aggaaatagt catcaaagcc cattttgtga
     5821 atgattgtta attctttttc gagacgctct tgataagcat cgctccataa acctttactt
     5881 tttaagttat tttgagttaa ggttcttaat tcttctccag caggaatttc tctattaaaa
     5941 ataggtaact taaagtcaga atcaaagtga taaacaacat tctctaccaa agattctagg
     6001 ttagctaaag cttcaggaaa acggttcttg aatgctagag tcatttcttg acaaggtttt
     6061 aattcttgat ttttgctcgc atattgtgca tcttttaaca ggacattatc tcttatagca
     6121 tgaagcatct gtaatgtctc aatatcattt ttttgaaaat atcgaacagt atctatggct
     6181 ataataggtt tggtatatgg aatctgcgga gaggtcaaat taacaccaat aacataatca
     6241 aagggtaaat tgagctgatc aagagcatct tgatagggaa gaatcaattg caccccttca
     6301 agatgttctg tcaataaatt tagagacatc ttctcagaca tttgagccgt tgacaacttc
     6361 aagagattct tataaccaac tgtatttatc gcaatacagt aaacttctat tgatctattt
     6421 tcaaccacta tatcaaagct aaatccaact attggtctta ggtttgcctt ttgcgcctct
     6481 tgaataaaat gatatgctgc ataaagatta ctgtggtcca agataccaat agtttgatat
     6541 cctaatgatt tcgatttaga gacatatgtc tttaaatcaa ccaagctatc cataaaagaa
     6601 taaacggttc ttgtatcaag ttgtgcaaac atagtagccc ctttttacga ttttttacta
     6661 ttatactatg aaacaataaa cgattaaagt ctcaatgata attccttgac atttcaaata
     6721 tttttttgta ctatttttat agtacaaaaa gtacaagatt atgaaaggag ttatctatgg
     6781 cttgggaatt caatgaaaaa tcacctattt attcacaaat tgctgaacat ataaagatgc
     6841 aaattgttag tcaagaaata aaaagtggtg accaattgcc taccgtgaga gagttagctc
     6901 aagaagctgg tgttaaccca aataccatgc aacgtgcctt cactgaattg gaacgtgaag
     6961 gaatggtatt ctcacaacgt acttccggac gctttgttac agaagacaac ttactaattg
     7021 gtaaaattcg tcaacaagtt gcaaaagcag agttagcaac ttttgtcaac aacatgaaaa
     7081 aaataggata taaattagat gaaattacgg ttgctctcga tcattttata aaggagatta
     7141 attaaatgac acagttatta caacttcatc atgttacaaa gaaatatcac aaacatattg
     7201 ctgttaatga tgtgactgta agcattccaa caggaaagat tataggttta ttaggtccaa
     7261 atggtagcgg aaaaacaaca atcatcaaaa tgataaatgg attacttcag ccagataagg
     7321 gagatattgt tatcgatggt tacagaccgt ctgttgaaac caaaaaaatt atttcatatc
     7381 tccctgatac ttcatattta caagaaaata tgaaaatcaa agacgtcgtt acattatttg
     7441 aagatttcta taacgatttt aattcaaagg tagcttatca actatttgag gatcttaacc
     7501 taaatcctaa ggaacgtcta aaaaacctat caaaagggaa caaggagaaa gttcaactaa
     7561 tacttgttat gagtcgtaaa gctaggctct acatccttga tgaacctatt ggtggtgtag
     7621 accctgcagc gcgtgactac attttaaaaa caatcattag caactactct aatgatgctt
     7681 ctgttttaat ttcgactcac ttaatttctg acattgaacc tatccttgat gaagttatct
     7741 tccttaaaga aggagaaatt gacctacaag gcaatgctga tgacttaaga gaagagcaca
     7801 actgttcgat tgatgctctc tttcgtgaaa gattcaagaa ataaaggaga ttgatatgtt
     7861 tggaaaatta ttaaaatatg aattaaaatc agttggtaag tggtatctaa cactcaacgc
     7921 tgcggtttta ttagtatcta ttattttagg actggtatta aaggcacttg gcggcaattt
     7981 ttctactgat actaatagta ctagcgccca aatattcact ataatactcg ttttattgtt
     8041 agcgatggtt atttcaggat ctctgttatc cacccttgct attattatca aacgttttta
     8101 tagcaatatc ttcggccgtc aaggatatct aaccttaacg ctcccagtaa ctacgaatca
     8161 gattatttgt tcaaagctac tagcttcgct tctttggagt atttttaata tttttattgt
     8221 cattattgga atcatattgg tcatattacc tttagtaggt attggtcagt tcgttgtcgc
     8281 ttttccagaa atctataaaa ttatttcatc ttcgaacgct ccacttttta tcgcctattt
     8341 ctttctatcg tacgttgcag ggacattact gatttatctc tctatcgctg tagggcaatt
     8401 atttacaaac aaacgtgtat tgatgggaat tgtttcttat tttggaatat cactcttaat
     8461 tactttctta acattaataa tagatagtat ttttcatatt gatttattca actctcatgc
     8521 aaatgcaaca ttctctcaac ctgtattgct ctacaatata cttgttagca ttgttgaaat
     8581 tgctatattc tata
//
LOCUS       DATRQA010000084         8493 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.084, whole genome shotgun sequence.
ACCESSION   DATRQA010000084 DATRQA010000000
VERSION     DATRQA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.084"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            170..1864
                     /locus_tag="VBD27_001400"
     CDS             170..1864
                     /locus_tag="VBD27_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO7025406.1"
                     /translation="MNYKEIYQEWLENDSLGKDIKSDLEAIKGDESEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAIARGIAVSYDVRYQSKEFA
                     ELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMVTASHNPQAYNGYKAYWK
                     EGSQILDDIADQIANHMDAITDYQQIKQIPFEEALASGLASYIDESIEEAYKKEVLGL
                     TINDTNIDKSVRVVYTPLNGVGNLPVREVLRRRGFENIYVVPEQEMPDPDFTTVGYPN
                     PEVPKAFAYSESLGKSVDADVLLATDPDCDRVALEVKDSKGEYIFLNGNKIGALLSYY
                     IFSQRCALGNLPHHPVLVKSIVTGDLSKVIADKYNIETVETLTGFKNICGKANEYDIS
                     KDKTYLFGYEESIGFCYGTFVRDKDAVSASMMVVEMTAYYKERGQTLLDVLQTIYDEF
                     GYYNERQFSLELEGAEGQERISRIMEDFRQNPILQVGEMTLENSIDFKDGYKDFPKQN
                     CLKYYFNEGSWYALRPSGTEPKIKCYLYTIGCTEADSLSKLNAIESACRAKMNSTK"
     gene            2003..2857
                     /locus_tag="VBD27_001401"
     CDS             2003..2857
                     /locus_tag="VBD27_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEO7025407.1"
                     /translation="MTELIDGKALSQKMQAELGRKVERLKEQHGIIPGLAVILVGDNP
                     ASQVYVRNKERSALEAGFKSETLRLSESISQEELIDIIHQYNEDKSIHGILVQLPLPQ
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPMMVPCTPAGIMEMFREYHVDLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRNLSEVTKEADILIVAIGQGHFVTKD
                     FVKEGAVVIDVGMNRDENGKLIGDVVFEQVAEVASMITPVPGGVGPMTITMLLEQTYQ
                     AALRSVSL"
     gene            2854..3690
                     /locus_tag="VBD27_001402"
     CDS             2854..3690
                     /locus_tag="VBD27_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEO7025408.1"
                     /translation="MIVGEQEARALIKPRPKSSHKGDYGSVLLIGGFYPYGGAIIMAA
                     LACVKTGAGLVTVATQSCNIPSLHSQLPEVMAFDSDDYKWLEKSIVQSDVIVIGPGLG
                     VSESSRKILNRTMEKIQSHQSVIIDGSALTLLSEGAFPQTKAKNLVLTPHQKEWERLS
                     GIAVSQQTKENTQTALKSFPKGTILVAKSSHTRIFQDLDEKEIIVGGPYQATGGMGDT
                     LCGMIAGMLAQFKEASPLDKVSVGVYLHSAIAQELSKEAYVVLPTTISDEIPKEMARL
                     SK"
     gene            3816..5156
                     /locus_tag="VBD27_001403"
     CDS             3816..5156
                     /locus_tag="VBD27_001403"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO7025409.1"
                     /translation="MSDYLSVSTLTKYLKLKFDKDPYLERVYLTGQVSNFRRRPNHQY
                     FSLKDDKSVIQATMWSGQFKKLGFELEEGMKVNVVGRVQLYEPSGSYSIIVEKAEPDG
                     IGALAIQFEQLKKKLSQAGYFDDRHKQLLPQFVRKIGVVTSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGEGAAQEIAQTIALANEKKDLDLLIVGRGGGSIEDLWAFNEECVV
                     EAIFESRLPVISSVGHETDTTLADFVADRRAATPTAAAELATTVTKIDILSWITEREN
                     RMYQSSLRLIRTKEERLQKSKQSVIFRQPERLYDGFLQKLDNLNQQLTYSMRDKLQTV
                     RQKQGLLHQKLQGIDLKQRLHTYQERVVQSRRLLSSTMTSQYDSKLARFEKAQDALIS
                     LDSSRIVARGYAIIEKNHTLVSTTNGINEGDHLQVKMQDGLLEVEVKDVRQENI"
     gene            5134..5349
                     /locus_tag="VBD27_001404"
     CDS             5134..5349
                     /locus_tag="VBD27_001404"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO7025410.1"
                     /translation="MSDKKTFEENLQELETIVSRLETGDVALEDAIAEFQKGMLISKE
                     LQKTLKEAEETLVKVMQADGTEVEMDT"
     gene            5349..6221
                     /locus_tag="VBD27_001405"
     CDS             5349..6221
                     /locus_tag="VBD27_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO7025411.1"
                     /translation="MVTIEKIDEAIHRHYKQTHSVVSPDLIKAILYSVDGGGKRIRPR
                     ILLEILEGFGVELIDGHYDVAAALEMIHTGSLIHDDLPAMDNDDFRRGRLTNHKKFDE
                     ATAVLAGDSLFLDPFDLVVKAGFKADVTVRLIELLSMSAGSFGMVGGQMLDMKGENKV
                     LSIDDLSLIHINKTGRLLAYPFVAAGILAEKSEEVKGKLHQAGLLIGHAFQVRDDILD
                     VTASFEELGKTPNKDIVAEKTTYPNLLGLDKSQEILDDTLKKAQAIFQNLEKKANFNA
                     RKIIDIIEGLRLNG"
     gene            6214..7041
                     /locus_tag="VBD27_001406"
     CDS             6214..7041
                     /locus_tag="VBD27_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEO7025412.1"
                     /translation="MAKERVDVLAYKQGLFDTREQAKRGVMAGLVINVINGERYDKPG
                     EKVADDTELKLKGEKLKYVSRGGLKLEKALQVFEISVADKLTIDIGASTGGFTDVMLQ
                     SGARLVYAVDVGTNQLVWKLRQDHRVRSMEQYNFRYAQKEDFKEGLPEFASIDVSFIS
                     LNLILPALKEILVDGGQVVALIKPQFEAGREQIGKNGIVKDKLVHEKVLTTVTNFTKD
                     YGYTVKHLDFSPVQGGHGNIEFLMHLQKCQDPQNLVLDQIQDVIEKAHKEFKKNEEE"
     gene            7028..7501
                     /locus_tag="VBD27_001407"
     CDS             7028..7501
                     /locus_tag="VBD27_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO7025413.1"
                     /translation="MKKSERLNLIKQIVLNHAVETQHELLRRLEAYGVTLTQATISRD
                     MNEIGIIKVPSAKGRYIYGLSNENDPIFTTAVAKPIKTSILSISDKLLGLEQFININV
                     IPGNSQLIKTFIMSHCQEHIFSLTADDNSLLLIAKSEADADHIRQSMIAMLEKKD"
     gene            7513..>8493
                     /locus_tag="VBD27_001408"
     CDS             7513..>8493
                     /locus_tag="VBD27_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7025414.1"
                     /translation="MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNM
                     MLGARASVEVIRHGANKAEIEGFFSIEKNQSLVQLLEENGIELADELIIRREIFQNGR
                     SVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
                     YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDK
                     LMNHKNIADTLTNAYLMLDNEEFSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYY
                     VIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDVNDVLDYF"
BASE COUNT         2836 a         1251 c         1820 g         2586 t
ORIGIN      
        1 ggtgatgaaa aaaatttaga tgctttaaat atattaagtg tagttaattt ataaaataag
       61 ttgactttat aaggttaatt tgatactatt cagttagctc ctgaagaact aaaatctctt
      121 atcatggagc tgatttttac cattttggtc ttagaatagg atgtctcata tgaattataa
      181 agagatttat caagagtggt tagaaaacga ctcactcggt aaagatatta agtcagattt
      241 agaagctatt aaaggagatg aatctgaaat tcaggatcgt ttttacaaaa cattagaatt
      301 tggaacggcg ggattgagag gtaaacttgg agcaggaacc aatcgtatga atacttatat
      361 ggtggggaaa gcagcacaag cattagctaa tacgattatt gatcatggcc ctgaagctat
      421 tgcacgtgga attgcagtta gttatgatgt ccgttatcaa tctaaggaat ttgcagaatt
      481 aacttgttcc attatggcag caaatggtat taagtcttat atttataaag ggattcgccc
      541 aacaccaatg tgctcatatg ctattcgtgc tctaggatgt gtttcgggtg tgatggttac
      601 tgctagtcat aatcctcaag cttataatgg ttataaggca tattggaaag aaggatctca
      661 gattttagat gatattgctg accaaattgc caatcatatg gatgctataa ccgattatca
      721 gcaaattaag caaataccct ttgaagaggc tctggcaagt ggtttggcaa gttatattga
      781 tgagagtatt gaagaagcat ataaaaaaga agtgcttggt ttaactatta atgatactaa
      841 cattgataag tcagtccgag tagtttatac tcctttaaat ggcgtaggaa atttacctgt
      901 gcgcgaagtt ttaagacgcc gtggttttga aaatatttat gtagtacctg agcaggaaat
      961 gcccgatcct gattttacaa cagttggcta tccaaaccct gaagttccta aagcatttgc
     1021 ctattcagaa tctctaggaa agtcagttga tgcagatgtc ttacttgcca cagatccaga
     1081 ttgtgaccga gtagcattgg aagtcaaaga tagtaaggga gaatatattt tcttaaatgg
     1141 taataagata ggggcacttc tttcctatta tattttttca caacgatgtg ccttagggaa
     1201 tttgccacat catcctgtat tggtaaaatc cattgtaact ggtgatctat caaaagttat
     1261 tgcagataaa tataatattg aaactgttga aactttaaca ggatttaaaa atatttgtgg
     1321 aaaagctaat gaatatgata tctcaaagga taaaacttat ctctttggct atgaagaaag
     1381 tattggtttt tgctatggca cttttgtacg tgataaagat gctgtgagtg cttcaatgat
     1441 ggtagtagaa atgactgcct attataaaga acgagggcaa acacttttag acgttttgca
     1501 aaccatttac gatgaatttg gctattacaa cgagcgccaa ttttctcttg agttagaggg
     1561 tgctgagggg caagaacgta ttagtcgtat tatggaggat tttagacaga acccaatatt
     1621 acaagtaggt gagatgacat tggagaattc tattgatttc aaggatggtt ataaggattt
     1681 tccaaagcaa aattgtttaa aatattattt taatgagggt tcatggtatg ctttaaggcc
     1741 gtcagggaca gaacctaaga taaaatgtta cctttatacg attggttgta cagaagcaga
     1801 tagtttatcg aaacttaatg caattgagtc ggcttgtcgt gctaaaatga atagtactaa
     1861 ataagtcata tgtaaaaagg cgatgaggga ttcattgcct tttctattga ttaaaattga
     1921 tacaaaatga atagcgatat aaaatagcat aatcgctatt ttttgatata atatagaaaa
     1981 aacggatgaa gaaggagtat caatgactga attaatagat ggtaaagcct tatcacaaaa
     2041 aatgcaggct gagttagggc gaaaagttga aaggttaaag gaacagcatg gtatcatacc
     2101 tggtttagct gtaatccttg ttggggataa tccagctagc caagtgtacg ttcgaaataa
     2161 agaacgctct gcattagaag ctggttttaa aagtgagact ttacgattat ctgaatccat
     2221 ttctcaagaa gaactgattg atattatcca tcaatacaat gaagataaaa gtatccatgg
     2281 tattttagtt caacttccgt taccacaaca tattaacgat aaaaaaatta ttttagcaat
     2341 agatcctaag aaagatgttg atggtttcca cccaatgaat acgggtcatc tttggtcagg
     2401 acgaccgatg atggtacctt gtacgccagc tggtattatg gagatgttta gagagtacca
     2461 tgtggattta gagggaaaac atgctgttat catcggaagg tccaatattg ttggtaagcc
     2521 aatggcacaa cttctccttg ataaaaacgc aacagttact ttgacacatt caagaactcg
     2581 aaacctttct gaggtaacaa aggaggctga tatccttatt gttgcgattg gtcaggggca
     2641 ctttgttaca aaagacttcg ttaaagaagg tgctgttgtg attgatgttg gtatgaatcg
     2701 cgatgaaaat ggtaaattga ttggagacgt tgtatttgaa caagtggcag aagttgctag
     2761 tatgataaca cctgttcctg ggggcgtagg acctatgacg attacaatgc ttttggaaca
     2821 aacttatcaa gcagctctta gaagtgtgag tctatgattg ttggtgaaca agaagcgagg
     2881 gcgttaatta agccacgccc taaatcaagc cataaaggtg attatggtag tgttcttctg
     2941 ataggaggtt tttatcccta tggaggtgct attataatgg cagctttggc ctgtgtcaaa
     3001 actggtgcag gattagttac tgtagcaacc caaagttgca atatcccctc tttgcatagt
     3061 caactaccag aggtaatggc gtttgatagt gatgattaca aatggttgga aaaatcaatt
     3121 gttcaaagtg atgttattgt aattggtcct ggattaggag tatcagaatc atctcgaaaa
     3181 attttgaacc ggaccatgga gaagattcaa tcacatcaaa gtgtcatcat tgacggatca
     3241 gccttgactc tgttatcaga aggtgcgttt ccgcaaacaa aggctaagaa tttagtgttg
     3301 acacctcatc aaaaagaatg ggagcgattg tcaggtattg ctgtatcgca acagacaaaa
     3361 gaaaataccc aaaccgctct taaatctttt cccaaaggga cgatcttagt cgctaagagt
     3421 tcgcatacgc gtatttttca agatttagac gaaaaagaaa ttatagtagg aggtccttac
     3481 caggcgactg gagggatggg ggatactttg tgtggtatga ttgcaggtat gttagcgcaa
     3541 tttaaagaag cctctcctct agataaggta tcagtgggag tttatctaca ttcggcaatt
     3601 gctcaagaat tatctaaaga agcctacgtt gttctaccga caacgattag tgatgagatt
     3661 ccaaaagaaa tggctagact atctaaataa aaaataagta gagaggtgtt agcatgacac
     3721 ttcttttgct ttatcgagct atttgtagag caaataggta ttttttaagt ttttttggta
     3781 taatgaagtt aataaaactc tcatgaggag gtgctatgtc agactattta tccgtgtcga
     3841 cactgactaa gtatttaaaa ttaaaatttg acaaagaccc ttatttagag agggtttatt
     3901 taactgggca agtgtctaat tttcgtcgtc gcccaaacca tcaatacttc tctttaaaag
     3961 atgataaatc agtcattcag gcgacaatgt ggtcaggtca attcaaaaaa ctgggttttg
     4021 aattagagga agggatgaag gttaatgtag ttggccgtgt tcaactttat gaaccaagcg
     4081 gctcctactc tattattgtt gaaaaagcag aaccggatgg tataggagct cttgctatcc
     4141 aatttgaaca actaaagaag aaactctctc aagcaggtta ctttgatgat cgtcataagc
     4201 aattacttcc tcaatttgtt agaaaaatag gagttgtgac tagtccaagt ggagctgtta
     4261 ttcgggatat tattacaaca gtatctcgtc gattcccagg agtggaaata ttacttttcc
     4321 ccacaaaagt tcaaggagag ggcgcagctc aggagatagc acaaactatt gctttagcta
     4381 acgagaaaaa ggatttggat ctattaattg ttggccgtgg gggaggttct attgaggact
     4441 tatgggcatt taatgaagaa tgtgttgtag aagctatttt tgaatctcgt cttcccgtta
     4501 tttctagtgt cggacatgaa actgatacaa ctttagcaga ttttgtagcc gatcgcagag
     4561 cagcaactcc aactgcagca gctgagcttg ctacaacggt tactaaaatt gatatcttgt
     4621 cttggattac tgaacgtgaa aatagaatgt atcagtctag ccttcgtctt atcaggacaa
     4681 aagaggagag gttgcaaaaa tcaaagcaat cagttatttt taggcaacca gagcgtctat
     4741 atgatggttt ccttcaaaaa ttagataacc taaatcagca gttaacatat tctatgcgtg
     4801 ataaactaca aacagtaaga caaaagcaag gtttgcttca tcaaaaactg caaggcatag
     4861 atttaaaaca gcggcttcat acttaccaag agcgtgttgt gcaaagtaga cgactattat
     4921 caagtacaat gactagtcag tatgacagca agctagcacg ttttgaaaag gctcaagatg
     4981 cgcttatatc attggatagt tctagaattg tagcgcgtgg ttacgctatt atcgaaaaaa
     5041 accatacttt agtatctact actaatggaa taaatgaagg agatcatttg caagttaaga
     5101 tgcaagatgg tcttttagaa gttgaggtga aggatgtcag acaagaaaac atttgaagaa
     5161 aatttgcagg aattagagac aattgtctca cgtttagaaa caggtgatgt tgccttagaa
     5221 gatgccattg ctgaatttca aaaaggaatg cttatttcaa aagagttaca aaaaacttta
     5281 aaagaagctg aagagacgct tgtaaaagtg atgcaagctg acggaacgga agtagaaatg
     5341 gatacttgat ggttactatt gaaaaaattg atgaagctat tcatcgtcat tacaaacaga
     5401 cacatagtgt cgtttctcct gatttaatta aggctatcct ctactcagtt gatggaggtg
     5461 ggaaacgtat tcgtccacgc atccttttag aaattttaga gggatttgga gttgaactaa
     5521 tagatggtca ttatgatgtg gctgctgctt tggagatgat tcacactggc tctttaattc
     5581 atgatgattt accggcgatg gataatgatg attttcgtcg tggccgctta acaaaccata
     5641 aaaagtttga tgaagctaca gcggttttag ctggagattc ccttttttta gacccatttg
     5701 atttagttgt aaaagctggt tttaaagctg atgttactgt taggttaata gagttattgt
     5761 ctatgtctgc gggtagtttt ggcatggttg gtggacaaat gttagatatg aagggagaaa
     5821 acaaagtttt atctattgac gatttaagct tgattcatat taataaaacg ggacgcctgt
     5881 tagcttatcc ctttgttgca gcaggtattt tagctgagaa gtcggaagaa gtaaaaggaa
     5941 aactgcatca agctggcctt ttaatcggtc atgcttttca agtacgtgat gatattttag
     6001 atgtgactgc tagttttgaa gaattgggta agacaccaaa taaagacatt gtagcagaaa
     6061 agacaactta tccaaattta ttgggtttgg ataagtcaca ggaaatactt gacgatactt
     6121 tgaaaaaagc tcaggcaatt tttcaaaatc tagagaaaaa agctaacttt aatgctagaa
     6181 aaataataga tataatagag ggattacggt tgaatggcta aagagagggt agatgttctt
     6241 gcctataaac agggactttt tgatacacga gagcaagcga aacgtggtgt tatggcagga
     6301 ctggtgatta acgttatcaa tggagaacgt tatgataaac caggcgaaaa ggttgcagac
     6361 gatactgaat taaaactaaa aggtgaaaaa ctaaaatatg ttagtagagg tggattgaaa
     6421 ttagaaaaag ctttacaagt ttttgaaatt tcagttgcag ataagctaac tatagatatt
     6481 ggcgcctcta cgggtggttt tactgatgtt atgctacaat caggagcgcg tttagtttac
     6541 gcagtagatg taggaacaaa tcaattagtt tggaagttac gtcaggatca tcgtgttcgt
     6601 tctatggaac aatataattt taggtatgcc caaaaagaag atttcaagga gggactgcct
     6661 gaatttgcat cgatagatgt ctcatttatc tctcttaatt tgattttacc agctctaaaa
     6721 gaaattttag tggatggtgg acaagtagtg gcattaatta aaccacaatt tgaagcaggt
     6781 cgtgagcaaa ttggtaaaaa tggtattgtc aaagacaagt tggttcatga aaaggttttg
     6841 acaacagtga ccaatttcac gaaagattat ggatatacgg ttaaacatct tgatttttcg
     6901 cccgttcaag gtggacatgg aaatattgag tttttaatgc atttgcaaaa gtgtcaagat
     6961 ccacaaaatc ttgtgcttga ccaaatacaa gatgttatag aaaaagcaca taaggaattt
     7021 aagaaaaatg aagaagagtg aacgtttaaa tttaattaag caaattgttc ttaaccatgc
     7081 tgttgaaaca caacatgagt tgttacgcag attggaagct tatggggtaa ctctaactca
     7141 agcaactatt tcacgtgata tgaatgaaat tggcattata aaagtgccat cagcaaaagg
     7201 tcgctatatt tacggtttgt caaatgaaaa cgaccctatc tttacaactg ctgtggcaaa
     7261 gcctattaaa acaagtattt tatcaatatc agataagcta ctaggtttag agcaatttat
     7321 caatattaat gtcataccag gtaacagtca attaattaaa accttcataa tgtcacattg
     7381 tcaagaacat atttttagtt tgacagctga cgataatagt ctccttttga ttgcaaaatc
     7441 agaagcagat gctgatcaca ttcgtcaatc aatgattgca atgttggaga aaaaagatta
     7501 gtagaggacc ttatgctttt agaaatttct attaagaatt tcgctattat cgaagaaatt
     7561 tcacttaatt ttgaaacagg aatgactgtt ttaactggtg agactggtgc agggaaatct
     7621 atcattattg atgctatgaa tatgatgtta ggagcccgtg ctagtgttga agtgattcgc
     7681 catggtgcta acaaagcaga aattgaagga tttttctcta ttgaaaaaaa tcaatcatta
     7741 gtccaattat tggaagaaaa tggcattgaa ttagcagatg aattaattat tcgccgagaa
     7801 atctttcaaa atgggcgcag cgttagtcga attaatgggc aaatggtgaa tttgtcaaca
     7861 ctaaaagctg tggggcatta tttggtagac attcatggtc agcatgacca agaggaacta
     7921 atgaaaccta acatgcacat tctgatgtta gatgaatttg gtaatacaga atttaatgtc
     7981 ataaaagagc gttatcaaag tctttttgat gcttatcgtc agcttcgtaa acgtgtattg
     8041 gataagcaaa aaaatgaaca agagaataaa tcacgtattg aaatgctaga atttcaaata
     8101 gcagaaattg agtctgtagc ccttaaatca gatgaagacc aaacgctact caagcaacgt
     8161 gataaattaa tgaatcataa gaatattgca gatactttga caaatgcata tcttatgtta
     8221 gataacgaag agttttcaag tttatcgaat gttcgttctg caatgaatga ccttatggct
     8281 ttagaagaat ttgatcgaga atataaagat ctttccacca atctttcaga agcttactac
     8341 gttattgaag aagttactaa acgtttaggt gacgttatcg atgatttaga ttttgacgct
     8401 ggtttactac aagaaattga aaatagacta gatgttatta acacaataac gcgaaaatat
     8461 ggcggtgatg tgaatgatgt tttagactat ttt
//
LOCUS       DATRQA010000085         8490 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.085, whole genome shotgun sequence.
ACCESSION   DATRQA010000085 DATRQA010000000
VERSION     DATRQA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.085"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            8..688
                     /locus_tag="VBD27_001409"
     CDS             8..688
                     /locus_tag="VBD27_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO7025415.1"
                     /translation="MDVLIIEDDPMVEFIHRNYLEKLNYFQNIYSTASQTQAIAYLND
                     IKIQLVLLDIHIKEGNGLELLKLLRNQHQNTEVIVISAANEAHTVKEAFHLGIVDYLI
                     KPFTFERFESSIEKFLNHYHTFEADKIYQDNIDHFQKIDSGWLEGEVKLDEKGLSEIT
                     YQHILDAIQELEQPFTIQELAKCSQFSHVSVRKYIAYMEEKGLLTSQQIYTKVGRPYK
                     VYKLIKKT"
     gene            complement(709..1704)
                     /gene="galE"
                     /locus_tag="VBD27_001410"
     CDS             complement(709..1704)
                     /gene="galE"
                     /locus_tag="VBD27_001410"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO7025416.1"
                     /translation="MAVLILGGAGYIGSHMVDQLITQGKEKVIVVDNLVTGHRQAVHS
                     DAIFYEGDLSDKTFMRQVFRENPDVDAVIHFAAFSLVAESMENPLKYFDNNTAGMIKL
                     LEVMNECDIKNIVFSSTAATYGIPEQVPILETAPQNPINPYGESKLMMETIMKWADQA
                     YGIKFVALRYFNVAGDKPDGSIGEDHKPETHLLPIILQVAQGVRDKIMIFGDDYNTPD
                     GTNVRDYVHPFDLADAHILAVDYLRQGNESNVFNLGSSTGFSNLQMLEAARRITGKEI
                     PAQKAARRPGDPDTLIASSEKARQMLGWEPKFDNIDKIISSAWAWHSSHPNGYED"
     gene            complement(1792..3399)
                     /locus_tag="VBD27_001411"
     CDS             complement(1792..3399)
                     /locus_tag="VBD27_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO7025417.1"
                     /translation="MKKHWWHKATIYQIYPRSFMDSDGDGVGDIKGITSKLDYLEKLG
                     ITAIWLSPVYQSPMDDNGYDISDYQAIADIFGDMNDMDQLLQEANQRGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDKASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIDKGIGGFRMDVIDLIGKIPDKGITGNGPKLHDYL
                     KEMNRASFGKHDLLTVGETWGATPDIAKQYSNPDNEELSMVFQFEHVGLQHKPDAPKW
                     DYSDGLDVPALKDIFTKWQTQLELGQGWNSLFWNNHDLPRVLSIWGNDSDNRKQSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFECLADVDDIESLNYAKEAMDNGVSEATILDS
                     IRKVGRDNARTPMQWSQGHQAGFTNGTPWLAVNPNYQEINVEAALNDTESIFYTYQKL
                     VALRKEHDWLVDADFKLLETADKVFAYVRQTDKERYLIVANLSDQNQSFEFPEAVKET
                     IISNTEVQEVLSSNTLKPWDAFCIELF"
     gene            complement(3528..4661)
                     /gene="ugpC"
                     /locus_tag="VBD27_001412"
     CDS             complement(3528..4661)
                     /gene="ugpC"
                     /locus_tag="VBD27_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO7025418.1"
                     /translation="MVELNLNHIYKKYPSASHYSVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIDGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKFSKQEIDKRVREAAANIGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPDGD
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPSMNFFKVKVENGMIISEDGLRIAIPEG
                     QEKLLESRGYKGKELIFGIRPEDISSNLLVQDTYPNANVEAEVLVSELLGSETMLYVK
                     LGQTEFASRVEARDFHNPGEKVNLTFNVAKGHFFDADTEQAIR"
     gene            complement(4762..5613)
                     /locus_tag="VBD27_001413"
     CDS             complement(4762..5613)
                     /locus_tag="VBD27_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7025419.1"
                     /translation="MDFKELFPEVITKQEVKQSEDYIIVEQDGHVLHFPKSSLTKREL
                     CLLQMTQSLEEASSVDSQNPWYRYLVEGRGRLPQSYSAVQFIFIEHQFTLSEELKDFL
                     SPLVINVETIMTINQTQSVMILNQDNFFNATELLTDILPTIENDFNTRLRCYFGNSWT
                     HLQAVDWKELYEEEYKLFTLFLSHKAEQHYCRFPKMALWALANQSPMPSIKAKCLQHI
                     LDTSDTSAIIKALWQEQGNLAKTAKALFIHRNSLQYKLDKFTQSSGLNLKILDDLAYA
                     YLISMDT"
     gene            complement(5708..6604)
                     /locus_tag="VBD27_001414"
     CDS             complement(5708..6604)
                     /locus_tag="VBD27_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEO7025420.1"
                     /translation="MAITIQNHELQVTLKALGATMTSITDSQGVEYLWQGEPTYWGGQ
                     APILFPICGSVRNDCVIYRPAQAPHFTGIIPRHGFVRHKTFDYDYISDSSVRFTIKSS
                     EETLINYPYRFSLEITYTLRNKSIAITYIVKNLESEKNMPYAIGAHPGFNCPLFEKEV
                     FSDYYLEFEQFETCTIPESFPDTGLLDLQARHPFLENQKQLSLNYALFEKDAITLDQL
                     RSKTVYLKSRNHAKGIQLDFDDFENLILWTSNNGGPFLALEPWSSLSTSIEESDILED
                     KQNIVRLNPKQSKQHSIRITIL"
     gene            complement(6658..7635)
                     /gene="lacD"
                     /locus_tag="VBD27_001415"
     CDS             complement(6658..7635)
                     /gene="lacD"
                     /locus_tag="VBD27_001415"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO7025421.1"
                     /translation="MGLTEQKQKHMEQLSDKNGIISALAFDQRGALKRLMAKYQSEEP
                     TVSQIEALKVLVAEELTPYASSMLLDPEYGLPATKVLDDNAGLLLAYEKTGYDTSSTK
                     RLPDCLDIWSAKRIKEEGADAVKFLLYYDVDSSDEVNEEKEAYIERIGSECVAEDIPF
                     FLEILSYDEKITDSSGIEYAKIKPRKVIEAMKVFSNPRFNIDVLKVEVPVNMDYVEGF
                     AQGETAYNKATAAAYFREQDQATLLPYIFLSAGVPAQLFQETLVFAKEAGAKFNGVLC
                     GRATWAGSVKEYVEKGEAGARQWLRTIGFQNIDELNKILQKTATSWKER"
     gene            complement(7637..>8490)
                     /locus_tag="VBD27_001416"
     CDS             complement(7637..>8490)
                     /locus_tag="VBD27_001416"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEO7025422.1"
                     /translation="VTDVSKTPGGKGLNVTRVLSQLGDNVVATGLLGGHFGDFIRSGL
                     DALEIRHQFLSIGGETRHCIAVLHEGQQTEILEKGPHITKDEAGAFLNHLKLIFDAAT
                     IITVSGSLPKGLPSDYYARLISLANHFNKKVVLDCSGEALRSVLKSSAKPTVIKPNLE
                     ELTQLIGRPISYSLDELKSTLQQDIFRGIDWVIVSLGARGAFAKHGNHYYQVTIPKIE
                     VINPVGSGDATVAGIASALEHQLDDINLLKRANVLGMLNAQETLTGHINLTYYQELIS
                     QIQVKEV"
BASE COUNT         2632 a         1713 c         1389 g         2756 t
ORIGIN      
        1 tcttagtatg gacgtattaa ttatcgaaga tgatcctatg gtggaattta tccaccgcaa
       61 ttaccttgaa aaactcaatt attttcaaaa catctattca actgctagtc aaacacaagc
      121 tattgcttat cttaatgaca ttaaaatcca acttgttctt ttagatattc atattaaaga
      181 aggcaatggt ctagaattat taaagttatt acgcaatcaa caccaaaata ccgaagtcat
      241 tgttatttca gcagccaacg aggcgcacac tgtcaaagaa gcatttcatt taggtattgt
      301 agattacctg ataaaaccat ttacatttga acgctttgag tctagtattg aaaaattcct
      361 aaatcattat catacatttg aagctgataa aatctatcaa gacaacatcg atcactttca
      421 aaaaatagat tctggatggc tagagggaga ggttaaactt gatgagaaag ggctctctga
      481 aattacctat cagcatattc ttgatgccat tcaagagtta gagcagcctt tcactatcca
      541 agagttagca aaatgcagtc aattttctca tgtctctgtt aggaaatata ttgcctatat
      601 ggaagaaaaa ggccttctga caagtcagca aatttatact aaggtcggtc gtccttacaa
      661 agtttataag cttataaaaa aaacttaggc ctgtagacct aagttttttt aatcttcgta
      721 gccatttgga tgactagaat gccatgccca tgccgatgaa ataattttat caatattatc
      781 aaatttaggc tcccacccaa gcatttgacg agctttctct gaggaagcaa taagcgtatc
      841 tggatctcct ggacgacgag ctgccttttg agcaggaatt tctttcccag taatacgacg
      901 agccgcttct aacatctgaa ggttagaaaa acctgtcgaa gatccgagat taaacacgtt
      961 tgattcattc ccttggcgaa ggtaatcaac tgctaatata tgagcatctg ccaaatcaaa
     1021 tggatgcaca taatctcgaa cattagttcc atctggagta ttgtaatcat ctccaaaaat
     1081 cattattttg tcacgtactc cttgagcaac ttgaagaatg attggtaaca aatgtgtttc
     1141 tggtttatga tcttccccaa ttgagccatc cggtttatct ccagcaacgt tgaagtaacg
     1201 tagagcgaca aacttaatac cgtaggcttg atcagcccat ttcataattg tttccatcat
     1261 aagcttactt tcgccataag gattaatagg attttgagga gctgtttcta aaataggaac
     1321 ttgttctgga ataccatatg ttgcagctgt tgatgaaaag acaatatttt tgatatcaca
     1381 ttcattcatt acttctaaaa gttttatcat tccagcagtg ttattatcaa aatattttaa
     1441 aggattttcc attgactcag caactagtga aaatgccgca aaatggataa cagcatctac
     1501 atcaggattt tccctaaaaa cttgacgcat aaacgtttta tcggataaat ctccttcata
     1561 aaaaatcgca tctgaatgca ccgcttgacg atgacctgtt accaagttat caacaacaat
     1621 aactttctct tttccttgtg tgattaactg atcaaccata tgcgagccaa tgtaaccggc
     1681 gccacctaaa atcagaactg ccatattttt ctcctatttc aatactcttt taacatcatt
     1741 tgttacctca ttatactgag aataagcttt ttttgcaaat atctaccgaa attagaatag
     1801 ctctatgcag aaggcatccc acggcttgag agtgttactt gatagtactt cttgaacctc
     1861 tgtatttgaa ataatagttt ctttaacagc ttctggaaat tcaaaagatt ggttttgatc
     1921 ggataagttt gcaactatta aataacgttc tttatctgtt tgacgaacat aagcaaatac
     1981 cttatctgct gtttccaaaa gtttaaaatc agcatcaact aaccagtcgt gttctttacg
     2041 taaagcaact aatttttgat aagtataaaa aatactttcc gtatcattta acgctgcctc
     2101 cacattaatt tcctgataat tgggattgac tgctagccat ggggtaccgt tagtaaaacc
     2161 agcttgatgt ccttgactcc actgcattgg agtacgagca ttatcacgac cgactttacg
     2221 aatactatct aaaattgttg cttcagatac cccattatcc atggcttctt tagcatagtt
     2281 aagggattca atatcatcta catctgctaa acactcaaat ggataattgg tcataccaat
     2341 ttcttcacct tgatagatgt aaggcgttcc acgcatcaaa tgtagtagga ttgctagagc
     2401 cttggcagat tgttttctgt tatcactatc atttccccaa atcgagagca cccgaggaag
     2461 gtcgtgatta ttccagaata aagaattcca accttggcca agctccaatt gtgtttgcca
     2521 ttttgtaaaa atatctttca atgctggaac atctaatccg tcactgtaat cccacttagg
     2581 agcgtcaggt ttatgttgaa gacctacatg ttcaaattga aagaccatag ataattcttc
     2641 attatcggga tttgagtatt gttttgcaat gtctggcgtt gctccccaag tttcacccac
     2701 cgtcaataag tcatgctttc caaaactagc tctgttcatt tctttaagat aatcatggag
     2761 ttttggtcca ttaccagtaa tgcccttatc tggaattttc ccaattaaat caatgacatc
     2821 catacggaaa ccaccaatac ccttatcaat ccagaaattc atcatatcat aaattttatg
     2881 acgtaaagct tcattctccc agttgagatc tggctgacgt ttgctaaaaa ggtgcaaata
     2941 atattgacct gatgctttat catattccca agctgagcca ctaaaaatag aagtcaaatc
     3001 gttaggttca tctcgccaaa tataaaaatc acgttcagga ctattagggt tttctcgcgc
     3061 ttctacgaac caagcatgtt catcagaggt atgattcact accaaatcca tgatgatttt
     3121 aatcccgcgt tgatttgcct cttgtaacag ttggtccata tcattcatgt caccgaaaat
     3181 atctgcaatg gcttgataat ctgaaatatc ataaccattg tcatccattg gactttggta
     3241 aacaggtgat aaccagatag cagtaatccc taatttttca aggtagtcta gtttgctagt
     3301 aatcccttta atgtccccca caccatcacc atctgaatcc ataaatgaac gtgggtaaat
     3361 ttgataaatc gtagctttat gccaccagtg tttcttcatt attcttctcc tatcaataat
     3421 tcattcatct tttattttaa cgtttttcct cttttagtgc aagcgtttgc gcaaataaca
     3481 ttataaaaag gctggaaccc aaccttttta tgaatatcgc ttatacctta acgaatagct
     3541 tgttcagtat ctgcatcaaa gaaatgcccc ttagcaacat taaacgttag gttaactttt
     3601 tctcctggat tatggaagtc acgcgcttca acacgagaag caaattctgt ttgacctaac
     3661 ttaacataaa gcatagtttc gctaccgagt aattcagaaa ctaaaacttc tgcttcaaca
     3721 ttggcatttg gataagtatc ttggacaaga agattgcttg aaatgtcttc tggacgaata
     3781 ccaaaaatca attcttttcc tttatagcct cgtgattcta aaagtttttc ttgaccttca
     3841 gggatagcga ttcgaagacc atcttcacta ataatcatac cattttcaac tttaactttg
     3901 aagaagttca tagatggact accgatgaat cccgcaacaa atttattagc aggaagatta
     3961 tagagttctt gtggtgaacc aacttgctca attttaccaa ttgtaccatc accatcaggg
     4021 ttcttagtag ctgacataat gacaatacgg tctgccaaag tcatcgcctc tgtttggtcg
     4081 tgggtaacat aaatagttgt tgaaccaata cgttgatgaa tttttgcaat ttcagcacgc
     4141 attgaaacac gtaatttagc atctaagtta gataaaggct catccattaa gaacactttg
     4201 gcatcacgca caatagcacg tcccatagca acacgttggc gttgtccacc tgacaaatcc
     4261 gcaggtttgc gctctaagaa ttcagtaagg ccaatattag ctgctgcttc acgaacgcgc
     4321 ttatcaattt cttgttttga gaatttacgc aatttgagcc caaaagccat attatcgtaa
     4381 acagtcatat gtggataaag agcatagttt tggaaaacca tagcaatatc acgatcttta
     4441 ggtgatttgt cattgacaac ttcgccatca atcttgagct caccttcact aatatcttca
     4501 agacctgcga tcatacgaag tgttgttgat ttcccacaac ctgatggtcc aacaaaaaca
     4561 ataaattctt tgtctttaat atccaagtca aagtcttcaa cagaataatg cgatgcactt
     4621 ggatattttt tgtaaatatg atttaaattt aattctacca ttagattgac ctcttttatt
     4681 attgtgattt tattatagca aagaaagggt tttcatataa tggtaacctt gcctaaattt
     4741 cttgtgcaaa ctaactaaat tttaggtatc catcgaaatt aaataggcat aggctaaatc
     4801 atctaatatt tttaaattaa gccccgaaga ttgggtaaat ttatctaatt tatattgcaa
     4861 tgaattccga tgaatgaaaa gtgccttagc cgttttagca aggttccctt gctcttgcca
     4921 caaggctttt attatagccg aagtatcact cgtatcaagg atatgttgta aacattttgc
     4981 tttaatagaa ggcatgggcg attgatttgc caaagcccat aaagccatct ttggaaatcg
     5041 acagtaatgt tgttcggcct tgtgactaag aaaaagcgta aaaagtttat attcttcttc
     5101 atataattct ttccagtcca ctgcttgtag atgcgtccaa ctatttccaa aatagcatct
     5161 taaacgcgtg ttaaagtcat tttcaatcgt tggtaaaata tctgttagta attccgtagc
     5221 attaaagaaa ttatcctgat ttaaaatcat gacactttgg gtttgattaa ttgtcataat
     5281 tgtttctacg tttattacta aaggcgacaa gaaatcctta agttcttctg ataaggtaaa
     5341 ttgatgctca ataaaaataa attggactgc cgaataagat tggggcaacc ttccacgacc
     5401 ttcaactaga tagcggtacc aaggattctg agaatccact gaactcgcct cttccaggct
     5461 ttgtgtcatt tgtaataagc atagttcacg cttagttagc gaagattttg gaaagtggag
     5521 gacatgacca tcttgctcaa cgatgatata atcctcactc tgtttaactt cctgtttagt
     5581 aataacttct ggaaacagtt ccttaaaatc catggcatcc tccactacag ttttgttcat
     5641 tatatcatat ttataacctc tttttgagca cagaaaaaga ggagaatgtc tcctcagaaa
     5701 gtttctgtta taaaatagtg atccgaatag aatgctgttt cgactgcttt ggattaagcc
     5761 gtacgatatt ttgtttatct tctaaaatat cagactcttc aatagaagtt gacaaggaac
     5821 tccagggttc caaagccaaa aaaggaccac cattgttact tgtccaaaga ataagattct
     5881 caaaatcatc aaagtcaagt tggattcctt ttgcatgatt tctagatttt aagtagaccg
     5941 tcttagatcg tagttggtct aaagtaatag catctttttc aaataaagca taattaagag
     6001 ataattgttt ttgattttct aaaaaaggat gtctagcttg taaatctaac aagcctgtat
     6061 ccggaaacga ctcaggaatt gtgcaagttt caaactgttc aaactctaga tagtaatcac
     6121 taaatacttc cttttcaaac aaagggcaat taaagcctgg gtgagctccg atagcatatg
     6181 gcatattttt ttctgattct aagtttttga caatatatgt aatggcaatg cttttatttc
     6241 ttaaggtgta agttatttct aaactaaaac gataaggata atttatcaac gtttcttcac
     6301 tacttttaat tgtaaaacga acagaagaat ccgatatgta atcatagtcg aatgttttgt
     6361 gacgtacaaa gccatgcctt ggtatgatac ccgtaaaatg tggagcttga gctggtctgt
     6421 aaatgacgca atcatttcga acacttccgc atataggaaa taaaatagga gcttgcccac
     6481 cccaataagt gggttcccct tgccaaaggt actcaacgcc ttgactatct gtaatagaag
     6541 tcattgttgc acctaacgct tttaatgtca cttgcaactc atgattttgt atcgtaatag
     6601 ccattatttt tcctttcata aagagcaata attgctttac caacttttct ctagcaatta
     6661 tctttccttc caagaagttg ctgttttttg caaaatttta tttagttcat caatattttg
     6721 aaaacctatt gtacgtaacc attgacgtgc accagcttct cctttttcaa catactcttt
     6781 aacacttcca gcccaagttg ctctaccaca aaggacaccg ttaaatttag caccagcttc
     6841 tttagcaaac acaagagtct cttgaaaaag ctgagcagga actcctgcac tcaaaaaaat
     6901 gtaggggagt agtgtcgcct gatcttgttc tctaaaataa gccgcagctg tcgctttatt
     6961 ataagcagtc tctccttgcg caaaaccttc tacatagtcc atattgacag gcacttccac
     7021 cttcaagaca tcaatattaa atcgaggatt tgaaaaaact ttcatagctt caataacctt
     7081 acgtggctta attttggcat actcaatacc tgagctatcc gtaatctttt catcatatga
     7141 caaaatttca aggaaaaaag gaatgtcttc tgcaacacat tcagaaccaa tccgttcaat
     7201 ataagcttct ttttcttcgt ttacttcatc cgaactatca acatcataat aaagtaagaa
     7261 cttaactgcg tctgccccct cttctttaat gcgtttcgca gaccaaatgt ctaaacagtc
     7321 tgggagtcgc ttggtactac tggtatcata acctgttttt tcgtacgcaa gtaaaagacc
     7381 ggcattgtca tctaaaactt tcgtagctgg caacccatat tctggatcta acaacattga
     7441 tgaagcgtaa ggggttaatt cttcagctac caaaacttta agtgcttcta tttgagaaac
     7501 agtgggctcc tcagattgat attttgccat taatcgcttc aatgctccac gttggtcaaa
     7561 agccaaggcc gaaataatac catttttatc actcaattgt tccatatgtt tttgtttttg
     7621 ttccgttaat cccatcctat acctctttca cttggatttg actgattaat tcttgatagt
     7681 aggtcagatt gatatgccct gttaatgttt cttgtgcatt aagcataccc aagacattag
     7741 ctctcttaag taaatttata tcatctagtt gatgttctaa agcagacgcg attcctgcaa
     7801 ccgttgcatc ccctgaacca actggattga taacttctat ttttggaatc gtcacttgat
     7861 agtagtgatt cccatgttta gcaaaagctc ccctagcacc taaggaaaca ataacccaat
     7921 caattcctct aaaaatatct tgctgtaagg tagattttaa ttcgtctaaa ctatagctaa
     7981 ttggccttcc aattaattgt gttaattctt caaggttagg tttaatgacg gtcggcttag
     8041 cactagattt taaaaccgaa cgtaatgctt ctcctgaaca gtctaaaaca actttcttat
     8101 taaagtgatt cgctagggag attaacctag cataataatc tgaggggagt ccctttggta
     8161 aacttcctga tactgttatg atagttgcag catcaaagat aagttttaga tgattcaaaa
     8221 aggcgcctgc ttcatcttta gtaatatgtg gacctttttc tagaatttca gtctgctgac
     8281 cctcatgtaa aactgcaata caatgacgtg tttctccacc aatacttaaa aattggtgtc
     8341 tgatttccaa tgcatctaag ccacttcgga tgaaatctcc aaaatgcccc cctaatagac
     8401 cagttgccac tacattatcg ccaagttgag aaagaacacg agtcacattt aatcccttac
     8461 caccaggtgt tttggagacg tcggtaaccc
//
LOCUS       DATRQA010000086         8442 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.086, whole genome shotgun sequence.
ACCESSION   DATRQA010000086 DATRQA010000000
VERSION     DATRQA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.086"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            44..160
                     /locus_tag="VBD27_001417"
     CDS             44..160
                     /locus_tag="VBD27_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEO7025423.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKHYGKK"
     gene            complement(252..1136)
                     /locus_tag="VBD27_001418"
     CDS             complement(252..1136)
                     /locus_tag="VBD27_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO7025424.1"
                     /translation="MSLIILTVILVLVIVLLITSLYVVKQQTVAIIERFGKYQKTATS
                     GIHIRVPLGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNENNVTDAYY
                     KLIKPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIVKT
                     LITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVGIA
                     QQRKAIVDGLADSIQELKDANVTLTEEQIMSILLTNQYLDTLNTFAINGNQTIFLPNN
                     PEGVEDIRTQVLSALKTR"
     gene            complement(1284..2948)
                     /locus_tag="VBD27_001419"
     CDS             complement(1284..2948)
                     /locus_tag="VBD27_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEO7025425.1"
                     /translation="MSKITTSLFQEMVQAASTRLSKQAEYVNSLNVFPVPDGDTGTNM
                     GMTMENGAKEVSDKPATTVGEVGQILSKGVLMGARGNSGVITSQLFRGFGQSIKDKEE
                     LTGQDLAHAFQNGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     KGAKRALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFKATPATM
                     TEMVNAEHHKAVVGHVATEDIKYGYCTEVMVGLKQGPTYVKEFNYEEFQGYLSNLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVENMRNQHDAQMQKVEVEETVK
                     ETKEYGIIAVVAGDGLAEIFKSQGVDYIISGGQTMNPSTEDIVKAIEKVNARNVIILP
                     NNKNIFMAAQSAADVVDIPAAVVETRTVPQGFTSLLAFDPAKSLETNVADMTNSLSDV
                     ISGSVTLAVRDTTIDGLEIHENDILGMVDGKILVSTPDMEKALKDTFDKMIDEDSEIV
                     TIYVGEDGKQALAETLSEYLEETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            complement(2948..3313)
                     /locus_tag="VBD27_001420"
     CDS             complement(2948..3313)
                     /locus_tag="VBD27_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7025426.1"
                     /translation="MTVKINTRDGQIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQALLRKENYAKGVVVRSSEAGIAVDVYTVMSYGVKISEVSKNIQERVKFNLENQLG
                     LSADMVNVYVQSIKVVGED"
     gene            complement(3445..3633)
                     /locus_tag="VBD27_001421"
     CDS             complement(3445..3633)
                     /locus_tag="VBD27_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO7025427.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKP
                     KKVWVSARALKSGKVERV"
     gene            3872..4789
                     /locus_tag="VBD27_001422"
     CDS             3872..4789
                     /locus_tag="VBD27_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO7025428.1"
                     /translation="MTRKIGIIGMGHVGSTVAHALIAQGIGDDYVFIDTNVDKVNADM
                     IDFQDALANATYHANIVLNDYDALSDADVVISALGDISLQNNKEANRFAELPFTSKEV
                     YDVSQKLKSSGFRGILIVVTNPVDAITQLYQEYTQFPKSRVIGTGTLLDTARMKRVLG
                     QRLQVDPRSIGGYNLGEHGNSQFVAWSQVTVKGQPAISLLKVEELEQIKEASLKGGHT
                     VFFGKKYTSYGIATAAVRIVLAVLNDSHELLPVSTFYESEGVYLGYPAIIGRVGIVER
                     ISLSLSPKEEQQLTDSAAFIRNYLKHINL"
     gene            complement(4874..5755)
                     /locus_tag="VBD27_001423"
     CDS             complement(4874..5755)
                     /locus_tag="VBD27_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEO7025429.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKLCKQLIETLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLEKAREVVAKAHAKGISVEAEVGTIGGEEDGIVGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQI
                     QEAIKLGVAKVNVNTECQLAFCQATRDYAVEFNANEAEYMKKKLFDPRKFLKPGFDAI
                     TEAVEERIDVFGSANKA"
     gene            complement(5895..7283)
                     /gene="argH"
                     /locus_tag="VBD27_001424"
     CDS             complement(5895..7283)
                     /gene="argH"
                     /locus_tag="VBD27_001424"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEO7025430.1"
                     /translation="MTKNHKLWGGRFESSLEKWVEEFGASISFDQKLAPYDMKASMAH
                     VTMLGKTDIISQEEAGLIKDGLKILQDKYRAGQLTFSISNEDIHMNIESLLTAEIGEV
                     AGKLHTARSRNDQVATDMHLYLKDKLQEMMKKLLHLRTTLVNLAENHIYTVMPGYTHL
                     QHAQPISFGHHLMAYYNMFTRDTERLEFNMKHTNLSPLGAAALAGTTFPIDRHMTTRL
                     LDFEKPYSNSLDAVSDRDFIIEFLSNASILMMHLSRFCEEIINWCSYEYQFITLSDTF
                     STGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKSLPLAYNKDLQEDKEGMFDS
                     VETVSIAIEIMANMLEKMTVNEHIMMTSTETDFSNATELADYLASKGVPFRKAHEIVG
                     KLVLECSKNGSYLQDIPLKYYQEISELIENDIYEILTAKTAVKRRNSLGGTGFDQVKE
                     QILLARKELKAE"
     gene            complement(7302..>8442)
                     /locus_tag="VBD27_001425"
     CDS             complement(7302..>8442)
                     /locus_tag="VBD27_001425"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEO7025431.1"
                     /translation="VAIAWLKKDYDVIAVCMDVGEGKDLDFIHDKALTIGAIESYILD
                     VKDEFAEHFVLPALQAHAMYEQKYPLVSALSRPIIAQKLVEMAHQTGATTIAHGCTGK
                     GNDQVRFEVAIAALDPELKVIAPVREWQWHREEEITFAKANGVPIPADLDNPYSIDQN
                     LWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDITFQNGKPIAINNQEMTL
                     ADLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAMVLLAAHKEIEDLTLVRE
                     VSHFKPILENELSNLIYNALWFSPATKAIIAYVKETQKVVNGTTKVKLYKGSAKVVAR
                     HSSNSLYDENLATYTAADNFDQDAAVGFIKLWGLPTQVNAQVNKG"
BASE COUNT         2526 a         1709 c         1364 g         2843 t
ORIGIN      
        1 ctggtgtctg ttaacgttaa taaattgtct ataggaggta atcatggctg aaaaaacaga
       61 cctatcgtca gcctatcgca gacttaaaag tcctaacatt aaaactagaa aacgtgctct
      121 gaaaatcatt catgagtaca aacattacgg taaaaaataa cttttttccg tctctccaat
      181 tataataaat aagtcagaca caaaataacc agagcaaatg ctctggttat tttgcttcta
      241 taaaaagttc attatcttgt tttaagagca gataaaactt gtgtacgaat atcttcaaca
      301 ccttctgggt tatttggtaa gaaaatcgtt tgattaccat taatagcaaa tgtatttaaa
      361 gtatctaaat attgatttgt taacaagata gacatgattt gttcttctgt caatgtcacg
      421 ttagcatctt tcaattcttg aatcgaatca gctaacccat ctacaattgc tttacgttgt
      481 tgcgcaatac ccacaccatg tagacgatct ttttctgctt ctgcttctgc agcagttaca
      541 attttgatct tatcagcatt cgctaattct tgagctgcta cacgtttacg ttgtgcagca
      601 ttgatttcgt tcatggattg ttttacttct gcatctggct caaccttagt aattaaagtc
      661 ttaacaatga tgtagccgta tgttgacatt tcttctgcca cttgatgttg aacttccaag
      721 gctatttcat ctttcttttc gaatagctca tctaatgtaa gtttaggaac tgatgaacgc
      781 aaagcatctt cgatataaga tttaatctga gcttctggct ttattaattt gtaataagcg
      841 tctgtaacgt tattttcatt gacacgatat tgtgtagcaa tatttaaagt gacaaaaaca
      901 ttatcttttg tttttgtctc aactataatt tcactctgta aaagacgtaa ctgcactcgt
      961 gctgcaatct tatcgatacc caatggtaca cgaatatgaa taccgctagt agcagtcttt
     1021 tgatatttac caaagcgttc aatgatggct accgtctgtt gtttaacaac gtataaagaa
     1081 gttatcaaca gaacaatcac taaaaccaaa ataaccgtaa gaataattaa tgacatcata
     1141 aatctccttt gtatgaatgt tttaaaacaa ttctagcaca atttgtcaaa attttcaaaa
     1201 tactgatgct ttatcatact aaaatagaaa ctctgataaa ataaaaaatt agaagtatct
     1261 ctcctaattt tttattttat caattattca acgctcatga ggtatggata tacaggttga
     1321 tctccttggt ggatctcaac ttcaacatct tcataagtct cctcaagata ttccgacaag
     1381 gtctcggcta atgcttgttt accatcttca ccaacataga ttgtcacgat ttcactatct
     1441 tcatcaatca ttttatcaaa agtgtcttta agtgcttttt ccatatctgg tgtagatact
     1501 agaattttac catcaaccat cccaaggatg tcgttttcat gaatttcaag gccatctatc
     1561 gttgtatcac gaacagctaa agtcacacta ccactaatga catctgagag gctatttgtc
     1621 atatcagcta cattagtttc aagagactta gcaggatcaa aagcaagtaa gctagtaaat
     1681 ccttgtggaa ctgtacgtgt ctctactact gctgctggaa tatcaacgac atctgcagct
     1741 gactgtgcag ccatgaagat gttcttattg tttggtaaaa taataacatt tctagcgttt
     1801 actttttcaa tcgcttttac aatatcttct gtagatgggt tcatagtctg gccgccagag
     1861 atgatataat caacaccttg tgatttaaaa atctcagcta gaccgtcacc agctacaaca
     1921 gcaataatac catattcttt agtttctttg acagtttctt caacttcaac cttttgcatt
     1981 tgggcatcat gttggttacg catgttttca actttaacct tcacaagcga tccatatttc
     2041 aaaccttctt gcataacaag acctggatct tctgtatgaa catgaacctt gacaatttca
     2101 tcatcattaa caactagtaa agaatcccct agattgctaa gataaccttg aaattcttcg
     2161 tagttaaatt ccttaacgta agttggtcct tgctttaaac caaccatgac ttctgtacaa
     2221 tagccatatt taatatcttc tgtggctacg tggcctacta cagctttatg gtgctctgca
     2281 ttaaccattt cagtcattgt tgcgggagtt gctttaaaat cttctgatgc gatatattct
     2341 ccagtcagtg ctgagaggaa accttcgtaa ataaagacga gtccttgacc accagaatca
     2401 acaacaccaa cctctttaag aacaggtagc atatccggtg ttttagcaag agcacgctta
     2461 gcacctttga gagttgcgcg cataacttca actgcatcat cagtttcttc agcttttttt
     2521 agagcagcgg tagctgcacc gcgtgatact gtcaagatag ttccttcaac tggtttcata
     2581 acggctttat aagctacttc aacaccattc tggaaagcgt gtgccaaatc ttgacccgtt
     2641 aattcttctt tatccttgat actttgacca aaaccacgga ataattgtga tgtaattaca
     2701 cctgaatttc cacgtgctcc catcaataca cctttagaaa gaatttgacc aacttctcca
     2761 acagtcgtcg ctggtttgtc cgaaacttcc ttggctccgt tttccatagt catccccata
     2821 ttcgtaccag tatcaccatc tggaactggg aatacattta atgagttgac atactctgcc
     2881 tgtttactca gacgtgtact ggcagcttgt accatttctt ggaataaact tgtcgtaatc
     2941 tttgacacta atcttctcct acaaccttaa tactttgcac ataaacattc accatatcag
     3001 cagataaacc aagctgattt tcgagattga atttcactcg ctcttgaata ttttttgaaa
     3061 cttcactaat tttgacgccg taactcatga cagtataaac atctactgcg atgcccgctt
     3121 cactagacct cacaacaaca ccttttgcgt agttttcctt acgcaaaagt gcttgaaagt
     3181 tatctttgat agcactttta cttgccatac caacaacgcc aaaaatttcc gttgctgaac
     3241 ctccaacgac agttgcaata acgtcatcag agagttcaat ttgaccatct cttgtattga
     3301 tttttacagt catatctact acctcaaaaa gtttttatca ctctattcta tcatatctca
     3361 ctattattgt aaaaaaatag aggtatctaa ctatcaaatc agccttagaa acactaaaaa
     3421 aagccctaag gctttctcag tttattaaac gcgttctact ttaccagatt taagcgcacg
     3481 agctgaaacc caaacttttt ttggtttacc gtcgataaga acagtaactt tttgaaggtt
     3541 tggtttaaca gtacgttttg tttggttcat cgcgtgtgaa cggttgttac cagaaactgt
     3601 tttacgtcct gtgaaataac atactttagc cattttacga gttcctccta tattgatcaa
     3661 tatttacgga tgtgctagca ccacatacta gactattcta tcacaactta cttcttctag
     3721 caagttcttt tcaaaattac tttttcattt gttttatcta aatcgcttac atatttttta
     3781 taataagaca cacttccttc taattcattt tttagtttac attttctttc aaaatattat
     3841 aatagacaac aataatcaaa ggaggtttca tatgacacgt aaaattggaa ttataggcat
     3901 ggggcacgtt ggatcaactg ttgcacacgc tcttattgca caaggtattg gtgatgatta
     3961 tgtctttatt gatactaatg ttgataaggt taatgctgat atgattgatt ttcaagatgc
     4021 tctggcaaat gctacttatc acgctaatat agtattaaac gactatgacg cgttgtcaga
     4081 tgctgatgtt gtgatttcag ccttaggtga tatttcacta cagaataaca aagaagctaa
     4141 tcgctttgct gaactaccat ttaccagtaa agaagtctac gacgttagcc aaaagttaaa
     4201 atcatctggt tttagaggga tccttattgt cgttactaat cctgttgatg ccatcaccca
     4261 gctttatcaa gaatatactc aatttcctaa atcacgcgtt atcggaacag gaaccttatt
     4321 ggatactgct cggatgaaaa gagtgcttgg tcaacgccta caagttgatc caaggagtat
     4381 cggtggttat aatctaggag agcatggtaa ctcccaattt gtcgcttgga gtcaggtaac
     4441 tgtaaaagga caaccagcta tatcactatt aaaagtagaa gaattagaac agataaaaga
     4501 agcttcttta aaaggcgggc acactgtctt ttttggtaaa aaatatacta gttatggtat
     4561 cgctaccgca gctgtccgca tcgttttagc tgtgttaaat gattcccatg agttactacc
     4621 tgtatctacc ttctatgagt ccgaaggggt ttaccttgga tatccagcta ttattggtag
     4681 agtaggaatt gtagaaagaa tttctctttc actaagccca aaagaagagc aacaactcac
     4741 agattcagcg gcatttattc gaaattatct taagcatatc aatctttaaa gagtatctat
     4801 ttaataaaaa gtcatataat ccaaaaaaag ctcaccataa gtgagctttt aactattatc
     4861 tgttttatgt taattatgct ttgtttgctg aaccaaacac gtcaatacgt tcttcaactg
     4921 cttctgtaat tgcatcgaaa ccaggtttca agaatttacg tgggtcaaag agttttttct
     4981 tcatgtattc agcttcgtta gcgttgaatt caacagcgta gtcacgagtt gcttgacaga
     5041 aagctaattg acattctgtg ttaacgttaa ctttagcaac accaagtttg atagcttctt
     5101 ggatttgatc atcaggaata cctgaaccac cgtgtaacac gattgggaaa ccaggaacag
     5161 cttcagtcaa tttcttcaag tgatcaaggt caagtccttc ccagtttgct gggtatggtc
     5221 catgaatgtt accgatacct gcagccaaga agtcaatacc agtttcaacc atagctttag
     5281 cgtcttcgat tggtgctaat tcacctttac cgacaatacc atcttcttca ccaccgatag
     5341 taccaacttc agcttctact gaaatacctt tagcatgtgc tttagcaaca acttcgcgtg
     5401 ctttttcaag gttttcttca actggaaggt gtgaaccatc aaacataatt gaagtgtaac
     5461 ctacttcaat acactcaagg gcatcatcgt aatgaccgtg gtcaaggtgg atagcaactg
     5521 gtacagtaat acccattgat tcaaccaaag tttcaataag ttgtttacaa agtttgtaac
     5581 cacccatgta tttagccgca cccattgaag tttggataag tactggagct tttttagctt
     5641 ctgctgcgcg caagatagct tgagtccact caaggttgtt tgtgttaaat ccacctactg
     5701 catagccgtt atcacgagct gcttggacga atttttctgc tgaaacgatt gccattattc
     5761 aggcctccta tttatttttt gggcgttgcc cacttacatg ttaattctag cacaattcta
     5821 taaaaaatgc tagtttttta ctgaaaatac gtgagaaact taacagagtt aaacgaggtt
     5881 ttttcagaaa gcagtcattc cgctttcaat tctttacgtg ccaacaggat ctgttcttta
     5941 acctggtcaa accctgttcc tcctaatgaa tttcttcgtt taactgctgt ctttgcagtt
     6001 aatatctcat agatatcgtt ttctatcaac tctgaaattt cttgataata ttttaaaggt
     6061 atatcctgta aataagaacc atttttactg cattctaaaa ccaatttacc tacaatttca
     6121 tgtgcttttc tgaaaggtac ccccttactt gctaaataat ctgctaattc tgttgcattt
     6181 gaaaaatcag tctccgttga ggtcatcatt atgtgctcat tcacagtcat tttctcaagc
     6241 atattagcca tgatttcaat agctatgctg actgtttcta cgctatcaaa catcccttct
     6301 ttatcttctt gtaaatcttt gttataagct aacggcaatg atttcataac agttagtaaa
     6361 ctaaataaat ttccataaac acggcctgtc ttccctcgaa ttaattctgc catatcagga
     6421 tttttctttt gaggcataat agatgatcct gtagagaaag tatctgataa tgtgatgaat
     6481 tgatattcat aactgcacca attaatgatt tcttcacaaa aacgtgataa atgcatcatt
     6541 aaaatgctag cattggataa aaattctatg ataaagtcac gatctgagac agcatcaagc
     6601 gaattactat atggtttttc aaaatctaat aaacgagttg tcatatggcg gtcaatagga
     6661 aatgttgtcc ctgctagagc tgctgctcct aatggtgata gattggtgtg tttcatatta
     6721 aactctaatc gctctgtatc acgcgtaaac atattatagt aagccattag atgatgtcca
     6781 aatgaaatcg gctgagcatg ttgtaaatgt gtatatcctg gcataacagt atagatatga
     6841 ttttctgcta aattaactaa ggttgttcgt aaatgaagca atttcttcat catttcttgc
     6901 aatttatctt tcaaataaag gtgcatatct gtagccactt gatcatttcg tgaacgtgca
     6961 gtatgaagct tacctgctac ttctccaatt tcagcggtta ataagctctc tatgttcata
     7021 tgaatgtctt catttgatat gctaaaagtc aattgacctg ctctgtattt atcttgcagt
     7081 atttttaaac catcttttat caagcctgct tcttcttgac taataatatc cgttttgcct
     7141 aacattgtga catgggccat agaagccttc atatcatagg gagctaactt ttggtcgaaa
     7201 ctgattgatg caccgaactc ttcaacccat ttttcaagac tactttcaaa acgaccaccc
     7261 cataacttat gattttttgt catcctctta tcttccttta tttatccttt gtttacttgt
     7321 gcattaactt gggttggaag tccccagagt ttgataaaac caactgctgc atcttgatca
     7381 aagttatcag cagctgtata agttgctaag ttctcatcat ataatgaatt agacgagtgg
     7441 cgagcaacga ctttggcaga acctttatat aacttaactt ttgtagtacc attaaccact
     7501 ttttgtgttt ctttgacata ggcaatgata gcttttgtag ctggactaaa ccataaagca
     7561 ttataaataa gatttgatag ttcattttca aggataggtt taaaatgaga cacttcacgg
     7621 actaatgtta aatcttcaat ttctttatga gcagctaaca gtaccattgc cgcaggacat
     7681 tcataaattt cacgtgattt gataccgact aaacgatttt caacatgatc aatgcgtcca
     7741 ataccatgtt ttcctgcaat ttcatttagc gataaaatca aatctgctag tgtcatttct
     7801 tgattgttta tagctatagg cttcccattt tggaacgtaa tatcaatata ttctgcacaa
     7861 tcaggagctt cttctggtga ttttgtaata ccaaaagctt cttctggcgc ttgattccaa
     7921 ggattctcaa gcacaccaca ttcattggca cgtccccata aattttggtc tatagaataa
     7981 gggttatcta aatctgctgg aataggtacg ccgttagctt ttgcaaaagt aatttcctct
     8041 tcacgatgcc actgccactc acgaacaggg gcaattactt ttaattcagg atctagtgct
     8101 gcaattgcaa cttcaaaacg cacttggtca tttcctttac ctgtacatcc atgtgcaatt
     8161 gtggtcgcac ctgtttgatg tgccatttca actaattttt gggcaataat tggacgacta
     8221 agcgctgaaa caagcgggta tttttgttca tacatcgcat gtgcctgtaa agctggtaaa
     8281 acaaaatgct cagcgaactc atctttaaca tctaaaatat aagattctat ggcacctatt
     8341 gttaaagctt tatcgtgaat aaaatcaaga tctttacctt cgccaacatc catacagaca
     8401 gcaataacgt cataatcttt ttttaaccaa gctatagcaa ca
//
LOCUS       DATRQA010000087         7986 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.087, whole genome shotgun sequence.
ACCESSION   DATRQA010000087 DATRQA010000000
VERSION     DATRQA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7986
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.087"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            229..561
                     /gene="gpsB"
                     /locus_tag="VBD27_001426"
     CDS             229..561
                     /gene="gpsB"
                     /locus_tag="VBD27_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEO7025432.1"
                     /translation="MASIIYSPKDIFEQDFKVSMRGYDKKEVDVFLDDVIKDYENYLE
                     QIEKLQMENRRLQQALDKKESEASNVRNSGTAMYNQKPIAQSATNFDILKRISRLEKE
                     VFGRQIRE"
     gene            580..948
                     /gene="rnpB"
                     /locus_tag="VBD27_001427"
     ncRNA           580..948
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VBD27_001427"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            1031..2185
                     /locus_tag="VBD27_001428"
     CDS             1031..2185
                     /locus_tag="VBD27_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEO7025433.1"
                     /translation="MKESFKLIATAAAGLEAIVGREIRNLGIDCQVENGRVRFHGDIK
                     TIIETNLWLRAADRIKIIVGEFPARTFEELFQGVYGLDWENYLPLGAKFPIAKAKCVK
                     SKLHNEPSVQAISKKAVAKKLQKVFHRPEGVPLQENGAEFKIEVSILKDKATVMIDTT
                     GSSLFKRGYRAEKGGAPIKENMAAAIIQLSNWFPDKPLIDPTCGSGTFCIEAAMIGMN
                     IAPGFNRDFAFEAWPWVDQSQVQKVRDEAESKANYDIDLDISGFDLDGRMVEIARKNA
                     EEAGLGDVIKLKQMRLQDLKTDKINGVIISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDEGFEKKYGSQADKKRKLYNGTLKVDLYQYYGERVRRQVK"
     gene            2198..3685
                     /locus_tag="VBD27_001429"
     CDS             2198..3685
                     /locus_tag="VBD27_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025434.1"
                     /translation="MSEDQKHPFFEPEKETDGLEFKDAKEMTVEEAVRKDSEIKAGIT
                     EEDSILDKYIKQHRDEVASQKFETKSSDFANLDTASLDDFIKKQREELSAMLAAEELS
                     KKLDNSVSQEQDIEANAVSPKEESSQEQENSVTPVPPLNTEAEPTATEPDSTIADSEE
                     YKSSSKKRGGIVGTLIALILLLIVTIFGYNYFKNNNSTNSQTATSQSSSSKATTTSSE
                     EDKKASQNLDNFNKAYANFFVDDKKTQLKNSEFDKLSELEKKVDALKGTKYYGKVKVK
                     FDSLKRQIDAVKAVNDKFKSPAVVDGKKSEKLEVKDGANFDSLDSKTLNTGNASLDSL
                     LHSIVSTGRNQVKQSEEQAKKTQEEQSSSNKVSDTQTTEQPNVTNGQSSSSAATINNQ
                     AAGTASGNLERNRSRVPYNNAAIADTGNPAWIFNPGVLEKIVATSQARGYFSGNNYIL
                     EPVNIINGNGYYNMFKLDGTYLFSINAKTGYFVGNAPGRADSLDY"
     gene            complement(3910..4392)
                     /locus_tag="VBD27_001430"
     CDS             complement(3910..4392)
                     /locus_tag="VBD27_001430"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEO7025435.1"
                     /translation="MTKEVVVESFELDHTIVKAPYVRLISEEVGPVGDIITNFDIRLI
                     QPNENAIDTAGLHTIEHLLAKLIRQQINGLIDCSPFGCRTGFHMIMWGKQDATEIAKV
                     IKSSLEAIAGGVTWEDVPGTTIESCGNYKDHSLHSAQEWAKLILSQGISDNAFERHIV
                     "
     gene            4581..6188
                     /locus_tag="VBD27_001431"
     CDS             4581..6188
                     /locus_tag="VBD27_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEO7025436.1"
                     /translation="MFNIILAMVCALIGLIIGYVAISMKMKSSKEAAELTLLNAEQDA
                     VDLRGKAEIEAEHIRKAAERESKAHQKELLLEAKEEARKYREEIEKEFKSDRQELKQM
                     EARLTDRASSLDRKDENLSNKEKMLDSKEQSLTDKSRHINEREQEIATLETKKVEELS
                     RIAELSQEEAKDIILADTEKDLAHDIATRIKEAEREVKDRSNKIAKDLLAQAMQRLAG
                     EYVTEQTITTVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVVLSGFDPI
                     RREIARMTLESLIQDGRIHPARIEELVEKNRLEMDQRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSYGQNVLRHSVEVGKLAGILAGELGENVDLARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPIVVNTIASHHGDVEPDSVIAVIVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIANGFEGVQNAFALQAGREIRIMVQPGKVSDDQVVIMSHKVRE
                     KIEQNLDYPGNIKVTVIREMRAVDFAK"
     gene            6317..7213
                     /locus_tag="VBD27_001432"
     CDS             6317..7213
                     /locus_tag="VBD27_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025437.1"
                     /translation="MAKDIIKVDHIFKSIGQKTILEDISFSIASNQCVALIGPNGAGK
                     TTLMSTLLGDISISSGALTIFNLPAHHNRLKYKVAILPQENVLPSKFTVRELIDFQRC
                     LFPEVLPMSLILDYLQWSDAHLQQFTETLSGGQKRLLAFVLTLVGKPQLLFLDEPTSG
                     MDTSTRQRFWELVATLKKEGDTIVYSSHYLEEVEHTADRILVLHKGKLLRDTTPFAMK
                     QEKTEKLFTVPLSYQKLLPTYLITECEAKSDSITFVTGEAETVWKILADNGCPIEAIE
                     MTNRTLLNRIFETTKEVKHENL"
     gene            7200..7940
                     /locus_tag="VBD27_001433"
     CDS             7200..7940
                     /locus_tag="VBD27_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025438.1"
                     /translation="MRIFKALLKMEFLLTKRQLANLIMAIGMPVAFFLFFSGFMGEGL
                     TKAIEAIYVRNYMITMAGFSSLSFAFFTFPFSMKDDQLSNRMQLLRHSPVPMWQYYLA
                     KIIRILFYYCLAITVVFLTGHILRQVSMPIEQWMQSFLLLLGGATCFIPFGLLVSYFK
                     NTELMSMVANICYMSLAVLGGMWMPITMFPKWLQGLSKLTPTYHLTQVILSPFANSFA
                     VFSLIILIGYGIIMLVIAYLLSQKRHSI"
BASE COUNT         2730 a         1276 c         1589 g         2391 t
ORIGIN      
        1 gaaaatgaaa ctaatttaaa atttttgcta gaaatgatgg aaaaaaagga agcttatgat
       61 atttctttcc taacttttga tcgcttaaat gaaatatatg aggaatgatc gttttttcgg
      121 atatgagaac gttttacgct tgatttttcc atttaattta ggtattattt actttgatat
      181 aaaggatagg acttatatta aaacgatttg actggtttgg agaatagaat ggcaagtatt
      241 atttatagcc caaaagatat ttttgagcaa gattttaaag ttagtatgcg gggatatgac
      301 aagaaagaag tcgatgtatt tctggatgat gtcatcaaag attacgaaaa ttatcttgag
      361 caaattgaga agctccaaat ggaaaataga cgtctacaac aagctttaga caaaaaagaa
      421 agcgaagctt caaatgtaag aaattcaggg acagctatgt ataatcaaaa accgattgcc
      481 caatctgcaa ccaattttga tattttaaaa cgcattagcc gccttgagaa agaagttttt
      541 ggaagacaaa ttagagaata aggatttctc aaatatgcaa tttttggata atcgcgtagt
      601 attgatatac tatgaggaaa gtccatgcta gcactggctg tgatgccagt agtgtttgtg
      661 ctaggcgaaa aaataagcct agggagatag ctagctatct tacggcaagc aaaagggcta
      721 agtctttgga tatgcctgaa tagctttgaa agtgccacag tgacgtagtt tctagggaaa
      781 tctagaaagt ggaacgcggt aaacccctca agctagcaac ccaaactttg gtaggggcat
      841 gggatagttg gaatgagaac aatctatcct gactgctttg cagtagacag atgattatcg
      901 aaagaaacaa ttcctagttg tttctggaac aaaacatggc ttatagaaaa ttgcataata
      961 ggttagtggc tgagataaca tctcagccaa tttttaactg tgatgaatac gatagtgtaa
     1021 aagaggaaaa atgaaagaat cattcaaatt aatagcaact gctgctgctg gtttagaagc
     1081 cattgttggt agagaaatca gaaacctagg aattgactgc caagttgaga acggtagagt
     1141 ccgtttccac ggagacatca aaacaatcat tgaaacgaac ctatggctac gagctgccga
     1201 tcgtataaag attattgtag gagagtttcc agcacgtact tttgaggaac tttttcaggg
     1261 tgtatatggc cttgactggg aaaattattt accgttaggg gcaaaatttc ctattgctaa
     1321 agcaaagtgt gtcaagtcaa aattgcacaa tgaacctagt gtacaagcca tttctaaaaa
     1381 agctgttgct aagaaacttc aaaaagtctt tcaccgtcct gaaggagttc ccttacaaga
     1441 aaatggagct gaatttaaaa tagaagtttc tattttaaaa gataaagcga ctgtcatgat
     1501 tgatacgact ggatcaagtc tcttcaaacg tggttaccga gcagaaaaag gtggtgcgcc
     1561 aattaaagaa aacatggcag cagctattat tcaattatct aattggttcc ctgataagcc
     1621 attgattgat ccaacttgtg gttcaggtac tttttgtatt gaagccgcta tgattggaat
     1681 gaatatagct cctggtttta atcgtgattt tgcctttgag gcgtggccat gggtagatca
     1741 aagtcaagta caaaaggttc gcgatgaagc tgagagtaaa gcaaactatg atattgattt
     1801 agatatttca ggttttgatt tagacggtcg tatggtagaa attgctagaa aaaatgcaga
     1861 agaagcagga cttggagatg ttatcaaatt gaaacaaatg cgtcttcaag atttaaaaac
     1921 agataaaatt aacggtgtta ttatctccaa cccaccatat ggtgaacgac tcctggatga
     1981 caaagcagtg gacattttgt ataatgagat gggacaaaca tttgcgcctt taaaaacttg
     2041 gagcaagttt atcctaacta gtgacgaagg atttgaaaaa aaatacggct cacaagctga
     2101 taaaaaacgt aagctttaca atggtacttt aaaagttgat ttgtaccaat attacggtga
     2161 acgtgtgaga cgtcaagtaa aataacgggg agaactcatg tcagaagatc aaaaacaccc
     2221 attttttgaa ccagaaaaag aaactgatgg tcttgaattt aaagatgcta aagaaatgac
     2281 agtagaagag gctgttagaa aagattctga aattaaggct ggtattaccg aagaagatag
     2341 tattcttgat aaatacatca aacaacatcg tgatgaagta gcttcacaaa agtttgagac
     2401 aaaatcaagc gattttgcaa accttgatac ggcatcttta gatgatttta tcaagaaaca
     2461 gcgcgaagaa ttatcagcga tgttagctgc agaagagctt tctaaaaagt tggataattc
     2521 agtctctcaa gaacaagata tagaagctaa tgctgtctca cctaaagaag aatcttcaca
     2581 agaacaagaa aatagtgtaa ctccagttcc tcctctgaat acggaagcag aaccaactgc
     2641 aacagaacca gattcgacta ttgctgattc agaggagtat aaatcctctt ctaagaagcg
     2701 tggaggtatt gtaggaacat tgattgctct tattttactt ctaatagtaa caatctttgg
     2761 ttacaactat ttcaagaata ataattcgac aaattctcaa actgcaacaa gtcaaagttc
     2821 tagttctaag gctacgacga cttcatcaga agaagataaa aaagctagtc agaacttaga
     2881 taatttcaac aaagcttatg ctaacttttt tgttgatgat aagaaaaccc aactcaaaaa
     2941 tagcgaattt gataaattgt cagaattaga aaagaaagtt gatgctttaa aaggaactaa
     3001 atactacggt aaagtaaaag ttaaatttga tagtctaaaa cgtcaaattg atgctgttaa
     3061 agctgttaac gacaagttta agtcgcctgc tgtagttgat ggtaagaaga gtgaaaaatt
     3121 agaagtaaaa gatggagcga attttgactc tttagatagt aaaactctta atactggtaa
     3181 tgctagtcta gatagtttac ttcactccat cgtttcaact ggtcgtaatc aggtaaaaca
     3241 atccgaagaa caggctaaaa aaactcaaga agaacagtct tcgtctaata aggtttcaga
     3301 cactcaaaca actgaacaac caaacgtaac taacggtcaa tcttcctcat cagctgctac
     3361 aataaataat caggcagctg gcactgcatc aggtaatttg gaacgtaacc gtagccgagt
     3421 accgtataat aatgctgcta ttgcggacac aggaaatcct gcctggatat ttaacccagg
     3481 agtattagag aaaatcgtgg ccacttcaca ggcgcgaggc tatttctctg gaaataacta
     3541 tattcttgaa cctgtaaata ttatcaatgg taatggttat tacaatatgt ttaaattaga
     3601 tggcacttat ttgttctcta tcaatgccaa gacaggttac tttgtaggta acgctcctgg
     3661 aagagctgat agtttagatt attaatataa agagcaaaac cagttttttg tctacaccct
     3721 aaaagttaga aaatcagtct aacgattagg gtgttttttg tattcgcatt tagatgtgta
     3781 cgactgctat gaggatatta gggaaaaatt aaaaggattt tgctctctat gatacagaac
     3841 aaaatccttt gtttaaatta ataagacaac ttttggaatt ccaaaagttg acttttattt
     3901 tttattagat cagacaatgt ggcgctcaaa agcattgtca gaaatccctt gggaaagaat
     3961 taatttcgcc cattcttgag cagagtggag actgtggtct ttataatttc cacaagattc
     4021 aatggttgtt ccaggaacgt cttcccaagt gaccccacca gcaatagcct ctagactaga
     4081 tttgatgact ttagcaattt cagtagcatc ctgttttccc cacataatca tgtgaaaacc
     4141 agttctacaa ccaaaaggag aacaatcaat caaaccatta atttgttggc ggattaactt
     4201 cgccagaagg tgctcgatag tatgtaaacc agcagtatca atagcatttt cattaggctg
     4261 aataaggcgg atatcaaagt tagtaatgat gtctccgacc ggtccaactt cttctgaaat
     4321 taaacgaacg tagggtgctt taacgatggt gtgatctaat tcaaaactct ctacgacaac
     4381 ttcttttgtc ataagttttc tccttttgta ttacattcta gagactaaag gagatgttga
     4441 aaatttctcc taatcccttt aaataacatt ctaatcatat cataaatgtc tataatttta
     4501 aattgtgaaa acgccttttt tatgataaac ttaaaagtgg actttctata tgaaatctag
     4561 aaaaaaattg aggtataaac atgtttaata ttatattagc aatggtttgt gcactcattg
     4621 gtttgattat tggttatgtg gctatttcaa tgaaaatgaa atcatcaaaa gaagctgcag
     4681 aactaactct tttaaatgca gaacaagatg ccgttgattt acgtggtaaa gcagaaattg
     4741 aagcagaaca catccgtaaa gcagcggaac gtgaaagtaa agctcaccaa aaagaattac
     4801 ttttagaagc aaaagaagag gctagaaaat atcgtgaaga gatcgaaaag gaatttaaat
     4861 ctgatagaca agagttgaaa cagatggagg cacgtttaac agatcgtgca tcatctcttg
     4921 atcgtaaaga tgaaaatcta tctaacaaag aaaaaatgtt agatagcaaa gaacaaagtc
     4981 taaccgataa atctagacat attaatgagc gcgaacagga aattgctact ttagaaacaa
     5041 aaaaagtaga agaattgtca cgcatcgcag agcttagtca agaagaagct aaagatatca
     5101 ttttagctga taccgaaaaa gatttagctc atgatattgc gacacgtatt aaagaagcag
     5161 aacgggaagt aaaagatcga tcaaacaaga tagcaaaaga cttgctagcc caagctatgc
     5221 aacgtttagc tggtgaatat gttactgaac aaacaattac aactgttcat ctccctgatg
     5281 ataatatgaa gggacgtatc attggtcgtg aaggacgtaa tattcgtact ctggaaagtt
     5341 tgacaggtat tgatgttatc atagatgata cgccagaagt cgttgtctta tcaggtttcg
     5401 accccattcg tcgtgaaatt gctcgtatga ctttagaatc tttgattcaa gatggacgta
     5461 tccatccagc tcgtattgaa gagttggttg aaaaaaatcg tttggaaatg gatcaacgta
     5521 tccgtgagta tggtgaggca gcagcttatg agattggtgc accaaatctg catcctgatt
     5581 tgataaaaat tatgggacgt ttgcagttcc gtacatcata tggtcaaaat gtacttcgtc
     5641 attcagttga agttggtaaa cttgcaggta ttttagctgg agaactagga gaaaatgttg
     5701 atttagctcg tcgggctggc ttcctccatg atatggggaa agctattgat cgcgaagttg
     5761 aaggcagtca cgttgaaatt ggtatggaat ttgctcgtaa gtacaaagag catccgattg
     5821 ttgttaatac tattgcaagc caccatggtg atgtagaacc tgacagcgtt attgcagtaa
     5881 ttgttgctgc tgcagacgcc cttagttcag cacgaccagg tgcgcgtaat gagtcaatgg
     5941 aaaattatat caaacgtctg cgtgatttag aagaaattgc taatggcttt gagggagtgc
     6001 aaaatgcctt tgcacttcaa gccggacgtg aaattcgtat catggtacaa cctggtaaag
     6061 tttctgatga tcaagtagtt attatgtcgc ataaggtacg tgaaaaaatc gaacaaaact
     6121 tggattatcc aggtaatatt aaggtaacag ttattcgtga aatgcgagct gttgattttg
     6181 caaaatagtc aaaaggagag cttgttagct ctccttttgt tatagagttg acatttgtca
     6241 tattttgaaa tgacaaaaat atctaaaaag tttccttctg attttttata ctatgattat
     6301 agtaaaggag aaccaaatgg caaaagatat aattaaggtt gatcatatat ttaaaagcat
     6361 cgggcaaaaa acgattttag aagatatatc atttagtata gcttctaacc aatgtgtagc
     6421 attgattgga cctaatggtg cagggaaaac aactctgatg tcaactttat taggtgatat
     6481 tagtatttct tcaggagcat tgactatttt taatctgcct gcacaccaca ataggttaaa
     6541 atataaggtg gccatcttac cacaagaaaa tgtattgccc tcaaagttta cagtgagaga
     6601 actcattgat ttccaaaggt gtttatttcc agaagtgctg ccaatgtctc tgattttaga
     6661 ctatctacag tggagtgatg cgcacttgca acaatttaca gagactttat ctggagggca
     6721 aaaacgactt ttagcttttg ttttaacttt ggttggtaag ccccaattac tatttttaga
     6781 tgaacctact tccggaatgg atacttccac acgtcaacga ttttgggagc tggttgcgac
     6841 actaaaaaaa gaaggtgaca caattgtcta ttctagtcat tatctcgaag aggtagaaca
     6901 tacagctgat aggattttag tacttcataa aggaaagtta ttacgcgata caaccccctt
     6961 tgccatgaag caagaaaaaa ccgaaaagtt attcaccgtt ccgcttagtt atcaaaaatt
     7021 attacctacc tatttgatta cagagtgtga agccaagagt gatagtataa cgtttgttac
     7081 tggggaggct gaaactgtat ggaaaatact ggcagataat ggttgtccta ttgaagctat
     7141 tgagatgacc aatagaactt tgttaaatcg tatttttgag actactaagg aggtaaaaca
     7201 tgagaatctt taaagcatta ttgaaaatgg aatttctttt gacaaaacgt caacttgcca
     7261 atctcattat ggctataggt atgccagtgg ctttttttct atttttttca ggatttatgg
     7321 gcgaaggact cactaaagcc atcgaagcta tctacgttcg taattatatg ataacgatgg
     7381 caggattctc aagtcttagc tttgcttttt ttacttttcc attttctatg aaagatgatc
     7441 aactctcaaa ccgaatgcaa ttattaagac actcaccagt accaatgtgg caatattatc
     7501 tagcaaaaat tattcgtatt ttattttact attgcctagc aataactgtt gttttcctaa
     7561 cagggcatat tttaaggcaa gttagtatgc caatagaaca atggatgcag agctttttat
     7621 tgctattagg aggagcaaca tgttttattc cttttggatt gctcgtctcc tactttaaaa
     7681 atactgaact aatgtcaatg gtagccaata tttgttatat gtcattagct gttcttggtg
     7741 gcatgtggat gccaattaca atgttcccaa aatggttgca aggtttatca aagttaaccc
     7801 ctacttacca tttgactcaa gttatcctta gtccgtttgc taatagtttt gctgtttttt
     7861 ctcttattat tctgataggc tatggtatta taatgttagt tatcgcttat ctactatctc
     7921 agaagaggca ttcaatttga aaaataaaaa aaataactat gatttaatgt attttattcc
     7981 gttagt
//
LOCUS       DATRQA010000088         7979 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.088, whole genome shotgun sequence.
ACCESSION   DATRQA010000088 DATRQA010000000
VERSION     DATRQA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.088"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..111)
                     /locus_tag="VBD27_001434"
     CDS             complement(<1..111)
                     /locus_tag="VBD27_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HEO7025439.1"
                     /translation="MNKQERLGMSVYLYYNRDARKLYKYGDVHYHSRRLRY"
     gene            complement(211..801)
                     /locus_tag="VBD27_001435"
     CDS             complement(211..801)
                     /locus_tag="VBD27_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEO7025440.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGQVEDNMANSIIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKSDVQTIVMGMAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQSDMAIAAEHLLKTRHTLEKILADNSGQ
                     SIEKVHDDAERDRWMSAQETLDYGFIDAIMENNNLQ"
     gene            complement(926..1555)
                     /gene="upp"
                     /locus_tag="VBD27_001436"
     CDS             complement(926..1555)
                     /gene="upp"
                     /locus_tag="VBD27_001436"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO7025441.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTTTSTKDFRELVDEIAMLMGYEV
                     SRDLPLEDVEIQTPVATTVQKQLAGKKLAIVPILRAGIGMVDGFLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGAASIKFVCL
                     VAAPEGVAALQEAHPDVDIYTAALDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(1665..2834)
                     /locus_tag="VBD27_001437"
     CDS             complement(1665..2834)
                     /locus_tag="VBD27_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7025442.1"
                     /translation="MITYDFTSLPERFSSNAIKWKAVQKDQEILPLWIADMDFPIFPE
                     MSEAIEDFSHQMVFGYDSPKDSLYQAISNWEVQEHGYQFDKKSLLLIDGVVPAISVAI
                     QAFTKEGDAVLINTPVYPPFARTIKYNNRHLVSNSLLNNNQYFEIDFKQLEKDIIENN
                     VKLYIFCSPHNPGGRVWTKGEIQKIGDICKRYNVILVSDEIHQDLVLFDNVHHSFNTV
                     DSSFKELSVILSSATKTFNIAGTKNSFAIIENEKLRSDFKKRQIANNQQEISSLGLLA
                     TEVAFTKGKQWLKALKMELEGSIEYLYEQLTQKTHIKVMKPEGTYLVWLDFSAYNLTH
                     LEIQEKLRYDAKLILNDGLTFGKEGKKHARINVAAPRSVIEEAVLRLVTNFRKND"
     gene            complement(2970..3518)
                     /locus_tag="VBD27_001438"
     CDS             complement(2970..3518)
                     /locus_tag="VBD27_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEO7025443.1"
                     /translation="MNIETLTQKNHRSDSGRNHIVLFEPQIPANTGNIARTCAATNAP
                     LHIIRPMGFPIDDKKMKRAGLDYWDKLDVSFYDGLEEFMLSCRGKVHLISKFADKVYS
                     DENYNDDQDHYFMFGREDKGLPETFMREHAEKALRIPMNDEHVRSLNVSNTVCMIVYE
                     ALRQQSFPNLELSHTYENDKLK"
     gene            complement(3691..5043)
                     /locus_tag="VBD27_001439"
     CDS             complement(3691..5043)
                     /locus_tag="VBD27_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO7025444.1"
                     /translation="MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDV
                     FLGMVILVLGSFSIVFGSLSISELAIRTSESGGIFSYYEKYVSPALAATLGLFASFLY
                     LPTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQSLTTFV
                     KMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVNI
                     APEVKNPKKNLPLAFVIGPALILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIF
                     GPSVGRLLSFIVILSVLGVANGLLLGTMRLPQAFAERGWIKSERMANINLKYQMSLPA
                     SLTVTAVAIFWLFVHFMVTKFNLLPGSDISEIAVVFNNTSLIILYVLVLSLYLKKDIK
                     NKFTGLVSPILAILGGLILLIGSLLSNFFTVLIFQCFCLLFCLICHYIYQKNNPKTHE
                     "
     gene            5249..6598
                     /gene="trkA"
                     /locus_tag="VBD27_001440"
     CDS             5249..6598
                     /gene="trkA"
                     /locus_tag="VBD27_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEO7025445.1"
                     /translation="MRIIVVGGGKVGTALCRSLVAEKHDVVLIEKKENVLKRVTKQHD
                     IMGIVGNGANYKILEQAEVKNCDIFIAITDRDEVNMISAVLAKKMGAKETVVRMRNPE
                     YSNPYFKDKNFLGFSSVVNPELLAAQYIANTIEFPNATSVEHFANGRVMLMEFKILEG
                     NKLCHTSMSQIRKKFGNIVICAIERDGKLIIPDGDATIQVKDKIFVTGNRIEMILFHN
                     YVKNKVVKNLMVIGAGRIAYYLLNILKNTNTHVKLVELNQEQAEYFSQEFPNVPVVHG
                     DGTAKNILLEESVTNFDAVATLTGVDEENIITSMFLESIGIPKNITKVNRTSLLEIID
                     DKQLSSIITPKRIAVDHVMHFVRGRVNAQDSNLEAMHHIANDRIETLQFEIKETSKLA
                     NRSLASLKLKQNILIAAIIRNNKTIFPTGEDVLTVGDRIVVITLLKNITRTSDMLAR"
     gene            6603..>7979
                     /locus_tag="VBD27_001441"
     CDS             6603..>7979
                     /locus_tag="VBD27_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO7025446.1"
                     /translation="MNKSMIRFLLSKLLLIEAALLAIPLTVGLIYREPQSVMMSIVIT
                     MIILIILGLLGSLFKPKNYHIYTKEGMLIVALCWILWSFFGALPFVISGQIPNIIDAF
                     FEVSSGFTTTGATILDDVSVLSPALLFWRSFTHLIGGMGVLVFALAIMENSKNSHLEV
                     MRAEVPGPVFGKVVSKLKKTAQILYLLYLLMFAVFAVILYFAGMPFFDSIIIAMGTAG
                     TGGFAVYNDSIAHYNSPLITNLVSIGMLIFGVNFNLYYLLLLRKIKAFFGDEELKTYL
                     RIVAIATFMIALNVIGMYDNFRQGLEHIFFEVSAIITTTGFGVTDITRWPLFSQVILL
                     FLMFIGGSAGSTAGGFKVMRSLILAKIARNQVLSTLYPNRVMSLHINKSVLDKNTQHG
                     VLKYLTIYLAIFMALVLVLTLDTNDFLVVISAAASCFNNIGPLLGSNETFSFFSPFSK
                     LLLSFAMIA"
BASE COUNT         2594 a         1514 c         1263 g         2608 t
ORIGIN      
        1 atatcttaat ctacgagaat gataatgaac atctccgtat ttatataatt tacgagcgtc
       61 acgattatag taaagataaa cgctcattcc taaacgctct tgtttattca ataatttttt
      121 cctttctgcg tcatatctgt aattactaag attataacat aattccaaca aataaaaaaa
      181 gagttggtaa actcaactct tttaaatcta ttattgtaaa ttattatttt ccataatagc
      241 atcaataaag ccataatcaa gtgtttcttg agcactcatc caacgatcac gctctgcatc
      301 atcatggact ttttcaatag attgaccaga attatcagct aagatttttt ctaaagtatg
      361 acgcgttttt aaaagatgct cagcagcgat agccatatca gattgctgtg tacctccgcc
      421 tgttccgccc attggttggt ggatcatata ttctgcattc ggtaacataa aacgttttcc
      481 tttagcacca cttgaagcaa taatggttcc catcgaagca gccatcccca taacaatcgt
      541 ctgtacgtcc gatttaatga agttcatggt gtccacaata gcaagtccag ccgatactga
      601 accacctggt gtattgacat aaaggtaaat atcctttgta ttatcttgtg catcgagaaa
      661 caataactgt gcaatgatac tattggccat attatcctca acttggcctg tcaacataat
      721 aatacgatct tttaaaagac gtgagtaaat atcataagaa cgttcaccac gacttgtttg
      781 ttcaataact actggaatca tatctttctc catttctttt tatcactgtc actattataa
      841 tcatttggtc aacaaaggtc aaataataac ttttttaaga aaaggctttc tgagttcttc
      901 agaaaagcct tattcatctc aaatcttatt ttgtaccaaa taagcgatcc ccagcatctc
      961 cgagtccagg aacaatgtag ccatgttcat tgagtttttc atccaaagca gcggtataaa
     1021 tatctacatc tggatgtgct tcttgaagtg ctgccactcc ttctggagct gctactaaac
     1081 aaacgaattt gatactagct gcgccacgtt tttttagaga atctactgct aaaatagcag
     1141 acccacctgt agcaagcatt ggatcaacta caaaaatctg acgttgatca atatcttcag
     1201 gtaacttaac taaatattcg actggttgga aagtctcttc atcacgatac ataccgatat
     1261 gaccaacttt agcagctggc acaaggctta agaaaccatc aaccatacca atacctgcac
     1321 gtaaaattgg aacaattgct aactttttcc cagctaattg tttttgtaca gttgtcgcta
     1381 ctggtgtttg gatttcaaca tcttctaaag gcaaatcacg agacacttca tagcccatta
     1441 acatcgcaat ctcatcaact aactcacgaa aatctttagt agatgttgta gtgcgacgta
     1501 agattgacag tttatgttga ataagtggat gtgaaatgac ttgaaatttt cccattataa
     1561 tgaccgcttt ctatttaatt ttgagcatta aaaacattct aatgcttgtt tacattcaaa
     1621 gtattataac ataaaaaccc tttccatgcg acttttttta tagtctagtc attttttcta
     1681 aagtttgtca ctaatcgcaa aactgcctcc tcaataacag acctaggagc tgcgacattg
     1741 attctagcat gttttttccc ttcttttcca aaagttaaac catcatttaa tattaatttg
     1801 gcatcatatc gaagtttttc ttgaatttct aaatgcgtta aattataagc agaaaaatct
     1861 aaccaaacaa gataagttcc ctctggtttc atcactttga tatgagtttt ttgcgttaat
     1921 tgctcataaa ggtattctat tgatccttct aactccattt ttagggcctt taaccattgt
     1981 ttcccttttg taaaggcaac ttctgtagct aataacccca atgaagaaat ttcttgttga
     2041 ttgttagcta tttgcctctt cttaaaatcc gagcgtaact tttcattttc aattattgca
     2101 aaactatttt tagtccctgc aatattaaac gttttagttg cactagataa aataacactc
     2161 aattctttaa aactagaatc aacagtatta aagctatggt gtacattatc aaataatacc
     2221 aaatcttgat gtatttcgtc agaaacgagg ataacattat atcgtttaca gatatctcct
     2281 attttttgaa tttctccttt tgtccagaca cgcccaccgg gattgtgtgg actacaaaaa
     2341 atataaagct ttacattatt ttcaatgata tctttttcta attgtttaaa gtcaatctca
     2401 aaatactgat tattattaag taaactattt gaaactaaat gacgattatt gtattttata
     2461 gttctagcaa agggcggata gactggggta ttaattagaa cagcatctcc ctcctttgtg
     2521 aaggcttgta tagcaactga aatcgctggt acaaccccat caattaataa taatgatttt
     2581 ttatcaaatt gataaccatg ttcttgcact tcccaattag agatagcttg gtataaactg
     2641 tctttaggac tatcatagcc aaaaaccatt tggtgtgaaa aatcttcaat agcctccgac
     2701 atttctggaa agataggaaa atccatatcc gcaatccaaa ggggaaggat ttcttgatct
     2761 ttttgaaccg ccttccattt tatggcatta cttgaaaaac gttctggtaa acttgtgaaa
     2821 tcatatgtaa tcataattac tcctgactat tctacagcta aaaatatccc ttgtcgcctt
     2881 ggtgactatt ttaccatttg tttttgatac tatatactaa ttaacattct aaagataaaa
     2941 gtgaccattt taaacgatca ctttctttat tattttaatt tatcattttc ataagtatgg
     3001 cttaattcta aattaggaaa gctttgctgc ctaagggctt cataaacaat catacaaaca
     3061 gtgtttgaaa cattcaagct acgcacatgc tcatcgttca tgggaattcg taaggccttt
     3121 tcggcatgct cgcgcataaa agtttctggc aatcctttat cttcacgacc aaacataaaa
     3181 taatggtctt gatcatcatt gtaattttca tctgagtaaa ccttgtcagc aaatttgcta
     3241 attaggtgaa ctttccctcg acatgacaac ataaattctt ccaaaccatc atagaaacta
     3301 acatctagct tatcccagta atccagtccc gctcgtttca tttttttatc atcaatagga
     3361 aatcccatcg gtcgtatgat gtgcaaagga gcatttgttg ccgcacaagt tctagcaata
     3421 ttaccagtat ttgctggaat ctgtggttca aataaaacaa tatgatttcg tccgctatcg
     3481 gaacgatgat ttttctgcgt tagtgtttca atattcatct catcatcact ttctagaact
     3541 cattctgact cgcaaaaaaa ccacactgct cggagtcaat ctcagcaaac aatgtggttt
     3601 atggtggttt gttaacttaa atcataacag gtttgattta aactaacaaa tcgttgacac
     3661 tattatagca cttcagtcta caaagtcaac ctattcatga gtttttgggt tatttttttg
     3721 atagatataa tgacaaatca aacagaataa taagcaaaaa cattggaaaa ttaaaaccgt
     3781 aaagaaatta cttaacaaac tcccgataag gaggatcaaa ccgcctaaaa ttgctagaat
     3841 cggtgaaact aagccggtaa atttattttt aatatctttt ttgagataaa ggctgagtac
     3901 taaaacataa agaatgatta aactggtatt attaaacacc acagcaattt cactaatatc
     3961 actacccggc aataagttaa attttgtaac cataaaatga acaaaaagcc aaaaaatagc
     4021 tacagccgtc accgtcaaac tagctggcaa tgacatttga tacttcaaat ttatgttagc
     4081 catcctctca cttttgatcc atccgcgttc cgcaaaagct tgaggtagac gcatcgtccc
     4141 aagtaaaagt ccattggcaa cacctaatac agatagaata acaatgaaag ataataaacg
     4201 cccaacacta ggaccgaaaa taatattagc tgcataatta atagcatcat taccggttgt
     4261 cataataaag ctagcaccta aaatttgtgt caaaccatag aaaaatgcca aatatgataa
     4321 taagataagc gctggaccaa tgacaaaggc aagtggtaaa ttcttcttgg gattcttgac
     4381 ttctggtgca atattaacaa aaattgtcca accatcatat gcaaaataca atggtaccag
     4441 acctgacacc caactccatc caacatttga cggttgaata gctgttaaat gttgtggaag
     4501 ttgtggagct ttatcagacc aaaaagcacc aattaaagca attagaacta aaggaatcat
     4561 ttttacaaag gtcgttaaag attggaatcc ccctgcaata cgcttagcaa agatattcat
     4621 gagactaagc gctaaaatat aaacagcagc taaaataatc tgtgattcca aacttgatga
     4681 ttcacctaaa gtataaaatg cagctaccca tgaaacgatt gctgttaaag ttggaagata
     4741 caaaaaagac gcaaacaagc ctaaagttgc tgctaaagct gggctaacat atttttcgta
     4801 gtaagaaaaa ataccaccac tttcactcgt tctaatagct agctcagaaa ttgacaaact
     4861 tccaaaaaca atcgaaaaag aacctagtac taaaattacc atgcctaaaa agacatctcc
     4921 accagtaaat ttaaggatgt catcaacttt aaaataaatg cctgaaccaa tcactacacc
     4981 tacaatcata gcaattgtag tcattaatcc ataggtctgt ttaatagaat tatgattctc
     5041 cataatatct ccatttctac tgtacgctta aaggttaatt ataacctaat ttagggcttg
     5101 tcacaagttt atctgtgtta tttttcacca gattctgata agagaatcat attactctta
     5161 tcagaaagat gaaaacgttt tttaaccata attctcgtgc atttttacta aaaaaaaggt
     5221 atcatagagt ttattattgg aggtaaaaat gagaataatt gttgtcggcg gaggtaaagt
     5281 cggaaccgca ctttgtcgct cacttgttgc tgaaaagcac gatgttgttt taattgagaa
     5341 aaaagaaaac gtccttaaac gtgtcactaa acagcatgat attatgggaa ttgttgggaa
     5401 tggagcaaac tataaaattt tagaacaagc cgaagtcaaa aattgtgata tttttatcgc
     5461 tatcactgat cgagatgaag ttaatatgat ttcagcagtt cttgccaaaa aaatgggggc
     5521 aaaagagact gttgttcgca tgcgtaatcc tgagtattct aatccttact tcaaagataa
     5581 aaactttctt ggcttctctt cagttgtcaa ccctgaattg ttagcagcac aatatatcgc
     5641 taataccata gaatttccaa atgcaacttc agttgaacac ttcgccaacg ggcgtgtgat
     5701 gttaatggaa tttaaaattt tagaaggaaa taaactctgt catacttcaa tgagtcaaat
     5761 tcgtaaaaaa tttggtaata ttgttatttg cgcaattgaa cgcgatggca aattgattat
     5821 tcctgatggc gatgcaacta tccaagtcaa agataaaatc tttgtaactg gaaatcgtat
     5881 tgaaatgata cttttccata attacgttaa aaataaagtt gtcaaaaact tgatggttat
     5941 cggtgcagga cgcatcgcct actacctact caatatcctt aaaaatacca atacacacgt
     6001 caaactagtt gaactcaacc aagagcaagc tgaatatttt agtcaagaat ttcccaatgt
     6061 tcctgtcgtt catggggatg gtactgctaa aaatattttg ttagaagaaa gcgttacaaa
     6121 ttttgacgca gttgctactt tgactggtgt tgatgaagaa aatatcatta cctcaatgtt
     6181 cttagaaagt atcggtatcc ctaaaaacat taccaaagtt aatcgaacta gcctactaga
     6241 aatcattgac gataaacaac tatcaagcat tatcacaccc aagcgtattg ccgtagacca
     6301 tgtgatgcac tttgtacgcg ggcgtgtaaa tgctcaggat tccaacctag aagctatgca
     6361 tcatattgct aacgatcgca ttgaaacctt acaatttgaa ataaaagaaa ctagcaaatt
     6421 agcaaatcgt agccttgcat ctttaaaatt aaagcaaaat attttaattg cagcgattat
     6481 tcgcaataat aaaacgattt tcccaactgg tgaggatgtt ttaacagtgg gtgatcgtat
     6541 cgttgttatc accttattaa aaaatattac tagaactagt gatatgttag cgaggtagga
     6601 atatgaataa aagtatgatt cgtttccttc tctcaaaact tttgctaatt gaagctgctc
     6661 ttctagcgat tcctctaact gttggactta tttaccgtga accgcaaagt gtcatgatga
     6721 gtatagtcat taccatgatt attttaatca tccttggatt actgggttct ctctttaaac
     6781 ctaaaaatta tcatatctac actaaagaag gcatgttaat tgttgcactc tgctggatac
     6841 tttggtcctt ctttggcgcc cttccatttg ttatttcagg gcaaatccct aatattatcg
     6901 atgctttctt tgaagtatct tctggtttta cgacaacagg agcaactatt cttgatgacg
     6961 tcagtgtctt atcacccgcg ctcttatttt ggcgtagctt tacccatctt atcggtggaa
     7021 tgggggtttt agtatttgcc cttgccatca tggaaaattc taaaaatagc catttagagg
     7081 ttatgagagc tgaagttcct ggacctgtat ttggtaaagt tgtctcgaaa cttaaaaaaa
     7141 ctgcacaaat tttgtatttg ttatatcttc ttatgtttgc agtatttgca gtaatattat
     7201 attttgcagg tatgcctttc tttgatagta ttatcatagc gatgggaact gcaggtacag
     7261 gtggttttgc agtttacaat gatagcattg ctcattataa tagtcctctg ataactaact
     7321 tagtttctat tggtatgctt atcttcgggg ttaactttaa tctttactat cttcttttac
     7381 tccgaaaaat taaagcattc tttggtgatg aggaactaaa aacctatctc cgcatcgtgg
     7441 caatagcaac tttcatgatt gctctaaacg tcatcggtat gtatgataat tttcgacaag
     7501 gcctcgaaca tattttcttt gaagtatcag ctattattac gacaactggt tttggcgtga
     7561 ctgatatcac tcgttggcct ttgttttcac aggttatttt gctattcttg atgtttatcg
     7621 gtggttctgc tggctctaca gctgggggct ttaaagtcat gcgtagctta atcctagcaa
     7681 agattgcacg taaccaagta ttgtctacac tctatcctaa tagagtcatg tcactacaca
     7741 ttaataaaag cgttctagat aaaaatacac aacatggtgt tttaaaatat ctaactatct
     7801 atttagctat cttcatggca ctcgttcttg ttttaacgct tgatactaat gattttttgg
     7861 ttgttatcag tgctgcagcc tcatgtttta acaacatagg cccactctta ggtagcaatg
     7921 aaactttctc attctttagt ccattctcaa aactccttct ttcatttgct atgatagca
//
LOCUS       DATRQA010000089         7959 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.089, whole genome shotgun sequence.
ACCESSION   DATRQA010000089 DATRQA010000000
VERSION     DATRQA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.089"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..487
                     /locus_tag="VBD27_001442"
     CDS             <1..487
                     /locus_tag="VBD27_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7025447.1"
                     /translation="YIEFVRNVPNLLWIFIIFLVFQMKSVSAGITSFTIFTSAALAEI
                     IRGGLNGVDKGQTEAGLSQGFTYLQVFIIIIFPQAFRKMLPAIISQFVTVIKDTSLLY
                     SVIAIQEIFGKSQILMGRYFEAGQVFTLYAIITAVYFITNFIISSFSRKLSKRWEQAT
                     E"
     gene            complement(705..1898)
                     /locus_tag="VBD27_001443"
     CDS             complement(705..1898)
                     /locus_tag="VBD27_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7025448.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKTSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNEQRANVKKST
                     MKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKEKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKNGTRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFVNHKGKPKVAIDYNALFVRMVKKYNKQHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(1977..2183)
                     /locus_tag="VBD27_001444"
     CDS             complement(1977..2183)
                     /locus_tag="VBD27_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO7025449.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(2176..3318)
                     /locus_tag="VBD27_001445"
     CDS             complement(2176..3318)
                     /locus_tag="VBD27_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO7025450.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            3830..4465
                     /locus_tag="VBD27_001446"
     CDS             3830..4465
                     /locus_tag="VBD27_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7025451.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            4619..4696
                     /locus_tag="VBD27_001447"
                     /pseudo
     CDS             4619..4696
                     /locus_tag="VBD27_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            4941..5057
                     /locus_tag="VBD27_001448"
     CDS             4941..5057
                     /locus_tag="VBD27_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO7025452.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5073..6992
                     /gene="tet(M)"
                     /locus_tag="VBD27_001449"
     CDS             5073..6992
                     /gene="tet(M)"
                     /locus_tag="VBD27_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO7025453.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7111..7278
                     /locus_tag="VBD27_001450"
     CDS             7111..7278
                     /locus_tag="VBD27_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO7025454.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7338..7691)
                     /locus_tag="VBD27_001451"
     CDS             complement(7338..7691)
                     /locus_tag="VBD27_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7025455.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         2393 a         1442 c         1526 g         2598 t
ORIGIN      
        1 ctatatcgaa tttgtcagaa atgttccaaa tctactttgg atttttatca ttttcttagt
       61 attccagatg aaatcagtca gcgctggtat tacttcgttt acaattttta cctccgctgc
      121 tttagcagaa attattcggg gaggtttaaa tggagttgat aaaggacaga cagaagctgg
      181 actttctcaa ggattcactt atttgcaagt tttcattatt attatttttc cacaagcttt
      241 tcgcaaaatg ttgccagcca ttatttctca gtttgtaacg gttataaaag atacatcact
      301 actttattct gttatagcta ttcaagaaat cttcggaaaa agtcaaattc taatgggaag
      361 atatttcgaa gctggacaag tttttacact ctacgctatt ataactgctg tctatttcat
      421 tacaaacttt atcatctcaa gtttttcacg taaactttca aaacgttggg agcaagctac
      481 tgagtaatta aaacgaaggc cttttagacc ttcgtttttt tatatcttat ttaaattaaa
      541 ttatatatgc ctttttgact atctttttat atcttctcaa atgtccgcaa agccttattt
      601 tatcggctct acgggcattt tgcttttgtg gtaaacctca catatctagg tctatctttt
      661 tatattttcg ctatcaaacg tggttaaaat cgtggtaaat acttctatgc gattagacgc
      721 tgaacctctg attttgcggt atctatggac gcatgagcat accagttcat tgtgatactg
      781 atgtttgaat gtcccatgat atactgtaaa tctttcgggt tcatgttctt gcttgccagc
      841 cttgtgcaga acgtatggcg tagcgtatgc ggtgtgatat gtggcaaggg attgtccttg
      901 tgttgcttgt tgtatttctt taccatacgg acaaataagg cattgtaatc aatcgcaact
      961 ttgggcttgc ctttatgatt gacaaacaga aaattgctcc gtccgtctat cacaaatggt
     1021 tctgcctttg ggcgtttttt cataacccgt tgaaatgcct gtattgtttc tctgcttaat
     1081 ggcacttgcc ttgttccgtt ctttgtttta ggcgtttcaa tataatagcc ctgttccttg
     1141 ctctttaata actggtggtc gataatcaca acttcattct tgaaatcaat atcggctact
     1201 gtcagtccgc acagttccga gatacgaagt ccagtcttta acagtatcag cacatcatca
     1261 taatacttat gatacacgtt gtccgtctta ataaatgaca gtagggcttg ttcttgttcc
     1321 tctgtcaatg ccactttctc tttggtatca ttttctagga cttcacttaa cttgaaatca
     1381 aaagggttct tccttacaca atcgtcttgt atggcgatat agaatgacgc ttttaacgag
     1441 cgtttatggt tgttaatagt attgtaggaa aagcctttct ctttcatgcg taacgcccat
     1501 tccttagcgt cagagggttt tatcgtatca atgctcctag cacccaactt gtcctctttc
     1561 aataaccgca tgagctgttc ccgttgtttc attgtgcttt tcttcacgtt tgccctctgt
     1621 tcgttctgtt tggcgtagag ttggcaaagc gtcattttct tgcctgtgct gtcgataccg
     1681 tcctcaatat cccgtcttat ctgctgttcc agttcacgaa gtgaggtttt ttcccgtttt
     1741 ccctttggtg tcgggtctgt gggtgtcaat ctccaagcat acacatattt tgtatttcca
     1801 aatgcgtcta catatttata caagtatttt ccgtctgttc gttggctctc tccagtatgc
     1861 aggatacgtc ctttgttatc ccgtcttttt tctttcatgg tgtctgctcc tttccttgtt
     1921 ggaaagagcc ttgatatgac ttgtgttcat cataacacat acaaggctca tttgcattag
     1981 attgcgtcca atttatcaat aacctgttca aactgtcgtc gtttaatctg tatgcggttg
     2041 ccattcataa tgagccagcc agcgtccttg ttttcctctg ccaagcgtct taacttgttt
     2101 tctccgatac ggaaatactt tgacgcttct tcaatggtaa gggtgtattt ttcccatacg
     2161 ggaatatcct gtgcattact cataaaacac cccctcactt tcaattacgt ctgctatgtc
     2221 cgtacacatt tgttccacaa gcatttcctg tttttcagaa aggtgggtac attcatcaat
     2281 cattttcatg tagtttgagc ctgtcagcct gtcgatttcc tctatcatca cgattgacgg
     2341 catgacctgt ctttccagcc attgcagagt gcgttccata ttgacaggta cggggttcat
     2401 ttcaaaaggt aaactatcat gggaaatgaa tatctcccac agcggatagt cgggaaattg
     2461 cacaaaatca gtaataaggt aaaacaccag cccatgaggg ttatacgtca ataacagttc
     2521 ctcaactgcc tgtactgcct ttttgtcacg cagacgaatt tcaaaacggt tgataatatc
     2581 cgtatgtttc tttttagttg cctgttcctt ttgtttttca tacaggcaga aatattttgt
     2641 gcttgctttt gagccgatat aaagggtgct tgctaaattg cggttttttc cgcttagttt
     2701 accgccttgc acattttcat agtttttaca gcgacaatcc gcaccgccat ttttgtattt
     2761 ttcagataaa acgggaatat ccagcagacc gcacatatcg ttgattgcca agtcaaaacg
     2821 tcggataaca ccgccacagt caagacagcg gttcaagaat gaataccaat ccctgttctg
     2881 tgcctgtaaa acatattcca tattgcgtga gcctgcccct tttaattcca aaaatacgcc
     2941 catgtgttca tcatcagaag ccataacctt gatttctccg taggcgtaat gctctttata
     3001 gccataatat ccatagtcat aatggataaa ataatctgct ttcatgccaa gcacctttcg
     3061 gataatttcc aatgcgtcag tcgttggaaa acggacagac agataatcaa cgagcagata
     3121 aaaaggctta tcgcagatat agtaattcag acaacgcagg ataatatttt gtgtttcctt
     3181 gctggcgttg gcttttccgt tctcaaagcg gttgatacta tatcttgata catgggtaag
     3241 acttgcaagt tctccttgtg aaagaccgct tgcaagacgt ttattcttca tttcaagtgc
     3301 aaattctaaa ttgttcattg tattcctcca atcccacaat agccttgaaa tacctgtaaa
     3361 ccactattca aaagaatgtg aaattactat cttcttaaaa ctcctcatta ctccttacag
     3421 ctttggggtt gtatggtttt tgacttgtct tttgtgaaat agtgcccccc tgttagataa
     3481 cgggggcaca gacaaggctc gcaggctcgc cagccgacaa agcatttctt ccccttgaca
     3541 caaggtgtca tggcggtaag cctgagaggc ttccgccacc gtgtcagaaa tactttgtca
     3601 gcaagttctg ttttgccttt tctaacaagt tccatttcaa ccgatattca tatctacgga
     3661 tttaatcaga taggtatttc tttttgaatg tttttatatt tcattctatt gtgcttattc
     3721 gattacaaga acatagtaat tcttatatgc ttatttgtca atatcttttt ttgcctttac
     3781 aaatcatatt aagcatgata gacttataat agcgaagata taacgagata tggagggtta
     3841 tgcaatgaaa ataagccatt taggaaataa tatacagacc ataagaaaat ttaggggaat
     3901 gaaacaacag gaacttgcgg acaaaatcgg tatcaatatg cagagccttt ccaagattga
     3961 aagagggttg aactatcctg cctatgaaac gctggaaaag ataatggaag tgctggacgt
     4021 tacgcccaat gaattgttgt cgggagaatg gaagtatgtc aatcaatctg aaaaggaagt
     4081 ctgtcagttt ttaagagccg aagaacgtct aaatgcagaa ctgaaacatg gacactatga
     4141 taacttcttt gacagcgagg aagaatggct ggaatatgag ttagaaaagt tacgggaata
     4201 tattacagac tatatcaacg ggaaaagcat tgaagcgtcc gacctttacc caatcaaaga
     4261 atttatccag catttgaagt ttcaaaaatt attagaccgc tatgatgatt tatacagtat
     4321 ggatatgttt ggggaaagca tagagggaca taagtataag acaccttatc aagtcgtgaa
     4381 aatgataaac ccgaactcaa aagaagatat ggaactgtta cgagaagtat taaggaataa
     4441 caattttgat gatgaggacg agtaactttt ttcctcttgc gagaagatac agcaaacaaa
     4501 aagcccgatc aagagtgcaa agcaccacca atggtggcaa agctcttgac agggcttttt
     4561 cttttctgtt gtgggcaatc aagaggaaga aagggataaa atctgatttg tggtaaatca
     4621 ggcaaaggca acgcaggtat ctcaatttga tttggtactt gaaaagaacg ggagtaattg
     4681 gaagattata gaataacaaa tattggtaca ttattacagc tattttgtaa tcacgtactc
     4741 tctttgataa aaaattggag attcctttac aaatatgctc ttacgtgcta ttatttaagt
     4801 atctatttaa aaggagttaa taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt
     4861 gatagtggga acaaataatt ggatgtcctt ttttaggagg gcttagtttt ttgtacccag
     4921 tttaagaata cctttatcat gtgattctaa agtatccgga gaatatctgt atgctttgta
     4981 tgcctatggt tatgcataaa aatcccagtg ataagagtat ttatcactgg gatttttatg
     5041 cccttttggg cttttgaatg gaggaaaatc acatgaaaat tattaatatt ggagttttag
     5101 ctcatgttga tgcgggaaaa actaccttaa cagaaagctt attatataac agtggagcga
     5161 ttacagaatt aggaagcgtg gacaaaggta caacgaggac ggataatacg cttttagaac
     5221 gtcagagagg aattacaatt cagacaggaa taacctcttt tcagtgggaa aatacgaagg
     5281 tgaacatcat agacacgcca ggacatatgg atttcttagc agaagtatat cgttcattat
     5341 cagttttaga tggggcaatt ctactgattt ctgcaaaaga tggcgtacaa gcacaaactc
     5401 gtatattatt tcatgcactt aggaaaatgg ggattcccac aatctttttt atcaataaga
     5461 ttgaccaaaa tggaattgat ttatcaacgg tttatcagga tattaaagag aaactttctg
     5521 ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg acgaacttta
     5581 ccgaatctga acaatgggat acggtaatag agggaaacga tgacctttta gagaaatata
     5641 cgtctgggaa attattggaa gcattagaac tcgaacaaga ggaaagcata agatttcata
     5701 attgttccct gttccctgtt tatcacggaa gtgcaaaaaa caatataggg attgataacc
     5761 ttatagaagt gattacgaat aaattttatt catcaacaca tcgaggtcag tctgaacttt
     5821 gcggaaaagt tttcaaaatt gagtattcgg aaaaaagaca gcgtcttgca tatatacgtc
     5881 tttatagtgg cgtactgcat ttgcgagatt cggttagaat atcggaaaag gaaaaaataa
     5941 aaattacaga aatgtatact tcaataaatg gtgaattatg taaaatcgat aaggcttatt
     6001 ccggggaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata
     6061 caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg
     6121 ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag
     6181 atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt
     6241 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
     6301 agatagaaat aaaagagcct acagtcattt atatggaaag accgttaaaa aaagcagagt
     6361 ataccattca catcgaagtt ccaccgaatc ctttctgggc ttccattggt ctatctgtag
     6421 cacagcttcc attagggagc ggagtacagt atgagagctc ggtttctctt ggatacttaa
     6481 atcaatcgtt tcaaaatgca gttatggagg ggatacgcta tggctgtgaa caaggattgt
     6541 atggttggaa tgtgacggac tgtaaaatct gttttaagta tggcttatac tatagccctg
     6601 ttagtacccc agcagatttt cggatgcttg ctcctattgt attggaacaa gtcttaaaaa
     6661 aagctggaac agaattgtta gagccatatc ttagttttaa aatttatgcg ccacaggaat
     6721 atctttcacg agcatacaac gatgctccta aatattgtgc gaacatcgta gacactcaat
     6781 tgaaaaataa tgaggtcatt cttagtggag aaatccctgc tcggtgtatt caagaatatc
     6841 gtagtgattt aactttcttt acaaatggac gtagtgtttg tttaacagag ttaaaagggt
     6901 accatgttac taccggtgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata
     6961 aagtacgata tatgttcaat aaaataactt agtgtatttt atgttgttat ataaatatgg
     7021 tttcttgtta aataagatga aatatttttt aataaagatt tgaattaaag tgtaaaggag
     7081 gagatagtta ttataaacta caagtggata ttgtgtcctg tatgtggaaa taaaacacga
     7141 ttaaagataa gggaagatac tgaattaaaa aaattccccc tctattgtcc gaaatgcaga
     7201 caagaaaatt taattgaaat aaagcagttc aaagtaactg tgattacaga gccagacgca
     7261 aagacgcaga gccgataaaa tgagattaat acaatctcat tttatcggct ctttccgtta
     7321 tgtatggatt cttttaatta gtcttcgatg tttcttgctt cgttgatacc gctggctaaa
     7381 gattccatta aggatagttc tttgtctgta aagctatcca tgtatttctc tatctgtaat
     7441 cgtcgggtgc tttttaccaa gttattagca ggtaagaaaa attcatcaac ggaaacatga
     7501 agtaacgata caaggtcata aagaacttgt atgctggggt gttgcccttt attttcaata
     7561 ttagttaagt accgtgggtc aatttcaatc aatgctccca cttgttcacg agttaaacct
     7621 cgtttcaatc gagcttcttt aatggctaaa ccaaaggctc taaaatcata tttatcttct
     7681 tttttacgca tagtagacca cctctataca ttttattgtt cctactgaat taaaaacagg
     7741 tatagaaaaa cgtgttatat ggtttatagg tttatattta ataaaaagca ctactaaacg
     7801 ccaataaaaa aaaccgttat atggtagtgc tatttacgct gttaaaatat tgtatattac
     7861 ttccaaatgg cggtttgttg gaggtcaacg tcgccatgaa gtacatcata tacaataaat
     7921 ttccttacat tgggttcttg tcaaaaaaag tcgtctatc
//
LOCUS       DATRQA010000090         7932 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.090, whole genome shotgun sequence.
ACCESSION   DATRQA010000090 DATRQA010000000
VERSION     DATRQA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.090"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..70)
                     /locus_tag="VBD27_001452"
     CDS             complement(<1..70)
                     /locus_tag="VBD27_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 domain-containing protein"
                     /protein_id="HEO7025456.1"
                     /translation="MKNFIKQSDTTFVRIIKSLLIGG"
     gene            complement(73..288)
                     /locus_tag="VBD27_001453"
     CDS             complement(73..288)
                     /locus_tag="VBD27_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7025457.1"
                     /translation="MGLILKNRLKELRARDGINQTELAKLTGVSRQTISLIERGEYTP
                     SVIIAMKISQVFKESLENVFSLVSTEE"
     gene            complement(333..626)
                     /locus_tag="VBD27_001454"
     CDS             complement(333..626)
                     /locus_tag="VBD27_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025458.1"
                     /translation="MVMTYIGVPKEQKALSRRGKQLEKINQGGKTLMSQMIQDLVGST
                     CMIYLEDGFQTHPYEILKVDEDWVQIRRNLKRSKEEVRILRIHDIKELRPVNK"
     gene            complement(658..846)
                     /locus_tag="VBD27_001455"
     CDS             complement(658..846)
                     /locus_tag="VBD27_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025459.1"
                     /translation="MKKEFIITGRMVARVMETLVCLCMVSNLVLDMLDIIEINEIVFC
                     VLVASIAGLEFCLQKERE"
     gene            complement(1047..1865)
                     /locus_tag="VBD27_001456"
     CDS             complement(1047..1865)
                     /locus_tag="VBD27_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO7025460.1"
                     /translation="MNLLFSIDDMYVDHFKVMLYSLVRQTKNRKLEIYVLQKTLLKRH
                     TELIQYTQNLEVGYHPIIVGTEVFAQAPTTDRYPDTIYYRLLAHKFLPETLDRILYLD
                     ADMLCLNDFSSLYDMELGDQLYAAASHNTDGKFLDYVNKLRLKNVELESSYFNTGVLL
                     MNLPAIRKVVHQQTILDYIMQNRGRLILPDQDILNGLYANLVKPIPDEIYNYDARYSL
                     IYQLKSRNEWDLEWVINHTVFLHFAGRDKPWKKDYRGRYSGLYKFMAKEALTKN"
     gene            complement(1953..2669)
                     /locus_tag="VBD27_001457"
     CDS             complement(1953..2669)
                     /locus_tag="VBD27_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEO7025461.1"
                     /translation="MVGLGTVINVILIIVGGFVGLFLKNFLKESLQKSLMQAMGVAVL
                     FISISGVLEKMMLVEKSHLISNHTNMMIITLALGTVLGELLSLDSYIDRFGDYLKQKT
                     GSGNDIKFVEAFVTSTCTVCIGAMAVVGSIQDGIAADHSILFAKGMLDMIIIAIMTVS
                     LGKGALFSALPVALLQGSLTIVAFFMGSLLNPSSLDYLNLVGNMLIFCVGVNLLFNLN
                     IKVINMLPAIILAILWGSFI"
     gene            complement(2751..5369)
                     /gene="alaS"
                     /locus_tag="VBD27_001458"
     CDS             complement(2751..5369)
                     /gene="alaS"
                     /locus_tag="VBD27_001458"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO7025462.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDATAKVQALS
                     DSLRDLQKENVELKEKAAAAAAGDVFKDIQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKGLAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAENL"
     gene            complement(5385..5870)
                     /locus_tag="VBD27_001459"
     CDS             complement(5385..5870)
                     /locus_tag="VBD27_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEO7025463.1"
                     /translation="MATFQIKEKMFSLGGKFTITDLTGLPCYHVEGSLFPLPKTFKVF
                     DEEEHLISQIEKKVLSFLPKFNVTLANGNHFTIKKDFSFLKPHYTIEDLDMEVKGNFW
                     DMDFQLLKDNQVIANISQQWFRMTSTYQVEVYSETYNDLTISLVIAIDYVKELEKNAS
                     N"
     gene            complement(6112..7041)
                     /gene="prsA"
                     /locus_tag="VBD27_001460"
     CDS             complement(6112..7041)
                     /gene="prsA"
                     /locus_tag="VBD27_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEO7025464.1"
                     /translation="MKTRSKLAAGFLTLMSVATLAACSGKTSNGTNVVTMKGDTITVS
                     DFYDQVKTSKAAQQSMLTLILSRVFDTQYGDKVSDKKVSEAYNKTAKGYGNSFSSALS
                     QAGLTPEGYKQQIRTTMLVEYAVKEAAKKELTEANYKEAYKNYTPETSVQVIKLDAED
                     KAKSVLKDVKADGADFAKIAKEKTTATDKKVEYKFDSAGTSLPKEVMSAAFKLDKNGV
                     SDVVSTVDSTTYKTSYYIIKVTDKTEKKSDWKSYKNRLKEVILKDKTSDRSFQNKVIS
                     KALEKANVKIKDKAFAGILSQYATTSGSSSLKK"
     gene            complement(7102..7809)
                     /locus_tag="VBD27_001461"
     CDS             complement(7102..7809)
                     /locus_tag="VBD27_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEO7025465.1"
                     /translation="MVQSYSKNANHNMRRPVVKEEIVQYMRQHQKQNNGCLAELEAFA
                     KQENIPIIPHETATYFRFLMQTLQPKHILEIGTAIGFSALLMAENAPEAKITTIDRNE
                     EMIALAKENFAKYDNHNQITLLEGDAVDVLQTLDKSYDFVFMDSAKSKYIVFLPQVLK
                     HLDVGGVVVLDDIFQGGDIAKPIDEVRRGQRTIYRGLQRLFDSTLQHPDLTATLVPLG
                     DGLLMIRKNADHIVLED"
BASE COUNT         2413 a         1598 c         1265 g         2656 t
ORIGIN      
        1 aaccaccaat taaaagactt ttgataattc gaacaaatgt agtatcactt tgttttataa
       61 aatttttcat cgttattcct ccgttgacac taaactaaaa acattctcta gggattcttt
      121 aaatacttga gaaatcttca tagcaataat aacagatggc gtatattctc cgcgttcaat
      181 taggctaatt gtttgtcttg agactcccgt taatttggcg agttctgttt gattgatacc
      241 atctcgtgca cgcaattctt tgagcctgtt ttttaaaatt aatcccatca tttttctcct
      301 ttataaaatg atatatagat ttattcctca ctctatttat tgactggacg caattccttg
      361 atatcatgaa tacggagaat acgtacctcc tctttacttc gtttcaagtt gcgacgaatc
      421 tgtacccaat cctcatctac ctttaaaatc tcataaggat gcgtttgaaa accatcctct
      481 aaataaatca tacatgtgct tcctactaaa tcttgaatca tttgtgacat aagtgttttt
      541 cctccctgat ttattttttc taattgttta cccctacgac tgagtgcctt ttgctcttta
      601 ggtacaccaa tatacgtcat gaccataaaa agccattaag ccaaaaattc catctatcta
      661 ctccctttct ttttgcaaac aaaactcaag ccctgcaatt gatgctacca acacacaaaa
      721 tacaatttca tttatttcta ttatatcaag catatcaaga acaagattac ttaccataca
      781 taagcaaact agggtttcca taactcttgc aaccatccga cctgtaataa taaattcctt
      841 tttcatttga gtaactccta aaaccattat gcttttgaaa caatgattaa tttgattgtt
      901 caatgattaa gtgattttaa cacgctcctc ctttgatatt atttttcttt tttataaata
      961 attattagca aagattattg atatcattaa aatatcaccc caaaaaaagt tactaaatag
     1021 aaataattta gtaacctttt aaatcttcaa tttttagtta aagcctcttt agccataaac
     1081 ttataaaggc ccgagtaacg tcctctataa tcttttttcc atggtttatc tcgtcctgca
     1141 aaatgtaaaa aaacagtatg gttaataacc cactctaaat cccattcatt gcgacttttt
     1201 aattgataaa taagactata cctagcatca taattataaa tttcatcagg tattggttta
     1261 accaaattag cgtataagcc atttaagata tcctgatctg gtaagattag acgaccacgg
     1321 ttttgcatta tgtaatctaa gatagtttgt tgatgaacaa cttttcgtat agcaggcaag
     1381 ttcatcaaga gtacccctgt attgaaataa gaggattcta gctctacatt ttttaaccgt
     1441 aatttattga cataatctag aaacttacca tcagtattat gacttgccgc tgcatatagt
     1501 tgatcaccta actccatgtc atataaactt gagaaatcat ttaagcaaag catatctgca
     1561 tctaaataaa gtatcctatc taaggtctca ggtaaaaatt tatgagccaa aagtcgataa
     1621 tatattgtat ctggatagcg atcagttgta ggtgcctgag caaatacttc ggtacctaca
     1681 attattggat gatagccaac ttctaaattt tgagtatatt gaataagttc tgtatgacgt
     1741 ttgagtaatg ttttttgtaa gacatatatc tctaacttac gattcttggt ttgtcggaca
     1801 agcgaataaa gcatcacttt aaaatgatct acgtacatat catcaataga aaataataga
     1861 ttcatgagtt ggatcctctc cgtagtatat cttctttcat cctaacatat gtctaaagaa
     1921 aaaccaagtt tgagttaaaa cttggttatc ttttatataa aacttcccca taatatagct
     1981 aaaataattg ctggcaacat attgataact ttaatattta ggttaaagag taaattaaca
     2041 ccgacacaga atataagcat atttcctact aagttaaggt aatctaaaga tgatggatta
     2101 agtaggctcc ccatgaaaaa agcaacaatc gtaaggctac cttgtagtaa agcgacagga
     2161 agtgcagaaa atagagcccc tttccctaaa gacactgtca taattgcaat aataatcatg
     2221 tctaacattc ccttagcaaa taaaatcgaa tgatctgctg ctataccatc ttgaatgcta
     2281 ccaacaacgg ccattgctcc aatacaaaca gtacaagttg aggtgacgaa tgcttccaca
     2341 aatttaatat cgttaccact tcctgttttt tgcttaagat agtccccaaa tctatcaata
     2401 tacgaatcta gtgatagcaa ctctccaagt accgtaccta gagcaagagt aataatcatc
     2461 atatttgtat gattagagat gagatgtgac ttttcgacta acatcatttt ctccaaaaca
     2521 cctgatattg aaataaataa aactgcgact cccatggctt gcatgaggct cttttgaagt
     2581 gattctttca aaaaattttt caaaaataaa cctacaaagc ctccaactat aataagaata
     2641 acattgataa ctgttcctaa accaaccata tttctcctat atacacaaag gaacagaacg
     2701 atgttctgcc ctttgcctgt tttatttact aactaaatga gaggtacttc ttataaattt
     2761 tctgcaactg ctgctagcag ctctgcaatt ttacttgcat cgctaccacc tgccatagcc
     2821 atgtctggtt taccaccacc acgacctgct acaattggtg ccaatccttt gatcatgtta
     2881 ccagcgtgga catctttagt tttgcttgca acaaggacat taaccttctc accaatagct
     2941 gctacgagaa caagcacatc agagtagtct ttttgtttcc agttatcagc aaatgtacga
     3001 agtgcacctg cgtctgcaac atcaacttga ctagcaatga agcgcacgcc cttagcttct
     3061 tgaatatctt taaagacatc accagctgct gctgctgctg ctttttcttt aagctctaca
     3121 ttttcttttt gaagatcacg aagtgaatcg ctaagagctt gtactttagc tgttgcatct
     3181 ttcaattgcg gagcttttac agtagcagcg atctctttta gtgcatcctc ttggttacga
     3241 taagcttcaa aagcttgtct accagtaact gcaataatac gacgagtgcc tgaaccaata
     3301 ccttcttctt tgacaatctt gaagagaccg atttctgaag aattatttaa gtgagttcca
     3361 ccgcaaagtt caacagaata attaccaatt tgaaccacac gaaccacttt accatatttc
     3421 tcaccaaaaa gtgccattgc tcccatctct tttgcggttt caacgtcagt ttcagtcgtt
     3481 gtgattgtaa gagcgttcca aatttgctca ttaacttctt gttcaatgtg acgaagttcc
     3541 tcattgctta ctgcttcaaa gtgagtaaaa tcaaagcgca agaattcttc ttcgttcaat
     3601 gaaccagcct gagttgcgtg ttcaccgata acattgtgaa gggctgcatg gagcaagtga
     3661 gttgctgtgt ggtttttctc aacagccaaa cgacgctctt tgttgatttc aagtgtgtag
     3721 tttgttccaa ctgaaagtga tgccaaaacg tttacagtgt gtagaggttg accatttggt
     3781 gctttttgaa catcaacaac ctcagcaact gtgtcaccct tatcattttt gattctacct
     3841 gtgtcagcaa cctgtccacc catttcagca tagaatggtg tttgagcaaa gacaagaagg
     3901 gcttgacctt ctgaaacagc ctcagtacgt tcattatcag caatgataac tgaaagactt
     3961 gattcaagac tatatgtgtc gtattcgaat cgtgattctt caacaatacc agctagggtt
     4021 tcattttgca tccccatcga accaccctta acaacagctg cacgcgcacg gtcttgttgt
     4081 tctttcatgg ctgacttaaa gccttcgtgg tcaatcttat agcctgcatc ttctgccaat
     4141 tcctctgtca attcaactgg gaatccataa gtatcataaa gtttgaagat atctttacct
     4201 tcaagtgtgt ctttaccttc agccttaagc tgcgcaagca atgaatctaa gtgaccgcta
     4261 ccagcatcga tagtacgagc aaatgtttct tcctcacgtt taacgatttt ctcaataaaa
     4321 tcacgttttt caagcacttc tggatagtag ctttccatga tttgtccaac agtcggaacc
     4381 aatttgtaaa ggaaagtttc gttgatgcca agacggcgac cgtgcataac cgcacgacgg
     4441 agaagacgac gaagaacata accacgacct tcatttccag gaagcgcacc atcaccgatg
     4501 gcaaatgaaa gcgcacggat gtgatcagcg ataaccttga agctcatgtt gtcgccatct
     4561 ggatcgtaag ttttacctga caatttctct acttcacgga tgataggcat gaaaaggtca
     4621 gtttcaaagt ttgtttttgc cccttgcata acagctgcaa gacgttcaag acccgcaccc
     4681 gtatcaatgt ttttgtttgg caattctttg tattctgaac gtggtacggc tgggtcagca
     4741 ttgaattgtg agagaacaat gttccagatt tcgatgtaac gatcgttttc gatatcttca
     4801 gccaaaagac gaagtccaat attttctgga tcaaaatctt caccacggtc gaagaaaatc
     4861 tcagtatctg gacctgaagg accagcaccg atttcccaga agttatcctc gattggcacc
     4921 aagtgacttg gttcaacgcc acaagcaatc caacggttat atgaatcctt gtcatctggg
     4981 taataagtca tgtagagctt gtctttaggg aagtcaaacc attcaggact tgtcaagagt
     5041 tcaaatcccc actcaatagc ttcatcacgg aaatagtctc caattgagaa gttaccaagc
     5101 atttcaaaca tagtatggtg acgtgctgtt ttaccaacat tttcaatatc attagtacga
     5161 attgattttt gtgcattggt aatacgtgga ttttctggaa tcactgaacc atcaaaatat
     5221 tttttcaagg ttgcaacacc tgagttgatc caaagaagcg ttgggtcatt aacaggaacc
     5281 aagttagctg aaggctcaac ggaatgtcct ttggatttcc agaaatccaa ccacatttgg
     5341 cggatttgtg cagacgataa ttctttcatt attctctcct aaaattagtt tgatgcattt
     5401 ttttctaact ctttgacata gtcgatcgct atgacaagcg aaattgtcaa atcgttgtaa
     5461 gtttcactgt aaacttctac ctgataagtt gaagtcatac gaaaccactg ttgagatatg
     5521 tttgctataa cttgattgtc ttttaagagt tggaaatcca tatcccaaaa atttccctta
     5581 acttccatat caaggtcttc aatggtgtaa tgaggtttca aaaatgagaa atctttctta
     5641 atcgtgaaat gattcccatt ggctaatgtc acattaaact ttggtaaaaa acttaaaacc
     5701 ttcttctcaa tctgactaat gagatgttcc tcttcgtcaa agactttaaa agtcttaggt
     5761 agagggaaca aacttccttc aacgtgataa cagggaagcc cagtcaggtc tgtaatagta
     5821 aatttaccgc ctagagaaaa cattttttct ttaatttgaa aagtagccat atctcttcct
     5881 cctcgaaagt tgacaaatag caaaagacaa gtccattacg aagggcgaaa accgcggtac
     5941 caccttcatt caatgaactt gtcattctca tttattttta gttttacccc attgagtagc
     6001 atctttttct gattatccat gactcgcagc aaccgtcact tttctgtatc taatcgttga
     6061 aatttcattc tcaatagtat taattataca gtctttttaa gacttggtca actatttttt
     6121 aagtgatgat gaaccacttg ttgtagcata ttgtgataag atgcctgcaa atgctttatc
     6181 tttaatctta acgttagctt tttctaaggc ttttgaaatc actttatttt ggaaggatct
     6241 atcgcttgtt ttatccttaa gaataacttc ttttaagcga tttttgtaag atttccaatc
     6301 agattttttc tctgtcttat cagttacttt aatgatgtag taacttgttt tataagttgt
     6361 tgaatcaacc gttgaaacca catctgaaac accattttta tctagcttaa aggctgctga
     6421 cataacttct ttagggaggc ttgtccctgc agaatcaaat ttatactcaa cttttttatc
     6481 agtagctgtt gttttttctt ttgcaatctt tgcaaaatca gctccatcag cctttacatc
     6541 tttaaggaca gatttagctt tatcttctgc atccaatttg attacttgta cagaagtttc
     6601 aggagtatag tttttatatg cttctttata gtttgcttct gttaattctt tcttagctgc
     6661 ttcttttaca gcatattcca caagcatagt tgtgcgaatt tgttgtttgt aaccttccgg
     6721 agtcaaacct gcttgtgaaa gtgcgcttga aaatgaatta ccatagcctt tagctgtctt
     6781 attataagct tctgatactt ttttatctga aactttatca ccatactgtg tatcaaaaac
     6841 acgtgagagg atcaatgtaa gcattgattg ttgtgcagct tttgatgttt ttacttgatc
     6901 ataaaaatca gagactgtaa tagtgtcgcc cttcattgta acaacatttg ttccatttga
     6961 tgttttacct gagcaggcag ctaatgtggc aactgacata agtgtcaaaa agcctgctgc
     7021 aagttttgat cttgttttca tgatttataa ctcctttttg tcattaactc cattattata
     7081 tcataatttc ttaaaaatgc cttaatcttc tagtacaata tgatctgcat ttttacgaat
     7141 catcaataaa ccatctccca aaggtaccaa agtagcagtt aaatcaggat gttgcaaagt
     7201 cgagtcaaat aaacgttgca gacctcggta gatagttcgc tgaccccttc tcacttcatc
     7261 gataggtttt gcaatatctc caccttggaa aatatcatct aaaacaacta ctcctccaac
     7321 atctaaatgc tttaatactt gaggtaaaaa aacgatatat ttagacttag cagaatccat
     7381 aaatacaaaa tcgtaagatt tatccaatgt ttgtaacaca tccacagcat cgccttccaa
     7441 aagggttatc tgattgtgat tatcatattt tgcaaagttt tccttagcca aggcaatcat
     7501 ctcttcatta cgatcgattg ttgttatctt tgcttcagga gcgttttctg ccatcaaaag
     7561 ggccgaaaaa ccaatagctg ttccgatttc taaaatgtgc ttaggttgta atgtttgcat
     7621 caaaaaacga aaataagtcg ctgtttcatg aggtataatg gggatatttt cctgtttagc
     7681 aaatgcttct aactctgcta aacagccatt attttgtttt tggtgctgac gcatatattg
     7741 aacaatttct tctttaacta ctggacggcg catattatga ttagcatttt tactgtaaga
     7801 ttgaaccact tttggctcct cttttctatt ttaaataata acttttttat tatagcaaaa
     7861 aatgtctaac tctgagaatg atttaatcca aacgagtgaa atcaagtgat attatatctt
     7921 tgagacttga aa
//
LOCUS       DATRQA010000091         7884 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.091, whole genome shotgun sequence.
ACCESSION   DATRQA010000091 DATRQA010000000
VERSION     DATRQA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.091"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(48..1244)
                     /locus_tag="VBD27_001462"
     CDS             complement(48..1244)
                     /locus_tag="VBD27_001462"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO7025466.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILEQDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRNTIAEIGYDKAEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALEVRGSLEQNPLDLIGAGDQGLMFGFAVDETPELMPLPISLAHQLVKKL
                     TDLRKSGELTYLRPDAKSQVTVEYDDNDQPIRVDAVVISTQHDPNVTNDQLHKDVIEK
                     VINEVIPSHYLDDQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKVEVQLAYAIGVAQPVSVRVDTFGTGVIA
                     EADLEAAVRQIFDLRPAGIINMLDLKRPIYRQTAAYGHMGRTDIDLPWERVDKVQALK
                     DFIASK"
     gene            1429..2364
                     /locus_tag="VBD27_001463"
     CDS             1429..2364
                     /locus_tag="VBD27_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEO7025467.1"
                     /translation="MKTYEKIYQILSKNNNFISGETMANQLNISRTAIWKGIKTLEEL
                     GLEIESVTNKGYRLVSGDILLPEQLEQEIGIKVSLNNNSASTQLDAKMGIESKLKTPH
                     LFLAPNQKKAKGRFDRPFFTSNQGGIYMSLLLQPNVPIEDIKPYTVMVASSAVKAISR
                     LTGITPEIKWVNDIYLDNKKIAGILTEAIASVESGLVTNVIIGLGINFYIKEFPRALT
                     KRAGSLFTEQPTITRNQLITEIWNLFFNIPLEDHLKVYREKSLVLDKTVSFMDGQTMY
                     SGKAIDITDKGYLVVELDDGQLKTLRSGEISLSSW"
     gene            complement(2345..2539)
                     /locus_tag="VBD27_001464"
     CDS             complement(2345..2539)
                     /locus_tag="VBD27_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEO7025468.1"
                     /translation="MTKRQFIFMALLCSFETYFFNQSVMDGSWIFAIFWGVLLLRDLQ
                     KVYAISKFTKELIKSTKKKD"
     gene            complement(2628..4292)
                     /gene="dnaX"
                     /locus_tag="VBD27_001465"
     CDS             complement(2628..4292)
                     /gene="dnaX"
                     /locus_tag="VBD27_001465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO7025469.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVSSKKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVNGEPCNHCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKLLAIRDHLAQILDKEAISYDLDALTLVARRAEGGMRDALS
                     ILDQALSLAKDNHISLDVAEEITGSISLSAIDDYVSNILAHDTTEALAKLEVIFDSGK
                     SMSRFATDLLMYLRDLLVVQAGGENSHSSDTFMANLDVKQDILFEMIDKVTSVLPEIK
                     NGSHPKVYAEMMTIQLSEMVEKNSSNIPADVTAELDSLRRELKSLKNEMSQLSRADQS
                     SSTQKVKVNNKTFTFKVDRTKILTIMEETVVDSQRSREYLEALKSAWNEILDNITAQD
                     RALLMGSEPVLANSENAILAFDAAFNAEQAMKRTDLNDIFGNIMSKAAGFSPNILAVP
                     RNDFNQIRSDFAKKMKAQKTETEPEVNHQIPEDFSYLAERIAIVED"
     gene            complement(4292..4789)
                     /locus_tag="VBD27_001466"
     CDS             complement(4292..4789)
                     /locus_tag="VBD27_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO7025470.1"
                     /translation="MNKSKKIENYQLLLLQAQALFSDETNALANLSNASAMLNAMLPN
                     SVFTGFYLFDGEELILGPFQGGVSCVHITLGKGVCGESAQTAKTLIVDDVTKHANYIS
                     CDSKAMSEIVVPMFKNGKLLGVLDLDSSLVADYDEIDQEYLEKFVGILVEHTIWNLDM
                     FGVEK"
     gene            complement(4876..5505)
                     /gene="udk"
                     /locus_tag="VBD27_001467"
     CDS             complement(4876..5505)
                     /gene="udk"
                     /locus_tag="VBD27_001467"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO7025471.1"
                     /translation="MRKKPIIIGVTGGSGGGKTSVSRAILSNFPDQKIIMIEHDSYYK
                     DQSHLTFEERVKTNYDHPLAFDTNLMIEQLNELIEGRPVDIPVYDYTKHTRSDRTIRQ
                     EPQDVIIVEGILVLEDQRLRDLMDIKLFVDTDDDIRIIRRIKRDMEERDRSLDSIIEQ
                     YTEVVKPMYHQFIEPTKRYADIVIPEGVSNIVAIDLINTKVASILNEAK"
     gene            5650..6732
                     /locus_tag="VBD27_001468"
     CDS             5650..6732
                     /locus_tag="VBD27_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7025472.1"
                     /translation="MITKFPDQWQDKLTQRQFDDLTDIQNKLFQPITDGDNILGISPT
                     GTGKTLAYLFPTLLKLQPKKSQQLLILAPNSELAGQIFDVTKEWAEPLGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTAGRVFELVKLKKIKMMNINTIVLDEFDELLGDSQYHFV
                     DNIINRVPRDQQMIYISATNKLDNSKLADNTITIDLSDQKLDTIKHYYITVDKRERTD
                     LLRKFSNIPDFRGLVFFNSLSDLGACEERLQFNRASAVSLASDINIKFRKVILEKFKN
                     HDISLLLGTDLVARGIDIDNLEYVINFDIARDKETYTHRSGRTGRMGKEGCVITFVTH
                     KEELKQLKKYATVAELVLHNQKLHLK"
     gene            complement(6765..>7884)
                     /locus_tag="VBD27_001469"
     CDS             complement(6765..>7884)
                     /locus_tag="VBD27_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO7025473.1"
                     /translation="KTVEVVKRQKTERVLFLEPKGYDKSLSADILKWNQKSFEHKKFY
                     DNQYIILRPQLADSNFANVKKLSIYQILYQKEKGSMFQKSSRLLRTYLLDQNKKPFEL
                     DELLAHNISGFKAILENIAPGTQLKEHDSNKEFLKTGRVTDGLDVKDGKLIINDLKLP
                     LDKLYNVIDESYLKSSDLDLVSNLKAKAPRVALTFDDGPNEKTTPKALEILKRYNAKA
                     TFFIMGQSAVGHTDILQRMHAEGHEIGNHTWDHPNLTKLPAEKIKEEIHKTNDLIMKA
                     TGQKPVYLRPPYGATNATVKTVTGLKEMLWSVDTEDWKNHNTQAMMTNIKKQLRPGGV
                     ILMHDIHQTSIDALPTIMDYLTTQGYYFVTVGELYSTP"
BASE COUNT         2571 a         1488 c         1245 g         2580 t
ORIGIN      
        1 aaattatcat gagcaaaaaa agcgtaaact ttcgtttacg ctttttatta tttgctagca
       61 ataaaatcct ttaaggcctg gactttatca actcgctccc atggcaaatc gatatcagta
      121 cgtcccatat gcccataggc tgctgtttgt cgataaatag gtcgtttgag gtccaacata
      181 ttaataatgc ctgctggtcg caaatcaaat atttggcgta cagctgcctc taggtctgct
      241 tctgctataa cacctgttcc aaatgtatct acacgcacag agacaggctg agcaacacca
      301 attgcataag caagttgtac ttctactttt ttagctaaat cagctgcaac aatatttttt
      361 gcaatataac gagctgcata agatgctgaa cgatctactt tagtagcatc tttacctgaa
      421 aatgcaccac caccatgacg agaataacca ccataagtat ctacaataat cttacgacct
      481 gttagtcctg aatctccttg agggcctcca ataacaaagc gccctgtagg attgataaag
      541 aattttgttt ggtcatctaa ataatggctt ggaataactt cattgataac tttctcaatc
      601 acgtccttat ggagttggtc attagttaca ttaggatcat gctgtgtcga aataactact
      661 gcatccacac gaattggttg atcattatca tcatattcaa ccgttacttg agattttgca
      721 tctggtcgaa gataggtgag ttccccagac ttacgtagat ctgtcaattt cttaactaac
      781 tgatgagcta aagaaattgg caatggcatt aactcaggtg tttcatcaac agcaaaccca
      841 aacatcaaac cttggtcacc tgcaccaatt aaatctaaag gattttgttc aagagaacca
      901 cgtacctcaa gagcttcatt gactccctgt gcaatgtctg gcgactgttc aacgagtgac
      961 ggatgcactc cgacagattc agctgagaag ccatattcag ctttatcata gccaatttca
     1021 gcgattgtgt tacggacaac tcggttaata tcaacatagg ctgtcgttga tatctcgcca
     1081 aatacatgaa ccgaacctgt ataaacagct gtttcggctg ctacgtgggc atctggatct
     1141 tgctctaaaa ttgcatctaa aatcgcatcg gaaatttggt cagcaatctt atcgggatgt
     1201 ccctcagata ctgactcaga cgtgaaaagt ttacgttctg acataaaaat gtccccccta
     1261 aaatgtaaaa tgtgtcgagt tacaaagaac tagcctctag gctacctttt cgggactcat
     1321 atagctattg taccactatt tcatcttttg tgaaataagt taccaataaa taaaaaatta
     1381 tctctatttt ctttagtctt ttttctcgat ttatactata ctagatttat gaaaacctac
     1441 gaaaaaattt atcaaatact atcaaaaaat aacaacttta ttagcggaga aactatggca
     1501 aatcaactta atatttcccg cacagctatt tggaaaggta taaaaacgtt agaagaatta
     1561 ggcctagaga ttgagtctgt cactaacaag ggctaccgat tggtatcagg tgacatatta
     1621 ttaccagaac aactcgaaca agaaattggt attaaggtta gtcttaataa taatagtgct
     1681 tcaacccaac tagatgctaa aatggggata gaaagcaaat taaagactcc acaccttttt
     1741 ttagctccta atcaaaaaaa agctaaagga cgttttgata gacctttctt tacatcaaat
     1801 caaggcggca tctatatgtc tcttttatta caacctaatg taccgattga agatataaaa
     1861 ccttatactg tcatggtagc ctctagtgct gtaaaagcta tctcacggtt aacaggaatt
     1921 actcctgaaa ttaagtgggt caatgatatt tatttagata ataaaaaaat agctggtatt
     1981 ttaaccgagg ctatcgcttc tgttgagagt ggattggtaa cgaatgttat tataggatta
     2041 ggtatcaatt tctatatcaa agagttccct agggcgttaa ctaagcgcgc aggtagtctc
     2101 tttacagagc aaccgaccat aactagaaat caattgatta cagaaatctg gaatctcttt
     2161 ttcaatattc cactagagga tcacttaaag gtatatagag aaaaatccct agtcttagat
     2221 aaaaccgtta gctttatgga tggtcaaaca atgtattctg ggaaagctat tgatattact
     2281 gataaaggat atttagtggt tgagttagat gatggtcagt tgaaaacact tcgcagtggc
     2341 gaaatcagtc tttcttcttg gtagatttga taagctcttt tgtgaatttt gaaatcgcat
     2401 acaccttttg aagatctcga agtaataaaa ctccccaaaa aatagcaaaa atccaggagc
     2461 catccataac ggattgattg aaaaaataag tttcaaaaga gcataaaagc gccataaaaa
     2521 taaattgtcg ttttgtcata tcaccattat aacagaatct actaaataat taaaaaactt
     2581 atcaaaaaaa tagttgcctg ttacgggcaa ctatttttag ttacgtttta atcttcaacg
     2641 attgctattc gttctgctag ataagaaaag tcttctggaa tctgatgatt aacttcaggt
     2701 tctgtttccg tcttttgagc tttcattttc tttgcaaaat cgctccgaat ttgattaaaa
     2761 tcatttctag gtacagctaa aatattaggt gaaaaacctg ctgctttact cataatatta
     2821 ccaaatatat cattgagatc ggtacgtttc atagcttgtt cagcattaaa ggccgcatca
     2881 aatgccaaaa tagcattctc gctattagct aagacaggtt cagatcccat taataaggct
     2941 ctatcttgag ccgtaatatt atctaatatc tcgttccaag cactctttaa agcttctaaa
     3001 tattcacgtg aacgttggct atctacaacc gtttcttcca taatggtcaa aatctttgta
     3061 cggtcaactt tgaatgtgaa tgtcttatta ttaaccttta ctttttgagt tgatgaactt
     3121 tgatctgctc gagagagttg gctcatttcg ttttttaacg attttaactc tcgacgaaga
     3181 ctatctaatt ctgctgtcac atctgcgggt atgtttgacg aatttttttc taccatttca
     3241 gacaattgaa tggtcatcat ctctgcataa actttaggat ggctaccatt ttttatctca
     3301 ggaagaacag atgtcacttt atcaatcatt tcaaataaaa tatcttgttt aacatcaaga
     3361 ttggccataa aagtatcact tgaatgacta ttttcaccac ctgcttgaac aacaagtaaa
     3421 tctcttaagt acatcaataa atctgttgca aaacgactca tacttttgcc actatcaaag
     3481 attacttcaa gtttagcgag tgcttcagtt gtatcgtgag ccaatatatt cgatacataa
     3541 tcatctatag cagataagga aatgctccct gtaatttctt ctgctacatc taaagaaatg
     3601 tggttatctt tcgctaaact caaggcttga tcgagaattg ataaagcatc acgcatacca
     3661 ccttcggctc gtctagcgac tagtgtcaga gcatccaaat catatgaaat agcttcttta
     3721 tctaaaatct gtgctaaatg atctcgaata gctaataatt taatcgcttt aaattcaaag
     3781 cgctggacac gagataagat tgttgcaggg attttatgga gttctgttgt tgctaatata
     3841 aaaacaacat tttctgttgg ctcttcaagt gttttcaata gtgcattgaa ggcacctgta
     3901 gacaacatat gaacttcatc aataatataa actttatagg ttgccctact tggagcataa
     3961 gttgatttgt ccctgatatc acgaatttca tcgacaccat tatttgacgc agcatcaatt
     4021 tcaatgacat cctctaaact accatttgta atatctcgac aaatatcaca atgattacaa
     4081 ggttcgccat taacttgatt tgggcaattc atcgcttttg caaatatttt agcagcactt
     4141 gtttttccag ttcccctagg tcctgaaaaa aggtaagcat ggctaatttt tttgctggac
     4201 acagcttgct ttaaagtagt tgaaataaca ctttgtccaa ccatttcatc aaatgtttgg
     4261 ctacgatatt tccgataaag ggcctgatac attacttttc aactccaaac atatccaaat
     4321 tccaaatcgt atgttctact agaataccta caaatttttc taagtattct tgatcaatct
     4381 catcataatc tgctactaaa gaagaatcta aatctagaac tcctagaagt ttgccatttt
     4441 taaacatggg tactacgatt tcactcatag cttttgaatc acaggagata tagttagcat
     4501 gctttgtaac atcatcaaca atcagcgtct tagcagtttg tgcagattca ccacaaacac
     4561 cttttcctaa agtaatatgc acacatgata caccaccctg aaaagggcca agaattaact
     4621 cctctccatc aaataaataa aagcctgtaa atacagaatt tggaagcata gcatttagca
     4681 tagctgaagc atttgataag ttggcaagag catttgtttc atctgagaat agagcttgcg
     4741 cttgtagtaa taataattga taattttcga ttttcttaga cttgttcata gcacttatta
     4801 tagcataatt tgaaagttac taaaactaaa taaccatcct atataaaaca aaaaaataaa
     4861 cacaaaccat tgtgtttatt ttgcttcatt taagatgcta gccactttgg tattgattaa
     4921 atcaatagcg acaatattac ttactccttc tggaattaca atatccgcat aacgctttgt
     4981 tggttcgata aattgatgat acataggttt tacaacttct gtatactgtt caataatact
     5041 atctaaactt ctatcgcgct cttccatatc acgcttaata cgacgaataa tacgaatatc
     5101 atcatctgta tcaacaaata atttgatatc cattaaatca cgcaaacgct gatcttctaa
     5161 taccaaaatt ccctcaacaa taatgacatc ctgaggttct tggcggatag ttctatcact
     5221 acgggtatgt tttgtgtaat cataaaccgg aatatcgacc ggacgcccct caatcaattc
     5281 gttcaattgc tcaatcatca aattggtatc aaaagcaagg ggatgatcat aattggtttt
     5341 tacacgttct tcaaaagtca aatgactttg atctttatag taagaatcat gttcaatcat
     5401 gataattttt tgatctggga aatttgataa aatcgcccga gaaacactgg ttttaccacc
     5461 accagatcca cctgtaacac caataataat aggtttttta cgcatttgtc actccaaatt
     5521 aaatttatcc tctatctatt gtactataaa cttatttttt ttaaagtcta aattttattg
     5581 aaaacgctct aaaaataaaa agcatgttat aataacaatg acaacatata aaataacaag
     5641 aggattctta tgataacaaa atttccagac caatggcagg ataaattaac ccagcgtcaa
     5701 tttgatgatc taacagatat tcaaaataaa ctttttcaac ctattactga tggtgataat
     5761 atccttggaa ttagccctac aggtactggt aaaactctag catacctttt cccaacgctt
     5821 cttaagttgc aacctaaaaa gagccaacaa ctcttaattt tagctccaaa ttcagaatta
     5881 gctggacaaa tcttcgacgt tacaaaagaa tgggctgaac cattaggact gacagcacaa
     5941 ctattccttt cagggtctag tcaaaaacgc caaattgagc gactaaaaaa aggaccagaa
     6001 atccttatag gaactgctgg acgcgtattt gaacttgtga aactaaaaaa aataaaaatg
     6061 atgaacatta atactatcgt ccttgatgag ttcgatgaat tactcggcga ctcacaatat
     6121 cattttgtgg acaatattat taatcgcgtt cctcgtgatc agcaaatgat ttacattagt
     6181 gccaccaaca aactggacaa tagtaaactt gccgataaca ctataacaat tgacctatct
     6241 gatcaaaaac ttgatactat aaaacattat tatattacag ttgataaaag agagcgtact
     6301 gacttacttc gtaaattttc aaatattcct gattttagag gtcttgtctt ttttaacagc
     6361 ttatcagact taggtgcatg tgaagaacgt ttacaattta atagggcctc ggcagtttct
     6421 ttagcaagcg atattaatat taaattccgt aaagttatct tagagaaatt taaaaaccat
     6481 gatatttcat tactcctagg aactgatttg gtagctcgtg gcattgatat cgacaaccta
     6541 gaatatgtga ttaattttga tatcgcacgc gacaaagaaa catatacaca tcgttctgga
     6601 cgaactggtc gtatggggaa agagggatgt gttattacat ttgtaacaca taaagaagag
     6661 ctcaaacaac ttaaaaaata cgccaccgtt gcagaacttg tacttcacaa tcaaaaatta
     6721 cacttaaaat aaaaaaagaa acaagtaact tgtttctttt tttattaagg cgttgaatat
     6781 aattcaccta cagtaacaaa ataataaccc tgggttgtca gataatccat aattgtaggc
     6841 aaagcatcta tggatgtctg gtgaatatca tgcatcaaaa tcactccccc aggacgtaat
     6901 tgttttttga tattggtcat cattgcttgt gtattatgat ttttccagtc ttctgtatca
     6961 actgaccaca acatttcttt caatcctgtc actgttttta cagtagcatt tgttgctccg
     7021 taagggggac gtaagtacac tggtttttgt cctgttgctt tcatgattag atcattagtc
     7081 ttatgaatct cttccttgat tttctccgct ggtagtttgg ttaaattagg atgatcccat
     7141 gtatgattac caatctcatg accttcagca tgcatccttt gtaaaatatc tgtgtgccct
     7201 acagcacttt gtcccattat aaagaaagtc gctttagcat tatatctttt aaggatttct
     7261 aaggctttag gagtcgtttt ttcatttggt ccatcatcaa aggtaagtgc aacacgtggt
     7321 gctttagctt ttaagttaga aactaaatct agatcacttg attttaaata tgactcatcg
     7381 atcacattat ataatttatc aagtggtaat tttaaatcat taataattaa cttaccatct
     7441 ttaacatcta gcccatctgt tactcttcca gtttttaaaa attctttatt gctatcatgt
     7501 tcctttagtt gagttcctgg agctatattt tctaaaatag ccttaaatcc agatatatta
     7561 tgagctaaaa gctcatctaa ttcaaaaggt ttcttatttt gatcaagtaa gtaggttctc
     7621 aaaaggcgac ttgatttttg aaacatactt cctttttctt tttgatataa gatttgataa
     7681 atggataatt ttttaacatt tgcaaagtta gaatctgcta attgaggacg tagaataatg
     7741 tattgattgt cataaaattt tttatgctca aaactttttt gattccactt taaaatatca
     7801 gcgcttagac ttttgtcata tcctttaggt tcaagaaaaa gaacacgttc agttttctgt
     7861 cttttcacca cttctaccgt tttc
//
LOCUS       DATRQA010000092         7854 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.092, whole genome shotgun sequence.
ACCESSION   DATRQA010000092 DATRQA010000000
VERSION     DATRQA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.092"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            62..1870
                     /gene="dnaG"
                     /locus_tag="VBD27_001470"
     CDS             62..1870
                     /gene="dnaG"
                     /locus_tag="VBD27_001470"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO7025474.1"
                     /translation="MGYFCGGHDLAIDKEKISEIKNSVNIVDVIGEVVGLTKTGRNHL
                     GLCPFHKEKTPSFNVIEDRQFFHCFGCGRSGDVFKFVEDYQHISFLDSVQVLAERSGI
                     PLDTNFKGQVPKKPKANQSLLDIHRVASGFYHAYLMTTNDGERARQYLAERGVTEDLI
                     KHFQIGLSPGGQDFLYRRLAKEFDEKTLMSSGLFNYSENSNQFYDSFNNRIMFPLTND
                     IGEVIAFSGRVWTQEDIDRKQAKYKNSRATPIFNKSYELYHLDKARAVINKAHEVYLM
                     EGFMDVIAAYRAGIENVVASMGTALTNEHVRHLKRFTKKVVLTYDGDRAGQNAIDKSL
                     ELLSDMTVDIVRIPNKMDPDEFLQANSAEDFKQLLENGRISNTEFYIHYLKPENTDNL
                     QSEIAYVEKIAKLIAKSPSITAQNSYITKVAELLPDFDYFQVEQSVNNERLHHRSQQQ
                     ASSSVQTSATVQLPQTGKLSAITKTEMQLFHRLLNHPYLLNEFRNRDNFYFDTTEIQV
                     LYELLKESGEITSYDLSQESDKVNRTYYIILEEQLPVEVSIGEIEAVEKARDRLLKER
                     DLRKQSQLIRQSSNQGDEEGALAALENLIAQKKNME"
     gene            1878..2987
                     /gene="rpoD"
                     /locus_tag="VBD27_001471"
     CDS             1878..2987
                     /gene="rpoD"
                     /locus_tag="VBD27_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO7025475.1"
                     /translation="MAEKKGNTTFNVQVADFIRNHKKQGTAIDDEVTEKLVIPFVLDA
                     DQIDDLLERLTDGGISITDKEGNPSTKYVVEGPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELAVAVAEGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLKDFMED"
     gene            3096..3437
                     /locus_tag="VBD27_001472"
     CDS             3096..3437
                     /locus_tag="VBD27_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEO7025476.1"
                     /translation="MSEVKQYSEEEVGKIKDRILEALEMVIDPELGIDIVNLGLIYEI
                     RFEDNGRTEIDMTLTTMGCPLADLLTDQIHDVMKTVPEVTETEVKLVWYPAWSVDKMS
                     RYARIALGIRS"
     gene            3527..4381
                     /gene="rfbD"
                     /locus_tag="VBD27_001473"
     CDS             3527..4381
                     /gene="rfbD"
                     /locus_tag="VBD27_001473"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO7025477.1"
                     /translation="MILITGANGQLGSELRHLLDERTQEYVAVDVAEMDITNAEMVDK
                     VFEEVKPSLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKAAAKHDATLVYISTD
                     YVFDGEKPVGQEWEVDDLPDPKTEYGRTKRMGEELVEKYTSKFYTIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQHGRPTWTRTLAEFMTYLAENQKDFGYYHLSNDAKEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLEKAKATGFVIPTWQDALKE
                     FYKQEVKK"
     gene            4498..5652
                     /locus_tag="VBD27_001474"
     CDS             4498..5652
                     /locus_tag="VBD27_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEO7025478.1"
                     /translation="MQDVFIIGSRGLPARYGGFETFVSELINHQKSSDIKYHVACLSD
                     KEHHTHFNFADADCFTINPPQLGPARVIAYDIMAINYALDLVKTHDLKEPIFYILGNT
                     IGAFIWHFANKIHKVGGLLYVNPDGLEWKRSKWSRPTQRYLKYAEKCMTKNADLIISD
                     NIGIENYIQSTYSNVKTRFIAYGTEINSRKLSSDDPRVKQLFKKWNIKSKGYYLIVGR
                     FVPENNYETAIREFMASDTKRDLVIICNHQNNPYFEKLSLKTNLQQDKRVKFVGTLYE
                     KDLLDYVRQQAFAYIHGHEVGGTNPGLLEALANTDLNLVLDVDFNKSVAGLSSFYWTK
                     KEGDLAKLINDSDQQQDLSTYGDRAKAIIQENYTWKKIVEEYEDLFLNES"
     gene            5642..6583
                     /locus_tag="VBD27_001475"
     CDS             5642..6583
                     /locus_tag="VBD27_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7025479.1"
                     /translation="MKVNILMATYNGEKFLAQQIESIQKQTFKEWNLLIRDDGSSDKT
                     CDIIRNFTAKDSRIRFINENEHHNLGVIKSFFTLVNYEVADFYFFSDQDDVWLPEKLS
                     VSLEAAKHKASDVPLLVYTDLKVVNQELNILQDSMIRAQSHHANTTLLPELTENTVTG
                     GTMMINHALAEKWFTPNDILMHDWFLALLAASLGEIIYLDLPTQLYRQHDNNVLGART
                     MDKRFKILREGPKSIFTRYWKLIHDSQKQASLIVDKYGDIMTANDLELIKCFIKIDKQ
                     PFMTRLRWLWKYGYSKNQFKHQVVFKWLIATNYYNKR"
     gene            6585..7316
                     /locus_tag="VBD27_001476"
     CDS             6585..7316
                     /locus_tag="VBD27_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7025480.1"
                     /translation="MMKKILLVIPAYNEEGSIAKTVQTIVDFKASRSLPFELDYIVIN
                     DGSTDGTPELLDRLGLNHIDLVQNLGIGGCVQTGYLYANRNHYDVAVQFDGDGQHDIR
                     SIEDVVMPILNDEADFVIGSRFVDKKHQNFQSTAMRRLGINLISAAIKLTTGHKVYDT
                     TSGYRAANAALIAYLSCHYPVQYPEPESTARILKKGYRLKEVTANMFEREAGTSSISS
                     LKSIFYMTDVLTSIIIAGFIKEDDK"
     gene            7313..7666
                     /locus_tag="VBD27_001477"
     CDS             7313..7666
                     /locus_tag="VBD27_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEO7025481.1"
                     /translation="MSLALQIEMFILAIVLLYMIIRMVNKNTFSVKRSLPWLLVGLIL
                     VFISVFPGVVSFIAHHLGFALTMNFLLFGAILFLFCLELFDTSSNTKRDEQIKRLIQE
                     VSLLKKEIEDSTLDE"
     gene            7659..>7854
                     /locus_tag="VBD27_001478"
     CDS             7659..>7854
                     /locus_tag="VBD27_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface protein"
                     /protein_id="HEO7025482.1"
                     /translation="MSKKHIFICLFFMLFLVGCQNNKKVKTDKKVDITAYYYHNNTPQ
                     YSVQINAIHLQTALDKVRTGE"
BASE COUNT         2646 a         1167 c         1581 g         2460 t
ORIGIN      
        1 aaagcttttg aaaaaattta cacagaaaag aaaaaatgat ataatcaaat gtaaaactat
       61 aatgggttat ttctgtggag gtcatgactt ggcaattgat aaagaaaaaa tttctgaaat
      121 caagaacagt gttaacattg ttgatgtcat cggtgaagtt gtcggtttga caaaaacagg
      181 acgcaatcat cttggtttat gcccatttca taaagaaaaa acgccatctt ttaatgttat
      241 cgaagatagg cagttttttc attgttttgg ttgtggtcgc tctggtgatg tctttaaatt
      301 tgtggaagat taccaacata ttagtttttt agatagtgta caagttctag ctgaacgttc
      361 aggaatacct ctagatacta attttaaggg gcaagttcct aaaaaaccta aagctaatca
      421 gtcgctttta gatattcata gagttgcttc gggattttac catgcctatt taatgacaac
      481 taatgatggt gaacgcgcta gacaatacct tgccgagcgc ggagtgacag aggacttaat
      541 taaacatttt caaataggat tatcgccggg agggcaggat tttctatatc gtcgtctggc
      601 aaaagagttt gatgaaaaaa ctttgatgtc ttctggtttg ttcaattatt cagaaaatag
      661 caatcagttt tacgattcat ttaataatag aattatgttt ccgttaacta atgatattgg
      721 tgaagtcatt gcgttttcag gacgagtttg gactcaagag gacattgata gaaaacaagc
      781 taaatataaa aattctcgtg ccacccctat ttttaataag tcttacgagc tttaccattt
      841 agataaagcg cgtgctgtaa ttaataaagc acacgaagtt tacctaatgg aaggctttat
      901 ggatgttatt gcagcttatc gtgcaggaat tgagaatgtt gttgcttcta tggggaccgc
      961 tttaaccaat gaacacgtta gacaccttaa aagatttact aagaaagttg ttctgactta
     1021 tgatggtgat agagctggtc aaaacgcaat tgacaagtct ttagaactgc tatcagatat
     1081 gactgttgat atcgttagga tacctaataa aatggaccct gatgaatttc tacaagcaaa
     1141 ttcagcagaa gattttaaac aactcttaga aaatgggcgc atcagtaata cagagtttta
     1201 tatccactac ttaaagcctg aaaacaccga taatttacaa tctgaaattg cttatgttga
     1261 aaaaatagcg aaattaattg ctaaatctcc atcgataaca gcacaaaatt catatattac
     1321 aaaagtggct gaactcttgc cagattttga ttattttcaa gtagaacaat cggttaataa
     1381 cgaacgattg catcatagaa gtcaacagca agcttcatct tcagtacaaa caagtgctac
     1441 agtgcaatta ccacagacag gtaagttatc agctatcact aaaacagaaa tgcaattgtt
     1501 tcatcgatta ttgaatcatc cctacctgtt aaatgaattc cgtaataggg ataattttta
     1561 ttttgataca acagaaatac aagttttata tgagttgcta aaagaaagtg gtgagatcac
     1621 gtcttatgat ttatcacaag aaagtgacaa agttaatcgt acttattata taattttaga
     1681 ggaacaatta cctgtagaag tatctattgg agagattgaa gccgtcgaaa aggcacgtga
     1741 ccgtttattg aaagaacgtg atttaagaaa acaaagccag ttaattagac aatctagtaa
     1801 tcaaggtgat gaagaaggag ccttggctgc attagaaaat ttaattgctc aaaaaaaaaa
     1861 tatggaatga ggaacctatg gcagagaaaa aaggaaatac aacttttaat gttcaagttg
     1921 cggattttat tcgcaatcat aaaaaacaag gtactgcaat tgatgatgag gtaacagaaa
     1981 aacttgttat tccttttgtt ttagatgcag atcaaattga tgatttgtta gaacgattga
     2041 cagacggtgg catttcaatc acagataaag aaggcaatcc ttcaacaaag tatgtggtag
     2101 aagggccaaa acctgaagaa ttgactgatg aagagctcat aggaagtaat tctgctaaag
     2161 tcaatgaccc agtacgtatg tacttgaagg aaattggtgt tgttcctctt ttaacgaatg
     2221 aagaagaaaa agaattggct gttgcggttg ctgagggaga tttaatggct aaacaacgtt
     2281 tagctgaagc aaaccttcgt cttgttgtct caattgcaaa acgttatgtt ggacgtggaa
     2341 tgcaatttct tgatttaatt caagagggta acatggggct catgaaagct gttgataaat
     2401 ttgattattc aaagggattc aaattttcaa cttatgcaac atggtggatt cgtcaagcta
     2461 taacacgtgc aattgcagat caagcacgta cgattcgtat tcctgttcat atggttgaaa
     2521 cgattaataa attggtccgt gaacaacgta atcttcttca agaacttggt caagatccaa
     2581 ctcctgagca aatcgcagaa cgcatggata tgacaccaga taaagtacgt gaaatcctaa
     2641 aaattgctca agagccggta tcgctagaaa cgccaatcgg tgaggaagat gatagccatt
     2701 tgggtgattt cattgaagac gaagttattg aaaatccagt agattatact acacgtgttg
     2761 ttcttcgtga gcaattagat gaggttttag atactcttac tgaccgtgaa gagaatgttc
     2821 ttcgtttacg ctttggtctt gacgatggta aaatgcgtac gttagaggac gtaggtaaag
     2881 tatttaatgt tactcgtgag cgcattcgtc agattgaagc taaagccctt agaaaacttc
     2941 gccatcctag tcgtagcaag caacttaaag atttcatgga agattgattg cttctcgata
     3001 atcgttttta gaataggaag tttagtttct aataaaaatg atttgcaaag gagttatctt
     3061 aaagacggta ttaatatttt cagagaggta ttataatgtc agaagtaaag caatatagcg
     3121 aagaagaagt tggaaaaata aaagatcgta tcctagaagc tttggagatg gttatcgatc
     3181 ctgaattggg aatagatatt gttaaccttg ggttaattta tgagattcgc tttgaagata
     3241 acggtcgaac tgaaattgat atgactttaa cgactatggg atgcccgtta gcagatttat
     3301 tgacagatca aattcatgat gttatgaaaa cagtcccaga ggtaactgaa accgaggtga
     3361 aattggtatg gtacccagcc tggtctgttg ataagatgag tcgttatgct cgtatcgctt
     3421 tgggaattcg ttcataagat aaagagtaaa gggatgaaat attccctttt ttagtgtaca
     3481 ataaacgaag aattaaaagt taaacttatt tttaaaggag caaggaatga ttttaattac
     3541 aggggcaaac gggcaattag gtagtgaatt acgtcacctt ttagacgaac gaactcaaga
     3601 gtatgttgct gttgatgtag ctgaaatgga tattacaaat gccgagatgg tggacaaagt
     3661 ctttgaagag gtgaagccat ctttagttta ccattgtgca gcttacacag ctgttgatgc
     3721 ggcagaagat gaaggaaaag agctagactt tgcaatcaat gtcactggta cagagaatgt
     3781 cgctaaggct gctgctaaac atgatgcaac acttgtttac atttcgacgg actacgtgtt
     3841 tgatggtgaa aaaccagtgg ggcaagaatg ggaagtagat gatctgcctg atcccaaaac
     3901 agagtacggt cgtacaaaac gtatgggtga agagttagtt gaaaaatata cttcaaaatt
     3961 ctataccatt cgcacagcgt gggtatttgg taactatggt aaaaactttg tctttacgat
     4021 gcaaaacctt gcaaaaacac acaaaactct aactgttgtt aacgaccaac atggacgacc
     4081 aacatggaca cgtactttgg ctgagtttat gacttattta gctgaaaatc aaaaagattt
     4141 tggttactat catttatcaa atgatgctaa agaagataca acatggtatg attttgcggt
     4201 tgaaatctta aaagacacag atgtggaggt taagcctgtt gattctagtc aattcccagc
     4261 aaaagcaaaa cgtcctttaa attcaactat gagtttggaa aaagcgaaag caacaggatt
     4321 tgtgattcct acttggcaag atgctttaaa agagttctac aagcaggaag ttaaaaaata
     4381 agatcaaaac aaagagtagt aaggctagag aatcctttta gcttactgct ctttttagct
     4441 aacttttcta atttatggta taattgtatg gattgtttag ctagaatgga gaagatgatg
     4501 caagatgttt tcattatagg aagtagaggg ttgccagctc gttacggtgg ttttgaaact
     4561 tttgtttcag aattgattaa tcatcaaaaa agttccgaca taaaatacca tgttgcatgc
     4621 cttagtgata aagaacatca tactcatttt aactttgctg acgctgattg ttttactata
     4681 aatcctcccc aattagggcc agcacgtgtg attgcttatg atattatggc cattaattat
     4741 gcccttgact tggttaagac acatgattta aaagagccta ttttttatat tttaggaaat
     4801 acaattggtg cctttatttg gcattttgcc aataaaatac ataaagtcgg tggcttattg
     4861 tatgttaatc cggatggttt agagtggaag cgatcaaagt ggtctcgtcc cacacagcgt
     4921 tatttaaaat acgccgaaaa atgtatgact aaaaatgcag acctaattat ttctgataat
     4981 attggtattg aaaattacat tcaatctacc tactctaatg tgaagacaag gttcattgct
     5041 tacggtacag agattaattc taggaaatta tcgtcagatg atccacgtgt caaacagttg
     5101 tttaaaaaat ggaatattaa gtctaagggt tactatctaa tcgttggtcg atttgtccct
     5161 gaaaacaatt atgaaacggc tattagggag ttcatggctt cagatactaa gcgtgattta
     5221 gttattatct gtaaccatca aaataacccc tactttgaaa agttgtcctt aaagacaaac
     5281 cttcaacaag ataaaagagt taagtttgta ggtacgctct atgaaaaaga tctgctggat
     5341 tatgttcgtc aacaagcctt tgcttatatt catgggcatg aagttggcgg tactaatcca
     5401 ggactgcttg aggctttagc taatactgat ttgaatcttg ttctagatgt tgatttcaac
     5461 aaatcagtag caggtctctc aagtttttac tggactaaaa aagaggggga tttagctaag
     5521 cttattaacg atagtgatca acaacaggat ttatctactt acggtgatcg agcaaaagcc
     5581 attattcaag aaaactatac ttggaaaaaa attgttgaag aatatgagga tttgttttta
     5641 aatgaaagtt aatattttaa tggccaccta caatggagag aagtttctgg cgcagcaaat
     5701 cgaaagtatc caaaagcaaa cctttaaaga gtggaacctc ctaatcagag atgatggttc
     5761 aagtgacaag acatgtgata ttattagaaa ctttactgct aaagattctc gaattcgttt
     5821 cattaatgaa aatgagcacc ataatttagg agttattaaa tcatttttta cgctagttaa
     5881 ttacgaagtt gcagatttct atttttttag tgatcaagat gatgtatggt tgccagaaaa
     5941 gttatctgta agtttagagg cagcaaagca taaagcatct gatgtaccat tacttgttta
     6001 tactgatttg aaagttgtta atcaagaatt aaatatttta caagacagca tgattcgggc
     6061 tcaatcgcac catgccaata cgacattatt accagaacta actgagaata ctgttactgg
     6121 tgggactatg atgataaatc atgcattagc tgagaagtgg tttactccta atgatatctt
     6181 aatgcatgat tggtttttag cactcttagc agcaagttta ggagaaatca tttatttgga
     6241 tttaccaact caactatacc gtcaacatga caataatgtt ttgggggcga ggacaatgga
     6301 taagagattt aagatacttc gtgaaggtcc taaatctata tttacaaggt attggaaact
     6361 tatccatgac tcacaaaagc aagcttcctt aattgttgat aagtatggtg atattatgac
     6421 agctaatgat cttgagctca ttaaatgttt tataaaaatt gacaaacagc cttttatgac
     6481 aagattaagg tggttatgga aatatggtta tagcaaaaat caatttaagc atcaagttgt
     6541 gtttaaatgg ttgattgcaa caaattatta caataagaga taagatgatg aaaaaaattt
     6601 tactagtgat acctgcttat aatgaagaag gtagtattgc aaagacagtc cagactattg
     6661 tagattttaa ggcgtctcgc tcactacctt ttgagctgga ttatattgtt attaacgatg
     6721 ggtcaaccga tggtactcca gagttattag atagattggg tttaaatcat attgaccttg
     6781 ttcaaaatct aggtattggt ggctgtgtgc agacaggata cttatacgct aatcgtaatc
     6841 attacgatgt tgccgttcaa tttgatggtg atggtcaaca tgatattaga agcattgaag
     6901 atgttgttat gccaatctta aatgacgaag cagattttgt tattggttct cgctttgttg
     6961 ataaaaaaca tcaaaatttt caatcgacag caatgaggcg tcttggcata aatttaatat
     7021 ctgctgctat aaaattaacg acgggacaca aagtctatga tacaacaagt ggttataggg
     7081 cagccaacgc ggcgctaata gcttatttat cgtgtcatta tccagtacaa tacccagagc
     7141 ctgaatcaac tgctcggatt ttgaaaaaag gctatcgttt gaaggaagtc acagctaata
     7201 tgtttgaacg tgaggcaggt acttcaagta taagttccct aaaatcgatt ttttatatga
     7261 cggatgtttt aacatcaatt attattgcag ggtttattaa ggaggatgat aaatgagttt
     7321 agcattgcaa attgaaatgt ttattttagc cattgtccta ctttatatga ttataagaat
     7381 ggttaataaa aacacatttt ctgttaaacg ttctttacct tggctattag ttggtttaat
     7441 tctagtcttt attagtgtat ttcctggggt ggtctcattt atcgctcatc accttggatt
     7501 cgctttaacg atgaatttct tgttatttgg tgctatttta tttctgtttt gtttagaatt
     7561 atttgatact agtagcaata ccaaaagaga tgagcaaatt aaacgcctga ttcaagaggt
     7621 ttctttgttg aaaaaagaga tagaggatag cactttagat gagtaaaaaa catattttta
     7681 tttgtctgtt tttcatgctt tttttagtag gttgccaaaa taataaaaaa gtaaaaacag
     7741 ataaaaaagt agatattaca gcttattatt atcataataa taccccgcaa tacagtgttc
     7801 agattaatgc tattcattta caaacagcat tggataaagt gaggacgggg gagt
//
LOCUS       DATRQA010000093         7759 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.093, whole genome shotgun sequence.
ACCESSION   DATRQA010000093 DATRQA010000000
VERSION     DATRQA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.093"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            18..491
                     /locus_tag="VBD27_001479"
     CDS             18..491
                     /locus_tag="VBD27_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEO7025483.1"
                     /translation="MSKKNKIKKTLVDQILDKAKIEHDSLQLDALQGDLPNGIQKQDI
                     FKTLALIGDKTGPIIGILPLTEHLSEKKLAKISGNKKVQMIPQKDLQKITGYIHGANN
                     PIGIRQKHNYPIFIDTIALEKQELIVSAGEIGRSIRINSEVLADFVNAKFADIKE"
     gene            513..1154
                     /locus_tag="VBD27_001480"
     CDS             513..1154
                     /locus_tag="VBD27_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO7025484.1"
                     /translation="MKFYLVRHGKTQWNLEGRFQGANGDSPLLEEAIEELEELGQYLS
                     SIHFDAVYSSDLGRARDTVNILNDANSCPKEIHYTSQLREWALGTLEGCKIATMQAIY
                     PRQMTAFYQNPLQFKHDMFGAESLYQTTHRVESFLRSLASKNYDKVLIVGHGANLTAS
                     IRSLLGYQYGSLHYKDKLDNASLTIIETHDFKDFNCLTWNDKSYLRQEVKMTH"
     gene            complement(1190..2092)
                     /locus_tag="VBD27_001481"
     CDS             complement(1190..2092)
                     /locus_tag="VBD27_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7025485.1"
                     /translation="MITSIVFDVDDTIYDQQAPYRIAVEKCFPDFDMSAINQAYIRFR
                     HYSDIGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEATGIYFQEIYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVKKLGLYDYVDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPSTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRSLKPGIKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKSNPVLEMGLNNGLMMAAERLLESNMS
                     VDKVVILLRLTAKQEKVLRMKYAR"
     gene            2267..3757
                     /gene="lysS"
                     /locus_tag="VBD27_001482"
     CDS             2267..3757
                     /gene="lysS"
                     /locus_tag="VBD27_001482"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO7025486.1"
                     /translation="MSNQHIEELNDQQIVRREKMAALTEQGIDPFGKRFERTATSGQL
                     NEKYADKSKEDLHDIEETATIAGRLMTKRGKGKVGFAHIQDREGQIQIYVRKDSVGEE
                     NYEIFKKADLGDFLGVEGQVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDIETI
                     YRKRHLDLISNRDSFDRFVTRSKIISEIRRFMDSNGFLEVETPVLHNEAGGASARPFI
                     THHNAQDIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEAYQ
                     AYADYQDIMDLTEGIIQHVTKTVKGDGPINYQGTEIKINEPFKRVHMVDAVKEITDID
                     FWKEMTLEEAQALAQEKNVPLEKHFTTVGHIINAFFEEFVEDTLIQPTFVFGHPVEVS
                     PLAKKNDTDPRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQASAKELGDDEATG
                     VDYDYVEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3832..4302)
                     /locus_tag="VBD27_001483"
     CDS             complement(3832..4302)
                     /locus_tag="VBD27_001483"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEO7025487.1"
                     /translation="MTIIEGQLVANEMKIGIVVSRFNELITSKLLSGAVDGLLRHGVS
                     EEDIDIVWVPGAFEIPYMARKMALYKDYDAIICLGVVIKGSTDHYDYVCNEVTKGIGH
                     LNSQSDIPHIFGVLTTDNIEQAIERAGTKAGNKGYDCALSAIEMVNLDKKLRER"
     gene            complement(4317..5510)
                     /locus_tag="VBD27_001484"
     CDS             complement(4317..5510)
                     /locus_tag="VBD27_001484"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEO7025488.1"
                     /translation="MAFSPIKKLLQDIKSGEMVVLMDDENRENEGDLICAAEMVTKES
                     INFMAKFGKGLICLPLSNYYAEKLELAQMASHNTDNHETAFTISIDHLSTSTGISAED
                     RALTAKMVANDSSKAKDFRRPGHLFPLLAKEGGVLARNGHTEATVDLCRLAGLKECGL
                     CCEIMAEDGSMMRKDELLAFAQKHDLAIATIKQLQDYRRQEEGGVVREIEIQLPTQFG
                     HFKAYGYSEVVANKEHVALVKGDISSGEDVLCRLHSECLTGDVFHSLRCDCGEQLANA
                     LQQIEAEGRGVLLYMRQEGRGIGLINKLKAYHLQEEGLDTLEANLALGFEGDERDYGV
                     SAQLLKDLGINSINLLTNNPDKIQQLEAEGICVKNRVPLQVAATAYDLNYLKTKKEKM
                     GHLLD"
     gene            complement(5528..6178)
                     /locus_tag="VBD27_001485"
     CDS             complement(5528..6178)
                     /locus_tag="VBD27_001485"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEO7025489.1"
                     /translation="MFTGIIEEMGQVSRIRNGIKSQQLSIDVPKLVPLLRKGDSVAVN
                     GVCLTVLDKSEKAFIADVMPESMMRTSLAALRLHSKVNLELALRSDSRLGGHFVLGHV
                     DGVGKIEKIQKDDIAVRFSIDAPPSIMSYIIEKGSVALDGISLTVVSFTEHSFEVSVV
                     PHTMAQTNLSLKKVGDLLNIEVDVLGKYAEKFLAPTNRTNHTSSVMDWSFLSENGY"
     gene            complement(6159..7268)
                     /gene="ribD"
                     /locus_tag="VBD27_001486"
     CDS             complement(6159..7268)
                     /gene="ribD"
                     /locus_tag="VBD27_001486"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEO7025490.1"
                     /translation="MMKKEDYMALALKEAEKGMGFVAPNPLVGAVIVKDDRIISKGYH
                     KRFGDLHAERQAIKNADEDISGSTLYVTLEPCCHVGKQPPCTEALIKSGIKKVVVGSL
                     DPNPLVSGKGIALLRKEGLNVEVGILREECDALNERFIFHMTYKQPFVYLKYAMTLDG
                     KIATKTGDSKWISNEHSRQSVQKLRQKCSAIMVGINTVLADNPRLTCRIPKGEALVRI
                     VCDSQLKIPLDSYLVKSAKTIPTWIATCSDNLAQQQTLKEMGCRLIKVPRKDGKLDLK
                     VLMTILGQEGIDSLLIEGGSSLHFSALEAGIVNRLIVFIAPKIIGGLKAKTAISGEGL
                     DWLNQAFRVKDIELSRMDSDVVIEGKVEHYVYRNY"
     regulatory      complement(7462..7576)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         2492 a         1591 c         1197 g         2479 t
ORIGIN      
        1 attagttaag ggtcattatg agtaaaaaga ataaaatcaa aaaaacttta gttgatcaaa
       61 ttttagataa agcaaaaatc gaacatgata gtctacaatt ggatgcttta cagggagatt
      121 tacccaacgg tattcaaaaa caagatatct ttaagacatt agcgctaatc ggtgataaaa
      181 ctggtcccat aataggtatc ttacctctca ccgagcattt atccgaaaaa aagttagcta
      241 aaatttcagg aaacaaaaag gtacaaatga tacctcaaaa agatctccag aaaataacag
      301 gttatattca cggagcaaat aatccaattg gaattcgcca aaaacataat taccctattt
      361 ttatcgatac tatcgcttta gaaaaacaag aactcattgt ctctgcagga gaaattggac
      421 gttctattcg tattaatagt gaggttcttg cagatttcgt aaatgctaaa tttgccgata
      481 taaaggaata ataattagga ttggagaaaa atatgaaatt ttatcttgtt agacacggaa
      541 aaacacaatg gaatttagaa gggcgtttcc aaggtgctaa cggagactca cctctccttg
      601 aagaagctat tgaagaatta gaagaacttg gacaatacct atcatctatt cattttgatg
      661 ctgtttattc tagcgactta ggtcgtgcta gagatactgt taatatcctc aatgatgcta
      721 attcatgccc aaaagaaatc cactatactt ctcagcttcg tgaatgggca ttgggaactt
      781 tagagggttg taaaattgca actatgcaag ctatctatcc tcgtcaaatg accgcatttt
      841 atcaaaatcc cttacaattt aaacatgaca tgttcggtgc agaatctctt tatcagacaa
      901 cgcatcgtgt agagagtttc ttacgttctt tagcttcaaa aaattacgac aaggtcctta
      961 ttgtaggaca tggagcaaat ctaactgcgt ctattcgttc cttactgggt tatcaatatg
     1021 gtagtttaca ctacaaagat aaacttgata atgccagcct cacaatcata gaaactcatg
     1081 attttaaaga cttcaactgt ctgacttgga acgataaatc ttatttaagg caagaggtca
     1141 aaatgacaca ctaaaataat ccactagatt catctagtgg attatttaat tatctagcat
     1201 acttcatgcg taatactttt tcctgttttg cagttaggcg cagtaaaata acaactttgt
     1261 caacgctcat attactctca agcagacgct ctgctgccat cattaaacca ttattaagtc
     1321 ccatttcaag gactggatta cttttatcgt tgatatcaaa aataaattta ttatcaggta
     1381 agtcaaacaa cactttaaca gcaccgaata attgctcaaa gttatcaata gcaacatcat
     1441 aaactggttt aattcccggt tttaaagaac gtcctctatg gttgaaccac atagaatgcc
     1501 aaccaccatt aaatgcaccc ataatatcat tatcatatga atcacccaca taaagtgtag
     1561 ttgaagggtt catatcaaat tgctctgctg ctaaattaaa aatctccttt tcaggctttt
     1621 gaaaaccagt tgcttgacta actataacac gttttggatc aacatagtca taaagtccca
     1681 attttttaac ttttttcaat tgatgttccg tagggccatt tgtaataatt cccattggta
     1741 cattttttga tttcaaaaag tcaagtgtca tacgcatctc atcaagcatt gtaatatttt
     1801 caagttcatg ttcgtatatt tcttgaaaat aaataccagt agcttcatca atttcacgat
     1861 aaccaaactc taaaagcgtt tctttgcatc gccagaaacg aaaatattct gttgtccatt
     1921 caccagccat tactcgtgga aagccaatat ctgaataatg acgaaagcga atataagctt
     1981 ggttaatagc gctcatatca aaatcaggaa agcatttctc cacagcaata cggtaaggtg
     2041 cttgttgatc gtaaattgta tcatcaacat caaaaacaat tgaagtaatc atgattatcc
     2101 ttttctttct gaattttcaa ttgttaatta tacctttttc tacccttatt ttcaaatggt
     2161 gaaaagtcta ttaaaaatga gaataacacc ataaactcat aaaataaatt atgaattatc
     2221 agtgtttttg tggtacaata ggatgaatac taacttggag gaaattatgt ctaaccaaca
     2281 tattgaagaa ttaaatgacc aacagattgt tcgtcgtgaa aaaatggctg ctttgactga
     2341 acaaggcatt gatccttttg gtaaacgatt tgaacgtaca gcaacatctg gtcaattaaa
     2401 tgaaaaatac gctgacaaat caaaagaaga cttgcacgac attgaagaaa ctgctacaat
     2461 cgctggtcgt ttaatgacta agcgcggtaa ggggaaagtt ggctttgcgc atatccaaga
     2521 tcgcgaaggt caaattcaaa tttatgtccg taaggattct gttggcgaag aaaactacga
     2581 aatctttaaa aaagctgatt taggtgactt ccttggtgtt gaagggcaag taatgcgtac
     2641 agatatggga gaactttcta tcaaggctac tcatattact cacctttcaa aagcactccg
     2701 tccacttcct gaaaaattcc acggcctaac tgatattgaa acaatctatc gtaaacgtca
     2761 tcttgattta atctcaaatc gtgacagctt tgaccgtttt gtaacacgct caaaaatcat
     2821 ttcagaaatt cgtcgcttta tggatagcaa tggtttctta gaggttgaaa cacctgttct
     2881 tcataatgaa gctggtggtg cttctgctcg cccatttatc acacatcaca atgcacaaga
     2941 cattgacatg gttcttcgta tcgcaactga attgcatcta aaacgtctta ttgtaggtgg
     3001 tatggagcgt gtttatgaaa tcggacgtat tttccgtaat gaagggatgg atgcaaccca
     3061 caatccagag ttcacatcta ttgaagccta ccaagcctat gctgactacc aggatattat
     3121 ggacttgaca gaaggtatta tccagcatgt tacaaaaact gtaaaaggtg atggaccaat
     3181 caattatcaa ggcacagaaa ttaaaattaa tgaaccattc aaacgtgttc atatggttga
     3241 tgcggttaaa gaaatcactg atattgactt ctggaaagaa atgactcttg aagaagcaca
     3301 agctttggca caagaaaaaa atgttcctct tgaaaaacat ttcacaacag ttggtcacat
     3361 cattaacgcc ttcttcgaag aattcgttga agacacttta attcaaccaa cttttgtctt
     3421 tggtcatcca gtcgaagtgt ctccacttgc taagaaaaat gatacagacc ctcgttttac
     3481 agaccgtttc gaactcttta tcatgactaa agaatatgct aatgctttta cagaattaaa
     3541 tgacccaatt gatcagctat ctcgctttga agcacaagca tctgctaaag aacttggcga
     3601 tgatgaagca actggagttg attacgatta tgttgaagca cttgaatatg gtatgccacc
     3661 tactggtgga ctaggtattg gtatcgaccg tctctgtatg ctgttaactg acacaacaac
     3721 tatccgagac gtcctacttt tccctacaat gaaataagaa tatattaaaa cccaccctta
     3781 tttaaggtgg gttttaatat tacctctact gaactttgtt aggtttctga tttatcgctc
     3841 acgcaatttt ttatcaaggt ttaccatttc aatagccgaa agagcacaat cataaccttt
     3901 atttcctgct tttgttcctg ccctctcaat agcttgttcg atattatccg ttgttagaac
     3961 accaaagata tgagggatat ctgactggct atttaaatga ccaatacctt ttgtcacctc
     4021 attacaaaca taatcataat gatcagtgct gcctttaatg actaccccaa gacagataat
     4081 agcatcataa tctttatata atgccatttt tctagccata taggggattt caaaagcacc
     4141 gggtacccat acaatatcta tatcttcttc tgaaacacca tgtctcaaca gtccatctac
     4201 tgctcctgat aataatttag atgtaatcaa ttcattgaaa cgtgagacta cgattcctat
     4261 tttcatttcg tttgccacta attgtccttc aataattgtc attttttctt cctcctttaa
     4321 tctaaaagat gtcccatttt ttctttttta gtttttaaat aattaaggtc ataagctgtt
     4381 gctgccacct gtaatggcac tctgtttttg acgcaaattc cttctgcttc aagttgttgg
     4441 attttatctg gattattggt tagtaagttt atagaattta tacctaaatc ttttaaaagt
     4501 tgagcactaa ctccatagtc tctctcatct ccttcaaaac ctaaagccaa attagcttct
     4561 aaagtatcta agccttcttc ttgcaaatga taagctttta acttattaat caaaccaatt
     4621 cctcgacctt cctgtctcat ataaagaagg acgccacgac cttcagcctc tatttgttgg
     4681 agtgcattag ccagttgttc cccacaatca catctcaagg aatgaaagac atcacctgtc
     4741 aaacattctg agtgtaaacg acataaaaca tcttcaccac tactaatatc ccctttaaca
     4801 agggcaacat gttccttatt tgccacaact tcactatatc cataagcctt aaaatggccg
     4861 aattgcgttg gtagctgaat ctcaatttct ctaaccaccc ctccctcttc ttgacgtcta
     4921 tagtcttgca attgttttat ggtagcgata gctaaatcgt gcttttgagc aaatgctaat
     4981 aactcatcct ttctcatcat actaccatct tcagccatta tttcacaaca aagtccacat
     5041 tcctttaatc cagctaaacg acatagatca acggttgctt cagtatgtcc atttctagct
     5101 aaaacacctc cttccttagc taacaaggga aataagtgcc caggacgtct aaagtcttta
     5161 gctttgctac tgtcgttagc aaccatctta gctgtcaaag ctctatcttc cgcagaaatc
     5221 ccagtactag ttgatagatg atcaatggat atggtaaaag ctgtttcatg attatcagta
     5281 ttatggctag ccatttgagc taattctaac ttttcagcat agtaattaga cagaggtaag
     5341 caaattaatc ctttcccaaa tttagccata aaattaatcg actcttttgt caccatttca
     5401 gccgcacaaa taaggtcccc ttcattctct ctattttcgt catccatcaa aactaccatt
     5461 tcccctgatt tgatgtcttg taaaagtttt tttattggac taaaggccat tcgctttctc
     5521 ctctctctta gtagccattt tctgataaaa aagaccaatc cattactgat gaagtgtgat
     5581 tagttctatt agtcggtgct aaaaatttct cagcatattt gcctaaaaca tccacttcga
     5641 tatttaataa atccccaacc ttttttaagg ataaattagt ttgagccatc gtgtggggaa
     5701 cgaccgaaac ttcaaaagaa tgctctgtaa atgataccac cgttaggcta atcccatcta
     5761 gagccaccga acctttctct ataatataag acataatact gggcggagca tcaattgaaa
     5821 atctgacagc aatatcatcc ttttggattt tttctatttt tccaacccca tccacatgtc
     5881 caagaacaaa atgcccccct aaccttgagt cacttctgag tgctaattct aagttaactt
     5941 tagaatgaag tcgtaaagca gctaagcttg ttcgcatcat actttctggc atgacgtctg
     6001 ctataaaagc tttttcactt ttgtcaagga ctgtcaaaca aacaccatta acagctacgc
     6061 tatccccttt tcttaataaa gggactagtt tgggaacatc aatacttaat tgttgggatt
     6121 ttatgccatt ccgaatacga ctgacttgac ccatttcctc aataattcct gtaaacataa
     6181 tgttctacct ttccttcaat aacaacgtca gaatccatac gactaagttc aatatctttt
     6241 actctgaaag cttggttaag ccaatccaag ccttctccag aaatagctgt ttttgctttc
     6301 agacctccta ttattttggg tgcaataaaa acaattagtc tgttaacaat accagcttct
     6361 aaagctgaaa aatgtaaact acttcctcct tcaataagaa ggctatcaat cccttcttgt
     6421 cctaaaatag tcattaatac tttcaaatcc agctttccat cttttcttgg gaccttaatc
     6481 aaacggcaac ccatttcttt taatgtttgt tgctgagcaa gattatctga acatgttgca
     6541 atccatgttg gtattgtttt agcgctctta actaggtaag agtctaaagg tatcttcaac
     6601 tgactatcac atacgatcct cacaagcgct tcacctttag gaatacggca agttaaacgt
     6661 ggattatcag ctaatacagt attgataccg accataatag cactacattt ttgcctcaat
     6721 ttctgaacag attgtcttga gtgctcattg gatatccact tagaatcgcc cgtctttgta
     6781 gctatcttcc catctaaagt catagcatat ttcaaataaa caaaaggctg cttataggtc
     6841 atgtgaaaaa taaatctttc attcagagca tcgcattctt ctcttaggat accaacttca
     6901 acatttaaac cttcttttct taacaaggca atcccttttc ctgaaactaa aggatttgga
     6961 tctaaacttc caactactac ttttttgata ccagatttaa taagagcttc agtacaaggt
     7021 ggttgcttac ccacatgaca gcatggttct aaagtcacgt acagagtact tccagagata
     7081 tcctcatcag catttttaat agcctgtctt tccgcatgta aatctccaaa tcgtttatga
     7141 taacctttgg aaattatacg atcatcttta acaataacag cacctactaa tgggttggga
     7201 gcaacgaacc ccataccttt ctcagcctct ttcaaggcta atgccatgta gtcttctttt
     7261 ttcatcattt cctttttcct tctaaaataa aaaaccttag cttctaaaat agaaaactaa
     7321 ggggagggat gagaagtcgt tgattatact aattagcatt atattttagg tagcacaaaa
     7381 aaactaaaga ctccctatag aaaactatag ggagtctctc attaatccag cacaaataaa
     7441 ctgcaagtag cagacaatca ctgaattctt ctctcatcca gactttactg tcggtactgg
     7501 aatcacacca gttcatgctc gaaagctcgc ggactatacc gccggttagg attttcacct
     7561 gaccccgaag aaattctatt tgacagttat tttactaaac tcatttttat ttgtcaataa
     7621 aaataatgtg ttttttatta taaaataata attaagtagc tcatctattc ttatctttaa
     7681 aagacagtat atctatacca taaagcaatc tttcgaacta atcaaacaag aatcgatagt
     7741 attattggat tatatttct
//
LOCUS       DATRQA010000094         7747 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.094, whole genome shotgun sequence.
ACCESSION   DATRQA010000094 DATRQA010000000
VERSION     DATRQA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.094"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            29..2266
                     /gene="metE"
                     /locus_tag="VBD27_001487"
     CDS             29..2266
                     /gene="metE"
                     /locus_tag="VBD27_001487"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEO7025491.1"
                     /translation="MVKVSNLGYPRLGEQREWKQAIEAFWAGNLEQKDLEKQLKQLRI
                     NHLKKQKEAGIDLIPVGDFSCYDHVLDLSFQFNVIPKRFDEYERNLDLYFAIARGDKD
                     NVASSMKKWFNTNYHYIVPEWEVETKPHLQNNYLLDLYLEAREVVGDKAKPVITGPIT
                     YVSLSSGIVDFEATVQRLLPLYKQVFQDLIDAGATYIQIDEPIFVTDEGELLVDIAKS
                     VYDFFAREVPQAHFIFQTYFESAVCLDKLSKLPVTGFGLDFIHGRAENVAAVKQGLFR
                     EKELFAGIVNGRNIWAVNLEETLALLEEIGPFVKRLTLQPSSSLLHVPVTTKYETHLD
                     PVLKNGLSFADEKLKELELLASAFDGNKTKGYHEALSRFSALQAADFRHVALESLAEV
                     RLERSPYKLRQALQAEKLQLPILPTTTIGSFPQSPEIRKKRLAWKRENLSDSDYKDFI
                     KTEIRRWIAIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKLGWVQSYGSRAVKP
                     PIIYGDIKHIQPLSLEETVYAQSLTKKPVKGMLTGPITITNWSFERDDISRSDLFNQI
                     ALAIKDEIQLLEQSGIAIIQVDEAALREGLPLRQQKQQAYLDDAVAAFKIATSSVKDE
                     TQIHTHMCYSKFDEIIDSIRSLDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDI
                     HSPRIPTKEEIIVNIQRSLKCLSKEQFWVNPDCGLKTRREAETIAALEVLVSATKEVR
                     QQLDN"
     gene            2311..4155
                     /locus_tag="VBD27_001488"
     CDS             2311..4155
                     /locus_tag="VBD27_001488"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEO7025492.1"
                     /translation="MSKFLEKLKTDILVADGAMGTLLYTYGLDTCHESYNVTHPEKVL
                     AIHQAYIEAGADVIQTNTYGAQRHRLKNYGLEDQVVSINQAAVNIAHQATLGKETFIL
                     GTVGGFRSQRQCDLTLDNIVEETLEQVEALLVTGQLDGLLFETYYDIEEITTVLKIVR
                     EMTDLPIITNISLHEAGVTSNGKPIVEALSQLVMLGADVIGLNCHLGPYHMIQSLKQV
                     PLFAQSYLSVYPNAGQLSLDGENSQYQFSQNSEYFGKSAELLVAEGVRLIGGCCGTTP
                     DHIRAVKRSIRGLKPIERKVVTPIIPVKDFVRRIRRTDTLVDKVKKEVTIIAELDPPK
                     HLDIVQFQKAIRAIDQKGIAAITLADNSLSNTRICNLSIASLLKDEISTPFLLHIACR
                     DHNLIGLQSRLLGMELLGFNHILAITGDPTKLGDFPGATSVYDVTSFKLLSLIKQLNQ
                     GLSYSGASLRRPTDFTVAAAFNPNVKNLTRTVKLIEKKVASGADYFMTQPIFDHSVLR
                     ELADLTKTVEQPFFIGIMPITSYNNAVFLHNEVPGIKLSESFLSALEKVKDDKEACLT
                     LALNESKSLIDEALNHFNGIYLITPFLRYDLTLELIDYIQKKQVRKSS"
     gene            4502..5209
                     /locus_tag="VBD27_001489"
     CDS             4502..5209
                     /locus_tag="VBD27_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEO7025493.1"
                     /translation="MVCVDFIDQKVYSEAEGLFVSDLDLGIKGLIRARYPFALDSDFI
                     SYGNLSHYCMNYLDEIVQRANQKNEKIKYNVTTLMKEEEKPFNVEERLNKQATIGQRI
                     ADDVARFGGSWTFIIVFVSIMAIWMLVNIMKPFGIQFDPYPFILLNLALSTIAAIQAP
                     LIMMSQNRAADYDRLQARNDFNVNKTSELEIRLLHEKIDHMVQQDQFELLEIQKLQTE
                     MLVSLGNQLAQLKQLQK"
     gene            complement(5374..6459)
                     /locus_tag="VBD27_001490"
     CDS             complement(5374..6459)
                     /locus_tag="VBD27_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO7025494.1"
                     /translation="MTTNVMANYSYGEDCFKEIPSVLATYRIQSIAFVGGKRALDSSE
                     AEVRSILEEANIKVTGSFVYGTDSTQSNIDKLVANPQVQAADAILGFGGGKALDTAKM
                     VAKELGKNSFTIPTICSNCSAGTAIAVVYNDDHSFLRYGYPESPLHIFINTRIIAQAP
                     SKYFWAGIGDGISKAPEVERATLEAKTNKLPHTAVLGQAVALSSKEAFYQFGEQGLKD
                     VEANLASRAVEEIALDILISTGYASNLVNQPDFYYNSCHAHAFYYGTTAIQRQGEFLH
                     GVVVAFGVLVLHAYFNELEELEKVARFNKSLGLPTTLADVSLSEKDIPKIVEIAMTTN
                     EYKNTPFDPKMFAQAILAADAFGQTLA"
     gene            complement(6660..7199)
                     /locus_tag="VBD27_001491"
     CDS             complement(6660..7199)
                     /locus_tag="VBD27_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEO7025495.1"
                     /translation="MKIAIIGYSGSGKSTLARKLGNYYNCNVLHLDSIHFAPNWEERK
                     YDDMIDDVSSMLEKRTWIIEGNYKKLLYQERLADADEIIFFDFNRFNCLWRAFKRYCK
                     FRGKTRPDMANGCPEKLDFEFISWILKDGRSDKQKSNYKQVVEDYPQKIKVLKHQRDL
                     DQYLKELPVASNTDPLKSL"
BASE COUNT         2487 a         1251 c         1483 g         2526 t
ORIGIN      
        1 tattagctat aacttgatgg aggaaattat ggtaaaagtt tcaaatttag ggtatccacg
       61 tcttggtgaa cagcgcgaat ggaagcaagc gatcgaagct ttctgggcag ggaatcttga
      121 acaaaaagat ttagaaaaac aactaaaaca attacgtatc aatcatttaa agaaacaaaa
      181 agaggcaggt attgacctta ttccagtggg ggatttttct tgttatgatc atgttttgga
      241 tttgtcattt caattcaatg taatcccaaa gcgtttcgat gagtatgaga ggaatttaga
      301 cctttatttt gctattgcaa gaggtgacaa agataatgtc gcatcatcta tgaaaaagtg
      361 gtttaatacc aactaccact acatagtccc agaatgggag gttgagacta aacctcactt
      421 gcagaataat tacttacttg atctttatct agaagctagg gaagtagttg gtgataaagc
      481 aaagccggtt atcactggtc caataaccta tgtttcctta tcatcaggaa ttgtcgactt
      541 tgaagcgact gttcagcggt tattaccact ttataagcag gtctttcaag atctgataga
      601 tgcaggcgcc acctatattc agattgatga gccgatattt gtaactgatg aaggtgaact
      661 tttagtagat atagctaagt ctgtctatga tttttttgca agagaagtac cacaagccca
      721 cttcatcttt caaacctact ttgaatcagc agtctgttta gataaactct ctaagctgcc
      781 agtaacggga tttggccttg attttataca tggtagggcg gaaaatgtag ctgctgttaa
      841 gcaaggtcta ttccgcgaaa aagaattatt tgcaggaatt gttaatggtc gaaatatctg
      901 ggcagtaaat ttagaagaaa cgttggcttt attggaagag ataggtccct ttgttaaacg
      961 tttgaccctt caaccttctt caagtctttt acatgtaccg gtgacgacta aatacgaaac
     1021 acatttagac cctgtgttaa agaatggctt atcatttgct gatgaaaaac taaaggaatt
     1081 agaactatta gcgagtgctt ttgatggtaa taaaacaaag ggatatcacg aagctttatc
     1141 tcgtttttca gctcttcaag ctgctgattt tcgtcatgta gcgttggaat cattagcaga
     1201 agtaaggctt gaacgaagtc cgtataaatt acgccaagct ttgcaagctg aaaaattaca
     1261 gttaccgatt ttaccaacaa caactattgg gtcctttcct caatcacctg aaattaggaa
     1321 gaaacgcctt gcttggaaaa gagaaaatct atctgactca gattataaag atttcataaa
     1381 aactgaaatt agaagatgga ttgctattca agaagatctt gatcttgatg tgttagtaca
     1441 tggcgaattt gagcgcgtgg atatggttga attttttggt caaaagttag ctggttttac
     1501 gacaaccaaa ttaggctggg tacagtctta tggttcaagg gcggtcaaac cacctatcat
     1561 ttatggtgat atcaaacata ttcaaccctt aagccttgaa gaaacggttt atgcccaaag
     1621 cttgactaag aaacctgtta aaggcatgtt gacaggtcct attactataa cgaactggtc
     1681 atttgagcga gatgatatca gccgatctga tctttttaat caaattgctt tggctataaa
     1741 agatgagatt caacttttgg aacaatcagg tattgctatt atacaagtgg atgaagcagc
     1801 ccttcgagaa ggcttaccct tacgccagca aaagcaacag gcttacttag atgatgctgt
     1861 tgcggccttt aaaattgcaa cttcatctgt gaaagatgag acacaaattc atacacatat
     1921 gtgttattca aaatttgacg aaattattga ttctatccgt tcactagatg cagatgttat
     1981 ttctattgaa acgagtagaa gtcatgggga catcattgaa agttttgaaa cagcagttta
     2041 tcctctagga attggtctgg gtgtttatga tattcattct cctcgcatac ctactaagga
     2101 agaaattatt gtcaatattc aacgatcact aaaatgtcta tcaaaagagc aattttgggt
     2161 aaaccctgac tgtggcttaa aaacacgtcg tgaagcagaa acaattgctg ccttggaggt
     2221 tcttgtttca gctaccaaag aggttcgtca gcaattagat aattaatagg ttaatcagta
     2281 aaggattgcc atgagggagg agtaaagaag atgtccaagt ttttagagaa attaaaaaca
     2341 gatattctag tagctgacgg tgcaatggga actctccttt atacttatgg gctagatact
     2401 tgtcatgaat cctacaatgt aacacatcca gaaaaggttc tagccattca tcaagcttat
     2461 attgaagcgg gtgcagatgt tattcagacg aatacctatg gggctcaacg tcatcgatta
     2521 aagaactacg gtttggaaga tcaggttgtc tctatcaatc aagcagcagt taatatagct
     2581 catcaagcga ctttgggtaa agaaacattt attttaggta ctgttggagg ttttcgttct
     2641 caaagacaat gcgatttaac attggataat attgtagaag aaactttgga acaagttgag
     2701 gctttattgg tgacaggaca gctagacgga ttattgtttg aaacttatta tgatattgag
     2761 gagataacga cagttttaaa aattgtgagg gagatgactg atctaccaat tattactaat
     2821 atttctcttc atgaagcagg ggtaacatca aatggaaagc ctattgtaga ggcgctcagc
     2881 caattagtta tgctaggtgc agatgtgatt ggcttaaatt gtcaccttgg accctatcat
     2941 atgatccaat cattgaagca agttccttta tttgcccagt cttatttgtc agtttatcct
     3001 aatgcaggtc agttatcttt agatggtgag aatagtcaat accagtttag tcagaattca
     3061 gagtattttg gaaaatctgc agaacttctc gtagcagaag gagttaggct tattggtggt
     3121 tgttgtggga caactccaga tcatattaga gctgtgaaac gatctattag aggattgaaa
     3181 cctattgaac gaaaggttgt tactcctata atacctgtaa aagattttgt taggcgtatc
     3241 agacgaacag atactctagt ggataaggta aaaaaagaag taacaattat cgcagaatta
     3301 gatcctccaa aacatttaga tatcgttcag tttcaaaagg ctatacgagc gattgaccaa
     3361 aaaggtattg cagcaattac tttagcagat aattcactat ctaatacacg tatttgtaat
     3421 ttaagtatag ctagcttgct taaagacgaa attagtacac cctttttact acatatagct
     3481 tgtcgtgacc ataatttaat tggtttacag tctagactgt tgggcatgga attattaggt
     3541 tttaaccata ttttagccat aacaggagat ccgactaaac ttggtgattt cccaggagcg
     3601 actagtgttt atgatgtgac ttcctttaaa ctcctttctc tgattaaaca acttaatcaa
     3661 ggtttatctt atagtggtgc tagcttgaga cgtccaacag attttacagt tgctgctgcc
     3721 tttaatccta atgtgaaaaa ccttactcga accgtaaaat tgattgagaa aaaagtagct
     3781 tctggtgctg attattttat gacacaacca atatttgatc actctgtgtt aagggaatta
     3841 gctgatctta ctaaaacagt tgaacaaccc ttttttatcg gaattatgcc aataacaagt
     3901 tataataacg ctgtttttct acacaatgaa gttcctggta ttaaactttc tgagtcattt
     3961 ttatctgctc ttgaaaaggt aaaggacgac aaagaagctt gtttaacttt ggccttaaac
     4021 gagagtaagt ccttgattga tgaagccttg aaccatttta atggtatcta tttgattact
     4081 ccctttttac gctatgatct gactcttgag ttaattgact atatacaaaa aaagcaagta
     4141 agaaagtcct cttaactttc ttacttttta gttggtcgtt tattattata ataaaagtta
     4201 cgtaccaaaa cagaggttaa actagatttt taagccactt tctatactaa gataaattag
     4261 ttaatttaag ttttctttaa gtttatagac ttagaataaa gcgttatttt gatgggtagc
     4321 ataaaatcaa ggcataatat agatgtcagc gattttttta gactaacgaa ataacgatgt
     4381 aaagcttaat aggggtgtat cgaatactca cttatatagt accaaaagcc tatagcctgt
     4441 tggctagctt gaccgtattc aaatgttgaa taaatcttgg ttgagaaagg gaggtataaa
     4501 aatggtatgt gtcgatttta ttgatcaaaa agtttatagt gaagcagaag gtctatttgt
     4561 ttccgattta gacttaggca ttaaaggttt aataagagca cgttatcctt tcgctctgga
     4621 tagtgacttt atttcgtatg ggaatttaag tcattattgt atgaattatc ttgatgaaat
     4681 tgttcagcga gcgaatcaga aaaatgagaa gataaaatat aatgtgacta ccttgatgaa
     4741 agaagaagag aagcccttca acgttgaaga gcgtttgaac aagcaagcaa ctatcggtca
     4801 acgaattgct gatgatgttg cacgttttgg cggatcttgg acttttatta tcgtttttgt
     4861 atccattatg gctatttgga tgttggttaa tatcatgaag ccttttggta ttcaatttga
     4921 tccttaccca tttatcctgt taaacttagc tttatcaacg attgctgcga tccaagcacc
     4981 acttattatg atgagtcaaa atcgtgctgc tgattatgat cgtttgcaag ctcgaaatga
     5041 ctttaatgtc aataaaacct ctgagttaga aattagatta ttgcacgaaa aaattgacca
     5101 tatggtacaa caagatcagt ttgaattgtt agagattcaa aagttacaga cagaaatgct
     5161 agtatcattg ggaaatcagt tagcacaatt gaaacaacta caaaaataat cttttagcag
     5221 ttatttaaaa atactgatac aatactaaga atttaaatta atactgcaac ctcctataat
     5281 ggagagcagt atttttactt tattaataac aaaaacccct tagatgatgt gtctaaggga
     5341 tttttgtatc ggaataatcg agttggtgaa ttattaggct agtgtctgtc cgaaagcgtc
     5401 tgctgctaaa atagcttgag caaacatttt gggatcgaaa ggggtatttt tgtattcatt
     5461 agtagtcatt gcaatttcaa caatttttgg aatatctttt tctgataaag agacgtcagc
     5521 tagagttgtt ggcaaaccta gtgacttatt aaaacgagct actttttcaa gttcttctag
     5581 ttcgttgaaa taagcatgta aaactaacac accaaacgca actacaacgc catgtaagaa
     5641 ttcaccttgt ctttgaatag cggttgtacc atagtaaaat gcatgcgcgt gacaagaatt
     5701 gtagtagaaa tcaggttgat taactaaatt agaagcatag ccagttgaga ttaagatatc
     5761 aagcgcaatt tcttcaactg cacgcgaagc taaattagct tcaacgtctt ttagaccttg
     5821 ttcaccaaat tgataaaaag cttcctttga agacagagcg actgcttgtc ctaacactgc
     5881 agtatgtggt agtttattgg tcttagcctc taaggtagca cgttctactt caggggcttt
     5941 tgaaataccg tccccaatac cagcccaaaa atatttgctt ggtgcctgtg caatgatccg
     6001 tgtattgata aaaatatgaa gtggagactc gggatagccg tatcttaaga aagaatggtc
     6061 atcattatat acaactgcaa tggcagtacc tgcggagcaa ttagaacaaa tagttgggat
     6121 agtaaagcta tttttaccta actcttttgc taccatttta gctgtgtcta aagcttttcc
     6181 accgccaaaa cctaagatag catcagcagc ttgaacttgt gggttggcta ctaacttgtc
     6241 aatgttagat tgtgttgaat ccgttccata gacaaagctt cctgtgactt taatatttgc
     6301 ttcctctaaa atagagcgaa cctcggcttc tgagctgtct agggcacgtt tgccacccac
     6361 gaaagcaatg ctttgaatac ggtaagttgc caaaacgcta ggaatttctt tgaagcaatc
     6421 ttctccgtag ctataatttg ccattacatt tgttgtcatt cagtgtcctc ctctattcca
     6481 aaggtcgatt agagacagct attttctaaa agtgaatagt tttttgtctc ttgaccaagt
     6541 gattatcata ttatagcaga atattcagaa aattggaaca tgaaaaataa tttaattttc
     6601 aagaataaga atagaaaagg taaagcgaga tatacgagta aatactgtca gtataataat
     6661 tataagctct tgagagggtc tgtattggat gcaacaggga gttcttttag gtattgatca
     6721 agatcgcgtt ggtgttttag cacctttatt ttttgagggt agtcctcaac aacttgttta
     6781 tagtttgatt tttgtttgtc agagcgaccg tctttaagaa tccaactgat aaattcaaaa
     6841 tctaattttt ccgggcatcc attggccata tcaggtctag tcttaccacg aaacttacag
     6901 taacgtttga atgctctcca caagcagttg aagcgattaa agtcaaaaaa aataatctca
     6961 tctgcatctg ctagacgttc ttgatatagc agttttttgt agttaccttc tataatccat
     7021 gttctttttt ctaacatgct gctgacatcg tcaatcatgt catcatattt tctctcttcc
     7081 caatttgggg cgaagtgaat actatcaagg tgtaatacat tgcaattata gtaatttccc
     7141 aattttctcg ctaaagtgga cttacctgac ccactatatc cgattattgc tattttcata
     7201 tactacctcc gttaaattgt ttacttgaag ttaatgctgt acttaggatt tacttatttt
     7261 ataagggtta ttcacttttt tagattaata tatttttgat ataagttatc catacacatg
     7321 gtaacataat taccagtaaa tgtaatctag atatcaatac tatggttgac gatattgtta
     7381 acttttgggt tcaaaaaatt ggggtaaaaa tcatcggagg gaactgccaa acaaactcac
     7441 agtttaaaat actaagtaat aaaaagtctt gaatttatta tgtttaaggc tttttaagcg
     7501 tctaaaatac ttagtctagt atgaatttct aaacagaaag taaaaataat gagttttagt
     7561 atggtttttg ggcataatgg aacgatttta tctaaaatag tacgtttctt attaaattta
     7621 tttttttaga aaaacattag gctaacctat ttgataaaaa tagtgataag tagaaatttt
     7681 ctaaaatatt tagatttata taaggtggta aattatcgtt atggtttttt acatgaaaat
     7741 ctaaatt
//
LOCUS       DATRQA010000095         7692 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.095, whole genome shotgun sequence.
ACCESSION   DATRQA010000095 DATRQA010000000
VERSION     DATRQA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7692
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.095"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            53..3472
                     /gene="srr2"
                     /locus_tag="VBD27_001492"
                     /pseudo
     CDS             53..3472
                     /gene="srr2"
                     /locus_tag="VBD27_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086874280.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin Srr2"
     gene            3582..4835
                     /gene="secY2"
                     /locus_tag="VBD27_001493"
     CDS             3582..4835
                     /gene="secY2"
                     /locus_tag="VBD27_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEO7025496.1"
                     /translation="MIGKLYYSYRKSTLLRSILWTILIVGVYMLGQRVLLSTVPLSHQ
                     EIKLAVDQHLLNNFSAVSGGSFNKLNVFTLGLSPWMSSMIIWRFVSLFSWAKNATKRK
                     AEVAQYTLMLTISVIQAYGVSGNQFIKSSLLGSYSDIVFKALTMVLLISGSFVLMWLA
                     NLNASKGLGGSIVIILVSMLQATTTGIITYFEQSHFSIMDLLGTIIAISIILSVLLLI
                     STVVYRAEYRIPIRRIDIVSKFAQDTYIPIRINPAGSYPFMYGMTLMMLPTFIIQALL
                     LAFPNERLLIQISSMIAINKPLGIGLFVILLFILSIGFAYFNYDPYNIAKNMRQRGEY
                     FQNVTPGAATQRFLQEKISIMAVVGAIFTVIMGGLPMLAVVGKTNQVSIAMIVINIYI
                     ISTFMLSIIEQVNTLKLWGSHRNII"
     gene            4845..6422
                     /gene="asp1"
                     /locus_tag="VBD27_001494"
     CDS             4845..6422
                     /gene="asp1"
                     /locus_tag="VBD27_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO7025497.1"
                     /translation="MFYFIPSWYNSDQKWHNEAPIWYRVLENMTFDDTVSQVRMFQNA
                     DEEVRLLLFSYNPQLRYMLHEESMLAVSYWSFFDDIQNIQQKEIKLINFKELNWPKGT
                     YFVYTPFLVLAKHGGEIIARIQFAENGNLLLIELLIDGVLDKIMIFDDRGFVSSIKYF
                     KQNRLIYQDYLNQNGVWQIREYASKFPRIVVNPMSDKVFNFTEYDDWDTLIRERILVL
                     KKLEVTPDDYIIVAVNDIHNNLVRKSFPDQKKIFSFFGERYSFDNRLDFNELLDEGEL
                     FIAESEKIERLLITSLQEKNYSSNYVENHISRIPPFDTRLRLGQSQNIEELIAYLLVD
                     DLSQTYLSEVLDQLLAKMEVNPSLELKIVSYKNDYDMTSLKEQVKDKILSSKNPDYFF
                     TSLSLEDENHFKEEQKTKIRGISFDIYTNENQIIDALDTARLVIDLRDDPDLYTQIAA
                     LSSGIPQINKVHTNYVEHQKNGWIISDSTELNNAIDYYFDGLMNWNKSLVYSVKKMGD
                     YTSGKILQRWRKLLEKE"
     gene            6424..>7692
                     /gene="asp2"
                     /locus_tag="VBD27_001495"
     CDS             6424..>7692
                     /gene="asp2"
                     /locus_tag="VBD27_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO7025498.1"
                     /translation="MEKLKILQIGEEPWDADLITPDNIEWLYCNGNAIQIFLEKLKSK
                     ELEKNNVEYIGKTSTNEVKLWFNAVILTSEVSELQLNLLSDCLEAHTLFYDEDFQLDF
                     CSENGIFKRKVLRPLPFNGSRKEKIIFLSRTLFSEQYGAKLKVSDIDLNPDFKGEIIY
                     EGNSSICFSGNFGEDFQPLFSFRYNLSSTDKAIEIWQEYQKLQGDNSIMIEVIQYQKG
                     SLDLILNTKYLSENDLADPYILEYDESVGFSSISIFARGDGVVRFGALHWRYSRKGLG
                     QFLIGGERYSDSKRQEFIYYFNPGDMKPPLNVYFSGFRGAEGFEGFYMMKRLGAPFLL
                     IGDPRIEGGSFYLGTSEYESELENVLQKYLDYLQFDHCQLILSGLSMGSFGALYYGST
                     FSPHAIVVGKPFTNLGDMVVNLKLRRPDDFE"
BASE COUNT         2561 a         1197 c         1420 g         2514 t
ORIGIN      
        1 tagaattaga aaagtttttt atatagcatt attttttaaa tatggaggaa aaatgttaaa
       61 aaagcagttt ggaaactttg gtgaaaaatc acgcaaagtt cgagttaaaa tgaggaaatc
      121 aggtaagcac tgagtcaaaa gtgttatgac acaaattggc tatgttatcc tttcgaggtt
      181 tagcggtaaa gagaaaagct ctaaggttca aactactagt gaagatttaa gcagaactaa
      241 aacgtctgct agcatcctaa cagcagtcgc agctcttgga gctgtagttg gagggacgac
      301 agacacaact tctgtctcag ctgaagaaac acctactgca acagaactta caggaaatga
      361 aaaaacatta gctactgctg aaactgtagt ggttgcgcca gaagtaaaaa ctgtaaactc
      421 agattcttca tcacattcaa caagtgaatc acaatcaatg tcaactagta ccttgcaatc
      481 taccagcgcc tcattatctg ccagtgaatc actaatggat tctacctctg cttctttatc
      541 tgagtctagc tcgttatcag aatatagttc tttatctcta tctagtagtg agagtgtctc
      601 agcatcagaa tcagttcaat catcagaagc ggcaacgacc gctagagttc aaccgagagc
      661 gatgagagtt gtatctagtg cttcagatat ggaaacttta ccagcagcat taatctctgg
      721 tgaaggagat gtaacaactg ttcaaggaca agatgtaaca gataagttac aaaatttaga
      781 tataaagctc tctggaggag tgcaagcaaa agcaggtgtt ataaatatgg ataagtcaga
      841 aagtatgcac atgtctttaa aattcactat tgattctgtg aatagaggtg atacttttga
      901 aattaagtta tcagataata tcgatacaaa tggagcttct aactattcta ttgtagaacc
      961 tataaaatca cctacgggtg aggtctatgc gactggaatt tatgattctc aaaaaaaatc
     1021 tatagtctat agttttacag attttgcggc ttcaaaaaat aatattaatg gaatattaga
     1081 tattccatta tggccagatg atacgacagt tcaaaacaca aaagaagatg ttcttttttc
     1141 ggtaaaaata aaggatcaag aggctacaat taaagaaaca gtgaaatatg atccaccggt
     1201 aagaattgat tttgcagggg gagtaagtgt agattctcga ataaccaaca ttgatgatgt
     1261 gggaaaaaag atgacttata taagtcaaat taatgtagat ggaaaatcac tctataatta
     1321 caacgggtta tatactagga tatataatta tagcaaagag agtacagcag atttaaagaa
     1381 ttcaacgata aaaatctata aaaccacctc ggataatatt gtagagagta tggtacagga
     1441 ttattcaagc atggaggatg taactagtaa gtttgcaaat agttacccag aaaaagggtg
     1501 gtatgatatt tattgggggc agtttattgc atcaaatgaa acgtatgtaa ttgttgtaga
     1561 aacaccattt actaatgcag taactttgaa tactacttta tcagattata atgagaacaa
     1621 tggtgtagaa cataatcata cttactcatc tgaatcggga tattcagatg taaatgctca
     1681 agaaagaaaa attttatccg aattagtaag tagctcagaa tcagtatcaa gttcagaatc
     1741 ggtatctaat tccgaatcta tttcaacttc tgaatcggta tctaactccg agtctatttc
     1801 aagttccgaa tcggtatcaa gctcagaatc tatttcaact tctgaatcgg tatcaacttc
     1861 agagtctatt tcaagctccg agtcagtatc aagttcagaa tcggtatcaa gctcagaatc
     1921 tatttcaagt tccgaatcgg tatctaactc cgagtctatt tcaagctccg aatcggtatc
     1981 taactccgaa tccatttcaa gttctgaatc ggtatcaagc tcagaatcta tttcgaattc
     2041 agagtctatt tcaagctccg agtcagtatc aacttcagag tctatttcaa gctccgaatc
     2101 ggtatctaac tccgaatcca tttcaagttc tgaatcggta tcaagctcag aatctatttc
     2161 gaattcagag tctatttcaa gctccgagtc agtatcaact tcagagtcta tttcaagttc
     2221 cgaatcggta tctaactccg aatccatttc aacttccgag tcagtatcta cctcggaatc
     2281 tatttcaagt tctgaatcgg tatcaagctc agaatctatt tcaagttccg aatcggtatc
     2341 aaactccgag tctatttcaa actccgagtc ggtatctagc tccgagtcag tatcaaattc
     2401 agagtctatt tcaagttccg aatcggtatc taactccgaa tccatttcaa cttccgagtc
     2461 agtatctacc tcggaatcta tttcaagttc tgaatcggta tcaaattcag agtctatttc
     2521 aagttccgaa tcggtatcta actccgagtc tatttcaagt tcagaatcgg tatcaaattc
     2581 agagtctatt tcaagttccg aatcggtatc aacttcagag tctatttcaa gttcagagtc
     2641 agtatcaagt tcagagtcag tatcaagctc ggaatctatt tcaacttcag aatcggtatc
     2701 taactccgag tctatttcaa gttccaaatc ggtatctaat tctgaatcta tttcaagttc
     2761 cgaatcggta tcaaattcag agtctatttc aagttccgaa tcggtatcaa actccgagtc
     2821 tatttcaagt tccgagtcag tatcaagctc agaatctatt tcaagttccg aatcggtatc
     2881 tagctccgag tcagtatcaa cttcagagtc tatttcaagt tccgaatcgg tatctaactc
     2941 cgagtctatt tcaagttccg aatcggtatc aacttcagag tctatttcaa gttcagaatc
     3001 ggtatcaaat tcagagtcta tttcaagttc cgagtcagta tcaagttcag agtcagtatc
     3061 aagttcagag tcagtatcaa gctcggaatc tatttcaact tcagaatcgg tatctaactc
     3121 cgagtctatt tcaagttcca aatcggtatc taattctgaa tctatttcaa gttcagaatc
     3181 ggtatcaaat tcagagtcta tttcaagttc agagtcagta tcaatgagta ctacagagtc
     3241 tctaagtgaa tcagaagtat caggggattc tgaaattagc tcaagtacag aatcatcaag
     3301 tcaatctgaa tcgatgaatc atactgaaat taaatccgat tccgaatctc aacacgaagt
     3361 taagcatcaa gtattaccag aaacaggtga taactcggct tcagcattag gtctgttagg
     3421 tgcaggattg ttgttaggag ctactaaatc tcgcaagaag aaaaaagatt aattttcaaa
     3481 gtgatattta gatgataaat cgaaagtact ttcgatttat cacttaagat agtaagtagt
     3541 tatactgtct taagtgataa attgatttga aaagggaaaa aatgattgga aaattatatt
     3601 atagctatag aaagtcaacg ctattaagaa gtattttatg gactatttta attgttggtg
     3661 tatatatgtt aggacaacgt gttttattat ccactgttcc tttatcacat caagagataa
     3721 aactagcagt agatcaacat ttactcaata acttttcagc agtaagtggt gggagtttta
     3781 ataaattaaa tgttttcaca ctggggttga gtccatggat gtcaagtatg attatttgga
     3841 gattcgtttc cttattttcg tgggcaaaaa atgcaacgaa gcgaaaagca gaagtagctc
     3901 aatatacttt aatgcttact atctcagtta tacaagcata tggtgtttca ggaaatcaat
     3961 ttataaaaag ctctttatta ggttcttata gtgatattgt ttttaaagca ttgacaatgg
     4021 tcttgttaat aagtggctca tttgtgttaa tgtggcttgc taatctcaat gcttctaaag
     4081 ggctaggagg aagtattgtt atcatattag tgagcatgct ccaagcaacg actactggaa
     4141 taataactta ttttgaacag tcacatttta gtattatgga tctgctggga actattatag
     4201 ctataagtat aatacttagt gttttattat taatctcaac ggtggtttat agggcagaat
     4261 atcgtatacc tattaggcgt attgatatcg ttagtaaatt tgcccaagat acctatattc
     4321 ctattcgcat aaatccagca gggtcctacc ctttcatgta tgggatgaca ttaatgatgc
     4381 ttccaacgtt tataattcaa gcattgttac ttgcttttcc aaacgagcga ttattgattc
     4441 agatctcgtc aatgattgca attaataagc ctttaggtat tggacttttt gttatattat
     4501 tatttatttt gagcattgga tttgcatatt ttaattacga cccgtataat attgcaaaaa
     4561 atatgcgtca acggggggaa tattttcaaa atgtaactcc aggagctgct actcaacgtt
     4621 ttttacaaga aaaaattagt atcatggcag tagttggagc aatttttacg gttattatgg
     4681 gaggtctccc aatgttggct gtggtgggga agacaaacca agttagtatt gcaatgatcg
     4741 tcattaatat ttatattatt tcaactttta tgctcagtat tattgagcaa gttaatacct
     4801 tgaagttgtg gggcagtcac cgaaatatta tttaaggagc aatcatgttt tattttattc
     4861 cttcgtggta caattctgat caaaagtggc ataatgaagc tcctatctgg tatcgtgtat
     4921 tggagaacat gacttttgat gatactgtta gtcaggttag aatgtttcag aatgcagatg
     4981 aggaagtgcg tttacttcta tttagttata atcctcagtt gagatacatg cttcatgaag
     5041 agagtatgtt ggctgtctct tattggtctt tttttgacga tattcaaaat attcaacaaa
     5101 aagagattaa gttaattaat tttaaagaac tgaattggcc taaagggact tattttgttt
     5161 atacaccttt tttagtatta gctaaacatg gtggcgagat tattgcacgt atccaatttg
     5221 cggaaaatgg gaatttacta ctgattgaac ttttaataga tggtgtgctt gataagatta
     5281 tgatatttga cgatcgtggt tttgtatcca gtatcaaata tttcaaacaa aatagattaa
     5341 tttatcaaga ttatttaaat caaaatggtg tttggcaaat tcgagagtat gcttcaaaat
     5401 ttccgagaat agtagttaat ccaatgtctg ataaggtgtt taattttact gagtatgatg
     5461 attgggatac tcttattcga gagagaatat tggttttaaa aaaactagaa gtgactccag
     5521 atgattatat tatagtagca gtaaacgata ttcataacaa tttagtcaga aaatcatttc
     5581 cagatcaaaa gaaaattttc tcattttttg gtgaacgcta ttcatttgat aatcgactcg
     5641 actttaatga acttcttgat gaaggtgaat tgtttattgc tgaaagcgaa aaaatagaac
     5701 ggttattgat tacttcatta caagagaaaa attattcgtc aaactatgtt gagaatcaca
     5761 ttagccgtat tccccctttt gatactcgtt tgagactcgg acagagccaa aatattgaag
     5821 agcttattgc atatttactt gtagatgatc tttctcaaac ttatttaagt gaagttttag
     5881 accaattatt agcaaaaatg gaagttaatc cgtcattaga gttgaaaata gtcagctata
     5941 aaaatgatta tgatatgact tctctaaaag aacaagttaa ggacaaaata ttgtcttcta
     6001 aaaatccaga ttattttttt acctctcttt ctttggaaga tgagaatcat tttaaggaag
     6061 aacaaaaaac taaaattcgt ggcattagtt ttgatatata tacaaatgaa aatcaaatta
     6121 tagatgctct agatactgct aggttagtta ttgatttaag agatgatcca gacctatata
     6181 ctcaaattgc tgctctgagt tctggaattc ctcaaatcaa taaagttcac actaattatg
     6241 tagaacacca gaagaatggc tggattatta gtgactctac agaactcaat aatgcaattg
     6301 actattattt tgatggacta atgaattgga ataaatcgtt agtttacagc gtaaaaaaaa
     6361 tgggtgatta tacaagcggt aagatattac agcgctggcg aaaattatta gaaaaagaat
     6421 aatatggaaa aattaaaaat tttgcagatt ggtgaggaac catgggatgc tgatttaata
     6481 actccagaca atattgaatg gttatactgt aatggaaatg ctattcaaat tttcttagag
     6541 aaattaaaaa gtaaagagtt agaaaaaaat aatgttgaat atattggtaa aacttcaact
     6601 aatgaagtta aattatggtt taatgcggtc attttaacta gtgaggttag cgaattgcaa
     6661 ttgaacctac tttcagattg cttagaagcg catacactct tttacgacga ggattttcaa
     6721 ctcgattttt gctcagaaaa cggtatattt aaacgtaagg ttttgagacc actaccattt
     6781 aatggatcaa ggaaagaaaa aattattttc ctaagccgta ctcttttttc ggaacaatat
     6841 ggtgcaaaat tgaaggtttc tgacatagat ctaaatcctg attttaaggg agagataata
     6901 tatgaaggca atagctctat ttgtttttca gggaattttg gagaagattt tcaacctcta
     6961 ttctcatttc gctataattt atcctctact gataaagcga tagaaatatg gcaagaatat
     7021 caaaaattgc aaggtgataa tagtattatg atagaagtca ttcaatacca aaaaggatca
     7081 cttgatttga tattaaatac aaaatacctt tcagaaaatg atttggctga tccatatatt
     7141 ttagaatatg atgaatcagt tggtttttca tcaatttcta tctttgctag aggtgacgga
     7201 gtggtgagat ttggtgcact acattggcga tattcgcgta aaggtttggg tcaattttta
     7261 atcggtggag aaaggtacag tgattccaaa aggcaagagt ttatctacta ttttaaccct
     7321 ggagatatga aacctccatt gaatgtttat ttttcaggtt ttcgaggagc cgaaggtttt
     7381 gagggttttt acatgatgaa acgtttggga gctccgtttc tcttaattgg tgatccaagg
     7441 attgaaggtg gaagcttcta cctaggtact tcagaatacg aaagtgaatt agaaaatgtt
     7501 ttacaaaaat atttagatta cctacaattt gatcattgtc aacttatttt atcaggttta
     7561 tctatgggaa gttttggtgc cctttattat ggttctactt tttcccccca tgctatagtt
     7621 gtggggaaac cctttacaaa tttaggcgat atggtagtaa atttaaaatt aagaagacca
     7681 gatgattttg aa
//
LOCUS       DATRQA010000096         7542 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.096, whole genome shotgun sequence.
ACCESSION   DATRQA010000096 DATRQA010000000
VERSION     DATRQA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.096"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            231..1160
                     /locus_tag="VBD27_001496"
     CDS             231..1160
                     /locus_tag="VBD27_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO7025499.1"
                     /translation="MNSLDNLLSHNLEDNRHLLPYEHMHTEVRNGYPDILFHWHPELE
                     ISYVHEGTARYHIDYDFFNSQSGDIILIRPNGMHSIHPIENKEHITDSIKFHLDLIGY
                     SIVDQVSLRYLQPLQTSSFKFIQCIKPSMTGYNDIKNCLFDIFNISKEENRHFELLLK
                     AKLNELLYLLYYHQYVIKKHTDDTYRKNERIRDLIDYINNNYQQNLTIEFLADYMGYS
                     KTHFMTVFKQHTGTSCTEFIIQVRLNKASEHLINSTTAIIDIANSVGFNNLSNFNRQF
                     KRYYHTTPRQYRKQFTNKKDGSTLVYRNINTKK"
     gene            complement(1217..2749)
                     /locus_tag="VBD27_001497"
     CDS             complement(1217..2749)
                     /locus_tag="VBD27_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEO7025500.1"
                     /translation="MENHKNEFQFSLESILGFVWRGIVVGLIAGFVVSIFRLAIEKIF
                     LVVMELYKSAHYQPIILLSITVTSIIAAVIIGFFIKSDPDIKGSGIPHVEGELKGMLS
                     PDWFSIVWKKFIAGILAISSGLMLGREGPSIQLGAMTGKGIAQYLNASRMEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLVWITALVASLVANFVSLNIFGLTPVLA
                     LPSELPSLNLNFYWIFLLMGLFLGILGFIYEWVILRFHVIYDYLGKLFHLPSHLYGIL
                     AVIFILPIGYYFPQLLGGGNGLIVSLPRSNLSLMMLGLFFLIRFLWSMLSYSSGLPGG
                     IFLPILALGSLAGAFFAVGMQYFGIISHQQISLFVVLGMAGYFGAISKAPLTAMILVT
                     EMVGDLKQLMAIGIVTMVSYIVMDLLKGEPIYEAMLAKMTFNPKDKVMTPTLIELTVS
                     DKISGKYVRDLELPENVLITTQIHHKTSAVVSGNTILNAGDTIFLVVNESEIKEVREQ
                     LM"
     gene            complement(2858..3931)
                     /locus_tag="VBD27_001498"
     CDS             complement(2858..3931)
                     /locus_tag="VBD27_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7025501.1"
                     /translation="MRRVYSFLGGIILVILILFGLTTYLEKKSSSTPNSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMHTKIKQGGTTYDIAVPSDYMIDKMIKEN
                     LLVKLDHSKIANWDAIGARFKNLSFDPKNKYSIPYFWGTVGIVYNDQLVKTPPKHWDD
                     LWRPEFRNKIMLVDSAREVIGVGLNSLGYGLNTKNISELKAASKKLDALTPNVKAIVA
                     DEMKGYMIQGDAAIGVTFSGEAREMLDGNKHLHYVVPSEGSNLWFDNIVIPKTVKHRK
                     EAYAFINFMMEPKNAAQNAEYIGYATPNLKAKALLPADIKNDKAFYPPDKTIDHLEVY
                     NNLGQKWLGIYNDLYLQFKMYRK"
     gene            complement(3924..4700)
                     /locus_tag="VBD27_001499"
     CDS             complement(3924..4700)
                     /locus_tag="VBD27_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025502.1"
                     /translation="MKKFANIYLAFVFIILYIPIIYLIFYSFNKGGDMNSFTGFTFSH
                     YGELFQDSRLMLILVQTFFLAFLSALLATIIGTFGAIWIYQVRRRHQTSILSLNNILL
                     VAPDVMIGASFLLVFTVIGLQLGFTSVLLSHVAFSIPIVVLMVLPRLKEMNDDMINAS
                     YDLGASTWQMLKEVMLPYLSSGIISGFFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARRGISLEINALSTIVFLFSILLVIGYYYISKEKGEKNA"
     gene            complement(4697..5491)
                     /locus_tag="VBD27_001500"
     CDS             complement(4697..5491)
                     /locus_tag="VBD27_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025503.1"
                     /translation="MKKTSSLFSIPYMAWLFLFVLAPVALIAWNSFFDINGHFTLANY
                     QTFFSSGTYLKMSFNSVLYAGIVSFITLLISYPAAYLLTKLKHKQLWLMLIILPTWIN
                     LLLKAYAFMGIFGQQGGINAFLTFIGIGPKQILFTDFSFLFVAAYIELPFMLLPIFNA
                     LDDIDQNLIYASDDLGANAWQTFQKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLITQNKGMGSTIGVILILVMVAIMWLTKERKA"
     gene            complement(5475..6629)
                     /locus_tag="VBD27_001501"
     CDS             complement(5475..6629)
                     /locus_tag="VBD27_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025504.1"
                     /translation="MTNPIIAFKNVSKVFEDSNTVVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLEASTGDIYLDGKRINDVPTNKRDVHTVFQNYALFPHMTVFENVAF
                     PLKLKKMDKKEIQKRVQETLKMVRLEGFEKRAIQKLSGGQRQRVAIARAIINQPKVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGKMIEDYLVEFNGKRFEAVDGGMRPNESVQV
                     VIRPEDLQITLPDEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIEGEVIG
                     LDFTPEDIHIMRLNETEEEFDARIEEYVDTDDHEDGLINAIEEERNEENL"
     gene            complement(6678..7541)
                     /gene="murB"
                     /locus_tag="VBD27_001502"
     CDS             complement(6678..7541)
                     /gene="murB"
                     /locus_tag="VBD27_001502"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO7025505.1"
                     /translation="MRFDEPLKKYTYTKVGGPADYLAFPRNRLELSRIVKFANSQNIP
                     WMVLGNASNIIVRDGGIRGFVIMFDKLSTVTVNGYVIEAEAGANLIETTRIARYHSLT
                     GFEFACGIPGSVGGAVFMNAGAYGGEIAHILLSAQVLTPQGELKTIEARNMQFGYRHS
                     VIQESGDIVISAKFALKPGDHLMITQEMDRLTYLRELKQPLEYPSCGSVFKRPPGHFA
                     GQLISEAHLKGQRIGGVEVSQKHAGFMVNIAEGSAQDYENLIEHVINTVESTSGVHLE
                     PEVRIIGESLL"
BASE COUNT         2634 a         1467 c         1121 g         2320 t
ORIGIN      
        1 aaatccttac atgagataag gagattaaac gaccctgtaa ggattttgaa gttattagaa
       61 acaccctaag caaaaatttt aaagcgtttt ctctatgact tatattatat gtcatgttcc
      121 tatacaaaac ttacactatc ctttcaaaaa ataatactat cctacgaaac ccattttttg
      181 atagttttct gttataataa tactatccta cgattttaga aagaaaaacg atgaactcac
      241 ttgataattt actatcacat aatttagaag ataaccggca tttattaccc tatgaacaca
      301 tgcatactga ggttagaaat ggttaccctg acattttatt tcactggcat ccggaacttg
      361 aaatttcata tgtccacgag gggaccgcac gttatcacat cgattatgat ttttttaata
      421 gccaatctgg tgatattatc ttgatccgtc caaatggtat gcattctatt catccgattg
      481 aaaataaaga gcatatcaca gatagcatta aatttcactt agatttaata ggatattcta
      541 ttgtagacca agttagcctt cgttatttac aaccgttgca gactagctct tttaagttta
      601 ttcaatgtat caaacctagt atgacaggat ataatgatat caaaaattgt ttgtttgata
      661 tttttaatat ttcaaaagaa gaaaatcgac attttgagct actcctaaaa gctaaattaa
      721 acgaactctt gtatttactc tattatcatc aatatgtcat taaaaaacat actgatgata
      781 cctacagaaa aaacgagaga attcgtgatc tgattgatta tattaataat aattaccagc
      841 aaaatcttac cattgaattt ttagctgatt atatggggta tagtaagaca cattttatga
      901 ccgtatttaa acagcatact ggaacatcct gtacagaatt tatcatacaa gttcgtctta
      961 ataaagctag tgagcatctt attaactcca ctacggccat tattgatatt gccaacagcg
     1021 ttggttttaa taatctatca aattttaatc gccaatttaa acgctattat catacaactc
     1081 ctcgccagta ccgcaaacaa tttaccaaca aaaaagacgg tagtacgctt gtctatagaa
     1141 atataaatac aaaaaaatag aaactagtta aatccatgac tagtttctat tttttattat
     1201 ttaagcgtta attagtttac atcagctgct cacgtacttc tttaatttcc gattcattca
     1261 caactaaaaa aatggtgtcg ccagcattca agatagtatt tcctgatact acagccgatg
     1321 tcttatgatg aatttgagtc gttattagca cattttctgg taattctaaa tctcggacat
     1381 actttcctga tattttatct gaaacagtta attcaatcaa tgtaggtgtc attactttat
     1441 cttttggatt gaaggtcatt ttagccaaca tcgcttcata aataggttct ccctttaata
     1501 aatccatgac aatataagat accattgtta caattccaat agccattaat tgttttaaat
     1561 cgccaaccat ttctgtaacc aaaatcatag ctgttagtgg tgcttttgag attgcaccaa
     1621 aatagcccgc cattcctaga acaacaaata aagatatctg ctgatgagat attataccaa
     1681 agtactgcat acctactgca aaaaaagcac ctgctaaaga tcctaaagct agaattggta
     1741 aaaaaattcc tcctggcaag ccactactgt aactcaacat actccataaa aaacgaatga
     1801 ggaaaaataa tcctaacatc atcagactaa gattactcct aggcagtgat acaattaagc
     1861 catttccacc ccccaataat tgtgggaaat agtaaccaat aggaagaata aaaattaccg
     1921 ctaatatacc atataggtga gatggtagat gaaatagttt acctaaataa tcatagatca
     1981 catgaaatct caaaattacc cactcataga taaagcctaa gatacccaaa aagagaccca
     2041 tgagtaaaaa aatccaataa aagttgagat ttaaagatgg taattctgat ggtaaagcta
     2101 aaactggggt taaaccaaag atattaagtg agacaaagtt tgccactaaa cttgctacca
     2161 aagctgttat ccaaactaag cgagaaaaat gatggtaaat ttcttcaact acaaataaaa
     2221 ggcctgcaat aggagcattg aaggctgctg aaagacctgc tgctgcacca ctagcaatta
     2281 aaacacgttt ttccattcga ctagcattga gatactgtgc aattccctta cctgtcatag
     2341 ctcctaactg aatactggga ccttcgcgac caagcataag tcccgaagaa atagctaaaa
     2401 tacccgcaat gaatttcttc catacaatac taaaccagtc tggcgatagc attcctttaa
     2461 gttcaccttc aacatgcggg atacctgaac ctttaatatc aggatcagat ttgataaaaa
     2521 aacctattat cactgcagcg ataatactag taaccgtaat gctcaataaa ataattggtt
     2581 gataatgggc acttttataa agttccataa caactaaaaa aattttttca atcgctaatc
     2641 gaaatatgct gacaacaaat cctgctatta agcctactac tattcctctc caaacaaatc
     2701 ccagaattga ttcaagtgaa aactgaaatt catttttatg attttccata ttttcctcct
     2761 tttcttacaa ttattttaac aaaaatatca caaactttct aggaactctt ggtaactaaa
     2821 aatacctctc atcagagaag tacttttagt taatagatta cttacgatac attttaaatt
     2881 gtaagtacaa atcattataa ataccaagcc atttttgacc caagttatta taaacttcta
     2941 aatgatcaat tgttttatca ggaggataaa atgctttatc atttttgata tctgctggta
     3001 acagcgcttt tgctttgaga ttaggggtcg cataaccaat atattctgca ttttgtgccg
     3061 catttttagg ttccatcatg aaattaataa aggcataagc ttctttgcga tgctttactg
     3121 ttttggggat tacaatatta tcaaaccaaa ggttcgatcc ttcactagga acaacgtaat
     3181 gcaaatgctt gttcccatca agcatttctc gtgcttctcc agagaatgta acaccaatcg
     3241 ctgcatctcc ctgaatcata tagcctttca tctcatcagc aacaatagcc ttaacgtttg
     3301 gtgttagagc atctaacttt ttagaagcgg cttttaattc tgatatgttc tttgtattca
     3361 atccatatcc caaggaattt aaaccaactc caataacctc acgtgcacta tcaactagca
     3421 tgattttatt tcgaaactct ggacgccata aatcatccca atgtttagga ggagttttaa
     3481 ctaattgatc attataaaca atcccaacag ttccccaaaa ataaggaatc gaatatttat
     3541 ttttaggatc gaaactcaaa tttttaaacc tagctccaat agcatcccaa ttagctatct
     3601 ttgaatgatc aagtttaacc agaaggtttt cttttatcat tttgtcaatc atataatcac
     3661 taggtacagc aatatcatat gtcgtcccac cctgcttaat tttggtgtgc atagcctcat
     3721 tagagtcaaa cgtttcgtat tgtacttcaa ttccagtttc ttttgtaaat ttttttaata
     3781 aggctggatc aatatagtct ccccagttat aaataactaa cttatctgag ttaggtgttg
     3841 aactactctt cttttctaag taagtggtta aaccaaagag gatcaggata acaagaataa
     3901 taccgcctaa aaaggaataa actctacgca ttcttttctc ccttctcttt cgaaatatag
     3961 taataaccaa ttaccaataa aatactaaat aaaaagacaa tagttgacaa ggcattaatt
     4021 tccaatgata taccgcgacg tgcgcgtgaa tagatttcta ctgaaagcgt tgaaaagcca
     4081 ttacctgtaa caaagaaagt aaccgcaaaa tcatctagcg agtaagtgaa agccataaag
     4141 aaacctgaaa taatacccga cgacaaatag ggtaacataa cttctttaag catttgccaa
     4201 gttgaagctc ctaaatcata ggaagcatta atcatatcat cattcatttc ttttaaacga
     4261 ggtaagacca ttaaaacaac aataggaata gagaatgcaa catggctcaa taatacagaa
     4321 gtgaaaccta actgtaaacc aataacagta aatactaaga gaaaacttgc accaatcata
     4381 acatctggtg ctactaaaag gatattatta agcgataaaa tagaggtttg atgtctacgt
     4441 cttacctgat aaatccaaat agctccaaag gtaccaataa ttgtagctaa taaggcgcta
     4501 agaaaagcca aaaagaatgt ttgcactaaa attagcatta agcggctatc ctgaaacagt
     4561 tctccatagt gactaaaggt gaatccagta aaagaattca tatccccacc tttattgaaa
     4621 gaataaaaaa tcaaataaat aataggaata taaaggataa taaaaacaaa tgctaaatag
     4681 atatttgcga attttttcat gctttcctct cctttgttaa ccacataata gcaaccatca
     4741 ctaaaattaa aataacccct atggttgagc ccataccttt attttgtgtg attaaaaaat
     4801 gttgttctat ggctgtccct aaagtaataa ctcgattacc accaataaga cgcgtaagca
     4861 taaagagact taaactagga ataaagacag attgtacacc tgctcttaca ccattcagag
     4921 atagaggaaa tataaccttc tgaaaagttt gccatgcatt tgcacccaag tcatcacttg
     4981 cataaatgag attttgatca atatcatcca aggcattaaa aatgggtaat aacataaacg
     5041 gtaattcaat gtaagctgct acaaaaagaa atgaaaaatc tgtaaataga atctgttttg
     5101 ggccgatacc tataaaggtt aagaaggcgt taattccccc ttgttgacca aatatcccca
     5161 taaaagcata ggcttttaac aaaagattaa tccatgttgg caaaataatt agcattagcc
     5221 ataattgctt gtgttttaat tttgtaagca aataagcagc tggatagcta atcaaaagcg
     5281 ttatgaaact aacgatgcct gcatatagga ccgagttaaa gctcatttta agataggttc
     5341 ctgagctaaa aaacgtctgg taattagcta aagtaaagtg cccattaata tcaaagaacg
     5401 agttccaagc aatcaaagca acaggtgcta atacaaataa gaataaccaa gccatataag
     5461 gaattgaaaa tagactagag gttttcttca tttctctcct cctcgatagc attgattaaa
     5521 ccatcttcat gatcgtcagt gtctacatat tcttcaatgc gcgcatcaaa ttcttcttct
     5581 gtttcattaa gacgcataat atgtatatct tctggagtga aatctaaccc aataacttca
     5641 ccctcaatag ctttacgagt tgaatgaatc atccattcat tacctaaatc atcataagca
     5701 ataatttcat agtggacacc tcgaaaaagt tgtgtgtcca ctttaacttg aagttttcct
     5761 tcatcgggta gggtaatttg aagatcttct ggacgaataa ctacttggac tgattcatta
     5821 gggcgcatac cgccatcaac agcctcaaag cgcttaccgt taaattcaac caaataatcc
     5881 tcaatcatct tgccagataa gatatttgat tcacctataa aagttgctac aaaatgattg
     5941 ataggttcat cgtagatatc tactggtgtt cctgattgaa caatttcacc ttcattcatg
     6001 acgaaaatcc aatcactcat tgccaacgct tcttcttgat catgtgtgac aaaaacaaag
     6061 gtaattccta aacgttgttg cagttcgcgc aattcatatt gcatctctgt acgcaatttc
     6121 aaatccaatg ctgataaggg ttcatctagt aagactactt taggttgatt gatgatagca
     6181 cgcgctatcg caacacgctg gcgttgtcct cctgataatt tttgaatagc tcgcttttca
     6241 aacccttcta aacgaaccat ttttagagtt tcttgaacac gtttttgaat ttctttctta
     6301 tccatttttt tcaactttaa tggaaaagca acgttttcaa aaaccgtcat atgtggaaac
     6361 aaagcatagt tttggaaaac ggtatgaaca tcccgtttat tagtgggaac atcattgatg
     6421 cgtttcccat ctaagtaaat atcgcctgta ctcgcctcta gcaaacctgc aataatattt
     6481 aagatagttg atttaccact tcctgaggcc cctaataagg tgtaaaattt accttcttca
     6541 agttcaaaat taatatcttt taagacaacc gtgttgctgt cttcgaatac ctttgataca
     6601 tttttaaatg caatgattgg gttagtcaat tctcataaat cctccatttt tctgactaaa
     6661 gtaagtacta aactacttta taatagcgac tcaccgataa tacgaacttc tggttccaaa
     6721 tggacacctg aagttgattc aacggtgtta ataacatgtt caataagatt ttcataatct
     6781 tgagctgaac cttctgcaat gttaaccata aaaccagcat gtttttgact aacctctact
     6841 ccaccaatac gttgaccttt aagatgtgct tcgctaatga gttgccctgc aaaatgtcct
     6901 ggaggacgtt tgaacacaga accacaagaa gggtattcta aaggttgttt cagctcacgg
     6961 agataggtta aacgatccat ttcctgagta atcatgagat gatctcctgg ttttaaggca
     7021 aacttagctg atataacaat atctccactc tcttgaatga cagaatgacg ataaccaaat
     7081 tgcatattcc tagcttcaat cgttttgagt tcaccctgtg gtgtcaaaac ttgtgcagac
     7141 aataaaatat gagcaatttc accaccgtaa gcgccagcat tcataaagac tgctccacct
     7201 acacttccag gaattccgca agcaaactca aagccagtta aactatgata acgtgcaata
     7261 cgagttgttt caatgagatt agctcctgct tctgcttcaa taacataacc gttgactgta
     7321 acagttgaca gtttatcaaa catgataaca aaaccacgga taccaccatc tctaacgata
     7381 atattactcg catttcctaa aaccatccaa ggaatatttt gactgttagc aaacttaaca
     7441 atacgcgata gttcaaggcg attccttggg aatgcaagat aatcagcagg tcctcctact
     7501 ttagtataag tatatttctt taaaggctca tcaaagcgaa ta
//
LOCUS       DATRQA010000097         7463 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.097, whole genome shotgun sequence.
ACCESSION   DATRQA010000097 DATRQA010000000
VERSION     DATRQA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.097"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            157..774
                     /locus_tag="VBD27_001503"
     CDS             157..774
                     /locus_tag="VBD27_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025506.1"
                     /translation="MRKFFQDVIVEVAYYLEIALSVILATAIIFFSLALFHDLFSMLS
                     VDQNVDKLFQTFLARAMTIAVGIELIKMFSKPSPDTLIEVLLFALARQLVIEHGSVSD
                     YLMGIASVAILFAVRKYLFTHFDESSNIVLRANNKVKKANILARVQLPCQPNETLGDL
                     LVRHLNDEEKDVAIGSVVYINNVALRVDSMHDGLVTRVEIIRSLH"
     gene            942..2381
                     /locus_tag="VBD27_001504"
     CDS             942..2381
                     /locus_tag="VBD27_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7025507.1"
                     /translation="MENFLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPLHDVFAG
                     FIKATVGYLILNVGAGGLVNTFRPILVALAKKFHLEAAVIDPYFGLASANAKLETMGF
                     ISVATTALLIGFGINILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQLRNG
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGGILAVLGPDIMSNVKLIGPGAFVPTKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVTELTNAFQGISNKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVVFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLAGGYHGNIDFEFPWLAFGYIFKYLGIAGYVIV
                     CLFFLAIPQLQFMKSKDKEAYYRGDAPAE"
     gene            2409..2687
                     /locus_tag="VBD27_001505"
     CDS             2409..2687
                     /locus_tag="VBD27_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7025508.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVSNFESASCSVGEA
                     KGLAANYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEIL"
     gene            2754..3239
                     /locus_tag="VBD27_001506"
     CDS             2754..3239
                     /locus_tag="VBD27_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7025509.1"
                     /translation="MNLKQAFIENDSIRLKLSASDWKEAIKLSIDPLIESGAVDAEYY
                     DAIIESTEEFGPYYILMPGMAMPHARPEAGVKRDAFSLITLTEPVVFPDGKEVSVLLA
                     LAATSSAIHTSVAIPQIIALFELENSIQRLTECQEAKEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            3352..4017
                     /locus_tag="VBD27_001507"
     CDS             3352..4017
                     /locus_tag="VBD27_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEO7025510.1"
                     /translation="MTKRLPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKEERGDRGEIQIELYGDWTYEQAQQWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLSTDTLQLFEGVDVFTFIAGRGITEADDPAAAARAFKDEIKR
                     IWG"
     gene            4021..4884
                     /locus_tag="VBD27_001508"
     CDS             4021..4884
                     /locus_tag="VBD27_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEO7025511.1"
                     /translation="MTRPIGIYEKATPKHFNWLERLQFAKELGFDFVELSIDESDERL
                     ARLEWSKEERLELVKAIFETGVRVPTITFSGHRRFPMGSNDPEKEARAMDMMKKCIVF
                     AQDIGIRNIQLAGYDVYYEEKSPETRARFIKNLRQACTWAEEAQVILSIEIMDDPFMN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDLWSEFYNGHRSIAALHIKDTYAVTET
                     SKGQFRDVPFGQGCVDWEEMFAVIKKTNYNGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLMEKAGVK"
     gene            4886..5602
                     /locus_tag="VBD27_001509"
     CDS             4886..5602
                     /locus_tag="VBD27_001509"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO7025512.1"
                     /translation="MAKSLQEMRERVCEANKSLPVHGLVKFTWGNVSEVDREAGLIVI
                     KPSGVDYDQLTPENMVVTDLEGNIVEGDLNPSSDLPTHVQLYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLSEDEVNTAYEKETGSVIIEEFERRNL
                     DPMAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVAKMNRFTEQINPRVEPAPKYIMD
                     KHYLRKHGPNAYYGQKGQEH"
     gene            5613..6260
                     /gene="fsa"
                     /locus_tag="VBD27_001510"
     CDS             5613..6260
                     /gene="fsa"
                     /locus_tag="VBD27_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO7025513.1"
                     /translation="MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVIN
                     EICQIVDGPVSAEVTGLTCDEMVTEAREIAKWSPNVVVKIPMTEEGLAAVSQLSKEGI
                     KTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFYGFQSEI
                     IAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK"
     gene            6423..7406
                     /locus_tag="VBD27_001511"
     CDS             6423..7406
                     /locus_tag="VBD27_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7025514.1"
                     /translation="MFEKVTISDIANLVGVSKATVSYYLNGNYKKMSLQTKEKIRLAI
                     KETGYQPSKIAQSLVTKNTRTIGVVIADITNPFISSVMKGIHDTCQQFGYSVNFTNSD
                     NDIDIELENLNRLNQQNVSGIILDSVDPNHSFIETLSNDRLVMVDRQAKDIKVDTVVS
                     DNKESTQIFLEKMQETGYHDIYFVTYPIEGISTRELRYEGFKEVVSSNPDKLIIITED
                     GSTQRILDIIEHSEQKPGFLMMNGPTLLNFMKKLNQSTVSYPEDYGLGSYEDLEWMQV
                     LTPNVSCIKQDSYGIGCLAAQCLIEKISQGNEPTTARLLEVKNQIVIRQSF"
BASE COUNT         2316 a         1209 c         1522 g         2416 t
ORIGIN      
        1 atttaccaat aagcttatta tatcataatg tataatcaaa cattcgttag tactgattaa
       61 aaggtttatg gaggggcggt tactattgtt tcattgaatt agtaacttta acgacgaaat
      121 gttataatgt tattagttta taaaagagga gattgtatgc gtaaattttt ccaagatgtt
      181 attgtagagg tggcttatta tttagagatt gctttgtcag ttattctggc aacagctatt
      241 attttcttta gtttagcact ctttcatgat ttgtttagca tgttgtctgt tgatcaaaat
      301 gttgataagt tgttccagac tttccttgct cgcgccatga caatagcagt cgggattgag
      361 ttgatcaaaa tgttttcaaa accttcccct gatactttaa ttgaagtgtt attgtttgcc
      421 ttagctcgtc aacttgtaat tgaacatggt agtgtttcag attaccttat ggggattgct
      481 tcagttgcta ttttgtttgc tgttcgtaaa tatcttttca cccattttga tgagtcaagc
      541 aacattgttt tacgtgctaa taacaaagtc aaaaaagcta atattttagc tagggttcaa
      601 ctaccttgtc aacccaatga aactttagga gacttacttg ttcgtcattt aaatgatgag
      661 gaaaaggacg ttgctattgg ttcagtagtc tatattaata acgttgcatt gcgtgttgat
      721 agtatgcacg atggtcttgt gacgcgtgta gaaatcatta ggagtctaca ttaaaaaata
      781 taaaaaatca tgtatcttag gatacatgat ttttttgtaa attaggttca gcacaatttt
      841 gcacacttaa gtgtgtactt cttggtcttt tttaaaacgc ttacaaaaaa tataataaag
      901 atacaaaaaa acattactta aataaagaga ggtctttagt tatggaaaac ttcctagctc
      961 cccttaattg gttctcgcaa aatatcttgc agaatccagc cttcttcgta ggtctgttgg
     1021 tactcattgg gtatctttta ttgaagaaac cacttcatga tgtctttgct ggttttatca
     1081 aagcgaccgt tggatatttg attttgaacg taggtgcagg aggattggtt aatacattcc
     1141 gtccaatctt agttgctctt gctaaaaaat ttcatttaga agctgctgtt attgaccctt
     1201 actttggttt agcatcagca aatgctaagt tagaaacaat gggctttatc agtgttgcaa
     1261 caacagcact cttgattggt tttgggatta acattctctt ggttgctctt cgtaaagtaa
     1321 ctaaagtccg tactcttttt attactggtc atatcatggt acaacaagct gcaacaattt
     1381 cagtatttgt actgctcttg attccacaac ttcgtaatgg ttttggtgca tgggctgttg
     1441 gtatcatttg tggtctatat tgggcggtaa gttctaatat gaccgttgaa gcaactcaac
     1501 gccttacagg tggtggcgga tttgccatcg gacatcaaca acaatttgca atttggtttg
     1561 ttgataaagt tgcgccattt ttcggtaaaa aagaagaaaa tcttgataac ttaaaattac
     1621 caacattctt aaatattttc catgatacag ttgttgcatc agcaacattg atgctagttt
     1681 tctttggagg aatcttagct gtccttggtc cagacattat gtctaatgtt aaattaatcg
     1741 gacctggtgc atttgtacca actaagcaag ctttcttcat gtacatcctt caaacatcat
     1801 tgacattctc agtttacctt ttcattttga tgcaaggtgt acgcatgttc gttacagaat
     1861 tgacaaatgc cttccaaggt atctcaaaca aacttcttcc aggatcattc ccagcggttg
     1921 acgttgcagc ttcatatggt tttggttctt caaatgcagt tctttcagga tttgcttttg
     1981 gtttgattgg tcaattaatt acaatcgctc tacttgttgt cttcaaaaat ccaatcttga
     2041 ttatcacagg attcgttcca gtattctttg ataatgcggc aatcgctgtt tatgcagata
     2101 aacgtggtgg ctggaaagca gcagtagctc tatcatttat ctcaggtatt attcaggtgg
     2161 cacttggtgc tgttgctgta ggtcttttag gacttgcagg tggttatcat ggaaatatcg
     2221 actttgaatt cccatggtta gcatttggtt acatcttcaa gtatttaggt attgctggtt
     2281 acgtgattgt atgtctcttc ttcttagcta ttccacaact tcaatttatg aaatctaaag
     2341 ataaagaagc atactatcgt ggtgatgcgc ctgcagaata atagaaattt ataacggagg
     2401 aaataaaaat ggttaaagtt cttacagcat gcggaaacgg catgggatca tcaatggtta
     2461 tcaaaatgaa agttgaaaat gcgcttcgtc aattaggcgt tagcaacttt gagtcagcat
     2521 cttgctcagt cggagaagca aaaggtttag ctgcgaacta tgatatcgtg gttgcttcaa
     2581 accaccttat ccatgaatta gacggacgta caaaaggtca tttagtaggt cttgacaacc
     2641 ttatggatga caatgaaata aaaacaaaac tacaagaaat tttgtaagtt aatagacaaa
     2701 gggagtgaga caaaagtttc tctcccatat ctttagactt ttaaggagaa atcatgaatt
     2761 taaaacaagc ctttatcgaa aatgactcaa ttcgtcttaa attatctgca agcgattgga
     2821 aagaagcaat caaattatca attgatccat taattgaaag tggtgctgtt gatgcagaat
     2881 attacgatgc cattattgaa tctacagagg aatttggtcc gtactacatt cttatgccgg
     2941 gtatggcgat gcctcatgct cgtccagaag caggcgtaaa acgtgatgca ttttcattaa
     3001 ttacattaac agagccagta gtctttccag atggcaaaga agtctcagta ttgttagcac
     3061 ttgctgcaac aagttcagct attcatactt ctgttgctat ccctcaaatt attgcccttt
     3121 ttgaattgga aaattcaatt cagcgcttga cggaatgcca agaagcaaaa gaagtccttg
     3181 caatggtaga ggaatcaaaa aacagcccat atttagaagg tttagattta gaaagctaat
     3241 caataaactg atattaatag aaaggatagg acttataaaa aatctctgtg ataagaaact
     3301 ctttgccaca aataaagtga gtatttttat caggtcctta gaataaacaa catgacaaaa
     3361 cgtttaccaa atttacaagt tgcacttgac cactcagatt tacaaggagc tatcaaagct
     3421 gccgtatcag taggacatga agttgatgtt attgaagctg gaactgtttg ccttcttcaa
     3481 gtaggtagtg aattagttga ggtccttcgc agtctcttcc ctgacaaaat aattgttgct
     3541 gatacaaaat gtgctgatgc tggtgggact gttgctaaaa ataatgctgt tcgcggtgca
     3601 gactggatga cttgtatctg ttgtgctact attccaacaa tggaagcagc cttaaaagct
     3661 atcaaagaag aacgcggcga tcgtggagaa attcaaatcg aactatatgg tgattggact
     3721 tatgagcaag cgcaacaatg gttggatgca ggtatttcac aagccattta tcaccaatct
     3781 cgtgatgcgc ttcttgctgg cgaaacatgg ggagaaaaag atcttaataa ggttaaaaaa
     3841 ttgattgata tgggcttccg agtttctgta actggtggtt tatcaactga tactcttcaa
     3901 ttatttgaag gtgttgatgt ctttactttt attgcgggtc gtggtattac agaggcagat
     3961 gatccagccg cagcagcacg tgcatttaag gatgaaatta aacgtatttg ggggtaatgt
     4021 atgacacgtc caataggaat ttacgagaag gcaacaccaa aacattttaa ctggttagaa
     4081 cgcctacaat ttgctaaaga attgggattt gattttgtag aattatccat tgatgagtca
     4141 gatgaacgtt tggcacgttt ggagtggtca aaagaagaac gccttgagtt ggtcaaagct
     4201 atttttgaga caggagttcg tgttccaaca attacattta gtggtcatcg tcgtttccca
     4261 atgggatcta atgatcctga gaaagaagcg cgtgcgatgg atatgatgaa aaaatgtatc
     4321 gtctttgctc aagatattgg tattcgtaat attcaattag caggatatga tgtgtactat
     4381 gaagaaaaat ctccagagac acgtgcacgc tttattaaga atcttcgtca agcttgtact
     4441 tgggccgaag aagcacaagt tattctgtct attgagatta tggatgaccc atttatgaat
     4501 tccattgaga aatatttggc tgtggaaaaa gagattgatt ctccttatct ctttgtctac
     4561 ccagatactg gaaatgtatc agcatggcat aatgatcttt ggagtgaatt ttataatgga
     4621 catcgctcaa ttgcagcgct tcatattaaa gatacctatg ctgttaccga aacgtctaaa
     4681 ggtcaattcc gtgatgttcc atttggtcaa gggtgtgttg attgggaaga aatgtttgct
     4741 gtcattaaga aaacaaatta taatggtcca ttcttgattg aaatgtggtc ggagaactgt
     4801 gagactgtag aggaaacacg tgcagccatt aaagaggcgc aagattttct ttacccatta
     4861 atggagaaag caggagtgaa ataatatggc aaaatcattg caagaaatgc gtgaacgcgt
     4921 gtgtgaagct aataaatcac taccagttca tggtttagtt aaatttactt ggggtaacgt
     4981 ttctgaagtt gaccgcgaag caggattgat tgttatcaaa ccttcaggag ttgactatga
     5041 ccaattgaca cctgaaaata tggttgtcac agacttagag ggaaatatag tggaagggga
     5101 tttgaatcca tcatctgatc ttccaactca tgttcaactt tataaagcat ggccagaagt
     5161 cggtggtatt gttcatactc attcaacaga agcagttggc tgggcacaag cgggtcgcga
     5221 tattccattt tatggaacaa cacatgcaga ttatttttat gggccagtac catgtgctag
     5281 gtcacttagt gaagatgaag ttaacactgc ttatgaaaaa gaaacaggta gtgttattat
     5341 agaggaattt gaacgtcgga atttagatcc aatggctgtt cctggtattg ttgtacgtaa
     5401 tcatggacca tttacttggg ggaaagatcc agcacaagca gtttatcact cagtcgttct
     5461 tgaagaagtt gccaagatga atcgtttcac tgaacaaatt aatccacgtg ttgaaccagc
     5521 tccaaaatat attatggata aacactattt acgtaagcat ggaccaaatg cttattatgg
     5581 tcaaaaagga caagaacatt aaggagaata atatgaaata ctttttagat actgccgatg
     5641 tttctgaaat tcgtcgtctt aatcgtttag gaatcgttga tggtgttact accaatccta
     5701 ctattatttc acgtgagggg cgtgacttta aagaagttat taacgaaatt tgccaaatcg
     5761 ttgatggtcc agttagtgca gaagtgacag gtttaacttg tgatgaaatg gttaccgaag
     5821 ctagagaaat cgctaagtgg tcaccaaatg ttgttgttaa aattccaatg actgaagaag
     5881 gtttagcagc agtttcacaa ttatcaaaag aaggtataaa gacaaatgtg acattaatat
     5941 ttactgtggc tcaagggctt tcggcaatga aagctggtgc aacctttatt agtccttttg
     6001 tgggtcgtct tgaagatatt ggaacagatg cttatgcttt gattcgtgac cttcgtcaca
     6061 ttattgattt ttatggtttc caatctgaga ttattgctgc tagcattcgt ggattggcgc
     6121 atgtggaggg cgtcgctaaa tgcggtgcgc acattgccac tattccagat aaaacatttg
     6181 catcactatt tactcacccg cttactgata aagggattga aactttctta aaagattggg
     6241 atagtttcaa gaagaagtag tgttacgata aagcaaactc ttaattatac tagtgaaaga
     6301 caaactttga taatgggttt gtctttttta taatcttctc caataagaac acttattttc
     6361 atttttctta ttaaatgata taatacggat aaattaagtc tacttagaat aaggaaatag
     6421 agatgtttga gaaggtaacc attagtgata ttgcaaattt agtaggtgtg tcaaaagcta
     6481 cagtttccta ttatctcaat gggaactata agaaaatgtc tctgcaaacg aaagaaaaaa
     6541 tccgtttagc tattaaggag actggttatc aaccaagtaa gattgctcaa agtttagtta
     6601 caaaaaatac ccgtactatt ggggtcgtta ttgctgatat taccaatcca tttatttcgt
     6661 ctgtgatgaa gggaatacat gatacttgtc aacaatttgg ttactcggtt aactttacta
     6721 actcggataa tgatattgac attgagttgg aaaatttaaa ccgtttaaat cagcaaaacg
     6781 tttcaggaat tattttagat tctgtagatc ctaatcattc atttatagaa actttgagta
     6841 atgatcgtct tgtcatggtt gatagacaag ctaaagatat taaggtagat actgtagtat
     6901 ccgacaataa agaatcgact caaatatttc ttgaaaaaat gcaagaaact gggtatcatg
     6961 acatctattt tgtcacttat cccattgaag ggatttcaac ccgtgagctt cgttacgaag
     7021 gttttaaaga agtggtcagt tcaaatcctg ataagttgat tattatcact gaagatggta
     7081 gcacacagag aattcttgat attattgaac attcagagca aaaaccaggg ttcttaatga
     7141 tgaacggtcc aactcttttg aattttatga aaaaattaaa tcaatcaacg gtcagctacc
     7201 ctgaggatta tggtcttggg agttacgaag atttagaatg gatgcaggtg cttacaccaa
     7261 acgtttcctg tataaaacag gattcatatg gcattggttg tctggctgcc caatgtttaa
     7321 ttgaaaaaat tagtcaagga aacgaaccaa ctactgcaag gttactagaa gtaaaaaatc
     7381 aaatcgttat tcgtcaatct ttttaaaaat gtttgcgaga gcaagcattt ttttataaaa
     7441 aagcaaaaaa cactatgtaa acg
//
LOCUS       DATRQA010000098         7329 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.098, whole genome shotgun sequence.
ACCESSION   DATRQA010000098 DATRQA010000000
VERSION     DATRQA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.098"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            23..724
                     /locus_tag="VBD27_001512"
     CDS             23..724
                     /locus_tag="VBD27_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEO7025515.1"
                     /translation="MRVITVKNDIEGGKIAFTLLEEKMKTGAQTLGLATGSSPITFYE
                     EIVKSNLDFSNMVSINLDEYVGIAASNDQSYSYFMHKHLFDAKPFKENNLPNGLAKDL
                     KKEIKRYDAVINANPIDFQILGIGRNGHIGFNEPGTPFDITTHVVDLAPSTIEANSRF
                     FNSIDDVPKQALSMGIGSIMKSKTIILVAYGIEKAEAIASMIKGPITEDMPASILQKH
                     DDVVIIVDEAAASKL"
     gene            complement(796..1752)
                     /locus_tag="VBD27_001513"
     CDS             complement(796..1752)
                     /locus_tag="VBD27_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO7025516.1"
                     /translation="MGLLVDGKWVDQWYDTASTGGKFVRTVTQFRHWVTKDGSAGPSG
                     DAGFKAESGRYHLYVSLACPWASRVLIMRKLKNLESHISISIVNPLMLENGWTFQEYK
                     GVIPDMINQSQYLYQIYQASQSDYTGRVTVPVLWDKKCHTIVNNESSEIMRMLNTAFN
                     HITGNTDDYYPDSLQGQIDEMNNFIYPKINNGVYKAGFATSQNVYQKEVETLFTALDQ
                     LEKHLSDNHYLVGEQFTEADIRLFTTLVRFDTVYYGHFKCNLKALHDYPHLWHYTKRI
                     YNLPGIAETINFDHIKKHYYGSHKTINPTGIIPAGPNLDWTI"
     gene            1848..2567
                     /locus_tag="VBD27_001514"
     CDS             1848..2567
                     /locus_tag="VBD27_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7025517.1"
                     /translation="MRLDKLLGQAGFGSRNQVKKLIRSRQVSVDGQIVTKDNVIVDSG
                     LQSIFVGKERVCLKESSYYLLYKPSGVVSAVRDSEHKTVIDLISEKDKVEGLYPIGRL
                     DRDTEGLLIITNNGPLGYRMLHPKHHVAKTYYVEVNGFLERDAITFFEEGVVFDDGTK
                     CKPAELTIDTANNDKSTARITITEGKFHQVKKMFLAYGVKVIYLRRISFGDLRLDMNL
                     KPGQYRRLRDSEAAILKRYLD"
     gene            2640..3791
                     /locus_tag="VBD27_001515"
     CDS             2640..3791
                     /locus_tag="VBD27_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7025518.1"
                     /translation="MNSKLSLKLTILSISIFLMSHLAIAPAIPKLYAFYHTQNPHIGL
                     ASVESLVTIPAMMITIFVILSNFVVTKLGKKNTVLLGLCLILMSGFISFFTSNFSLAM
                     ASRLLLGIGIGLYNSLSISIITDLYEADERASMIGLRTASLNIGKALTTFIVGLVLAI
                     GVNYIYLVYLLVIPVFFFFWKNVPEVENQTHTLKASTTFDTKAALLMLITFLVGIAYI
                     GATVKIPTLLVTKYHYATSFSSNMLTLLAFSGILVGSVFGKLVKAFQEKTLLIMILAM
                     GIGNVLFALANNQIIFIVASILIGASFVGTMSSVFFYISKNYAKEHNNFITSLALTAG
                     NIGVILTPLILTKLPSQLHLEPFITPFLITSGLMVINVFVYLVLMSKNK"
     gene            3843..4790
                     /locus_tag="VBD27_001516"
     CDS             3843..4790
                     /locus_tag="VBD27_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO7025519.1"
                     /translation="MLIAKDKQGNLINLLESHPGKGQYFCPTCCSAVRLKAGRIMRRH
                     FAHISLKNCQFHHENESNEHLQLKAKLYMSLSRENETMLEHHLPEINQIADLFVNETL
                     ALEVQCSRLSEQRLRERTKAYLQADFQVRWLLGEKLWLKHRLTNLHKQFLQFSQSIGF
                     HIWELDLRLEVLRLKYLIYEDLRGHVYYLSKTCPLSGDVLAFLKWPYQSKNLNFYKVK
                     QDRNIRDYVRQQLRYGNQFWLRKQEKAYLSGQNLLTQELMMFFPQIQPPRVDTDFCQI
                     TNSLTSFYQNFTNYYQKNKNNLDQTLYPPVFYDKIVTKN"
     gene            4806..6611
                     /gene="pepF"
                     /locus_tag="VBD27_001517"
     CDS             4806..6611
                     /gene="pepF"
                     /locus_tag="VBD27_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO7025520.1"
                     /translation="MSDNRSHIEEKYQWDLTTVFATDELWETEVVELTQAIDDAKGFS
                     GHLLDSSQSLLEITEVELELSRRLEKVYVYASMKNDQDTTVAKYQEFQAKATALYAKF
                     SETFSFYEPELLQLSESDYQSFLLEMPDLQKYDHFFEKIFANKPHVLSQNEEELLAGA
                     SEIFGAAGETFEILDNADMVFPVVKNAKGEEVELTHGNFISLMESSDRTVRKEAYQAM
                     YSTYEQFQHTYAKTLQTNVKSQNFKARVHHYQSARQSALSANFIPEEVYETLIKTVNH
                     HLPLLHRYMKLRQKVLGLDDLKMYDVYTPLSQMDMSFTYDEALKKSEKVLAIFGEAYS
                     ERVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRENQPYVYGDYSIFLAEIASTTNENILTETLLKEVKDDKNRFAILNHYLDGFK
                     GTIFRQTQFAEFEHAIHVADQEGQVLTSEYLNNLYAELNEKYYGLTKEDNHFIQYEWA
                     RIPHFYYNYYVFQYATGFAAANYLAERIVNGNPEDKEAYLNYLKAGNSDYPLNVIAKA
                     GVDMTSADYLDAAFRVFEERLVELENLVAKGVHND"
     gene            6806..>7329
                     /locus_tag="VBD27_001518"
     CDS             6806..>7329
                     /locus_tag="VBD27_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO7025521.1"
                     /translation="MKKLTFIWDLDGTLIDSYVPIMEALEETYRHFGLIFDKELIHEY
                     ILQESVGQLLVNLSEEEQIPHEKLKAYFTKEQESRDSKIHLMPYAKEILEWTKEQDIP
                     NFMYTHKGASTHSVLETLQISHYFDEILTGVSGFERKPHPQGINYLVKRYSLDKSMTY
                     YIGDRPLDLEVAQNA"
BASE COUNT         2458 a         1139 c         1377 g         2355 t
ORIGIN      
        1 accaaataaa aggagagtaa acatgagagt tattacagtt aagaacgaca ttgagggggg
       61 aaaaattgca tttactcttt tagaagagaa aatgaaaact ggtgctcaaa ctttaggatt
      121 ggcaacagga agtagcccaa tcacctttta cgaagagatt gtaaaaagta accttgattt
      181 ttcaaatatg gtttctatta accttgatga atatgttgga attgccgcaa gtaatgatca
      241 aagttactct tattttatgc ataaacatct ttttgatgct aaacctttta aggaaaataa
      301 ccttccaaac ggcttggcta aagatttgaa aaaagaaata aaacgatacg atgctgtcat
      361 aaatgctaat ccaattgatt ttcaaattct tggaattggt cgtaacggtc atattggttt
      421 taatgaacca ggtacacctt ttgatattac aacacatgtg gttgacttag ctccaagtac
      481 aattgaagct aatagtcgtt tctttaatag tattgatgat gtaccaaagc aagccttatc
      541 aatgggtata ggttctatta tgaaatcaaa aacaatcata ttagtggcgt atggtattga
      601 aaaagcagaa gcgattgcaa gtatgattaa aggacctatc acagaagata tgccagcaag
      661 tatcctccaa aaacatgatg atgttgtgat tattgtggat gaagcagcgg catcaaagtt
      721 gtaaagtaaa taataaaaaa gtaaggatga atctcaatga gaaaaaattc attcttactt
      781 ttttattatg ctttattaaa ttgtccagtc taagttaggt ccagctggaa taataccggt
      841 agggttaatg gttttatggc taccgtaata gtgttttttg atatgatcaa aattaattgt
      901 ctcagcaatt ccaggtaggt tgtaaatacg ttttgtataa tgccataaat gtgggtaatc
      961 atgtagcgct tttaaattac atttaaaatg accataataa acggtgtcaa agcgaactaa
     1021 agttgtgaaa aggcgaatat cagcttcggt aaattgttct ccaacaagat aatggttatc
     1081 agataagtgt ttttctaact gatctaaagc tgtgaataag gtttcaactt ccttttgata
     1141 aacgttctga gaagtagcaa aaccagcttt atagacacca ttatttattt ttggataaat
     1201 aaaattattc atttcgtcaa tttgtccttg taaggaatcg ggatagtaat cgtcagtatt
     1261 tccagtaata tgattaaatg cagtatttaa catgcgcata atttcagatg attcattgtt
     1321 aacgattgta tggcattttt tatcccaaag aacagggact gtcacgcgtc ctgtataatc
     1381 actttgactt gcttgataga tttggtaaag gtattggctt tggttaatca tatcaggaat
     1441 aacgccttta tattcttgaa aagtccaacc attctccaac attaaagggt taacaatgga
     1501 gatagagata tgagattcta gatttttaag ctttctcatg attaatacac gtgatgccca
     1561 agggcaggct agtgaaacgt agagatgata tcgtccagat tctgctttaa aacctgcatc
     1621 cccactaggc cctgctgaac catctttagt aacccaatga cggaattgtg taacggttcg
     1681 gacgaattta ccaccagttg atgcagtatc ataccattga tcaacccatt tgccatctac
     1741 taaaagtccc atgataatct cctttgtttt tctattacta attagtagta taaagatttt
     1801 aaaaggaatt agcaaatgtt taacttacag tatggtaaac taagggtatg cgtttagata
     1861 aattattagg tcaggcagga tttgggtcta gaaatcaggt taagaaatta attcgttcta
     1921 ggcaagtttc tgtagatggt caaattgtta caaaggataa tgttattgta gatagtggtc
     1981 tacaatcgat ttttgtagga aaagagagag tctgcctaaa agaaagttct tattaccttc
     2041 tttataagcc atcaggagtt gtttcagcag ttagagattc agaacataaa acagtcatag
     2101 acttaattag cgaaaaagat aaggtggagg gattgtatcc gattggtcgt ctagatcgtg
     2161 atacggaagg gcttttaata atcacgaata atggtccact gggttaccga atgttacatc
     2221 caaaacacca cgttgcaaaa acgtattatg ttgaagtaaa tggtttcttg gaaagagatg
     2281 ctattacctt ttttgaagaa ggagttgtat ttgacgatgg aacaaagtgt aaaccagctg
     2341 aattaacgat tgatacggct aataatgaca aaagtaccgc aagaataact atcacagaag
     2401 ggaaatttca ccaagtaaaa aagatgtttc ttgcatatgg cgttaaagtt atatacttga
     2461 ggcgtatttc ttttggtgac ttgaggcttg atatgaattt gaaaccaggt cagtatcgta
     2521 gattgagaga ttcagaggca gctatcttaa aaaggtattt agattagtga aacgactcac
     2581 ttgaccttgc ttagtattct tcatatactt ataagtgatt attttaaagg agaaaactat
     2641 tgaattcaaa actttcattg aaattgacta ttttatcgat ttctatcttt ttgatgtctc
     2701 acttagcaat tgctcctgca attcctaaat tgtatgcctt ttaccatact caaaaccctc
     2761 atattggact agcttcagta gagagtttag ttactattcc agctatgatg attactattt
     2821 ttgttatttt gagtaatttt gtagtcacaa aacttggcaa aaaaaacacg gtcttacttg
     2881 gtttatgttt aatcttaatg tctgggttta tctcattttt tacaagtaat ttttcacttg
     2941 cgatggctag tcgactactt ttgggtattg gtattggtct ctataattcg ctatcaatta
     3001 gtatcataac ggatttatat gaggctgatg agagagccag tatgattggt cttagaacag
     3061 catcacttaa tatcggtaaa gccttaacta catttattgt tggattggtt ttagctattg
     3121 gtgtgaatta tatttatttg gtttaccttc tggttattcc tgttttcttt ttcttttgga
     3181 aaaatgttcc agaggttgaa aatcaaacac atacccttaa agctagtaca acatttgata
     3241 ctaaagcggc tttgttgatg ctcatcacgt tcttagtagg tattgcttat ataggagcaa
     3301 ctgtcaaaat tccaacctta ctagtgacaa aatatcacta cgcgacgagc ttctctagta
     3361 atatgttaac tctccttgct tttagtggta ttttagttgg atctgtattt ggaaaattag
     3421 tcaaagcatt ccaagagaag acgctattaa tcatgattct agctatggga ataggtaatg
     3481 tgctatttgc tttagctaat aatcagatca tttttatcgt agctagtatt ttaataggag
     3541 ctagttttgt tggcaccatg tcttctgtat tcttttatat ttcaaaaaac tatgcaaaag
     3601 aacataataa ctttatcaca agccttgcct taacagctgg taatatagga gttattttga
     3661 caccgcttat tttaacgaaa cttccaagcc agctacatct ggaacctttt ataacaccat
     3721 ttttaataac aagtgggctg atggtgatta atgtctttgt ttacctagtt ttaatgtcaa
     3781 aaaataaata aaaagttatc cgaaaggata gcttttttcg aatagtaaaa gtaggagaaa
     3841 aaatgttaat tgctaaagac aaacaaggaa atttaattaa tctactcgag tctcatccgg
     3901 gaaagggaca gtatttttgt ccaacttgtt gttcagctgt aaggttaaaa gcaggtcgaa
     3961 tcatgagaag acactttgca catattagtc taaaaaattg tcagtttcat catgaaaatg
     4021 agtctaatga gcacttgcaa ttaaaagcaa agttatatat gagtttaagt agagagaatg
     4081 aaactatgct cgagcatcat ttgccagaaa ttaatcagat tgcagattta tttgtgaatg
     4141 agacattggc tttagaagtt cagtgtagcc gactatcaga gcagcgactc agggagcgaa
     4201 caaaagcata tctacaggca gactttcaag tgagatggtt actaggggaa aaactctggt
     4261 taaagcatcg tttaacaaat ctacataaac aatttttaca attttctcaa agtatcggat
     4321 ttcatatttg ggagttagat ttgaggttag aagtactgag actaaaatat ttgatttacg
     4381 aagatttacg cggacatgtc tattacttaa gtaaaacttg ccctcttagt ggagacgttc
     4441 tagcattttt gaagtggcca taccaatcaa aaaatctaaa tttttataag gttaaacaag
     4501 acagaaatat cagagattat gtgcgtcagc agttacgcta tggcaatcaa ttttggttac
     4561 gaaaacaaga aaaggcctac ctttctggtc agaatctatt aacccaagag ctaatgatgt
     4621 tttttcctca aatacagcca ccacgtgtag atactgattt ttgtcaaata acaaattctc
     4681 tgacatcgtt ttatcagaat tttacaaatt attatcaaaa aaataagaat aacttggacc
     4741 aaacacttta tccccctgta ttttatgata aaatagtcac taagaattaa actggaggat
     4801 acaaaatgtc agacaatcgc tcacatattg aagaaaaata ccaatgggat ttaactactg
     4861 tctttgcgac tgatgagtta tgggaaactg aagtggtaga gcttactcaa gccatcgatg
     4921 acgcaaaggg tttttcaggt catctgttgg actcaagcca atctttattg gagattacgg
     4981 aggttgaact agagttatct cgccgtttag aaaaagttta tgtctatgct tcaatgaaaa
     5041 atgaccaaga caccactgtt gcgaaatatc aagaatttca agcgaaagcg acagcgctct
     5101 atgcaaaatt tagtgaaaca ttttcgtttt acgagccaga actattgcaa ctttcagagt
     5161 ctgattacca gtcattcctt ttagaaatgc ctgacttgca aaaatatgat catttctttg
     5221 agaaaatatt tgcaaataaa cctcatgtct tatctcaaaa cgaagaagaa ttgttagctg
     5281 gtgctagtga gatttttgga gcagctggtg agacatttga aatccttgat aatgcagata
     5341 tggtcttccc agttgtaaaa aatgcaaaag gtgaggaagt tgagcttaca catggtaatt
     5401 tcatttccct tatggaatct agcgatagga cggttcgaaa agaagcatat caagctatgt
     5461 atagcacata tgaacaattc cagcatactt acgctaaaac attacaaaca aatgtgaaat
     5521 ctcaaaattt taaggctcgt gtgcatcatt atcaatcagc acgccaatca gctctatctg
     5581 ccaattttat tccagaagag gtctacgaaa ctctaattaa gacagttaat catcatttac
     5641 cgctcttaca tcggtatatg aagttgcgtc aaaaagttct tgggctagat gatttgaaga
     5701 tgtatgacgt atatacaccc ctttctcaaa tggatatgag ttttacttat gatgaagctc
     5761 taaaaaaatc agagaaggtt ctggctattt ttggcgaagc atattctgaa cgtgttcatc
     5821 gtgcttttac tgaacgttgg attgatgtcc atgttaacaa aggaaagagg tcaggcgctt
     5881 attcaggtgg atcttatgac actaatgctt tcatgcttct taactggcaa gatactttag
     5941 acaatcttta tacgctggtg catgaaactg gacatagtct acactcgacg tttactcgtg
     6001 aaaatcaacc ttatgtttat ggtgattatt ctattttcct agctgaaatt gcatcaacaa
     6061 caaatgaaaa tatattaaca gaaacccttt tgaaagaagt taaagatgat aaaaatcgct
     6121 ttgcaattct caatcattat ttggacggtt tcaaaggaac aatttttaga caaacacagt
     6181 tcgctgaatt cgaacatgcc attcatgtag ctgatcagga ggggcaagta ctaactagcg
     6241 aatacttaaa caacctttac gctgagctta atgaaaaata ttatggtttg actaaagaag
     6301 ataatcattt tattcaatat gaatgggcaa gaattccaca cttttactac aattattatg
     6361 ttttccaata tgcaaccggc ttcgcagcag caaattattt agcagagcgt attgtaaacg
     6421 gtaatccgga ggataaagag gcctacctaa attaccttaa agctgggaat tcagattacc
     6481 ctctaaacgt cattgctaaa gccggtgttg atatgactag tgcagactat ctggatgcag
     6541 cttttagagt atttgaagag cgtttagtgg aactggaaaa tcttgtagca aaaggcgtcc
     6601 ataatgatta agttgaaaat atagctattg aagtaaaacc agtcaactag actggttttt
     6661 gatagtgatc agttatttta tagtttgttt tgagagtggt tttggtaaag ttagttagtg
     6721 gaaaactcaa taggtggaaa tacctaccca agctatgatt tttatgagac caaaactgct
     6781 attgtcaaaa aagaagggaa ataggatgaa aaagcttact tttatttggg atttagatgg
     6841 gacattaata gattcgtatg taccaattat ggaagctctt gaagaaacct atcgtcattt
     6901 tggcttaata tttgataaag aattaatcca tgaatatatt ttacaggaat cagtggggca
     6961 attattggta aacctttcag aggaagagca aatacctcat gaaaaactga aagcatattt
     7021 tacaaaagaa caagaaagtc gagattctaa aatacattta atgccatatg caaaagagat
     7081 tttagaatgg accaaagaac aagatattcc caattttatg tatacacata aaggagcaag
     7141 tacgcattca gtgttggaaa ccttgcagat ctctcattat tttgatgaaa ttttaactgg
     7201 tgtttcggga ttcgagcgaa aaccacatcc acaagggatt aattatttag ttaaacgata
     7261 ttctttagat aaatcaatga cttattacat aggagatcgt ccactagatt tggaggttgc
     7321 tcaaaatgc
//
LOCUS       DATRQA010000099         7130 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.099, whole genome shotgun sequence.
ACCESSION   DATRQA010000099 DATRQA010000000
VERSION     DATRQA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.099"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            259..393
                     /gene="rpmH"
                     /locus_tag="VBD27_001519"
     CDS             259..393
                     /gene="rpmH"
                     /locus_tag="VBD27_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO7025522.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            749..1423
                     /locus_tag="VBD27_001520"
     CDS             749..1423
                     /locus_tag="VBD27_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7025523.1"
                     /translation="MRLLVVEDEKSIAEAIQALLADKGYSVDLAFDGDDGLEYILTGL
                     YDLVLLDIMLPKRSGLSVLKRVREAGLETPIIFLTAKSQTYDKVNGLDLGADDYITKP
                     FEADELLARIRLRTRQSSLIRANQLRLGNIRLNTDSHELESKESSVKLSNKEFLLMEV
                     FMRNAKQIIPKNQLISKVWGPSDNSEYNQLEVFISFLRKKLRFLKADIEIITTKGFGY
                     SLEERT"
     gene            1423..2610
                     /locus_tag="VBD27_001521"
     CDS             1423..2610
                     /locus_tag="VBD27_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7025524.1"
                     /translation="MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIV
                     KLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSNNTDLTNDVIEKSSLKLLEQGKKSRK
                     WKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSLYLSRFI
                     VGPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEF
                     LIEDLLNLSRLDEKRSKVNFKKLNLSVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIV
                     GEESQIKQILIILLDNAIRHSLSKSAIQFSLKQARRKAILTISNPSAIYSKEVMDNLF
                     ERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPIDGFWTNT
                     MIN"
     gene            2621..2743
                     /locus_tag="VBD27_001522"
     CDS             2621..2743
                     /locus_tag="VBD27_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEO7025525.1"
                     /translation="MKPYLSFIGRTLLYFGILLLLIYFFAYLGRGQGSFIYNEF"
     gene            2756..4291
                     /gene="dltA"
                     /locus_tag="VBD27_001523"
     CDS             2756..4291
                     /gene="dltA"
                     /locus_tag="VBD27_001523"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEO7025526.1"
                     /translation="MIHDMIKTIEHFAETQADFPVYDILGEVHTYGQLKVDSDSLAAH
                     IDSLGLVEKSPVLVFGGQEYEMLATFVALTKSGHAYIPVDQHSALDRIQAIMTVAQPS
                     LIISIGEFPLEVDNVPILDVSQVSAIFEEKTPYEVTHSVKGDDNYYIIFTSGTTGLPK
                     GVQISHDNLLSFTNWMISDDEFSVPERPQMLAQPPYSFDLSVMYWAPTLAMGGTLFAL
                     PKTVVNDFKKLFATINELPIQVWTSTPSFADMALLSNDFNSETLPQLTHFYFDGEELT
                     VKTAQKLRQRFPKARIVNAYGPTEATVALSAVAITDEMLETCKRLPIGYTKDDSPTYV
                     IDEEGHKLPNGEQGEIIIAGPAVSKGYLNNPEKTAEAFFQFEGLPAYHTGDLGSMTDE
                     GLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIV
                     LKEGVRDDFERDLDLTKAIKEDLKDIMMDYMMPSKFIYREDLPLTPNGKIDIKGLMSE
                     VNK"
     gene            4288..5553
                     /gene="dltB"
                     /locus_tag="VBD27_001524"
     CDS             4288..5553
                     /gene="dltB"
                     /locus_tag="VBD27_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEO7025527.1"
                     /translation="MIQAFLEKLPHLDVYGNPQYFFYLILAVLPIYIGLFFKKRFALY
                     EIIFSLSFIVMMLTGSTFNQLKSLLAYVVGQSLLVFIYKAYRKRFNHTLVFYVTVCLS
                     IFPLFLVKLIPAISEDGHQSLFGFLGISYLTFRAVAMIIEMRDGVLKEFTLWEFLRFL
                     LFFPTFSSGPIDRFKRFNEDYINIPDRNELLDMLGQAIHYLMLGFLYKFILAYIFGSL
                     IMPPLKELALEQGGVFNWPTLGVMYAFGFDLFFDFAGYTMFALAISNLMGIKSPINFD
                     KPFKSRDLKEFWNRWHMSLSFWFRDFVFMRLVKLLVKNKVFKNRNVTSSVAYIINMLL
                     MGFWHGLTWYYIAYGLFHGIGLVINDAWVRKKKNINKERRLAKKPLLPENKWTYALGV
                     FITFNVVMFSFLIFSGFLDLLWFPQPHNK"
     gene            5568..5807
                     /gene="dltC"
                     /locus_tag="VBD27_001525"
     CDS             5568..5807
                     /gene="dltC"
                     /locus_tag="VBD27_001525"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEO7025528.1"
                     /translation="MDIKSEVLAIIDDLFMEDVSSMMDEDLFDAGVLDSMGTVELIVE
                     LESHFNIDIPIAEFGRNDWNTANKIVAGVTELCNA"
     gene            5800..7062
                     /gene="dltD"
                     /locus_tag="VBD27_001526"
     CDS             5800..7062
                     /gene="dltD"
                     /locus_tag="VBD27_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEO7025529.1"
                     /translation="MLKRLGKVFGPLVCALLLLVGLYFVFPVSQPHHLGKEKNSAVAL
                     TKAGFKSRVQKVRAFSDPKANFVPFFGSSEWLRFDAMHPSVLAEAYNRSYIPYLLGQK
                     GAASLTQYYGIQQIKGQIKNKKAIYVISPQWFVRKGANKGAFQNYFSNDQTIRFLQNQ
                     TGTTYDRYAARRLLKLYPEASMSDLIEKVADGQKLSNKDKQRLKFNDWVFEKTDAIFS
                     YLPLGKTYNQAIMPHVGKLPKAFSYNHLSRIASQDAKVATRSNQFGIDDRFYQTRIKK
                     HLKKLKGSQRHFNYTKSPEFNDLQLVLNEFSKQNTDVLFVIPPVNKKWTDYTGLDQKM
                     YQKSVEKIKHQLQSQGFNHIADLSRDGGKPYFMQDTIHLGWNGWLELDKHINPFLTEE
                     NSKPNYHINNKFLKKSWAKYTERPSDYK"
BASE COUNT         2312 a         1103 c         1355 g         2360 t
ORIGIN      
        1 ttatttatca tttttataga gagaacaatt atttcattac ctattattgt tttgatggca
       61 catcttttct tttagttgga gaaaatagag aagcatttct tgctaacaaa tagaactatt
      121 tactgaaaaa agacaaatga acttgaaagt atttcttgac aagaggccta aaattgttat
      181 aaaatagtat ggtatgttta gtaactgatc aaaatagccg gctaaacgaa tacgaaaatc
      241 tatcaggagg tattcaacgt gaaacgtact tatcaaccaa gtaaaatccg tcgccaacgt
      301 aaacacggat tccgtcaccg tatgtcaact aaaaacggac gtcgcgtgct tgctagccgc
      361 cgtcgtaaag gacgtaaagt gttatcagct taatatagat agcaaaataa tatcaagcca
      421 acaggtactc gagtctggtg gcttttttgg taccttcata acgataaagg tttttgtgag
      481 agtagtttga gtatattata gataaagtta atgaaaagtg agtttataat gcggaaattc
      541 gtagacaaat agcccctatt tttgttagaa tatgaggaga tttcttattg agaaatatct
      601 ttatagcgag tgatatttta ttttttagac gcttgtatta ttgaagtagt ttagtgactt
      661 aggaaatact tttattaata tcgttttttc aaggttattt caattttgct agtttatact
      721 ataatatata aggtaaggtg actagaaaat gagacttctt gtagttgagg atgaaaaaag
      781 tatagcagag gctattcagg ctttattggc agataaaggt tattctgttg atttagcatt
      841 tgatggagat gatgggttag aatacatttt gacaggtctc tatgatttag tcctgttgga
      901 tattatgtta cccaaacgct caggattatc tgttttaaaa agagtgaggg aagctggttt
      961 agaaacccca ataatctttt taactgcaaa atcacagact tatgataaag ttaatggttt
     1021 agatttaggg gcagatgatt atatcacaaa accctttgaa gcagatgaat tgctagctag
     1081 aattaggctg agaaccagac aatcttctct aatcagagca aatcaattgc ggcttggaaa
     1141 tataaggctt aacacagatt cacatgagct agaatctaaa gaaagtagtg taaaattatc
     1201 caataaagaa tttctcttga tggaagtttt tatgcgaaat gcaaagcaga ttattcctaa
     1261 aaatcagttg atttctaagg tttggggacc ttcagacaat tcagaatata accagttaga
     1321 agtatttatt tcttttttgc gaaaaaaatt gcgcttttta aaagcagaca ttgaaataat
     1381 aaccactaag ggctttggat attccttgga ggagaggaca taatgtttag tgatttacgc
     1441 aaaaaatttg tctttttgac catgtcaatc cttattgtcg ttgttctctt tctatttgca
     1501 gttagtaatc gctacaatca atattgggat gaatatgatg catataggat tgttaaatta
     1561 gttgccaaaa atgattattt aggaatacca ggcgatgaac cgattgcttt ggtgactata
     1621 gataaccaaa agatggttaa gattcaaagc aataatactg atttgacaaa tgatgttatc
     1681 gaaaaatcta gtttgaagct ccttgagcaa ggaaagaagt cacgaaaatg gaagtctttt
     1741 atatattcca taaaagaata taaagataaa acctacacca tagctattat ggatttggct
     1801 agttatgaag tcccttatgc aaggcgtttt ctaattcttg tttttactat ttttggattt
     1861 tgtttattag cagctgtaag tctttatctt tctcgattta ttgtaggacc tgtagagaca
     1921 gaaatgactc gcgaaaaaca gttcgtttca gatgcgagtc atgagctgaa gacccctatt
     1981 gcagctatac gagccaacgt tcaggttctg gaacagcaaa ttccagggaa tcgttatctg
     2041 gatcatgttg tttcggagac taagcgaatg gagtttttaa ttgaagattt gcttaatttg
     2101 tctagacttg atgaaaaacg ctccaaggta aatttcaaaa agttaaactt atctgtctta
     2161 tgtcaagaag tacttctaac atatgaaagt ttggcatatg aagaagaaaa gtgtttaaat
     2221 gatacgattg aagatgatgt ctggattgtt ggagaagaga gtcagattaa gcaaatttta
     2281 ataattcttt tggataatgc tattaggcat tctttgtcaa aatcagcaat ccagtttagt
     2341 cttaaacaag ctcgaagaaa agctattctg acaatatcaa atccaagtgc tatctattcc
     2401 aaagaggtta tggataatct ctttgagcgt ttttatcaag ctaaagatga tcatgcagat
     2461 tctctcagtt ttggtctagg cttatctatt gctaaagcaa tcgtggaacg ccataagggg
     2521 agaattagag catatcaaga aaaagaccaa ttacgtcttg aagtccagtt accaattgat
     2581 ggattttgga ccaataccat gattaattaa aggaagaatg atgaaaccat atttatcttt
     2641 tattggtaga acgttattat acttcggtat tttattgtta ctaatttact tttttgcata
     2701 ccttggtcgc ggacaaggca gttttattta taatgaattc taggagatgg tacaaatgat
     2761 acatgatatg attaaaacaa ttgagcattt tgctgagaca caagctgatt ttccagtgta
     2821 tgatatttta ggggaagtcc atacttatgg acaacttaaa gtagactctg actctctagc
     2881 tgctcatatt gatagcctag gccttgttga aaaatcacct gtcttagtat tcggtggtca
     2941 agaatatgaa atgttggcga catttgttgc tttaacaaag tcagggcatg cttatatacc
     3001 ggttgaccaa cactctgctt tggatagaat acaggctatt atgacagttg ctcaaccaag
     3061 ccttatcatt tcaattggtg aatttcctct tgaagttgat aatgtcccaa tcctagacgt
     3121 ttctcaagtt tcagctattt ttgaagaaaa gactccttat gaggtaacac attctgttaa
     3181 aggtgatgat aattactata ttattttcac ttcagggact actggtttac caaaaggtgt
     3241 gcaaatttca catgacaatt tattgagctt tacaaattgg atgatttctg atgatgagtt
     3301 ttcagttcct gaaagaccgc aaatgttggc tcaaccgcca tattcatttg acttatcagt
     3361 tatgtattgg gcaccaacac tagctatggg aggcaccctg tttgccctac caaaaacagt
     3421 agttaatgat ttcaaaaaac tattcgctac cattaatgaa ttgccaatac aggtttggac
     3481 ttcgacacca tcatttgctg atatggcgct actatctaac gatttcaatt cagagacctt
     3541 gccacagtta acacattttt attttgatgg ggaagagtta actgtcaaga ctgcacaaaa
     3601 acttcgtcaa cgttttccaa aagctcgtat cgttaatgca tatgggccaa cagaagcaac
     3661 agttgctcta tccgcagtag caattactga tgaaatgtta gaaacatgca aacgccttcc
     3721 aattggttac actaaagatg actcaccaac gtatgtgatt gatgaagaag gtcataaatt
     3781 accaaacgga gagcaaggtg aaatcattat tgctggacca gcagtatcaa aaggctatct
     3841 taacaatcca gaaaagacag ctgaggcatt tttccaattc gaaggtctac ctgcttacca
     3901 taccggtgac ttaggaagta tgaccgatga aggtcttctg ctttacggtg ggcgtatgga
     3961 tttccaaatt aaatttaacg gctatcgtat tgaattagaa gatgtttctc aaaacttaaa
     4021 caaatcgcag tatgtaaaat cagcagtagc agtgccacgt tataacaagg atcataaagt
     4081 tcaaaactta ttagcctata ttgtcttaaa agaaggtgta agagatgatt ttgaacgtga
     4141 tttggatttg acaaaagcaa ttaaggaaga cttaaaggac attatgatgg attacatgat
     4201 gccatctaaa tttatctatc gagaggattt acctttgaca ccaaatggga aaattgatat
     4261 caaaggtctt atgagcgagg taaacaagtg atacaggcat ttttagaaaa acttcctcac
     4321 ttggatgttt atgggaatcc acaatatttc ttctacctta tcttagctgt cctaccaatt
     4381 tacatcggct tattctttaa gaagcgtttt gccttatatg agattatttt tagtctaagt
     4441 tttattgtaa tgatgttgac tggtagtact tttaatcaat tgaagtcact attggcatac
     4501 gttgtcggac agtctctgct agtttttatc tataaagctt accggaaacg atttaatcat
     4561 actttggtct tttatgtaac ggtttgttta tctatttttc cgctattttt ggtaaaatta
     4621 attccagcta tatctgagga tgggcatcag tcactttttg ggtttctagg aatttcttac
     4681 cttactttta gagctgttgc tatgattatt gaaatgagag acggtgtctt gaaagaattt
     4741 actttatggg aattcttaag atttttactc ttctttccaa ctttctcaag tggaccaatt
     4801 gatcgtttta aacgattcaa tgaggattac attaatatcc cagatcgaaa cgaactccta
     4861 gatatgttag gtcaagcgat tcattatttg atgttaggtt ttctctataa gtttatttta
     4921 gcctatattt ttggaagtct gattatgcct cctctaaaag aattagcgct agaacagggt
     4981 ggtgtgttta attggccaac acttggggtt atgtatgcct ttggttttga tttgttcttt
     5041 gattttgcag gttacacaat gtttgcgttg gctatttcta acctaatggg gattaagtct
     5101 ccgattaact ttgacaaacc tttcaaatca cgcgacctaa aagaattttg gaatagatgg
     5161 catatgagcc tttctttctg gtttagagac tttgttttca tgaggcttgt taagctttta
     5221 gttaaaaata aagtttttaa aaaccgtaat gttacttcaa gtgtagctta tattatcaat
     5281 atgcttctta tgggattctg gcatgggtta acttggtact atatagccta tggtctcttt
     5341 catgggattg gcctagttat taatgacgct tgggtacgta agaagaaaaa tattaataaa
     5401 gaaagaagat tggctaaaaa accactttta ccagaaaaca aatggactta tgctttgggt
     5461 gtcttcatca cctttaatgt agttatgttt tctttcttga ttttttcagg atttttagat
     5521 cttttgtggt tcccacaacc gcataacaaa taataaggag tattataatg gatattaaat
     5581 cagaagtatt agcaattatt gatgacctat ttatggagga tgtttcttct atgatggatg
     5641 aagacttatt tgacgcagga gttctagaca gcatgggtac tgttgaacta attgttgagt
     5701 tggaaagcca ctttaatatt gatattccga ttgcggaatt tggtcgtaat gactggaaca
     5761 cagctaataa aattgttgct ggagtaacgg agctctgcaa tgcttaaacg tctaggtaaa
     5821 gtatttggac ctctagtttg tgctttacta ttgttggtag gattatattt tgtttttcct
     5881 gtttctcagc ctcatcattt aggtaaggaa aaaaacagtg cagtagcgtt gacgaaggca
     5941 ggttttaaaa gcagagttca aaaagttaga gctttcagtg atcctaaagc caattttgtc
     6001 cctttctttg gttcaagtga gtggctaaga tttgacgcaa tgcatccatc agttttagca
     6061 gaggcttaca ataggtctta tatcccatat cttttaggtc aaaaaggggc ggcttctctg
     6121 acacaatact atggcattca acagattaaa ggacaaatca aaaataaaaa agctatctat
     6181 gttatttctc cgcaatggtt tgttcgcaag ggagccaaca aaggtgcttt tcaaaactat
     6241 ttcagcaacg atcaaaccat tcgatttttg caaaatcaaa cagggacaac ctacgatagg
     6301 tatgctgctc gtcgattgtt aaaattatat cctgaagctt ctatgtcaga tttgatagaa
     6361 aaagttgcag atggccaaaa actatcaaat aaagacaaac aaagactaaa gtttaatgat
     6421 tgggtatttg agaagacaga tgctattttt agctatctac cactaggaaa aacttataat
     6481 caggcaataa tgcctcatgt tggtaaatta ccgaaagcat tctcatataa tcatttatcg
     6541 cgtattgcat cacaagatgc taaagtagca acgagatcaa atcaatttgg tattgatgat
     6601 cgcttttacc aaacaagaat taaaaagcac ttaaaaaaat tgaaagggtc acaacgacat
     6661 ttcaattata ctaagtcacc agaatttaat gatttacagt tggttcttaa tgaattctca
     6721 aaacaaaata cagatgtcct ttttgtcata ccaccagtaa ataaaaagtg gacagactac
     6781 acagggcttg atcaaaaaat gtatcaaaaa tctgtagaaa aaataaaaca ccaacttcaa
     6841 agtcaaggtt tcaatcatat cgctgacctt tctcgagatg gaggtaagcc atactttatg
     6901 caagatacaa tccatttagg ttggaatggt tggttagagc tagataagca tatcaatcca
     6961 tttttaacag aggaaaacag caagccgaat tatcacatta ataataaatt tttgaagaaa
     7021 tcttgggcaa aatatacaga acgtccaagt gattacaagt aaatagtaga gtcagatgat
     7081 ttatagcatt agagctatta atcatctttt ttaatatctt tgtatttggt
//
LOCUS       DATRQA010000100         7072 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.100, whole genome shotgun sequence.
ACCESSION   DATRQA010000100 DATRQA010000000
VERSION     DATRQA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.100"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..843
                     /locus_tag="VBD27_001527"
     CDS             <1..843
                     /locus_tag="VBD27_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO7025530.1"
                     /translation="KNASTIVGKIQELYHTEDISVFYTEQKDDEKKQVINILRSFKES
                     DHLMIIGGDGTLSKVMTYLPQHIPCAYYPVGSGNDFARALKIPNLKETLTAIQTERLK
                     EINCFIYDKGLILNSLDLGFAAYVVWKASNSKIKNILNRYRLGKITYIVIAIKSLLHS
                     SKVQVLVEGETGQRIKLNDLYFFALANNTYFGGGITIWPKASALTAELDMVYAKGHTF
                     LKRLSILLSLVFKRHTTSKSIKHQTFKAMTVYFPKNSLIEIDGEIVELDQISLKCQKR
                     YLYM"
     gene            853..1050
                     /locus_tag="VBD27_001528"
     CDS             853..1050
                     /locus_tag="VBD27_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO7025531.1"
                     /translation="MYQVGSLIEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            1253..1369
                     /locus_tag="VBD27_001529"
     CDS             1253..1369
                     /locus_tag="VBD27_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025532.1"
                     /translation="MDNLIRLAEIFDVSLDEHVLAKTNEVKVERIYEKNLDL"
     gene            1613..2728
                     /gene="ychF"
                     /locus_tag="VBD27_001530"
     CDS             1613..2728
                     /gene="ychF"
                     /locus_tag="VBD27_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO7025533.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ADPDDIDYVNQIRAFAETESAEVVVISARAEEEISELDDEDKLEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2812..3387
                     /locus_tag="VBD27_001531"
     CDS             2812..3387
                     /locus_tag="VBD27_001531"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO7025534.1"
                     /translation="MVKMIVGLGNPGSKYNDTKHNIGFMAVDRIVKKLDVNFTEDKNF
                     KAEIGSDFINGEKIYFIKPTTFMNNSGIAVKALLTYYNISIKDMIIIYDDLDMEVGKI
                     RFRQKGSAGGHNGIKSIIAHLGTQEFDRIKVGIGRPNGRMTVINHVLGKFDKNDEIMI
                     SNTLDKVDNAVNYYLQTNDFQKTMQKYNGLK"
     gene            3384..6881
                     /gene="mfd"
                     /locus_tag="VBD27_001532"
     CDS             3384..6881
                     /gene="mfd"
                     /locus_tag="VBD27_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO7025535.1"
                     /translation="MNIIELFSQNKVVRTWHSGLVTNSRQLVMGFSGASKAIAIASAY
                     EKLSKKIMVVTATQNDSDKLSSDISSLIGEDNVYQFFADDVPAAEFIFSSLDKSISRL
                     SALRFLKDPEKNGVLITSISGLRLLLPNPEVFSKSQYKFEIGQECYLDKLSKNLVNLG
                     YQKVSQVFSPGEFSQRGDILDIFEMTQEYPYRLEFFGDEIDGIRQFDIDTQKSLKQLE
                     SVQISPADDIILQDADFERAKKKLEGYLVTASEEQRAYLSEVLSTTENHFKHSNIRRF
                     LSIFYEKEWGILDYIPEGTPLFVDDFQKIVDRNAKLDLEIASLLTEDLQQGKSHSSLN
                     YFSDPYKQLRQYQPATFFSNFHKGLGNLKFDKLHHFTQYGMQEFFNQFPLLVDEINRY
                     KKSGATVLLQVDSQKGLNLLQENLKEYGLDLIISDKNDIVPKESQLIVGHLSNGFYFA
                     DEKIVLITEREIYHRRVKRKIRRSNISNAERLKDYNELSVGDYVVHNVHGVGKFLGIE
                     TIEIQGIHRDYLTIQYQNADRISIPVEQIELLTKYVSADGKEPKINTLNDGRFKKAKQ
                     RVAKQVEDIADDLLKLYAERSQLQGFAFSPDDNMQNDFDNDFAYVETEDQLRSIKEIK
                     QDMEGNRPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHFENFKE
                     RFSNYPVTVDVLSRFRSKKEQTDTLKRLSKGQVDIIIGTHRLLSQDVVFSDLGLIVID
                     EEQRFGVKHKEKLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLETNPGLVREAIIREIDRGGQVFYVYNKVDTIEQKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFVENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEISEKRLDAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIATKQGKSLIRQKGNVELALQIDAYLPAEYISDERQK
                     IEIYKRIRELETRADYEALQDELIDRFGEYPDQVAYLLEIGLLKAYLDLAFTELVERK
                     GNEISIIFEKASLKYFLTQDYFEALSKTQLKARISETNGKMEVVFNIKHKKNYEIIEE
                     LLKFAECFIEIKSRKPVEE"
BASE COUNT         2477 a          964 c         1364 g         2267 t
ORIGIN      
        1 aaaaatgcct ccactattgt tggtaaaatt caggagcttt atcatactga agatatttct
       61 gtgttctata cagaacagaa agatgatgaa aaaaaacaag tcattaatat actaaggtct
      121 tttaaagaaa gtgatcatct aatgattata ggaggagatg gtaccttatc aaaagtaatg
      181 acttatctcc cccaacatat tccgtgcgct tattatcctg ttggttcagg aaatgatttt
      241 gccagggctt tgaaaattcc taacctcaaa gaaaccctta ccgctattca gacagaaaga
      301 ttaaaggaaa ttaattgctt tatttatgat aaagggctga ttttgaatag tttagattta
      361 ggttttgcag cctatgtcgt ttggaaagca tctaactcta aaataaaaaa tattttgaat
      421 agatatcgtc taggaaaaat aacttacatt gttatagcta tcaaatcatt attacattct
      481 tctaaagtac aagtattagt ggaaggagag acaggacagc ggataaaact aaatgacctt
      541 tattttttcg cattagcaaa taacacttat tttggaggtg gcattactat ttggcctaaa
      601 gcaagtgctt taactgcaga gttagatatg gtttacgcta aagggcatac ttttctaaaa
      661 cgattaagta ttttattatc tttggttttt aaaaggcata ctacctctaa aagtataaaa
      721 caccagactt tcaaagctat gaccgtatat tttcctaaaa atagtttgat tgaaatagat
      781 ggcgaaatag ttgagttaga tcagattagt ttaaaatgtc aaaaaaggta tttatatatg
      841 taaggagaaa agatgtatca agttggatca cttattgaaa tgaaaaaacc tcatgcttgt
      901 gtaattaaag aaactggtaa aaaggctaat caatggaagg tactgagagt aggagctgat
      961 attaaaattc agtgtactaa ctgtcagcat gtgattatga tgagtcgtta tgattttgag
     1021 cgaaaattaa aaaaagtact gcaaccatga aagtcctttt aaacaaataa ttatagctat
     1081 atttgcaacc aatactttcc taaataattg ctagtatgcc gttggaaaat tagctattct
     1141 aatgttatcg aaagaagaaa ggtggccatt gacaatgacg aaagttgcag aacagttgtt
     1201 tatttttcgg taagtcatat caaaatggga aaatggggat accacaccag atttggataa
     1261 tttaatcagg ttagctgaga tttttgacgt gagcttagat gaacatgttt tagctaaaac
     1321 aaatgaagtt aaagttgaac gtatttatga aaaaaatctt gatctgtaaa aatataataa
     1381 aatttattgg tttatttttc gaaatattat tcgatctcta ctaattatct tagctatatt
     1441 aactttctta taagctctag gtcttccctt tgtttcggag tggataattt aaatgaaggt
     1501 gaaagagtgg cataatgata agcaaatctg aaattttctt cttatcatac cgctttttct
     1561 tctatttttt gatataatgg tcttgattga aatttgaatg gagactcact aaatggcttt
     1621 aacagcaggt attgtaggct taccaaatgt tggtaaatca actttattta acgcaattac
     1681 aaaagcagga gcagaagctg ctaactatcc ttttgcgact attgatccca atgttggtat
     1741 ggtagaggta ccagatgagc gtctgcaaaa attgacagaa ttgattacgc ctaaaaaaac
     1801 agttccgaca acctttgaat ttactgatat tgcaggtatt gttaaaggtg cttcgaaggg
     1861 ggaaggtcta ggaaataaat ttttagctaa tattcgtgaa gtggatgcta ttgttcatgt
     1921 ggtacgtgct tttgatgatg aaaatgttat gcgtgaacaa ggtcgtgagg atgccttcgt
     1981 tgatcctata gcagatattg atactattaa tcttgaatta atcttagctg atttagagtc
     2041 aatcaataaa cgttatgcgc gtgttgaaaa aatggcacga actcaaaaag ataaagaatc
     2101 agtagcagag ttcaatgttt tacaaaagat taaacctgtt ttggaagatg ggaaatcagc
     2161 tcgtaccatt gagtttacag aagaggaagc aaaagttgtt aaaggtctct ttttattaac
     2221 aactaaacct gttttatatg ttgctaatgt tgatgaggac aaagtagctg atcctgatga
     2281 tatcgactat gttaatcaaa ttcgtgcatt tgcagaaaca gagagtgcag aagtagttgt
     2341 gatttctgct cgagctgaag aagaaatatc tgaacttgat gatgaagata agttagaatt
     2401 tttagaagct attggattaa cagaatctgg tgttgataaa ttaacacgag ctgcttatca
     2461 tcttttaggc cttggaactt actttactgc tggtgaaaaa gaggttcgcg cttggacatt
     2521 taagcgtggt ataaaagctc cacaagctgc gggtattatc cattcagatt ttgaaagagg
     2581 ttttattcgt gcagtaacga tgtcatatga tgaccttatt caatatggat ctgaaaaagc
     2641 agtaaaagaa gctggccggt tacgtgaaga aggaaaagag tatattgttc aagatggcga
     2701 tatcatggaa ttccgtttca atgtctagta ataaataatc aatttgttaa cttttaggtt
     2761 aaggatattg ttattatcct taaccatttt tgcgttttaa tggagaagat aatggtaaaa
     2821 atgattgttg gattaggaaa tccaggaagt aaatataatg atacaaaaca taatattgga
     2881 tttatggctg ttgacagaat tgtaaaaaaa cttgacgtca actttacaga agataaaaat
     2941 tttaaagcag agataggtag tgattttatc aacggtgaaa aaatttattt tatcaaacca
     3001 actactttta tgaataacag tggtattgcg gtaaaagccc tgcttactta ctacaacatt
     3061 tctattaagg atatgattat catatatgat gatttagaca tggaagtcgg taaaatacgt
     3121 tttcgtcaaa aaggatctgc tgggggacat aatggtatca agtctattat tgctcattta
     3181 ggtactcaag aatttgatcg tattaaagta ggtataggac gtccgaatgg acgtatgaca
     3241 gttattaatc atgttctagg gaagtttgat aaaaatgatg aaattatgat atcaaatact
     3301 cttgataagg ttgacaatgc tgtcaattat tatttacaga cgaatgattt tcaaaaaaca
     3361 atgcagaaat acaacggttt aaaatgaata ttatagaatt atttagccaa aataaagtgg
     3421 tgcggacatg gcactctgga ttagtaacta atagtcgcca attagttatg gggttttcag
     3481 gagcaagtaa agctattgct atagcatcag cctacgagaa gttatctaaa aaaattatgg
     3541 tagtcactgc cactcaaaac gattctgata aattatcaag tgatatatca agtttaattg
     3601 gagaagataa cgtttatcaa ttttttgctg atgacgttcc tgcagcagag tttattttct
     3661 cttcattaga caaatcaatt tcacgactca gtgccttgag gtttctaaaa gatccagaaa
     3721 aaaacggtgt tttgattact agtatcagtg gtttaaggtt actattacca aatccggaag
     3781 ttttctcgaa aagtcaatat aagtttgaaa tcggtcaaga atgttattta gataaattga
     3841 gtaagaactt agtcaattta ggttatcaga aagtttctca agtatttagt cctggtgaat
     3901 ttagccaaag aggagatatt ttagatattt ttgagatgac acaagaatat ccttatcgct
     3961 tggaattctt cggagatgaa atagatggca tcaggcagtt tgatattgac actcaaaagt
     4021 ctcttaaaca attagagtca gtacagattt cgccagcaga tgatattatt ttacaagatg
     4081 cagatttcga gcgtgcaaag aaaaaacttg agggctatct agtaacagct agtgaggaac
     4141 aacgcgccta tttgagcgag gttttatcta caaccgagaa tcattttaag cactcaaata
     4201 ttcgtcgttt tctaagtatc ttttatgaaa aagaatgggg aatattagac tatattcctg
     4261 agggaactcc actttttgtt gatgattttc aaaaaattgt agatcgaaat gctaagttag
     4321 atttagaaat agctagcctt ctgacggaag atttacaaca aggcaagtct cattctagtt
     4381 tgaactattt ttcagatcct tataagcaat taagacaata ccaaccagca accttctttt
     4441 caaatttcca caaaggatta ggaaatctga aatttgataa acttcatcat tttactcaat
     4501 atggaatgca agaatttttt aatcaatttc cattgttggt cgatgaaatc aatcgttata
     4561 agaaatctgg agcgacagta ctgttgcaag tagattctca aaaaggttta aatctccttc
     4621 aagaaaactt aaaagaatat gggttagatt taatcatttc agataaaaat gatatcgttc
     4681 caaaggagtc acaacttatt gttgggcatc tttcaaatgg tttttatttt gcagatgaga
     4741 aaattgttct gattactgaa cgtgagattt atcataggcg tgttaaaagg aaaattagac
     4801 gcagtaatat tagtaatgca gaacgtctaa aagattataa tgaactttca gttggcgatt
     4861 atgttgttca taatgttcat ggcgtaggaa aatttttagg tattgaaaca attgagatac
     4921 aaggtattca tcgtgattat ctgactattc aatatcaaaa tgctgatcgg atctctatcc
     4981 ccgtagaaca aattgaactt ctaacaaaat atgtctctgc agatggcaaa gagcctaaaa
     5041 ttaatacgtt aaatgatggt cgcttcaaaa aagcaaaaca gagagttgca aagcaagtag
     5101 aagatattgc tgatgatttg ttgaaacttt atgcagagcg tagtcaatta caaggatttg
     5161 cattttctcc tgacgataat atgcaaaatg actttgataa tgattttgca tatgtagaga
     5221 cagaggatca attgcggtcg ataaaagaaa ttaaacagga tatggaaggt aatcgtccga
     5281 tggatcgatt attggttgga gacgttggtt ttggtaagac agaggttgct atgcgtgcgg
     5341 ctttcaaagc tgtcaatgac cataaacaag ttgctgtttt agtcccaaca acagttctag
     5401 ctcagcagca ttttgaaaat tttaaagaac gttttagtaa ttatcctgtt acagttgatg
     5461 tacttagtcg ttttcgtagc aaaaaagaac aaacagatac actaaaaaga ttatctaagg
     5521 gccaagttga tattattatt ggaacacatc ggcttctctc tcaagatgtt gtattttcgg
     5581 atttagggtt aatagttatt gatgaggaac agcgatttgg tgtaaagcat aaagaaaagt
     5641 taaaagaatt aaaaacaaaa gttgatgtct taactttgac agcgacccca atacctagaa
     5701 cattacatat gtcaatgctt ggcattagag acttatcagt cattgaaaca cctccaacta
     5761 atcgttatcc tgtgcagact tacgttttgg agacaaatcc tggtttagtt agagaagcta
     5821 ttattcgtga gatagacaga ggaggacagg ttttttatgt ttacaataaa gttgacacta
     5881 ttgaacagaa agtttcagaa cttcaggagt tagtaccaga agctagtatt ggttttgttc
     5941 atggtcaaat gtctgaaatt caattagaaa atacattaat agactttatc aatggagatt
     6001 acgatgtttt agttgcaacg acaataattg agacaggagt cgatatttca aatgttaata
     6061 ccctctttgt tgaaaatgca gatcatatgg gcttgtcaac cctttatcaa ttaagaggcc
     6121 gtgtgggtag aagtaatcgc attgcttatg cttaccttat gtacagacct gataaagttt
     6181 taacagaaat ttctgagaaa agactagatg caatcaaagg gtttacagaa ttgggatctg
     6241 gttttaaaat agcgatgcgt gatttatcta tacgtggtgc aggaaatatt ttaggagctt
     6301 cacaaagtgg ttttattgat tcggttggat tcgagatgta ttcacagctt ctagagcaag
     6361 cgattgccac caaacaagga aaatcattaa ttcgtcaaaa aggtaatgtt gaattagcct
     6421 tacaaattga cgcctattta ccagcagaat atatatcaga tgagcgccaa aaaatagaaa
     6481 tatataagcg tattcgtgaa ttagaaacta gggctgatta tgaagcctta caagatgagt
     6541 taatagatcg ttttggagaa tatcctgatc aagttgcata ccttttagaa ataggacttt
     6601 taaaagctta tctggatctt gcctttactg aattagtaga aagaaaaggt aatgaaataa
     6661 gcatcatatt tgaaaaagca tctttgaaat attttttaac tcaagattat tttgaagcac
     6721 tatcaaaaac tcaacttaag gctagaatta gcgaaacaaa tggtaagatg gaagttgttt
     6781 ttaatatcaa acataagaaa aattatgaaa ttattgaaga acttctaaaa tttgccgaat
     6841 gttttatcga gattaagtca cgtaaaccag tagaagaata actaagtgaa aagttgcaag
     6901 gaaatttgga tactctttta gaaaatgttt ggttcaagaa gaatagtacg agaaacatta
     6961 tgtttcacgt caaacatctc aacttttgaa agacataaat atttttagag gttattaaaa
     7021 tagaagtttt ttacttgata tttgagagaa ttaagagagc taatagctct ct
//
LOCUS       DATRQA010000101         7070 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.101, whole genome shotgun sequence.
ACCESSION   DATRQA010000101 DATRQA010000000
VERSION     DATRQA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.101"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..328
                     /locus_tag="VBD27_001533"
     CDS             <1..328
                     /locus_tag="VBD27_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7025536.1"
                     /translation="LNAVEAIDKQKKGTISVHTEELEDFIKIEISDNGPGIPDKMKHM
                     IFKPGFSTKFDANGDIYRGIGLSHVRILMEEQYQGTITVCPNQPNGTTFTLLFRKNRF
                     KLGDNT"
     gene            325..1158
                     /locus_tag="VBD27_001534"
     CDS             325..1158
                     /locus_tag="VBD27_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEO7025537.1"
                     /translation="MKFYIIDDDPTITMILQDIIEEDFNNTVVRVNNVSSKAYNELLI
                     ADVDIVLIDLLMPILDGVTLVQKIYKQRSDLKFIMISQVKDNDLRQEAYKAGIEFFIN
                     KPINIIEVKSVVKRVTDTIEMQKKLNTIQNLLENTPSYQKPITTSNLTKIRSILSYLG
                     ITSETAYTDILNICELLLKQELNFAQFDFQKELSIDEHQQKIILQRIRRAVKKAMINM
                     AHLYIDDFENELTLQYANALFGFQNIHNEAQLIQGKSMYGGKISLKRFFDELILQSKT
                     F"
     gene            1380..2102
                     /locus_tag="VBD27_001535"
     CDS             1380..2102
                     /locus_tag="VBD27_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025538.1"
                     /translation="MISFENVSKSYGDHTIIDNISCHIQRGEFFVLVGASGSGKTTIL
                     KMINRLIEPSQGAITLDGENITSLDLRQLRLETGYVLQQIALFPNLTVGENIELIPEM
                     KGWSKGDQKKAASDLLDKVGLPAKDYFNRYPHELSGGEQQRIGILRAIVAKPKVLLMD
                     EPFSALDPISRRQLQDITKQLQSELGITLVFVTHDMKEAMRLADRICVIKEGKIVQLD
                     RPEIIQNNPSDQFVRTLFEEEN"
     gene            2105..3619
                     /locus_tag="VBD27_001536"
     CDS             2105..3619
                     /locus_tag="VBD27_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEO7025539.1"
                     /translation="MTTLITTFQERFGDWTQSLIEHLQLSLLTLILATLIAIPLGIII
                     SHYKKISHVVLQITGIFQTIPSLALLGLFIPFMGIGTVPAVVALIIYALFPILQNTVT
                     ALMQIDANLIEAATAFGMTRWERLKKFELALSMPVIISGIRTASVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNPSLILIGAISSAVLAIIFSGLIGLLEKARLRTIAVSGILLLA
                     GLGLSYAPKWMPGTNTATITVAGKLGTEPDILINMYKELIEDQTDIKVKLKPNFGKTT
                     FLYQALKSGDIDLYPEFTGTITSSLLKNPPKVSNNPKQVYNLAKNGILKQDKLSLLSP
                     MAYQNTYAVAVKKDYAEANQLKNISDLKKLDKLKAGFTLEFKDREDGSIGLQKHYGLN
                     LDISTLEPALRYQAINSKDVNIIDAYSTDSELIQYQLQILKDDKHLFPPYQGAPLLRQ
                     DTIKKYPQVKKALNKLAGHITEKEMQEMNYQVAVKHKSAATVAKQYLKAHHIIK"
     gene            complement(3698..6238)
                     /gene="mprF"
                     /locus_tag="VBD27_001537"
     CDS             complement(3698..6238)
                     /gene="mprF"
                     /locus_tag="VBD27_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEO7025540.1"
                     /translation="MKKLIEKVKSLTSVIKIVFFISVLVLIIVEMIHLKRTISVEQLK
                     SVFGQLSPMNLFLIILVGVIAVLPTTGYDFVLNGLLRTDKSKRYILQTSWCINTFNNL
                     SGFGGLIDIGLRMAFYGKKGQEKSDLREVTRFLPYLISGLSFISVIALIMSHIFHAKA
                     SVDYYYLVLIGASMYFPVIYWISGHKGSHYFGDMPSSTRIKLGIVSFFEWGCAAAAFI
                     IIGYLMGIHLPVYKILPLFCIGCAVGIVSLIPGGLGSFELVLFTGFAAEGLPKETVVA
                     WLLLYRLAYYIIPFFAGIYFFIHYLGSQINQRYENVPKELVSTVLQTMVSHLMRILGA
                     FLIFSTAFFENITYIMWLQKLGLDPLQEQMLWQFPGLLLGVCFILLARTIDQKVKNAF
                     PIAIIWITLTLFYLNLGHISWRLSFWFILLLLGLLVIKPTLYKKQFIYSWEERIKDGI
                     IIVSLMGVLFYIAGLLFPIRAHITGGSIERLHYIIAWEPIALATLILTLVYLCLVKIL
                     QGKSCQIGDVFNVDRYKKLLQAYGGSSDSGLAFLNDKRLYWYQKNGEDCVAFQFVIVN
                     NKCLIMGEPAGDDTYIREAIESFIDDADKLDYDLVFYSIGQKLTLLLHEYGFDFMKVG
                     EDALVNLETFTLKGNKYKPFRNALNRVEKDGFYFEVVQSPHSQELLNSLEEISNTWLE
                     GRPEKGFSLGYFNKDYFQQAPIALVKNAEHEVVAFANIMPNYEKSIISIDLMRHDKQK
                     IPNGVMDFLFLSLFSYYQEKGYHYFDLGMAPLSGVGRVETSFAKERMAYLVYHFGSHF
                     YSFNGLHKYKKKFTPLWSERYISCSRSSWLICAICALLMEDSKIKIVK"
     gene            complement(6222..6965)
                     /locus_tag="VBD27_001538"
     CDS             complement(6222..6965)
                     /locus_tag="VBD27_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7025541.1"
                     /translation="MTENWLHTKDGSDIYYRVVGQGQPIVFLHGNSLSSRYFDKQIAY
                     FSKYYQVIVMDSRGHGKSHAKLNTISFRQIAVDLKDILVHLEIDKVILVGHSDGANLA
                     LVFQTMFPDMVRGLLLNSGNLTIHGQRWWDILLVRIAYKFLHYLGKLFPYMRQKAQVI
                     SLMLEDLKISPADLQHVSTPVMVLVGNKDIIKLNHSKKLASYFPRGEFYSLVGFGHHI
                     IKQDSHVFNIIAKKFINDTLKGEIVEKAN"
BASE COUNT         2541 a         1325 c         1085 g         2119 t
ORIGIN      
        1 tttaaatgca gtagaagcta ttgataaaca aaaaaagggt acaatcagtg ttcacacaga
       61 agaattagaa gattttatta aaattgagat tagtgataac ggaccaggaa tacctgataa
      121 aatgaaacac atgattttca aacctggatt ttcgactaaa tttgatgcta atggtgatat
      181 ctatcgagga atcgggctat cccacgtgcg tattttaatg gaagagcaat atcaaggaac
      241 gataactgtt tgtcctaatc agccaaatgg aacaacattt acactgttat tccgtaaaaa
      301 tcgatttaag ttaggagata atacgtgaaa ttctatatta ttgatgatga ccccactatc
      361 actatgattt tacaagatat tattgaagaa gatttcaata atacagtagt cagagtgaat
      421 aatgtctcca gtaaagcata caacgaattg ctgattgcag atgttgatat cgtattgatt
      481 gatttattaa tgccaatatt agacggtgta acattagtcc aaaaaatcta taaacagcgt
      541 tccgatttaa aatttattat gatttcccaa gtcaaagata atgatttacg acaagaagca
      601 tataaagcag gaatcgaatt ttttattaat aaacccatta atattattga agtaaaaagc
      661 gtcgtgaaac gtgtcactga tactatcgaa atgcaaaaaa aattaaatac aatccaaaat
      721 ctcctagaaa atacgccatc atatcaaaag cctataacga ctagtaacct cactaaaatc
      781 cgttcaatac tcagttattt aggtattact tcagagacag cctacacaga tattcttaac
      841 atctgtgaac ttttattaaa acaagaatta aattttgccc aatttgattt tcaaaaagaa
      901 ctttctatcg atgaacacca acaaaaaatc attttacaac gtattcgtcg tgccgttaaa
      961 aaagctatga taaatatggc tcatctgtac attgatgatt ttgaaaatga attaacactg
     1021 caatatgcta atgcattgtt tggatttcag aatatccata atgaggcaca acttattcaa
     1081 ggaaaaagca tgtatggtgg taaaatttca cttaaacgtt ttttcgatga acttatttta
     1141 cagagtaaaa cgttttaaga cttgtttaag catggcctta tatactataa ccatcctaaa
     1201 acttttcttt tcataaatct ccaacaaaca ccttgtctac cgcaaggtgt ttgtttttgc
     1261 ttctagttta tcgtttctga caatttaaat gtcagatagt tgtttgaatg tctactttat
     1321 gctaatataa ccctaatatc atacgaccta cactcgttac gcactagaaa gtaaattaca
     1381 tgatttcatt tgaaaatgtt agtaaatcat atggtgatca taccatcatt gataatatta
     1441 gttgccatat tcaaagagga gaattctttg tccttgtggg ggccagtggc agtggtaaaa
     1501 ctaccatcct caagatgatt aatcgtctca tcgaaccaag ccaaggcgct attactttag
     1561 atggggaaaa cattacatca cttgatttgc gacaattacg cttagaaact ggttatgtct
     1621 tgcaacaaat tgcacttttc cctaatttaa cagttggaga aaatatagaa cttatccctg
     1681 aaatgaaggg ctggtcaaaa ggtgaccaaa aaaaagccgc tagcgacctt cttgataagg
     1741 tgggtttacc tgctaaggac tactttaatc gctatcctca tgagctctct ggtggggaac
     1801 aacaacgcat aggtatccta cgagcaatcg ttgctaaacc taaggtacta cttatggatg
     1861 agccattttc tgctttagac cctatctcaa gacgtcaatt acaagatatc accaaacaat
     1921 tacagtctga attgggtatt acattagtgt ttgtcaccca tgacatgaag gaagctatgc
     1981 gtcttgcaga tagaatttgt gttattaagg aaggtaagat agtgcaattg gatagaccag
     2041 aaataattca aaataatccc tctgaccaat ttgttagaac cctatttgaa gaggagaact
     2101 gagtatgaca acactaatca ctacctttca agagcgtttt ggagattgga ctcaaagtct
     2161 tattgaacac ctccagctat cactactaac acttatttta gcaactttaa ttgctattcc
     2221 actcggtatc atcattagtc attataaaaa aatcagtcat gttgtccttc agataactgg
     2281 gatatttcaa acaattcctt ctttagcctt gcttggttta tttatacctt ttatgggaat
     2341 tggaactgta ccagctgtcg ttgcactaat tatttacgct ctatttccaa ttttacaaaa
     2401 tactgtaaca gcactgatgc aaattgatgc taatctcatt gaggcagcaa ccgcttttgg
     2461 aatgacacgt tgggaaaggc ttaaaaagtt tgagttagct ttaagtatgc ctgttatcat
     2521 ctcaggcatt agaactgcct ctgttatgat tattggtaca gctacattag ctagtcttat
     2581 cggagctggt ggccttggat catttatttt gttagggatt gatcgcaaca atccttctct
     2641 aattctcata ggggcaatct cttctgctgt cttagctatt atatttagtg gtttaattgg
     2701 attactagaa aaggctagat tacgaaccat agccgtttct ggtatacttt tactagcagg
     2761 cttaggacta tcctatgctc caaaatggat gcctggaaca aatactgcaa cgataactgt
     2821 tgcagggaag ttaggaactg aaccagatat tttaattaat atgtacaaag aacttattga
     2881 ggatcagaca gatatcaaag ttaagcttaa acctaatttc ggaaaaacaa ctttcttata
     2941 tcaagcccta aaatctggtg atattgatct ttacccagag tttacaggta ctattacatc
     3001 aagtttactt aaaaatcctc ctaaagtaag taataaccct aaacaggttt acaacttagc
     3061 caaaaacggt attttaaaac aagataagtt gtcacttttg tcacctatgg cttatcaaaa
     3121 cacctacgct gtagctgtta aaaaagatta cgctgaagct aatcaactca aaaatatcag
     3181 cgacctaaaa aaattagata aactaaaagc tggtttcact ttagaattta aggatagaga
     3241 ggatgggagt attggtttac agaaacatta tggtttaaac cttgatatct caaccttaga
     3301 acctgcttta cgataccaag ctatcaatag taaagatgtc aatattattg acgcttactc
     3361 taccgatagt gaattaatcc agtatcaact tcaaattctc aaagacgata agcatctatt
     3421 tccgccatat caaggagcac cgctactaag gcaagatacg attaaaaaat accctcaagt
     3481 aaaaaaagct ctcaataaac ttgctggtca tatcactgaa aaagaaatgc aagaaatgaa
     3541 ctaccaagta gctgtaaagc ataaatcagc agctacagta gctaaacaat atttaaaagc
     3601 acatcatatt ataaagtaga ttcaagcaat caatggtatt aaatgattta aaaagagcat
     3661 gtcgcatgac atgctctttt taattgccaa ataaagctta tttaacaatc ttaattttac
     3721 tatcttccat taatagggca caaatagcac aaattaacca ggacgaacga gaacaagaaa
     3781 tataacgttc cgaccacaat ggtgtaaact tcttcttata cttgtgtaaa ccattaaatg
     3841 agtagaaatg actaccgaaa tgatagacaa gatacgccat tctctcttta gcaaaacttg
     3901 tttcaacgcg accaactcct gataaaggtg ccatccccaa atcaaaatag tggtatccct
     3961 tctcttgata ataagagaat aatgataaaa agaggaaatc cataacgcca ttcggaattt
     4021 tctgtttatc gtgacgcatt aaatcaatag agataatact cttttcatag tttggcataa
     4081 tattagcaaa agcaacaact tcgtgttcag catttttcac caaagctatt ggggcttgtt
     4141 ggaaataatc tttattaaaa tatcctagtg agaaaccttt ttcaggacgt ccttctaacc
     4201 aagtattaga aatctcttcc aaactattta gtagctcttg actatgtggc gattgtacaa
     4261 cttcgaaata gaaaccatcc ttttcaactc tatttagggc atttctgaaa ggtttgtact
     4321 tattcccttt aagagtaaac gtttctaaat taactaaagc atcctcacca actttcataa
     4381 agtcaaaacc atactcatgt aaaagtagtg tcaacttctg tccaatactg taaaaaacaa
     4441 ggtcatagtc tagcttatca gcatcatcaa taaacgattc aatagcttca cgaatataag
     4501 tgtcatcacc ggctggttcc cccataataa gacatttatt attgacaatt acaaattgga
     4561 acgcaacgca atcttctcca tttttttggt accagtagag ccttttatca tttaaaaagg
     4621 ctaaaccgct atccgaagaa ccaccgtaag cttgaagtag ttttttataa cgatccacat
     4681 tgaacacatc accaatctga caagattttc cttgtaaaat cttaaccaaa cataaataaa
     4741 cgagagtaag aatcaacgta gccaatgcta tcggctccca tgctatgata taatgcaggc
     4801 gttcaatact accacctgta atatgagccc tgatagggaa tagtagtcct gcaatataaa
     4861 atagaactcc cattaaacta acgataatga ttccatcctt aatacgctct tcccagctat
     4921 aaataaattg ttttttatag agagttggct taatgactaa taagcctaac aatagtaaaa
     4981 taaaccagaa agatagtcgc caactaatat gacctaaatt aagataaaac aatgtcaaag
     5041 taatccagat aatagcaatt ggaaaagcat ttttcacttt ttgatcaata gttctagcta
     5101 agagaataaa acaaaccccc agcaataaac ctggaaactg ccataacatt tgttcttgta
     5161 atgggtccaa gcctagcttc tgcaaccaca taatataagt aatattttca aaaaatgctg
     5221 ttgaaaatat taagaatgca cctaaaatac gcatcaaatg gctcaccatg gtttgtagaa
     5281 cagttgatac taactctttc gggacatttt cataacgttg atttatttga ctacctaaat
     5341 aatggataaa gaaatagata cctgcaaaga atggaataat atagtaggct aaacgataaa
     5401 gtaataacca tgcaaccaca gtttctttag gtagtccctc ggcagcaaac cctgtaaata
     5461 gaactaattc aaaacttcct aatccaccgg gaataaggga tacaatcccg acggcacaac
     5521 caatacaaaa tagtggtaaa attttataaa ctggtagatg aatgcccatt aaataaccga
     5581 taattataaa cgctgcggct gcacatcccc attcaaaaaa agaaacaata cctaatttta
     5641 tacgagtact agatggcata tctccgaaat aatggcttcc tttatgacca gaaatccaat
     5701 aaataacagg aaaatacata ctagcaccaa ttaataccaa ataatagtaa tcaacactag
     5761 ctttggcatg aaaaatatgg ctcatgatta aggcaatcac actaataaat gacagaccag
     5821 aaataagata gggtaaaaaa cgagtcactt ctcttaggtc actcttctct tgaccttttt
     5881 taccataaaa agccatgcgc aacccaatat cgattaagcc accgaatcct gacaagttat
     5941 taaaagtgtt gatacaccaa ctagtctgta aaatatacct tttgctttta tctgtacgta
     6001 aaagtccatt cagtacaaag tcatatccgg ttgtcggtaa gacagcgata acccccacaa
     6061 ggataattaa gaaaagattc attggagata attgcccaaa aacactcttt agttgctcaa
     6121 cagaaatagt tcgtttcaaa tgaatcattt caacaataat aagcactaaa acagatataa
     6181 aaaatacaat tttaatcaca gaagtcagtg atttgacttt ttcaattagc tttttcaaca
     6241 atttctcctt tcaacgtatc gttgataaac ttttttgcaa taatattaaa aacatgggaa
     6301 tcttgcttaa taatgtgatg cccaaagcca actaaagaat aaaactcccc ccttggaaaa
     6361 taagaagcaa gtttcttaga atgatttaac ttaattatgt ccttatttcc aaccaaaacc
     6421 attacaggag ttgacacatg ctgtaaatca gctggactaa tcttcaaatc ctccaacata
     6481 agcgaaataa cttgagcttt ttgcctcata tacggaaaga gtttccctaa atagtgaagg
     6541 aatttatagg caatccttac taaaagaata tcccaccatc gctgaccatg aatagtcagg
     6601 ttccctgaat taagcaaaag ccctctaacc atatctggaa acatcgtttg aaaaactaaa
     6661 gctaaattag caccatcgct atggcctacc aatataactt tatcaatctc taaatgaact
     6721 aagatatcct ttaagtcaac tgctatttgc ctgaaactaa tggtatttag ctttgcatga
     6781 cttttgccat gccctctact atccataaca ataacttggt aatacttaga aaaatatgct
     6841 atttgcttat caaaatagcg actacttaag ctattgccat gtaaaaaaac aatcggttga
     6901 ccttgaccaa cgacacgata ataaatatct gaaccatctt tagtatgtaa ccagttctca
     6961 gtcataacca aagtcacctc tctttgaatt catagttata ttatactatt tttaaagaca
     7021 aattacttaa actacctgta ttatgagaaa aaaatgatta ctaaaagccg
//
LOCUS       DATRQA010000102         6864 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.102, whole genome shotgun sequence.
ACCESSION   DATRQA010000102 DATRQA010000000
VERSION     DATRQA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.102"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(29..581)
                     /locus_tag="VBD27_001539"
                     /pseudo
     CDS             complement(29..581)
                     /locus_tag="VBD27_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            892..1146
                     /locus_tag="VBD27_001540"
     CDS             892..1146
                     /locus_tag="VBD27_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEO7025542.1"
                     /translation="MSYEQEFLKDFEEWLQSQISINQMAMDSAKKVLEEDKDERAADA
                     YIRYESKLDAYRFLQGKFNNYHNQKSFHDLPDGLFGQRHY"
     gene            1255..1566
                     /locus_tag="VBD27_001541"
     CDS             1255..1566
                     /locus_tag="VBD27_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025543.1"
                     /translation="MAKRIKPNKKRELLKAKGINWKDVRLSQTLQEVASPKKKAPEAP
                     KAVDLLEGLTANKQDVLKEAGLVSLEAFAKVSEADVLALKGIGPAAIKQLVDNGAVFA
                     K"
     gene            1834..2403
                     /locus_tag="VBD27_001542"
     CDS             1834..2403
                     /locus_tag="VBD27_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7025544.1"
                     /translation="MQILEDFDGKALPKLETDRLILRQRTVGDVPAMFDYVCLEEVAY
                     PAGLSPIASLEDEYDYFENRYYQNLEKAKLPSGYGITVKGSDRIIGSCDFNHRHEDDV
                     FEIGYMLHPDYWGHGYMTEAVAALIEVGFTLLNLHKIEIRCYDYNKQSRRVAEKLGFT
                     LEATIRDRKDNQGNRCVNLIYGLLRSEWE"
     gene            2501..3085
                     /locus_tag="VBD27_001543"
     CDS             2501..3085
                     /locus_tag="VBD27_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7025545.1"
                     /translation="MIKILGEIVDNQLPVVETNRLLLRQRKLEDAKEIFEFVKLDEVT
                     YPAGFPAVKSLEEEITYIQEIYPTNLEKEKLPSGYAITLKGDDKVIGSVDFNHRHEDD
                     IFEIGYLLHPDYWGQGIVPEAASVLVEIGFTLLGLHKIELGCYDYNKQSQAVARKLGF
                     TLEANIRDRRDAQGQRCGDMRFGLLRSEWEKKRR"
     gene            3082..3648
                     /locus_tag="VBD27_001544"
     CDS             3082..3648
                     /locus_tag="VBD27_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7025546.1"
                     /translation="MNIIIIGAQASGKMTIGQEIAKQTGMTLFLNHDSIDFVLRFMPW
                     SPDSIALTESIRFKFFETFAKTGQEMIFTIVIDFNDSRDVAFLEKIQTVFQSHNQEVL
                     FVELETELSERLKRNRTENHLKHKPSKRDIKWSENDICSTMDYAIFNPEVAPEALTYY
                     YKINNTCLTATETAYLIIQKINQIKEKN"
     gene            3648..6299
                     /locus_tag="VBD27_001545"
     CDS             3648..6299
                     /locus_tag="VBD27_001545"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO7025547.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     ISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYNKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGQNVILPIINKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFAVGQRHNLPQVNVMNDDGTMNELADEFNGMDRFEARKAVVAKLESLGNLV
                     KIEKMTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHNLNETVGKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDEKVVTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFGQYADSSIVTAAYPTVNPAFENAAAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILVKTSDSELESFFKDNSNYIKRFTNPETLEISSAIATPELAMSS
                     VITGAEIFLPLADLLNVEEELARLEKELAKWQKELDMVGKKLGNERFLANAKPEVVQK
                     EKDKQADYQAKYDATQERIVEMQKLVK"
     gene            complement(6517..>6864)
                     /locus_tag="VBD27_001546"
     CDS             complement(6517..>6864)
                     /locus_tag="VBD27_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025548.1"
                     /translation="LPQQFITKYDYEFVYKLSKVLAQYTSRNKVGSSYTAHSVIEEIY
                     LYLIAKESILYFESLDENSHLQLKELLDYNDEWPFDIFDDMDSYTFLYTDIYIEEDSP
                     YHFKNWFVPQFYL"
BASE COUNT         2189 a         1217 c         1423 g         2035 t
ORIGIN      
        1 ttatttttcc aaattgatat tctgaacttt aaaaactatt ttgtaagaat aaattttgcc
       61 aatcatctat ttttagagga gcaacgttaa cattcatagt atgtctaagt tgatcgatca
      121 gcttggcagt gtcttcatct tgataagaaa catcatgtgt gagtaaacgt ccaccaggtt
      181 ttaggacacg taagtactct tttatggctt tctccttagc tgaattgtta agcatcgtta
      241 acattgcttc gttgataacg ataaactatt atcaggaaag ggcagtctga gtgcatttcc
      301 ctgaataact tcaataaatt cttctaattg ggcctttttg acattttctt tggccttttc
      361 tattacttta ctatctagat caagcgctgt aatgtgacag gaaaatgctt tagcgatatc
      421 gatggaactg gtacattgat tacaagctac ttctagaaca cgactgtcag cactaaagtg
      481 tccttttttg ataagccaat tcgttgcttg gacacctcct ggtcttaagc gttttttcct
      541 agtcgtgcta agaattgatg tccaatttct ttatgtggca ataagatcac ctctttcttt
      601 taatagtaat atcatttttt cttaattata acacatagat gctaagataa caaggagtaa
      661 tagtgattga atggtataag tgatgaattt tagagagcgt tttcttttaa ctaggggtgt
      721 gatgccaaaa ctagaatggc atagagaacc atacatctca aaaggtcttc cttagataaa
      781 actagttgtt tttatatctg tcagaaaaat cataatttat ttttgatata atgatttttt
      841 gagtatttaa tttatatgtt ataatgaaaa aaactaaaaa agaggaatat catgtcatac
      901 gaacaagaat ttttaaaaga ctttgaagag tggttacaat cgcaaataag tatcaaccaa
      961 atggcaatgg acagtgcaaa gaaagtttta gaggaagata aggatgagcg agcagcagat
     1021 gcgtatattc gttatgagag taagttagac gcttatcgct ttttgcaagg gaaatttaat
     1081 aactatcata accaaaaatc tttccatgat ttgcctgatg gtctttttgg acaacgtcat
     1141 tattaaaagg tttatcgcta gaaagcttgt caaataggca tttctatgat acattgttat
     1201 agtagttttt agatactatg atagatttca taaatgagga gaaagcatta tacaatggca
     1261 aagcgtatta aaccgaataa aaaaagagag cttttgaaag ctaaagggat caattggaag
     1321 gatgttcgac tatctcaaac tttacaagaa gtcgcatcac cgaaaaaaaa ggctcctgaa
     1381 gcgccaaagg cagtcgatct tttagaaggt ttaactgcta ataaacaaga tgttttaaaa
     1441 gaagctggcc ttgtcagcct tgaagcgttt gcgaaagtaa gtgaagcaga tgttttagca
     1501 ctaaaaggta ttggtccagc agctattaaa cagttagttg ataatggtgc tgtttttgca
     1561 aaatgatttg attcaccttg cgaaagtagt cttattttgc tagaataaat taacttagag
     1621 gtgttttgaa atcccatttg tcacaggaag cggtcatact gagaaatccg cacaaatgtc
     1681 aaagcttgtt gctaagtcca ataattgaaa caaaacgagt tgcggatgtt atcatccgaa
     1741 tttgggtggt accgcggaaa tcgtaatacg aaattcgtcc ctgtcagaat tgacagggat
     1801 tttttgtatt taggagtgag gagattgaag tatatgcaga ttctagaaga tttcgatggc
     1861 aaagcattac caaaactaga aacggatcga cttatcttgc gtcagcgaac ggtgggggat
     1921 gtcccagcca tgtttgatta cgtttgcttg gaagaggttg cctatccagc agggctcagt
     1981 ccaatagctt ctttagaaga tgagtatgat tattttgaaa atcgttacta tcaaaatctg
     2041 gaaaaggcaa aattaccatc tggttatggt attaccgtta aaggaagtga ccggattatc
     2101 ggctcttgcg actttaacca ccgccatgaa gacgatgttt tcgagattgg ctacatgctt
     2161 catcctgact attggggtca tggttatatg acagaagctg tcgcagcctt gattgaagtc
     2221 ggttttaccc tcctcaatct ccacaaaatc gaaatccgct gctatgatta caataagcaa
     2281 agccggcgcg tggcagaaaa actaggcttc acccttgaag ccaccatccg tgatcgtaag
     2341 gataatcaag gtaatcgctg tgtcaatttg atatatggtt tgctgaggag tgagtgggag
     2401 taattattct actcttcata gaagttacta gatagactaa tttggctgtg cagggtactt
     2461 agctaactca tcaataatat cgtagatatg gaggagacaa atgattaaga tacttggaga
     2521 aatcgtagat aaccagctac ctgttgtgga aacgaatcgt ctgttgttgc gtcaacgcaa
     2581 gttagaagat gctaaggaga tatttgaatt tgtcaaactt gatgaggtga cttatccagc
     2641 aggttttccg gctgttaaaa gcttggaaga agagataacc tatattcaag agatttatcc
     2701 aactaatctt gaaaaagaaa agttaccctc tggttatgct attactctaa aaggcgatga
     2761 taaggtaatt ggttcagtag attttaacca tcgccacgaa gacgacattt ttgaaattgg
     2821 ctacctgctc caccctgatt attggggaca aggcattgtg ccagaagcag cttcagtcct
     2881 tgtggagata ggtttcacac tgctaggact acataagatt gagctgggat gctatgacta
     2941 taataagcaa agtcaggcgg ttgcgcgcaa attaggtttt actttagagg ctaatattcg
     3001 tgatcgaaga gatgcccagg ggcaacgttg tggcgatatg cgctttggtt tactgcgaag
     3061 cgagtgggag aaaaaaagaa gatgaatatt attatcatcg gagcacaagc ttctgggaaa
     3121 atgacaattg gtcaggaaat cgcaaaacag acaggcatga ccttattcct taaccatgat
     3181 tctatagatt ttgttttacg cttcatgcca tggtctccag atagtattgc tctaacggag
     3241 tctattcgct ttaaattttt tgaaacgttt gctaagactg gtcaagaaat gatttttact
     3301 attgtaattg atttcaatga ttcaagagat gtagcatttc tagaaaagat tcaaacagtt
     3361 tttcaatctc acaaccaaga ggtattattt gtagaattgg aaacagagct aagtgagcgt
     3421 ctcaaacgaa atcgcacaga aaatcacctc aagcataagc cttcaaaacg tgatatcaaa
     3481 tggtcagaga acgatatttg ttcaaccatg gattacgcta tctttaatcc tgaagtagcc
     3541 ccagaagcgc ttacatatta ttataaaatc aataatactt gcttaactgc tactgaaaca
     3601 gcatatctta ttattcaaaa aataaatcaa atcaaggaga aaaactaatg tcaaaagaac
     3661 tatcaccaaa atacaatcct gccgaggttg aggcaggccg ttatcaaact tggcttgacc
     3721 aagatgtttt caagccatca ggggatgctg aggctaagcc ttattctatt gtgattccac
     3781 caccaaacgt tacgggtaaa ttgcacttgg gtcacgcttg ggatacaact ctacaagata
     3841 tcattattcg tcaaaagcgt atgcagggct ttgatacact ctggcttcca ggtatggacc
     3901 acgctggtat agccactcaa gctaaagttg aggagcgcct tcgtgagcaa ggcatttcac
     3961 gttatgatct tggtcgtgaa aaattcctag ataaagtttg ggaatggaaa gacgagtatg
     4021 cagcaactat taaatctcag tgggggaaaa tgggtctttc ggttgattat agccgtgagc
     4081 gttttaccct tgatgaggga ctttcaaaag cggtaagaaa agtctttgtt gatttgtaca
     4141 ataaaggttg gatctatcgt ggggagttca tcattaactg ggatccagca gctcgtacag
     4201 ccctttcaga tattgaagtt attcacaagg atgttgaggg tgctttctat catatgaatt
     4261 atatgctgga agatggctca cgcgccctag aagtagcaac aactcgtcct gagactatgt
     4321 ttggagatgt ggctgtagcg gtaaatccag aagatccacg ttacaaggat ttgattggtc
     4381 aaaatgttat ccttcctatc atcaataaac ctattccaat cgtcgctgat gaacacgctg
     4441 atccagaatt tggaacaggt gtggttaaga ttacgccggc tcacgaccct aatgacttcg
     4501 ctgtaggtca acgccacaac ttaccacaag ttaacgttat gaacgatgat gggactatga
     4561 atgaacttgc ggatgaattt aatggtatgg atcgtttcga agctcgtaag gcagtagtcg
     4621 ctaaattaga atcgttagga aacttagtaa aaattgaaaa aatgactcat tctgttggtc
     4681 attcagaacg aacaggcgtt gtggttgaac cacgtttatc tactcaatgg ttcgtcaaga
     4741 tggatcaatt ggctaagaat gccattgcca accaagatac agaagacaag gtagagtttt
     4801 atccaccacg atttaacgat acttttatga gctggatgga aaatgtccat gactgggtta
     4861 tctctcgtca attatggtgg ggacaccaaa ttcctgcatg gtacaacgtc aatggtgaga
     4921 tgtatgttgg agaagatgcc ccagagggtg acggatggac acaggatgaa gacgttctag
     4981 acacatggtt tagttctgct ttatggccgt tttcaacaat gggatggcca gacaccgaag
     5041 cagctgactt caaacgctac ttcccaacgt caaccttggt aactggatac gatatcatct
     5101 tcttctgggt atcgcgcatg attttccaat ctcttgaatt tacaggacgc caaccattct
     5161 caaatgttct tattcatgga ctcatccgtg atgaagaagg acgtaaaatg tctaaatcac
     5221 ttggcaatgg gattgatccg atggatgtca ttgagaaata tggtgcggat gctcttcgtt
     5281 ggttcctctc aaatggttca gcccctggtc aagatgtgcg tttttcatac gaaaaaatgg
     5341 atgcttcgtg gaatttcatt aataagattt ggaacatttc acgctatatc cttatgaata
     5401 acgaaggttt aacgttggat caagccagag aaaatgtgga aaaagttgtt aatagtcaag
     5461 ttggaaatgt aacagaccgt tggattctcc acaatttaaa tgagactgtg ggtaaagtta
     5521 ctgaaaactt tgacaagttt gaatttggtg ttgctggtca catcctttac aactttatct
     5581 gggaagagtt tgctaattgg tacgttgagt taacaaaaga ggttctttat agtgacaatg
     5641 aagatgagaa agttgttaca cgttctgttc ttctttatac cttagatcaa atcttgcgtc
     5701 ttcttcatcc aatcatgcca ttcgtgacag aagaaatctt tggtcagtat gcagatagct
     5761 caatcgtaac agctgcttac cctacggtta acccagcctt tgaaaatgct gctgcccaca
     5821 agggagttga aagcttgaag gacttgattc gttccgtgcg taatagccga gcagaggtca
     5881 atgttgctcc atctaaacca atcactatct tggttaagac aagtgacagc gaactcgaaa
     5941 gcttcttcaa agacaatagt aactacatca aacgtttcac aaacccagaa acacttgaaa
     6001 tcagctcagc tattgccaca ccagaacttg ccatgtcatc agtcatcaca ggagctgaaa
     6061 tcttcctccc acttgctgac ctcctcaacg tagaagaaga gttagcacga cttgaaaaag
     6121 aactagccaa atggcagaaa gaactggaca tggtaggcaa aaaactcggc aacgaacgct
     6181 tcctcgccaa cgccaaacca gaagttgtcc aaaaagaaaa agacaaacaa gccgactacc
     6241 aagctaaata cgacgcaacc caagaacgga ttgtagagat gcaaaaactt gttaaatagt
     6301 taaaatcatc cattacttcc gcagagtcct gccgctagac catcagtcta agcgacaggg
     6361 ctttttgcta tattcaaaga ttgtgaaaaa ttaatatata tgatgcctac ttcttgcgtc
     6421 tgaaatacaa gaagtgaaaa tgatttggcc ataaagaaaa acaggcagaa acccaaatga
     6481 ttaatgccta tttcttatat tttttcgtca attattttat aaataaaatt gaggtacaaa
     6541 ccagttttta aaatgataag gtgaatcttc ttcaatatag atgtctgtat ataaaaaggt
     6601 atagctatcc atatcatcaa aaatatcaaa aggccactca tcgttataat ctaataattc
     6661 cttaagttgt agatgggagt tttcgtccag agattcaaaa taaagaatgg attcttttgc
     6721 aattaaatag agataaattt cctctattac actatgcgct gtataagatg aaccaacttt
     6781 atttcttgat gtatattgag ccaaaacttt gcttaattta taaacaaact cataatcata
     6841 ttttgtgata aattgttgag gcaa
//
LOCUS       DATRQA010000103         6726 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.103, whole genome shotgun sequence.
ACCESSION   DATRQA010000103 DATRQA010000000
VERSION     DATRQA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.103"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..519
                     /locus_tag="VBD27_001547"
     CDS             <1..519
                     /locus_tag="VBD27_001547"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO7025549.1"
                     /translation="KLLRKEVLITLKSQPQAYKSEVDSKLLEAFIKTKAYQNSCVIAT
                     YLSFDYEYNTQLLIKQALCDGKRVLVPKTYPKGKMIFVDYQKDNLRATPFGLLEPVND
                     RAVEKASIDLIHVPGLIFNNKGFRIGYGAGYFDRYLSDFEGDTISTIYRCQRQDFVEE
                     KHDVAVKEVLCL"
     gene            510..1187
                     /locus_tag="VBD27_001548"
     CDS             510..1187
                     /locus_tag="VBD27_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO7025550.1"
                     /translation="MSMKKFAKEYPTTVLLVGLTTLVFLLMQLTYGSQAESSQVIFQF
                     GGIQGDYLKAYPTNLWRLISPIFVHIGWEHFLLNGLALYFVGQMGESIWGSLRFLILY
                     ILSGLMGNIFTLFFTPHVVAAGASTSLFGVFSAIAIAGYFGKNPYLKQVGKSYQVMIL
                     LNLFFNIFTPGVSLAGHVGGLVGGVLVAIFLTKQNGSLLFKTWQSILALMIFIIVSIS
                     LIGLSLV"
     gene            1319..2362
                     /locus_tag="VBD27_001549"
     CDS             1319..2362
                     /locus_tag="VBD27_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO7025551.1"
                     /translation="MNRKIIGLTLLSLSVLTLTACGNRSDKSANKSDIKVAMVTNQGG
                     VDDKSFNQSAWEGLQKWGKKKGLTKGNGFDYFQSSNESDHANNLDTAASSGYNLIFGI
                     GFGIHDTIEKVSENNKDVKYVIVDDIIKGKENVASVTFADNEAAYLAGVAAAKTTKTK
                     TVGFIGGMEGVVVKRFEAGFKAGVKSIDPAIKVAVSYAGSFTDAAKGKTIAATQYATG
                     VDVIYQAAGGTGAGIFSEAKTENETRKESNKVWVIGVDRDQSQEGNYVSKDGKKANFV
                     LASTIKEVGKSLQSVAELTEKKQYPGGKVTVFGLKDSGVDIKEHQLSSEGSVAVKKAK
                     EGIVSGKIQVPMK"
     gene            complement(2453..3352)
                     /gene="galU"
                     /locus_tag="VBD27_001550"
     CDS             complement(2453..3352)
                     /gene="galU"
                     /locus_tag="VBD27_001550"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO7025552.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNELLKLVDETTGIRLHFIRQ
                     SHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNNKVIPLTKQLINDFEATHASTIA
                     VMEVPHEDVSAYGVIAPQGEGVNGLYSVNTFVEKPSPEEAPSNLAIIGRYLLTPEIFN
                     ILETQKPGAGNEIQLTDAIDTLNKTQRVFARKFTGDRYDVGDKFGFMKTSIDYALQHP
                     QVKDDLKKYIIDLGKSLEKTSKK"
     gene            complement(3389..4405)
                     /locus_tag="VBD27_001551"
     CDS             complement(3389..4405)
                     /locus_tag="VBD27_001551"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO7025553.1"
                     /translation="MTKQKIAVLGPGSWGTALAQVLNDNGHEVRLWGNVVEQIEEINT
                     NHTNQRYFKDITLDSKIKAYTNLEEAINNVDSILFVVPTKVTRLVAKQVASLLKHKVV
                     IMHASKGLEPGTHERLSTILEEEISEQYRSDIVVVSGPSHAEEAIVRDITLITAASKD
                     IEAAKYVQKLFSNHYFRLYTNTDVVGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     TRGLAEITRLGVQLGADPLTFSGLSGVGDLIVTGTSVHSRNWRAGDALGRGEKLEDIE
                     KNMGMVIEGISTTKVAYEIAQNLNVYMPITEAIYKSIYEGANIKDSILDMMSNEFRSE
                     NEWH"
     gene            4575..4904
                     /gene="rnpA"
                     /locus_tag="VBD27_001552"
     CDS             4575..4904
                     /gene="rnpA"
                     /locus_tag="VBD27_001552"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO7025554.1"
                     /translation="MKKTYRVKSDKDFQMIFSRGKNVANRKFVIYYLEKEQKHFRVGI
                     SVSKKLGNAVVRNAVKRKIRHVLLSQKTALQDYDFVVIARKGVEELDYQALEKNLIHV
                     LKIAGLI"
     gene            4917..5732
                     /locus_tag="VBD27_001553"
     CDS             4917..5732
                     /locus_tag="VBD27_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO7025555.1"
                     /translation="MKKKLKTFSLILLTGSLLVACGRGEVSSHSATLWEQIVYAFAKS
                     IQWLSFNHSIGLGIILFTLIIRAIMMPLYNMQMKSSQKMQEIQPRLKELQKKYPGKDP
                     DSRLKLNDEMQSMYKAEGVNPYASVLPLLIQLPVLWALFQALTRVSFLKVGTFLSLEL
                     SQPDPYYILPVLAALFTFLSTWLTNKAAVEKNIALTLMTYVMPFIILVTSFNFASGVV
                     LYWTVSNAFQVFQILLLNNPYKIIKVREEAVRVAHEKEQRVKRAKRKASKKRK"
     gene            5740..6561
                     /locus_tag="VBD27_001554"
     CDS             5740..6561
                     /locus_tag="VBD27_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEO7025556.1"
                     /translation="MVLFTGATVEEAIEKGLQELNISRLRAHIKVVSREKKGFWGFGK
                     KPAKVEIEGITDEVTDINESVALKNIKNVPSSVDVVEEYIEEVDETLEKEDVSQPELP
                     KIDDKNVVTTSEAIEKIDLLPNIEVAAAQVTKYVENIIYEMDLDATIETTTSKRQINL
                     QIETPEAGRIIGYHGKVLKSLQLLAQNYLHDRFSKSFSVSINVHDYVEHRIETLIDFS
                     KKIARRVLETNEPYHMDPMSNSERKTVHKTIATIEGVESYSEGNDPNRFVVVTKK"
BASE COUNT         2247 a         1041 c         1285 g         2153 t
ORIGIN      
        1 aaattattaa gaaaagaagt tttgataacc ttaaaatctc aacctcaagc ttacaaatca
       61 gaagttgact ctaaactttt agaggctttt ataaaaacta aagcttatca aaatagttgt
      121 gtaattgcta cttacctatc ttttgactac gaatacaata cacaacttct tattaaacaa
      181 gctttatgtg atggcaaacg tgtccttgtt cccaaaactt atccaaaagg taaaatgatt
      241 tttgtggact atcaaaaaga taacttgagg gcaacaccgt ttggcttatt agaacctgtt
      301 aatgatagag ctgttgaaaa agctagtata gacttaattc atgtgcccgg acttatcttt
      361 aacaataaag gttttcgtat aggttatggt gcaggttatt ttgatcgcta tttgagtgat
      421 tttgaaggtg atactattag tactatttat aggtgtcaga gacaggattt tgtagaagag
      481 aagcatgatg tagcggttaa ggaggtatta tgtctatgaa gaagttcgct aaagagtatc
      541 cgacgacagt actcttggtc ggtctgacaa cccttgtttt tttattgatg caactaactt
      601 atggaagtca agcagagtct agccaagtta tttttcaatt tggtggaata caaggtgatt
      661 atctaaaagc atatccaaca aatctatgga gactaataag tcctatcttt gttcatattg
      721 gttgggaaca ttttttatta aatggtttag ctctgtattt tgtgggacaa atgggggaaa
      781 gtatttgggg aagtttgaga tttctaattc tctatatttt atctggctta atgggtaata
      841 tttttacgct attttttact ccacacgtgg tggcagcagg agcctcaacg tctttatttg
      901 gtgttttttc tgctatagca atcgcaggtt attttggaaa aaatccttat ttaaaacaag
      961 ttggaaaatc atatcaagtg atgatcttgt taaatttatt ttttaatatc ttcacacccg
     1021 gagtgagttt agcaggccat gttggtggtt tagttggtgg agtattggtg gctatttttt
     1081 tgacaaaaca aaatggttct ttattattta aaacttggca aagtatttta gctttaatga
     1141 tatttattat tgtatcgatc agtttaatag gtctatcgtt ggtataattc tacggctata
     1201 gtctctaaaa ccaaactttc tttgggaaaa atagaataaa gtttgctaaa tgattttttt
     1261 atgttatgat agaaatctgt ttttatgtga ctataactta ttaggaggtt cattctcggt
     1321 gaacagaaaa attattggct taactctact atctttatct gtactaacat taacagcatg
     1381 tggtaatcgt tcagataagt ctgcaaataa atcagatatc aaagttgcga tggtaactaa
     1441 tcaaggtgga gtagacgata aatcgtttaa tcaatcagct tgggaagggc tacaaaagtg
     1501 gggaaaaaag aaggggttaa caaaaggtaa tggctttgat tatttccagt catctaatga
     1561 atcagatcat gctaataacc ttgatacggc agcctctagt ggctataatt taatttttgg
     1621 tattggattt ggtattcatg atactattga aaaagtatca gaaaataata aagatgttaa
     1681 atacgttatt gtagatgata tcattaaagg taaagaaaat gtagctagtg ttacttttgc
     1741 agacaatgaa gctgcttact tagctggagt tgctgcagct aagacaacta agacaaaaac
     1801 agttggtttt atcggaggga tggaaggcgt cgttgttaag cgatttgaag caggatttaa
     1861 ggctggagta aaatcaattg atccggctat taaagttgcg gtatcttatg caggctcatt
     1921 tacagatgca gctaaaggta aaacgattgc tgccacacag tatgcaacag gtgttgatgt
     1981 tatttaccag gctgctggtg gtacaggagc aggaattttt agtgaggcta aaactgaaaa
     2041 tgagacccgc aaggaatcta acaaagtctg ggtaattggt gttgacagag atcaaagtca
     2101 agaaggcaat tatgtttcaa aagatggtaa gaaagcaaac tttgttctgg catctactat
     2161 aaaagaggtg gggaaatctc tccaatctgt agcagaatta acagagaaaa aacaatatcc
     2221 aggagggaaa gtaacagttt ttggtctaaa ggatagtggt gtagatatta aagagcatca
     2281 attgtctagt gagggatctg ttgctgtcaa aaaagctaaa gaggggattg tttcagggaa
     2341 aatacaagtt cctatgaaat gataaaagtg tggaggaaca ataagttctt cgtcaagtac
     2401 aataaaaaag tttcctaaat aggaaacttt tttattgtac ttgactcata ttttatttct
     2461 tagatgtttt ttctagagat tttcctaaat caatgatata tttcttcaag tcatctttaa
     2521 cttgtgggtg ttgaagagca taatcaatag aagttttcat aaaaccaaac ttgtcaccaa
     2581 cgtcataacg gtcccctgta aattttctag caaaaacacg ttgcgtttta tttaaagtat
     2641 cgatagcatc ggttagttga atctcatttc ctgcaccagg tttttgagtt tctaaaatat
     2701 taaaaatttc tggagttaac aagtagcgac caatgattgc caaattactt ggagcctctt
     2761 cgggagaagg tttttcaacg aaagtgttaa cgctatatag accattaact ccttctcctt
     2821 gtggagcaat aacaccataa gcagaaacat cttcatgtgg aacttccata acagcaattg
     2881 ttgatgcatg agtagcttca aagtcattaa ttaattgttt ggtgagagga ataactttat
     2941 tattggtaat atccattaga tcatcaccta gcataacaac aaaaggttca ttaccaacga
     3001 aggcttttgc ttgtagcaca gcatctccaa gcccacgagg atgactttgt cgaataaaat
     3061 ggagtcgaat gccagttgtt tcatcaacaa gtttgagaag ttcgtttttc cctttctctt
     3121 ttaggttata ttctaattca aaatttgaat caaagtggtc ttctatagaa cgttttgatt
     3181 taccagttac aactaaaata tcttcaattc cagattttag tgcttcttca acaataaatt
     3241 gaatagttgg tttatcaacg attggaagca tttctttagc taaagcttta gtagcaggta
     3301 aaaaacgtgt tcctaaacca gcggcaggga taactgcttt tctgactttc atatatagct
     3361 cctttaaata gtattaatat aaacgatatt agtgccattc attctctgaa cgaaattcat
     3421 tggacatcat atctaaaatg ctatctttga tattagcccc ttcatatatt gatttataaa
     3481 tagcttctgt aatgggcatg taaacattta agttttgggc gatttcgtaa gcaaccttag
     3541 ttgttgagat accttcaata accatcccca tatttttttc aatatcttca agtttctcac
     3601 cacgtcctaa agcatctccc gctctccagt tgcgagaatg aacagatgta ccagtcacaa
     3661 ttaaatctcc aactccggat aaaccactga aagtgagagg gtcggcccct aattgtacac
     3721 caaggcgtgt tatctcagct aaaccacgcg taataatagc tgctttagca ttatcaccat
     3781 aaccaagacc gtgtagtgct ccagcaccaa cagcaataat attttttaga gcaccagctg
     3841 tttctactcc tacgacatca gtgttagtat aaagtctaaa ataatgatta ctaaacaatt
     3901 tttggacata cttagctgct tcaatatctt ttgaagcagc agtaatcaaa gtaatatcac
     3961 gaacgatggc ttcttcagca tgactgggac ctgaaacaac aacgatatca ctacgatatt
     4021 gttcagagat ttcttcttct aaaattgtag aaagtcgttc atgagtgcca ggttctagcc
     4081 ccttagaagc atgcataatt acaactttat gctttaaaag actagccact tgtttagcaa
     4141 caagtcttgt aactttagtt gggacgacga atagaatgct gtcaacatta ttaatcgctt
     4201 cctcaagatt agtgtaggct tttattttgg aatctaaggt aatatctttg aagtaacgtt
     4261 gattagtatg gttagtatta atctcttcta tttgttcaac cacgtttccc cataagcgta
     4321 cctcatgtcc attgtcattt aatacttgtg caagagcagt cccccatgaa ccaggtccaa
     4381 gaacggcaat tttttgtttt gtcatcttat tccttctttc taataataag gcatatagcg
     4441 ctaacattat atcatactct aatacacttt gtcttgttta gtctgttgtc gatgatagta
     4501 gcattagaaa agaattgagt cttttaggtt atttatggta taataaaacc aatttagata
     4561 aatggagcac tattttgaaa aaaacctatc gggtaaaaag tgataaggat tttcaaatga
     4621 ttttcagcag aggtaaaaat gttgctaatc gaaaatttgt tatctactat ttagaaaaag
     4681 aacaaaaaca ctttcgtgtg ggaatctctg ttagtaagaa attaggaaat gctgttgttc
     4741 gtaacgctgt taaaagaaaa attaggcatg ttttactctc tcagaaaaca gcgctgcaag
     4801 attatgattt tgtcgtgatt gcgcgtaaag gtgtagagga attagactat caagcattag
     4861 aaaaaaatct tatacacgtt ttaaaaatag caggactcat ctagaaaggt ataaaattga
     4921 agaaaaaact taaaacattt agcttaatat tgctgacagg ttctttattg gttgcttgcg
     4981 gacgaggaga agtatctagt cattcagcta cattatggga gcaaatagtt tatgcatttg
     5041 ctaaatcaat acaatggctc tcctttaacc attcaattgg tttgggaata atcttattca
     5101 ctctaataat cagagcgatt atgatgccgc tttataatat gcaaatgaaa tccagccaaa
     5161 agatgcagga aattcagcca aggctcaagg aattacaaaa aaaatatcca gggaaagatc
     5221 ctgatagccg tttaaagcta aatgatgaaa tgcaatctat gtataaagct gagggagtta
     5281 acccatatgc aagtgtatta cctcttctaa ttcagttacc tgtcctatgg gctcttttcc
     5341 aagctttgac tagggtttct ttcttaaaag taggaacgtt tttgagccta gaattatcac
     5401 agccggaccc atattatatt ttacctgtac tggcggcctt atttaccttt ttatcaactt
     5461 ggttaactaa caaagcagct gttgaaaaaa atattgcttt aactcttatg acttatgtta
     5521 tgccgtttat catattggta acaagtttta actttgctag tggtgttgtt ttatactgga
     5581 cggtgtctaa tgcttttcaa gtatttcaaa ttctattatt gaacaaccca tacaagatta
     5641 taaaggttcg agaagaagca gtaagagtgg cacacgaaaa agaacaaaga gttaaacgtg
     5701 ctaagagaaa agcaagtaag aaaagaaagt aggaaaaaca tggtattatt tacaggagcg
     5761 actgtagaag aagctattga aaaaggacta caagagttaa acatttcacg attgagggca
     5821 catataaaag ttgtatcacg tgagaaaaaa ggtttttggg gctttggtaa aaagccagca
     5881 aaagttgaaa tagaaggtat caccgacgaa gtaactgata ttaatgaatc ggtagcgtta
     5941 aaaaatatta agaatgtacc gtctagcgta gatgtggtgg aagaatatat agaggaggtt
     6001 gatgaaacgc ttgaaaaaga agacgtatca caaccagaac ttccaaaaat tgatgataaa
     6061 aacgttgtaa ctacaagtga agctattgaa aaaattgacc tacttcctaa tattgaagta
     6121 gcagctgctc aggtgactaa atacgttgaa aatatcatct atgagatgga tttagatgca
     6181 acaatagaaa ctacgacaag taagcgacaa ataaatctcc aaatagaaac gccagaagct
     6241 gggcgcatca ttggttatca tgggaaagta ttaaaatcgt tgcagttgtt agcacaaaat
     6301 tatcttcatg accgcttttc taagagtttt tcagtatcta ttaatgtcca tgactacgtt
     6361 gagcatcgta tagaaacttt aattgatttt tctaaaaaaa tagctagacg tgttttagag
     6421 actaatgagc cttaccatat ggatcctatg agtaacagtg agcgaaaaac tgttcataaa
     6481 acaattgcaa ctatagaggg tgttgaaagt tattctgaag gtaacgatcc taatagattt
     6541 gtagtagtta ctaagaagta aaaagaggtt tcacctcttt ttttcttgcg cccgatctac
     6601 attttcagaa aatgtagaaa aagtatgaaa aaagagttga caaagtaaga gatgatgtag
     6661 taaactaaat aagttgtctc gagagagcag tttctagtag cgcgtagtta acaacttaaa
     6721 aaaagt
//
LOCUS       DATRQA010000104         6636 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.104, whole genome shotgun sequence.
ACCESSION   DATRQA010000104 DATRQA010000000
VERSION     DATRQA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.104"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(51..599)
                     /locus_tag="VBD27_001555"
     CDS             complement(51..599)
                     /locus_tag="VBD27_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7025557.1"
                     /translation="MTESVTVNKKRIKQITQTQEDILTAFGQLLEEYPYEKIQISQIA
                     KKSGYARRTFYRHFDSRDDLLTLFIERLTLNLFKQLGQLEQPTFSQVFQNFFSYWSDY
                     KSLLLILRKNDLLPQFQQSWFRHIDLIELGRGDLSSNTYAQRFAIGGIFSVLIEWIHQ
                     DCQTSIEELTQVSFDIINHLKN"
     gene            691..1340
                     /locus_tag="VBD27_001556"
                     /pseudo
     CDS             691..1340
                     /locus_tag="VBD27_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     gene            1352..2002
                     /locus_tag="VBD27_001557"
     CDS             1352..2002
                     /locus_tag="VBD27_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEO7025558.1"
                     /translation="MSYFRNYWYRFGAILFIILAVILLVFRPDWSMLHYLLYFYFMAL
                     LAHQFEEYQFPGGASPIINYVVYDEEELMDCFPGNTQSIMLVNTIAWLLYIASIAFPQ
                     AYWLGLGVMFFSLTQLLGHGFQMNIKLKTWYNPGLATTVFLLVPIACAYIYQASAEGM
                     LTWGDWLGGFIMLIVCVLTSIIAPVQLLKDKETNYIISPWQMDRFHKVVNFVRIKK"
     gene            2132..3508
                     /locus_tag="VBD27_001558"
     CDS             2132..3508
                     /locus_tag="VBD27_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO7025559.1"
                     /translation="MENHRYEDEGKFQRKMTSRHLFMLSLGGVIGTGLFLSSGYTIAQ
                     AGPLGAVLSYLIGAVVVYLVMLSLGELAVAMPVTGSFHTYATKFISPGTGFTVAWLYW
                     ICWTVALGTEFLGAAMLMQRWFPNVPAWAFASFFALVIFGLNALSVRFFAEAESFFSS
                     IKVIAIIIFIILGLGAMFGLVSFEGQHKAILFTHLTANGAFPNGIVAVVSVMLAVNYA
                     FSGTELIGIAAGETDNPKEAVPRAIKTTIGRLVVFFVLTIVVLASLLPMKEAGVSTAP
                     FVDVFDKMGIPFTADIMNFVILTAILSAGNSGLYASSRMLWSLANEGMLSKSVVKINK
                     HGVPMRALLLSMAGAVLSLFSSIYAADTVYLALVSIAGFAVVVVWLAIPVAQINFRKE
                     FLKENQLEDLSYKTPFTPVLPYITIILLLISIVGIAWDSSQRAGLYFGVPFIIFCYIY
                     HKLRYKKW"
     gene            3513..4457
                     /gene="mmuM"
                     /locus_tag="VBD27_001559"
     CDS             3513..4457
                     /gene="mmuM"
                     /locus_tag="VBD27_001559"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEO7025560.1"
                     /translation="MGRFKELLESKKTLILHGALGTELESRGCDVSGKLWSAKYLIED
                     PAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQVGISESQAEDLIRLTVQLAKAAREQ
                     VWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIDKQGLKNFHRHRIELLL
                     DEGVDILALETIPNAQEAEALIELLAEDFPQVEAYMSFTSQDGKTISDGSAVADLAKA
                     IDVSPQVVALGINCSSPSLVADFLQAIAEQTNKPLVTYPNSGEVYDGASQSWQSSPDH
                     SHTLLENTSDWQKLGAQVVGGCCRTRPADIADLSAHLK"
     gene            complement(4550..6616)
                     /locus_tag="VBD27_001560"
     CDS             complement(4550..6616)
                     /locus_tag="VBD27_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO7025561.1"
                     /translation="MDDIFNSLMGNMGGYNSENKRYLINGREVTPEEFSQYRQTGKLP
                     GQELNNQNTPTNQVSADSVLTKLGTNLTDQARQHLLDPVIGRNKEIQETAEILARRTK
                     NNPVLVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEIISIDISSLEAGTQYRGSFEE
                     NIQNIIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADILKPALSRGELTVIGA
                     TTQDEYRNTILKNAALARRFNEVKVNAPSAQDTFNILMGIRNLYEQHHNVVLPDSVLK
                     AAVDLSIQYIPQRSLPDKAIDLIDMTAAHLAAQHPVTDLKSLEKEIAEQRDKQEKAVN
                     TEDFEEALKVKTRIEELQNQIDNHTEGQKVTATINDIAMSIERLTGVPVSNMGASDIE
                     RLKELGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSFLFVGPTGVGKTELA
                     KQLAFDMFGSKDAIVRLDMSEYNDRTAVSKLIGATAGYVGYDDNSNTLTERIRRNPYS
                     IVLLDEIEKADPQVITLLLQVLDDGRLTDGQGNTINFKNTVIIATSNAGFGNEAFTGD
                     SDKDLKIMERISPYFRPEFLNRFNGVIEFSHLSKDDLSEIVDLMLDEVNQTIGKKGID
                     LVVDENVKSHLIELGYDEAMGVRPLRRVIEQEIRDRITDYYLDHTDVKHLKANLQDGQ
                     IVISER"
BASE COUNT         1755 a         1265 c         1343 g         2273 t
ORIGIN      
        1 atgacaaaaa gagctttgca gtagctgttg agactactgc aaagctcttt ttaatttttt
       61 aaatgattga taatatcgaa ggaaacttga gttaattctt cgatacttgt ttggcagtcc
      121 tgatgaatcc actcaattag aacactgaaa ataccaccaa tcgcaaatcg ctgagcatag
      181 gtattggaag ataaatcacc acgacctaac tctatcaaat caatatgtcg gaaccaggat
      241 tgttgaaatt gagggagtag gtcattcttt ctaagaatga gaagaaggga tttataatca
      301 gaccaataac taaaaaaatt ttggaaaact tggctaaaag taggttgctc caattgacca
      361 agttgcttaa agagatttaa tgttagtctt tcgatgaaaa gggtaagtag gtcatctcga
      421 ctgtcaaagt gtcggtaaaa ggtccttcta gcatagccag atttcttggc aatttggcta
      481 atctgaattt tctcataggg atactcttcc aaaagttggc caaaagctgt taagatatcc
      541 tcttgtgttt gagtgatttg ttttattctc tttttattaa ctgtcacact ttcagtcatt
      601 tgtgcataaa acctccaaag gtattgatat tataggttca cttgattata ctatatggta
      661 tcaaatgtca caaatcttgg aggtcatctt atgaaagcat tgataacaaa atggtacctc
      721 ttttgtccct atcttgcttc tctttttgcc ttagcattat tttttggaaa ttgggattta
      781 cgagtgcagt cactacttat ttcgggactt tttattcaat tacacttttt cgaagaattt
      841 ggtttccctg gaggttttcc gcttatcact atgctagttg aacttaaatc ggttgaaact
      901 gatactagta agtgggactt aaaccatcta tcagcctttt tcgggaatca gtggtttgca
      961 gtgattgttt atcttttgcc gattttttgt cccaatatac catttttaac acttgcagtc
     1021 atgatttttg catttgcaga acttgccatg catcttttct tttttaatct ttccttgaag
     1081 aagtggtata atccaggact tcttacgacc ttagtaggtc ttgtccctgt ttctgtctat
     1141 tatttagctc atgattggaa gttgtattct ggacttgatt ggtttttagg ctgatatgga
     1201 ttgttcttaa ctactttata gcctttcgtt ccccaatcta taagcgttta ggtcgacatt
     1261 caaattacgc atttagtgat gttgatcttt cacgctcaaa accattttta acacattttc
     1321 gtgaaacaca gtttaaatag gaggtattat catgtcatat tttagaaatt actggtatcg
     1381 ttttggagca attttattta ttattttagc agtaatattg cttgttttta gacctgactg
     1441 gtcaatgctt cactatctat tgtattttta ctttatggca cttctagcgc atcaatttga
     1501 agaatatcag tttcccggtg gggcatcacc tatcattaac tatgttgttt atgatgaaga
     1561 agagctgatg gattgttttc caggcaatac tcagtctatt atgttggtta atactattgc
     1621 ttggttgctt tacattgcta gtattgcttt tcctcaagct tattggcttg gattaggagt
     1681 catgttcttt agtctaacgc agctcttggg tcatggtttt cagatgaata ttaaacttaa
     1741 aacttggtat aatcctggtc tagcaacgac agtatttctc ctagtaccaa tagcttgcgc
     1801 atacatctat caagctagtg cagaaggaat gctcacttgg ggagattggc taggtggttt
     1861 tatcatgttg attgtctgtg tactaactag cattattgca cctgtacagc tattgaagga
     1921 taaggagacc aattatatta ttagtccttg gcaaatggac cgttttcata aggtcgttaa
     1981 ttttgtaagg ataaaaaaat aaggttaccg ctatttatga ttcgctgatt ttttgtaata
     2041 gtttttcttt atcatatatg atagctaata cctattcccc aatacgacta gattgtggta
     2101 ggataaaagt attagtttct tggaggttgt tatggaaaat catcgttatg aagatgaagg
     2161 taaattccag cgtaagatga ccagtcgtca tctctttatg ttatcgctag gtggtgttat
     2221 cgggactggg cttttcttga gttcaggtta taccattgca caggctggtc cgcttggagc
     2281 tgtgctgtct tatttgattg gtgccgttgt ggtttatttg gtcatgctat cacttgggga
     2341 attggcggtt gccatgccgg tgacggggtc attccacact tatgccacta agtttatcag
     2401 tcctggaaca ggttttactg ttgcttggct atattggatt tgttggacgg tcgccttggg
     2461 gactgaattt ttaggtgctg ccatgctgat gcagcgctgg ttcccaaatg tgccggcttg
     2521 ggcatttgct tccttttttg cccttgtgat ttttggttta aatgctctta gcgtacgctt
     2581 ttttgcagaa gcagagtctt tcttctcaag tattaaggtt attgctatca ttatctttat
     2641 tatcttgggc ttaggtgcta tgtttggtct agtttccttt gaaggtcagc acaaggctat
     2701 tctcttcact catctgactg ccaatggtgc ctttccaaat ggtatcgttg cagttgtctc
     2761 agtcatgttg gctgttaact atgccttctc tggtactgag ttaattggta ttgcggctgg
     2821 tgaaacggat aatcccaaag aagctgtacc aagggctatt aaaacgacaa tcggtcgctt
     2881 ggttgttttc tttgtactga caattgttgt cctagcttcg ctattgccaa tgaaagaggc
     2941 aggcgtatcc acagcaccat tcgttgatgt ctttgacaag atgggaatcc cttttacggc
     3001 ggatatcatg aacttcgtta tcttgacagc catcctgtct gctggtaact caggtctcta
     3061 cgcatcaagc cgtatgctct ggtcccttgc caatgaaggt atgttgtcaa aatctgttgt
     3121 gaaaatcaat aaacacggtg tcccaatgcg tgctcttctc ttgtcaatgg caggagcagt
     3181 gctgtcgctc ttttcaagta tttacgctgc agacacagtt tatctagcct tggtttcaat
     3241 cgcgggcttt gctgttgttg tcgtatggct agccattcca gtcgcacaaa tcaatttccg
     3301 caaggaattc ttgaaagaga atcaactaga agatttgtct tacaaaacac cttttacacc
     3361 agttctccct tatatcacaa tcattttgct cttgatttcg attgtaggga ttgcttggga
     3421 tagctcacaa cgtgctgggc tttacttcgg tgttccattt attattttct gctatattta
     3481 tcacaaacta cgttacaaga aatggtgatc ttatgggaag atttaaagaa cttttagaga
     3541 gtaaaaaaac tctcatccta catggtgcct tggggaccga gcttgagagt agaggttgtg
     3601 atgtttctgg gaaactttgg tcagccaaat atctgattga ggatccagca gctattcaga
     3661 ccattcatga agattatatt cgtgctggcg ctgatattgt gacgacatca acttatcagg
     3721 cgactttaca aggactagct caagttggta tctctgaaag tcaggcagaa gatttgattc
     3781 gcttgactgt tcagttggca aaagctgcgc gtgagcaagt ttggaaaagt ttgacaaaag
     3841 aagaaaaatc agagagaatc tatcctttga tttctgggga tgttggtccc tacgctgcct
     3901 ttttagcgga tggctctgag tacactggac tctacgatat tgataagcaa ggtcttaaaa
     3961 acttccatcg ccatcgtatt gagcttcttt tagatgaggg tgttgacatt ctggcacttg
     4021 aaaccattcc aaacgctcag gaggctgagg ctcttattga gctacttgcc gaggatttcc
     4081 ctcaggttga ggcttatatg tcattcacct ctcaagatgg taagaccatc tcagatggta
     4141 gtgctgttgc agacttggcg aaagctattg atgttagccc acaggtcgtg gcgctgggca
     4201 ttaactgttc aagtccatcg ttagttgctg atttcttgca agcaatagca gagcagacta
     4261 acaagcctct tgtgacctat ccaaactctg gagaggttta tgatggggca agccaatctt
     4321 ggcaatctag ccctgatcat tcacacaccc tacttgaaaa tacaagtgac tggcaaaaac
     4381 ttggcgccca agttgtcgga ggttgttgcc gaactagacc agctgatatt gctgacctct
     4441 ctgcacattt aaaataagta aagtatcttg aagaaggtta gggtaagttt gatttccttt
     4501 ttcaatccac tagaaaaccc gcttggattc ttccaaacgg gttttgtttt tatctttcag
     4561 aaatgacgat ttggccatct tgcaaattag cttttaggtg tttaacgtct gtatgatcga
     4621 gatagtagtc tgtgatgcga tctcgaattt cttgctcgat gacacggcgc aatggacgta
     4681 ctcccattgc ttcgtcataa cccagttcaa ttaagtgtga tttaacattt tcatctacca
     4741 caaggtcaat tccttttttg ccaattgttt ggttaacttc atcaagcatc aaatctacaa
     4801 tttcgcttaa gtcatctttg cttaggtgag agaattcaat aacaccattg aaacgattta
     4861 gaaattctgg acggaaatat ggagaaattc gttccataat tttcaagtct ttatcgctgt
     4921 cacctgtaaa tgcttcgttt ccaaaacctg catttgaggt tgcaatgata acagtatttt
     4981 taaagttaat agtgtttcct tgaccatcag ttaaacggcc atcatctaga acttgaagga
     5041 gaagcgtgat aacttgcggg tcagcttttt cgatttcatc aagaagaaca atagaataag
     5101 gatttcgacg gatacgttct gttagtgtat tactattgtc gtcataacca acgtaaccag
     5161 cagtcgctcc aattagctta gaaactgctg tacgatcatt atattcagac atatcaagtc
     5221 taacgatagc atcctttgat ccgaacatat caaaagctaa ttgctttgct agctcggttt
     5281 taccaactcc agttggtcca acaaatagaa agctaccaat cggacgatta ccatcatcga
     5341 aaccagcacg atttcggcga atagctctcg caactgcctc aacagcatca ttttgaccaa
     5401 tgactttacc ttttagacga ttgccaagtt cttttaagcg ttcaatatca ctagcaccca
     5461 tatttgatac aggaactcca gttagacgct caattgacat agcgatatcg ttgatagttg
     5521 cagtgacttt ttgtccttcg gtatgattat caatctggtt ttgtaactct tcaatgcgtg
     5581 tcttgacctt aagagcttct tcaaaatctt cagtattgac agctttttct tgcttgtctc
     5641 tttgctcagc gatttctttt tctaaggatt taaggtcagt tacgggatgt tgcgcagcta
     5701 ggtgtgcagc agtcatatca ataaggtcaa ttgctttatc agggagactt ctttgtggga
     5761 tgtattgaat agataaatct acagctgctt tcaaaacaga gtcaggtaaa acaacattat
     5821 gatgttgctc atagaggttt cttatcccca taaggatatt gaaagtatct tgtgcagaag
     5881 gtgcattgac tttaacttcg ttaaagcgac gtgctaaggc agcattcttg agaatggtgt
     5941 tacgatattc atcttgagta gttgcaccta taaccgttaa ctcaccacgt gaaagtgcag
     6001 gcttaagtat atctgcgagc cccttagagc cactatcacc accagttgaa ccggctccta
     6061 atatttggtg tatttcatca aagaagagaa taatatttcc tgtttcctta acttctttta
     6121 tgatattttg aatattttct tcaaaactac ccctatattg ggtgccagct tcaaggctag
     6181 aaatatctat cgaaatgatt tctttatttt tgatagcagc agggacatca ccatttataa
     6241 ttgcttgtgc caaaccttca ataacagctg ttttaccaac acctgcatca ccaaccagaa
     6301 caggattatt tttagtacga cgagctagaa tttctgcagt ttcctgaatt tctttgtttc
     6361 gtccaataac tggatctagc aaatgctgac gtgcttgatc ggttaaatta gttcctaatt
     6421 tagtcaaaac actatctgca gagacttggt ttgtaggagt gttttggtta tttagctctt
     6481 gaccggggag ttttccagtt tgacgatatt gactgaattc ttcaggagta acttcccgtc
     6541 cattgattaa gtatcgctta ttctcactgt tatagccccc catatttccc attagagaat
     6601 taaaaatatc atccatattt ccaaaagggt cacgat
//
LOCUS       DATRQA010000105         6296 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.105, whole genome shotgun sequence.
ACCESSION   DATRQA010000105 DATRQA010000000
VERSION     DATRQA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.105"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..570
                     /gene="dprA"
                     /locus_tag="VBD27_001561"
     CDS             <1..570
                     /gene="dprA"
                     /locus_tag="VBD27_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO7025562.1"
                     /translation="VLLFYQGNIELLSKPKLAVVGARQASQIGCQSVKKIIKETNNQF
                     VIVSGLARGIDTAAHVSALKNGGSSIAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEY
                     FTGEQPLKFHFPERNRIIAGLCQGIVVAEAKMRSGSLITCERALEEGREVFAIPGNII
                     DGKSDGCHHLIQEGAKCIISGKDILSEYQ"
     gene            665..2785
                     /gene="topA"
                     /locus_tag="VBD27_001562"
     CDS             665..2785
                     /gene="topA"
                     /locus_tag="VBD27_001562"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO7025563.1"
                     /translation="MATTTKTSTKKTSKKKSATAKKNLVIVESPAKAKTIEKYLGRNY
                     KVVASVGHIRDLKKSSMSIDFENNYEPQYINIRGKGPLINDLKKEAKKAKKVYLASDP
                     DREGEAISWHLAHILDLDKEDRNRVVFNEITKDAVKNAFVEPRQINMDLVDAQQARRV
                     LDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFQPEEYWTIDGSFKK
                     GTRKFNATFYGLDGKKFKLSNNEDVKTVLKRIKTDEFLVEKVEKKERRRNAPLPYTTS
                     SLQQDAANKINFRTRKTMMIAQQLYEGLSLGTAGHQGLITYMRTDSTRISPLAQNEAT
                     EFITNRFGANYSKHGNKVKNASGAQDAHEAIRPSSVNHTPESIAKYLDKDQLKLYTLI
                     WNRFIASQMTAAVFDTMKVNLTQNGVTFIANGSQVKFDGYMAVYNDTDKNKMLPDMEE
                     GESVKKVNTNPEQHFTQPPARFSEASLIKTLEENGVGRPSTYAPTLETIQKRYYVKLA
                     AKRFEPTELGEIVNSLIVEFFPDIVDVTFTAEMEGKLDEVEIGKEQWQKIIDEFYKPF
                     EKELAKAETEMEKIQIKDEPAGFDCELCGSPMVIKLGRYGKFYACSNFPECHNTKAIT
                     KEIGVICPICQKGQVIERKTKRNRIFYGCDRYPECEFTSWDKPIGRTCPKSNDFLVEK
                     KVRGGGKQVVCSNEKCDYQEEKIK"
     gene            2922..3623
                     /locus_tag="VBD27_001563"
     CDS             2922..3623
                     /locus_tag="VBD27_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025564.1"
                     /translation="MSYIEMKHSYKRYQTGETEIVANNDISFSIERGELVVILGASGA
                     GKSTVLNILGGMDSNSEGEVLIDGKNIANYTIRELTRYRRYDVGFVFQFYNLVPNLTA
                     LENVELASEIVPKALDAQQALENVGLGHRINHFPAQLSGGEQQRVAIARAIAKKPKLL
                     LCDEPTGALDYQTGKQVLAILQKMAQSKETTVIIVTHNTALAPIANRVIHMHDSKISD
                     IVINENPSDIQNIEY"
     gene            3635..6265
                     /locus_tag="VBD27_001564"
     CDS             3635..6265
                     /locus_tag="VBD27_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025565.1"
                     /translation="MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNM
                     QRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLESLKGSQVEFSYFVDTTPQQNSKSYR
                     LYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFSNTGPLT
                     IVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARVTFKDLRLINPF
                     SISYKEKVAKYQEKVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDND
                     SLAMPLSQRQAIQMKIKQDRLSLLKRTKELLKLRHNTQIMESPQIIVYNRTTFPGGQG
                     YNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKALGYSDRQV
                     IFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFV
                     FSLLSIVLPVFVITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTAR
                     NIFRYKQRMIMTIFGVAGSVALLFSGLGIQSSLKQTVNEHFGRIMPYDILLTYNTNAS
                     PPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLPFIYLQEAT
                     TNKSLHLNNKGIIISKKLAQFYHVNTGDFIHLSHSQTLPSRKLKITGVVNANVGHYIF
                     MTKQYYRTIFKKEAKDNAFLVKLTKHKIANNLAEKLLEINGVESLTQNALQLASVEAV
                     VRSLDGSMTILVVVSLLLAIVILYNLTNINLAERKRELSTIKVLGFYNEEVTLYIYRE
                     TIILSTIGVILGTISGTYLHRQMMLLIGSDQILFGEKVSPTTFIIPISVVVIILISLG
                     FIVNHQLKKLNMLDALKSVD"
BASE COUNT         2226 a         1062 c         1117 g         1891 t
ORIGIN      
        1 gttctacttt tttatcaggg taatattgaa cttctatcta aacctaaatt agctgtagtg
       61 ggggcaagac aggcatctca gataggttgt cagtctgtta aaaagattat caaggagact
      121 aacaatcaat ttgttatcgt aagtggttta gcgcgtggca ttgatacagc agcacatgtt
      181 agtgctttaa aaaatggcgg cagtagtata gctgttatcg ggagtggttt agatgtttat
      241 tatccaacgg agaataagaa acttcaagaa tatatgtcat ataatcatct cgtattatca
      301 gaatatttta ccggagaaca acccttgaaa tttcatttcc ccgaacgtaa ccgtattatt
      361 gcagggctgt gtcaaggtat tgtggttgcc gaagctaaga tgagatctgg aagtttaatt
      421 acctgtgaaa gagcattaga agagggacga gaagtttttg ccattcccgg aaatattatc
      481 gatggcaaat cagatggatg ccaccatctt atccaagaag gagctaaatg cattatttca
      541 ggaaaagata tcctttctga atatcagtag tgttttgctt tctactatag gagaagtttt
      601 ctattgacag tttaagaaaa gtgagttatt atgctataag tttacgatat agaaagtgat
      661 acctatggca actacgacaa aaacgtcgac caaaaaaaca agtaaaaaaa aatcagctac
      721 tgctaaaaaa aatctagtta ttgtggagtc tcctgcaaaa gcaaagacta tcgaaaaata
      781 cttaggacgt aactataaag ttgtagcctc agtaggtcat atccgtgatt taaaaaagtc
      841 aagtatgtcc attgattttg agaataatta cgaaccacaa tatattaata ttcgtggtaa
      901 aggacctctc attaatgatt taaaaaaaga agctaaaaag gctaaaaaag tttacctcgc
      961 gagtgacccg gaccgtgaag gagaagctat ttcctggcat ttagcgcata ttttagattt
     1021 agacaaagaa gacagaaacc gtgttgtttt caatgaaatc acaaaagatg ctgttaaaaa
     1081 tgcttttgtg gaacctcgtc agattaacat ggatctcgtt gatgctcagc aagcacgacg
     1141 tgttttagat cgcattgttg gttattctat ctcacctatt ttatggaaaa aagtaaaaaa
     1201 agggctatca gctggacgtg tacaatcagt tgctcttaaa ctaatcattg atcgtgaaaa
     1261 tgagattaag gctttccaac cggaggagta ttggactatt gacggttcct ttaaaaaagg
     1321 aacacgtaaa ttcaatgcca ctttctacgg tttagacgga aagaaattca aattatcgaa
     1381 taatgaagac gtcaaaacag ttctaaaacg tattaaaact gatgaattct tagttgaaaa
     1441 agttgaaaaa aaagagcgtc gtcgtaatgc accattaccg tatacaactt cttcattgca
     1501 acaagatgca gctaataaaa tcaattttcg aactcgtaaa accatgatga ttgcgcaaca
     1561 gctgtacgaa ggacttagct taggcacagc aggtcatcaa ggtctgatta cctatatgcg
     1621 tactgattct acacgtatta gtccgttagc acaaaatgaa gcaacagaat ttattactaa
     1681 ccgttttggt gcaaattatt ctaaacatgg gaataaagtt aaaaatgctt ctggagcaca
     1741 agacgctcac gaagctattc gcccatctag tgttaatcat acacccgaaa gtattgctaa
     1801 gtatttagac aaagatcaac taaaacttta cactcttatc tggaaccgtt ttattgcaag
     1861 ccaaatgaca gcagcagtct ttgacacaat gaaagttaat ttaacgcaaa atggtgttac
     1921 ttttattgct aatggtagcc aagtcaagtt tgatggttac atggctgttt ataacgatac
     1981 tgacaaaaat aaaatgttac cagatatgga ggaaggagaa agtgttaaaa aggttaatac
     2041 gaatcctgaa caacacttca ctcaacctcc tgcaaggttt tcagaagcaa gtctcattaa
     2101 aacacttgaa gaaaacggtg taggtcgtcc ttcaacttat gccccaacgc ttgagactat
     2161 tcaaaaacgt tattatgtta agcttgcagc taaacgcttc gaaccaactg aacttggtga
     2221 aattgttaat agccttattg tagaattttt cccagatatt gttgatgtta ccttcactgc
     2281 cgaaatggaa gggaaactag acgaagttga gattggtaaa gagcagtggc aaaagattat
     2341 cgatgaattt tataaaccat ttgaaaaaga acttgcaaaa gcagaaactg aaatggagaa
     2401 aattcaaata aaggatgaac ctgctggatt tgattgcgag ctatgtggat caccaatggt
     2461 aataaaacta ggacgttatg gaaagtttta tgcgtgtagc aatttccctg aatgtcataa
     2521 cacaaaagct atcactaagg aaataggtgt tatttgtcct atctgtcaaa aaggacaagt
     2581 tattgagcga aaaacaaaac gcaatcgtat cttttatggc tgtgaccgct acccagaatg
     2641 tgagtttaca tcttgggaca aacctattgg acgaacttgc cccaaatcaa atgatttctt
     2701 agtggagaaa aaagtacgcg gtggtggtaa acaagttgtt tgctcaaatg aaaaatgcga
     2761 ctaccaagaa gagaaaatta aataaagtgg aatctgagtg tttagctcag atttttaaaa
     2821 aaattattat tttacataac ttaatttatg tatttatttt tatgggagaa ttaataaaaa
     2881 agtgctataa tataggtgta ttcatagtgt gaggtgccgt tatgtcctat attgaaatga
     2941 agcattccta taagcgttat caaacaggtg aaaccgaaat tgttgctaac aatgatatta
     3001 gtttcagtat tgaaagaggt gagttggtgg ttattcttgg tgcatccggc gctggcaaat
     3061 ctactgttct caatatacta ggagggatgg atagtaatag tgaaggtgaa gttttaattg
     3121 atggtaagaa tattgctaat tataccatca gggaattgac tcgttaccgt agatatgatg
     3181 tcgggtttgt ttttcaattc tataacttag tccctaatct aacagcacta gagaatgtag
     3241 aattggcttc agaaattgtt ccaaaggcat tagatgccca acaggcatta gaaaacgtag
     3301 gtttgggaca ccgtatcaat cattttccag cacagctttc cggtggcgaa cagcaacgcg
     3361 ttgcaatagc acgagctatt gccaaaaaac ctaaactgtt actttgtgat gaacctactg
     3421 gtgctttaga ttaccaaact ggaaaacaag tactagccat cctgcaaaag atggcgcaat
     3481 caaaagaaac aactgtgatc attgttactc ataatactgc cttagcgcca attgcaaatc
     3541 gcgtgatcca tatgcatgat tcaaaaatca gtgatattgt aataaacgag aatccaagtg
     3601 atattcagaa catagaatat tagaggttat acagatggga aagacatttt ggaaagatat
     3661 ctatcgatca attacgactt caaaaggtag attttcatca atcctcttac ttatgatgtt
     3721 aggctcattt gcttttatag gactgaaggt ctcagcacct aatatgcaaa gaacagcaca
     3781 aaattacctt gctcatcatc atgttatgga tattaccgtt ttcaattctt ggggacttga
     3841 caaacatgat caaactgttt tagaaagctt aaaaggatca caagttgaat tttcttactt
     3901 tgtagataca acccctcaac agaatagtaa atcttatcga ttgtattcca atactaaaac
     3961 gatatcaacc tttgatcttg ttaagggacg tctcccttta aataaatcag aaattgctct
     4021 ctcatttcag gaacgaaaaa aatatgcaat aggtgataaa attaatttta aacaagataa
     4081 aaacaaactg ttttcaaata caggtcctct aactattgta ggttttgtta attctaccga
     4141 gatttggtct aagacaaacc ttggtagttc tcaaacaggg gacggcgact tagatagtta
     4201 tggcgtatta gataagacag catttcactc tccagtatat acaatggcaa gggtaacttt
     4261 taaagatctt aggttgatta accctttctc aataagttac aaagaaaaag tagcgaaata
     4321 ccaagaaaag gtatcgcgaa aattaaatat tcacaataaa ataagatata ccaaaacaaa
     4381 aaaagagagc ctacgtaaaa tcgatgagga ggaaaaaagc ctactaaaag ctcaaaaaca
     4441 aatcaatcgg ttagataatg atagcctagc gatgccacta tctcaaagac aagctattca
     4501 aatgaaaatt aaacaagacc gactatctct tttgaaacga acaaaagagc ttctaaaatt
     4561 gagacataac acccaaataa tggaatcgcc tcaaattatt gtctacaacc gtactacctt
     4621 tcctggagga caaggatata acacttttga ctccagtaca aatagcactt ctaaaatcag
     4681 taatcttttc cccattattt tatatttagt cgcagcatta gtaaccttaa caacaatgac
     4741 taggttcgtc gaggaagaaa gaaccaacgc aggcatatta aaagcactag gctatagtga
     4801 ccgccaagtt atctttaaat ttattattta tggttttata gcaggaacat tagggactac
     4861 tttgggtatc attggagggc attatctttt acctcgtatt atttctgata tcatttccaa
     4921 agacctgact attccaaaca cacagtatca tcttttctta aattacagtt tactagcctt
     4981 tgttttttca ttattaagca ttgttcttcc agtttttgtt atcactcgac gtgagttgaa
     5041 agaaaaagca gcatttttat tattaccaaa accaccagct aaaggatcta aaatagcttt
     5101 agagtatatc aattggattt ggaaaaaact gtccttcaca caaaaagtaa ctgctagaaa
     5161 tatttttcgt tacaaacaaa ggatgattat gaccatattt ggagttgctg gatcagtagc
     5221 gctcttattt tcaggtctag gaatacaatc ctccttaaag caaaccgtca atgaacattt
     5281 cggtcgtatt atgccctatg atatattgct aacatataac acaaatgctt ctcccccaaa
     5341 aatacttgaa ctactttcaa aagattcaaa aatagacaaa taccaaccaa tacaccttga
     5401 aaatcttgac gaatccattc ctggacaaat taataaacag tcaatttccc tattcataac
     5461 agacaaaaaa caattactac cttttattta tttacaagaa gcaacaacta ataagtcatt
     5521 gcacttgaat aataaaggga ttattatttc taaaaaatta gctcaatttt atcatgttaa
     5581 taccggtgat tttatccatt tatctcattc acaaacactt ccttctagaa aattaaaaat
     5641 aacaggagtt gtcaatgcga atgttggtca ctatattttt atgacaaaac aatactatcg
     5701 aactattttt aagaaagaag ctaaagataa cgcttttttg gttaagttaa ccaaacataa
     5761 aatcgcaaat aacttagcag aaaaactttt agaaattaat ggagttgagt ctcttacgca
     5821 aaatgctctt cagctggcta gtgtagaagc cgttgtgcgt tctttagatg gctctatgac
     5881 tattttagtt gtcgtatctt tattgttagc cattgtaatt ctctataatc tcactaatat
     5941 taatttagca gaacgtaagc gggagctatc aactataaag gtattaggtt tttataatga
     6001 agaagtaact ttatacattt accgcgaaac cattatttta tcaaccatcg gtgtgattct
     6061 aggcaccatt agcggtactt atttacaccg tcaaatgatg ttgctaattg gttcagatca
     6121 aatacttttt ggtgaaaaag tatcaccaac tacatttata ataccaataa gcgtagtagt
     6181 catcattcta ataagtctag gttttatagt taaccatcaa ttaaaaaaac tcaatatgtt
     6241 agatgccttg aaatcagtag attagtttat aaaaaagatt atcttttcta atcttt
//
LOCUS       DATRQA010000106         6221 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.106, whole genome shotgun sequence.
ACCESSION   DATRQA010000106 DATRQA010000000
VERSION     DATRQA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.106"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            102..1247
                     /locus_tag="VBD27_001565"
     CDS             102..1247
                     /locus_tag="VBD27_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7025566.1"
                     /translation="MIYFDNSATTIPYPEVLRTYQEVASKIYGNPSSLHELGTTSSRI
                     LEASRKQIASLLELKANEIFFTSGGTEADNWVIKGLAFEKQHFGNHIIVSDIEHPAVK
                     ESAKWLGEYGFEIDYAPVDDKGFVDVEALVKLIKPETILISIMAINNEIGSIQPIKAI
                     SDLLSDKPTISFHVDAVQAIGKIPTKDYLTERVDFASFSSHKFHGVRGVGFLYIKEGK
                     RISPLLTGGGQESDLRSTTENVAGIAATAKALRMVMDKEVVAIPKISKMKTIIHDELA
                     KYEDITLFSGKEGFSPNIITFGIKGVRGEVLVHAFEGHDIFISTTSACSSKAGKPAGT
                     LIAMGISTKLAQTAVRISLDDDNDMGQVEQFLTIFKQVYEKTKKVRG"
     gene            1249..2463
                     /gene="thiI"
                     /locus_tag="VBD27_001566"
     CDS             1249..2463
                     /gene="thiI"
                     /locus_tag="VBD27_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEO7025567.1"
                     /translation="MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVK
                     TDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSPSFKVEKNVDTLVKAVQEIMTSVYKD
                     GMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEIRDEAAY
                     ISYEDIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAHDLTRKLTKFGGNIQFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRIR
                     EDRNGLVIINGESLGQVASQTLESMQAINAVTATPIIRPVVTMDKLEIIDIAQKIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVTTIQPQAD
                     SDDVDDLIDDLL"
     gene            2565..3743
                     /locus_tag="VBD27_001567"
     CDS             2565..3743
                     /locus_tag="VBD27_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEO7025568.1"
                     /translation="MARIPYQKTLIFCVAVIIAIFILGLSKDLAQSKGQKVANNNTVK
                     TARVVANGDILLHDVLYASARQPDGTYNFTPYFKEVKSWIESADLAIGDYEGTISSEY
                     PLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRLGLDTIGV
                     YLKDRNKEDILIKRVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIK
                     KAEKEADITIVMPQMGIEYQKKPTTEQVILYHSMIKWGADIIFGGHPHVVEPSEVIKK
                     DGQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDVTIEKKGQKTVIKKVKAHPTLVE
                     AKANGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMNELVNLKW
                     "
     misc_feature    3819..3898
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            3933..4247
                     /gene="rplU"
                     /locus_tag="VBD27_001568"
     CDS             3933..4247
                     /gene="rplU"
                     /locus_tag="VBD27_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO7025569.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLDVEAGAEVTFNEVVLVG
                     GETTKVGTPVVEGATVVGTVEKQGKQKKVVSYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            4254..4592
                     /locus_tag="VBD27_001569"
     CDS             4254..4592
                     /locus_tag="VBD27_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEO7025570.1"
                     /translation="MIKATFTRNQSGYLYSAEISGHAGSGEYGFDVICAAVSTLSINF
                     INSLEALTTCQAQLIINDVEGGYMKIDLSSIPQHKEDKVQLLFESYLLGMTNLSKDSS
                     EFVSTVVMTH"
     gene            4614..4907
                     /gene="rpmA"
                     /locus_tag="VBD27_001570"
     CDS             4614..4907
                     /gene="rpmA"
                     /locus_tag="VBD27_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO7025571.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGANVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            5123..6028
                     /locus_tag="VBD27_001571"
     CDS             5123..6028
                     /locus_tag="VBD27_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7025572.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAAKLFVSQPSLSVAVRDLETELG
                     FQIFTRTTTGAVLTNQGMTFYENALEVVKSFDSFEKQFSQSEATEQEFSIASQHYDFL
                     PPLITAFSKCNDNFSYFRIFESTTIRILDEVAQGNSEIGIIYINSQNKKGLLQRLDKL
                     GLEFVELIPFKTHIYLGKDHPLASKTSLIMTDLEGLPTVRFTQDRDDYRYYSENFVEV
                     LDSSVTYNVTDRATLNGILERTQAYATGSGFLDSRSVNGITVIPLEDHLDNQMIYIKR
                     KDRNLSQMALKFVAVMEEYFKGFNS"
     gene            6037..>6221
                     /locus_tag="VBD27_001572"
     CDS             6037..>6221
                     /locus_tag="VBD27_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO7025573.1"
                     /translation="MRKIIIPIITILLIALDQLSKLWIVKHIELNQIKEFIPNIVSLT
                     YLRNYGAAFSILQNQQWL"
BASE COUNT         2102 a          923 c         1255 g         1941 t
ORIGIN      
        1 gagaattatc ataaatattg ttttgggatg ttcacaaact aatataaata gtaggaaaat
       61 tatgctataa taacactgat tgactttaga aggacagatt tatgatttat tttgataatt
      121 cggcaactac tattccctat ccagaagttt taaggactta tcaagaagta gctagtaaaa
      181 tctatggaaa cccctcaagt ttacatgagc taggaacaac atcttctcgg attttagagg
      241 ctagccgtaa acaaattgct agtttacttg agcttaaagc aaatgaaatt ttctttacct
      301 ctggtggtac agaggccgat aattgggtta ttaagggtct tgcgtttgag aaacaacatt
      361 ttggaaatca tattattgtt tctgatatcg aacacccagc tgttaaagag tctgctaaat
      421 ggcttggaga gtatgggttt gagattgatt atgcgccagt agatgataaa ggatttgtag
      481 acgtagaagc actggtcaaa ttaattaaac cagaaacgat tcttatttca attatggcaa
      541 taaacaatga aattggttct attcagccaa taaaagctat ttcagacttg ttgtcagata
      601 aacctactat atcgtttcat gttgatgctg ttcaagcaat aggtaagata ccgactaaag
      661 attatttgac tgagcgagtt gattttgcat ctttttctag tcataaattt cacggcgttc
      721 gtggggttgg ctttctatat attaaagaag gaaaacgtat ttcaccatta ctaacaggtg
      781 gtggacaaga atctgattta cgttcaacta ctgagaatgt cgcagggatt gcagctactg
      841 ctaaagcttt acgaatggtt atggacaagg aagtagtagc tattcctaaa ataagtaaaa
      901 tgaaaaccat tattcatgat gaattagcca aatatgagga tattacgtta ttttcgggta
      961 aggaaggttt ttcacctaac attatcacgt ttggtatcaa gggtgtccga ggagaagttc
     1021 ttgtccatgc ctttgaggga catgatattt ttatctcgac aactagcgct tgctcttcta
     1081 aagcaggtaa accagcagga actcttatag caatgggaat ttcaactaag ttagcccaaa
     1141 ctgctgtacg tatcagctta gatgatgata atgatatggg acaagtagag caatttttaa
     1201 ctatttttaa acaagtttat gaaaaaacta agaaagttag aggataagat gcagtattca
     1261 gaaattatga ttcgttatgg agaactctct actaagaaga aaaaccgtat gcgcttcatc
     1321 aataagttaa aaaataatat ggagcatgta ctctccattt atccagatgt ttcagtaaaa
     1381 acagatcgtg atagaggaca tgtatatctc aatggtacag attatcatga agttgcagag
     1441 tccttaaaag agatttttgg tatccaagct ttttctccat cttttaaagt agaaaaaaat
     1501 gttgatacat tggtaaaagc tgtccaggaa attatgactt ccgtttataa agatgggatg
     1561 acttttaaaa ttaccgcaaa acgtagtgac cactcatttg aattggatag ccgtgctcta
     1621 aatcatactt taggagatgc cgttttttca gtcttgccaa atattaaggc tcagatgaag
     1681 caaccagata tcaatcttaa agtcgagata cgagatgagg ctgcttatat ttcatatgag
     1741 gatattaggg gtgcaggagg attaccggta ggaacatctg ggaaagggat gctgatgttg
     1801 tctggtggga ttgattctcc ggtagcaggt tacctagcgt taaaacgtgg tgtagatata
     1861 gaagcagtcc attttgcaag tcctccttat actagcccag gtgcattgaa aaaagcacat
     1921 gatttaacac gtaaattgac aaaatttggt ggtaatattc aatttattga agttccattc
     1981 acagaaattc aagaggaaat taaggaaaaa gctcctgaag cctacttgat gacgttaaca
     2041 cgtaggttta tgatgcgtat tacagatcgt attcgtgagg accgaaatgg tcttgttatt
     2101 attaacggtg aaagtttagg gcaggtggca agccaaacgc tagaaagtat gcaagctatt
     2161 aatgctgtca ctgcaacacc gattattcgt cctgtggtca cgatggataa gctagaaatt
     2221 attgatattg ctcaaaaaat agatactttt gatatttcaa ttcaaccatt tgaggattgc
     2281 tgtacaattt ttgcaccaga tcgcccaaaa actaacccta aaattaagaa tacagaacag
     2341 tatgagaaac gtatggatgt agaaggtctg gtagagaggg cagttgcagg gattatggta
     2401 actactattc aacctcaagc agatagtgat gatgttgatg acttgattga cgatttatta
     2461 taatgaattt ttaaactaca gatttactct gtagtttttt attaaaatga ttgtgctgtc
     2521 ataaggttat aaaaaatgct ataatacgaa taaaggaggc aagtatggca agaatccctt
     2581 atcaaaaaac acttattttt tgtgtagccg ttattattgc tatttttatt ttggggttat
     2641 ctaaggattt agcacaatct aaagggcaaa aggttgcaaa taataatact gtaaaaacag
     2701 cacgtgtggt tgcaaacgga gatattttgc tgcacgatgt actatatgcc agtgcgcgcc
     2761 aacccgacgg cacttataat tttactcctt attttaaaga ggtaaaatct tggattgaga
     2821 gcgctgattt agcgattggt gattatgaag ggacgattag ttcagaatat cctttagcgg
     2881 gctatccttt atttaatgct cctaatgaga ttgcaactac tatgaaagaa actggatatg
     2941 atgtcgttga cttagcacat aatcatattc tggattcaca actggcaggt gctatcaata
     3001 ctgtcaagac ttttaatcgt ctgggattgg atactattgg ggtttattta aaggatagaa
     3061 ataaagaaga tatcttaatt aagcgtgtaa atggtattaa aattgctatt ttaggttatt
     3121 cgtatggcta caatggtatg gaagctaatg tttcaaagag tgactatgaa aaacatatgt
     3181 ctgatttaga tactaaaaag attaagcaag atattaaaaa ggctgaaaaa gaagcagata
     3241 tcaccattgt catgccacag atggggatag aatatcaaaa aaaacctact acagaacaag
     3301 taatacttta tcatagtatg attaaatggg gggcagatat tatattcgga ggacaccctc
     3361 acgttgttga accgtctgaa gtgataaaaa aagatggcca gaaaaaattt attatttact
     3421 ctatgggaaa ttttatttca aatcagcgtt tagaaacggt tgatgatatc tggacagaaa
     3481 gaggattatt aatggatgta accatagaga aaaaaggtca aaaaacagta attaaaaaag
     3541 ttaaggcgca tccaacacta gtagaagcaa aagcaaatgg ccgttatgtt gaagaaggct
     3601 ttccagctta tgattatagg acgctagttt tagaggatta tattgaaggt ggtaagaaac
     3661 gttctacaat tgatcagact atgaaagata aagttgataa agcatataag gaaatgaatg
     3721 agttagttaa tttgaagtgg taaagttgat aattttattt tgttttatct tgcataaaaa
     3781 aagagtttat gataaaattt gaatgttgac taaatgcaca tcctgtgcaa ccgcgcgaaa
     3841 atcgttagat taagtagggc aaggctctca cgatttatag gcgagtcttc acagagggaa
     3901 acctcacaag aaatttttat aggaggtgca tcatgagcac atacgcaatc atcaaaactg
     3961 gtggtaaaca agttaaagtt gaagtaggac aagctatcta cgttgaaaaa cttgacgttg
     4021 aagcaggagc agaagttaca tttaacgaag ttgttcttgt tggtggtgaa acaactaaag
     4081 ttggtactcc agttgttgaa ggagctactg ttgttggtac tgttgaaaaa caaggcaaac
     4141 aaaagaaagt tgtttcttac aaatacaaac ctaaaaaagg tagccaccgt aaacaaggtc
     4201 accgtcaacc ttatacaaaa gttgttatca acgcaatcaa cgcttaattt ttcatgatta
     4261 aagcaacttt tactcgtaat caatcaggct atctttatag tgctgaaatc agcggtcatg
     4321 ctggtagtgg cgagtacggc tttgatgtga tttgtgcagc tgttagtact ttatcaatta
     4381 attttattaa ttcattagag gctttaacaa cctgccaagc gcaattgatt atcaatgacg
     4441 ttgaaggtgg ttatatgaag atagatttat cttctatacc acaacataaa gaggataagg
     4501 ttcaattatt atttgagtct taccttcttg gaatgactaa tctgtctaaa gattcttctg
     4561 aatttgttag tacagtagtc atgacacact aatttaagag aggacacaac attatgttaa
     4621 aaatgaatct tgctaacttg caacttttcg cccacaaaaa aggtggaggt tctacatcaa
     4681 acggacgtga ttcacaagca aaacgtcttg gagctaaagc agctgacgga caaacagtat
     4741 caggtggatc aattctttac cgtcaacgcg gtacacacat ctatccaggt gctaacgttg
     4801 gacgtggtgg ggacgataca ctttttgcta aagttgaagg tgttgttcgt ttcgaacgta
     4861 aaggacgcga taaaaaacaa gtgtctgttt acccaatcgc aaaataatgc ctagttaaga
     4921 ctttatgact attcataagg tcttttatta ttgtcagagc tttcattttg taacaaaaga
     4981 caatatgtta agagatttat cattaatact cataaacttt gttgttcttt tatagtgaaa
     5041 tagtcctaat tcatgaagaa ttttccgcaa ggatagttat tttttggtat aatgaagggt
     5101 actaaaatga ggaagtgata aaatgaatat tcaacaactg cgttatgttg ttgctatcgc
     5161 aaatagtggt acttttcgtg aagcagctgc aaaactattt gttagtcagc caagtttatc
     5221 tgttgccgtt cgtgatttgg aaacagaatt agggtttcag atttttacgc gtacaactac
     5281 tggtgcagtg ttgaccaatc aaggcatgac tttttatgag aatgctttag aagttgtgaa
     5341 aagctttgat tcattcgaaa aacagttttc gcaatctgaa gctacagagc aagaattctc
     5401 aatagcaagt caacattatg attttctacc tcctttaatc acagcgtttt caaaatgtaa
     5461 tgataatttt agctatttcc gtattttcga atcaacaaca atacgtatct tagatgaagt
     5521 tgctcaaggg aatagcgaaa taggtatcat ttatatcaat agtcaaaata aaaaaggtct
     5581 acttcagcgg ttggacaaat tggggttaga gtttgtagag cttattcctt ttaaaactca
     5641 tatttatctt gggaaagacc atcccttggc tagtaaaaca tctctgataa tgacagattt
     5701 agaaggatta ccaaccgtac gttttacaca ggatagagat gattatcggt attactcgga
     5761 gaattttgtg gaagtacttg atagttctgt tacctataat gttactgata gagcaacatt
     5821 aaatggtatt ttagaaagaa cccaagctta tgcgacgggc tcaggattct tggatagccg
     5881 cagtgtgaat ggtatcactg ttattccatt agaagatcat ctagataacc aaatgattta
     5941 tatcaaacga aaagatcgta atttatctca gatggcttta aaatttgttg ctgttatgga
     6001 agagtatttt aagggattta attcatagga gaagtaatga gaaagattat tataccaata
     6061 ataactattt tgttgattgc tttagatcaa ctatctaagt tatggattgt taaacatatt
     6121 gaactaaacc aaattaagga attcattcca aatatcgtta gtctaactta tctgagaaat
     6181 tatggtgctg cattttctat tttgcaaaat caacaatggc t
//
LOCUS       DATRQA010000107         6174 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.107, whole genome shotgun sequence.
ACCESSION   DATRQA010000107 DATRQA010000000
VERSION     DATRQA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.107"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            154..441
                     /locus_tag="VBD27_001573"
     CDS             154..441
                     /locus_tag="VBD27_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO7025574.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILSDNGATVVESKDWE
                     KRRLAYEIQDFTEGLYHIVNVEAEDAVALNEFDRLSKINGDILRHMIVKVD"
     gene            453..944
                     /locus_tag="VBD27_001574"
     CDS             453..944
                     /locus_tag="VBD27_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7025575.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGER
                     EADFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQQGQRVYVTEVVAESFQL
                     LESRATREGGSPNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
                     LPF"
     gene            989..1228
                     /locus_tag="VBD27_001575"
     CDS             989..1228
                     /locus_tag="VBD27_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO7025576.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            1398..2342
                     /locus_tag="VBD27_001576"
     CDS             1398..2342
                     /locus_tag="VBD27_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7025577.1"
                     /translation="MKQMFLSTAIEFKEIETFEPGAWINLVNPSQEESEQVADQFGID
                     IDDLRAPLDVEETSRISVEDDYTLVIVDVPTYEERNNKSYYMTIPMGIIVTDNAVITT
                     CLEHLTLFDHFYRRRVKNFYTFMKTRFVFQLLYRNAELYLQALRTIDRQSDKIEAQLE
                     SATRNEQLIDMMELEKSIVYLKASLKFNERIVKKLTSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMANIYENVLNAMTETTASIIGNNQNTIMKTLALVTMTLDIPTVIFSAYGMNF
                     KNNWMPLNGLAHGFIYVVLLAFLMSSFVVFYFIRKKWF"
     gene            2367..3038
                     /locus_tag="VBD27_001577"
     CDS             2367..3038
                     /locus_tag="VBD27_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HEO7025578.1"
                     /translation="MTLQDLTKKNQEFVHIATNQLLADGKSDAEIKAILEEHLPEIID
                     NQKKGITARSLLGAPTTWAASFTERPEDKARVSVQKNTNPWLMWLDTSLLFLGLVTAL
                     NGLMLLFGQSNVNTGLISILTLGFGGGAAMYVTYYYIYRHMGKPKSERPGWLKSFAVL
                     ALVMLVWFALFAVVPLLPATINPKLPEVVLFIIALASFGLRFYLQRKYNIQSSMAPVA
                     PQQRR"
     gene            3170..5998
                     /gene="uvrA"
                     /locus_tag="VBD27_001578"
     CDS             3170..5998
                     /gene="uvrA"
                     /locus_tag="VBD27_001578"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO7025579.1"
                     /translation="MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLALPERTKMQILAPIIRRKK
                     GQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELSKSKMHNIDIVVDRLINKEGIRSRL
                     FDSVEAALRLSDGYVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDIDLVIPDRSKTLREGALVPWNPISSNYYPTMLEQAMTQFGVDVDTP
                     FEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYHETNSDYT
                     RNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQVSDLSIADHLELLETLRL
                     SSNEQLIARPIIKEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYVLDEPSIGLHQRDNDRLIDSLKKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGA
                     GAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEIKGAAENNL
                     QNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     ESCSGDGIIKIEMHFLPDVYVPCEVCHGTRYNSETLEVHYKEKNIAQILDMTVNDAVT
                     FFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQ
                     IVAIGTPEEVAENPKSYTGYYLKEKLAR"
BASE COUNT         1985 a         1021 c         1230 g         1938 t
ORIGIN      
        1 aaataaaaaa tgtctttaaa tgatatcagg gaaataaaaa cataaaaacc ttgcattatt
       61 aacttatttc tactataata atatcttgtg agcataacct cacttactca tgaaaaattc
      121 atgagtcatt agaccaaaag gaggaacata tcaatggcta aatacgaaat tctttatatt
      181 attcgtccaa acattgaaga agaagctaaa aacgctttgg tagcacgttt tgattctatc
      241 ttatctgaca acggtgcaac tgttgttgaa tcaaaagatt gggaaaaacg tcgtcttgca
      301 tacgaaatcc aagatttcac tgaaggactt taccatatcg ttaacgttga agctgaagat
      361 gcagtagctc ttaatgagtt tgatcgtctt tcaaaaatca acggtgacat tcttcgtcac
      421 atgatcgtta aagtagacta agaaggtagt ttatgattaa taatgtagta cttgtaggtc
      481 gcatgacccg tgatgcagaa cttcgttata caccaagtaa tcaagcggta gccacttttt
      541 cacttgcagt taatcgtaat tttaaaaatc aatctggcga acgtgaggct gattttatta
      601 actgtgttat ttggcgccaa caagctgaaa acttggctaa ctgggcaaaa aaaggtgctt
      661 tggttggaat tacaggtcgt atccaaacgc gtaattatga aaaccaacaa ggtcaacgtg
      721 tctatgtaac agaagttgtt gcggaaagtt tccaattatt ggaaagtcgt gctacacgag
      781 aaggtggttc acctaactct tataataacg gtggctataa caatgctccg tcaaataata
      841 gttattcagc ttcttctcaa caaacaccta attttagtcg tgatgagagt ccgtttggta
      901 actcaaatcc aatggatatt tcagatgatg atctaccatt ctaatataat agatggacag
      961 aatttaaaac tataaaggag aaataaacat ggctcaacaa cgtcgtggcg gattcaaacg
     1021 ccgtaaaaaa gttgattata tcgcagctaa caagattgaa tatgttgatt acaaagatac
     1081 agagcttctt agccgtttcg tttcagaacg tggaaaaatt ttaccacgtc gtgtaacagg
     1141 aacttcagct aaaaaccaac gtaaagtaac aacagcaatc aaacgtgctc gtgttatggc
     1201 tcttatgcct tacgtaaatg aagattaata taaaatagcc ctagcggcta tttttttatt
     1261 ctttttacca ttattattgg tatttacgag tcaaataata gaaaaattta ggataccacc
     1321 gtattaaccg tgcaaaacaa atggttttcc tgtataatac taaggtatgg attttttata
     1381 acaaaggagg aatcgccatg aagcaaatgt ttctttcaac ggcaattgaa tttaaagaaa
     1441 ttgaaacatt cgagccaggt gcttggataa atctggttaa tccctcccag gaagaatccg
     1501 aacaagtagc tgatcaattt ggtattgata ttgatgattt acgtgctccc ctcgatgtgg
     1561 aagaaacttc ccgtatctcg gttgaagatg attatacttt agttattgtt gacgttccta
     1621 cctatgaaga acgcaataac aaaagttatt atatgactat tcctatgggc attatcgtta
     1681 ctgacaacgc tgttattact acttgcctag aacatttgac gctgtttgat cacttttata
     1741 gacgacgtgt caaaaatttt tataccttta tgaagacacg ttttgttttc caattgcttt
     1801 atcgcaatgc cgaattatac ttacaggctt tgcgtactat cgataggcag agcgataaaa
     1861 ttgaagcaca actagagagt gcaactcgaa atgagcaatt gatcgatatg atggaattgg
     1921 aaaaatctat tgtctatcta aaggcctctt taaaatttaa tgagcgaatc gtcaaaaaat
     1981 tgaccagtag taccagtagt ttaaaaaaat acattgaaga tgaagatctg cttgaagata
     2041 cattgatcga aactcagcag gctattgaaa tggcaaatat ctatgaaaat gtcttaaatg
     2101 ccatgacaga aacgactgct tcaatcatcg gtaataatca gaataccatt atgaaaacct
     2161 tagccctagt aactatgaca ttggatattc caacggttat tttttcggcg tatgggatga
     2221 actttaaaaa taattggatg cctttgaatg gtctcgcaca tggctttatc tatgttgttc
     2281 ttttggcctt tttgatgagt tcatttgttg tattttattt tattcgtaaa aaatggtttt
     2341 aatagtatat taatcaagga gttcacatga ctttacaaga cttaactaaa aagaatcaag
     2401 agtttgttca tattgcaact aatcaacttt tagctgatgg aaaatcagat gcagaaatca
     2461 aagctatttt agaagaacat ctcccagaga ttatagataa tcagaaaaaa ggaattaccg
     2521 cacgctcact cttaggagca ccaactactt gggcagcctc attcactgag cgacctgagg
     2581 ataaagctag agtatctgta caaaaaaata ccaatccttg gttgatgtgg ttagatactt
     2641 ccttactatt tcttggctta gtaactgctt tgaatggttt aatgttgcta tttggtcaat
     2701 ctaatgtcaa tacagggctt atttcaattt taactcttgg ttttggtggt ggtgcggcca
     2761 tgtacgttac ttattactat atttatcgcc atatgggtaa accaaaatct gaaagaccag
     2821 gctggcttaa atctttcgca gtactggcac tagttatgtt agtttggttt gccttatttg
     2881 cagtagtacc tcttctacct gcaactatta atcctaaact tcctgaggtt gttttgttta
     2941 ttattgcttt agcatcgttt ggtttgcgct tttatttaca acgtaaatat aatattcaaa
     3001 gctcaatggc accagtggca ccacaacaaa gaagataata acaaagagta tgaacgtatt
     3061 agttcatctc ttttttctat gaaatttaaa actaaaaaaa tactgacttt attaaaattt
     3121 gatataataa gtcaacacta ttcaaaaagg agtgctgagg cacaacatta tgcaagataa
     3181 attaatgata agaggagcaa gagctcacaa tttaaaaaat ataagtgtcg acattccacg
     3241 agacaagtta gttgtcgtta cgggactatc aggatcaggt aagtctagtt tggcctttga
     3301 cactatctat gcagaaggac aacgtcgtta cgttgaaagt ctttctgctt acgcgcgcca
     3361 atttttggga aatatggaaa agcctgatgt agattctatt gatggcctta gtccagctat
     3421 ttctattgat cagaaaacaa caagtaagaa tcctcgctct actgttggga ctgtaacaga
     3481 aattaacgat tatttacgtt tgctttatgc tcgtgttgga accccatatt gtatcaatgg
     3541 tcacggagcc atcacagcat cttctgtaga gcaaattgtg gataaggtac tagcgcttcc
     3601 agagcgaaca aagatgcaaa ttttagcccc aattatacgt cgaaaaaaag gacaacacaa
     3661 atcaacattt gagaaaatac aaaaagatgg ttacgttcgt gttcgtattg atggtgatat
     3721 tcacgatgtt actgaggtgc ctgaattatc aaaaagtaag atgcacaata ttgatattgt
     3781 agtagatcga ttaattaata aagagggcat tcgttcccgc ttatttgatt ccgttgaagc
     3841 ggctctacgt ttgtcggatg gttatgtcgt tattgataca atggatggca atgagttatt
     3901 gttttcagag cattattctt gtcctgaatg tggttttact gttcctgaat tagagccacg
     3961 ccttttttct tttaatgctc cctttggttc ttgtccgacc tgtgatggct taggaattaa
     4021 gttagaagta gatatagatt tggttattcc agatagaagt aagaccctta gagaaggggc
     4081 cttagttcct tggaatccaa tttcatcaaa ttattatcca acgatgctag agcaagctat
     4141 gactcaattt ggtgttgatg tggatacgcc atttgaaaaa ttaagcaagg cggagcagga
     4201 cctagcccta tatggttcag gcgaacgaga gttccatttc cattatatta acgattttgg
     4261 aggcgagaga aatattgatt tgccttttga aggggttgtc aataatatta atcgtcgtta
     4321 ccatgaaact aatagcgatt acacacgcaa tgttatgcga gaatatatga acgagctaaa
     4381 atgtaacact tgtcatggct atcgtctcaa tgatcaagca ctttgcgtaa gagttggggg
     4441 agaagaaggt cttaatattg ggcaagtctc agatttatcg atagcagacc atttagaact
     4501 tttagaaaca ttgcgtttat cttcaaatga acaattaatt gctcgaccaa ttatcaaaga
     4561 aatccatgac cgtctaagtt tcttaaataa tgttggactt aattatttaa atctgtcacg
     4621 ttctgcagga acactttccg ggggcgagag tcaacgtatt cgtttggcta ctcaaattgg
     4681 gtctaattta tcaggtgtcc tttatgtact tgatgagccg tccattggac ttcatcagcg
     4741 agacaatgac cgtttgattg atagtcttaa gaaaatgcgt gatttgggta atacgcttat
     4801 tgttgttgaa catgatgaag atacaatgat ggcagctgat tggttaatcg atgtgggacc
     4861 tggcgctggt gcgtttggtg gtgagattgt ggcctctggt acaccgaagc aagtagctaa
     4921 aaatactaaa tctattacag gacaatattt gtcaggtaag aaagtgattc ctgtgccatc
     4981 tgaacgaaga gtaggtaatg gtcgcttttt agaaatcaaa ggtgcggcag aaaacaacct
     5041 acaaaattta gatgtcaagt tccctctcgg taaatttatt gccgtaactg gtgtctcagg
     5101 ttctggaaaa tcaaccttaa ttaatagtat tttgaaaaaa gctgttgcgc agaagcttaa
     5161 ccgtaactca gataaaccag gtaaatatgt ttctttagaa ggcattgaat atgttgatcg
     5221 attgattgat attgatcaaa gtccaattgg tcggactcca cgttctaatc ccgcaaccta
     5281 tacaggtgtt tttgatgata ttcgtgatct ttttgctcaa actaatgagg caaagattcg
     5341 aggttataaa aaaggacgct tctcatttaa cgtcaaaggt ggacgttgtg agtcttgttc
     5401 tggcgatggt atcatcaaaa ttgagatgca ttttttacca gatgtctacg ttccttgtga
     5461 agtatgccac ggcacgcgct ataatagtga gactctggaa gtacattaca aagaaaaaaa
     5521 tattgcacaa attcttgata tgacagtgaa tgatgcagtt acattttttg cagctattcc
     5581 taaaattgct cgtaagttac agactattaa ggatgttggt ttgggatatg tcactttggg
     5641 gcaaccagct acaaccttat caggaggaga agctcagcga atgaagctgg caagtgaatt
     5701 acataagcgt tcgacaggta agagtcttta catattagac gaaccaacaa cggggcttca
     5761 tgcagatgat attgctcgtt tacttaaggt tttggatcgt tttgtagatg atggtaatac
     5821 agtgcttgtt attgagcata accttgatgt tattaaaact gctgaccaca ttattgatct
     5881 tggccctgaa ggaggtattg gtggcggaca aattgtagct atcggaacac cagaagaagt
     5941 tgctgaaaat cctaaatcct atacaggata ttatttaaaa gagaagttag caagatagtt
     6001 tattgggagc tgagtaatat tactcagttc ctttttgtaa gctaacattg cgtagaagtc
     6061 attaaatgga gaacgcttta ttaggaaaaa tagtggggag atattttaaa aaaaggaaat
     6121 aatgatgttt tgaaaaaaat agtttctatg agaatctttt tcttgacaat aaaa
//
LOCUS       DATRQA010000108         6167 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.108, whole genome shotgun sequence.
ACCESSION   DATRQA010000108 DATRQA010000000
VERSION     DATRQA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.108"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            12..872
                     /locus_tag="VBD27_001579"
     CDS             12..872
                     /locus_tag="VBD27_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7025580.1"
                     /translation="MPYPYKANHSIESITLKVNDLENLVNFYSDIIGLTVIDKSSTRA
                     LLGVNQKIPLIILEKTELEKHSTYGLYHTAILVPDEYHLSLALNHLLSQHIPLEGGAD
                     HGYSNAIYLSDPEGNGIEIYNDKDISMWDIRESGQIIGITERLDIDNLLDSLVNVPNN
                     YKLSEKTRIGHIHLSVKDAKISSKLYQNVFGLDEKFAIPTASWIASGNYHHHLAFNNW
                     AGPNLSKNQENRPGISLLTIAYNDDNLFRDSLKKAQLYQLTFLEKQDHYYIIEDFDGI
                     RIKVVLKPEN"
     gene            915..1646
                     /gene="yaaA"
                     /locus_tag="VBD27_001580"
     CDS             915..1646
                     /gene="yaaA"
                     /locus_tag="VBD27_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEO7025581.1"
                     /translation="MIKILIPTAKEMKVCQNIARPKLSAQTQIIIDYFSTLTVSDLED
                     IYRINTSAARCEAQRWQDFKCKQLTLNPAIKLFNGLMYRNIKRHNLSTSEAQFMENSV
                     FITSALYGIIPAMTLISPHRLDFNTKIKINNNSLKVFWRENYDTFMQSDDIMVSLLSN
                     EFKTVFSPKERQKLIHLNFIEDRDGQLKTHSTISKKARGKCLTSMMENNCQTLEHLKQ
                     LRFDGFCYDNELSDSKQLTFVKKQT"
     gene            complement(2003..2620)
                     /gene="nrdG"
                     /locus_tag="VBD27_001581"
     CDS             complement(2003..2620)
                     /gene="nrdG"
                     /locus_tag="VBD27_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEO7025582.1"
                     /translation="MSEEASWNTPKPGEWKSEELSHGHIIDYKAFNFVDGEGVRNSLY
                     VAGCMFHCKGCYNTATWSFRAGIPYTKELEDQIMKDLAQPYVQGLTLLGGEPFLNTGI
                     LLPLLQRIRRELPEKDIWSWTGYTWEEMMLETQDKLEMLSLIDILVDGRFDQSKRNLM
                     LQFRGSSNQRIIDVQKSLKEGEVVIWEGLNDGDNSYEQVKRDYLL"
     gene            complement(2693..3184)
                     /locus_tag="VBD27_001582"
     CDS             complement(2693..3184)
                     /locus_tag="VBD27_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7025583.1"
                     /translation="MKLRRPVLEDKEEILAMYKEFQKESSSMDGGFYEPTTHFEDWLD
                     HNLNMELGIGVPDNFVPYIQFVSFDNDNNAIGFLNLRLRLNDTLLEKGGHIGYSIRPR
                     QRGKGYAKEQLKLGIEQAHLKNINEILVTCHVDNDASKSVILANGGILEDCRHQTERY
                     WIT"
     gene            complement(3193..4125)
                     /locus_tag="VBD27_001583"
     CDS             complement(3193..4125)
                     /locus_tag="VBD27_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7025584.1"
                     /translation="MLQLGIVGLGGISQKAYLPYMRQVTGVHWHLFTRQKQILEEVNM
                     LFGSSTAYDSLDSLAEHPLDGVFIHVATSAHFDIAKLFLKKGIPVFMDKPLTEDYTST
                     KALYDLAKDHKTFLMAGFNRRFAPRIMEMKKVEDKNHIRTFKNAVNAPADFQYKLFDM
                     FIHPLDTALFLTNNVVKRGYFVTKRDGNKILQVSVTLETDSEIIEASMNLQSGSRREI
                     IEIESPEVTYSLDDLSNLSVIDGFDRRAIGFGSWASTLEKRGFEPMIDAFIQAITTGV
                     NPISPKSSLLSHFICDQINKANAPFGMLNLKIME"
     gene            complement(4138..4281)
                     /locus_tag="VBD27_001584"
     CDS             complement(4138..4281)
                     /locus_tag="VBD27_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025585.1"
                     /translation="MGKYQLDYKGQAQVQKFHEKHSTGENANQKSRLKDLRKQFLEKA
                     KKK"
     gene            complement(4356..>6167)
                     /gene="nrdD"
                     /locus_tag="VBD27_001585"
     CDS             complement(4356..>6167)
                     /gene="nrdD"
                     /locus_tag="VBD27_001585"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO7025586.1"
                     /translation="VNENANKDSDVFNTQRDLTAGIVGKSIGLKMLPSHVANAHQKGD
                     IHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQTATAQISQIIANVA
                     SSQYGGCTADRIDEFLAPYAQLNYQKHLKDAKEWVIEDKQEDYARAKTQKDIYDAMQS
                     LEYEINTLFTSNGQTPFTSLGFGLGTNWFEREIQKAILKIRIQGLGSEHRTAIFPKLI
                     FTLKKGLNLEENSPNYDIKQLALECATKRMYPDVLSYDKIIDLTGSFKAPMGCRSFLQ
                     GWRDANGQDVTSGRMNLGVVTVNLPRVAMESNGDMDKFWEIFNERMSIARDALVYRVE
                     RVKEAIPANAPILYQYGAFGERLGKYENVDRLFNHRRATVSLGYIGLYEVASVFYGGD
                     WEDNHQAKAFTVDIVRKMKQLCADWSDEYDYHFSVYSTPSESLTDRFCRLDTEKFGIV
                     TDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPETGASGGFIHYCEYPVLQQNPKAL
                     EAVWDYAYDRVGYLGTNTPIDKCYQCQFEGDFTPTDRGFTCPNCGNSDPKTVDVVKRT
                     CGYLGNPQARPMVNGRHKEISARVKHMNGSSIKNQGN"
BASE COUNT         1998 a         1242 c          917 g         2010 t
ORIGIN      
        1 gaggattaac tatgccgtat ccatacaaag ccaatcattc cattgaatca atcaccttaa
       61 aagtaaatga cctcgaaaac ctcgtaaact tctatagcga tatcattggg ttaactgtta
      121 ttgacaaatc aagcactaga gccttactag gggtaaatca aaaaatccct cttattatcc
      181 ttgaaaaaac tgaacttgaa aagcatagta cctatggcct ttatcacact gctattttag
      241 taccagatga ataccatctc agtctagctc taaatcattt attatctcag catatccctc
      301 tagaaggtgg agctgaccat ggctatagca atgctatcta tctaagtgat cctgaaggta
      361 atggaattga aatttataat gataaggata tctctatgtg ggatatcaga gagagtggac
      421 aaattattgg tattactgaa agactagata ttgacaattt acttgacagc cttgtcaacg
      481 ttcctaataa ctataagctt tctgaaaaaa ctcgtattgg tcatattcat ttgagtgtta
      541 aagatgctaa gattagctca aaactctatc aaaatgtttt tggccttgat gagaaatttg
      601 caatcccgac agctagttgg attgcatccg gtaactacca tcatcatcta gcctttaata
      661 attgggctgg tccaaatctt tctaaaaacc aagaaaatag acccggaatt tccttattga
      721 caattgctta caatgatgac aaccttttta gagatagctt aaaaaaagcg caactttacc
      781 aactgacttt ccttgaaaaa caagatcatt attatattat tgaagatttt gatggcatcc
      841 gaattaaagt tgttctaaaa ccagagaatt aaccatcact tgacttttga ctcttcattt
      901 tacataatgt caatatgata aaaattttga tcccaactgc aaaagaaatg aaagtgtgtc
      961 aaaacattgc aaggcctaaa ctgagtgcac aaacgcaaat tataattgat tatttttcta
     1021 ctttaactgt ttcagactta gaggatatct acagaattaa tacaagtgca gctcgttgtg
     1081 aggctcaaag atggcaagat tttaaatgta aacaacttac tctgaatcct gctattaagt
     1141 tattcaacgg tcttatgtac cgcaatatca aaaggcacaa cttatccaca agtgaagcac
     1201 aatttatgga aaattctgtt tttattacta gtgctcttta tggcatcatt cctgctatga
     1261 cactaatatc ccctcatcgt ttagatttta acactaaaat caaaatcaac aataactctc
     1321 ttaaagtttt ttggcgtgaa aattacgata cttttatgca gtcagatgac attatggttt
     1381 ctttgctttc taatgaattt aaaaccgtct tttctcctaa agagcgtcaa aaactaatac
     1441 atcttaactt tattgaagat cgagatggcc aattaaaaac ccactctacc atatcaaaaa
     1501 aagcaagagg aaagtgttta acttctatga tggaaaataa ttgccaaact ctagaacact
     1561 taaagcaatt acgctttgat ggcttctgtt atgataatga actctcagac tctaagcaat
     1621 taacttttgt taaaaaacag acttaagtaa ctatttatcc acaatttatt cacacaaaaa
     1681 gcaagagata atcgtatctc ttgctttttg acaaacttgt aaggtctaaa aacctctcta
     1741 tatcaaggtt tcaaataata ttaacaaaaa ataattgtta tcaacaactt gtggataact
     1801 atggggatag acagaggata atctgtttat ataaagttga ttacttaaca gtaatgtcat
     1861 tgtatatacc tttagatttc aaaaatactt gttaaattaa caataataaa cgtcaacatc
     1921 ttgtcacaga ctgtcaaata cttataaaca agctgtggat aaacataact ttcaatttct
     1981 atttaaaagt taatttttga cactaaagta aataatctcg tttgacttgt tcataactat
     2041 tgtcaccatc gtttaatcct tcccaaatca cgacttcacc ttcttttaaa gatttttgta
     2101 catcaatgat tctttgattt gaagatcctc taaattgtag cattaaattt cgcttacttt
     2161 gatcaaacct accatccact aaaatatcaa tcaaagataa catctccaat ttatcttgag
     2221 tctccagcat catttcttcc caagtatacc ccgtccatga ccaaatatct ttttctggca
     2281 attcacgtcg gatacgctga agaagtggca gcaaaatacc tgtatttaag aacggctctc
     2341 cacctaataa cgttaaacct tggacgtaag gttgtgccaa atctttcata atttgatctt
     2401 caagctcttt tgtataaggg attccggctc gaaaggacca tgtagcagta ttataacacc
     2461 ctttacagtg gaacatacat ccagctacgt agagtgaatt tctaacacct tcaccatcta
     2521 caaaattaaa agctttataa tcaatgatat ggccatgact taattcttcg cttttccact
     2581 ccccaggctt tggagtattc caacttgctt cttctgacat ttctaacctc tctattgata
     2641 tgaaaagaac cagaactaac tggtcttttt attattatct taaagtacta tcttaggtta
     2701 tccaataacg ctctgtctga tgacgacaat cttctaaaat accaccgtta gctaatatta
     2761 ctgacttgct ggcatcattg tctacatggc atgtcacaag tatttcattg atatttttta
     2821 gatgtgcttg ctcaattcct agcttcaact gttctttagc ataaccttta ccacgctggc
     2881 gaggtcgtat ggaataacca atatgtccac ctttttctaa aagagtatca tttagtctta
     2941 aacgtaaatt taagaaacct atagcattat tgtcattgtc aaaagacaca aactgaatat
     3001 atggaacaaa attatcagga acacctatcc ctaactccat atttaggtta tgatctagcc
     3061 agtcttcaaa atgcgtagtt ggttcataaa aaccaccatc cattgaacta ctttcttttt
     3121 gaaactcttt atacatggct aaaatttctt ctttatcttc taacactggg cgccgtaatt
     3181 tcatattgtt tcctattcca ttatcttcaa gttaagcatt ccaaaaggag cattcgcttt
     3241 attaatctgg tcacaaataa aatgactcaa taaactagac tttggtgata taggattgac
     3301 acctgtagtg atagcttgaa taaaagcatc aatcattggt tcaaaaccac gtttctctag
     3361 agtgcttgcc caagaaccaa aaccgatagc acgacggtca aagccatcaa taacagaaag
     3421 atttgacaaa tcatctaatg aataggttac ttctggcgac tctatttcaa ttatttctcg
     3481 acgagacccc gactgcaggt tcatgcttgc ttctattatc tcactgtctg tttctagcgt
     3541 tactgatact tgtaatatct tattcccatc acgtttagtc acaaagtatc ctcttttaac
     3601 aacattattt gtcaaaaata gcgccgtatc taaaggatgg ataaacatat caaaaagctt
     3661 atattgaaaa tccgctggtg cattgacagc atttttaaag gtgcgaatat ggtttttatc
     3721 ttctactttt ttcatttcca taatacgtgg tgcaaagcga cgattaaaac ctgccattaa
     3781 aaatgtttta tgatccttcg ccaaatcata caatgcttta gttgaagtat aatcttctgt
     3841 caaaggctta tccataaaaa ctggaatacc cttttttaaa aaaagctttg ctatatcaaa
     3901 gtgagcacta gtcgcgacat gaatgaagac tccatccaaa ggatgctcgg ctagactatc
     3961 taaactgtca taagctgtag aagaaccaaa aagcatatta acttcttcta aaatctgttt
     4021 ttggcgtgta aaaagatgcc agtgcacacc tgtcacttga cgcatataag gtagataagc
     4081 tttctgtgaa atacctccta aaccgacaat tccaagttgc aacatagcat cctccttcta
     4141 ttttttcttt gccttttcca aaaattgttt ccttaaatct tttagacgtg atttttgatt
     4201 agcattctct ccagtagagt gcttctcatg gaacttctgt acttgtgctt gccctttgta
     4261 atctaattgg tatttcccca tatcttaata actccttcta ttttatcttc ttattttaac
     4321 atataataga ccagaggttt taatagaggt tttaattaat taccttgatt tttaatactt
     4381 gatccgttca tatgttttac acgagcagag atttccttat gacgaccatt aaccatcgga
     4441 cgagcctgtg gattacctaa gtaaccacat gtacgtttga caacatctac tgtcttaggg
     4501 tcgctgttac cacaatttgg acaagtaaag cctcgatctg tcggtgtaaa atccccttca
     4561 aattgacatt ggtaacactt atcaattggt gtattagtac ctagataacc tacccgatca
     4621 taagcatagt cccaaactgc ttctaaagct tttggattct gttgcaaaac agggtattca
     4681 cagtagtgaa taaatcctcc tgatgcaccc gtttcaggat agattttctc aaaatctagt
     4741 ttttcaaaag gtgttggatt cttacgcaca tcataatgga atgaattagt gtagtattcc
     4801 ttatcagtaa tatcagttac tataccaaat ttttcagtat ctaaacgaca aaaccggtca
     4861 gtcaagctct ccgatggtgt cgaataaact gaaaaatgat aatcatactc atcagaccaa
     4921 tctgcacata actgcttcat cttacgaacg atgtctacag taaatgcttt agcttgatga
     4981 ttatcttccc agtcaccacc ataaaaaaca ctcgccacct catataaacc aatataacca
     5041 agagacaccg ttgcacggcg gtgattaaag agtcgatcaa cattctcata ttttcctaaa
     5101 cgttctccaa atgctccata ctggtaaaga atgggcgcat ttgctgggat agcctctttg
     5161 acacgttcca cacgataaac caaggcatct ctagcaatac tcatacgttc attaaatatc
     5221 tcccagaatt tatccatatc accgttagac tccatggcaa cacgcggtag attaactgtt
     5281 acaactccaa gattcatacg accagatgtt acatcctgac catttgcatc tcgccaccct
     5341 tgtaggaagg aacgacagcc cataggggct ttaaaagagc ccgttaagtc aataatttta
     5401 tcataagaaa ggacatctgg atacatccgt tttgtcgcac actctaatgc taattgttta
     5461 atatcataat tggggctatt ttcttctaaa ttaagacctt ttttaagcgt aaaaattaat
     5521 tttggaaaaa tagcagtacg atgctcgctt cctaaacctt gaatccgaat tttcaaaatc
     5581 gctttttgaa tttctcgttc aaaccaatta gttcccaatc caaaaccaag cgaagtaaat
     5641 ggcgtttgcc catttgaagt aaataaagta ttaatctcat actccaaaga ttgcatggca
     5701 tcatatatat ctttttgtgt tttagcgcgc gcatagtcct cttgtttatc ttcaataacc
     5761 cattcttttg catcttttaa atgtttttga taatttaact gtgcataagg agctaaaaac
     5821 tcatcaatgc gatctgctgt acagccacca tattgacttg aagcaacgtt cgcaataatc
     5881 tgtgaaatct gagcagtcgc tgtctgaata gattttggac tctcaacttc tgcattacca
     5941 atcttaaaac catttgcaag cattccctta aaatcgatta aacagcaatt tgtcatggga
     6001 gtgtatgggc tgtaatctaa atcatggtaa tggatatcac ccttttgatg tgcatttgct
     6061 acatgagaag gtaacatttt cagaccaatt gatttcccta caatacctgc tgttaaatca
     6121 cgttgggtat taaacacatc agagtcttta ttagcattct cattaac
//
LOCUS       DATRQA010000109         6146 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.109, whole genome shotgun sequence.
ACCESSION   DATRQA010000109 DATRQA010000000
VERSION     DATRQA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.109"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(258..2234)
                     /gene="ftsH"
                     /locus_tag="VBD27_001586"
     CDS             complement(258..2234)
                     /gene="ftsH"
                     /locus_tag="VBD27_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO7025587.1"
                     /translation="MKNNKNNGFLKNSFIYILLIIAVITTFQYYLKGTSSQNQQISYT
                     KLVKQLKAGEIKSISYQPSGGVVEVSGTYKKAKTIKSANSFTFLGGSVATKVTGFNSV
                     ILPNDSSIKSLVSAAEENNTNIQVKHESSSGTWISYIASFLPLVIMIGFFMMMMNQGG
                     GGGARGAMSFGKNKARSSSKDEVKVRFSDVAGAEEEKQELIEVVDFLKDPKRYKSLGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDA
                     KKAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNESIIVIAATN
                     RSDVLDPALLRPGRFDRKVLVGQPDVKGREAILRVHAKNKPLADNVDLKVVAQQTPGF
                     VGADLENVLNEAALVAARRNKKVIDASDIDEAEDRVIAGPSKKDRTISERERAMVAYH
                     EAGHTIVGLILSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKDDMKEQLAGLMGG
                     RVAEEIIFNAQTTGASNDFEQATAMARAMVTEYGMSEKLGPVQYEGNHAMMAGQMSPE
                     KSYSAQTAQLIDDEVRHLLNEARNKAADIINENRDTHKLIAEALLKYETLDAAQIKSI
                     FETGKMPETENDEDKARALSYDEIKEKMQEEDKK"
     gene            complement(2257..2799)
                     /gene="hpt"
                     /locus_tag="VBD27_001587"
     CDS             complement(2257..2799)
                     /gene="hpt"
                     /locus_tag="VBD27_001587"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEO7025588.1"
                     /translation="MLENDIKKVLYSEEDIILKTKELGAKLTADYAGKNPLLVGVLKG
                     SVPFMAELLKHIDTHVEIDFMVVSSYHGGTTSSGEVKILKDVDTNIEGRDVIFIEDII
                     DTGRTLKYLRDMFKYRQANSVKVATLFDKPEGRLVDIDADYVCYDIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEIYSK"
     gene            complement(2804..4078)
                     /gene="tilS"
                     /locus_tag="VBD27_001588"
     CDS             complement(2804..4078)
                     /gene="tilS"
                     /locus_tag="VBD27_001588"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO7025589.1"
                     /translation="MYNTILKDTLSKGLFTAHQKVLIAVSGGIDSINLLQFLYQYQKE
                     LSISIGIAHINHGQRKESEKEEEYIRQWGQIHDVPVFISYFQGIFSEDRARNHRYNFF
                     SKVMREEGYTALVTAHHADDQAETVFMRILRGSRLRYLSGIKQVSAFANGQLIRPFLP
                     YKKELLPNIFHFEDASNASSDYLRNRIRNIYFPTLERENNQLKDSLITLSEETECLFT
                     ALTDLTRSIEVTNCYDFLRQTHSVQEFLLQDYISKFPDLQVSKEQFRVILKLIRTKAN
                     IDYTIKSGYFLHKDYESFHITKIHPKTDSFKVEKRLELHNIQIFSQYLFSYGKFISQA
                     DITIPIYDTSPIILRRRKEGDRIFLGNHTKKIRRLFIDEKITLKDREEAVIGEQNKEL
                     IFVIVAGRTYLRKPSEHDIMKGKLYIENLEKR"
     gene            complement(4080..5366)
                     /locus_tag="VBD27_001589"
     CDS             complement(4080..5366)
                     /locus_tag="VBD27_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7025590.1"
                     /translation="MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKE
                     MYFDAIPERPTTKIEISSFQDEALTITGETLVPNTLLSIISLTINSNGIPVFTLSNGQ
                     FIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYSRVHLVE
                     QAETEHGIYYKTDSGFWISVEDLSVADNRMAKVQEVLLEKYNKDKYGIYIKQLNTQTV
                     AGINIDRSMYSASIAKLATLYASQEQVKLGKLSLDSKFEYKDNVNQFPNSYDPSGSGK
                     LEKKADHKLYTVKELLEATAKESDNVATNMLGYYVNNQYDSMFQTQVDTISGMHWDMK
                     KRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKNIPVKVAHKIGDAYDYKHD
                     AAIVYAEQPFIMIIFTDKSSYDDITKIADDVYQVLK"
     gene            complement(5366..5500)
                     /locus_tag="VBD27_001590"
     CDS             complement(5366..5500)
                     /locus_tag="VBD27_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO7025591.1"
                     /translation="MNNLIETIEHFLAYSDDKLEELADKNYQLRQTIVEKEKIESREK
                     "
     gene            complement(5503..5874)
                     /locus_tag="VBD27_001591"
     CDS             complement(5503..5874)
                     /locus_tag="VBD27_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEO7025592.1"
                     /translation="MSKPNVVQLNNQYINDENLKKRYEAEELRRKNRLMGWVLIFVML
                     LFILPTYNLVKSYRTLQERRQEVVKLTKDYQTLTNRTENQKLLAKQLKNPDYVQKYAR
                     AKYYFSKTGEMIYPLPDLLPK"
     gene            complement(5861..6133)
                     /locus_tag="VBD27_001592"
     CDS             complement(5861..6133)
                     /locus_tag="VBD27_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO7025593.1"
                     /translation="MRLDKYLKVSRIIKRRPVAKEVADKGRVKVNGVLAKSSTDLKLN
                     DQVEIRFGNKLLTVKVLEMKDSTKKEDAIKMYEIINETRIETDEQA"
BASE COUNT         1863 a         1169 c          891 g         2223 t
ORIGIN      
        1 attatactaa atatttctac ttcctgtcaa tactattttt gcattttttc ttttattttt
       61 aaaaagttaa tattatttat agtaactaac cttctatact tgtttaatgg atagcatttt
      121 taccgttgtc atgttcatat ttcatcttct taattcacaa atttaaactt catcttcata
      181 aaaaaacacc cctcaaattt tatctaaatt tgaagggtgt ttgaaattta taaagttctt
      241 taaaaatata tgatgactta ttttttatct tcttcttgca ttttttcttt gatttcatcg
      301 tatgataacg ctctcgcttt atcttcatca ttttctgtct caggcatttt acctgtctcg
      361 aaaatcgatt taatctgagc agcatcaaga gtctcatatt ttaagagggc ttctgcaatt
      421 aatttatgag tatcacggtt ttcgttgata atatcagctg ccttattacg tgcttcatta
      481 agaaggtgac gaacttcatc atcaataagt tgtgcagttt gagcagaata tgatttttca
      541 ggtgacattt ggccagccat cattgcatgg ttaccttcgt attgaactgg gccaagtttc
      601 tcactcatac catattcagt taccatagcg cgggccatgg cagtggcttg ttcaaagtca
      661 tttgaggcac ctgttgtctg agcgttgaaa ataatttctt ccgctacacg tcctcccata
      721 agacctgcta attgctcttt catatcatct tttgaaagaa gcatttgatc ttctttaggt
      781 aaagcaatca tatatccacc tgcacgacca cgtggtacga tagtaacttt atgaacaact
      841 cgcgcatttg aaagaatcaa accgacaatt gtgtgcccag cttcatggta agcaaccata
      901 gctctttctc tttcagaaat agtacgatct tttttagaag gaccagcaat tacacggtct
      961 tctgcttcat caatatctga agcatcaata acttttttat ttcgtcgcgc agcaactaaa
     1021 gcagcttcat tgagaacatt ctccaaatca gcaccaacaa atcctggggt ttgttgagct
     1081 actactttta agtcaacatt atctgctaat ggtttatttt tagcatgtac tctcagaatt
     1141 gcttcacgac cttttacatc aggttgtcca actagaactt ttctatcaaa acgtcctgga
     1201 cgcagtaatg cgggatctaa cacgtcacta cggttggtag cagcgataac gataatactt
     1261 tcgtttcctt caaaaccatc catttcaatt aaaagttggt tgagcgtttg ttcacgttca
     1321 tcgtttccgc cacccatacc agctccacgt cgacgtccaa cagcatcaat ttcatcaatg
     1381 aaaataatag cacgttcagc ttttttagca tcttcaaaca aagagcgtac tcgactagct
     1441 ccaacaccga caaacatttc aacaaaatct gaaccggaaa tactaaagaa tggaacccct
     1501 gcttcaccgg caacagcttt tgctaatagg gttttacctg tccctggagg tccttctaat
     1561 agaacccctg ctggaattcg ggcacctaga gatttatatc gtttaggatc tttaaggaag
     1621 tcaacgactt ctattaattc ttgcttttct tcctctgctc cagcaacatc cgaaaatcga
     1681 actttaacct catctttact agatgatctt gctttatttt taccaaagct catggcacca
     1741 cgagcgccac cgccaccgcc ttggttcatc atcatcataa agaaaccaat catgataact
     1801 aaaggtaaaa aactggcaat ataagaaatc catgtcccac ttgaactttc atgtttgacc
     1861 tgaatattag tgttattttc ttcagcagcg ctaacaagag attttattga agagtcattt
     1921 ggtagtataa cagaattaaa tcctgtaact ttagtagcaa cactaccacc taaaaaagta
     1981 aaactattag ctgatttaat tgttttggct ttcttatatg tcccactaac ttcaacaaca
     2041 ccaccacttg gctggtatga aatagactta atttctccag cttttaattg tttaacaagt
     2101 tttgtataag aaatttgctg gttttgagag ctagttcctt ttaaatagta ttgaaaggtt
     2161 gtaataaccg caataatcaa taatatgtaa ataaaggaat ttttcagaaa accattattt
     2221 ttattatttt tcatagttta agagagtacc ttttttctat tttgagtaaa tttcttcttt
     2281 taacacaccg acataaggta ggttacgata gttttcagca tagtctaaac cgaatcccac
     2341 aataaattca tttgggatat cgtaacaaac gtaatcagca tcaatatcta ccaatcgccc
     2401 ttctggctta tcaaaaaggg ttgcaacttt gacagagtta gcttgacgat acttaaacat
     2461 atctcgaagg tattttaatg tacgtccagt atcaatgatg tcttcaataa agattacatc
     2521 tcttccttca atgttagtat caacatcttt taaaatttta acttctccac tactagttgt
     2581 accaccatga tagcttgaaa caaccataaa atcaatttca acatgtgtat caatatgctt
     2641 taatagttca gccataaaag gcactgaccc ctttaataca cctactaata atggattctt
     2701 acctgcataa tctgcagtta atttagctcc taattctttc gttttcagaa taatatcttc
     2761 ttctgaatat aaaacttttt ttatatcatt ttcaagcatg atgtcacctt ttttctaaat
     2821 tttcaatata aagtttgccc ttcattatat catgttcaga aggttttctc aaatatgttc
     2881 tacctgcaac aatcacaaat atcaactctt tattttgctc cccaataaca gcttcttctc
     2941 tgtcttttag agttattttc tcatcaataa atagacggcg aattttttta gtgtgatttc
     3001 ctaaaaatat cctatcccct tcttttcttc ttcttaaaat aatcggagaa gtatcataaa
     3061 taggaatagt aatgtctgct tgagaaatga attttccata ggaaaataaa tattgtgaga
     3121 atatttgtat attatgcaat tctaatcttt tttcaacctt aaaactatcc gtcttaggat
     3181 ggatttttgt aatatgaaaa ctctcgtaat ccttatgaag aaaatagccc gatttaatag
     3241 tatagtcaat attggcttta gttctaatca attttaatat tactctgaac tgttctttac
     3301 tcacttgtaa atctggaaat ttgctaatgt aatcttgcaa taaaaattct tggacggaat
     3361 gagtttgcct taaaaaatca tagcagtttg ttacttcgat gctccttgtt aggtctgtta
     3421 aagccgtgaa taaacactct gtttcctcag agagagttat gagagaatct ttcaattgat
     3481 tattttctct ttccaaagta ggaaagtaga tgtttcttat tcgatttctc aaataatcag
     3541 acgaagcatt gctagcatct tcaaaatgaa aaatatttgg taataactcc tttttatatg
     3601 gaagaaatgg tctaatcaat tgaccatttg caaaagcact cacttgctta atccctgata
     3661 aatagcgtag cctgcttcct cttaaaattc tcataaatac agtttcagct tgatcatcag
     3721 catggtgagc tgttactaaa gccgtgtaac cttcttcacg cataactttt gaaaaaaaat
     3781 tgtagcgatg gttacgtgct ctatcttctg aaaatattcc ttgaaaataa gagataaaga
     3841 cagggacatc atgtatctgt ccccactgtc tgatatattc ttcttctttt tctgattctt
     3901 ttcgctgtcc atgatttatg tgtgcaatac caatactaat tgataactct ttttgatact
     3961 ggtataaaaa ttgcaacaaa tttattgaat caatgccacc agaaacagca attagaactt
     4021 tctgatgagc ggtaaataac cctttggata aggtatcttt caaaatagtg ttatacatat
     4081 tactttaaaa cctggtaaac gtcgtcagca atcttagtga tatcatcgta agatgattta
     4141 tctgtaaaaa taatcataat aaaaggttgt tctgcataaa caatagccgc atcatgtttg
     4201 tagtcataag catctccaat tttatgtgct actttaactg gtatattttt agggattctt
     4261 gtattatcaa aatcagtctt tgataggtaa ttaacaatat ctccattttg ataataaatt
     4321 gcttccatca ttttacccgc agcttgtgga gaaatctggc gtttcttcat atcccagtgc
     4381 ataccagaaa ttgtgtctac ttgtgtttga aacattgaat cgtattgatt attgacataa
     4441 taacctagca tatttgttgc aacattatct gattccttgg cagttgcttc aagtaactcc
     4501 ttaactgtat ataatttatg atcagctttt ttctctaatt tcccactacc acttggatca
     4561 tagctattag gaaattgatt aacattatct ttgtattcaa atttgctgtc caatgacagt
     4621 tttcctaatt ttacttgctc ttggctagca taaagtgttg ctaattttgc aatacttgca
     4681 gagtacattg acctatcaat attaatacca gctactgtct gtgtatttaa ttgtttgatg
     4741 tatataccat atttatcttt attatatttt tccaataata cttcttgaac cttagccata
     4801 cgattatcag caaccgatag atcttctact gatatccaaa aaccactgtc cgttttataa
     4861 taaataccat gttctgtttc tgcttgttct actaaatgca ctctggaata aggttttaaa
     4921 ttatttttaa cttcactaac accatttgtg taaggtgctt cataagtcac aaaagaaggt
     4981 tttaaccaat aatccaatga gacagattgc tgcttagaaa ctaaatcatt aaaaatagct
     5041 tcacgactag cctttatgaa ctgtccattg gacaaagtaa atactggaat tccatttgaa
     5101 ttaattgtaa gggaaataat cgacaataaa gtatttggaa ctaaagtctc tcctgttatg
     5161 gttaaagctt catcttgaaa actgcttatt tcaatttttg tagttggtct ctctggaata
     5221 gcatcaaaat acatttcctt atccacaact gtctgagtta aggcgtatcg tctattttga
     5281 gaaagcgtta atggctcctc ggtacttata gctgggattg atacaaaata taagctacat
     5341 aataagaagg ttaaaacttt cttcattact tttctctgct ctcaatcttt tctttttcta
     5401 caattgtttg cctaagttga tagttcttat cagctaactc ttctagtttg tcatccgagt
     5461 aggctagaaa atgttctatt gtttcaatta aattattcat aattattttg gtaaaaggtc
     5521 tggtaatggg taaatcattt cgccggtctt agagaaataa tacttcgctc gagcatattt
     5581 ttgaacgtaa tctggatttt ttagttgttt tgctagtaac ttctggttct cagttctatt
     5641 agttaatgtc tgatagtctt tcgttaattt tacaacttct tgacgacgtt cttgtaaagt
     5701 tctgtaactc ttaactaaat tataagtggg taaaataaat aaaagcatga caaaaataag
     5761 aacccaaccc attaaacgat tttttcggcg taactcctca gcttcgtaac gtttttttag
     5821 attctcatcg ttaatatatt gattatttaa ctgaacaaca ttaggcttgc tcatctgttt
     5881 ctatccttgt ttcattaata atttcataca ttttgatagc atcttctttc ttagtgctat
     5941 ctttcatttc taatacttta acggtcaaca atttattacc aaaacgaatt tcaacttggt
     6001 cattaagctt taggtcagtt gatgactttg ctaatacgcc attcaccttg acacgtcctt
     6061 tgtctgcaac ttctttagca acgggacgac gcttaatgat acgcgacact tttagatact
     6121 tgtctaatct cactgatgat cacatc
//
LOCUS       DATRQA010000110         6120 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.110, whole genome shotgun sequence.
ACCESSION   DATRQA010000110 DATRQA010000000
VERSION     DATRQA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.110"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(243..767)
                     /locus_tag="VBD27_001593"
     CDS             complement(243..767)
                     /locus_tag="VBD27_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7025594.1"
                     /translation="MVKDRQIQKTKVAIYNAFISLLQENDYSKITVQDVIGLANVGRS
                     TFYSHYESKEVLLKELCEDLFHHLFKQGRDVTFEEYLVHILKHFEQNQDSIATLLLSD
                     DPYFLLRFRSELEHDVYPRLREEYITKVDIPEDFLKQFLLSSFIETLKWWLHQRQKMT
                     VEDLLKYYLTMVER"
     gene            899..1762
                     /locus_tag="VBD27_001594"
     CDS             899..1762
                     /locus_tag="VBD27_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO7025595.1"
                     /translation="MTSNKKVAIAFILNISFSVLEFIFGSLFFSGAILADAVHDFGDA
                     IAIGISATLEKKSKKDEDTIFSLGYKRFSLLGALITSLILISGSILVMIENIPKLWHP
                     TPVNYHGMFILAVIAIIINGLASFILHSGQSKHEEILSLHFLEDILGWLAIIVISLIL
                     NWKPLYILDPLLSVAISTFILSKALPKLLSTLKLFLDGVPDSIDYAALHDELKGLSQV
                     RSINQLNIWSMDGIDNRAIIHCCLNQLISEKDCKRAIRTICQHYKINDVTVEIDYSLR
                     EHQNHCKPLKN"
     gene            1847..2701
                     /locus_tag="VBD27_001595"
     CDS             1847..2701
                     /locus_tag="VBD27_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7025596.1"
                     /translation="MTDTMILNDNHKIPTLGLGTWMIDNDRVAQVVKDAIELGYRHID
                     TAQAYNNEKGVGVGIKESGITRESLFVTTKIAAEHKDYESATKSIDRSLEALGLDYID
                     LMIIHSPQPWKEWRETDKHFDQGNLEAWRALEDAQKSGKVKSIGVSNFLEKDLENILK
                     NGYVKPAINQILAHIGNTPFDLIDYCQSKGIQVEAYSPIAHGQALKSDGIQKMAEKYG
                     VSVAQLCIQYLLQLNLIVLPKASSKEHLQSNLDFDFVISDEDMSILKSLMFDDYGEFS
                     NFPVFSGK"
     gene            2811..3838
                     /locus_tag="VBD27_001596"
                     /pseudo
     CDS             2811..3838
                     /locus_tag="VBD27_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            3854..4243
                     /locus_tag="VBD27_001597"
     CDS             3854..4243
                     /locus_tag="VBD27_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7025597.1"
                     /translation="MHIKEVSKKTGITSDTIRYYERINLIPPIHRSESGIREFTQNDI
                     DILEFVRYFRGAGVSVESLIDYIHLVEKGDSTIPARLEILKDKRNNLEDRLQGLQEAL
                     NRLNHKIDNYQKVLVPREQELFDPTTK"
     gene            4253..4648
                     /locus_tag="VBD27_001598"
     CDS             4253..4648
                     /locus_tag="VBD27_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO7025598.1"
                     /translation="MQETTKSIFPKGEKNPYGEFFIRQSYLAALAKSPDGNVSVGNVT
                     FEAGCRNNWHVHLDGYQILLVTEGSGWYQEENKKAVSLEPGNVIVTDKGVKHWHGAKK
                     DSEFAHIAITAGKSEFYEAVSLEEYSRLG"
     gene            4676..4993
                     /locus_tag="VBD27_001599"
                     /pseudo
     CDS             4676..4993
                     /locus_tag="VBD27_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660666.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            5080..5364
                     /locus_tag="VBD27_001600"
     CDS             5080..5364
                     /locus_tag="VBD27_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO7025599.1"
                     /translation="MLTIQEMKDTTFELQENFRRLNYPIKQVAKDLQLNISEVESLLS
                     LDVTYPGDVCMLRDYLEDMLKKEGKEVYPFSRLASHSANRWYPYETPWRY"
     gene            5603..6094
                     /locus_tag="VBD27_001601"
     CDS             5603..6094
                     /locus_tag="VBD27_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO7025600.1"
                     /translation="MLSFQIVEKPAMILAGVTLENVKSNQECPKLWEKLHKQYNPSKL
                     KEKGIQQAIGICKTQPDFRFDYSATYQVETSVQAPKGLEIIRIPSATYAVISVKGPMP
                     SSLQETWRKIIQGFFQENNLKPANSPNLEIYSSQHPQDTDYQMEIWLAIADITNNSKT
                     NLI"
BASE COUNT         2116 a          993 c         1074 g         1937 t
ORIGIN      
        1 gtgggaatta gcccactaga atataagcaa aagcctaaga caatatagta atcgaaaaat
       61 attcattttg cttatggtga catcaacaat cccaaagaaa ctctttcatc aaggggactt
      121 ttttatctcc cgaattatac tcatttaatc taataatttc aactcgctat attagacaat
      181 caatccattc atcacctctg ataacgctaa tgaaataaga gaatctccca actctctata
      241 atttacctct ctaccatagt taaataatat tttagtaaat cttcaacagt cattttctgt
      301 ctttgatgaa gccaccactt taaggtctcg ataaaactgg ataaaagaaa ttgttttaag
      361 aaatcttcag gaatgtccac ctttgtaata tactcctcac gcaatctagg gtaaacatcg
      421 tgttctaatt cacttctaaa gcgtaataaa aaatagggat cgtcagataa taataacgta
      481 gcgatactat cttgattttg ttcaaaatgt tttaagatat gaactaaata ctcttcaaac
      541 gtaacatctc taccctgctt aaataagtgg tgaaaaaggt cttcacagag ctccttgagt
      601 aacacttctt tgctttcata gtgagagtaa aaagttgatc ttcctacatt tgcaagtccg
      661 attacatctt gaaccgttat tttagaataa tcattttcct gtagcaagct tataaaagca
      721 ttataaatcg ctacttttgt tttctgaatt tgtctatctt ttaccatata ggacactttc
      781 taagttttgt tcagtattga acaagtgttc ttttttgttt ctagcaattc tcttttatct
      841 cctagataat agaagaaaga atcacactac aataccagta taacaaaagg agaaaaatat
      901 gacatcaaac aaaaaagtgg ctatcgcttt tatattaaat atcagctttt ccgtccttga
      961 attcatcttt ggaagtctat tcttctcagg agctatctta gccgatgctg ttcatgattt
     1021 tggtgatgca atagctattg gcatctcagc tactttagaa aaaaaatcta agaaagatga
     1081 agatacgata ttttcattag gttataaacg ttttagcctt ttaggagctc ttatcacaag
     1141 tctaattctt ataagtggat ctatcttagt catgatagaa aatattccaa aattgtggca
     1201 tcctactcct gttaattatc acggcatgtt tattctagct gtcatcgcta ttatcattaa
     1261 tggtctagct agttttattc tccattctgg tcaaagtaaa catgaggaaa tattaagcct
     1321 tcattttttg gaagatattc tagggtggtt agctattatt gttatatctc ttatcttaaa
     1381 ttggaaaccg ttgtatatcc ttgatccatt attatcagtt gccatatcta ctttcatctt
     1441 atctaaagca cttcctaaat tactatcaac cctgaaactt tttttagatg gtgttccaga
     1501 ttccatagat tacgctgcat tacatgatga acttaaggga ctttcgcaag taagatctat
     1561 caatcaactc aatatctggt caatggatgg tattgataat cgagcaatca ttcactgttg
     1621 cttaaatcaa ttgatatctg aaaaagattg taaaagagct attcgaacga tttgccaaca
     1681 ttataagata aacgatgtta ctgttgaaat cgattattct ttaagagaac atcaaaatca
     1741 ttgcaaacca ttaaaaaact aacctgagtg ttagtttttt ccttgacttg gagttaactc
     1801 taagtgttaa gattaggtca atactaatga aagaaggaaa ataaagatga cagatacaat
     1861 gatattaaat gacaatcata aaataccaac tttaggttta ggtacgtgga tgatagataa
     1921 tgatagggta gctcaagttg ttaaagatgc tatcgaatta ggctaccgcc atattgatac
     1981 tgcacaagca tacaacaatg aaaaaggggt cggtgtaggg attaaagaat ctggtattac
     2041 acgtgaatct ttatttgtta cgactaaaat tgccgctgaa cataaagatt atgaaagtgc
     2101 tacaaaatca attgatcgat cactagaagc tttagggctt gattatattg atttaatgat
     2161 tattcatagt ccacagcctt ggaaggaatg gcgtgaaaca gataaacatt ttgaccaagg
     2221 aaatttagaa gcttggagag ctttagagga tgctcaaaaa tctggtaagg taaaatccat
     2281 cggtgtctca aactttttag aaaaagactt agaaaatatc ttaaaaaatg gttatgttaa
     2341 acctgctatc aatcaaatct tagctcacat cggaaataca cctttcgact tgattgatta
     2401 ctgtcaaagt aaaggtattc aagttgaagc ttattcacca attgcacatg gacaagcatt
     2461 gaaatctgat ggtattcaaa aaatggctga aaaatatggg gttagtgttg ctcaattatg
     2521 tattcaatat ttactacaat taaacctgat cgttttacca aaggcttcta gtaaagaaca
     2581 tttacagtct aatctagact ttgactttgt aattagtgac gaagatatgt cgatattaaa
     2641 atcacttatg tttgacgatt atggggaatt ttctaacttc cctgttttta gtggaaaatg
     2701 aaaccatatg cggattttta cagatgttta attttcaaaa tttttaagtt tttcttgact
     2761 tgaagttaac tctaagagtt ataatgaagt tatagaaaag aggtaaggtt atgaaagtag
     2821 ctacatttat tgaacctggc aaaatggtta taactgatac accaaaacca gtcattgaac
     2881 aagagacaga tgctgttatc aaaattgtta gagcctgtgt ttgtggttca gacttgtggt
     2941 ggtatcgtgg tatttcaaaa cgtgaaagtg gttcttttgc aggtcatgag gctattggta
     3001 tcgttgagga agttggtact aaagtaactg acgtgtcaaa aggtgatttt gttattgttc
     3061 cctttacaca tggctgtggc caatgtccgt cttgtaaggc tggatttgat ggaaattgta
     3121 caaatcatca agctgaaaaa aatgtagata ccaaggtcaa tatctacgtt atactaatgc
     3181 aaactgggca ttggttaaaa ttcctggtca accttctgac tatgataatg agacacttaa
     3241 ctctcttctc accttatcag atgtaatggc aactggttat catgctgcag aagttaaaga
     3301 aggtgatacg gtagttgtca tgggagatgg tgctgttgga ctctgtggtg ttattgctgc
     3361 taaaatgtta ggtgctaacc gtatcattgc aatgagtcgt cacaaagata ggcaggaact
     3421 agcattgact tttggtgcaa cggatattgt cgaagaacgg ggtgacgaag ccgttaaacg
     3481 tgttttagat ttaaccaatc aagcaggtgc cgatgctatt ttagaatgcg ttggtacaga
     3541 gcagtctgtt gatacagcta cccaaattgc taggcctggt gctgtcatag gacgtgttgg
     3601 tatcccacaa aatccagaca tgaataccaa taatttattc tggaaaaata tcggccttag
     3661 aggtggcatt gcttctgtaa caacatttga taagtctgtc cttttagatg ctgttctaac
     3721 tcataaaatt aacccaggct tagtttttac aaaatccttt gtattagatg atattcaaaa
     3781 ggcttatgaa gcaatggata aacgtgacgc tatcaaatcc ttagtgattg ttgactgaat
     3841 atcgatgtta aaaatgcata tcaaagaagt tagtaaaaag actggcatta cgtcagatac
     3901 cattaggtat tatgagagaa ttaatcttat tccccctatt catcggagtg aatcaggaat
     3961 tcgtgagttc acacaaaacg atattgatat tttagaattt gtccgctatt ttagaggcgc
     4021 aggtgtttcc gttgaaagtc tcattgatta tattcatttg gttgaaaaag gcgattccac
     4081 aatcccagct aggctagaaa ttttaaaaga taaacgcaac aaccttgaag ataggcttca
     4141 aggcctccaa gaagcactta atcgtctaaa tcacaaaata gacaattatc aaaaagttct
     4201 tgttcctaga gaacaagaac tttttgatcc aacaacgaaa tgaggaaatc ttatgcaaga
     4261 aacaactaag tctatctttc ctaagggtga aaaaaatcca tatggtgaat tctttattag
     4321 gcaatcctat ctcgcagcat tagcaaaatc tccagatggg aatgtttcag ttggaaatgt
     4381 aacctttgaa gctggctgtc gtaataattg gcatgttcat ttggacggtt atcaaatcct
     4441 cctcgttaca gaaggttcag gttggtacca agaagagaat aaaaaggcag tttcattaga
     4501 accaggtaac gtgattgtaa ctgataaagg cgttaaacat tggcatggtg ctaaaaaaga
     4561 ttctgaattt gcacatattg cgattacagc tggtaagagt gagttttatg aagctgttag
     4621 tcttgaagaa tattcaagac taggttaaat tcaagtaagg aggaatcaat ccattatgaa
     4681 agaaaaacaa acagccggac gtcgtcaatt agaagaattt gcacctgaat ttgccagata
     4741 taatgatgat attttatttg gtgaggtttg ggcaaaagag gatcatttaa cagataaaac
     4801 acgttctatt attactatat ctgccttaat tagctgagga aatcttgaac agttagagca
     4861 tcatcttcaa tttgccaaac aaaacggagt tactaaagaa gaaattgctg acattatcac
     4921 tcacctcgct ttttatgttg gatggccaaa agcttggtca gcctttaata aagctaagga
     4981 aatttggata taaaataaca gacatagcaa gggagatact ttcccaaaag ccaaaaagaa
     5041 agatcaacca attattaaac caactattag gagaatgata tgcttactat tcaagaaatg
     5101 aaagatacaa cctttgaatt acaagaaaat tttagaaggt taaattatcc tattaaacaa
     5161 gttgctaaag acttgcaact taatatcagt gaagttgaat ctcttttaag tcttgatgtt
     5221 acttatcctg gagatgtttg tatgctgaga gattatctag aagatatgtt gaaaaaagag
     5281 ggtaaagaag tttatccatt ttctagatta gcatctcatt cagcaaatcg ttggtaccct
     5341 tatgagacac catggcgtta ttaaagacta aagattaatc catcatcttt agagaaataa
     5401 acaaaaatag tcaatagatt ctttttagaa aaaagctata aaataagtgc ctcttagaga
     5461 atactctata tcataagatt tcttctttga accatatcat tccaaaagaa aattatgatt
     5521 gctaacaaga caattattct aaaaagaaac tattttaacg actctataat tacttaagga
     5581 atcataaaga ataaagattt ttatgctatc atttcaaatt gttgaaaagc cagcaatgat
     5641 acttgctgga gtaactttag aaaatgtaaa gtcaaatcaa gaatgtccta aactttggga
     5701 aaaattacat aaacaatata atccatcaaa actaaaagaa aaaggtatcc agcaagccat
     5761 tggcatttgt aaaacacaac ctgattttcg tttcgattac agtgccactt atcaagtaga
     5821 gacaagtgtc caagccccaa aagggttaga aattattagg atcccaagcg ctacctatgc
     5881 agtcatttct gttaaaggtc caatgccatc gagtcttcaa gaaacatggc gaaaaataat
     5941 acaaggattt tttcaagaga ataatctaaa accagctaac tcgccaaact tagaaattta
     6001 ttcatctcaa catccacaag atactgatta tcaaatggaa atttggcttg ctatcgcaga
     6061 tattactaat aactcgaaaa caaacttaat ctagtattgc ttaatataaa aaagcccctt
//
LOCUS       DATRQA010000111         6106 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.111, whole genome shotgun sequence.
ACCESSION   DATRQA010000111 DATRQA010000000
VERSION     DATRQA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.111"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..640
                     /locus_tag="VBD27_001602"
     CDS             <1..640
                     /locus_tag="VBD27_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEO7025601.1"
                     /translation="KNYHLIEDGYNCFQAKLFLGGSVVKRVIKTYLFKKYVPYGFSKY
                     CLSIEVNSLVGLPHDIRSKKYKELPRKKLFDSLNKEQKSLIFKIFKTKPLTITPKSVL
                     LLTQPLAQDKCYKTPTERFQSIQEQYDYFDDIVQEYRTLGYNVYLKVHPRDVVDYSKL
                     PVELLPSNVPMEIIELMSTGRFECGITHSSTALDFLTCVDKKITLVDLKDIK"
     gene            637..2037
                     /locus_tag="VBD27_001603"
     CDS             637..2037
                     /locus_tag="VBD27_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO7025602.1"
                     /translation="MNTKKLLQSGFIYTLGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMNIIMLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIILLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYASYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVFGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSVPLIF
                     HLLGHQLLGQLDRIMLARLYNTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EKLLQLSTYISRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALLI
                     FHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLDNIVIRWSLGIIILIVYSIV
                     FQKVILDLLSKKRRRR"
     gene            2037..3062
                     /gene="neuB"
                     /locus_tag="VBD27_001604"
     CDS             2037..3062
                     /gene="neuB"
                     /locus_tag="VBD27_001604"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEO7025603.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLPEL"
     gene            3139..4293
                     /gene="neuC"
                     /locus_tag="VBD27_001605"
     CDS             3139..4293
                     /gene="neuC"
                     /locus_tag="VBD27_001605"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEO7025604.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIVLEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETPSVLEG
                     LSKLNEVTNFDNPYYKENASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            4290..4919
                     /locus_tag="VBD27_001606"
     CDS             4290..4919
                     /locus_tag="VBD27_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO7025605.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            4930..>6106
                     /gene="neuA"
                     /locus_tag="VBD27_001607"
     CDS             4930..>6106
                     /gene="neuA"
                     /locus_tag="VBD27_001607"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEO7025606.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPGLFTTLSDKGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSRTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLENYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGHSVEEIFSNFQKLYSLLAENKIKMRLTT
                     IAYTLFRETVNNADIEKINQWLTEFCYQNQIPLLDINRFLSKDGNLNYHLTSDGLHFT
                     "
BASE COUNT         1976 a          920 c         1139 g         2071 t
ORIGIN      
        1 taaaaattat catctaattg aagatggtta taactgtttt caagccaagt tattcttggg
       61 tggttctgtc gtaaaacgag taattaaaac ttacttattt aaaaagtatg ttccgtatgg
      121 tttttcaaaa tattgtctct caattgaggt taattcttta gtggggttac ctcatgacat
      181 aaggagtaag aaatataaag aactaccgag aaaaaaatta tttgatagtc ttaacaaaga
      241 acaaaaatca ctgattttca aaatatttaa aacaaaacca ttaactataa ctccaaagtc
      301 agtattattg ttgacacagc cacttgcaca agataaatgt tataaaacac ctacagagag
      361 gtttcaaagt attcaagagc aatacgatta ttttgacgat attgtccagg aatatagaac
      421 gttagggtac aatgtttatt taaaagttca tcctagagat gtagtagatt attccaaatt
      481 gccggtagag ctattaccat caaatgttcc tatggaaatt atagagttga tgtcaacagg
      541 tcggttcgaa tgtgggataa cacattcgtc cactgcgctg gattttttaa cttgtgttga
      601 taaaaaaata actttagtag atctgaagga tattaaatga atactaagaa attacttcaa
      661 tctggtttta tttatacact cgggaattta ttggtgcaag gattagcctt tatcacactt
      721 cccatctata ctcgggttat ctcaacagaa gtttatggcc aatatagttt atatgtcgct
      781 tggatgaata ttattatgct ttttattggc cttcagacga gcggttcttt aagttcagcc
      841 cgggtcaaat acggagaaga atttaaaagc tattcaggga gtgccttttc tgtaggtaat
      901 atatggtttc ttattatact tttgatagct tttctattta gaagttttct tgcaccatta
      961 gttggttttt ctgaatctat ttttttatta atggtgtgtc aaagttacgc tagctatgtg
     1021 gtgactttct ttggtcagta ttttatacaa caacagagga gtttggctaa tttaatatta
     1081 tccttagcca atgcagtttc atctgttgca ctatctctat ttttaatttt tcattggtcc
     1141 gatgactttt tatctagggt ttttggagct tttgttccta ctataataac tggaatagtt
     1201 gcctttgctt atatttatta tcatagcaaa tctttttaca atcctaagta ttttcggttc
     1261 attgtcactg tgtctgttcc cttgattttc catttgttag gacaccagtt gttggggcaa
     1321 ttagatagaa ttatgctagc tcgtttatac aatactaaag aagttgcaat gtatagtttc
     1381 ggttattcgc ttggaatgat tattcagatt gttttgaata gtattaatat ggcttggatt
     1441 ccatggtttt ttgatgctag aaaagaaaaa ttactgcaac tttcaactta tattagccgt
     1501 tatctctact taggagtttt cttaacatta ggttatttga ctgtttttcc tgagttagct
     1561 caaataatgg gaggagacaa gtatagtagt agtgttcaat ttatttcttt aattattgtc
     1621 agctactttt tagtttttct ctatacattt cctgtaaata tccaattctt ctatgctaat
     1681 acgacatgga ttcctattgg gacattatta gctgctggtg taaattggtt actaaacctt
     1741 gtattaattc ctcattatgc agcctatggc gctgctatgg ctacaataat atcttattta
     1801 gccttgttga tttttcatca tattgtttcg aaagttaaat accattattc agatgtctca
     1861 gttaggcagt acatcatttt aagtggtata gtatttagtt atgctatgtt aatgaacatg
     1921 ttcctagata atatcgttat tagatggagt ttaggaataa ttattcttat agtatatagc
     1981 attgtttttc aaaaagtcat tttagactta ttgagtaaaa aaagaaggag aagataatgg
     2041 tttatattat tgcagagatt ggttgcaatc ataatggaga tattaatctt gcgaaaaaaa
     2101 tggtagatgt tgccgtgtct tgtggtgttg atgctgttaa atttcagact tttaaagctg
     2161 agaaacttat ttctaaattt gctcccaaag ctgaatatca aaaagcaact acaggaacag
     2221 cagacagtca acttgagatg acgaaacgtt tagagttaag ctttgaagaa tacttagaaa
     2281 tgcgtgatta tgcaatttca aaaggtgtgg agaccttttc aacacctttt gatgaagagt
     2341 cattagagtt cttaatttct acagatatgc caatttacaa aattccatca ggagaaatca
     2401 ctaatttacc ttacttagaa aagattggca agcaacaaaa gaaagttatt ctttcgacgg
     2461 gtatggcggt aatggaagag atccatcaag cggtgaatat tttacgtcag aatggtacaa
     2521 ccgacatttc tattttacat tgtacaacag agtacccaac accttacccc tctctaaatt
     2581 taaacgttat tcatactttg aaagatgaat ttaaagattt aacgataggt tattcggatc
     2641 attcaattgg atcagaagta cctatcgcag cagcagcaat gggtgcagaa gttattgaaa
     2701 aacactttac tttagatact aatatggaag gtccggatca taaagccagt gcaacacctg
     2761 atattttagc tgctttagtt aaaggggttc gcattgttga acaagcctta ggtagatttg
     2821 aaaaaatccc agatccagta gaagagaaaa ataagattgt tgctcgtaaa tcagtcgttg
     2881 ctttaaaacc aattaaaaaa ggcgatattt attcaataga aaatattacg gtgaagcgcc
     2941 caggtaatgg tatttctcct atgaactggt atgacatctt gggacaagaa gcgcaagatg
     3001 atttcgaaga ggatgaagtt attcgtgatt cacgctttga aaatcaattg cccgagttat
     3061 aataacacct aattataatc ggcaacagat gtattacacg gctaagatta tgtaattact
     3121 atatatagaa gggatacgat gaaaaaaatt tgtttagtaa ccggatcgcg tgctgaatac
     3181 ggaataatga agccattgat tcaaagatta tcaaaagata aagaagtcaa cttacaaatt
     3241 attgcaacag caatgcatct ggaagaaaag tacggctata cttatcgtca aattgaagaa
     3301 gacggttttg atattgctta taaagttccc ttacatcttt atgatactga cagaagaact
     3361 gtatctactg caatggcgca tttacaacta ggattgacca aaatttttga caaggaagac
     3421 tatgatctag tcatcatttt aggggatcgt tacgaaatgt taccagttgt gaatgtagcg
     3481 ttgatttata atgtcccagt atgccacctt catggagggg agacctcatt aggcaatttt
     3541 gatgagtata ttcgccatgc aattactaag atgagtcacc tacacttagt ctctacagag
     3601 gactttcgtc aacgtgtgat tcagatggga gaacaacctc aatttgtaat taacacagga
     3661 gctctcggag tggaaaatgc tctatcaatc ccttctctaa ccaaagaagc aatagaaaag
     3721 caattaggta ttgtactaga ggaatcctat tttgttgttc tctatcatcc tgttactttt
     3781 gaacaaggaa aaagtgctgg agaacagatg aaagcggtac tatcagccct ttctaaattt
     3841 ggtgtccagt gtctatttat aggatccaat tcagatactg gttctgatga tattgcaaag
     3901 gctatcaata cttatttgat aaatcatgaa aatagttatt gctttgcttc tttatcaacc
     3961 caactttatc attccttaat tcgtcattca ctaggactga ttggtaattc gtcttctggt
     4021 ttaatagaag ttccttcttt gatgaaacct actcttaata ttggtgatcg ccaaaaaggt
     4081 aggctacatg gagaaagtgt agtaagtgta ccagtcgaaa ccccctctgt attggaggga
     4141 ttatcaaaat tgaatgaagt gaccaacttt gacaatccct actacaaaga gaatgcttca
     4201 tctattgcat atgaagcaat aaagctttat ttgaaagatg aaccttcaat ttcccaacca
     4261 ttttatgatt taaaggagaa taatctaaaa tgaaaaaaaa gttggcattt ttaggcgcag
     4321 gaagtcatgc tgatgcgatt gctccggtta ttgatcctct tgtgtatgat ttcgtaggtt
     4381 tttttgatga taaagatatt acggagcatg atggttatcc tgttcttgga aaactttatg
     4441 atgtgctacc ttaccttgaa gatggctcaa tagatgcagt atttattaca ataggtgata
     4501 atgctaaaag gaaagaacta tttgaatatg tagcaaagga ttattatgac tttattatta
     4561 acatcattag tcccaatgct ttagtattga caccagatag tatttgtggg cgtggtatct
     4621 ttattggttt tggggctttt ataggttcta aagtgaagct gtttgataac aacgttgtta
     4681 atacaggagc gctcattgaa catcatactg ttgtagaatc acactgtaat atagcaccta
     4741 atgctaccat aaatggtctt tgttatatta gagaagaagt ttatgtaggt agtgccagtg
     4801 ttattattca aaccttggat atttcatcgt gtacgacaat tggcgcagga gctgttgttg
     4861 taaaagatat tatagaacct ggaacctatg taggggttcc agccaaaaaa ataaaataaa
     4921 ggatatgtta tgaagccaat ttgtattatt cctgcgcgat cagggtcaaa aggattacca
     4981 gataagaata tgttattcct agcaggtaaa cccatgattt tccatactat agatgctgca
     5041 attgaatctg gaatgtttga taagaaagat atttttgtta gtacagattc agaattgtac
     5101 agagagattt gtttagaacg cggtatttca gtggtgatga gaaaaccgga actttcaact
     5161 gatcaggcaa cttcgtatga tatgttaaaa gattttttat ctgactatga agataatcag
     5221 gagtttgtgt tacttcaagt aacctctcca ctaagaaaat catggcatat aaaggaagca
     5281 atggagtatt attcttcaca tgatgttgac aatgttgtaa gtttttctga agttgagaaa
     5341 caccctggtc tgtttacgac attgtctgat aaaggctatg ctatagatat ggtgggagca
     5401 gataaaggtt atcgtcgcca agatttacaa cctttatact atccgaacgg cgctattttt
     5461 atttctaata aagaaactta cttaagggaa aaaagctttt tcacctctag gacatatgct
     5521 tatcaaatgg caaaggaatt ttcattagat gttgatacga gagatgattt tatccacgtc
     5581 atcggtcacc tctttttcga ttatgccatt agggaaaaag agaataaagt tttttataaa
     5641 gaaggctata gtcgtttgtt caatagagaa gcttcaaaga taattttagg tgattcaaaa
     5701 acgatatcta tctcactaga aaattaccat aattattccc aaggtggtgt aacattagca
     5761 acgatgttgg aaaacttacc taactttttg acagctaatg taacagaggc ttttgtttct
     5821 ataggggtaa atgatctcat tacaggtcat agtgtggaag aaatattcag caatttccaa
     5881 aaactctact cattattagc tgagaataaa ataaagatga gattgacaac tattgcttac
     5941 acccttttta gagaaactgt caataatgca gatattgaga aaatcaatca atggctaaca
     6001 gaattttgtt atcaaaatca gattccactg ttagatatta atagattttt atcaaaggat
     6061 ggtaatctaa attatcattt aactagtgat ggattacatt ttactc
//
LOCUS       DATRQA010000112         6092 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.112, whole genome shotgun sequence.
ACCESSION   DATRQA010000112 DATRQA010000000
VERSION     DATRQA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.112"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(35..1054)
                     /locus_tag="VBD27_001608"
     CDS             complement(35..1054)
                     /locus_tag="VBD27_001608"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO7025607.1"
                     /translation="MYLIEPIRNGKRITDGAIALAMQVYILQNVFLDDDILFPYYCDP
                     KVEIGKFQNAVIETNQEYLKEHDIPVVRRDTGGGAVYVDSGAVNICYLMKDHGQFGDF
                     KRAYEPAIKALKTLGASSVEMRGRNDLVIDGKKVSGAAMTIVNGRIYGGYSLLLDVDF
                     DAMEKVLNPNRKKIESKGIKSVRSRVGDIRSHLSEDYRHITTDQFKDLMVCQLLHIDH
                     IDQAKRYHLTEKDWAAIDALADEKYKNWDWNYGNSPQYSYHRDARFPSGTYDFHLEIE
                     KGIITNCRIYGDFFSSKDISDIENLLIGCPMKEELVLEKLSTLSLEDYFGQTSPEEIK
                     AVLFS"
     gene            complement(1081..1959)
                     /locus_tag="VBD27_001609"
     CDS             complement(1081..1959)
                     /locus_tag="VBD27_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEO7025608.1"
                     /translation="MTVWKTLEKTNHSQSEILSQLIEESDAIVVGIGAGMSAADGFTY
                     IGPRFEEAFPDFIAKYQLLDMLQASLYDFEDWEEYWAFQSRFVALNYLDQPVGQAYLD
                     LKDILAKKEYHIITTNADNAFAVADYNLEKVFHIQGEYGLWQCSQHCHQQTYRNDQAI
                     RQMIAQQKDMKIPSNLIPKCPKCDQPFEINKRNEEKGMVEDADFHAQRQRYENFLSQH
                     QNDKVLYLEIGVGHTTPQFIKHPFWRFVSLNENSLFVTLNHKHYRIPQKIRSRSVQLT
                     QHIAELIAEAKKEIIG"
     gene            complement(1928..2746)
                     /locus_tag="VBD27_001610"
     CDS             complement(1928..2746)
                     /locus_tag="VBD27_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEO7025609.1"
                     /translation="MPNQKQLLLAMIEYLQSEKLTDVDDLRTTDLQTVWRGLVNQQDP
                     QNISQEYLSLEDRYLSHWWNTQKVKTIDVCHQTVYSNVFTYHGDICLLQVDAIVNAAN
                     SKLLGCFIPNHHCIDNQIHTFAGSRLRLACHQLMTQQGRMEAVGQAKLTESYHLPCKY
                     VIHTVGPYVKVDQKPSRIREDLLKSSYKSCLQLAAQANLKTIVFPCISTGEFGFPNQR
                     AAELAVQEILEWQRENQHKLYIIFNTFTPKDQEIYQKLLLKEEIDDSVEDSRKN"
     gene            complement(2739..3071)
                     /locus_tag="VBD27_001611"
     CDS             complement(2739..3071)
                     /locus_tag="VBD27_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEO7025610.1"
                     /translation="MKKIANYLLIEKNEELYTISLTPELQDDLGTVGYVEFTDDTNLE
                     VDDVILNIEASKTVMAILSPLTGKVVKVNTAASQEPTLLNSEKADENWLVVLTDVDYA
                     AFEALENA"
     gene            complement(3100..4086)
                     /locus_tag="VBD27_001612"
     CDS             complement(3100..4086)
                     /locus_tag="VBD27_001612"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEO7025611.1"
                     /translation="MKLSVLDYGLIDYGKTASDAIQETILLSQEAEQLGYHQFWVAEH
                     HGVKAFSISNPELMIMHLANQTKSIKIGSGGIMPLHYSSFKLAETLKTLETCHPNRVS
                     IGLGNSLGTVKVSNALRSLHKAHDYEEVLEELKSWLIDESSSKEPLVQPTLSSFPDLY
                     VLGSGQKSAYLAAKLGLGFTFGVFPFMDKDPLTEAKKLSSLYYHQFEEYYPNKSPNLM
                     VAPFVVIADTSEEAENIAKTLDIWMLGNKDFNEFATFPTIEEANHYQLTPEQKAKIKS
                     NRHRMIVGDPKQVKESLDALVNASQAEELLLIPLVPGLDQRIKSLKLLSQLY"
     gene            complement(4083..5282)
                     /locus_tag="VBD27_001613"
     CDS             complement(4083..5282)
                     /locus_tag="VBD27_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEO7025612.1"
                     /translation="MNNVQGNLFRPLTLPNGLSLENRFVLSPMVTNSSTSEGFVTDDD
                     IAYAVRRAKSAPLQITGAAYITEYGQLFEYGFSVSKDEDIPGLTKLAKAMKSKGAKAV
                     LQLTHAGRFSSHTLARHGYVYGPSPMQLQSPYPHQVKELTHKDILRIIDEYVQATRRA
                     IQAGFDGVEISSAQRLLIQTFFSTFSNQRKDEYGPQTLTNRCRLGLEVFKAVQKVIRE
                     EAESDFILGFRATPEETRGSQIGYSIEEFMEFLEKILAIAQVDYLAIASWGHDVFRNT
                     IRSEGVYKGQLVNQVIFEHFGDRVPIMATGGINSASKVFEALQHAHMIGASTPLVVDP
                     EFLQKIKAKRSDQINLRIKVNDLEGLAIPKASFKDIVPLMDYGESLPKEAREVFRELR
                     SNYREER"
     gene            complement(5275..>6092)
                     /locus_tag="VBD27_001614"
     CDS             complement(5275..>6092)
                     /locus_tag="VBD27_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEO7025613.1"
                     /translation="QLPVSIFKDYVTDAQDAEKPFIWTEVFLREINRSNQEIILHIWP
                     MTKTVILGMLDRELPHLELAKKEIISRGYEPVVRNFGGLAVVADEGILNFSLVIPDVF
                     ERKLSISDGYLIMVDFIRSIFSDFYQPIEHFEVETSYCPGKFDLSINGKKFAGLAQRR
                     IKNGIAVSIYLSVCGDQKGRSQMISDFYKIGLGDTGSPIAYPNVDPEIMANLSDLLDC
                     PMTVEDVIDRMLISLKQVGFNDRLLMIRPDLVAEFDRFQAKSMANKGMVSRDE"
BASE COUNT         1924 a         1201 c          957 g         2010 t
ORIGIN      
        1 tatttttttg caaaaaaaat ttaaaatgtt cgtcttatga aaataaaact gcttttattt
       61 cttctggact agtctgacca aaataatctt ctaatgataa agtacttaat ttttccaaga
      121 ctaattcttc tttcataggg caaccaatga gaaggttttc aatatctgaa atatctttag
      181 aactgaaaaa gtcaccataa atacggcaat tcgtaataat acctttctca atttctaagt
      241 gaaaatcgta agttcctgac ggaaaacgag catctctgtg atacgaatac tgtggtgagt
      301 taccataatt ccaatcccaa ttcttatatt tttcatcagc tagagcgtca atcgcagccc
      361 aatctttttc ggttagatga tagcgttttg cctgatcaat atgatcaata tgtaataatt
      421 gacaaaccat taaatcttta aattgatcgg ttgtgatatg acgataatct tctgacaagt
      481 ggctacgaat atctcctact cgacttcgga cagattttat ccctttagat tcaatttttt
      541 tccgattagg atttaacact ttttccatag catcaaaatc aacatctaat aaaagagagt
      601 aaccaccgta aatacgaccg tttactatcg tcattgctgc acctgatact tttttaccat
      661 caataactaa gtcatttcgt cccctcattt caacactaga agcacctaaa gtttttaggg
      721 ccttaatagc tggctcataa gctcgtttga aatcaccaaa ttgtccatga tctttcatta
      781 gatagcaaat atttacagca cctgagtcaa cgtaaacagc tcctccacca gtatctcgac
      841 gaacaacagg aatatcatgt tctttcaaat attcttgatt tgtctctata actgcattct
      901 gaaattttcc gatttcaact ttaggatcac aatagtatgg aaataaaata tcatcatcta
      961 aaaaaacatt ttgtaaaata taaacttgca tggcaagtgc aatcgcaccg tctgtgatac
     1021 gcttaccatt tctaattggt tcaataagat acatttaggt ctccttttat agcaatttag
     1081 ttatccaata atttcttttt tcgcttcagc aattaattca gcgatatgct gtgtcagttg
     1141 cacactcctt gagcgaatct tttgaggaat gcgataatgc ttgtgattaa gagtaacaaa
     1201 taaagagttt tcatttaaag agacgaatcg ccaaaacgga tgcttaataa attgtggcgt
     1261 ggtatgtcca actccaatct ctagataaag aactttgtca ttttgatgtt gtgataagaa
     1321 attttcataa cgttgtcgtt gagcatgaaa gtcagcatct tccaccatac ctttttcttc
     1381 gttacgttta ttaatttcaa acggttgatc acactttggg catttgggga ttaagttact
     1441 aggaatcttc atatcctttt gttgtgcaat catttgacga attgcctgat catttcgata
     1501 agtttgttga tgacaatgtt gactacactg ccataagcca tattcacctt gaatatgaaa
     1561 aactttttct aaattataat cagccacagc gaaggcatta tcagcattag ttgtaataat
     1621 atgatattct tttttcgcta aaatatcctt taaatcaaga taagcttgtc ctaccggttg
     1681 atctaaataa ttaagtgcca caaaacgact ttggaaggcc caatattctt cccaatcttc
     1741 aaagtcataa agacttgctt gcaacatatc taatagttga tatttagcaa taaaatccgg
     1801 aaaagcttct tcaaatctgg ggccaatata ggtaaatcca tccgctgcag acatgcctgc
     1861 accaatacca actacaatag catctgattc ttctattaac tgacttagta tttctgattg
     1921 agaatgatta gttttttcta gagtcttcca cactgtcatc tatttcctct tttaataata
     1981 atttttgata aatctcttgg tctttaggtg tgaatgtatt gaaaataata tagagtttgt
     2041 gctgattttc gcgttgccac tctaatatct cttgaacagc caattcagcg gctctttgat
     2101 taggaaaacc aaattctccc gttgatatac aagggaagac aattgtttta agattagctt
     2161 gagctgccaa ttgtaagcag gatttataag aagactttaa taaatcctct cttatacgag
     2221 atggcttttg atcaactttt acataaggtc cgactgtatg aataacatac ttacaaggaa
     2281 gatgatagga ttcggtaagt ttagcttgtc ctacggcttc cattcgtcct tgttgtgtca
     2341 tcaattgatg acatgctaaa cgtaatcgtg aacctgcaaa cgtatgaatc tgattgtcaa
     2401 tacaatggtg attaggaata aaacaaccta acaacttact atttgctgca ttaacaatag
     2461 catccacttg taataagcaa atatccccat ggtaggtaaa tacatttgaa tataccgttt
     2521 gatgacagac gtctatcgtt tttacttttt gagtattcca ccaatgtgac aaatacctat
     2581 cttctaaaga caggtactct tgactaatgt tttgaggatc ttgttgatta actagaccac
     2641 gccaaaccgt ttgcaaatcg gtggtcctca agtcatcaac gtctgttaat ttttctgact
     2701 gaagatactc aatcatggct agcaatagtt gtttttgatt aggcattttc taaagcttca
     2761 aaagcagcat aatcaacatc tgttaacaca accaaccaat tttcgtcagc tttttcagaa
     2821 tttaacaaag ttggttcttg acttgcagct gtattaactt taacaacttt acctgtaaga
     2881 ggagataaaa ttgccataac cgtctttgaa gcttcaatat ttaaaataac atcatcaact
     2941 tctaaatttg tatcatctgt gaattccaca tatccaacag ttcccaagtc atcttgaagt
     3001 tcgggagtta agctgatagt ataaagttct tcattttttt caattaataa gtagttcgca
     3061 atttttttca tgagtaattt cctttctgag aaaacgtgat taatataact gtgataataa
     3121 ttttaaggat ttgattcgtt gatcaagtcc tggaacaaga ggaatcaaaa gtagttcttc
     3181 tgcttgacta gcattaacta aagcgtcaag gctttcttta acttgttttg gatcacctac
     3241 aatcatacgg tggcgatttg atttaatttt tgctttttgt tcaggagtca attggtaatg
     3301 attagcttct tcaatagtag gaaatgttgc aaattcattg aagtctttat ttcctagcat
     3361 ccagatatct aaagtctttg ctatgttttc agcttcttct gaagtatcag ctataactac
     3421 aaatggagct accatcaaat taggagactt attaggataa tattcttcaa actgatgata
     3481 ataaagagat gaaagctttt tagcttctgt caatgggtct ttgtccataa aaggaaaaac
     3541 accgaaggta aagcctaagc caagtttagc cgctaaataa gctgattttt gaccactccc
     3601 caacacatat aagtctggga agctagaaag agtcggttga actaatggtt ccttactgga
     3661 tgattcatca ataagccatg acttcaattc ctccagtacc tcttcgtaat catgtgcttt
     3721 atgtaagcta cgaagtgcat ttgaaacttt aactgtccct agtgaatttc ctaaaccaat
     3781 acttactcga ttcggatgac atgtctctaa tgtcttgaga gtctccgcga gtttaaaact
     3841 actatagtgc agaggcatta tacctccaga gccaattttg atagatttag tctggttagc
     3901 caaatgcatt atcattaatt ctggattgct aatactgaat gccttaacac cgtgatgttc
     3961 agccacccaa aattgatgat agcctagttg ctccgcctct tgtgataaaa gaatcgtttc
     4021 ttgtattgca tcacttgcag tttttccata atcaataagc ccataatcaa ggacacttaa
     4081 tttcatcttt cctccctata attgcttcga agttctctaa aaacttctcg agcttccttt
     4141 ggtaaagatt ctccataatc catcaaagga acaatatctt taaaggaggc ttttggaatc
     4201 gccaaacctt ctaaatcatt cactttaatc cttaaattaa tctgatcaga acgcttagct
     4261 tttatttttt gtaaaaattc cggatcaact actaagggcg tggaagctcc tatcatatga
     4321 gcatgttgta aggcttcaaa cacctttgaa gcactattaa tacctccagt tgccataatt
     4381 ggaactctat caccaaaatg ttcaaatata acttgattaa ccagctgccc tttgtaaact
     4441 ccctcagaac gtatagtatt tctaaagaca tcatgccccc agcttgcaat ggcaagataa
     4501 tctacctgag caatcgctaa aattttttca agaaattcca taaactcttc aatgctatat
     4561 ccaatttgtg aaccgcgagt ttcttcaggc gtcgctctaa aaccaaggat aaaatctgac
     4621 tccgcttctt ctctgataac tttttgtacc gctttaaaaa cttctaagcc taaacgacag
     4681 cgattagtca aagtttgagg accatactca tcttttcttt gatttgagaa ggttgagaaa
     4741 aaagtttgaa ttagtaatcg ttgtgcagaa gatatttcaa caccatcaaa tccagcttgt
     4801 atagctcgtc ttgtagcctg gacatattca tcgataatcc ttagtatatc tttgtgtgtc
     4861 agttctttaa cctgatgtgg gtatggcgat tgcaattgca tagggctggg accatataca
     4921 tatccgtgtc ttgccaaagt gtgacttgag aaacgtccag catgcgtcag ctgcaaaaca
     4981 gctttcgctc ccttagactt cattgcttta gccaatttag tcaaaccagg aatgtcctca
     5041 tcctttgaaa cgctaaaacc atattcaaat aactgaccat attctgtgat atatgctgct
     5101 ccagtaattt gtaatggagc ggatttagca cgacgaacag catatgctat gtcgtcatca
     5161 gttacaaaac cttctgatgt agaagaatta gttaccatag gtgatagaac aaaacgattt
     5221 tctaagctca atccattggg aagtgttaat ggacgaaata aattaccttg gacattattc
     5281 atctctgctc accatcccct tattagccat agacttagcc tgaaatctat caaactctgc
     5341 aactaaatcg ggtctaatca tcagtaaacg atcattaaaa cctacttgtt taaggctaat
     5401 caacatacga tcaataacat cttctactgt cataggacaa tctaatagat cagatagatt
     5461 agccataatt tcaggatcta catttggata agcaattgga ctacccgtat cacctagacc
     5521 aatcttataa aaatctgaaa tcatttgact ccgccctttt tgatcgccac aaacgctaag
     5581 gtaaattgat accgcaatac cattctttat acggcgctga gccaagccag caaatttttt
     5641 gccatttata ctaagatcaa acttaccagg acaataggag gtctctactt caaagtgctc
     5701 aataggttga taaaaatccg aaaatatact tctaataaaa tcgaccatta taagataccc
     5761 atctgagata gacaattttc tctcaaaaac atctggaata accaatgaaa aatttaaaat
     5821 tccttcatca gctacaactg cgagacctcc aaaattccga acaactggtt cataaccacg
     5881 actgatgatt tcttttttag ctaattctaa atgtggtaat tctcgatcta acatccccag
     5941 aatgactgtc ttagtcatcg gccaaatatg caaaataatt tcttgatttg agcgattaat
     6001 ctcccttaaa aatacttctg tccatataaa aggtttttcc gcgtcttgag catctgtaac
     6061 atagtcttta aaaatagata caggtaactg cg
//
LOCUS       DATRQA010000113         6081 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.113, whole genome shotgun sequence.
ACCESSION   DATRQA010000113 DATRQA010000000
VERSION     DATRQA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.113"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..418)
                     /locus_tag="VBD27_001615"
     CDS             complement(<1..418)
                     /locus_tag="VBD27_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7025614.1"
                     /translation="MAAGPNIVMTRVDERLIHGQGQLWVKFLSCNTVIVANDDVSKDH
                     LQQTLMKTVVPESIALRFFDIQKVIDIIHKANPAQTIFIIVKDLKDVYRLVAGGVPIK
                     EINIGNIHNGEGKEQVSRSIFLGMKDKEIIRKLNQEY"
     gene            complement(473..1669)
                     /locus_tag="VBD27_001616"
     CDS             complement(473..1669)
                     /locus_tag="VBD27_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEO7025615.1"
                     /translation="MMKIKPVKVESIENPKRFLDSRLLTKIEVEEAIEKALKQLYINI
                     DYFGEEYPTPATFNNTYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFL
                     NRINNRIALDHHDLGFLYTPSCTAEYRINGDAKALEATIKAADKLMERYQEKGGFIQA
                     WGELGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAF
                     HTFYFDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMT
                     NYFLNRLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKHLPEVDPDKETYKY
                     AMHTMLRSLIEQYSNSEFIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYK
                     DWELYW"
     gene            complement(1672..2106)
                     /locus_tag="VBD27_001617"
     CDS             complement(1672..2106)
                     /locus_tag="VBD27_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7025616.1"
                     /translation="MIKIIIVAHGNFPDGILSSLELIAGHQEYVVGINFIAGMSSNDV
                     RVALQREVIDFKEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLS
                     RTMFEHVDDLVDKVITSSHEGIVDFSTCLATQTAEATFEGGI"
     gene            2384..3196
                     /gene="dhuD"
                     /locus_tag="VBD27_001618"
     CDS             2384..3196
                     /gene="dhuD"
                     /locus_tag="VBD27_001618"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric 2-dehydro-3-deoxy-D-gluconate
                     5-dehydrogenase DhuD"
                     /protein_id="HEO7025617.1"
                     /translation="MTEQFLKDNFSLEGKVALITGASYGIGFSIATAFARAGATIVFN
                     DIKQELVDKGISAYKKLGIKAHGYVCDVTDEDGINEMVNKISQDVGVIDILVNNAGII
                     KRTPMLEMSAADFRQVIDIDLNAPFIVSKAVLPGMIQKGHGKIINICSMMSELGRETV
                     AAYAAAKGGLKMLTKNIASEYGSANIQCNGIGPGYIATPQTAPLRERQDDGSRHPFDQ
                     FIIAKTPAARWGEAEDLGAPAIFLASDASNFINGHILYVDGGILAYIGKQPQ"
     gene            3213..3851
                     /gene="dhuI"
                     /locus_tag="VBD27_001619"
     CDS             3213..3851
                     /gene="dhuI"
                     /locus_tag="VBD27_001619"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase DhuI"
                     /protein_id="HEO7025618.1"
                     /translation="MKIALINENSQASKNTLIYKELKAVSKKKGFDVFNYGMYGKEEE
                     SQLTYVQNGLLTAILLNSGAADFVITGCGTGIGAMLACNSFPGVVCGFAADSVDAYLF
                     SQVNGGNALSLPFAKGFGWGAELNLRYLFERLFEDEKGGGYPKERAVPEQRNARILSE
                     VKQITYRDLLSVLKEIDQDFLKETISGEHFQEYFFANCQNQNIADYLKSVLD"
     gene            3877..4884
                     /locus_tag="VBD27_001620"
     CDS             3877..4884
                     /locus_tag="VBD27_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO7025619.1"
                     /translation="MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNIARALQ
                     GFGQDTKLLSALPNNPIGNSFLQFLKAQGIDTHSIQWVGERVGLYFLENSFACRKGEV
                     VYDRDYSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKEAKKREI
                     TISLDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDI
                     KERMISLINHFDFQVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGS
                     GDAFVAGALYQLLQHSDSKTVIDFAVASASLKCALEGDNMFETVTAVNKVLNQSKDII
                     R"
     gene            4896..5534
                     /locus_tag="VBD27_001621"
     CDS             4896..5534
                     /locus_tag="VBD27_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO7025620.1"
                     /translation="MIKSKILLQLMQGKIVAVIRGNSQEEAFQAAQACIKGGISAIEI
                     AYTNSKASQVIEQLVTQYTNQEQVVVGAGTVLDAETARMAILAGAKFIVSPAFNLQTA
                     KLCNRYAIPYLPGCMTLSEITTALEAGCEIIKIFPGGTLGTSFISSLKAPLPQVQIMV
                     TGGVNLTNAKDWFLSGVTAIGIGGEFNKLAALGEFDKITEMAKQYKIQLNLN"
BASE COUNT         1908 a         1176 c         1040 g         1957 t
ORIGIN      
        1 gatattcttg gtttaatttt ctaattatct ctttatcctt catccctaag aagatagagc
       61 gacttacctg ctctttacct tctccattat gaatatttcc aatattaatc tctttaattg
      121 ggacgcctcc tgctacaagt ctatagacat ctttcaaatc tttaacaatt ataaaaatcg
      181 tctgtgctgg gttggccttg tgaataatat caataacttt ttgaatatca aaaaatcgca
      241 atgcaattga ttctggcacc accgtcttca ttaacgtttg ttgaagatga tctttggaga
      301 catcatcatt tgccacaata acagtattac aacttaaaaa tttcacccaa agttgtcctt
      361 gtccatgaat aagacgctca tcaacacgtg tcattacaat atttggtcct gctgccattt
      421 ctgacctcct tgttttctat tatttattgt ttattttgtt agtaaaatac tcttaccaat
      481 aaagttccca gtctttatag aatcttatta aggcttctaa gtaataataa tctccccaaa
      541 tattaccttc atctactcct ttacctgaat gccacgaata cacaccgtgc aatagaagag
      601 gacgtcctgc tataaattca ctattactat actgttcaat cagactacga agcattgtat
      661 gcatagcata tttgtatgtc tctttatcag gatctacttc tggtaaatgt ttaagcatct
      721 catgaatccc acacacagct gttgctgttg cggatgtatc tctaggctga cccgagccat
      781 ccgtaaaaat aaggtcccaa taggatacct tgtcttcagg taaacgattt agaaaatagt
      841 ttgtcatacc tttaaaaagg ataatctgct gataatcttt cattttccgg taactaagcg
      901 gaataccgta gatgccccat gcttgccctc ttgcccaaga tgactcatca ctataaccct
      961 gtcgtgtgac accttttaac ggttctccag tttctgggtc gaaataaaaa gtatgaaaag
     1021 cagaagaatc atcacgcacc acattgttag ctgaagcgta gaagtgattc accgcaactt
     1081 gtctatactt ttcatcacct gtctgttcat aagcaaaaaa taagagttgg atattaagta
     1141 agcaatcgat aattaagcga tagtgttcct tatacccgag ttctccccaa gcctgaataa
     1201 atccaccttt ttcttgatag cgctccatca atttatctgc agcttttata gtggcttcta
     1261 aagctttagc atcaccattg atacgatatt ctgctgtaca agatggtgtg taaagaaatc
     1321 ctaagtcgtg gtgatctaat gctatacgat tattaatacg atttagaaat gacaatacat
     1381 ttttgtgggc tatgtttttt aactttttat cctgattata ctcataagct aaccacaagc
     1441 accctgtcca aaaaccattt gtccattctg tgttatccat aactttataa gtattattga
     1501 atgttgcagg cgttggatac tcttcaccaa agtaatcaat attaatataa agttgcttca
     1561 aggctttttc aatggcttcc tcaacttcaa tcttagttaa taatctactg tctaaaaaac
     1621 gctttggatt ttcaattgac tcaaccttga ccggttttat tttcatcatt attaaatgcc
     1681 tccctcaaaa gttgcttctg cagtttgtgt agcaaggcaa gtagaaaaat caacaattcc
     1741 ttcatgagat gatgttataa ctttatcaac aaggtcatct acatgttcaa acatcgttct
     1801 agataaaaca gcttccatta acattgaaag gttcaagccc gataaaactt ttatcttttt
     1861 atctgtatat tctactgata aggcggaaga aacattgaaa ggagtcccac ctagcaaatc
     1921 agtcaatact aaaatttctt tgaaatcgat aacctctctt tgcaaagcaa ctctaacatc
     1981 attagaagac atgcctgcaa taaagttaat accaacaaca tattcctgat gcccagcaat
     2041 aagttctaaa gagcttaaaa taccatctgg aaagttgccg tgtgctacaa taataatttt
     2101 tatcatattt tctcctcttg agtttaccgg tttactttta taatcatatt atagcgcttt
     2161 catttttagg tgtcaacatc ttttttatat tttttgagca gtttacttga ttttttataa
     2221 tgatgtcttt tttatcttta agaatcataa cttagataaa gctttgttat atatgttgcc
     2281 aagttttata ggagaagaac tctatagttg acaaaatcgg tttactttgt tactattcat
     2341 gtaaacaggt ttacttcctg aattttttta cggaggtcat attatgactg aacaattctt
     2401 aaaagacaac ttttcactag aaggtaaagt cgccttaata acaggcgctt cttacggcat
     2461 tggtttttca atagcaactg cttttgctag agctggagca actattgtct tcaatgatat
     2521 taagcaggaa ctcgttgaca aaggcatctc cgcctataaa aaacttggca ttaaggccca
     2581 tggctatgtt tgcgatgtta cagatgaaga tggaatcaac gaaatggtga acaaaatttc
     2641 tcaagatgtt ggggtaattg atattctagt taacaacgca ggtattataa aacgcacccc
     2701 tatgctagag atgagcgctg cagattttcg acaagttatt gatattgatt tgaatgctcc
     2761 ctttatcgta tcaaaagcgg tattacctgg aatgatccaa aaaggacatg gtaaaatcat
     2821 caatatttgc tccatgatga gtgagcttgg acgcgaaaca gttgctgcct atgctgctgc
     2881 caaaggggga cttaaaatgt taaccaagaa cattgcttct gaatacggtt ctgctaacat
     2941 ccaatgtaac ggtatcgggc ccggctatat tgccacacca caaacagctc cccttcgtga
     3001 acgtcaagat gatggttcac gtcatccatt cgaccaattt atcatcgcta aaacacctgc
     3061 cgctcgctgg ggtgaggcag aagatttagg tgcaccagct atcttccttg ctagcgatgc
     3121 atcaaacttt atcaatggtc acatactcta tgtagatggc ggtattttag cttacatcgg
     3181 taaacaacct caataataaa ggagtaatat aaatgaaaat tgcattaatc aacgaaaata
     3241 gccaagcgag taaaaatact cttatctaca aagaattgaa agcggtttcc aaaaaaaaag
     3301 gatttgatgt tttcaactac gggatgtatg gcaaagaaga agaaagccag ctaacttatg
     3361 tacaaaatgg tttactgact gctattttac ttaatagcgg tgctgcagat tttgttatta
     3421 caggctgtgg gacaggaatt ggagcgatgc ttgcttgtaa tagctttcct ggcgtagtct
     3481 gtggattcgc tgctgattct gtcgacgctt acttattctc tcaagtaaac ggaggaaatg
     3541 ctctttccct tccttttgct aaaggatttg gttggggagc tgaattgaat ctgaggtatt
     3601 tatttgaacg tcttttcgaa gatgaaaaag gagggggata ccctaaagag agggctgtac
     3661 ctgagcaacg caatgcacgt atccttagcg aagtaaaaca gattacttac agagatttac
     3721 tctctgtttt aaaagaaatt gatcaagatt tcttaaagga aactatttct ggggagcatt
     3781 tccaagaata tttcttcgct aattgccaaa atcaaaatat agcagactac ttgaaatctg
     3841 tcttagacta aagcgtctgt tttagaaagg acttttatgt ctaagattct ttttttcggt
     3901 gaacctctca ttcgcatcac tcccaaagaa aatgactatt ttgcagatag tatctcaact
     3961 aaactattct atggtggctc tgaagttaat atcgcaagag ctctacaagg ctttgggcaa
     4021 gatactaaac tcctctcagc cttacccaat aatcccatag gtaatagttt tcttcaattt
     4081 ttaaaagcac agggtattga tactcattct attcagtggg ttggagaacg tgttgggtta
     4141 tatttcttag aaaattcctt tgcttgcaga aaaggtgagg tggtctacga ccgcgattat
     4201 agtagtttac atgactttag gataaaccaa attgatttcg atcaattatt tgaaggagtt
     4261 tctctctttc attttagtgg tattacacta tcattggatg agtctatcca agagataaca
     4321 ctgcttcttt taaaagaagc taaaaaaaga gagataacaa tctccttaga tctcaatttt
     4381 cgcagtaaac taatttctcc taaaaatgcc aaaatccttt tttctcagtt cgctaccttt
     4441 gcggatattt gttttggtat tgaacctctt atggttgact cacaggacac taccttcttc
     4501 aatcgcgatg aagctacaat agaggacatc aaagaacgaa tgatttctct tataaatcac
     4561 tttgactttc aagtcatttt ccatactaaa cgtctacagg atgaatgggg acgaaaccac
     4621 taccaagctt acatagcaaa tcgtaaacaa gagtttgtca cttcaaaaga aatcaccaca
     4681 gctgtaaacc agagaattgg aagcggagat gcttttgtgg cgggagcgct atatcaacta
     4741 cttcagcact cagacagcaa gacagtcatt gatttcgctg tcgctagtgc atctttaaaa
     4801 tgtgctttgg aaggggacaa tatgtttgag accgttaccg cagttaacaa agtactaaat
     4861 caatcgaaag acattattcg ttagaaggga gaattatgat aaaatcaaag attttgttgc
     4921 aattaatgca aggaaaaata gttgctgtca ttcgtggaaa tagccaagaa gaagcttttc
     4981 aagctgctca agcatgcata aaaggtggta tttctgctat cgaaatcgcc tatactaata
     5041 gcaaagctag ccaagtcatt gaacaactag tcacacaata tactaaccaa gaacaagttg
     5101 tcgttggggc aggaactgtt ctagacgcag aaactgcccg aatggctatt ttagcaggag
     5161 caaagtttat tgtatctccc gcatttaatc tacagactgc taaattatgt aatcgttatg
     5221 ctattcctta tttaccaggt tgcatgacct tatcagagat aacaacagca cttgaagctg
     5281 gttgtgaaat aattaaaata ttcccaggag gtactctagg aacatctttc atctcatcac
     5341 taaaagcacc tttacctcaa gtacaaatta tggtgactgg tggggtaaat ctcacaaatg
     5401 ccaaagactg gtttctatct ggagtaacag ccattggtat tggtggagag ttcaataaac
     5461 tagcagcttt gggggaattt gataaaatca cagaaatggc taaacaatac aaaatacagc
     5521 taaatctgaa ttaacacaat aaaaagacaa ggcaccccca cacctcaatc atgagacaga
     5581 agaaaagaac ttttataaag ccatttttct gtagtaccgt ttatcttaat cagactcatt
     5641 atctcgattt tcttatcaga ccgagataat ttttagctaa tcttcctgta gacataaccg
     5701 ttctacttcc gacaactgat ccatgtcttc accaaagata tactacttgc ccgcagattg
     5761 atataagtag tgaagctcat cattctcata tcatttcagc aaataataga tttgatatca
     5821 tttttatatc caaaaactta gcaatgacct atcaggctta cccgcttttc ctatctcaac
     5881 acaggctaac ttagctccta tcaaatacat tttttaaaca taattaaaaa catttctgat
     5941 tatagtttat tagatttcaa cagaagtatt tttcctgcgt ctcagctttt gggtgtaccg
     6001 ttattttgaa gttttgatag ttgagtgata tcattaatag tcatttttta gacattctaa
     6061 acctcctttc taatttgacc g
//
LOCUS       DATRQA010000114         5997 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.114, whole genome shotgun sequence.
ACCESSION   DATRQA010000114 DATRQA010000000
VERSION     DATRQA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.114"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            182..1840
                     /locus_tag="VBD27_001622"
     CDS             182..1840
                     /locus_tag="VBD27_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7025621.1"
                     /translation="MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAI
                     PTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDKDGKTRPDLATKVDVSKDGLTYTATL
                     RKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQEKDLNK
                     LGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGP
                     YTVEGWNGSNGTFTLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANIS
                     NTSAIYQANKNNKDVTDVLEATTAYMEYNTTGSVKGLDNVKIRRALNLATNRKGVVQA
                     AVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAESGLTKLK
                     LTITADADAPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSLW
                     GGDYPEGSTFYGLFKSDSQNNDGKFANKDYDAAYNKAISEDAMKPAESAKDYKEAEKI
                     LFEQGAYNPLYFRSGKGLQNPKLKGVIRNTTGLSIDFTHAYKNN"
     gene            1955..2869
                     /locus_tag="VBD27_001623"
     CDS             1955..2869
                     /locus_tag="VBD27_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025622.1"
                     /translation="MIKYILKRVAILLVTLWVVITLSFFLMQILPGTPYNNPKLTEEM
                     ITLLNKQYGLDKPVWQQYLTYLWNVLHGDFGTSYQSVNQPVSRMISLRLGVSVHLGVQ
                     ALVFGVLGGILVGAISARHKNDKVDGILSVIATLGISMPSFIIGILLLDYFGFKWNLL
                     PLSGWGTFSQTILPSLALGLPTLASVSRFFRSEMIETLNSDYVQLARSKGMTIRQVTR
                     KHAYRNSMIPILTLIGPLAAGLLTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTT
                     IVYAVMLMVAILITDVVISIVDPRVRLQ"
     gene            2879..3910
                     /locus_tag="VBD27_001624"
     CDS             2879..3910
                     /locus_tag="VBD27_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025623.1"
                     /translation="MADKNRTFKLVGAGSSSTQEKIEKPALSFMQDAWRRLKKNKLAV
                     VSLYLLALLLTFSLASNLFVTQKDANGFDSKKVTTYRNLPPKLSSNLPFWNGSIKYAG
                     NTESTDAYKSQNVPEKVKYALGTDSLGRSVAKRIIVGIRISLLVAIAATFIDLIIGVT
                     YGLVSGFAGGRLDTLMQRVVEVISSIPNLVIVTMLGLVLGNGITAIIISIAFTGWTSM
                     SRQVRNLTLSYREREFVLAARSLGESPIKIAFKHILPNISGIIIVQIMMTIPSAIMYE
                     AVLSAINLGVKPPTASLGSLISDAQENLQYYPYQVILPALALVMISLAFILLGDGLRD
                     AFDPKSSDE"
     gene            3923..4972
                     /locus_tag="VBD27_001625"
     CDS             3923..4972
                     /locus_tag="VBD27_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025624.1"
                     /translation="MEKETILSVNNLHVDFHTYAGEVKAIRDVNFELKKGETLAIVGE
                     SGSGKSVTTRTLIGLNAKNSEISGNVQFKGRNLVELSEEEWTKVRGNEISMIFQDPMT
                     SLDPTMKIGMQIAEPMMIHQKISKKDALKLALELMKDVGIPNAEEHINDYPHQWSGGM
                     RQRAVIAIALAADPEILIADEPTTALDVTIQAQILNLMKKIQAERDSSIVFITHDLGV
                     VAGMADRVAVMYAGKIVEFGTVDEVFYNPQHPYTWGLLNSMPTTETESGSLESIPGTP
                     PDLLNPPKGDAFAARNEFALDIDHEEEPPYFKVSETHFAATWLLDERSPKVLPPLPIQ
                     KRWEKWNEIEGRKAQ"
     gene            4969..5901
                     /locus_tag="VBD27_001626"
     CDS             4969..5901
                     /locus_tag="VBD27_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7025625.1"
                     /translation="MTENRKKLVEVKNVSLTFNKGKANEVRAIDNVSFDIYEGEVFGL
                     VGESGSGKTTVGRSILKLYDISDGEITFNGEVISHLKGKALHSFRKDAQMIFQDPQAS
                     LNGRMKIRDIVAEGLDIHKLAKSKSDRDSKVQALLDLVGLNKDHLTRYPHEFSGGQRQ
                     RIGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQREQGLTYLFIAHDLSMVK
                     YISDRIGVMHWGKLLEVGTSDDVYNKPIHPYTKSLLSAIPEPDPESERQRVHQPYNPA
                     IEQDGQERQMHEITPGHFVLSTPQEAEEYKKQIL"
BASE COUNT         1962 a         1001 c         1183 g         1851 t
ORIGIN      
        1 atactaggaa ttattatttt cagataattg aatgaagaaa acgtttgtta taaaaatgta
       61 aaaaaatgta aaaaaatgaa agttaaaaaa ttatctgaaa attttgcgtt tttgtctttt
      121 cttaagaatt ttttgtggta taatgttagt cactagataa atgaattagg agaacaaagc
      181 tatggcaaaa cagaaaaata actggcgccg tgttggagtt ggtgtcctta cacttgcttc
      241 agttgcgact cttgctgcat gtggaagtaa atcagcttcc caggattcta atggagcgat
      301 taattgggct attccaacag aaatcaatac actagattta tctaaagtta cagacactta
      361 ctcaaatcta gctattggta actctagtag taatttcctt cgcttagata aagatggaaa
      421 gacaagacca gacttggcta ctaaagttga tgtttcaaaa gatggcttaa cttatacagc
      481 tacattacgt aaaggcttga agtggtcaga tggcagtaaa cttactgcaa aggattttgt
      541 ttattcatgg caacgtttag ttgatcctaa aacagcttca caatatgctt accttgctgt
      601 tgaagggcat gtgcttaatg ccgataaaat caacgaagga caagagaaag acttgaataa
      661 gctaggtgtt aaggcagaag gcgatgacaa agttgttatt actttatcta gtccgtctcc
      721 gcaattcatc tactaccttg cattcactaa cttcatgcca caaaaacaag aagttgttga
      781 aaaatatgga aaagattacg caactacttc aaaaaataca gtttactcag gaccatatac
      841 tgttgaaggt tggaatggtt cgaatggtac tttcacgctg aagaaaaaca aaaattattg
      901 ggacgctaaa aatgtaaaaa caaaagaagt tcgcatccag actgttaaaa aaccagatac
      961 cgccgttcaa atgtataaac gtggtgagtt agatgcagct aatatctcaa atacttctgc
     1021 tatttatcaa gctaataaaa ataataaaga tgtcacagat gttctagaag cgaccactgc
     1081 ctatatggaa tataatacta ctggttctgt gaaagggctt gataatgtta agattcgtcg
     1141 cgccttaaac ttagcaacta accgtaaagg agttgttcaa gcagccgttg atacaggctc
     1201 aaaaccggca attgcttttg cacctactgg tttagccaaa acaccagatg gaactgattt
     1261 ggcaaaatat gttgccccag gttatgaata taataaaact gaagcagcaa aactctttaa
     1321 agaaggtttg gctgaatcag gcttgactaa gctaaaatta acaattacag cagatgctga
     1381 tgctcctgct gccaaaaact ctgttgacta tatcaagtct acttgggaag ctgctcttcc
     1441 aggacttact gttgaagaaa aatttgtaac ctttaaacaa cgcttagaag acagtagaaa
     1501 acaaaacttt gacattgtag tttctctttg gggtggggat tatccagaag gatcaacttt
     1561 ctacggcctc tttaagtcgg attcacaaaa taacgatgga aaatttgcta acaaggacta
     1621 tgatgctgct tataacaagg caatttctga agatgcgatg aaaccagcag aatcagcgaa
     1681 ggactataaa gaagcagaga aaattttatt tgagcaaggt gcttataacc cactctactt
     1741 ccgtagtggt aaaggtttac aaaatccaaa attaaaaggt gttattcgta atactacagg
     1801 tttgtcaata gactttacac atgcttataa aaataattaa attactttac actaaacctt
     1861 tgcaagaatt ggtaattgcc agttcttgct tttggttaag taaagaaagt gaaagagttt
     1921 gctattttta gcaattctaa gaaaaggaaa tgatatgatt aaatatattt taaagcgtgt
     1981 tgctattttg ttagtaacgc tatgggttgt cataacccta tcgttctttc tgatgcagat
     2041 ccttccagga acaccctaca ataatccaaa actcactgaa gaaatgatca cattgcttaa
     2101 taaacagtat ggtttagaca agcctgtatg gcaacaatat ttgacttacc tttggaatgt
     2161 acttcatggt gattttggaa cgagttatca atcagtgaat cagccagtat cacgtatgat
     2221 tagcttgaga ttaggagttt cagtacatct aggtgttcag gctctcgtat ttggtgtttt
     2281 aggcggtatt ttggtaggag ctatttcggc acgtcataag aatgataaag tcgatggtat
     2341 cttaagtgtt attgcaacgc tagggatttc tatgccttca tttatcattg gtatcttatt
     2401 attagactat tttggattta agtggaactt gttgccttta tcaggttggg ggactttctc
     2461 tcaaacaatt ttaccttctt tagctctagg actccctaca ttagcatctg tttcacgctt
     2521 cttccgtagt gaaatgattg aaactttaaa ttctgattat gtgcaactag ctcgttcaaa
     2581 aggtatgaca attcgtcaag taactcgaaa acatgcttac cgtaattcta tgattccaat
     2641 tttaactcta attggccctt tggcggcggg tctgttaact ggttctgcac taattgagca
     2701 gattttctcg attccaggaa ttgggcagca gtttgttact tcgattccaa caaaagacta
     2761 tcctgttatt atggggacaa ccattgttta tgcagtaatg ttgatggttg ctatcttgat
     2821 aacagacgtt gttattagta tcgtagaccc acgtgttcgc ttgcagtaag gagatgttat
     2881 ggcagataaa aacagaacat ttaaacttgt aggtgcagga tcttctagca cacaagaaaa
     2941 aattgaaaag cctgctcttt cgtttatgca agatgcgtgg cgtcgcttga aaaaaaacaa
     3001 attagcagta gtttcactct atttattagc tcttttactt actttttcgt tagcctcaaa
     3061 tttatttgta actcagaagg atgctaatgg gtttgattcg aaaaaagtaa cgacatatcg
     3121 caacttacca cctaaattga gttcaaacct tcctttttgg aatggtagca ttaagtacgc
     3181 tggaaataca gaatctacag acgcttataa gtctcaaaat gtccctgaaa aagttaagta
     3241 tgctttaggt acagattctc tagggagaag tgttgctaag cgcatcatcg taggtattcg
     3301 aatttcctta cttgtagcta ttgcagctac attcattgat ttaattatcg gtgttacata
     3361 cggtcttgtt tcaggttttg ctggaggacg ccttgataca ttaatgcaac gtgtcgtaga
     3421 ggtaatttca tcaattccaa acttagttat tgttacaatg ttaggtttgg tccttggtaa
     3481 cggtattacg gctattatta tctctattgc ttttaccgga tggacttcta tgtctcgtca
     3541 ggtaaggaat ttaaccctat catatcgaga aagagagttt gtgcttgctg cacgttcact
     3601 tggggaaagt ccaattaaaa tagccttcaa acatatttta cctaacattt ctggtattat
     3661 cattgtacaa attatgatga caatcccaag tgctattatg tatgaagcgg tactttcagc
     3721 aatcaattta ggtgttaaac caccgacagc ttcattagga tcattgattt cagatgcaca
     3781 agaaaattta caatattatc catatcaagt tatcttgcca gctttagcat tggtaatgat
     3841 ttcattggca tttattttgc ttggtgatgg gttgcgagat gcttttgatc caaaatctag
     3901 cgatgaataa ggaggtataa tcatggaaaa agaaactatt ttaagtgtta ataatcttca
     3961 tgttgacttc cacacatatg ctggagaagt aaaagcaatt cgtgatgtca actttgaatt
     4021 aaaaaaaggt gagactcttg caatcgttgg tgaatctggt tcaggaaaat ctgtaactac
     4081 cagaacttta attggtttaa atgctaaaaa ttcagagata tcagggaatg ttcaatttaa
     4141 ggggcgtaac cttgttgaac tatcagaaga agagtggact aaggtacgcg ggaatgagat
     4201 ttctatgatt ttccaagacc ctatgactag tttggatcca acgatgaaaa ttggtatgca
     4261 aatagcggag ccaatgatga ttcatcaaaa aatttcaaaa aaggatgctt taaaattagc
     4321 tcttgagcta atgaaagatg taggtatccc aaatgctgaa gagcatatta acgattaccc
     4381 acatcagtgg tctggaggaa tgcgtcaacg tgcggttata gcgattgctt tagctgctga
     4441 tcctgaaatt cttattgctg atgaaccaac gactgcttta gatgtaacaa ttcaagcaca
     4501 aattttaaac ttaatgaaaa aaattcaagc agaacgagac tcctccattg ttttcataac
     4561 tcatgactta ggggtcgttg cagggatggc agaccgtgta gcagttatgt atgcagggaa
     4621 aatcgttgaa tttggaactg ttgatgaagt cttttataat ccacaacatc catatacttg
     4681 gggattattg aattcaatgc cgacaaccga aacagaatca ggtagtttag agtcaattcc
     4741 gggaacacca ccagatttgt tgaacccacc taaaggagat gcatttgcag ctcgtaatga
     4801 atttgcatta gatattgatc atgaagaaga accaccgtac ttcaaagttt cagaaacaca
     4861 tttcgctgct acatggcttt tagatgaaag atcacctaag gtgcttcctc cattaccgat
     4921 ccaaaaacgt tgggaaaaat ggaacgagat tgaagggagg aaagcccaat gactgaaaat
     4981 cgaaaaaaat tagttgaagt caaaaatgtt tctttgacct tcaataaagg aaaagctaat
     5041 gaagtgagag caattgataa tgttagtttt gacatttatg aaggtgaagt gtttggatta
     5101 gttggggaat ctggatcagg aaagacaacc gttggacgtt caattttaaa actttatgat
     5161 atttctgatg gcgaaattac ctttaatgga gaagtgatct cacatctgaa aggtaaagca
     5221 ttacatagtt ttcgtaaaga cgctcagatg atttttcagg atcctcaggc tagcttaaac
     5281 ggccgtatga agattcgtga tatcgtagca gaaggtttag acatacataa attagctaaa
     5341 tcaaaatcag atcgtgatag taaagttcag gccctattag atcttgtagg gttaaataaa
     5401 gatcatttaa cacgttatcc gcatgaattt tcaggtggac aacgacaacg tatcgggatt
     5461 gcacgtgctt tagcagtaga gcctaaattt atcattgctg atgaaccaat atctgcctta
     5521 gatgtttcaa tccaagcaca agttgttaat ttaatgcaaa aattgcaaag agaacaagga
     5581 ttgacttatt tgtttatcgc acatgatttg tctatggtaa aatacatttc agatcgtatc
     5641 ggtgttatgc attgggggaa actgttagaa gttggaacat ctgatgatgt ttataacaaa
     5701 ccaatccacc cttatacgaa gagtctatta tcagctattc cagaaccaga tccggagagt
     5761 gaacgtcaac gtgttcacca gccgtataat ccagctattg agcaagacgg acaagaacgt
     5821 caaatgcacg agattacacc tggtcatttt gttttatcta caccccaaga agctgaagaa
     5881 tataaaaaac aaatattata aaagatggct agcccatctt ttattttttt actaataaaa
     5941 taaatttagg tatggaattt taaactctat gttattggtg ttagccaaca ggaaact
//
LOCUS       DATRQA010000115         5824 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.115, whole genome shotgun sequence.
ACCESSION   DATRQA010000115 DATRQA010000000
VERSION     DATRQA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.115"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            11..571
                     /locus_tag="VBD27_001627"
     CDS             11..571
                     /locus_tag="VBD27_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO7025626.1"
                     /translation="MNYHDYIWDLGGTLLDNYESSTRAFVETLKEFGYQADHDSVYQK
                     LKESTADAIAYFIPEEADFLKEYKANEAKVLETPILFQGAKELLAKIQRQGSRNFLVS
                     HRDNQVIVILEKTEIIDYFTEVVTADNGFSRKPSPESMLYLKEKYQIDNCLVIGDRDI
                     DKQAGESAGFDTLLVDGSKSLMEIIE"
     gene            572..2524
                     /gene="gyrB"
                     /locus_tag="VBD27_001628"
     CDS             572..2524
                     /gene="gyrB"
                     /locus_tag="VBD27_001628"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO7025627.1"
                     /translation="MTEETKNMEQRAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFAGHIKVYIEPDNSITVVDDGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKNGKVHYQEYQRGVVV
                     NDLEIIGDTDLSGTTVHFTPDPEIFTETTVFDFDKLAKRIQELAFLNRGLRISISDKR
                     EGQEVEKEYHYEGGIGSYVEFINENKEVIFENPIYTDGELDGISVEVAMQYTTGYQET
                     VMSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFNRFLLENPQVAKKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNNAEMNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKVRYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKAYIQPGVNQEEE
                     LRQALDTYSSGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVSVDDAAEADKIF
                     DMLMGDRVEPRREFIEANAVYSNLDI"
     gene            2618..4342
                     /gene="ezrA"
                     /locus_tag="VBD27_001629"
     CDS             2618..4342
                     /gene="ezrA"
                     /locus_tag="VBD27_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEO7025628.1"
                     /translation="MSSGIILLLVAIVLLVIIAYVVGVVIRKRNDTLIANLETRKQEL
                     VDLPVQEEIEQVKLLHLIGQSQSTFREWNQKWTDLSTNSFKDIDFHLVEAENLNDSFN
                     FVRAKHEIDNVDSQLTIIEEDIVSIREALEVLKEQEEKNSARVTHALDLYETLQKSIS
                     EKEDNYGTTMPEIEKQLKNIEAEFSHFVTLNSTGDPIEASEVLNKAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKDIEQRFQEVREAIRSNSDGLV
                     SLDLDRARDENEHIQEKIDKLYDIFEREIAAYKVAHKDSKIIPQFLAHAKSNNEQLGH
                     EIKRLSAKYILNENESLSLRSFTNDLEEIETKVLPSVENFGQEASPYTHLQILFERTL
                     KTLTTVEENQMEVFEAVKTIESVETRARQNMDKYVNKLHMIKRFMEKRNLPGIPQDFL
                     STFFTTSSQIEALINELSRGRIDIEAVSRLNDVTTNAIANLEQATYLVVQDATLTEQL
                     LQYSNRYRSFEQNVQKSFEQALYLFEVEHNYKASFDEISYALETVEPGVTDRFVTSYE
                     KTQERIRF"
     gene            4436..5077
                     /gene="serB"
                     /locus_tag="VBD27_001630"
     CDS             4436..5077
                     /gene="serB"
                     /locus_tag="VBD27_001630"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEO7025629.1"
                     /translation="MIKGLLVMDVDSTLIMEEAIDLLAIEAGVGKQVAALTDAAMRGE
                     LDFEEALKKRVALLKGLPVTILTDILSSIHFTPGAYELIKECHKRQMKVGLVSGGFHE
                     TIDILAKQLQVDYVKANRLGVKGGFLTGEVEGEIVTKEVKKIKLKEWASENHLDLSQT
                     IAMGDGANDLPMIKSAGVGIAFCAKPIVREEAAYQINEKNLLKVFDIIDANEN"
     gene            complement(5098..5583)
                     /locus_tag="VBD27_001631"
     CDS             complement(5098..5583)
                     /locus_tag="VBD27_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO7025630.1"
                     /translation="MEIWDAYDQYGFITGLTLNRDQNIPQGLFHLVVDVILFHEDGDV
                     LMMKRHPKKKAFPAYFEATAGGSALKGENAKQAILRELKEETGIVPQCLTFLNREWFS
                     ERSYFVDHFIAKYNGAKDIITLQEGETVDYIWLKPEYIDLFLSKNKLIPSQIKLLKSL
                     I"
     gene            complement(5596..>5824)
                     /locus_tag="VBD27_001632"
     CDS             complement(5596..>5824)
                     /locus_tag="VBD27_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1694 domain-containing protein"
                     /protein_id="HEO7025631.1"
                     /translation="DVQMFYMKLANRLSLAATIVNEANANSPFGIIIHSDKAENVEWN
                     DFETQFPDLFNSPKKEESPKKSLWQHFFSQK"
BASE COUNT         1963 a          892 c         1196 g         1773 t
ORIGIN      
        1 aataaagggt atgaattatc atgattatat ttgggatttg ggtggtacac tacttgataa
       61 ttacgaaagt tccacacgtg cttttgtaga gactttgaaa gaatttggat atcaagcaga
      121 ccacgatagt gtgtatcaaa aattaaaaga gtcaacagca gatgcaattg catattttat
      181 ccctgaagaa gctgattttt taaaagagta taaggctaat gaagctaagg ttttggaaac
      241 accaattcta ttccaagggg caaaagaact tttagcgaaa attcaaagac aaggtagtcg
      301 taattttttg gtgtctcatc gtgataatca agttatagtt attctggaaa aaacagaaat
      361 tattgactat tttacagaag tagtgactgc tgataacggc ttttcaagga aacctagtcc
      421 cgagagtatg ctttatttga aagaaaaata ccaaattgat aattgtttag taataggcga
      481 tcgtgatata gataagcaag caggagaatc ggctggtttt gataccttat tagtcgatgg
      541 gtcaaaatca ttgatggaga taatagagta aatgacagaa gaaacaaaga atatggaaca
      601 acgtgctcaa gaatatgacg caagtcagat tcaagtatta gaaggtttag aagctgttcg
      661 catgcgtcca ggcatgtata tcggttcgac ttcgaaagaa gggcttcatc accttgtatg
      721 ggaaattgtt gataactcaa ttgatgaagc cttagctgga tttgcaggac atattaaagt
      781 ttacattgaa ccagataact ctattacagt tgttgatgat ggtcgcggta ttccggtaga
      841 tatccaagaa aaaactggaa gaccagctgt agagacagtt tttacagttc tccatgccgg
      901 tggtaaattt ggtggtggcg gatacaaggt ttctggtggc ttacatggtg tagggtcatc
      961 tgttgttaat gcattgtcaa cacaattaga tgtcaaggtt tataaaaatg gcaaggttca
     1021 ttatcaagaa taccaacgcg gtgttgttgt taatgatctt gaaattatcg gtgatactga
     1081 cttatcagga acaacagttc actttacgcc tgacccagaa attttcactg aaaccactgt
     1141 atttgatttt gataaattag ccaaacgtat tcaggaatta gcttttttaa atcgtggact
     1201 aagaatctcc atttcagaca agcgagaagg tcaagaagtt gaaaaagaat atcactatga
     1261 aggtggtata ggttcatacg ttgagtttat caatgagaac aaagaagtga tttttgaaaa
     1321 tcctatttat actgacggtg aattagatgg tatttcagtt gaagtagcaa tgcagtatac
     1381 tactggatat caagagactg ttatgagttt tgcaaataac atccacactc atgaaggagg
     1441 aacgcacgaa caaggatttc gtactgcctt gacacgtgtg attaatgatt acgctaaaaa
     1501 gaataagatt ctcaaagaaa atgaagataa tttaactgga gaagatgttc gtgaaggttt
     1561 aacagcagtt atatctgtta aacatccaaa tccacaattt gaaggacaaa ctaaaacaaa
     1621 acttggaaat tctgaagttg ttaaaattac aaatcgtctc tttagcgaag ctttcaatcg
     1681 attcctatta gaaaatccac aagtagctaa gaagattgtt gaaaaaggaa ttttggcatc
     1741 taaagcccgt attgcagcaa aacgggcgcg tgaagtaaca cgtaaaaagt caggcttaga
     1801 gatttcaaac ttacctggga aactggctga ttgctcatct aataatgctg agatgaatga
     1861 actttttata gtggagggag attctgcggg agggtctgca aaatcaggac gtaatcgtga
     1921 gtttcaagca attttaccga ttaggggtaa gattctaaac gttgaaaaag ctacaatgga
     1981 taaaatttta gcaaacgaag aaatccgaag tctcttcaca gctatgggaa ctggatttgg
     2041 tgcagatttt gatgtatcaa aagtcagata tcaaaaatta gtcattatga ctgatgccga
     2101 tgttgatgga gctcacatca gaactttact tttaactctg atttatcgtt ttatgcgacc
     2161 tgttttggaa gcaggatatg tttatatcgc tcaaccacca atctatggtg tcaaagtagg
     2221 aagtgagatt aaagcttata ttcagccagg ggtcaatcaa gaagaggaat tacgtcaagc
     2281 tctagatacc tatagttcag gacgttcaaa acctactgta caacgttaca aaggtctagg
     2341 tgagatggat gaccatcaac tttgggaaac aacaatggat cctgaaaacc gcttgatggc
     2401 acgtgtctca gttgatgatg cagcagaagc agataagatt ttcgatatgc taatgggaga
     2461 tcgagtagaa ccacgacgtg aattcattga agctaatgct gtttatagta acttagatat
     2521 ctagtccaaa gatagctttg aaaaacagtt caagtatgct ataatcttaa cgttatagta
     2581 gatttttaga aaattagacc attataagga gagttgaatg tcgagcggaa tcatattgct
     2641 gcttgtggct atagtcctgc ttgtgattat tgcctacgta gtcggcgtcg taataagaaa
     2701 acgtaatgat acattaattg caaacttaga aactagaaaa caagagttag ttgaccttcc
     2761 tgtgcaagag gagattgagc aagtcaaatt gcttcatttg attggccaaa gtcaatcaac
     2821 ttttagagaa tggaaccaaa aatggactga tttatcaact aattcattta aagatattga
     2881 ttttcattta gttgaagctg aaaatcttaa tgattcattt aattttgtgc gtgctaagca
     2941 cgagattgat aatgttgata gtcagctaac tattatagaa gaagatattg tttcgattcg
     3001 tgaagcttta gaagttttaa aagagcaaga agagaaaaat agtgcccgtg taacacatgc
     3061 tttggaccta tacgaaacac ttcaaaaatc aatttctgaa aaagaagata attacgggac
     3121 aaccatgcca gaaattgaaa agcagttgaa aaatatcgag gctgaatttt cacattttgt
     3181 gaccttaaac tcaactggtg acccgattga agcttctgaa gttttgaata aagcagagga
     3241 gcatacgatt gctttagggc aaattacaga acaaattcca gctattgttg ctaaattaga
     3301 agacgatttt ccagatcagt tagatgattt ggagacagga tatcgtcgtc ttttagagga
     3361 aaactaccat ttccctgaaa aggatattga acaacgtttt caagaagttc gtgaggctat
     3421 tcgttcgaat tcagatggtt tagttagttt ggatctagat cgtgctcgcg atgaaaatga
     3481 acatattcaa gaaaaaattg ataaacttta tgatattttt gaacgtgaga ttgctgctta
     3541 taaagtagct cataaggata gtaaaattat tcctcaattc cttgctcatg caaaatctaa
     3601 caatgaacaa ctggggcatg aaatcaaacg attatctgct aaatatatct taaatgaaaa
     3661 tgaaagctta agtttacgtt ccttcacaaa tgatttggaa gaaattgaaa caaaggtttt
     3721 accttctgta gaaaattttg gtcaagaagc ttcaccttat actcatctac aaattttatt
     3781 tgagcgtacg ttgaaaacat tgactactgt tgaagaaaat caaatggaag tttttgaagc
     3841 cgttaaaact attgaaagcg ttgagacacg agcacgccaa aatatggata agtatgttaa
     3901 caagctccat atgattaaac gtttcatgga aaaacgcaat cttccaggaa tcccgcaaga
     3961 cttcttaagc actttcttta caacaagttc tcaaattgag gcacttatta atgaactcag
     4021 tcgcggtcgt attgatattg aggctgtttc acgtttaaat gatgtgacta caaatgctat
     4081 cgctaattta gagcaagcta catatctagt tgttcaagat gcgactctca cagagcagtt
     4141 actacagtat tcaaaccgtt atcgtagttt tgaacaaaat gtgcaaaaga gctttgaaca
     4201 agctttgtac ttatttgaag ttgaacataa ctataaagca tctttcgatg aaatttcata
     4261 cgcacttgag actgtggaac caggtgtaac tgaccgtttt gtgacgtctt atgaaaagac
     4321 acaagaacgt attcgtttct aaaaaagaag agttagtact cttctttttt taattgctat
     4381 ctatgaaagg tgctaatttt aaggtacaat agtaaaaaag ttttggaggt tttaaatgat
     4441 taaaggtttg ttagtcatgg acgttgattc aaccttaata atggaagaag ctattgattt
     4501 attagctatt gaagcagggg tggggaaaca ggttgctgca ctgactgacg ctgcaatgcg
     4561 tggcgagtta gattttgaag aagctttgaa gaaaagagta gcgctactta aaggtttgcc
     4621 tgttactatt ttgacagata tattatcctc aatacatttt actccagggg cttatgaatt
     4681 gattaaagag tgtcacaagc gtcagatgaa agttggtctt gtatcaggag gttttcacga
     4741 gacgattgat atcttagcta agcaattaca agttgattat gtcaaagcga atcgtcttgg
     4801 tgtcaaaggt ggttttctca ctggcgaagt tgagggagag attgtcacta aagaagtgaa
     4861 aaaaataaag ttaaaagagt gggcttctga aaatcactta gacctttcac aaaccatcgc
     4921 aatgggagat ggtgccaatg atttaccaat gatcaaatcc gctggggtag gaattgcttt
     4981 ttgtgcaaaa ccaattgtca gagaagaagc tgcgtatcag attaatgaaa aaaatttgct
     5041 taaggtattt gatattattg atgcaaatga aaactaggcc tttattaagc ctagttttta
     5101 gattaaggat tttaagagtt ttatttgaga gggtattaat ttgtttttag ataaaaataa
     5161 atcaatatat tctggtttta accagatata gtcaacggtc tctccttctt gcaatgtaat
     5221 tatatctttt gcaccattgt attttgcgat aaaatggtct acaaagtaag aacgctcaga
     5281 gaaccactct ctattaagaa aggttagaca ttgaggaacg ataccagttt cttctttgag
     5341 ttcccttaga atagcttgct tagcattttc tcctttaagg gcagatccgc cagcggtggc
     5401 ttcgaaataa gcaggaaaag ctttcttttt agggtgccgt ttcatcatca atacgtcacc
     5461 atcctcatga aaaaggataa catcaacaac taagtggaat aagccttgtg ggatattttg
     5521 atccctatta agtgtaagac ctgtaatgaa cccatattgg tcataagcat cccaaatttc
     5581 cattagacct ccttgttatt tttgactaaa gaaatgttgc catagacttt ttttaggaga
     5641 ctcctctttt tttggtgaat tgaataaatc agggaattgt gtctcaaaat cgttccattc
     5701 aacgttttca gccttatcac tatgtataat aataccaaat ggagaattgg catttgcttc
     5761 attaacaatc gtagcagcta gtgatagacg gttagctaac ttcatgtaga acatttgaac
     5821 atca
//
LOCUS       DATRQA010000116         5792 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.116, whole genome shotgun sequence.
ACCESSION   DATRQA010000116 DATRQA010000000
VERSION     DATRQA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.116"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..281
                     /gene="essA"
                     /locus_tag="VBD27_001633"
     CDS             <1..281
                     /gene="essA"
                     /locus_tag="VBD27_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEO7025632.1"
                     /translation="DKNRQSKVSLQKKQSKQLFQYSNKGQSDRKKRIKQLFSEESNQF
                     KFVTRTEEATVSADIGHMIMYLVLISLVLILASIMTFKIKDKKDEYTY"
     gene            265..507
                     /locus_tag="VBD27_001634"
     CDS             265..507
                     /locus_tag="VBD27_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEO7025633.1"
                     /translation="MNTHINITLDMGETSKDLRIPLKISVKQLVMELDSIFSRTERRT
                     KYQLRVINKGILLGENDILAHHPVTTGDRIRIEEFY"
     gene            507..1781
                     /gene="essB"
                     /locus_tag="VBD27_001635"
     CDS             507..1781
                     /gene="essB"
                     /locus_tag="VBD27_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HEO7025634.1"
                     /translation="METDSFVFEEQDFQFEKGPDFWQLSLKRSKVGTQNLQQLQLLEI
                     HHSLLMPMTTAINADTIQFQFQTEAHALSFESFKKETLSEKLRLALNVLDLDKALSLP
                     VNFILHPSNLFLTKNATAKIAYRSLPGIMTPEKFGPEEFLYQFKCFVFALLTQHDYIE
                     LYNGAISVIEVSDFLKSIYHAETIQAVRDIITTDYEQQVEVETHTLAKVSKAKYKLYK
                     YISVWLGALSTILLIPLVYLVFIHNPFKEKMLAADTSFIKVDYNQVINRLEHVKVSKL
                     PYTQKYELAYSYINGMSFSEEQREVILNNVTLKTDELYLDYWINIGRGLDDDAIDAAK
                     RLDDSDLVIYAIVQKMDQVRKDNSLSGKDREQKLSELQTDYDKYWKDRKTALTDEESK
                     SKNSNNHSTNSNKESSESSSTTASTSSKTKSR"
     gene            1788..5792
                     /gene="essC"
                     /locus_tag="VBD27_001636"
                     /pseudo
     CDS             1788..5792
                     /gene="essC"
                     /locus_tag="VBD27_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960148.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
BASE COUNT         1946 a         1045 c         1057 g         1744 t
ORIGIN      
        1 tggataaaaa ccgtcaatct aaagtctcac tccagaaaaa gcagtctaag caactgtttc
       61 aatattcaaa taaaggacaa tctgatagga aaaaacgtat caaacaatta ttttctgaag
      121 aaagtaatca atttaaattt gttacaagaa cagaagaagc gactgtttcg gctgatattg
      181 gtcatatgat tatgtacctg gttctcatta gtttggtact aatattagct tctataatga
      241 cttttaaaat aaaggacaaa aaggatgaat acacatatta acattactct agatatgggg
      301 gaaacctcaa aagatttacg tatccctttg aaaattagcg ttaagcaatt agtaatggaa
      361 ttagatagta tttttagccg tactgaaagg cgtactaaat atcaactaag agttataaat
      421 aaaggtattt tattgggaga gaacgatata ttagcgcacc atccagtaac aactggtgat
      481 cgtataagga tagaggagtt ttattaatgg aaacagattc gtttgtattt gaagaacaag
      541 actttcaatt tgaaaaagga ccagattttt ggcagttatc tcttaagcgt tcaaaagtag
      601 gaactcaaaa tctgcaacaa ctgcaattgt tagaaattca ccattctttg ttgatgccta
      661 tgacaactgc aattaatgca gataccattc aatttcaatt ccagacagaa gcgcacgctc
      721 tatcttttga aagctttaaa aaagaaactt tatctgaaaa attacgatta gccttgaatg
      781 tcttggattt agataaggct ttgtctttgc cagtaaattt cattttgcat ccctctaatc
      841 tatttttaac aaaaaatgca actgctaaaa tagcttatcg tagtcttcct gggattatga
      901 cacctgagaa gtttggtcca gaggagtttt tatatcaatt caaatgtttt gtctttgcat
      961 tactgacgca acatgactat atagaattgt ataatggtgc tatttctgtt attgaagtat
     1021 cagatttcct aaaaagcatt tatcatgcag aaactattca ggctgttaga gacatcatta
     1081 ctactgatta tgagcagcaa gtagaagtag aaactcatac gttagcaaaa gtatcaaagg
     1141 caaaatataa gctttataaa tacataagtg tctggctagg agctttatct acaatactct
     1201 tgattccgct agtatatctg gtttttattc acaatccatt caaagaaaaa atgttggcag
     1261 cggatacttc atttattaaa gttgattata atcaagtgat taatcgacta gaacatgtaa
     1321 aagtaagtaa gttaccttat acacagaagt acgagttggc ctattcctat attaatggaa
     1381 tgtcattttc tgaagaacag cgtgaagtta ttttaaacaa tgttacgctc aagactgatg
     1441 agctctatct tgattattgg attaatattg gccgtggttt agatgatgat gctattgatg
     1501 ctgccaaacg tttagacgat tctgaccttg ttatttatgc tattgttcag aaaatggatc
     1561 aggttagaaa agacaatagt ttatctggta aagatcgtga acaaaaactt tctgagctac
     1621 aaacagacta tgataaatat tggaaagacc gtaagacagc cttaacagat gaggaatcca
     1681 aatcgaaaaa tagcaataat cattcgacaa attccaacaa agagtcatct gaatcatcaa
     1741 gtactacagc aagtacatct tctaaaacta aaagtaggta ggtggatatg ttagttattt
     1801 gttattatca atcactccgc tacgaattta atcttgaaga ggagaaatcc tttctaatat
     1861 catcaaacgg caagtcgcca ataccagtat cagatttaga aaatgacatc acgctaaaaa
     1921 atatgcaagg tcaattggta tatattgttg accaaaaaga aaaagagtta actaatggtg
     1981 tagagatttc aggaattgtc ttctatttag cgaataatca aaaagaaatc tatactccct
     2041 tagattacga agatatatta atcggtgata aagaagggta tcatgtccgt tttaaagaag
     2101 gagcccctaa tttattactg aaaaaaatag aaagcaattg gcagcttaat ttatttgaag
     2161 gtgatattta tctgaataat catcttcaaa aagtagtgca gcagttacct ttaagtttag
     2221 gtgatgagat tagcttccaa ggaacaattg ttaagctttt cccagatgaa attcaaatct
     2281 gggggggggg actgattatg aaacatcatt aactaagaaa gttatgtcag cttatcaatt
     2341 ttacgaaggc tatcctgatt ttcaccgctc acctcgtatt atttatcgtt ccagtgaaga
     2401 taaaattaca gttaatgcgc ctggtaataa acctaataag tctaaagatg aactgttaaa
     2461 attaattgtt cctcctctag tgatgattgg agtcagtatt cttatttcta tttttcgtcc
     2521 tcgtggtatc tatatcattg caacaatgtc aatggctcta gtgacgatga ttttctctat
     2581 cactggttac tttaaaaatc gtaaacagta taagcaggac ttacaagaac gcattgattc
     2641 ctatcatgac tatttatctg ataagtcaat tgaacttcag aagttagcta aagagcaaaa
     2701 gcgtgggcaa cactatcatt acccaacgat tgaaggttta caggaaatgg ctgatactta
     2761 tcaccatcgt atttatgaaa agacaccact tcattttgac ttcttgtatt atcgcctagg
     2821 tttgggagaa gtaccaacga gctataatat tcattattct caaccagaac gttctgggaa
     2881 aaaagatcct ttagagaacg aaggctataa cctctacttt aacaatcgtt atattaaaaa
     2941 tatgccgatt gttgcgaatt tatcacatgg accagttggc tatataggac cacgaggttt
     3001 agtattggag caactccaat tgatggttaa tcaattagct tttttccatt cttaccatga
     3061 tgttcaattt attactattg ttccagagga agagatggat aagtggtctt ggatgcgttg
     3121 gttgccacat gcaaccttac aagatgtcaa tgttcgtggc tttgtttata atcaaagaag
     3181 ccgtgatcaa gtgctcaaca gtcttaacca aattttaaaa ttacgtcgca cacaacgcga
     3241 agataaatct gccaaagaag ggactttatt ttctccgcat tatgtcgtta ttgtaactga
     3301 tgaaaagtta attttagatc acgtcatcat ggaatttttt acggaagatc ctacagaatt
     3361 gggatgttct ctcatctttg ttcaagatgt tatgagttca ctttcagaaa atatcaaaac
     3421 cattattaat attaaagacc gtaatactgg tcagttagtt atagaagaag gagagcttaa
     3481 agaaacagat tttgaattag atcatttctt agaggattat gacaaggaaa atatttcacg
     3541 tcgcctagct ccgttaaatc acctacaaaa tcttaaatct tcaataccag aagcagttac
     3601 ttttatggaa atgtatcaag cagaagaatt tgaagatttg catgttcaag agcgttggat
     3661 aagccatgct ccgtataaaa gcttagctgt tccacttggc ttacgtggtc aggatgatat
     3721 tgtttatcta aacctacatg aaaaagctca tggtcctcat ggattagttg caggaacaac
     3781 gggatcaggg aagtcagaaa ttatccagtc ctatatcctt tctttagctg ttaatttcca
     3841 tccacacgat gtagctttcc ttctcataga ctacaaaggt gggggaatgg caaatctctt
     3901 taaggacctg ccacacttac ttggcaccat caccaactta gatggtgccc aatcgatgcg
     3961 tgctttggtt tctattaatg ctgaattgaa acgtcgtcaa cgtttatttg ctaaagctga
     4021 tgttaatcat attaaccagt atcagaagaa atacaaactt ggagaagttt cagaaccaat
     4081 gcctcatctc ttcttaatct ccgatgagtt tgccgaattg aaatccaacc aacctgagtt
     4141 tatgaaagag cttgtctcaa cagcgcgaat tggtcgttcc ctagggattc acttaatcct
     4201 agcgacccaa aaaccatctg gcgtggtaga tgaccaaatc tggtcaaact cacgcttcaa
     4261 actagctctt aaagtagcag accgcggcga ctccatggaa atgctccata ccccagatgc
     4321 cgccgagatt acacaagcag gacgtgccta cctccaagtt ggtaataatg aagtctatga
     4381 aatcttccaa tcagcatggt caggagcaga ttaccaacct gaaaaagacg accaagggat
     4441 tgaagaccat accatttatt ccatcaacga ccttggccaa tatgaaatcc taaatgatga
     4501 tctttcaggc ttagaccaag ccgaaaatat taaagaagta cctacggaat tagatgccat
     4561 cgttgaaaac attcaaactt taaccaaaga aatgggcatc tcagacttac ctcaaccgtg
     4621 gctaccgcct ctaagcaatc agattgcagt aaccgatctc cgtaaggaag aatcagtaga
     4681 tctctggagt aaagccccat cttacaaagt tgttctgggc tttatggata taccaagcca
     4741 acaagcacaa gaagtagctt accacgattt tgaagatgat gggcatttgt caatttttgc
     4801 aggtccaagt atggggaaat ccacagccct tcaaactgta acattggact tagcacgtca
     4861 caatagtcca gaatttttaa acctttatct ctttgatttt ggtaccaatg gcctcttacc
     4921 attaagacgt ttgccacatg tagcagattt ctttaccatt gatgatgacg aaaaaatagc
     4981 caagtttatc agcagaatca aaacggaaat gtctgaccgt aaaaaagccc tcagtcgcta
     5041 caatgttgca acagcaaaac tttaccgcca ggtatctggg gaaaccatgc ctcaaatcct
     5101 gattgttatt gacagttatg aagggctacg ggaagctcaa acactgacta acttagaagc
     5161 ttgtttccaa aatatttcgc gtgatgggtc gtcacttggc atttccttag ttatctcagc
     5221 tggtcgtatg gcagctttga ggtcgtcctt aatggcaaac cttaaagaac gtatcgctct
     5281 taaattaaca gatgactctg aatcacggac acttgtcggt cgtcaccaac acatcatgga
     5341 agatattcca ggacgcggat taatcaaacg tgatgacatt gaagtgttac aagtggctct
     5401 cccaacagaa ggcactgaaa cctttgacat catcaataac atccaaaacg aatctgacgc
     5461 catgaacagc aaatggactg gaccaagacc aaaagcaata ccaattgttc cagaaacact
     5521 tacctttgag gagtttaagg catatgagga tgttaaaaaa gcaagtgaga ctaatcagat
     5581 ttcacttggt attagcttaa aagaagttga taattgtagc attccacttt cgaaattcaa
     5641 acagttactt tttacttctg acaatccaga taatttgtct aacttgacgc gcttcttagt
     5701 taaatctgtt tcattgatta aagatagtaa ggtttatctt ttagacactg cttctgatta
     5761 tgatgatttg acagaacaac aaattgaata ta
//
LOCUS       DATRQA010000117         5656 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.117, whole genome shotgun sequence.
ACCESSION   DATRQA010000117 DATRQA010000000
VERSION     DATRQA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.117"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            14..1144
                     /locus_tag="VBD27_001637"
     CDS             14..1144
                     /locus_tag="VBD27_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025635.1"
                     /translation="MIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSGKTTLM
                     RMVNHMLKPTNGTLLFKGKDISTINPIELRRRIGYVIQNIGLMPHMTIYENIVLVPKL
                     LKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRALAADQDIILMDE
                     PFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKMVQEGT
                     PNDLLHHPATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITTEKTLTEAITLMRQ
                     KRVDSLLVTDNGKLIGFIDLESLSSKYKKDRLVSDILKHTDFYVMEDDLLRNTAERIL
                     KRGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ"
     gene            1144..1779
                     /locus_tag="VBD27_001638"
     CDS             1144..1779
                     /locus_tag="VBD27_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025636.1"
                     /translation="MVNFLSQYGMQILVKTWEQVYISFFAIALGIAIAVPLGVVLTRF
                     PKVAKIIIAIASMLQTIPSLALLALMIPLFGIGKVPAIVALFIYSLLPILRNTYIGMN
                     NVNPTLKDCAKGMGMKPIQSIFQVELPLATPIIMAGIRLSTIYVIAWATLASYIGAGG
                     LGDLIFSGLNLFQPKLILGGTIPVIILSLIIDYLLGLLETALTPRTTRREA"
     gene            1782..2708
                     /locus_tag="VBD27_001639"
     CDS             1782..2708
                     /locus_tag="VBD27_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7025637.1"
                     /translation="MLKKSHFLQIFTLCLALLTISGCQLTDTKKPGHTTIKVAAQSST
                     ESSIMANIVTELIHHELGYNTTLISNLGSSTVTHQALLRGDADIAATRYTGTDITGTL
                     GLKAVKDPKEASKIVKTEFQKRYNQTWYPTYGFSDTYAFMVTKEFARQNKITKISDLK
                     KLSTTMKAGVDSSWMNREGDGYTDFAKTYGFEFSHIYPMQIGLVYDAVESNKMQSVLG
                     YSTDGRISSYDLEILRDDKKFFPPYEASMVVNNSIIKKDPKLKKLLHRLDGKINLKTM
                     QNLNYMVDDKLLEPSVVAKQFLEKNHYFRGDK"
     gene            2709..3359
                     /locus_tag="VBD27_001640"
     CDS             2709..3359
                     /locus_tag="VBD27_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025638.1"
                     /translation="MKQMNTFQQFIYYFQHNGSYILEQFIHHFLISIYGVILAAIVGI
                     PLGIFIARRGKIKTFVMGIANVIQTIPSLAMISIIMLGLGLGIKTVVATVFLYSLLPI
                     ITNTYTGIRNVDSDLLDAAKGMGMTKRQRLFMVELPLSISVIMAGLRNALVVAIGITA
                     IGAFVGGGGLGDIIIRGTNATNGGAIILAGSLPTALMAIFSDLILGGIQRMLEPRK"
     gene            complement(3421..4602)
                     /locus_tag="VBD27_001641"
     CDS             complement(3421..4602)
                     /locus_tag="VBD27_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7025639.1"
                     /translation="MNINGIKLLSSRAVSKLGDVLYDYGNSTWIASIGGLGQKILGIY
                     QIVELLVSIVLNPFGGALADRFQRRKILLITDAICAIMCFLLSFIGDDKVMVYGLIVA
                     NAILAVSNAFSSPAYKSYIPEIVDKADIITYNANLETIVQIISVSSPVLGFLIFNNFG
                     IRITLIVDAITFLISFLFLYAIKVERVQLSKQEKVAIKNILADIADGFTYIKKEKEIM
                     FFLIIAALLNTFLAMFNYLLPFTNSLLKTSGAYATILSISAIGSIIGALIARKIKSSI
                     NSMLSMLVFSSLGVIVMGFPSLFELPIWISYSGSFLFNSLLTMFNIHFFSQVQIRVDE
                     AYMGRVMSTIFTIAIMFMPIGTLFMTIFSFALSNVSFIVIGCAIAILGGLGFSYSKKQ
                     F"
     gene            complement(4675..5528)
                     /locus_tag="VBD27_001642"
                     /pseudo
     CDS             complement(4675..5528)
                     /locus_tag="VBD27_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MutR family transcriptional regulator"
BASE COUNT         1907 a         1018 c          916 g         1815 t
ORIGIN      
        1 gggccaagaa cctatcatcg aatatcaaaa tatcaataaa gtgtatgggg aaaatgttgc
       61 ggttgaagat attaacctta aaatttaccc tggtgatttc gtttgtttca tcggtacgag
      121 tggatcaggt aaaacaacat taatgcgtat ggttaaccat atgttaaaac caacaaatgg
      181 tactctatta tttaagggaa aagatatctc tactattaac cccattgaat taagacgcag
      241 aattggatat gttatccaaa acattggttt aatgcctcat atgaccattt acgaaaatat
      301 agttcttgta ccaaaattat tgaaatggtc agaagaagct aaaagagcta aagcaaggga
      361 acttattaaa ttagttgaat tacccgaaga atatttggat cgctacccta gtgagttgtc
      421 tggcggtcag caacaacgta tcggtgtcat tcgcgctctt gcagcagacc aagatattat
      481 tttaatggat gagccttttg gagctctgga tcctattact agagaaggta ttcaagactt
      541 agtcaagtct cttcaggaag aaatggggaa aactatcatc ttagttactc atgatatgga
      601 tgaagccctc aagttagcaa caaaaattat tgttatggac aatggtaaaa tggtccaaga
      661 agggacaccc aatgatctct tacatcatcc tgctactagt ttcgttgaac aaatgattgg
      721 agaagagcgt ctccttcatg cgcaggctga tattactcct gttaaacaga taatgttaaa
      781 taatcctgtt tcaataacta ctgaaaaaac actaactgaa gctattacac taatgcgcca
      841 aaaacgcgtt gactcacttc tagtaaccga taacggtaaa ttaattggtt ttattgactt
      901 agaatctcta agcagtaaat ataagaaaga tcgacttgtt tctgatatct taaaacatac
      961 tgatttttat gttatggaag acgacttact tagaaatact gctgagcgta ttttaaaacg
     1021 tggtttaaaa tacgctccag ttgttgacca tgagaataac ttaaagggca ttgttactcg
     1081 tgcatcccta gttgatatgt tatacgatat tatttggggc gatactgaaa cggaggatca
     1141 ataatggtca atttcttatc acagtatggc atgcaaatat tagtaaaaac atgggaacaa
     1201 gtctatatct cattttttgc tatagcactt gggattgcaa ttgctgttcc attgggtgtg
     1261 gtcttaacac gttttcccaa agtagccaaa attatcattg ctatagctag catgctacaa
     1321 actataccaa gtttagcctt attagcttta atgattccat tgtttggaat tggaaaagtc
     1381 ccagctatcg ttgctttatt tatttattcc ttgcttccta tactaaggaa tacttatata
     1441 ggtatgaata atgtcaatcc aacacttaaa gattgtgcta aagggatggg aatgaaacct
     1501 attcaatcta tatttcaagt tgagttacct ttagctactc caattattat ggctggtatt
     1561 cgcttgtcta ctatttatgt tattgcttgg gctacattag cttcttatat aggtgcaggt
     1621 ggacttggtg atctcatttt tagcgggtta aatctttttc agcccaaatt aattctaggg
     1681 ggcacaattc ctgttattat cctgtcactt attatagatt atcttttagg attactggaa
     1741 acagctctaa cgcccagaac aacaagaagg gaggcttaga tatgcttaaa aaatcgcact
     1801 ttttacagat atttacactt tgcttagccc tcttaacgat ttctggttgt caattaaccg
     1861 atactaaaaa acctggtcat accacaatta aggttgctgc ccaaagttct acagagtcta
     1921 gtatcatggc aaatattgtc accgaattaa ttcatcacga attaggatac aacacaactt
     1981 taataagcaa tcttggttcc tctacggtta ctcaccaagc tttgctccgt ggtgatgctg
     2041 acattgctgc cacacgttat acaggaacag acatcacagg aactcttggc ttaaaagctg
     2101 ttaaagaccc taaagaagct tctaagattg taaaaactga attccaaaaa cgctacaatc
     2161 aaacttggta tcctacttat ggtttttctg atacttatgc attcatggtt actaaagagt
     2221 ttgccagaca gaataaaatc accaagatct ctgatctcaa aaagttatca acaactatga
     2281 aggcaggggt tgatagttca tggatgaatc gcgagggaga tggatacact gatttcgcta
     2341 aaacatacgg ttttgaattt tcacatattt accctatgca aattggctta gtctatgatg
     2401 cggttgaaag taacaaaatg caatctgtat taggctactc cactgacggt cgtatttcga
     2461 gctatgattt agaaatttta agggatgata aaaaattctt tcctccttat gaagcctcta
     2521 tggttgtcaa caattctatc atcaaaaaag atcctaaact aaaaaaatta ctccatcgac
     2581 tcgatggtaa aatcaattta aaaacgatgc aaaaccttaa ttatatggta gatgataaac
     2641 ttttagaacc ttcagttgtt gccaaacaat ttttagaaaa aaaccattat tttagaggag
     2701 ataaataaat gaaacaaatg aatactttcc aacaatttat ctactacttc caacataatg
     2761 gtagttatat cttagaacaa tttatccacc actttttaat ttcaatttac ggcgttattt
     2821 tagctgcaat tgttggtatc cctctaggta tcttcatcgc tagacgtggt aaaattaaaa
     2881 cctttgttat gggaatcgca aatgtcatcc aaaccattcc ttctttagct atgatctcta
     2941 tcattatgtt aggtctaggg ctaggtatta aaacagtagt tgcaactgtc tttctttatt
     3001 ctcttctgcc aatcatcaca aatacttaca ctggtattag aaatgttgac tccgacttac
     3061 tagatgctgc taaaggaatg ggcatgacta agcgtcaacg tttatttatg gttgagttac
     3121 cactatctat ttctgttatc atggctggac ttagaaatgc ccttgttgta gcaattggta
     3181 ttacagccat tggtgctttc gtcggtggag gcggacttgg agatatcatc ataagaggaa
     3241 ctaatgcaac aaatggtggt gctattatct tagcaggatc tcttccaaca gcccttatgg
     3301 caattttctc tgatttaatc ctaggcggta tccaacgcat gcttgaacca agaaaataac
     3361 tattaaaaac ttgtcagtag agacaagttt ttttgatgtt tcagaataat ttcttaagtt
     3421 ctaaaattgt tttttactat agctaaatcc taatcctccc aatatagcaa tggcacatcc
     3481 tataacaata aagctaacat ttgataaggc aaatgaaaag atagtcataa atagtgttcc
     3541 tataggcata aacataatag caatagtaaa tatagtcgac atcacacgcc ccatatacgc
     3601 ttcatcgacc ctgatttgta cctgactgaa aaaatgaata ttaaacattg tcagaagact
     3661 attaaaaaga aaactaccac tgtaagatat ccatatcggt aattcaaata gagatgggaa
     3721 tcccataact atgactccca aactactgaa tactagcata gatagcatag aattaatact
     3781 agatttaatt tttctagcaa taagcgctcc aataattgat ccgatagctg atatactaag
     3841 tattgttgca taagcacctg aagttttcaa tagggagtta gtaaaaggta aaagataatt
     3901 gaacattgct aggaaagtgt ttaataaagc agcaataatt agaaaaaaca taatttcttt
     3961 ttcttttttg atgtaagtaa atccatctgc aatatcagct aaaatatttt ttatcgctac
     4021 cttttcttgt tttgaaagct gtaccctttc cactttaata gcataaagaa acaggaaaga
     4081 gatcaaaaaa gtaatcgcat caacaattaa agtaatccta ataccaaaat tattaaaaat
     4141 aaggaaacct aaaacagggg aacttacact aataatttgc acaatggttt ccagattagc
     4201 attataggta atgatatcag ctttatctac tatttcagga atataagatt tataagccgg
     4261 agaagaaaaa gcattggaaa ccgctaagat agcattagca actattagac catacaccat
     4321 tactttatca tctccaataa aagaaagtaa gaaacacatg atagcacaga tagcatctgt
     4381 tattagtaaa atttttcttc gttgaaagcg atcagctaag gcgcctccaa aagggttgag
     4441 gacaatagaa accaacaact ctactatctg ataaatacct aaaatctttt gtcctagacc
     4501 acctatcgaa gcaatccaag tactatttcc ataatcataa agcacatcac caagtttact
     4561 gactgctcga ctagatagta attttatacc attaatattc ataattccct ccttagttcg
     4621 acattatagc aaaccaaaag gagaaatatt aaaaaatccc ataaatggga ttttttataa
     4681 tcttgttacc ctattaaagt gttcctgaaa attttcaaca tgatgtttag aaccaattgc
     4741 ttggaagcaa aaaatagctt gcttcatgct ttgtattcct cgactatccc ctttagcata
     4801 ggcaagaaat cctttttcat agagtaaaat aatacggtga taagcattcc gctcattatt
     4861 taaaagtgct tcaactttct ctagaaaaaa cgttgcttct tctagagcgc gccgttctat
     4921 tagtgtctct accacattta ggagggttgc ttcgacaaca ttgcggtttg tataaatatc
     4981 tcgataatat tccttttgat tgataacctc tttaacaagt tggcaaatat gccttgtgtt
     5041 gtaataccgg taaagatttc caatcaaaat atagtcacta atttcccaac tatccttttg
     5101 aaagaggtaa tccgacaaaa agcaaaggtc ttcttccgac atttttcttg aggagtcata
     5161 ttcacatatc attcctctaa aaagaactat gtttaaacga caacgtatat ctgaactatc
     5221 cttttttagt ttaccttctt ccacagaaat catcttttct aacccagcta cgtctctttg
     5281 ataatgatat tgagccatct gagacattac tgatcagatt tttgatattt atttactcta
     5341 tccataaatt cgctaattga taaatcaatt gcctctaaag cccctaaaaa ttttgttaat
     5401 gataaatctg attcacccct ctcaaatcgt gataactgag aaacagaaag ttcatttgaa
     5461 gcaacctgtt taagagaaaa ctgtcgatta agtcgaaatt ccctaaaaat agggccatat
     5521 ataaacatta gtattccttt ctaaccgtaa tctcattggt aaacttatga ggtaatttca
     5581 ataatttata tttaccaact ttaaccaata tatgagctga tatatagtca ttttctttta
     5641 tttgtaatat catatc
//
LOCUS       DATRQA010000118         5646 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.118, whole genome shotgun sequence.
ACCESSION   DATRQA010000118 DATRQA010000000
VERSION     DATRQA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.118"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            99..344
                     /locus_tag="VBD27_001643"
     CDS             99..344
                     /locus_tag="VBD27_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO7025640.1"
                     /translation="MGLIWSLIVGAIIGAIAGAITNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLADMALIPSIVGAIIVIIVTSFVLGKKS"
     gene            392..640
                     /locus_tag="VBD27_001644"
     CDS             392..640
                     /locus_tag="VBD27_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO7025641.1"
                     /translation="MSLIWSLIVGAIIGAIAGAVTNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLAGMALIPSIVGAIIVVIVTSFVLGKMNN"
     gene            655..1236
                     /gene="amaP"
                     /locus_tag="VBD27_001645"
     CDS             655..1236
                     /gene="amaP"
                     /locus_tag="VBD27_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO7025642.1"
                     /translation="MSKGLKSLYTLLGLISLTLLGFVAVISKQHIYLPSFNWLDWDFN
                     LPSPIDVGMYHYFFWGALVLFVIVLLAILVVLFYPRRYTEYKLADKTGKLMLKKSAIE
                     GFVKTEVLKTGLMKSPSVTAHLYKKKVKVDVKGLLTSRTNVPEQLEHIQSDVELGLKE
                     FFGLEKKMNTRVFVKQVEEENVGNAKTNKSRVE"
     gene            1246..1440
                     /locus_tag="VBD27_001646"
     CDS             1246..1440
                     /locus_tag="VBD27_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO7025643.1"
                     /translation="MSEFVRKYRYPLGGAVIGLVLAAMIVTIGFFKTILALVIIVLGA
                     YAGLYVQRTGMLDQFFNKRK"
     gene            1496..2038
                     /locus_tag="VBD27_001647"
     CDS             1496..2038
                     /locus_tag="VBD27_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7025644.1"
                     /translation="MTETYIKNTTNNSGTTAVRGELTFEDKVIEKIVGIAIEHVDGLL
                     AVNGGFFSNLKNSVVNSDSVTDGVNVEVGKKQVAVDLDIVAEYQKHVPTIFADIKKVV
                     EAEVKRMTDLEVVEVNVNVVDIKTRAQHEEDSVTLQDRVTSAAQATGEFASNQVSNVK
                     SAVGNGVDKVEDMKSEPRVQ"
     gene            2085..2282
                     /locus_tag="VBD27_001648"
     CDS             2085..2282
                     /locus_tag="VBD27_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7025645.1"
                     /translation="MSEEKFDAKVDKVSGSVKESVGKLTGDKEVESEGKVDKLKGHAK
                     EKLADIKDTIKGASESFKKKD"
     gene            2304..2855
                     /locus_tag="VBD27_001649"
     CDS             2304..2855
                     /locus_tag="VBD27_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7025646.1"
                     /translation="MENQKQDIKTSVTYEEKVIAKIVGHALESVDGLLAVSGGFFSNL
                     KNSVVNSDSVTDGVNVEVGTKEVAVDLDIVVEYGKDIPAIVESIKAIVSQNVEVMTHL
                     KVVELNANVVDVKTKAEHEADSVTVQDRVSDAAQATGNFASEQAGKAKAAISSGAEKT
                     KEAVSNGTEAAKEKISEARTSES"
     gene            3019..3660
                     /locus_tag="VBD27_001650"
     CDS             3019..3660
                     /locus_tag="VBD27_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7025647.1"
                     /translation="MVSEQSEIVTSKYQKIAVAVAQRIANGDYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLQILTLKHGSGAIILSKEKAIAFLNQYETSHSVAILKGKIR
                     DNIKAQQQEMEELATLVDDFLLQTRAVSKQYPLAPYEIIVSEDSEHLGKSIGELNVWH
                     QTGATIVAIEHEGKFIVSPGPFSVIEQGDHIFFVGDEDVYARMKTYFNLRMGL"
     gene            3665..4429
                     /locus_tag="VBD27_001651"
     CDS             3665..4429
                     /locus_tag="VBD27_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO7025648.1"
                     /translation="MDFVFDIDGTLCFDGMSLSKEIQGILERAQIDYGHRVTFATARS
                     YRDTIGILGDKLSLSKIIGLNGATLHENGHLVDSYYLQSDFFSTIISYCHRYQIPYFV
                     DEVFNYATYQASKIPFIAYVDPQKRGELLEVSKIEKPIKMVLYFGDQLRRADQMLAKL
                     NRFGLSSHFFHEFEKCLYINPIAVDKGKATKKLFGNRFIAFGNDKNDISMFDAAHYSV
                     QVGDFDELTPYANLRVSRESVHEGITMLFEKYKGIT"
     gene            4429..5088
                     /locus_tag="VBD27_001652"
     CDS             4429..5088
                     /locus_tag="VBD27_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEO7025649.1"
                     /translation="MDLFLSLERKFKAASDKEVSKQQETYLRHHFKCYGIKSPERRML
                     YKELIKAAKRQAKIDWQLLDKCWQSDYREYHHFVLDYLLAMSQFLTYNDCSRLEFYAR
                     HQQWWDSIDVLTKIFGNLSLKDDKVMNLLSEWSLDQDFWMRRLAIEHQLGFKEKTNTD
                     ILSLFILRNTGSQEFFINKAIGWALRDYSKYNKVWVKDFISNHYDELSTLSIREGSKY
                     L"
     gene            5161..>5646
                     /gene="tpx"
                     /locus_tag="VBD27_001653"
     CDS             5161..>5646
                     /gene="tpx"
                     /locus_tag="VBD27_001653"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEO7025650.1"
                     /translation="MTTFLGNPVTFTGKQLQVGDIAKDFLLIATDLSQKSLKDFEGKK
                     KVISVVPSIDTGICSKQTRTFNEELSELDNTVVITVSMDLPFAQKRWCSAKGLDNVIL
                     LSDFYDHSFGQEYALLMNEWHLLTRAVLILDEHNKVTYTEYVDNVNSDVDYEAAINAA
                     KI"
BASE COUNT         1872 a          765 c         1183 g         1826 t
ORIGIN      
        1 attaattgcc gagtggtcac tttagaaact cttgttattt agttaagaac aaaataagtt
       61 tgtacactga gatgtactta ggaagaaagg tatttatcat ggggttaatt tggtcattaa
      121 tcgttggtgc tattattggt gctattgcag gagcaatcac taacaaaggt ggttcaatgg
      181 gttggatagc taacatctta gctggtttag ttggttcatt tgttggtcaa agtttactag
      241 gaacatgggg gcctaagtta gcagatatgg ctttgattcc ttcaatcgta ggtgcgatta
      301 tcgttatcat tgttacttca tttgtattag gcaaaaaatc ataactacat aactagtaga
      361 atgattagtc aagaaagaga aggtatttat catgagttta atttggtcat taatcgtagg
      421 tgctattatc ggtgctattg caggggctgt tactaacaaa ggtggttcaa tgggttggat
      481 agctaacatc ttagctggtt tagttggttc atttgttggt caaagtttac taggaacatg
      541 ggggcctaaa ttagcaggta tggctttgat tccttcaatc gtaggtgcga ttattgttgt
      601 cattgttact tcatttgtac taggtaaaat gaataactag aaaaggagtt acctatgtca
      661 aaaggactaa aaagtttata tactttgcta ggtctaatct cccttaccct actaggattt
      721 gtagctgtta tcagtaaaca acacatttat ctaccatctt ttaattggtt agattgggat
      781 tttaatttgc catcaccgat tgacgttggt atgtatcatt atttcttttg gggagcatta
      841 gtgctgtttg taatcgtttt actggctata ttggtagttc tgttttatcc aagacgatac
      901 acagagtata aactggctga taaaactggt aaattgatgt taaaaaaatc tgcaatagag
      961 ggatttgtta aaacagaagt attaaaaact ggtttgatga aatctcctag tgtgacagct
     1021 catttatata agaaaaaagt gaaagttgat gttaaaggtc ttttaacttc tcgtacaaat
     1081 gtaccggaac aacttgaaca tattcaaagt gatgttgaac ttggcttaaa agaattcttt
     1141 gggttggaaa agaaaatgaa cactagagta tttgttaagc aagttgaaga agaaaatgtt
     1201 ggaaatgcta agacgaataa aagtcgtgta gaataggagt ctaatatgtc agaattcgtg
     1261 aggaaataca gatatccact aggaggtgca gttattgggc tcgttttagc tgcaatgatt
     1321 gtaaccattg gattctttaa aacaattctt gctcttgtta tcattgtgct aggtgcttat
     1381 gctgggcttt atgtgcaaag aacaggtatg cttgaccaat tctttaataa gagaaaataa
     1441 aatttttcgt ttatatttat tctacattat ttaaataaaa gaaagaggaa ctattatgac
     1501 tgaaacttac atcaaaaaca caactaacaa ttctggcacg acagctgttc gtggagaatt
     1561 aactttcgaa gataaagtga ttgaaaaaat tgttggaatt gcgattgagc atgtagatgg
     1621 tcttttggct gtaaatggtg gttttttctc aaatttaaaa aatagcgttg taaattctga
     1681 ttcagttaca gatggcgtaa atgttgaagt gggtaaaaag caagttgctg ttgatttgga
     1741 tattgttgct gaataccaaa aacatgttcc aactatcttt gccgatatca aaaaagttgt
     1801 tgaagctgaa gtgaaacgta tgacagacct tgaagtggtt gaagttaatg ttaatgttgt
     1861 tgatattaaa actcgtgctc aacatgaaga agattcagta actcttcaag atcgtgtaac
     1921 aagtgcagca caagcaacag gtgaatttgc ttcgaatcaa gtttcaaacg ttaaaagtgc
     1981 cgtaggtaac ggtgttgata aagtggaaga catgaagtct gaaccacgtg tacaataatt
     2041 gatttaagta tagtttaaag tattgttatt agtaggaggc tattatgtct gaagaaaaat
     2101 ttgatgcaaa agttgataag gtttctggtt cagtaaaaga atctgttggg aaacttactg
     2161 gcgataaaga agttgaatct gaaggtaaag tcgataaatt gaaaggtcat gcaaaagaaa
     2221 aacttgctga cattaaagat accattaagg gtgcttcaga gagctttaag aaaaaggact
     2281 agaaggtttg aggtcattta tttatggaaa atcaaaaaca agatattaaa acttcagtaa
     2341 cttatgaaga aaaggtaatt gctaaaattg ttggacatgc tttagaatca gtagacggac
     2401 tcttggctgt gagtggaggt ttcttctcta atctaaaaaa tagcgttgta aattctgact
     2461 cagtaactga tggtgtaaat gttgaagttg gtactaaaga agttgcagtt gatttagaca
     2521 tcgttgttga atacggcaag gatatcccag ctatcgttga aagcattaaa gcgattgtca
     2581 gtcaaaacgt tgaagtgatg acccacctta aagttgttga attgaacgca aatgtcgtag
     2641 atgttaagac taaagctgaa catgaagctg actcagtgac agtacaagat cgtgtatctg
     2701 atgcagcaca agcaacagga aactttgctt ctgaacaagc tggcaaagct aaagcagcaa
     2761 tttcttctgg agcagagaaa acaaaagaag cagtatcaaa tggtactgag gctgcaaaag
     2821 aaaaaatctc agaagctcgt actagtgaat cttaattcag taaactactt tttatgataa
     2881 gacactcgga gctgggagac cagctccttt tatatgatat ttactaaaga cattagatat
     2941 taacaaaaaa gatatatttg caatattttg ctaattgtgg tttaatatgt atgtttttat
     3001 taggataagg agggttggat ggtttctgaa caatctgaaa ttgtaacatc taaatatcaa
     3061 aaaatagcag tagcggttgc acaaagaatc gcaaatggag attatgaagt tggagagaag
     3121 ttgaaatcta gaacaactat tgcttcaaca ttcaacgtat caccagaaac agcacgtaag
     3181 ggtcttaata ttttagcaga tttacagatt ttaactttaa agcatggcag tggagccatt
     3241 attctttcta aagaaaaagc aattgcattc ttaaaccaat atgaaacctc tcattcagtt
     3301 gctattttga aaggtaaaat ccgtgataat attaaggctc agcaacaaga aatggaagag
     3361 ttagctactt tagttgatga ttttttgctt caaacacgtg cggttagtaa acaataccca
     3421 ctagcaccat atgaaattat tgtcagtgaa gattctgaac accttggtaa gtcaatagga
     3481 gaattaaatg tatggcatca aactggtgcg actattgtgg ctattgaaca cgaagggaaa
     3541 tttattgttt ctccagggcc tttctcagtt atagagcaag gagatcatat cttttttgtt
     3601 ggtgatgaag atgtttatgc acgtatgaaa acctatttta acttaaggat ggggctttga
     3661 gagaatggat tttgtctttg atatagatgg aactctttgt tttgatggta tgagtctatc
     3721 taaagaaata caaggaatac tagagagagc ccagattgat tatggtcata gagtcacttt
     3781 tgctacggcg cgatcttata gagacactat aggtatcctt ggcgacaagc tatctttatc
     3841 aaaaattatt ggtttaaatg gtgcgacttt acatgaaaat ggtcacttag tggatagtta
     3901 ctatttacaa agtgactttt ttagtactat tatttcatat tgtcatcgct atcagattcc
     3961 atattttgtt gatgaggtct ttaattacgc tacttatcag gcttctaaaa ttccctttat
     4021 tgcttatgtt gatcctcaaa aaaggggaga gcttctagaa gtttctaaaa ttgagaaacc
     4081 gataaaaatg gtgttgtatt ttggtgatca gctgagaagg gcagaccaaa tgttagcgaa
     4141 attaaatcgt tttggactta gtagtcactt tttccacgaa tttgaaaaat gtttgtacat
     4201 aaatcccatt gcagttgaca agggaaaagc cactaagaaa ttatttggga atcgttttat
     4261 agcttttggc aatgataaaa atgatatttc tatgtttgat gctgcgcatt atagtgttca
     4321 agtaggggat tttgatgaac tgactcctta cgctaattta cgtgtatcaa gagaatctgt
     4381 acatgaagga attacaatgc tttttgaaaa atacaagggg attacgtaat ggatttattc
     4441 ctaagtttag agaggaaatt taaagctgca agtgacaaag aagtttctaa gcaacaggag
     4501 acttatttac gccatcattt taagtgctat ggtattaaat cacctgagcg acgtatgctc
     4561 tacaaagagc ttataaaggc cgctaaaaga caagcaaaaa ttgattggca attgcttgat
     4621 aaatgttggc aaagtgacta tcgtgaatat catcattttg tacttgatta tctattagct
     4681 atgagtcaat ttctcaccta taatgattgc tctagactag agttttacgc tcgtcatcaa
     4741 cagtggtggg atagtattga tgttctgact aagatttttg ggaacttatc cttaaaagat
     4801 gacaaggtta tgaatttact gtcagaatgg tcacttgatc aagatttttg gatgaggcga
     4861 ctagctattg agcatcaact aggatttaaa gaaaaaacaa atactgatat tttgtcactt
     4921 tttattttaa gaaatacagg aagtcaagaa ttttttatca ataaggcgat tggctgggcg
     4981 cttagggatt attcaaagta taataaagta tgggtaaagg actttatcag taatcattat
     5041 gatgagttgt cgactttgag catacgcgaa ggaagtaagt acctctaatt atcagttcaa
     5101 ttattcgaaa aagaagaagc ttcatagtaa aataaaggta attgataagg agataaactt
     5161 atgacgacat ttcttggaaa tcctgttact tttacaggaa aacagctaca agttggggat
     5221 attgcaaaag attttttact aatagctaca gacttatctc aaaaatcttt aaaagatttt
     5281 gaagggaaaa agaaagttat tagcgttgtt ccttctattg atacaggtat ttgttcaaaa
     5341 caaacacgta cttttaatga ggaattatca gagctagata acacagtggt tattactgtt
     5401 tctatggacc ttccatttgc tcagaagcgt tggtgcagcg ctaaaggtct tgataacgtg
     5461 attttattat cagattttta tgatcattcg tttggtcaag agtacgctct tttgatgaat
     5521 gagtggcatt tgttaacgcg tgcagttctt attttggatg agcataataa ggtgacttac
     5581 accgaatacg ttgacaatgt taattcagat gttgactacg aagcggcgat taatgctgca
     5641 aagata
//
LOCUS       DATRQA010000119         5617 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.119, whole genome shotgun sequence.
ACCESSION   DATRQA010000119 DATRQA010000000
VERSION     DATRQA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.119"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(125..298)
                     /gene="secE"
                     /locus_tag="VBD27_001654"
     CDS             complement(125..298)
                     /gene="secE"
                     /locus_tag="VBD27_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO7025651.1"
                     /translation="MFVKGIFQVLRDTTWPNRKQRWKDFISILEYTVFFTIVIYIFDK
                     LLAAGVMDLINRF"
     gene            complement(334..486)
                     /gene="rpmG"
                     /locus_tag="VBD27_001655"
     CDS             complement(334..486)
                     /gene="rpmG"
                     /locus_tag="VBD27_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7025652.1"
                     /translation="MAQKKASLACTECGSRNYSIGVSSTPKPTRLEVNKFCKYCKKYT
                     IHKETR"
     gene            complement(535..2856)
                     /locus_tag="VBD27_001656"
     CDS             complement(535..2856)
                     /locus_tag="VBD27_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7025653.1"
                     /translation="MKLFDKFIDLFRVDEDNDEMTRKNEQETREETSNLDGEEVYDID
                     DITRPSKSQYQRGIRHQKENAKSRLEWLQKVDRYLPSPKNPIRRFWRRYRIGKLLFIA
                     LMAFILIFGSYLFYLSKTATVSDLQSALKTTTTIYDKNKEYAGKLSGQKGTYVELNAI
                     SDHLKNAVIATEDRTFYENNGVNFKRFFLAVATLGKFGGGSTITQQLAKNAYLSQDQT
                     IKRKAREFFLALELTKKYSKAEILTMYLNNSYFGNGVWGVEDASRKYFGTSAANLTVD
                     EAATLAGMLKGPEVYNPYYSVENATNRRDTVLAAMVDAGQLTKSQAKEAASIGMKNRL
                     ADTYAGKINDYKYPSYFDAVVNEAIDTYGISEKDIVNNGYKIYTALDQNYQSGMQKTF
                     DDTSLFPVSDYDGQSAQGASVALDPKTGGVRGLVGRVQSTKDAQFRSFNYATQSKRSP
                     ASTIKPLVVYSPAIASGWSIDKELPNKVQDFHGYKPSNYGGIETESIPMYQALANSYN
                     IPAVYTLDKLGINKAFTYGRKFGLNMSSANKELGVALGGSVTTNPLEMAQAYSTFAND
                     GIMHRAHLITRIETANGKLVKQFTDKPKRVISRSVASKMTSMMLGTFSNGTAINANVY
                     GYTMAGKTGTTETDFNPNLSGDQWVVGYTPDVVISQWVGFKNTDKHHYLTDSSAGTAS
                     NIFSTQASYILPYTKGSSFTHIENAYFQNGIGSVYNAQDASNTTNQESRSIINDLKDS
                     ASKAAQDISRAVEDSNFQEKVKDAWNSLKDYFR"
     gene            2900..3784
                     /locus_tag="VBD27_001657"
     CDS             2900..3784
                     /locus_tag="VBD27_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7025654.1"
                     /translation="MSFSLKIRNPYGEHTVKELLEDYFLIPRKIRHFLRVKKHVLINN
                     EFINWQTVVQENDTITLIFDDEDYPTKKIPLGRAELIDCLYEDEHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSAYSGQTCYVVHRLDMETSGAVLFAKNPFILPLINQRLERKEIWR
                     EYWALVEGKFSPKHQVLRDKIGRNRHDRRKRIIDSKNGQHAMTIIDVLKYIQNSSLIK
                     CRLETGRTHQIRIHLSHHGHPLIGDPLYNPSSNNERLMLHAHRLTLSHPLTCETISVE
                     APSSTFEKVLNNYKKGVG"
     gene            complement(3809..5449)
                     /locus_tag="VBD27_001658"
     CDS             complement(3809..5449)
                     /locus_tag="VBD27_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025655.1"
                     /translation="MFDFFKKKVVKVCLVIFGIVLVSLLSLGFFYFSKGQVLSRFVAA
                     RSRTSGQAFDNIKEYMVWSDTGESITNDEANYANFEPLSKSEARKLGQEIKEGNKNDS
                     MYLKRVGSRLGIFPDYRIANKPMSLTLKTNVPKLDVLLNQKKVATSNSDHFSVTVERL
                     PRTHYTASLEGTSDGKEIKLKKDYDGKNQTIDLSVAFKSFTVTSNLMDGNLYFGDNRI
                     AKLKDGSYSVENYPVTDGSKAYIKKVFNDGEITSHKQKLISIADNQTIKLDVDGLLNE
                     KEAGQKLITAFNQLILYVSTGQDPQTLGTVFEKGAENDFYKGLKESIKAKFVTDNRKA
                     SHFTIPNIVLNKMTQVGKESYQVNFAADYDFNYDKSTDPDKKTYGHIIQNLTGNFIMK
                     KSGNSYLISNDGKKDITVAKETNKVKADPVFIFPENLVGSWKGEVEDGTVTMTFDKDG
                     KVTQKKVYKDSKSKESNHSAKVTKLEDKGNGLYLYQYESGTDTTTFVTGGIGGLKVKY
                     AYGIKIEGNKIIPVIWQTSSDGEFDYHKPLLSKPLTKQ"
BASE COUNT         1764 a         1084 c          854 g         1915 t
ORIGIN      
        1 tagtaaaaac aatcaaaaga ttattttttt ataatttaat cggatatata tgttattttc
       61 cacacaaaaa aacccttagt aaaatctaag ggtttgggtt gtttctatct aaaataatct
      121 attattaaaa tcgattaatc aagtccatga ctcctgcagc taacagtttg tcaaagatgt
      181 agataacgat tgtgaaaaat acagtgtatt ccaagatgga aataaaatct ttccaacgtt
      241 gttttcgatt tggccaagta gtgtcacgca atacttgaaa aatccctttg acaaacatta
      301 tcagttctcc tttttgcagt ttgttacgct atatcaacgc gtttctttgt gtattgtata
      361 tttcttacaa tatttacaaa atttatttac ttctagtcgt gttggcttgg gggtgctact
      421 tactccgatt gagtaatttc ggcttccgca ttctgtacac gctaggcttg ctttcttttg
      481 tgccataacg cctccataag tgttatttta acatgatttg tataatcctg caagctatct
      541 aaagtagtcc tttagactat tccaagcatc ttttaccttc tcttggaaat ttgaatcttc
      601 cacagctcta gaaatatctt gtgcagcctt acttgccgaa tcttttaaat cattaataat
      661 tgagcgtgat tcttggttcg ttgtattact agcatcttgt gcattataaa ctgaaccaat
      721 accgttttgg aaataagcat tctcaatatg agtaaaacta gaccccttag tatacggtag
      781 tatatatgaa gcttgcgtgc taaatatatt tgaagctgta cctgcactag aatctgtcaa
      841 ataatggtgt ttatctgtgt tcttaaagcc aacccattga ctaataacga catcaggcgt
      901 atagccgact acccactggt ctccagataa attggggtta aaatctgttt cggttgttcc
      961 agttttacct gccattgtat aaccataaac attggcatta atagctgtac cgtttgagaa
     1021 tgttcctaac atcatactag tcattttact agccactgag cgactaataa ctcgcttagg
     1081 cttatctgta aattgcttta ctaattttcc attagctgtc tcaattcgag taatcaaatg
     1141 agcgcgatgc attataccat catttgcaaa agttgagtaa gcttgtgcca tttctaaagg
     1201 attagtagtt acacttccac ctagcgccac tcctagctct ttgtttgctg atgacatatt
     1261 tagaccaaac ttcctgccat aagtaaaagc tttattgatt cctagtttat ctaaagtata
     1321 aacagcagga atattataag aattagctag tgcttgatac atcgggatac tttcagtctc
     1381 aattccacca tagttacttg gtttataacc atgaaaatct tgaactttat taggaagttc
     1441 tttatctatt gaccatcctg aagcaatagc tggtgaataa acaactaaag gttttatggt
     1501 tgaggctgga cttctctttg attgagtcgc gtagttaaaa cttctaaact gtgcatcttt
     1561 agtactttga acacgtccaa ctaaacctct tacaccacca gtttttggat ctagagctac
     1621 gctagcacct tgggcagatt gaccatcata gtcagataca gggaagaggc tagtatcatc
     1681 aaatgttttt tgcattcctg attgataatt ttgatctaat gcagtgtaaa tcttatagcc
     1741 attattgaca atatcttttt ctgaaatacc ataagtatca atggcttcat tgacaacagc
     1801 atcaaaatag gatgggtact tgtaatcatt aattttacca gcataggtat ctgctaatct
     1861 atttttcata ccaattgaag cagcttcttt agcttgagac ttggttagtt gacctgcatc
     1921 aaccatcgct gccaaaacag tatctcttcg gttagttgca ttttcaacag aataataagg
     1981 attataaact tctggtcctt taagcattcc agcaagtgta gcagcctcat ctactgtcaa
     2041 attagcagca ctggttccaa aatatttcct actagcatct tccacacccc aaacaccatt
     2101 accaaaataa gagttattaa gatacatggt cagaatctca gccttgctat atttcttagt
     2161 caattctagc gctaaaaaga attcacgcgc cttgcgttta atcgtttggt cctgtgacaa
     2221 gtaagcattt tttgctaatt gctgggtaat agttgagcct ccgccaaatt tcccaagtgt
     2281 agctactgcc aaaaaaaatc gcttaaagtt tacaccatta ttttcataaa aagttctatc
     2341 ttcagttgct ataacagcat ttttgagatg atcagaaata gcatttaact ctacataagt
     2401 tcccttttgt cccgaaagtt ttccagcgta ttctttattc ttatcataaa tggtggtggt
     2461 tgtttttaga gcactctgta aatcactaac tgtagcagtt ttagaaagat aaaaaagata
     2521 gctaccaaat ataagaataa aagccattaa ggcgataaaa agcaacttcc caatacgata
     2581 gcgccgccaa aaacgacgga taggattctt aggagaaggg aggtaacgat caactttttg
     2641 tagccattca agtcgagatt tagcattttc cttttggtga cgaatgcctc gctgatactg
     2701 ggatttacta ggtcgggtaa tatcatcaat atcatagact tcttcgccat ccaaattgct
     2761 agtttcttct ctagtttctt gttcattttt tcttgtcatt tcgtcgttgt cttcatcaac
     2821 acgaaataag tctataaact tatcaaataa tttcatagac ttattatatc aattttcaat
     2881 aaaatgctat aataaaacca tgtcattttc attaaaaatt agaaatccat acggtgaaca
     2941 taccgttaaa gaactccttg aagattattt tttgattcca cgtaagatta gacatttttt
     3001 gcgtgttaaa aaacatgtac ttataaacaa tgaattcatt aattggcaaa ctgtcgtcca
     3061 agaaaacgat actattacct taatctttga tgatgaggat taccctacta aaaaaattcc
     3121 tctgggcaga gcagagctta ttgattgtct ttatgaggat gaacatctta ttatcgttaa
     3181 taaacctgaa ggtatgaaaa ctcacggtaa ccaaccaaat gaaatagcac tgttaaatca
     3241 tgtatctgcc tattctggac aaacatgcta tgttgttcat cgcctagata tggagaccag
     3301 tggagctgtt ttatttgcta aaaatccatt tatacttccc cttatcaatc aacgcttaga
     3361 acgaaaagaa atttggcgtg aatattgggc tttagttgaa ggaaaatttt cacctaagca
     3421 tcaagttttg agagacaaaa ttggacggaa ccgtcatgac agacgtaaac gaatcattga
     3481 ttctaaaaac ggtcaacatg ctatgacaat cattgacgtt ttgaagtata tccaaaatag
     3541 tagtctcata aaatgccgac tggaaaccgg aagaacccat caaattcgca ttcacttatc
     3601 tcatcacgga catcctttaa taggagatcc cctctacaac ccttcttcta ataatgaaag
     3661 gttaatgcta cacgctcacc gattgactct atcccatcca ttaacttgcg aaactattag
     3721 cgtagaggcc ccttcatcta ctttcgagaa ggttttaaac aattataaaa aaggagttgg
     3781 ataataatta tccaactcct tttaatcatt attgcttagt taatggctta ctgagtagtg
     3841 gtttatggta atcaaattct ccatcactag atgtctgcca gattactggg atgatcttat
     3901 tcccttcaat ttttattcca taggcgtatt taactttaag accaccgata cctcctgtta
     3961 caaaagttgt agtatcagta cctgattcat attgataaag gtagagccca tttcccttat
     4021 cctccaactt cgtaacttta gcagaatggt tactttcctt agatttacta tccttgtaaa
     4081 cttttttctg agtaacttta ccatccttat caaaagtcat cgttactgta ccgtcctcaa
     4141 cttcaccttt ccaacttcct acaaggttct ctggaaaaat aaaaacggga tcagccttaa
     4201 ctttattagt ttccttagct actgtgatat cttttttacc atcattagaa ataaggtaac
     4261 tatttcccga ttttttcata ataaaattac cagttaaatt ctgaataatg tggccatatg
     4321 ttttcttatc aggatctgtt gatttatcat aattaaaatc ataatcggca gcaaaattaa
     4381 cttgataact ttctttccca acctgtgtca ttttattaag gacaatatta ggaatagtga
     4441 aatggctcgc tttacgatta tcagttacaa attttgcttt aatactttct ttcaatcctt
     4501 tgtaaaaatc attttcggca cctttttcaa aaactgttcc taaagtctga gggtcttgcc
     4561 cagttgatac ataaagtatc aattgattga aagcagttat caatttctgt ccagcttctt
     4621 tttcatttaa taaaccatca acatctaatt tgattgtctg attatcagcg atagagatta
     4681 atttttgttt atgagaggtt atctcaccat cattaaaaac ttttttgata taagcttttg
     4741 aaccatcagt cactggataa ttctctacgg aatagctacc atcttttaat ttagcaatac
     4801 gattatcacc aaaataaaga ttaccatcca taagattact tgttactgta aaagatttaa
     4861 aagcgaccga taaatcaata gtttggtttt taccatcata atcttttttg agtttaatct
     4921 cttttccgtc actagtccct tcaaggctag cagtataatg ggttctaggt aaacgttcca
     4981 ccgttactga aaaatgatct gaattgcttg tcgcgacctt tttttgattc aacaaaacat
     5041 ccaatttagg aacattggtt ttcaaggtca aagacatagg cttattagca atacgataat
     5101 cagggaatat acctaagcga ctacctacac gctttagata cattgaatca tttttgtttc
     5161 cttcttttat ttcctgccct aatttacgcg cctcactttt agacaaaggc tcaaaattag
     5221 cgtaattggc ctcatcattt gtgatagatt ctcccgtatc tgaccaaacc atgtattctt
     5281 taatattatc aaacgcttga cctgacgtac gagaacgtgc tgctacaaaa cgactaagta
     5341 cttgtccctt tgaaaaataa aagaatccta gactcagaag tgatactaaa actataccaa
     5401 aaataactaa gcacacttta acaacttttt tcttaaaaaa atcaaacata ccttatcctc
     5461 tcattcactt tatattaaag taaattgctt aaagaaaagt tacaacactg ataaaacagc
     5521 tgtatttgta cttgtaaccg ctatttttct attataacaa aacgaaaaaa ggattgaaac
     5581 cataatccga tttcaacctc taaaattttt gtctatg
//
LOCUS       DATRQA010000120         5587 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.120, whole genome shotgun sequence.
ACCESSION   DATRQA010000120 DATRQA010000000
VERSION     DATRQA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.120"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            162..1304
                     /gene="tgt"
                     /locus_tag="VBD27_001659"
     CDS             162..1304
                     /gene="tgt"
                     /locus_tag="VBD27_001659"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO7025656.1"
                     /translation="MTDHPIKYRLIKQEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIAKAGGLHKFMNWDQAILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGAKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLNAHRRPHDQGLFGIVQGAGFEDLRRQSARDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTVPMLPNDKPRYLMGVGAPDSLIDAVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKFAEDFTPLDPNCDCYTCKNYTRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKDVRQAIMDDNLLEFRQDFMERYGYGTNNRNF"
     gene            1410..1721
                     /locus_tag="VBD27_001660"
     CDS             1410..1721
                     /locus_tag="VBD27_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025657.1"
                     /translation="MMQEYFGIGLDSSSRCYHYHTKLDIVALKCAVCQKYYACYKCHD
                     ALEEHCFVATKSDEMFPVLCGSCRQMLTLKEYKTGFCPYCRMLFNPNCQRHDSIYFSK
                     E"
     gene            1728..2267
                     /locus_tag="VBD27_001661"
     CDS             1728..2267
                     /locus_tag="VBD27_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO7025658.1"
                     /translation="MFHTKSLIQMAMMATLLVVLGFIPALPLGFIPVPIVLQNLGVMM
                     AGVILGGRKGSLSILLFFLVGLVIPVFSGLRSTIPVLTGPTAGYVIAWLFVPLLISYG
                     VKLVNKKNFIAIFLIVWLAGVLFVDVAGAVWLASYMNIPLDKSLLQNLVFLPGDTIKA
                     IIAAVVAVKYKDSFFLTEK"
     gene            2406..3182
                     /locus_tag="VBD27_001662"
     CDS             2406..3182
                     /locus_tag="VBD27_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7025659.1"
                     /translation="MKLTTLGCWGGYPYKDGGTTSFLLTGENNFHLLIDAGSRSINEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLYHYRLLYPKDKWEPKVLPVYGHNEDDYEFS
                     KLTLPNVAQGIAYHTNGVEQVGPFNITFIKTVHPVVCYAMRITERETGQVLVFTGDSG
                     YFADLVPFTKGADLFLADVYLYEGNENHIAHLTSKEAGEIAYNAKVKKLVLTHVPQFP
                     GEGINPENHLEELRLQSEKYAGGISVDLAQPHKSWDLGKL"
     gene            3182..3688
                     /locus_tag="VBD27_001663"
     CDS             3182..3688
                     /locus_tag="VBD27_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEO7025660.1"
                     /translation="MQKFTQEEKEWFMTEALKEAKSSLEKEEIPIGCVIVKDGHIIGR
                     GHNAREEFNKAILHAEIMAINNANEKVGNWRLLDTTLFVTVEPCVMCSGAIGLARIPH
                     VIYGAKNTKFGAAGSLYDILSDSRLNHRVEVETGILEDQCAKIMQDFFRKGRQRKKEA
                     KLSEKTKI"
     gene            3723..3808
                     /gene="ffs"
                     /locus_tag="VBD27_001664"
     ncRNA           3723..3808
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VBD27_001664"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3960..5309
                     /locus_tag="VBD27_001665"
     CDS             3960..5309
                     /locus_tag="VBD27_001665"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO7025661.1"
                     /translation="MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSD
                     FLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFAN
                     LQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEK
                     PRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAY
                     TLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
BASE COUNT         1800 a          885 c         1113 g         1789 t
ORIGIN      
        1 ttcatttcat tataacacat tccttaagat gagcatgaca agagtacatt tttttgataa
       61 aatcttaatg gctaaaaaag aaattaatac aaagtattac atttagatat tgattagcac
      121 taaagcccat gactgttttt catggaagca aggagaaaac gatgactgat catccaataa
      181 aatatcgatt aattaaacag gaaaaacaca caggagccag acttggtgaa ataatcacac
      241 cacatggtac atttccgaca ccaatgttta tgcctgttgg gacacaagca actgttaaga
      301 cacaatctcc tgaagaacta aaagaaatgg gttcaggaat tattctatca aatacctacc
      361 atttatggtt acgtccaggt gatgaattga ttgctaaagc tggaggttta cataaattca
      421 tgaactggga tcaggctatt ctaacagaca gtggaggctt tcaggtttat tctttggctg
      481 atagtcgaaa catcactgaa gaaggagtaa cctttaaaaa ccatttaaat ggtgcgaaaa
      541 tgtttttatc accagaaaaa gctatttcaa ttcaaaataa ccttggttct gatattatga
      601 tgagttttga tgagtgtcca caattttacc agccttatga ttatgtaaaa aaatcaattg
      661 agcgaacaag ccgttgggca gaacgtggat taaatgccca ccgccgtcct catgatcaag
      721 gactatttgg tattgttcaa ggggcagggt ttgaggattt acgtcgtcaa tcagcacgag
      781 atttagtatc gatggatttt ccaggttatt caattggagg gcttgcggtc ggagaaacac
      841 atgatgagat gaatgcagtt cttgatttta ctgttccaat gcttccgaat gataagcctc
      901 gttatttgat gggagttggt gcaccagata gtttaataga cgccgttatc cgaggagtag
      961 atatgtttga ctgcgtctta cctactcgta ttgctcgaaa tgggacatgt atgacaagtc
     1021 aaggtcgttt agttgttaag aatgctaaat ttgcagaaga ttttacgcct cttgatccaa
     1081 attgtgattg ctatacttgt aaaaattata cacgcgctta tatccgtcac ttattgaaag
     1141 cagatgaaac ttttggtatt cgtttgacta gttaccataa tctttatttc cttgtcaatc
     1201 ttatgaaaga tgtcagacaa gctattatgg atgataatct tctagagttc cgtcaagatt
     1261 ttatggagcg ctatggttat ggcacgaata accgtaattt ttaaaaaagt tcctggtaaa
     1321 acagggcttt ttttgatctg tttgttaacc gcgttttaaa aaaagttgac aatagaaaaa
     1381 cttttgtata atatgttata ggaggaatca tgatgcaaga gtactttggt attggtcttg
     1441 atagtagcag ccgctgttac cactatcata ctaaattgga tattgtcgct ttaaaatgtg
     1501 ctgtttgtca aaaatattat gcttgttata aatgtcatga tgcactcgag gagcattgtt
     1561 ttgtagcaac aaaatctgat gaaatgtttc ctgtcttatg cggttcttgt cgtcagatgt
     1621 tgacattaaa agagtataaa actggttttt gtccttattg tcgtatgctt tttaatccaa
     1681 attgtcaacg ccatgattct atttattttt cgaaggagta atttttaatg tttcacacaa
     1741 aaagcctaat ccagatggct atgatggcta ctttattggt ggtacttggt ttcattcctg
     1801 ctctaccact tggtttcatt ccggtcccta tcgtcttaca aaacctaggg gttatgatgg
     1861 ctggtgttat tttgggcgga agaaaaggga gtttatctat tttgctcttc tttcttgtcg
     1921 gtttagttat tcctgtattc tcaggtttac gttcgactat acctgtattg acaggaccaa
     1981 cagctggtta tgttattgct tggttgtttg ttcctttgct tatctcatat ggtgtaaaat
     2041 tagtaaataa aaagaatttt atagctattt tccttattgt atggctagca ggggtccttt
     2101 ttgtagatgt agcaggagca gtttggctgg catcttatat gaacatccca ttagataaat
     2161 cacttcttca aaacttagta tttctcccag gagatacaat aaaagccatt atagctgctg
     2221 ttgttgctgt taaatataaa gatagttttt tcttgactga aaaataatca agattgctat
     2281 attttagatg gactagtaga tctaaaaatt tagatttact agtctttttt gtgcataaaa
     2341 tctaaaacag ttattcttaa caaaatttgg tataatagca ttaaatcagt aaggagggga
     2401 ttatcatgaa attaacaacg ttaggttgtt ggggtggtta tccttataaa gatggaggaa
     2461 ccacaagttt tctattaact ggagaaaata attttcactt attaatagat gcgggaagtc
     2521 gttcaatcaa tgagttagaa aaagaaataa gcccgttgga tttggatgca gttatcatca
     2581 gtcattatca tccagatcat gtagcagatt tgggtgtgtt gtatcattat cgtttgctat
     2641 accctaaaga taaatgggaa cccaaagtat tgccagttta tggacataat gaggatgatt
     2701 atgagttttc taaattgaca ttacctaacg ttgctcaagg aattgcttat cataccaatg
     2761 gagttgagca agttggtccc ttcaatatta cgtttatcaa aacagtccac ccagtcgtct
     2821 gctatgccat gaggattaca gaaagagaaa ctggtcaagt attagtcttt acaggagatt
     2881 ctgggtattt tgctgacctt gtacctttta caaaaggcgc agatttattt ttagcagacg
     2941 tttatcttta cgagggaaat gaaaatcata ttgcacattt gactagtaaa gaagcaggtg
     3001 agattgctta taacgctaaa gttaagaaat tagttttaac acatgttccg cagtttccgg
     3061 gagaaggaat aaaccccgaa aatcatttgg aagaacttcg actacagtct gaaaagtatg
     3121 caggtggcat ctctgttgat ttagctcagc cacataaatc ttgggactta ggaaaactat
     3181 aatgcagaaa tttacccaag aagaaaaaga atggtttatg acagaagctc taaaggaggc
     3241 aaaatcctcc ttagagaaag aagaaattcc tataggttgt gtcattgtaa aagatggtca
     3301 tattattgga agaggacata atgctcgtga agagtttaat aaagctattt tacatgctga
     3361 aatcatggct attaacaatg caaatgagaa ggtagggaat tggcgcttat tagatactac
     3421 tttatttgtt actgtagaac cttgtgtaat gtgtagtgga gctattggct tagctagaat
     3481 tccccacgtt atatatggcg ctaagaatac gaaatttgga gcagcgggaa gtctctatga
     3541 tattttaagt gatagccgac ttaatcatag agttgaagtt gaaaccggca ttttagaaga
     3601 tcaatgcgct aaaataatgc aggatttctt tcgaaaagga cgacaacgca agaaagaagc
     3661 taaactaagt gaaaaaacta agatttgata gcttgcaata aaatattagt tttgttataa
     3721 tatctttgga gcaacattta ctcgtgaagt gggtcagggg aggaatccag cagccctaag
     3781 cgatgctaaa tgtgtgctct ttttattttc aaaagtcaaa gtgtttttgg aaataattat
     3841 gaaagtttca taaaaattgg tagcgtttca aaaaatatga aaatacagtt aaaaaacctt
     3901 gaaaaaaaac tcacaaaaga gtaagatgat agtgaaacat aattgttagg agaaataaaa
     3961 tgacacatat tacatttgac tattcaaaag tcttgggtca atttgtaggc gaacacgagt
     4021 tagactacct acaaccacaa gtaagtgcag cagatgcttt ccttcgtcaa gggactggtc
     4081 ctggatcaga ttttctcgga tggatggacc tcccagaaaa ctatgacaaa gaagaatttt
     4141 ctcgcattca aaaagccgct gaaaagatta aatcagatag cgaagtactc gtggttattg
     4201 gtattggtgg ttcgtacctt ggtgcaaaag cagcaattga ctttttgaat aatcattttg
     4261 ctaatttgca aaccgcagaa gaacgtaaag cgcctcagat tctttatgct ggaaattcta
     4321 tttcatctac ttaccttgcc gatttagttg aatacgtcca agataaagaa ttctcagtaa
     4381 atgtcatttc aaaatcaggt acaacaactg aaccagcgat tgctttccgt gtatttaaag
     4441 aacttctagt taaaaagtac ggtcaagaag aagctaataa acgtatctac gctacaactg
     4501 ataaagttaa aggtgccgta aaagttgaag cagatgctaa caattgggaa acatttgtag
     4561 ttcctgataa tgttggtggt cgtttctcag tactgacagc tgtaggtctt cttccaattg
     4621 ctgcttcagg agcagatatt actgctctta tggaaggcgc aaatgctgct cgtaaagatt
     4681 tatcttcaga taaaatctct gaaaacattg cttatcagta cgctgcagtt cgtaatgttc
     4741 tttatcgcaa aggttatatc acagagattt tagctaacta cgaaccatca ttacaatact
     4801 ttggtgaatg gtggaaacaa ttagcaggtg aatcagaagg taaagatcaa aaaggtatct
     4861 atcctacatc agcaaacttc tcaactgatc ttcactcact tggtcaattt attcaagaag
     4921 gttaccgtaa cctctttgag acagttgttc gcgttgagaa acctcgtaaa aatgtgacta
     4981 ttcctgaatt aaccgaagat ttagatggtc ttggttatct tcaaggaaaa gatgtagatt
     5041 ttgttaataa aaaagcaaca gatggtgtgc ttcttgctca tacagatggt ggggttccaa
     5101 atatgtttgt aacgcttcct acacaagacg cttacactct tggttacact atttacttct
     5161 ttgagttagc aattggcctt tcaggttatc ttaactcagt aaatccattt gatcaaccgg
     5221 gggtagaagc atataaacgt aatatgtttg ctcttctagg taaacctgga ttcgaagagc
     5281 ttagcgctga attgaatgca cgtctttaaa aatatctaac taaaaagttc ctcaaagaga
     5341 ggaacttttt agttttgttg taggataact aaaaacccac tagcactggt agcgatagtg
     5401 ggttggaaaa agggttgaga aaagttatta acttttctca ggagatttat gaaaaaggaa
     5461 aataatttag gataatattt tcctaacggc taggataaac cgcttaattt taggatgatg
     5521 ctagtataca gtagaaacct taaataaacc ttaaaaattc atactttatc attttatttt
     5581 cccctat
//
LOCUS       DATRQA010000121         5577 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.121, whole genome shotgun sequence.
ACCESSION   DATRQA010000121 DATRQA010000000
VERSION     DATRQA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.121"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..799
                     /locus_tag="VBD27_001666"
     CDS             <1..799
                     /locus_tag="VBD27_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEO7025662.1"
                     /translation="MIAFQTSKSLKKHIDFLFEKGSMYLTYNDNLLFHGCIPMHSNGD
                     FKSFKIAGKTYGGRDLLDLFESQIRLAYARPEKHDDLATDIVWYLWCGENSSLFGKNA
                     MTTFERYYVSDKVTHQERKNPYFKLRDKDDICTALLQEFDLPKFGHIVNGHTPVKEKN
                     GEQPIKANGKMLVIDGGFAKGYQKNTGLAGYTLIYNSYGIQLISHLPFTSIEEVLSGT
                     NYIIDTKRLVEEAKDRILVKDTTIGQKLTKEIKDLDHLYRHFQEYDD"
     gene            889..2013
                     /gene="queG"
                     /locus_tag="VBD27_001667"
     CDS             889..2013
                     /gene="queG"
                     /locus_tag="VBD27_001667"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO7025663.1"
                     /translation="MDIKAEIQKLAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPNSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPLATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILEFMSNLTLKDEDSRKELELIRHKWQF"
     gene            2082..3180
                     /gene="prfB"
                     /locus_tag="VBD27_001668"
     CDS             join(2082..2153,2155..3180)
                     /gene="prfB"
                     /locus_tag="VBD27_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO7025664.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            3199..3891
                     /gene="ftsE"
                     /locus_tag="VBD27_001669"
     CDS             3199..3891
                     /gene="ftsE"
                     /locus_tag="VBD27_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO7025665.1"
                     /translation="MALIEMSGVTKKYRRSTTALRNLNLSIQQGEFVYLVGPSGAGKS
                     SLIRLLYREEKLSSGRLKVGEFNLNKLKRRQIPILRRSIGVVFQDYKLLPTKTVYENV
                     AFAMQVIGAKRRHIKKRVPEVLELVGLKHKMRSFPTQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEIAWEIMHLLERINLQGTTVLMATHNSQIVNTLRHRVIEIEAGSVI
                     RDEEKGEYGYHD"
     gene            3875..4804
                     /locus_tag="VBD27_001670"
     CDS             3875..4804
                     /locus_tag="VBD27_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025666.1"
                     /translation="MVIMINFFRHFWESLKNLKRNFWMTFASVTSVTITLLLVGLFSS
                     VLLNVEKLTTDVSGNFTISAFLNVDSTDAQKQVKDKDGKLKDNPDYHKVYDKIKRISG
                     VEKVTYSSKAEQLKEVQKEYGSDVIDDTYKDALLDVYVVGTSSAKVSKSVSEAIGRIE
                     GVDYTKEPIDSTKLSNLTDNIRIWGFGGVALLIVLAIFLISNTIRMSIMSRRTDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGILGAIVPSLIFYFGYQFVFNKFNPKFETSHVSLYPM
                     DIMVPAIIGGMVIIGIIIGSLGSVLSMRRYLKI"
     gene            4878..5577
                     /locus_tag="VBD27_001671"
                     /pseudo
     CDS             4878..5577
                     /locus_tag="VBD27_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_088186184.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS861 family transposase"
BASE COUNT         1864 a          848 c         1156 g         1709 t
ORIGIN      
        1 gatgatagcc ttccagactt ctaaaagttt aaagaaacat attgatttcc tatttgagaa
       61 aggaagtatg tatttaacct ataatgataa tcttttattc catggttgca taccaatgca
      121 tagtaatggt gattttaaaa gctttaaaat tgcaggaaaa acttatggtg gtagggattt
      181 actggattta tttgaaagtc aaatacgtct agcatatgct agaccagaga aacatgatga
      241 tttggcgaca gatatcgttt ggtatctttg gtgtggtgaa aattcttctc tttttggtaa
      301 gaatgccatg actacttttg agcgctatta tgtttccgac aaagtaactc atcaagaaag
      361 aaaaaatccc tactttaaac tgagagataa agatgatatt tgcactgccc ttttacaaga
      421 gtttgatttg cctaagtttg gacatattgt caatggtcat acgccagtca aagaaaaaaa
      481 tggtgagcaa ccgataaaag caaatggtaa gatgttagta attgatggtg gctttgccaa
      541 aggttatcag aaaaatacag gtttagctgg ctacactttg atttataata gctatgggat
      601 acaattaatt tcacaccttc cttttacatc cattgaggaa gttttaagtg gaaccaacta
      661 tatcattgat acgaagcgat tagtagaaga agccaaggat aggattcttg taaaagatac
      721 aactattgga caaaagttaa ccaaagagat taaagactta gatcaccttt atcgtcattt
      781 tcaggaatat gatgactagt gtttaagtaa tttactaatt gatatgtagg atttaatttt
      841 tcagtttctt accttgtctc ttttattaaa ttgacttaaa ataatattat ggacataaag
      901 gcagaaattc aaaaattagc aaaagaaatt ggtatctcta aaattggttt tacgacggca
      961 gataactttg attatttgga aaagtcattg cgtgcttctg tggaagaggg gcgcaattca
     1021 ggatttgaac ataaagtcat agaggatcgt atctaccctg aaaggctttt agaatcggct
     1081 aaaacgatta tttcaattgg cgttgcttat cctcacaaac ttcctcagca acctcaaaaa
     1141 acaccttata aacgaggaaa aattacacct aattcgtggg ggcttgatta tcattatgtg
     1201 gtcggcgaaa agttagacag actatcaaaa ggcattgaag agctctgtcg agattttcct
     1261 ttgcaacaaa aagcaatggt cgatactgga gctttagttg atactgcagt tgcccaaaga
     1321 gcaggtattg gttttatagg aaaaaatggt ctagttattt ctaaagaata tggctcttat
     1381 atgtttttgg gagaattaat taccaattta gagatcgagc cagacaagcc tgtcgattat
     1441 gattgtggtg actgtcgtcg ttgtctagat gcctgtccaa cttcttgttt aattggtgat
     1501 ggctcaatga atgccaagcg ttgtctatca tttcaaaccc aagataaggg aatgatggac
     1561 atagagtttc gaaaaaagat taaaacagtt atctatggtt gcgatatttg ccaaatttgc
     1621 tgtccttata acaaaggtat aaataatcca ttggcgacag agattgatcc tgagttggct
     1681 caaccagaat taataccatt tttgagttta tcaaatggac aatttaaaga aaaatttggt
     1741 atgattgctg gaagttggcg aggtaaaaat attttacagc gtaatgccat tattgcgcta
     1801 gcaaatgctc atgataaaac cgcagtggtt aaattaatag agattattga taaaaataat
     1861 aaccctattc atactgcaac agctatatgg gcattgggag aaattgtcaa aaaaccaaac
     1921 gatgagatac tggagtttat gagtaattta accttaaaag atgaagatag ccgaaaagag
     1981 ttggaattaa tccgtcataa atggcaattt taagcctttt atggtatttt taagaacctt
     2041 atgataaaat aaaacagatt aacaaagatt ggagaaatct catggaagtg gctgaaattc
     2101 gccaaaaaat agtagaaaat aaagagaagt tgactagctt caggaggtct ctttgactta
     2161 gatcgtttgg aagaggagat tgctcttctt gaaaatcaaa tgactgagcc agacttttgg
     2221 aatgacaata ttgcagcgca aaagacatct caagaactga atgagttaaa aggtaagtat
     2281 gatactttcc ataatatgca agaattatct gacgaaacag aactcttact tgagatgctt
     2341 gacgaagatg attctttgaa agaagaatta gaagaaaatc taatgcagct agataagata
     2401 atgggtgctt atgaaatgac gcttctctta tctgagcctt atgaccacaa taatgctatc
     2461 ttagaaatcc atcctggttc aggaggaact gaagcgcaag attggggaga tttactctta
     2521 cgaatgtata cccgttttgg caatgctaat ggtttcaaag tagaagtatt ggattatcaa
     2581 gcaggggatg aagctggtat taaatcggtt accctctctt ttgaaggtcc caatgcttat
     2641 ggtttgttga aatcagaaat gggagttcat cgcctcgttc gtatatcacc ttttgattcg
     2701 gctaaacgtc gtcatacctc atttgcttca gttgaagtga tgccagagtt agatgatacg
     2761 attgaagtag aagtacgtga tgatgacatt aaaatggata ctttccgttc cggaggagct
     2821 ggtggtcaga acgtcaataa agtatcaaca ggtgttcgtt tgacgcatat tccaactgga
     2881 attgtggtat cgtctaccgt tgaccgtact caatatggga accgcgatcg cgctatgaag
     2941 atgttgcagg ctaagctata tcagttagaa caagaaaaga aagcacaaga agtagatgct
     3001 ctcaagggtg ataaaaaaga aatcacttgg ggaagtcaga ttcgttctta tgttttcacc
     3061 ccttatacga tggtgaagga tcatcgtact aactttgagc ttgctcaggt agataaggtt
     3121 atggatggag aaatcaatgg ctttattgat gcctacctta agtggcgcat tgaagactaa
     3181 aaataatgaa ggagaaacat ggcactaata gaaatgtctg gtgttactaa aaagtatcgc
     3241 cgttcaacaa ctgccttgag aaacttaaat ttatcaattc agcaaggtga gtttgtgtat
     3301 ttagttggtc cgtcaggtgc tggtaaatct agcttgattc gcctcctata ccgtgaagaa
     3361 aaattaagtt caggtagatt aaaggtaggg gaattcaatc tcaataaact caaacgtcgt
     3421 caaattccta ttttacgtcg tagtattgga gttgtcttcc aagattataa attactacca
     3481 acaaaaacag tttatgagaa tgttgcgttt gcgatgcaag ttattggagc taagcgccgt
     3541 catattaaaa aacgcgtgcc agaagtttta gaattagtag gtttaaaaca caagatgcgt
     3601 tcttttccaa cacagttatc tggtggagaa caacaacgtg tggcaattgc acgtgcgatt
     3661 gttaataatc caaaattatt aattgcagat gaacctactg gtaatttgga tccagaaatc
     3721 gcttgggaaa tcatgcatct attggaacgt attaaccttc aaggaacgac tgttttgatg
     3781 gcaacacaca atagtcaaat tgttaacaca cttcgtcatc gtgtcattga gattgaagca
     3841 ggtagcgtta tccgagatga agagaaggga gaatatggtt atcatgatta atttctttag
     3901 acatttctgg gaatcgctta aaaacttaaa acgtaacttt tggatgactt ttgcttctgt
     3961 aacatcagtt accataacat tacttcttgt aggcttgttt tcatcagttt tactgaatgt
     4021 tgaaaagtta acaactgatg tttcaggtaa ttttactatc agtgcctttt taaatgttga
     4081 ctcaacggat gctcaaaaac aagtcaaaga taaggacgga aagctaaaag ataatcctga
     4141 ttatcataaa gtttatgaca agattaaacg tatttctggt gtagagaaag tcacttattc
     4201 aagtaaagct gaacaattaa aagaagttca aaaagagtat ggttcagatg ttattgatga
     4261 tacctacaaa gatgctttgt tagatgttta cgtagttggg acaagttctg caaaagtttc
     4321 taagagtgtc agtgaagcaa ttggacgcat tgaaggtgtt gattatacta aagaacctat
     4381 tgattcaaca aaactatcta atctcacaga taacatccgt atatggggat ttggtggtgt
     4441 tgccttatta attgtccttg ctatcttttt gatttcaaat actattcgta tgtcgattat
     4501 gtcacgccgt acagatattg aaatcatgag attagttggc gctaaaaatt cttatattcg
     4561 aggaccgttc ttttttgaag gtgcttgggt agggatttta ggggctatcg ttccatcgct
     4621 gattttctat tttggttatc aatttgtgtt taacaagttt aatccaaaat ttgagacaag
     4681 ccatgtttca ttgtatccta tggatattat ggttccagca attattggtg gtatggtgat
     4741 tattggtatt attattggct cacttggatc tgtcctatca atgagacgtt accttaagat
     4801 ttaataaaaa gagatgaaat ctatgaactg caccccaaaa gttagacaaa aaatctaacg
     4861 attggggtgc ttttctgatg aaattaagtt atgaagataa actagaaata tacgagttaa
     4921 gaaagatcgg gatgtcctgg tctcagatta gtcaacgata tgatgttcgt atctcgaatc
     4981 ttaaatacat gataaaactc atggatcggt atggtgtaga aatcgtcgaa aaaggtagaa
     5041 atgagtatta tccacctgaa ttaaagcagg aaatgataga taaagtcttg attcatggtt
     5101 gttctcaact ctctgtttct cttgattatg ctctctctaa ttgttctata ctcacaaatt
     5161 ggctttccca atttaagaaa aatggttata ctattgttga gaaaacaaga gggagaccga
     5221 gtaaaatggg acgtaagcgt aagaaaactt gggaagaaat gacagagctc gagcgacttc
     5281 aggaagaaaa tgagcgcttg agaacggaga atgcctttct aaaaaagttg agggatttgc
     5341 gcttgaggga cgaagcctta cagagcgaac ggcagaaaca attagagaaa tggtcacagg
     5401 aggattccga ctagacctct tacttgagat aactaagata gctcgtgcaa cttactatta
     5461 tcaactaaag aaactgaata aaccagataa agacaaagca atcaaatctg acattcaatc
     5521 catttatgat gaacatagag gaaactatgg ctatcgtcgg atttatttag aactgcg
//
LOCUS       DATRQA010000122         5468 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.122, whole genome shotgun sequence.
ACCESSION   DATRQA010000122 DATRQA010000000
VERSION     DATRQA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.122"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            212..1378
                     /locus_tag="VBD27_001672"
     CDS             212..1378
                     /locus_tag="VBD27_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7025667.1"
                     /translation="MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYN
                     WELSGNVSSYGNSMKNGADLAVKEINAAGGVGGKKLKVLSQDNKSENAEAATVATNLV
                     TKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQYFFRATF
                     VDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGD
                     TDFQASLTKLKGKEYDAIVMPGYYTETGLIVKQARDLGISKPVLGPDGFDSPKFVQSA
                     TPVGASNVYYLTGFTTQGSTKAKAFHDHYVKAYGEEPSMFSALSYDAVYMAAKSAKGA
                     KTSIDIKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLEDGKTSSVNIISAK"
     gene            1484..2353
                     /locus_tag="VBD27_001673"
     CDS             1484..2353
                     /locus_tag="VBD27_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO7025668.1"
                     /translation="MLQQLVNGLILGSIYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FMGYYLINHLHLNFFLALLIAMLGSAFLGVVIEYLAYRPLRKSTRIAALITAIGVSFL
                     LEYGMVYLVGADTRAFPQAIHTVKYNLGPITITNVQLIILGIALLLMLTLQFIVQKTK
                     MGKAMRALSVDSDAAQLMGINVNRTISFTFALGSALAGAGGVLIGLYYNSVQPLMGVT
                     PGLKAFVAAVLGGIGIIPGAAIGGFVIGILETLATALGVSDFRDGIVYAILILIFLIR
                     PAGILGKNIKEKV"
     gene            2356..3309
                     /locus_tag="VBD27_001674"
     CDS             2356..3309
                     /locus_tag="VBD27_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO7025669.1"
                     /translation="MKENMKSILSWLAIVVLGYLLISVLISMGIFNLYHIQIIETIGI
                     NVILAVGLNLIVGCSGQFSLGHAGFMAIGAYAVAIIGVKMPTYVGFLIAILVGTLVAG
                     GIALGVGIPTLRLKGDYLAIATLGVAEIIRILLVNGGDITNGAAGIMGIPPFTTWSLV
                     YGVAVVSLILAMNFLRSPLGRNTIAIREDEIAAESMGVDTTKVKVIVFVFGAILASIA
                     GSLQAGYVGTVMPKDFSFMMSVNVLIIVVLGGLGSMTGTVLAAILLGLLNMLLQDYAS
                     VRMIIYALALILIMIFRPSGLLGTKELTLSHLFRKTSKGGN"
     gene            3310..4074
                     /locus_tag="VBD27_001675"
     CDS             3310..4074
                     /locus_tag="VBD27_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025670.1"
                     /translation="MALLEVKNLSKHFGGLTAVGDVSMKLHKGELIGLIGPNGAGKTT
                     LFNLLTGVYLPSKGTISLDGKILNGRKPAKIASLGLGRTFQNIRLFKNMTVLDNVLVG
                     LSNHHLSHPIASFLRLPKYYHSEKALRKKALELLEIFGLKAYQDALAKNLPYGKQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLISQIKSDFDITIMLIEHDMNLVMQV
                     TERIYVLEYGRLIAHGTPEEIKNNKRVIEAYLGGEL"
     gene            4074..4784
                     /locus_tag="VBD27_001676"
     CDS             4074..4784
                     /locus_tag="VBD27_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025671.1"
                     /translation="MTMLKVENLSIHYGVIQAVNDVSFEVNQGEVVTLIGANGAGKTS
                     ILRTISGLVRPSQGSISFMGKPIHKLAARKIVGNGLAQVPEGRHVFSSLSVMENLEMG
                     AFLQKDREQNQKMLKKVFDRFPRLEERKNQDAATLSGGEQQMLAMGRALMSRPKLLLL
                     DEQSMGLAPIFIQEIFNIIEDIKKQGTTVLLVEQNANKALTIADKAYVLETGKVVLSG
                     TGKELLVSDQVRKAYLGG"
     gene            4803..5462
                     /locus_tag="VBD27_001677"
     CDS             4803..5462
                     /locus_tag="VBD27_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7025672.1"
                     /translation="MPVKDFMTKKLVYVSPDTTVAEAADLLREHHLRRLPVVENDQLV
                     GLVTEGTMAEAQPSKATSLSIYEMNYLLNKTKIRDIMIKDIVTVSQYASLEDAIYLMM
                     SRKIGVLPVVDNGQLYGIVTDRDVFKAFLEIAGYGQEGYRLVILADEGIGVLSKVLNR
                     LSSANLSVKRLVIIERKAGKKAVEIQLEGYADKDVLKQELVFDDVIVETLEKTMKKPL
                     S"
BASE COUNT         1700 a          805 c         1182 g         1781 t
ORIGIN      
        1 cggttatcag caattgatga tattgatcag gattttgttg gaaacttata tcgttaagat
       61 atttactagt agttattaga aagaaggagt tcagatgtcc ttctttttta tttagtttct
      121 ataattttca aaaaaacaat ttaaaaacta ttgacaatat tctgataatt ctgtattatt
      181 ttagttacca ttttcaaaaa aggagaaaaa tatggaaaag agacttagtt taggagcgct
      241 tgttttagct agtacagttc ttttagcagc atgtggaaat gtcggaggag gagcatcttc
      301 gacaggaaca aaaataggga aagatattaa ggtagggtat aactgggaat tatcaggaaa
      361 cgtttcttca tatggaaatt caatgaagaa tggagctgac ttagcagtaa aagaaattaa
      421 tgctgctgga ggggttggcg gcaagaagtt aaaagtccta tcacaggata ataaatcaga
      481 aaatgcagag gcagcaacgg ttgccacaaa cttggttacc aaaggagcta atgtcattat
      541 cggaccagca acatcgggtg cagctgcatc ttcaactcca aaagtaaatg cagcagcagt
      601 tccaatgatt gcacctgctg cgacacaaga caatttagtc tatggttctg atggaaaaac
      661 cttaaatcag tatttcttcc gagctacttt tgtcgataat tatcaaggaa agctattgtc
      721 tcagtatgct acagacaacc ttaaagctaa aaaagttgtt ctattttatg ataattcatc
      781 agattactca aagggggtag caaaatcatt taaggaaagt tatagtggaa aaattgttga
      841 tagtatgaca ttctccgctg gtgatactga tttccaagcg tcattgacta agttgaaagg
      901 gaaagaatat gatgctattg tgatgccagg ttactatacc gagacaggat taatagttaa
      961 gcaagcgcgt gatttaggta tctctaaacc ggttcttggg cctgatggtt ttgatagtcc
     1021 gaaatttgtg caatcggcaa cacctgtagg agcttcaaac gtttattatt tgacaggttt
     1081 cactacacaa ggatcaacca aagctaaagc tttccatgat cattacgtta aggcatatgg
     1141 tgaagaacca tccatgttct cggctttatc atacgacgct gtgtatatgg cagctaaatc
     1201 tgctaaagga gctaaaactt caattgatat taaaaaagct ttagctaagc tgaaagattt
     1261 taaaggtgtg acagggaaaa tgtctattga taaaaatcac aatgttgtta aatcagctta
     1321 cgttgtcaaa ttagaggatg gaaaaacaag tagcgttaac attatttcag caaaataagt
     1381 attctctaga aaaatgggag tttaattttc agctcccctt ttttaaaaag taatttttca
     1441 gaataattcg acaataatat ataatagaaa gtatggttat aatatgcttc aacagctcgt
     1501 taatggactt atcttaggat ccatatatgc tttacttgcc ttaggatata ctatggtata
     1561 tggcattatt aagctgatta attttgctca tggggacatc tatatgatgg gcgctttcat
     1621 gggctattat ttgattaatc atcttcatct taattttttc ctagctttat taattgcaat
     1681 gctggggtct gctttcctag gtgttgtaat tgaatattta gcttatcgtc cactgagaaa
     1741 atcaactaga attgccgcgt taattacagc tattggtgtt tcttttcttt tggagtacgg
     1801 gatggtatac cttgttggag cggatactag ggcatttccg caagccattc atactgttaa
     1861 atataattta ggaccgataa ctataaccaa tgttcagctc atcattttag gtattgcgct
     1921 attactaatg cttactttac agttcatcgt tcaaaaaact aaaatgggga aagctatgag
     1981 agctttatca gtggatagcg atgccgcaca attgatggga attaatgtta accgtaccat
     2041 tagttttaca tttgcgctgg ggtctgcttt agctggagca ggtggagtac tgattgggct
     2101 ttattataat tctgttcaac ctcttatggg ggtaactcca gggttgaaag catttgttgc
     2161 agctgtctta ggtggtattg gtatcattcc gggagcggct atcggtggtt ttgttattgg
     2221 cattttagaa actttagcga ctgcacttgg tgtgtcagat tttcgagatg gtatcgttta
     2281 tgctatttta attctcattt tcttgatccg tccagcagga attcttggaa aaaatattaa
     2341 ggagaaggtc taggcatgaa agaaaatatg aaatcaattt tatcttggtt agctatagta
     2401 gtgttaggtt atctcttgat tagcgtgcta atatctatgg gtatatttaa cctctatcat
     2461 attcaaatta ttgaaacaat tgggattaat gttattttag cagttggttt aaacctaatt
     2521 gtaggttgtt cgggacagtt ttcactagga catgcaggtt tcatggctat tggcgcttat
     2581 gcagttgcta ttatcggcgt taaaatgcca acttatgtag gttttttgat agctatactt
     2641 gttggaactc ttgttgcagg aggtattgcc ttaggtgtag gaattcctac tttgagacta
     2701 aaaggtgatt atttagctat agctacttta ggtgttgctg agattatccg tattttgttg
     2761 gttaatggag gtgatattac gaatggtgct gcgggtatca tggggattcc gccttttaca
     2821 acctggtctt tagtttatgg ggtggcagtt gtgagcctta tccttgcaat gaattttttg
     2881 cgtagtccct tagggcgtaa tacaattgct atacgagaag atgaaattgc agcagagtct
     2941 atgggtgtgg atacaacaaa agtgaaagta attgtttttg tttttggagc tattttagct
     3001 tctatcgctg gtagtttaca ggctggctac gttggaactg ttatgccaaa agacttttca
     3061 tttatgatgt ctgtcaatgt tttaattatt gttgttttag gtggtttagg ttcaatgaca
     3121 gggacagttt tagcagcaat tttattagga ttattgaata tgttactaca agactacgct
     3181 tctgttcgaa tgattatcta tgctttagcc ctgattttaa ttatgatttt tagacctagc
     3241 ggtttattag gaacgaaaga gttaactcta tctcatctat ttcgtaagac gagtaaggga
     3301 ggcaattaga tggcacttct tgaagtaaaa aatttaagta aacatttcgg tggcctaact
     3361 gctgtaggtg atgtttcgat gaaattacat aaaggtgaat taattggctt aatcggcccc
     3421 aacggtgcgg gtaagacaac gctttttaat ctcttaacag gagtatacct tcctagtaaa
     3481 gggacaatat cacttgatgg taagatatta aatgggagga aaccagctaa aatagcttct
     3541 ttaggtttag gtagaacttt tcaaaatatc cgacttttta aaaatatgac tgttttagac
     3601 aatgtactag taggactcag taatcatcac ttatcacatc ctattgctag ttttttacga
     3661 ttacccaaat actatcatag tgaaaaagcc ttaaggaaaa aggcactgga gctattagag
     3721 atttttggtt tgaaagcata tcaagatgct cttgctaaga acctccctta tggaaaacaa
     3781 cggcgtttag aaattgttcg tgctttagca acagaaccaa aaatattgtt tttggatgag
     3841 cccgcggcag gaatgaatcc tcaagaaact gcagaattga cgcagttaat tagtcaaata
     3901 aagagtgatt ttgatattac tattatgctg attgaacatg atatgaactt ggttatgcaa
     3961 gtaacagaga gaatttatgt attggaatat ggtcgtctga ttgctcacgg gacacctgaa
     4021 gaaattaaaa ataataaacg tgttatagaa gcatacttgg ggggggagct ataatgacca
     4081 tgttgaaagt agaaaatcta tctattcact atggtgttat tcaagcggtg aatgatgtct
     4141 cgtttgaagt taaccaaggt gaggttgtaa ccttaattgg agccaatggt gcaggaaaga
     4201 catcaatttt gaggacaatt tctggtttag taagaccaag tcagggttct atttctttta
     4261 tggggaaacc tatacataag ttagcagcta gaaaaattgt tggcaatggt ttagctcagg
     4321 ttccagaagg gcgccacgtt ttctcaagtt tgtctgttat ggaaaattta gaaatgggag
     4381 cctttcttca aaaggatcgt gaacaaaatc aaaaaatgtt aaaaaaagtg tttgatcgtt
     4441 ttcctcgctt agaagaacgt aaaaaccaag atgcagcaac cttatcaggt ggtgagcagc
     4501 aaatgctcgc tatggggcgt gcattgatga gtcgtcctaa attattactt ttagatgaac
     4561 agtcaatggg cctagctcca atttttattc aagaaatttt taatatcatt gaagacatca
     4621 aaaaacaagg gactacagtt cttttagttg aacaaaatgc aaataaagct ctgacgattg
     4681 ctgataaagc atatgttcta gagactggga aggttgtttt atcagggaca ggtaaggaat
     4741 tactagtttc agatcaagtt cgtaaagctt acttaggtgg ataatattta gaggagtcag
     4801 gtatgccagt gaaagatttt atgacaaaaa aattagttta tgtctctcca gatactacag
     4861 ttgcagaagc tgctgattta ttgagagaac accacttaag gcgtttgcca gttgttgaaa
     4921 atgatcagct agtggggtta gtgacagaag ggacaatggc agaagcacaa ccttctaaag
     4981 caaccagtct ttctatatat gagatgaact atcttcttaa taagacaaaa attcgagata
     5041 ttatgattaa agatattgtg actgtttcac agtatgctag ccttgaggac gctatttatt
     5101 tgatgatgtc tcgaaagatc ggtgttttac cagtagttga taatggacaa ctttatggga
     5161 ttgtcacgga tagggatgtt tttaaagcct tccttgaaat tgcggggtat ggtcaagaag
     5221 gttatcgctt ggttatatta gctgatgagg ggataggggt actatcaaaa gtcctaaatc
     5281 gtctttctag tgcaaattta agtgttaaac gtttagtcat tattgaacga aaagcaggta
     5341 agaaagcggt cgagatacaa ctagaaggat acgctgacaa ggatgtttta aaacaagaac
     5401 tagtatttga tgatgttatt gtggaaactt tagaaaagac catgaaaaaa ccactttctt
     5461 aaaaatat
//
LOCUS       DATRQA010000123         5459 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.123, whole genome shotgun sequence.
ACCESSION   DATRQA010000123 DATRQA010000000
VERSION     DATRQA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.123"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            106..5407
                     /locus_tag="VBD27_001678"
                     /pseudo
     CDS             106..5407
                     /locus_tag="VBD27_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822652.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT         2002 a          874 c         1038 g         1545 t
ORIGIN      
        1 aaatactcat taattagcaa atagatattg acaactctat acaaaagtgc ttaaattaag
       61 cttgcttaac aaataaaata atctaactta aaaatgagtg gaattatgaa aagaatttct
      121 ttaaaaacaa ccggtgtact gattgcgttt ttagctgtaa atgcggtagc gacctctgta
      181 gtggtagatg cagacacagt agtccctcat actagtgcga atactagtca gcttttagaa
      241 aaaaacacaa ctatcaagcc ttccgaagtt aaacagtcac gtactgaaag taatgcagta
      301 acccctccaa caatagcctc gagtaactca caaaagcttg agaataaatc atctgtagat
      361 tcaaatatac taaagagtga acaacctgca cataaagttg ctggtgttaa acaagtcgtt
      421 caagaacaaa cgaaaaatca aaaagatgaa acctctttac aggctaaaaa gcaagtacaa
      481 tcaagtgaaa agttattaaa tacttcatca caaccttcaa gtgaggaaaa caaaaacact
      541 aataagccta ataaggctaa tgaagtggat atgaaaaatg tcgtgaataa tgcttcgacg
      601 aagccaactc ccccatctga ttctaaagtg tataagcgcg ttattgtaac atttgatcaa
      661 gtaaataagg aaaaagtaga tgaagccctt aaagcaattc cgggcactcg tattaagtta
      721 acttatagta gcctttttaa tggctattca ttagcagtag atgaaaaaga aattgaaaaa
      781 attggtaaga ttaaagagat aaaacatatt gaagcagttc aagtattaaa acctcaaatg
      841 tttactgcta accaatggac acatgtctta gctgcacgtt taaaagctaa acaaaatagt
      901 aaagttgacg gtagagggct acttattgcg actattgata gtggtgtaga tatccatcat
      961 cctgccatgc gattggatga gaatgatcca gatgtgatgg caaagcgtcg tattaaagat
     1021 attaaggctc cttatacaaa taaagttcct tttgcataca attatcaacg aggcgataat
     1081 ttagaattga aagacgacgt tcagcgccct catggtatgc atttagcagg tatcttagcg
     1141 ggaaatgcaa aggatggttt taaaggagtt gcccctaatg cacagttatt agtgtacaaa
     1201 atttcaacaa atagagaaga tccatctgca gagtggaccg gctctgatgc agtcttccat
     1261 gcaatggaag atgccgctaa aagaggggca gacgttatta gtttaagctt tggatatttg
     1321 ggaacaggac gtccaggtga actttggtat gagacaatta agaaggtgac tgacaagggt
     1381 gttatcgtag tagcagcaat gggaaactat ggtaattcgg cttcatctac tacctacgat
     1441 aatgtcgttg acaactcttt gggaacaaaa gatactgctg ctatggtgtc tgttgcagct
     1501 gttgataagg ttattggagt tggttcagtt caaaatagtg ctcttctttt agatgttttg
     1561 aaagttggaa atcttaaatt aggatatgca gaactgggac ataaaagtca aacaatatta
     1621 aaaatgctca aaaataaaac atttgatcta gtctatgttg gtaaaggcca gttgaaaaaa
     1681 gatatctcag aagagtacct taaaagttat actggaaaag ttatcgtcgt tgagcgtgga
     1741 ggagagggcg ttaaagataa gctaaaacgt tttatcggaa ttgctaaagg ggttattatg
     1801 ataaatgaac ctgcttatta tagttctggg aattttgata ctcaccccat gtttgattat
     1861 cactatgata taccagaagg taaatgggct attagtctat caaaaaaaga tggtgatcag
     1921 ttgattgact acatcaagaa aaacaaaact gctaaattag aatttttacc taaagaaacc
     1981 tttgttcgcc gtccaaatcc tacaatttca ggcttttcta gttggggagt gacaccagat
     2041 ttgcatttga agccagatgt agttgctcca ggagaagata tttattctac ttttaacgac
     2101 ggtacctatg ggtacatgtc tggaacttca atggctaccc ctcaagtagc tgccattagt
     2161 gcattagtaa aaagtaaagt agctgaaata atggcattaa aacttccaat gttagctggt
     2221 ttgacaaacg ttgatttgac aaagattgtc ttgatgaata caagtcaacc tttgaaagat
     2281 actctaaata aagctttaga aatttcacca cgacgtcaag gtgcaggtat ggttaactta
     2341 gaaaaagctt tagctaacga tgttcttgtt tttggtgata aaacaggatc attctctata
     2401 aaagagatta agaaccataa aagatttacc ttgaaattgg tcaatacagg acaaaaacgt
     2461 cgtaacttta gtattgaacc aggagcgatt ttaacaacta aaattgttga taaagtgact
     2521 aatcaggggt atgaaaatgt cgttttaaag gcagttcacg ctaaagtaat agagggagca
     2581 actttacaaa tgggtaaagt tatctcacta gcaccgcaat cagccataga gcttgatata
     2641 gatcttgaga taaaaaaacc tatacaagat tttgctgaag ggtatttgta tttcaaaact
     2701 ttagataaag atggacaaga tatctcagct ccttacatgg gcttctcagg aacatgggat
     2761 taagaaccaa tttttgacgc acctatgtgg gatagtcgtt ctaaaaataa attaacgacc
     2821 ttgatgcaag caactttggt tggacacgga actaacaaat tttatgaaat cggacaagaa
     2881 ataggtagta aaaaggtcaa tccatcccta tttactatgt ctaatgtgcg tgtagcacct
     2941 cgattttctt tattacgctc agcatcagat tttgacctct ctattgtatc agaagcaaaa
     3001 gacgattcac caatacttcg tcgtataaca gtgaaacatt tcgtggagaa atattacaaa
     3061 tcaagaaatg aggaagaaga ctcatattat gatgctttga gaacgacaga atcatcgcta
     3121 gtttgggatg gaaagtttta tgatcctaag actggtgatt ttaaagctgc aaagccaggc
     3181 ttatattact ttaagttacg tgcacgttca aatcctacag atccttatca agtccaatac
     3241 cttccaattt taatagataa tgaaagacca actttttctc ttaaaaaagt taataacggc
     3301 tttgatattt taacccatga taatcatcgt gttaaagaag taattgttcg taataaagag
     3361 acacaaaagc ctatcacagt taaaaaaatt gccgaaaatc actatcatat tgatgagtta
     3421 acgttaagag aatctgatct ggatataaaa gttgcagact atgctgggaa ttcaaacaag
     3481 gaaattgctt tatcagaatc agaagaagta tcagagaaat cttactctag tgaggaagat
     3541 aatacattac aaccacagtc actgtctcat cgcaatatga aagctcaagc aaccagttca
     3601 gatgatgacg atatctggga tgataacgat gatgacctta acgaagaaaa agattttgat
     3661 tttgaagaaa atatttctga tatcgaggat ggtttattat taactcgaga aacggtatat
     3721 tcaattcgaa ctgtaaataa aattcgaaat gttgaaaatg aaagcttgga gcatagtgtt
     3781 tctctcttat taaaagatgg ctatcgtgca gaaataagag ttgttaacct tcatgagaac
     3841 gctaaagatc caaaacattc cgacccatat cgtacagtat ccaaccctat ttatatgtat
     3901 ggtgatgact ctgacacatt taaaaccaca tgggtcaaat ttatgtctgg gaataacatc
     3961 atgaatttga aggtttataa taagaatgat aaattggtat ttagtcgtgg ctatcatatt
     4021 accgttgatg tacaacctcc aaaattagaa ctggacaaga ctaacataga agaagctgct
     4081 gacaaagata actttatcac tggtcaaatc tatgctcaaa atggagaaat tgaaatcaaa
     4141 gggcacgttt ctgataatgt aaaaggttgg ggtctttcta ttaatggtga tagcgtcgat
     4201 gttaaatatt caagtggtga atttatcagt aatagatgag catttactca taagataaaa
     4261 gcagcagcag gagatattgt tacgatttca gcggtagata catcaggtaa taccgtttct
     4321 gaacattata aggtagttaa tgatgtggat acaccgaagt taactattaa tgaagcagat
     4381 ttattatccg gaaaagtcaa agtggttata gctaaagcag aagataatca ccttaaaaat
     4441 ttaacgataa ctgttaatgg caagccctac acagaaggac aaagtattga ctcttaccaa
     4501 ttaaaaggtt tcgaagggcg ttacgtcatc aaagtatcag cagttgataa atcaggtaaa
     4561 gtcactgaaa aaattataac aatcggcaac ttccctgaat tatcagcaag tttacgcaaa
     4621 acctctttcc aaatgcaaga tcttacctct atcgataatt tattatccct agcccacaat
     4681 cagactgcgg aattgattaa gccagtccaa ctcatggaca aaaaagtgag ctttacggca
     4741 aaaatatctg acccccttgg tcgttctgtg gttagagagt ttgaagcatt attatcaact
     4801 tcaaacctta aagtagattc aaaactaaaa ccacatttga aaaaacaaac tctcctttta
     4861 acagatccaa aaggaattac ggaccttttg gatctgttaa aaggagagct ctattttatt
     4921 gacagaaata gtcgataata aagtgggaaa tcatactata aagggtttga tatttaaaaa
     4981 tggtgcatat aaccatttcg aaacaagtat acaaattgta aaaacaatgg aagaaatcaa
     5041 ctctgacaga cgtttgaaaa atgatcataa aagctctgac gacacaaaac taaaaaaaga
     5101 aacagagtcg ttaaaagggc ataaggttga tccagcagtt catggtgaat ttaaagaaat
     5161 atctaaacat caaatggata aaccacaagt gatagctgta ggcatgaaag ggagtacaaa
     5221 gacaccccca tttgttcata gtcaaatgga agcgaagaaa acaactaaga ttccaatgtc
     5281 tagagcggca aaacatgaat tgccaaggac aagtgatgac aataataatt tactggctaa
     5341 aggaattgca ctcatagtaa ttgtacttgg tttactaggc cttaaaaaag cttcaaagaa
     5401 atattgattt tgattaaatt agttttctaa tttattaagc taaaattcct ttggataaa
//
LOCUS       DATRQA010000124         5413 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.124, whole genome shotgun sequence.
ACCESSION   DATRQA010000124 DATRQA010000000
VERSION     DATRQA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.124"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            112..501
                     /locus_tag="VBD27_001679"
     CDS             112..501
                     /locus_tag="VBD27_001679"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO7025673.1"
                     /translation="MGNIELKKLAVKASENAYVPYSKFPVGAALRTAEGKIFTGCNVE
                     NISYGLANCAERTAIFKAVSEGYKDFSEIAIYGNTEEPISPCGACRQVMVEFFNKNAK
                     VTLIAKNGKTVETTVGELLPYSFVDLK"
     gene            566..1615
                     /locus_tag="VBD27_001680"
     CDS             566..1615
                     /locus_tag="VBD27_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO7025674.1"
                     /translation="MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDT
                     GGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDYFQSASESDYATNLDTAVSSGYKLIF
                     GIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIAAAKTTK
                     TKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYA
                     SGADIVYQVAGGTGAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASN
                     FVLASSIKEVGKSVELIATKTSKGKFPGGNVTTYGLKDGGVDIATTNLSDDAVKAIKE
                     AKAKIISGDIKVPSK"
     gene            1760..3295
                     /locus_tag="VBD27_001681"
     CDS             1760..3295
                     /locus_tag="VBD27_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025675.1"
                     /translation="MTQNVIEMKEITKKFGDFVANDHINLTVEKGEIHALLGENGAGK
                     STLMNMLAGLLEPTDGQIFINGQPVTIDSPSKSSQLGIGMVHQHFMLVEAFTVAENIV
                     LGNETTQNGVLDIKTAAKEIKELSEKYGLSVNPNAKISDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPSEIKELMTIMKSLVKEGKSIILITHKLDEIRAVADKVTVIRRGK
                     SIETVPVAGASSQQLAEMMVGRSVSFRTEKKEANPTDIILSVKDLVVEENRGGVLAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVTSGQIVIKGKDVTKFSSRQITEL
                     SVGHVPEDRHRDGLVLDMTMAENLALQTYYKEPLSHKGILNFAKIKEYARQLMTEFDV
                     RGAGEHVLARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DKGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVKPDQTNKQELGILMAGGKIEKEE
                     RDV"
     gene            3288..4349
                     /locus_tag="VBD27_001682"
     CDS             3288..4349
                     /locus_tag="VBD27_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025676.1"
                     /translation="MSKKAQKIAVPLISVVLGIILGAIIMLIFGYDPLWGYEGLFQTA
                     FGSIKNIGEIFRAMGPLILIALGFSVASRAGFFNIGLPGQALSGWIAAGWFALSHPDM
                     PRPAMILCTIIIGIVAGGITGAIPGILRAYLGTSEVIVTIMMNYIVLYSGNAIVQRVF
                     PKSIMRTSDSSVYVSANASYQTDWLSSLTNNSRINIGIFIAIIAVVLVWFLLNKTTLG
                     FEIRSVGLNPNASEYAGMSAKRTIILSMIISGAFAGLGGVVEGLGTFENVFVQPSSLA
                     IGFDGMAVSLLAANSPIGILFAAFLFGVLSVGAPGMNIAGIPPELIKVVTASIIFFVG
                     VHYIIEYVIKPKKQMKGGK"
     gene            4351..5307
                     /locus_tag="VBD27_001683"
     CDS             4351..5307
                     /locus_tag="VBD27_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025677.1"
                     /translation="MSLTTIFALLFSSMLIYATPLIFTSIGGTFSERGGIVNVGLEGI
                     MVIGAFSGVVFNLEFASVFGDATPWISVLVGGLVGLIFSVIHAVATVNFRADHIISGT
                     VLNLMAPSLAVFLIKVLYNKGQTDNIQESFGKFNFPILSDIPFVGDIFFKGTSLVGYI
                     AILFSFLAWFILYKTRFGLRLRSVGEHPQAADTLGINVYLMRYSGVLISGFLGGIGGA
                     VYAQSISVNFAATTILGPGFISLAAMIFGKWNPIGAMLASLFFGLSQSLAVIGSHLPL
                     LSNIPTVYLQIAPYVLTIIVLAAFFGQAVAPKADGINYIKTK"
BASE COUNT         1676 a          806 c         1160 g         1771 t
ORIGIN      
        1 aagacttggt tactcaataa taaatacttc tgtgtttgat aagaagataa taaacgtgtt
       61 ctaaaaattt tgccagtaaa cattatgaag cgaaaggtgc agctactaaa aatgggaaat
      121 atagagttga aaaaattggc tgtaaaagcg agtgaaaatg catacgttcc ttactcgaaa
      181 tttccagtag gcgcagcatt gcgtacagca gaaggaaaaa tatttacagg atgtaacgtt
      241 gaaaatatca gttatggatt agcaaactgt gctgagcgta ctgcaatttt taaagctgtt
      301 tcagaaggtt ataaagattt ttcggaaatt gctatttatg gaaatactga agaaccaatt
      361 tctccttgcg gtgcatgccg tcaagtgatg gtggaatttt ttaataagaa tgctaaagtt
      421 acattgattg ctaaaaatgg taagacggtc gagacgacgg tcggtgagtt gttaccatac
      481 tcgtttgttg atttaaaata atttaaatca gttgctcttt taagcattta gcttaaatat
      541 taagtttttt aggagggttc atttcatgaa caaaaaaatt gccgggatcg gcttggcttc
      601 gattgcagta cttagtttag ctgcatgtgg acatcgtggt gcttctaaat ctggtggtaa
      661 atcagatagc ttgaaggttg caatggtaac agataccggt ggtgttgatg ataaatcatt
      721 taaccaatct ggttgggaag gtatgcaagc ttggggcaag aagaatggcc ttaaaaaagg
      781 agctggtttt gactatttcc aatcggcaag tgaatctgat tatgcaacta acttagatac
      841 agctgtgtct agtggttata aattgatttt cggtattgga ttttctcttc atgatgctat
      901 tgataaagca gcagacaata acaaagatgt taattacgtc atcgttgatg atgttattaa
      961 agggaaagat aatgttgcaa gtgttgtctt tgcggataat gaatcagctt acttagcagg
     1021 tattgcagcc gctaaaacta ccaaaacaaa aacagttggc tttgtaggtg gtatggaatc
     1081 tgaggttatt acccgttttg aaaaaggttt tgaagcaggt gtcaaatcag ttgataaatc
     1141 aattaaaatt aaagttgact atgctggttc attcggtgat gctgctaagg gtaagacaat
     1201 tgcagccgca caatatgctt ctggcgcaga tattgtttat caagtagctg gtggaactgg
     1261 tgcaggtgta ttcagtgaag caaaatcacg caatgaatct ttaaaagaag ctgacaaagt
     1321 ttgggtatta ggtgtagacc gtgaccaagc agcagaaggt aagtatactt ctaaagatgg
     1381 taaggcttct aactttgttt tggcttcatc aattaaagaa gttggaaaat cagttgaatt
     1441 gattgctact aaaacaagta aaggtaaatt cccaggtggt aatgtaacaa cttatggttt
     1501 gaaggatggt ggagttgata tagcaactac aaacctttca gatgacgccg ttaaagcgat
     1561 taaagaggct aaagctaaaa ttatttcagg agatatcaaa gttcctagca aataatatct
     1621 ttaagaactt tcaaaaaagc gattagaaat cgcttttttg aagctgagat aaacttatat
     1681 ctcagtaaaa tccatcagtc gttaacgatt ggtggatttt actgaactat aaatgaattt
     1741 ttctgaaagg gagaaccaca tgacacagaa tgtcattgaa atgaaagaaa tcactaagaa
     1801 attcggtgat tttgtggcaa atgatcatat caatttgacg gttgaaaaag gtgagataca
     1861 tgctctgctt ggcgaaaacg gtgcagggaa atcaacttta atgaacatgt tagcaggttt
     1921 actagaacca acagacggtc aaatttttat taatggtcag cctgtaacta ttgattcacc
     1981 ttccaaatcg tcacaattgg ggattggtat ggtacatcag catttcatgc ttgttgaagc
     2041 atttactgta gctgaaaata ttgttctagg aaatgaaacc acccaaaatg gtgttttaga
     2101 cattaagaca gcagctaaag aaataaagga actatcagag aaatatggtt tgtcagtaaa
     2161 tccaaatgct aaaatatctg atatttctgt aggagcacag cagcgtgttg aaattttgaa
     2221 gacactttat cgcggtgcag atattttaat ttttgatgag ccgacggcgg tgcttacacc
     2281 atcagaaata aaagaattga tgacaattat gaagagcctt gttaaagaag gcaaatccat
     2341 tatactaatt acccataaat tggatgaaat tcgggcagtt gctgataaag ttactgtgat
     2401 tcgccgtggt aaaagtattg aaacagtccc tgttgcaggc gcaagttctc aacaactggc
     2461 ggaaatgatg gttggacgat ctgtttcctt cagaactgaa aaaaaggaag ctaatccgac
     2521 tgacataatc ctttctgtaa aggatttagt tgtagaagaa aatagaggcg gcgttctagc
     2581 tgttaaaaat ctgtcgttag atgttcgagc aggtgaaatt gtaggtatag ctggtattga
     2641 tggtaatgga caaagtgaat taattcaagc tattacaggt ttaagaaagg taacttctgg
     2701 tcaaattgta attaaaggta aagatgtcac aaaattttca agtcgacaaa ttacagaatt
     2761 aagtgtaggt catgttccag aagatcgtca tagagatggt ttggttctag atatgacgat
     2821 ggcagaaaat ttagctttac aaacttatta taaagagcca ttgagtcata aaggtatcct
     2881 taattttgct aaaattaaag agtatgctcg tcaattaatg acagaatttg atgtgcgtgg
     2941 tgctggtgaa catgttttag caagaggatt ttctggtggt aaccaacaaa aagctattat
     3001 tgctcgtgaa gtggatcgcg atccagacct actcattgtt agccaaccga ctcgtggtct
     3061 agatgttggt gctatcgagt atattcataa acgtttgatt gaagagcgcg ataaagggaa
     3121 agcggtctta gtagttagct ttgagttaga tgaaatctta aacttatcag atcgtattgc
     3181 agttattcat gatggtaaaa ttcaaggaat tgttaaaccc gaccaaacca acaagcaaga
     3241 attgggtatt ttgatggctg gtggaaaaat tgaaaaggag gagagagatg tctaagaagg
     3301 cgcaaaaaat tgcagttcct ctgatttcag ttgtactagg aatcattcta ggagctatta
     3361 ttatgctgat atttggttat gatccacttt ggggttatga gggattgttc caaacagcat
     3421 tcggtagtat aaaaaatatt ggtgaaattt tccgtgcaat gggaccatta attttaattg
     3481 ctctaggttt ctcagttgcc agtcgtgcag gtttctttaa tattggactt ccagggcaag
     3541 ccttatcagg atggattgcc gcaggttggt tcgctctttc acacccagat atgccacgtc
     3601 cagcaatgat tctttgtact attattattg gtatcgttgc tgggggaatc actggagcta
     3661 ttccaggtat tctcagagca taccttggaa caagtgaagt tattgtaact attatgatga
     3721 actatattgt tctttattca ggaaatgcta ttgtacaacg tgttttccca aaaagcatta
     3781 tgcgtacaag tgactcgagt gtatatgttt ctgccaatgc atcataccag acagattggt
     3841 tatctagttt aacgaataat tcacgtatca atattggtat cttcattgct attattgcag
     3901 ttgtgttagt ttggttccta ttaaataaaa caacgcttgg atttgaaatt cgttcagttg
     3961 gtttaaatcc aaatgctagt gagtacgctg gtatgtctgc aaagcgtaca attattctat
     4021 caatgattat ttctggagct tttgcaggac ttggtggtgt tgttgaagga ctaggtacat
     4081 ttgagaatgt atttgtacag ccatcctcgt tagctattgg ctttgacggt atggcagtaa
     4141 gtctactcgc agcgaatagt ccaataggta ttttatttgc agcatttcta tttggtgttc
     4201 taagtgttgg tgcgcctggt atgaacattg caggtattcc acctgagtta attaaagttg
     4261 taacagcttc aatcatcttc ttcgtaggtg tccattacat tattgagtat gttattaaac
     4321 ctaaaaaaca aatgaaaggt ggtaagtaaa ttgagtttaa caacgatttt tgcattgcta
     4381 ttttcatcaa tgctaattta cgcaacacct cttatcttta caagtattgg gggaaccttc
     4441 tctgaacgtg gtggtatcgt caacgttggt ttagaaggaa ttatggtaat tggagctttc
     4501 tcaggcgttg tatttaattt agagtttgct tctgtttttg gtgatgcgac tccatggatt
     4561 tctgtactag ttggtggttt agttggttta attttttctg ttatccatgc agttgcgaca
     4621 gttaattttc gtgcagatca tattatcagc ggaaccgttc ttaatttaat ggcaccttca
     4681 ttagctgttt tcttgattaa ggttctttat aataaaggtc aaactgataa tattcaagaa
     4741 tcatttggaa aatttaattt ccctatacta tcagatattc cttttgttgg tgatatcttc
     4801 tttaaaggaa caagcttagt cggttatatt gctattcttt tctcattcct tgcttggttt
     4861 attctatata aaactcgctt tggattaaga ttacgctcag ttggagaaca tcctcaagca
     4921 gcagacacct taggtattaa tgtttatctt atgcgttact ctggtgtctt gatttcaggt
     4981 ttccttggtg gaataggagg agctgtatat gctcaatcta tttctgttaa ctttgcagcg
     5041 acaactatat taggaccggg ctttatctcc ttagcggcga tgatttttgg taagtggaat
     5101 ccaattggtg caatgcttgc tagtctattc tttggtttat cgcaaagttt agcagttatc
     5161 ggtagccatt taccactttt gagcaatatt ccaacagttt atctacaaat tgctccatat
     5221 gtcctcacta tcattgtatt agcagctttc tttggacaag cagtagctcc taaggcggat
     5281 ggtattaatt atattaaaac gaaataaata aaaagaaact agtataagct agtttctttt
     5341 tattgtttgt gaatttgtga aaaaaatact ttgtgaaaat ttcacataaa ttataataca
     5401 ataacaataa aat
//
LOCUS       DATRQA010000125         5324 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.125, whole genome shotgun sequence.
ACCESSION   DATRQA010000125 DATRQA010000000
VERSION     DATRQA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.125"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            99..1331
                     /gene="arcA"
                     /locus_tag="VBD27_001684"
     CDS             99..1331
                     /gene="arcA"
                     /locus_tag="VBD27_001684"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEO7025678.1"
                     /translation="MTQTHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRNEGVEVLYLENLAAESLTNQEIREQFIDEYIGEANVRGRA
                     TKKAIRELLLNIKDNKELIEKTMAGIQKSELPEIPSSEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGNGVSLNHMFSETRNRETLYGKYIFTHHPEYGGKVPMVYDREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVYSVTYENQDLHIEEEKGDLADLLAKNLGVEKVELI
                     RCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKINGSELV
                     RGRGGPRCMSMPFEREDL"
     gene            1337..1767
                     /locus_tag="VBD27_001685"
                     /pseudo
     CDS             1337..1767
                     /locus_tag="VBD27_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1783..2796
                     /gene="argF"
                     /locus_tag="VBD27_001686"
     CDS             1783..2796
                     /gene="argF"
                     /locus_tag="VBD27_001686"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO7025679.1"
                     /translation="MTQVFQGRSFLAEKDFSREEFEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTIKENFGKLEGITLVY
                     CGDGRNNVANSLLVAGTLMGVNVHIFSPKELFPAEEIVKLAEGYAKESGAHVLVTDNV
                     DEAVKGADVFYTDVWVSMGEEDKFKERVELLQPYQVNMELIKKANNDNLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            2859..4286
                     /gene="arcD"
                     /locus_tag="VBD27_001687"
     CDS             2859..4286
                     /gene="arcD"
                     /locus_tag="VBD27_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEO7025680.1"
                     /translation="MEKEKKLGLLPLTMLVIGSLIGGGIFDLMQNMSSRAGLVPMLIA
                     WVITAIGMGTFVLSFQNLSEKRPDLTAGIFSYAKEGFGNFMGFNSAWGYWLSAWLGNV
                     AYAALLFSSLGYFFKFFGNGNNIISIIGASIVIWVVHFLILRGVNTAAFINTVVTFAK
                     LVPVIIFLISALLAFKFNIFSLDIWGNGLHQSIFNQVNSTMKTAVWVFIGIEGAVVFS
                     GRAKKHSDIGKASILALFTMISLYVLISVLSLGIMSRPELANLKTPAMAYVLEKAVGH
                     WGAILVNLGVIISVFGAILAWTLFAAELPYQAAKEGAFPKFFAKENKNKAPINSLLVT
                     NLCVQAFLITFLFTQSAYRFGFALASSAILIPYAFTALYQLQFTLREDKSTPGHQKNL
                     IIGILATIYAIYLIYAGGFDYLLLTMIAYTLGMILYIKMRKDDKLPIFVRYEKISAIV
                     ILALCLLCIIEIMTGQIDIAAILAG"
     gene            4307..5263
                     /gene="arcC"
                     /locus_tag="VBD27_001688"
     CDS             4307..5263
                     /gene="arcC"
                     /locus_tag="VBD27_001688"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO7025681.1"
                     /translation="MVKEHQKIVVALGGNAILSTDASAKAQQEALINTSKSLVKLIKE
                     GHDVIVTHGNGPQVGNLLLQQADSDSEKNPAMPLDTCVAMTEGSIGFWLQNALNNELQ
                     EQGIDKEVATVVTQVIVDEKDQAFTNPTKPIGPFLSEEDAKKQAQETGSKFKEDAGRG
                     WRKVVPSPKPVGIKEASVIRRLVDSGVVVISAGGGGVPVIEDANTKALKGVEAVIDKD
                     FASQTLSELVDADLFIVLTGVDNVFVNFNKPNQEKLEEVTVSQMKQYITENQFAPGSM
                     LPKVEAAIAFVENKPEARAIITSLENIDNVLAENAGTQIVVN"
BASE COUNT         1732 a          923 c         1014 g         1655 t
ORIGIN      
        1 cttccctatt ttatattcaa aagaaagtag aatgtaagtg tagatataga gataaatcct
       61 gtttcaatgc aaatctctaa ctaagaaagg agtaaaatat gactcaaaca catccaattc
      121 atgttttctc agaaattgga aaacttaaaa aagttatgct acaccgtcca ggtaaagaaa
      181 tagaaaatct gatgcctgat tatttagaga gactcttgtt tgatgatatc ccatttttgg
      241 aagatgcaca aaaagaacac gatgcgtttg cacaagcact tcgcaacgaa ggagtcgaag
      301 tactttacct tgaaaatctt gctgccgagt cgctaacaaa ccaagaaatt cgtgaacagt
      361 ttatcgatga gtacattggc gaagcaaatg ttcgcggtcg tgctactaaa aaagctattc
      421 gagaattatt actcaatatc aaagataata aagaacttat cgaaaaaact atggcaggta
      481 tccaaaagtc tgagctacct gaaatcccat cttctgaaaa aggtctaact gatcttgtcg
      541 aatctaatta tccatttgcc atagatccga tgccaaatct ttactttaca cgtgatccat
      601 ttgcaactat tgggaatgga gtttcattaa accatatgtt ctctgaaaca cgtaatcgcg
      661 aaactttata cggtaaatat atctttacac atcacccaga gtacggtgga aaagtcccta
      721 tggtctatga tcgtgaagaa acaactcgta ttgaaggtgg agatgagctc gtcctttcta
      781 aagatgtcct tgccgtgggt atctctcaac gtactgatgc tgcctctatt gaaaaactct
      841 tagtcaatat ttttaaacaa aatttaggtt ttaaaaaggt tttagcattt gaatttgcaa
      901 ataatcgtaa atttatgcac cttgacacag tctttacaat ggttgactat gacaagttta
      961 caattcaccc agaaattgaa ggcgatcttc gcgtttactc tgtaacttac gaaaaccaag
     1021 atcttcatat tgaagaagaa aaaggtgatt tggcagatct tctagcgaaa aacctaggag
     1081 ttgagaaagt ggaattgatt cgttgcgggg gtgacaactt agtcgctgct ggacgtgaac
     1141 aatggaatga tggttcaaat acattaacta tagcaccagg tgtcgttgtc gtttacaaca
     1201 gaaatacaat cacaaatgca atcttagagt ccaaaggatt gaaattaatt aagattaatg
     1261 gaagtgaatt agttcgtggg cgtggtggac cacgctgcat gtcaatgcca tttgaacgtg
     1321 aagaccttta aaatttatga agctattagt caatcacttg ccttaccaaa gagctgcaag
     1381 cctatatatt cgcttttctg ttttcgttat cgaaagaaat ataaaaatga aagaagaatt
     1441 cgatgataat gacgagcaag atactattta tgctgttctt tatgatggaa acagccagtt
     1501 tcaacaggac gctttcttcc tgaaactcaa acagaagctc gtttgactcg aattgcgact
     1561 ttaaaaggtt atcgtggtaa cggctatggt accaaaatta ttatagcact agaaaattat
     1621 gcaaaagaaa atggttatca ctatctaact atacatgctg aattgacagc aaaagatttc
     1681 taccagacac ttgggtatca agctactggg aatatttata tggaagacgg ggaagcctgt
     1741 caaacactcg aaaaatattt aatttaaagg agacattaac atatgacaca agtatttcaa
     1801 ggacgtagtt tcttagcaga aaaagatttt tctcgtgagg aatttgaata tcttattgat
     1861 ttttcagctc atttaaaaga ccttaaaaaa cgtggtgttc ctcatcatta tcttgaaggt
     1921 aaaaatattg ctctcttatt tgaaaaaaca tctactcgta ctcgcgcagc ctttacaact
     1981 gcagcaattg acctaggcgc tcatccggaa taccttggtg caaatgatat tcaacttggt
     2041 aaaaaagaat caacagaaga tactgctaag gttttaggac gtatgtttga tggtattgaa
     2101 ttccgtggtt ttagccaaag aatggttgaa gagcttgctg aattttctgg agtacctgtc
     2161 tggaatggtt taacagatga atggcatcca acacaaatgc tagctgacta ccttactatc
     2221 aaagaaaact tcggtaaact tgaaggtatt actcttgttt actgtggtga cggacgtaac
     2281 aatgttgcca actcgctttt agtggctggg actttgatgg gggtcaatgt acacatcttt
     2341 tctccaaaag aacttttccc tgctgaagag attgttaaat tggctgaagg atatgccaaa
     2401 gaatctgggg ctcacgttct cgttactgat aatgtagacg aagctgtaaa gggagcagac
     2461 gtcttttaca ctgatgtctg ggtatcgatg ggagaagaag ataagttcaa agaacgcgtt
     2521 gaacttcttc aaccatatca agtaaacatg gaactgatta aaaaagctaa taatgataat
     2581 cttatcttct tacactgctt acctgcattc catgatacaa ataccgttta tggcaaagac
     2641 gtcgctgaaa aatttggggt caaggaaatg gaagttactg atgaagtctt ccgtagcaaa
     2701 tatgctcgtc atttcgacca agctgaaaat cgtatgcaca ctattaaagc tgtaatggct
     2761 gcaacccttg gaaatctttt cattccaaaa gtttaaatta aacgactata gggagttgac
     2821 atatagtcaa ttccctattt taggaaatga ggtataaaat ggaaaaagaa aaaaaattag
     2881 gtcttttacc actaacaatg cttgtcattg gctctcttat cggtggcgga atctttgatt
     2941 taatgcaaaa tatgagttcc agagccggtt tggtaccaat gcttattgct tgggtaatta
     3001 ctgctatcgg gatgggaact ttcgttttaa gttttcaaaa tttatctgaa aaaaggccgg
     3061 atctaacagc tggaatcttt agttacgcta aagaggggtt tggaaacttt atgggattta
     3121 actctgcatg gggttattgg ttatcagctt ggcttggaaa tgttgcctac gctgcactct
     3181 tattcagttc actcggttat ttctttaaat tctttggtaa tggaaataat atcatctcaa
     3241 ttattggagc aagtatagtt atttgggttg tccatttctt aattttaaga ggtgttaata
     3301 cagctgcatt tattaatacc gtagttacct ttgcaaaatt agtacctgtt attattttct
     3361 taatttcagc gttattagct ttcaaattta acatttttag tcttgatatc tggggaaatg
     3421 gattacatca atcaattttc aaccaagtca attcaactat gaaaaccgct gtttgggtat
     3481 ttattggtat tgagggcgcc gttgtcttct caggtcgtgc taaaaaacac tctgatattg
     3541 gtaaagcaag tatcctagca ttattcacta tgatttcact ttatgtattg atttctgttt
     3601 tatcacttgg tatcatgtca cgtccagaac ttgcaaactt aaaaacacca gctatggctt
     3661 acgttctaga aaaagctgtt ggtcactggg gtgctatctt agttaacctt ggtgttatca
     3721 tttcagtatt tggcgctatt cttgcttgga ctttatttgc agcagaatta ccatatcaag
     3781 ctgctaaaga aggtgctttt cctaaatttt ttgcaaaaga aaataaaaac aaagctccaa
     3841 tcaactcact cttagtcact aatctttgtg tacaagcatt cttaatcacg ttcttattca
     3901 cacaaagtgc ttatcgtttt ggtttcgcat tagcatcatc tgctatctta attccttatg
     3961 cttttacagc actatatcaa ttacaattca cactccgtga ggataagtca actccaggac
     4021 atcaaaagaa tttaattatc ggtatcctcg ctacaatcta tgctatttac cttatctacg
     4081 ctggtggttt tgattactta cttttgacaa tgattgctta tactctaggt atgattctct
     4141 atattaaaat gagaaaagat gacaagcttc ctatctttgt gagatatgaa aaaattagtg
     4201 ccattgtcat tctagcgctt tgcttactat gtattattga aattatgact ggacaaattg
     4261 acattgctgc aatcctagca ggttagtcat tagaaagagg gataaattgg taaaagaaca
     4321 tcaaaaaatc gtagttgcct taggtggtaa cgctattcta tcaacagatg cttcagcaaa
     4381 agcacaacaa gaagcattaa tcaacacatc aaaatcactg gtcaaactga ttaaagaagg
     4441 ccatgatgtc attgttactc atggaaatgg tccccaagtt ggtaacttat tgctacagca
     4501 agcagattca gattctgaaa agaatccagc aatgcctttg gatacttgtg ttgccatgac
     4561 agaaggaagt attggttttt ggttacaaaa tgctcttaac aatgaattac aagaacaagg
     4621 cattgacaaa gaggttgcaa cagtggtaac tcaagttatt gtagatgaaa aagatcaagc
     4681 tttcactaat ccaacaaaac ctattggccc attcttatct gaagaagatg ccaagaaaca
     4741 agcccaagaa acaggttcaa aatttaaaga agatgctggt cgtggatggc gtaaagtcgt
     4801 tccttcacca aaaccggttg gtatcaaaga agcttctgtt attagacggt tagtggactc
     4861 tggtgtggtt gttattagcg caggtggcgg aggtgtacca gttattgaag atgctaatac
     4921 aaaagcatta aaaggggttg aagctgttat tgacaaagac tttgccagtc aaactttatc
     4981 agaacttgtt gatgccgact tattcatcgt cttaactggc gttgacaacg tttttgttaa
     5041 ttttaataaa cctaatcaag aaaaattaga ggaagttact gtttcacaaa tgaaacaata
     5101 tatcactgaa aatcaatttg cccctggcag tatgctacca aaggtagaag ctgcgattgc
     5161 ttttgttgaa aataaaccag aagcgcgtgc tatcattact tcacttgaaa atattgataa
     5221 tgtcttagca gaaaatgcag ggacacaaat tgttgtaaat taaacaatgt agattaccat
     5281 tacatacact ccttattaat aacaaagtaa tgtcactcct tagg
//
LOCUS       DATRQA010000126         5310 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.126, whole genome shotgun sequence.
ACCESSION   DATRQA010000126 DATRQA010000000
VERSION     DATRQA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.126"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            30..1277
                     /locus_tag="VBD27_001689"
     CDS             30..1277
                     /locus_tag="VBD27_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO7025682.1"
                     /translation="MEKNTWKKLLVSTAALSVVAGGAIAATHSNSVDAASKTTIKLWV
                     PTDSKASYKAIVKKFEKENKGVTVKMIESNDSKAQENVKKDPSKAADVFSLPHDQLGQ
                     LVESGVIQEIPEQYSKEIAKNDTKQSLTGAQYKGKTYAFPFGIESQVLYYNKTKLTAD
                     DVKSYETITSKGKFGQQLKAANSYVTGPLFLSVGDTLFGKSGEDAKGTNWGNEAGVSV
                     LKWIADQKKNDGFVNLTAENTMSKFGDGSVHAFESGPWDYDAAKKAVGEDKIGVAVYP
                     TMKIGDKEVQQKAFLGVKLYAVNQAPAGSNTKRISASYKLAAYLTNAESQKIQFEKRH
                     IVPANSSIQSSDSVQKDELAKAVIEMGSSDKYTTVMPKLSQMSTFWTESAAILSDTYS
                     GKIKSSDYLKRLKQFDKDIAKTK"
     gene            1375..2745
                     /locus_tag="VBD27_001690"
     CDS             1375..2745
                     /locus_tag="VBD27_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7025683.1"
                     /translation="MNKKLISLDGMSLKEVFQKGDLATKLSFLIMGLANLKNKQIVKG
                     LLFLISEILFLITFVYQVIPAVKGLISLGTQEQGMTTKTVDGIKIQVATQGDNSMLML
                     IFGLASLIFCCVFAYIYWSNIKSAAHLLTLKEEGREIPSFKKDIKSLTDGRFHMTLMS
                     IPLIGVLLFTILPLVYMICLAFTNYDHNHLPPKSLFDWVGFANFGNIFSGRMASTFFP
                     VLSWTLIWAVFATVTNFFFGIILALLINTKGLKFKKMWRTIFVITMAVPQFISLLIMR
                     NLLSDAGPVNALLIKWGLISSAHPLPFLSDPVWAKFSIIFVNMWVGIPVTMLVATGII
                     MNLPAEQIEAAEIDGANKFQVFQSITFPQILLIMTPTLIQQFIGNINNFNVIYLLTQG
                     GPTNSTYYQAGSTDLLVTWLYNLTVTAADYNLASVVGILIFILSAVFSLLAYTRTNSY
                     KEGAAK"
     gene            2745..3581
                     /locus_tag="VBD27_001691"
     CDS             2745..3581
                     /locus_tag="VBD27_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7025684.1"
                     /translation="MNKKKRLNLTFVYILLIVLSIMWLFPIVWVVLTSFRGEGSAFVN
                     YFIPKTWTLDNYAKLFTQNTFPFGQWFLNTLFVATCTCILSTLITVAMAYSLSRIKFK
                     HRNGFLKLALVLNMFPGFMSMIAVYYILKALNLDQTLTALIFVYSAGAALTFYIAKGF
                     FDTIPYSLDESAMIDGATRLDIFLKITLPLSKPIIVYTALIAFMGPWMDFIFAKVILG
                     DATSKYTVAIGLFSMLQQDTINQWFMSFTAGSVIIAIPITILFMFMQKYYVEGITGGS
                     VK"
     gene            3812..5284
                     /locus_tag="VBD27_001692"
     CDS             3812..5284
                     /locus_tag="VBD27_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7025685.1"
                     /translation="MEKTKSFFGHPRGLSTLFFTEMWERFSYYGMRAILLYYMYYSVS
                     QGGLGMDKTVAASIMAVYGSLVYLSSVIGGFVSDRILGSRKTVLYGGILIMLGHIALA
                     TPFGQTALFISIALIILGTGLLKPNVSEMVGNLYGENDSRRDAGFSIFVFGINLGAFI
                     SPIVVGYLGQEVNFHLGFSLAAIGMFFGLLQYTLDGKKYLTEESLRPNDPLSPEEKSS
                     LYKKVGLILIGIVIVLILLHLMHMLTIEVIIDIFSIIAIAIPIIYFIKILSSKKISSV
                     ERSRVWAYIPLFIASILFWSIEEQGSVVLALFADEQTKLYLNFFGHHINFPSSYFQSM
                     NPLFIMLYVPFFAWLWAKWGSKQPSSPKKFAYGLFFAGASFLWMMLPGLLFGVNAKVS
                     PLWLTMSWAIVIVGEMLISPVGLSATSKLAPKAFQAQMMSIWFLSNAAAQAINAQIVK
                     LYTPDTQILYYGVVGGITVVFGFILLFYVPRIEKLMSGVK"
BASE COUNT         1620 a          982 c          921 g         1787 t
ORIGIN      
        1 ttaaaatatt ctctaggagg agaaccaaaa tggaaaaaaa tacttggaaa aaattacttg
       61 ttagtactgc tgctctttca gtagttgcag gaggagcaat tgctgctact cactctaact
      121 cagttgatgc tgcttcaaaa acaactatca aactttgggt cccaacagat tcaaaagcgt
      181 cttataaagc aattgttaaa aaattcgaga aggaaaacaa aggcgttact gtaaaaatga
      241 ttgagtctaa tgactccaaa gctcaagaaa acgtaaaaaa agacccaagc aaggcagccg
      301 atgtattctc acttccacat gaccaacttg gtcaattagt agaatctggt gttatccaag
      361 aaattccaga gcaatactca aaagaaattg ctaaaaacga cactaaacaa tcacttactg
      421 gtgcacaata taaagggaaa acttatgcat tcccatttgg tattgaatct caagttcttt
      481 attataataa aacaaagtta actgctgacg acgttaaatc atacgaaaca attacaagca
      541 aagggaaatt cggtcaacag cttaaagcag ctaactcata tgtaacaggt cctcttttcc
      601 tttctgtagg cgacacttta tttggtaaat ctggtgaaga tgctaaaggc actaactggg
      661 gtaatgaagc aggtgtttct gtccttaaat ggattgcaga tcaaaagaaa aatgatggtt
      721 ttgtcaactt gacagctgaa aatacaatgt ctaaatttgg cgatggttct gttcatgctt
      781 ttgaaagtgg accatgggat tacgacgctg ctaaaaaagc tgtcggtgaa gataaaatcg
      841 gtgttgctgt ttacccaaca atgaaaatcg gtgacaaaga agttcaacaa aaagcattct
      901 tgggcgttaa actttatgcc gttaaccaag cacctgctgg ttcaaacact aaacgaatct
      961 cagctagcta caaactcgct gcatatctaa ctaatgctga aagtcaaaaa attcaattcg
     1021 aaaaacgtca tatcgttcct gctaactcat caattcaatc ttctgatagc gtccaaaaag
     1081 atgaacttgc aaaagcagtt atcgaaatgg gtagctcaga taaatataca acggttatgc
     1141 ctaagttgag tcaaatgtca acattctgga cagaaagtgc tgctattctt agcgatactt
     1201 acagtggtaa aatcaaatct agcgattacc ttaaacgtct aaaacaattc gataaagaca
     1261 tcgctaaaac aaaatagtac gtctagagta atgagatgag gtgagtactc ttcctttcaa
     1321 ttgagagtgc ttcccatctc ttattttgta aaataacaat taaaggagac cattatgaat
     1381 aaaaaactga tctcacttga tggtatgtcc ctaaaagagg tctttcaaaa aggtgattta
     1441 gcaactaaac tatcattctt aattatggga cttgctaacc tcaaaaataa acaaattgtc
     1501 aagggacttt tatttttaat cagtgaaatt ctgtttttaa ttacctttgt ttaccaagtt
     1561 atcccagctg taaaaggtct tatttcctta gggacccaag aacaaggtat gacaactaaa
     1621 actgtggatg gtattaaaat ccaagttgcg acacaaggtg ataactctat gttaatgcta
     1681 atttttggat tagcttcgct tatcttctgt tgcgtatttg cctatattta ctggtcaaac
     1741 attaaaagcg cagcacattt attgaccttg aaagaagaag gaagagaaat tccatccttc
     1801 aaaaaagata ttaaaagctt gacagatgga cgcttccata tgactttaat gtctattcct
     1861 ttaatagggg tgcttctatt tacaatcctt ccgttagttt atatgatttg tttggcattt
     1921 actaactatg atcataacca cttaccgcca aaaagtttat ttgactgggt tggttttgct
     1981 aacttcggta atatctttag cgggcgtatg gccagtactt tcttccctgt tctatcttgg
     2041 acattaattt gggctgtttt cgctactgta acgaatttct tctttggtat tattcttgcc
     2101 ttactaatta atacaaaagg tttgaaattc aaaaagatgt ggcgtactat ttttgttatt
     2161 accatggctg tccctcaatt tatctctctc ttaatcatga gaaacctctt aagtgacgct
     2221 ggaccggtta acgccttgtt aataaaatgg ggacttattt cttcagcaca tccccttcca
     2281 ttcctttctg atccggtctg ggcgaaattc tcaatcatct ttgttaatat gtgggttggt
     2341 attcctgtaa caatgcttgt tgctactggt atcatcatga atttacctgc agaacaaatt
     2401 gaagctgctg aaattgatgg ggcgaataag ttccaagtct ttcaatcaat tactttccca
     2461 caaattttgt tgattatgac tcctacttta attcaacaat ttatcggaaa tattaacaac
     2521 ttcaacgtta tctacctact cactcaaggt ggaccaacaa actcaacata ctaccaagca
     2581 ggtagcaccg atttacttgt cacttggcta tacaatttga cagtaactgc tgcagattat
     2641 aacttagcat ctgttgttgg tatcttaatc tttattcttt cagcagtatt tagtttacta
     2701 gcttacacaa gaacaaattc ttacaaggaa ggggcagcta aataatgaat aagaaaaaac
     2761 gtcttaacct tacttttgtt tatattttat tgatagtact atcaattatg tggctctttc
     2821 ccattgtttg ggtcgttctc acaagcttcc gtggtgaagg aagtgccttt gtcaactact
     2881 ttattcctaa aacttggaca ttagacaact acgctaaact cttcacccaa aacacattcc
     2941 catttggtca atggttctta aatacattat ttgtcgctac atgtacttgt atactatcaa
     3001 ctttaatcac tgtagcaatg gcttattctc tcagcagaat caaattcaaa caccgtaatg
     3061 gtttcctaaa gctagcttta gtcttgaata tgtttcctgg gttcatgtca atgattgcag
     3121 tttactacat cctaaaagct ctaaatctag accaaacatt aaccgcttta atatttgtct
     3181 attctgcagg agcagcatta accttctaca tcgcaaaagg tttctttgat actattccat
     3241 actctttgga tgagtctgct atgattgatg gggcgacccg tttggatatt ttccttaaaa
     3301 tcaccctacc attgtcaaaa cccattatcg tttatactgc attaatcgct tttatgggcc
     3361 cttggatgga ctttatcttt gcaaaagtta ttttaggtga tgctacgagc aagtacacag
     3421 tcgctattgg tctcttttca atgttgcaac aagataccat caaccagtgg ttcatgtcat
     3481 ttacagcagg atctgttatt attgcaattc caatcacaat acttttcatg ttcatgcaaa
     3541 aatactatgt tgaaggtatt acaggtggat ctgtaaaata atcttcagat aatttaaaga
     3601 cacgacattt ttatttcact gtggtttctt ttctaaaaaa ggcttgggtt ctattgaatt
     3661 caagcctttt tctaagctag atagttaaaa aacttggttt accaagggtg ttaacctcta
     3721 caaaaaaata acttgatagt gctaagtaat ttgacagttg cttctattta ctatattatt
     3781 aatagtgtta tctacaaaag gagcgttttt catggaaaaa acaaaatcat tctttggaca
     3841 tcctcgtggt ttgtccactc ttttctttac tgaaatgtgg gaaagattct catactatgg
     3901 tatgcgtgct attttgctgt actatatgta ctatagtgtt tctcaaggtg gccttggtat
     3961 ggacaagact gtcgctgcat caatcatggc tgtctatggt tcactggttt atctctcatc
     4021 agtaattggt ggttttgtca gcgaccgtat tctaggtagt cgtaaaactg ttctgtatgg
     4081 tggtattcta atcatgctag gtcatattgc tctagctaca ccatttggtc aaacagctct
     4141 attcatttcg attgctctaa ttatccttgg tactggacta ttaaaaccaa atgtatcaga
     4201 gatggttggt aatttatatg gagaaaatga ttctcgtcgt gatgctggtt ttagtatctt
     4261 tgtttttggt attaaccttg gtgcttttat ttcacccatt gtagtggggt acctcggaca
     4321 agaagtaaat ttccatcttg gtttctcact tgctgctatt ggtatgttct ttggtctcct
     4381 ccaatatacc ttagatggaa aaaaatattt gactgaagag agtctcagac caaacgatcc
     4441 tttaagtcct gaagaaaagt cctctctata taaaaaagtt gggcttatcc ttattggtat
     4501 tgttattgta cttattctac ttcacttgat gcatatgcta acaattgaag taattatcga
     4561 tatttttagt attattgcaa tcgccatccc aattatttat tttatcaaga ttttaagtag
     4621 taaaaagatt tcttctgttg agcgttctcg agtgtgggca tatatccctc tctttatcgc
     4681 ctcaattcta ttttggtcaa ttgaagaaca aggttcagtt gtcttagcct tatttgcaga
     4741 tgaacaaaca aaactttacc ttaacttctt tgggcatcat attaatttcc catcaagtta
     4801 tttccaaagt atgaaccctc tcttcattat gctttatgta ccattctttg cttggttatg
     4861 ggctaaatgg ggaagtaagc aaccttcatc acctaaaaaa tttgcgtatg gacttttctt
     4921 tgctggagct tcattcttat ggatgatgct accaggttta ctctttggag ttaacgctaa
     4981 agtaagccct ctttggttaa caatgagttg ggctattgtc atcgttgggg aaatgctaat
     5041 ctcaccagtt ggattatcag caactagtaa gctcgcacct aaagcattcc aagctcaaat
     5101 gatgagcatc tggttcttaa gtaatgctgc agcacaagct attaacgctc aaatcgttaa
     5161 attgtacaca cctgatactc aaattcttta ttatggtgtt gttggtggta taacagttgt
     5221 atttggattt atcctcttat tttatgttcc acgcattgaa aaactaatgt ctggagttaa
     5281 ataaagacat ctaaaaagaa ctaggttaca
//
LOCUS       DATRQA010000127         5291 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.127, whole genome shotgun sequence.
ACCESSION   DATRQA010000127 DATRQA010000000
VERSION     DATRQA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.127"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1245)
                     /locus_tag="VBD27_001693"
     CDS             complement(<1..1245)
                     /locus_tag="VBD27_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025686.1"
                     /translation="MNNKGVGGDGVQIYQYYIKMDNNKPYLSPKDKTTVEKLEDRWKK
                     ITFKVQDTGIGLKDVYLQSVKYVGGGNNNLDLITPPGFKKEDKKVEKPKLDRPPGIDL
                     PAPTSMRSFDYSTPPGTKPSKPKDSLSTPPGFPDLNTPPDEAPKDSKKDAIEDKSGAI
                     KYAKSLQLSFVDDPILASKVNGKILQVESDGKLVIPRNALSANQFDDTSLKIYRNNNR
                     NKEITITTDYFADTKYVNITAVDYLSNTTFEQLATGETVDYHAIVFSSFAAIKDKGGK
                     IYVNDKLQETSRIALKDKSVKIGIELPNDVRHIDSLSVRRLNEVKTVDNILKNDEQDI
                     NLSKTYQLKYNPTNRRLEFTINNINSSSEIMTTFKDGKMPELVEQKDVSLDINDMDMS
                     KFKTIRLGRKDSEFKGQLIAKTG"
     gene            complement(1291..3948)
                     /locus_tag="VBD27_001694"
     CDS             complement(1291..3948)
                     /locus_tag="VBD27_001694"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017709.1,HMM:NF018035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO7025687.1"
                     /translation="MTKPQGAVAEKATPAVPKPQKVRVIVEFNKESILDYATEQKKTV
                     AQLNQADVEKKLQSIKQEQDKVLKNIEKSVHFDSSKVKRYDAIINGVALDIQAQEIEK
                     LKTIADVRRVYVSQEYVQTKPLLSSSGQLIGLPEVWNNSQYKGEGTVVAVIDSGVDFK
                     HQALKIKEPNRAKYNKTSIEKLIHEKNLKGKFYSEKVPYGYNYYDYNDNLKDSYGVMH
                     GMHVTGIVGANDDNQKLYGVAPNAQILAMKVFSDDQQNPTTFTDVWLKALDDAILLKA
                     DVVNMSLGTPAGFVHEGKDYPELEVIARACKAGIVIAVAAGNEGNITDGNTYGVKPLA
                     ENYDTALIANPALDDNTLAVASMENLKKHAHVLKFKDKKSGTEVTEVINLHVAPNASK
                     TIIGLAVDLGAEAPSELSKHFDLSGKIAMLEIPEDNKSNGFLEKVQAITKLNPAAILL
                     YNNAKVKDDLGSQLLVESEAAKFNIARITRSTYNNIKNNSNKIITILTERQAIDNSLA
                     GQLSSYSSWGPTPDLRLKPEITAPGGHIFSTVEDNQYADKSGTSMAAPQVAGAAAVLK
                     QYITDKKIPVDNAADFIKLLLMNTAQPIINKQSKDGKTPYFVRQQGSGAMNLAKALVT
                     TVVATVTGTNDNNADGKLELRELKEKKFKARILLRNFGKTNKTYIISSEAIADPVDEK
                     GFRTQNSEHLVSKKADAVTRKVTVEAGKTLAVDLDVDYSDAEALTRNNFLEGYLNLKD
                     TEGVADLHLPFLGFYGSWTEQKAIDAFEGISEIGNGDKKRRVQFYVNKETNKTSSTFT
                     TNGMLSLPIYNNTVFFSPNSPFYDKAGVRIAALRNMEYVQYSIIDPDTNKEVRVLGRS
                     HDVRKLYRLDYRNSFAMMPDSIWDGKIKD"
     gene            complement(4000..4515)
                     /locus_tag="VBD27_001695"
     CDS             complement(4000..4515)
                     /locus_tag="VBD27_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025688.1"
                     /translation="MTFYKKTDKFGFRKSKVCRSLCGALLGTVAVVSLATASTEIHAD
                     EATTSPTTVTKVPQPVQADTTALNTSKTHSTQATTTPVEAKENKVVKSETVQSESRVM
                     PRDKVVERPETVKASVNSDVSQPITTTPPTINEKTVEIPNLAQDTKKVAPKVTVTPEV
                     VNQPKQLPKYE"
     gene            complement(4549..4890)
                     /locus_tag="VBD27_001696"
     CDS             complement(4549..4890)
                     /locus_tag="VBD27_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025689.1"
                     /translation="MSEFLSAKPIGHNDSSNTILRIIENTKYIHHYDGKVVVEIPPQK
                     EPIKVFFMDSRLHYLEFSDICRYVTVVLKNSTVDNITNLGLDNHIVYDNGDDMVDPLP
                     SIQHGLFLNDD"
BASE COUNT         1527 a          986 c          863 g         1915 t
ORIGIN      
        1 tccagttttt gcaataagtt gtcccttaaa ttcagaatcc tttcgtccaa gtcgaatagt
       61 tttaaactta ctcatgtcca tatcgtttat atccaaagaa acatcttttt gttcaaccaa
      121 ttctggcatc tttccatctt tgaaagtggt catgatttct gaacttgagt taatgttatt
      181 aatagtaaac tctagacgac gatttgtcgg gttgtatttt aattggtaag ttttgctgag
      241 attaatgtct tgttcatcat ttttcaagat attatcaaca gttttaacct cattcaaacg
      301 acgaacagat aaactatcaa tatgtctgac atcatttggt aattcaatac caatcttaac
      361 agatttatct ttaagcgcta tacgagaagt ttcttgcaat ttatcgttaa cataaatctt
      421 accacccttg tctttaatag cagcaaagct tgaaaataca atggcatggt aatctactgt
      481 ttcaccagta gctaattgct caaaagtagt attgctcaaa tagtcaaccg ctgtgatatt
      541 gacatatttt gtatctgcaa aataatctgt tgtgatagta atttctttat tgcgattatt
      601 attacgataa attttaagac tagtgtcatc aaattgatta gctgacaaag catttctagg
      661 aatgactaat ttgccatcag attcgacttg taatattttg ccatttactt tgctagctaa
      721 aatagggtca tcaacaaagc taagttgaag agacttagca tatttaattg ctcctgattt
      781 atcttcaata gcgtcttttt tactatcctt tggtgcttca tccggcggcg tgtttaaatc
      841 tgggaaacct ggaggagttg ataaactatc tttgggtttg cttggcttag ttcccggtgg
      901 ggttgaataa tcaaaacttc tcattgaagt tggtgctggt aaatcaattc ctggtggacg
      961 gtctaatttt ggtttttcaa cttttttatc ttctttttta aatcctggag gtgtgataag
     1021 gtctaaatta ttattgccac caccaacata cttaacagat tgaagataaa cgtctttcaa
     1081 accaatgcca gtatcctgaa ctttgaaagt aatttttttc cagcgatctt ctaacttctc
     1141 tacagtagtc ttatctttgg gacttaagta aggtttattg ttgtccattt tgatatagta
     1201 ttgataaatt tggacaccat cgccaccgac acctttatta ttcaactgta ctttaatttg
     1261 ataaatgtat tgtttatccc cttttgcaat ttaatcttta atcttaccat cccaaataga
     1321 atctggcatc atggcaaaac tattacgata atctaggcga taaagttttc taacgtcgtg
     1381 tgatctacca agtacacgaa cttctttatt ggtatctgga tctataatag aatattgaac
     1441 atactccata ttacgcaaag ctgcaatacg aactcctgct ttgtcataga aaggactatt
     1501 aggagagaaa aatactgtat tgttatagat tggaagactc aacatgccgt tagttgtaaa
     1561 ggttgatgag gtcttgttgg tttctttatt aacatagaat tgaacgcgac gttttttgtc
     1621 accgttacca atttcagaaa tgccttcaaa agcatcaata gctttttgct ctgtccatga
     1681 accgtaaaaa cctaaaaatg gtaagtgtaa atcggctaca ccttcagtat cttttaagtt
     1741 aagataacct tccaagaaat tattgcgagt taaagcctct gcatcagaat aatccacatc
     1801 aaggtcaaca gctaatgtct tacctgcttc tacagtaact ttccttgtaa ctgcatcagc
     1861 tttttttgat actaggtgtt ctgaattttg cgtacgaaat cctttttcat caactgggtc
     1921 cgcaatcgct tctgatgaga tgatatacgt tttattagtt tttccaaagt tacgaagtag
     1981 aatgcgtgct ttaaattttt tctcttttaa ttcacgtaac tccaacttac catctgcatt
     2041 attatcgtta gtccctgtta cagttgcaac gactgtcgta acaagagctt tggcaagatt
     2101 cattgctccg ctaccttgtt ggcgcacaaa gtaaggtgtt ttgccatctt ttgattgttt
     2161 attgataatt ggctgtgcag tgttcatcaa gagtaattta ataaaatcag cagcattatc
     2221 aacaggaatt tttttatctg taatgtattg ttttaaaact gcagctgccc cagctacctg
     2281 aggtgcagcc attgatgttc ctgatttatc agcatattga ttatcctcca cagttgagaa
     2341 aatatgacca cctggagctg taatctcagg ttttagacgt aagtctggtg taggtcccca
     2401 agaagaataa gaagataatt gtcctgctaa actattatca atagcttgac gctctgttaa
     2461 aatagtaata attttattgc tattattttt gatattgtta tacgtgcttc tggtaatgcg
     2521 agcaatatta aatttagctg cttctgattc aactaataat tgactgccta aatcatcttt
     2581 aaccttcgca ttattgtaaa gcaagatagc agctggattt aacttagtaa tcgcctgtac
     2641 tttttctaaa aatccattag atttattatc ttctggaatt tcaagcatgg cgattttacc
     2701 acttaaatca aaatgttttg acaattccga aggagcttct gctcccaaat ctacagccaa
     2761 tccaataata gtttttgaag cgtttggggc aacatgaaga ttaatgactt ctgtaacttc
     2821 tgttccagat tttttatctt taaatttcaa aacatgagca tgctttttaa ggttctccat
     2881 agaggcaaca gccaaggtat tatcatcgag ggctgggttg gcaatcaagg ctgtatcata
     2941 attttctgct agcggcttaa caccataagt attaccgtca gtgatatttc cttcattacc
     3001 tgctgctact gcaatgacga ttcctgcctt acacgctctt gcaataactt caagttcagg
     3061 atagtctttc ccctcatgta caaatccagc aggtgttcca agactcatat taacaacatc
     3121 agcttttagt aaaatagcat cgtctagagc ttttagccac acatctgtga aggttgtggg
     3181 attttgttgg tcatctgaaa ataccttcat cgctaaaatt tgagcatttg gtgcaacacc
     3241 atacaatttt tgattatcat cattagctcc tacgatacct gttacatgca taccatgcat
     3301 tacaccgtag ctatccttga ggttatcatt atagtcataa tagttataac cataaggtac
     3361 tttttcggaa tagaattttc ctttgagatt cttttcatga atcagttttt caatactagt
     3421 tttattatac ttagcacgat ttggttcttt gatttttagt gcttggtgtt tgaaatcaac
     3481 ccctgaatca ataactgcaa caacagttcc ctcaccttta tactgggagt tgttccaaac
     3541 ttctggcaaa ccaataagtt gccctgagga agatagtaaa ggttttgtct gaacatattc
     3601 ttgtgagaca tagacccttc taacatcagc aatcgttttt aatttttcaa tttcttgagc
     3661 ttgaatatca agtgcgacac cattaataat agcatcgtaa cgcttcactt tgcttgaatc
     3721 aaagtgaaca cttttttcaa tattcttaag aactttatct tgctcttgct tgatgctttg
     3781 taattttttc tcaacatcag cttgatttaa ctgtgcaact gtttttttct gttctgtcgc
     3841 ataatctaag atagattcct tattaaattc aacaattact cttacttttt gaggctttgg
     3901 cacagccgga gtagctttct cagcaaccgc cccctgtggt ttagtcacag taactgcctt
     3961 agttgcttct gtactcttat tgcctttctc tgagttttct tactcgtatt taggcaactg
     4021 ctttggctga ttaacaactt ctggtgtaac agttactttt ggagctacct tcttagtatc
     4081 ttgagctaag ttaggaatct caactgtttt ttcattaatt gttgggggtg tggttgtaat
     4141 aggttggcta acgtctgagt taacactagc ttttactgtt tctggccttt ctactacctt
     4201 atctctaggc attactcggc tctcagattg aacagtttca cttttgacaa ccttattctc
     4261 cttagcttca acaggagttg tagttgcttg tgtactgtga gtcttagagg tatttagagc
     4321 tgttgtgtct gcttgaactg gctgaggtac tttcgttaca gtggtaggtg aggttgttgc
     4381 ttcatcagcg tgaatctctg tacttgctgt agctagacta acaactgcta ctgtacccaa
     4441 taaagcaccg cataggctgc ggcacacttt acttttccta aatccaaatt tgtccgtctt
     4501 tttataaaat gtcatggctt cctcgtttct aaatttttgt ctacatcttt aatcatcatt
     4561 aagaaataat ccatgctgaa tactcggcaa cggatcaacc atatcatcac cattgtcata
     4621 aacaatatga ttatctagcc ctaaattggt tatattatca acggtactat ttttcaaaac
     4681 aacagttaca taccgacata tgtctgaaaa ttctaaataa tgaagacgtg aatccataaa
     4741 aaagactttg ataggctctt tttgaggagg aatttcgaca acaacttttc catcatagtg
     4801 atgaatatat ttcgtattct ctataattct taaaatagta ttagatgaat cattatgacc
     4861 aataggtttg gctgaaagga attcactcat aaaagaacct cacattactt aaccggtttg
     4921 ttaaccagct aacgaaattt tagcactcct ctaagtactt gtcaatggca aatgttaagt
     4981 taaaatcgtt ttcagcaata agttctttta tcttttttta cattagaaaa tatttcagtt
     5041 tttattattt ctaattatta tcacaaaaaa acacctctat tttctgattt tctcataaaa
     5101 tgtggaaatg atcagtttat catatcaact ctaaccataa ttgttaaaga ctacccaagg
     5161 tattataaaa agcccttgaa atacttcaaa gtgcttttta tagatgcata tgtaggaaca
     5221 acatgttgcg agttagttat ctggtaataa ttcttttaag taaaccgctt ctactttaaa
     5281 agtattatca a
//
LOCUS       DATRQA010000128         5238 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.128, whole genome shotgun sequence.
ACCESSION   DATRQA010000128 DATRQA010000000
VERSION     DATRQA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.128"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(46..1365)
                     /locus_tag="VBD27_001697"
     CDS             complement(46..1365)
                     /locus_tag="VBD27_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEO7025690.1"
                     /translation="MKKYDVIVLGFGKAGKTLAAKLATQGKSVAMVEEDDKMYGGTCI
                     NIGCIPTKTLLVSASKNHDFQEAMTTRNEVTSRLRAKNFAMLDNKDTVDVYNAKARFI
                     SNKVVELTGGADKQELTADVIIINTGAKSVQLPIPGLADSQHVYDSTAIQELAHLPKR
                     LGIIGGGNIGLEFATLYSELGSKVTVIDSQSRIFAREEEELSEMAQDYLEEMGISFKL
                     SADIKSVQNEDENVVISFEDEKLSFDAVLYATGRKPNTEGLALENTDIKLTERGAIAV
                     DEYCQTSVENIFAVGDVNGGPQFTYISLDDSRIVLNYLNGDKDYSLKNRGAVPTSTFT
                     NPPLATVGLDEKTAKEKGYQVKSNSLLVSAMPRAHVNNDLRGIFKVVVDTETNLILGA
                     RLFGAESHELINIITMAMDNKIPYTYFQKQIFTHPTMVENFNDLFNF"
     gene            complement(1519..2016)
                     /locus_tag="VBD27_001698"
     CDS             complement(1519..2016)
                     /locus_tag="VBD27_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO7025691.1"
                     /translation="MTTYFDNFLKTNQAYADLHGTAHLPIKPKTKVAIVTCMDSRLHV
                     AQALGLALGDAHILRNAGGRVTADVLRSLVISQQQLGTREIVVLHHTDCGAQTFTNEA
                     FAAQLQRDLGVDMHGHDFLPFNDIEESVREDVAKLHASPLIPNDVVISGAIYDVDTGR
                     MVEVK"
     gene            complement(2152..3516)
                     /gene="radA"
                     /locus_tag="VBD27_001699"
     CDS             complement(2152..3516)
                     /gene="radA"
                     /locus_tag="VBD27_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO7025692.1"
                     /translation="MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKN
                     ARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
                     KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAKRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGE
                     IGLTGEIRRVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKA
                     VFST"
     gene            complement(3678..4124)
                     /locus_tag="VBD27_001700"
     CDS             complement(3678..4124)
                     /locus_tag="VBD27_001700"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO7025693.1"
                     /translation="MSKVRGFELVSQFSNKELLPKRETAHAAGYDLKVAKKTVIEPGE
                     ITLVPTGIKAHMQPGEVLYLYDRSSNPRKKGIVLINSVGVIDGDYYNNQVNEGHIFAQ
                     MQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK"
     gene            complement(4187..4272)
                     /locus_tag="VBD27_001701"
     tRNA            complement(4187..4272)
                     /locus_tag="VBD27_001701"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4236..4238),aa:Leu,seq:aag)
     gene            complement(4319..5212)
                     /locus_tag="VBD27_001702"
     CDS             complement(4319..5212)
                     /locus_tag="VBD27_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7025694.1"
                     /translation="MIVVILFLISVAASFYFFHVAQVRDDKSFISNGQRKPGNSLYAY
                     DKSFDKLLKQKIEMTNQNIKQVAWYVPAAKKTHKTVVVVHGFANSKENMKAYGWLFHK
                     LGYNVLMPDNIAHGESHGQLIGYGWNDRENIIKWTEMIVDKNPSSQITLFGVSMGGAT
                     VMMASGEKLPSQVVNIIEDCGYSSVWDELKFQAKEMYGLPAFPLLYEVSTISKIRAGF
                     SYGQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKELYIVKGAKHAKS
                     FETEPEKYEKRISSFLKKYEK"
BASE COUNT         1644 a         1104 c          798 g         1692 t
ORIGIN      
        1 gataaaaaaa gtaaagcaaa attgccttac tttttaaaaa tattcttaaa agttaaacaa
       61 atcattaaaa ttctctacca ttgttgggtg agtgaaaatt tgtttttgga aataagtata
      121 tgggatttta ttatccattg ccattgtgat gatattaatt aattcatggg attctgcacc
      181 aaataagcga gctcccaaaa taagatttgt ctcagtatct accacaactt taaaaatacc
      241 tctaaggtca ttattaacat gtgcacgtgg catggcagaa acaagaaggc tattagattt
      301 aacttgatat cctttttcct tagcggtctt ttcatctaga cctaccgttg ctaaaggagg
      361 atttgtaaag gttgaagtcg gtactgctcc tcgatttttt agagaataat ccttatcacc
      421 attcaaataa tttaagacaa tacgtgagtc atctaaagaa atatatgtga attgagggcc
      481 gccattaaca tcaccaactg caaaaatatt ctcaacagaa gtttggcaat attcatcaac
      541 agcaatagct ccacgttctg tcaatttaat atctgtattt tcaagtgcta aaccttctgt
      601 atttggctta cgtcctgttg catataatac tgcatcaaag cttaattttt catcctcaaa
      661 gctaataaca acgttttcat cttcattttg cacagatttg atatcggctg ataatttaaa
      721 agatatcccc atctcttcta aataatcttg agccatctct gataattcct cttcctcacg
      781 cgcaaagata cgagattgac tatcaataac tgtcactttg ctccctaatt cggaatataa
      841 agtcgcaaat tcaagcccaa tattaccgcc tccaatgata cctaaccgtt taggaagatg
      901 agccaattct tggattgccg ttgaatcata aacatgttga gaatcagcta atcctggaat
      961 tggtaattgt actgacttag caccagtatt gataataata acatcagctg taagttcttg
     1021 tttgtcagct cctcctgtta attcgaccac tttatttgat ataaagcgag ctttagcatt
     1081 ataaacatcc acagtgtctt tattatccaa catagcaaaa tttttagcac gtaaacgact
     1141 tgtaacttca tttctagtag tcatagcttc ttgaaaatcg tgatttttgc tagctgatac
     1201 taaaagcgtt tttgtcggaa tacatccaat gttgatacat gttcccccat acattttatc
     1261 gtcttcttcg accattgcaa cgcttttacc ttgtgttgct aattttgctg ctaatgtttt
     1321 acctgcttta ccaaaaccaa gaacaatgac atcatatttt ttcattttaa atttcctccg
     1381 cttttataat atagtttcag tatagcaaaa gatgtttcct ctgtctgaat tccagcataa
     1441 ggcatgataa gcctaactgt tacctttaaa tctagttttc taaaataaaa aaacccatcg
     1501 ctaaggacag gattttttct atttaacttc caccatacga ccagtatcta catcataaat
     1561 agcaccagaa ataacaacat catttgggat taatggagaa gcatgtaatt ttgccacatc
     1621 ttcacgaacg ctttcttcaa tatcattaaa aggaagaaaa tcatgtccat gcatatcaac
     1681 acccaaatct ctctgtaatt gagcagcaaa agcttcgtta gtaaacgtct gtgctccaca
     1741 atctgtatgg tgtagaacaa cgatttctcg tgttcctaat tgttgttgcg aaataactaa
     1801 agaacgtaga acatcggctg taactcgtcc accagcatta cgtaaaatat gagcatctcc
     1861 taaagctagc cctagagctt gagcaacgtg taaacgagaa tccatacagg ttacaatagc
     1921 aacctttgtc tttggcttga taggtagatg agctgttcca tgtaagtcag cataggcttg
     1981 attggttttt aaaaagttat caaaataagt ggtcatagag atctcctttg ttagtgtaac
     2041 atcattcgat atttactaaa taccaaccct tttatattat cattttgccc attatttttc
     2101 acgaaatgtc tctcgccacc aaaaaagatt cggctgaacc gaatctaatc attatgttga
     2161 aaacactgct tttaagacct gactaacagt tgtcacacca ataacatcaa tgcctttagg
     2221 tatttcaatc ccagctagag aattctttgg tgcgtaaatt ttagtaaagc ccaatttact
     2281 agcctcatta atacgttgct caatacgtgt cacgcgacga atctcaccag tgagtccaat
     2341 ttctccaata aatgattctt gtggatttgt gggtttttct ttgtaactgg aagcaatagc
     2401 tacagcaaca gctaaatcaa ttgctggttc atccaattta acaccacccg ctgattttaa
     2461 ataggcatct tgattttgaa gcaataaacc acaacgtttt tctagaactg ccataataag
     2521 actgacacga ttaaagtcaa gacctgtggt tgtgcgctta gcatttccaa aaacagttgg
     2581 agtcactaaa gcttgaacct ctgctaaaat aggccttgtt ccttccatag taacaacaat
     2641 agcagatcct gttgcaccgt ctaaacgttc ttcaagaaaa acttgacttg gattcaaaac
     2701 ttcaactaga ccacccgact gcatttcaaa gatacctatt tcattggtcg aaccaaaacg
     2761 gtttttgact gcccgtaaaa tacggaacgt gtgatgacgc tccccttcaa aataaagaac
     2821 cgtatccacc atatgttcta gcattcgagg gcctgctaat gttccctcct tagtcacatg
     2881 acctacaata aaagtagcaa tattattagt tttagctaac tgcatcaact cagcggtcac
     2941 ttcgcgaact tggctaacag agccttggac acttgatact tcgggactca taatagtttg
     3001 tatagaatca ataataagaa aatctggttt aatcttctca atctccgacc gaatactttg
     3061 catattagtc tcggcataaa ggtaaaattc attatcgata tcgcctagac gctcacttcg
     3121 taacttaatc tgttctgcag attcctcacc tgagacatat aatactgtac ctttattagc
     3181 tagctgagtt gatacttgta gtaataaagt agatttacct atcccagggt ctcctccaat
     3241 cagaaccaaa cttcctggta caacaccgcc tcctagaaca cggttaaatt catccatatc
     3301 agtcttagtt ctagaatagt taattgagga aacatctttt aacttcgtcg gtcgggactt
     3361 ttcaccgttc aaactaactc gtgcattttt aacttcttga acttcaacct cttctacaaa
     3421 tgaagaccag gctgagcaat ttggacaacg tcccaaatac ttaggtgatt ggtaaccaca
     3481 ctcttgacag gtgaagactg attttttctt agccactcag gcctccttta taacttctaa
     3541 attaatatga tagcttggta ccaaataact agtacactca ttatatcaca ccaagttaga
     3601 gaaaatataa gtttactctc atcaaaaaag gctaagaagt aagtctagcc ttttctaaca
     3661 ttattcgtaa aataatttta ttttccagta ctaccaaaac cacctgtacg cattcctgta
     3721 gcctgatcgt catctgccaa taagaatggg gcaaaaacag cctgaacaat ccgttcccct
     3781 tcttctaaaa taacagcctg atcagtaata ttttgcatct gtgcaaaaat atgtccttca
     3841 ttgacttggt tattatagta gtcaccatca ataacaccaa ctgaatttat taggacaatc
     3901 ccttttttac gtggattcga tgaacgatca taaaggtaca ggacctcccc tggttgcatg
     3961 tgtgctttaa taccggtcgg gaccaatgta atttcaccag gttcaataac cgttttttta
     4021 gctactttta aatcatagcc agctgcgtga gctgtttctc gttttggtaa taactcttta
     4081 ttgctaaatt ggctaactaa ttcaaagcca cgtacttttg acatgtgata ctccatcttt
     4141 atcttaaata tgcaaaaagt cgagttccaa atgaactcga ctagtctgcg gagagaggga
     4201 cttgaaccct cacgaccaaa agcggtcaca agatccttag tcttgcgcgt ctgccaattc
     4261 cgccatcccc gcttaacaac ataataaatt atatcaactt atcatattgt tgtcaatatt
     4321 atttttcata ttttttcaaa aaactagaga tacgtttctc atatttttct ggctctgttt
     4381 caaaagattt cgcatgtttt gcccctttta caatataaag ctctttctta cctgctgtag
     4441 ctttatagtt gtcataaacc atacttgttg gaacaaaatt atccttatca ccatgaataa
     4501 agagggctgg taaattattc tttttcaatt gttcgacact acttgcttgt ccatacgaaa
     4561 aacctgctct gattttagaa attgttgaaa cttcatataa gagtgggaag gctggtaaac
     4621 catacatctc tttagcctga aattttaatt catcccaaac actagaataa ccacaatctt
     4681 caatgatatt aacaacctga ctaggtaatt tttcaccact agccatcatg actgttgctc
     4741 cacccattga aacaccaaat aaagtaattt ggcttgatgg attcttatcc actatcattt
     4801 ctgtccattt gataatgttc tcgcggtcgt tccagccata gcctatcaac tgcccatgac
     4861 tttcaccatg tgcaatgttg tcaggcataa gaacattgta tcctaactta tgaaacagcc
     4921 aaccatatgc cttcatattc tctttgctat tcgcaaaacc atgaacgaca acaactgtct
     4981 tatgagtttt cttagcagca ggaacatacc aagcaacttg ctttatattt tggtttgtca
     5041 tttctatttt ttgctttaat agcttatcaa aggatttatc ataagcatat aaagagtttc
     5101 caggcttacg ttgaccattt gaaataaagg atttatcatc tcgaacttgg gcaacgtgga
     5161 aaaaataaaa actagctgct acactaatta aaaacaaaat aacaacaatc attttaataa
     5221 actttgataa tcgaatct
//
LOCUS       DATRQA010000129         5172 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.129, whole genome shotgun sequence.
ACCESSION   DATRQA010000129 DATRQA010000000
VERSION     DATRQA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.129"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(195..268)
                     /locus_tag="VBD27_001703"
     tRNA            complement(195..268)
                     /locus_tag="VBD27_001703"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(232..234),aa:Asn,seq:gtt)
     gene            complement(298..369)
                     /locus_tag="VBD27_001704"
     tRNA            complement(298..369)
                     /locus_tag="VBD27_001704"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:Glu,seq:ttc)
     gene            561..634
                     /locus_tag="VBD27_001705"
     tRNA            561..634
                     /locus_tag="VBD27_001705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:595..597,aa:Arg,seq:tct)
     gene            complement(707..1186)
                     /gene="rlmH"
                     /locus_tag="VBD27_001706"
     CDS             complement(707..1186)
                     /gene="rlmH"
                     /locus_tag="VBD27_001706"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO7025695.1"
                     /translation="MKLKIITVGKLKEKYLKEGVAEYQKRLNRFSKIETIELADEKTP
                     DKASISENQRILDIEGERILSKIGERDYVIGLAIEGKQLPSESFSHLIDQKMISGYST
                     ITFVIGGSLGLSQKVKKRADYLMSFGLLTLPHQLMKLVLMEQIYRAFMIRQGTPYHK"
     gene            1387..2616
                     /locus_tag="VBD27_001707"
     CDS             1387..2616
                     /locus_tag="VBD27_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO7025696.1"
                     /translation="MKKKLVSSLLKCSLIIIVSFAGGAFASFVMNHNDNIPNGGVTKT
                     SKVNYNNITPTTKAVKKVQNSVVSVINYKQQESRSDLSDFYSHFFGNQGGNTDKGLQV
                     YGEGSGVIYKKDGKNAYVVTNNHVIDGAKQIEIQLADGSKAVGKLVGSDTYSDLAVVK
                     IPSDKVSNIAEFADSSKLNIGETAIAIGSPLGTEYANSVTQGIVSSLKRTVTMTNEEG
                     QTVSTNAIQTDAAINPGNSGGALINIEGQVIGINSSKISSTSNQTSGQSSGNSVEGMG
                     FAIPSNDVVKIINQLESNGQVERPALGISMAGLSNLPSDVISKLKIPSNVTNGIVVAS
                     IQSGMPAQGKLKKYDVITKVDDKEVASPSDLQSLLYGHQVGDSITVTFYRGENKQTVT
                     IKLTKTSKDLAKQRANN"
     gene            2714..3487
                     /locus_tag="VBD27_001708"
     CDS             2714..3487
                     /locus_tag="VBD27_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO7025697.1"
                     /translation="MEYLETININHIAPNPYQPRLEFNTKELEELADSIKINGLIQPI
                     IVRPSAVFGYELVAGERRLRAAKLAKLESIPAIIKSYNNDDSMQLAIVENLQRSNLSP
                     IEEAKAYSQLLQKKSMTHEELAKYMGKSRPYISNTIRLLNLPPLITSAIEEGKLSSGH
                     ARALLSLPDASQQKDWYQRILTEDISVRRLEKLLKQEKKTNHKSLQNKDVFLKHQENE
                     LAQFLGSKVKLTINKDGAGNIKIAFNNQEELNRIINTLK"
     gene            3696..5057
                     /gene="dnaA"
                     /locus_tag="VBD27_001709"
     CDS             3696..5057
                     /gene="dnaA"
                     /locus_tag="VBD27_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO7025698.1"
                     /translation="MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVI
                     TLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEV
                     NTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGK
                     THLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDD
                     IQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPV
                     NITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFK
                     HAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVL
                     ARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKN
                     KIR"
BASE COUNT         1822 a          894 c          874 g         1582 t
ORIGIN      
        1 aggaaaaggc aaagtaacat taatttttat cccttacggc tttaaatttg gggtaggcct
       61 atctattact ggaatagtat tatttactgt ctattattta aaatttggaa aaaataaaat
      121 aggatgagag tactctcatc ctattttatt aacaaaaaaa cgccgacaaa tcaacgttta
      181 gatgtgtctt atcactccgg cagcaggact cgaacctgcg acatcatgat taacagtcat
      241 gcgctactac caactgagct atgccggata aataaaaaac tgcaaagcag ttatcaatag
      301 tccgtacggg attcgaaccc gtgttaccgc cgtgaaaagg cggtgtctta accccttgac
      361 caacggacca taggttaggt gtttccttag tactcttaat attatatcag aaaaaaaagg
      421 tttgtctata cttttgttca ataattttta caaaaaaatt gaacctactt atgagcaaaa
      481 aaatatattt ccaactcata aatagtacat atgaaaactt gacagcctgc ctaatatttt
      541 ggtagaataa cagagttgat ggttccatag ctcagctgga tagagcattc gccttctaag
      601 cgaacggtcg caggttcgaa tcctgctgga atcagataat gaaagcctag atagattatc
      661 taggcttttt tatttgtttt tagcggttgt ttcataaatg gaacgattat ttgtggtaag
      721 gagtgccttg acgaatcata aaagcacgat aaatttgttc catcaagact aatttcataa
      781 gttgatgagg taaggttaac agtccaaaac tcattaaata atcagcacgt tttttcactt
      841 tttgagataa acccaaacta ccaccaataa caaaagtaat agtagagtag ccggatatca
      901 ttttttgatc gattagatgg ctaaatgatt cagatggcaa ttgtttccct tctatagcca
      961 aaccaatgac ataatccctc tcacctattt tactcaaaat acgttcccct tctatatcta
     1021 aaattctttg attttcagat atactagctt tatcaggtgt cttttcatca gctagctcaa
     1081 ttgtctcaat tttagaaaag cgattcagac gtttctgata ctctgcaaca ccttctttaa
     1141 ggtatttctc tttcaatttt ccaactgtaa ttatttttaa tttcatagct acattataac
     1201 ataatccaca atattttcac aatttatcca catgtttatc cttacatatc cccttatttt
     1261 ataggcaatc tataactata atcaaggtta tccacaactt gtggatattt ccttttttta
     1321 gcttttaagg tataattaag aagcttgtca taagattagt ataaaaattt acggaggtag
     1381 ctatttgtga aaaaaaaatt agtctcatca cttctaaagt gttctctaat cattattgtt
     1441 agctttgctg gtggagcatt tgctagtttt gtcatgaatc ataatgacaa tattccaaat
     1501 ggtggtgtca ctaaaactag taaagtaaat tataataaca taacgcctac aacaaaagct
     1561 gttaaaaagg tacaaaatag tgttgtttct gttatcaatt ataaacaaca agagagtcgt
     1621 tctgacctat cagacttcta tagtcatttt tttggtaatc aggggggcaa cactgataag
     1681 ggcttacaag tttacggtga aggctctgga gtcatctata aaaaagatgg taaaaatgcc
     1741 tatgttgtca ctaataacca cgtcattgat ggggctaaac aaattgaaat tcaactagct
     1801 gatggctcaa aagcagttgg gaaacttgtt gggtcagata cctactctga tttagccgtc
     1861 gtcaaaattc catcagataa agtttcaaat attgcagaat ttgctgattc atcaaaactc
     1921 aacattggtg aaactgctat agcgatcgga agccctcttg gaactgagta tgcaaattct
     1981 gtaactcaag gtattgtatc tagtttaaaa agaactgtaa caatgactaa tgaagaagga
     2041 caaacagttt ctacaaatgc tatccagacg gatgctgcta tcaatcctgg taattcaggt
     2101 ggagcactta tcaatattga aggacaggtt attggaatta attctagtaa aatttcttct
     2161 acatcaaatc aaacctcagg acaatcgtca ggaaatagcg ttgaaggtat gggatttgcc
     2221 attccttcaa atgatgttgt taagattatc aatcaacttg agagtaacgg acaagtagag
     2281 agacctgctc taggtatttc tatggctgga ttaagtaatt taccatccga tgttattagt
     2341 aaactgaaaa tcccaagtaa tgttactaat ggtattgtag tagcatctat ccaatctggc
     2401 atgccagctc aaggcaaact aaagaaatac gatgtcatta ctaaagttga cgataaagaa
     2461 gtagcatctc caagtgattt acaaagttta ctctatggcc accaggtagg ggattccata
     2521 acagtaacct tttatcgtgg tgaaaataaa caaacagtca ctataaaact tactaaaact
     2581 agtaaagatt tagctaaaca acgagcaaat aactaaatca attctagtta ttttaactaa
     2641 gatatagtaa aattaaaggg gttggtcaga tagaccaatc cctttagtaa gtctatagaa
     2701 tctgaggtct aaaatggaat atttagaaac gatcaacatc aatcatattg ctccaaatcc
     2761 gtatcaacct cgcctagaat ttaatacaaa agaactagaa gaattagccg actctattaa
     2821 aattaatggc cttattcaac ccattattgt aagaccgtca gcagtctttg gttacgaact
     2881 ggtagccgga gaacgacggt taagagccgc aaagttagct aaattggaaa gtatacctgc
     2941 aattataaaa tcctataata atgatgatag tatgcaatta gctattgtcg aaaatttaca
     3001 acgatctaat ttgtcaccca ttgaagaggc taaagcctac tctcaacttc tacaaaagaa
     3061 aagtatgaca catgaagagt tagcaaaata tatgggaaaa tctcgtccct atatatcaaa
     3121 tacgatacga ctactaaatt taccgccact aattacttca gctattgaag aaggaaaact
     3181 ttcctctggt catgcgcgtg cactactcag tctaccagat gctagtcaac aaaaagattg
     3241 gtatcagaga atcctcactg aagatattag tgtaagacga ctagagaagt tattaaaaca
     3301 agaaaagaaa acaaatcata agtcacttca aaataaagat gtctttctaa aacatcaaga
     3361 aaatgagtta gctcaatttc taggtagcaa agtgaaactt actattaata aagatggtgc
     3421 tggcaatata aagattgctt tcaataacca agaagaatta aacagaatta tcaacactct
     3481 aaaataaggg tgttgattat ttttttattt aatcaaactt atccacaagg tattttgcta
     3541 tttttctgtt gattctctaa acttttctaa tttccacagt ctgtggaaaa ctttaattaa
     3601 cattgttgat tttattcttc aacatctgtg gaaaacttta tttttttatg gtacaatata
     3661 acaataatta tccacaagac aataaggaag aagctatgac ggaaaacgaa caactatttt
     3721 ggaatagagt actagagcta tctcgttctc aaatagcacc agcagcttat gaattttttg
     3781 ttctagaggc tagactcctc aaaattgaac atcaaactgc agttattact ttagataaca
     3841 ttgaaatgaa aaagctattc tgggaacaaa atttggggcc tgttatccta acagctggtt
     3901 ttgaaatttt caatgctgaa attacagcta actatgtctc aaacgattta catttacaag
     3961 aaactagttt ttctaactac cagcaatcta gcaacgaagt aaatacttta ccaattagaa
     4021 aaatcgactc taaccttaaa gagaaatata cttttgctaa ttttgttcaa ggagatgaaa
     4081 atagatgggc tgtttcagca tcaattgctg tagctgatag tcctggcacg acttataatc
     4141 ctctatttat ctggggggga cctggtctag gaaagacgca tctactaaat gctattggaa
     4201 atcaagtttt aagagataat ccaaacgcga gggttttata catcactgct gagaatttta
     4261 ttaatgaatt tgtcagtcat attcgtttag attcgatgga agaattaaaa gaaaagtttc
     4321 gcaacttgga cttactcctg attgatgata ttcagtcgct tgctaagaaa accttagggg
     4381 ggacccaaga ggagttcttc aatactttca atgctttaca tacaaacgat aaacaaatcg
     4441 tattgaccag tgaccgaaat ccaaatcaat taaatgatct agaagaacgt ctagtcacgc
     4501 gctttagttg gggactccca gtaaatatca caccacctga ttttgaaaca cgagttgcta
     4561 ttttaaccaa taaaattcaa gaatatcctt atgattttcc tcaagatacc attgaatact
     4621 tagcaggaga atttgattcc aacgtacgtg aattagaagg agccttgaaa aatattagtc
     4681 tagttgctga ctttaagcat gctaaaacta ttacagtaga tatagctgca gaagctatca
     4741 gagcacgtaa aaatgacggt cctattgtta ctgttattcc tatagaagaa attcaaatac
     4801 aagttggtaa attctatggc gtaactgtaa aagagataaa agcaactaaa agaacacaag
     4861 atattgtcct tgcaagacag gtagccatgt acttagctcg tgagatgaca gataacagtc
     4921 tccccaaaat aggtaaagaa tttgggggac gagatcactc aactgttctc cacgcttata
     4981 ataaaataaa aaatatggtt gctcaagatg acaacttacg aattgagata gaaactatca
     5041 aaaataaaat caggtaggct ttgtggataa gtatcagaaa aacacgagac ttttccacag
     5101 gttgtgaaca agttattttc cttatgaata ctaaagttat aatacttatt cacaacttta
     5161 acaggaccta ct
//
LOCUS       DATRQA010000130         5071 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.130, whole genome shotgun sequence.
ACCESSION   DATRQA010000130 DATRQA010000000
VERSION     DATRQA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.130"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            101..361
                     /locus_tag="VBD27_001710"
     CDS             101..361
                     /locus_tag="VBD27_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025699.1"
                     /translation="MLNKLKQFLGFDEVETTETISLDNTLIEVRVLQAENRELKEVIR
                     QKNAILRELSEENIELGRDRRRYADTVATQKRLIDAYESMVG"
     gene            589..726
                     /locus_tag="VBD27_001711"
     CDS             589..726
                     /locus_tag="VBD27_001711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025700.1"
                     /translation="MTKQEYEMVWDLASDRERYEMIDRSIKQGKWACVRYARQLRRKY
                     V"
     gene            723..1415
                     /locus_tag="VBD27_001712"
     CDS             723..1415
                     /locus_tag="VBD27_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HEO7025701.1"
                     /translation="MTKAISFPELQQKMQLEKKKSKDVKYAFRNAEDIYTTFKELKSE
                     WSVIVTDELIELAGKVFVRATAVAFCDETNEKYQSTAYSELSPVPVFNTQKGQIKQMQ
                     EPQWTGAVSSYARKYALQGLFAIGEKDIDEYPVEENQQAPQQQGQNTRQPQQDQIQYV
                     SNEQYNQILNAIKHLSQMVNKPFDELIINIQKHYQIQDFHQVPADHFTTVMNYLQASM
                     AKAQGISDFNDL"
     gene            1426..2493
                     /locus_tag="VBD27_001713"
     CDS             1426..2493
                     /locus_tag="VBD27_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEO7025702.1"
                     /translation="MTKDVTNSILTEIEVNYTPAVINIDYKTVEQQLKAVVDRYANYE
                     VTANTYKADYDERTRLNKFKEALETRRKEIKANINNPYKEFEKRYKAILAPLDEVINN
                     ITVGLNAIDEHERLMRIDVIRATFEDKCMVAGLEKDTFEIKYNEYSLKKYFKTGKFEL
                     KKTTIDEMDALVLAEFDALEEYKASKQAIKEQAQEYDLPADSYIRHLEDGKSLVDVLK
                     IMKGDRDALALRKEQQEAQSKADADRKAELERIAQETANTNIKAVDSDTGEIIEDYPP
                     ELESGGRGTNRPLTQNTAPEAPKFETSKPVNYDLRLTFPHGNPQAKYFKELFEKNGVT
                     SEMLFNGKTQKELTGGTQNVF"
     gene            2483..3049
                     /locus_tag="VBD27_001714"
     CDS             2483..3049
                     /locus_tag="VBD27_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO7025703.1"
                     /translation="MSFNITELIENVKGWSVAKELADADPIKQMQKLNEEWGELNAGK
                     AKGDTAKLYDSIGDVFVVLIILSQQMKFEKIERLIDPTVNGFGYYPKNEVTTDLLLLY
                     GMKEIGMIANRMIDLIHNPGIINTRTAIQFHIRNLTSILYKVAINEDTDIKYCLQLAW
                     DEIKNRQGKMIDGVFVKDEDIKEVDDDE"
     gene            3039..3521
                     /gene="ssb"
                     /locus_tag="VBD27_001715"
     CDS             3039..3521
                     /gene="ssb"
                     /locus_tag="VBD27_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7025704.1"
                     /translation="MMNNVVLVGRLTRDVELKYTQSNVAVATFTLAVNRPFKNEAGER
                     EADFINCVIWRQSAENLANWAKKGSLIGITGAIQTRNYENQQGQRVYVTEVIASNFQL
                     LESRSSQQSNQGYQDNHGGYQQQQSYGNQGGNFRNSGGYQSPFGNSNPMDISDDDLPF
                     "
     gene            3637..4113
                     /locus_tag="VBD27_001716"
     CDS             3637..4113
                     /locus_tag="VBD27_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEO7025705.1"
                     /translation="MTFKTEFEIPIEPKPQTRPKFSKFGTYEDPKMKRWRKEVSGWIE
                     KNYDGPFFDDCIKVEVTFYMKAPKTLSKEPTQRSKGKTIQIYQNFVRELIWHAKKPDI
                     DNLIKAVFDSISDAGYDRIQKSGIVWSDDNIVCDLRAKKKYSQNPRIKVRIEEIDR"
     gene            4103..4450
                     /locus_tag="VBD27_001717"
     CDS             4103..4450
                     /locus_tag="VBD27_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025706.1"
                     /translation="MTDELINQFYKIFDNGIVRQIKKLDVDCKKAEQIRCSVTNNKRR
                     KTLPRPYVIEAFKDYFDEDTYVQMYLKSYREYHDPNDHETELFVKLKRAHKDTKLEHY
                     KQTKRLIYAAMSF"
     gene            4462..4764
                     /locus_tag="VBD27_001718"
     CDS             4462..4764
                     /locus_tag="VBD27_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HEO7025707.1"
                     /translation="MTDKINAETMQVAYNENYQTFLAKNADYGNSFEKSLNDFGYIAG
                     IVRIGDKYNRLYNLISSDKNVSESLSDTLNDMANYCVMLAVWLEEEERHRNLEHGG"
     gene            4899..>5071
                     /locus_tag="VBD27_001719"
     CDS             4899..>5071
                     /locus_tag="VBD27_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025708.1"
                     /translation="MIPNFRAFNKETKKMYGVDGFELSVRKIYRCSLADDEFRCGRLE
                     TFHFVEDNFDDYIL"
BASE COUNT         1852 a          855 c         1095 g         1269 t
ORIGIN      
        1 agtgctaacg cacactaaca aataacaatt accaataaat aacaaactta accttataga
       61 ataaataaaa gagtatttca tactcacagg aggacgaata gtgctaaata aattgaaaca
      121 atttttagga tttgatgaag tggaaacaac tgaaactata tcattagata acactttgat
      181 tgaggttaga gtcttacaag ctgaaaaccg tgaactgaaa gaagttatca ggcaaaagaa
      241 cgctatattg agagaactct ctgaggaaaa catagaactc gggcgtgatc gtaggcgcta
      301 tgctgacaca gtcgcaaccc aaaaacgttt gattgatgct tatgaaagca tggtaggtta
      361 ggaggcagca catggacaga ggactatttg gcacctttgc ctatgaccgt gattacttgc
      421 agcctgagcc tgaacgtgaa gagcatgaat ctgatgaatg ggtgttcaga ggtggtcagt
      481 ggatttatgt gggagactat tagcatatgg aaagaccaca aaaagagccc atgacaatga
      541 gctcctagca aaatatttct aaggtgatta taccacaaag gagggtatat gacaaaacaa
      601 gagtacgaga tggtctggga tttggctagt gaccgtgaac ggtatgagat gatagatagg
      661 tctatcaagc aaggtaagtg ggcgtgtgtc cgctatgcta gacagttgag gaggaagtat
      721 gtatgacaaa ggcaatatcg tttccagagt tgcaacagaa aatgcagcta gaaaagaaaa
      781 aatcaaaaga tgttaaatat gcgtttagaa atgctgagga catctataca acgtttaaag
      841 aattgaaaag tgagtggtct gtcattgtga cagatgagct aattgagctt gcagggaaag
      901 tatttgtcag ggcaacagca gtagcttttt gtgatgagac caatgaaaag tatcaatcaa
      961 cagcttattc tgaattgagc ccagtgccag tatttaatac tcaaaagggg caaatcaagc
     1021 aaatgcaaga gccacaatgg acaggtgcag tcagttcata cgctagaaaa tatgctttac
     1081 agggtttgtt tgctattggt gaaaaagata ttgatgagta cccagtagag gaaaatcagc
     1141 aagcaccaca gcagcaagga caaaacaccc ggcagcctca acaagatcag atacagtatg
     1201 ttagcaatga acaatacaat caaatcttga atgctattaa acatctttca cagatggtta
     1261 acaagccatt cgatgagttg atcatcaaca ttcaaaagca ttatcaaata caagattttc
     1321 accaagtgcc agcagatcat tttacaaccg tgatgaacta cttacaagca tcaatggcaa
     1381 aagctcaagg cattagcgac tttaacgatt tataggagta cacacatgac aaaagatgta
     1441 actaacagca tcttaacaga gattgaagta aattataccc cagctgtcat taacattgat
     1501 tacaagacag ttgagcaaca acttaaagcg gttgttgata ggtatgcaaa ttatgaggtg
     1561 acagcaaata cctataaggc tgattatgat gagcgcacac gcctcaataa gttcaaagag
     1621 gctctagaaa cacgccgcaa agaaatcaag gcaaacatca acaacccata caaagagttt
     1681 gagaaacggt acaaggcgat cctagcacca cttgatgagg ttatcaacaa catcacagtt
     1741 ggactaaatg caatcgatga acatgagcgc ttgatgcgca ttgatgtcat ccgggcaact
     1801 tttgaggata agtgtatggt ggccggtctt gaaaaagata cttttgaaat caaatataac
     1861 gagtatagtc ttaaaaagta ttttaagaca ggtaaatttg aactcaagaa aacaacaatt
     1921 gatgaaatgg atgccttggt attagcagaa tttgatgcac ttgaagaata taaagccagt
     1981 aagcaagcca tcaaagaaca agctcaagag tacgatttgc cagctgatag ctatatcaga
     2041 catctggaag atggcaaatc acttgtggat gtgcttaaaa tcatgaaagg tgaccgcgat
     2101 gcccttgcct tacgaaaaga gcaacaagag gcgcaatcta aggcagatgc tgaccgcaaa
     2161 gcagagcttg agcgtatagc tcaagagacc gctaatacca atatcaaagc ggttgattct
     2221 gatacagggg aaatcatcga agattatcca ccagagcttg agagtggggg cagagggaca
     2281 aatagaccac taacccaaaa cacagcgcca gaagcgccta aatttgagac tagtaagcca
     2341 gtaaactatg acttgcgact aactttccca catggtaacc cacaagctaa atacttcaaa
     2401 gaactatttg aaaaaaatgg cgttacatct gaaatgctgt ttaacggtaa aacacaaaaa
     2461 gagcttacag gaggtacaca aaatgtcttt taacattaca gaactgattg aaaatgtaaa
     2521 aggatggtca gttgccaaag agcttgcaga tgctgaccct ataaagcaga tgcaaaagct
     2581 caacgaggag tggggcgaac taaacgctgg taaagctaag ggtgatacag caaaacttta
     2641 tgatagcatt ggtgatgttt tcgttgtcct tattatccta tctcaacaaa tgaaatttga
     2701 gaagattgag cgattgattg acccaactgt aaacggattt ggttattatc ccaaaaatga
     2761 ggtaacaaca gatttacttt tactctatgg aatgaaagaa atcggcatga ttgctaatcg
     2821 tatgattgac cttatccaca atccaggcat cattaacaca cgcacagcca ttcagtttca
     2881 tatccgtaat cttacaagca tactttacaa agtagccatc aacgaagata cagatattaa
     2941 atactgtttg caactagcct gggatgaaat caaaaaccga caaggtaaaa tgattgatgg
     3001 tgtctttgtc aaagatgagg acatcaagga ggttgatgat gatgaataac gttgttttag
     3061 taggtaggtt gacaagagat gtagagctaa agtacacaca aagcaatgtt gcagtagcaa
     3121 cctttacatt agctgtcaat cgcccattta agaatgaggc tggagagcgt gaggctgact
     3181 ttattaattg tgttatctgg agacaatcag cagaaaacct tgccaactgg gcaaaaaaag
     3241 gctcattgat tggtattaca ggtgcaattc aaacacgcaa ctatgaaaac caacaagggc
     3301 agcgcgtgta tgtgacagaa gttattgcta gcaatttcca attgttagaa agtcgtagta
     3361 gtcaacaaag taaccaaggg taccaagata atcatggagg ttatcagcaa caacaaagct
     3421 acggtaatca aggtggcaat tttagaaatt ctggaggtta tcaatcgccg tttggtaact
     3481 caaacccaat ggatatctcg gatgatgatc ttccattttg agtgtttcgc taaagcttcg
     3541 ctcagtctaa caggtgaaag tcccgtccta acaattaacc agttcggtta gctagctcaa
     3601 aacgaattgc attgttggta gggattgaag aggaatatga cttttaaaac agaatttgaa
     3661 ataccaatcg aaccaaaacc tcaaactaga cctaagttca gcaaatttgg tacgtacgaa
     3721 gatccaaaga tgaagagatg gcgaaaagag gtttctggat ggatagaaaa aaattatgat
     3781 ggaccgtttt tcgatgattg cataaaggta gaggtaacct tttacatgaa agcccccaaa
     3841 acgctatcaa aagagcctac acaacgttct aaaggtaaaa caatacaaat atatcagaac
     3901 ttcgtgcgtg agcttatatg gcacgctaag aagcctgata ttgataattt gattaaagct
     3961 gtttttgata gtatttccga tgcaggttac gacagaatac agaaatcagg tattgtgtgg
     4021 tcagatgaca atatcgtatg cgatttaaga gcaaagaaga aatacagtca gaatccaaga
     4081 ataaaagtga ggattgaaga gattgacaga tgaattaata aatcaatttt acaaaatttt
     4141 tgacaatggg attgtaaggc aaattaaaaa gctagatgta gattgtaaaa aagccgagca
     4201 aataagatgt agcgttacaa ataacaagcg tagaaaaaca ttgccacgcc cttatgtaat
     4261 tgaggcgttt aaagattatt ttgacgaaga cacttatgta cagatgtatc tcaaatcata
     4321 tcgtgagtat cacgatccaa atgaccatga aacagaactt tttgtaaaac taaaaagagc
     4381 gcacaaagat actaagttag agcattacaa acagactaag cgtctgatat atgcagctat
     4441 gagtttttag aggtataaca catgacagat aaaattaacg cagaaactat gcaagtagca
     4501 tataacgaaa actatcaaac atttttagcc aaaaacgcag attacggaaa ttctttcgag
     4561 aagtctctaa acgacttcgg atacatcgct ggtatcgttc gtataggcga taaatacaac
     4621 agactatata atcttataag cagcgacaaa aacgtctcag aaagcctgtc agacacgtta
     4681 aacgacatgg ctaattattg cgtgatgtta gcggtttggt tggaggaaga ggaacgacac
     4741 cgaaatttag agcatggggg ataaccgatg ttaattaata aaaatttagt aagagacatt
     4801 agacttgcta tcaatgaata cgatgataat tatgacgaag ataacgcaga agtttgtata
     4861 gaagtcatta gacgattgat tgaacaggag gaattctgat gataccgaat tttagagcgt
     4921 ttaataaaga gaccaaaaag atgtatggtg ttgacggctt tgaattaagt gtgcgcaaaa
     4981 tatatagatg cagcttagca gatgatgagt ttcgttgtgg tcgcttagag acgtttcatt
     5041 ttgtcgagga taactttgac gattatatcc t
//
LOCUS       DATRQA010000131         4951 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.131, whole genome shotgun sequence.
ACCESSION   DATRQA010000131 DATRQA010000000
VERSION     DATRQA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.131"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            116..1840
                     /locus_tag="VBD27_001720"
     CDS             116..1840
                     /locus_tag="VBD27_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEO7025709.1"
                     /translation="MNNNEKKVKYFLRKTAYGLASMSAAFIVCSGIVNTPTVSADSPD
                     TLKVEKLGKLKDVKSVHELTPISIPNELKGAKEQALSSIISHPNITNSEVDKLASDYS
                     FRINTSNDVNDVKRLLNEFYNAVARKQLDTNSADYRSKIDNISTTGLAIALEAKEIYE
                     ANKSILPHRYKDSVGTYVNSFEERRSPGKFNIWNGQEGFNAAQKLLEDVKKLLLELQN
                     LTKNNKPNIQVPKQAPTEAAKPALSPEALTRLTTWYNQAKDLLKDDQVKDKYVDILSV
                     QKAVDQAYDHVEEGKFITTDQANQLANKLRDALQSLELKDKKVAKPVAKGTYDVKYVD
                     TEGKEVAKSRHFEGEEGAAFVTSAKEVAGYKLVRTEGAVSNVFTAGAQVRTYVYEKVA
                     KPVAKGTYDVKYVDTEGKEVAKSRHFEGEEGAAFVTSAKEVAGYKLVRTEGAVSNVFT
                     AGAQVRTYVYEKVKPEVKPDVKPEAKPEAKPEVKPDVKPEAKPEAKPEVKSDVKPEAK
                     PEAKPATKKSVNTSGNLVAKKAIENKKYSKKLPSTGEAASPLLAIVSLIVMLSAGLIT
                     IVLKHKKN"
     gene            2046..2585
                     /gene="nusG"
                     /locus_tag="VBD27_001721"
     CDS             2046..2585
                     /gene="nusG"
                     /locus_tag="VBD27_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO7025710.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGKTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRSILISMGQTVDVFDTNIKEGDVVQIIDGAFIGQEGRVVEIENNKVKLMI
                     NMFGSETQAELELYQVAEL"
     gene            complement(2632..3837)
                     /locus_tag="VBD27_001722"
     CDS             complement(2632..3837)
                     /locus_tag="VBD27_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 8 glycosyl transferase"
                     /protein_id="HEO7025711.1"
                     /translation="MEKAVALAVDSNYLDKALVTIKSICVYNRNITFYLFNQDTPVEW
                     VRNINRKLEPLGSKLINVKIYNYDIAHLTTFLTVSTWFRLFLADYIPSSRVLYLDSDI
                     IVNTNLDYLFELDFKGHYLAAVKDPHKNEEGGFNAGMLLANLELWREDGLTKTLLKTA
                     EELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDVVSRYNHRSYLYLNIENRTPNI
                     IHFLTSDKPWNENSVARFRELWWYYFQLDFCQLTGKQRKVISYEKSMELLSVSDIHLF
                     TLTSSDNLEHIESLICRCPTVQFHIGAYTTVSNKLSKLEQYPNVLVYPELIEARIEKL
                     ITLATAYLDINHGPEVGNILQRVHLKQKPIYSFNNTSHQENITKHIVNHNSIDNMVVL
                     INELNLNQL"
     gene            complement(3902..>4951)
                     /locus_tag="VBD27_001723"
     CDS             complement(3902..>4951)
                     /locus_tag="VBD27_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEO7025712.1"
                     /translation="MKDRVGQTGNQIQDVKLFHDHLSPKWENKKLNHINYMTYARYFI
                     PQYISADTVLYLDSDLVVTTNLDNLFQISLDNAYLAAVPALFGLGYGFNAGVMVINNQ
                     RWRQENMTIKLIEKNQKEIENANEGDQTILNRMFENQVIYLDDTYNFQIGFDMGAAID
                     GHKFIFDIPITPLPKIIHYISGIKPWQTLSNMRLREVWWHYNLLEWSSIISSKKVFGL
                     DHPIKTQNYRLNFLIVTTSDCIPSISELVTALPDCLFHIAAPTVMSDILISLLAFENI
                     RLHKTYTPPIIDKLLAEADIYLDINVFGKMEGILQKMSQSEKPILTTRECNSQLVPET
                     VFASIDDLINYLQSI"
BASE COUNT         1761 a          758 c          902 g         1530 t
ORIGIN      
        1 tgaaataaaa aactccaagt tatataatta gttaaggaac tcaaattaaa ttaaaataaa
       61 cgtggtccta tcctaataaa ttaggatttt taactaacta tataagggag cgaatatgaa
      121 taataacgaa aaaaaagtaa aatacttttt aagaaaaaca gcttatggtt tggcttcaat
      181 gtcagcggcg tttatagtat gtagtggtat tgtaaatact cctacagtgt ctgctgatag
      241 tcctgataca ttaaaagtcg aaaaattagg caaattgaaa gatgtgaaat cagttcatga
      301 actcacaccc atatcaatac cgaacgaatt aaaaggtgct aaagagcaag cactttcttc
      361 aataatttca catcctaata taactaattc ggaagtagac aaactagcta gtgactatag
      421 ttttagaatt aatacatcta atgatgtgaa cgacgttaaa cgtctattaa atgaatttta
      481 taacgcagtt gcaaggaaac agttagatac aaattctgct gactaccgta gtaaaattga
      541 taatatcagt actacaggtc ttgcgatagc tcttgaggct aaagaaattt atgaagcaaa
      601 taaatctata ttacctcatc gttacaaaga ttctgttgga acttatgtga acagttttga
      661 ggaaagacga agtccaggaa aatttaatat ttggaatggt caggaaggat ttaatgcagc
      721 tcaaaaattg ttagaagatg ttaaaaaatt attacttgag ctacaaaatt taacaaaaaa
      781 taacaaacca aatattcaag tacctaaaca agcacctaca gaagctgcaa aaccagcttt
      841 gtcaccagaa gccttgacaa gattgactac atggtataat caagctaaag atctgcttaa
      901 agatgatcaa gtaaaggaca aatacgtaga tatactttca gttcaaaaag ctgttgacca
      961 agcttatgat catgtggaag agggaaaatt tattaccact gatcaagcaa atcaattagc
     1021 taacaagcta cgtgatgctt tacaaagttt agaattaaaa gataaaaaag tagccaaacc
     1081 agtagctaaa ggtacatacg atgttaagta tgtagacaca gaaggaaaag aagtagctaa
     1141 gtcacgtcac ttcgaaggag aagaaggcgc agcttttgtc acttcagcga aagaagtagc
     1201 gggttacaaa cttgttagaa cggaaggcgc tgtttcaaat gtcttcacag caggagcaca
     1261 agtacgtaca tatgtttacg aaaaagtagc caaaccagta gctaaaggta catacgatgt
     1321 taagtatgta gacacagaag gaaaagaagt agctaagtcg cgtcacttcg aaggagaaga
     1381 aggcgcagct tttgtcactt cagcgaaaga agtagcgggt tacaaacttg ttagaacgga
     1441 aggtgctgtt tcaaatgtct tcacagcagg agcacaagta cgtacatatg tttacgaaaa
     1501 agttaaacca gaagttaaac cagacgttaa gccagaggcc aaaccagagg ctaagccaga
     1561 agttaaacca gacgttaagc cagaggccaa accagaggct aagccagaag ttaaatcaga
     1621 cgttaagcca gaggctaagc cagaagccaa gccagcaacc aaaaaatcgg ttaatactag
     1681 cggaaacttg gtggctaaaa aagctattga aaacaaaaag tatagtaaaa aattaccatc
     1741 aacgggtgaa gccgcaagtc cactcttagc aattgtatca ctaattgtta tgttaagtgc
     1801 aggtcttatt acgatagttt taaagcataa aaaaaattaa atttattttt aaacataaat
     1861 cgaaaggtct atcctttcaa aatcttaagg caaacctcat cagaactttg ccttttttat
     1921 ttgaatttta gaatgattta gtcggtactt taattgacag cttattttat tttctttata
     1981 atatatattg aaaatagcag tccaataagc cgtaaaaggc ttttttatta aggaaaggaa
     2041 aacttatgtt agattctttt gataagggtt ggtttgtact ccaaacctac tcaggttatg
     2101 aaaataaagt aaaggaaaat ctcttgcaac gtgcgcaaac ttataatatg ttagacaaca
     2161 ttttgcgtgt ggaaatccct actcaaactg taaatgttga gaaaaatggg aaaacaaaag
     2221 aaattgaaga aaatcgcttc ccaggttatg tacttgtgga aatggtaatg actgatgaag
     2281 cctggtttgt tgttcgtaat actccaaacg ttactggatt tgttggatca cacggcaatc
     2341 gctctaaacc aacaccgttg ttagaagaag agattcgttc aatactcatc tcaatgggac
     2401 aaaccgttga tgtctttgat actaatatca aggaaggtga tgttgttcaa atcattgatg
     2461 gtgctttcat cggacaagaa ggacgtgtag ttgagattga gaataacaaa gttaaattaa
     2521 tgatcaacat gttcggctca gaaacacaag cagaacttga gttgtaccaa gttgcagaat
     2581 tataaaactt ctgaaaaatc catcttaaaa taaagatgga tttttgttaa tttataattg
     2641 atttaggttt aattcgttaa ttaaaacaac catattatca atagaattat gattaacgat
     2701 atgtttggtt atattctctt gatgagacgt gttgttgaaa gaataaattg gcttttgttt
     2761 caaatgaact ctttgtaaaa tattacctac ttctggacca tgattaatat caaggtatgc
     2821 agtggctaat gttattaatt tttcaattct tgcctcaatt aattcagggt aaactaggac
     2881 atttggatat tgttctagtt tgctaagttt atttgacact gttgtgtagg caccaatatg
     2941 gaattgaaca gtaggacatc tacaaattag cgattcaatg tgttccaaat tatcggaaga
     3001 tgtaagggtg aaaagatgaa tatctgaaac agaaagcaat tccatggact tttcgtaaga
     3061 aatcactttt ctttgcttgc cggttaattg gcaaaaatca agttggaagt aataccacca
     3121 tagttctcta aaccttgcaa cgctattttc attccaaggt ttgtcactag ttaaaaaatg
     3181 tataatatta ggagttctat tttctatgtt tagatataaa taagatcgat gattatagcg
     3241 gctaacgaca tcataagttt gaaagttcca tgttttgttc agagataacc aacgattatg
     3301 gcaaacaatg ttcaagatac tttgatcccc tgttttgaca actcggtgga gttcttcagc
     3361 tgtttttagt aatgttttag tgaggccatc ttcccgccat agttctagat tagctaaaag
     3421 catgccagca ttaaaccctc cttcttcatt tttatgggga tctttgacgg ctgctaagta
     3481 atgaccttta aaatctagtt caaataagta atcaagatta gtgttaacga taatatctga
     3541 atctaaataa agtacacgtg aactaggtat ataatctgct aaaaataatc taaaccatgt
     3601 actaacagtt agaaaagtcg ttagatgagc aatatcatag ttatatattt taacattaat
     3661 cagttttgat cctagaggct ctagtttcct gtttatatta cgtacccatt caactggggt
     3721 atcttgattg aataaataaa aagttatatt tctattataa acacaaatag actttattgt
     3781 tactaaggct ttatccaagt agtttgaatc aactgcaagt gcaactgctt tttccattta
     3841 aattctccta gttaagtgtt tagaataatg ttgggtatat agtgaatgga tgccaatttt
     3901 tttatataga ctgaagataa ttaattaaat catcgataga tgcaaaaact gtttctggaa
     3961 caagctggct attgcattct cttgtagtaa gaattggttt ttcagattgt gacattttct
     4021 gtagaatgcc ctccattttt ccaaaaacat taatatcaag ataaatatca gcttctgcta
     4081 ataatttatc aataataggt ggagtgtaag ttttatgaag gcggatattt tcaaaagcta
     4141 ataggcttat aagaatgtca gacataactg ttggtgctgc aatgtgaaat agacaatctg
     4201 gaagggcagt gactaattct gagatagatg gtatacaatc agaagttgta acaataagga
     4261 aattgagacg ataattttgt gttttaattg ggtggtctaa accaaatact tttttactag
     4321 atatgatact tgaccattca agtaaattat agtgccacca tacctcacgg agtctcatat
     4381 ttgataatgt ttgccaaggt ttgattcccg aaatgtagtg aataattttt gggagtgggg
     4441 taattgggat gtcaaaaata aatttatgcc catcgatagc agctcccata tcaaaaccaa
     4501 tttgaaaatt gtaggtatca tctaaataaa ttacctgatt ttcaaacatg cgattaagaa
     4561 ttgtttgatc cccttcgttg gcattctcaa tttccttttg atttttttca attaatttaa
     4621 tagtcatatt ttcttgtcgc caacgttggt tgttaattac cattactcca gcattaaacc
     4681 catatccaag cccaaaaaga gctggaactg cagctaaata tgcattgtct agtgaaattt
     4741 gaaagaggtt atctaaatta gtagtaacaa ctaagtcaga gtcaagatat aaaactgtat
     4801 cagctgagat gtactgaggt atgaaataac gagcataggt catataatta atatgattaa
     4861 gctttttatt ttcccatttt ggggataagt gatcatggaa gagctttaca tcctgaattt
     4921 gatttccagt ttgtccaacc ctatctttca t
//
LOCUS       DATRQA010000132         4816 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.132, whole genome shotgun sequence.
ACCESSION   DATRQA010000132 DATRQA010000000
VERSION     DATRQA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.132"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            57..1214
                     /locus_tag="VBD27_001724"
     CDS             57..1214
                     /locus_tag="VBD27_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO7025713.1"
                     /translation="MNKLKVNSVVERKIKSGAQLLEKKDFDTSLVNQLVQLFSQSNQF
                     LGMAYLSPQNKGIGWLLSRQIFDFNHVYFVSLFEKSREKRQKFEKSSQTTAYRLFNQD
                     GDNFGGLTIDFYSDYALFSWYNEFVYTNRQMIVAAFKQVYPNIKGAYEKIRFKGLDFE
                     SAHLYGQEAPESFLILENNIKYSVFLNDGLMTGIFLDQHDVRKALATNLSEGKKVLNM
                     FSYTAAFSVAAAVGGALETTSVDLAKRSRELSKAHFDANQIVTDNHRFIVMDVFEYYK
                     YAKRKHLSYDVIVIDPPSFARNKKQTFSVTKDYYKLIEQALDILAPGGTIIASTNAAN
                     LTVSQFKKQLEKGFGKASHNYISLQQLPEDFTVNDKDQQSNYLKVFTIKVK"
     gene            1214..1891
                     /locus_tag="VBD27_001725"
     CDS             1214..1891
                     /locus_tag="VBD27_001725"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEO7025714.1"
                     /translation="MKIVVPVMPRSLEEAQEIDLSKFDSVDIIEWRADALPKDDIINV
                     APAIFEKFAGHEIIFTLRTTREGGNIVLSDAEYVELIQKINSIYNPDYIDFEYFSHKE
                     VFQEMLEFPNLVLSYHNFQETPENIMEIFSELTALAPRVVKIAVMPKNEQDVLDVMNY
                     TRGFKTINPDQVYATVSMSKIGRISRFAGDVTGSSWTFAYLDSSIAPGQITISEMKRV
                     KALLDAD"
     gene            1985..3052
                     /locus_tag="VBD27_001726"
     CDS             1985..3052
                     /locus_tag="VBD27_001726"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO7025715.1"
                     /translation="MQVEVDLPNHPYHIKIEEGCFSEAGDWVSHLWQKQMITIITDSN
                     VEILYGESLVNQLKKQGFTVHVFSFAAGEASKTLEVANRIYGFLAKHHMTRSDGIIAL
                     GGGVVGDLAAFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTSFAKNMVGTFAQP
                     DGVLIDPVTLKTLGNRELVEGMGEVIKYGLIDDIKLWHILEEMDGSIDSILDNALAII
                     YHSCQVKRKHVLADQYDKGLRMHLNFGHTIGHAIEVHAGYGEIMHGEAVAIGMIQLSR
                     VAERKNLMPRGISQDIYNMCLKFGLPVHYAEWDKDVLFNILSHDKKASGQFIKIVILP
                     QLGSATVHQIPLEEMRDYLEK"
     gene            3053..4219
                     /gene="aroC"
                     /locus_tag="VBD27_001727"
     CDS             3053..4219
                     /gene="aroC"
                     /locus_tag="VBD27_001727"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO7025716.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLKLSAKDINEDLKRRQGGYG
                     RGNRMKIETDQVIISSGVRHGKTLGSPITLTVTNKDHSKWLDIMSVEDIEERLKQKRR
                     IKHPRPGHADLVGGIKYRFDDLRNALERSSARETTMRVAIGAIAKRILKEIGIEIANH
                     IVVFGGKEITVPDKLTVQQIKVLSSQSQVAIVNPSFEQEIKDYIDSVKKAGDTIGGVI
                     ETIVGGVPVGLGSYVHWDRKLDAKIAQAVVSINAFKGVEFGLGFKSGFLKGSQVMDSI
                     SWTKDQGYIRQSNNLGGFEGGMTNGEPIVVRGVMKPIPTLYKPLMSVDIDTHEPYRAT
                     VERSDPTALPAAGVVMEAVVATVLVTEVLEKFSSDNMYELKEAVKLYRNYVNHF"
     gene            4303..4638
                     /locus_tag="VBD27_001728"
     CDS             4303..4638
                     /locus_tag="VBD27_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEO7025717.1"
                     /translation="MANVYDLANELERAVRALPEYQAVLTAKAAIENDADAQVLWQDF
                     LATQSKVQEMMQSGQMPSQEEQDEMSKLGEKIESNDLLKVYFDQQQRLSVYMSDIEKI
                     VFAPMQDLM"
BASE COUNT         1584 a          745 c          979 g         1508 t
ORIGIN      
        1 taaagaaagg tcgaattcag tatgaaaaaa gataaaaatt tgttataata ctgtttatga
       61 ataaacttaa agtgaattct gtcgttgaaa gaaaaatcaa atcaggtgct cagttactgg
      121 aaaaaaaaga ttttgatacc agtttagtta accagttggt tcaacttttt tcacagtcaa
      181 atcaattctt agggatggcc tatctttcac cacaaaataa agggattggt tggttactat
      241 caagacaaat ttttgatttt aaccatgttt actttgtatc gctattcgaa aaatctagag
      301 agaagcgtca aaaatttgaa aaatctagcc aaacaacagc ctaccgcttg tttaatcagg
      361 atggagataa ctttggtggc ctaactatag atttttatag tgactatgct cttttttcgt
      421 ggtacaatga atttgtttat actaatcgac aaatgattgt tgcagccttt aagcaggtct
      481 atcctaatat taaaggggca tatgaaaaaa ttcgtttcaa aggtttagac tttgaaagtg
      541 ctcatttgta cggtcaagag gctcctgaat catttttgat tttagaaaat aatatcaaat
      601 atagtgtctt tttgaatgat gggttaatga caggtatttt ccttgaccaa catgatgtca
      661 gaaaagcctt agcaactaat ctatcagaag gtaaaaaagt tctaaatatg ttttcataca
      721 ctgcggcttt ttctgtagct gcagctgttg gaggagcatt agagacaact tctgttgatt
      781 tagcaaaacg ctcgcgtgaa ctttcaaaag cacactttga tgctaatcag attgtcacag
      841 ataaccatcg atttatcgtt atggatgttt ttgaatatta taagtatgcc aaaagaaaac
      901 atctatcata tgatgtgatt gttattgatc caccaagttt tgctcgaaat aaaaaacaaa
      961 ctttttcggt tactaaagat tactataaat taattgaaca agctttagat attttagccc
     1021 ctggtggaac tatcattgca tcaactaacg cagctaatct aaccgtatca caatttaaga
     1081 aacaattgga aaaggggttt ggtaaagctt cacataatta cattagctta cagcagttgc
     1141 ctgaagattt cactgtaaat gataaggacc aacaaagtaa ttatttaaaa gtatttacaa
     1201 taaaggtaaa ataatgaaaa tagtagtacc agtaatgcct cgcagtcttg aagaggctca
     1261 agaaatagat ttatcaaaat ttgatagtgt tgatattatt gaatggcgag ctgatgcctt
     1321 accaaaggat gacattatta atgtagctcc agctattttt gagaaattcg caggtcatga
     1381 aattattttt actcttcgta caacgcgtga aggtggtaat attgtcttat ctgatgctga
     1441 gtatgttgag ttaatccaga aaattaattc tatctacaat ccagattata ttgattttga
     1501 gtatttttca cataaagaag tttttcaaga aatgctagaa tttccaaatt tagtcctgtc
     1561 ttatcacaat tttcaagaga caccggagaa tattatggag atattttcag aattaacagc
     1621 cctagcacca cgagttgtga aaatcgcagt aatgccaaag aatgaacaag atgtcttaga
     1681 cgttatgaat tacactcgcg gtttcaagac tattaatcct gatcaagttt atgcgacggt
     1741 atctatgagt aaaattggac gtatttctcg ttttgctggt gatgtaactg gatctagttg
     1801 gacatttgca tatttagatt catctatcgc acccggacaa attactattt cagagatgaa
     1861 gcgtgtcaaa gcattgcttg acgctgactg atacgagata acaaaatgtt aaatatactg
     1921 atgagatccc tagcaattgc ttagggtttt tgtcttatta aaacaaacat gagaggacat
     1981 tagaatgcag gttgaagttg acttaccgaa tcacccttat catattaaaa ttgaagaagg
     2041 atgtttttct gaggctggtg actgggtatc acatttatgg cagaaacaaa tgataactat
     2101 tattacagat agtaatgtag aaatattata cggggagtcg cttgtcaacc agttaaaaaa
     2161 acaaggtttt acagtacatg tttttagttt tgctgcgggt gaagctagta aaactttgga
     2221 agttgctaat cgtatttatg gttttttagc aaagcatcat atgaccagaa gtgatgggat
     2281 cattgcactt ggtggaggcg tcgtcggaga tttagcagcg tttgttgctt ctacttatat
     2341 gcggggaatc cattttcttc aaattccaac tagtttaaca gcacaggttg attcttccat
     2401 tggcggaaaa acaggagtca acacttcatt cgctaaaaat atggtaggta cttttgcaca
     2461 acctgatggt gtactgatag atcctgttac tctaaaaaca ttgggcaatc gtgaattagt
     2521 tgaaggtatg ggagaagtca tcaaatatgg cttgattgat gatataaaac tctggcatat
     2581 cttagaagag atggatgggt ctattgatag tatccttgat aatgcattag ctattattta
     2641 tcactcttgt caagttaaac gaaagcatgt tttagctgat caatatgata aagggttaag
     2701 gatgcattta aattttggcc acactatcgg tcatgctatc gaagtacatg ctggctatgg
     2761 agaaataatg catggagaag ctgttgcaat tggaatgatc cagctttctc gtgtggctga
     2821 gcgtaaaaat cttatgccaa gaggtatcag tcaagatatt tataatatgt gtttaaaatt
     2881 tggccttccg gtccactatg cagaatggga taaggacgtc ttatttaaca tattgagtca
     2941 tgataaaaaa gcaagtggac aatttattaa aattgttata ctgcctcaat taggtagtgc
     3001 aacagtccac caaatacctt tagaagaaat gagagattat ttggagaaat agatgagata
     3061 tttaactgct ggtgagtcac atggacctag tttaacagct attatcgaag ggattcctgc
     3121 gggactgaaa ttatctgcta aagatattaa tgaagattta aaacgccgcc aaggtggtta
     3181 tggtcgtggg aatcgaatga aaattgaaac tgatcaagtg atcattagtt ctggtgtgcg
     3241 ccatggaaaa accttgggtt cacctattac actgacagta actaataagg atcatagcaa
     3301 atggctggac atcatgtcag tcgaagacat tgaagagcgt ctaaagcaaa aacgtcgtat
     3361 taagcatcct agacctgggc acgcagattt agtaggtgga atcaaatacc gatttgatga
     3421 tttgagaaat gctttagagc gttcttcggc tcgtgagacc acaatgcgag tagctattgg
     3481 tgcgattgct aaacgtatct tgaaagaaat tggtatcgaa attgctaacc atattgtagt
     3541 tttcggtggc aaggagatta ctgtccctga taagctaaca gttcaacaaa tcaaagtgtt
     3601 atctagtcaa tcgcaagtag ctatcgtcaa tccatcattt gaacaagaaa ttaaggatta
     3661 tattgacagt gtgaaaaagg ctggagatac tatcggagga gtgattgaaa ctatcgttgg
     3721 aggagttccc gttggtttag gctcttatgt acactgggat agaaagttag atgctaagat
     3781 agcgcaagca gttgtatcta tcaatgcttt taaaggtgtt gaatttggtc taggttttaa
     3841 gtcagggttt ttaaaaggaa gtcaagtaat ggactccata tcatggacaa aggaccaagg
     3901 ttacatacgt cagtcaaata atttaggcgg ttttgaaggc ggtatgacta atggagagcc
     3961 tattgtcgtt aggggagtaa tgaagccaat tcctacgctt tacaagccac taatgtcagt
     4021 tgatattgat actcacgagc cttatagagc tacagtggaa cgttctgatc ccacggcact
     4081 tccggctgca ggtgtcgtta tggaagctgt agtagccacc gtcctggtga cagaagtgct
     4141 agaaaaattt tcttcagaca acatgtatga actaaaagaa gctgtaaaac tatatcgcaa
     4201 ttatgttaat catttttaag tgaaaactag tagcattcaa ttgcaaaaaa aggtataaaa
     4261 gtctacaata gtggtaagtt caagtagaaa aaggagtaac ttatggctaa tgtatatgat
     4321 ttagcaaatg aattagaacg tgctgttcgt gctttaccag aataccaagc agttttaact
     4381 gcaaaagcag ctattgaaaa tgatgcggat gcacaagtgc tttggcaaga ctttttggct
     4441 acccaatcaa aagttcaaga aatgatgcaa tctggccaaa tgccaagtca agaagaacaa
     4501 gatgaaatgt ctaaacttgg ggaaaaaatt gaatccaatg accttttaaa agtttatttt
     4561 gaccaacaac aacggttgtc tgtctatatg tctgatatcg aaaaaattgt ctttgcaccc
     4621 atgcaggact tgatgtaata gtataaaaga gagagctcct tgagttttct cttttatgct
     4681 atttacattc agagcatcct aattgctgcg cttttacatt attgcattat cctataaaaa
     4741 tgtattttta tacttttttt gagagcggct atttattttt agacttagtc atgtctctaa
     4801 atacccctag ttagtg
//
LOCUS       DATRQA010000133         4803 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.133, whole genome shotgun sequence.
ACCESSION   DATRQA010000133 DATRQA010000000
VERSION     DATRQA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.133"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            81..1034
                     /locus_tag="VBD27_001729"
     CDS             81..1034
                     /locus_tag="VBD27_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEO7025718.1"
                     /translation="MNTIYNTLRTDKGYKVYEGYLYEITGEECEEALDLVIPKNIVFA
                     DTDTCGYTFLLNEDGTVYDDVTFYKFDDKYWLASHKALDSYLDNINFDYTVTDISDEY
                     KMLQIEGRYSGEIAQSFYEYDISTLNFRTLIEMTYKGEKGYLARFGFSGEFGYQFFLP
                     SSIFATFVSDVCEGIAEYGDELDRYLRFEVGQPITDIYQQEEYSLYEIGYSWNLDFTK
                     EEFRGRDSLLEHIRSATVKSVGFSTKEKLASGTPVLFDDQIVGKIFWIADEKDSSENY
                     LGLMIVNQTYAHSGVTFVTEDGQILKTQSSPYCIPESWNKE"
     gene            1031..3226
                     /locus_tag="VBD27_001730"
     CDS             1031..3226
                     /locus_tag="VBD27_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO7025719.1"
                     /translation="MSVYVSGIGIISSLGKNYSEHKQHLFDLKEGISKHLYKNHDSIL
                     ESYTGSITSDPEVPEQYKDETRNFKFAFTAFEEALASSGVNLKAYHNIAVCLGTSLGG
                     KSAGQNALYQFEEGERQVDASLLEKASVYHIADELMAYHDIVGASYVISTACSASNNA
                     VILGTQLLQDGDCDLAICGGCDELSDISLAGFTSLGAINTEMACQPYSSGKGINLGEG
                     AGFVVLVKDQSLAKYGKIIGGLITSDGYHITAPKPTGEGAAQIAKQLVTQAGIDYSEI
                     DYINGHGTGTQANDKMEKNMYGKFFPTTTLISSTKGQTGHTLGAAGIIELINCLAAIE
                     EQTVPATKNEIGIEGFPENFVYHQKREYPIRNALNFSFAFGGNNSGVLLSSLDSPLET
                     LPARENLKMAILSSVASISKNESLSITYEKVASNFNDFEALRFKGARPPKTVNPAQFR
                     KMDDFSKMVAVTTAQALIESNINLKKQDTSKVGIVFTTLSGPVEVVEGIEKQITTEGY
                     AHVSASRFPFTVMNAAAGMLSIIFKITGPLSVISTNSGALDGIQYAKEMMRNDNLDYV
                     ILVSANQWTDMSFMWWQQLNYDSQMFVGSDYCSAQVLSRQALDNSPIILGSKQLKYSH
                     KTFTDVMTIFDAALQNLLSDLGLTIKDIKGFVWNERKKAVSSDYDFLANLSEYYNMPN
                     LASGQFGFSSNGAGEELDYTVNESIEKGYYLVLSYSIFGGISFAIIEKR"
     gene            3231..4442
                     /locus_tag="VBD27_001731"
     CDS             3231..4442
                     /locus_tag="VBD27_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylJ protein"
                     /protein_id="HEO7025720.1"
                     /translation="MSKFLFAPFMMNLGESQRLSKLANCLYEQGHDIHILGDNYYPFL
                     FNNDAYRYHHCVEDKSVYNSKRYDAFFSLSTDFNFLSEEEIERICSIERDLLRQEKFD
                     AVLTGYRLSIVTSCRLESIPLIWIISGATHISEIVENSEGILPNGKISKASKPQTKDF
                     IKRVITTYSTNVKTWNNYIKKYGGKPFNNALELFTGDLNLVTDYSLFYEFDKDSSYKT
                     IGPILIDNVGFSKCSQINQDNKTVLLSFGTSFKRDWVESFLKTLPRHYHYLLTSCGEA
                     INIPGSYIEIVDFIDFKTIDKEIYFAIIHGGQGTVYAMAAQGIPFIGIPFFNEQFWNI
                     KKFSKEKCAYLVKKPDVREIRKAISCLERDYDIYKSYMIRLSQGIKEEAEASLQKAEA
                     EIESFIRKRQS"
     gene            4411..>4803
                     /locus_tag="VBD27_001732"
     CDS             4411..>4803
                     /locus_tag="VBD27_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylK protein"
                     /protein_id="HEO7025721.1"
                     /translation="MNPLSEKDKVNYIMIYKNRQSTLENNSSGDRLMRYQQANRQVSK
                     DKLLTGSYFVYQCLKKLGFTNKICQESFSSVSSYLIGLPKGKISYSNSGDYHILTYAP
                     SGSTGVDIEKYKDRSEQTYQNYLGESVSS"
BASE COUNT         1621 a          694 c          947 g         1541 t
ORIGIN      
        1 aaaataaggt gcatgtttaa aaggaactca ccttatgcta caaatgtgag cattacagaa
       61 tatagaaagt gtgaagaaaa atgaatacta tttataatac attgagaaca gataaaggtt
      121 ataaagttta tgaggggtat ttatatgaaa ttactggtga agaatgtgaa gaagccttag
      181 accttgtgat tcctaagaat attgtatttg cagatacaga tacttgtggc tacacttttt
      241 tactcaatga agatggaaca gtttatgatg atgtgacttt ctacaaattt gatgataaat
      301 attggttggc tagtcataaa gctttggatt cttatttaga caacatcaat tttgactata
      361 ccgtaacaga tatttctgac gagtataaaa tgctgcaaat tgaaggaaga tattcgggag
      421 aaattgctca gtcattttat gaatatgata tttcaacact taattttcgt actttgatag
      481 agatgactta taaaggtgag aaaggttatc ttgctagatt tggtttttct ggagaatttg
      541 gctatcaatt tttcctacca tcttctattt ttgctacttt tgtttcggat gtctgtgaag
      601 gtatagcaga gtatggggat gaacttgata gatatttaag gtttgaagtg ggacaaccca
      661 ttactgatat ttatcaacaa gaagaatatt ctttatatga aataggttat tcttggaatc
      721 tagatttcac aaaggaagaa tttagaggtc gcgatagctt gttagagcac atcagatcag
      781 caacagttaa aagtgttgga ttctcaacga aggaaaaact cgcttcagga acaccagtgc
      841 tatttgatga ccaaattgtt ggaaagattt tttggatagc agacgagaaa gactcttcgg
      901 aaaattacct aggtttgatg attgttaacc aaacatatgc tcattcagga gttacttttg
      961 taacagaaga tggccaaatt ttgaaaacac aatcaagccc ttattgtatc ccagaaagtt
     1021 ggaacaaaga atgagcgtat atgttagtgg aataggaatt atttcttctt tgggaaagaa
     1081 ttatagcgag cataaacagc atctcttcga cttaaaagaa ggaatttcta aacatttata
     1141 taaaaatcac gactctattt tagaatctta tacaggaagc ataactagtg acccagaggt
     1201 tcctgagcaa tacaaagatg agacacgtaa ttttaaattt gcttttaccg cttttgaaga
     1261 ggctcttgct tcttcaggtg ttaatttaaa agcttatcat aatattgctg tgtgtttagg
     1321 gacctcactt gggggaaaga gtgctggtca aaatgccttg tatcaatttg aagaaggaga
     1381 gcgtcaagta gatgctagtt tattagaaaa agcatctgtt taccatattg ctgatgaatt
     1441 gatggcttat catgatattg tgggagcttc gtatgttatt tcaaccgcct gttctgcaag
     1501 taataatgcc gtaatattag gaacacaatt acttcaagat ggcgattgtg atttagctat
     1561 ttgtggtggc tgtgatgagt taagtgatat ttctttagca ggcttcacat cactaggagc
     1621 tattaataca gaaatggcat gtcagcccta ttcttctgga aaaggaatca atttgggtga
     1681 gggcgctggt tttgttgttc ttgtcaaaga tcagtcctta gctaaatatg gaaaaattat
     1741 cggtggtctt attacttcag atggttatca tataacagca cctaagccaa caggtgaagg
     1801 ggcggcacag attgcaaagc agctagtgac tcaagcaggt attgactaca gtgagattga
     1861 ctatattaac ggtcacggta caggtactca agctaatgat aaaatggaaa aaaatatgta
     1921 tggtaagttt ttcccgacaa cgacattgat cagcagtacc aaggggcaaa cgggtcatac
     1981 tctaggggct gcaggtatta tcgaattgat taattgttta gcggcaatag aggaacagac
     2041 tgtaccagca actaaaaatg agattgggat agaaggtttt ccagaaaatt ttgtctatca
     2101 tcaaaagaga gaatacccaa taagaaatgc tttaaatttt tcgtttgctt ttggtggaaa
     2161 taatagtggt gtcttattgt catctttaga ttcacctcta gaaacattac ctgctagaga
     2221 aaatcttaaa atggctatct tatcatctgt tgcttccatt tctaagaatg aatcactttc
     2281 tataacctat gaaaaagttg ctagtaattt caacgacttt gaagcattac gctttaaagg
     2341 ggctagacca cccaaaactg tcaacccagc acaatttagg aaaatggatg atttttccaa
     2401 aatggttgcc gtaacaacag ctcaagcact aatagaaagc aatattaatc taaaaaaaca
     2461 agatacttca aaagtaggaa ttgtatttac aacactttct ggaccagttg aggttgttga
     2521 aggtattgaa aagcaaatca caacagaagg atatgcacat gtttctgctt cacgattccc
     2581 gtttacagta atgaatgcag cagctggtat gctttctatc atttttaaaa taacaggtcc
     2641 tttatctgtc atttcgacaa atagtggagc gcttgatggt atacaatatg ccaaggaaat
     2701 gatgcgtaac gataatctag actatgtgat tcttgtttct gctaatcagt ggacagacat
     2761 gagttttatg tggtggcaac aattaaacta tgatagtcaa atgtttgtcg gttctgatta
     2821 ttgttcagca caagtcctct ctcgtcaagc attggataat tctcctataa tattaggtag
     2881 taaacaatta aaatatagcc ataaaacatt cacagatgtg atgactattt ttgatgctgc
     2941 gcttcaaaat ttattatcag acttaggact aaccataaaa gatatcaaag gtttcgtttg
     3001 gaatgagcgg aagaaggcag ttagttcaga ttatgatttc ttagcgaact tgtctgagta
     3061 ttataatatg ccaaaccttg cttctggtca gtttggattt tcatctaatg gtgctggtga
     3121 agaactggac tatactgtta atgaaagtat agaaaagggc tattatttag tcctatctta
     3181 ttcgatcttc ggtggtatct cttttgctat tattgaaaaa aggtgataaa atgagtaaat
     3241 ttttatttgc tcctttcatg atgaatttag gagagagcca aagattatca aaactggcta
     3301 attgtttata tgaacaagga catgatattc atatccttgg agacaattat tatccatttt
     3361 tatttaataa tgatgcttat cgctatcacc actgtgtaga agataaatct gtttataata
     3421 gtaagcgata tgatgctttc ttttctcttt caacagactt taatttttta tcagaagaag
     3481 aaattgaacg catctgttct attgaaagag acttgttacg ccaagaaaaa tttgatgctg
     3541 tattgacagg ttaccgtcta tctattgtta caagttgtcg tttagaaagc ataccattaa
     3601 tttggattat ttcaggggca acgcatatca gtgaaattgt tgaaaactca gaaggaatat
     3661 tgcctaatgg gaagataagt aaagctagta agcctcaaac aaaggatttt atcaaaagag
     3721 taatcactac ttattcaaca aatgttaaaa cgtggaataa ctatattaaa aaatatggtg
     3781 gcaaaccttt taataatgct ttagaattat ttactggtga tctaaacttg gtaacagatt
     3841 actctttgtt ttatgaattt gataaagatt cgtcctataa aacgatagga cctattttga
     3901 tagataatgt aggtttttca aaatgcagcc aaattaatca agacaataag actgtactgc
     3961 tcagctttgg tacttcattt aaacgagatt gggtggaatc ttttttaaag acattaccaa
     4021 ggcattatca ttatttactg acaagctgtg gtgaggctat aaacattcca ggaagttata
     4081 ttgagatagt tgattttatt gattttaaaa ccatagataa agagatttat ttcgctatta
     4141 tacacggtgg gcaaggaaca gtttatgcga tggctgcaca aggtattccc tttataggta
     4201 tccctttctt taacgaacaa ttttggaata ttaagaagtt cagtaaagaa aagtgcgctt
     4261 atttggtaaa gaaacctgat gtaagagaga tacgtaaagc aattagctgt cttgaaagag
     4321 actatgatat ctataaaagt tatatgataa gactaagcca aggaattaaa gaagaagcag
     4381 aggcatcttt acaaaaagcg gaggcagaaa ttgaatcctt tatcagaaaa agacaaagtt
     4441 aactatatta tgatttataa aaataggcag tctacattgg agaataattc atctggcgat
     4501 cggttgatga gatatcaaca agcaaataga caggtaagca aagataaact gttaacaggg
     4561 agttattttg tgtaccaatg cttaaaaaag ttaggtttta ccaataagat ttgtcaggaa
     4621 tctttttcta gtgttagtag ttatttaatt ggtttgccaa aggggaaaat tagttactct
     4681 aattctggtg actatcatat tctaacctat gctcccagtg gttcaactgg ggttgatatt
     4741 gagaaataca aagatagatc agaacaaacc taccaaaact acctaggaga atcagttagt
     4801 agt
//
LOCUS       DATRQA010000134         4782 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.134, whole genome shotgun sequence.
ACCESSION   DATRQA010000134 DATRQA010000000
VERSION     DATRQA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.134"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..615
                     /locus_tag="VBD27_001733"
     CDS             <1..615
                     /locus_tag="VBD27_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HEO7025722.1"
                     /translation="MGKGYTSRVFKELGKALKVKKHEHFWDIYHVKEKLSSYLRKYPI
                     ELTDFALDAVKKYNSDKLELVFDTVESLICDELEDQEFQKFKKKVLNNFKYIKPAHLR
                     NLSNRGIGIMESQHRKITYRMKRRGMYWSKWGISTMANMIILERANGLRELFFGSWRK
                     VYSEYKEGSFSAGRLFKKTDELDKFSKPLLKNGRKWSITGIKTK"
     gene            1269..4721
                     /gene="scpA/B"
                     /locus_tag="VBD27_001734"
     CDS             1269..4721
                     /gene="scpA/B"
                     /locus_tag="VBD27_001734"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEO7025723.1"
                     /translation="MRKKQKLPFDKLAIALISTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPIAVSEESRSSKETKTSQTPSDVGETVADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSHVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKENLEKAK
                     KEHGITYGEWVNDKVAYYHDYSKDGKNAVDQEHGTHVSGILSGNAPSEMKEPYRLEGA
                     MPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDETK
                     KAFDYAKSKGVSIVTSAGNDSSFGGKPRLPLADHPDYGVVGTPAAADSTLTVASYSPD
                     KQLTETATVKTDDHQDKEMPVISTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALIE
                     RGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRRDGLLLKDNP
                     PKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKL
                     SGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFS
                     PRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYY
                     QVTVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKN
                     GYFLEGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDA
                     KDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTF
                     AKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNV
                     VWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISS
                     GAKEQHTDFDVIVDNTTPEVATSATFSTEDSRLTLASKPKTSQPVYRERIAYTYMDED
                     LPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEG
                     HSNKPEQDGSDQAPDKKPEAKPEQDGSGQTPDKKKETKPEKDSSGQTPGKTPQKGQSS
                     RTLEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLAAHIFKTKRQK
                     ETKK"
BASE COUNT         1688 a          916 c          937 g         1241 t
ORIGIN      
        1 atgggtaaag gctatactag tagagttttt aaggaattag gaaaagcact taaggtaaag
       61 aaacatgagc atttttggga tatctatcat gttaaagaaa agttaagttc ataccttaga
      121 aaatatccaa ttgaattaac cgattttgct ttagatgcgg taaaaaaata taattctgat
      181 aagcttgaat tagtttttga tactgttgaa tcactgattt gtgatgaact tgaagatcaa
      241 gaatttcaga agtttaagaa aaaagtatta aataatttca aatatataaa accagctcat
      301 cttagaaatc tttcaaatcg tggtattggt atcatggaat cacaacacag aaagataacg
      361 tatagaatga agcgacgtgg catgtattgg tcaaagtggg gaatctccac aatggcaaat
      421 atgattatac ttgaaagagc taacggttta cgagaattat ttttcggttc ttggagaaag
      481 gtatacagtg agtataaaga aggttcattt agtgcagggc gactttttaa aaagacagat
      541 gaattagata aattttctaa gccccttcta aaaaatggca gaaaatggag tataacagga
      601 atcaaaacaa aatagaaaaa gtctaaagat acctttattt ctagcgtttt tttgcatgtt
      661 ttaacttgca ttaaatcttc taaagtagta taattatatt aaagaaaagc aaaaaaggtg
      721 aaggagcgcg aactcctaca ccaaaccgat taagacaaat accacttgct taatcagtcg
      781 aaccttattg tagcataact aaaacaatac cgtcaagact gaacaagcaa aaaatacttg
      841 ttttggtctt tttaagaata attcaagttt tcttaaattt atgaggtatc gactaatctc
      901 aaaatttcag acacatacct gagtgaacat caagagagaa ccgatcggtt ctctcttgat
      961 gtctaaaaga atgaggttaa ggaggtcaca aactaaacaa ctcttaaaaa gatgaccttt
     1021 actaataatc gtctttgttt tataataaag atgttagtaa tataattgat aaatgtgata
     1081 catttaatca ttatggcaaa agcaagaaaa atagctgtgt catatgcaaa taacccctgt
     1141 ttgctcttta aaaaagacgt tatccttatt tctctacaca cagacggata gctaggagag
     1201 aatcgtttga ttctctcttt tcttaatggt cataaagaca aagtctctca tcatgaaagg
     1261 acgacacatt gcgtaaaaaa caaaaactac catttgataa acttgccatt gcgcttatat
     1321 ctacgagcat cttgctcaat gcacaatcag acattaaagc aaatactgtg acagaagaca
     1381 ctcctgctac cgaacaagcc gtagaacccc cacaaccaat agcagtttct gaggaatcac
     1441 gatcatcaaa ggaaactaaa acctcacaaa ctcctagtga tgtaggagaa acagtagcag
     1501 atgacgctaa tgatctagcc cctcaagctc ctgctaaaac tgctgataca ccagcaacct
     1561 caaaagcgac tattagggat ttgaacgacc cttctcatgt caaaaccctg caggaaaaag
     1621 caggcaaggg agctgggacc gttgttgcag tgattgatgc tggttttgat aaaaatcatg
     1681 aagcgtggcg cttaacagac aaaactaaag cacgttacca atcaaaagaa aatcttgaaa
     1741 aagctaaaaa agagcacggt attacctatg gcgagtgggt caatgataag gttgcttatt
     1801 accacgacta tagtaaagat ggtaaaaacg ctgttgatca agaacacggc acacacgtgt
     1861 cagggatctt gtcaggaaat gctccatctg aaatgaaaga accttaccgc ctagaaggtg
     1921 cgatgcctga ggctcaattg cttttgatgc gtgtcgaaat tgtaaatgga ctagcagact
     1981 atgctcgtaa ctacgctcaa gctatcagag atgctgtcaa cttgggagct aaggtgatta
     2041 atatgagctt tggtaatgct gcactagctt acgccaacct tccagacgaa accaaaaaag
     2101 cctttgacta tgccaaatca aaaggtgtta gcattgtgac ctcagctggt aatgatagta
     2161 gctttggggg caagccccgt ctacctctag cagatcatcc tgattatggg gtggttggga
     2221 cacctgcagc ggcagattca acattgacag ttgcttctta cagcccagat aaacagctca
     2281 ctgaaactgc tacggtcaaa acagacgatc atcaagataa agaaatgcct gttatttcaa
     2341 caaaccgttt tgagccaaac aaggcttacg actatgctta tgctaatcgt ggtacgaaag
     2401 aggatgattt taaggatgtc gaaggtaaga ttgcccttat tgaacgtggc gatattgatt
     2461 tcaaagataa gattgcaaac gctaaaaaag ctggtgctgt aggggtcttg atctatgaca
     2521 atcaagacaa gggcttcccg attgaattgc caaatgttga ccagatgcct gcggccttta
     2581 tcagtcgaag agacggtctc ttattaaaag acaatccccc aaaaaccatt accttcaatg
     2641 cgacacctaa ggtattgcca acagcaagtg gcaccaaact aagccgcttc tcaagctggg
     2701 gtctgacagc tgacggcaat attaagccgg atattgcagc acccggccaa gatattttgt
     2761 catcagtggc taacaacaag tatgccaaac tttctggaac tagtatgtct gcaccattgg
     2821 tagcgggtat catgggactg ttgcaaaagc aatatgagac acagtatcct gatatgacac
     2881 catcagagcg tcttgattta gctaagaaag tattgatgag ctcagcaact gccctatatg
     2941 atgaagatga aaaagcttat ttttctcctc gccaacaggg agcaggagca gtcgatgcta
     3001 aaaaagcttc agcagcaacg atgtatgtaa cagataagga caatacctca agcaaggttc
     3061 acctgaacaa tgtttctgat aaatttgaag taacagtaac agttcacaac aaatctgata
     3121 aacctcaaga gttgtattac caagtaactg ttcaaacaga taaagtagat ggaaaacact
     3181 ttgccttggc tcctaaagca ttgtatgaga catcatggca aaaaatcaca attccagcca
     3241 atagcagcaa acaagtcacc gttccaatcg atgctagtcg atttagcaag gacttgcttg
     3301 cccaaatgaa aaatggctat ttcttagaag gttttgttcg tttcaaacaa gatcctacaa
     3361 aagaagagct tatgagcatt ccatatattg gtttccgagg tgattttggc aatctgtcag
     3421 ccttagaaaa accaatctat gatagcaaag acggtagcag ctactatcat gaagcaaata
     3481 gtgatgccaa agaccaatta gatggtgatg gattacagtt ttacgctctg aaaaataact
     3541 ttacagcact taccacagag tctaacccat ggacgattat taaagctgtc aaagaagggg
     3601 ttgaaaacat agaggatatc gaatcttcag agatcacaga aaccattttt gcaggtactt
     3661 ttgcaaaaca agacgatgat agccactact atatccaccg tcacgctaat ggcaaaccat
     3721 atgctgcgat ctctccaaat ggggacggta acagagatta tgtccaattc caaggtactt
     3781 tcttgcgtaa tgctaaaaac cttgtggctg aagtcttgga caaagaagga aatgttgttt
     3841 ggacaagtga ggtaaccgag caagttgtta aaaactacaa caatgacttg gcaagcacac
     3901 ttggttcaac ccgttttgaa aaaacgcgtt gggacggtaa agataaagac ggcaaagttg
     3961 ttgctaacgg aacctacacc tatcgtgttc gctacacgcc gattagctca ggtgcaaaag
     4021 aacaacacac tgattttgat gtgattgtag acaatacgac acctgaagtc gcaacatcgg
     4081 caacattctc aacagaagat agtcgtttga cacttgcatc taaaccaaaa accagccaac
     4141 cggtttaccg tgagcgtatt gcttacactt atatggatga ggatctgcca acaacagagt
     4201 atatttctcc aaatgaagat ggtaccttta ctcttcctga agaggctgaa acaatggaag
     4261 gcgctactgt tccattgaaa atgtcagact ttacttatgt tgttgaagat atggctggta
     4321 acatcactta tacaccagtg actaagctat tggagggcca ctctaataag ccagaacaag
     4381 acggttcaga tcaagcacca gacaaaaaac cagaagctaa accagaacaa gacggttcag
     4441 gtcaaacacc agataaaaaa aaagaaacta aaccagaaaa agatagttca ggtcaaacac
     4501 caggtaaaac tcctcaaaaa ggtcaatctt ctcgtactct agagaaacga tcttctaagc
     4561 gtgctttagc tacaaaagca tcaacaagag atcagttacc aacgactaat gacaaggata
     4621 caaatcgttt acatctcctt aagttagtta tgaccacttt cttcttggga ttagcagctc
     4681 atatatttaa aacaaaacgc caaaaagaaa ctaaaaaata gtattactta aactgataag
     4741 agcttattta atagagagaa ccaatttggt tctctctatt tt
//
LOCUS       DATRQA010000135         4781 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.135, whole genome shotgun sequence.
ACCESSION   DATRQA010000135 DATRQA010000000
VERSION     DATRQA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.135"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            29..901
                     /locus_tag="VBD27_001735"
     CDS             29..901
                     /locus_tag="VBD27_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7025724.1"
                     /translation="MSKKIKNFILLNIGSLIVAFGFNAFFLHNHIVTGGISGLAVSFH
                     EVFGWDSAFFIMIINIPLLLLCYFGLGKQTFLKTVYGSWIFPVFIKLTQSVPTLTHNP
                     LLAALFGGVIVGCGLGIVFWSDSSTGGTGIIIQFLGKYTPISLGQGVILIDGLVTIVG
                     FLAFDSDTVMFSIIGLITISYIINAIQTGFTTLSTVLIVSQEHQKIKTYINTVADRGV
                     TEIPVKGGYSGTNQIMLMTTIAGYEFAKLQEAIAEIDETAFITVTPTSQASGRGFSLQ
                     KNHGRLDEDILMPM"
     gene            967..2586
                     /locus_tag="VBD27_001736"
     CDS             967..2586
                     /locus_tag="VBD27_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7025725.1"
                     /translation="MLTVSDVSLRFSDRKLFDEVNINFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHIALGPDERLSVLRQNHFDYEDERVIDVVIMGNETLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEELHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKESSELAARLQADRNAKAEEKIKQLQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFVNFKAEREMGNDLLTVENLSVTIDGEKI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSRSYLPKDNSR
                     DFASGESILEWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKDFKESIIFASHDHEFIQTLANHIIIL
                     SKNGVIDRIDETYDEFLENTEVQAKVAQLWK"
     gene            2709..>4781
                     /locus_tag="VBD27_001737"
     CDS             2709..>4781
                     /locus_tag="VBD27_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO7025726.1"
                     /translation="MTINHYKLRIPYYTISFLLPFIIIVCILFTKNIYWGSPTTILAS
                     DSFHQYVIFNQALRNILHGSNSLFYTFTSGLGLNFYALSSYYLGSFLSPIVYFFNLKN
                     MPDAIYLLTICKVGLIGLSMFVTLCKRHCKVNRVLLLVISTCYSLMSFSISQIEINMW
                     LDVFILIPLVVLGVDQLLWERKPILHFLSLTALFIQNYYFGFMTTIFTSLYFIVQITR
                     NTDSKVAFKQFLHFTFLSLLAGMTSSIMILPTYFDLTTHGEKLTKVSKMFTENSWYMD
                     LFAKNMIGAYDTTKFGSIPMIYVGLLPLLLSLLYFTIKEIPRRTRLAYGFSLIFVIAS
                     FYITPLDLFWQGMHAPNMFLHRYSWVLSVLICLLAAECLEYLDNISWKKILGVNLILI
                     SGFIITFLFKKHYHYLNLELLLLTLTFLSAYIILTISFVSKQIPKLVFYPFLIGFVVL
                     EMTLNTFYQLNSLNDEWVFPSRQGYAKYNHSISKLVRKTERNNSTFFRTERWLGQTGN
                     DSMKYNYNGISQFSSIRNRSSSQVLDRLGFKSDGTNLNLRYQNNTLIADSLFGVKYNL
                     TEYPFDKFGFIRKAQDKQTILYENQFASQLAILTNQVYQDKPFTVNTLDNQTTLLNQL
                     SGLKETYFEHLIPNSVSGQTTLNKQVFVRKNKQGNTEITYNITIPKNSQLYVSMPFIN
                     FNNEENKIV"
BASE COUNT         1525 a          788 c          801 g         1667 t
ORIGIN      
        1 tattattact aaagttaagg agcttatcat gtctaaaaaa attaagaatt ttatactatt
       61 aaatattgga tctctaattg ttgcatttgg tttcaacgct ttctttcttc ataatcatat
      121 tgtcacgggg gggattagtg gtttggcagt tagttttcat gaagtatttg gttgggattc
      181 tgcttttttt attatgatta tcaatattcc attgctcctt ctttgctact ttggcttagg
      241 taaacaaacc tttttaaaaa ctgtctatgg ttcttggatt tttcctgttt ttattaagtt
      301 aacacaaagt gtaccaactt tgacccacaa cccactcctc gcagcacttt ttggaggtgt
      361 tattgtagga tgtggtttgg ggattgtttt ttggagcgac tcttcaactg gtggaacggg
      421 gattatcatt caattcttag gaaaatatac tcctataagc cttggacaag gggttatatt
      481 gattgatgga cttgttacaa ttgttggttt cctagctttt gacagtgata cggttatgtt
      541 ttctattatt gggttgataa ctattagtta tattattaat gctatccaaa ctggatttac
      601 aaccttaagc actgtcttaa tcgtttctca agagcaccaa aaaattaaga catatatcaa
      661 tactgtcgca gatagaggag taacagaaat tcccgttaaa gggggatatt ctggaactaa
      721 tcaaatcatg cttatgacaa ctattgctgg ttatgagttt gctaaattac aagaggcaat
      781 agcagaaatt gacgaaacag ccttcataac agtaactcca acatcacaag cttctggacg
      841 tggatttagt cttcaaaaaa atcatggacg tcttgatgaa gacattctta tgccaatgta
      901 atcgattgat aattaaagct ttttctagta gaatagcaga tagaatatac acaagaggta
      961 acaaaattgc ttacagtttc agatgtttca ctacgtttta gtgatcgcaa attatttgat
     1021 gaagttaaca ttaactttac agcaggaaat acctatggtc ttatcggagc aaatggcgct
     1081 ggtaaatcaa ctttccttaa aattttagct ggtgatattg aaccaacaac gggacatatt
     1141 gcacttggcc cagatgaacg cctttctgta cttcgccaaa atcattttga ttatgaagat
     1201 gagcgcgtca ttgatgtcgt tatcatgggt aatgaaacac tttatagtat catgaaagaa
     1261 aaggatgcta tctacatgaa agaagatttt tctgatgaag atggtgttcg cgctgcggaa
     1321 ttagaaggag aatttgctga actcggtgga tgggaggctg aaagtgaggc ttctcaatta
     1381 ctccaaaatc taaatatttc agaagagctt cattatcaaa atatgagtga attagctaat
     1441 ggagacaaag ttaaagttct ccttgcaaaa gctctctttg gtaaaccaga tgtacttctc
     1501 cttgacgagc ctaccaatgg acttgatatc cagtctatta cttggttaga agatttcttg
     1561 attgactttg aaaatacagt tattgtagtt tcccacgacc gtcatttctt gaataaagta
     1621 tgtacacata tggctgacct tgattttgga aaaattaaac tgttcgtcgg caactatgat
     1681 ttctggaaag aatcatcaga attagcagct cgtttacaag ctgatcgtaa tgctaaagca
     1741 gaagaaaaaa ttaaacaact tcaagaattc gttgcacgat tttcagcgaa tgcttcaaaa
     1801 tcaaaacaag caacttctcg taaaaaaatg cttgataaaa ttgaacttga agagattgta
     1861 ccatcaagcc gtaaataccc attcgttaac tttaaagccg aacgcgaaat gggaaatgat
     1921 ttattgaccg ttgaaaatct ctctgtcact attgatgggg aaaaaatcct tgataatatc
     1981 agtttcattc ttcgtcctgg tgataaaact gcacttattg gacaaaatga tatccaaact
     2041 actgcactta ttcgagcatt gatgggggat atcgaatatg aaggaacaat caaatgggga
     2101 gtaacaacta gtcgttctta ccttccaaaa gacaattcac gagactttgc ttctggcgag
     2161 tcaatcttag aatggcttcg tcaatttgct agtaaagaag aagacgataa taccttctta
     2221 cgtggcttcc taggtcgtat gcttttctct ggagatgagg ttaacaaatc tgttaatgtc
     2281 ttatctgggg gtgaaaaagt acgcgttatg ctttcgaaat taatgctatt aaaatctaat
     2341 gttcttgttc ttgatgatcc aacaaaccac ttagatttgg aatctatttc aagtctcaat
     2401 gatggactaa aagatttcaa agaatctatt atttttgcaa gtcatgacca cgagtttatt
     2461 caaactttag cgaaccatat tattattctt tctaaaaacg gtgttatcga tcgtatcgat
     2521 gaaacttacg atgaatttct tgaaaatacc gaagtacaag ctaaggtcgc tcaactctgg
     2581 aaataatcct ttggcatatc tctaacctaa taaacgtgtc aagattgaaa atcttaaaac
     2641 tatattaatc atttaaataa aaaggttgga acaaatagcg tccagccttt ttatcttagg
     2701 agatatatat gacaattaac cactataagc ttcgtatacc ttactataca ataagctttc
     2761 tacttccctt cataatcata gtctgtatcc tctttactaa gaatatttat tggggaagtc
     2821 caacaaccat attagcaagc gatagctttc atcaatatgt tatctttaat caagcattaa
     2881 gaaatattct tcatggttca aacagcttat tctatacctt tacaagcggt ttaggtttaa
     2941 atttttatgc tctgagtagt tactatttgg gaagtttttt atccccaata gtttatttct
     3001 ttaacctaaa aaatatgcct gatgctatct accttttaac aatatgtaaa gtagggttga
     3061 taggtttgtc aatgtttgtc actctttgta aacgtcattg taaagttaat cgtgtcttac
     3121 ttttagtaat ttctacttgc tatagcttaa tgagtttctc aattagccaa attgaaatta
     3181 atatgtggct agatgtattt attctaattc cccttgttgt tttaggagtt gatcagctac
     3241 tatgggaaag aaaacctata cttcattttc tctctctgac agccttattc atccaaaact
     3301 attattttgg ttttatgact accatcttta ctagccttta cttcatcgta caaataaccc
     3361 gtaatactga tagtaaggtt gcatttaaac aatttcttca ttttaccttt ttatctcttt
     3421 tagctggtat gactagtagc attatgattt tgccaactta ttttgattta actactcatg
     3481 gtgaaaagtt gacaaaggta tcgaagatgt ttacagaaaa ttcttggtac atggatttgt
     3541 ttgctaaaaa tatgattgga gcctatgata caacaaaatt tggctctatt cctatgattt
     3601 atgtaggttt acttccttta ctcctttctt tactttactt tacaataaag gaaattcctc
     3661 gaaggactag attagcatat ggattttcac taatatttgt tattgctagt ttttatataa
     3721 cacctctaga tttattttgg caagggatgc atgctccaaa tatgtttctt catcgctact
     3781 cttgggtact ttcagtactt atctgtctac tagctgctga atgccttgag tatttagaca
     3841 acatttcttg gaaaaagatt ttaggggtca atttaatttt aataagtggt ttcataatca
     3901 cgtttttatt taaaaagcac tatcattacc ttaatcttga attacttctt ttaaccttaa
     3961 cattcctctc agcttatatt attctaacta ttagttttgt cagtaagcaa atccctaaat
     4021 tagtttttta tccctttctt attggttttg ttgtgttaga gatgacatta aacacctttt
     4081 accaactaaa tagtcttaac gatgagtggg tatttccatc taggcaagga tatgctaaat
     4141 ataatcacag cattagcaaa cttgtcagaa agactgagag aaataactct acattcttcc
     4201 gtacagagcg ctggttagga caaaccggca acgactctat gaaatataat tataatggta
     4261 tttcacaatt ttcatctatt agaaatcgtt cttcaagtca agtgcttgat agacttggct
     4321 ttaaatcaga tggtacaaat ttaaatttac gttatcaaaa taatactttg atcgcagata
     4381 gtctatttgg tgtaaagtac aaccttacag aatatccttt tgataaattt ggttttatca
     4441 gaaaagctca agataaacaa accattcttt acgaaaatca gtttgccagt caattagcta
     4501 tactaacgaa tcaagtatat caagataaac catttactgt gaatactttg gataatcaga
     4561 caactctttt aaatcaatta agtggtttga aagagacgta ttttgagcat cttattccaa
     4621 atagcgtttc tgggcaaact actcttaata aacaagtatt tgttagaaag aataaacaag
     4681 ggaatactga aattacctat aacataacta tccctaaaaa cagtcagtta tatgttagta
     4741 tgccgtttat aaattttaat aatgaggaaa ataaaattgt t
//
LOCUS       DATRQA010000136         4746 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.136, whole genome shotgun sequence.
ACCESSION   DATRQA010000136 DATRQA010000000
VERSION     DATRQA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.136"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(172..1407)
                     /locus_tag="VBD27_001738"
     CDS             complement(172..1407)
                     /locus_tag="VBD27_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO7025727.1"
                     /translation="MATSTENLQLAKRGPIISIIAYITLAVAKLAAGYWFDATSLVAD
                     GFNNLSDILGNVALLIGLHLASQPADSNHRFGHWKIEDLASLITSFIMFVVGIQVFIQ
                     TVTKIINNTDTNIDPLGAIVGAISALVMLGVCFYNKQLSQRVKSSALVAASKDNLSDA
                     VTSIGTSIAIIAASLNFPIIDRLAAIIITYFILKTAYDIFIESAFSLSDGFDDYQLKQ
                     YEKAILTIPKISAVKSQRGRTYGSNIYLDIVLEMNPDLSVFESHAITEKVEKLLSDKF
                     SVYDIDIHVEPASIPEDEIFDNVYQKLYKNEKIILAKIPGYETFISPDFYMINEKGNI
                     ITSDMLTNATNHSLASNFKYFNVKSISQKTKLVSYELEGKRHTSIWRRNEKWFLIYHQ
                     ITAKSSPYKTRRYQITSLD"
     gene            complement(1471..2817)
                     /locus_tag="VBD27_001739"
     CDS             complement(1471..2817)
                     /locus_tag="VBD27_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEO7025728.1"
                     /translation="MLTLFTHINSFVWGPPLLALLVGTGIYLSFRLGFVQLRQLSRAF
                     KLIFREDNGQGDISSYAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGLLAIKYRTKDTNGEISGGPMYYIVNGMGQKWKPLAVFFSAAGILVALLGIG
                     TFTQVNAIASSLEHTFKISTRFTSLILAVIVLFIIFGGIKSISKVSEKIVPFMAISYI
                     LATLIIIAVNYNKIPHTFQLIFSGAFSGTAAIGGFSGAIVKEAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTKEPVEQGLISMTGTFIDTIVICTLTGIAILVTGKWLEFDLQGAPL
                     TQASFNTVFGSLGSFALTFCLVLFAFTTILGWSYYGERCFEYLLGTKFINAYRIIFVI
                     MVGLGGFLQLDLIWVIADIVNGLMALPNLIALLALSPIIVKETQKYFSETKNITKP"
     regulatory      complement(2974..3106)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3414..4739
                     /locus_tag="VBD27_001740"
     CDS             3414..4739
                     /locus_tag="VBD27_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEO7025729.1"
                     /translation="MTLNAKGESNQILVDKEVHSTNLLLDIDEKPELFQGLLLSFQHV
                     FAMFGATILVPLILGMPVSVALFASGCGTLIYQVATKFKVPVYLGSSFAYITAMALAM
                     KQMHGDISAAQTGILFVGLIYVVVATVIKFVGNSWVDKILPPIIIGPMIIVIGLGLAN
                     SAVTNAGFVAKGDWRKMLVAVVTFLIAAFINTKGKGFIKIIPFLFAIIGGYILSIILG
                     LVDLSPVEKAAWFELPKFYLPFKTGLFHSYKLYFGPEMLAILPISIVTIAENIGDHTV
                     LGQICGRNFLKKPGLNRLLIGDGLATAFSALIGGPAETTYGENTGVIGMTRIASVTVI
                     RNAAFIAIAFSFFGKFTALISTIPSAVLGGMAILLYGVIASNGLKVLIENRVNFAEVR
                     NLIIASSMLVLGLGGAVLDLGALTLSGTALSAIVGIILNLILPKESMKS"
BASE COUNT         1526 a          797 c          858 g         1565 t
ORIGIN      
        1 cgtataattt catgaaaaac tccttccatt atagtctact actctgaaag taattttcaa
       61 ggctatgaaa cgtttacata aaaacaaatt aaaaagacgg agattcttct caatcttttt
      121 aatttgtttt tgtaagctat ttttatcaag agtaactgat taaatagtat attaatcaag
      181 ggaagtgatt tgatatcgtc tcgttttata cggacttgac ttggcagtta tttgatggta
      241 gattaagaac catttttcat tacggcgcca aatactagta tgtcgtttac cttccaattc
      301 ataagacaca agttttgttt tttgactaat tgattttaca ttgaaatatt taaagttgct
      361 tgccaatgaa tgatttgtag cattagtaag catgtcagaa gtgataatat ttcccttttc
      421 attaatcata taaaagtcag ggctaataaa agtctcataa ccggggattt ttgctaggat
      481 gattttttcg tttttgtata atttttggta aacattatcg aaaatttcgt cttctgggat
      541 agaagcgggt tctacatgaa tatctatgtc atatacagaa aatttatcac tgagtagttt
      601 ttccactttt tcggtaatgg catgactttc aaagactgag agatcagggt tcatttcgag
      661 aacaatatca aggtaaatat tactaccgta agtacgtcca cgttgagatt ttacagcgga
      721 tattttagga attgttaata tagctttttc gtattgtttt aattgataat catcgaaacc
      781 atccgataag ctgaaagcag attctataaa aatatcataa gctgttttaa gaataaaata
      841 agtaataatg atagcagcta aacgatcaat aataggaaaa tttagagaag ccgcaatgat
      901 agcaatagat gtgccgatag atgtcacagc atcagaaagg ttatctttag aagctgcaac
      961 cagagcgcta gactttactc tttgagataa ttgtttatta tagaaacaaa cgccaagcat
     1021 aacaagagca gatatagcac ctactatagc acctagagga tcaatgttag tatcagtatt
     1081 attaataatc ttagtgacag tttgaataaa aacttgaata cctacgacaa acatgataaa
     1141 agaggtaatc aggcttgcta aatcttcaat tttccagtga ccaaaacgat ggtttgagtc
     1201 ggctggttga ctagccaagt gtaaaccaat taagagtgct acatttccta agatatcaga
     1261 taagttatta aatccatctg ctacaagtga agtagcatca aaccaataac ctgcagctaa
     1321 tttagcgact gccagtgtta tataggcaat gatactaata ataggccctc tttttgcaag
     1381 ttgtaagttt tcagtagaag ttgccatttt cctaatttcc tttcatctac acattatatc
     1441 acttaggagc agactataaa actagtaagt tcaaggctta gtgatatttt tagtctctga
     1501 aaaatatttc tgtgtttctt taacaataat tggtgacaaa gctaggagag ctattagatt
     1561 tggtaaagcc attaaaccat taacgatatc agcgataacc caaatgagat cgagctgtaa
     1621 gaaaccacct aagccgacca taatgacaaa aataatgcga taagcgttaa taaatttagt
     1681 ccccaataga tattcaaagc aacgctctcc gtagtagctc catcctaaaa tagtagtaaa
     1741 agcaaataag acaaggcaga aggttagggc aaaactacct agtgagccaa atacggtatt
     1801 aaaagatgct tgtgttaacg gagcaccctg taaatcaaat tctagccatt ttcctgtaac
     1861 taaaattgca atccctgtca aagtacaaat aacaatagtg tcaatgaagg ttcctgtcat
     1921 tgaaataagc ccttgttcaa caggttcttt tgttttagct gctgctgcag ctattggagc
     1981 agaacccaat ccagattcat ttgagaaaac tcctctagca attccctttt gaattgcttc
     2041 tttaacgata gctccagaaa aaccaccaat ggctgctgtc ccagaaaaag caccgctaaa
     2101 gatgagttgg aaagtatgtg gaatcttatt gtaattaact gcaataataa tcagagttgc
     2161 taaaatatag cttatagcca taaaaggcac tattttttct gaaacttttg aaatagattt
     2221 tatacctcca aatataataa ataatactat aacagcaaga atgaggctgg taaatctagt
     2281 tgatattttg aaagtatgct caagagaaga ggcaattgca ttgacttgag tgaatgtccc
     2341 tattcctaaa agagctacta agatacctgc tgcagaaaaa aagactgcta atggtttcca
     2401 tttttgtccc attccattga caatgtagta cattggtccc ccagaaattt ctccattagt
     2461 atcttttgtg cgatatttga tagctaataa tccttctgcg tattttgttg ccattccaaa
     2521 aaaggcggct acccacatcc aaaacaaagc tcctggtcct ccagatttaa tagccgtagc
     2581 cacaccaacg atattacctg tccctaccgt tgcagcaaga gcagttgcaa gagcagcata
     2641 acttgaaata tctccttgtc cattatcttc tcggaaaatc aatttgaaag ctctagaaag
     2701 ttgtctcaat tgaacaaaac ctaagcgaaa tgataggtaa atacctgttc cgactaataa
     2761 agcaagtaaa ggtggacccc aaacgaagct attgatatga gtaaaaagtg ttagcatatg
     2821 ttctcctttg atacatgaca tacctatgga ttctgtaatg aaacgaaaga agttatctct
     2881 aaaagagata acttctgaaa gatgctagct aaaaagcgca caaataaagg tagtagtaaa
     2941 cttaataagc ttactactca ttattaatat tggcttttag cttctgtcct tttgcctgag
     3001 agattggagg agttgtttta ctcttcttgc cccttcggcg cctagagcac atcttggtgt
     3061 caatacaaag atgttgctac ttataggtct ctccagaagt ccgtctgtca atcagtcctg
     3121 tcttaagaca cctgagagtg taattccttc ggcgaaagtc taactttctt ctcctgaatg
     3181 acgtcattcg gtatatttta ttatgttaac tatcataatg aaaaagcata gttttgtcaa
     3241 tatagtttca aaaaactctt gttttctaaa atgtcttatg ctataattct ttcagcgtag
     3301 gtttttaaac tcatcccgtg aggtgagaaa aacatcagga aaaaaataaa gcctatgact
     3361 atattgctat agaaagtggg cttttttgta tgcccaagag gactagttta gatatgactt
     3421 taaatgctaa aggtgaaagt aatcaaattc tagtagataa ggaagtacat tctactaact
     3481 tattactaga tatcgatgaa aaaccagaat tatttcaagg tttgctttta agttttcaac
     3541 atgtttttgc catgtttgga gctacgatat tagttccgct tattttaggg atgcctgtat
     3601 cagtggcttt atttgcttct ggttgcggaa cgctcattta tcaagttgcg acaaaattta
     3661 aagtccctgt ttatctaggc tcatcatttg cttatattac agctatggca ttagccatga
     3721 aacaaatgca tggcgatatt tcggctgctc aaacaggtat tctttttgta ggtctaatct
     3781 atgtcgttgt ggcgacggtt attaagttcg taggaaattc atgggttgat aagattttgc
     3841 caccaataat cattggacct atgattattg ttattggttt aggtttggca aactctgctg
     3901 taactaatgc aggttttgtc gctaaaggtg attggcgtaa aatgctagtt gctgttgtta
     3961 cattcttgat tgcagctttt atcaatacta aagggaaagg ttttatcaag attattccat
     4021 ttttatttgc aattattgga ggttatattt tatcaatcat tcttggcttg gtagatttat
     4081 ctccagttga aaaagctgcc tggtttgaat taccaaaatt ctatttaccg tttaagacag
     4141 gtttatttca cagttataaa ctttactttg gaccagaaat gcttgcaatt cttccaatct
     4201 caattgtaac gattgctgaa aatatcggtg atcatacagt tttaggtcag atttgtgggc
     4261 gtaactttct aaaaaaacca ggtttaaatc gtcttttaat tggagatggt ttagcaaccg
     4321 cattttctgc tcttatagga gggccagctg aaactactta tggtgaaaat acaggtgtta
     4381 ttggcatgac gcgtatagct tctgtaacgg ttattcgtaa tgcagctttt attgcaatcg
     4441 ctttttcatt ctttggtaaa tttacagctt tgatctctac aattcctagt gcagtacttg
     4501 gtggaatggc tatattactt tatggtgtta ttgcaagtaa tggattaaaa gtgttgattg
     4561 aaaatcgtgt caattttgct gaggttcgta atctgattat tgccagttcc atgttagttt
     4621 taggcctcgg tggagcagtc cttgatctag gtgctctaac tttatcagga acagcattaa
     4681 gtgctatagt gggtattata ttaaatttaa tattgccaaa agaaagcatg aaatcataac
     4741 aaaaga
//
LOCUS       DATRQA010000137         4672 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.137, whole genome shotgun sequence.
ACCESSION   DATRQA010000137 DATRQA010000000
VERSION     DATRQA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.137"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..240
                     /locus_tag="VBD27_001741"
     CDS             <1..240
                     /locus_tag="VBD27_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO7025730.1"
                     /translation="LDIHYVIEGYEVIKLGKGDKVEVEEYLGDIGFVKCSEETSFDLR
                     DNYIAFFFPEEAHQPNGMGSLGNYVKKGVLKVLMA"
     gene            260..922
                     /locus_tag="VBD27_001742"
     CDS             260..922
                     /locus_tag="VBD27_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEO7025731.1"
                     /translation="MKKANQLIAAIFDLNNPFMQGCNVVFDLALLNLLFMITCLPLVT
                     IGAAKISLYRTLWQKLEGDQTNLLILYIKHLKKEWFQGMLLGLVELSILVVIIFDLTI
                     LHYQIGFIVPFLKIICYAFLLLTVMTSIYLFPMAARYEMSLLDTVKKSFIMACLNLKW
                     TGVLMFLLIMTWFIMVQSSLLFMLTVSAIFIFAYTAFAYFKIIILQKQFAYFSKQQGD
                     YQ"
     gene            919..1836
                     /locus_tag="VBD27_001743"
     CDS             919..1836
                     /locus_tag="VBD27_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEO7025732.1"
                     /translation="MKDLQKYQGIIPAFYACYDDKGDICPERVKALTNYFIDKGVQGL
                     YVNGSSGECIYQSVADRKLVLENVMSVAKGKLTVIAHVACNNTKDSVELAMHAEAMGV
                     DAIAAIPPIYFRLPEYAIADYWNTISQAAPQTDFIIYNIPQLAGVALTSDLYRKMLQN
                     PQVIGVKNSSMPVQDIQNFVAIGGENHIVFNGPDEQFLGGRLMGAAAGIGGTYGVMPE
                     LYLTLNQLIVDKDLEKARELQFTINDIITKLCSGHGNMYAVIKAVLEINEQLTIGSVR
                     LPLASVTEEDKPIIKEAAEMIRHAKKQFC"
     gene            1853..2734
                     /locus_tag="VBD27_001744"
     CDS             1853..2734
                     /locus_tag="VBD27_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO7025733.1"
                     /translation="MTRTVAIDIGGTMIKHGIVDNLGCIVEASELATEAYKGGPGILQ
                     KVCQIIDNYLTEGSIDGIAISSAGMVDPDEGCIFYSGPQIPNYAGTQFKKVLEDTYQV
                     RTEIENDVNCAGLAEAVSGSAKDSSIALCLTIGTGIGGCLIIDKTVFHGFSNSACEVG
                     YMHLSDGDFQDLASTTALIADVAKAHGDEINRWDGRRIFQEAKEGNEKCIASIDRMIN
                     YLGQGIANMVYVVNPEKVVLGGGIMAQKDYLQDKLSESLKRNLVTSLAEKTIIVFAQH
                     ENQAGMLGAYYHFKNRG"
     gene            2742..3719
                     /locus_tag="VBD27_001745"
     CDS             2742..3719
                     /locus_tag="VBD27_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO7025734.1"
                     /translation="MIETMSLDDMREYLGQDQIPEDFDDFWKKQTMKYQGNIEYRLDK
                     KDFNITFAQAYDLHFKGSNNSIVYAKCLFPKTNKPYPVVFYFHGYQNQSPDWSDQLNY
                     VAAGYGVVSMDVRGQAGQSQDKGHFDGITVKGQIVRGMISGPNHLFYKDIYLDVFQLI
                     DIIATLESVDSNQLYSYGWSQGGALALIAAALNPKIVKTVAVYPFLSDFRRVLDLGNV
                     SEPYDELFRYFKYSDPFHKTENNVLKTLAYIDVKNFAHRISCPVVLLTALKDDICPPS
                     SQFAIFNRLTSTKKHLLLPDYGHDPMTVQVKDYIFDQLTGSQFTKQKIE"
     gene            complement(3742..4545)
                     /locus_tag="VBD27_001746"
     CDS             complement(3742..4545)
                     /locus_tag="VBD27_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO7025735.1"
                     /translation="MTIYDQIESALDLMTDLEHEIACYFMGQPISKDALASSIVTKQL
                     HISQAALTRFAKKCGFKGYREFVFEYLKSHETISQQLYGLQNDNTKKVFMNYQEMVSK
                     SADIIDEEQLLEVSHMIEQADRVYFYGKGSSSLVAKEFKIRLMRLGVICEALDDTDSF
                     SWTNSIVNDRCLVIAFSLSGNTNSVIGALKIASCHGAKTVLFTKQPHTIDYAFDKIIQ
                     VASARHLDYGNRISPQIPMLIMVDIIYAQFLDINKIEKERIFRETIIQR"
BASE COUNT         1501 a          732 c          923 g         1516 t
ORIGIN      
        1 cttgatatac attatgtcat tgaaggatat gaagttatca aattgggaaa aggtgataag
       61 gtagaggtag aggaatactt aggcgatata ggatttgtca agtgctcaga agagacttct
      121 tttgatttga gagataatta tatagccttc tttttcccag aagaagctca tcagccaaat
      181 ggtatgggaa gtctaggtaa ttatgtcaaa aaaggggtat tgaaagtgtt aatggcgtaa
      241 gaaatacagg gagtatattg tgaaaaaagc aaatcaatta atagcagcga tttttgatct
      301 taacaacccc tttatgcaag gttgcaatgt tgtttttgat ctggcattgc taaaccttct
      361 ctttatgata acttgcctac cattggtaac tattggtgct gctaaaatta gtttgtatcg
      421 aacgttgtgg caaaaattag aaggtgatca gacaaatctt ttaatattgt atataaaaca
      481 cctaaaaaaa gagtggttcc aaggtatgct tttaggtttg gttgaactaa gtattttggt
      541 tgtgatcata tttgatttaa caattcttca ttatcaaata ggtttcattg tgcctttctt
      601 gaagataatt tgttacgcct ttttgttatt gactgtgatg acgagcatat acctatttcc
      661 tatggctgcc cgctatgaaa tgtctctttt agataccgtg aaaaagagct ttataatggc
      721 atgtttaaac ttgaaatgga ctggtgtatt gatgttttta ttaatcatga cttggtttat
      781 tatggtgcag tctagtctct tatttatgct tacagtgtca gctatattta tttttgctta
      841 taccgccttt gcttacttta aaattattat tttacaaaaa caatttgcct atttcagcaa
      901 acaacaaggg gattatcaat gaaagattta caaaaatatc aaggaattat tcctgcattt
      961 tatgcttgct atgatgataa gggtgatatt tgtcccgaac gagtaaaagc cctgaccaat
     1021 tattttattg ataaaggtgt tcaaggactt tatgttaatg gttcatcagg agaatgtatt
     1081 taccaaagtg tagcagatcg taaacttgta ctggaaaatg ttatgtccgt tgctaagggt
     1141 aaactaacag tgatagcgca tgtagcatgt aataatacta aggatagcgt cgagttggct
     1201 atgcatgctg aagctatggg tgttgatgct attgcagcaa ttccaccaat ttattttcga
     1261 ttaccagagt atgccatagc agattattgg aatactatta gtcaagcagc accacaaact
     1321 gattttatta tttacaatat tcctcaattg gcaggggttg ctttgacatc ggatttatat
     1381 cgaaaaatgt tacaaaatcc gcaagtaatt ggtgttaaaa attcttccat gccagttcaa
     1441 gacattcaga attttgtggc tattggagga gaaaatcata ttgtttttaa tggtccagat
     1501 gaacaatttt tgggtggtcg tctcatggga gcagctgcag gtattggtgg tacctatggc
     1561 gtaatgccag aattatactt gactttaaat cagttaattg ttgataaaga tttagaaaaa
     1621 gcgcgtgaac ttcaatttac aattaacgat attatcacaa agctttgttc gggtcatggt
     1681 aatatgtatg ctgtgatcaa agctgtattg gaaatcaatg agcaactaac tataggttcc
     1741 gttcggttac ctttagcatc tgtaacagaa gaagataaac ctattattaa agaggcagca
     1801 gaaatgatta gacatgctaa aaaacagttt tgttaaagtg aggtagataa ttatgaccag
     1861 aacagtagca attgatattg gcggtacaat gattaagcat ggcattgtgg ataatttagg
     1921 ttgtattgtt gaggcaagtg agcttgccac agaagcctac aaaggtggtc caggcatttt
     1981 acaaaaagtt tgtcaaatta tcgataacta tctaacagaa gggagcattg atggaattgc
     2041 tatctcatct gctggcatgg tagatccaga tgaaggatgc atattttact ctggtcctca
     2101 aattccaaac tatgctggga cacaatttaa gaaagtttta gaagacactt atcaagtacg
     2161 cacagaaatt gaaaatgatg ttaattgtgc aggattagcg gaagctgtct caggtagtgc
     2221 caaagatagt tcaatagcgc tttgtcttac tattggaaca ggtattggtg gttgtctgat
     2281 tattgataaa acagtttttc atggctttag taattctgct tgtgaagttg gctatatgca
     2341 cctttcagat ggtgattttc aagatttagc ctctacaact gcactgattg ctgatgttgc
     2401 caaagcgcat ggagatgaaa ttaaccgatg ggatggacgt cgtatttttc aggaggctaa
     2461 agaaggcaat gagaaatgta ttgcaagtat agaccgtatg ataaactacc ttggtcaagg
     2521 aatcgctaat atggtttatg tggtaaatcc tgaaaaagtt gttttaggtg gtggaatcat
     2581 ggctcaaaaa gattatttac aagataaact tagtgaatct ttgaaacgaa atcttgtgac
     2641 aagtcttgct gaaaaaacaa taatagtttt tgcacaacac gaaaaccaag ctggtatgct
     2701 aggagcttac taccatttta aaaacagggg gtgacgggta tatgattgaa acaatgtcgc
     2761 tagatgacat gagagaatac ctaggtcaag accaaattcc cgaggatttt gatgacttct
     2821 ggaaaaaaca aacaatgaaa tatcaaggga atattgagta tcgtctagac aaaaaagatt
     2881 tcaatattac ttttgcacaa gcttatgatc ttcattttaa aggcagcaat aactctattg
     2941 tctatgccaa atgtttattc cctaaaacaa ataaacccta tcctgtagtt ttttattttc
     3001 atggttacca aaaccaaagt cctgactgga gtgaccaact taattatgta gcagcaggtt
     3061 acggcgttgt ttcaatggat gttcgaggtc aagctggaca gtctcaagac aaggggcatt
     3121 ttgatggcat tacagttaag ggccagattg tgcgaggaat gatctcagga ccaaatcatc
     3181 ttttttataa agacatttat cttgatgttt tccaattgat tgatatcatt gctacattag
     3241 agagtgttga tagtaatcaa ctttatagtt atggctggtc tcaaggtgga gcacttgctt
     3301 tgatagctgc agctcttaat cccaaaatcg tcaagaccgt tgctgtttac ccatttttgt
     3361 cagactttag acgagtatta gatttaggga atgtcagtga gccttatgac gaactcttcc
     3421 gctattttaa atatagtgat ccttttcata aaacagaaaa taatgtgtta aaaactttgg
     3481 cttatattga tgttaaaaat tttgcacata ggatttcttg ccccgttgtt ttactaacag
     3541 ccttgaaaga tgatatttgt cctccatcaa gtcaatttgc catttttaac cgtttgacat
     3601 caactaaaaa gcatctttta ttaccagact atggacatga tccaatgaca gtacaagtaa
     3661 aagactatat ttttgatcaa ctgacaggaa gtcaatttac aaagcaaaaa atcgagtaga
     3721 ttatttctac tcgattttca tttagcgttg aataatggtt tctctaaaaa ttcgctcttt
     3781 ttctatttta ttaatatcta aaaattgtgc atagataata tctaccatga ttaacattgg
     3841 gatctgaggc gaaattctat tgccgtagtc taaatgccta gcagaggcca cctgaataat
     3901 cttgtcaaaa gcatagtcaa ttgtgtgagg ttgttttgta aatagaacgg ttttagctcc
     3961 gtgacaactt gcgattttta aggcaccgat aacagagttg gtgttcccag aaagtgagaa
     4021 ggcaatgacg aggcatctat cattaacaat actattagtc catgagaaac tatctgtatc
     4081 atccagagcc tcacaaatca ctcccagcct cataaggcgt attttaaatt ctttagctac
     4141 tagcgatgag ctccctttgc cataaaaata aacacgatca gcttgctcaa tcatatgtga
     4201 tacttctaga agctgttctt catcaataat atcagcggat ttagaaacca tctcttgata
     4261 attcataaat actttcttag tgttgtcatt ttgaagaccg tataattgtt gactgatggt
     4321 ctcatggctt tttaaatatt caaaaacaaa ttcacgatag cctttaaacc cacatttttt
     4381 agcaaaacgc gttaaggctg cttgtgaaat gtgtagttgc ttagtgacaa tggaagaagc
     4441 aagagcgtct tttgaaatgg gctgccccat aaaataacaa gcaatttcat gttctaaatc
     4501 agtcattaaa tcaagagcag actcaatttg gtcataaata gtcataatgc acggtcctct
     4561 cctctttaag aaagcgctta acatattcag tataacataa agttagcgag gggccaaact
     4621 aagttctaag aaacagcaat gataagtttt aaatagcaat atgaaggatt aa
//
LOCUS       DATRQA010000138         4576 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.138, whole genome shotgun sequence.
ACCESSION   DATRQA010000138 DATRQA010000000
VERSION     DATRQA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.138"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(46..921)
                     /gene="tehB"
                     /locus_tag="VBD27_001747"
     CDS             complement(46..921)
                     /gene="tehB"
                     /locus_tag="VBD27_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEO7025736.1"
                     /translation="MVNTKTLLPYKTMPVWTAQSIPKAFLEKHNTKEGTWAKLTILSG
                     SLVFYQLSPDGEEISRHIFDASSDIPFVDPQVWHKVSPNSPDLSCYLTFYCQKEDYFH
                     KKYGLTRTHSEVIASAPLLSEKSNILDLGCGQGRNSLYLSLLGHQVTSVDSNGQSLVA
                     LENMALEEELPYNIKRYDINTAAIEGHYDFILSTVVFMFLNPDCISDIILQMQSHTQI
                     GGYNLIVSAMDTAENPCPLPFPFTFKEGQLKSYYNDWEIIKYNENLGELHRVDENGNR
                     LKLQFATLLARKLRS"
     gene            1064..3061
                     /gene="metG"
                     /locus_tag="VBD27_001748"
     CDS             1064..3061
                     /gene="metG"
                     /locus_tag="VBD27_001748"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO7025737.1"
                     /translation="MTEKKSFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGFD
                     VQYLTGLDEHGQKIQQKAEEAGITPQEYVDGMAESVKTLWELLDISYDKFIRTTDTYH
                     EEAVAKIFEQLLAQGDIYLDEYTGWYSVSDEEFFTESQLAEVYRDENGNMIGGVAPSG
                     HEVEKVSEESYFFRMSKYADRLKAYYAEHPEFIQPDGRMNEMLKNFIEPGLEDLAVSR
                     TTYTWGVQVPSNPKHVIYVWIDALMNYISALGYGWSDDLSQYHKFWPADIHMIGKDIL
                     RFHSIYWPIMLMALDLPLPKRLVAHGWFVMQDGKMSKSKGNVVYPEMLVERFGLDPLR
                     YYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTIAMVNKYFDGEVPRFAVAT
                     DFDADLASVATDSIENYHKQMEAVDFPRALEAVWNLISRTNKYIDETAPWVLAKDETD
                     RDKLAAVMSHLVASLRVVAHLIQPFMMETSDAIMEQLGLGATFDLEKLTFADLPEGVR
                     VVAKGSPIFPRLDMEDEITYIKEQMNAGKAPVEKEWVPEEVELTSSKGQIKFEDFDAV
                     EIRVAEVIEVEKVEGSDKLLRFRLDAGDEGHRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTVLTVDSAVANGSIIG"
     gene            3257..>4576
                     /gene="brnQ"
                     /locus_tag="VBD27_001749"
     CDS             3257..>4576
                     /gene="brnQ"
                     /locus_tag="VBD27_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO7025738.1"
                     /translation="MVKKGFLTGLLLFGIFFGAGNLIFPPALGVASGQDFWPAILGFC
                     LSGVGLAIITLLLGTLTNGGYKTEMSEKFSPWFSLTFLVILYLTIGPLFAIPRTATVS
                     FEIGVAPIVGHSPIALLCFTACFFAAAYYLAIRPNGILDSVGKILTPVFAFLILSLVV
                     VGAIAYGNLESAKASADYAGKAFGSGVLAGYNTLDALAAVAFCLVATETLKKFGFKTK
                     KEYLSTIWIVGIVTSLAFSILYIGLGFLGNKFPVPADILADPNVNKGAYVLSQASYKL
                     FGNFGRYFLSIMVTLTCFTTTVGLIVSVSEFFDKNFRFGNYKLFATVFTLIGFLIANL
                     GLNAVITFSVPVLTLLYPIVIVIVLIILINKWLPLSKKGMSLTIGLVTLVSFVEVLAG
                     QWQEKTLTQLVGFLPFHTISMGWLVPMLIGIVFSLVLSDKQKGQAFDL"
BASE COUNT         1379 a          731 c          924 g         1542 t
ORIGIN      
        1 ttattaccta accaaagata aaagtgagta gatttctact cacttttatg atcttaattt
       61 tcgagctaat aaagtcgcaa actgtaattt taagcgatta ccgttttcat ccaccctgtg
      121 caattcacct aaattttcat tatatttgat gatttcccag tcattgtaat aagatttcaa
      181 ttgtccttct ttgaaggtaa aagggaatgg aagcgggcaa ggattttcag cggtatccat
      241 agcagaaacg ataagattgt atcctccaat ctgagtatgt gattgcattt gtaaaataat
      301 atcagaaatg cagtcgggat ttaaaaacat aaataccaca gttgataaaa taaaatcata
      361 gtgcccttca atagcagcag tattaatatc ataccttttt atattgtaag gaagctcttc
      421 ttctaatgcc atattttcta aagctacaag gctctgtccg tttgaatcga cagaagtcac
      481 ttgatgtccc agcagcgata aataaagtga gtttcgccct tgaccacacc caaggtctaa
      541 tatattactc ttctcagata agagaggtgc actggcgata acctcagaat gtgtgcgcgt
      601 gagaccatat tttttatgga agtaatcttc tttttggcag taaaaagtta gatagcaact
      661 taagtctgga ctattcggcg aaactttatg ccagacttgt ggatcaacaa aaggaatatc
      721 actactagca tcaaaaatat gccgcgagat ttcctctcca tcaggagata actggtaaaa
      781 tactaaagaa ccacttagaa tggttagttt tgcccaggtg ccttccttag tattatgctt
      841 ttctaaaaat gcctttggaa tagactgtgc agtccaaacc ggcatagttt tgtaggggag
      901 aagtgttttt gtattaacca tatattaact ctctattttt aataccttta ttatagcaaa
      961 agtagatata ttattcagag atatatccag ttgaaaaaga ttttatgtta taatgtatca
     1021 tgataaaaaa cacctctcat tagggtggct tataggagaa atcatgacag aaaaaaaatc
     1081 attttacatt actaccccaa tttattaccc atcaggtaaa ttgcacatcg gttctgccta
     1141 tacaacgata gcatgtgatg ttttggcacg ttacaaacgc atgatgggct ttgatgttca
     1201 gtatttgact ggtcttgatg agcatggtca gaaaattcaa caaaaggctg aagaagcagg
     1261 cattacacca caggaatatg ttgatggcat ggctgaaagt gtcaaaacat tatgggaact
     1321 tttagatatt tcctatgaca aattcatccg tacaacagat acttaccatg aagaagcggt
     1381 tgctaaaatt tttgaacaat tgttggcaca aggggacatc tatcttgatg agtataccgg
     1441 atggtattct gtttcagatg aagaattttt tactgaaagc caattagcag aagtttatcg
     1501 tgacgaaaac ggtaatatga ttggtggtgt tgcgccatct ggtcatgaag ttgaaaaagt
     1561 ttctgaagaa tcttatttct tccgcatgag caaatatgca gatcgtttaa aagcgtacta
     1621 cgctgagcat ccagaattta ttcagccaga tggtcgtatg aatgaaatgc ttaaaaactt
     1681 tatagaacca ggtttagaag atttagcagt gtctcgtaca acttacacat ggggagttca
     1741 ggtaccatca aatccaaaac atgttattta tgtctggatt gatgctttga tgaactatat
     1801 ttctgctctt ggttatggtt ggtctgacga tctcagtcaa tatcataaat tttggccagc
     1861 agacattcat atgattggta aagatattct tcgtttccac tctatttatt ggcctatcat
     1921 gttgatggcg ctggatttgc cattaccaaa acgtctagtt gctcatggtt ggtttgtgat
     1981 gcaagatggt aaaatgtcta aatcaaaagg gaatgtcgtt tatccagaaa tgttagtaga
     2041 gcgttttggt cttgatccac tccgttatta tctcatgcgt tcattgcctg ttggttcaga
     2101 cggaactttt acgccagaag attatgttgg acgtattaac tatgaacttg ctaatgattt
     2161 aggaaacctt ctcaaccgca ctattgctat ggttaacaag tatttcgatg gagaagtacc
     2221 aaggtttgct gttgcaactg actttgatgc cgatttagct tctgttgcga cagatagtat
     2281 tgaaaactat cataaacaaa tggaagcggt ggatttccca cgtgcccttg aagctgtatg
     2341 gaatcttatt tcacgaacta acaaatatat tgatgaaaca gcaccgtggg tacttgcaaa
     2401 agacgaaaca gatcgtgata agttagcagc tgtaatgagt catttagttg ctagtctacg
     2461 tgttgttgct cacctcattc aaccgttcat gatggaaact tccgatgcca ttatggaaca
     2521 acttggttta ggggcaacat tcgatcttga aaaattgact tttgctgatc taccagaagg
     2581 tgttcgagtt gttgcaaaag gttcaccaat tttcccaagg ttggatatgg aagacgaaat
     2641 tacttatatc aaagaacaaa tgaatgctgg aaaagcacca gtggaaaagg agtgggttcc
     2701 tgaagaagtt gaattgacat caagcaaagg tcaaatcaag tttgaggatt ttgacgctgt
     2761 tgaaatccgc gttgcagaag taattgaagt tgagaaagtg gaaggttctg ataaacttct
     2821 tcgtttccgt ctagatgctg gtgatgaggg ccatcgtcaa atcttatcag ggattgcaaa
     2881 attttaccca aatgaacaag agttagtagg gaaaaaactc caaattgttg ctaacctcaa
     2941 accacgtaaa atgatgaaaa aatatgtcag tcaaggcatg attctctcag cagaacatga
     3001 tggtaaattg acagtgttga cggtcgattc agcagtggca aatggatcaa ttattggtta
     3061 atcgaaaaga gcattatgct ctttttttgt tttcaagaaa aaagtgactg aatagttgga
     3121 aaattatgaa aatgacctag aatttatttt tatttttgat ataataaaag acatcgattc
     3181 taagcattta gatagaattt gataaggaga atttatggaa agggtgtaaa tagattattt
     3241 acactaatgg ttgcttatgg taaaaaaagg ctttttaacg ggattactct tatttggtat
     3301 cttctttggt gcagggaacc tcatttttcc accagcatta ggtgtagcat ctggacaaga
     3361 tttctggcca gcgattttag gattttgttt gtcaggagtt ggattagcta ttattaccct
     3421 cctactaggc acactaacta atggtggtta taagacagag atgtcggaaa aatttagtcc
     3481 gtggttttct ttaacatttt tagttatact ctacctaact attggtccac tttttgctat
     3541 tcctagaaca gcaacggttt cttttgaaat tggggttgct ccaattgtag ggcatagtcc
     3601 gattgctctt ttgtgtttta cagcctgttt ttttgctgca gcttattatc tcgctattag
     3661 accaaatggt atattagata gtgttggaaa aatcttaacg cctgtttttg cctttttaat
     3721 tctctctttg gttgttgttg gagctattgc ttatggaaac ttggagagtg ctaaggcgag
     3781 tgctgactat gcaggaaaag cgtttggaag tggtgtctta gcaggctata atacacttga
     3841 tgcacttgca gcagttgctt tttgtctagt agcaacagaa acgcttaaaa agtttggttt
     3901 taaaactaaa aaagaatatt tatcaaccat ttggattgtt ggaattgtta ctagtttagc
     3961 atttagtatt ttatatattg gcttaggttt tttagggaat aagtttcctg tcccagctga
     4021 tattttagct gacccaaatg ttaataaagg agcttatgta ttatcacaag catcttataa
     4081 attatttgga aattttggtc gttatttctt gagtattatg gtaacattga catgctttac
     4141 aacaacagtt gggcttattg tgtcagtatc tgaatttttt gataagaact ttcgttttgg
     4201 gaattataaa ttatttgcta ctgtttttac tttgattggc tttttaattg ctaatcttgg
     4261 tttaaatgct gtaattactt tttcagttcc tgtgctaact ttattgtatc cgattgttat
     4321 tgttattgtc ttaattattt tgattaataa gtggttgccg ctttcaaaaa agggaatgtc
     4381 tttgacaatt ggtcttgtca ctttagtgtc gtttgttgag gttctagcgg gacaatggca
     4441 agaaaaaaca ttaactcagt tagttggatt cctccccttt catactattt caatggggtg
     4501 gctagttcca atgttgattg gtatagtatt ttctttggtg ctgtcagata aacaaaaagg
     4561 ccaagcattt gattta
//
LOCUS       DATRQA010000139         4564 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.139, whole genome shotgun sequence.
ACCESSION   DATRQA010000139 DATRQA010000000
VERSION     DATRQA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.139"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            97..1359
                     /gene="purD"
                     /locus_tag="VBD27_001750"
     CDS             97..1359
                     /gene="purD"
                     /locus_tag="VBD27_001750"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO7025739.1"
                     /translation="MKLLVVGSGGREHAIAKKLLVSKGVDQVFVAPGNDGMTLDGLGL
                     VNIVISEHSRLIDFAKENEIAWTFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTYSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKCAY
                     DGDMGPNTGGMGAYAPVPHLPQSVVDTAVETIVKPILEGMITEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENGKALLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIK"
     gene            1645..2133
                     /gene="purE"
                     /locus_tag="VBD27_001751"
     CDS             1645..2133
                     /gene="purE"
                     /locus_tag="VBD27_001751"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO7025740.1"
                     /translation="MQPIISIIMGSKSDWTTMQKTAEVLDNFGIAYEKKVVSAHRTPD
                     LMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSNE
                     LI"
     gene            2120..3193
                     /gene="purK"
                     /locus_tag="VBD27_001752"
     CDS             2120..3193
                     /gene="purK"
                     /locus_tag="VBD27_001752"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO7025741.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLEGLDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAECVLEEFVDFDLEISVIVSGNGKDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKELQLSGTLCVEMFVTADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGQPLPPIKLHDPAVMLNVLGQHVQQAIDYVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFVEDADEVEEF"
     gene            3203..4447
                     /locus_tag="VBD27_001753"
     CDS             3203..4447
                     /locus_tag="VBD27_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEO7025742.1"
                     /translation="MGLFGFGKPKDEALEAKISSLESKLKDKQSILDDLTRQIKVAKD
                     ILKLDDELKKRQSELKALQKEVIVLNDDLDLQEYGFFERKYKFSDSTKYKEELETLRK
                     RQKQLVKDKKAGVIVRPMLLDNSKSKGRAMQNQLIKAMIRGFNGEVDAHLVKVTATNL
                     SKKVDAVVKSFNQLNKMYSRNLVCLSNDYLELKISELQLAVEYELQKLEEKELLREQR
                     AKEREDKKLQAELAAKRKQIEKDRKHFKQMLDNVEELLKTANEEEKEKLNLQLAEYQD
                     KLSELDELEEDIDYREGHATAGYVYIISNIGAFGEDVYKIGVTRRLDPMERIRELGSA
                     SVPFQFDVHALIFSEEAFSLEKELHNRFEKNKINKVNGRKEYFKVPFSDIKAVLEEHK
                     ELAIELTEKAEAFEYRQSLMTK"
BASE COUNT         1397 a          872 c         1083 g         1212 t
ORIGIN      
        1 aaaaacttaa aagcgaacaa atgtgttata attttataaa taatacgttt ttattgcaaa
       61 tttcttttta tattatttaa taggagttat tctgatatga aattgcttgt tgttggttct
      121 ggtggtcgtg agcatgcgat tgctaagaag ttgttagtgt ctaagggtgt agatcaggtt
      181 tttgtggcac ctggtaatga tggtatgacc ttggatggtc taggcttggt aaatatcgta
      241 atttccgaac attctaggct gattgacttt gctaaggaga atgagattgc ttggactttt
      301 attggtcctg atgatgcgct agcggctggt atcgttgatg atttcaacag tgctggactt
      361 agagcttttg ggccaacaaa ggcagcagcg gagctagagt ggtcaaaaga ctttgctaag
      421 gaaatcatgg ttaaatacaa cgttccaaca gcagcctatg gcacatattc agattttgaa
      481 aaagctaaag cctacatcga agagcagggt gcaccaatcg tggtcaaggc tgatggcttg
      541 gcgttaggca agggcgtggt cgtggctgaa accgttgagc aggcggtaga ggcggcgcaa
      601 gagatgcttt tggacaacaa gtttggcgac tcaggagcgc gtgtggttat cgaggaattt
      661 ttggatggtg aagagttctc cctctttgcc ttcgctaatg gcgacaagtt ttacatcatg
      721 ccgacagctc aggatcacaa gtgtgcctat gacggtgata tgggaccaaa cactggcggt
      781 atgggtgcct atgcgccagt tccccacctg cctcagagcg tagtggatac ggcggttgaa
      841 accatcgtta agcctatcct tgagggtatg attactgaag ggcgtcctta tctaggcgtc
      901 ctctatgcgg ggcttatcct gacggctgat ggtccgaagg ttatcgagtt caactcacgt
      961 ttcggtgacc cagaaactca gattatcctc cctcgcctga cttctgattt tgctcagaac
     1021 atcgacgaca tcatgatggg catcgagcct tacatcactt ggcagaagga cggcgtgact
     1081 ttgggcgttg tcgttgcctc agaaggctat ccgctcgatt acgagaaagg tgtgccactg
     1141 cctgaaaaga ccgacggcga catcatcacc tactatgccg gagctaagtt tgcggaaaat
     1201 ggcaaagcac tgctctcaaa cggtggacga gtctatatgc ttgtcaccac agaagacagc
     1261 gtcaaagcag ggcaggacaa aatctatacc caactagccc aacaagacac cacaggcctc
     1321 ttctaccgaa acgacatcgg aagcaaagct attaagtaat aagactactc attcaaatat
     1381 tagcgtcagc aatcagcgac cgaaggtcgc tccaaaaaga aagaagaaag gaactgaaca
     1441 cgggactaat tttctagtga aaaagataaa tcttcctaga tgctaaagca tcttcgtcag
     1501 atttttgcgt cgtaggcgtc tgctgctaaa gcacctagtc gccctgacgt gggtgcgtct
     1561 tcgaaatcaa agatttcgga cttaccgcat atttttctac gaaaattttc gacaagtcga
     1621 aacgtccctt tgtatcttaa tattatgcaa ccaattattt ctattatcat gggttccaaa
     1681 tccgactgga caaccatgca aaaaacggct gaagttctag ataactttgg cattgcttac
     1741 gagaagaaag tcgtctctgc ccaccgtacg ccagacctca tgttcaagca tgctgaggag
     1801 gcgcgtggtc gtggtatcaa aatcatcatt gctggagcag gtggggcagc ccacttgcct
     1861 ggtatggtag cagctaaaac aaccctgcct gttatcggtg tgcctgtcaa atcacgtgcc
     1921 ctttcaggct tggattcgct ctattccatt gtgcagatgc ctggcggtgt gcctgtggca
     1981 accatggcta tcggagaagc gggagcgact aacgctgccc taacagccct ccgtattctc
     2041 tctatcgagg accaaaatct ggctgatgcg ctagctcatt tccatgagga acaaggaaaa
     2101 atcgcagagg agtcaagcaa tgaactcatt taagaccatt gggattatcg gtggtggtca
     2161 gctggggcag atgatggcga ttgcggctat ctacatgggc cacaaggtca ttacgctgga
     2221 tcctgctagc gactgccctg cctcccgcgt tagcgaggtg attgtggcgc cttacgatga
     2281 cgttgaggct ttgggacaat tagctgcgcg ttgtgatgtt ttgacctatg agtttgaaaa
     2341 tgttgatgcc gatggtctgg atgccgttgt gtcagctggt cagctaccgc aggggactga
     2401 tctgctccgc atttctcaaa accgtatctt tgaaaaagac tttctggcaa ataaggctgg
     2461 agtgactgtc gctccctata aggtggtgac gtctagcctt gacctagagg ggcttgactt
     2521 gaccaagacc tatgtcctca agacagcgac aggtggttat gacggtcatg ggcaaaaggt
     2581 tatccgctca gcagaagacc tgccagaggc gcaacaatta gccaactcag ctgagtgtgt
     2641 cttggaagag tttgtcgact ttgaccttga aatctcagtc atcgtgtctg gaaatggcaa
     2701 ggatgtgacg gtctttcccg ttcaggaaaa tatccaccgc aacaatatcc tatcaaaaac
     2761 tatcgtacca gctcgcatct cagaccaact agctgacaag gctaagaaaa tggcggtaca
     2821 gattgccaag gaactccagc tatcaggaac cctctgtgtg gaaatgtttg tgaccgcaga
     2881 tgatatcatc gtcaatgaaa tcgccccacg cccccacaac tcagggcact attctatcga
     2941 agcctgtgac ttttcacagt ttgacaccca catcttgggt gtactggggc aacctctccc
     3001 accaatcaag ctccatgacc cagccgttat gcttaatgtc cttggacaac atgtccagca
     3061 ggcgattgac tatgttgccc aaaaccctag cgctcacctc cacatgtatg gtaaactaga
     3121 agcgaaacat aaccgcaaaa tgggacatgt aacggtgttt gtggaagatg cggatgaggt
     3181 ggaggagttt tagggggaat aaatgggtct atttggattc ggaaagccta aagatgaggc
     3241 tttggaagct aaaatcagta gtttagaaag taagttaaaa gataaacaat ctattttgga
     3301 tgatttaaca aggcagataa aggttgctaa agatatttta aaacttgatg atgaacttaa
     3361 gaaacgccaa tcagaactta aagctttaca aaaagaggta atagttctta atgatgattt
     3421 agatcttcaa gagtacggat tttttgagag aaaatataag ttctcagata gtactaagta
     3481 taaagaagaa ttggaaactc tgagaaagag gcaaaaacag ttagtaaaag ataaaaaagc
     3541 gggtgttatt gtaagaccta tgttgctgga taacagtaaa tcaaaaggac gagcaatgca
     3601 aaatcaactt attaaagcga tgataagagg ttttaatgga gaagttgatg ctcacctggt
     3661 aaaggtaacg gctactaatc taagtaaaaa agtagatgca gttgttaaat catttaatca
     3721 gcttaataag atgtattcaa gaaatttggt atgtctatct aatgactatc ttgagctaaa
     3781 aatttcagag ttacaattag cagtagaata tgaacttcag aaattagaag aaaaagaact
     3841 tcttcgtgaa caacgtgcga aggaaagaga agataagaaa cttcaagcag aattagctgc
     3901 taagagaaaa caaattgaaa aagatagaaa acactttaaa cagatgttag ataacgttga
     3961 agaattactt aaaacagcta atgaggaaga aaaggaaaaa ttaaatcttc aattagcaga
     4021 ataccaagat aaattatcag aactagatga gttagaagaa gatattgatt atcgagaagg
     4081 gcatgcaact gctggatatg tttatatcat ttcaaatatt ggtgcatttg gagaagatgt
     4141 ttacaagatt ggtgttacaa ggcgtctaga tcctatggaa cgtattcgcg aattagggag
     4201 tgcatctgtt ccatttcaat ttgatgtaca tgccttaatt tttagtgagg aagctttttc
     4261 attagaaaaa gagttacata acagatttga aaaaaataaa attaataaag ttaatgggcg
     4321 aaaagagtat tttaaagtcc cattttctga catcaaagca gtgttagagg agcataaaga
     4381 acttgctatc gagttgactg aaaaagcaga agcatttgaa tatagacaaa gtctgatgac
     4441 aaaataatta tatagaaaag actgtatttg tatataacag aatatagttg tagctttgtt
     4501 gaaaagataa ttgtgactta tttgtaggca tctttcattc caaaaattaa tttcaaaaaa
     4561 taaa
//
LOCUS       DATRQA010000140         4535 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.140, whole genome shotgun sequence.
ACCESSION   DATRQA010000140 DATRQA010000000
VERSION     DATRQA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.140"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            70..369
                     /locus_tag="VBD27_001754"
     CDS             70..369
                     /locus_tag="VBD27_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HEO7025743.1"
                     /translation="MVTLTVKELAKNFSNDNKAGEILFEQLKSYFYTDTVVTVSFAGI
                     SEVSSSFVNSAFINLLSYYDFNHIKSQLKIVNSTKQINDLIKQRFSFEISRQITV"
     gene            479..679
                     /locus_tag="VBD27_001755"
     CDS             479..679
                     /locus_tag="VBD27_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7025744.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFVEKTIAKGK
                     ELADDAKDAVEEAVDAVKEKLK"
     gene            832..1254
                     /locus_tag="VBD27_001756"
     CDS             832..1254
                     /locus_tag="VBD27_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEO7025745.1"
                     /translation="MRVLDTLLECLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQS
                     EWVLLTQQLNAEFEGAHLEYLRQNKLYLRKQDKIVTFGKSNKDDFRKTGYNGRGYQPM
                     VYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET"
     gene            1232..1603
                     /locus_tag="VBD27_001757"
     CDS             1232..1603
                     /locus_tag="VBD27_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGG"
                     /protein_id="HEO7025746.1"
                     /translation="MILKKKLKAGILLQAIVLAAVFTLVLQFYLARILATERQYHSQI
                     EASKAYLTAQLAYKTIEGDSISGKCYFTGGYASYLQEGNYLQVKVTLDKGGNYNHKFY
                     RKIDTLQVPLKSNDNKASHKH"
     gene            1718..2692
                     /locus_tag="VBD27_001758"
     CDS             1718..2692
                     /locus_tag="VBD27_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7025747.1"
                     /translation="MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSC
                     DLDIVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQTEQLQANHQFTPDSIGFILLFLL
                     EELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
                     AQFIQEDAVRPQILKESDVIISDLPVGYYPNDDIAKRYAVSSSKEHTYAHHLLMEQSL
                     KYLKKDGIAIFLAPENLLTSPQSDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFV
                     LKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSKNMIE"
     gene            2724..3917
                     /locus_tag="VBD27_001759"
     CDS             2724..3917
                     /locus_tag="VBD27_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO7025748.1"
                     /translation="MSKTIAINAGSSSLKWQLYEMPEEKVVAKGIIERIGLKDSISTV
                     KFDDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEITGVGHRVVAGGEYFKESAL
                     VDDKVVEQVEELSALAPLHNPAAAAGIRAFREILPDITSVCVFDTAFHTTMQPHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHQYVAQEAAKQLGRPLEELKLITAHVGNGVSITANYH
                     GQSIDTSMGFTPLAGPMMGTRSGDIDPAIIPYLVANDPELEDAAAVVNMLNKQSGLLG
                     VSGTSSDMRDIEAGLQSKDPNAVLAYNVFIDRIKKFIGQYLAVLNGADAIIFTAGMGE
                     NAPLMRQDVIAGLSWFGIELDPEKNVFGYFGDITKPDSKVKVLVIPTDEELMIARDVE
                     RLKAK"
     gene            4068..4274
                     /locus_tag="VBD27_001760"
     CDS             4068..4274
                     /locus_tag="VBD27_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7025749.1"
                     /translation="MKNSLQKLRKSRKLSQAELAVALGVTRQTIISLEKEKYTASLEL
                     AFKIARYFDKQIEEVFIYTESEGG"
     gene            4333..4470
                     /locus_tag="VBD27_001761"
     CDS             4333..4470
                     /locus_tag="VBD27_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025750.1"
                     /translation="MSRMWTIDFCPPIVYQQYVFISTISISIAMSIFAIYRYASLKKT
                     I"
BASE COUNT         1619 a          648 c          863 g         1405 t
ORIGIN      
        1 tttttttgaa attcgaatag atgtatcgaa tgacaattta tatgatttag aagaggagga
       61 agaattcgaa tggtaacgtt aactgtcaaa gaactagcaa agaatttttc taacgataat
      121 aaggcgggag agattttatt tgaacaattg aaatcttatt tttatacaga tacagtcgtg
      181 actgtttcgt tcgcaggaat tagcgaagta agttcgtcct ttgtaaactc tgcttttatc
      241 aatttattgt cttactatga ttttaatcat attaaaagtc aactaaaaat tgtaaattca
      301 acaaaacaaa taaatgattt aataaaacaa cgatttagtt ttgaaataag taggcagatt
      361 acagtatagg tgcatattat ttagcaagaa tcgcctgaca ctagcggttc ttgctttttt
      421 atttgcctag aaataatcaa aatgttacca tagaataaaa ataataagga ggcacattat
      481 gtcacaagaa aaactaaaat caaaattaga tcaagcaaaa ggtggtgcta aagaaggctt
      541 tggcaaaata accggtgata aagagttaga agcaaaagga tttgttgaaa aaacaattgc
      601 taaaggcaaa gaactagcag atgatgctaa agatgctgtt gaagaggcag tagatgctgt
      661 caaagaaaaa ctgaaataaa tattaaccgc tcatttaatg agcggttatt ttttgtctat
      721 cagaacagaa aaatttaaaa ttgtctattt ttaggatttt tccgaataaa cctttaggtt
      781 agtgaatgtg tggtgcttga tgaagttatt aaaaaaattt cttcctatta agtgagagtt
      841 ttagatacgc ttttagaatg tttggtagca ttggttacaa tcacaggagc tttgctagtt
      901 tatcaaggac taacaaaatt gttggctcaa cagatagtag tgatgtcttc ttccagtcag
      961 tctgaatggg tgttattaac tcagcaacta aatgcagaat tcgaaggcgc tcacttggaa
     1021 tatttaagac agaacaaact ttatttacgt aagcaagata agattgtaac ctttggcaaa
     1081 tctaataaag atgatttccg taagacaggt tataatggtc gaggttatca accaatggtt
     1141 tatgggttag acaattgtca aatgagtcag accaaaagta tggtaaaact tgttttttat
     1201 tttaaggacg ggttaaaaag gacattttac tatgatttta aagaagaaac ttaaagctgg
     1261 catccttttg caagctattg tattggctgc tgtatttaca ctcgtcttac agttttatct
     1321 agcaagaata ttggcaacag aaagacagta tcatagccaa attgaagcaa gtaaagctta
     1381 tctaacagcg cagttagctt ataaaacaat agaaggcgat tccatatcag gaaaatgtta
     1441 ttttacagga ggatatgctt catacctcca agaagggaac tatttacaag ttaaggttac
     1501 attggataag ggaggaaact ataaccataa gttttatcgt aaaatagaca ctctccaagt
     1561 acctctcaag tctaatgata ataaagcaag ccataaacat taaaaatata cagtgatgaa
     1621 atattagtaa gacaaggttt tgcttaaaaa caggattttt gtttgcttct cttttcagat
     1681 gctgtctttt ttgttatgat aaattctgga ggaaatcatg aattttgaaa aaattgagac
     1741 agcctatgag ctgattttag aaaatatcca aacgattgag aaccaattaa aaactcatat
     1801 ttatgatgcc ttaattgaac agaactctta ttaccttggt tcaagttgtg atttagatat
     1861 tgttgtggtg aataaccaaa aattacgtca acttgactta agtcaagaag aatggcgtcg
     1921 cactttccag ttcattttta tcaaatctgc gcaaacagag caattacaag ctaatcatca
     1981 gtttacgcca gatagtattg gttttatctt gttatttctt ttggaagaat taacgagtca
     2041 agagacagtg gatgtcttgg aaattggaag tggaactggg aatttagctc agactctcct
     2101 caataacagc tcgaaagagt taaattatat gggcattgaa gttgatgatc ttttgattga
     2161 tctatcagca agcattgctg aaattatagg ttctagtgcc caatttatcc aagaggatgc
     2221 cgttagacca caaattttga aagaaagcga tgtaatcatt agtgatttac cagttggcta
     2281 ttatcctaat gatgatattg ctaaacgata tgctgtatca agttctaaag agcacaccta
     2341 tgctcaccat ctattgatgg agcaatctct taaatatttg aaaaaagatg gaatcgctat
     2401 atttttagca cccgaaaacc ttttaacaag tccacaaagt gatttgctga aggagtggtt
     2461 aaaaggatat gcagatgtca ttgccgtttt aactctacca gaaactattt ttggaagtcg
     2521 tcaaaatgcg aaatctatat ttgttctcaa gaagcaagca gaacaaaaac cagaaacctt
     2581 tgtatatccg ctgacagatt tgcaaaatcg tgagaatatg gcaaacttca ttgaaaattt
     2641 tcaaaaatgg agcagagaaa atagtcatta ctcaaaaaat atgatagaat agagattgtg
     2701 aaaacgcttt agagaggtaa attatgtcaa aaacaatagc aattaatgct ggaagttcaa
     2761 gtttgaaatg gcaattatat gaaatgccag aggagaaagt agttgccaaa gggattatcg
     2821 aacgtattgg tttgaaagat tctatttcga ctgtaaaatt tgacgataaa aaagatgaac
     2881 aaattttaga tatcgtagat catacccaag ctgttaaaat tcttttagaa gatctaacaa
     2941 aacatggtat tatcaaagac tttaatgaaa ttacaggtgt tggtcatcgt gtcgttgctg
     3001 gtggtgaata ttttaaagag tctgctttag tagatgataa agttgtagaa caggttgaag
     3061 aattatcagc tttagctcca ctccacaacc cagcagctgc agcagggatt cgtgcgttta
     3121 gagaaatctt accagatatc acgagtgtat gtgtatttga tacagccttc catacaacaa
     3181 tgcaacctca tacctactta tatccaatcc cacaaaagta ctatactgat tataaagtac
     3241 gtaaatacgg tgcacacggg acaagccacc aatacgttgc acaagaagca gctaagcaat
     3301 taggacgtcc tttagaagaa ttaaaattaa tcacagcaca tgtgggtaat ggtgtttcta
     3361 ttacagcaaa ttatcatgga cagtctattg atacatcgat gggattcaca ccacttgcag
     3421 gaccaatgat gggaacacgt tcaggagaca ttgatcctgc gattatccct tatcttgtag
     3481 ctaatgatcc agaattggaa gatgcagctg ctgttgttaa tatgcttaac aaacaatcag
     3541 gattgcttgg agtatcaggc acttcaagtg atatgcgaga tattgaagca ggtcttcaaa
     3601 gtaaagatcc aaatgcagtg ttagcctata atgtctttat tgaccgtatt aagaaattta
     3661 ttggacaata cttggcagta ttaaatggcg cggatgctat tatttttact gcaggtatgg
     3721 gagaaaatgc gccattaatg cgtcaagatg ttatcgcagg attatcatgg tttggcattg
     3781 aacttgatcc agaaaaaaat gtttttggtt attttggaga tatcacaaaa ccagattcaa
     3841 aagtgaaggt tttagttatt cctactgatg aagaattaat gattgcgcgt gacgttgagc
     3901 gccttaaagc taaataagtt atcaaaaaat tgataagacc agaaaagttg atagtttaac
     3961 tatcaacttt tttgtttttt gaacaggtat gtaaaatgta cttgacaaaa aatgtatagt
     4021 taactatact ataaatgtaa agctaactat acaaaaaggt gtaagatatg aaaaattctc
     4081 tacaaaagtt acgaaaatct aggaaattaa gtcaagcaga acttgcagtt gcacttggag
     4141 ttactaggca aactattatt tctctagaaa aagagaaata cacggcatct ttggagctag
     4201 catttaagat agcgcgttat tttgataaac aaattgagga agtttttatt tatacagaaa
     4261 gtgaaggagg atagaatttt aatggaaaaa tacagtccac aaaagacaaa aggttaaaag
     4321 ttaatctgta atatgtcaag gatgtggact attgattttt gtccaccaat cgtttaccag
     4381 caatatgttt ttatcagtac tattagtatt agcattgcga tgtctatttt tgctatttat
     4441 cgctatgctt cattgaagaa aacgatataa tagctgatga ttaaattgaa gttttagaaa
     4501 gtagtattct atgttaataa aaggattatt gtcac
//
LOCUS       DATRQA010000141         4426 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.141, whole genome shotgun sequence.
ACCESSION   DATRQA010000141 DATRQA010000000
VERSION     DATRQA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.141"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            12..318
                     /locus_tag="VBD27_001762"
                     /pseudo
     CDS             12..318
                     /locus_tag="VBD27_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(476..1849)
                     /locus_tag="VBD27_001763"
     CDS             complement(476..1849)
                     /locus_tag="VBD27_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7025751.1"
                     /translation="MLKKLFAPPQKRERLSAEEIKHKYPRYRVQVLISIFVGYMGYYF
                     VRNTTSILSGILNMSATEIGIITCASYIAYGLSKFISGLISDESNSKIFLPVGLFLTG
                     LVNVLIGVIPSVITSIWLFTIMYLINGWLQGMGYPPGARTLVYWYDNKERIKYATIWN
                     LSHNFGGAIAPILTGVGLALAGNDSLNQARAAYWFPGVVACLLAVLVYFLQEDTPESI
                     GLPPIEEYHKEQYTNVVDSSDILEEPEVLGMGEIIKKYILPNTKLMWASLYSIFVYIL
                     RYGIVSWTPKFLATSVQDGGKGITATAGMGGFSLFEIGGIIGMLTAGYLSAKVFKNSK
                     PLTNVAFLVVAILLLAAYWFIPAGPQYMALDFIILLGLGASIYGPVMMVGLYAMELVP
                     KAAAGAASGLTGTFSYVGGATIATLAIGIIIDHFGWGVAFIIFGISGFAAIVCTLLSR
                     DKSLEYW"
     gene            complement(2264..>4426)
                     /gene="glgP"
                     /locus_tag="VBD27_001764"
     CDS             complement(2264..>4426)
                     /gene="glgP"
                     /locus_tag="VBD27_001764"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEO7025752.1"
                     /translation="EAAAKSKNSSQRKVYYISAEFLIGKLLSNNLINLGIYKDVKKEL
                     ELVGKSIAEIEDVEPEPSLGNGGLGRLASCFIDSISSLGINGEGVGLNYHCGLFKQVF
                     RNNQQEAEANYWIENNSWLVPTDISYDVPFRDFTLKSRLDRIDVLGYKKDTKNYLNLF
                     DIDGLDYNLIEKGITFDKTEIKKNLTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLI
                     DEAIERGSNLHDLAEYAYVQINDTHPSMVIPELIRLLTEKHGFEFDEAVSVVRNMVGY
                     TNHTILAEALEKWPLEYLNEVVPHLVTIIKKLDQMIREEQTNPEVQIIDEAGRVHMAH
                     MDIHFSTSVNGVAALHTEILKNSELKVFYDIYPDKFNNKTNGITFRRWLEFANQDLAD
                     YLKELIGDSYLTDATQLEKLLTYADSNEVHDKLAAVKFKNKLALKRYLKENKGIELDE
                     YSIIDTQIKRFHEYKRQQMNALYVIHKYLEIKRGHFPSRKLTVIFGGKAAPAYTIAQD
                     IIHLILCLSELINNDPEVNKYLNVHLVENYNVTVAEKLIPATDISEQISLASKEASGT
                     GNMKFMLNGALTLGTMDGANVEIAELAGKENIYTFGKDSDTIINLYETSGYRSKDYYD
                     KDKVIREAVDFIISDDIVSLGNAERLKRLHDELVGKDWFMTLIDLKEYIAVKEQVLAD
                     YEDYESWNKKVIHNIAKAGFFSSDRTIEQYNQDIWHSN"
BASE COUNT         1521 a          809 c          646 g         1450 t
ORIGIN      
        1 ggaggacttt tatgacttat gaattatgtc tagaatacgg tacttatcca cttagacctg
       61 tagatgcttg ggctgatgaa atcaatactg cacctgcgtt tattactgaa gataaaaaaa
      121 cttttagaac ttttagaaga agtcaatacc ttattccatg agctcttttt aacgatagaa
      181 tgttccttcc actatattgg tcatgacttc ccagaaaaaa gagcaaaaat cacacaaatt
      241 tatcatgtga ttatcgaaca tttaagcata cactaccctg aatatgacat taaaattgaa
      301 tcattattaa tggggtagtt ttattttaac taggtagaaa ctatctatta aaaacaatcg
      361 atatcactaa ttaattcaac aaatagatta tctaaaacaa aaaaggtctc tttcaagagg
      421 ccttttttca taacttatct cactaaatat taaatgatac ttttaccaaa taatatcacc
      481 aatactctag actcttatct cttgaaagca aggtacagac aatagccgca aatccagaaa
      541 taccaaatat tataaatgct actccccaac caaagtggtc aataataata ccaatagcta
      601 gagtagcgat cgttgctcct ccgacataac taaatgtgcc agtcaatcct gaagctgctc
      661 cagctgctgc ttttggaact aactccatag catacaggcc aaccatcata acaggaccgt
      721 agattgatgc ccccaaacct agtaaaataa tgaagtctaa tgccatatat tgaggtcctg
      781 ctggaataaa ccaataggca gctagtaata agattgctac aactaagaag gctacattgg
      841 ttaaaggttt agagttttta aacactttgg cagatagata tcctgctgtt agcatcccaa
      901 taataccacc tatttcaaaa agtgaaaaac cacccatacc agctgttgca gtaatacctt
      961 ttccaccatc ttgcacactc gtcgctaaaa acttaggagt ccatgagaca ataccgtaac
     1021 gtaaaatata tacaaatatt gaatacaagg atgcccacat cagtttagta tttggtagaa
     1081 tatatttttt tataatttcg cccataccca aaacttcagg ctcttctaaa atgtcacttg
     1141 aatccacaac attagtatat tgctctttat gatactcttc tataggcgga agtccgatag
     1201 attctggagt atcttcttgt aaaaagtaaa caagaacagc caaaagacaa gccaccaccc
     1261 ctggaaacca ataagcagca cgcgcttgat taagagaatc attaccagca agagctagac
     1321 ctacaccagt taaaatcggc gctattgctc caccaaaatt gtgagataaa ttccaaatgg
     1381 tagcatattt aattctttct ttattatcat accaatagac taatgtacgc gctccaggag
     1441 ggtatcccat cccttgtaac caaccattaa taagatacat gatagtaaag agccagatac
     1501 tagtaattac agatggaata acaccgatta acacattaac tagtcctgta agaaacaaac
     1561 caactggaag gaaaatttta gaattcgatt catctgaaat caaaccagaa ataaatttag
     1621 agagaccata agcaatgtag ctagcacagg taataatacc aatctcagta gcagacatat
     1681 taaggatacc agataaaatt gaagtcgtat tacgaacaaa atagtatccc atataaccta
     1741 caaaaataga aataaggact tgtacacgat aacgaggata tttatgctta atttcctcag
     1801 ctgataaacg ttctcttttc tgagggggtg caaatagttt tttcaacata gcaaattacc
     1861 tttccgattt ttagatgtgt ttaattatac aactatgaat tcacactgtc aattaattaa
     1921 taatttattt tttatcattt tatcttattt ttttaaatgt ggataatact ctttaaagtg
     1981 tagataatac tatattataa gctttttatc tgatttttct gtattttgaa atcaaattat
     2041 attttttaat tttagtttat atttcaaaaa ttaaaacacc ctataaccaa gtatcacaga
     2101 gtgcatattt ttatattata tagaatataa atacacatgc aattaaatat ttagaataag
     2161 ttctgctcag ttctattttt aaagtctacg gacaaaaata aatacttata gttttattag
     2221 acaagaaaaa agtaaggttg aaaaccttac ttttagagac tttttaattt gaatgccaaa
     2281 tatcttgatt atattgttcg attgtacgat ctgatgagaa gaaccctgct ttagcaatat
     2341 tgtgtataac tttcttattc catgattcat aatcttcata atcagcaagc acttgttctt
     2401 taacagcaat gtattctttc aaatcaatca atgtcatgaa ccaatcttta cctactaact
     2461 catcatgcaa tctctttagt cgttctgcat taccgagtga cactatatca tcactaatga
     2521 taaaatcaac agcttcacga attactttgt ctttatcata gtaatctttt gaacgataac
     2581 cagatgtttc ataaagatta atgatagtat cagagtcttt accaaatgtg tagatgttct
     2641 ctttgccagc taattcagct atttcaacat ttgccccatc catagtacca agagtaagag
     2701 caccattcaa cataaatttc atgttacctg taccagatgc ttctttggaa gccaatgaaa
     2761 tttgttccga aatatctgtt gcagggatta acttttccgc tactgtcaca ttgtaatttt
     2821 ctactaaatg gacattaagg tatttattaa cttctggatc attattaatt aattctgata
     2881 agcagagaat taagtgaatg atatcctgtg caatagtata ggctggagca gctttaccac
     2941 caaaaataac tgttaattta cgtgatggga aatgcccacg tttaatttct aagtatttat
     3001 gaataacata taaagcattc atttgttggc gcttatattc gtggaatcgt ttgatttgtg
     3061 tatcaataat tgagtattca tccaattcaa tacctttatt ttccttaagg taacgtttaa
     3121 gagccagttt gtttttaaat ttaacagcag ctaatttatc atgaacttca ttgctatcag
     3181 cataagttaa caatttttcg agctgagtcg catcagtaag atagctatcc ccaattagtt
     3241 ctttcaagta atctgctaaa tcttggttag caaactccaa ccagcgacgg aatgtaatac
     3301 cgtttgtctt attgttaaac ttatctgggt aaatatcata gaaaaccttt aattcactat
     3361 ttttaaggat ttctgtatgt aatgctgcta caccattaac gcttgttgag aagtggatat
     3421 ccatatgcgc catgtgaaca cgtccagctt catcaataat ttgaacctct ggatttgttt
     3481 gctcttcacg aatcatttga tctaattttt taatgattgt tactaggtgg ggtactacct
     3541 cattaagata ctctaaaggc catttttcaa gagcttctgc aaggatggta tggtttgtat
     3601 agccaaccat attgcgaaca actgacacag cttcgtcaaa ttcaaaacca tgtttttctg
     3661 tcaataaacg aatcaactca gggatgacca tagatgggtg agtgtcatta atttgaacat
     3721 aagcatattc agctaaatca tgaaggtttg aaccacgctc gattgcttca tcaattaata
     3781 attgagcagc atttgagacc atgaaatatt gctgatagat acgtaataat tctccatttt
     3841 tatctgaatc atctggatac aagaacaagg ttaaattctt cttgatttct gtcttatcaa
     3901 aagtgatacc tttttcaata aggttataat caagtccatc aatatcaaaa aggttcaaat
     3961 aatttttagt atctttctta taacctaata catcaattct atctaggcgt gattttaaag
     4021 taaagtcacg gaaaggaaca tcataagaaa tatctgttgg tactaaccaa gaattattct
     4081 caatccagta gttagcttct gcttcttgtt gattgttgcg aaatacttgt ttgaaaagcc
     4141 cacagtgata gtttaaacca acaccttcac cattaatacc taaagaagaa atcgagtcaa
     4201 taaaacatga tgctaaacgc cctaaaccac cattaccaag tgatggttct ggctctacat
     4261 cttctatttc agcaattgat ttacctacaa gttctaactc ttttttgaca tctttataaa
     4321 tccctaagtt aatcaaatta tttgaaagta atttaccaat tagaaactca gctgaaatat
     4381 agtatacctt acgttgagag ctattttttg atttagcggc agcttc
//
LOCUS       DATRQA010000142         4383 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.142, whole genome shotgun sequence.
ACCESSION   DATRQA010000142 DATRQA010000000
VERSION     DATRQA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.142"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(421..873)
                     /locus_tag="VBD27_001765"
     CDS             complement(421..873)
                     /locus_tag="VBD27_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEO7025753.1"
                     /translation="MNRLSWEDYFMANAELISKRSTCDRAFVGAVLVKNNRIIATGYN
                     GGVSETDNCNEVGHYMEDGHCIRTVHAEMNALIQCAKEGISTNNTEIYVTHFPCINCT
                     KALLQAGVKKITYKANYRPHPFAIELMEAKGVAYVQHDVPEVTLGMEE"
     gene            complement(885..1952)
                     /locus_tag="VBD27_001766"
     CDS             complement(885..1952)
                     /locus_tag="VBD27_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO7025754.1"
                     /translation="MGFEQRLTRCQTAISQLSCDALLITNLTNIFYLTGFSGTNATVL
                     ISPKHRIFVTDSRYALIAKNTVREFDIIISREPLAAILKIIRDDALIAIGFETDISYH
                     MYKHMVEVFEDYRLIEAPSVVEKLRMIKDVTELAIIRQACRISDQAFLDVLDFIKPDR
                     TTELQVANFLDFRMRELGATGPSFDFIVASGYRSAMPHGVASQKTIQSGETLTLDFGC
                     YYQHYVSDMTRTIHIGHVTDQEREIYDIVLKSNQAIIDNVKSGMKRCDYDYLARQVIE
                     NSGYGNHFTHGIGHGMGLDVHEIPYFGKSEGVIASGMVVTDEPGIYLDNKYGVRIEDD
                     LLITETGCEVLTSAPKELIVL"
     gene            complement(2052..2768)
                     /locus_tag="VBD27_001767"
     CDS             complement(2052..2768)
                     /locus_tag="VBD27_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEO7025755.1"
                     /translation="MIILIIALATILTFVTWMLIRPKALRYILGLLSLALLFASVWML
                     TDHFINHTGMTIETKTKTREIYSAGDSKAPFGLMIYKEIGTHSSNNVLVYRTDLNQKE
                     AKAHFVPNTKEVTSAVKKSASYQLADVKKATVTTKTKRYVWKDSLSKLLYGFAGENGE
                     LISQKSVAKIPRKTWLVLTKEQSEHLESAAAKLKEQMTSQAKVNPVKAMEIKTLAKTN
                     PEAYAEMQVKALKEALNIKE"
     gene            complement(2770..4269)
                     /locus_tag="VBD27_001768"
     CDS             complement(2770..4269)
                     /locus_tag="VBD27_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO7025756.1"
                     /translation="MTTKAKHDIHGKPYNRTAMITLLLIATFAGVLNQTSLGTAIPTL
                     MNSFNISLSTAQQATTWFLLANGIMIPVSAYLATRFSTKWLYVTSYVVLLIGLLMTTL
                     APTSNWNLFLVGRIIQAISVGISMPLMQVVMVNVFPPEQRGAAMGLNGLVVGLAPAIG
                     PTLAGWILKQEFHFAGHDLTWRAIFLLPLLILTVTTILSPFVLKDVVDNKSVKLEVPS
                     LILSIIGFGSFLWGFTNVATYGWGDIGYVISPIMVGIIFIALFIHRQLKLETPFLDIR
                     VFKNKQFSVTTAAIALSMMAMMGVEMMLPLYLQNVHGLSALDSGLALLPGALMMGIVS
                     PISGAVYDKVGARRMAMIGFTILGVATLPFVFLTTTTPDHFITLLYAVRMFGIAMVMM
                     PLTASAMSALPPHEAAHGTAANNTARQIASAVVVALLSSVAQNIITNNKPSKDLLTMN
                     PLKYANQMLNASLDGFHVSFAIGFVFAVLGLLVSLFLRKGKIIETEKEV"
BASE COUNT         1434 a          884 c          685 g         1380 t
ORIGIN      
        1 ccaatatttt tgatagtttc ttgtccataa ttgatgatat catctttata gttatcatac
       61 acccaatcaa atgccatatt agcataaact ggatttacat ctattgaggt cagcaacaat
      121 atcttttctc ttttttgtaa attatttttg caattcgaga ttcccagcag tactaatttt
      181 agatgttgat aatagatgga tcagctattc tattatcaac atctaaaact ttctgtgaaa
      241 aatcattagg aataatgact attaactggt gatcaccttt tttaggccat tttcagtaat
      301 cccatgacta acaattgacc aattttgaga attatctcgt tcaatatatt taacataact
      361 ggcccttagc ttaaacgata aaaccttcaa ggaagatagg catatactct atcttcctct
      421 ttactcttcc atccctagtg ttacctctgg aacatcatgt tgaacataag caactccttt
      481 tgcttccata agttcaatag caaatgggtg tgggcgataa ttagctttat aagtaatttt
      541 cttaaccccg gcttgcaaga gcgccttggt gcaattgata caaggaaaat gcgtcacata
      601 aatttctgta ttattagtag aaataccttc tttagcacac tgaataaggg cattcatctc
      661 agcatgaaca gtcctaatac agtgaccatc ttccatataa tgtcccactt cgttacagtt
      721 atctgtctca gagactcctc cgttataacc tgtagcaatg atgcgattat ttttgactaa
      781 aacagctcca acaaaagcac ggtcacaggt agaacgttta gaaatcaact ctgcatttgc
      841 cataaaataa tcttcccaag aaagtctatt cattctattt tcctttaaag tacaattaat
      901 tctttcggtg cagatgtcaa tacttcgcaa cctgtttccg ttattaataa atcatcttca
      961 atacgaacac cgtatttatt atcgagatat attcctggct cgtcagtcac taccatccca
     1021 gatgcaatca ctccctctga cttaccaaaa taaggaattt catgaacatc aagccccata
     1081 ccatgcccta taccgtgggt aaaatgatta ccgtatccag aattttcaat gacttgtcgc
     1141 gccaagtaat cataatcaca acgcttcatt cctgatttta cattatcaat aatagcttga
     1201 ttacttttca aaacgatatc gtaaatctcg cgttcctgat cagtgacatg accgatatga
     1261 atcgtacgtg tcatatcact aacatagtgc tgatagtaac aaccaaaatc taaagtcaga
     1321 gtttctcccg attgaatagt cttctgactc gcaaccccat gtggcatcgc tgatcgatat
     1381 ccagatgcta caataaaatc aaatgaagga ccagttgctc ctaattctct catgcgaaag
     1441 tctaaaaaat tagcaacttg caattcagtt gttcgatcag gctttataaa atccaacaca
     1501 tctaaaaaag cttggtccga aatacgacag gcttgacgga tgatcgcaag ttctgttaca
     1561 tctttaatca tacgtaactt ttcaacaaca gatggagctt ctatcaagcg ataatcttca
     1621 aaaacttcca ccatatgttt gtacatatga taagaaatat cagtttcaaa accaatcgct
     1681 atcaatgcgt catcgcgaat aattttcaaa atagccgcaa gtggttctct gctaatgata
     1741 atatcaaact ctcttacagt attctttgct atcaaggcat atcgtgaatc tgtaacaaaa
     1801 atccggtgtt ttggactaat gagtaccgtt gcattagttc ctgaaaaacc agtcaaataa
     1861 aaaatatttg ttaaatttgt tattaagagt gcgtcacatg ataattgtga aattgctgtt
     1921 tgacatctag ttagacgttg ttcaaaaccc ataagaaacc tcgctattct aaccttaatt
     1981 aggttaattt tatcatcttt taaaacattt agctataaga aaagcaactc atttcagttg
     2041 cttttacttt actattcttt tatattaaga gcttctttta atgctttaac ttgcatctca
     2101 gcataagctt ctggatttgt cttagctaat gtctttatct ccatagcttt cactgggttt
     2161 acttttgctt gcgaagtcat ttgttctttt agtttagccg cagccgattc taagtgttcg
     2221 gactgctctt tagtcaaaac tagccaagtt tttcgtggta ttttagcaac tgatttctga
     2281 gaaatcaatt ctccattttc acctgcaaag ccataaagta gtttcgaaag actatctttc
     2341 cacacataac gtttagtctt tgttgttact gtggcttttt tcacatcagc taattggtag
     2401 ctagcagatt ttttcacagc acttgtcact tcttttgtat ttggaacaaa gtgagctttt
     2461 gcttcttttt gatttaaatc tgttcgataa actaaaacat tattactcga atgagtacca
     2521 atctctttat aaatcatgag accaaatgga gccttactat cacccgcact ataaatctca
     2581 cgtgtctttg tcttagtttc aatagtcata cctgtatgat taataaagtg atccgttaac
     2641 atccaaactg aggcaaacaa caaagctaat gagagaagac ctagaatata ccgtagagct
     2701 tttggacgaa tcaacatcca tgtgacaaat gtcaaaatag ttgccagtgc aataatcaaa
     2761 ataatcatgc tatacctcct tttccgtctc aataattttc ccttttctca ggaaaagaga
     2821 taccagtaaa ccaagaaccg caaatacaaa accaatggca aaagaaacat ggaagccatc
     2881 aagactagca ttcaacatct gatttgcata ttttaaagga ttcattgtta gcaaatcttt
     2941 tgatggttta ttgttggtga taatattttg agcaacactt gaaagcaatg caactacaac
     3001 tgctgaagca atttgacgag cagtattatt agctgctgtt ccatgtgcgg cctcatgtgg
     3061 tggaagtgcg ctcatcgcac ttgctgtcaa tggcatcata accatagcaa taccaaacat
     3121 acgtactgca tataagaggg taataaaatg atctggtgtt gtagtcgtta agaaaacaaa
     3181 aggtaacgtc gctacaccta gtatggtaaa accaatcata gccatacgtc ttgcaccaac
     3241 tttatcatat acagctccag aaattggact aactatcccc atcatcaagg cacctggtaa
     3301 taaagctaaa ccagaatcaa gtgcagagag accatgaaca ttttgcaagt aaagcggtag
     3361 catcatttca acacccatca tcgccatcat tgaaagtgca atagctgctg ttgttactga
     3421 gaattgttta tttttgaaaa cacggatatc taagaacggt gtttctagtt ttaattgacg
     3481 atgaatgaat aaggcgataa aaataatacc aaccataata ggagaaataa catatccaat
     3541 atctccccat ccataagttg ccacatttgt gaagccccac aaaaagctac caaagccaat
     3601 tattgaaagg ataagggaag gcacttccaa tttaactgac ttattatcaa ccacatcttt
     3661 tagaacaaag ggggataaaa ttgtagtgac cgttaaaatt agtaaaggaa gaaggaaaat
     3721 tgcacgccat gttaaatcat gccctgcaaa atgaaattct tgttttaaaa tccaccctgc
     3781 taatgtaggc ccaatagctg gggcaagacc aaccaccaaa ccattaagtc ccatagcggc
     3841 accacgctgc tctggaggaa aaacattcac cataacaacc tgcatcaaag gcatagaaat
     3901 ccctacagaa atcgcttgaa taatacgtcc tactaaaaaa aggttccagt tagatgtagg
     3961 tgctaacgtc gtcattaaca gtccaataag taaaacaacg taggatgtca catacaacca
     4021 ttttgttgaa aaacgtgtcg ctaaataagc tgaaacagga atcataatcc cattcgctaa
     4081 taaaaaccat gttgttgctt gttgagccgt tgacaacgaa atgttaaagc tattcatcaa
     4141 agttgggata gccgttccca aacttgtctg atttaatacc cctgcaaatg tagcaatcaa
     4201 caataaggtg atcatggctg tacgattata tggtttgcca tgaatatcat gttttgcttt
     4261 tgtagtcatt ttatctccaa tctctatttc tgattttaac taatatcttt taagaataac
     4321 aaataggtct tataaggtta ttttttgatt ttttaatcaa gaaataatat tattttctga
     4381 aaa
//
LOCUS       DATRQA010000143         4348 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.143, whole genome shotgun sequence.
ACCESSION   DATRQA010000143 DATRQA010000000
VERSION     DATRQA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.143"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..593)
                     /locus_tag="VBD27_001769"
     CDS             complement(<1..593)
                     /locus_tag="VBD27_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7025757.1"
                     /translation="MKNYGKILKELREDKGISLSSLAKSAQLSKSTLSRFENGETQIG
                     IDKFIKALQTLEVGVTINEVSILDSKVKAGTSNTDLEQLTLLESYRDNEDIMRIFSFQ
                     EQQSCDRIESNVLKILAKLFISNLGLNMRLPQDEINLVVTYLNGVTQYNDFYFKVICY
                     FQDILPEDVILNKISNMTKEQLPYSKSLVNLLIKQVI"
     gene            complement(669..2150)
                     /gene="guaB"
                     /locus_tag="VBD27_001770"
     CDS             complement(669..2150)
                     /gene="guaB"
                     /locus_tag="VBD27_001770"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO7025758.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMNTKLADNLTL
                     NIPIITAAMDTVTDSKMAIAIARAGGLGIIHKNMSIVDQAEEVRKVKRSENGVIIDPF
                     FLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQLISEHMT
                     SQNLVTAPIGTDLETAERILHEHRIEKLPLVDDEGRLSGLITIKDIEKVIEFPKAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGY
                     VGAANIKELHDNAQFVEMSGAGLKESHPHDVQITNEAPNYSVH"
     gene            complement(2307..2792)
                     /locus_tag="VBD27_001771"
     CDS             complement(2307..2792)
                     /locus_tag="VBD27_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO7025759.1"
                     /translation="MNKKETRHQLIRSLVSETKVRTQHELRELLEKNGVSVTQATLSR
                     DMKELNLIKVNESSDNATETYYEIHSISQKRWEERLRFYMEDALVMLFPVQNQIVLKT
                     LPGLAQSFGSILDSILLPEILATVCGDDTCLIICNNSEEAQKCFEKLSQYTPPFFFSK
                     K"
     gene            complement(2802..3482)
                     /locus_tag="VBD27_001772"
     CDS             complement(2802..3482)
                     /locus_tag="VBD27_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO7025760.1"
                     /translation="MITKEQYFYFRQLDDFKHFTIEQFDHIVSHIKHRTALKNHTLFF
                     EGDYREKLFLIQSGHVKIEQSDASGSFIYTDYVRQDTVFPYGGLFLDDDYHFSAVAIT
                     DIEYFSLPMALYEEYSLQNINQMKHLCRKYSKLLRVHEIRLRNMVTSSASMRVIQSLA
                     TLLLQVPTERGHLPFPITTIEIANMSGTTRETVSHVLKELRQKDIVEMKGKKLLYNNK
                     NYFKKFIE"
     gene            3690..>4348
                     /locus_tag="VBD27_001773"
     CDS             3690..>4348
                     /locus_tag="VBD27_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEO7025761.1"
                     /translation="MTKFIVDSSYWNLFPTSKIGVILIKDYHMDRNLETELKQLLSDS
                     HSLAKKYLQEKEFSQNKVIQTYRKAYQTFKTKKGARSSIEALLKRINSGNEITSINPL
                     VDIYNAASLRFGLPIGAEDSDTFRGDLKLTITNGGDEFYLIGEDFNIPTLSGELAYVD
                     DVGAVCRCFNWRDGKRTMITDNTQNAFLVIELIDNRREIIFKEALDFIATNTNRFLKA
                     KT"
BASE COUNT         1415 a          811 c          675 g         1447 t
ORIGIN      
        1 ataatgactt gttttatcaa taaattgacc agtgatttag aataaggtaa ctgttcttta
       61 gtcatattac taatcttatt taaaataaca tcttcaggta aaatatcttg aaaatagcag
      121 atgactttga aataaaaatc attgtactgt gtcacaccat tcagataagt aactaccagg
      181 ttaatctcat cttgaggcag cctcatattt aaccctaaat ttgaaataaa aagtttagcc
      241 aatattttga gaacattgct ttctattcta tcacaggatt gttgttcttg gaaactgaaa
      301 attctcatga tatcttcatt atctcgataa gattccagta aagttaattg ctctaaatca
      361 gtattagaag ttccagcctt aacttttgaa tctagaatgg atacttcatt gatagttacc
      421 ccaacctcca acgtttgtaa agctttaata aatttatcaa tacctatttg tgtctcacca
      481 ttttcaaaac gtgatagagt tgattttgat aattgagcac ttttggctag agaacttagg
      541 gatatacctt tgtcttctct caactctttt aaaatttttc cataattttt catacaaaaa
      601 ctttactata tttgtttctt ttatgcaaca aaaagcatcc tcatagggga tgcttttttg
      661 tccttatttt agtgtactga ataatttggt gcctcatttg taatttgaac atcatgggga
      721 tggctttctt tcaaaccagc tcctgacatt tcaacaaatt gagcattatc atgaagttct
      781 tttatgttcg ctgcacctac atatcccata ccagaacgaa tgccaccaag catttggaaa
      841 acaatatcgg ctactgaacc tttgtatgct acacgacctt caataccttc tggaaccaat
      901 ttgttagcct cgtttacaga accttggaaa taacgatcgc ttgaaccttt tttcattgct
      961 gcaattgagc ccataccacg gtatgtcttg aacttacgcc cttggaaaat ttctgtttct
     1021 cctggcgctt catctgttcc tgcaaacatt gaacctagca taactgcatt tccacctgct
     1081 gcaagtgctt ttacaatatc acctgaatat ttaataccac catcagcaat gatagtctta
     1141 ccgtactcac gcgcaacagc cgctgcatcg taaatagctg taatctgagg aacacctacc
     1201 ccagcaacga cacgagtcgt acaaattgaa cctggaccaa taccaacttt aacgacatcc
     1261 acacctgcat cataaagtgc acgcgctcct tctgctgtag caatatttcc cgcaatcaat
     1321 gtgcgattag ggaagtgagc acgaatttca gcaattttac ggagcacacc agctgaatgt
     1381 ccatgtgctg tatcaatgac aatagcatct gcacctgctt caaacaaagc ttctgcacgt
     1441 tcaaaagtat cagaggttac acctactgca cctgcaacta agagacgtcc aaattcatct
     1501 tttgctgcct taggaaattc aataactttt tcgatatcct taatagtaat aagtcctgaa
     1561 agacgtccct catcatctac taaaggtagc ttttcaatac gatgctcatg aagaatacgc
     1621 tccgcagttt caagatctgt accaatagga gctgttacaa gattttgact tgtcatgtgc
     1681 tctgagatta gttgtttata atcagaaatg aaacgcatat cacggttagt aataattcca
     1741 actaatttac gattctcgag tgtctctaca ataggaactc cactaatgcg atagttttgc
     1801 atcaattcct cagcttcaga gacagtatta tctggtgtca agaagaatgg gtcaataata
     1861 acaccatttt ctgagcgttt aactttacga acttcttctg cttgatcaac tattgacata
     1921 tttttatgaa tgataccaag cccaccagca cgtgcaatag caatagccat cttactatct
     1981 gtaactgtat ccattgcagc tgtaatgatt ggaatgttta atgtcaaatt atcagccaac
     2041 tttgtattca tatcaacttc atttggtaaa acatggcttt cagcaggaat aagcagaaca
     2101 tcatcaaaag taaaaccttt tttcaaaaac ttagtatccc aatttgacat caaaaaatcc
     2161 tcttttctta gatagtagat ataaaaaatc ccattaaaat tatattgtta ctatcatatc
     2221 acgctaaaat catttgtcaa tataaaaaga gcatagaatt aatagcttct agctctttaa
     2281 agtaatgaaa aaatcttctt aattttctat ttttttgaga agaagaaagg cggcgtatac
     2341 tgacttaatt tctcaaaaca cttttgagct tcttcactat tattacaaat aatcaaacaa
     2401 gtatcatcac cacagacagt tgctaagatc tcaggtaata aaatcgaatc taatatggag
     2461 ccaaaagatt gagctaagcc aggtagtgtc ttcaaaacga tttggttttg aactggaaac
     2521 agcatgacta gggcatcttc catgtaaaaa cgtaaccgtt cttcccaacg cttctgagat
     2581 atactatgaa tctcataata agtttctgta gcattatcac tgctttcgtt aaccttaatt
     2641 aaattaagtt ccttcatatc tcgagataat gttgcttgcg tcactgaaac gccatttttt
     2701 tctagtaatt cccttaactc atgttgtgta cgcaccttag tctcagagac taaggaacga
     2761 attaattggt gtcttgtttc ttttttattc atataaactc cttattcaat gaatttcttg
     2821 aaataatttt tattattata gagaagcttc tttcctttca tttcaacgat atctttttgg
     2881 cgtaattcct ttaaaacatg actaactgtc tcgcgagtag ttccgctcat attggcaatt
     2941 tcaatcgttg taataggaaa aggtaaatgt cccctttctg taggaacttg caaaagtaga
     3001 gttgcaagcg attggataac tctcatactg gcacttgaag tcaccatatt acgaagtcgt
     3061 atttcatgaa ctcgtaatag cttagaatat ttccgacaaa gatgcttcat ttgattgata
     3121 ttttgaagag agtattcttc gtataaagcc attggcagag aaaaatactc aatatccgta
     3181 atagctactg ctgaaaaatg gtaatcatca tccaaaaaga gacctccata aggaaaaact
     3241 gtatcttgtc taacataatc tgtataaata aaagaaccag aagcgtctga ttgttcaatt
     3301 tttacatgtc ctgactggat taaaaacagt ttttcacggt aatcaccttc aaaaaataag
     3361 gtatgatttt ttagagctgt acgatgttta atgtggctca caatatggtc aaattgttca
     3421 atagtaaagt gtttaaaatc atctagttgt ctgaaataaa aatattgttc ttttgtaatc
     3481 acctgatttt ctagaccttt ctaatcatta gatgcgaaca ttttttgtat aatgaaaatt
     3541 ttttaatgta taaaattttc ttgtcacgat aaatcctcct tcattatagc aaaaaaattc
     3601 gtagaaagaa cgtttttcgt tgaattttga aaacgctttt gagaaaatgt aattgttctg
     3661 atatctttta aaaaaattaa gaggccttca tgactaaatt tattgttgat tcatcgtact
     3721 ggaatttgtt tccaacttct aaaattggag taatcttaat caaagattat cacatggaca
     3781 ggaaccttga aacagaactt aaacagttac tgtctgatag tcattcttta gcaaaaaaat
     3841 atttgcaaga gaaagaattc agtcaaaaca aagttatcca aacttatcgc aaagcatatc
     3901 aaacctttaa aactaaaaaa ggtgcacgtt caagtatcga agcattattg aagcgaatta
     3961 attcaggaaa cgaaattact agtataaatc ctctagttga tatctataat gccgctagcc
     4021 ttaggtttgg attacctatt ggtgctgagg atagtgatac atttagaggt gatttaaaac
     4081 taactattac caatggtggt gatgaatttt acttgattgg cgaagatttt aacataccaa
     4141 ctctttcagg tgagcttgcc tatgttgatg atgttggagc agtttgccgc tgttttaatt
     4201 ggcgtgatgg aaaacgtact atgattaccg ataatactca gaacgctttt ttagttatcg
     4261 aattaattga taatagaaga gaaattattt ttaaagaagc gttggacttt atcgccacca
     4321 atacaaatcg ctttctaaaa gctaaaac
//
LOCUS       DATRQA010000144         4323 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.144, whole genome shotgun sequence.
ACCESSION   DATRQA010000144 DATRQA010000000
VERSION     DATRQA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.144"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            65..2515
                     /locus_tag="VBD27_001774"
     CDS             65..2515
                     /locus_tag="VBD27_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7025762.1"
                     /translation="MVFMANKKKTKGKKTRRPTKAEIERQRAIQRMITALVLTIILFF
                     GIIRLGIFGITVYNVIRFMVGSLAYLFIAATLIYLYFFKWLRKKDSLVAGFLIASLGL
                     LIEWHAYLFSMPILKDKEILRSTARLIVSDLMQFKITVFAGGGMLGALIYKPIAFLFS
                     NIGAYMIGVLFIILGLFLMSSLEVYDIVEFIRAFKNKVAEKHEQNKKERFAKREMKKA
                     IAEQERIERQKAEEEAYLASVNVDPETGEILEDQAEDNLDDALPPEVSETSTPVFEPE
                     ILAYETSPQNDPLPVEPTIYLEDYDSPIPNMRENDEEMVYDLDDDVDDSDIENVDFTP
                     KTTLVYKLPTIDLFAPDKPKNQSKEKDLVRKNIRVLEETFRSFGIDVKVERAEIGPSV
                     TKYEIKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSF
                     RELWEQSDANPENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISS
                     ILMKARPDQVKFMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYE
                     LFSKIGVRNIAGYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIR
                     LGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKL
                     LGRGDMLFKPIDENHPVRLQGSFISDDDVERIVGFIKDQAEADYDDAFDPGEVSETDN
                     GSGGGGGVPESDPLFEEAKGLVLETQKASASMIQRRLSVGFNRATRLMEELEAAGVIG
                     PAEGTKPRKVLMTPTPSE"
     gene            2597..4312
                     /gene="pabB"
                     /locus_tag="VBD27_001775"
     CDS             2597..4312
                     /gene="pabB"
                     /locus_tag="VBD27_001775"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEO7025763.1"
                     /translation="MHIETVIDFKELGKRYRFKNPTKELIADTLEQVLEVIKEVDYYQ
                     SQNYYVVGYLSYEASAAFDSHFKVSQQKLAGEHLAYFTVHKDCENEAFPLSYENVRLA
                     DNWTANVSEQEYQEAIANIKGQIRQGNTYQVNYTLELSQQLCSDPFSVYERLMVEQGA
                     GYNAYIAYDDKRILSVSPELFFKKKDEVLTTRPMKGTSARKPTYQEDVAERDWLANDP
                     KNRSENMMIVDLLRNDMGRICDVGTVKVKKLCQVEQYATVWQMTSTIEGVLSPEVTLM
                     SIFQALYPCGSITGAPKISTMAIINELEKRPRGIYCGTIGLCMPDGQAIFNVPIRTVQ
                     MKGQQAYYGVGGGITWESQTDSEYEETRQKSAVLTRVNPKFQLITTGRVTENKLLFSQ
                     QHVERLVESASYFAYSFDKSKFERELKKYLHQLDEKDYRLKIMLDKTGKVTFEVKQLV
                     NLSKKFLTAEVVVQDYPIKLSPFTYFKTSYRPHIIEGQNEKIFVSPEGLLLETSIGNI
                     VLEKNGRFLTPDLSEGGLNGIYRRHLLKNQKVIEAPLTLKDLESADAIYACNAVRGLY
                     PLNLK"
BASE COUNT         1435 a          660 c          912 g         1316 t
ORIGIN      
        1 ttatgataga atagtatagc atgagttgat agagaaagtg aggaacaagt atttcacttg
       61 aaatatggta tttatggcaa ataagaaaaa aacaaaagga aagaaaacca gaagacctac
      121 taaggcagaa atagagcgtc aaagagctat tcaaaggatg attactgctc ttgttttaac
      181 aattattctc ttctttggta ttatcagatt aggtattttt ggtattacag tctataacgt
      241 catccgtttt atggtaggta gcttggctta cttatttatt gcggcaactt taatctacct
      301 ttatttcttt aaatggttgc gaaagaaaga tagcttagta gcaggttttt tgatagcttc
      361 tttaggatta ttgattgagt ggcatgctta ccttttctca atgcctattt tgaaagataa
      421 agaaattttg cgttcaactg ctcgattaat tgtgtctgat ttaatgcaat ttaaaatcac
      481 tgtttttgcc ggtggaggta tgttgggtgc tttgatttac aagccaattg cttttctctt
      541 ttctaatatt ggtgcctata tgattggtgt tctcttcatc attttgggtc tctttttaat
      601 gagttctctg gaagtttatg acatcgtcga atttattaga gcttttaaaa ataaagtggc
      661 agagaagcac gagcaaaata aaaaggagcg ttttgctaag cgagagatga aaaaagcaat
      721 cgctgaacaa gagcgcatag agcgtcaaaa agctgaagaa gaagcttatt tagcttcggt
      781 taatgtagac cctgaaacgg gtgagattct agaggatcaa gctgaggaca atttggatga
      841 tgcgctacca cctgaggtaa gtgaaacatc aactccggta tttgagccag agatccttgc
      901 ttatgagaca tcgcctcaaa atgatccttt accagtagag ccgacaattt atttagaaga
      961 ctatgattcg ccgattccta atatgagaga aaatgatgag gaaatggttt atgatttaga
     1021 tgatgatgta gatgatagtg atatagaaaa tgtcgacttt acacctaaaa cgacactggt
     1081 ttataaatta ccaacgatag atttatttgc accagataag cctaaaaatc aatccaaaga
     1141 aaaggattta gtccgaaaga atatcagagt tttagaagaa acatttagaa gttttggtat
     1201 cgatgtaaaa gtagaacgtg ctgaaattgg accatcagtt actaaatatg aaattaaacc
     1261 agcagttgga gttcgtgtga atcgtatttc aaatctatct gacgacctag ctcttgctct
     1321 tgcagcaaaa gatgtgcgta tagaagcacc aattcctgga aaatcattaa taggtattga
     1381 agttcctaac tcagaaattg caacggtttc tttccgcgaa ctttgggaac aatctgatgc
     1441 caatcctgaa aaccttttag aagtaccact aggaaaagct gttaacggca atgctcgcag
     1501 ttttaactta gctagaatgc cgcatctttt ggtagctggt tcaactggtt caggtaaatc
     1561 tgtggcagtt aatggaatta tttcaagtat tttgatgaag gcacgtccag atcaagttaa
     1621 gtttatgatg attgatccca aaatggttga attatctgtt tataatgata ttccacattt
     1681 attaatccct gttgtaacca atccgcgtaa agcaagtaag gcactccaaa aagttgttga
     1741 tgaaatggaa aatcgatacg agttatttag caaaattggt gtgcgtaata tagcaggtta
     1801 taatacaaag gttgaagagt ttaatgcttc ctctgagcaa aaacaaatac ctttgccttt
     1861 aatcgttgtc attgtagatg aattggctga cttgatgatg gttgctagta aagaagttga
     1921 agatgctatt attcgtttgg ggcaaaaagc acgtgctgca ggtatccata tgattcttgc
     1981 aactcaacgt ccatccgtag atgttatttc tggtttgatt aaagcaaatg ttccgtcgcg
     2041 tattgcattt gctgtttcaa gtggtactga tagccgtacg atccttgatg aaaatggtgc
     2101 tgaaaagctc ttgggacggg gtgacatgct ctttaagcct attgatgaga atcatccagt
     2161 acgactacaa ggttccttta tttcagatga tgatgttgaa aggatcgttg gttttatcaa
     2221 agaccaagcc gaggctgact atgatgatgc ctttgatcct ggagaagtat ctgaaacaga
     2281 taacggctct ggtggtggcg gcggagtacc tgaaagtgat cctctttttg aagaagccaa
     2341 gggactcgtt ttagagacgc aaaaagcaag tgcctcaatg attcaacgcc gattgtctgt
     2401 tggtttcaat agagcaacaa gactaatgga agaattagaa gcagcggggg ttattggtcc
     2461 agcagaagga accaagccac gaaaagtttt aatgactcca actccgagtg aataagaaaa
     2521 aactaatctg acaagaaatt gtcggattag ttttttatgt tataatgaag caaatagatg
     2581 aagaagaagg ttacgaatgc atattgagac tgttattgat ttcaaagaat taggaaaaag
     2641 atatcgtttt aaaaatccta caaaagaatt aatagctgat actttagaac aagtcttaga
     2701 agtgataaaa gaagttgatt attatcaatc tcaaaattat tatgttgttg gttatttatc
     2761 ttatgaagca tctgctgctt ttgattcaca ttttaaagtt tctcaacaga agttggctgg
     2821 agaacatcta gcttatttta cagtacataa agattgtgag aacgaagctt ttcctttaag
     2881 ttatgaaaat gttagattag cagataattg gactgctaat gtttctgagc aagaatatca
     2941 agaggcaatt gctaatatta aaggacaaat tagacaagga aatacttatc aagtaaatta
     3001 tacactagag cttagccaac aattatgctc ggatcccttt agtgtttatg aacgcctaat
     3061 ggtagaacaa ggagcaggat ataatgctta tattgcctac gacgataaaa ggattttatc
     3121 tgtaagcccg gaattgttct ttaagaagaa ggatgaagtc ttaacaacta gaccaatgaa
     3181 gggaacaagc gctagaaagc ctacttatca agaagatgtg gcggagcgag attggttggc
     3241 aaatgatcct aaaaatcgtt ctgaaaatat gatgattgtt gatttattaa gaaacgatat
     3301 gggccgtatc tgtgatgttg gaacagttaa ggtaaaaaaa ctatgtcaag tggagcagta
     3361 tgcaactgtg tggcaaatga catcaactat tgaaggagtt ttatcgccag aagtgacact
     3421 tatgtctatt tttcaagctc tatatccttg tggatctatc actggagctc ctaagatatc
     3481 aacaatggct attattaatg agttggaaaa acggccaaga ggtatttatt gtgggacgat
     3541 aggactttgc atgccagacg gacaagctat ttttaacgtt cctattcgca cagtacaaat
     3601 gaaaggtcaa caagcctatt acggcgttgg tggaggtatt acgtgggaga gccagacaga
     3661 ttctgaatat gaagaaaccc gtcaaaaatc agctgtttta acacgtgtca atccaaaatt
     3721 tcaattaata actacaggaa gagtgactga aaataaactt cttttctctc aacaacatgt
     3781 cgagagatta gttgaatcag cgtcttattt tgcttattct tttgataaaa gtaaattcga
     3841 aagggaactg aaaaaatacc ttcatcagct agatgagaag gattaccgct tgaaaataat
     3901 gcttgataaa actggtaagg taacgtttga ggtaaaacaa ctagtgaatt tatcaaaaaa
     3961 gtttttaacg gcagaggtgg tcgtacaaga ctaccctatt aaattatccc cgtttactta
     4021 ttttaaaact tcttatcgcc cacatattat tgaaggtcag aatgaaaaga tatttgtatc
     4081 tcctgagggg ttgctattgg aaacaagtat tgggaatatt gttttagaaa aaaatggaag
     4141 gtttttaacc ccagatttat cagaaggagg gttgaatggg atttatcgtc gtcatctcct
     4201 taaaaaccaa aaagtaattg aagcaccact aactttaaaa gatttagaat cagccgatgc
     4261 tatatacgcc tgtaatgctg ttagagggct ttatcctcta aacctaaagt aaaaagagtt
     4321 gga
//
LOCUS       DATRQA010000145         4273 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.145, whole genome shotgun sequence.
ACCESSION   DATRQA010000145 DATRQA010000000
VERSION     DATRQA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.145"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..2594
                     /locus_tag="VBD27_001776"
     CDS             <1..2594
                     /locus_tag="VBD27_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7025764.1"
                     /translation="YTNLMTNIRNGINKVTGNILNTIDKLWQTPVQPITAVNVARRAT
                     KTMQALNEATRLKGRRIGSGQCYALSGWYAKKLDGAWIDSSIGGIRGRIGGGMAAALI
                     GTDYNWGAYGWKLDRSPNAGNLQAGGIYNVKANFGAPFYTTQWGHTGIIKSVSKTRVT
                     VLEQNFVGRMYVVENSYDINSFASGLQTVCYPREIAQGMAVNGATTQQVSGGTQISYE
                     EVVQEAQTESYEEEQIIYIDNSIYKEWKDENGKVEYYLKNGFLYAPLSRDRYPSVLTG
                     NETRDNWIRKDMEIETDSQDVLMSTGLKDLKAHAYPAITYEVDGYVDLEIGDVVRIQD
                     DGYQPPLILTARVIEQVLSKDNPNSNKTKFSNFVEKESQLASDLISDMLRLYDESIPY
                     EIKLATSNGVAFKNGTGESVLTPSLQKNGKDYEAVYFYKNGDSLIDIGPSLIVKASDF
                     NHVLNVTVEAYLNEELVASTQISFTDTEDGADGNTPYWHVAYANSADGTVDFSVSDSA
                     NKRYIGQYTDYDAIGSNDPKKYKWTDMVGTVVVGTNNLIDGTKSFSGEDWFTSATLED
                     ENISNYPFTFKKWISGQKVSHAKDIMVEQGVTYTFSAYVKREVAGNLYFYLYDIADGF
                     ITSDTPRETIIKNVDSSLRRFEITFTPTKTGRIRPRFAMVSSEQGSFSSGGFMLVRGN
                     KTGDWQESEADKASNLDAKADGAFTVEQLNALAERARIAEAELQSKATLDTVNDWVKA
                     LQDEIKAREEGQKLSEQKLIDSSNRMIAVQQTIGEMQVRTDFVNKFMSQSEDGLVIGQ
                     KDGTSSVRVDNDRISFYSSGKEVAYIAQSVLVIDSGIFTTKLQIGRYRIEQYELNPDI
                     NVVRYVG"
     gene            2607..>4273
                     /locus_tag="VBD27_001777"
     CDS             2607..>4273
                     /locus_tag="VBD27_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025765.1"
                     /translation="MTTYYSNSDRSYRLTYIVDEVSTSIADNSSQVRFRLYLTSRTNS
                     YAQYNFSGYAWVGGRYDFSAPSALGFNSNYLLIDKTIKVPHDADGSKTVVVAAKLSGP
                     GGYAPGTLNIPDQKFTLTKIPRTSTVAVSSGYFGDTLNVNINKTNDSFTYDVRYNVNG
                     ITGTVASDISGSTTFKTSLDWASTIPNTTSTPATIYVDTKSNGSVIGTSTGIFYLTVP
                     DNIKPKISSLTLSDTNQKASTIVGANNFVQIISNPVVTFNGASGAYGSTIQNFNAEII
                     GKNQSTQSNGGSLGILNFSGKATVKATVTDSRGRVSDPVTTEINVIPYSPPAFSFTVT
                     RAGAKNDQLVVTRNAKISPLIVDGVQKNKMTLTFKTAPLNTTSFTIDTSNASGTYTTV
                     AELINSTATLSGSYGANKSFDVYGLLSDVFSASGGGTPVKQTVSTESFPLSWHKNSVG
                     IGTLPKIDDTGSLNVAGNIYSDGKQIQQKQLALNNGGAFRHDTTDLNSLQDTGFYCVF
                     KGDNRPSGAGPGYLTVVRHETANYAYQHFYDRTNKTIFTRVLENGAW"
BASE COUNT         1400 a          790 c          931 g         1152 t
ORIGIN      
        1 gctacacaaa tttgatgact aatatccgaa atggtatcaa taaagtaact ggaaatatcc
       61 tcaatacgat tgataagctg tggcaaacac cagtacagcc tataacagcc gtaaacgtag
      121 ctagaagagc tactaagaca atgcaagcac taaatgaagc tactagactt aaaggtcgca
      181 gaatcggctc aggacagtgt tatgctttgt ctggttggta cgctaagaag ttagacggag
      241 cttggattga tagttccatc ggtggtatcc gtggtcgcat tggcggtggg atggctgctg
      301 ctttaatcgg tactgactat aactgggggg cgtatggttg gaagctagac aggtcgccta
      361 atgctggcaa cttgcaagct ggcggtatct ataatgttaa agcaaatttt ggtgctccgt
      421 tttatacaac acaatggggg cacacaggga ttatcaagag tgtgtccaag accagagtta
      481 ctgttttgga gcaaaacttt gttggccgca tgtatgttgt cgaaaactca tatgacatta
      541 actctttcgc atctggattg cagacagtat gctatcctcg tgagattgca caaggaatgg
      601 ctgtcaacgg tgcaacaaca cagcaagtta gtggtgggac acagatatca tatgaggaag
      661 tcgtacaaga agcacagaca gaatcatacg aagaagaaca aatcatctac attgacaact
      721 caatctacaa agagtggaaa gatgaaaacg gtaaagtaga gtactatctc aaaaatggat
      781 ttttgtacgc acctttatcc agagaccgtt atccatctgt tttaacaggt aatgagacac
      841 gggataactg gatacgaaag gacatggaaa ttgagactga tagtcaagac gtcttgatgt
      901 caacaggtct aaaagactta aaagcacacg cctatccagc aatcacttat gaagttgatg
      961 gctatgttga tttagaaatt ggcgatgtcg tacgcattca agatgatggt tatcaaccac
     1021 ctttgatttt gactgctcgt gttattgagc aggttttgtc taaagataat ccaaactcaa
     1081 acaaaactaa atttagtaat tttgttgaga aagaaagtca actagcgtct gatttaatca
     1141 gcgatatgtt gcgtctatac gatgagtcaa ttccgtatga aattaaacta gcgacttcga
     1201 acggtgtcgc ttttaaaaat ggcactggtg aatctgtctt aactccaagt ttgcaaaaaa
     1261 acggaaaaga ctatgaagct gtttattttt acaaaaatgg cgactcatta attgatatcg
     1321 gaccatcact aattgttaaa gcaagcgact ttaaccacgt tttaaacgta acagtagagg
     1381 cgtatttaaa tgaggagctt gtggcaagta cacaaatatc atttacagac acagaagacg
     1441 gtgcagacgg gaatacgcct tactggcatg ttgcttatgc aaatagcgca gatgggacag
     1501 ttgattttag cgtgtctgat agcgcaaaca agcgctacat tgggcaatat actgactacg
     1561 atgcaatagg ctcaaacgac cctaaaaaat ataagtggac tgatatggtt gggacggtcg
     1621 tcgttgggac aaataatcta atcgatggca ctaagtcgtt tagcggagaa gactggttta
     1681 cttctgcaac actagaagat gaaaatatct ctaattatcc atttacattt aaaaaatgga
     1741 taagcggtca aaaggtatca cacgcaaaag acattatggt tgagcaaggt gtgacataca
     1801 cttttagtgc ttatgttaaa cgtgaggtag ctgggaattt atatttttat ctttatgata
     1861 tagcagatgg ttttattact agcgataccc cacgagagac aattataaaa aacgttgact
     1921 ctagtctcag acgttttgaa atcactttta caccaactaa gacaggtagg attagaccaa
     1981 ggttcgcgat ggtgtcatcg gagcaaggta gtttcagctc tggtgggttt atgctcgtta
     2041 ggggaaataa aacaggcgac tggcaggaat cagaagctga caaagctagt aatttagatg
     2101 caaaagccga tggtgctttt acagttgagc aactaaatgc actcgcagaa cgtgctcgca
     2161 ttgcagaagc tgaattgcaa tctaaagcga cactagacac agtaaacgac tgggttaaag
     2221 cactacaaga cgaaatcaaa gcacgagaag aaggacaaaa gctatcagaa caaaaactga
     2281 tagactcttc taatcgcatg atagcagtac agcaaacgat tggtgaaatg caggttcgca
     2341 ccgattttgt caataaattt atgagtcagt cggaagatgg gcttgttatt ggtcaaaaag
     2401 acggtacttc tagtgttcgt gttgataacg accgtataag cttttactca agtggtaaag
     2461 aagtcgctta tattgctcaa agtgtgttag ttattgatag tggtatcttt acgaccaaac
     2521 tacaaatagg gcgctatcgt attgagcaat acgagctaaa tccagatata aatgttgtcc
     2581 gctatgttgg ataggaagga ggttaaatga caacttatta tagtaactct gataggagtt
     2641 accgcttaac ttatattgtt gacgaggtct caacatcaat cgcagataat agcagtcaag
     2701 ttagatttag actttattta acatcaagga ctaacagtta cgcacaatac aactttagcg
     2761 gatatgcttg ggttggtggt agatatgact ttagcgcccc atctgcgctt ggctttaaca
     2821 gtaattattt actgattgat aaaactatca aagtaccaca cgatgctgac ggtagtaaga
     2881 ctgtggttgt cgcagctaag ttgtcaggtc ctggcgggta tgcacccgga acgctaaata
     2941 taccagacca aaaatttacg ctgactaaaa tcccacgaac aagcacagtt gctgtaagta
     3001 gtggttattt tggtgatacg ctaaatgtca acatcaataa aacaaatgac agctttacat
     3061 acgatgtcag atataacgtt aacgggataa ctggtactgt ggctagtgat atatcaggat
     3121 caacgacttt taaaactagt ttggattggg ctagtacgat tccaaataca actagtacac
     3181 cagctacaat ttacgttgac actaaatcga atgggtcggt cattgggaca tcaacaggta
     3241 ttttttatct aactgtacct gataacatca agcctaaaat ttccagcctc actttatcag
     3301 acacgaatca aaaagcatct acaattgtag gtgctaataa ttttgtccag ataatttcta
     3361 atccagtcgt cacatttaat ggggctagtg gtgcttatgg ctctacaatc caaaatttta
     3421 acgctgaaat tataggtaaa aaccaatcta cgcagtcaaa cggtgggtca ttaggcattt
     3481 taaactttag tggtaaagcc acagttaaag caacagtaac ggacagtcgc gggagggtgt
     3541 cagaccctgt gacaacagaa atcaacgtta taccatattc cccaccagct tttagtttta
     3601 ctgtaacacg cgcaggcgct aaaaatgacc aactagttgt cactcgtaac gctaaaatct
     3661 ccccgctgat tgtcgatggc gtccaaaaaa ataaaatgac gctgactttt aaaacagcac
     3721 cacttaatac cacgagtttt acaatagaca catcaaacgc aagcggtact tatactacag
     3781 tcgcagagtt gattaactca acagctacac ttagtggttc gtatggagct aacaaatcat
     3841 ttgatgtcta cggtttgctt agtgatgttt ttagcgcaag tggaggcggt acacctgtta
     3901 aacaaacggt atcaacagag tctttcccgc tgtcatggca taaaaacagc gtcggaattg
     3961 gaacattacc aaaaattgat gatacaggtt ctttaaatgt cgcaggcaat atctattctg
     4021 atggcaagca aatccaacaa aaacaacttg ctttaaataa tggtggggca tttaggcatg
     4081 atacaacaga cttaaacagc ttgcaagaca caggttttta ttgtgttttt aaaggcgata
     4141 acagaccaag tggtgctgga ccgggctatc taacagttgt aagacacgag acagccaatt
     4201 acgcttacca gcatttttat gaccgcacga acaaaaccat ttttacacga gtgctagaaa
     4261 acggggcatg gag
//
LOCUS       DATRQA010000146         4199 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.146, whole genome shotgun sequence.
ACCESSION   DATRQA010000146 DATRQA010000000
VERSION     DATRQA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.146"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            81..848
                     /locus_tag="VBD27_001778"
     CDS             81..848
                     /locus_tag="VBD27_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO7025766.1"
                     /translation="MIDVENILEKMKPNQKINYDWVMQQMVKQWQASDIRPKILMHVC
                     CAPCSTYTLEYLSQWADVTIYFANSNIHPKDEYYRREYVTQKFVHDFNKNTGYSVQFL
                     SAPYEPNEFFKIVHGLEEEPEGGDRCKVCYDFRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINTIGIDVQKIYDTQYLPSDLKKNKGYQRSVEMCKDYDIYRQCYCGCIFGAKD
                     QGINLLQIKKDAKAFVSDKDGKEEFPNIRFTFNGKSM"
     gene            complement(907..2247)
                     /locus_tag="VBD27_001779"
     CDS             complement(907..2247)
                     /locus_tag="VBD27_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO7025767.1"
                     /translation="MYQTQTNKEKFVLFLKLFIPVLIYQFANFSATFIDSVMTGQYSQ
                     LHLAGVSTASNLWTPFFALLVGMISALVPVVGQHLGRGNKEQIRTEFHQFLYLGLILS
                     LILFLIMQFIAQPVLGSLGLEDEVLAVGRGYLNYMLIGIMPLVLFSICRSFFDALGLT
                     RLSMYLMLLILPFNSFFNYMLIYGKFGMPRLGGAGAGLGTSLTYWAIFIVIIIVMSLH
                     PQIKTYHIWTLERIKAPLIIEDIRLGLPIGLQIFAEVAIFAVVGLFMAKFSSIIIAAH
                     QAAMNFSSLMYAFPLSISTALAITISFEVGAERFQDANTYSRIGRLTAVGITSGTLLF
                     LFLFRENVAAMYNSDPHFVAITAQFLTYSLFFQFADAYAAPVQGILRGYKDTTKPFMI
                     GAGSYWLCALPLAVILEKNSQLGPFAYWIGLITGIFVCGLFLNQRLQKIKKLYY"
     gene            complement(2347..2763)
                     /locus_tag="VBD27_001780"
     CDS             complement(2347..2763)
                     /locus_tag="VBD27_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7025768.1"
                     /translation="MVMKAIEIYMNINGRGAEAITFYEEAFNTKAEHVTTFAQGWPDC
                     PPELEHLIMNAQFNIDGIRMQLSDNNPIFDFQIGQNMTAALIVESVEEAQELFNKLSR
                     DAMNIKMPLQAVPWSPAYGNVTDKYGMTWQINTELS"
     gene            complement(2767..3264)
                     /locus_tag="VBD27_001781"
     CDS             complement(2767..3264)
                     /locus_tag="VBD27_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7025769.1"
                     /translation="MIIDFKTVSTKGLETSPVAEALAGLRANEARYFWNKFKVAYETF
                     PVSDYPELMADINHLLKNERNLVFAAKPLEISQLFLENRILWTHVFYEDGLAINILYE
                     LDNPKKRAVGLKLSDGMDIPIELADKFKFARQKSKLAGIIRGSYFTLKGENETLMQRW
                     LDNMK"
     gene            3486..4082
                     /locus_tag="VBD27_001782"
     CDS             3486..4082
                     /locus_tag="VBD27_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEO7025770.1"
                     /translation="MTKKIFLFICATLLVGIALYTPTTSIFNHNNVYAATKKAKIKFN
                     KTQKKIVKKAREYAKSGHMSKDSIIEKLKKDSKKYRQEDINFVINNLKVDYKKNALIS
                     AKIYSKTMNLSKQSIFEQLYSESPDKATHSDKFTKEESQYAIDHLKVDFKENALETAK
                     SYQSSSSLSKEEIYKQLTSTLGDKFTNDEAQYAVDHLK"
BASE COUNT         1507 a          780 c          635 g         1277 t
ORIGIN      
        1 ttgaataaaa ccccaatact cctagtctaa gcaattattt tatgttacac tttaaacaag
       61 tgaattattg aggagttttt atgatagacg ttgagaatat tcttgaaaaa atgaaaccta
      121 accaaaaaat caattacgat tgggtcatgc aacaaatggt taagcagtgg caagctagtg
      181 atatccgtcc gaaaattctg atgcatgttt gctgtgctcc ttgtagtaca tatacccttg
      241 aatacttatc acaatgggca gatgttacca tctattttgc taattcaaat atccatccta
      301 aagatgaata ttatcgccgc gaatatgtta ctcaaaaatt tgttcatgat ttcaacaaaa
      361 atactggata ttctgtacaa ttcttatcag caccatatga accaaatgaa tttttcaaaa
      421 ttgttcatgg gttagaagaa gaacctgaag gtggggatcg ttgtaaagtt tgctatgatt
      481 tccgtcttga taagacagcg gaaaaagccg ttgaactagg ttttgattac tttggcagtg
      541 ccctaactat tagtcctcac aaaaactctc aaacaatcaa tactattggt attgatgtcc
      601 aaaaaattta tgatactcag tatcttccta gcgaccttaa aaaaaataaa ggttaccaac
      661 gttccgttga gatgtgtaaa gattatgata tctaccgaca atgttactgt gggtgtatct
      721 tcggtgctaa agatcaaggg ataaatcttc tccaaatcaa aaaagatgcg aaagctttcg
      781 tgagtgataa agacggcaaa gaagaatttc ctaatatacg cttcacattt aatggcaagt
      841 caatgtaaat gataaaaaaa gacttagtaa agaactaagc ctttttggta taaatcttgt
      901 caaactttaa taatacaact tcttaatctt ttgcagacgt tggtttagaa atagaccaca
      961 aacaaaaata cctgtgatta aaccaatcca ataggcaaac ggacctaact ggctattttt
     1021 ttctaagata accgctaatg gcaaagcaca taaccaataa gagcccgcac cgatcataaa
     1081 tggttttgtt gtatccttat agcctcgtaa aatcccctgt acaggagctg cataagcatc
     1141 tgcaaactgg aaaaagagac tataagttag gaattgagct gtaatagcga caaagtgagg
     1201 gtcactatta tacattgctg ctacattctc acgaaataga aataaaaata gtaaggttcc
     1261 tgatgtaatc cctaccgctg ttaagcgtcc tatcctacta taagtgtttg cgtcctgaaa
     1321 gcgctctgcc cctacttcaa acgatattgt aatagctaga gcagtggaaa tacttaaagg
     1381 aaatgcatac attaatgatg aaaaattcat agcagcctga tgtgctgcaa taatgattga
     1441 agaaaatttt gccatgaata agcctactac tgcaaaaatt gcaacttctg caaaaatttg
     1501 taaaccaatc ggtaatccca atcgaatatc ttcaataatc aaaggagctt ttattctttc
     1561 cagagtccat atatgatatg ttttaatttg aggatgaagt gacatcacaa taataataac
     1621 aataaaaata gcccaataag ttaaagaagt tccaagacct gcccccgcac ctcctagtct
     1681 aggcatacca aatttaccgt agataagcat ataattaaaa aatgaattaa agggtagaat
     1741 taaaagcatc agatacatag ataaccttgt taaccccaat gcatcaaaga atgaacggca
     1801 aatgctaaac aacaccagcg gcatgattcc aatcaacata taatttaaat aaccacgacc
     1861 aactgctaga acttcatctt ctaaacccaa actccccaag acaggttgag caataaattg
     1921 catgattaaa aataatatta aggacagtat caaacctaaa tatagaaatt gatgaaattc
     1981 tgtgcgaatt tgttctttat ttcctctacc caaatgttga ccaactactg gtactaatgc
     2041 tgaaatcata cctactaata aagcgaaaaa cggagtccat aaattactag cagttgacac
     2101 acctgccaaa tgtagctgac tatactgtcc agtcataacc gaatcaataa aagtagctga
     2161 aaaattagca aattgataaa tcaatactgg gataaataat ttcaaaaata aaacaaattt
     2221 ttccttattt gtctgagttt gatacatctt ttttctcctt ttcaccttaa tcaatttatt
     2281 ttagcttagc taggatactc tgtcaatgct atttaaaaag gttgagaaat aatcctcaac
     2341 cccattttag ctaagctcag tattgatttg ccatgtcata ccatatttgt cagtcacatt
     2401 tccataagct ggactccacg gaactgcttg tagaggcatt ttaatgttca ttgcatctcg
     2461 agaaagctta ttgaataact cttgcgcttc ttctacagat tccacaatta aagctgctgt
     2521 catattttgt ccaatttgaa aatcaaaaat aggattattg tcagaaagtt gcattcgaat
     2581 accatcaata ttaaattgag cgttcataat taaatgttca agttctggcg gacaatctgg
     2641 ccaaccctgt gcaaaagttg tgacgtgttc tgcttttgta ttaaaagcct cttcataaaa
     2701 tgtaatagct tcggccccac gaccattaat attcatataa atttcaattg ctttcattac
     2761 catacctcac ttcatattat ccaaccaacg ctgcatcaga gtttcatttt ccccttttag
     2821 ggtaaaataa gaaccacgta ttatacctgc gagttttgac ttttgacgag caaacttaaa
     2881 cttgtccgct aattcaatag gaatatccat tccatctgat aattttaaac ctacagctct
     2941 ctttttagga ttatctaatt catataaaat attaatggct aaaccatcct catagaaaac
     3001 atgtgtccat aaaatacgat tctctaaaaa aagctgtgaa atctctaaag gctttgcagc
     3061 aaaaacaagg ttacgttcat tttttagaag gtgattaata tctgccatca attctggata
     3121 gtctgatact ggaaatgttt cgtaggctac tttgaattta ttccagaaat agcgagcttc
     3181 atttgcacgc aaaccagcca aggcttctgc cactggagaa gtctccaaac ctttagttga
     3241 aacggtttta aagtcgataa tcatgttctt tcctttttga ttaaaatctt cgataattta
     3301 gtatactctt ttttgaagaa aaagtacatt ctaatcactt ataaaaagta gtacatttag
     3361 aacactacca cctcagtaat ttcttgacct ttctctactt aatacttata attaatctag
     3421 acttaatgtc aaagcatcaa tcaaggtgta aacctgattc acctaaaaag ctgatggagc
     3481 taaacatgac caaaaagata ttcttattta tctgcgctac tcttttagta ggaatagcct
     3541 tatatacacc aacaacttct atttttaatc ataacaacgt ttatgctgcc accaagaaag
     3601 caaaaattaa atttaataaa actcaaaaaa aaattgtcaa aaaagcaaga gaatatgcta
     3661 agtctggcca tatgtctaaa gatagtatca ttgaaaaatt aaaaaaggat tctaagaaat
     3721 atcgtcaaga agacatcaac tttgtgatta ataacctcaa ggttgattac aaaaaaaatg
     3781 ccttgatttc tgccaaaatt tattcaaaga ccatgaattt atctaaacaa tctatctttg
     3841 aacagctcta ttcggagtct ccagataagg caacccactc tgataaattt acaaaagaag
     3901 aatcccaata tgctatagac catctcaaag tagatttcaa agaaaatgct cttgagactg
     3961 ctaaaagtta tcaaagtagt tcttctttat ccaaagaaga aatctataaa caattgacat
     4021 ctacgctggg cgataaattc acaaatgatg aagcccaata tgctgtagat catttgaaat
     4081 gatgacatac caatagtcta tccttatgta atagaattga tagcttatgc taatattcca
     4141 gctgtaaaat agcaagaacc ctcctaagtt cattaagact taggagggtt tttatcaat
//
LOCUS       DATRQA010000147         4141 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.147, whole genome shotgun sequence.
ACCESSION   DATRQA010000147 DATRQA010000000
VERSION     DATRQA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.147"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            87..2126
                     /locus_tag="VBD27_001783"
     CDS             87..2126
                     /locus_tag="VBD27_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7025771.1"
                     /translation="MKRKYFVLNTVTVLTLATAMNTSSIYANSTETSASVAPTTNTIV
                     QTNDSNPTAKFASESGQSVIGQVKPANSAALTTVDTPHISAPDALKTTQSSPVVESPS
                     TKLTEETYKQKDGQDLANMVRSGQVTSEELVNMAYDIIAKENPSLNAVITTRRQEAIE
                     EARKLKDTNQPFLGVPLLVKGLGHSIKGGETNNGLIYADGKISTFDSSYVKKYKDLGF
                     IILGQTNFPEYGWRNITDSKLYGPTHNPWNLAHNAGGSSGGSAAAIASGMTPIASGSD
                     AGGSIRIPSSWTGLVGLKPTRGLVSNEKPDSYSTAVHFPLTKSSRDAETLLTYLKKSD
                     QTLVSVNDLKSLPIAYTLKSPMGTEVSQDAKNAIMDNVTFLRKQGFKVTEIDLPIDGR
                     ALMRDYSTLAIGMGGAFSTIEKDLKKHGFTKEDVDPITWAVHVIYQNSDKAELKKSIV
                     EAQKHMDDYRKAMEKLHKQFPIFLSPTTASLAPLNTDPYVTEKDKRAIYNMENLSQEE
                     RIALFNRQWEPMLRRTPFTPIANMTGLPAISIPTYLSESGLPIGMMLMAGANYDMVLI
                     KFATFFEKHHGFNVKWQRIIDKEVKPSADLIQPTNSLFKAHSSLVNLEENSQVTQVSI
                     SKKWMKSSVKNKPSVMAYQKALPKTGDTESSLSPVLVVTLLLACFSFVTKKNQKS"
     gene            2236..2814
                     /locus_tag="VBD27_001784"
     CDS             2236..2814
                     /locus_tag="VBD27_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7025772.1"
                     /translation="MKVKNKILTMVALTVLTCATYSSIGYADTSDKNTDTSVVTTTLS
                     EEKRSDELDQSSTGSSSENESSSSSEPETNPSTNPPTTEPSQPSPSEENKPDGSTKTE
                     IGNNKDISSGTKVLISEDSIKNFSKASSDQEEVDRDESSSSKANDEKKGHSKPKKELP
                     KTGDSHSDTVIASTGGIILLSLSLYNKKMKLY"
     gene            complement(2848..4089)
                     /locus_tag="VBD27_001785"
     CDS             complement(2848..4089)
                     /locus_tag="VBD27_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEO7025773.1"
                     /translation="MVLQDFDNLLKKYAQLIISKGLNVQKGHTLALTIDVEQVHLARL
                     LTEVAYEKGASEVIVDYTDDFITRQRLLHASDEVLTNVPQYTVDKSLALLNKKASRLV
                     VKSSNPNAFATVDPKRLSKTTRATAIALEEQSRAIQANKVSWNVAAAAGREWAALVFP
                     ELKTSDQQVDALWDTIFKLNRIYEDNPIAAWDAHEAKLLEKATRLNQEQFDALHYTAP
                     GTDLTLGMPKNHIWEAAGSLNAQGETFIANMPTEEIFSAPDYRRADGYVTSTKPLSYA
                     GVIIENMTFTFKDGKIINVTAEKGQETIQRLIEENDGARSLGEVALVPHKTPISLSGL
                     IFFNTLFDENASNHLAIGSAYAFNVEGGTEMTSQELDEAGLNRSSTHVDFMIGSEQMD
                     IDGIRADGTAVPIFRNGEWAI"
BASE COUNT         1409 a          734 c          753 g         1245 t
ORIGIN      
        1 aatataaaat aacagttaat attttagcaa tttgataaca cttagattat aatgtaaacg
       61 tatacttttt tagattggaa gatgaaatga aacgtaaata ctttgttctt aatacggtga
      121 cggttttaac gttagctact gcaatgaata ctagcagtat ctatgctaat agtactgaga
      181 caagtgcttc agtagctcct actacaaata ctatcgttca aactaatgac agtaatccta
      241 ccgcaaaatt tgcatcagaa tcaggacaat ctgtaatagg tcaagtaaaa ccagctaatt
      301 ctgcggcgct tacaacagtt gacacgcctc atatttcagc tccagatgct ttaaaaacaa
      361 ctcaatcaag tcctgtcgtt gagagtcctt ctactaagtt aactgaagag acatacaaac
      421 aaaaagatgg tcaagattta gccaacatgg tgagaagtgg tcaagttact agtgaggaac
      481 tcgtcaatat ggcatacgat attatcgcta aagaaaaccc atctttaaat gcagtcatta
      541 ctactagacg ccaagaagcc attgaagagg ctagaaaact taaagatact aatcagccgt
      601 ttttaggtgt tcccttgtta gtcaaggggt tagggcacag tattaaaggt ggtgaaacca
      661 ataatggctt gatctatgca gatggaaaaa ttagcacatt tgacagtagc tatgtcaaaa
      721 aatataaaga tttaggattt attattttag gacaaacgaa ttttccagag tatgggtggc
      781 gtaatataac agactctaaa ttatacggtc caacgcataa tccttggaat cttgctcata
      841 acgctggtgg ctcttctggt ggaagtgcag cagctattgc tagcggaatg acgccaattg
      901 ctagcggcag tgatgctggt ggttctatcc gtattccatc ttcttggacg ggcttagtag
      961 gtttaaaacc aacaagagga ttggtgagta atgaaaagcc agattcgtat agtacagcag
     1021 ttcattttcc attaactaag tcatctagag acgcagaaac attgttaact tacctaaaga
     1081 aaagcgatca aacgctagta tcagttaatg atttaaaatc tttaccaatt gcttatactt
     1141 tgaaatcacc aatgggaaca gaagttagtc aagatgctaa aaatgctatt atggacaacg
     1201 tcacattctt aagaaaacaa ggattcaaag tgacagagat agatttacca attgatggta
     1261 gagcattaat gcgtgattat tcaaccttgg ctattggcat gggaggagct ttttcaacaa
     1321 ttgaaaaaga cttaaaaaaa catggtttta ctaaagaaga cgttgatccc attacttggg
     1381 cagttcatgt tatttatcaa aattcagata aggctgaact taagaaatct attgtggaag
     1441 cccaaaaaca tatggatgat tatcgtaagg caatggagaa gcttcacaag caatttccta
     1501 ttttcttatc gccaacgacc gcaagtttag cccctctaaa tacagatcca tatgtaacag
     1561 agaaagataa aagagcgatt tataatatgg aaaacttgag ccaagaagaa agaattgctc
     1621 tctttaatcg ccagtgggag cctatgttgc gtagaacacc ttttacacca attgctaata
     1681 tgacaggact cccagctatc agtatcccga cttacttatc tgagtctggt ttacccatag
     1741 ggatgatgtt aatggcaggt gcaaactatg atatggtatt aattaaattt gcaactttct
     1801 ttgaaaaaca tcatggtttt aatgttaaat ggcaaagaat aatagataaa gaagtgaaac
     1861 catctgctga cctaatacag cctactaact ccctctttaa agctcattca tcattagtaa
     1921 atttagaaga aaattcacaa gttactcaag tatctatctc taaaaaatgg atgaaatcgt
     1981 ctgttaaaaa taaaccatcc gtaatggcat atcaaaaagc acttcctaaa acaggtgata
     2041 cagaatcaag tctatctcca gttttagtag taaccctttt attagcttgt tttagctttg
     2101 taacaaaaaa gaatcagaaa agttgattgt aatgtaaaat aattcctatt tttcactttt
     2161 tagtatcata ttttcgattt ttatgttgta ataaattata atgaagatgt aaggaattat
     2221 aaaataggaa ataatatgaa agtgaaaaat aagattttaa cgatggtagc acttactgtc
     2281 ttaacatgtg ctacttattc atcaatcggt tatgctgata caagtgataa gaatactgac
     2341 acgagtgtcg tgactacgac cttatctgag gagaaaagat cagatgaact agaccagtct
     2401 agtactggtt cttcttctga aaatgaatca agttcatcaa gtgaaccaga aacaaatccc
     2461 tcaactaatc cacctacaac agaaccatcg caaccctcac ctagtgaaga gaacaagcct
     2521 gatgggagca cgaagacaga aattggcaat aataaggata tttctagtgg aacaaaagta
     2581 ttaatttcag aagatagtat taagaatttt agtaaagcaa gtagtgatca agaagaagtg
     2641 gatcgcgatg aatcatcatc ttcaaaagca aatgatgaga aaaaaggcca cagtaagcct
     2701 aaaaaggaac ttcctaaaac aggagatagc cactcagata ctgtaatagc atctacggga
     2761 gggattattc tgttatcatt aagtctttac aataagaaaa tgaaacttta ttaaatataa
     2821 aaaggaggac taacctcctt tttatattta aatagcccat tcgccatttc taaagattgg
     2881 gacagcagtt ccatctgcac gaataccatc aatatccatt tgttctgaac caatcataaa
     2941 atcaacatgt gttgaagaac gatttaaacc agcttcatcc aattcttgac ttgtcatttc
     3001 tgttcctcct tctacattga aggcatatgc agatccaata gcgaggtgat tagaggcatt
     3061 ttcatcgaat aaagtattaa agaaaattag tccagacagt gaaattggtg ttttatgtgg
     3121 gacaagtgca acttccccaa gcgatcttgc cccatcattt tcctcgatca agcgttggat
     3181 tgtttcttgc cctttttctg cagtgacatt gataatttta ccgtctttaa aagtaaacgt
     3241 catattttcg ataataacgc cagcataact gagaggtttt gtacttgtca catacccatc
     3301 tgcacgacgg taatcaggtg ctgaaaagat ttcttcagta ggcatattag cgataaaagt
     3361 ctctccctga gcgttgagac taccggctgc ctcccaaatg tgatttttag gcataccaag
     3421 cgttaaatct gtacctggtg cggtataatg aagagcatca aattgttctt gatttagtct
     3481 agtagctttt tctaataatt tagcttcatg tgcatcccaa gcagcaatag gattatcttc
     3541 ataaatacga tttaatttga aaatggtatc ccaaagagca tcaacttgtt ggtcgcttgt
     3601 ttttaattct gggaagacaa gtgcagccca ttctctacca gcagctgcag ccacgttcca
     3661 agatacttta ttagcttgta ttgctctact ttgttcctct aaggcaatag cggttgctct
     3721 agttgtttta gataaacgtt taggatcaac agtagcgaaa gcgttagggt tagaagattt
     3781 cacaactaat cgactagcct tcttatttaa tagcgctaaa gatttatcaa cggtatactg
     3841 tggaacattc gtgagaactt cgtctgaagc atgaagtagt cgctggcgcg tgataaaatc
     3901 atctgtataa tcaacaataa cttcacttgc tcccttttca taagcgactt cagttaaaag
     3961 ccttgctaag tggacttgtt ccacatcgat tgttaaagcg agagtgtgcc ctttttggac
     4021 atttaaacct ttagaaataa ttaattgggc atattttttt aaaaggttgt cgaaatcttg
     4081 taaaaccata ctagctcctt taaatttact attttttata atttatcata aactcttata
     4141 a
//
LOCUS       DATRQA010000148         4129 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.148, whole genome shotgun sequence.
ACCESSION   DATRQA010000148 DATRQA010000000
VERSION     DATRQA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.148"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(60..1664)
                     /locus_tag="VBD27_001786"
     CDS             complement(60..1664)
                     /locus_tag="VBD27_001786"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO7025774.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLKKE
                     RRGEYLGATVQVIPHVTDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QSIKNKLAQFCDVAPEAVIESLDVDHIYQIPLNMQAQNMDQIVCDHLKLETPAADMTE
                     WSAMVDKVMNLEKKVKIALVGKYVELPDAYLSVVEALKHSGYVNDVAIDLKWVNAAEV
                     TEDNIKELVGDADGIIVPGGFGQRGSEGKIEAIRYARENDVPMLGVCLGMQLTCVEFA
                     RNVLNLHGANSAELDPKTPFPIIDIMRDQIDIEDMGGTLRLGLYPCKLKAGSRAAAAY
                     NNQEVVQRRHRHRYEFNTKFREQFEAAGFVFSGVSPDNRLMEVVELPEKKFFVAAQYH
                     PELQSRPNHAEELYTAFVTAAVENMK"
     gene            complement(1937..2521)
                     /locus_tag="VBD27_001787"
     CDS             complement(1937..2521)
                     /locus_tag="VBD27_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEO7025775.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILEQRGRDNEMYFSDLVNDIQT
                     YLGKSDSAIRESLPFFYSDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     APKRKKKRVNAFMDGDEDAIDYNDDDPEDEDFTEETPSLEYDEENPDDEKSEVESYDS
                     EINEIIPDEDLDEDVEINEEEEEEEEEEEEEEEV"
     gene            complement(2710..3993)
                     /locus_tag="VBD27_001788"
     CDS             complement(2710..3993)
                     /locus_tag="VBD27_001788"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO7025776.1"
                     /translation="MSTSFENKATNRGIITFTISQDEIKPALDQAFNKVKKDLNVPGF
                     RKGHMPRTVFNQKFGEEALYENALNLVLPKAYEAAVAELGLDVVAQPKIDVVSMEKGQ
                     DWKLTAEVVTKPEVKLGDYKDLSVEVDASKEVSDEEVDAKVERERNNLAELTVKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAGQTVDVNVT
                     FPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDDEVETLDELKAKYRKEL
                     ESAKEIAFDDAVEGVAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEM
                     YFQLTGTTEEDLHKQYQADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYN
                     MEADQVRGLLSADMLKHDIAMKKAVDVITSSATVK"
BASE COUNT         1212 a          884 c          638 g         1395 t
ORIGIN      
        1 ttgatagtga aactatctaa aaaaataaaa tcgcctccta atactgggag gcgataaagt
       61 tatttcatat tttcaactgc tgctgttaca aaagctgtgt aaagctcttc agcatggtta
      121 ggacgtgatt gtagttctgg atggtattgt gctgcaacaa agaatttttt ctctggtaac
      181 tcaacaactt ccatcaaacg attatccggt gatacccctg agaagacaaa tccagcagcc
      241 tcaaattgct cacggaattt tgtattaaat tcataacggt gacggtgacg acgttggaca
      301 acttcttgat tattataagc tgccgcagct cttgaacctg ctttcaattt acatggatat
      361 agaccaaggc gaagtgtacc acccatatct tcaatatcga tttgatcacg catgatatca
      421 atgattggga acggtgtttt agggtcaagc tctgctgaat tagctccatg aaggtttaaa
      481 acattgcgcg caaattcgac acaagttagc tgcattccga gacaaacacc aagcattgga
      541 acatcatttt cacgtgcata gcgaattgct tcaattttac cctcactacc tctttggcca
      601 aatccaccag gaacgatgat accatctgca tctccgacaa gttctttaat attatcttct
      661 gtcacttcag cagcatttac ccattttagg tcaatagcaa catcgttgac gtaccctgag
      721 tgcttaaggg cctccacaac tgaaaggtaa gcatcaggta attcaacata tttaccaacc
      781 aatgcaattt taactttttt ctcaaggttc attactttat caaccattgc tgaccattct
      841 gtcatatcag cagcaggtgt ctcaagcttc aagtgatcac aaacaatttg atccatattt
      901 tgagcttgca tgttaagtgg aatttgataa atatggtcaa catcaaggga ctctataaca
      961 gcctctggtg caacatcaca aaattgtgcc aatttattct taatactttg acctgctggt
     1021 tgctctgtac ggataacaag catattaggt tggataccta ggccacgtaa ctctttaaca
     1081 gaatgttgag tcggttttgt tttcatttcg ccggcagcct ttaggtatgg taataacgtt
     1141 gtatgaatgt acataacatt atcagaacca acatctgcct tcatttgacg aagtgcttct
     1201 aaaaacggaa ggctctcaat atctccaaca gtaccgccaa cttcagtaat aataacatct
     1261 gaatctgtag ttgtcgcagc acgtttgatt ttttctttca aagcatccgt aacgtgtggg
     1321 ataacttgaa cagttgcacc aaggtattct ccacggcgtt cttttttaag aacttcacta
     1381 taaatcttac ccgttgtaac atttgaatac ttgtttaagt taatatcaat aaaacgctcg
     1441 tagtgtccaa ggtcaaggtc agtttctgca ccatcatctg taacataaac ttccccatgt
     1501 tgataagggc tcattgtccc tgggtcaata ttaatatatg gatcaaattt ttgaattgtc
     1561 acctttagcc cacggttttt tagtaagcgc ccaagacttg ctgcaacaat ccccttacca
     1621 atagaagaga ccacaccacc tgtaacaaaa atatactttg tcattaaaaa ctccttaaat
     1681 taaatgttaa tctaacagtt agtttacggg taaaacccaa aacacatgat tgataaataa
     1741 taccagcttc tctagtcaaa actagtagaa actatctgat caagggccta ctcagaaaac
     1801 tatctggaac cattctgtat ctttcccaaa aacaaaatag ccccctatca ctagggagct
     1861 ttatctgacc tcataagagg tgcccgaata atagtttaaa ctatatctga ctatttgtca
     1921 aatacaaaat ccccatctaa acctcttctt cctcttcttc ctcttcttcc tcttcttcct
     1981 cttcttcgtt gatttctaca tcctcatcta aatcttcatc aggaatgatt tcattaatct
     2041 cagaatcata cgattctacc tctgattttt catcgtcagg gttttcttca tcatattcta
     2101 aacttggtgt ttcttctgtg aaatcttcat cttctggatc atcatcattg taatcaatag
     2161 catcttcatc accatccatg aaagcattga cacgtttctt cttacgtttt ggagcgccat
     2221 cttcatcttc ttcaagagtg atgatttcct cgtcaatttc atcgatagca taccatgaac
     2281 gaagtcccca tttgttttcg ccaagtggaa taaaacttcc gtctgtattc aaatctgaat
     2341 agaaaaatgg caatgactca cggattgctg agtcagattt tccaaggtag gtttgaatat
     2401 catttaccaa atcgctgaag tacatttcgt tatcacgacc acgttgctct aaaatagcgc
     2461 gagcaacttc aatcatcgaa agttcacttt tttcttgccc agcaaatact tctaattcca
     2521 aagttattct ccttaattgt gtaagactat cattttgttt agcaatattc tttatagata
     2581 gaaaatagat aagtcatatt ttcaaatttt cgctatttac tattttacgc tatttttagc
     2641 tattcgtcaa aagaataatc acaaataaaa agactgggaa gcccaatctt ttcaaaattt
     2701 aataagcaat tattttacag tagctgagct agtgataacg tcaaccgctt ttttcattgc
     2761 aatatcatgt ttaagcatat ctgctgaaag tagaccgcgt acttggtcag cttccatatt
     2821 atattctgaa gcaagatctg tgatttcttt ttcgatttct tcatctgttg cttcaaagcc
     2881 ttctgcagct gcaattgctt caataacaag gttagtttta acgcgtttgt cagcatctgc
     2941 ttggtattgc ttatgaaggt cttcttcagt tgtacctgtt aattggaagt acatttctgg
     3001 tgaaattcct tgacgttgca tattacccat gaactcattc atagcacgat gtacttcatc
     3061 atggaccatt tcttctggta attcaacaat ttctgcattg gcaacagcta attcgattgc
     3121 tacaccttca actgcatcat caaatgcaat ttcttttgct gattctagct ctttgcgata
     3181 tttcgctttc aactcatcaa gtgtttcaac ttcatcatca atatcttttg caagttcatc
     3241 atcaagtgct ggaacttctt tagtttttac ttcatgaatt gttgtaacaa atttagcatc
     3301 tttaccagca agatcttctg cttgataatc ttctgggaat gttacattaa cgtctacagt
     3361 ttgaccagct ttagaaccta ctaattgttc ttcaaatcct gggataaatt gacctgaacc
     3421 aagttcaagt gagaagttat ctcctttacc accatcaaat tcaacaccat caactgaacc
     3481 tacaaagtca ataacaactg tatcaccttg tgctgcttca ccatctttaa cagtcaattc
     3541 tgcaaggttg ttacgctcac gttcaacttt agcatcaact tcttcatctg aaacttcttt
     3601 tgaagcatca acttctactg aaaggtcttt gtaatcccca agtttaactt ctggttttgt
     3661 aacaacttca gctgtcaact tccaatcttg acctttttcc attgatacaa catcaatttt
     3721 tggttgagca acaacgtcta aaccaagttc tgcaacagct gcttcgtaag cctttggcaa
     3781 cactaaattc aatgcatttt cgtagagtgc ttcttcacca aatttttggt tgaaaactgt
     3841 acgaggcata tgtcctttac ggaatcctgg aacgttcaaa tcttttttaa ctttgttgaa
     3901 tgcttgatca agagctggct tgatctcatc ttgactaatt gtaaatgtga taataccacg
     3961 gtttgtagct ttattttcaa atgatgtaga cattaagtca tttctcctta aaattattta
     4021 gatacagttt agtttaccat aatttaaaac gttttacaaa gtttttatgc aaaaaagaag
     4081 ctttaaactg cttaacgata agcttttaaa atacttgctc tatggcttt
//
LOCUS       DATRQA010000149         4025 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.149, whole genome shotgun sequence.
ACCESSION   DATRQA010000149 DATRQA010000000
VERSION     DATRQA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.149"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..1676
                     /locus_tag="VBD27_001789"
     CDS             <1..1676
                     /locus_tag="VBD27_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO7025777.1"
                     /translation="HIPFIKQTLKQPELKKHQYILLILFSLFAIISNFTGVEIQSDLS
                     IIPQTLNHIADQSSVANTRVLTIGVSGLIGGPIVGIIVGLLSVFVRYLQGGLAPHIYV
                     ISSLLIGLCSGLSGNYLRKNYNKIRVLDAMVVGFGMEILQMICILIFSVDFNQALRLV
                     SFISMPMILSNTLGLGIFISIISSTQKLEEHAKAFQTHQVLELANLTLPYLRKGLTTE
                     SCQPVAEIIHKHMDVSAVSLTSQSAILAYVGDGADHHLPNTQILTKLAKRAIDTGKVS
                     VATDKSEIECDHRNCPLSSAIVIPLHIHDVIVGTLKLYFSDAQHMTYVDRQLAEGLGN
                     IFSTQLALGQAEEATRLLQDAEMKSLQAQVNPHFLFNALNTIYGLIRMDSEKARKLVQ
                     DFSKVIRANLQRAKQNLIPLHDELEQVNAYLALEEARFPNMVAFNLDNQTNSDDNLMI
                     PPFTLQVLIENSYKHAFKHINKNNQLKVTIARNNDRLHIIVQDNGIGIPKEKLITLGK
                     KTQISKKGSGTAIENLVRRLNIIYDGQASLKFESNDSGTCAIVNIPIKN"
     gene            1688..2422
                     /gene="ltdR"
                     /locus_tag="VBD27_001790"
     CDS             1688..2422
                     /gene="ltdR"
                     /locus_tag="VBD27_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LtdR"
                     /protein_id="HEO7025778.1"
                     /translation="MKVLVVDDEPVARNELIYLLNKYDSNLVIAEAHDMATALAILLR
                     ETFDVALLDIHLRDDSGLQLAEYINKMPKPPLLIFATAYDQYAIQAFEQDARDYLLKP
                     YEFDRLKQAMDRVKGALSTSTIIESVASGPLFKQQYPLTVEDRIYLVSADDILLIEAM
                     QGKLIIQTPDKNYEIDGSLQQWQDKLPSSQFVRVHRSYIVNINAIKTIEPWFNQTLQL
                     HLCNKITVPVSRANVKPLKQMLGIST"
     gene            2578..>4025
                     /locus_tag="VBD27_001791"
     CDS             2578..>4025
                     /locus_tag="VBD27_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEO7025779.1"
                     /translation="MVTFLGGVALLIVGYFTYGRYIEKNFQIDENRQTPAEALRDGYD
                     FVPMPKWKNGMIELLNIAGTGPIFGPILGALYGPVAYIWIVLGCIFAGAVHDYMIGMI
                     SLRNNGAYLPELASRYLGKSMKHVINIFSMLLLILVATVFVVTPANLILSILPAGTLS
                     LPWIIGLIFVYYLISTVLPIDKALGKVYPVFCVILMVSTAAVGFRLLTGGFDMPNLTF
                     ETFKNMHPAGLGIFPALFFTISCGAISGFHATQAPMVSRTTVNEREGRFTFYGMMIAE
                     GVIAMIWAGASMSLFKGQNLYEMIAAGTPSAVVNQVMLMLLGSVIGTIAIIGVIVLPV
                     SSGLSAFRSLRTIVADYIHVKQDTLPKIFAVTIPLYVISFVLTHVDFNLLWRYFNWAN
                     QVTAVIGLLVATRYLILKRRNYWVTFVPAMFMLYAVVVYILSQPIGFNMGLGILTYSL
                     ALVLTGILVGLFWKSGQKQLKTVHPEAFLFND"
BASE COUNT         1254 a          708 c          714 g         1349 t
ORIGIN      
        1 tgcatattcc ctttataaaa caaactttga agcaacccga attaaaaaaa caccagtata
       61 ttctgcttat cttatttagc ttatttgcta ttatttctaa ttttacaggg gtagaaattc
      121 agtcagattt atctatcatt ccacaaactc ttaatcatat agcagatcaa tcatcagttg
      181 ccaacacacg tgttttaacc attggtgtat caggtctaat cggtggcccc atagtcggta
      241 ttatagttgg tctcttatct gtattcgtta ggtacttgca aggaggatta gcccctcata
      301 tttatgtcat ttcttcctta ctaattggtt tatgctctgg gttatcaggc aattatcttc
      361 gtaaaaatta taataaaatt cgtgttttag atgcaatggt tgttggtttc gggatggaaa
      421 ttctacaaat gatctgtatc ctcattttta gtgttgactt taatcaggcg cttcgattag
      481 tatcttttat cagcatgcca atgattttaa gtaatacttt aggtttaggt atatttatat
      541 ccattatttc atctactcaa aaattagaag aacatgccaa agcatttcaa acccatcaag
      601 tacttgaatt agcaaattta acccttcctt accttagaaa gggactgaca acagaatctt
      661 gtcaaccagt agctgaaatt attcataaac atatggatgt gagtgctgtt agcctgacta
      721 gtcaatcagc cattttagcc tatgtaggag atggagctga ccaccatctt cctaatactc
      781 aaatattaac aaagcttgct aaaagagcaa tagatactgg taaagttagt gtcgcaactg
      841 ataaaagtga aatcgaatgc gaccatagga attgcccact tagttcagca attgttattc
      901 cattacacat ccacgatgtt atagtgggta ctttgaaatt atatttttca gacgcacaac
      961 atatgactta cgttgaccgt caattagctg aaggattagg caatatcttt tccactcagt
     1021 tagctttggg acaagctgag gaagcaacaa ggttactaca ggatgcggaa atgaagtctc
     1081 tccaagcgca ggttaatccg cattttcttt ttaatgcact caatactatt tatggtttaa
     1141 ttcgcatgga tagcgaaaaa gcaagaaagc tcgtccaaga ttttagcaag gttattcgtg
     1201 ctaatcttca acgggcaaag caaaatttaa ttcctctcca tgatgaattg gaacaggtta
     1261 acgcctattt agcccttgaa gaagcaagat ttcctaacat ggtggccttt aatttagaca
     1321 accaaacaaa ttcagatgac aacttaatga ttcctccctt tacattgcag gtcttgattg
     1381 aaaactctta taaacacgct tttaagcata tcaataaaaa taaccagcta aaggttacta
     1441 ttgcaagaaa taatgatagg cttcatatca ttgtccagga caatggtatt gggattccaa
     1501 aagaaaaatt aataacgctt ggaaaaaaaa ctcaaatttc aaaaaaaggt tctggtacag
     1561 ctattgagaa tctcgttcgt cgcttgaata ttatttatga tggacaagca agtttaaaat
     1621 ttgaatcaaa tgattcagga acttgtgcta ttgttaacat ccccattaaa aattagaaag
     1681 gtattgtatg aaagttttag tagttgatga tgaaccagtt gcacgtaacg aattaattta
     1741 ccttcttaat aagtatgatt ctaacctcgt tatagcagag gcgcatgata tggctactgc
     1801 attagctatt ttacttagag aaacttttga tgtagcactg ttagatatcc atctcagaga
     1861 tgattctggg ttgcaattag cagagtatat caataaaatg cccaaaccac cattattgat
     1921 attcgcgact gcttatgatc aatatgctat tcaggctttt gagcaggatg cgcgtgatta
     1981 tttgttaaaa ccctatgagt ttgataggtt aaagcaagct atggatagag taaaaggagc
     2041 gctaagtaca tctacaatta tagagagcgt agcttccggt cctctcttca agcaacagta
     2101 tccattgaca gtagaagatc gaatctatct ggtgtcggcg gatgatatcc ttttgattga
     2161 agctatgcaa ggaaaactga ttatacaaac acctgataaa aattatgaaa ttgatggctc
     2221 tctacaacaa tggcaagata aactaccatc atctcaattt gtacgggtac atcgctctta
     2281 cattgtgaat attaatgcta ttaaaacgat tgaaccttgg tttaaccaaa cacttcagtt
     2341 acacctttgt aataaaataa cagttcctgt tagcagagca aatgtaaaac ccctaaaaca
     2401 aatgttaggc atatctacct aactaatgaa aagttattca tcataaaact tagactattc
     2461 atttctaaaa aacaaccatt catcttgaaa ataggaaatt tcaatttttc aatgctatac
     2521 taaagtcatg aaaacgctta ataaaattaa gcaaaaaaat ttttaggagg cagtgatatg
     2581 gtaacatttt taggaggggt agccctttta attgttggtt atttcacgta cggtagatac
     2641 attgaaaaga attttcaaat tgatgaaaat cgccaaacac ctgcagaagc attaagggat
     2701 ggatatgatt ttgttcctat gccaaaatgg aagaatggga tgatcgagtt attgaacatc
     2761 gcaggaactg gacctatctt tggacctatt ttaggagcac tctatggtcc agttgcttat
     2821 atctggatag tcttaggatg tatttttgca ggggctgttc atgattatat gattggaatg
     2881 atttctctta gaaataatgg tgcatatctt cctgaattag ctagtcgcta tcttggaaaa
     2941 tcaatgaagc atgtgatcaa cattttttca atgctattac ttattttagt tgcaaccgta
     3001 tttgtggtaa ctccagctaa tcttatcctg agtattttac cagctgggac actatcttta
     3061 ccttggataa tcggtcttat tttcgtttat taccttattt ctacagtatt acctattgat
     3121 aaagcacttg gtaaagttta tcctgtattc tgcgtcatcc taatggttag tacagcagca
     3181 gttggcttcc gtttattaac tggtggcttt gatatgccaa acttaacttt tgaaaccttt
     3241 aaaaatatgc atccagctgg acttgggata ttccctgctt tattctttac catttcatgt
     3301 ggagccatct ctggtttcca tgctacccaa gcacctatgg tctcaagaac aactgttaat
     3361 gagagagaag ggcgtttcac tttctatggt atgatgattg cagaaggagt aattgcgatg
     3421 atttgggcag gtgcttcaat gagcttgttc aaaggtcaaa atctctatga aatgattgct
     3481 gctggaacac cttctgcagt tgtcaatcaa gtaatgttaa tgttactcgg tagtgtaatt
     3541 ggaactatcg ctatcattgg tgttattgtt ctccctgttt catctggttt atcagctttc
     3601 cgtagcttac gtactatcgt tgctgattat atccatgtca aacaagatac tcttccaaaa
     3661 atctttgctg taactatccc attatatgtt atttcatttg tccttacaca cgttgatttt
     3721 aatctcctat ggcgttactt taactgggca aatcaagtaa ctgctgttat tggtttatta
     3781 gttgctacaa gatatctcat cctaaaaaga cgaaattatt gggtaacatt tgtaccagct
     3841 atgttcatgt tgtatgcagt agttgtttat attttatctc aaccaattgg atttaatatg
     3901 ggcttaggca tcttaactta ttcacttgct ttagtgttaa caggtattct tgttggactt
     3961 ttctggaaat ctggtcaaaa acaattaaaa actgttcatc ctgaagcatt cctgtttaac
     4021 gatca
//
LOCUS       DATRQA010000150         3897 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.150, whole genome shotgun sequence.
ACCESSION   DATRQA010000150 DATRQA010000000
VERSION     DATRQA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.150"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1403)
                     /locus_tag="VBD27_001792"
     CDS             complement(<1..1403)
                     /locus_tag="VBD27_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO7025780.1"
                     /translation="MNKVAIYVRVSTTMQAEEGYSIDEQIDKLTSYCKIKDWTVYDIY
                     KDGGFSGGNIERPAMERLISDAERKKFDTVLVYKLDRLSRSQKDTLFLIEEVFGKNDI
                     SFLSLNESFDTSTAFGKAMIGILSVFAQLEREQIKERMLLGKIGRAKTGKAMMFSKVS
                     FGYTYDKLKDELVVNQAESIIVRKIFEAYLGGLSLNKLRDYLNNNGIYRGDKPWNYQG
                     LRRILSNPVYIGMIRYRGEIYPGNHQAIIDIDDYNKTQEEIKKRQITALEFSNNPRPF
                     RAKYMLSGIAKCGYCGTPLRIILGSKRKDGSRNMRYQCINRFPRNTKGVTVYNDGKKC
                     NSGFYEKADVEEFVINQIRDLQFNINKLDNLFDKHPSIDIKSIERQISSLDNKLKRLN
                     DLYLNDMIDLEDLKKQTQLLRQQKTMLEDELLNNPAITQEKNKKYFKEFLATKDITKL
                     DYETQKNIVNNLINKVFV"
     gene            complement(1551..2093)
                     /locus_tag="VBD27_001793"
     CDS             complement(1551..2093)
                     /locus_tag="VBD27_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO7025781.1"
                     /translation="MNLDTVLQQMKECGAYDTWGTKKEVKALPEILSDDEIIQYATSG
                     FVNGNTVLVVLTQKRILFIDKGMLYGIRSTEIPLDMVNSVSYSKGLILGTIAVMNGAT
                     KIEISNITKDTCPVLTEKIKDCVEQYKYNLYQQQSPQKTTTVSSEDTVAEIRKFKQLY
                     DEGILTEEEFIAKKKIILGI"
     gene            complement(2118..2861)
                     /locus_tag="VBD27_001794"
     CDS             complement(2118..2861)
                     /locus_tag="VBD27_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7025782.1"
                     /translation="MANLSDNIKYFRKQNKLTQKELARKLKIAPTAISAWEVGRNKPL
                     MDNIEQMASIFGIPKSKLLGDEIYKIQETASPELIPSTLQKINATSSQLEHSRQLIVL
                     DTAETQLEQQNTVEDSKNTVVELFSYNYYDHAASAGTGQYLNDVQVETIELPVDYDAD
                     FVIPVYGDSMEPEYHSGDYVFVKLSVELADGDIGVFEYYGDAYIKQLLINNEGAFLHS
                     LNDKYSDIQIDRDSDFRIIGEVIGSYSEK"
     gene            3225..3368
                     /locus_tag="VBD27_001795"
     CDS             3225..3368
                     /locus_tag="VBD27_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025783.1"
                     /translation="MEESILNSKRYTKLVVETEDGKKIAEVTLTEATPADGYVIRLTP
                     NYD"
     gene            complement(3365..3589)
                     /locus_tag="VBD27_001796"
     CDS             complement(3365..3589)
                     /locus_tag="VBD27_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEO7025784.1"
                     /translation="MAKNQHVVPNPNGGWDIKGAGNSRATKHTTTQAEAVEIATGIAK
                     NNSSEVIIHGKNGRIRERNSYGNDPFPPKG"
     gene            3648..3806
                     /locus_tag="VBD27_001797"
     CDS             3648..3806
                     /locus_tag="VBD27_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025785.1"
                     /translation="MNWKKLMLGDLEHTFTSRNGKEKTSIEFEGGVLPALLVLGGITW
                     LIAWLITK"
BASE COUNT         1138 a          721 c          638 g         1400 t
ORIGIN      
        1 acaaaaacct tatttatcaa gttgttcact atatttttct gtgtttcata atctagttta
       61 gtaatatctt ttgtagctag aaactcttta aaatatttct tgtttttctc ttgtgtgata
      121 gctggattat ttagtaattc atcttcaagc atcgtttttt gttgtctaag taattgagtt
      181 tgtttcttca aatcttctag atcaatcata tcatttagat ataaatcgtt gagtcgcttt
      241 aatttattgt ctagtgatga gatttgcctt tcaatacttt taatatcaat tgagggatgc
      301 ttatcaaata aattatctag tttattgata ttaaactgta aatctctaat ctggtttatt
      361 acaaactcct caacatcagc tttctcataa aatccagagt tgcatttttt gccatcgttg
      421 taaacagtaa caccttttgt atttctaggg aaacgattta tacattgata tctcatgttt
      481 cttgagccat ctttacgttt cgagcctaag ataattcgca atggtgttcc gcagtaaccg
      541 cattttgcaa ttccagacaa catatatttt gctctgaatg gtctagggtt gttgctaaac
      601 tcaagagccg ttatttgtct tttctttatt tcttcttgtg ttttattgta atcatcaata
      661 tcgataattg cttgatgatt gccaggataa atttctcccc tatatctaat catgccaatg
      721 tagacaggat tagataatat tctccttaat ccttgatagt tccatggttt atcacctcga
      781 tatattccat tgttattcag ataatccctt aatttattaa gtgagagacc gccaagatac
      841 gcctcgaata ttttcctaac aattatagat tctgcttgat ttactactaa ttcatctttt
      901 aacttgtcat atgtataacc aaacgatact ttactgaaca tcatagcttt accagttttt
      961 gctcttccaa ttttaccgag caacatacgt tcttttattt gttctcgttc gagttgagca
     1021 aatactgata gtatcccaat catagcctta ccgaaagctg ttgaggtatc gaaactctca
     1081 ttaagactta aaaaagagat gtcattttta ccaaatactt cttcgattaa aaacaaggtg
     1141 tctttttgac tacggcttaa tctatcaagt ttatagacta gtacagtatc aaactttttt
     1201 cgttctgcat ctgatatgag tctttccatc gcggggcgct ctatattacc accagaaaat
     1261 cctccatctt tatagatgtc gtaaacagtc cagtctttaa ttttacaata gcttgtgagt
     1321 ttatcaatct gctcatctat agagtacccc tcttctgctt gcatagttgt actcacacgc
     1381 acatagatag caactttatt cattgttttc acctcatttt tctgttaaaa tgggtatggt
     1441 aaaagcccct cccaaaaagc aggtttttac tatactagat tcgcctcacg ctcgcaccga
     1501 ccaaagttga gcgtggggct ttttattttt ttaacggtta actttacacc ttaaattcct
     1561 aaaattattt ttttcttagc tataaattct tcctctgtaa ggatgccttc atcataaagt
     1621 tgtttaaact tacggatttc agcaacggta tcttccgagc ttacagttgt tgttttctga
     1681 ggcgattgct gttggtacag attatatttg tattgctcta cacaatcttt tattttttcg
     1741 gtaagtacag gacaagtatc cttggtaata ttagatattt caatttttgt agctccgttc
     1801 ataacagcta ttgtaccaag aattaaacct ttagagtatg agacgctatt aaccatatca
     1861 agaggaattt ctgtcgatct aattccatat aacattccct tatcgataaa taaaattcgt
     1921 ttttgggtca atacaacaag gacagtatta ccgtttacaa aaccagatgt tgcgtattgg
     1981 ataatttcgt catctgacaa aatttctggc aaagccttta cttccttctt tgttccccaa
     2041 gtgtcgtaag ccccacattc cttcatttgt tgcaaaacag tatctaaatt cataataaaa
     2101 ctccttataa aatattctta tttttccgaa taactaccaa taacttctcc gataatacgg
     2161 aaatcactat ctctatcgat ttggatatca ctatatttat cgtttagact atgtaaaaac
     2221 gctccctcat tgtttataag caactgtttg atataagcgt caccgtaata ctcaaacact
     2281 cctatatcgc catctgcgag ctctacggat agcttaacga atacatagtc cccagagtga
     2341 tactctggtt ccatagaatc gccataaacc ggtatgacaa aatcagcgtc ataatcaact
     2401 ggcaactcaa ttgtttctac ttgcacatca ttcagatact gacctgtacc agctgaagct
     2461 gcgtggtcgt agtagttgta agagaataat tctactactg tattcttact atcttctact
     2521 gtgttttgtt gttctaattg tgtttcagct gtatctaaaa caattagctg tctactgtgt
     2581 tctaattgag aagaagtagc atttattttt tgtagagtag atgggataag ttctggtgat
     2641 gcggtttctt ggattttata tatttcgtca cctaaaagct tcgattttgg tattccgaaa
     2701 atagaagcca tttgttctat attatccatt agaggtttgt ttctacccac ctcccaagct
     2761 gaaatggctg ttggagctat ttttaacttt ctagctagct ctttttgggt tagcttattt
     2821 tgttttctga aatattttat attatccgat aagttagcca ttttttgctc ctttactatt
     2881 atatgtctaa caatattgta cacttcaagt ggaataaatg caatattttt ttagcaaaat
     2941 gcaaaaaaac acttgcaagt acacttcaag tgtagtatac tataatcaag cttaagtaat
     3001 taagcaaaac gaaaggaggt acagctaatg aaatctaggc taaacaaaaa gcctaaacac
     3061 aaagaagtcg agttggaaat tcacattctt tggtttaagc ttaggattca ctactcaata
     3121 gagtggtgac aataccaaga gggctaagaa gccctctccc ctaacggggt aagtttagtt
     3181 tagcacattg gctgtatctc cgcaagaatg aaaggagagt agatgtggaa gaatctatat
     3241 tgaactctaa gcgctacaca aaacttgtcg tagagacaga agatggcaaa aaaatagcag
     3301 aagttacttt gacggaagca acccctgctg atggatacgt gattaggtta acccctaatt
     3361 atgactaacc tttaggtggg aatggatcgt taccatagct gttacgttcg cgaattcgtc
     3421 catttttgcc atggataata acttcactgc tattgttttt agcgatacca gtagcgattt
     3481 cgacagcttc ggcttgggtt gttgtgtgtt tggtcgcacg actgttgcct gcacctttta
     3541 tatcccaacc gccattaggg ttaggtacga catgttgatt tttagccatt agcttatcct
     3601 cctttcgtta aggataagtt gattataaca tttttaggag gtacaaaatg aattggaaaa
     3661 aactaatgct aggcgattta gaacacacgt ttactagtcg taatggcaaa gaaaaaacaa
     3721 gtattgaatt tgaaggcggc gtattgccag cgctattggt gctaggtggt atcacttggc
     3781 tgatcgcttg gcttattaca aaataaaaac tcccatgagg gagtaggagg aaaattatga
     3841 tattaaaaat tattttaatc tcattcttta catcatttat tacgtcatta tttgtta
//
LOCUS       DATRQA010000151         3836 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.151, whole genome shotgun sequence.
ACCESSION   DATRQA010000151 DATRQA010000000
VERSION     DATRQA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.151"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(269..2737)
                     /locus_tag="VBD27_001798"
     CDS             complement(269..2737)
                     /locus_tag="VBD27_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7025786.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKVKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTDDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELVAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YIMPKDGKDYFYARYQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRNQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFAAPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPEEKQQAD
                     KSNENQQPSEASKEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQPE
                     GVQFYNKNGELVTYDIKTLQQINP"
     gene            complement(2750..3670)
                     /gene="lmb"
                     /locus_tag="VBD27_001799"
     CDS             complement(2750..3670)
                     /gene="lmb"
                     /locus_tag="VBD27_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEO7025787.1"
                     /translation="MKKVFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVNVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGHLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
BASE COUNT         1015 a          799 c          709 g         1313 t
ORIGIN      
        1 cccacatacg gctaatctag gactaacccc taatcagaaa gaagaaaatt attttaactc
       61 aataaaataa cacagttttc tgtctactta tttgacatta gtccaagaat cctcctcttt
      121 ttttactatt ctaacatgga taccatttct ttgcaaaaga aaaccgctat tatccaactt
      181 ttatcactac tcgttacata gaatgacttc aaaatcaccg taacttgctt tgacaaagca
      241 gtgctttaac aatgagatct tcttttggtt aagggtttat ttgttgaagt gtcttgatat
      301 cataagttac caattcacca tttttattat aaaattggac accttctggt tggaaaatga
      361 gatacttagg atcgatatta gctttttgtg ctaattgatt gatatgatct tctagggttg
      421 ctctatctag accatagtct ggtaaactgt ctataaagtc atctgattct ttttcttctt
      481 tactggcttc acttggctgt tggttttcat tgctcttatc tgcctgttgc ttttcttcag
      541 gtttatccgt atcagtagca tcaccagcat ttttctttgc caataactct tgagcttgtt
      601 gccactcagc ttgggataga tcggatttat taatggttct caatgaactg ccatctgctc
      661 ttgggatgct aaaggagcca tctttccata caaaagtttc ttttgccaaa acatctcgtg
      721 gatcgaaaat atagccgtct ggtgctgcaa aacgaccttc tgctagggct ttttgaactt
      781 cttcagcaga atggataatt tgccagtttg gcataccagc acgtttatca agaggcgtaa
      841 catttggaat gaccgaacct gactcttcat gccctggctt agaccataca tccggacgaa
      901 cttcggggtg ttgcatcaca tacttgattg ttgcaatctg attgcgcgtc aaccatgaat
      961 acggaacgac atggatatga tcaatatgtg ggataacaaa cgaacttcca gtatcgtaag
     1021 tagcattacc agcatgcatc ggcagacttg acacatctac agcaagtcgt ggctcaatgc
     1081 ctgtatcaac aatgtcataa cggtaatgct tcttatcttt aagcattagt tcttgttcgg
     1141 caaaggcaat ctgagtcaaa tcaagttgat aacgagcata gaaatagtcc ttgccatctt
     1201 ttggcataat atagcccact ttaccatctt ttgttacttt gcgactcact tttttagtgt
     1261 caaagagtgg tttttctttg ccttgttcct gatccaaagc agcaacaagc tcatcagctt
     1321 gaccttttgc tttcacccag ttagcgacct catccaactc atattgttca agttctccaa
     1381 atcctatata gtggaaatga tctccgtgtt tagctgtaac tcctgattta tccactgaat
     1441 gaatggattc tttactaaaa acataagcat cactcgtatc atatggttta ccatctaagc
     1501 cttttccgta agctttgatg cgatgaccaa gaaaggtatg tgtcacttct ttatctgatg
     1561 gttttgagtg atctgaacct gagtcgttgt catcagtttg gccggctaag tatcgatctg
     1621 ctaattccat ttcaagaggt gataactgac ttcttgggat aatatgataa tgatctccat
     1681 gaggcaccac atacccaaaa gcgtttgatt tgatcacttg agtcggttca aaaatcaacc
     1741 catcttcttc cacatgacgg tatttcaaat caagacggtg tagttgatct aaaagttcct
     1801 taaaggtttt tcctttaaac tcatctcttt ggtgtttgtt ttgtgacgca tcatttggcc
     1861 taggaggcgc tggatgataa ccaccgttat ctggctgatg accttgtcca gggttaggcg
     1921 tcacatcagg aattggggct ttcctacgac ctggggctgg tgtcgggcgg taatcagacg
     1981 gtctagcacc tcgaccttgt ttttgactcc agtaggcttg tgcagcagct agctcacttg
     2041 gagacaaatc ttttttagga atataatgat agtgattacc atgaggtact aaataagcat
     2101 ctcctaaatc atcaatgata tctgtcggac taaaaatata gccatcgtct gtagtatagc
     2161 gtccttgtct ttttgcttca ttgactgccg caacttcttc tttactgaga tgggccactt
     2221 gagctaaacc tttttcttta gcttctttag ttcctttggc tacttgctca gcaatttgtt
     2281 gtttggttcg aatgtttttg cgcttactac ctggcttgag gtaaacataa tagttgccat
     2341 tgactttaat aacgtaaccg tctaagattt cattgataac gtctgattgt ttaaaatggt
     2401 aattaggatc cgtcatcaac aactcttcac taataatcgc atcataagga actttcccat
     2461 tgtaaaaatg ataatggtca ccgtgtgagg taacataacc ttggtcagta attttgacta
     2521 cgatctgttc agcagagatg ccttcttcag cactgatttg atccatcgtt ttgtttgttt
     2581 taggggcttt taccttacct ttgctatcat caatataggc aatctgattg tcctttgttg
     2641 ctagacccat atgatgctta ccaagctggt aacttccaat atgagtagct agtaaaatag
     2701 cagcaactga gccgatataa ccatatgttt tcttcactaa taatctcctt tacttcaact
     2761 gttgatagag cacttccaaa tttgctctaa gattttctag atatgtcttg tttccgcttg
     2821 gagcagcttc aagtggactt aatgtcttta ctttagctcc tgttgatttc gcaatagcat
     2881 gagcaatttt ggggttgacg ttgtcttctg caaaaatagt cttgacgttg tattctttaa
     2941 caaagtcttg aatttctttc aattggcgag gagagggctc ttgctctgga gaaatacccg
     3001 agataccaag ttgtttcaag ccgaatcgtt tagccagata agaaaatgcc gtgtgttgcg
     3061 tcacaaatgt ttttgagcgc acctttttaa atttttgagt gtattcttca gttagttgct
     3121 ctgcttcttt tttgaaagcc ttagcctttt tagtgtaact gtctttgtgt ttaggatcca
     3181 aatgtcctag ctctttagcg atattaacag cttcctcacc agctaaaacg ggatccgtcc
     3241 aggtatgtgg gtcataaagt gtcgcagggt caatgccttg tgtgacttcc atatcttcta
     3301 gccctttgac tctatctagt gtcagaggtt ttgacgcttc aaacacatta acctttgatt
     3361 tttttaaatt agggtctaga tcccttgccc aagcttctaa ggtatgtgaa tggtaaacaa
     3421 acaaatccgc gtcataaata gctgccacat catttacaga cggttcaaag gaatgaatgc
     3481 ctgcacctga ttggatcatc ctcacatcat ttaggtctcc agatacttct tttgtcatcg
     3541 catacattgg gtaaaagctg gttacaactg acatgccttg cgtaggctgt ttggggtttg
     3601 ctgacttatc acaccctgct atcattacta aactcacaac catagccatg agaaaaaaaa
     3661 cttttttcat agtacctcct caattataat ttaaccagtt attaaccagt taattttact
     3721 cctttatcaa tgctttgtca atgacaacta ccatctttta tcacatcaac tgaagggata
     3781 agcaaaaggc aattgttgac agctatttgt cctaacttct ttttgatgag gtagca
//
LOCUS       DATRQA010000152         3829 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.152, whole genome shotgun sequence.
ACCESSION   DATRQA010000152 DATRQA010000000
VERSION     DATRQA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.152"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..337)
                     /locus_tag="VBD27_001800"
     CDS             complement(<1..337)
                     /locus_tag="VBD27_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEO7025788.1"
                     /translation="MSTLTIIIATLTALEHFYIMYLETLATQSNMTGKIFSMSKEELS
                     YLPVIKLFKNQGVYNGLIGLFLLYGLYISQNQEIVAIFLINVLLVAVYGALTVDKKIL
                     LKQGGLPILA"
     gene            complement(404..2722)
                     /locus_tag="VBD27_001801"
     CDS             complement(404..2722)
                     /locus_tag="VBD27_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEO7025789.1"
                     /translation="MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTK
                     NLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKNDSLDFHFTTAKRAEKELEKGHYYMV
                     ITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEVAESITGT
                     YTESVFKNMGSMKTGINKAADGSQELLNGSNKLQNGSQTLTSNLDVLASSSQTFSGGA
                     NKLNSGINLYTDGVGTLSNGLETLSDGVTAYTTGVHKLSEGSQKLDDKSQALVEGSEK
                     LTVGLQQLSQATQLKPEQERTLQNLSDGLKNLNQIITNLQSTATTDSDTNSKLFNFLS
                     TIESSTTALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTSAETIAAN
                     ISSNIENMKTVLSEASSSAPSNNNSQNFQTLSGTANNLVLKAISDLDKIQKLPTATKQ
                     LYQGSQTLTKGITDYTNAVGQLRKGAETLDSKSNQLISGTQKASQGAQTLDSKSDQLR
                     DGAGQLASGSDRIADGSNKLAGGGHQLTDGLTELSGGVSQLSSSLGKAGDQLSMVSVN
                     KDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAKALSGKEPA
                     NRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTA
                     LVGWDNRYGSFLSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETIS
                     LTGDVNHQWRMLVIFLVSSMILALLIYRKQED"
     gene            2861..3400
                     /locus_tag="VBD27_001802"
     CDS             2861..3400
                     /locus_tag="VBD27_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEO7025790.1"
                     /translation="MVNDTRREKTKRAIEAAMITLLKDQSFDEISTINLTKTAGISRS
                     SFYTHYKDKYEMIDQYQQSLFNKVEYIFDRNQFKKEDALLEIFQFLDRESLFAALLTQ
                     NGTKEIQTYILNKLQLMLSKELPVVNPDATKSDINRLYYSVYLSHAIFGVYQMWITRG
                     KKESPQQITQVLLSLLPQT"
     gene            complement(3486..3782)
                     /locus_tag="VBD27_001803"
     CDS             complement(3486..3782)
                     /locus_tag="VBD27_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEO7025791.1"
                     /translation="MNLNDRLKIEEMEEKYDSFKPRINALVEAIDDFQKHYEDYVKLR
                     EFYGSEDWFRLSEQTENNLKCGVLSEDQLFDFIGEHNELVGQFLDMSSQMYRHL"
BASE COUNT         1220 a          715 c          579 g         1315 t
ORIGIN      
        1 gagctaatat aggtaaacca ccctgtttta ataagatttt tttatcaact gtcaaagcac
       61 cataaacagc aactagcaac acattgatta aaaaaatagc tacaatttct tgattctgtg
      121 aaatatataa cccataaagg aggaatagac caatcaagcc gttgtataca ccttgattct
      181 taaaaagttt aataacgggt aaatatgaca actcttcttt agacatacta aaaatcttcc
      241 cagtcatatt tgactgggtg gctaacgtct ccaaatacat aatataaaaa tgttccaaag
      301 cagttaatgt tgcaataatt attgttaaag tagacatatc ttctcctcct aaatactttg
      361 acatagcata tcatactgtt agtctatcac tagatacttt ctattaatct tcttgtttac
      421 gataaataag aagagcaagt atcatcgatg atactaaaaa gattactagc attctccatt
      481 gatggttaac gtctcctgtc aacgagatgg tctcccttaa tcctgaaaca gagtaagtca
      541 tcggtaaaaa tggttgaatt gtttgaaaga acttaggact caattctatt gggtaagttc
      601 ctgcgcttga accaagctgg aataataata ttaataacga caagaaggaa ccatacctat
      661 tatcccatcc cactaaagca gttactaatg ccatcaaagt ccatgctgtc aataggataa
      721 taaagtaggt ttttccaggg taatctggtt taagacctat gaattgaact gcaaaaaata
      781 agatagttgc tgctagagtt gcaatgaatc cattgattaa taatttattt ttagcccaac
      841 taaaacgatt agctggctct ttgccagata aagctttcgc aaaaataaca ttggctgata
      901 aagctaccac catcaacgca actgaaatca tgtatggtgc cattccaaca ccattcgtat
      961 caacgctatc ataatcttca tgcttgatag tgactgggct agatacagca ttggcattat
     1021 ccttgtttac agaaaccatg gacaactggt ccccagcttt acccaaagaa cttgataatt
     1081 gagaaacacc acctgacaac tctgtcaacc catctgtcaa ctgatgtcct cccccagcaa
     1141 gcttattaga accatctgca attctatcag aaccactagc taattgcccc gctccatctc
     1201 tcaattgatc cgatttacta tctaaagttt gagctccctg acttgctttt tgtgttcctg
     1261 aaatcaattg attggattta ctatctaaag tttctgcacc tttgcgtaat tgccctacag
     1321 cattcgtata atccgtgatt ccttttgtca aggtttgact accttgatat aactgcttag
     1381 tagccgttgg caatttttga attttatcta aatctgagat agcttttaat actaaattgt
     1441 tagctgttcc tgacaaagtt tgaaaatttt gtgagttatt attagaaggg gctgaactag
     1501 atgcttcact aagcacagtt ttcatatttt ctatatttga agaaatattt gctgcaattg
     1561 tctcagctga agtaggtgtt ccagtaaccg ctgaggtaat ttgagattgt tgttcagggg
     1621 ttaaactttt aaaagctgac gttgattgta ccgcagtcat ttgcttttgt ttatcagcag
     1681 cagccgtatt catcaaagcc gttgtacttg attcaatagt tgataaaaaa ttaaataact
     1741 tactattagt atcgctatca gtagttgctg tcgattgtaa atttgtaata atctgattta
     1801 gattctttaa tccatctgaa aggttctgca aagtacgttc ttgctctggt tttagttgag
     1861 ttgcttgtga caattgttgt aaaccaaccg tcaacttttc tgatccttcc accaatgctt
     1921 gagacttatc atctaatttt tgactacctt cactcaactt atgcacacct gttgtgtaag
     1981 cagtaacacc atctgataat gtttctaacc cgttagatag ggttcccaca ccatctgtat
     2041 aaaggtttat tccactattt agtttattag ctcctccaga aaaagtttga ctagagcttg
     2101 ctaaaacatc taaattactt gtcaaggttt ggctcccatt ctgtagctta ttagaaccat
     2161 ttaataattc ttgactccca tcagcagctt tattgatacc tgttttcata gaacccatat
     2221 ttttaaaaac tgattcagta taagtccctg taatagattc tgctacctca tcttttagtt
     2281 tattagctgc tgtctcactc atttttgaag ctacaaaact acgcccctta gtagttttat
     2341 aagtaatatt taacctttct ggtttttctg tcatgagagt tgtcgctttt ctagaaagat
     2401 ctttaggaaa ggtaataacc atataatagt gacccttttc taattctttt tctgctcttt
     2461 tagctgttgt aaaatggaaa tctaagctat catttttaga caaattgtcc tccatatctt
     2521 tgccaattga aatagttttt ccatttaatt tagccgattt atcttggtta acgactgcca
     2581 caggtaaatt tttagtattc ccataaggat cccacattga gcttagaaag ataacattat
     2641 atagcgttgg aatcagtgca accccagcca tcgtaatcca taattttggg ctttttatga
     2701 ttgcttttaa ttcgtctaac attttttctc ctattttgga catattgttc atttttttga
     2761 taaaatatat tatacagtga tgatagataa tttcaagata aaaatctcgt ttttggacac
     2821 tgtgtttaaa aatgtacaaa aatattaagg ggtaattaat ctggtaaacg atacgaggag
     2881 agaaaaaaca aaaagagcta ttgaagcagc tatgattacg ttattaaaag atcagagctt
     2941 cgatgaaatt tcaacgatta atctaactaa aacagctgga atcagtcgtt ctagttttta
     3001 tactcattat aaagataaat atgaaatgat tgatcaatac caacagtcac tcttcaataa
     3061 agtcgaatat atttttgatc gcaatcaatt taaaaaagaa gatgccttgc tcgaaatttt
     3121 tcaattctta gatagagaat cattatttgc tgctctctta acccaaaatg gaacaaaaga
     3181 aattcagacc tacatcctta ataaattaca attaatgctt tctaaagaat tacctgtggt
     3241 caatccagat gcaacaaagt ctgatatcaa ccgtttgtat tatagtgtct atctcagtca
     3301 tgctattttt ggagtctatc aaatgtggat aacacgagga aaaaaagaaa gccctcaaca
     3361 aattacacaa gtgctactca gtttattacc tcaaacttaa aaccagcctt ccttataggc
     3421 tggtcatttt tagttaggtt tattttatcc tcattatagg ataacaacac taataaggac
     3481 tgacattata aatgacgata catttgagaa gacatatcta aaaattggcc tacaagctcg
     3541 ttatgttctc ctataaaatc aaagagctga tcctcactca gtacaccgca tttgagatta
     3601 ttttctgttt gttctgatag tctaaaccag tcttcgctac cataaaattc tcttaatttc
     3661 acgtaatctt catagtgctt ttgaaaatca tctatagctt ctactaatgc attgattcta
     3721 ggtttgaagc tatcatactt ctcttccatt tcttctattt ttaatctatc atttagattc
     3781 ataataaaat ctccttagtt atataagatt cgacgagata agttatcat
//
LOCUS       DATRQA010000153         3824 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.153, whole genome shotgun sequence.
ACCESSION   DATRQA010000153 DATRQA010000000
VERSION     DATRQA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.153"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(85..600)
                     /gene="lacB"
                     /locus_tag="VBD27_001804"
     CDS             complement(85..600)
                     /gene="lacB"
                     /locus_tag="VBD27_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEO7025792.1"
                     /translation="MKIAVGCDHIVTYDKIAVVDYLKTKGYEVIDCGTYDNIRTHYPI
                     YGKKVGEAVASGKADLGVCICGTGVGINNAVNKVPGIRSALVRDLTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIRAKYKPTKENKVLIEKIAEVETHNAHQEENDFFTEF
                     LDKWNRGEYHD"
     gene            complement(621..1045)
                     /gene="lacA"
                     /locus_tag="VBD27_001805"
                     /pseudo
     CDS             complement(621..1045)
                     /gene="lacA"
                     /locus_tag="VBD27_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
     gene            complement(1295..3745)
                     /locus_tag="VBD27_001806"
     CDS             complement(1295..3745)
                     /locus_tag="VBD27_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEO7025793.1"
                     /translation="MTLTFGKPSITIRRLGVITSTYMMLGFILLCGLLHPSTIYSEEI
                     KSDINSFISIDDEKTTAPIFDSTLDKLTNSTEERQLELDSDTSDGLIVDDQKDKDMVV
                     VLGDSDSQEKLSPPLKSEIATEENRKQANLAELNATQPNNRTTYIIPESSHSIAEQQR
                     FLIESKGSSVALLNSDEFRKTAGEDRGFERDKLRSLDIIPKGDLSTSNVIGNTDIASQ
                     ISLGFKKNAMQEHHLTKTFSQKDGKLSSVIEGMLAIGKEKVEKEIKYSGNLWQKLKAK
                     AHCLVCCVDNLNFEDIKSYFQYYCHLNHQLKLPKGAILSAKTEVYRGGDFGRKNKDNV
                     FGYRIPSLLKTQKGTLLAGADERIEQACDWGNIGMVIRRSEDDGVTWGKRETIVNLRN
                     NPRVPLVTSGDYSGSPINMDMALVQDTSSKTKRIFSIYDMFPEGRGVISIANTPEKEY
                     TQIGGQSYLNLYNNGKKSKVFTIRDKGIVYNFKGKKTDYHVITETTKSDHSNLGDIYK
                     GKQLLGNIYFTKHKTSPFRLAKSSYVWMSYSDDDGRTWSSPRDITASLRQKGMKFLGI
                     GPGKGIVLKWGPHAGRIIIPAYSTNWKSHLRGSQSSRLIYSDDHGKTWHTGKAVNDNR
                     ILSNGEKIHSLTMDNKKEQNTESVPVQLKNGDIKLFMRNLTGNLEVATSKDGGETWQN
                     HVKRYKEIHDAYVQLSAIRFEHDKKEYILLVNANGPGKKCQDGYARLAQVNRNGSFKW
                     LYHHHIQDGSFAYNSVQQLNNDQFGVLYEHREKHQNSFTLNYKVFNWSFLSQNTEKQG
                     TLWEKMAANWHVLFKFYL"
BASE COUNT         1129 a          787 c          550 g         1358 t
ORIGIN      
        1 acagtatcca tgttaaagtt ttctaagcag tacgaaatat caatagaagg gttcaaagtt
       61 actgttaaaa tcatagatat ctccttagtc gtgatattca ccacgattcc atttatctaa
      121 gaactctgtg aaaaagtcat tctcttcttg atgggcatta tgagtctcaa cctcggctat
      181 cttttcaatt aatactttgt tttcttttgt tggtttatat ttagctctta taaacgcctc
      241 gatgatatct gtcattaaca gcccaccagt aattttccca ccaaatccga taacgttagc
      301 atttaattct tctttagcat agatagctga tgtcaaatca cgtaccagag ctgagcgaat
      361 tcctggaact ttattaacag cattattaat accaactcca gttccacaaa tacatacacc
      421 taagtcggct tttccactag cgacagcttc tcctactttc ttaccataaa taggatagtg
      481 agtacggatg ttatcataag ttccacaatc aataacctca tacccttttg tttttaaata
      541 atccacaact gcaattttgt cataagttac aatatgatca caaccgaccg caattttcat
      601 ctgctttacc tctcttacgc ttagcacatt ttattcaaca tatcgacccg aatttgatga
      661 cgacctgcat cgtaagtccc atcaacaaaa ccctctacta tatgtttagc aaccccggac
      721 caacaatctc tgaacctaaa gtaatcatcc tagcgttatt atgagctcta gtcatataag
      781 ctgagcgttc atctgaaact tctgctgcaa tcatcccctt aaccttagtg gctaccataa
      841 aaggaccagc accgtaagca tcaaccatta ttcctaagtt atcttctttt tgattcactt
      901 tagcaacgat tgctaaggta ttatcaacga aatctttgct agtatccgtt aaatcaataa
      961 tttccttacc taaacgtgta aggtgttgtc taatcacttc cttaagttca acaccatgcg
     1021 catccgcccc aataataatt gtcatttctt tctccttatt tcacttttaa tacaattgta
     1081 tgtagttgga agatattgtt tgttttttgt ttgtaatatc atcatatatc ttatttttgt
     1141 ttttgtcaat tttttgtttt tgtttttgtt tgaaataaac aaaaaaaact ccgtgataac
     1201 tatggcaact agaattcact actagctagc tatcacgaag tttgatactt ctcattattt
     1261 ttatttgtag gaacggttat tccgcaagcc ataatcataa ataaaattta aacaaaacat
     1321 gccaatttgc tgccattttc tcccataaag tgccttgctt ctctgtattt tgactaagaa
     1381 aactccaatt aaaaactttg taatttaaag taaaactatt ttgatgtttt tctctatgtt
     1441 cataaaggac accaaattga tcattattaa gttgttgaac agagttgtaa gcaaacgaac
     1501 catcttgaat gtgatggtga tataaccact taaaactacc atttcgatta acttgcgcta
     1561 gacgtgcata tccatcttgg cacttcttcc ctggcccatt agcattcact aataaaatat
     1621 actctttttt gtcatgctca aagcgaatag ctgatagttg gacgtaagca tcatgaattt
     1681 ccttatatcg tttaacatgg ttttgccaag tctccccgcc gtctttactt gtggctactt
     1741 ctaggttacc agttagattc ctcataaata acttaatgtc cccatttttc aattgaacgg
     1801 gtacggattc tgtattttgt tcttttttat tatccattgt taaggagtga attttttcac
     1861 cattagaaag tatacggtta tcattgactg cttttccagt atgccacgtt tttccatggt
     1921 cgtctgaata aattaggcgt gaagattgtg aacctcttag atgagatttc caattcgtag
     1981 aataggcagg aataataata cgaccagcgt gtggccccca ttttaaaact ataccttttc
     2041 caggtcctat tcccaaaaat ttcatgcctt tctgacgaag acttgctgtt atatctctag
     2101 gtgatgacca tgtcctacca tcatcatcgc tatatgacat ccacacatag cttgattttg
     2161 ctaaacgaaa tggtgacgtt ttatgttttg taaaatatat atttccaagt agctgttttc
     2221 ccttataaat atcccctaga tttgaatggt cacttttagt agtttctgtt ataacatgat
     2281 aatcagtctt tttcccttta aaattatata caataccttt gtcacggata gtaaaaacct
     2341 tcgatttctt tccattatta tataaattaa gataagactg tcctccgatt tgggtatatt
     2401 ctttttcagg tgtgttagca atactaataa cgcctcttcc ttctggaaac atatcatata
     2461 ttgaaaaaat acgtttcgtc ttggagctag tatcttgaac taatgccata tccatattaa
     2521 taggtgagcc actatagtca ccactagtaa ctagcggaac tctagggtta ttacggagat
     2581 tgacaatagt ttctcttttt ccccaagtga caccatcatc ctcactacgg cgaataacca
     2641 ttcctatgtt tccccaatca caagcttgct caattctttc atcagctccc gcaagtaaag
     2701 ttcctttttg ggttttcaat aatgagggga tacggtaacc aaacacatta tctttatttt
     2761 ttctcccaaa atctcctccc ctatatactt ctgttttagc agaaagtata gcacctttag
     2821 gtaatttgag ctgatggttt agatgacaat aatattgaaa ataagatttt atatcttcaa
     2881 aattcaaatt atcaacacag caaacaaggc agtgtgcctt agcttttaat ttttgccata
     2941 aattaccact atattttatt tctttctcta ctttctcttt gccaatagca agcatcccct
     3001 ctataacaga cgataacttt ccatcctttt gagagaatgt tttagtaaga tggtgttcct
     3061 gcatcgcatt ctttttaaag cccaacgata tctgactagc aatgtccgta ttacctatga
     3121 cattacttgt cgataaatct cccttaggaa tgatatccaa agacctcaac ttatcccttt
     3181 caaaacctct atcctctccc gctgtctttc taaattcatc gctattaagt aatgcaaccg
     3241 aagaaccctt tgattctatc aggaatctct gttgttctgc aatggaatga ctgctttcgg
     3301 gtataatata ggtagttcta ttattaggtt gagttgcatt tagttctgct aaatttgcct
     3361 gtttacgatt ttcttctgtc gctatttccg attttagagg aggtgacaat ttttcttgac
     3421 tgtcagaatc acctaagact actaccatat ctttatcttt ttgatcatct actattaaac
     3481 cgtccgatgt atcagaatct aattctaact gcctctcctc tgttgaatta gtaagcttat
     3541 ctaaagtaga gtcaaatatg ggagcagtag ttttttcatc atctattgat ataaaagagt
     3601 ttatatcaga ttttatttcc tcactataaa ttgtactagg atgcaaaagt ccacataata
     3661 agataaaacc tagcatcata tatgtactag ttatcactcc tagacggcgt atcgttatag
     3721 aaggtttccc aaaggttaat gtcatagaaa ctccaagtct taaaatattg gttgcataca
     3781 tcaactctag aaaaatggga gttcagtaat gtttccaaaa agga
//
LOCUS       DATRQA010000154         3808 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.154, whole genome shotgun sequence.
ACCESSION   DATRQA010000154 DATRQA010000000
VERSION     DATRQA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.154"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            1..1020
                     /locus_tag="VBD27_001807"
                     /pseudo
     CDS             1..1020
                     /locus_tag="VBD27_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767962.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
     gene            complement(1231..2043)
                     /locus_tag="VBD27_001808"
     CDS             complement(1231..2043)
                     /locus_tag="VBD27_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO7025794.1"
                     /translation="MLLDMLSYDFMQRALLAVVAISIFAPILGIFLILRRQSLMSDTL
                     SHVSLAGVALGVVLGISPTWSTIFVVTLAAVVLEYLRTVYKHYMEISTAILMSMGLAI
                     SLIVMSKAHNVGNVSLEQYLFGSIITIGKEQVIALFVIALITFILTILFIRPMYILMF
                     DEDTAFVDGLPVRTMSILFNVVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGRNFK
                     TVIFLGMLIGFVGMVAGIFLSYYWETPASATITMIFIGIFLLVSLVGLLRKR"
     gene            complement(2033..2743)
                     /locus_tag="VBD27_001809"
     CDS             complement(2033..2743)
                     /locus_tag="VBD27_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO7025795.1"
                     /translation="MRYITVSGLTFQYDSDPVLEGVNYHLDSGEFVTLTGENGAAKST
                     LIKATLGILTPKVGTVNISKENKEGKKLRIAYLPQQIASFNAGFPSSVYEFVKSGRYP
                     RNGWFRRLTKHDEEHIRVSLEAVGMWDNRHKKIGSLSGGQKQRAVIARMFASDPDIFV
                     LDEPTTGMDAGTTEKFYELMHHNAHKHGKSVLMITHDPDEVKGYADRNIHLVRNQSLP
                     WRCFNVHTNEMEVESDAT"
     gene            complement(2746..3189)
                     /locus_tag="VBD27_001810"
     CDS             complement(2746..3189)
                     /locus_tag="VBD27_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEO7025796.1"
                     /translation="MTVLEQKLDHLVSQILLKAENQHELLFGTCQSDVKLTNTQEHIL
                     MLLSQEQLTNSDLAKKLNISQAAVTKAVKSLISQDMLKANKDSKDARITYFELSELAK
                     PIADEHTHHHDNTLGVYGRLVNHFSKDEKVVLERFLDLFSRELEG"
     gene            complement(3276..>3808)
                     /gene="ipk"
                     /locus_tag="VBD27_001811"
     CDS             complement(3276..>3808)
                     /gene="ipk"
                     /locus_tag="VBD27_001811"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEO7025797.1"
                     /translation="ATIRALNRLWNLQMGHDEMVAIGFKIGSDVPYCLGGGCSLVLGK
                     GEIVKPLPTLRPCWIVLVKPDFGISTKSIFRDIDCKSISRVDIDLLKSAILSSDYQLM
                     VKSMGNSLEDITITKNPVISTIKERMLNSGADVALMTGSGPTVFSMCSTEKKADRVFN
                     SMKGFCKEVYKVRLLR"
BASE COUNT         1397 a          752 c          575 g         1084 t
ORIGIN      
        1 taaacaatta tacaaatcac tcccactatt aactgtcgta ctatgcgttg gggcattaca
       61 acagaatgtt gaagctaagg ctaaacatta taagacgaca tcccatgttg agacacagta
      121 tgtaagtact tcttcaaaaa aatcttaccg tttactcata ataagcaaat caaggtaggt
      181 tcattagata acttagaaag agcaacatat tctcacatac aattaaggga tgcagatgag
      241 cctaagataa aaagagaaag actgacgtat aatccaaccg gttggcacaa ctacaaattt
      301 actactgaaa agggtaaaac aacttggctt atagaccgag gtcacctcgt tggttatcaa
      361 ttttcaggaa tgaacaatgt tcctgagaac ctagttacta tgaccaaata tctaaataca
      421 ggatttagcg aaaataatcc tgatggtatg ttatactacg aaaatagatt agatagttgg
      481 ttggcaaatc ataaaaattt ctagcttgat tacaaagtga ctccaatata cgaaggaaat
      541 aatttagtac ctagccgagt tgaacttcag tatgttggaa ttgataaaca aggaaaactt
      601 ctagaaatca aattaggcgg cggcaaagaa caagttgatg aatacggcgt cacaactgtt
      661 acattagaaa atacgtctcc actcgcaaaa attgattaca aaactggtat gctaatcaaa
      721 gaagatggga aacaggcaga agaaggtgaa gatccaaata gcgatgctga tgaaaacgaa
      781 gcagctattg aaggtgcatc tgatatagaa gaaaatgcta acactaatac ttctgaatca
      841 gatacaaata atgtagctcc caaaaataga atcgtctacg ttgcaaataa gggtcgttca
      901 aatacttatt ggtatagttt agaaaacata aaaaatgcaa ataccgctaa tatcgttcaa
      961 atgactgaac aagaagcgtt aaatcaacac aaatatcaca gcactactga agcacaataa
     1021 ttacaattta ctgccggcaa aattgccggt agtatttttt atcctaaaac gcaaaaatag
     1081 attcacattg ccccttctat caacttaagc cagaaaactt agtagatacc tcatcgagat
     1141 aatttctcaa ctgatccctc ataaaatata ttcgaaaaaa acagggagat aaataaaaaa
     1201 tctatcctag aataagatag atttttttat ttaacgtttg cgaagcaagc ctactaagct
     1261 aactaatagg aaaatgccaa taaaaatcat cgttattgtt gcactagcag gtgtctccca
     1321 ataataagaa aggaaaatac cagctaccat tcccacaaat ccaattaaca tcccaagaaa
     1381 aataacagtt ttaaaatttc gacctaaccg catagcaata ctagctggta aaaccataat
     1441 agttgacacc aatagagcac ctgcggcagg aattgttaac gcaatagcaa tacctgtaac
     1501 aacattgaac aaaatagaca ttgtacgcac aggcaatcca tctacaaaag ctgtatcctc
     1561 atcaaacatt aatatataca ttggtctaat aaataggatg gttaaaataa aagtgattaa
     1621 tgcaataaca aacaaagcaa tcacttgttc cttcccaata gtgataatag aaccaaataa
     1681 atactgttca agactgacat taccaacatt atgagcttta ctcataacga ttaaagatat
     1741 agccaacccc atcgacatca ggatagcagt tgatatttcc atataatgtt tgtatacagt
     1801 acgcaaatac tctaatacaa ctgccgccaa agtaacaacg aaaatagttg accacgtagg
     1861 cgagatacct aaaacaacgc ctaatgcaac accggctaag gaaacgtggc tcaaagtatc
     1921 actcatcaaa ctctgacgac gtaaaattag gaagatgcct aaaattggag caaaaatact
     1981 aatagccaca actgctaaga gcgctcgttg cataaaatca taggaaagca tatcaagtag
     2041 catctgattc aacctccatt tcgtttgtat ggacattgaa acaacgccat ggtaaagatt
     2101 ggttcctaac aagatgaata tttcggtcag catacccctt tacttcatca ggatcgtgag
     2161 taatcatcaa aacagactta ccatgcttat gagcattatg atgcattaac tcataaaatt
     2221 tctcagtagt ccctgcgtcc ataccagttg ttggctcatc caatacaaaa atatctggat
     2281 cagaagcaaa catcctagca atgacagccc tctgcttttg accgccagat aaacttccta
     2341 tctttttatg gcgattatcc cacataccaa cagcttctaa actaacccta atatgttctt
     2401 catcatgttt agtcagtctt ctaaaccaac catttctagg gtatcgtcca gatttcacaa
     2461 attcataaac tgacgatgga aagccagcgt taaaacttgc aatctgttga ggtaaatagg
     2521 caatacgtaa tttttttcct tctttgtttt ctttagagat attaactgta cccactttgg
     2581 gcgttaaaat acctaaagta gcctttatta aagttgattt agcagcccca ttttcacctg
     2641 ttaaggtaac aaactcccca ctgtctaaat ggtaattaac cccttccagc acaggatcgc
     2701 tatcatactg aaaagtaaga ccactaactg taatatatcg catgattacc cttctaattc
     2761 tctagagaaa agatcaagaa aacgttctaa aacgaccttt tcgtcctttg aaaaatgatt
     2821 tactaatctt ccgtaaaccc ctaacgtatt gtcatgatga tgtgtgtgtt catctgcaat
     2881 gggtttagca agttcagata actcaaaata agtaatacga gcatctttag aatctttatt
     2941 cgctttcaac atatcctgag aaattaaact ttttactgct ttagttacag ctgcctgact
     3001 aatatttaac ttcttagcta aatcagaatt tgtcaactgc tcttgtgata aaagcatcag
     3061 aatgtgttct tgcgtattag tcaattttac atcactttga caagtaccaa acaataattc
     3121 atgttgattt tctgccttaa gcaagatttg actcactaaa tggtctaatt tttgttctaa
     3181 aactgtcata tatacctctt tttttgttaa ccagtaaatt atatcacgaa gatatagaag
     3241 aatcaatcat agataggtga agaagataaa accttttatc tcaacaacct aactttataa
     3301 acttctttgc aaaaaccttt catactatta aaaacacgat cagctttttt ctctgtagaa
     3361 cacattgaaa aaacagttgg tccacttcct gtcattaatg caacatcggc tccagaattt
     3421 aacatacgtt cttttattgt acttataact ggatttttag taattgtaat atcctcgagt
     3481 gaatttccca tagatttgac cattaactga taatctgatg acaaaatagc agactttaat
     3541 aaatcaatat caactctgct tatagactta caatcaatat ctctaaaaat ggatttagtt
     3601 gaaataccaa aatccggctt aaccagaact atccaacatg gtctcaatgt cggtaagggt
     3661 ttaacaattt cacctttacc taatactaac gaacatcccc caccaaggca ataaggaaca
     3721 tcactaccaa ttttaaaacc aatagcaacc atttcgtcat ggcccatttg aagattccat
     3781 aatcgattaa gagctcttat tgtagcag
//
LOCUS       DATRQA010000155         3723 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.155, whole genome shotgun sequence.
ACCESSION   DATRQA010000155 DATRQA010000000
VERSION     DATRQA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.155"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(328..1923)
                     /gene="cls"
                     /locus_tag="VBD27_001812"
     CDS             complement(328..1923)
                     /gene="cls"
                     /locus_tag="VBD27_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO7025798.1"
                     /translation="MIWRGEKLISNKVKIVRLLNKSKKSLLRGIFSRTTVIAILLILQ
                     LLFLLASYSWLEQYRVWLATVEHILTIGAVLYLVNSEMDALSRVTWLILVMIAPLLGA
                     MFLMYTKFDWGYRGLKQRLETLIDESQIYLEDDPETLNQLKSSTSTTYHLVQYFEKAH
                     GNFPVYRNTDVTFLPTGEAFFEKMKEELLKAKKYIFLEFFIIDEGIMWGEILSILEQK
                     VEEGVEVRILYDGMIEITKLSFDYTKRLEKIGIKAKAFSPISPFISTYYNYRDHRKIV
                     VIDGVVGMTGGVNLADEYINHIELFGHWKDSGIMLKGKAVDSFLLLFLQMWSITEEKM
                     LVAPYLGVHDDLVENEGYVIPYGDSPLDTDKVGENVYIDILNHAREYVYIMTPYLILD
                     SELEHAIQFAAERGVDVRIIMPGIPDKPIPYALAKTYYQALIKSGVKIYEYTPGFVHS
                     KIFLSDNTKAVVGTINLDYRSLYHHFECAVYLYKVDAIQDIYRDYMDTLNKSRLVSLK
                     DINNIPKFQKVIGIVTKTIAPLL"
     gene            complement(2042..3712)
                     /locus_tag="VBD27_001813"
     CDS             complement(2042..3712)
                     /locus_tag="VBD27_001813"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEO7025799.1"
                     /translation="MKTDIEIAQSVALKPIAEIVEQVGIGFDDIELYGKYKAKLSFDK
                     IEAVRSQKVGKLILVTAINPTPAGEGKSTMSIGLADALNKIGKKTMIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELDIDQRR
                     VIWKRVVDLNDRALRQVIVGLGSPVNGIPREDGFDITVASEIMAILCLATDLSDLKKR
                     LSNIVVAYSRDRKPIYVKDLKIEGALTLILKDAIKPNLVQTIYGTPALVHGGPFANIA
                     HGCNSVLATSTALRLADYVVTEAGFGADLGAEKFLDIKTPNLPTSPDAIVIVATLRAL
                     KMHGGVSKEDLSQENVEAVKRGFTNLERHVNNMRQYGVPVVVAINQFTADTESEIATL
                     KTLCSNIDVAVELASVWEDGADGGLELAQTVANVIETQSSNYKRLYNDEDTIEEKIKK
                     IVTKIYGGNKVHFGPKAQIQLKEFSDNGWDKMPICMAKTQYSFSDNPNLFGAPTDFDI
                     TVREFVPKTGAGFIVALTGDVLTMPGLPKKPAALNMDVLEDGTAVGLF"
BASE COUNT         1195 a          756 c          555 g         1217 t
ORIGIN      
        1 aagaaattgt gacttattta tgcaaaaaaa agaaagaacc aaactggttc tttctttttt
       61 gcttttcagc tacttaccac agtttgtaat gaattacaag tagtagtaag ctaagggcac
      121 cttttcaagt gtgataagaa ggttcacaca acttatcaag atccgctcct gcgtttataa
      181 tctccctgcg caatagtggt aaaaccacat atcgactcat cgcataacag tagtttaaca
      241 taatttagtt aaaatgcaac ctttactttt taaatataac tagattcttc agtttacttt
      301 taatattaaa ttaaaaataa attatttcta aagcagggga gcaatagttt ttgtcactat
      361 gccaattact ttttggaatt ttggaatatt atttatatct ttcaagctaa ctaaccttga
      421 tttatttaac gtatccatat aatctcgata aatgtcttgg attgcatcta ccttataaag
      481 ataaacagca cattcaaaat ggtgatacag gctacggtaa tcaagattta tcgtacctac
      541 aacagccttt gtattatcac taaggaatat ttttgaatga acaaatccag gggtgtattc
      601 atatatttta acacctgatt ttattaatgc ttgatagtaa gttttagcaa gagcataagg
      661 gattggctta tctgggatac ccggcataat aatacgtaca tctacaccac gttccgcagc
      721 aaactgaatg gcatgttcca attcactgtc taatattaaa taaggtgtca tgatgtaaac
      781 atattctcta gcatgattaa gaatatctat ataaacattt tcccctactt tatcagtgtc
      841 taaaggagaa tctccataag gaataacata gccttcattt tcaactaaat catcgtgtac
      901 accgaggtaa ggcgctacta acattttttc ctcagtaatg gaccacatct gtaagaaaag
      961 taggaggaaa ctatccactg ctttcccctt taacataata cctgaatctt tccaatgccc
     1021 aaataactca atgtgattaa tatactcatc ggctaagttt acaccaccag tcatgcctac
     1081 tactccatca ataacaacaa tttttcgatg gtcacgatag ttataataag ttgaaataaa
     1141 aggagaaatc ggagaaaatg cttttgcttt tattcctatt ttctcaagtc gtttcgtata
     1201 atcaaaggat aattttgtaa tttctatcat accatcatat aagatacgga cttcaacacc
     1261 ttcttccacc ttttgttcta aaatactaag tatttctccc cacattattc cttcgtcaat
     1321 gataaagaat tctaaaaaga tatacttctt agcttttagt agttcttctt tcattttttc
     1381 aaagaatgct tctcccgttg gtagaaatgt cacatcagta tttcgataaa ctgggaaatt
     1441 tccatgtgct ttttcaaaat attgaaccaa atgatatgtt gtggatgtac tcgacttaag
     1501 ctgattcaaa gtttcaggat catcctctag ataaatctga ctctcatcaa tcaatgtctc
     1561 caatctttgt ttcaaaccac gataacccca gtcgaatttg gtgtacatta ggaacatcgc
     1621 ccctagtaaa ggggcaatca ttaccaatat taaccaagtt actcgggaca aagcatccat
     1681 ttcactatta actaaatata agaccgctcc aattgttaag atatgctcaa cggttgctaa
     1741 ccaaacacga tactgctcta gccatgagta agaggctagt aaaaacaata attgtaaaat
     1801 caataaaata gcgataacgg ttgttcgact aaatataccc cttagtaaac ttttcttact
     1861 cttattaagt aaacgaacaa ttttgacttt attactgata agtttttccc ccctccaaat
     1921 caatcatttt attatagcat actgtcactt gaatagcatt ctattattct aaattatctc
     1981 tagttaaaat ctaatcccca ataaaaagag gctagcatca caagccctac ctctttatta
     2041 tttaaaataa tccaacggct gtaccgtctt ctaaaacatc catattgaga gctgcaggtt
     2101 ttttaggtaa accaggcatt gtcaatacgt ctccagtaag agcaactata aaaccagctc
     2161 ctgtttttgg aacaaattca cgaacagtta tatcaaagtc agttggagca ccaaataaat
     2221 ttggattatc agaaaagcta tattgtgtct ttgccataca aataggcatc ttgtcccagc
     2281 cattgtcact aaactctttt aattgtattt gtgccttagg tccaaaatga actttattac
     2341 caccatatat tttagtaaca atttttttaa ttttttcttc aatagtgtct tcatcattat
     2401 ataagcgttt gtaattggat gattgtgttt caatcacatt agcaacagtc tgtgcaagtt
     2461 caaggccacc gtctgctcca tcttcccaca cacttgctaa ttcaactgcc acatcaatat
     2521 tactacataa ggttttaaga gttgcaattt cactttctgt atctgcagta aactgattga
     2581 tagctacaac taccggaaca ccatattgac gcatattgtt aacgtgacgc tcgagatttg
     2641 taaaacctct tttaactgct tcgacgttct cttgtgagag atcttctttt gaaacacctc
     2701 catgcatttt caatgcacgc aatgtagcaa caataacaat cgcatcggga gaagtcggaa
     2761 ggtttggcgt ttttatatca agaaactttt cagcgcccaa gtctgctcca aaaccagctt
     2821 cagtaacaac gtaatctgct aagcgtagag ctgttgaagt cgctaagaca gaattacatc
     2881 catgtgcaat attagcaaat ggtcccccat ggacaagagc cggtgtccca taaatggttt
     2941 gaaccaaatt aggtttgata gcatctttta ggataagtgt gagagctcct tcaatcttca
     3001 aatctttaac atagattggt ttacggtccc ttgagtaagc cacaacaata tttgacaggc
     3061 gtttctttaa atctgatagg tcagtagcta aacatagaat agccattatt tcagaagcaa
     3121 cagtaatatc aaagccatct tcacgtggta tcccattgac agggcttcct aagccaacga
     3181 tcacctgacg aagagcacga tcattaagat caactacacg cttccaaatc actcgacgtt
     3241 ggtcaatatc taattcattg ccttgatgaa tatgattatc aagtagagct gataaagcat
     3301 tattagcagt tgtgattgcg tgcatatcac cagtaaaatg caaattaata tcttccattg
     3361 gtagtacttg tgcgtatcca cctccagcag ctcctccttt aatacccatt acaggaccta
     3421 aagaaggttc gcgaagcgca atcatcgttt ttttaccaat tttatttaaa gcatcagcta
     3481 atccaatgct catagtggat tttccctcac ctgctggtgt cggattaata gcagttacta
     3541 aaattaactt tcctactttt tgagatctta cagcttcaat cttatcaaaa gataattttg
     3601 ctttgtattt accatacaat tctatatcat caaaaccaat tcctacttgc tcaacaatct
     3661 cagcaatagg ctttagggcg acgctttgcg ctatttcaat atctgttttc atgatattct
     3721 cct
//
LOCUS       DATRQA010000156         3700 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.156, whole genome shotgun sequence.
ACCESSION   DATRQA010000156 DATRQA010000000
VERSION     DATRQA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.156"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            47..895
                     /locus_tag="VBD27_001814"
     CDS             47..895
                     /locus_tag="VBD27_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7025800.1"
                     /translation="MIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWI
                     IIAIGYGLGVMMPALMFGNVSGNHINPAFTLGLAFSGLFPWAHVGQYILAQILGAIFG
                     QLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSRKAAYINGFLNEFVGSFVLF
                     FGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVAS
                     LGGPTGPALNPARDLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVAL
                     FKLLYL"
     gene            complement(974..1870)
                     /locus_tag="VBD27_001815"
     CDS             complement(974..1870)
                     /locus_tag="VBD27_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO7025801.1"
                     /translation="MNETLLLHGIQLILIIAMIITFYQIVRHIRSQKINPFKRFFTGL
                     WIGFVTDALDTLGIGSFATTTTFFKLTKLVEDDRKIPATMTAAHVLPVLLQSLCFIFV
                     VKVEALTLITMAGAAFIGAFVGAKMTKNWHAPTVQRILGTLLITAAIIMLYRMITNPG
                     AGISDSVHGLHGIWLFVGIVFNFIIGVLMTMGLGNYAPELIFFSLMGLSPAVAMPVMM
                     LDAAMIMTASSTQFIKSGRVNWNGFAGLVTGGILGVIVAVLFLTNLDLNSLKTLVVGI
                     VLFTGAMLIRSSFKKTMPKSHH"
     gene            1995..2750
                     /locus_tag="VBD27_001816"
     CDS             1995..2750
                     /locus_tag="VBD27_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEO7025802.1"
                     /translation="MTLFHSLTFKHPVLRVNNRDLNIAFYQESLGFKLISEENAIAVF
                     SAWQNKEASFIIEESPTYRTRAVNGTKKLAKIIVKSQDAKDIEKLLANGAQAIQVYQG
                     QNGYAYETVSPEGDLFLLHAEDDLSQLVAIERPELEKKDDTTGLSNFAFQSISLNVPD
                     AVKAEAFYDKVFAGKFPINLSFKEAQGQDLQIAPNETWDIEILECCVNEDTNLNDLKS
                     TFESWGLDVYLDSKEKILVISDTSNIEIWISKE"
     gene            2747..3676
                     /locus_tag="VBD27_001817"
     CDS             2747..3676
                     /locus_tag="VBD27_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO7025803.1"
                     /translation="MIKTNTVLELIKEQIKDNLYHGASLAIYENGEWHEHYLGTIDGN
                     EKVKAGLVYDLASVSKVVGVGTLLAKLVYQGTIDIDKPLRYYYPTFHHQTLTVRQLAT
                     HSSGIDPFIPNRDQLNATQLKDAINHIKVLEDKSFKYTDINFLLLGFMLEEVLGDSLD
                     KLFKRYIFTPFQMKETSFGPRVEAVPTVAGINDGIVHDPKAKVLGKHTGSAGLFSTID
                     DLQRFSIHYLKDDFAKPLWNNYSLSKSRSLAWDIDKDWINHTGYTGPFIALNYQKQAA
                     AIFLTNRTFSYDDRPLWIKKRRHVQEAITSYFE"
BASE COUNT         1198 a          613 c          727 g         1162 t
ORIGIN      
        1 gtagaatggt taatgttgta aatatttttt ggggaggaaa caaggattga tcgaaatcac
       61 ttggactgtg aagtatatca cagaatttat cgcaaccgct tttcttatca tcttaggaaa
      121 tggggctgta gctaacgttg acttaaaagg cacaaaagga aataattcag gttggattat
      181 tattgcaatt ggttatggtt taggtgttat gatgccagcg cttatgtttg gtaatgtctc
      241 tggaaaccat attaacccag ctttcacact tggattggca ttttcaggtc ttttcccttg
      301 ggcacacgtt ggacaataca ttcttgcaca aattttagga gcaatatttg gacagttagt
      361 tgtcgttatg gtttatcaac cttactttgt taagacagaa aatccaaatc atgttcttgg
      421 atcattttca actatttctg cattagatga tggacagaaa tcaagtcgta aagcggctta
      481 cattaatggt ttcttaaatg aattcgttgg ttcttttgtt cttttctttg gagctttggc
      541 tttaacgaag aactattttg gtgtagaact tgttggtaaa cttgttcaag ctggttatga
      601 ccaaacgaca gcagcgacaa ggatttcacc atacgtaact ggttcacttg ctgtagctca
      661 cttaggtatt ggtttcttgg taatgactct agttgcttct ttaggtggac cgactggacc
      721 tgcattgaac cctgcgcgtg accttggtcc acgtattgtt caccgcttat taccaaaaca
      781 aattcttgga caagctaaag aagattcaaa atggtggtat gcatgggtgc cagttttagc
      841 accaatcgta gcaagtatac tcgctgtagc cttgttcaaa ctattatatc tttaatagtt
      901 aaagaaagat cggtttaccg gtcttttttt gttttaaata taaaaatcag ccagtcttgc
      961 tagctgattt cgtttaatga tgacttttgg gcatagtttt tttaaaggaa gaacgtatta
     1021 acattgctcc agtaaataaa acaataccta caaccagtgt tttcaagctg ttaagatcaa
     1081 ggttagttaa gaagagaaca gcgacaataa cgcctaatat tcccccagta accagaccgg
     1141 caaagccgtt ccaattaaca cgaccactct tgataaattg tgtactagaa gctgtcatta
     1201 tcatagctgc atctagcatc atgacaggca tagcaactgc aggactcagt cccattaggg
     1261 agaagaagat aagttcggga gcataattac ctagtcccat agtcattaat acaccaataa
     1321 taaaattgaa gacgatacca acaaataacc aaatgccgtg tagaccatgc actgaatctg
     1381 aaataccagc gccagggtta gtgatcatac gatagagcat aataatagca gctgtgatga
     1441 gcagtgttcc tagaatacgt tgaacagtag gagcgtgcca atttttagtc attttagctc
     1501 caacaaaagc cccaataaaa gctgcaccag ccattgttat tagtgttaaa gcctctactt
     1561 ttacaacaaa aataaaacag agtgactgca acaaaactgg aagtacgtgt gcagctgtca
     1621 ttgttgcggg tatcttacga tcgtcttcaa caagcttagt tagtttaaaa aatgtggtcg
     1681 ttgttgcaaa agagccgata ccaagagtat cgagagcatc agttacaaaa ccgatccaca
     1741 aaccagtgaa aaatcgtttg aaaggattta ttttttgaga cctaatatgt ctaacaatct
     1801 gataaaaagt gataatcatg gcgataatca gaataagctg aatcccatgt aatagtagtg
     1861 tttcattcat aatatccagt ctacaaaaaa atagtgaaac tgtcaatttg actttatggt
     1921 attgagagca caaagattct tgctaaatta tctaaaatta tctaaaatag actagactga
     1981 acaaggagga atttatgacg ttatttcatt cattgacatt taaacaccca gtattacgtg
     2041 ttaataatag agacttgaat attgcatttt atcaggagtc tcttggattt aaattgataa
     2101 gtgaagaaaa tgcaatcgca gttttctcag catggcagaa caaagaggct agctttatta
     2161 ttgaggaatc cccaacttat cgtactcgtg cggtgaatgg tactaagaag ttagcaaaga
     2221 ttattgttaa gtctcaagat gcaaaagata ttgaaaagct tttggcaaat ggtgcgcaag
     2281 ccattcaagt atatcaaggt caaaatggtt atgcctatga aacagtttcg cccgagggag
     2341 accttttctt acttcatgct gaagatgatt tgtcacaatt agtggcaatt gagcgcccag
     2401 agttagaaaa aaaagatgac actacaggtc tatcaaactt tgctttccaa tctattagtt
     2461 tgaatgtacc tgatgccgtg aaagctgaag ctttttacga taaagtattt gctgggaagt
     2521 tcccaattaa tttatctttc aaagaagcac agggacaaga tttgcaaatt gctccaaatg
     2581 aaacttggga tattgagata ctagaatgct gtgttaatga agatactaat ttaaatgact
     2641 tgaaatcaac ttttgaatca tggggacttg atgtttacct tgattctaaa gaaaagattt
     2701 tagtcatctc tgacactagt aatattgaga tttggatttc aaaagaatga ttaaaactaa
     2761 taccgtttta gaactcatta aggagcaaat caaagataat ctttatcatg gtgcaagctt
     2821 agctatttat gaaaatggag agtggcacga acattacttg gggacaattg atggcaatga
     2881 gaaagttaaa gcgggattgg tctatgatct agccagtgta tcaaaagttg tcggcgtggg
     2941 aactttacta gcaaagttag tttatcaagg gactattgat attgataagc cattaaggta
     3001 ttattaccct acgtttcatc atcagacgtt aactgtaagg caattagcta cacatagcag
     3061 tggaattgac ccttttattc caaatcgtga tcagttgaat gctactcagc tgaaagatgc
     3121 cattaatcat atcaaggttt tggaagataa atcgtttaaa tacactgata ttaattttct
     3181 tttacttggt tttatgttag aagaggtatt aggggatagt ttggataaac ttttcaaacg
     3241 ttatattttc actccttttc agatgaaaga aacctctttt ggtcctagag tagaagctgt
     3301 tccaactgta gccggtatta acgatggtat tgtacacgat cctaaagcta aggtattagg
     3361 aaagcatact ggttctgcag gtttattttc aactattgat gatttacaaa gattttctat
     3421 tcattattta aaagatgatt ttgcgaagcc tttgtggaat aattatagtt tgagtaaatc
     3481 aagatcatta gcttgggata tagataagga ttggataaac catactggtt atactggtcc
     3541 ttttattgca ctaaattatc aaaagcaggc agcagctatt tttttgacaa acagaacctt
     3601 ttcctatgat gaccgtccac tttggattaa gaaacgacga catgtacaag aggcgataac
     3661 atcgtacttt gaataaaaaa cttctcctag aattttctag
//
LOCUS       DATRQA010000157         3593 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.157, whole genome shotgun sequence.
ACCESSION   DATRQA010000157 DATRQA010000000
VERSION     DATRQA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.157"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            109..528
                     /locus_tag="VBD27_001818"
     CDS             109..528
                     /locus_tag="VBD27_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7025804.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDRKISELYHVSRSAICSRRTRGLRKLKMLLSEGK"
     gene            533..772
                     /locus_tag="VBD27_001819"
     CDS             533..772
                     /locus_tag="VBD27_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7025805.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(957..1523)
                     /locus_tag="VBD27_001820"
     CDS             complement(957..1523)
                     /locus_tag="VBD27_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO7025806.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDAKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(1690..2709)
                     /locus_tag="VBD27_001821"
     CDS             complement(1690..2709)
                     /locus_tag="VBD27_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO7025807.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
     gene            complement(2807..3511)
                     /locus_tag="VBD27_001822"
     CDS             complement(2807..3511)
                     /locus_tag="VBD27_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7025808.1"
                     /translation="MRTVLVVQGDDETIELLRSYLEGALYKVVMASDGEEAFSLFQQH
                     QIDLAIIDITLPKIDGYELTRLIRQDSQIPIIMLAAKTTDMDRILGLNIGADDFITKP
                     FNSLEVLARINSQLRRYYEFNSLAKPKNQFIKIGELELDEEHVELTKNGKHIKLTATE
                     FKILHILMSSPGKIYTKTQLYEKINGRYLEGDETTIMVHISNIRDKIEDDSKYPKYIK
                     TLRGVGYKIEKPQYLV"
BASE COUNT         1135 a          633 c          554 g         1271 t
ORIGIN      
        1 aatatatgtt ttgtgtgggc ttttttctta ccatttttct gtatttctaa agggtataaa
       61 cggtattccg tttatatatt tcagcattag cagaaagggg gtgagattat gaggacatct
      121 tctttcgagc atatcgtcag attacagttc aattctttaa tgctgattgt cattaagaac
      181 aaagtgaaaa gtcgaaacag acagcttgca agacattcta aacgtgaagt attgttttgt
      241 gaaatagccg aaacgaaaca agttgaacgt ggaacgattg atacctgttt ttatgattgt
      301 gtatcattta aagtcttgca ctttacaatt tatgtatctg atgaaacact tggtacagtt
      361 ttacatagac tgtcagaaaa acagcgtagt gcgattttat tacgttattt tcaaggaatg
      421 aacgacagaa aaatttcaga actgtaccat gtatcacgtt ctgctatttg cagtagaaga
      481 actagaggac taaggaagtt aaaaatgctt ttgagcgaag ggaagtaaag aaatgagtaa
      541 agattatggc tatccctctt ttgaattgat ttgcaaagcg tcaagcggag atgaaatggc
      601 agttaaagag attttgaagt tttatgacgg ttatatttct aaattatgct tgcgtccttt
      661 ttaccactct gaaagtggta aaatcattat gcaggttgat gaagaactaa aaggagaaat
      721 tcatacagac atgatgaaag caatattgaa atttgaaatc ggagtgaagt aatcaactgt
      781 ataaaaaaat cgggagatac acagtttcgt gctatctccc ttagtagttt tacggtcata
      841 tcaaggctca ttcaatcgtg gtaaattcgt ggtaagatga gtgtttttct atacttcaaa
      901 atgcttgaat gtatagtgtt tatgcggata attgaaaatt agatataaaa tttctattaa
      961 tcattcatat gatctctaga acgttcaatg atttttccag tttctgcatc aatttgataa
     1021 tcaacttcat ttttattttt caggtccaca aagtcaatat cataaactga ttttccattt
     1081 tcaatttctt ctttgatctt aagattactg acttctgatt cctctatatt agcatcttta
     1141 aatgcaatct ttttggcttc ttcttgactt attttatgag atttttgact tgacgatgaa
     1201 cttgactgag aagtcgattt tgaaaagcca ttattatcca tgtcccgctc aaggatttgt
     1261 ccagtcttag catctacatg atagctgtat tctgcgcttt tggtattgaa ttcaatttcg
     1321 tagctccctc taaagttttc aaattctttg tcaatttttt gatgtatgac atccttctta
     1381 gccactttag catctttata agcaatagct aacgcttgat tagttgtctt ttggaattgt
     1441 gattggtaaa ataatacacc acctccaagg ataacaacaa ggactaaaag aactcctaaa
     1501 aaaactttat ttttcttaat catacagcac cttctttcta ttattcaatt gtattttaac
     1561 ataagctaga cactttttca attttgacca ctcgtttata aaaatatgtg gtcaataaaa
     1621 accatttgac attaataccg caaattaaaa gataccatag tatcatggta tcttttaata
     1681 gtatatgtat cagttgatgc gcattttctg cacagccttc ttcaagtatt tgacttgttt
     1741 atcaaccgat tgcttagcac tacgccttcc aaatgcgtaa tatccttttt tattgaaata
     1801 actacaatcg taatcctctt ctttaaagaa aaaccatttc cttctagata gatttggcga
     1861 tctataacct ctagtcatat cgtttcgaaa ttttgggtct aactttacag gatcaacatc
     1921 taattcttta tgacgtgtat tatttttaaa agcataatta aagctagcac cgttgataat
     1981 gccattacgt gtgattactt ctgcgtcaat ctcgtttaaa aagtttcctt tgtcatcaat
     2041 aataaattcc gtatggaatg aaattaaaag cttactatta ataaccggct catcatctgg
     2101 gaaaccaaag atttcaccat ttctccgacg tttattatgc aagcgagcag attcatagta
     2161 ggcgatacta tctggttctc ttaaacatga taggtagttt actaaagcat ctctatcagt
     2221 cataccaaca tctttaaagt atcgtcgaac atactgtgct tgctgactag aaatcaaata
     2281 gcggaattga tggagtttcc tatttaatac agtatcttcg gctagactat tcccaggaaa
     2341 agaatcataa ataacttgtg ataaggctat ccagaaatca tcatgcggag ctaactcatc
     2401 tgaaaactgt gcgaccaagt attgtgtttc tgataattct cctgacaatt cagttggcat
     2461 tttagcctgc tctaacacga attctaaagc tttgaggggt gcttgtaaac ttaaagaagt
     2521 ccaaaaatac ttaaaaatag gagagccaac ttttatatca tcaatagcct tgagtccctt
     2581 taaaaagagt aatagatcat ctttaggtat gctccattta tctgctaaag acagtatcat
     2641 ttttatattt tgataataat tttctatccc ttcaactgta aaaggaatat taagagcggt
     2701 ctttgccata tctctcccct ccaattttac ttattatagc aaaaaggaaa gcttaaaacc
     2761 aagaaatatc ttcactaaaa aatggctctc actagccatt atgtttttaa acaagatact
     2821 gcggtttttc gattttatac cctactcctc taagtgtttt tatatattta ggatatttac
     2881 tatcatcttc gattttgtca cggatattag aaatatgcac cataatcgtt gtctcatcac
     2941 cttctaaata cctaccatta atcttctcat agagttgtgt tttagtataa atctttcctg
     3001 gtgatgacat taagatatga agaatcttaa attctgtagc agtcaacttg atatgtttac
     3061 catttttagt tagttcaaca tgttcctcat ctagttccaa ttcccctatc tttataaatt
     3121 gatttttagg tttggcaaga ctattgaatt catagtaacg acgcagttgc gaattaattc
     3181 gagctaaaac ctccaaagaa ttaaacggtt tcgtgataaa atcatctgct ccaatattca
     3241 atcctaatat tctgtccata tctgttgtct tagctgctag cataataata ggaatctgac
     3301 tatcttgacg aatgagtcgc gtcaactcat atccatctat tttaggtagc gtaatatcta
     3361 tgattgctaa atcaatctga tgttgctgaa ataaagaaaa agcctcctca ccatcactag
     3421 ccatcacaac cttataaaga gctccctcta aatacgacct taataactct attgtttcgt
     3481 catcaccttg cactactaat actgttcgca atgctccatc ccccttttta ttaatactat
     3541 tttcctttat gtgtcaatca tattaattat accaaaaaat ctaattttgt gaa
//
LOCUS       DATRQA010000158         3585 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.158, whole genome shotgun sequence.
ACCESSION   DATRQA010000158 DATRQA010000000
VERSION     DATRQA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.158"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..232
                     /locus_tag="VBD27_001823"
     rRNA            <1..232
                     /locus_tag="VBD27_001823"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            287..359
                     /locus_tag="VBD27_001824"
     tRNA            287..359
                     /locus_tag="VBD27_001824"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:320..322,aa:Ala,seq:tgc)
     gene            514..3416
                     /locus_tag="VBD27_001825"
     rRNA            514..3416
                     /locus_tag="VBD27_001825"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3491..>3585
                     /gene="rrf"
                     /locus_tag="VBD27_001826"
     rRNA            3491..>3585
                     /gene="rrf"
                     /locus_tag="VBD27_001826"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1024 a          722 c         1023 g          816 t
ORIGIN      
        1 ggctgcaact cgcctacatg aagtcggaat cgctagtaat cgcggatcag cacgccgcgg
       61 tgaatacgtt cccgggcctt gtacacaccg cccgtcacac cacgagagtt tgtaacaccc
      121 gaagtcggtg aggtaacctt ttaggagcca gccgcctaag gtgggataga tgattggggt
      181 gaagtcgtaa caaggtagcc gtatcggaag gtgcggctgg atcacctcct ttctaaggat
      241 aaggaaacct gccatttgcg tcttgtttag ttttgagagg tcttgtgggg ccttagctca
      301 gctgggagag cgcctgcttt gcacgcagga ggtcagcggt tcgatcccgc taggctccat
      361 tgaatcgaaa ggttcaaatt gttcattgaa aattgaatat ctatatcaaa ttccacgatc
      421 tagaaataga ttgtagaaag taacaagaaa ataaaccgaa aacgctgtga atatttaatg
      481 agttttctag ttttaaagaa actagtttaa taaggttaag ttaataaggg cgcacggtgg
      541 atgccttggc actagaagcc gaagaaggac gtgactaacg acgaaatgct ttggggagct
      601 gtaagtaagc gctgatccag agatgtccga atgggggaac ccactagtta atgactagta
      661 tccatatctg ttaaggatat gagaaggaag acgcagtgaa ctgaaacatc taagtagctg
      721 caggaagaga aagcaaaagc gattgcctta gtagcggcga gcgaaacggc aggagggcaa
      781 accgaagtgt ttacacttcg gggttgtagg actgcgatat aggattaatc attatagaag
      841 aattacctgg gaaggtaagc caaagagagt aacagcctcg tatttaaaat gatagattaa
      901 ccttagcagt atcctgagta cggcgagaca cgcgaaatct cgtcggaatc tgggaggacc
      961 atctcccaac cctaaatact ctctagtgac cgatagtgaa ccagtaccgt gagggaaagg
     1021 tgaaaagcac cccgggaggg gagtgaaata gaacctgaaa ccgtgtgcct acaacaagtt
     1081 cgagcccgtt aatgggtgag agcgtgcctt ttgtagaatg aaccggcgag ttacgatatg
     1141 atgcgaggtt aagttgaaga gacggagccg tagggaaacc gagtcttaat agggcgccat
     1201 agtatcatgt tgtagacccg aaaccatgtg acctacccat gagcaggttg aaggtgaggt
     1261 aaaactcact ggaggaccga accagggcac gttgaaaagt gcttggatga cttgtgggta
     1321 gcggagaaat tccaaacgaa cttggagata gctggttctc tccgaaatag ctttagggct
     1381 agcgtcgatg ttaagtctct tggaggtaga gcactgtttg ggtgaggggt ccatcccgga
     1441 ttaccaatct cagataaact ccgaatgcca acgagatata atcggcagtc agactgcgag
     1501 tgctaagatc cgtagtcgaa agggaaacag cccagaccac cagctaaggt cccaaaatat
     1561 atgttaagtg gaaaaggatg tggggttgca cagacaacta ggatgttagc ttagaagcag
     1621 ctattcattc aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1681 ccggggctaa aacatattac cgaagctgtg gatgacacaa aagtgtcatg gtaggagagc
     1741 gttctatgtg tgaagaaggt gtaccgtgag gagcgctgga acgcatagaa gtgagaatgc
     1801 cggtatgagt agcgcaagac aggtgagaat cctgtccacc gtaagactaa ggtttccagg
     1861 ggaaggctcg tccgccctgg gttagtcggg acctaaggag agaccgaaag gtgtatccga
     1921 tggacaacag gttgatattc ctgtactaga gtatatagtg atggagggac gcagtaggct
     1981 aactaaacca gacgattgga agtgtctggt caaacagtga ggtgtgatat gagtcaaatg
     2041 cttatatctt taacattgag ctgtgacgag gagcgaagtt tagtagcgaa gttagtgatg
     2101 tcacactgcc aagaaaagct tctagcgata catatactct acccgtaccg caaaccgaca
     2161 caggtagtcg aggcgagtag cctcaggtga gcgagagaac tctcgttaag gaactcggca
     2221 aaatggcccc gtaacttcgg gagaaggggc gctgatttta gatcagccgc agtgaatagg
     2281 cccaagcaac tgtttatcaa aaacacagct ctctgctaaa tcgtaagatg atgtataggg
     2341 ggtgacgcct gcccggtgct ggaaggttaa gaggagtgct tagcgtaagc gaaggtatga
     2401 attgaagccc cagtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt
     2461 gtcgggtaag ttccgacccg cacgaaaggc gtaatgattt gggcactgtc tcaacgagag
     2521 actcggtgaa attttagtac ctgtgaagat gcaggttacc cgcgacagga cggaaagacc
     2581 ccatggagct ttactgcagt ttgatattga gtatctgtac cacatgtaca ggataggtag
     2641 gagcctatga taacgggacg ccagtttcgt ttgaggcgtt gttgggatac tacccttgtg
     2701 ttatggctac tctaacccag ataggcaatc cctatcggag acagtgtctg acgggcagtt
     2761 tgactggggc ggtcgcctcc taaaaggtaa cggaggcgcc caaaggttcc ctcagactgg
     2821 ttggaaatca gtcgtagagt gtaaaggtat aagggagctt gactgcgaga gctacaactc
     2881 gagcagggac gaaagtcggg cttagtgatc cggtggttcc gtatggaagg gccatcgctc
     2941 aacggataaa agctaccctg gggataacag gcttatctcc cccaagagtt cacatcgacg
     3001 gggaggtttg gcacctcgat gtcggctcgt cgcatcctgg ggctgtagtc ggtcccaagg
     3061 gttgggctgt tcgcccatta aagcggcacg cgagctgggt tcagaacgtc gtgagacagt
     3121 tcggtcccta tccgtcgcgg gcgtaggaaa tttgagagga tctgctccta gtacgagagg
     3181 accagagtgg acttaccgct ggtgtaccag ttgtcttgcc aaaggcatcg ctgggtagct
     3241 atgtagggaa gggataagcg ctgaaagcat ctaagtgcga agcccacctc aagatgagat
     3301 ttcccatgat tttatatcag taagagccct gagagatgat caggtagata ggttaggagt
     3361 gtaattgtgg tgacacattt agcggactaa tactaatagc tcgaggactt atccaaaaag
     3421 agatattgac aacgttacgg tttcttgtta gactatagat attcaatttt gagtgaataa
     3481 gactcaatag ttaagtgata attgcctagg agatacacct gttctcatgc cgaacacaga
     3541 agttaagccc tagaacgccg gaagtagttg ggggttgccc cctgt
//
LOCUS       DATRQA010000159         3492 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.159, whole genome shotgun sequence.
ACCESSION   DATRQA010000159 DATRQA010000000
VERSION     DATRQA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.159"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            171..992
                     /gene="mutM"
                     /locus_tag="VBD27_001827"
     CDS             171..992
                     /gene="mutM"
                     /locus_tag="VBD27_001827"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEO7025809.1"
                     /translation="MPELPEVETVRKGLERLVVNQEIASITIKVPKMVKTDLNDFMIS
                     LPGKTIQQVLRRGKYLLFDFGEMVMVSHLRMEGKYLLFPNKVPDNKHFHLYFKLTNGS
                     TLVYQDVRKFGTFELVRKSSLKDYFTQKKLGPEPTADTFQFEPFSKGLANSKKPIKPL
                     LLDQRLVAGLGNIYVDEVLWAAKIHPQRLANQLTESETSLLHKEIIRILTLGIEKGGS
                     TIRTYKNALGEDGTMQKYLQVYGKTGQPCPRCGCLIKKIKVGGRGTHYCPRCQCL"
     gene            983..1576
                     /locus_tag="VBD27_001828"
     CDS             983..1576
                     /locus_tag="VBD27_001828"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO7025810.1"
                     /translation="MLMTKIIGLTGGIASGKSTVTKIIRESGFKVIDADQVVHKLQAK
                     GGKLYQALLEWLGPEILDADGELDRPKLSQMIFANPDNMKTSARLQNSIIRQELACQR
                     DQLKQTEEIFFMDIPLLIEEKYIKWFDEIWLVFVDKEKQLQRLMARNNYSREEAELRL
                     SHQMPLTDKKSFASLIIDNNGDLITLKEQILDALQRL"
     gene            1700..2401
                     /locus_tag="VBD27_001829"
     CDS             1700..2401
                     /locus_tag="VBD27_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025811.1"
                     /translation="MELVIRDIRKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTL
                     FNILYGDLAADNGTICLLKDNHEYPLTDKDIGIVYSENYLPEFLTGYEFVKFYMDLHP
                     SDDLMTIDDYLDFMEIGQTERHRIIKGYSDGMKSKLSLICLMISKPKVILLDEPLTAV
                     DVVSSIAIKRLLLELSEDHIIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQF
                     EERLLQVLKGDEYDK"
     gene            2626..>3492
                     /locus_tag="VBD27_001830"
     CDS             2626..>3492
                     /locus_tag="VBD27_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001882372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025812.1"
                     /translation="MKQLNILPQEGFTLTLTLMVITSLLIVINFYIKLIEPNDMQQTH
                     MIRLFRIEPSRYFQSAELLEASSELFFRSLSFGIFFHLNHFAFWHGLTFALFLAGSRL
                     GIKVLCLPLYGRGKDQENPESLLNILFYLGIGIGVFLSLFFLLLGKQFSPYPFFSWYT
                     GIAGLIIWLVSYYRLKTYSKLNHLTYLTLTHETMKEKIAKIDNIELLGIEMKDRDINV
                     SELSPLLFENLSGISYLNAIFLKRMSRYLQRKILVRLAVLSVLFGIELLFLLFFKEKI
                     NIPMNEHSFSKFL"
BASE COUNT         1174 a          510 c          666 g         1142 t
ORIGIN      
        1 tggcaataat aaagaatgct aatgagtgca agtttatgag taatttttaa agcttttgtc
       61 tcaaaatgat aataagaaag gaaaatagaa atcgaatggt caggtgacat caagaacgtt
      121 tggaaattat tgctctaagc aatgactatt gatttttaga ctatatattt atgccagaat
      181 taccagaagt agaaaccgtt agaaaaggac tagaaagact tgtagttaat caagaaatag
      241 cttctataac gattaaagtc cctaaaatgg tgaaaactga tttgaatgac tttatgataa
      301 gtctaccagg aaaaacaatt caacaggtat tgagacgagg gaagtatctt ctttttgatt
      361 ttggtgaaat ggttatggta tcacatttac gcatggaagg aaaatatcta ctcttcccaa
      421 ataaagtacc agataacaaa cattttcacc tttatttcaa attgactaat ggcagcacat
      481 tggtttacca agatgtccgt aaatttggga catttgagtt ggttcgtaaa tcatctctca
      541 aagattattt tactcaaaaa aaattaggtc ctgagccaac tgctgacaca tttcaatttg
      601 agcctttttc aaaaggctta gcaaactcca aaaagccaat aaaaccttta ctattagatc
      661 agagattagt agcaggactt ggaaatattt acgtagatga agtcttgtgg gcagcaaaaa
      721 ttcacccaca gcgtttggct aatcaattga ccgaatctga aacaagctta ttacacaagg
      781 aaattattcg cattttaact ttaggcatcg aaaaaggtgg ctccacgatt agaacatata
      841 agaatgctct tggagaggac ggtacgatgc aaaagtattt acaggtctat ggtaaaactg
      901 ggcaaccgtg tccaaggtgt ggttgtttaa ttaagaagat aaaagtagga gggagaggga
      961 cgcattattg ccctcggtgt caatgcttat gacaaaaata ataggactga caggagggat
     1021 agcttctgga aagtcaacgg taacaaaaat aatacgagaa tcaggtttta aagtcataga
     1081 tgcggatcaa gtggttcata aattgcaagc taagggtggg aaactttacc aagctttatt
     1141 agaatggttg ggtcccgaga tacttgatgc tgatggtgag ttggatagac caaagctttc
     1201 tcaaatgatt tttgctaatc cagacaatat gaagacatca gctaggctac aaaatagtat
     1261 cattcgtcaa gagttagcat gtcagcgcga ccaattaaaa caaacagaag agatattttt
     1321 catggatatt cctttattga ttgaagaaaa gtatataaaa tggtttgatg agatttggtt
     1381 ggtatttgtt gataaagaaa aacaattaca acgattaatg gcccgtaaca actacagtcg
     1441 agaagaagca gaattacgac tttcacacca aatgccttta acagataaaa aaagtttcgc
     1501 tagtcttatt attgacaata atggtgattt aataacttta aaagagcaaa tattggatgc
     1561 tcttcaacgt ttatagaact actaaatgga taatgtattt atccattttt tgtctttatt
     1621 acactagttt taaaaaactt gcgattaaac cacagtaata gtacaatgat ataaaaggtt
     1681 tactttcaaa ggagttttta tggagttagt aattagagat attcgtaagc ggtttcagga
     1741 aacagaggtc ttgagaggag caagttaccg attttattca ggtaaaataa caggggtctt
     1801 aggtaggaat ggtgctggga aaacaacttt atttaatata ctttatgggg atcttgcagc
     1861 tgacaacggg accatttgtt tattgaagga taatcacgag tatcctctta ccgataagga
     1921 tattggtatt gtttattccg aaaactacct tccagaattt ttaacagggt atgaatttgt
     1981 aaaattttac atggatttac atccttcaga tgatttaatg acaatagatg attatttaga
     2041 ttttatggaa ataggacaaa cagagcgtca tagaattatc aaaggatatt ctgatggaat
     2101 gaagagtaag ctctcattaa tttgcctgat gatttctaag ccaaaagtaa ttttactaga
     2161 tgagccactg actgcagttg atgttgtatc aagtattgca ataaaacgcc ttttgttgga
     2221 attaagtgag gatcatatta ttatattatc aactcatata atggccttag cagaagatct
     2281 atgtgatatt gtggctgtat tagacaaagg aaaactccaa acattagata ttgatcgtaa
     2341 acatgaacaa ttcgaagagc gtcttcttca agtgttgaag ggagatgaat atgacaagta
     2401 aagcattaat atggtctata ttacagattg atagtgctag agcttataat agttttttat
     2461 attaccttca gaaaatcccc tggttgggaa gaaaaatccc taaccgatgg tataaaaggt
     2521 gtagtactat ttattcgtat cattttcttt ttcttaagag gttttggtcg ttccataggt
     2581 tacgtactat attgttatgt tttatcattt tttgttagaa gtggcatgaa gcagttaaat
     2641 attttaccgc aagaaggttt taccctaact ttaacattga tggtgattac ctctctttta
     2701 attgtcataa atttctatat aaaacttata gaacctaatg atatgcaaca aactcatatg
     2761 attcgccttt tcagaattga gccatcgagg tatttccaat ccgctgaact tttagaagcc
     2821 agtagtgaac tattttttag aagtttgagc tttggcatat ttttccattt aaatcacttt
     2881 gctttttggc atggtttgac ttttgcttta tttttagcag gtagtcgcct tggaatcaag
     2941 gtgctctgtt tacctttata tggtagaggt aaagaccaag agaatccaga gtctttacta
     3001 aatattttat tttacttagg gattggtata ggagtcttcc tttctctctt tttccttctt
     3061 cttggcaaac agttctctcc atatccattt ttttcatggt acaccggtat tgcaggactt
     3121 atcatatggt tagttagtta ctatcgatta aaaacttatt ctaaacttaa ccacttgacc
     3181 taccttaccc taacgcatga gacgatgaag gagaaaattg caaagattga taatatagag
     3241 ctgcttggga ttgaaatgaa agatagggat ataaatgtca gtgaactttc tcccctgcta
     3301 tttgaaaatt tatcaggtat ttcatattta aacgctatct ttttaaaacg gatgagtaga
     3361 tacttgcaaa ggaaaatttt ggtacgttta gctgttttaa gtgttctgtt tggaatagaa
     3421 ctccttttcc tgctattttt taaagaaaag ataaatattc cgatgaatga gcatagtttt
     3481 tcaaaattcc ta
//
LOCUS       DATRQA010000160         3418 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.160, whole genome shotgun sequence.
ACCESSION   DATRQA010000160 DATRQA010000000
VERSION     DATRQA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.160"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            12..1382
                     /locus_tag="VBD27_001831"
     CDS             12..1382
                     /locus_tag="VBD27_001831"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO7025813.1"
                     /translation="MDKHHSKKAILKLTLITTSILLMHSNQVNAEEQELKNQEQSPVI
                     ANVAQQPSPSVTTNIVEKTSVTAASASNTVKEMGDTSVKNDKTEDELLEELSKNLDTS
                     NLGADLEEEYPSKPETTNNKESNVVTNASTAIAQKVPSAYEEVKSESKSSLAVLDTSK
                     ITKLQATTQRGKGNVVAIIDTGFDINHDIFRLDSPKDDKHSFKTKAEFEELKAKHNIT
                     YGKWVNDKIVFAHNYANNTETVADIAAAMKDGYGSEAKNILHGTHVAGIFVGNSKRPA
                     INSLLLEGAAPNAQVLLMRIPDKIDSDKFGEAYAKAIIDAVNLGAKTINMSLGKTADS
                     LIALNDKVKLALKLASEKGVAVVVAAGNEGAFGMDYSKPLSTNPDYGTVNSPAISEDT
                     LSVASYESLKTISEVVETTIEGKLVKLPIVTSKPFDKGKAYDVVYANYGAKKILKVRT
                     LKVRLH"
     gene            1409..1726
                     /locus_tag="VBD27_001832"
     CDS             1409..1726
                     /locus_tag="VBD27_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEO7025814.1"
                     /translation="MTKITHATNAGVVGIVIFNDQEKRGNFLIPYRELPVGVISKVDG
                     ERIKNTSSQLTFNQSFEVVDSQGGNRMLEQSSWGVTAEGAIKPDVTASGFEIYSSTYN
                     NQY"
     gene            1745..>3418
                     /locus_tag="VBD27_001833"
     CDS             1745..>3418
                     /locus_tag="VBD27_001833"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fn3-like domain-containing protein"
                     /protein_id="HEO7025815.1"
                     /translation="MASPHVAGLMTMLQSHLAEKYKGMNLDSKKLLELSKNILMSSAT
                     ALYSEEDKAFYSPRQQGAGVVDAEKAIQAQYYVTGNDGKVKINLKREGDKFDITVTIH
                     KLVEGVKELYYQANVATEQVNKGKFALKPQALLDTNWQKVILRDKETQVRFTIDASQF
                     SQKLKEQMANGYFLEGFVRFKEAKDSNQELMSIPFVGFNGDFANLQALETPIYKTLSK
                     GSFYYKPNDTTHKDQLEYNESAPFESNNYTALLTQSASWGYVDYVKNGGELELAPESP
                     KRIILGTFENKVEDKTIHLLERDAANNPYFAISPNKDGNRDEITPQATFLRNVKDISA
                     QVLDQNGNVIWQSKVLPSYRKNFHNNPKQSDGHYRMDALQWSGLDKDGKVVADGFYTY
                     RLRYTPVAEGANSQESDFKVQVSTKSPNLPSRAQFDETNRTLSLAMPKESSYVPTYRL
                     QLVLSHVVKDEEYGDETSYHYFHIDQEGKVTLPKTVKIGESEVAVDPKALTLVVEDKA
                     GNFATVKLSDLLNKAVVSEKENAIVISNSFKYFDNLKKEPMFISKEGKVV"
BASE COUNT         1220 a          519 c          694 g          985 t
ORIGIN      
        1 aaataggaaa agtggataaa catcactcaa aaaaggctat tttaaagtta acacttataa
       61 caactagtat tttattaatg catagcaatc aagtgaatgc agaggagcaa gaattaaaaa
      121 accaagagca atcacctgta attgctaatg ttgctcaaca gccatcgcca tcggtaacta
      181 ctaatattgt tgaaaaaaca tctgtaacag ctgcttctgc tagtaataca gtgaaagaaa
      241 tgggtgatac atctgtaaaa aatgacaaaa cagaagatga attattagaa gagttatcta
      301 aaaaccttga tacgtctaat ttgggggctg atcttgaaga agaatatccc tctaaaccag
      361 agacaaccaa caataaagaa agcaatgtag taacaaatgc ttcaactgca atagcacaga
      421 aagttccctc agcatatgaa gaggtgaagt cagaaagcaa gtcatcgctt gctgttcttg
      481 atacatctaa aataacaaaa ttacaagcca caacccaaag aggaaaggga aatgtagtag
      541 ctattattga tactggcttt gatattaacc atgatatttt tcgtttagat agcccaaaag
      601 atgataagca cagctttaaa actaaggcag aatttgagga attaaaagca aaacataata
      661 tcacttatgg gaaatgggtt aacgataaga ttgtttttgc acataactac gccaacaata
      721 cagaaacggt ggctgatatt gcagcagcta tgaaagatgg ttatgggtca gaagcaaaga
      781 atattttgca tggtacacac gttgctggta tttttgtagg taatagtaaa cgtccagcaa
      841 tcaatagtct tcttttagaa ggtgcagcgc caaatgctca agtcttatta atgcgtattc
      901 cagataaaat tgattcggac aaatttggag aagcatatgc taaagcaatc atagacgctg
      961 ttaatctagg agcaaaaacg attaatatga gcctgggaaa aacggctgat tctttaattg
     1021 ctctcaatga taaagttaaa ttagcactta aattagcttc tgagaagggc gttgcagttg
     1081 ttgtggctgc cggaaatgaa ggtgcatttg gtatggatta tagcaaacca ttatcaacta
     1141 atcctgacta cggtacggtt aatagtccag ctatttctga agatactttg agtgttgcta
     1201 gctatgaatc acttaaaact atcagtgagg tcgttgaaac aactattgaa ggtaagttag
     1261 ttaagttgcc gattgtgact tctaaacctt ttgacaaagg taaggcctac gatgtggttt
     1321 atgccaatta tggtgcaaaa aagattttga aggtaaggac tttaaaggta agattgcatt
     1381 aattgagcgt ggtggtggac ttgattttat gactaaaatc actcatgcta caaatgcagg
     1441 tgttgttggt atcgttattt ttaacgatca agaaaaacgt ggaaattttc taattcctta
     1501 ccgtgaatta cctgtggggg ttattagtaa agtagatggc gagcgtataa aaaatacttc
     1561 aagtcagtta acatttaacc agagttttga agtagttgat agccaaggtg gcaatcgtat
     1621 gctggaacaa tcaagttggg gcgtgacagc tgaaggagca atcaagcctg atgtaacagc
     1681 ttctggcttt gaaatttatt cttcaaccta taataatcaa tactaaacaa tgtctggtac
     1741 aagtatggct tcaccacatg ttgcaggatt aatgacaatg cttcaaagtc atttggctga
     1801 gaaatataaa gggatgaatt tagattctaa aaaattgcta gaattgtcta aaaacatcct
     1861 catgagctca gcaacagcat tatatagtga agaggataag gcgttttatt caccacgtca
     1921 gcaaggtgca ggtgtagttg atgctgaaaa agctatccaa gctcaatatt atgttactgg
     1981 aaacgatggc aaagttaaaa ttaatctcaa acgagaggga gataaatttg atatcacagt
     2041 tacaattcat aaacttgtag aaggtgtcaa agaattgtat tatcaagcta atgtagcaac
     2101 agaacaagta aataaaggta aatttgccct taaaccacaa gccttgctag atactaattg
     2161 gcagaaagta attcttcgtg ataaagaaac acaagttcga tttactattg atgctagtca
     2221 atttagtcag aaattaaaag aacagatggc aaatggttat ttcttagaag gttttgtacg
     2281 ttttaaagaa gccaaggata gtaatcagga gttaatgagt attccttttg taggatttaa
     2341 tggtgatttt gcgaacttac aagcacttga aacaccgatt tataagacgc tttctaaagg
     2401 tagtttctac tataaaccaa atgatacaac tcataaagac caattggagt acaatgaatc
     2461 agctcctttt gaaagcaaca actatactgc cttgttaaca caatcagcgt cttggggcta
     2521 tgttgattat gtcaaaaatg gtggggagtt agaattagca ccggagagtc caaaaagaat
     2581 tattttagga acttttgaga ataaggttga ggataaaaca attcatcttt tggaaagaga
     2641 tgcagcgaat aatccatatt ttgccatttc tccaaataaa gatggaaata gggacgaaat
     2701 cactccccag gcaactttct taagaaatgt taaggatatt tctgctcaag ttctagatca
     2761 aaatggaaat gttatttggc aaagtaaggt tttaccatct tatcgtaaaa atttccataa
     2821 taatccaaag caaagtgatg gtcattatcg tatggatgct cttcagtgga gtggtttaga
     2881 taaggatggc aaagttgtag cagatggttt ttatacttat cgcttacgtt acacaccagt
     2941 agcagaagga gcaaatagtc aggagtcaga ctttaaagtt caagtaagta ctaagtcacc
     3001 aaatcttcct tcacgagctc agtttgatga aactaatcga acattaagct tagccatgcc
     3061 taaggaaagt agttatgttc ctacatatcg tttacaatta gttttatctc atgttgtaaa
     3121 agatgaagaa tatggggatg agacttctta ccattatttc catatagatc aagaaggtaa
     3181 agtgacactt cctaaaacgg ttaagatagg agagagtgag gttgcggtag accctaaggc
     3241 cttgacactt gttgtggaag ataaagctgg taattttgca acggtaaaat tgtctgacct
     3301 cttgaataag gcagtagtat cagagaaaga aaacgctata gtaatttcta acagtttcaa
     3361 atattttgat aacttgaaga aagaacctat gtttatttct aaagaaggaa aagtagta
//
LOCUS       DATRQA010000161         3410 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.161, whole genome shotgun sequence.
ACCESSION   DATRQA010000161 DATRQA010000000
VERSION     DATRQA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.161"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..243)
                     /locus_tag="VBD27_001834"
     CDS             complement(<1..243)
                     /locus_tag="VBD27_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025816.1"
                     /translation="MVNDLNNNFYLNADDTLKPIKNVLEPISYALYSIIVYFLGPQIE
                     QGIQDKAKVDYLVNATAKTTKQIPEEYFSDENKLLIA"
     gene            298..507
                     /locus_tag="VBD27_001835"
     CDS             298..507
                     /locus_tag="VBD27_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025817.1"
                     /translation="MRPKKYPYSQKNYPAPKLHNIIETDNHFLIDDKRIHYVIEDSVK
                     TKTLGDGYVEVTLSIIAKSFTKSQG"
     gene            complement(496..882)
                     /locus_tag="VBD27_001836"
     CDS             complement(496..882)
                     /locus_tag="VBD27_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025818.1"
                     /translation="MFDYFKLYTIVLETFVETKPDDAYSLFDNLSLNSEFVKFSKDLD
                     KNIVISGTLEVIDNLLDDCLIKGKRRPTKDITFYFFDGVTTTGYLYLQSLKDNNFSSR
                     LKNILKEEGIPLTPTSITRTIAKLTL"
     gene            955..1170
                     /locus_tag="VBD27_001837"
     CDS             955..1170
                     /locus_tag="VBD27_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7025819.1"
                     /translation="MTKMTLEMARAKVSMTQEEIARKIGVDRNTYASYENYKTPMRID
                     KAINFCKVVNVSIDDIIFLKQNYTSSV"
     gene            1187..1936
                     /locus_tag="VBD27_001838"
     CDS             1187..1936
                     /locus_tag="VBD27_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HEO7025820.1"
                     /translation="MNQLINITLNENQEPVVSGRDLHNVLNIKTQYTKWLERMSEYGF
                     EENVDYIAISQKRLTAQGNRTEYIDHVLKLDMAKEIAMLQRNEKSKQVRKYFIQVEKD
                     FNSPEKIMARALLMADKKITNLTMENNQLQLDLKEAQKQARYLDLIIESKGALRVTQI
                     AADYGMSANKFNKTLLEFGVQHKVNGQWILYKRHMGKGYTDSHTFDYQDKNGHTRANV
                     TTTWTQKGRLFLYELLKDNNILPLIEQEDIV"
     gene            complement(2068..2289)
                     /locus_tag="VBD27_001839"
     CDS             complement(2068..2289)
                     /locus_tag="VBD27_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025821.1"
                     /translation="MSKLSLKPNFEEKKLKTWEDLESQLKFAFDERLTTHITNINPSH
                     FEMSREEITSELKASGYEVIEQGEYLIIR"
     gene            2433..2618
                     /locus_tag="VBD27_001840"
     CDS             2433..2618
                     /locus_tag="VBD27_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7025822.1"
                     /translation="MWEKINKIMLERNLNMNKLAKLTGINKSHFSDLKSGRIKNLSWT
                     NMVKLADALDISLDEFR"
     gene            2830..2976
                     /locus_tag="VBD27_001841"
     CDS             2830..2976
                     /locus_tag="VBD27_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025823.1"
                     /translation="MAKEHYIVTHVMSDGTELDDIKGCVIPDDNPVYDIFRRINQERW
                     EAES"
     gene            3019..3339
                     /locus_tag="VBD27_001842"
     CDS             3019..3339
                     /locus_tag="VBD27_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO7025824.1"
                     /translation="MNNHFLQDKKLSLQAKGLLAEILSNKDDWRIYISELESRSTNGR
                     DAHRKAYKELQESGYIRIVKKSDGKNGVQTFAFAQDLPITDSYFAYIQDAFEKELSTS
                     GVDK"
BASE COUNT         1268 a          525 c          612 g         1005 t
ORIGIN      
        1 tgcgattaat aatttatttt catcagaaaa atattcttca ggtatttgct ttgtagtttt
       61 agcagtagca tttactaagt aatcgacttt tgctttatct tgtatgcctt gttctatttg
      121 cggacctaaa aagtagacaa taatcgaata aagggcatat gaaattggtt ctaaaacgtt
      181 tttaatgggt tttagagtat cgtcagcatt aagatagaaa ttgttattta aatcattaac
      241 catattttca cctcgacatt tttatttcaa ttataccaca gaaagaaggt ggaatagatg
      301 cgccccaaaa aatatccgta tagccaaaaa aactatccag cgccgaagct tcataacatc
      361 attgaaaccg ataatcattt cttgatagat gataaaagga ttcattatgt tattgaagat
      421 tctgtaaaga caaaaaccct tggcgatggc tatgttgaag taacactttc cataattgcc
      481 aagagtttta caaaatcaca aggttagttt ggcgatagtc cttgtgatag aagttggtgt
      541 aagagggata ccttcttctt taaggatatt ctttaaacgg ctcgaaaaat tattatcttt
      601 aagactttga agatataagt aaccagttgt tgttacgcca tcgaaaaaat agaaagtaat
      661 atcttttgtt gggcgacgtt ttccttttat caagcaatca tcaagtaaat tatcaattac
      721 ttctaaagtg cctgatatta caatgttttt atctaagtct ttagagaatt ttacaaattc
      781 actgttaaga cttagattat caaataagct ataagcgtcg tctggtttgg tttccacaaa
      841 ggtttccaag acaattgtat aaagtttgaa atagtcaaac atcacattca cctcctttct
      901 gctcacatta tagcagatta gaggtactaa aaacagatag aaaggggtgg gggaatgaca
      961 aaaatgacac tagagatggc aagagccaaa gtgtcaatga cgcaagaaga aatagctaga
     1021 aaaatcggtg tagataggaa tacatatgct agctacgaga attataaaac tccaatgcgt
     1081 atcgataaag ctattaattt ttgtaaagtt gttaacgtat caattgatga tattattttt
     1141 ttaaaacaaa actacacttc aagtgtataa cactagaaag gaaattatga atcaactaat
     1201 aaacatcacc ctaaacgaaa atcaagaacc agttgtcagt ggtagagatt tacacaatgt
     1261 acttaatata aaaactcaat atactaaatg gcttgaaagg atgtcggaat atggttttga
     1321 agaaaacgtt gactatatag ctattagtca aaaaagacta acagctcaag gtaaccgtac
     1381 agaatatata gaccacgttc tcaagctaga catggcaaaa gagattgcta tgttacagcg
     1441 taacgagaaa tcaaaacaag tccgcaaata cttcattcag gttgaaaaag acttcaatag
     1501 tcctgagaaa atcatggcga gagccttgct catggcagat aagaaaatca ctaacttaac
     1561 gatggaaaac aaccaattgc agttagactt aaaagaagct caaaagcaag cgcgttatct
     1621 tgatctaatc attgagagca agggagcttt gcgagtaaca cagattgctg ctgattatgg
     1681 tatgtctgcc aataaattca ataaaacact attagaattt ggtgtgcagc acaaagtgaa
     1741 tggacaatgg attctctata aacgacatat gggcaaaggt tacacagaca gtcacacatt
     1801 tgactatcaa gacaaaaacg gccatacaag agccaatgtg acgacgacgt ggacacaaaa
     1861 ggggcgtcta ttcctgtacg agttgttgaa agacaacaat attttgccac taattgaaca
     1921 ggaagacatt gtatagaaag gaaacatatg agaccaaaac aatatccgta tagcggaaat
     1981 aaaaaagaat ctattgcggt aacagtagat tctgaaaaat tagtcctttt acaagaacaa
     2041 atttcgattc ttgaagagag acttaactta tctaatgatt agatattctc cttgttcgat
     2101 aacttcataa ccacttgctt tcaattcaga agtaatctct tctctagaca tttcaaaatg
     2161 agacgggttt atgttagtta tgtgagttgt aagtcgctca tcaaatgcaa attttaattg
     2221 agactctaaa tcttcccaag tttttagttt tttctcttca aaatttggtt tcagacttaa
     2281 tttactcata accttcctcc tttccataat tttttgaata caacgatgag aggtcatatt
     2341 caaataaatt atatcagaaa ggaacgaaag acacaacatg ttgttatata aataaaaaat
     2401 gaacacaata tgttgatttt gggagaaata ttatgtggga aaaaatcaat aaaataatgc
     2461 ttgagagaaa tctcaatatg aataaattag caaaactaac agggattaat aaaagccact
     2521 ttagtgattt aaaaagtggg aggataaaaa acttgtcttg gactaatatg gtaaaacttg
     2581 cagatgcact tgatattagc ttagacgaat ttagataaga aaaaaagtcc gacgggaatc
     2641 ggactcaaaa caaacttaat ttacttaatt atatcacatt atgaaagaac ttaatagcat
     2701 ccaacaacta cttgttaaca attggcaacg taaatactac cagttgagcg atgttttgct
     2761 aaacagcttg gtaggtttaa caattgcaga tactcttgaa gttttagcgg ttgcgagaaa
     2821 ggaaagtata tggctaaaga acattacatt gtaactcatg tgatgtcaga tggaacagag
     2881 cttgatgata ttaaaggttg tgtcatccca gatgataatc cagtatatga catctttagg
     2941 agaattaatc aagaacgatg ggaggctgag agttgatgaa atatatattt cacgaatacg
     3001 catgaaaact atacctctat gaataatcat tttttacaag ataaaaaatt gagcttacaa
     3061 gctaaaggtc tattagctga aattctcagc aataaggatg actggcgcat ctacattagt
     3121 gagcttgaaa gcaggtcaac taatggaaga gatgctcata gaaaagcata caaggagttg
     3181 caagagtcgg gttatatacg gattgtaaaa aagagtgatg gaaaaaatgg tgttcaaaca
     3241 tttgcatttg ctcaagacct gcctatcaca gatagctatt ttgcatatat acaagatgcg
     3301 tttgaaaaag agttatccac aagcggtgtt gataagtgac ttccatttta ctgaaaatcc
     3361 aaaaattgga aaaccctttt tactgaaaat ccattttact gaaaatccaa
//
LOCUS       DATRQA010000162         3409 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.162, whole genome shotgun sequence.
ACCESSION   DATRQA010000162 DATRQA010000000
VERSION     DATRQA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.162"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            111..1334
                     /locus_tag="VBD27_001843"
     CDS             111..1334
                     /locus_tag="VBD27_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO7025825.1"
                     /translation="MTNILEVKNLTKIFGKKQKAALEMVKQGKSKTEILEKTGATVGV
                     YDASFEIKEGEIFVIMGLSGSGKSTLVRMLNRLIDPSSGNIYLDGKDIAKMNVEDLRN
                     IRRHDINMVFQNFGLFPHRTILENTEFGLEMRGVSKEERTTLAEKALDNAGLLPFKDQ
                     YPSQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDTNKQTI
                     IFISHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTVLEIDGPQVALTRMHREEVSMLMATNRRRQLLGSLTADAAIEARKKGL
                     PLSEVIDKDVVTVSKDTVITDIMPLIYDSSAPIAVTDDNDRLLGVIIRGRVIEALANV
                     QDETVVESPKETVEA"
     gene            1353..3080
                     /locus_tag="VBD27_001844"
     CDS             1353..3080
                     /locus_tag="VBD27_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEO7025826.1"
                     /translation="MENLLQHKLPVAPFVESTTNWITKTFSGLFDFIQTIGNALMDWM
                     TKTLLFINPLLFIVLITIAVFFLAKKKWQLPTFTFIGLLFIYNQGLWEQLINTFNLVL
                     VASLISIIIGVPLGIWMAKSDKVKQVVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVVFALPPTVRFTNLAIREIPLELIEASDSFGSTAKQKLFKVELPLAKNTIMAGIN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGTGFVSGLSLVILAIVLDRVSQFF
                     NSKPGEKQAKTSKVKKWVGLGALALFILAALGRIVVNMTSGNEAKGQKVKIAYVQWDS
                     EVASTNVIAEVLKSKGYDVELTPLDNAVMWQTVANGNADFTTSAWLPKTHGQYFNKYK
                     NSLDDLGPHVENVKIGLVVPKYMNVNSIEELSNQADKQITGIEPGAGIMKSAKQSLKD
                     YPNLSSWKLLSASTGAMTTTLGKAIKNKDQVVITGWSPHWMFAKYDLKYLKDPKKSFG
                     GEEHINTIARKNLKKDMPKVYKIIDKFKWTKEDMESIMLDMDKGMEPAKAAQKWIKNH
                     KKEVSEWTK"
BASE COUNT         1148 a          542 c          673 g         1046 t
ORIGIN      
        1 atttttttga gtaaaaaagt gactaccctt ttacgataac ttaacgtcac tttatagata
       61 agacacataa ccttaaaaat aagatacgaa atttttagta ggagataaga atgacaaata
      121 ttttagaagt gaagaattta accaaaattt ttgggaaaaa gcaaaaagct gctcttgaaa
      181 tggttaagca aggaaaaagt aagacagaaa ttcttgaaaa aacaggagcg actgtaggtg
      241 tctatgatgc gagttttgaa ataaaagaag gcgaaatttt cgttatcatg ggactttctg
      301 gttcaggaaa atcaacttta gttcggatgt taaaccgttt gattgaccca tcatcaggaa
      361 atatctattt agatggtaaa gatattgcaa agatgaatgt agaagattta cgtaacattc
      421 gtcgtcatga tatcaacatg gttttccaaa attttggtct tttcccacat cgtacaattt
      481 tagaaaatac agagtttggg ttagagatgc gtggtgtttc taaggaagaa cgtacaacgt
      541 tagctgaaaa agctttggac aatgcaggtc tattaccgtt taaagatcag taccccagtc
      601 aattatcagg aggaatgcaa cagcgtgttg gtttagcacg tgcccttgct aacagcccta
      661 aaatccttct gatggatgag gccttctcag ctttagaccc attaattcgt cgtgaaatgc
      721 aagatgagct tcttgatctt caagacacga ataaacaaac tattattttt atcagccatg
      781 atttgaatga agcgttacgt attggtgatc gtattgctct gatgaaagat ggagaaatca
      841 tgcagattgg tacaggagaa gagattttga ctaatccggc taatgatttt gttcgtgagt
      901 ttgttgaaga cgttgatcgc tcaaaagtgt taactgctca aaatattatg ataaaacctt
      961 taacaactgt tttagaaatt gatggcccac aagttgcttt aacaagaatg catcgtgagg
     1021 aagtctcaat gttaatggca acgaatcgtc gtcgccaact tctgggatcc ctaactgcag
     1081 atgctgcaat tgaagctcgt aagaaaggtt tgccgctttc tgaagtgatt gataaagatg
     1141 ttgtaacggt ctcaaaagat acagttatta cagatatcat gcctttaatt tatgattcat
     1201 ctgctccgat agccgttact gatgataatg atagactcct aggggttatc attagaggac
     1261 gtgtgatcga agcacttgct aacgttcaag atgaaacagt agtagaatcg ccaaaagaaa
     1321 cggtagaagc ttaatagaga ggacaattca ttttggaaaa tttattacaa cataagttac
     1381 cggtcgcacc atttgtagag tcaacgacca attggattac gaaaactttt tctggtttat
     1441 ttgactttat ccagacaatc ggtaatgcat tgatggattg gatgacaaaa acattactat
     1501 tcattaatcc tttacttttt attgtactaa ttacaattgc tgtatttttc ttagctaaga
     1561 aaaaatggca attaccgaca tttactttca ttggtttgct atttatctat aaccaagggc
     1621 tgtgggaaca gttgattaat acttttaact tggttttagt agcaagttta atttctatta
     1681 ttattggtgt accactaggt atttggatgg caaaaagtga taaagttaaa caagttgtta
     1741 atccaattct tgactttatg caaactatgc cggcattcgt ttaccttatt cctgcagtag
     1801 ccttctttgg tatcggtatg gtaccaggtg tttttgcctc tgttgtattt gctctgcctc
     1861 caacagtgcg ctttactaac ttggctattc gtgaaatccc acttgaatta atagaagcat
     1921 ctgattcatt tggttcaaca gctaagcaaa aattattcaa agttgaacta ccacttgcta
     1981 agaacactat tatggcagga atcaatcaaa ctatgatgtt ggctctttct atggtggtta
     2041 caggatctat gatcggtgca cctggacttg gtcgtgaggt gttatcggcg cttcaacatg
     2101 ctgatattgg tacaggattt gttagtggtc tatcgctagt tattttagca atcgtgttag
     2161 accgtgttag tcaattcttt aattccaaac cgggtgagaa gcaagctaaa acatcaaaag
     2221 ttaaaaaatg ggtaggttta ggggccctgg ctctcttcat tctagcagct cttggtcgca
     2281 ttgttgtcaa tatgacttca ggaaatgaag ccaaaggtca aaaagtgaag atagcttatg
     2341 tccaatggga ttcagaagtt gcttcaacca acgttattgc tgaagtttta aaatctaaag
     2401 gatatgatgt tgaattgaca ccacttgaca atgcggtcat gtggcaaaca gtcgccaatg
     2461 ggaatgctga ttttacaaca agtgcttggt taccaaagac ccacgggcaa tattttaata
     2521 aatacaaaaa tagtttagat gacttaggtc ctcacgttga gaacgtcaaa ataggtttag
     2581 ttgttcctaa atacatgaac gtaaatagta ttgaagaatt atcaaatcaa gcagataaac
     2641 aaattacagg tatcgaacca ggtgcaggta ttatgaagtc tgctaaacaa tcccttaaag
     2701 attatccaaa cctttcaagt tggaagttgt tatcagcatc aacaggagct atgacaacaa
     2761 ccttagggaa ggctatcaaa aacaaagatc aagtagtcat tacaggttgg tcacctcact
     2821 ggatgtttgc taagtatgat ttaaaatacc ttaaagatcc taagaaatca tttggtggtg
     2881 aagaacatat caatacaata gcacgcaaga atttgaaaaa agatatgcca aaagtatata
     2941 aaattatcga taagttcaag tggactaaag aagatatgga atctatcatg ttagatatgg
     3001 ataaaggtat ggagcctgct aaagcagctc aaaaatggat taaaaaccac aaaaaagaag
     3061 tttcagagtg gactaaatag tatagaaaaa aacacgtctc tcttagagcg tgttttttaa
     3121 tgtgacttaa gtcgttttag ctatttaata taagttttat attattttaa tacatcaaaa
     3181 gtgagagaat gaaagaaatg gcaggttata aagtattact aagctaaatt ctcaaagtaa
     3241 gttccacctc tagtagaaat ggaatttagt ctgataaaaa ccaataaaaa ccaacgaaaa
     3301 tccgtctcag actaagttcc gttggttttt ataatacttg atttcatagg ttttcagagt
     3361 taaaaatcgc aaaaaaaggg tacaagaaaa catcctcatc tgaaagcgt
//
LOCUS       DATRQA010000163         3405 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.163, whole genome shotgun sequence.
ACCESSION   DATRQA010000163 DATRQA010000000
VERSION     DATRQA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.163"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1579)
                     /locus_tag="VBD27_001845"
     CDS             complement(<1..1579)
                     /locus_tag="VBD27_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEO7025827.1"
                     /translation="MKDRLYNKFKDFDREFCQKYIKTYQSNAYQEMKASVNLMMRNTF
                     VFNDNWDMEPCSKAYCLDPLEWDNPVTDDPEWLYMLNRQTYLFKFLVVYIVEGDKSYL
                     RQMKYFMYHWIDCQFTLKPEGAVSRTIDTGIRCMSWLKVLIFLDYFGLITETKKIKLL
                     TSLREQITYMRDYYREKDSLSNWGILQTTAILACLYYYEDELNLPEIQSFAEEELLLQ
                     IKLQILDDGSQYEQSIMYHVEVLKSLMELVILAPKYYLPLEETIEKMVTYLIAMTGPD
                     YCQLAIGDSDVTDTRDILTLATLVLKSSKTKSFSFDNVNLETLLLFGKPSIYLFEEIP
                     RATIGESAYLFPDSGHVCLRDDRRYIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDA
                     GRYTYKEEQLRYDFKRSTSHSTCTLDGQSLEMIKDSWTYNSYPKCDYCQLTSKDRYHL
                     VEGQLHVQRASDIYYHKRWLLTLPQAITLVIDKVNCPGEHVLTNQYILDDQVIYENGL
                     VNDLKLVSPTTFNLEDCL"
     gene            complement(1659..2474)
                     /locus_tag="VBD27_001846"
     CDS             complement(1659..2474)
                     /locus_tag="VBD27_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7025828.1"
                     /translation="MTGSKKLAKSDYTKTALRAFYLQNGFNYSNYQGLGYANVIYPAL
                     KKYYGDDKKALAGALEENVEFYNTNLHFLPFVTSLHLAMLDNERPEEEIRGIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLATDGLVMGPILFFVAMNTILTGIKLVTGMYGYR
                     LGTSFIDKLSEQMSVISRAANIVGVTVISSLAATQVKLTIPYTFAPEKVTSTAQKIVT
                     VQGMLDKIAPALLPALYTFLMFYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            complement(2461..3327)
                     /locus_tag="VBD27_001847"
     CDS             complement(2461..3327)
                     /locus_tag="VBD27_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO7025829.1"
                     /translation="MDISILQAVLIGLWTAFCFSGMLLGLYTNRCIVLSLGVGVILGD
                     IQTALAVGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITTAGTKGKITPEAALA
                     LSTPIAVGIQFLQTATYTAFAGAPETAKKALQAGNFRGFKIAANGTIWAFAGLGFGLG
                     VLGALSTQTLTDLFALIPPVLLNGLTLAGKMLPAIGFAMILSVMAKKELIPYILLGYV
                     LAVYFGLPVLTPTANGDGVLTSVATNSVLGVPTIGVAIIATIFALLDIFRKPAAPTKE
                     TKTEGDNQDDWI"
BASE COUNT         1133 a          665 c          553 g         1054 t
ORIGIN      
        1 taaggcaatc ttctagatta aaggtcgtag gacttactaa tttcaagtcg ttaacaagcc
       61 cattttcata aatgacctga tcatctaaaa tatattgatt tgttaaaaca tgctctcctg
      121 gacaattcac cttatcaata actaaggtaa tggcctgcgg taaagttaac aaccatcgct
      181 tatggtaata gatatcagaa gctctttgga catgtagttg tccttcgact aaatggtacc
      241 tatcctttga agtcagctga caatagtcac attttggata agaattgtat gtccaagagt
      301 ccttgatcat ttctaaagat tgcccatcaa gggtacatgt tgaatgacta gtcgaacgtt
      361 taaaatcata ccttagttgt tcttctttgt aagtatatct tcctgcatca atgaaaatag
      421 gtttcttttt atcatagaga caaacactat tattatcact atgagtatga gcgctaccaa
      481 atggaccgtt cttaaaaaat atataacgcc tatcatcacg taaacacaca tgaccagaat
      541 ctggaaaaag ataagcagac tcacctattg ttgcacgcgg tatttcttca aaaagataaa
      601 ttgatggctt cccaaacaaa agtaaagttt ctaaattaac attatcaaaa gaaaatgatt
      661 ttgttttaga agatttcaac acaagtgttg ccaaagttag aatatcacga gtatcggtca
      721 catcactgtc ccctatagct agttgacaat aatccggacc agtcatagca attagatagg
      781 taaccatttt ttcaatagtt tcttctaatg gtagataata tttaggcgca aggataacta
      841 gttccattaa ggacttcaag acttctacat gatacataat tgattgttca tactggctcc
      901 cgtcatctaa aatttgaagc ttgatttgaa gtaataattc ttcttcagca aaactctgaa
      961 tttcaggtag gtttaattca tcttcataat agtataaaca cgccaatatt gctgttgttt
     1021 gtaaaattcc ccagttactt agactatctt tttcacgata atagtccctc atataagtta
     1081 tctgctcccg taaactagtt aatagtttaa tttttttagt ttctgttatt aatccaaaat
     1141 aatctaaaaa aatcaaaact ttgagccatg acatgcaacg tatcccagta tcaatagtac
     1201 gacttaccgc cccttctggc tttagtgtaa attggcaatc aatccaatgg tacataaaat
     1261 atttcatttg tctaagataa gacttatcgc cttcaacaat gtagaccacc aggaatttaa
     1321 aaagataagt ttgacgattt agcatataca accattctgg atcatctgtt actggattat
     1381 cccactccaa aggatcaaga caatatgctt tagaacaagg ttccatatcc caattatcat
     1441 taaatacaaa agtgttcctc atcatcaaat taacagaggc tttcatttct tgataggcat
     1501 tagattggta tgttttaata tacttttgac aaaattccct atcaaaatcc ttgaatttat
     1561 tatataagcg atctttcatt tttttacaaa acgtcctcgt ccgatataaa aaggactatg
     1621 tcattgatat atcataatcc ttatttatca aaattttatt aagctaaaat acctaaccag
     1681 ctaccaagga taccaataat aaccgtaagg ataactagct tataagttgt ccatttttta
     1741 tttttaataa ggtaaaacat aaggaaagtg tagagtgctg gaagtagagc tggtgcaatt
     1801 ttatcaagca taccttggac agtaacaatt ttttgcgctg tactagttac tttttctgga
     1861 gcaaaagtgt aagggattgt aagcttaact tgtgttgcag ctaaacttga gatgaccgtt
     1921 acaccaacga tattagctgc ccgtgagata actgacattt gttcactcaa tttatcaata
     1981 aaactggtcc caagacgata accatacatg ccagtaacta atttaattcc cgttaaaata
     2041 gtattcatag ccacaaagaa cagaatcggt cccatcacca aaccatctgt cgctaaagag
     2101 gctgcaatcg ttgagaacaa gggtgctaag caaaattgtg ataaagaatc accgatacct
     2161 gcaagaggac ccataagcgc catcttaata ccgcgaattt cttcttctgg ccgctcatta
     2221 tcaagcattg ctaaatgtaa actagttaca aaaggtaaga aatgaaggtt tgtattgtaa
     2281 aactccacat tttcttctag agctcccgct aaagcttttt tgtcatctcc ataatatttt
     2341 ttgagagctg gatatatgac attagcataa ccaagacctt gatagttact gtaattaaag
     2401 ccattttgta aataaaaggc acgtaaagct gttttagtgt aatcactttt tgctaatttt
     2461 ttagatccag tcatcttggt tgtctccttc tgtcttagtt tctttagtag gtgcagctgg
     2521 ttttctgaaa atatcgagca aagcaaatat tgttgcaatt atagctaccc cgatagttgg
     2581 aaccccaagc acactgttag tagccactga tgttaaaact ccgtctccat ttgctgttgg
     2641 agttagaact ggcaaaccaa aataaacagc tagaacatat ccaagtagga tataaggaat
     2701 gagttctttt ttagccatta cagagagaat catcgcaaaa ccaattgcag gaagcatttt
     2761 cccagctaag gttagaccat taagcaaaac agggggaatg agagcaaata aatctgtcaa
     2821 agtttgcgtt gaaagagcac ccagtactcc taaaccaaat cccaatccag cgaatgccca
     2881 aattgtacca tttgctgcaa ttttaaatcc tcggaaatta cctgcttgca aagctttttt
     2941 agctgtttca ggcgcacctg caaaagcagt ataagttgct gtttgaagaa attgaatacc
     3001 aactgcaatt ggggttgata atgcaagagc agcttccggt gtgatttttc ctttagttcc
     3061 agctgttgta atagccatca aagttccaaa aatcccagga ccgattgggt ttggaggaac
     3121 tgtccctccc gctcccacac caaatcccat gtaagccagt tcagatattg ctccaactgc
     3181 caaagctgtt tgaatatctc ctaaaataac accaacgcct agagagagaa cgatacaacg
     3241 atttgtatag aggccaagca acatgccact aaaacagaaa gccgtccaca gtccaattaa
     3301 gacagcctga agaatactaa tatccatatt tttctccttt atatgatagt gagtttgtcc
     3361 aactaaatat aatctaaaat attaacttcc acagcaccat cgtta
//
LOCUS       DATRQA010000164         3396 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.164, whole genome shotgun sequence.
ACCESSION   DATRQA010000164 DATRQA010000000
VERSION     DATRQA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.164"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            169..426
                     /locus_tag="VBD27_001848"
     CDS             169..426
                     /locus_tag="VBD27_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025830.1"
                     /translation="MNPNYTNHGIKKMHYHYDDLPNDQLYRLELPNGQCGYDFISKIN
                     FNTKSKPGDCDFTRPELIRRGYRWVFDCGFAIPVEKGEQTQ"
     gene            423..596
                     /locus_tag="VBD27_001849"
     CDS             423..596
                     /locus_tag="VBD27_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEO7025831.1"
                     /translation="MKIKLFYQKHKESLDDFEYRINQFTLSVSVIDIKFSEATYGNYE
                     DMSNTTSLLVLYR"
     gene            754..1311
                     /locus_tag="VBD27_001850"
     CDS             754..1311
                     /locus_tag="VBD27_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025832.1"
                     /translation="MRTFSDTPKTFTFHYTFKDFDTAQVACHAILGYMTGSYEQPVID
                     ATYHNDDQGGHANQLVLEYAEDRKLNKVFKRICDSFKDYYNQPEDMTDEELDDVAQET
                     ELLKEVESTDKKRVVPLFDNSQEKADKQDIFMAFISDHNQLAEHVSMNYKKMTQEDLG
                     AVLESISQGFNYLYDMAIEGELLVK"
     gene            1384..1872
                     /locus_tag="VBD27_001851"
     CDS             1384..1872
                     /locus_tag="VBD27_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025833.1"
                     /translation="MLELAIESIIKPMKKQEKTRVTGTINDQSITIDLDNLIVHYEGQ
                     EFLLETIPGTYGGKRYFFLCPDCERRCRKLFKASHAFACGTCQKLHQATLNRSKTDCQ
                     YYWQLAFKECLKVDSKAKHKHGYYSYDDFPKRPKYMRMTKYLYHWKRFHYYMDKGDKY
                     WL"
     gene            1879..2031
                     /locus_tag="VBD27_001852"
                     /pseudo
     CDS             1879..2031
                     /locus_tag="VBD27_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
     gene            2006..2383
                     /locus_tag="VBD27_001853"
     CDS             2006..2383
                     /locus_tag="VBD27_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEO7025834.1"
                     /translation="MSKKNAIRKLKEFHRWQRIANSLDLSYNERYQFDIKYHPTRREH
                     LEISRECALEELDAIKYAINQLSKIDYRKILIECYLISEKKTQQDIIAELNKSQSWYY
                     ETKKRALLEFAQSYRNGVLITTA"
     gene            2452..2739
                     /locus_tag="VBD27_001854"
     CDS             2452..2739
                     /locus_tag="VBD27_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO7025835.1"
                     /translation="MHALEKNTQVNFKTNSDLLEKAKAIIAAQNLDMTASFNLFLENI
                     VQNKALPFETDTDKERAELLAGLRAEIAKSFDDLEHGRVYNASEVRANLGI"
     gene            2729..3076
                     /locus_tag="VBD27_001855"
     CDS             2729..3076
                     /locus_tag="VBD27_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEO7025836.1"
                     /translation="MVSDNKTHSLIIPETVQEQLQEIKSYIETTYFSEQAGANTVNNI
                     LYGLERLEFFPEAGFNADDRVGETIYPPHNTRCIVLGDYLAFYHILEDRKAVFVSDII
                     HSKQDYIKLFKKK"
BASE COUNT         1205 a          593 c          640 g          958 t
ORIGIN      
        1 taatagtgcg cgtgcaaaaa aagaaagtct tttggtcaaa ttaatgctac taaaaacaca
       61 aaatgtcaaa aacccttgat acaactggct ttagccctgt gttttttgct gtgctatttg
      121 attttttaaa tgttaataac acacatttta gagagaaaag agggacatat gaatcctaac
      181 tatacaaatc atggcataaa gaaaatgcat taccattatg atgacctgcc aaacgaccag
      241 ctataccgat tggagcttcc caatggtcaa tgcggttatg acttcatcag caaaatcaat
      301 ttcaacacca agtctaaacc tggtgattgt gattttacaa gaccagaact catacgccgt
      361 ggttatcgct gggtctttga ctgtggcttt gctatacccg ttgagaaagg agaacaaaca
      421 caatgaaaat caagctattt tatcaaaaac acaaagaatc actggatgat tttgaatatc
      481 gaatcaatca atttacccta tcggtatctg tgatagacat taaattttca gaagcaactt
      541 atggcaatta tgaagacatg agtaacacaa cttctttatt ggtcttgtac aggtaactaa
      601 aattacacac acgaggcggt aacaccgtca ctatacaagg agactgcacc ctttataatg
      661 agttggtcaa atatcttcta tctgggcaac aaccaaactg ggtagccatc atcaatttat
      721 cagacattat agcaatcaca aaggagaaat aacatgagaa cattttcaga cacaccaaaa
      781 acatttacat tccactatac ttttaaagac tttgatactg cgcaagttgc ctgtcatgcc
      841 attctagggt atatgactgg gagctatgag caaccagtga ttgacgcaac ttatcacaat
      901 gatgatcaag gtggtcatgc taaccagtta gtacttgaat atgccgaaga cagaaaacta
      961 aataaggtct tcaagcgtat ctgtgacagc tttaaggact attacaacca acctgaggat
     1021 atgacggatg aagaacttga tgacgtagct caagaaactg agttactaaa ggaggttgaa
     1081 agtactgaca agaagcgcgt ggttccttta tttgacaata gccaagagaa agcggacaaa
     1141 caagacatat tcatggcttt catctcagac cacaaccaac tcgccgaaca cgtttccatg
     1201 aattataaaa agatgactca agaggattta ggagctgtac ttgaatctat cagccaaggc
     1261 tttaattatt tgtatgatat ggctattgag ggtgagttac tggttaaata aacaatcaga
     1321 gggatttccc tctttttgtc gtttatcaat agttttgggt tatttgaata ctaaggagaa
     1381 aagatgttag aactagctat tgagagtatc attaaaccaa tgaagaaaca ggaaaagact
     1441 agagttacag gaacaataaa tgaccaatct atcaccatag acctagataa cctaatcgtc
     1501 cattatgagg ggcaagaatt cttacttgaa acgataccag gaacttatgg tggtaagcgc
     1561 tacttcttcc tgtgtcctga ctgtgagaga cgttgtagaa agttatttaa ggcttctcat
     1621 gcctttgctt gtggtacttg tcagaagctt catcaagcga cactcaatcg aagtaagaca
     1681 gattgtcaat actattggca actagccttt aaagagtgtc tgaaagtaga ttcaaaagca
     1741 aaacacaaac atggttatta tagttatgat gactttccta agcgtccaaa gtacatgaga
     1801 atgactaaat acttatatca ttggaagaga ttccattact atatggataa gggggacaaa
     1861 tactggctat aacattcgga aaaacagata gctgttatca aagctagata cgtggatgac
     1921 ttatcatggg atgagatacc cgacaagcta ggttattcaa ggaatactgc attcaaatta
     1981 catagagaag ctttagaggt attagatgag caagaagaac gctattcgta aattaaaaga
     2041 gtttcataga tggcaacgta tcgccaatag ccttgattta agctataacg agcgttacca
     2101 gtttgatata aaatatcatc ccacgcgcag agaacacctt gaaataagcc gagaatgtgc
     2161 cttagaggag ctagacgcta tcaagtatgc tatcaaccaa ctgtctaaga tagactacag
     2221 aaagatattg attgagtgtt acttgattag tgagaaaaaa actcagcaag acatcatagc
     2281 agaacttaac aaaagtcaaa gttggtacta cgagactaag aaaagagctt tgcttgaatt
     2341 cgcccagagt tataggaatg gtgtgttaat aacaacagca taggctcttg gatatatgca
     2401 actatacagg tatagtttaa agtgttataa taactaaaag gaggtgctac tatgcacgct
     2461 ttagaaaaaa atacgcaggt caactttaaa accaacagtg atttactaga gaaagctaaa
     2521 gctattatcg cagcccaaaa ccttgacatg acagctagct ttaatttgtt tttagaaaac
     2581 attgtacaaa ataaagcctt accttttgag actgacaccg ataaagaacg agctgaacta
     2641 cttgcaggtt tacgagctga aattgctaaa agttttgacg atttagagca tgggcgggta
     2701 tacaacgcta gtgaagtgag ggctaacctt ggtatctgat aataaaaccc atagccttat
     2761 tatccctgaa accgtacaag aacaattaca ggagataaaa agctatattg aaactactta
     2821 cttttcggag caggcagggg ctaacaccgt caacaatatt ctttacgggc tagaacgtct
     2881 tgaatttttc ccagaagcag gatttaacgc agatgacaga gtaggtgaaa ctatttaccc
     2941 gccacataat actcgttgta ttgttttagg ggactaccta gctttttatc acattttaga
     3001 agacagaaaa gccgtttttg tatctgatat tatccatagc aaacaagact atatcaaact
     3061 gtttaagaaa aaataaaaac gcaagattta cagcaaacta aaaagcacct ttattgggtg
     3121 ctagttcttg cctgctgaac tcattgattg acagagttgt aaattaaatt tttgcttact
     3181 ttttgctaac tttgaaggaa attttaatgt caactaatgt tgacaaaaaa agtttaaaat
     3241 gttgatttaa agctataaag acttgtaaag caaccttaag gaagtctcaa aaatacagta
     3301 caacaaaatg ctttaattat tacattacat actttaaaag cctattttat aggctttttt
     3361 tgctatatac tttttagtga gtcattatat ttaatg
//
LOCUS       DATRQA010000165         3392 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.165, whole genome shotgun sequence.
ACCESSION   DATRQA010000165 DATRQA010000000
VERSION     DATRQA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.165"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            151..1293
                     /locus_tag="VBD27_001856"
     CDS             151..1293
                     /locus_tag="VBD27_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO7025837.1"
                     /translation="MKVVVAIDSLKGSLSSLEAGNAIKESINEVIPGADVEVHPLADG
                     GEGTVEALTLGMGGTIEIIPVKGPLGEKVHASYGIIPQRQLAIIEMAAAAGITLIATE
                     ERNPLHTTTYGVGEMINDAISKGCRHFIIGIGGSATNDGGAGMLQALGYALLDKDNQE
                     ISLGAQGLADLKSISTDKVIEELKECDFKIACDVTNPLCGAQGCSSIFGPQKGADEDM
                     ITKMDTWLSNYATLATSVSEKADATIEGTGAAGGLGFAFLAFTNATLEPGIDIILSEI
                     NIEKAISEADLVVTGEGRLDGQTVMGKAPIGVAKLAKKYGKKVVAFSGSVTEDAILCN
                     QHGIDAFFPIVRRLISLDEAMSKEVAYKNMKETATQVFRLINLYNH"
     gene            1318..2574
                     /locus_tag="VBD27_001857"
     CDS             1318..2574
                     /locus_tag="VBD27_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEO7025838.1"
                     /translation="MEATFTTTGALIGLALAILLIIKKVHPAYSLILGALVGGLIGGG
                     DLVTIVNTMVLGAQGMMSSILRILTSGILAGALIKTGSAEKIAESIIKKLGQQRAITA
                     LAIATMIICAVGVFIDIAVITVAPIALAIGKKANLSKSSILLAMIGGGKAGNIISPNP
                     NTIAASEAFKVDLTSLMVQNIIPAIAALVVTIILAKIVSKKNNDISYDSEEQVGSDLP
                     AFLPAISGPLVVICLLALRPLFGITIDPLIALPLGGLISILATGYLKETVPFVEYGLS
                     KVVGVSILLIGTGTLSGIIKASNLQFDMIHLLEFLNMPTFILAPLSGIFMGAATASTT
                     SGTTIASQTFAETLIKSGVPAVSGAAMIHAGATVLDSLPHGSFFHATGGAVNMAIKER
                     MKLISYEALIGLTSTIVAVVYYCFFG"
     gene            2675..>3392
                     /locus_tag="VBD27_001858"
     CDS             2675..>3392
                     /locus_tag="VBD27_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7025839.1"
                     /translation="MKQTKKEFILGLTIIITLTLLIMLIFLVMFNKSSNSKLKQTKDR
                     RILSTSTTRTSSSSHDQKIVEKSLEKKKMVMEQDMELMNAYRLYYDYAHLSLKEVVLA
                     YMSEYGIEKNSVAFSYKNLKTGQRDAMNDTQAMTAGSTYKLPLNMLVVDAVVKGKLSM
                     EERFDITETDYEYIGEHNNYVAAFSGAMSIDDMQKYSLVYSENTPAYALAERIGGMDS
                     AYSKFGRYGQSKGDIKNIQKN"
BASE COUNT         1069 a          512 c          712 g         1099 t
ORIGIN      
        1 ttatatataa caaatatcat atcataaaaa cgctaacatt tgttctatag tctatatttt
       61 ttgttatttg attttgttac tataattgag tgatataaaa tagtgaaaaa ataacaaaga
      121 ttcacaaaat attttcaagg aggttttcaa atgaaggtag tagtagctat tgattctttg
      181 aaagggagtc tttcttcttt agaagctggc aatgctataa aggagtcaat taatgaagta
      241 atcccagggg cggatgtgga agtacatcca ttagcagacg ggggagaagg aactgttgaa
      301 gctttgacgc taggtatggg ggggacgatc gaaataatcc ctgttaaggg gcctcttgga
      361 gaaaaagtac atgcgagtta tggtattatt ccacagcgtc agttagctat tattgaaatg
      421 gctgcagctg ctggtattac cctaattgct actgaagaga gaaatcctct ccacacaacc
      481 acttatggag ttggagaaat gataaatgat gctataagta aaggctgtag gcactttatt
      541 attggtattg gaggtagtgc tactaatgat ggtggggcag gtatgctaca agctttggga
      601 tatgcattat tagataaaga taatcaagaa atcagtttgg gagcacaagg attggcagat
      661 ttaaaatcta tctctactga taaggttatt gaggaattaa aagaatgcga ttttaaaatt
      721 gcatgtgatg taacaaatcc tttatgtggt gctcaaggtt gtagttcgat ttttggaccg
      781 caaaaaggtg ctgatgaaga tatgattaca aaaatggata cttggttaag taattatgct
      841 actttagcta cttcagttag tgaaaaagca gatgcaacta ttgaaggaac aggtgcagcg
      901 ggtgggcttg gatttgcctt tttagccttt acaaatgcca ccttagaacc tggtattgat
      961 attattctat cagaaattaa catagaaaaa gcgatttctg aagctgattt agtagttact
     1021 ggtgaaggtc gtttggatgg gcaaacggtg atgggaaaag ctccaattgg cgtagcaaaa
     1081 ttagctaaaa aatatggtaa gaaagtagtg gcattctctg gatctgtcac cgaagatgct
     1141 atactttgta atcaacatgg tattgatgct ttttttccaa tcgtacgacg ccttatcagc
     1201 ttagatgagg ctatgtctaa agaggtagct tataaaaata tgaaagaaac tgctactcaa
     1261 gtctttagat taatcaattt gtataatcat taatattgaa aggaattctg ataaattatg
     1321 gaagctacat ttacaacaac aggggcttta ataggattag cgcttgctat tttactgatt
     1381 atcaaaaaag ttcatcctgc ctatagtctc attcttggag cattagttgg tggtttgatt
     1441 ggtggcggtg atttagttac aattgttaat actatggtat taggtgcgca agggatgatg
     1501 tcatccatat tacgcatttt aacgtcaggg attttagcgg gtgctttaat taaaaccgga
     1561 tcagctgaaa aaattgcaga aagtatcatt aaaaaattag gacaacagcg agccatcaca
     1621 gctttagcca tcgcaacaat gattatttgt gcagtaggtg tctttattga tattgcagtt
     1681 attacagttg ctccgattgc attagcaata ggtaaaaaag ctaatctttc taaatctagc
     1741 atactgttag caatgattgg tggtggtaag gctgggaata ttatttcacc caatcctaat
     1801 acaattgctg cttcagaagc atttaaggtt gatttaacaa gcttaatggt acaaaatatt
     1861 attcctgcaa ttgcagcttt agtagttacg attatattag caaagattgt ttccaagaaa
     1921 aataacgata tttcttatga tagtgaggag caagtgggct ctgatctgcc agcattctta
     1981 ccggctattt ctggtccact tgtggttatc tgcttattag ctcttcgtcc tttatttgga
     2041 atcactattg atcccttgat tgcactgccg ttgggtggtc taattagtat tttagcaaca
     2101 ggttacctaa aagaaacggt accgtttgtt gaatacggtt tatcaaaagt tgttggcgtt
     2161 tcaattcttt tgattggaac aggtacactt tcaggtatta ttaaagcatc taatctacaa
     2221 tttgatatga ttcatctcct tgaattttta aatatgccga cctttatcct tgccccactc
     2281 tcaggtattt ttatgggagc agcaacagca tcaacgactt ctggaacaac tattgcttca
     2341 caaacgtttg ctgaaacctt gattaaatca ggggtaccgg cggtttcggg tgcagctatg
     2401 attcacgcag gtgcgactgt tctggattcg ctacctcatg gatccttctt ccatgcgaca
     2461 ggcggagctg tcaatatggc tataaaagaa cgtatgaaac taattagcta tgaagctttg
     2521 attggattaa ctagcacaat agttgctgtc gtttactatt gtttctttgg gtaaaatttg
     2581 gatgaatacg tgatgtttat tatcactgtt tgaatagaat tctcattgat tttttctaat
     2641 tttcttacta taataaatta gtaggagagg tgttgtgaaa caaacaaaga aagaatttat
     2701 tttaggatta accattatta ttacccttac tttattaatc atgctgattt ttttagtaat
     2761 gtttaataag tccagtaact ctaagttgaa gcagacaaaa gatagaagaa tattatctac
     2821 ttcaaccaca aggacatcca gtagctcaca tgatcaaaaa attgtagaaa agtctcttga
     2881 aaaaaagaaa atggtcatgg aacaagatat ggaattgatg aatgcctata ggctctatta
     2941 tgattatgcc catttgtcct tgaaagaggt tgttctggcc tatatgtctg aatatggcat
     3001 tgaaaaaaat agtgttgctt tttcttataa aaatttaaaa acaggacaaa gggacgctat
     3061 gaatgatact caggctatga cagcagggag cacctataag ttacctctta acatgttggt
     3121 agtagatgca gtggtcaagg gcaagttgtc catggaagaa cgctttgata ttaccgagac
     3181 tgactatgaa tatattggtg aacataataa ttatgttgct gctttctcgg gggcaatgtc
     3241 tattgatgat atgcaaaaat actctttagt ttattcagaa aatacacccg cctatgcttt
     3301 agctgagcgt attggtggca tggatagtgc ttattcaaag tttggtcgtt atggccaatc
     3361 taaaggagac atcaaaaata tacaaaaaaa cg
//
LOCUS       DATRQA010000166         3365 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.166, whole genome shotgun sequence.
ACCESSION   DATRQA010000166 DATRQA010000000
VERSION     DATRQA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.166"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..759)
                     /locus_tag="VBD27_001859"
     CDS             complement(<1..759)
                     /locus_tag="VBD27_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7025840.1"
                     /translation="MEKELGKTLRRLRKGKKVSISSLADEHLSKSQISRFERGESEIT
                     CSLLLNILDKLNITIDEFVSIHSKAHTHFFILLNRVRKYCAEKNVTKLVALLEDHNHK
                     DYEKIMIKALIFSIDQSIEPNQEELARLTDYLFTVEQWGYYEIILLGNCSRLINYNTL
                     FLLTKEMVNSFAYSEQNKTNKILVTHLAINCLIISIDHSYFEHSHYLIDKVRSLLQDE
                     VNFYEKTVFLYVTGYYHLKLGDTSSGKEDMRKALQ"
     gene            847..915
                     /gene="shp2"
                     /locus_tag="VBD27_001860"
     CDS             847..915
                     /gene="shp2"
                     /locus_tag="VBD27_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEO7025841.1"
                     /translation="MKKINKALLFTLIMDILIIVGG"
     gene            976..2565
                     /locus_tag="VBD27_001861"
     CDS             976..2565
                     /locus_tag="VBD27_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEO7025842.1"
                     /translation="MKIKKIISGFAAALIISSLSTINYEVKADDTTSEYHYISKQNNE
                     KQLISYIKEQHRLLNQFVVDNVNSFTQLNANPTIEQLNRAITLFKQKDEQLFNQVKAG
                     HLSPSNYNAIVNQRNVINQTVQNLIDQNHNKIQTSQNKAAQLVGQRNQVVNKIQAILA
                     TVNYNSVNSIQEAENLFHSLRNQIEPLVAEVNNYKAAMAILQQEVDALSTAAIETETS
                     KLATLKVSENTSVPANKVEEKTTQSEASGNKQEVTKSEEKQATSDAKASQPESANIAD
                     YDSLKEVLRNNISNQVPHISVQMEFKTQEQVDEYQKNLGSIIREIGDTLGTATEFNAK
                     SNISTYTLGGQIQRIIVKSDITITYTLKGDMVGLHKEYKQFVDSFVKENITNKNITSD
                     YEKAKVIHDHLVNNYTYATEELATTRETASGISIHAPEALYKDKRGVCQAFAVMFKDM
                     AAAGLSVWYVTGQAGGGNHAWNIVTINGVKYYVDTTWDNNIKSNKYFLVGKTIMDADH
                     LLDSQYNALAKDIPADRHQGA"
     gene            2601..2777
                     /locus_tag="VBD27_001862"
     CDS             2601..2777
                     /locus_tag="VBD27_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025843.1"
                     /translation="MSKKTDSNYLHSNCSDKKIWSDEAPLQQETIPKDRDCVIKPVIT
                     MTVEHVEIPLDDQS"
     gene            complement(2828..3022)
                     /locus_tag="VBD27_001863"
     CDS             complement(2828..3022)
                     /locus_tag="VBD27_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025844.1"
                     /translation="MLFLMIKPDYIRYFKVIDDMHDTMYQQTVFHVQLQAWKYTMKSI
                     DQLVVDDLNHISNEKTTLVK"
     gene            3021..3215
                     /locus_tag="VBD27_001864"
     CDS             3021..3215
                     /locus_tag="VBD27_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025845.1"
                     /translation="MIMSGQRATSTIQSDINIFHHIEAICISKSNSIGLPAIQNHLGQ
                     LIQRERLGLFFSITYSKAPT"
BASE COUNT         1201 a          600 c          531 g         1033 t
ORIGIN      
        1 ctgtaaagct ttcctcatat cttcttttcc tgaagaagta tcaccaagtt ttaaatgata
       61 gtatccagtt acatataaga aaacagtttt ttcatagaaa ttaacttcat cctgtaacaa
      121 tgatctaacc ttatcaatta agtagtggct atgctcaaag taagagtgat caatgctgat
      181 gattagacaa ttaattgcta agtgtgtaac taggatttta ttggttttat tttgttctga
      241 ataagcaaac gaattaacca tttctttggt caataaaaat aaagtattgt aattaatgag
      301 acgtgagcaa tttcctaaga gaataatttc ataatatccc cactgctcca ccgtaaaaag
      361 atagtctgtt aagcgtgcta attcttcttg attaggctct attgattgat caattgaaaa
      421 aataagagcc ttaatcataa tcttttcata gtctttatgg ttatgatcct ctaagagagc
      481 cactaattta gttacatttt tttcagcaca atattttctt actcgattta aaaggataaa
      541 aaaatgagtg tgcgccttag aatgaatcga cacaaattca tcaatagtga tgttgagttt
      601 gtctaaaata ttaagcaaaa gactacaagt aatttctgac tctcctctct caaaccttga
      661 gatttgtgac ttggataggt gctcatctgc taaagaagaa atactaactt tttttccttt
      721 acgtaatctt cttagtgttt ttcctaattc tttttccatt tttcccactt tcacaacaat
      781 ttaaaagacc ctttaaatcc attatataat acaagtacaa aatagaaaat cagaaaggag
      841 atagtcatga aaaaaattaa caaagctttg ttgttcacat taatcatgga tatcctaatt
      901 atcgttggtg gataaaactc cccacaaatg gttgttatgg aatcccattt tatttagttt
      961 ataacaggag aactaatgaa gattaaaaaa attattagtg gctttgccgc agctttaatt
     1021 atcagttcac tatcaactat taactatgag gttaaagctg atgacaccac cagtgagtat
     1081 cactatatca gtaagcaaaa taatgaaaag cagcttatta gttacatcaa ggaacaacat
     1141 cgtttgctca atcaatttgt tgttgataat gtcaactcat tcactcaact aaatgctaat
     1201 ccaactattg aacagttaaa tagagctata acattattta aacaaaaaga tgagcaatta
     1261 tttaaccagg tgaaagctgg tcatctctct cccagtaact ataatgctat cgttaatcaa
     1321 cgtaatgtca ttaaccaaac tgttcaaaat ctgattgacc aaaatcataa taagattcag
     1381 acaagtcaaa ataaagcagc tcagctcgtg gggcaacgta atcaggttgt taacaaaatt
     1441 caagctattt tagcaactgt aaactacaac tctgtgaatt ctatacaaga agctgaaaat
     1501 ttatttcatt cactcagaaa tcaaattgaa cctcttgtag ctgaagttaa taattacaaa
     1561 gctgctatgg caatccttca acaagaagta gatgccctat caacagcggc tattgaaact
     1621 gagacttcta aacttgctac tctcaaagtt agcgaaaata cttctgttcc tgcaaacaaa
     1681 gtagaagaaa aaactactca atcagaagcg tcaggcaata aacaagaagt aactaagagt
     1741 gaggaaaaac aggctacctc tgatgcaaag gcatcacagc ctgagtcagc taatattgcc
     1801 gattacgata gtttaaaaga agttttacga aataatatta gcaaccaagt accacacatc
     1861 agtgttcaaa tggagtttaa aactcaagaa caagttgacg aataccaaaa aaatctcgga
     1921 agcatcatcc gggaaattgg agatacactt ggaacagcaa ctgaattcaa tgccaaaagt
     1981 aacattagca cttatactct tggtggacaa atccaacgca ttattgtaaa aagcgacatc
     2041 acaatcacct atactcttaa aggtgacatg gtaggattac ataaagaata taaacagttt
     2101 gtagattctt ttgtcaaaga aaatattact aacaaaaata tcacaagtga ttatgaaaaa
     2161 gctaaagtaa ttcatgacca cttggttaat aattacactt acgcgactga agaactggca
     2221 accactcgtg aaactgctag tggtatcagt atccatgctc ctgaagcact ctacaaagat
     2281 aaacgtggtg tttgtcaagc ctttgcagta atgtttaaag atatggctgc tgctggctta
     2341 tcagtatggt atgtaactgg tcaagctgga ggtggaaatc acgcttggaa cattgttact
     2401 attaatggcg ttaaatatta tgttgataca acatgggata ataatataaa aagcaataaa
     2461 tatttccttg ttggtaaaac aataatggat gctgatcatc ttttggatag tcaatacaat
     2521 gcattagcta aagatattcc agctgatcgc catcaaggtg cataactcaa aaattgtatt
     2581 gagcctggaa aggatatgaa atgtctaaaa agactgattc aaattatcta cattccaatt
     2641 gttctgacaa gaaaatttgg agcgatgagg cgcctctgca acaggagact atccctaaag
     2701 acagagattg tgttatcaaa cctgtcatta ctatgacagt tgaacatgtg gaaatcccat
     2761 tagatgacca atcttagctc tctcctaaaa taaaaaggaa cactgccatt tttgacagtg
     2821 ttccttttta tttaaccaaa gtggttttct cattactgat atgattcaaa tcatctacaa
     2881 ctaattgatc aatcgatttc atggtgtatt tccaagcttg cagttgaaca tgaaaaacag
     2941 tctgttggta catggtatca tgcatatcgt caatgacttt gaaatacctt atataatctg
     3001 gtttaatcat taggaacagc atgatcatga gtggtcaaag agctaccagc accatccaga
     3061 gcgatataaa tatctttcat catatagaag ctatttgtat aagtaaatct aacagtattg
     3121 ggctccccgc aatacaaaat cacttgggac agctgatcca aagagaacgt ctgggtcttt
     3181 tcttctcgat aacctattcc aaagctccca cttgaagtac ttactgaatc tgttgcaaca
     3241 gaaaaacaat ccctactact agaataatag cacctctaac taataaatga ctcattccaa
     3301 cattcacatc ttttaacatc atgttccttt cattattttt tatttaggaa ttcgtcatac
     3361 tatat
//
LOCUS       DATRQA010000167         3350 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.167, whole genome shotgun sequence.
ACCESSION   DATRQA010000167 DATRQA010000000
VERSION     DATRQA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.167"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(73..783)
                     /gene="deoD"
                     /locus_tag="VBD27_001865"
     CDS             complement(73..783)
                     /gene="deoD"
                     /locus_tag="VBD27_001865"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO7025846.1"
                     /translation="MSIHIEAKQGEIADKILLPGDPLRAKFIAENFLEDAVCFNTVRN
                     MFGYTGTYKGHRVSIMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAINPDIHVRE
                     LVLAQAAATNSNIIRNDWPEFDFPQIADFKLLDKAYHIAKEMDITTHVGSVLSSDVFY
                     SNQPDRNMALGKLGVHAIEMEAAALYYLAAQHNVNALAMMTISDNLNNPEEDTSAEER
                     QTTFTDMMKVGLETLISE"
     gene            complement(767..2023)
                     /locus_tag="VBD27_001866"
     CDS             complement(767..2023)
                     /locus_tag="VBD27_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEO7025847.1"
                     /translation="MVSRLYYAVKFMIAVLFMTVMAGVGAILMHYVLMFTEWLAFGDS
                     RENTLSLLNSVTPIKRVLSLTLVSFLASLSWYYLQIKPKQITSIKQQVVFKDFSVKKS
                     PYWLHIGHAFLQLIYVGAGGPIGKEGAPREFGAINAGKISDLLALKVLDKRLLIVSGA
                     AAGLSAVYQVPLASVFFAFETLALGISLKNIVTLLVSTFGAASIAQLVISTAPLYHIS
                     KMSLNSQSLAFMFLIVLCVTPIAISFRYLNQKVTERRIKNIKILLSLPVVSLIVSVLS
                     IIYPQILGNGNALVQEVFKGTTVSLIAILVVLKMIATLSTLYAGAYGGILTPSFSIGA
                     CLGFLLASISIPLLPHLSIVTSMLVGAAIFLAITMRAPLTAVGLVISFTGQSVITIVP
                     LTIAVLFATAYDYFIRKMRSLYVNPY"
     gene            complement(2025..2834)
                     /locus_tag="VBD27_001867"
     CDS             complement(2025..2834)
                     /locus_tag="VBD27_001867"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO7025848.1"
                     /translation="MTLLEKINETRDFLQAKGVTAPEFGLILGSGLGELAEEIENPIV
                     VDYADIPNWGQSTVVGHAGKLVYGDLSGRKVLALQGRFHFYEGNTMEVVTFPVRIMRA
                     LACHSVLVTNAAGGIGYGPGTLMLIKDHINMIGTNPLIGENLEEFGPRFPDMSDAYTA
                     TYRQKAHQIAEKQNIKLEEGVYLGVSGPTYETPAEIRAFQTMGAQAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQRIKEDFKGLVKSLVAEL"
     gene            complement(2873..3280)
                     /gene="arsC"
                     /locus_tag="VBD27_001868"
     CDS             complement(2873..3280)
                     /gene="arsC"
                     /locus_tag="VBD27_001868"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416958.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEO7025849.1"
                     /translation="MEKVRIYHNPNCGTSRNVLAIIRHCGIEPEIIYYLKTPPSKMAL
                     VELLLEMKLSVRELLRTDVPAYEKFNLESSSVTDEEMIDAMIQDPVLINRPIVVTSKG
                     AKLCRPCEEILTILPVKMEKDFVKEDGQIIQSL"
BASE COUNT         1094 a          683 c          552 g         1021 t
ORIGIN      
        1 agaaagcatt atgataagaa tactgttcca ctaatacttt aagagcagtt gaaaaatctg
       61 tcatttctat cctcattcag aaataagtgt ttcgagccca actttcatca tatcagtaaa
      121 tgttgtttga cgctcttctg cacttgtatc ttcttctgga ttattcaagt tgtctgaaat
      181 cgtcatcata gcaagagcat taacattgtg ttgagctgca agatagtaga gagctgctgc
      241 ttccatctca atagcatgaa cacctagttt acccaaagcc atattacgat ctggttggtt
      301 tgaataaaac acgtctgaag ataatacact gcctacatga gttgtaatat ccatttcctt
      361 tgcgatgtga taagctttat ccaaaagttt aaaatcagca atttgcggaa aatcaaattc
      421 aggccaatca ttacggataa tattagaatt tgttgctgct gcttgtgcca aaacaagttc
      481 acgaacatgg atatcaggat taatagcacc tgctgttcct acgcgaatca atgttttaac
      541 cccataatca acaattagtt cgcgagcata aattgaaatt gaaggcatac ccataccagt
      601 tcccataata gaaacacgat gtcctttata ggtacctgta taaccaaaca tattgcgaac
      661 tgtattaaaa cacaccgcat cttctaaaaa attctcggca ataaatttcg cacggagtgg
      721 atctcctgga agtaaaattt tatctgcaat ttcaccttgt tttgcttcaa tatggattga
      781 catataaact cctcattttt cttataaaat aatcataggc agtagcaaaa agtactgcta
      841 tagttagggg aacaatagtt ataacagatt gaccagtaaa agatatgact aaaccgacag
      901 ctgtcagtgg cgctctcatt gtaatagcta aaaaaatagc tgcaccaacc aacatactgg
      961 ttactatcga taggtgggga agtaaaggaa tagaaatact agctaacaaa aaacctaaac
     1021 aagcaccaat ggaaaaagat ggtgtcagta taccgccata agcaccagcg taaagagtgg
     1081 ataatgttgc tatcatcttg agaacaacta aaatagcgat taagctaact gtggtccctt
     1141 taaagacttc ttgaactagg gcattaccat ttcctaaaat ctggggatag attattgata
     1201 aaacagatac tatcaatgac actactggca aacttaacaa tatttttata tttttaatac
     1261 gtctctctgt cactttttga tttaaatatc taaagctaat cgcaatcgga gttacacata
     1321 aaacgatcaa gaacataaaa gctaatgact gtgaattcag actcatcttt gaaatatgat
     1381 aaagaggggc tgtcgaaata actaattgag caatactagc tgcaccaaaa gtagagacta
     1441 gcaatgtcac aatatttttc agtgaaattc ctaaagctaa agtttcaaaa gcaaagaata
     1501 cactagctaa gggtacttga taaactgctg acaaacctgc agctgcacca ctaacgatta
     1561 ataaacgttt atccagcact ttaagtgcta ataaatccga tattttccca gcatttatgg
     1621 ctccaaactc tcgtggcgct ccttcttttc caataggacc accagctcct acataaatca
     1681 attgcaaaaa tgcgtgacct atatgtagcc agtaaggtga tttttttaca gaaaaatcct
     1741 taaaaacaac ctgttgttta atactagtta tttgctttgg cttgatttgt aagtaatacc
     1801 aactaaggga tgctaaaaag gagactaaag taagtgataa cactctttta attggtgtga
     1861 cactatttaa caaagacaga gtattttctc tactatcacc aaatgctaac cactctgtaa
     1921 acattaatac atagtgcatt aagatagctc caactcctgc cataactgtc ataaatagaa
     1981 cggctatcat aaacttgaca gcatagtata atcgagaaac cattttaaag ttcagcaact
     2041 aatgatttaa ctaatccctt gaaatcttct ttaatacgct gagtaacttc aacgacctcc
     2101 tcatgattga gctctgattg gaagccagcg gcaaagttag taattgctga aattcctaac
     2161 actttaagcc ctgagtgagc tgcaacgatc acctctggaa ccgtggacat acctaccgct
     2221 tgtgcgccca ttgtttggaa tgcacgaatt tctgcaggtg tttcataagt gggtcctgat
     2281 acacccaagt acacaccttc ttcgagtttg atattctgtt tttcagcaat ttggtgagct
     2341 ttttgtcgat atgttgctgt ataagcatcc gacatgtctg ggaaacgtgg tccaaattct
     2401 tcaaggttct cacctatgag agggttagtc ccaatcatat tgatgtggtc tttgatcagc
     2461 attaaagttc ctggtccgta tccaatccca cccgctgcat tagtcacaag cacactgtgg
     2521 caagccaatg ctctcatgat acgtactggg aaagtaacga cttccattgt atttccttca
     2581 taaaagtgaa aacgaccttg aagcgctaat accttacggc ctgataaatc cccatacact
     2641 aattttccag catgaccaac tactgttgac tgtccccaat ttgggatgtc tgcataatcc
     2701 acaacaatgg gattttcgat ttcttcagct aattctccta aaccagagcc taaaataaga
     2761 ccaaattctg gtgctgtgac gccttttgct tgcaaaaagt ctctagtctc attaattttt
     2821 tctaataatg tcatttactt acctcctaaa ctattattca ctatacaata tgctataaac
     2881 tttggataat ctgcccatct tctttaacaa aatctttttc catctttaca ggtaaaatgg
     2941 ttaaaatttc ttcacagggt cggcagagtt ttgcaccttt actcgtaaca actatgggac
     3001 gattgataag aacaggatct tgtatcatcg catcaatcat ttcttcatct gtaacgctag
     3061 aagattccaa attgaatttc tcataagcag gaacatctgt tcttagaagt tctctcacag
     3121 aaagtttcat ttctaacaac agttcaacta gtgccatctt gctaggtggt gttttcagat
     3181 aataaataat ttctggttca ataccacaat gacgaataat ggcaagtacg ttccttgatg
     3241 tcccacaatt tgggttatgg taaatcctaa ctttttccat catcttcctc tcaattttta
     3301 atgctacaag tgtcttaagg aaataaaaat ttataccaag tcctgcaaga
//
LOCUS       DATRQA010000168         3334 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.168, whole genome shotgun sequence.
ACCESSION   DATRQA010000168 DATRQA010000000
VERSION     DATRQA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.168"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..246
                     /locus_tag="VBD27_001869"
     CDS             <1..246
                     /locus_tag="VBD27_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025850.1"
                     /translation="DKNGIEVFDGDIVKCCRLFNDSLSEYVGQVKFVDFGWNIVDKAD
                     THDPFYNYKDGCPDEIREIENIGNIHENPELLERLEG"
     gene            268..345
                     /locus_tag="VBD27_001870"
                     /pseudo
     CDS             268..345
                     /locus_tag="VBD27_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            370..855
                     /locus_tag="VBD27_001871"
     CDS             370..855
                     /locus_tag="VBD27_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7025851.1"
                     /translation="MSDIKILDACCGSRLFWFDKNEEHTTFMDIRQETFDIHGKHVNV
                     KPDVLGDFRNMPFENNIFNLVVFDPPHLKYVGQKSIMKAQYGQLDKDNWKEDISKGFE
                     ECMRVLKVGGTLVFKWSDCQINVKEVLSAIPFTPLFGQRRGTTHWMTFVKFAELTGNG
                     G"
     gene            858..1181
                     /locus_tag="VBD27_001872"
     CDS             858..1181
                     /locus_tag="VBD27_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025852.1"
                     /translation="MSLIDEVKECGSESHKKWFDRFFDDLKIEEKLKQSAMKGFSGYK
                     IAIPKDDEYLARRLDSQKTIDLLKQRLGDGFDVEITNLESSYKIFGKPVVLSRDLCIY
                     WTNGG"
     gene            1183..1347
                     /locus_tag="VBD27_001873"
     CDS             1183..1347
                     /locus_tag="VBD27_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025853.1"
                     /translation="MENYLCYWEKRGQNWYFLANCEDSPTGRIFNLTGVKFNYCPFCG
                     RRLKEVTNES"
     gene            1337..1501
                     /locus_tag="VBD27_001874"
     CDS             1337..1501
                     /locus_tag="VBD27_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025854.1"
                     /translation="MKVKDLHKVLAKVDPELEVWINEKDEGVLTKAKNVMTWNKHPDK
                     FFINAETYPE"
     gene            1516..2025
                     /locus_tag="VBD27_001875"
     CDS             1516..2025
                     /locus_tag="VBD27_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEO7025855.1"
                     /translation="MNIEEAKKIVDKLSVDSDELWKIPMIPAHKVKALLDTLDQPKPE
                     VPQMIFDVIKSFDDDVDYLHQHMSRQSDEVREWLTHNEREFYEAWLAYPNITIEKEKL
                     YTVEIPNPNERQLSFVLMRQLSGNVSIKVMHRDNLDLLKTDNDLQLTESEIRKDFDWA
                     WQFREEVVE"
     gene            2022..2288
                     /locus_tag="VBD27_001876"
     CDS             2022..2288
                     /locus_tag="VBD27_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025856.1"
                     /translation="MKEKTIFISKKYANDFNNDKYNLSSGYYFRSGEKHDIAIVKYGE
                     KDYLKNTDLAYVVCDKIVDEGYVGFVYHGEYETWHFKLLNTEAN"
     gene            2676..3110
                     /locus_tag="VBD27_001877"
     CDS             2676..3110
                     /locus_tag="VBD27_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEO7025857.1"
                     /translation="MTFFPEINIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQSHGIPSKPVERLALNRVSAEQELEAIEQSVSMILDPEKRRILYEKYLSPYKNAD
                     KVIYTELCMSESFYYDTLDAALLAFAELYREGSLIVEQGVFD"
BASE COUNT         1220 a          477 c          701 g          936 t
ORIGIN      
        1 gataaaaacg gcattgaggt gtttgatggg gatatcgtta agtgttgcag actttttaac
       61 gactctttgt ctgagtacgt aggacaagta aaatttgtag attttggttg gaacatagtt
      121 gataaagcag atacacacga tccattttac aactacaaag acgggtgtcc agacgaaatt
      181 cgggaaatcg aaaatatcgg aaatatacac gaaaatccag aattactaga aaggttagaa
      241 ggatgattga agaacaagaa atacttgact gcattacgca ggacacaaaa gcaacttaaa
      301 cgtgctagcg agcaaaatca gagacagaca aagcggattg ctgattttga ctggaaatgg
      361 gggataggga tgtcagacat aaaaatttta gatgcttgtt gcggaagccg cctattttgg
      421 tttgataaaa atgaagaaca caccactttt atggatatta gacaagaaac atttgatatt
      481 cacggaaaac atgtaaatgt taaacctgat gttttgggag atttcagaaa tatgccattt
      541 gaaaataata tatttaactt agttgtcttc gacccaccac acttaaaata tgttggccaa
      601 aagtcaatta tgaaagcgca atacgggcaa cttgataaag acaattggaa agaagatatt
      661 tcaaaaggat ttgaagaatg catgagagtt ctaaaagtag gtggaacttt agtttttaaa
      721 tggtctgatt gtcagataaa tgtaaaagaa gttttatcag caattccgtt tacaccactt
      781 tttgggcagc gacgaggaac aactcactgg atgacatttg taaagtttgc agaattgact
      841 gggaatggag gatgaggatg tcattaattg atgaagtaaa agaatgcggt tcagaatcac
      901 acaaaaaatg gtttgataga ttttttgatg atttaaaaat cgaggagaag cttaaacaat
      961 ctgcgatgaa aggtttctct ggctataaaa tcgctatccc caaggacgat gaatacttag
     1021 ctagacgact tgacagtcaa aaaacaatag atttactaaa acaacggcta ggggacggtt
     1081 ttgatgttga aataacaaat cttgaatcgt cgtataaaat atttggtaaa ccagttgtgt
     1141 taagtagaga tttgtgtatt tattggacga atggaggata agatggaaaa ttatttatgt
     1201 tactgggaaa aaagaggaca aaactggtat ttcttggcta actgcgaaga ctcaccaact
     1261 ggaagaatat ttaatcttac tggtgttaaa ttcaattatt gtcctttctg tggacgtaga
     1321 ttaaaagagg tgacaaatga aagttaaaga tttacataaa gtgctagcta aagttgaccc
     1381 agaacttgaa gtgtggataa acgaaaaaga cgaaggagtt ttaactaaag ctaaaaacgt
     1441 aatgacttgg aacaagcatc ctgataagtt ttttatcaat gcagaaacat atccagaata
     1501 gaatgaggtg acgaaatgaa cattgaagaa gcgaaaaaaa tagtggacaa attgtcagta
     1561 gatagtgatg agctttggaa gattccaatg attccagccc ataaggtaaa agccttgctt
     1621 gacacacttg accaaccaaa accagaagtg ccacaaatga tatttgatgt gattaaaagc
     1681 tttgatgatg atgtagatta tttacatcaa cacatgagtc gacaatctga tgaagttaga
     1741 gagtggctaa ctcacaatga acgtgagttt tatgaagctt ggctagctta tccaaatatc
     1801 acaattgaaa aagagaagct ttatacagtt gagataccta atccgaatga aagacagtta
     1861 agttttgtgc tgatgagaca acttagcgga aatgtaagta tcaaagttat gcatagagat
     1921 aacttagact tactaaagac agataatgat ttacaactaa cagaatccga aatccgcaaa
     1981 gatttcgact gggcgtggca gtttagagaa gaggtggtgg aatgaaagaa aaaacaattt
     2041 ttatatccaa aaaatatgca aatgatttta acaatgacaa atataatttg tcctctggct
     2101 attattttag aagtggtgaa aaacatgata ttgctattgt taaatatggt gaaaaagatt
     2161 atttaaaaaa tactgattta gcatatgttg tatgcgataa aatcgttgat gaaggctatg
     2221 taggcttcgt ttatcatggt gaatatgaaa cttggcattt taaactatta aacacagaag
     2281 caaattaaag tcccacgcaa gcgcctaaga gcctgcaatg gctctgtggg tctacgagct
     2341 ggaatactcg ttaaacttac cctggaagct ttcagtaagt attcagctgc gtagcgtgga
     2401 ataatcgtta cgtagttata gagcgaaatt tttagaaagg gaaatatcct ccgacatttt
     2461 ttcataaaaa tctaaagtct gttatcgctc acagatgatt atacaagcgt aatgctgcaa
     2521 ataaagtgct gacgcaaaac taaaaattta atactcgaca atttaacaac aaaaataagt
     2581 cagcagagga aggaaaggag aacaataaaa aagcgctcgt gaaagcgcca ttcggtatat
     2641 attcgtacaa ctattatatc atacgaggag ctttcatgac gttttttcca gaaattaata
     2701 tacaaaagac taaatcaaac gctaagcgaa aactaagaga gtatccacgc tggcgtagga
     2761 tcgctaatga tgtagatact caaaaagtga cagccactta ttcctttgag cctagacaat
     2821 cacatggaat tcctagtaag ccagttgaac gcttagcact caaccgtgtg tcagctgaac
     2881 aagaattaga agcaattgag caatcagtta gtatgatact ggatccagaa aagcgcagga
     2941 ttttgtatga aaaatactta tctccttaca agaatgcaga taaggttatt tatacagaat
     3001 tatgtatgtc agagagtttt tattatgaca cgctagatgc tgcattatta gcttttgcag
     3061 agctttatag ggagggttct ttgattgtag agcaaggagt ttttgactag tttttataca
     3121 gtaatacaat agtttataca taaaaatatg tgttaatata gtattatcaa aatagcaaga
     3181 agagataatc atttaccaac aggctattta tttagtcgtc aactttaact actatcgaac
     3241 ttgctatttt gtagctaaaa ggcgagatag ggtgttgaga cgtagctcag ttgggggagc
     3301 gatatgacta taaagggtct ggaacgtacg cagg
//
LOCUS       DATRQA010000169         3224 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.169, whole genome shotgun sequence.
ACCESSION   DATRQA010000169 DATRQA010000000
VERSION     DATRQA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.169"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(300..1040)
                     /locus_tag="VBD27_001878"
     CDS             complement(300..1040)
                     /locus_tag="VBD27_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7025858.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENI
                     TFAPIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPD
                     ILLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRF
                     LEDGTPEQIFDTPQHPRLQDFLNKVLNV"
     gene            complement(1040..3223)
                     /locus_tag="VBD27_001879"
     CDS             complement(1040..3223)
                     /locus_tag="VBD27_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO7025859.1"
                     /translation="MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDS
                     DQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAAVNAVQSGQADALMAGTTVTEARKKV
                     FNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKYGYKVKT
                     FDTSDLMNNSLDSGSIYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGH
                     DNLIKEFNTAFAQMKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVS
                     DSSFAPFEYQNGKGKYTGFDMELITKIAKQQGFKLDISNPGFDAALNAVQSGQADGVI
                     AGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGTASYTWLS
                     DHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSG
                     DIGFAVKKGANPELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDEST
                     ILGLISNNYKQLLSGIGTTLSLTLISFAIAMVIGIIFGMMSVSPSNTLRTISMIFVDI
                     VRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIAEIVRGGIE
                     AVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVE
                     LFQTGKIIIARNYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK"
BASE COUNT          984 a          594 c          564 g         1082 t
ORIGIN      
        1 aactagataa gcaataatag ttgtccaaaa ccttaaatgg atgttactca ccttttccca
       61 tttaatatct aaaaagtcat ctataaagtt gacatgataa gtcatatgtt ctttaataaa
      121 gtgcaatatt ctgttaatat agtctttcat actgctatat tatcatactg aaagcccttg
      181 tgcaacatta ttcaaacatt ctagctgatg ttttgctaat tttagaagaa aacttatcct
      241 attataattt gtttgtgatc aggatataaa aaaagatgac acgaagtcat ctttttttat
      301 taaacgttta aaactttatt aaggaagtct tggagacgtg ggtgttgagg tgtatcaaag
      361 atttgctcag gtgtcccatc ttcgaggaaa cgaccaccat ctgtaaagat aacacgattg
      421 gcaacttgac gagcaaatcc catttcgtgt gtcacaatta acatagtcat accttgttca
      481 gctaaatctt tcataacatt gagaacatct ccaaccattt ctggatcaag tgcagaagtt
      541 ggttcgtcaa aaagtaagat atctggattc atcgctaagc ttcttgcgat agctacacgt
      601 tgtttttgtc ctccagataa actatctggt ttggcattag ctttatcggc taaaccaact
      661 ttttcaagta attccatccc atgcttttca gctgcttctt tagattcttt accaagttct
      721 ataggagcaa aagtgatgtt ttcaagaact gacatatgtg gaaaaagatt gaagtgttga
      781 aataccattc cgatattttc acgtgctttg tctatgtctg tttttggatt tgataactca
      841 aagccgtcca caacaacttt tccacttgtg attgattcaa gtaagttcaa tgtgcgaagg
      901 aaagttgatt ttcctgaacc agaaggtcca ataatacaaa caacatcacc ttcataaaat
      961 ttagcatcaa taccttttaa aacttcattt tgcccatacg acttgtgtaa atcttggaca
     1021 tcaattttta attctgccat tatttaagcc tcttttctaa acgttttgct agacgggtga
     1081 gaagggtaat gattacaaga tacataattg caaggatagc atacatacgg aatgattgat
     1141 agttacgtgc aattatgatt ttaccagttt ggaagagttc cacaagtccg attgctgata
     1201 caattgttgt atcctttaat gagatgacaa attggttgat aaagtttggt aacataaggc
     1261 gtactgcttg aggtaagata accttttgca tagttttgcc gtaagaaata cctaagctgc
     1321 gacttgcttc catttgacca gaaggaacag cttcaatacc accacgtaca atttcagcaa
     1381 tgtacgcacc accatttaaa gaaagagcga tagtagcagc aacgaagtca ttaattggac
     1441 tttggtgacc tgtgatgctt tcgattaaat taggaatacc ccagaaaata aaagcggcca
     1501 caatcatgag tggaatacca cggacaatat caacaaaaat cattgaaatt gtgcggagag
     1561 tattacttgg tgatacgctc atcataccaa agataatacc aataaccata gcaatcgcaa
     1621 acgagataag agttaaactt aaagtagttc caataccaga tagcaattgt ttgtagttat
     1681 tagaaattaa ccctaaaata gttgattcat ctacaggttt agcagcttta tcgtttgaag
     1741 aagtgctggc tgtggaaagg tattttttaa caagtttatc gtactcaccc gattttttga
     1801 gtgaagcaag accgttgtta aacattttaa ttaattctgg atttgcccct tttttcactg
     1861 caaatccgat atcgcctgat ttttcacctt tgataggtgt ttcaaattta cgaccttgat
     1921 taatagcgta agcaagaacg gcttcgtcat ccattagagc atcaattgaa cctgagttca
     1981 tactatcata cattgtagat gcttcatcaa atgctttaac atgatagttg tacttatctg
     2041 cgtggtctga taaccaagta tatgaggcag taccattttt agcaccaact gtttttcctt
     2101 ttaaatcttg gtatgatttg acattgcttc cttttttaac cgctaagata acgctagatg
     2161 tgtaataagg atcagaaaaa tcaaagattt tttggcgtgc ttctgtgatt gtggctcctg
     2221 caataacacc gtcagcttgc ccagattgga cagcatttaa agcggcatca aaacctggat
     2281 ttgagatatc aagtttaaaa ccttgctgtt tagcaatttt cgtgattaat tccatatcaa
     2341 aaccagtata tttcccttta ccgttttgat attcgaatgg tgcgaatgaa gaatcagaaa
     2401 caattttata acttggcttt acaggagttg ctttttcatt ggcattacct gttgctttgc
     2461 cgcttgtttt tgtagcgtct tttccaagcc atttatccat gatgtcatta taagtgccat
     2521 ctgatttcat ttgtgcaaaa gctgtgttaa attctttaat tagattatcg tgtccactac
     2581 cttttttgac agcaaatgca aagctaccaa ctgcttcgcc ttccatgtta atggcgtaag
     2641 cttttccttg atttatcgca aattgcacaa ctggttgatc gtccatagcg gcgtaaatag
     2701 aaccagaatc aaggctgtta ttcattaggt cgcttgtatc aaatgtttta actttatagc
     2761 cgtatttaga tttattttct tctaagaagc tttgagcagc tgttccattt tttacaccga
     2821 ctacttttcc ttttagttgt ttgtagtttg tgactttatt attattttta gtataaagaa
     2881 taacggaagt atcgtaataa gtatctgaga aattaaagac ttttttacgt gcttcagtaa
     2941 cagtagttcc ggccattagc gcatctgcct gtccagattg aacagcgtta actgcggcat
     3001 caaaacctgg atacgtcatg ttaacattcc agccagcacg cttagcgact tcgttaacga
     3061 tgtcaacatc gattccttta taagtttgat cagaatcttt aaattcgaat ggagcgtatg
     3121 ctgtatcaga aacaatattc acagtctctg cactgacctt tccaccaaac acaaagaata
     3181 ttgagagtaa agcaagcgta aaagctttta acttgtgctt catg
//
LOCUS       DATRQA010000170         3191 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.170, whole genome shotgun sequence.
ACCESSION   DATRQA010000170 DATRQA010000000
VERSION     DATRQA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.170"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..459
                     /locus_tag="VBD27_001880"
     CDS             <1..459
                     /locus_tag="VBD27_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEO7025860.1"
                     /translation="TGGNMIPMIGGLASILPNDTLFHYTDKYGNKKTITMKNIPLEAL
                     KISRKTINTKHVPIAIITNHKTASSAEMTFLSFKGLPNVKSFGQATAGYTTVNETFML
                     YDGARLALTTGIVSDRQGYKYENTPILPDQVTSLPLQESQSWLKSRINQN"
     gene            485..1237
                     /locus_tag="VBD27_001881"
     CDS             485..1237
                     /locus_tag="VBD27_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025861.1"
                     /translation="MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESG
                     SGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLS
                     VRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLI
                     TNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYRGNKNNSEFNKDISLTMSAILGGE"
     gene            1239..>3191
                     /locus_tag="VBD27_001882"
     CDS             1239..>3191
                     /locus_tag="VBD27_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025862.1"
                     /translation="MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVG
                     RDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVAS
                     LELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIF
                     LTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAP
                     ALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMK
                     QNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFE
                     EKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDF
                     RRLGHQLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGL
                     IIFANNKQIDNIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVG
                     NISTKQSFLKEGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQE
                     VGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSG
                     LTVLAISIIYFIIYRITSRTYYHIIER"
BASE COUNT         1074 a          492 c          549 g         1076 t
ORIGIN      
        1 actggcggca atatgatccc tatgattggg gggttagcct ctattttacc aaatgatacc
       61 ctatttcatt atactgataa gtatggaaat aagaaaacaa tcacgatgaa aaatataccg
      121 ctagaagcat taaaaatatc taggaaaaca atcaatacta agcatgttcc aatcgctatt
      181 ataactaatc ataaaactgc tagttcggca gaaatgactt ttttatcatt taaaggatta
      241 cctaatgtta aaagctttgg tcaagctaca gcaggatata cgactgttaa tgaaactttc
      301 atgctttacg acggtgctag attagcttta actacaggaa tagttagtga tcgacaagga
      361 tataaatacg agaacacccc tatcttacca gatcaagtaa ccagccttcc tttacaagag
      421 tcacagtctt ggttaaagag tagaatcaat caaaattagg gtataataaa taaaggagaa
      481 ttatatgtta ttagaaatca atcacttaga aaaagttttt cgtacacgtt tttcaaaaga
      541 agagacaagg gctctacaag acgtcgattt taaagttgaa caaggggaat tcattgctat
      601 tatgggagag tctggttcgg gaaaaacaac tctgctaaat attttagcta ctttagaaaa
      661 accgacaaat ggacaagtga ttttaaatgg ggaagatatt acgaaaatta aagaggcaaa
      721 attagcgagt tttcgtttga aaaatcttgg tttcgtcttt caggacttca accttttgga
      781 tactctctct gtaagagata atatctatct tcctttagtt cttgatcgta aacgttacaa
      841 agaaatggat catcgtttgt cagaattatc ttctcatctg agaattgatg acttattaga
      901 taagagacct ttcgagctct ctggtgggca aaagcaacgt gtagcgattg ctcgtagttt
      961 gataaccaac cctcaaattt tattagcaga tgaaccaaca gcagcgttag attaccgtaa
     1021 ttcagaagac ttgctgaatt tatttgaaac tattaacttg gatggacaaa ctattttgat
     1081 ggtaacccat tcagcaaatg ctgctagtca tgccaaacgc gttttattta tcaaagatgg
     1141 acgtattttt caccaacttt atcgtggtaa taagaataat agcgaattca ataaagatat
     1201 ttctttaaca atgtctgcca tattaggggg tgaataatat gttttatctc aaaatagcct
     1261 ggcataattt aaaacattct atagaccagt acataccatt cctcttagcc agtttattac
     1321 tttattcatt gacttgttct acgctactaa tcttaatgag tgctgttgga agagatatgg
     1381 ggacagcggc aacggttctt tttcttggag tgattgtttt gtcaatcttt gcggtagtca
     1441 tggaacatta tagctacaat atcttgatga aacagcgtag tagtgaattt ggactgtata
     1501 acattttggg gatgaataaa cgtcaagttg cgcgtgtagc tagtctagag ctgtttatta
     1561 tttatatatt tcttatttct ataggaagtc tgtttagtgc tttttttgct aaatttattt
     1621 atttaatttt tgtcaacatt attaactatc atgcactaaa tcttagttta agtttatggc
     1681 catttattat ttgtatcgtt atatttacag gtatttttct gactttagaa gttccagtta
     1741 ttcgacatgt tcatttatca tccccattaa gtctttttag aaagaaacaa cagggagaaa
     1801 aagaaccaaa aggtaatctt atacttgcaa ttttagcgtt agtagctatc gccatcgctt
     1861 atacaatggc tcttacttca ggtaaagcac ctgcattagc tgttatctat cgtttcttct
     1921 ttgcagtact tttagttatt gctggtactt atctttttta tattagtttt atgacatggt
     1981 acttaaaaag gttgcgtcaa aacaagcatt attattataa atctgagcat tttgtatcaa
     2041 cttcgcaaat gatttttcga atgaagcaaa atgcagtagg gttagcaagt atcactttat
     2101 tagctgttat ggctctagtt actattgcta caacagtctc actctattca aatacacaaa
     2161 atgttgttac cggactattt ccaaaatcag taagtttatc aatagataat tcaaaaggtg
     2221 acgcgaaaaa tatatttgaa gaaaagattt tgaagaaact aggtaagtca tctaaggaag
     2281 ctatcactta taatcagaca atgatttcga tgccagttag tcaatcaagt gaacttaata
     2341 tcacatctaa aaacgtcaaa catgttgata tcacaaaaac gggttttatg tacctaatta
     2401 ctcagaatga tttccgtcgt ttaggtcacc aattaccaaa attaaaagat aaccaagtgg
     2461 cttactttgt tcaaaaaggt gatagccgtt tgaaaaaaat aaatctattg ggaaataaat
     2521 ttgacgttgt aaaaaattta aaagaggctt atgtcccaga gactacaaat acttacaacc
     2581 cgggtttgat tatatttgca aacaacaaac aaattgacaa tattagaaaa gcatatttac
     2641 cttatactaa gaacatcaat acttttccaa aaacctttaa ggcatatttg gatttgaata
     2701 gtcaagagat aaattcaatt tctaaaaacg acataataga ggtagatggt aagtatgttg
     2761 gaaatattag cactaaacag agttttctaa aagaaggtta tcaaatgttt ggtgggttat
     2821 tatttactgg atttctgtta ggtataagct ttttgctagg tatagctcta attgtttact
     2881 ataagcaata ctcagaaggt catgaagata agagatctta tcgtatctta caagaagtag
     2941 gtatgagcaa aaaattagtt aaacggacta ttaattctca aattatgatt ttcttcttcc
     3001 aacctttagt ggtagcagtt attcactttg gagttgctat tccaatgcta aaacagatgc
     3061 ttttggtatt tggggtgctt aactcaacaa tcgtatatgt agtaagtggt ttgacagtac
     3121 ttgcgatttc tataatttac tttatcattt accgtataac tagtcgaact tattatcata
     3181 ttattgaacg c
//
LOCUS       DATRQA010000171         3161 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.171, whole genome shotgun sequence.
ACCESSION   DATRQA010000171 DATRQA010000000
VERSION     DATRQA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.171"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            174..746
                     /locus_tag="VBD27_001883"
     CDS             174..746
                     /locus_tag="VBD27_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO7025863.1"
                     /translation="MRKRSILPILAVLFVFMSIFSVYSIFHGDKAKEKASVIKQASQT
                     SQTSKKEVLQKKTYPNLNKYSNLEIHVSSTRQTMTITSSDKVIFKTIVSTGAKESPTP
                     KGTFVIEPERGDFFYNASSKEGAYYWVSFKEHGIYLFHSVPTDQQGNEIPEEAKQLGK
                     AASHGCVRMSRADAKWFYENIPQGTTVTIK"
     gene            1022..1873
                     /gene="ylqF"
                     /locus_tag="VBD27_001884"
     CDS             1022..1873
                     /gene="ylqF"
                     /locus_tag="VBD27_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEO7025864.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADPIRTKEWRDFYESQGLKTLAINSKEQSTVKKVTDIAK
                     ILMSDKIAKLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTYY
                     PDRLKERFKSINLEDESPEIIMTLTQKLGYRDDYDRFYNLFVKEVRDGKLGRYTLDIV
                     GEHDGNN"
     gene            1860..2621
                     /locus_tag="VBD27_001885"
     CDS             1860..2621
                     /locus_tag="VBD27_001885"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO7025865.1"
                     /translation="MATIKEIKAILETIVDLKDKRWQEYQTDSRAGVQKAILQRKKNI
                     QSHLDEEARLEQMLVYEKKLYIEHINLIAGIDEVGRGPLAGPVVAAAVILPPNCKIKH
                     LNDSKKIPKKKHQEIYQNILDQALAVGIGIQDSQCIDDINIYEATKHAMIDAVSHLSV
                     VPEHLLIDAMVLDLPIPQTKIIKGDANSLSIAAASIVAKVTRDKIMSDYDSKYPGFAF
                     SKNAGYGTKEHLEGLQKYGITPIHRKSFEPIKSML"
     gene            2642..>3161
                     /locus_tag="VBD27_001886"
     CDS             2642..>3161
                     /locus_tag="VBD27_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEO7025866.1"
                     /translation="MTEKEKMLAGQYYRPSAPELRKDREVALKNMQAFNNEDDSSKRN
                     VILQKWFGATGKSIHMEQRFVCDYGCNIYVGENFYANFNQTFLDVCEIRIGDNCMFGP
                     NCQLLTPLHPLDPIERNSGLEYGAPIQIGNNVWLGGGVTILPGVVLGDNVVVGAGSVV
                     TKSFENNVVIAGN"
BASE COUNT         1151 a          525 c          574 g          911 t
ORIGIN      
        1 ttacagttat cacaataacc tcgaatgaag tatcattcga ggttattttt ctactaaaaa
       61 tctcagtaaa tatttctatt tgattacaaa tgttacaaaa atatttgaca accttctttc
      121 tgaaacttat aatagatttg tccataacag agcaaaagag agagggatag tacatgagaa
      181 aacgttcaat tttgccaata ttagctgtct tatttgtttt catgagtatc tttagcgtat
      241 actctatttt tcatggtgat aaagctaaag aaaaagcaag tgtcataaaa caagcatctc
      301 aaacaagtca aacttcaaaa aaagaggtct tacaaaagaa gacttatcca aatctaaata
      361 aatattcaaa tctagaaatt catgtttcat ctacgaggca aacaatgaca ataacctcga
      421 gtgacaaggt catctttaaa acgattgttt caacaggagc aaaagagagc cctaccccaa
      481 aaggaacttt tgtcatagaa ccggaacgag gagatttttt ctataacgcc tcatcaaaag
      541 aaggagccta ttattgggtc tcctttaaag agcatggtat ttatctcttt catagcgttc
      601 cgactgatca acaaggaaat gagatcccag aagaagcaaa gcaattagga aaagccgcct
      661 cacatggctg tgtgcgtatg tcaagggcgg atgcaaaatg gttctatgaa aatattcctc
      721 aaggaacgac tgttaccatc aaatagtctt aatgtggtaa aaatcatgtt attaaaagct
      781 tggagtaaac ttccagagct ttttcgtata tcccttaaac tcaattagta aacgaaacct
      841 atcagcggaa taaatgtata aagtatttcg atatcattaa aaaaatgata gaataatagt
      901 gttaatcgaa tattttatcg acctttatat gcggttgaag aagaataaaa ctaaaagatt
      961 agacctctaa caaatagatg tagactttta aggtagtcta tacataagga gaatttattt
     1021 catggctact attcaatggt tcccaggaca catgtccaaa gctcgccgtc aagttcaaga
     1081 aaatattaag catgttgatt ttgttacaat tttagtggat gcaaggttac ctctttctag
     1141 tcaaaatcca atgctaacca aaatcgtcgg agacaaaccc aaactaatga tcctcaataa
     1201 agctgatcta gcagatccca ttcgaactaa agaatggaga gatttttatg aatctcaagg
     1261 tttaaagaca ttagctatta attctaaaga acaatcaaca gttaaaaaag taaccgacat
     1321 cgctaaaata ttaatgtccg ataaaatcgc taaactacgg gaacgtggta ttcaaaaaga
     1381 gacacttcgt acgatgatta ttggaattcc gaatgcaggc aaatcgactc taatgaatcg
     1441 cctagcaggt aaaaaaattg cagtggttgg taataaacca ggtgtcacaa aaggtcagca
     1501 atggctcaaa tctaataagg aactggaaat tttggacact cctggtatct tgtggcccaa
     1561 atttgaagat gaattagttg gattaaagct agcattaaca ggggcaatca aagatcaatt
     1621 attgcctatg gatgaggtaa caatatttgg attgaattac tttaaaacct attatcctga
     1681 tcgtctcaaa gagcgtttta aaagtattaa tttagaggat gaatctcctg aaatcatcat
     1741 gaccttaaca caaaaattag gttatcgtga cgattacgat cgcttttata atttatttgt
     1801 caaagaagtt cgcgatggta aattaggacg ctatacgtta gatatcgtcg gagaacatga
     1861 tggcaacaat taaagaaatc aaagcaattt tagagactat tgttgattta aaagacaaac
     1921 gttggcaaga atatcaaaca gattcacgcg caggtgttca aaaagcaatc cttcaacgaa
     1981 aaaaaaatat tcagtcacat ttagacgaag aagcgcgctt agaacaaatg ttggtctacg
     2041 aaaaaaaact ttacattgaa catataaacc ttattgcagg aatcgatgaa gtaggtagag
     2101 gtcctttagc aggaccagta gtagctgctg ctgttattct tccaccaaac tgtaaaataa
     2161 aacatctcaa tgactccaaa aaaattccaa agaaaaagca tcaagaaatc tatcaaaata
     2221 ttttagatca agcgttagca gttggtattg gtattcaaga cagccaatgt attgatgata
     2281 tcaacattta tgaagctaca aaacatgcta tgattgatgc agtttctcat ttatctgtag
     2341 ttcctgaaca tcttttaatt gatgctatgg ttttggatct accaattcca caaactaaaa
     2401 ttatcaaagg tgatgctaat tcactctcta ttgcagcagc ttcaattgta gctaaagtga
     2461 ccagagataa aataatgtct gattatgata gcaaatatcc tggatttgca ttttctaaaa
     2521 atgctggcta tggtactaaa gagcatttag agggtttgca aaaatacggt attactccga
     2581 tacatcgtaa aagttttgaa cctataaagt caatgttgta aataatacgg aagaggaaaa
     2641 gatgaccgaa aaagaaaaaa tgttagcagg acaatattat cgtccatcag cacctgaact
     2701 acgcaaggat agagaagttg cactaaaaaa tatgcaagcc tttaacaatg aagatgatag
     2761 tagcaaacgt aacgtcattt tacaaaaatg gtttggtgct actgggaaga gtatccatat
     2821 ggaacaacgt tttgtctgcg attatggttg taatatttat gttggtgaaa acttctacgc
     2881 caattttaac caaactttct tagatgtttg tgaaattcgt attggcgata actgtatgtt
     2941 tggcccaaac tgtcaactcc tcaccccact ccatcctcta gatccaatag aaagaaattc
     3001 aggattagaa tacggtgccc caatccaaat cgggaataat gtctggctag gtggaggagt
     3061 gactatttta ccaggtgttg tattaggaga taatgtcgtc gttggcgctg gaagtgtcgt
     3121 tacgaaatca tttgaaaata atgttgttat agcaggaaac c
//
LOCUS       DATRQA010000172         3149 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.172, whole genome shotgun sequence.
ACCESSION   DATRQA010000172 DATRQA010000000
VERSION     DATRQA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.172"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..2059
                     /locus_tag="VBD27_001887"
     CDS             <1..2059
                     /locus_tag="VBD27_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025867.1"
                     /translation="LKENTRTNFVVKGDTVLHKPTNKPFVVKGVDVESSLAGYHHNDF
                     PITQKTYREWFHLISNMGANTVRVKVPMNVAFYDALYHHNKESKRPLYLLQGIRIDSY
                     RNNASITAFNDNYRGYLKREAKGVVDILHGRKQVWNTDFGSRHYHYDLSPWVLGYVVG
                     DDWNSGTVAYTNHQEKKTQYKGRYFKTSVAANPFEVMLAQVMDELTHYETAKYGWQHL
                     ISFSNSPTTDPFHYRKPFEAQAPKYVQLNVENIQANSNVKAGMFAAYKAIDFHPRYKD
                     YLLFDKENISKEDRQKIKELSLSQGYVKLLNAYHKIPVLVTGYGYSTARGIAQKEIDK
                     RPLPINEKEQGQRLLEDYESFISSGSFGATINAWQDDWNARAWNTSFATNKHSQFLWG
                     DAQVFNQGYGLLGFKNAKHHYQVDGKRGKGEWKHPLMTSATGDDLYASSDESYLYLAI
                     KTKPEKLKEKRLLPIDITPKSGSRKMNGSKVTFSKSSDFVLSIDPNGKSELFVQERYN
                     ALKANYLRQLNGKDFYAFPPKKNSSNFEQINMVLRNTKIVEDMEKVKATERFLPTHPT
                     GLLKTGTTDRHQKTFDSQTDISFGKDFIEVRIPWQLLNFSDPSSQKIHDDYFKHYGVK
                     ELEIESIALGLGANSKENTLIKMADYRLKNWERPDTKTFLKDSYYSIKKEWSKERERT
                     YGT"
     gene            2199..>3149
                     /locus_tag="VBD27_001888"
     CDS             2199..>3149
                     /locus_tag="VBD27_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7025868.1"
                     /translation="MALFFTFLKVSQVIFLVLLIAYASFLVISLVIGGVHLYEKNERK
                     KSLTRNKEKIPISIIVPAYNESTTIVSSIDSLLHLDYEAYEIIVVDDGSSDNTSDVLK
                     EEFALMKISNTIDSIIATQTCKDVFQRQVGKVKLTLIVKENGGKGDALNMGINAANYD
                     YFLCLDADSMLQVDSLSQISKSIQVDPTVIAVGGLVQVAQGVKIEQGKVASYRLPWRI
                     IPCAQALEYDSSFLGARIFLDYLRANLIISGAFGLFKKDLVKAVGGYDTQTLGEDMEL
                     VMKLHFFCRNNNIPYRICYETDAVCWSQAPTNLGDLRKQRR"
BASE COUNT         1074 a          485 c          624 g          966 t
ORIGIN      
        1 attaaaagaa aatacaagaa ctaactttgt tgttaaaggt gatactgtac ttcacaagcc
       61 caccaataaa ccttttgttg ttaaaggagt agacgttgag tcttccttag cgggttatca
      121 tcacaacgat tttcctatta ctcaaaaaac gtatcgtgaa tggttccatt taatttccaa
      181 catgggggca aatactgtaa gagtcaaggt accgatgaat gttgcatttt acgatgcctt
      241 atatcaccac aacaaagaat caaagaggcc actgtatttg ttgcaaggaa tacgtataga
      301 ttcttatcgc aataatgctt ctataacagc ttttaatgat aattataggg ggtatttaaa
      361 acgagaagca aaaggcgttg tggatattct ccatgggcgt aagcaagtat ggaatactga
      421 ttttggtagc cgtcattatc attatgatct tagtccttgg gtacttggtt atgtcgtagg
      481 ggatgattgg aatagtggta ctgtcgctta tactaatcat caagagaaaa aaacgcaata
      541 taaaggacgt tattttaaaa cttctgtggc agctaatcca tttgaggtca tgctagctca
      601 agtaatggat gaattgacac attatgagac agctaaatat ggttggcaac atttgattag
      661 tttttcaaac tcaccaacaa cagacccttt tcattatcga aaaccatttg aggcacaggc
      721 tcctaaatac gtacaactaa atgtagaaaa tattcaagct aattcaaatg ttaaagcagg
      781 tatgtttgca gcatataaag ctattgattt ccatcctcga tacaaggatt atctattatt
      841 tgataaagag aatatcagta aagaagatag acaaaagatt aaagaacttt ctttgtcaca
      901 gggatacgtt aaactgctaa atgcttatca caaaatccct gttctagtca cgggttatgg
      961 ctattcgaca gcgagaggta ttgcccaaaa agaaattgat aaacgtcctc tgccgattaa
     1021 tgaaaaagaa caaggtcagc gtttactaga agattatgaa tcttttatat catccggtag
     1081 ttttggagcg actatcaatg catggcaaga cgattggaat gcaagggcgt ggaatacatc
     1141 tttcgccaca aataaacata gtcaattcct atggggggat gcacaagtat ttaatcaagg
     1201 ttatggttta ttaggcttta aaaacgcaaa acatcattat caagttgatg gtaaaagagg
     1261 caaaggagag tggaaacatc ctctgatgac tagtgcaaca ggagatgact tatatgctag
     1321 cagtgatgaa agctatctct accttgcgat taaaacaaaa cctgaaaaac taaaagaaaa
     1381 acgattatta ccaatagata ttacaccaaa atctggtagt agaaaaatga atggtagtaa
     1441 ggtcacattt tctaaatcta gtgactttgt attgtctatt gatccaaatg gcaagtctga
     1501 attatttgtc caagagcgct ataatgcctt aaaagcgaac tatcttcgac agcttaacgg
     1561 taaagatttt tatgctttcc caccaaagaa gaacagtagt aattttgagc agataaatat
     1621 ggtattgaga aatacaaaga ttgttgaaga catggaaaaa gtaaaagcaa cagagaggtt
     1681 cttaccaact catcctactg gtcttctcaa aacaggaaca actgataggc accaaaaaac
     1741 atttgattca caaacagata tttcgtttgg aaaggacttt atagaggtca gaattccgtg
     1801 gcagttgttg aatttttctg atccatcatc tcaaaaaatt cacgatgatt actttaaaca
     1861 ttatggtgtg aaggagttag aaattgagag cattgcttta ggattaggtg ctaatagcaa
     1921 agaaaacaca ctgataaaga tggcagatta tcgtttgaaa aattgggaga gacccgatac
     1981 caaaaccttt ttaaaagact cctattatag tattaagaaa gaatggtcta aagaaagaga
     2041 gagaacatat ggtacataat attttatatt tttacatttt gatttgctta gcaattattg
     2101 tttttaacat tcactatatc ttaagacaac gattagataa gcttagttgg cttcgtattc
     2161 ggaaaaagga tataaatatc aaataaaggg gattttctat ggcattattt tttaccttcc
     2221 taaaggtatc tcaagtcatt ttcttagtat tattgattgc ctatgccagc tttttagtga
     2281 tttcattagt cattggaggt gttcatctct atgaaaaaaa tgagcgcaaa aaaagtttaa
     2341 ctagaaataa agaaaaaatt ccaatttcaa ttattgtacc agcctataat gaatcaacaa
     2401 ccattgtgag ttcgattgat tcgttattgc accttgatta tgaagcgtat gaaattatcg
     2461 ttgtggatga cggttccagt gacaatactt cagatgttct caaggaagag tttgcattaa
     2521 tgaaaatctc gaatacgatt gattcaatta ttgctactca aacttgtaaa gatgtctttc
     2581 agagacaggt tggtaaggtt aaactgacac tcattgttaa agaaaatggt ggcaaagggg
     2641 atgccttaaa tatggggatc aatgctgcta attacgatta ttttctatgt ctagatgcag
     2701 attctatgtt gcaggtagat tctttgagtc aaattagcaa atccatacag gtagatccga
     2761 cggttatagc agtgggaggg cttgtccaag ttgcccaagg tgttaagatt gagcagggga
     2821 aagtagcatc ttatcgtttg ccttggagaa ttattccttg tgcacaagca cttgagtatg
     2881 attcatcatt tttaggagct cgaatctttt tagactattt aagagctaat ttaattattt
     2941 cgggggcttt cggtcttttt aagaaagacc ttgttaaagc ggtgggagga tatgacaccc
     3001 agactctagg agaagatatg gagttggtga tgaaattaca tttcttctgt cgtaataata
     3061 atattcctta tcgtatttgt tatgaaacag atgctgtttg ttggagtcaa gcaccaacga
     3121 atttagggga tttacgtaaa cagagacgc
//
LOCUS       DATRQA010000173         3016 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.173, whole genome shotgun sequence.
ACCESSION   DATRQA010000173 DATRQA010000000
VERSION     DATRQA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.173"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(26..2017)
                     /gene="uvrB"
                     /locus_tag="VBD27_001889"
     CDS             complement(26..2017)
                     /gene="uvrB"
                     /locus_tag="VBD27_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEO7025869.1"
                     /translation="MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKG
                     ATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEFFPASRDEHAFR
                     IEFFGDEIDRIREIESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQV
                     ELFEKEGKLIEAQRIRQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPFTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKG
                     ERTFITTLTKRMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSNGHVIMYADKITDSMQ
                     RAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVVDYSSLSKK
                     ERQAKIKALQKQMQDAAELLDFELAAQIRDVILELKAID"
     gene            complement(2078..2998)
                     /locus_tag="VBD27_001890"
     CDS             complement(2078..2998)
                     /locus_tag="VBD27_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7025870.1"
                     /translation="MNKRILNSQFAVYKNWPSWLLLLFACFMVYLFFFVGLTLGGVGI
                     VLVSSVLYKFSSYTFFEFINLFNNIYGELGTFSFSAFLLLIWVKFVEKRPFSELGFST
                     KFKRTLWSLIKGWSIGFILFSISVITAYILGGLDFHSYDVSKATIFYVVTLLPFWLIQ
                     SGTEELLTRGWLLPLINHRFHLAVAIGVSSTLFGILHLVNAHVTFLSIVSIICSGVLM
                     SLYMIKSGDIWSVAALHGAWNFSQGNLYGIAVSGQKAGASLLHFTVKENAPDWISGGA
                     FGIEGSLISIFVLLAAIIYLLWLIKTEETD"
BASE COUNT          993 a          624 c          466 g          933 t
ORIGIN      
        1 agagagaacc agatgagaga taagtttaat cgattgcttt tagttctaag ataacatctc
       61 ggatttgtgc agctagctca aagtccaata attcagctgc atcttgcatt tgcttttgaa
      121 gagctttaat tttagcttgc ctttcttttt tagataaact actgtaatcg acaacttttt
      181 caggcttatc gctatcatta cttttagtaa tagcaattaa atcacggatt tcttttttta
      241 ttgtttgtgg cacaattcca tgcttttcat tataatccat ttgtaaacga cgacggcgag
      301 cggtctcatc catagctctt tgcatagaat ctgttatttt atcagcatac ataataacat
      361 gaccattact gttacgtgct gcacgtccga ttgtttggat aagaccacgc tcattacgaa
      421 ggaaaccttc cttatcagca tctaagattg caacaaggga aacttcagga acatctattc
      481 cttcacgtaa gagattaata ccaattaaaa catcaaacac accaagtcgt agatcacgaa
      541 taatctctgt acgttctaag gttttaatat cgctgtgcat atacttaact ttaactccca
      601 tttctttgag ataatctgtt aagtcttctg ccatacgctt tgtcaatgtt gtaataaagg
      661 tacgctcacc tttttcagtc ctcaaattga tttcaccaag caagtcatcc atctgcccca
      721 tacttggtct aacttcaact tctggatcaa gaagtcctgt tggccggata atttgctcaa
      781 caactgtatc tgtttgctcc atttcatagt ctcctggcgt tgcagataca tagacaattt
      841 gatggacatg gctttcgaat tcttcacgac gaagtggacg gttatctaag gcagatggta
      901 aacgaaaacc gtaattaacc aacatttctt tacgcgaacg gtctccatta tacatccctt
      961 tgatttgccc cattgtcatg tgactctcat caatcataat caaaaaatct tctgggaaga
     1021 aatctaacaa agtaaaaggt ggctcacctt cacttctacc atccatgtga cgtgagtagt
     1081 tctcaacacc atttgtatat cccatttcac gaagcatttc aatatcatat tctgtccttt
     1141 gtcgaatacg ttgcgcttca atcaatttcc cttctttttc aaaaagttct acttgatttt
     1201 ccatttcagc ttgtattttt gaaatagctt cttccatatg ttcatcattt gtcataaagt
     1261 gtgttgctgg gaaaatagct aaatgttcca cttctcctag cacacgccct gttaacgatt
     1321 ctatttctct tatacgatca atttcatcac caaaaaattc aattcgaaag gcatgctcat
     1381 cacgactcgc aggaaaaaat tctacaacat cgccacgaac gcgaaatttt ccacgttgaa
     1441 aatcaatatc gttacgctca aattgaatat caacgagatt gttcaataac tgatcacgtg
     1501 aaatttcttg acctggccta aggctaacaa cactgtctgc atactcttta ggagaaccta
     1561 aaccgtaaat acaagaaaca gatgctacaa caataacgtc atttctttct aataacgaag
     1621 aagtcgctga gtgacgtaat ttgtcaattt cgtcattgac tgagctatct ttttcaatat
     1681 aagtatccga agaaggcaca taagcttctg gttgataata atcataatat gaaacaaagt
     1741 actctacggc attgtcagga aaaaattctt taaactctcc ataaagttga cctgctaggg
     1801 ttttattatg tgcaatcacc aaggttggct tgttaacttg ggcgataact tggctcattg
     1861 tgtaggtttt accagtacca gtagcacctt ttaaaatctg agctttttct cctccttcaa
     1921 tattgtcaac caacgtttca atagcttgag gttgatctcc tgaaggacta tatttggaaa
     1981 ctaatttaaa tcggttagta tcttttctat ctatcatttc tttacctcga ttactttatt
     2041 ttaacataga aaaaagaaaa agactgagct gcaacactca atctgtttct tcagtcttaa
     2101 ttagccatag aaggtatata attgcagcta agagaacaaa aatagaaatc aaacttcctt
     2161 ctattccgaa tgcaccacct gatatccagt ccggtgcgtt ttctttaacg gtaaaatgca
     2221 aaagagatgc tcctgctttc tgaccactta ctgcaatacc atagagatta ccttggctaa
     2281 aattccaagc tccatgtaag gctgcaacgc tccaaatatc accactttta atcatataca
     2341 aggacattaa aactcccgaa catataatac ttacaataga aagaaaagtg acatgggcat
     2401 ttaccaagtg caaaatgcca aataaagtgg atgagacccc tatggcaaca gctaaatgaa
     2461 aacgatgatt aattagagga agaagccacc ctcgtgttag caattcttcc gtaccgcttt
     2521 gaataagcca aaaaggaagg agagttacaa catagaaaat cgttgcctta gaaacatcat
     2581 agctatgaaa gtcaagtcct cccaatatat aagcagtgat gacagaaatt gaaaatagaa
     2641 taaaacctat actccagcct tttataagtg accacagagt tcttttaaac tttgtagaaa
     2701 aacccaattc tgaaaaaggt cttttttcta cgaattttac ccagataagc aaaaggaatg
     2761 ctgaaaaact aaaggttcct aattctccat agatattatt aaataaatta ataaactcaa
     2821 aaaaagtata ggaagaaaac ttatagagta cagaagacac tagaacaatt cccacaccac
     2881 ctagagttag accgacgaaa aaaaataggt ataccataaa acaagcaaat aacaataata
     2941 gccatgacgg ccagttttta tagacagcga actgactatt taaaatcctt ttattcataa
     3001 taatcctaaa tccttt
//
LOCUS       DATRQA010000174         3000 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.174, whole genome shotgun sequence.
ACCESSION   DATRQA010000174 DATRQA010000000
VERSION     DATRQA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.174"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..461
                     /locus_tag="VBD27_001891"
     CDS             <1..461
                     /locus_tag="VBD27_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HEO7025871.1"
                     /translation="SSFKVLSDMAYNIEKSKAESNGHLKIIEGSQIIDFHQKRSFMVL
                     EVNDNIDNGMQAMACQSLDESNHIVIAYSGTNAFNKKDILTDIDSVVLGKNNKLLTCP
                     SSITLREDGISLPCKNPSQITTSKLFAQKIKMNYPDATLWLTGHGKCSVR"
     gene            439..2913
                     /locus_tag="VBD27_001892"
     CDS             439..2913
                     /locus_tag="VBD27_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage infection protein"
                     /protein_id="HEO7025872.1"
                     /translation="MVNVQSGNISNYQKNLLDSATNFQNIFPALVNQYSSSITANESL
                     KKSLEASDNMFNDLMTTQTNTGKDLSSLIEQRHQDSISYEAFSASLLEMNNELLEKQL
                     SDIITQAQKDQETLSSQLNSIMGDDNDNDHNHKENSSAYLNVARQKIQELSEALKSQD
                     NIAKDQSEQLDKIVREGLASYFAKNNKDNITLLELLKSHSTNEKTLKDFKVKVADFTN
                     SLISSIPSLNLSELHLTQEEKAIQFTSSDSEIIKKVSNERTLYLNTNLLNRLFEARKN
                     RDEAKNKVNQLKLPSSSTRTGEQIVSVESNNPDYRVDTWTVNGKQTRTLDPSQNNNII
                     INSSYQGELSNSSITKPSYTITIGNQKQIVQNDDGEIQRSYLEAEATYQRTLQEVIDA
                     YNTTHELVAKYYIISDGEEPQNLFDQFLNQSVNDTMVDLVKNGITKYLMDENTADAQQ
                     KVKDVMEEVENSQDELADQMAKVTETNVRLTDTIKKQLETLQTINMKVQNITQDQSKV
                     NDSQKTTDQQLSDLKNQLDGLMTSATGVKDMSKSNSQEADQVNQIFTSFNKDVQDAKN
                     SGNKLSTDATDLMANFQKELANNGDFVASFSKVFNTAYKNGVPNDILLNFLSRPVAES
                     ASAVRATENTYRPFTWILLLEVVSLFIAYIFATQNLIKKLTDRYNVNRWLQTDFLNII
                     VISGLSLVIGLALGVISSRSLHVMPEYVPSWFLVMTLFSFLLIHSQYFFIKNFKAVGM
                     GLALFMIISFVYLSNAVGTVATVSGFPKLLKAINPLSILENQLSSYFDNVTTGFIFLI
                     LVLLVDVAFIIMNIFITLNFEAKVKE"
     gene            2922..>3000
                     /locus_tag="VBD27_001893"
     CDS             2922..>3000
                     /locus_tag="VBD27_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEO7025873.1"
                     /translation="MKLKRFLACLLILSCMSQTSVFSDGL"
BASE COUNT         1089 a          493 c          503 g          915 t
ORIGIN      
        1 atagtagttt taaagtgcta tcagatatgg catataatat tgaaaaatcc aaagcagaaa
       61 gtaatggaca ccttaaaatt atagaaggta gtcaaattat tgatttccat caaaaacgct
      121 cttttatggt attagaggtt aatgataata tcgataatgg tatgcaggct atggcatgtc
      181 agtctttgga tgagagtaat catattgtta tagcatattc aggaacgaac gcttttaata
      241 agaaagatat cttaacagat atcgattcag ttgtattggg gaagaacaat aaattgctaa
      301 cttgtccttc aagtattacc ctgagagagg atggcatttc tctgccttgt aaaaatccaa
      361 gtcaaattac aacatctaaa ctatttgcac aaaaaattaa gatgaactat cctgatgcaa
      421 ccctctggtt aacaggccat ggtaaatgtt cagtcaggta atatctcaaa ttaccaaaaa
      481 aatcttttag attctgcaac taatttccaa aatatctttc cagccctcgt taaccagtat
      541 agtagttcca ttactgctaa cgaatcactg aaaaaatctt tagaagcttc tgataacatg
      601 tttaatgatt tgatgacaac ccagacaaat actggaaaag atttatcaag cttaatagaa
      661 cagcgccatc aagatagcat ttcgtatgaa gcgttttcgg cctcattact agaaatgaat
      721 aacgagttat tagagaagca attatctgat attatcacac aagcacaaaa agaccaagaa
      781 acattatcat cacaactcaa tagtattatg ggtgatgata acgataatga tcataatcat
      841 aaagaaaact catcagctta tctaaatgtt gcaaggcaaa aaatccaaga actatctgaa
      901 gcactcaagt cacaagataa catcgctaaa gatcaaagtg aacaactaga taaaattgtt
      961 agagagggac tagcaagtta ctttgctaag aataataaag ataatattac tttattagaa
     1021 ttattgaaga gtcattctac taatgagaag actttgaaag attttaaagt taaggtagca
     1081 gatttcacaa attctctcat ttcaagcatt ccctcactaa acctttcgga gttgcactta
     1141 acccaagaag aaaaagccat tcagtttaca tcttctgatt cggaaatcat taaaaaagta
     1201 agcaatgaaa gaactctgta tttaaatact aatttattga atcgtctttt tgaagctagg
     1261 aaaaatagag atgaagccaa aaataaagtt aatcaattaa aattgccatc tagttctact
     1321 cgaactggtg agcagatagt ttctgttgaa tcaaataatc cagattacag ggtagatact
     1381 tggacggtta atggtaagca aacaagaacc ttggacccca gccaaaacaa taatattatt
     1441 atcaatagca gttatcaagg ggaattatct aactctagta ttacaaaacc aagctataca
     1501 atcactatcg ggaatcagaa acaaattgta caaaatgatg acggtgaaat tcaacggtct
     1561 tacttggagg cagaagcaac gtatcaacga actctacaag aagtaattga tgcatataac
     1621 accactcacg aattagtagc taaatactac attatttctg atggcgaaga accgcaaaat
     1681 ttatttgatc aatttttaaa tcaaagtgtt aacgatacga tggttgatct ggtcaaaaat
     1741 ggtataacta agtatttgat ggatgaaaac actgctgatg cgcaacagaa agttaaagat
     1801 gtcatggagg aagttgaaaa tagccaagat gaattggcgg atcaaatggc aaaagtaact
     1861 gagacaaatg tacgactaac agatacaatc aaaaaacagc ttgagactct tcaaacaatt
     1921 aacatgaagg tccaaaacat cacacaggat caatctaaag taaacgactc acagaaaaca
     1981 actgatcaac aattatctga tttgaagaac cagctagatg ggctaatgac ctctgcaact
     2041 ggcgtaaaag atatgtccaa atctaatagt caagaggcgg accaagttaa tcaaatattc
     2101 acatcattca ataaagatgt tcaagatgct aaaaattcgg gcaacaaact ttcgacggat
     2161 gcaacagatt taatggctaa cttccaaaaa gaattggcca ataacggtga tttcgtagct
     2221 tctttctcta aagtttttaa tactgcttat aaaaatggag tgccaaatga tattctcctt
     2281 aatttcttat cacgacctgt cgctgaatca gcgtctgcag ttagggcaac tgaaaatact
     2341 tatcgcccat tcacttggat actattatta gaagttgtta gcctatttat agcatatatc
     2401 tttgcgactc aaaaccttat taagaaattg acagataggt ataatgtcaa tcgctggctt
     2461 caaacagact ttttaaatat cattgtcatt tcaggcctct ctcttgtgat tggtttagca
     2521 ctgggagtta tctcaagcag aagtcttcat gtcatgcctg aatatgtacc atcttggttc
     2581 ctagttatga ctctgtttag ttttttatta attcatagtc agtatttctt tattaaaaat
     2641 tttaaagcag ttggtatggg tttagctttg tttatgatta ttagttttgt gtatctatca
     2701 aatgccgtag gcacagtagc gactgttagt ggatttccaa aattactaaa agctattaat
     2761 ccactatcta tccttgaaaa tcaattatca tcttattttg ataatgtaac aactggattt
     2821 attttcctta tcttagtact tcttgtggat gttgctttta ttatcatgaa tatttttatc
     2881 accttaaatt ttgaagctaa agtaaaagag tgaggttgtt gatgaagttg aaacgatttt
     2941 tagcctgtct actaatatta tcctgtatga gtcaaacatc agtattttct gatgggttaa
//
LOCUS       DATRQA010000175         2867 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.175, whole genome shotgun sequence.
ACCESSION   DATRQA010000175 DATRQA010000000
VERSION     DATRQA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.175"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(67..2346)
                     /gene="pcrA"
                     /locus_tag="VBD27_001894"
     CDS             complement(67..2346)
                     /gene="pcrA"
                     /locus_tag="VBD27_001894"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEO7025874.1"
                     /translation="MNPLIIGMNDKQAEAVQTTDGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKYVNPWNILAITFTNKAAREMRERAIALNPATQDTLIATFHSMCVRILRREADYI
                     GYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLDEIAYEKQA
                     GDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVL
                     LEENYRSTKKILQAANNVINHNKNRRPKKLWTQNDEGEQIVYHRANNEQEEAVFVAST
                     IDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIA
                     YLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQNMSLLDSSSNVMISPLKG
                     KAAQAVWDLANLILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIE
                     EFLSVTKNFDDNPEITVEGETGLDRLSRFLNDLALIADTDDSATETAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLTNANTRTLF
                     GKTSYNRPTRFIREIDDELIQHQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQAR
                     KSNSQPQVTAQLQALNANNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFP
                     GIGLKKLLASVAPISKKEN"
     gene            complement(2452..2838)
                     /locus_tag="VBD27_001895"
     CDS             complement(2452..2838)
                     /locus_tag="VBD27_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEO7025875.1"
                     /translation="MKFNLEQVKVFENYEIENWEEGQVTLTTKVVDSSLNYYGNAHGG
                     YLFTLCDQVSGLVAISTGYEAVTLQSNINYLRAGRLDDLLTVIGTCVHNGRTTKVVDV
                     HIKNQKDEMVTTAIFTMFVTGERKEK"
BASE COUNT          860 a          586 c          446 g          975 t
ORIGIN      
        1 atgatgcaaa aagactaaga atacaattct tagtctctga ttatttaagc atataataga
       61 agaaagttag ttttcctttt tactaatagg agcgacactc gctaataatt tctttaaccc
      121 aatacctgga aaatttattt ttaactcttg agtctttccg ctacctgaaa cttcaagcac
      181 cgtaccgtcc ccccattttt tatgagttgc aacatcacca atctcccatg atgtctcatg
      241 gctattgttc gcgttaagag cctggagttg ggcagttact tgaggttgtg aattactttt
      301 acgtgcctga agcgcttgtt gaagactcat tccttgacca aattgtgtag gctgttcttt
      361 tgaatatttc acaccaaatg aagaattaac tggtcgagcc aacccttgat gttgtatcag
      421 ctcatcatca atctcacgta taaaacgggt tggtctatta taactagttt taccaaataa
      481 agtacgggta ttagcattgg taaggaaaag tatttgttct gctctcgtaa tcccgacata
      541 agctaaacga cgctcttctt ctaattcatc agcatcctct attgcacgcg ataaaggaaa
      601 tactccttct tccataccga ttaagaaaac aactggaaat tctagaccct tggcagcatg
      661 taatgtcatt aaagtgactt cagccgtttc agtggcgcta tcatcagtat ctgctattaa
      721 agctaaatca tttaaaaaac gcgatagacg atctaaacca gtttcaccct ctactgtaat
      781 ctctgggtta tcgtcaaaat ttttagtcac tgataagaat tcttcaatat tttcaatacg
      841 cgcttgactt tctaatgtat tttgaacttg aagtgcttct agatatcctg tcttatccag
      901 taggttttct gtaatctccg ttacagttaa actatctagc ttactacgta gagtcaaaat
      961 cagattagct aaatcccaaa cagcttgagc agccttccct tttaagggcg atatcatgac
     1021 atttgaagag gaatcaagaa gagacatgtt ctgttcatag gcaaatgacc gtattttttc
     1081 taaagttcct ggccccaccc ctcttttagg ttcatttaca atccgctcaa aagaaatatt
     1141 atcagaagta tttgcaagga tattgagata agcaataaca tctcgaattt ctttacgact
     1201 atagaatttt gttccaccaa ccattgtata tggaatattg gattttaaaa gtgcttcctc
     1261 aatagtacga gattgcgcat tcgtacgata aagaacggca aaatctttga aattttttcc
     1321 ttgttctcga acgatgttat caatagttga cgctacaaaa acggcttctt cttgctcgtt
     1381 gttagctcta tgatatacaa tttgctcacc ttcatcattt tgagtccata attttttagg
     1441 acggcgattt ttattatgat tgatcacatt attagcagct tgaagtattt tcttagttga
     1501 tcgataattt tcttctaata atacaacttt ggcttgcgga tagtcctttt caaaatcaag
     1561 aatattttgc atatcagctc cacgccatcc ataaatggat tgatcggcat caccaacaac
     1621 acaaatattt ttgaaacgcg aagctaataa cttaactaat tggtattgag cgtggttagt
     1681 atcttgatac tcgtctacat ggatatattg atacctctgt tggtaatagg ccaaaacatc
     1741 tttattctga tcaaataatc gaagtgtcat cataatcaag tcatcgaaat ccatggcctc
     1801 acttctacgt aattcctctt gataagcttt atagcattta gcaattacct gtgtatacat
     1861 atctccggct tgcttctcat atgcaatttc atcaagaagg tcatttttag cattagagat
     1921 tgtacctaaa attgaacgtt cattccactt ttttgtgtcc aaatttaatt gcttaatgat
     1981 tcgcttcatt aaagtacgct gctcacctgg atcaacgatt gtaaagttac gattatatcc
     2041 aatataatca gcttcacgac gtaaaatacg aacacacata ctatgaaaag ttgcaatcaa
     2101 agtatcttgg gtagctggat tcaaggcgat agcacgttca cgcatttcac gcgctgcttt
     2161 attagtaaaa gtaatcgcta aaatattcca aggattaaca tatttttcat ctattaaata
     2221 agcaatacga tgagtcagaa cacgtgtttt tccagagcca gctcctgcca taatcaaaag
     2281 cggtccgtct gtcgtttgta ccgcttctgc ttgtttatca ttcattccaa ttattaaagg
     2341 attcatttct actcctaatt ttgtcaaaat tgcctctcta gtatatcatt ttttcatctt
     2401 tttcccaata gaaaaaggag ttaaacagac gctgtctaac tcctaccttt tttacttctc
     2461 ttttctctct ccagttacaa acattgtaaa aatagcagtt gtaaccattt cgtctttctg
     2521 atttttaatg tgaacatcaa cgacttttgt agtacgacca ttatgaacac aagtaccgat
     2581 gacagtaagc agatcatcta atcggcctgc cctcaaatag ttgatatttg attgtagagt
     2641 taccgcttca tatcctgttg aaatagcaac taaaccactg acttgatcac acagtgtaaa
     2701 caaataacca ccatgagcat taccataata gttgagcgaa gagtcaacga ccttcgttgt
     2761 taaggtaact tgaccttctt cccagttttc aatttcgtaa ttctcaaata cttttacttg
     2821 ttctaaattg aatttcattc tactataacc tcactttttc tatgacc
//
LOCUS       DATRQA010000176         2844 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.176, whole genome shotgun sequence.
ACCESSION   DATRQA010000176 DATRQA010000000
VERSION     DATRQA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.176"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(23..457)
                     /gene="nusB"
                     /locus_tag="VBD27_001896"
     CDS             complement(23..457)
                     /gene="nusB"
                     /locus_tag="VBD27_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO7025876.1"
                     /translation="MTSVFKDSRRDLRERAFQTLFSLETGGEFIDAAHFAYGYDKTVS
                     EDKVLEVPIFLLNLVNGVVDHKDELDTLISSHLKSGWSLERLTLVDKSLLRLGLYEIK
                     YFDETPDRVALNEIIEIAKKYSDETSAKFVNGLLSQFITNEN"
     gene            complement(450..839)
                     /locus_tag="VBD27_001897"
     CDS             complement(450..839)
                     /locus_tag="VBD27_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7025877.1"
                     /translation="MTTENLGEIVISPRVLEVITGIAATKVDGVHSLRNKAVTDSLSK
                     KSLGRGVYLKNEEDDTVAADIYVYLQYGVNVPAVSIAIQQAVKTAVYDMAEVKISSVN
                     IHVEGIVPEKTPKPDLKSLFDEDFLDD"
     gene            complement(928..1488)
                     /gene="efp"
                     /locus_tag="VBD27_001898"
     CDS             complement(928..1488)
                     /gene="efp"
                     /locus_tag="VBD27_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO7025878.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNNETYDQYEIPTVNIENE
                     LLYILENSEVKIQFYGTEVIGVQIPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(2416..2715)
                     /locus_tag="VBD27_001899"
     CDS             complement(2416..2715)
                     /locus_tag="VBD27_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HEO7025879.1"
                     /translation="MLLPVGSVVYLIDGNQKLVIVNRGAIVEQEGQEVYFDYLGGIFP
                     EGLNLEQVYYFNQEDIDEVVFEGYHDEEEERVSRLIEKWKNTEGKNLPKGKTSVE"
BASE COUNT          884 a          510 c          432 g         1018 t
ORIGIN      
        1 ggaagaatct tcttccacat ttttaatttt catttgttat aaattggctt aataaaccat
       61 tgacaaattt tgctgacgtc tcatctgaat attttttagc aatctcgata atctcattta
      121 gagctacacg atcaggcgtt tcatcaaaat atttaatttc atagaggcct aagcgtaaga
      181 gagatttatc aaccaatgtt agacgttcta agctccatcc tgattttaag tgactagaaa
      241 tcaaagtatc taattcatcc ttgtgatcta caacaccatt aacaagatta agtaaaaaga
      301 ttggaacctc taaaactttg tcttcagata ctgttttatc ataaccataa gcaaagtgag
      361 ccgcatcaat aaactctcca ccagtttcta agctaaatag tgtttgaaaa gcacgttcac
      421 gtaaatcacg tcttgaatct ttaaatacac tagtcatcta agaaatcctc atcaaacaat
      481 gacttcaagt ctggttttgg tgttttctct ggaacaatac cttcaacatg gatattgacc
      541 gaagaaattt ttacttctgc catgtcatat acagctgttt tgacagcttg ttgaatagca
      601 attgaaactg ctggtacatt tacaccatac tgcaagtaaa cataaatatc tgcagctact
      661 gtatcatctt cttcattttt aaggtagaca cctctaccta gagatttctt actaaggcta
      721 tccgttaccg ctttgttacg taacgaatga acaccatcaa ctttagttgc agcaatacct
      781 gtaattacct caagtacacg tggtgaaatg acaatttctc caagattttc agttgtcata
      841 gcttatcctt tcttttagtg tttactaagg tttttcctta gtagagtaaa ttcatcatga
      901 atctacttaa cacttttttg agagtcatta ggcgcgtgaa acgtaagttc cttctgctgt
      961 attgattact aacttttgtc cagcttcaat aaagtctgga acgttaacaa caagtcctgt
     1021 ttccatagtt gcaggttttc ctgaaccagt tacagtagca cctttgattg atggttgagt
     1081 ttcagcaact gtcaattcaa cagttgttgg aatttgaaca ccaataactt ctgttccata
     1141 gaattgaatt tttacttctg agttttcaag aatgtagagt aattcatttt caatgttaac
     1201 agttggaatt tcatattgat cgtaagtttc attattcatg aagtaagctg tatcatccat
     1261 cttataaagg tattgagcag gtacagtttc aataatagct tgctcaaatt tttcttcagg
     1321 acggtaactt gtatcaaaag ttgaaccagt acgaacatca cgcaatttca tacgcatgat
     1381 tgtgttacct ttacctggtt tgtggtggct agcttcaaga acacggatta atttcccatc
     1441 agccgtttca aaagtcatgc ctgctttaag cttacttgct tcaatcattt attaatacct
     1501 ctttgtaaaa attatttatc aactcatttt atcacaaata acctgctttc tcaaatagtt
     1561 tattgatttg gtatgtgtct gatttcttga taaataaata aaatcaccaa gagggcgtaa
     1621 ataagactga agcacttgac actttcctct tacttcagtt ttgataacag ttttaatatt
     1681 ataccgatat cttgactaag attgaaatac tgtttaggtg tttttagtga ctttttagta
     1741 aattttaata aaggtagtaa actatagttg tcttattgaa acagataaaa taaatcacct
     1801 acggagctat tcgtatgaaa atgtagttat tctcgatata agttttagat acgaacttaa
     1861 tttgctctat tgaagctggt aaaggaaatc ggggtggtgt tagtggacgt gcgctaagca
     1921 ttggtactga gcttggtgat accaatcttg cttactacat caggtagctc tctgttttag
     1981 tgccatatgc aattggaata agagctgtca ttggtattga aggagcccta gtccactgat
     2041 cattgaatta ctaatttagg aggtggaatg atgaatttta atatgtctca ttatagaaag
     2101 ttacaactat gtcgttttct aatcgtacag atacctgcgc tggctttatt agctattctt
     2161 ttatacggga ttttcctaaa gtacagtcta ttttcaaaag tattttttct tatcttttag
     2221 gggttgaaat agttatcaat tttgggaagt acataccagt ttacaccaac cacaatttgt
     2281 cgagtaaaga attgtaattt agtaaagcct ttttcgtttt cttctgataa gtgtatcatg
     2341 acatctatca atagcatatt cacactagta gcatattgat ttgtttttga gtagaattta
     2401 ctttttagat tatttctatt ctacactagt ctttcccttt ggaagattct ttccctctgt
     2461 atttttccat ttttcaatga gacgagaaac tctttcttcc tcctcatcat ggtaaccttc
     2521 aaaaacaacc tcatcaatat cttcttgatt aaaatagtaa acttgttcaa gatttaaacc
     2581 ctcaggaaaa attccaccta ggtaatcaaa ataaacttct tgtccttctt gctcaacaat
     2641 tgcacctcta tttacaataa ctaatttttg attgccatca ataagataga caacactccc
     2701 tactggtaat aacatacttc agttctcccc aatataataa tttttcccat ttaacgatgg
     2761 ttgaaactca atgaacttta taaaaaataa actgtttgaa aaaataaatc caaataaaaa
     2821 taagtttgaa aagttttcgt ccca
//
LOCUS       DATRQA010000177         2839 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.177, whole genome shotgun sequence.
ACCESSION   DATRQA010000177 DATRQA010000000
VERSION     DATRQA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.177"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..372
                     /locus_tag="VBD27_001900"
     CDS             <1..372
                     /locus_tag="VBD27_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7025880.1"
                     /translation="KVDMNTILGQKTVMIHINVAKKDMNSTSMMMKKDKMTMPMKKEM
                     TSSKINTGMMMSNNKMSANMQMSSQAKSNDKAGKKMSMMSKNLPNTGETKQQNVGVLG
                     MLSLAFATGLTALGLKKSKQR"
     gene            485..1171
                     /locus_tag="VBD27_001901"
     CDS             485..1171
                     /locus_tag="VBD27_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7025881.1"
                     /translation="MTQKLLLVDDEFEIIDINRRYLEQAGYEVSVAADGIEALKEVDE
                     NRFDLIISDIMMPKMDGYDFISEVLVREPNQPFLFITAKVSEPDKIYSLSMGADDFIS
                     KPFSPRELVLRVKNILRRIYGNHQQSEVLTIGDLVIDQKQRLVMVDCNTISLTNKSFD
                     LLWILANHLNRVFSKTELYERVWGEEFLDDTNTLNVHIHALRNDLAKFSTDNTPTIKT
                     VWGLGYKLEE"
     gene            1173..2210
                     /locus_tag="VBD27_001902"
     CDS             1173..2210
                     /locus_tag="VBD27_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7025882.1"
                     /translation="MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTI
                     IACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRRFDTKAQICSPQEFKDLETAFNQM
                     SSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNYYLN
                     TISRQTNRLNHLVEELSFITLETMSDTAEPHKEETIYLDKLLIDILSEFQLVFEKENR
                     QVMIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQDIVIDIID
                     QGYGIKDEDLASIFNRFYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILVESQYQKG
                     SKFSLVLKLQK"
     gene            complement(2224..>2839)
                     /locus_tag="VBD27_001903"
     CDS             complement(2224..>2839)
                     /locus_tag="VBD27_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO7025883.1"
                     /translation="YNNMTLLDFFVPLPVYFFYTLFIISVSFVMLDVVKNQKLNVRFS
                     DNTYVFSSHIFWKLLSVLVLKGLILSFFYLLSTFGLLIIISSFRLLLSGNLILAPVLI
                     VVSSLITTKAVIKLVQQYYSYSISTLVFYTQLESGNYEGPSKVLVASRELMNGNKLRL
                     FLLDLSFIGWHFLTIFSFGLVYIYLLPYQTTARLIFYRNITKNS"
BASE COUNT         1033 a          405 c          510 g          891 t
ORIGIN      
        1 aaagttgata tgaatactat tttaggacaa aagacagtga tgattcatat taatgtcgct
       61 aaaaaagata tgaatagcac atcaatgatg atgaaaaaag ataagatgac aatgcctatg
      121 aagaaagaaa tgacttcatc taaaataaat accggtatga tgatgtctaa caacaagatg
      181 tcagctaata tgcaaatgtc atcacaagcc aaatcaaatg ataaagctgg taaaaaaatg
      241 tctatgatgt ctaaaaattt acctaataca ggtgaaacaa agcaacaaaa tgtaggtgtg
      301 ctaggtatgc ttagtctagc ctttgcgaca ggtctaactg cgctaggcct taaaaaatct
      361 aaacaaagat agggatagta tttaattcag aacaccaaat ttgtttaaaa ttattgaggg
      421 ttatcgtggt aaaatagtgt tatactagag gtatagcact attttgactc attaggagag
      481 tagaatgaca caaaaattat tattagttga cgatgaattt gaaattattg atattaatag
      541 acgttatcta gagcaagcag gatatgaagt gtcagtagct gcggatggca tagaagcact
      601 caaagaggta gatgagaatc gttttgacct aattattagt gatattatga tgcctaaaat
      661 ggatggctat gattttatta gtgaagttct agttcgagaa cccaaccaac cttttctctt
      721 tataaccgct aaagtatctg aaccagataa aatttactct ttgagtatgg gtgcagatga
      781 ctttatttcc aagcctttca gtcctaggga attagtttta cgtgttaaaa atattctaag
      841 acgtatctat gggaatcatc aacaatctga agtattgacg attggtgatt tggttatcga
      901 ccaaaaacaa cgtttggtaa tggtagactg caatacaatt tccttaacca ataaatcatt
      961 tgacttgctt tggatattag caaatcattt gaatcgtgtt ttttcaaaaa cagagcttta
     1021 tgaacgtgtc tggggcgaag aatttttaga tgacactaat actttgaatg ttcatatcca
     1081 tgccttacgc aatgaccttg ctaaatttag tacagacaat accccaacta tcaaaacagt
     1141 ttggggatta gggtataaat tagaggaata aaatgaaatt aaaatattat attgttattg
     1201 gttaccttat ttctatgtta ataactgtag caggtgtttt ctttgggctt aatcatatgc
     1261 tgattgagac tcgaggtgtt tactacatcc tttcagttac aattattgcc tgtatcgtag
     1321 gtggtatagt taatttgttc ttattatcaa gtgtatttac atctttgaaa aagttaaaac
     1381 aaaaaatgaa ggatatttct caaagacgtt ttgatacaaa agcacaaatt tgttcaccac
     1441 aagaattcaa ggatttagag acagccttta accaaatgtc ttcagagtta gagtcaacgt
     1501 ttaaatctct aaacgagagt gaacgcgaaa aaacaatgat gattgcacaa ctttcacatg
     1561 atatcaagac tccaattacc tctatacaat caacagtaga aggtatttta gatggtatta
     1621 tatctgaaga agaagttaac tattatctca ataccatttc tcgtcaaaca aaccgtctta
     1681 accatctagt tgaagaatta agttttatta cattagagac tatgtcagac acggctgaac
     1741 ctcataaaga agaaacaatt tatctggata aactactgat tgatatttta tctgaatttc
     1801 aattggtgtt tgaaaaagaa aatcggcaag taatgattga tgttgcgcca gatgtttcga
     1861 aattatctag tcaatatgat aaattatcac gaattttatt gaatttgatt agcaatgcag
     1921 tcaaatattc tgatcctgga agtcccttga ctatcaaggc ctattcaaat cgtcaagata
     1981 tcgttatcga tattattgac caaggctatg ggataaaaga tgaagatctt gcttctattt
     2041 ttaatcgttt ttatcgtgta gaatcttcac gaaatatgaa gacaggagga catggcctag
     2101 gtctttatat tgcgcgacaa cttgcacatc aactgaatgg tgatattttg gtcgaaagtc
     2161 agtatcaaaa aggcagtaag ttctctttag tacttaaatt acaaaaatag ctaggaatta
     2221 ttcctagcta ttttttgtaa tgtttctata aaagattaag cgcgcagtag tttggtaagg
     2281 aaggagatag atatagacta atccgaaact gaagattgta aggaaatgcc aaccaataaa
     2341 acttaagtct aaaaggaaga gtctaagttt attaccattc attagttctc gactagcaac
     2401 tagtacttta gatggtccct catagtttcc actttctaat tgagtgtaaa atactaaagt
     2461 tgagatacta taggagtaat attgttgaac gagttttata actgccttag tagtaattag
     2521 gctactaaca actattaaaa caggggcaag gataaggtta ccactcaaaa gtagcctaaa
     2581 acttgaaatg atgattagta gaccaaaagt acttagtaga taaaagaaag ataaaattag
     2641 tccttttaga acaagtactg agagtaattt ccagaaaata tggcttgaaa atacataagt
     2701 attgtccgag aatctaacgt ttagtttctg atttttgacg acatctaaca ttacaaaact
     2761 gactgaaata ataaaaagtg tgtaaaagaa ataaactgga agaggtacaa aaaaatcaag
     2821 tagtgtcata ttattatat
//
LOCUS       DATRQA010000178         2817 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.178, whole genome shotgun sequence.
ACCESSION   DATRQA010000178 DATRQA010000000
VERSION     DATRQA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.178"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(73..753)
                     /locus_tag="VBD27_001904"
     CDS             complement(73..753)
                     /locus_tag="VBD27_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEO7025884.1"
                     /translation="MKEAKNFSQQLNILRAGVLGANDGIISVAGVVIGVASATHNLWI
                     IFLSAASAILAGAFSMAGGEYVSVSTQKDTEQAAVAREEKLLENNPELAKKSLVDIYL
                     AKGESHEHAQWLVDKAFSKNAIEHLVEEKYGIEFGEYTSPWHAAISSFIAFAIGSIFP
                     TITILLLPFSVRIVGTVIIVIVSLLSTGYVSAKLGQAPTVPAMRRNVMIGCLTMLATY
                     VIGQLFSI"
     gene            906..1934
                     /gene="queA"
                     /locus_tag="VBD27_001905"
     CDS             906..1934
                     /gene="queA"
                     /locus_tag="VBD27_001905"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO7025885.1"
                     /translation="MNTNDFDFYLPEELIAQTPLEKRDASKLLVIDHKNKTMTDSHFD
                     HILDELKPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTEGDQWEVLAKPAKRL
                     RVGNRVSFGDGRLIATVTKELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     RDRYQTVYAKENGSAAAPTAGLHFTKELLEKIETKGVKLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSKEAADTLNAVKESGGRIVAVGTTSIRTLETIGSKFNGELKADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGHDFVLEAYNHAVEERYRFFS
                     FGDAMFVK"
     gene            2123..2458
                     /locus_tag="VBD27_001906"
     CDS             2123..2458
                     /locus_tag="VBD27_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7025886.1"
                     /translation="MTYLQYVILLVLWEEDHRHMKELSYALSLNSNTLTPVLKRMENR
                     GWIVREKPRDDKRQLIIHLSNKGIESQDAVVNAVQSCGYFFSEISPESYQKTVSALSS
                     LNQVIKSHL"
     gene            complement(2513..>2817)
                     /locus_tag="VBD27_001907"
     CDS             complement(2513..>2817)
                     /locus_tag="VBD27_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7025887.1"
                     /translation="LWKHRKKYNSLITYLEEAFPTDYYRALIPSDVVVAQKPGYVREA
                     LNVGAIVKEEVPYIVAIYTAGLGGSTQEDSEINGVGLYQLEQLCFVINQWHRVNMN"
BASE COUNT          926 a          523 c          483 g          885 t
ORIGIN      
        1 cttaatttcg ttataaaact aaaaagaatt actgtccatt ctagtttaga caataactcc
       61 acgattaata ttttatattg aaaataattg tccaatgaca taggttgcca acattgtcaa
      121 acaaccaatc atcacatttc gacgcatggc aggtactgta ggggcttggc ctaacttagc
      181 acttacataa ccagttgata atagtgaaac aataacaatt attaccgttc ctacaattcg
      241 taccgaaaat ggtaataaca gaatagttat agttggaaaa attgatccga tggcaaaagc
      301 tataaaacta gatatagcag catgccaggg gcttgtatat tcaccgaact caataccata
      361 tttttcttca actaaatgct caatagcatt ctttgaaaaa gccttgtcaa ctagccattg
      421 agcatgttcg tgactttctc cttttgctaa gtagatatca actaaggatt ttttggcaag
      481 ttctggatta ttttctagta gtttctcttc acgcgcaact gctgcttgtt cagtatcttt
      541 ttgagtagaa acactaacat actccccgcc agccatagaa aatgcaccag ctaaaatcgc
      601 ggaagctgct gataaaaaaa taatccaaag gttatgagtt gcactcgcaa caccaataac
      661 aacaccagct acagagatga taccatcatt agcaccaaga acaccagctc ttaaaatatt
      721 caattgttgg gaaaaatttt ttgcttcttt catcttctct acttctcact ttctgacaat
      781 tattatacca cttatttaga atgattacaa ttatttttta taatcattct aaataagaag
      841 attagttggg tcatttttac cgaacgtgat ataatagaaa agcttactta tagataggag
      901 aaatcatgaa tacaaacgat tttgattttt atttaccaga agaacttatt gcccaaacac
      961 cacttgaaaa aagagacgct tctaaactat tagtcattga tcataagaat aagacgatga
     1021 cagatagcca tttcgatcat atcttagatg aactcaagcc tggtgacgct ttagtaatga
     1081 ataacacacg tgttctgccc gctcgccttt atggtgaaaa gccagataca cacggccatg
     1141 tagagttatt acttctcaaa aatacagagg gcgatcaatg ggaagtgctt gctaaacccg
     1201 ctaaacgctt acgcgtaggg aacagagttt catttggtga tggtcggtta atagcaacag
     1261 tcacaaagga attagaacat ggtgggcgta ttgtagaatt ctcatatgat ggtatttttc
     1321 tagaagtcct tgaaagtttg ggcgaaatgc cacttccgcc atatattcat gaaaaactag
     1381 aagatcgtga ccgctatcag actgtgtatg caaaagaaaa tggttctgct gctgcaccaa
     1441 cagccggtct tcattttaca aaagaactat tggaaaaaat tgaaacaaaa ggtgtgaagc
     1501 ttgtctattt gacgcttcac gttggacttg gaactttccg tcctgtttca gttgataatc
     1561 ttgatgaaca cgaaatgcat tctgagtttt accaattatc taaagaagct gctgatactc
     1621 tcaatgcagt taaagaatct ggtggacgta ttgtagctgt tggtacaact tctattcgca
     1681 cattagagac aattggttct aaattcaatg gtgaattaaa agccgactca gggtggacaa
     1741 atatttttat caagcctggt tatcagttca aggttgtaga cgctttttca accaactttc
     1801 acttaccaaa gtctactctt gtcatgttag tatcagcatt tgctggacat gactttgtac
     1861 tagaagctta caaccatgca gttgaagaac gatatcgttt cttcagtttt ggtgatgcta
     1921 tgtttgttaa gtaagaatag gctttcaatt aggaaaaagt tttatcgttt tttaacacga
     1981 ttgacatact ataacgcctc caaaatgcta caatatgata atgagtaatc gaagtatttt
     2041 agacgagcga ttatgtttta cggttagtga aacacatcga cttttcaata aattttacca
     2101 aaaagctttg gatgaatttc aattgactta tctccaatac gttatcttgt tagtcctttg
     2161 ggaagaggat catcggcata tgaaggaact tagttatgca ctatctctta attcgaatac
     2221 cctaactcca gttttaaagc gtatggaaaa tcgtgggtgg attgtacgag aaaaaccaag
     2281 agatgacaaa aggcaactca ttattcatct aagtaacaaa ggtattgagt cacaagatgc
     2341 cgttgtcaat gcggtgcaat cgtgcgggta ttttttctca gaaattagtc ctgaatctta
     2401 tcaaaaaact gtttctgccc tttcttcact taatcaagtt ataaaatcac atttataaaa
     2461 acctcttaga tagttaatct aagaggtttt tattattttg atagatttat ttctaattca
     2521 tattaacacg atgccattga ttaataacaa aacataattg ttctagctga tatagtccca
     2581 ctccattaat ttctgaatct tcctgagtag agccacctaa acctgcagta taaatagcaa
     2641 cgatataagg cacttcttct ttgacaattg ctccaacatt caacgcctca cggacatacc
     2701 ctggcttttg agcaacaaca acatcagatg gtatcaaagc acgataataa tccgttggaa
     2761 aagcttcttc taaataagta attaaactat tatacttttt acgatgtttc cagagat
//
LOCUS       DATRQA010000179         2804 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.179, whole genome shotgun sequence.
ACCESSION   DATRQA010000179 DATRQA010000000
VERSION     DATRQA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.179"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            6..86
                     /locus_tag="VBD27_001908"
     tRNA            6..86
                     /locus_tag="VBD27_001908"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:Tyr,seq:gta)
     gene            93..163
                     /locus_tag="VBD27_001909"
     tRNA            93..163
                     /locus_tag="VBD27_001909"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:125..127,aa:Trp,seq:cca)
     gene            178..250
                     /locus_tag="VBD27_001910"
     tRNA            178..250
                     /locus_tag="VBD27_001910"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:His,seq:gtg)
     gene            257..328
                     /locus_tag="VBD27_001911"
     tRNA            257..328
                     /locus_tag="VBD27_001911"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Gln,seq:ttg)
     gene            339..422
                     /locus_tag="VBD27_001912"
     tRNA            339..422
                     /locus_tag="VBD27_001912"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:373..375,aa:Leu,seq:caa)
     gene            798..1098
                     /locus_tag="VBD27_001913"
                     /pseudo
     CDS             798..1098
                     /locus_tag="VBD27_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1338..1541
                     /locus_tag="VBD27_001914"
     CDS             1338..1541
                     /locus_tag="VBD27_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025888.1"
                     /translation="MAESANTVRSNSFVLEELTEFQREKLKNGESVKFNFSKDVKKGN
                     QITQGGSNLPNDGSVYLQPNKSL"
     gene            1692..2132
                     /locus_tag="VBD27_001915"
     CDS             1692..2132
                     /locus_tag="VBD27_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein BUG3"
                     /protein_id="HEO7025889.1"
                     /translation="MKKHIKSIIPIVLIGMILGGCQMNSEHKSQYNETKSSKQSEVKK
                     DKKMTKKEQLAYLKEHEQEIIDFVKSQNKKIESVQIDWNDVRWSKGGNGTPQGGGEGI
                     LLFGEINNDSESSWRVDIDIEKGRLDLKNMYLGQPIRIGGKLFE"
     gene            2125..2469
                     /locus_tag="VBD27_001916"
     CDS             2125..2469
                     /locus_tag="VBD27_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025890.1"
                     /translation="MSNWNLESFDNLFATLAESTYTKRPSNFTYNELNPKLLRQLNSG
                     SSVELDFSKSVKKGKKVIEGGQNLPNDGIVYLQPDKSLKSIDEKVEVRVPDVNGGYHS
                     EHCVTNSYQKGY"
BASE COUNT         1003 a          380 c          545 g          876 t
ORIGIN      
        1 ccttaggaga gatagcgaag aggctaaacg cggcggactg taaatccgct ccttcgggtt
       61 cgggggttcg aatccctctc tctccatagt aaacgggcat agtttaaagg tagaactaag
      121 gtctccaaaa ccttcagtgt gggttcgatt cctactgccc gtgttttttc taatatggcg
      181 ggtgtggtga agtggttaac acatcagatt gtggctctga cattcgtggg ttcgattccc
      241 atcactcgcc ttttcatggg gtatagccaa gcggtaaggc aagggacttt gactccctca
      301 tgcgttggtt cgaatccagc taccccagtt ttaagatagc cggcgtggcg gaattggcag
      361 acgcgctgga ctcaaaatcc agtgtcctca cggacgtgcc ggttcgaccc cggccgccgg
      421 tatactttaa aagacaaggt ttctaaacct tgtctttttt tgttttgtac tggtaatttt
      481 aagaaaaagt tactgaaaat tacttatatt tgtttttcat tattgcaatt gttattgaag
      541 ttttaaaaca agcagaaata ataaggaaat ttttcaggag ttagtggaga ctcaacattt
      601 aaatgaaatt caaacaggac tcctaaatgt cataggttct gaagtaataa aagtctttgt
      661 gaatgataga taggtagtat aaaataataa gatttttcag tgtatatgtt acgactaaaa
      721 aattaaactc gtgaaaattt gttatactaa tattagtttt aaaatactgg taaataataa
      781 ttatttttat tagatacttg acatgggaag tttattttct aagaaaagag agcatgatga
      841 aaaaatatgt aaaatggtta attcccatat ctattattgg gatgttacta gcaggttgtc
      901 aaatgaatag tgaatctaaa agtcagtcaa acgaagtaaa aaatagcaaa cagtcagcag
      961 taaagaaagg taaaaaagta ataaaaaaag aacaagtagc ttatctcaaa aagtatgaaa
     1021 aagaattaac tgaatatgtc aaaacccaaa atccagatat tagcgaaatt aagtatgatt
     1081 ggaatagtgt gcgtatagtg gaagctagta atggaacccc tcaaggcgga gagcagctat
     1141 tattaatttg gggttatgca aatggcagtg aattaaccaa ttttactcta aatttcacat
     1201 tgggtgataa tcaaatgcct aatattgact cagttggctc agataattta tcaactattg
     1261 aagattaata ataacagtaa gaaaggtata taatgggatg ttggaatctt aaggaatttg
     1321 aagatatatt ttgaagtatg gcagaaagtg ctaatacagt tcgatcgaat tcttttgttt
     1381 tagaagaatt aactgaattt caaagagaaa agttaaaaaa tggggagtca gttaagttta
     1441 atttttctaa agatgttaag aaaggaaatc aaatcaccca aggtggctca aatctcccaa
     1501 atgatgggag tgtctatctt cagcctaata agtccttata aactgtttat taaactgaaa
     1561 aaattgaagt tttcatagat aaaattcttc gagtaaaata ttagagatgc tatttatatt
     1621 tcttaagcta gtaatttttt attgtttatt ggtttaatga taatacaaat ataaaatagg
     1681 tggtgcaaat aatgaaaaaa catataaaaa gtatcatacc aatagttctt attggtatga
     1741 tactaggagg ctgtcaaatg aatagtgaac ataaaagtca gtataatgaa acaaaaagta
     1801 gcaagcaatc agaagtgaag aaagataaaa aaatgacaaa aaaagaacaa ttagcttatc
     1861 tcaaagagca tgaacaagaa ataattgatt ttgtaaaatc tcagaataaa aagatagaat
     1921 ctgtacaaat tgattggaat gatgttcgat ggagtaaagg gggaaatggt acacctcaag
     1981 gaggaggaga ggggatttta ctttttgggg agattaataa tgattctgaa tcaagttgga
     2041 gagttgatat tgatatagaa aaaggacggc tagacctaaa aaatatgtat ttaggacaac
     2101 ctatacgaat tggaggtaaa ttatttgagt aattggaact tagaaagttt tgataaccta
     2161 tttgctactt tagcagaatc aacatacaca aaaagaccaa gtaactttac atataatgaa
     2221 ttgaatccta agttgctacg acaactgaac tcaggtagtt ctgttgaatt ggatttctca
     2281 aaatctgtta agaaaggaaa aaaagttatc gaaggcggtc agaacctccc caatgatggt
     2341 attgtctacc tgcaaccaga taagtcctta aaatctattg atgaaaaagt agaggttcgc
     2401 gttcctgatg tcaatggcgg ttatcactct gagcattgtg ttactaattc ctatcagaaa
     2461 ggttactgaa agatgatgaa gcagtggctc tataatcact aaaagctcat cattgctcag
     2521 tgtttaagtt aacttctaaa gctcgaacct tgttaaatca aggtttgttt acttttaaaa
     2581 gtgacatttt atttttctaa aaataaggtt atcgggattc ctcattctaa gttactttgt
     2641 ttatatgtac ctttacttaa ggtttatttt cattattttt aatttaatat tttggagtat
     2701 gtacgctcca ttatagtatt ttcgaagaca tctttaaaag aactgtagaa gttaaaatat
     2761 tttaacctag tatctaacgt catatattgg tacatagttt taaa
//
LOCUS       DATRQA010000180         2759 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.180, whole genome shotgun sequence.
ACCESSION   DATRQA010000180 DATRQA010000000
VERSION     DATRQA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.180"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(65..826)
                     /locus_tag="VBD27_001917"
     CDS             complement(65..826)
                     /locus_tag="VBD27_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7025891.1"
                     /translation="MIQINNLHKFYGQKEILKDINISIPKGKVTAILGPNGSGKSTLL
                     SCISRLEPYDNGEIFLDKVPLAHYSSNDLAKTLAILRQSNHLTLKIKVSDLIGFGRFP
                     YSKGRLSQKDKAVIESVISYMDLNDIADEFINNLSGGQIQRAFIAMTMAQDTQYICLD
                     EPLNNLDMKYAVQMMDLIKRYAYEFNKTIVIIIHDINFATHYADNVVALKEGQVVSCG
                     TVEDVMQEKILSHLFDMPIRIETVDGKPIILFCTT"
     gene            complement(823..1797)
                     /locus_tag="VBD27_001918"
     CDS             complement(823..1797)
                     /locus_tag="VBD27_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO7025892.1"
                     /translation="MIKDLEIPKLLILLILLIATIILFLIYGIPTDANEFLIIYILKT
                     RYQKLIALILVGICIGSSSLIFQTLTNNRLLTPSIIGLDSLYVLIQTGLMYLIGAQRV
                     IKFSSFSSFLLSLLLMVGFAYLLFTILFRNKKQSLYFVLLAGLIFNTLFSSISSFIQA
                     IMDPNDFMILQNQLFASFNAINTKILWISFIIIVVSFVINWPFIKELDVLLLGKENAI
                     SLGISYQKLTTRIFLWLALMVAIATALVGPITFLGLLVAHITYHSFHTFRHQILVPIA
                     IVICIFTLVLGQHLVQNLLHLTVQLSVLLNLIGGSYFIFTLIKGRKNL"
     gene            complement(1794..2756)
                     /locus_tag="VBD27_001919"
     CDS             complement(1794..2756)
                     /locus_tag="VBD27_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO7025893.1"
                     /translation="MKKLLTTPVMGCTLVILSLTSLFVGVKSIPLEQITHLNQSQVDI
                     FLASRLPRTISILISGASLSVCGLLMQQLTQNKFVSPTTSGTMDWAKLGVVVTLIFFK
                     NTSIFIQLCIASGFAILGSLLFVTILKMITFKDNIFIPLIGLMLGQIVAAATVFLGTH
                     FQVLQSVNSWLQGNFSIMTSHRYEILYLALPCLFLVYFFAHQFTIVGLGESFAKNLGI
                     AYEKMIYFGLVLVSIMTSLVIIIVGALPFLGLIVPNLISLTKGDHMSSTILETSLLGA
                     CIVMICDLFGRLVIFPYEVSIGVTLGVLGSAFFLISIIRNEGKS"
BASE COUNT         1018 a          449 c          459 g          833 t
ORIGIN      
        1 cattaaataa aatctcctaa aatagataaa aactaaaaat gttatcctac ataaatctct
       61 ttatttacgt tgtacaaaaa aggataattg gtttaccatc aacagtttcg atacggatag
      121 gcatatcaaa taaatgagaa aggatttttt cttgcataac atcttcgaca gtcccacaag
      181 acaccacttg tccttctttt aaagcgacaa cattgtcagc gtagtgagtt gcaaaattga
      241 tgtcgtgaat aataatcact atagttttat taaactcata cgcatatcgt ttgataagat
      301 ccatcatttg aacagcatat ttcatatcta aattgttgag tggctcatct aagcaaatat
      361 attgagtatc ttgtgccatt gtcattgcga taaaagctct ttgaatttga cctccgctga
      421 ggttattaat gaactcgtca gctatatcgt ttaaatccat ataagatata actgattcta
      481 tgacagcttt atctttttga cttaaccgtc ctttactata aggaaatcta ccgaatccta
      541 tcaagtcact cacctttatc ttgagtgtta agtgattaga ttgtcttaat atggctaatg
      601 ttttggctaa gtcatttgaa ctgtagtggg ctaatggtac tttatcaagg aaaatttcac
      661 cattatcata aggttcaagt cgactgatac aactcagcaa tgtactcttt ccagatccat
      721 ttggacctaa aatagctgtt acttttcctt taggaatcga aatattaatg tcttttaata
      781 tttccttttg gccatagaac ttatgtaaat tgtttatttg aatcataagt ttttccttcc
      841 tttgattaag gtgaatataa aataacttcc cccgataagg ttgaggagaa cacttagttg
      901 aacagtgagg tgtaatagat tttgaacgag atgctgccct aagacaagtg taaagataca
      961 gataacaatg gctataggaa ctaatatttg atgtctaaag gtatgaaaac tgtgataggt
     1021 aatatgtgct acaagtaagc ctaaaaaagt aatcggccca actaatgctg ttgctatagc
     1081 taccatcaat gctaaccata aaaaaattct agtagtaagc ttttgatatg agatgccgag
     1141 tgatatcgca ttttctttac ccaatagtaa gacatccaat tcctttataa aaggccaatt
     1201 aatgacaaag ctaacgacaa tgataatgaa agaaatccaa aggatcttgg tattaatagc
     1261 attgaaacta gcaaagagct gattttgtaa aatcataaaa tcgttggggt ccataatggc
     1321 ttgaataaag gatgagatag aagaaaataa tgtattaaat atgagtccag ctaatagaac
     1381 aaaatacaaa gactgttttt tatttcgaaa aagtatcgta aataataagt aagcgaatcc
     1441 aaccattaat aataatgaaa ggaggaagct gctaaaagat gaaaatttta tgacacgctg
     1501 agctccgatt aaatacatta atcctgtttg gataagaaca tataagctat ctaaaccaat
     1561 aatggacgga gttaaaagtc ggttattggt tagagtttga aaaataagac tagaactacc
     1621 aatgcagata ccaaccaaaa ttaatgcaat caatttttga taacgtgttt ttaggatata
     1681 gataatcaaa aattcatttg cgtcagttgg aataccataa attaaaaaca aaataatggt
     1741 agcaattagt agtatcagta aaataagtaa ttttggtatc tctaaatcct taatcatgat
     1801 tttccctcat ttcttataat tgaaatcaag aaaaaggcac tacccaatac tcctagcgta
     1861 actcctattg agacttcata aggaaaaata actaagcgac caaaaagatc acatatcatg
     1921 acgatacaag cccctaaaag cgatgtctct aagatagttg aggacatatg atccccttta
     1981 gttaagctaa ttagattagg tacgattaaa cctaagaaag gtaaagctcc tacgataata
     2041 atgactaggg atgtcataat tgaaaccaaa actaaaccaa agtaaatcat cttttcataa
     2101 gcaatcccta aatttttagc gaagctttct ccaagcccaa caatggtaaa ctgatgagca
     2161 aaaaaataca ctaaaaacaa acatggcaat gctaagtaaa gaatttcgta gcgatggcta
     2221 gtcatgatgg aaaaattacc ttgaagccaa ctgttgacag attgtagtac ttgaaaatgt
     2281 gttcctaaaa atactgttgc tgctgctaca atttgtccaa gcatcagtcc gataagaggt
     2341 ataaaaatat tatccttaaa agtaatcatt tttaaaatag ttacaaaaag cagcgatcct
     2401 aaaatggcaa aacctgaagc aatacatagt tgaataaaaa tagaggtatt tttaaagaaa
     2461 atcagcgtaa caactactcc taatttcgcc caatccatag tccctgaagt tgtcggtgaa
     2521 acgaacttgt tttgggtgag ttgttgcatt agcaggccac agactgacaa acttgcacca
     2581 gatattagga tactaatggt tcttggtaag cgactcgcta aaaaaatatc tacctgtgat
     2641 tgattaaggt gtgttatttg ctccagtggg atagatttaa caccaacaaa tagggacgtt
     2701 agagaaagta taaccaaagt acaacccatt acaggagtgg ttaatagctt tttcataaa
//
LOCUS       DATRQA010000181         2746 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.181, whole genome shotgun sequence.
ACCESSION   DATRQA010000181 DATRQA010000000
VERSION     DATRQA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.181"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            6..464
                     /locus_tag="VBD27_001920"
     CDS             6..464
                     /locus_tag="VBD27_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025894.1"
                     /translation="MRKYIKWLIPISIFGMILGGCQMNSEPKSQSNEVKNSKQSEVKK
                     DKKMTKKEQLAYLKEHEQEIIDYVKLHNNQIESVQFDWSSVKVEQSGNGTPQGGDYNL
                     SLRGKFNHLQNSKLIVDFYLAHKNDIPNIKSMGMLNKPYIHKNGIWHIYE"
     gene            528..2162
                     /locus_tag="VBD27_001921"
     CDS             528..2162
                     /locus_tag="VBD27_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7025895.1"
                     /translation="MSNWNLDNFDNLHSSLAESAYNSRPNSFPELFETDSVTEVKFSQ
                     PSEDNKGQITQGGTNLPNDGIVYLQPDKSLKSIDENVKVLIPDVNGGYHTEHYVTHSY
                     QKGVLTDDKAGFNAYYLSDTEKIDSTTKHTYLAIRGSDGIGLDTLNDWVSNNAMFAVS
                     NKYIPQAKLANKAMKEKIAELKGKAPGAVMDITGHSLGTIVSSQAVVNLSYAELENVG
                     QVVLFDGPDVSRSLEKMEGISAKKIQEAGKHVTYYVNPFDIVSMLNREKPWKDQFGKV
                     NVIVPSKYTGTFDEQSSHDFGAFQIDSSGNPLVASESYHPELLTSGQDLARLEKKFIK
                     NLLGEHYDSDKLGPVLKGALTGDLTRLIATLKACGISVTVNEAKKLYDNFQKDYKAII
                     SKAKKDGLEWDRKNIASFHSRIKSASGSQRILLRTQMLYMATQLAEADVSEKVSATKK
                     HISEAKDTIVEISTSTISSAEIMAMHLGQSEVDALVSDIKMSTVWNDSVETSDYEALD
                     HYKTKMTTFTTNLVTVAQNLTAQDEQLAGDIVTNLS"
     gene            2181..2453
                     /locus_tag="VBD27_001922"
     CDS             2181..2453
                     /locus_tag="VBD27_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025896.1"
                     /translation="MSKELKLPLNKMLKEQERIAIKAIVATAKSTITDTGTNLKATIE
                     TDAQDLKNYASTYNANITDGLEGQSAQAASDFLTQLPKPELDNPVN"
     gene            2463..>2746
                     /locus_tag="VBD27_001923"
     CDS             2463..>2746
                     /locus_tag="VBD27_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025897.1"
                     /translation="MVDTKKLQELDQEYDQNLRNIYRNREQLEDDFHLFMARTDSLKE
                     SVYQATLGQGWELPQEAHAHLYNMDDNKDTFISEFNEYMEKLEEKEIELR"
BASE COUNT         1041 a          469 c          515 g          721 t
ORIGIN      
        1 acatgatgag aaaatatata aaatggttaa ttcccatatc aatttttggg atgatacttg
       61 gaggctgtca aatgaatagt gaacctaaaa gtcagtcaaa cgaagtaaaa aatagcaagc
      121 aatcagaagt gaagaaagat aaaaaaatga caaaaaaaga acaattagcc tatctcaaag
      181 agcatgagca agaaatcata gattatgtaa aattacataa caaccaaatt gagtccgttc
      241 aattcgattg gtcaagtgta aaagtagaac aaagcgggaa tggaactcca caagggggtg
      301 attataatct ttcactgaga ggaaagttta atcatctaca aaattcaaaa ttaatagttg
      361 atttttattt agctcataaa aatgatatcc caaatatcaa atcaatggga atgctaaata
      421 agccatatat acataaaaat ggtatttggc acatttatga atagcttgaa tcatcctata
      481 taaagtaata gaatttttac cacaagcaag gtagaggaga agaataattg agtaattgga
      541 acttagataa ttttgataac ttacatagct ctttagcaga aagtgcatat aatagtcgac
      601 ctaattcatt tcctgaatta tttgagactg actcagtaac agaagtaaaa ttttctcaac
      661 cctcagaaga taacaaaggt caaatcacac agggtggcac taacctcccc aatgatggta
      721 ttgtctacct gcaaccagat aagtccttaa aatctattga tgaaaatgta aaggtcctca
      781 ttccagatgt caatggaggc tatcacactg agcattatgt tactcattct tatcaaaaag
      841 gtgtgcttac tgacgataag gcaggtttta atgcttacta cctgtcagat actgaaaaaa
      901 tagatagcac tacaaagcac acttatctag ccattcgtgg tagtgatggg attggtctag
      961 atacactaaa cgattgggtt agtaacaatg caatgtttgc tgtctctaac aagtatattc
     1021 cacaagcgaa attggctaat aaggcaatga aagaaaaaat tgccgagcta aaaggaaaag
     1081 cacctggagc tgttatggat attactggtc actcactcgg tacaattgta tcatctcaag
     1141 cagtagttaa tcttagctat gcagaattgg agaatgttgg tcaagtagtc cttttcgatg
     1201 gccctgatgt atcaagaagc ttggaaaaaa tggaaggcat ttctgctaaa aaaatccaag
     1261 aagctgggaa gcatgttacc tactatgtca atccctttga tatagttagt atgcttaatc
     1321 gtgaaaagcc atggaaagac caattcggga aagtaaatgt cattgttcct agtaaatata
     1381 cgggtacttt tgatgagcaa tcttctcacg attttggtgc cttccaaatt gatagtagcg
     1441 gaaatccttt agttgcttct gaaagctatc atccagaatt attaacatct ggtcaagact
     1501 tagctagact cgaaaaaaaa ttcataaaga atcttttagg agagcattat gattcggata
     1561 agctaggacc agtactcaaa ggcgctctga ctggggacct gactcgacta attgcaacat
     1621 taaaagcttg tggtatcagt gtcactgtaa acgaagctaa aaaactgtac gataacttcc
     1681 aaaaagacta caaagccata atttccaaag ctaaaaaaga tggcctcgag tgggatcgca
     1741 aaaacatcgc aagcttccat agtaggatca aatcagcatc aggcagtcaa cggattttac
     1801 ttagaactca aatgctctat atggcaacgc aattagcaga agcagatgtt tcagaaaaag
     1861 tctctgcaac aaaaaaacat attagtgaag ctaaggatac gattgtagaa atatcgacat
     1921 ccactatttc atcagctgaa ataatggcta tgcaccttgg ccaatcagaa gttgatgctc
     1981 tggtttcaga tataaaaatg tcaacagttt ggaatgatag cgtagagact tctgattatg
     2041 aagcacttga tcactataaa acaaaaatga ccacatttac gaccaattta gtaacagttg
     2101 cacaaaattt aacagctcaa gatgagcaac tagctggtga tattgtcaca aacttatcct
     2161 aaaatagaga gattaaatga atgtccaaag aattgaaatt gcccttgaat aaaatgttga
     2221 aagaacaaga aagaatagct attaaggcaa tagttgctac agcaaaaagc actattacgg
     2281 atactggaac aaatttaaaa gcaaccatag agacagatgc ccaagaccta aaaaactatg
     2341 catccaccta taacgccaat atcacggatg gtcttgaagg ccagtcagct caagcagcat
     2401 ctgatttctt gacacaactt ccaaaaccag agttagataa tccagtcaac taggaggcta
     2461 gtatggtaga tacaaaaaaa ctgcaagaac tcgatcaaga gtatgatcaa aatctcagaa
     2521 acatttatcg taacagagag caactagagg atgactttca cttgtttatg gcaagaacag
     2581 atagcctaaa agagtctgtc tatcaagcaa ctttaggtca aggttgggaa ttaccccaag
     2641 aagctcatgc acacctatat aatatggatg ataataagga tacattcata tcagaattca
     2701 atgaatatat ggaaaagctt gaagaaaagg aaatagagct tcgaag
//
LOCUS       DATRQA010000182         2732 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.182, whole genome shotgun sequence.
ACCESSION   DATRQA010000182 DATRQA010000000
VERSION     DATRQA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.182"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..635
                     /locus_tag="VBD27_001924"
     CDS             <1..635
                     /locus_tag="VBD27_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO7025898.1"
                     /translation="SSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTE
                     LYMEKADFQVQFTKGKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKS
                     AFSRKEDKTSIVFDEVDTGVSGRVAQAIAQKIHKIGSHGQVLAISHLAQVIAIADYQY
                     FIEKISSDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS"
     gene            748..1584
                     /locus_tag="VBD27_001925"
     CDS             748..1584
                     /locus_tag="VBD27_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7025899.1"
                     /translation="MSKIKIVTDSSITIEPELIKELDITVVPLSVMIDGTLYSDNDLK
                     AQGEFLNLMRGSKELPKTSQPPVGVFAEIYEKLMNEGAEHIIAIHLTHTLSGTIEASR
                     QGANIAGADVTVIDSTFTDQCQKFQVVEAAKLAKEGADLDTILARVEEVRQKSELFIG
                     VSTLENLVKGGRIGRVTGLLSSLLNIKVIMELTNHELVPIVKGRGLKTFSKWLDNFVE
                     SAQTRKIAEIGISYCGKADMANNFKEKLAVLGAPISVLETGSIIQTHTGEDAFAVMVR
                     YE"
     gene            1577..2416
                     /locus_tag="VBD27_001926"
     CDS             1577..2416
                     /locus_tag="VBD27_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO7025900.1"
                     /translation="MNKKKILTGLSFFLVSLLLSFGIFSLIIPKSNPKLTKKDFLTKK
                     VIPLNYVALGDSLTEGVGDTTSQGGFVPLLSESLHNRYSYQVTSVNYGVSGNTSQQIL
                     KRMTTDPQIEKDLEKADLLTLTVGGNDVLAVIRKELSHLSLNSFEKPAEAYKERLKEI
                     LAKARQDNPKLPIYVLGIYNPFYLNFPQLTKMQTVIDNWNKATKEVVDASENVYFVPI
                     NDRLYKGINGKEGITESSNSQASITNDALFTGDHFHPNNIGYQIMSNAVMEKINETRK
                     NWP"
     gene            2391..>2732
                     /locus_tag="VBD27_001927"
     CDS             2391..>2732
                     /locus_tag="VBD27_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEO7025901.1"
                     /translation="MKQEKTGRNLNFWKWAFLLLLAINLSFTAVIASRLIQVREPNTG
                     KISTGVQDKVKVGTFTTNKSQLNKTIALYLKQYQTKKMNYKIYAASSSILFEGSYQLL
                     GYEVPLYIYFEP"
BASE COUNT          940 a          430 c          517 g          845 t
ORIGIN      
        1 agtcctcaga tgctttagaa aaagaattaa agattttaga acacgatttg attgaatcag
       61 caaatcaatt aagtttagag cgtcataagc tagctaaaca attggaaaat gaaatcaaac
      121 aagaattaac agagctttat atggagaaag ctgatttcca agttcaattt acaaaaggaa
      181 aatttaataa agaagggaac gaaatcgttg aattttatat ttcaacgaat cccggagagg
      241 gctttaagcc attggtaaaa gtggcttcag gaggagagtt atctcgattg atgttagcta
      301 ttaaatctgc tttttcacgt aaggaagata aaacttccat tgtatttgat gaggtggata
      361 caggagtttc tggtcgcgtg gcccaggcta ttgctcaaaa aattcacaag attggtagtc
      421 atggacaagt tttagcaatt tctcatttag ctcaggttat tgcaatagcg gattaccaat
      481 attttattga aaaaatttcc agtgactctt caacagtttc aacggtaagg cttttaagct
      541 atgaagaacg tgtagaagaa atcgctaaaa tgttggcagg aaacaatgta actgatactg
      601 cacgtactca agctaaagaa ttacttggta gttagctttg gtaacgtctt taaaaagcta
      661 acattgttag gagaaattaa tgttgataca cttaaatttt gttataataa gggtattgaa
      721 atattaaagt atagaatagg ataggatatg agtaaaatca aaattgtaac ggattcttcc
      781 attaccattg aaccagaact tattaaagag cttgacatta cagttgttcc cttgtcagtt
      841 atgatagatg gtaccttgta ttcagacaat gacttaaaag cacaagggga attcttgaac
      901 ctaatgcgtg gaagtaagga attgccaaaa acaagtcaac cacctgtagg tgtttttgca
      961 gaaatttatg aaaaattaat gaatgaaggt gcggaacata tcattgcaat tcatttaacg
     1021 catacactat caggaactat tgaagcatca cgccagggag ctaatattgc tggtgcagat
     1081 gttacagtta ttgattctac ttttacagac cagtgtcaaa aattccaggt tgtagaagcc
     1141 gcgaaattag ctaaagaggg agctgattta gatacaattt tggctcgtgt ggaagaagta
     1201 cgccagaagt cagaattatt tattggcgta tcgacccttg aaaatttagt aaaaggtggt
     1261 cgtattggac gtgtaacagg tctcctaagt tcattgctta acataaaagt aattatggag
     1321 ctaacaaacc atgaattagt accgattgtt aaaggtcgcg gtctaaagac cttcagcaaa
     1381 tggttagata attttgttga aagtgcccaa acacgaaaaa ttgctgaaat tggcatctcc
     1441 tactgtggta aagctgatat ggctaataac tttaaagaaa aactagctgt tctaggtgct
     1501 cctatatccg tattagaaac tggctcaatc atccaaactc atactggtga ggatgcattt
     1561 gctgttatgg ttcgttatga ataagaaaaa aatacttacc ggactttctt tttttctggt
     1621 aagtcttctt ttatcttttg ggattttttc cttgatcatt ccaaaatcaa atcctaaatt
     1681 aacaaaaaaa gacttcctaa caaagaaagt tatcccactt aactatgttg ctcttggaga
     1741 ttctctgacc gaaggtgtgg gggatacaac ctctcaaggt ggttttgtcc cactgctatc
     1801 agaatcactc cataatcgat actcttacca agtgacttct gttaattatg gtgtgtctgg
     1861 gaatactagt caacaaattt taaaacgtat gacgacagat cctcaaatcg aaaaagattt
     1921 agagaaagct gatttattga cgctaactgt tggtggtaat gatgtcttgg ctgttattcg
     1981 taaagagctc agtcatttat cactaaattc ctttgagaaa ccagcagaag catataagga
     2041 acgtttgaaa gaaattcttg caaaagcaag acaagataat cctaaattgc ctatttatgt
     2101 tttaggcatt tataatcctt tttacctaaa ctttccacaa ttaactaaaa tgcaaaccgt
     2161 tattgataat tggaataaag ctacaaaaga agtagttgat gcttcagaaa atgtttattt
     2221 tgtcccaatt aatgaccgcc tttataaggg aataaatggt aaagagggta ttacagagtc
     2281 atcaaatagt caggcaagta tcactaatga tgctctcttt actggagacc attttcatcc
     2341 caataatatt ggctatcaaa tcatgtctaa cgccgttatg gagaaaataa atgaaacaag
     2401 aaaaaactgg ccgtaattta aatttttgga aatgggcatt tctcttattg ttggcaatta
     2461 acctttcctt cacagcagtg attgcaagtc gtttaattca agtacgtgag cctaatacag
     2521 gaaaaatttc gactggggta caagataaag taaaagtagg tacttttacg accaataagt
     2581 cgcaactgaa taagacaatt gcactttatt taaaacaata tcaaactaag aagatgaatt
     2641 ataagattta tgctgcttca tcttctatac tatttgaagg ctcttatcaa ttattaggtt
     2701 atgaagtgcc tttatacatc tattttgaac ct
//
LOCUS       DATRQA010000183         2724 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.183, whole genome shotgun sequence.
ACCESSION   DATRQA010000183 DATRQA010000000
VERSION     DATRQA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.183"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(77..1459)
                     /locus_tag="VBD27_001928"
     CDS             complement(77..1459)
                     /locus_tag="VBD27_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7025902.1"
                     /translation="MLTTKENFKGNNKLLVGIVLAVLSFWLFAQSILNMGPDVQSSLG
                     ISSGAMDIGVSSTALFSGLFIVVTGGLADKLGRVKFTFIGLCLNIIGSLLIVLANGAV
                     LFIMGRIFQGLAAAFIMPSTMALVKTYYDGKDRQRAVSFWSIGSWGGSGLCSYFGGAV
                     ASTLGWRYVFIFSIIASVVSFLLILGTPESKNVGQKTHFDYLGLIIFIISMLSLNIGI
                     SMAQEHGLMNVIPLSLFTVMLIGFVLFYYVETRKSNSFIDFHLFENRFYLGATISNFL
                     LNAVAGTLIVINTYMQQGRQLTPKVAGEMSLGYLVCVLIAIRVGEKILQRFGARKPML
                     LGAMSTFVGIFLMTLVNIQGPLYLVLVFVGYALFGTGLGIYATPSTDTAISSIPNEKV
                     GSASGIYKMASSLGGAIGVATSIAIYHAFSGNADFHKAALCGLILNLVFCSLSILSIL
                     FVIPKKIEDK"
     gene            complement(1467..2681)
                     /locus_tag="VBD27_001929"
     CDS             complement(1467..2681)
                     /locus_tag="VBD27_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO7025903.1"
                     /translation="MANASLRHQLFEKLDQKCDQMVAIRRYLHENPELSFKETKTAAY
                     ISDFYKGKDCHVQTQFGGMNGVVVDIYGDKATDKPIKHIALRADFDALPIQEETGLSF
                     ASKTAGVMHACGHDAHTAYLLILAESLIELKSEFSGHIRILHQPAEEVPPGGAKAMIE
                     AGCLDGIDAVLGIHVMSTMEEGTVQYHAGPIQTGRATFKVILQGKGGHGSMPHRANDT
                     IVAASSFVMAAQTIVSRRVNPFDTAVVTIGSFDGKGSANVIKDSVTLEGDVRVMSEET
                     RGVVEEEFKRILDGIAQTYGVSYQLDYQNDYPVLVNNSEVTQKVANSLKSVAIKEILD
                     VIDCDPQTPSEDFAYYAQTIPACFFYVGAHEEGQPYYPHHHPKFQIAESSLMVSAKSM
                     ATAALAMLVEGE"
BASE COUNT          928 a          575 c          421 g          800 t
ORIGIN      
        1 tattttaaat ttctttccca attcctttta cattattaca acaatacttg agattttgaa
       61 tctcaagtat tgttatttat ttatcctcaa ttttcttagg gataacaaaa agaattgata
      121 agatcgatag actacaaaaa actaggttta aaattaatcc acacaaagct gctttgtgaa
      181 aatctgcatt accagaaaaa gcatgataaa tcgcaattga agttgctaca ccaattgcac
      241 cacctagtga acttgccatt ttgtaaattc cagatgcaga acctactttt tcattaggaa
      301 tactagaaat agctgtatct gttgatggtg tcgcatagat ccccaagcct gttccaaata
      361 aagcataacc aacaaagact aacacaagat acaaaggtcc ttgaatattg accaaagtca
      421 ttaaaaatat cccaacaaat gtactcatag ctcccaaaag cattggctta cgggcaccaa
      481 aacgttgtaa aattttctca ccaactcgga tagcaattag tacacaaacc aaatagccta
      541 aagacatttc acctgcaacc tttggtgtta gctggcgacc ttgttgcatg taagtattaa
      601 taacaatcag agttcctgca actgcattca gtaagaaatt tgatattgtc gcacctaagt
      661 aaaatcgatt ttcaaacaga tgaaagtcaa taaaactatt agatttacgt gtttcaacat
      721 aataaaacag aacaaatcca ataagcatca ctgtaaaaag actcaatgga ataacgttca
      781 tcaatccatg ttcctgagcc atagatatcc caatatttaa cgataacatt gaaataataa
      841 aaataattaa accaaggtaa tcaaagtgag ttttttgtcc aacattttta ctttctgggg
      901 ttcctaaaat taatagaaaa ctaactactg atgcgatgat tgaaaagata aagacataac
      961 gccaacctag agtacttgcc accgctccac caaaataaga acatagacct gagccacccc
     1021 acgatccgat tgaccagaaa ctaactgcac gttgtctatc tttaccatca tagtacgttt
     1081 taaccaaggc cattgttgat ggcataataa aagctgctgc caatccttga aaaatacgtc
     1141 ccataataaa tagcactgct ccattagcta aaacaattag tagagaacca ataatattta
     1201 agcaaagacc aataaaagta aatttaactc gaccaagttt atctgctaaa ccaccagtta
     1261 caacaataaa taaacctgaa aacaatgccg tacttgagac accaatatcc attgcaccag
     1321 atgaaatacc taatgatgac tgaacatctg gtcccatgtt taaaatagac tgtgcaaata
     1381 accaaaatga tagtacagcg agtacaattc ccaccaataa tttgttattt cctttgaaat
     1441 tttcctttgt cgttaacata tgttatctat tctccttcta caagcatagc aagcgctgct
     1501 gttgccattg atttagcaga taccatcaat gatgattctg caatttggaa ttttggatga
     1561 tgatgtgggt aatatggttg cccctcttca tgtgctccaa catagaagaa gcaagctggg
     1621 attgtttgag cataataggc aaaatcttca gacggcgttt gagggtcgca atcaatgaca
     1681 tctaaaattt cttttatagc aacagatttt aaactatttg caactttctg tgtcacttca
     1741 ctgttattaa ctaaaacagg ataatcattc tgatagtcta actgataact aacaccataa
     1801 gtttgtgcta ttccatctag aatacgctta aattcctcct caacaacacc tcgtgtctct
     1861 tcagacatca cgcgcacatc accttctaag gttacactat ctttgatcac attagcagaa
     1921 cctttaccat caaaagatcc gatagtaact actgctgtgt caaatggatt aactcgacga
     1981 ctgacaatcg tttgcgctgc cataacaaaa ctagaagctg caacaattgt atcatttgca
     2041 cgatgtggca tggaaccatg tccaccctta ccttgcaaaa taactttaaa agtggcacgc
     2101 cctgtttgaa tgggacctgc gtgatattgt accgttccct cttccatcgt tgacataaca
     2161 tggataccaa ggacagcatc aatgccatct aaacatcctg cttcaatcat tgcttttgct
     2221 ccgcctggtg gcacctcttc agccggttga tgtaagatac ggatatgacc tgaaaattct
     2281 gattttaatt caatcaagct ctcagccaaa atcaataaat aagctgtatg ggcatcatgt
     2341 ccacaagcat gcatgacgcc cgctgttttt gaagcaaaag aaagccctgt ctcttcctga
     2401 attggaagag catcaaagtc ggctctcaaa gcaatgtgtt tgatgggctt atctgtcgct
     2461 ttatcaccat agatatcaac tacgacgcca ttcataccac caaattgtgt ctgaacatga
     2521 cagtccttac ctttataaaa atctgaaata taagctgctg tttttgtctc tttaaatgat
     2581 aattcgggat tttcatgtaa atagcgacga atagcaacca tttgatcaca tttttggtca
     2641 agtttttcaa ataattgatg tcgcaaagat gcattggcca tttgctttcc tccttaaaat
     2701 atgaacagtt aaaataaggc tatt
//
LOCUS       DATRQA010000184         2694 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.184, whole genome shotgun sequence.
ACCESSION   DATRQA010000184 DATRQA010000000
VERSION     DATRQA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.184"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            69..737
                     /locus_tag="VBD27_001930"
     CDS             69..737
                     /locus_tag="VBD27_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7025904.1"
                     /translation="MSQEQGKIYIVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEI
                     IAFQPDLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFI
                     SKPFSLAVLDAKLTAILRRSQQFIQQELTFGGFTLTREGLLSSQDKEVILSPTENKIL
                     SILLMHPKQVVSKESLLEKLWENDSFIDQNTLNVNMTRLRKKIVPIGFDYIHTVRGVG
                     YLLQ"
     gene            734..1672
                     /locus_tag="VBD27_001931"
     CDS             734..1672
                     /locus_tag="VBD27_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7025905.1"
                     /translation="MIRQFLREHLIWYILYIMMFVLFFISFYLYHLPMPYLFNSLGLN
                     VIVLLGISIWQYSRYRKKMLHLKYFNSSQDPSFELQPSDYAYFNIITQLEAREAQKVS
                     ETIEQTNHVALMIKMWSHQMKVPLAAISLMAQTNHLDPKEVEQQLLKLQHYLETLLAF
                     LKFRQYRDDFRFEAVSLREVVVEIIKSYKVICLSKSLSIIIEGDNIWKTDKKWLTFAL
                     SQVLDNAIKYSNPESKIIISIGEESIRIQDYGIGILEEDIPRLFEDGFTGYNGHEHQK
                     ATGMGLYMTKEVLSSLNLSISVDSKINYGTAVSIHK"
     gene            complement(1715..>2694)
                     /gene="xerS"
                     /locus_tag="VBD27_001932"
     CDS             complement(1715..>2694)
                     /gene="xerS"
                     /locus_tag="VBD27_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEO7025906.1"
                     /translation="PYSFTTLYEYLKEYRRFLEWLLDSGVANCHHIAEIELSVLENLT
                     KKDMEAFILYLRERPLLNANTRQNGVSQTTINRTLSALSSLFKYLTEEVENADGEPYF
                     YRNVMKKVSTKKKKETLASRAENIKQKLFLGNETIEFLEYIDCEYQNKLSKRALAFFN
                     KNKERDLAIIALLLASGVRLSEAVNLDLKDINLNVMVIDVTRKGGKRDSVNVASFAKP
                     YLANYLDIRKNRYKAENQDIALFLSEYRGVPNRIDSSSVEKMVAKYSQDFKVRVTPHK
                     LRHTLATRLYDATKSQVLVSHQLGHASTQVTDLYTHIVNDEQKNALDKL"
BASE COUNT          890 a          421 c          472 g          911 t
ORIGIN      
        1 accgatagac taaaaaattt gttatactaa atgaaagaaa gagatttcgt actattttag
       61 gaggggatat gtcacaagag caaggaaaaa tttatattgt agaagatgat atgacgattg
      121 tgtcactttt aaaagatcat ttatcagcta gctatcatgt ctctagtgtc agcaattttc
      181 gtgatgtgaa acaagaaatt atcgcatttc aacccgattt gatactaatg gatattacgt
      241 taccctattt taatggtttt tactggactg cagaattgcg taagttttta acaattccta
      301 ttattttcat ttcatctagt aatgatgaaa tggatatggt tatggcatta aatatggggg
      361 gtgatgactt tatttcaaaa ccattctctc tagctgtatt agatgctaag ctaactgcta
      421 ttttaaggag aagtcaacaa tttatccaac aggaattaac ttttggggga tttacgttga
      481 caagagaagg gttattgtct agccaagata aagaggttat tttatcgcca acagaaaata
      541 aaatcctatc tatcttgctc atgcatccta aacaagtagt ctcaaaagag tctctattag
      601 agaaactttg ggaaaatgat agttttattg atcaaaatac acttaatgtt aatatgacac
      661 gcttacgtaa aaaaattgtc ccaataggtt ttgattacat tcatacagtg agaggagttg
      721 ggtatttact acaatgatac gccagttttt aagagaacac ttgatttggt atattttata
      781 tatcatgatg tttgtcctat tttttattag tttctatcta tatcatttac caatgcccta
      841 tttgtttaat tccttaggtt taaatgttat tgttttacta ggaattagta tttggcaata
      901 cagtcgttac aggaaaaaaa tgttacatct caaatatttt aatagtagtc aggacccctc
      961 tttcgaactt caaccgagtg attacgctta ttttaatatt attacacaat tagaagctag
     1021 agaagcgcaa aaagtttctg aaacaattga acaaaccaat catgttgcac ttatgataaa
     1081 gatgtggtcg caccaaatga aagttccatt ggcagctatt tcattaatgg cccagacaaa
     1141 tcatctcgat cctaaggaag ttgaacaaca attattgaaa ttgcaacatt atcttgaaac
     1201 gttgttagca tttttgaaat ttagacaata tcgtgacgat tttcgttttg aagctgttag
     1261 ccttagagaa gtagtagtag aaattataaa atcgtataag gttatttgtc tatccaaaag
     1321 cttatctatc ataattgaag gcgataatat ctggaaaaca gacaaaaagt ggttaacttt
     1381 tgctctttca caggtgctag ataatgccat aaaatattct aatcctgagt caaagataat
     1441 aataagcata ggagaagaga gtattagaat acaagactac ggtatcggca tactcgaaga
     1501 ggatatccct agactttttg aagatggctt tacgggttac aacggtcatg agcaccaaaa
     1561 ggcaacaggc atggggttat atatgacaaa agaagtctta tctagtctga atttgtccat
     1621 ttcggtggat agcaaaatta attatgggac tgctgtttct atacataaat aaagttatgt
     1681 aaaataaaat atttacataa ctttatttat gttactatag tttatctaaa gcgttctttt
     1741 gttcatcgtt aacaatatgg gtatataggt cagtaacttg agtggaagca tgacctagtt
     1801 ggtggctaac taatacttga gattttgtag catcatatag cctagtagca agagtatgcc
     1861 ttagtttatg aggtgttaca cgaaccttaa aatcctggga atacttagca accattttct
     1921 ctacacttga agaatcaata cgatttggca ccccacggta ttctgataaa aatagagcaa
     1981 tgtcctgatt ttctgctttg tatctatttt tacggatgtc taaataatta gcaaggtagg
     2041 gcttcgcaaa gctagcgaca ttgacagagt cccttttccc cccttttcgt gtgacatcaa
     2101 taaccattac attaaggtta atatccttta aatctaagtt tactgcctca gatagacgca
     2161 ctcctgaagc tagtagcaaa gcaataatag ctaaatctct ctctttattt ttgttgaaaa
     2221 aagctaaagc acgtttggaa agtttgtttt gatattcaca atctatatac tctaagaatt
     2281 ctatcgtttc attgcccaaa aataactttt gtttaatatt ttctgcccga gaagctaaag
     2341 tttccttttt ctttttggtt gagacttttt tcatgacatt gcgataaaag taaggctccc
     2401 cgtcagcatt ctcaacctct tcagtaaggt atttgaaaag actagataag gcagatagag
     2461 ttctgttaat ggtggtttga gacaccccat tttgccttgt gttagcattt aataaagggc
     2521 gttctcgtaa atatagtata aaagcttcca tatctttttt agtaaggttt tctaaaactg
     2581 aaagctcaat ttctgcaatg tgatgacaat tagcaacacc agaatccaat aaccattcaa
     2641 gaaaacgtct atattctttt aaatattcgt agagtgttgt gaaactataa ggaa
//
LOCUS       DATRQA010000185         2677 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.185, whole genome shotgun sequence.
ACCESSION   DATRQA010000185 DATRQA010000000
VERSION     DATRQA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.185"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1965)
                     /gene="secA2"
                     /locus_tag="VBD27_001933"
     CDS             complement(<1..1965)
                     /gene="secA2"
                     /locus_tag="VBD27_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO7025907.1"
                     /translation="MKKVVVKPKISILNKLRLQRIQKIVNEINNKKVYYAQLSDKELS
                     HQTLILKNRLSSGESLDDILVDAFAVIREANKRILGLFPYDAQVMGAIALHQGTIAEM
                     KTGEGKTLTATMPLYLNALTSKGAILVTTNDYLAKRDALEMGKVYQFLGMTVGINVFD
                     KKEEADATIKREVYESDITYTTAGALGFDYLVHNLASNKLEQFLRPFHFVIVDEADSV
                     LLDIAQTPLIIAGDPRVQSNLYGITNNFVLTLKENDEYIHKAKDKIVYLTEKGVSYAK
                     QYFNISELYNDEYWELNRHINLALRAHCLYKRDYDYVVKNNEVKLLDNATGRVMEGTK
                     LQSGIHQAIEAKEDVALTNESRAIASITYQSLFNMFPRLSGMTGTGKSSEDELIKTYH
                     LPVIVIPTNFPIKRVDLADKIYVSLPEKLQATIDDVKQRHSKGQPVLLISGTVEIANI
                     YSKLLLREGIAHNTLTADNVVKEAQIIKEAGQKGSVTCATVLAGRGTDIKLGEGVREL
                     GGLAVIGTERMPNLRMDWQLRGRSGRQGDPGISQFYVSLEDELIVSHSPEWVIKYLKK
                     YSRKETSNYYMVPRKKRFFYQIVKNAQLRSEDKGVSSREQTIKFGESLRIQRENIYQL
                     RNELIADSSIVVDGVIKIIQDNFNDIANDKD"
     gene            complement(1962..2507)
                     /gene="asp3"
                     /locus_tag="VBD27_001934"
     CDS             complement(1962..2507)
                     /gene="asp3"
                     /locus_tag="VBD27_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEO7025908.1"
                     /translation="MIKKRIQKVLWTDSSNVYLYGSTIKYLEHNRVFFENLRFSTGKT
                     IKKWLSKTNFQANRTLPELTLLKPNHTYELVSKLTTVPEGCVIIQINFYNRQKEKIGF
                     SIIRDHSSDFIVPDDTYFYDISLIGAGCISVEFCYLELFEVIETQEEKDGIELNSYVE
                     SSLPSELELIRTLIAFSKDKK"
     gene            complement(2500..>2677)
                     /locus_tag="VBD27_001935"
     CDS             complement(2500..>2677)
                     /locus_tag="VBD27_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO7025909.1"
                     /translation="YDRNATPRLIDYFSKTNTLLYASGYAGRHNDNSQSINNWFTSQY
                     KTILSDDFGRVFSD"
BASE COUNT          836 a          525 c          366 g          950 t
ORIGIN      
        1 atccttatca ttagcaatat cgttaaaatt atcctgaata atttttatca caccatcaac
       61 cacaatggaa ctatcagcta ttaattcatt tcgtaattga taaatatttt cacgctgtat
      121 acgcaagctc tctccgaact taatagtttg ctccctagaa gatacacctt tatcttcact
      181 tctgagttga gcatttttaa ctatttgata aaaaaagcgt ttctttctag gtaccatata
      241 gtaatttgaa gtttctttac gagaatattt ttttaaatac tttatcaccc attccggaga
      301 atgagaaaca attaattcat cctctagcga cacataaaat tggctaatcc ctgggtctcc
      361 ttgacgcccc gaacggcccc taagctgcca gtccatacgt aaattaggca tcctttccgt
      421 tcctataact gcaagtcctc ctaattctct aaccccttca cctagtttaa tatctgttcc
      481 acgaccagca agaactgttg cacatgttac tgaacctttt tgtcccgctt cttttataat
      541 ttgtgcctct ttaacaacat tatcagctgt taaggtatta tgggcaatgc cttctcgcag
      601 caataacttt gaataaatgt tagcaatttc aactgttcca gaaattaaca atactggctg
      661 acctttacta tgtctttgtt taacatcatc tatagtagct tgcaactttt ctggtaagct
      721 aacatatatt ttatcggcaa gatctactcg tttgatcgga aaatttgttg gtataacaat
      781 aacaggcagg tggtaagttt ttataagttc atcttcagaa gattttccag ttcctgtcat
      841 cccagacaac cttgggaaca tattaaagag actttgatac gttattgaag caattgccct
      901 agattcattt gtcaaagcaa catcttcttt tgcttcaata gcttggtgaa tccctgattg
      961 aagttttgtg ccttccataa cacgacctgt tgcgttatct aacaatttta cttcattatt
     1021 tttaactaca taatcataat ctcttttata caaacaatga gcacgcaatg ctaggtttat
     1081 atgtctattt aattcccaat actcatcatt atataattct gaaatattga agtactgttt
     1141 tgcgtaactt acacccttct cagtaagata gacaatttta tccttagctt tatggatata
     1201 ttcatcattt tccttaagag tgagtacaaa attattagtg ataccataga gattagattg
     1261 tacacgagga tctcccgcta taattaaggg tgtttgagca atatctaaca aaacagagtc
     1321 tgcttcatca acgataacaa aatgaaaagg tcttagaaat tgttctagtt tattactagc
     1381 cagattgtga acgagatagt cgaaacctaa ggcacctgca gtagtataag taatatcaga
     1441 ttcatatact tctctcttaa tagtcgcatc tgcttcttct tttttatcaa aaacattaat
     1501 accaactgtc atacctagaa attgatatac ttttcccatc tctaatgcat ctcgttttgc
     1561 caaataatca ttggtcgtca ctaagattgc tcccttgcta gttaaagcat tcagatacaa
     1621 gggcatagtt gcagttaatg tttttccttc tcccgttttc atttcagcta tcgttccttg
     1681 atgaagagca atagcaccca taacctgagc atcatatgga aacaaaccta aaattctttt
     1741 attagcctct cttatcacag caaaagcatc aacaagaata tcatctagac tttctccact
     1801 tgaaagtcta ttttttaata ttaaagtttg atggcttaac tccttatctg ataattgagc
     1861 gtagtacact tttttattat ttatctcgtt tacaattttt tgaattcttt gcaatctcaa
     1921 tttatttaga atagagattt tgggtttaac aacaactttt ttcatttttt atccttagaa
     1981 aatgctatca acgttctaat aagttcaagc tctgatggta agctgctttc aacataactg
     2041 tttagttcaa taccatcttt ttcttcttga gtctcaatta cttcaaataa ttctaggtaa
     2101 caaaattcta cacttataca acccgcacca ataagagaaa tatcataaaa atatgtgtca
     2161 tcaggaacta taaaatctga ggagtgatct cgaataatag aaaaacctat tttttctttt
     2221 tgacgattat aaaaatttat ctgaataatc acgcatccct caggcacagt agttaatttt
     2281 gataccaact cataggtatg attaggtttt agtaaggtaa gctctggcaa cgttctatta
     2341 gcttgaaaat tcgttttaga aagccatttt ttgattgttt ttcctgtaga gaatcttaga
     2401 ttctcaaaaa aaacacgatt atgctccaaa tactttatag tcgaaccata aagataaaca
     2461 ttgctactat cagtccaaag caccttctgt attctttttt taatcactaa acactctccc
     2521 aaaatcatca ctgagaattg ttttatactg agaagtaaac caattattta ttgattgact
     2581 attatcatta tgtcgcccag catatccact tgcatacaac agggtgttag ttttagaaaa
     2641 ataatcaatc agccttggag ttgcatttct atcataa
//
LOCUS       DATRQA010000186         2673 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.186, whole genome shotgun sequence.
ACCESSION   DATRQA010000186 DATRQA010000000
VERSION     DATRQA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.186"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            123..1331
                     /locus_tag="VBD27_001936"
     CDS             123..1331
                     /locus_tag="VBD27_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO7025910.1"
                     /translation="MVTFDFKHVQTLHTISQLPISVMSRDKELVQLYGNEDYLLPYYQ
                     FLKHLAIPYNQDITVYEGLFEESFLIFPVCQYLIAIGPFYPYSPDINRQEQLSSRFLE
                     QFSHRSKKEILSYIKLVPCFPTTSIRSLLVSIDAFFQTQFEASCQQVINHLLEESEQI
                     VADPDIVLHLKHTKKNSFQLPTVLNHLNHIIDLVKLGNTQLLKQEINRLPSSSVTSSS
                     IPALRAEKNLTVVYLTKLLELSFEENTDVAKSYALVKHYMALNEEAPDLIDVSRIRCA
                     ALIDFSESLTNKSISDKQQMYNSILHYVDNHLYSKLKVSDIANYLYISDSHLRSVFKK
                     YSDISLQSYILKAKIKEGQLLLQRGVPIGEVAKLLHFYDTTHFLKTFKKYVGISSNEY
                     LTKYRETSCQ"
     gene            1525..2020
                     /locus_tag="VBD27_001937"
                     /pseudo
     CDS             1525..2020
                     /locus_tag="VBD27_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            complement(1995..2250)
                     /gene="mobC"
                     /locus_tag="VBD27_001938"
                     /pseudo
     CDS             complement(1995..2250)
                     /gene="mobC"
                     /locus_tag="VBD27_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
     gene            complement(2260..2397)
                     /locus_tag="VBD27_001939"
     CDS             complement(2260..2397)
                     /locus_tag="VBD27_001939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025911.1"
                     /translation="MIIARENHQVTHEHVSILLTCVQELIVEVNQTQPLSREFREKYM
                     G"
     gene            2530..>2673
                     /locus_tag="VBD27_001940"
     CDS             2530..>2673
                     /locus_tag="VBD27_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO7025912.1"
                     /translation="MSTFKRYDEEFKQSLVNLYQTGKTQSELCKDYRVSTSALAKWIK
                     QYSQ"
BASE COUNT          831 a          543 c          441 g          858 t
ORIGIN      
        1 agtttgctaa ttagcgtgtc tttttagtgt atccacaaga tagttgtttt catcatttta
       61 ttttgtagtc tttacaaacc taaagaaaaa ttctagaaat gataatgaag gcggtctatc
      121 aaatggttac attcgatttt aaacatgttc aaacattgca taccatatct caactaccta
      181 tttcagtcat gtcacgagac aaggaactcg ttcagttata tggtaacgaa gactatctgt
      241 tgccttacta tcagttttta aaacatttag ctatccctta taaccaagat atcactgttt
      301 atgagggtct ttttgaagaa tcattcttaa tttttcctgt ctgtcaatat cttattgcta
      361 ttggaccatt ttatccctat agccctgata tcaataggca agaacagtta tccagtcgct
      421 ttctagaaca attttctcat cgtagtaaaa aagaaatctt atcctatata aagcttgtcc
      481 cttgttttcc tactaccagt atacgtagtc ttcttgtgtc tatcgatgcc tttttccaaa
      541 cacagtttga ggctagttgc caacaagtca tcaatcattt attagaagag tcagaacaga
      601 ttgttgcgga tcctgatatt gttcttcacc taaaacatac taagaaaaac tcttttcagt
      661 tacccactgt tttaaaccat ctcaatcaca ttattgatct cgttaagctg ggtaacactc
      721 aactgttaaa gcaagaaatt aatcgcctcc catcatccag tgttacctca tcttcaatcc
      781 ctgccctaag agctgaaaag aacttaactg ttgtttactt aacaaaatta ctagaattaa
      841 gtttcgagga aaatactgat gtggctaaaa gttatgcgct ggtaaagcac tatatggctt
      901 taaacgaaga ggctcctgat cttattgatg tttcaagaat tcgctgtgca gctcttattg
      961 atttttcaga atccttaacc aataagagca tctctgacaa gcagcaaatg tacaatagta
     1021 ttctccatta tgtggacaac cacctctact ccaaactcaa agtatctgat attgccaact
     1081 acctatatat ctcagattcc cacttacgct cagtttttaa aaaatactct gacatttcct
     1141 tgcaaagtta tattctaaag gcaaaaatta aggagggaca attactactg caaagagggg
     1201 taccgattgg ggaagtagcg aaattattac atttttacga caccacacat tttcttaaaa
     1261 cctttaaaaa atacgtggga atatcttcaa acgaatatct tactaaatat cgtgagacct
     1321 catgccagta acatagcaat ccatgaaggt acggaattat ttcatcgtcc ttttgccttc
     1381 atctgaaacg actaaaaaca gccttctcgt ttaaacggaa gactcttttg ttacctttca
     1441 aaatccactg gcactacttt ttagtctaaa attttcagct aatccagtcc atacaaactc
     1501 tctctttgtg ctataacata ggttatggat aaaattatta aatcaatttc tgcctctggt
     1561 gccttcaggt cttatgtcct cgatagcacc gagactgtta aattggctca ggaaaaacat
     1621 cataccttgt cgtcttcaac agttgctctt ggtcgcaccc tgattgctaa tcaaatatta
     1681 gcggctaatc aaaaaggcga tagcaagatt acggttaaag tcatcggtga ttcttccttt
     1741 ggtcatatta tctctgttgc agataccaaa gggcatgtta aggggtacat ccaaaatact
     1801 ggtgttgaca tcaaaaaacg gccacaggtg aagttttggt tggccctttt atggggaatg
     1861 gtcattttgt gaccatcatc gactatggca caggtaatcc ctatacctca acaacgcctc
     1921 ttattactgg gaaaattggt gaggattttg cttattactt aaccgaatca gagcaacccc
     1981 ccctgctgtt gggcttaacg tgcttctcga tgaaagtgat actgagcctg agaaattaga
     2041 tggctttggt tgatagcacg cgcaatttga ttgatgttat ttccaatccg tgttaatcgt
     2101 gacaaatgac atattgggat taagaagaac tcttctagca tagactgaga aattttgaca
     2161 attgctctta gcaatccgct catttaattg attcaattct gaatctgtta gaaagacttt
     2221 tttgagattg gtacgataac gataaacaat tgcagcctcc taacccatat acttttcacg
     2281 aaactcacga ctgagtggct gtgtttgatt gacttccaca atcaattctt gaacgcaggt
     2341 caataagatt gacacgtgtt catgagtcac ttgatgattc tctcttgcga taatcaaaac
     2401 ttcctgcaca tctcaactaa tctgctcaaa cttttggtat tgccaaaggg tggatgtata
     2461 ctataaaagt agacaaaact ttgtgtgtta caatctgttt taaaagacac aaaaacgaaa
     2521 ggacaaccta tgtcaacatt taaacgctat gatgaagaat tcaaacaatc tcttgttaac
     2581 ctttatcaaa ctggaaaaac tcagtctgaa ctctgtaaag actatagggt atccacttct
     2641 gcgcttgcaa aatggatcaa acagtactct caa
//
LOCUS       DATRQA010000187         2629 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.187, whole genome shotgun sequence.
ACCESSION   DATRQA010000187 DATRQA010000000
VERSION     DATRQA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.187"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            14..84
                     /locus_tag="VBD27_001941"
     tRNA            14..84
                     /locus_tag="VBD27_001941"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Gly,seq:tcc)
     gene            119..192
                     /locus_tag="VBD27_001942"
     tRNA            119..192
                     /locus_tag="VBD27_001942"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:153..155,aa:Ile,seq:gat)
     gene            206..293
                     /locus_tag="VBD27_001943"
     tRNA            206..293
                     /locus_tag="VBD27_001943"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Ser,seq:gct)
     gene            529..1854
                     /locus_tag="VBD27_001944"
     CDS             529..1854
                     /locus_tag="VBD27_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7025913.1"
                     /translation="MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSG
                     QQAAAQNQVTAIKGQVGALESQQSELEAQNAQLEAVSQQLGQEIQTLSNKIVARNESL
                     KKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEADKAALEA
                     KQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAK
                     AQAEEAARKAAEAQAVAEAKAQAESVAKAQAAAQVESATAPTETVQTQPRTEIKPSNL
                     TATSSATTVAKTTATATNEPKVTQPSVVTKAVEAPKAVVSSTPRAVSKPVVRSYDSSN
                     TYPMGQCTWGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWPYDGGGY
                     GHVAVVTSVANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN"
     gene            1978..>2629
                     /locus_tag="VBD27_001945"
     CDS             1978..>2629
                     /locus_tag="VBD27_001945"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO7025914.1"
                     /translation="MSYSNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAESVSVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRQ
                     GLVGDDVVVVSPDHGGVTRARKLAQCLKTPIAIIDKRRSVTKMNTSEVMNIIGNIKG"
BASE COUNT          814 a          452 c          565 g          798 t
ORIGIN      
        1 atataagttt ttagcgggtg tagtttagtg gtaaaactac agccttccaa gctgttgtcg
       61 cgagttcgat tctcgtcacc cgcttacttt acataaaagt aaaaccaagc atttgcttgg
      121 gcgcgtagct caggtggtta gagcgcacgc ctgataagcg tgaggtcggt ggttcgagtc
      181 cactcgtgcc cattaatatt aatttggaga attactcaag aggctgaaga ggacggtttg
      241 ctaaatcgtt aggtcgggta actggcgcaa gggttcgaat cccttattct ccgtaaatat
      301 tataatttta agagtaatta tactcttttt ttatttctct tttagtttca ctatggagta
      361 attatattga tagagtgaat agaagaaggt tatcgttttc tagatttgat tatggttcat
      421 ttagaaatag ttgatttcaa atataaatgt aacaatgacg taatataatt tcgtgaattt
      481 tttggtaaaa tatttaatgt cttatcgtaa aggagtagtt taataaaaat gaaaaaaaga
      541 atattatcag cagtccttgt gagtggagta actcttggaa ctgctgccgt aacagtaaac
      601 gctgatgact ttgactcgaa aattgctgct actgattcag taatcaatac tttatcaggg
      661 caacaagcag cagcacaaaa ccaggtgact gctattaaag gacaagttgg tgctttagag
      721 tctcaacaat cagaattaga agcgcaaaat gctcaacttg aggcagtatc tcagcagtta
      781 ggtcaagaga ttcaaactct ttctaataaa attgttgcac gtaacgaatc tttgaaaaag
      841 caagttcgta gtgctcaaaa gggtaacttg actaactata ttaatactat tttaaattcg
      901 aaatcagtat ctgatgctgt taatcgtgtt gttgctattc gtgaggttgt ttcagctaat
      961 gaaaagatgt tagcacaaca agaggctgac aaagcagcct tagaagctaa acaaattgaa
     1021 aatcaaaatg ctataaatac tgtagcagct aacaagcaag ctatcgaaaa taataaagca
     1081 gccttagcaa cacaaagagc acaattagaa gcagctcaat tagagttatc tgctcaattg
     1141 actacagtcc aaaatgaaaa agcatcttta attcaggcaa aagcacaagc tgaggaagct
     1201 gcgcgtaagg cagctgaagc acaagcagta gctgaagcga aagcacaagc tgagtctgta
     1261 gcaaaagcac aggcagcagc acaagttgag tctgccacag ctcctactga aacagttcaa
     1321 acacaaccga gaactgaaat aaagccttct aatcttacgg ctacttcttc ggcaactact
     1381 gttgctaaaa ctacagctac agctactaat gagccaaaag ttactcaacc ttctgtagta
     1441 actaaagcag ttgaagcacc taaagcagtt gtttcttcaa cacctagagc ggtatcaaaa
     1501 cctgttgtca gaagttatga ttcaagtaac acatacccaa tgggacaatg tacttgggga
     1561 gctaagtcaa tggcttcttg ggttggtaac tattggggaa atgctaatca gtggggtgct
     1621 agtgcacgtg cagctggtta ttctgtagga actacccctc gtgttggtgc agtagctgtt
     1681 tggccatatg atggtggtgg ttacgggcac gttgcagtag taacgtcagt ggctaacaat
     1741 tcatctatcc aagttatgga atcaaactat gctggaaata tgagcattgg caattaccgt
     1801 ggttcattta accctagtgc ttctggctct gtttactata tttacccaaa ttaatgaata
     1861 ctaaggagat ctttcgtggt ctcctttttg tttattaatt aggtatggtt tattttggat
     1921 tgaaaaattg tgtaaaaagc gttaaaatgg tataggacta aagttttgga ggaaatcatg
     1981 tcttattcta atttaaaatt gtttgcactg tcatcaaaca aagaactagc gaaaaaagtg
     2041 tctcagacga ttggaattcc gctaggtcaa tcgacagttc gccaattttc tgatggtgag
     2101 attcaagtta atattgaaga gtctattcgt ggccatcatg tttttatttt acaatcaaca
     2161 agctcaccag ttaatgataa tttgatggaa attttaatta tggttgatgc tttaaagcgt
     2221 gctagtgcgg agtctgttag tgtggtaatg ccttactatg ggtatgctcg tcaagatcgc
     2281 aaggcacgtt ctcgtgaacc tattacttcg aaactcgttg cgaacatgct agaagttgct
     2341 ggtgttgatc gtcttttaac agttgattta catgcagctc aaattcaggg attctttgat
     2401 attcccgttg atcaccttat gggggctccc cttattgcag attactttga tcgtcaggga
     2461 ttagttggtg atgatgttgt tgtcgttagt cctgatcatg gtggcgtaac acgtgcacgt
     2521 aagctggcac agtgtttaaa gacaccgatt gctatcattg ataagcgccg tagtgttact
     2581 aaaatgaata catctgaagt aatgaatatt attggtaata tcaaaggta
//
LOCUS       DATRQA010000188         2601 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.188, whole genome shotgun sequence.
ACCESSION   DATRQA010000188 DATRQA010000000
VERSION     DATRQA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.188"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..153
                     /locus_tag="VBD27_001946"
     CDS             <1..153
                     /locus_tag="VBD27_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO7025915.1"
                     /translation="QQLGIKLPSNIADIANTAVTLLVLLGVVTDPTTEGLSDSEQALT
                     YHEPKK"
     gene            279..1622
                     /locus_tag="VBD27_001947"
     CDS             279..1622
                     /locus_tag="VBD27_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEO7025916.1"
                     /translation="MATYQEYKSRSNGNAYDIDGSFGAQCWDGYADYCKYLGLPYANC
                     TNTGYARDIWEQRHENGILNYFDEVEVMQAGDVAIFMVVDGVTPYSHVAIFDSDAGGG
                     YGWFLGQNQGGANGAYNLVKIPYSATYPTAFRPKSFKNAVTVTGNTGLNKGDYFIDVS
                     AYQQADLTTTCQQAGTTKTIIKVSESIAWLSDRHQQQANTSDPIGYYHFGRFGGDSAL
                     AQREADLFLSNLPSKKVSYLVIDYEDSASADKQANTNAVIAFMDKIANAGYKPIYYSY
                     KPFTLNNIDYQQIIAKYPNSIWIAGYPDYEVRKDPLWDYFPSMDGVRWWQFTSVGVAG
                     GLDKNIVLLADDSSKVDIPKIDKPQEPQSQLTFNQKLDTNTKLDNSNVPYYEATLSTD
                     YYVESKPNASSADKEFIKAGTRVRVYEKVNGWSRINASQSDQWVEDKYLANATQV"
     gene            1846..>2601
                     /locus_tag="VBD27_001948"
     CDS             1846..>2601
                     /locus_tag="VBD27_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7025917.1"
                     /translation="MNNLVLPQNLNKYNITKIVTNFNRLLALSDNRTLTVDMRNIEFA
                     EPSGVISLYNMLTFATKRKDANIKWLICEESSLNKRQRQAMLYLVDCGFFKVFDKLVY
                     KEPELRPTTFEIKFINTEQIAQWKVTDFKNWLQKQTGRTNEFSSICVAVDEIFNNIAD
                     HSKESKGCIFGQYYPKNKEIVIAVSDFGIGIPQSIKRKFKKDEPDNKLIEFALQEGVS
                     AETIPQNRGAGLSNIVNTLTTNKVGNFTIISNCG"
BASE COUNT          907 a          435 c          523 g          736 t
ORIGIN      
        1 caacaattag gtattaaatt accgtcaaat attgcggata ttgcaaacac agcagtaacg
       61 cttttggtat tacttggagt tgttacagac ccaacaaccg aaggtctttc agacagtgag
      121 caagcattga cttaccacga gccaaaaaaa taggagggga catgcgtgca atcacaaaaa
      181 tagccttagt actagcaata gcaatattat atattccgtt gtcagtgatt gcttttttta
      241 tttatccgtt ttatttgatt tttaaagagg agggataaat ggctacatac caggaatata
      301 aaagtcgttc aaatggcaat gcttacgata ttgatggatc gtttggtgcg caatgttggg
      361 atggttatgc agactactgt aagtacctag gactgccata cgcaaactgt acaaatacag
      421 gatacgcaag ggacatatgg gagcaacgtc acgaaaatgg tattttaaac tactttgatg
      481 aagtggaagt tatgcaagct ggtgatgttg ctatttttat ggttgttgac ggtgtaacgc
      541 cttacagtca tgtagcaatt tttgacagcg atgcaggagg cggatatggc tggtttttgg
      601 ggcaaaatca aggcggtgct aatggcgcat acaatcttgt aaaaatccca tactccgcaa
      661 catacccaac tgcatttaga ccaaaaagct ttaaaaacgc tgttactgta accggtaata
      721 ctggtttaaa taaaggtgat tactttatcg atgtatcagc ttatcaacaa gcagatttaa
      781 caacgacttg tcagcaggcg ggcactacaa aaacgattat caaggtatcc gagtcaattg
      841 cttggctgtc tgacagacat caacaacaag caaacacaag cgacccaatt ggctattacc
      901 actttggacg ttttggcggt gatagtgctt tagcgcaacg ggaagcagac ttatttttgt
      961 ctaacttacc aagcaaaaaa gtatcatact tagtcattga ctatgaagat tccgcaagcg
     1021 cagacaagca agctaacaca aatgcagtta ttgcgtttat ggataaaatt gcaaacgctg
     1081 gatataagcc tatttattac agctataaac catttacgct taataatatt gattatcagc
     1141 aaattatcgc taagtaccca aacagcattt ggatagctgg ttatccagac tacgaagtac
     1201 gaaaagaccc actgtgggac tattttccat caatggacgg cgtgcgctgg tggcagttta
     1261 caagtgtagg agtagcaggt ggtttagata aaaatattgt attattagca gatgatagta
     1321 gcaaagttga tatacctaag attgacaaac cacaagaacc acaaagccag cttactttta
     1381 atcaaaagct agatactaac actaaattag acaactcaaa tgtaccttac tacgaagcaa
     1441 cccttagcac agactattat gtagagtcta agccaaacgc aagtagcgct gataaagaat
     1501 ttatcaaagc aggaactcgc gtaagagtct acgaaaaagt gaatggatgg tcacgcatta
     1561 atgcttctca gtctgaccaa tgggtagaag ataagtattt agctaatgcc acacaagtat
     1621 aaactaggag gtaaaactcc tttagagcgg aaaccgtatc agtatttggg gcaaattaaa
     1681 ttagtgtaac cgacatcaat gtcggtagca aaaaattatg gaggtaaagc tcctttagat
     1741 aagacaaatg ccctcgcaaa agcgagggct atttttattg aaatattgaa atctctttat
     1801 aaaaatagta aaatagtttc gctattataa agaaagttgt tatcaatgaa taatctagtt
     1861 cttcctcaga atttaaacaa atataacatt acgaaaatcg ttaccaattt caatagatta
     1921 cttgctttaa gtgataacag gacacttaca gtagatatga gaaacattga gtttgcggaa
     1981 cctagtggag taatttcgtt atataatatg ttaacttttg ctacaaaaag aaaagatgca
     2041 aacatcaagt ggttaatatg cgaagaaagc tctttaaata aacgtcaaag gcaagctatg
     2101 ttgtatctag tagattgtgg cttttttaaa gtgtttgata aattggttta taaagagccg
     2161 gaactgcgcc cgactacttt tgaaattaaa tttattaaca ctgaacaaat agctcaatgg
     2221 aaggtaacag actttaagaa ttggttacaa aagcaaactg gcagaacaaa tgagtttagt
     2281 tctatttgtg tagcggttga cgaaattttt aataatattg cagatcattc taaggaatct
     2341 aagggatgta tttttgggca atactatccc aagaacaagg agattgtaat agcagtatct
     2401 gatttcggaa taggaatccc tcagtctata aagcgaaaat ttaaaaagga tgagcctgac
     2461 aacaagttaa tagaattcgc tcttcaagag ggtgtttctg cagaaactat acctcaaaat
     2521 agaggggcag ggctttctaa tattgtaaat actttaacta ctaacaaagt cgggaacttt
     2581 acaattatat ctaattgtgg t
//
LOCUS       DATRQA010000189         2590 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.189, whole genome shotgun sequence.
ACCESSION   DATRQA010000189 DATRQA010000000
VERSION     DATRQA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.189"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            1..858
                     /locus_tag="VBD27_001949"
     CDS             1..858
                     /locus_tag="VBD27_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEO7025918.1"
                     /translation="MKNISPLRYPGGKSQVYDFVKELVNLNETNTYIEPYMGGMGIAL
                     KLLVNNDVKQIMVNDYDRSIYAFWYSVLNHTNQLIDKIESTPITIEEWKKQRKIQQGK
                     ETCHDLLTLGFSTLFLNRTNRSGIIKAGVIGGLKQDGNYKLDCRFNKEKTIKKIRLIA
                     SYKKQIKLYNIDAEKFIRLNITKTKKSFTFFDPPYYSKGPGLYTNFYNHENHLSLSNT
                     IKNYMSNKNWILTYDLSEEIFQMYKEFKYEKYYLNYSVTKPSKGIEYIFYSNGLTVPE
                     NTINIKKAN"
     gene            978..2120
                     /locus_tag="VBD27_001950"
     CDS             978..2120
                     /locus_tag="VBD27_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7025919.1"
                     /translation="MWSEKHKSGKVNFIERYKDPYTSRWKRTSVLMEKDTPRIRKEAQ
                     RILDAKIADIVRKLQTSDMLFTDLLDQWWGFYQQEIKRSSIASLRGNIRDIRESFGID
                     IKVVNIDPKYVQTYLDNLDCSRNKKERNKSMLNLIFDYAVSLDIIKDNPARRAKLPRI
                     KKTLEDWKKIEEKYLEEEEIKRLLKELFRRPSTYRLGLLSEFMSLNGCRIGEAISIEP
                     DNIDFKNKTLQLHGTYDRTNGYMNGEKTSPKTLASYRETIMTKREMEILQEMEFINEL
                     EKNTNKRYRDMGYIFTTKNGIPVQINSFNLALKKANKRLKHPINKNITSHIFRHTLVS
                     RLAENNVSLKAIMDRVGHADAKTTVQIYTHITKKMKSNVADIMENY"
     gene            complement(2209..2484)
                     /locus_tag="VBD27_001951"
     CDS             complement(2209..2484)
                     /locus_tag="VBD27_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO7025920.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFAAVADYLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
BASE COUNT         1013 a          425 c          411 g          741 t
ORIGIN      
        1 ttgaaaaata taagcccgtt gagataccct ggaggtaagt ctcaagttta tgattttgtt
       61 aaagaacttg ttaaccttaa tgaaacaaat acatatatag aaccgtatat gggaggtatg
      121 ggaattgcat taaagctact tgttaataat gatgtaaaac aaatcatggt aaatgactat
      181 gatcgttcaa tctatgcttt ctggtactct gtcttaaatc atacaaatca gttaattgat
      241 aaaatagagt ccacacctat cacaattgaa gaatggaaaa aacaaagaaa aattcaacag
      301 ggtaaagaga catgtcatga tttattaact cttggatttt caactctatt tcttaatcga
      361 acaaatcgtt ctggaataat aaaagcaggt gtcatcggtg gactaaaaca agatggaaat
      421 tataaattag attgtcgttt caacaaagaa aaaactataa aaaaaataag attgattgca
      481 tcttataaaa aacaaattaa actttataat atagatgctg aaaagttcat tcgtttaaat
      541 atcaccaaaa caaaaaaatc tttcactttt tttgaccctc catactactc aaaaggacct
      601 ggattatata caaactttta caatcatgaa aatcatctaa gtttatccaa cacaattaag
      661 aattatatgt caaataaaaa ttggattctc acgtatgatt tatctgaaga aatttttcaa
      721 atgtataaag aatttaagta tgaaaaatat tacctgaact attcagtaac aaaaccaagt
      781 aaaggaatag agtatatttt ctactctaat ggattaacag tcccagaaaa tacaataaat
      841 ataaaaaaag caaattaaaa aaagacctac acagcgccgg caagcaaacg tgtaggtcaa
      901 gttggtaata gtaaaaacct gctttaagta ggctctttac tgtacccatt ttaacaaaaa
      961 aaatgaggta aaaaacaatg tggtcagaaa aacataaaag cggaaaagtg aattttatcg
     1021 aaagatataa agatccgtat acaagcagat ggaaacgtac atcagtactg atggaaaaag
     1081 atactcctcg aattagaaaa gaagctcaaa gaatattaga tgctaaaata gctgacatcg
     1141 taagaaagtt acaaacctcc gacatgctat ttacagacct tcttgaccaa tggtggggat
     1201 tttaccaaca agagataaaa cgctcctcta tagcctctct gagaggcaat ataagagata
     1261 ttagagaaag ctttggaatt gatattaagg ttgtcaatat cgaccctaaa tatgtccaaa
     1321 cctacttgga taacttagat tgctccagga acaaaaaaga gcgaaacaag tccatgctta
     1381 atcttatctt tgattatgct gttagtctag atattatcaa agataatcct gcaaggcgtg
     1441 ctaaacttcc gcggataaag aagactttgg aagattggaa aaagattgaa gaaaaatatc
     1501 ttgaagaaga agaaatcaaa cgactattaa aagaattatt tagaagacct agcacttacc
     1561 gtctgggatt actatcagaa tttatgagtc ttaacggatg ccgtatcggt gaggctatta
     1621 gcatagagcc ggataatatt gattttaaaa acaaaacttt acagttgcat ggtacttatg
     1681 atcgtaccaa tggttacatg aatggagaaa agacctctcc aaaaacgctt gcgtcctatc
     1741 gcgaaaccat tatgactaag cgagaaatgg aaattttaca agagatggaa tttattaatg
     1801 agctcgaaaa aaatacaaat aaacgatata gagacatggg atatatcttt acaaccaaga
     1861 atggtattcc tgtacaaata aattccttta atttagcatt gaaaaaagca aacaaaagac
     1921 ttaagcatcc aatcaataaa aatatcacta gtcacatctt ccgccacaca ttagtcagtc
     1981 gtcttgctga aaataacgta tctttaaagg caatcatgga tcgtgtagga cacgctgacg
     2041 caaaaactac agtacaaatt tatacacaca taaccaagaa aatgaaatca aatgtagctg
     2101 atatcatgga aaactactaa tttcatgccc caaatatgcc ccaaaaataa aaaaaagaac
     2161 cttgtcgcta tcaataaaca ttgatatgac aaggtttttc tagacgaatt atttaacagc
     2221 gtctttaaga gctttaccag ctttaaatgc tggaactttt gatgctgcaa tttcgatttc
     2281 tgcaccagtt tgtgggttac gacctttacg agctgcacgt tcacgaactt caaagttacc
     2341 aaaaccgatt aattgtactt tttcaccttc agcaaggtaa tctgctacag ctgcaaaaac
     2401 tgcatctact gctgctgctg aatctttttt agttaattct gtagcttctg ctacttttgc
     2461 aattaaatct tgtttgttag ccatgttaac aaatcctcca aataaattct aaacttatac
     2521 aacataagtt ttaacagatt atatgatatc taaaaaaagg cttaaggtca agtatttaac
     2581 gtcattttag
//
LOCUS       DATRQA010000190         2589 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.190, whole genome shotgun sequence.
ACCESSION   DATRQA010000190 DATRQA010000000
VERSION     DATRQA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.190"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..196
                     /locus_tag="VBD27_001952"
     CDS             <1..196
                     /locus_tag="VBD27_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEO7025921.1"
                     /translation="IPNQLLPFLVPLIMLQGALIIISLVKLSKLKMTKHLSKPVWFLV
                     IIFLNIIGPIAFLILEGNNE"
     gene            193..1095
                     /locus_tag="VBD27_001953"
     CDS             193..1095
                     /locus_tag="VBD27_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025922.1"
                     /translation="MKAISFQNVTKSFGPKKILNNVSFDLEENMIYGFVGPNGAGKTT
                     TIKMILGLLKFDTGFITIFGKKVNFGRTDTNQLIGYLPDVPEYYDYMTALEYLDLCSG
                     LARSKHKLSNKELLRSVGLDDNHQKIATYSRGMKQRLGLAQALVHDPKIIICDEPTSA
                     LDPKGRQDILDIISNLRGEKTVIFSTHILSDVEKICDHVLVLTKCGIYSLEELKGKKS
                     EENYSVRILIKVTKSEAKVLSHNYQIEKKDNEYALTLKGSKMDNKADLLAGFYQDLVS
                     LKISPSAIEVIDNSLEELYLEVTK"
     gene            1092..1853
                     /locus_tag="VBD27_001954"
     CDS             1092..1853
                     /locus_tag="VBD27_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025923.1"
                     /translation="MKSLAVMLKKEWMENVRTYKVISILITCSIFGILGPLTALMMPD
                     IMAGILPKKLQGAIPETTYIDSYIQYFKNMNQLGLVILVFLFSSTLTQEFSKGTLINL
                     VTKGLAKKVIILAKFIVITLLWTVSYLLSVVIHFSYTLYYFSNEGSHKLMVYGATWFI
                     GILFISLILFFSVLFRKTLGILLGCLFMVVLCVVSGFFKISKSYNPMLLIQKQGEMLS
                     GKFKVQDLMEPSILVFLLIIMTLLLSMYIFEKSEV"
     gene            2022..2510
                     /locus_tag="VBD27_001955"
     CDS             2022..2510
                     /locus_tag="VBD27_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7025924.1"
                     /translation="MEIKEYENNPYHLAQLVDLINYCQNIEAKLDIKMAEQDDIFQIE
                     NYYQNRKGQFWIALENEKVVGSIALLRIDDKTAVLKKFFTYPKYRGNPVRLGRKLFER
                     FMLFARASKFTRIVLDTPEKEKRSHFFYENQGFKQITRDKLDVDYIFPDRDSRIYVKL
                     LD"
BASE COUNT          926 a          350 c          436 g          877 t
ORIGIN      
        1 aataccaaat caattattac cattcctagt tccgttgatt atgttgcaag gagctttaat
       61 tatcatttct ctagtcaaac taagtaaact taagatgact aaacaccttt caaagccagt
      121 ttggtttttg gtcattatat ttcttaatat aattgggcca attgcatttc ttattttgga
      181 agggaataat gaatgaaagc tatctcattt caaaacgtta caaaatcttt tggtcctaaa
      241 aaaatattaa ataatgtttc ttttgattta gaagaaaata tgatttatgg attcgtagga
      301 ccaaatggag caggtaaaac aacgacaata aaaatgattt taggattgtt aaaatttgat
      361 acgggtttca taacaatttt tggtaaaaaa gttaattttg gaagaacaga taccaatcaa
      421 ctgattggct atctcccaga tgttcccgag tactacgatt atatgacagc tctagaatat
      481 cttgatttat gttctggttt agcgagatca aagcataagc tttctaacaa agaactactt
      541 aggtcagttg gacttgacga taatcaccaa aagattgcta cttattctcg aggaatgaaa
      601 caacgtcttg gattagccca agcactggtc catgatccta aaattattat ttgcgatgaa
      661 ccaacctctg cattagaccc gaaaggtcgc caagatattt tagatattat ttcaaatctt
      721 agaggagaaa aaactgtgat tttttctacc catattctct ctgatgtcga aaaaatatgt
      781 gatcacgttc tggtgttaac taagtgtggg atttattctc ttgaagagtt aaaaggaaaa
      841 aaatcagagg aaaattattc ggttcgtatt ttaataaaag tgacaaagtc tgaagctaag
      901 gtactatctc ataactatca gatagaaaag aaagataatg agtatgcact gactctcaaa
      961 ggctctaaaa tggataacaa agcagattta ctagctggtt tttaccaaga tttagtttct
     1021 ttaaagatta gcccatcagc tattgaagtc attgacaata gtttagagga gctttatttg
     1081 gaggttacca aatgaaatct ttagcagtaa tgttaaaaaa agaatggatg gagaatgtta
     1141 ggacatacaa agttatatct attctgatta cttgttcaat atttggtata ttaggtccct
     1201 taactgcact tatgatgcct gatattatgg caggaatttt acctaaaaaa ctacagggag
     1261 cgataccaga gactacctat attgattctt atattcagta ttttaaaaat atgaatcagc
     1321 ttggtttagt tattttagta tttttattta gtagtacact gacgcaagaa ttttcaaaag
     1381 gaaccttaat taatctagtt acgaagggat tagccaaaaa agttataatc cttgctaaat
     1441 ttatagtaat tacccttcta tggacagtgt catacttatt aagtgttgtc atccatttta
     1501 gttatacgtt atattacttt agtaacgaag gaagtcataa attaatggtt tacggtgcaa
     1561 catggtttat cggtattctt ttcataagct taatactctt tttttcagtt ctttttcgta
     1621 aaactttagg gattttgtta ggttgtcttt ttatggtggt gttatgtgta gtatctggat
     1681 tttttaaaat cagtaaaagc tataatccta tgcttcttat ccaaaaacaa ggagaaatgt
     1741 taagtggaaa gtttaaagtt caagacttaa tggaaccgag catattagtg tttttgctta
     1801 ttataatgac gttattactt agcatgtaca tttttgaaaa aagtgaagtt taattttgat
     1861 aagatatcaa taaggcgata gctacttttt gaaaaaataa aaccgaatta agagctttgc
     1921 tgctatttga cttatctaga tcaatatttc caactaatat gatatcctat gcatatctca
     1981 ttgcagaaat atactttaaa gtcaaaataa ggaaatatat tatggaaatt aaagaatacg
     2041 agaataatcc gtaccattta gcacagctag tagatttaat taattattgt cagaatatag
     2101 aagctaaact tgatataaag atggctgaac aagacgatat ttttcaaata gaaaattatt
     2161 atcaaaacag aaaaggtcaa ttttggattg cattagaaaa tgaaaaagta gttggttcga
     2221 ttgcgttact gcgtattgat gataaaacag ctgttctaaa aaaatttttt acatatccaa
     2281 aatatagagg aaatcctgtt cgactcggtc gaaaattatt tgaaagattt atgctttttg
     2341 ctcgagctag taaattcaca agaattgtct tggatactcc agaaaaagag aaaagatctc
     2401 acttctttta tgaaaatcaa ggatttaagc aaataacaag agataaacta gatgtagact
     2461 acatttttcc agatagagat tcaaggatat atgtaaaact tcttgattaa ttgtgaagaa
     2521 aactagccaa acagatgaat aaacccaaat aacgattatt ttagataata cattggaaaa
     2581 gtggttgta
//
LOCUS       DATRQA010000191         2529 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.192, whole genome shotgun sequence.
ACCESSION   DATRQA010000191 DATRQA010000000
VERSION     DATRQA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.192"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            106..951
                     /locus_tag="VBD27_001956"
     CDS             106..951
                     /locus_tag="VBD27_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEO7025925.1"
                     /translation="MLSVGLVLEGGGMRGLYTAGVLDAFLDAGIKIDGIVSVSAGALF
                     GVNFVSRQRERALRYNKKYLSHPEYMSLRSWLRTGNFVNKDFTYYEVPMKLDVFDDEA
                     FKKSSIDFYVVATEMTSGKPEYFKIDSVFEQMEILRASSALPVVSKMVDWQGKKYLDG
                     GLSDSIPVDFARGLGFDKLIVVMTRPLNYQKKPSSGRLYKTLYRKYPNFVKTASNRYQ
                     QYNNSLEKVMSLEKTGDLFAIRPSKSLVIGRLEKNPDKLDSIYQLGMKYAKSVMPELN
                     SYLMK"
     gene            complement(994..2241)
                     /locus_tag="VBD27_001957"
     CDS             complement(994..2241)
                     /locus_tag="VBD27_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7025926.1"
                     /translation="MSPLNINKNNWRALIAAIVASGTDDLNIMFLAFSMSTIITDLHL
                     SAAQAGWIGTITNLGMLVGGLIFGLLADRYNKFKVFKWTILIFSIATGLVFFTTNLSY
                     LYIMRFIAGIGVGGEYGIAIAIMAGIVPANKMGRISSLNGIAGQVGSISSALLAGWLA
                     PALGWRGLFLFGLLPIVLVLWMQFAIDDKDILDQYNTDADDEPLDISIKALFDTPVLA
                     TQSLALMVMTTVQIAGYFGMMNWLPTIIQTNLNVSVKNSSLWMIATIFGMCLGMLVFG
                     QLLDKFGPRLVYGCFLLSSAICVYLFQFATTMPSMIIGGAVVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNLILNVGRAIGGFSSVIIGMILDVSNVSMVMLFLASLYIVSFLSML
                     SIKQLKRQKYHTNLTQLDVKPTD"
BASE COUNT          851 a          410 c          511 g          757 t
ORIGIN      
        1 taaattaatt atggagttta tatagtacaa catattcttt ttcagaaaaa acagctatat
       61 tttgctatac tttaatcaaa tatcaatatt agaaagtgat tgcctatgtt gtctgttggt
      121 ttagttttag agggtggcgg aatgagaggt ctttatactg ctggagtttt agatgctttt
      181 ctagatgcag gaataaaaat agatggtatc gtatctgtct ctgcgggtgc attgtttggt
      241 gttaattttg tatctagaca acgagagagg gctttgcgat acaataaaaa gtatttatcc
      301 caccctgaat atatgagtct aagatcatgg cttcgaacag ggaattttgt taataaagat
      361 ttcacctatt atgaagttcc tatgaaattg gatgtatttg acgatgaagc atttaaaaaa
      421 tcaagtattg atttttacgt agttgctaca gagatgacat ctggtaaacc tgaatatttt
      481 aaaattgata gtgtttttga acaaatggaa attttacgtg ctagttcagc attaccagta
      541 gtctcaaaga tggttgattg gcaggggaaa aagtacttag atggtggttt atctgatagt
      601 attcccgttg attttgcccg tggtttagga tttgacaagt tgattgttgt gatgactagg
      661 ccgctcaatt atcagaaaaa gccttcaagt ggacgattgt ataaaactct gtataggaaa
      721 tatcctaatt ttgtaaagac agcctcgaat cggtaccaac agtataataa tagtcttgaa
      781 aaggtcatga gccttgaaaa aacaggcgat ctatttgcaa ttagaccgag taagagcttg
      841 gttattggcc gcttagagaa gaatccggat aaacttgata gtatttatca gcttggtatg
      901 aaatatgcta aaagtgtgat gcctgagctg aatagttatc taatgaaata aagaaaacag
      961 tatttctcaa ttgaggaata ctgttttgta atatcagtca gttggtttga catctaattg
     1021 tgttaaatta gtgtgatatt tttgacgttt taattgctta atgcttagca ttgataagaa
     1081 actaacgata tagagacttg ctaagaaaag catgaccata gagacatttg aaacgtctag
     1141 aatcattccg atgataacag acgaaaagcc acctattgca cgaccaacat ttaagataag
     1201 attgttagct gttgaacgaa tatgatgtgg atagagacgt gtaatcatgg caccatagcc
     1261 tgcaaacata ccattaacaa agaagccgac aactgccccg cctataatca tagaaggcat
     1321 tgttgttgca aattgaaaga gatagacaca aattgcagat gatagaagga aacacccgta
     1381 tactaaacgc ggtccaaatt tatctaacaa ttgaccaaat actagcatgc caagacacat
     1441 tccaaaaatg gttgcaatca tccataatga tgaattcttg acagaaacgt ttaagttggt
     1501 ttggataata gttggtaacc agttcatcat gccaaagtat ccggcaatct gaacagttgt
     1561 cataaccatt agtgctaggc tctgtgtggc taatacagga gtatcaaata aagcttttat
     1621 actgatatct agaggttcat catctgcgtc tgtattatat tgatctaaga tatctttatc
     1681 atcaatagca aattgcatcc ataggacgag aactatagga agaaggccaa agagaaaaag
     1741 tccccgccaa ccgagtgctg gtgctagcca tcctgctaag agtgctgagc taatagatcc
     1801 gacttgacca gcgataccat ttagtgaaga aatccggccc attttattag ctgggacaat
     1861 tcctgccata atggcaatag cgataccata ttcgccacca acgccaattc ctgcaatgaa
     1921 gcgcatgata tagagatatg ataaattagt agtgaagaaa actaaaccag ttgcgattga
     1981 aaagattaga atcgtccatt tgaaaacttt gaatttattg tagcgatcag ctaataaacc
     2041 aaagattagt cctcccacga gcattcccaa gttggtaatt gtcccaatcc aaccagcctg
     2101 tgccgcagat aaatgaagat cagtaatgat agttgacata gaaaatgcaa ggaacatgat
     2161 atttaaatca tcggttccgg atgcgacaat ggcagcgatt agggcgcgcc aattattctt
     2221 attgatattt aatggtgaca taagtctcct cctttaatga tggctcacag accaccgtta
     2281 ttaaatactg tcaatagaaa aaattctaaa gcagagatgt catagctgct tcaactcatt
     2341 ataatgtaaa agaaaaaact caagctgcta cccttgagtt ctataaacgc tcgcaaaaat
     2401 agtaggattt ctgtatagaa tcctattttg gtcgcttata tgtctcacag gacacggtta
     2461 aagcattatg tcatttggaa atagtataga gtaagtttag tttattgtca aggataagtg
     2521 gggagagaa
//
LOCUS       DATRQA010000192         2509 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.193, whole genome shotgun sequence.
ACCESSION   DATRQA010000192 DATRQA010000000
VERSION     DATRQA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.193"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            108..560
                     /locus_tag="VBD27_001958"
     CDS             108..560
                     /locus_tag="VBD27_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025927.1"
                     /translation="MSKGYSRQQKKSTKTNTDSTQSPAKHIKTGFSALQKTIALVGSV
                     LSIIVASITITNAINNHKDSSKTDSSTSKTVIIRESGQNSSNQVSTEKDETRASSSQT
                     LENSSASSTPSSSSNSAANNDQTSSSTNNDQTNNTTTDTSTIENTTTP"
     gene            complement(603..1952)
                     /gene="gorA"
                     /locus_tag="VBD27_001959"
     CDS             complement(603..1952)
                     /gene="gorA"
                     /locus_tag="VBD27_001959"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO7025928.1"
                     /translation="MSKQYDYIVIGGGSAGSGTANRAAMYGAKVLLIEGGQVGGTCVN
                     LGCVPKKIMWYGAQVSETLHKYSSGYGFEANNLSFDFTTLKANRDAYVQRSRQSYAAN
                     FERNGVEKIDGFARFIDNHTIEVNGQQYKAPHITIATGGHPLYPDIIGSELGETSDDF
                     FGWETLPNSILIVGAGYIAAELAGVVNELGVETHLAFRKDHILRGFDDMVTSEVMAEM
                     EKSGISLHANHVPKSLKRDEGGKLIFEAENGKTLVVDRVIWAIGRGPNVDMGLENTDI
                     VLNDKDYIKTDEFENTSVDGVYAIGDVNGKIALTPVAIAAGRRLSERLFNHKDNEKLD
                     YHNVPSVIFTHPVIGTVGLSEAAAIEQFGKDNIKVYTSTFTSMYTAVTSNRQAVKMKL
                     ITLGKEEKVIGLHGVGYGIDEMIQGFSVAIKMGATKADFDDTVAIHPTGSEEFVTMR"
     gene            complement(2076..>2509)
                     /locus_tag="VBD27_001960"
     CDS             complement(2076..>2509)
                     /locus_tag="VBD27_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEO7025929.1"
                     /translation="LEQEHSYHDNLKQIFPLLSMKLFRKSLFNNITFPLEDLTGEYTL
                     YNVYLLSRHITFSHIGLYITSQVFSHTEIKRFTGHDIEKMLSRKEERLALLTMVGFNS
                     EKYIKNYKETLLNYQSESLNIGDINLYNKIKQKIALIDYCY"
BASE COUNT          818 a          518 c          364 g          809 t
ORIGIN      
        1 tagcttaata acctaggttg cttatttaaa tgttttaaat tattttttct ttagcttttt
       61 ttaggataaa atcatataat agttgatgta catttaagga gggcgtcatg tcaaaaggat
      121 attcaagaca acagaaaaag tctactaaga ctaatactga ttcaactcaa tcacccgcta
      181 agcatataaa aacaggtttt tctgcacttc aaaaaacaat tgcattagtc ggtagtgttt
      241 taagtattat cgttgcctct attactatta ctaatgcgat taataatcac aaagactcta
      301 gtaagactga ttcttctact tcaaaaaccg tcattataag agaaagtgga caaaattcta
      361 gtaaccaagt ttctactgaa aaagatgaga caagggcatc atccagccaa acactagaaa
      421 attcctctgc tagtagtact ccgagtagca gttcaaattc tgctgccaat aacgatcaaa
      481 catcatctag tactaacaat gatcaaacaa ataatactac taccgatacc tcaactattg
      541 aaaatacaac aacaccttaa aaaagctagg ttccctagct ttttttgtat tctttgatag
      601 cgttagcgca ttgtaacaaa ttcctcagat ccagttgggt gaatagcaac agtatcatca
      661 aagtctgctt tagtagcccc cattttgata gcaactgaaa aaccttgaat catttcatca
      721 ataccataac caacaccatg aagcccaata actttttcct cttttcctag ggttatgagc
      781 ttcatcttaa ctgcttggcg attactggta acagccgtat acatagaggt aaaagttgat
      841 gtatagactt tgatattatc ttttccaaat tgctcgatag ctgctgcttc tgaaagtcct
      901 accgtcccaa ttacagggtg agtaaaaata actgaaggta cattatggta atctaatttt
      961 tcgttatctt tatgattaaa aagtctttct gataagcgac gacctgctgc aattgctacc
     1021 ggtgtcaagg caattttccc attaacatct ccaatagcat acacgccatc tacagaagta
     1081 ttctcaaatt catctgtttt gatataatct ttatcattta aaacaatatc ggtattttca
     1141 agtcccatgt ctacatttgg tccacggccg atagcccata ttacacgatc aacgacaagc
     1201 gttttcccat tttcagcttc aaaaatcaac ttgccacctt catcgcgttt aagagattta
     1261 ggtacatggt tagcatgtaa agagatacct gatttctcca tttcagccat aacctcactt
     1321 gttaccatgt catcaaatcc gcgtagaata tggtcttttc taaatgcaag atgggtttca
     1381 acgcctaatt cattaaccac tccagcaagt tctgccgcga tatagcccgc cccaacaatc
     1441 aatatagaat ttggtaaggt ctcccatcca aaaaaatcat cagaagtctc accaagttca
     1501 cttccaataa tatcagggta aagagggtgt ccacctgttg caatagtaat gtgaggagct
     1561 ttatattgct gaccattcac ttcaatagta tggttatcaa taaaacgagc aaatccatca
     1621 atcttttcga ccccattacg ctcaaaatta gcggcatacg actgtctaga ccgctgcacg
     1681 taagcatcgc gattagcttt tagagtagta aaatcaaaac taagattatt ggcttcaaaa
     1741 ccataacctg aactatactt atggagtgtc tcagaaactt gtgcaccata ccacatgatt
     1801 ttcttaggta cacaacctaa gttaacacaa gttccaccta cttgtccacc ttcaattaac
     1861 aggacttttg ctccatacat ggctgcccta ttagcggtac cactgcctgc actacctcca
     1921 ccaataacga tataatcata ttgttttgac attactctct ccttgaatca ttgataccta
     1981 tactctatca caaagtgttt acagtcacaa ttttctgcta cgtaaacaat ttatattacc
     2041 gattatcata tcctaacttg ctgttaaaat tatttctagt aacaataatc tatcaatgca
     2101 atcttttgct taattttatt atataaatta atatcaccta tattcaacga ctcactttga
     2161 taatttaata aagtttcctt ataatttttg atgtatttct cagaattaaa ccctaccatt
     2221 gttaacaggg ctaacctctc ttcctttcta gatagcatct tctctatatc atgtcccgtg
     2281 aatcttttta tttcagtatg agagaaaact tgagaggtta tgtataaacc aatatgagaa
     2341 aatgtaatgt gccttgatag caaataaaca ttatacagtg tgtattctcc tgttaaatct
     2401 tctaaaggaa aagtgatatt gttaaaaagt gattttctaa agagcttcat agaaaggaga
     2461 ggaaatattt gttttaaatt atcgtggtaa ctatgttctt gctctaacc
//
LOCUS       DATRQA010000193         2494 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.194, whole genome shotgun sequence.
ACCESSION   DATRQA010000193 DATRQA010000000
VERSION     DATRQA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.194"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            170..1275
                     /locus_tag="VBD27_001961"
     CDS             join(170..446,446..1275)
                     /locus_tag="VBD27_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag2 family transposase"
                     /protein_id="HEO7025930.1"
                     /translation="MSRKVRRHFTDDFKQQIVDLYNVGRKRSSLIKVYELTPSTFDKW
                     VRQAKTTGSFKSVDNLTDEQRELIELRKHNKELEMQLDILKQAAVIMAPKREIITANK
                     DKYSISKMCRWLNMPHSSYYYQAVESVSETEFEETIKRIFLDSESRYGSRKIKICLNN
                     EGITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALV
                     TDLTYVRVGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFH
                     SDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLE
                     ELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA"
     gene            1499..1789
                     /locus_tag="VBD27_001962"
     CDS             1499..1789
                     /locus_tag="VBD27_001962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025931.1"
                     /translation="MVKKAYSWETKLACIDMKKAGKSNRVIMETLGIKNNSQIYTWMK
                     WYENEELYRFHQGVGKQYTYGKGLEHLSEVEQLQLQVDLLKKYRGLIRKSIK"
     gene            1947..2126
                     /locus_tag="VBD27_001963"
                     /pseudo
     CDS             1947..2126
                     /locus_tag="VBD27_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2371..>2494
                     /locus_tag="VBD27_001964"
     CDS             2371..>2494
                     /locus_tag="VBD27_001964"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7025932.1"
                     /translation="MAYYQACTEKDIIRSMSRKGTPADNACIEWFHTVLKTETFY"
BASE COUNT          874 a          469 c          475 g          676 t
ORIGIN      
        1 cttgagattt ataccaaagc gaacaaaaga cttggtgtga agtctatcaa ggtcattctt
       61 caaagagact atgacacaaa aatctctgaa ggaagaattt gaataggtta cactaaacta
      121 gacagaaatt ataaagtgtt ctacacttaa gaaaaactgg agaaaaacca tgtctagaaa
      181 agtacgtcgt cacttcaccg atgattttaa gcaacaaatt gttgaccttt acaatgttgg
      241 tagaaagcgt agcagtctca tcaaggtata tgagctaacc ccttccacct tcgataagtg
      301 ggttagacaa gccaaaacaa ctggttcatt caagtctgtt gataatctga cagatgaaca
      361 gcgggagcta attgaactca gaaaacacaa taaagaactc gaaatgcaat tagatatcct
      421 aaagcaagca gcggtgatta tggcacaaaa agggaaataa tcactgctaa taaggataaa
      481 tacagcattt caaagatgtg tcgctggctg aatatgccac actcaagtta ttactatcaa
      541 gccgtggagt cagtatctga aacggagttt gaagaaacta ttaaaagaat tttcctcgat
      601 agcgagtcca gatacggatc cagaaaaatc aaaatatgct tgaataacga aggtatcaca
      661 ctttcacgtc gtcggattcg acgcattatg aagcgactca atttggtttc tgtttatcag
      721 aaagccacct tcaaaccaca ttctagaggc aagaatgaag cccctattcc caaccactta
      781 gacaggcaat ttaagcaaga aagaccacta caggccttag tcactgactt aacctatgtt
      841 cgtgtaggca atcgttgggc ttatgtttgc ctcatcattg acctatacaa ccgtgaaatc
      901 atcggcctgt ctcttggttg gcacaagacc gctgaactcg ttaagcaagc catacaaagc
      961 atcccttacg ccctgaccaa agtcaagatg ttccattcag atcgtggcaa agagtttgat
     1021 aatcagttaa ttgatgaaat attggaagcc tttggaatca cacgttcgct tagtcaggct
     1081 ggttgtcctt atgacaatgc cgtagctgaa agtacgtatc gtgctttcaa aattgaattt
     1141 gtttatcaag aaacctttca attgctggaa gaactagctc ttaagactaa agactatgtt
     1201 cattggtgga attaccaccg cattcatggt agtctcaact accaaacacc aatgactaaa
     1261 agattaattg cctagaaaaa cactttataa atgttgtaca gaaaagtgtt gccttttcac
     1321 ttaataaaag aaagattgaa ggaacacttc tctttcattc cgaccaaggg tcacaattta
     1381 agtccgggga atttagaaaa ataattgatg acaacaatag gcactgaatc cctaaaatga
     1441 gacaaaagaa aaacactcct gttgaaattt agtaaactag aaacaggagt gttttattat
     1501 ggttaaaaaa gcatattcat gggaaactaa gctggcttgc atcgatatga agaaagcagg
     1561 caagtctaac agagttatta tggagacttt aggcatcaaa aataatagtc aaatctatac
     1621 ttggatgaaa tggtacgaaa acgaggagct ttatcgtttt catcaaggtg ttggcaagca
     1681 atatacctac ggtaaaggct tggaacatct gtctgaagtg gaacaattac aattacaggt
     1741 tgacctctta aaaaagtatc gaggcttgat aaggaaatcg ataaagtaag tcttatcaag
     1801 cttgtggaag actataaaaa caactacccg atagttttca ttctagactg ttttaaggtc
     1861 aagcggtcga cctattaccg ttggaaaaag gagtatgaga agccgcaaaa aaagatgacc
     1921 ttattgaatt aattgaacag ctctgtatgg agaatcactt tatttatggc tatcgtacca
     1981 ttacacgatt gctcaaaaaa atccacggat tgactgttaa tacaaagaaa gtctatcgta
     2041 tcatgaaaaa taatggctgg ctttgtcgga cacgtaccaa gaaagttccc aatttgggta
     2101 aagcttatta tttaacagat aataaattga gtcgggattt ccatgccgat aagcctaagg
     2161 aaaagctcgt aacagatatt acctacctat atttcggtaa ctgtaaattg tatctctcat
     2221 caattatgga tctctataac cgagaaatta tagcttacac tatctcagat tgtcaggaca
     2281 cggattttgt actagacacc cttaatcagt taaaattgcc taaataaagg agcaatctta
     2341 cacagtgacc aaggctcagt gtatacttct atggcttact accaggcttg cacggaaaaa
     2401 gacattatcc gctcaatgtc tcgaaaagga acacctgcag ataatgcctg tattgaatgg
     2461 tttcacaccg tcctaaagac tgaaaccttt tatt
//
LOCUS       DATRQA010000194         2356 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.195, whole genome shotgun sequence.
ACCESSION   DATRQA010000194 DATRQA010000000
VERSION     DATRQA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.195"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(68..1402)
                     /gene="trmFO"
                     /locus_tag="VBD27_001965"
     CDS             complement(68..1402)
                     /gene="trmFO"
                     /locus_tag="VBD27_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEO7025933.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEAHRVPAGGAMAVDREGY
                     SEAVTEEIHKHPLIEVIRDEITDIPGDAITVIATGPLTSDSLAAKIHELNGGDGFYFY
                     DAAAPIVDKNTIDMNKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLNQT
                     FATRKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFNGESEVVFPQTTAIGALPH
                     YITHTDSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIATRALKDLEKFLNY"
     gene            complement(1516..2214)
                     /locus_tag="VBD27_001966"
     CDS             complement(1516..2214)
                     /locus_tag="VBD27_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7025934.1"
                     /translation="MLPAYIKIHDAIKKEIDKGTWKIGQRLPSERDLADDYSVSRMTL
                     RQSITLLVEEGILERRVGSGTYVASHRVQEKMRGTTSFTEIVNSQGRKPSSKLISFQR
                     KLANETEIQKLNLSQSDYVVRMERVRYADKVPLVYEVASIPENLIKGFEQSEVTEHFF
                     KTLTENGYEIGKSQQTIYARNASERVASHLEVNAGHAILALTQVSYFTDGKPFEYVHG
                     QYVGDRFEFYLENN"
BASE COUNT          726 a          490 c          362 g          778 t
ORIGIN      
        1 ttcatacatt tttaaaaatc aatatagaaa atttgacctt gcttgttggc aaagtcaaat
       61 gaatatatca atagttgaga aatttttcta aatctttcaa agctcgtgta gcaatagctt
      121 cataacgctc tttcttatca cgaattcgtg gaccttctaa ttctttgatg ataccaaagt
      181 taacattcat tggttggaaa tgtttgctgt ctgtatgtgt aatataatgt ggcagtgcac
      241 caatagcagt agtttgtggg aaaactactt cactctcccc attgaagcga cggacagcat
      301 tgattcccgc tacaagacca cttgctgccg attctacata accttcaaca ccggtcattt
      361 gtccagcaaa gaaaagattt gggttcttac gtgtagcaaa ggtttggttt aaaagatttg
      421 gtgaatccat ataagaattg cgatgcataa caccataccg gacaaattca gcattttcaa
      481 gaccaggaat catttgaaat acacgttttt gttctcccca cttaagatga gtttggaaac
      541 caactatgtt ataaagagag cctgcagcat tatcttgacg taattgaaca acagcatatg
      601 gtgttttaaa ttcaccatct cgcggtcctt tgtagtcctc aggatactcc aaaccaactg
      661 gtttcattgg accataaagc atcgttttaa taccacgctt agccatcact tcgataggca
      721 tacaaccttc gaagtatttt tctttctcaa aagaatttag tggtgcttcc tcagctgtag
      781 ttaaagcttc atgaaaagcc ataaattctt ctttggtcat tggacaattt agataagctg
      841 cttcaccttt gtcataccta gacttaaggt aaactttatt catgtcaatc gtatttttat
      901 cgacaattgg agcggcggca tcgtaaaaat aaaaaccatc accaccatta agttcatgga
      961 ttttagctgc cagactatca cttgttaatg gtcctgtagc gataactgta atagcatctc
     1021 cagggatatc tgtgatttca tcacgaatta cttcgattaa tggatgttta tggatttcct
     1081 cagtaacagc ttcagaataa ccttctctat caacggccat ggctccacct gctggaactc
     1141 gatgtgcctc cccatttctc ataatgatag aatccaaacg acgcatttct tcttttaaaa
     1201 gaccaactgc atttgttaga gaatcaccgc ggaatgaatt ggaacatact aattctgcaa
     1261 aattatctgt tttatgttga ggggttgatt taaccccacg catttcataa agcttaactg
     1321 gtattccacg tttagcaatt tgataagctg cttcagaacc agccaaacca gcgccgataa
     1381 cattaatgta agattgagac atgaactaat acctcttgaa agtagtcaac tacttcctca
     1441 aatctttcta tataatttat ttttccatta taacatacat agctacttct tcaaaaaacg
     1501 ttattcatta aactactagt tattctctaa ataaaactca aaacgatctc caacatattg
     1561 cccatgaaca tattcaaaag gcttaccatc agtaaagtat gaaacttgtg tcaatgccaa
     1621 aatagcatgg cctgcgttaa cttctaaatg cgaagcaaca cgttcactag catttctagc
     1681 atatatagtt tgttggcttt tcccaatttc gtagccattc tctgttaagg ttttaaagaa
     1741 atgttctgta acctctgatt gttcaaaacc tttaattaaa ttctctggta tagaggctac
     1801 ctcataaacc aacggtacct tatcagcata acgtaccctt tccatacgaa ccacataatc
     1861 tgactgtgat aaatttaatt tttgaatttc ggtttcatta gctagttttc tttgaaatga
     1921 gattagttta ctggatggct tacgcccttg agaattgaca atttctgtaa aactagttgt
     1981 tccccgcatt ttttcttgaa cacgatgact agcaacatag gtgccactac caactcgtct
     2041 ctccaaaata ccttcttcaa ctaacaatgt aattgattgg cgaagtgtca tacgacttac
     2101 actataatca tccgctaaat ctctttcgct aggtaaccgc tgcccaatct tccaagtccc
     2161 tttatcgatt tcctttttaa tagcgtcgtg aattttaata tatgctggta acaatattct
     2221 ctcctcctag aatctacact tcattttacc atacttctat atatttctaa ctctaaaatc
     2281 gtgaaataat tcagaaaaat cataagcaat atttcttgat aagaaagcgt ttaatcaatt
     2341 attttaatga tgttaa
//
LOCUS       DATRQA010000195         2320 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.196, whole genome shotgun sequence.
ACCESSION   DATRQA010000195 DATRQA010000000
VERSION     DATRQA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.196"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(101..751)
                     /locus_tag="VBD27_001967"
     CDS             complement(101..751)
                     /locus_tag="VBD27_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001900118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO7025935.1"
                     /translation="MCKYKAIIFDMDGVLFDTELFYYKRRERFLKQHGITINHLPMNF
                     FIGGNMKQVWKSVLGDQYDTWDIDKLQQDYSRYKEDNPLPYKDLIFQDCKRVIEKLHH
                     KGYLLGLASSSTRHDIMLALESFNLDTYFKVILSGEEFSESKPNPAIYNRAAELLDIP
                     KQQILIVEDSEKGIAAGIAAGIDVWAIEDKYFGMNQSQANVLVSDLSQFFVKLDNY"
     gene            845..2197
                     /locus_tag="VBD27_001968"
     CDS             845..2197
                     /locus_tag="VBD27_001968"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEO7025936.1"
                     /translation="MKVVKFGGSSLASSQQLYKVLNIIKSDYTRRFIVVSAPGKRYEE
                     DLKMTDALIQYYQNYINGKDIVKDQTWIINRYQEIISDLSLGSTIAEEITRSIEQLAS
                     LPIENNQFLYDCFLAAGEDNNAKLVATFFNQNDIPARYVHPNEAGIIVTKEPCNARII
                     PGSYDKIENLCLYNEVLVIPGFFGVTEDNQICTFSRGGSDITGSLIAAGIKADLYENF
                     TDVDGIFAAHPGVVKNPHAIPELTYKEMRELAYAGFSVLHDEALLPAYRGRIPLVIKN
                     TNNPQQPGTKIVLKHTRSNIAVTGIASDSRFASINVSKYLMNREVGFGRKVLQILEDL
                     NISFEHMPTGIDDLSIVLREKELTPIKEQEILNYLTRKLEVDYVDIQHNLSTIVIVGE
                     NMKSQIGVTATATQALSREKINITMISQGSSEVSIMFVIDSKDEKRAIKALYETFFQK
                     "
BASE COUNT          815 a          408 c          355 g          742 t
ORIGIN      
        1 ataatttgat tatcaaatta tcatgctttg tattttatca taaacctagc tttcatggca
       61 agataaaaaa taaaaaaaaa cggaattaaa cctcccgttt ttaatagtta tctaatttca
      121 caaaaaattg actcagatcg ctcactaata cattagcctg cgactgattc attccaaagt
      181 atttgtcttc aatagcccac acatcaatac cagctgcaat gccagctgca atccccttct
      241 cactgtcttc tacaataaga atttgctgtt taggaatatc taaaagttct gctgctctat
      301 tataaatagc tggattaggt ttactttctg aaaattcctc accagacaga attaccttaa
      361 aatatgtatc cagattaaaa ctttctaatg ctaacatgat atcatgtcta gtagaacttg
      421 aagctagacc caaaagatag cctttgtgat gaagcttttc aatgactctt ttacaatcct
      481 gaaaaattaa atctttgtat ggcaagggat tatcctcttt atagcgacta taatcttgtt
      541 ggagcttatc aatatcccag gtatcatatt gatcacctaa aacagatttc caaacctgct
      601 tcatatttcc tccaataaaa aaattcattg gtaaatggtt aattgtaatt ccatgttgtt
      661 ttaaaaaacg ctctcgcctt ttataataaa ataattcagt gtcaaataga acaccatcca
      721 tatcaaatat tattgcttta tatttacaca tgtaactatt atatcattct tttaaattta
      781 gaggtaatga caaattttct gacttttgtt ataatttatc taaaattcat tcttgaggta
      841 acctatgaaa gtagtcaaat ttggaggaag ctctttagca tcttcccaac aattatacaa
      901 agtactaaat attatcaaat cagactacac tagacggttt attgtcgttt cagctccagg
      961 gaaacgctac gaagaagact taaaaatgac agatgctttg atacaatatt atcaaaatta
     1021 tattaacggt aaagatattg ttaaagacca aacttggatt atcaatcgtt atcaagaaat
     1081 tattagtgat ttgtctttag gaagcactat tgcagaagaa attactcgct ctatagagca
     1141 gctagcttca cttcccatag aaaataatca gtttttatat gattgttttt tagcagccgg
     1201 tgaagataac aacgcaaagt tagttgcaac gttttttaat caaaatgaca ttcctgcaag
     1261 atatgttcat ccaaacgaag caggaattat tgtaactaaa gaaccatgta atgcacgaat
     1321 tattccagga agttatgata agattgagaa cttatgtcta tacaatgagg ttcttgttat
     1381 ccctggattt tttggagtca cagaagataa ccaaatttgt accttttcaa gagggggatc
     1441 tgacattact ggatccctaa ttgcagcagg cataaaagca gacctttatg agaacttcac
     1501 agatgttgat ggtatatttg cagcgcatcc aggtgtagtt aagaaccctc acgctatccc
     1561 tgagcttact tataaagaaa tgcgtgaatt agcctatgcg ggtttttcgg ttttacatga
     1621 tgaagcttta cttcctgcct atcgtggcag aatccctctt gttattaaaa atacaaataa
     1681 tccccaacag cctggtacaa aaatagtttt aaagcatact cgtagtaaca tagcagtaac
     1741 tgggatcgct tctgatagcc gttttgctag cataaacgta tctaaatact taatgaatag
     1801 agaagtaggt ttcggccgaa aagtactaca aattttagag gatttaaata ttagttttga
     1861 acatatgcca actggcatag atgatctatc cattgtctta cgtgaaaaag aattgacacc
     1921 aatcaaagaa caagaaatct taaattacct aactcgtaaa ctagaagtag attacgttga
     1981 catccaacac aatttatcta caatcgtaat tgtaggtgaa aatatgaaaa gtcagattgg
     2041 agtcactgca acagcgacac aagccttatc aagagaaaaa atcaatatca ccatgatatc
     2101 acaaggttca agcgaagtct ccattatgtt cgttatagac agtaaggatg aaaaaagagc
     2161 tattaaagca ctatatgaaa cattcttcca aaaatagtac ctattacact acttacacta
     2221 ttagatagat aacaaatcgt cctaagtaag tttacttagg acgattttat ttagaacata
     2281 ggatagtttt ttcaatttta atcgtaacta cttggtatca
//
LOCUS       DATRQA010000196         2308 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.197, whole genome shotgun sequence.
ACCESSION   DATRQA010000196 DATRQA010000000
VERSION     DATRQA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.197"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..216)
                     /locus_tag="VBD27_001969"
     CDS             complement(<1..216)
                     /locus_tag="VBD27_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO7025937.1"
                     /translation="MKFLETNEYLRLIKICKEKSQHQSYAALYLIAKTGMRFAECLGL
                     TVNDIDYTNKYLSINKTWDYHFNQRYLP"
     gene            complement(219..485)
                     /locus_tag="VBD27_001970"
     CDS             complement(219..485)
                     /locus_tag="VBD27_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEO7025938.1"
                     /translation="MAYFRKRDNGWEYRISYKHIIGNILLCNITSTKYQQVINHYSQT
                     HVQDTVKRFNIHVKACVSMAVHEGYIEKNFCLFTKVKAKNKGRK"
     gene            485..889
                     /locus_tag="VBD27_001971"
     CDS             485..889
                     /locus_tag="VBD27_001971"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO7025939.1"
                     /translation="MIYSTLAKEQGVQGYLDGKGSLRDICKWYDISSRSVLQKWIKRY
                     TSGEDLKATSRGYSRMKQGRQATFEERVEIVNYTIAHGKDYQAAIEKFGVSYQQIYSW
                     VRKLEKNGSQGLVDRRVKGLESRPDLTEIEQL"
     gene            1051..1251
                     /locus_tag="VBD27_001972"
     CDS             1051..1251
                     /locus_tag="VBD27_001972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7025940.1"
                     /translation="MPIQALCQLLKGSRSGYYKWLNRQKTDFETKNTKLMAKIKELHR
                     LYNGILGYRRMTTFINRQLGTT"
     gene            1273..1533
                     /locus_tag="VBD27_001973"
     CDS             1273..1533
                     /locus_tag="VBD27_001973"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7025941.1"
                     /translation="MMNILGISSVIRRVSHACTKAGDRFYEENILNREFTATAHNQKW
                     CTDVTYLQYGLGAKAYLSAIKDLYNGSIIAYEISHNNEIHLL"
     gene            1659..1868
                     /locus_tag="VBD27_001974"
     CDS             1659..1868
                     /locus_tag="VBD27_001974"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7025942.1"
                     /translation="MSRIGKCIDNAPTESFFGFFKTESYHLKKYNSYEELVNDVARYI
                     EFYNTQRYQSKLNNLTPLEFRNQVA"
     gene            complement(2041..2202)
                     /locus_tag="VBD27_001975"
     CDS             complement(2041..2202)
                     /locus_tag="VBD27_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEO7025943.1"
                     /translation="MGGIGAHKFYAGKIGQVFLYIIFSITFIPGIIDLIEALMALGKQ
                     TDEYENIIV"
BASE COUNT          756 a          403 c          434 g          715 t
ORIGIN      
        1 tggcaggtat ctttggttga aatgataatc ccaagtttta ttaattgata aatatttgtt
       61 agtgtaatca atatcattaa ctgttaaacc taaacattca gcgaagcgca tgccagtttt
      121 agcgatgagg tataacgctg catacgattg atgttgtgat ttttctttac aaatttttat
      181 caagcgtaag tattcattgg tttcaagaaa ttttatcttt atttacgccc cttatttttt
      241 gctttaacct tagtgaataa acaaaaattt ttttctatat atccctcgtg aacagccatg
      301 gatacgcagg cttttacatg tatgttaaaa cgctttactg tatcttgcac atgcgtttga
      361 ctataatgat ttatgacttg ttgatattta gtggaagtaa tattgcaaag taatatattt
      421 cctattatat gtttatacga tattcgatat tcccacccgt tgtcgcgttt acggaaatac
      481 gccattgata tactccacat tagctaaaga acagggtgtt caaggctacc ttgatggaaa
      541 aggctctctt agagatattt gtaaatggta tgatatctca agtcgctctg ttctccaaaa
      601 gtggataaaa cggtatacta gtggtgaaga cttgaaagcc actagtagag gatatagccg
      661 tatgaaacaa ggaaggcaag ccacatttga agaacgtgta gagattgtta actacaccat
      721 tgcccatggg aaagactatc aagcagctat tgagaagttt ggtgtttcct accaacaaat
      781 ttattcttgg gtgcgtaagc ttgagaagaa tggctcacaa ggtttggttg atagacgtgt
      841 gaaagggttg gagagtaggc ctgatttaac cgagattgag caactttaac tcaagattaa
      901 acaattggag gaacgtaatc gtctcttaga aatcgaggtt agtttactaa aaaagttaga
      961 agacatcaaa cgaggaaaca gacggtaaga ctaggtaagc atttagcgga gttccaagta
     1021 atcaagaatt attacgatga ggaatctaat gtgcctattc aggccttatg ccaactcttg
     1081 aaggggtctc gttcaggcta ttacaagtgg ctcaatcgtc aaaaaacaga ttttgagaca
     1141 aaaaatacaa agctaatggc taaaatcaag gaacttcata gactctacaa tggtatctta
     1201 ggttatcgcc gtatgacaac atttattaat cgtcaacttg ggacaactta aaacaagaaa
     1261 cggattcgtt gattgatgaa cattctgggg attagttcag tcattcgtcg tgttagccat
     1321 gcttgtacaa aagctggtga cagattttac gaagaaaata ttcttaatcg tgaatttaca
     1381 gccacagctc ataaccagaa atggtgcaca gatgtcacct atcttcaata cggtctggga
     1441 gctaaagctt atctcagtgc gattaaagac ctgtataacg gttctattat cgcttatgag
     1501 attagtcaca acaatgaaat ccacttgtta tgaagaccat taaaaagggg ctagagctca
     1561 atccaggagc cacacctatc atccatagcg attgaggtag tcaatatact tccaaagaat
     1621 accgttatat catacaacaa gctggtctga ccttatccat gtcccggatt ggcaaatgta
     1681 ttgataatgc accaactgaa agtttctttg ggtttttcaa gactgagtct taccacctta
     1741 agaaatacaa ctcttatgag gagttggtca atgatgtggc acgttatatc gaattctaca
     1801 acacacaacg ttatcaatca aaattaaaca atctgactcc tctagaattc aggaatcagg
     1861 ttgcataact tatcttttat tatttgactg tctacttgac agggagccgt tcagattgct
     1921 taacctttct aaatttgcta aaatagctac aagaaaacga gccatttaat gcttatttct
     1981 tatactgtct tgcctcacgc tctcctcgac caaaaattga gcgtgaggct ttttgtttca
     2041 ttaaacgatg atattttcat attcatcagt ttgttttccg agagccatca aagcttcgat
     2101 aaggtcgata attccaggaa taaaggtaat actaaaaata atatataaaa aaacctggcc
     2161 tatttttcct gcgtaaaatt tatgcgctcc aatgccgccc aaaagaacgt taataaaaca
     2221 taaactacta tgttagcata agactttatt tttacaactg aatttcatat aaatggatta
     2281 gagtaaggga taaaagaaat tagcatag
//
LOCUS       DATRQA010000197         2298 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.198, whole genome shotgun sequence.
ACCESSION   DATRQA010000197 DATRQA010000000
VERSION     DATRQA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.198"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..406)
                     /locus_tag="VBD27_001976"
     CDS             complement(<1..406)
                     /locus_tag="VBD27_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HEO7025944.1"
                     /translation="MRRKIVLLFVVPMLIVLGILGVVVHYYGSALNIYLLPPSSERYG
                     RVILDRVEQRGLYSQGRQWQIIRQRSEKKLKTSKSYQESRNIVQEAVRYGGGKHSQIL
                     SKETVRRDTLDSRYPEYRRLNEDILLITIPSIS"
     gene            572..1060
                     /locus_tag="VBD27_001977"
     CDS             572..1060
                     /locus_tag="VBD27_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEO7025945.1"
                     /translation="MAKTSKKLTKSLGPIGKLISIIPDTTELIGKAIDNSRPIIEKEL
                     DRRHEKKQTYTQLDNVVHLDINDAQVYLEQRHFNVSRIVAEPNSKWATLHPNQVVNMS
                     PKAGRVRIGSLVKLYYITEDILEESKTLLLSKSEKSKARQQRLTEVLSTPSQLLKKNR
                     NK"
     gene            complement(1092..1940)
                     /locus_tag="VBD27_001978"
     CDS             complement(1092..1940)
                     /locus_tag="VBD27_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO7025946.1"
                     /translation="MKIKKLLGLTTTVVISALILGACGQSKNEDAKVVRVGTMVKSKT
                     EKARWDKIEELVKKKGVKLKFTEFTDYTQPNKALESDEIDINAFQHYNYLNNWNKANK
                     TNLVSVAETYFTSFRLYSGTKNGKGKYQTVSEIPNKATITIPNDAVNESRSLYLLQSA
                     GLLKLKVSGDTLATMSDVVSNPKSLDLKEVDAAQTARSLDSTDAAVINNDFVTEAGIN
                     PKSAIFIEPKSKNAKQWYNLLVAQKGWQDKSKAKAIKEVVKAYHTDAVKKVIEKTSQG
                     LDQPVW"
     gene            complement(2073..>2298)
                     /locus_tag="VBD27_001979"
     CDS             complement(2073..>2298)
                     /locus_tag="VBD27_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7025947.1"
                     /translation="DVTAWDYGEYVEIDDFYVQESYRGQGIGTRLQFDASRGFKKVIL
                     ISEEENRKMYEHQGYEEVAYYWTALRSPRK"
BASE COUNT          721 a          423 c          347 g          807 t
ORIGIN      
        1 tacttataga aggaatagta attaagagaa tgtcttcgtt taatcttcga tattctggat
       61 atcgactatc tagggtatct cttcgtacag tctctttgga taatatttga ctatgtttac
      121 cgcctccata ccgtactgct tcctgtacaa tgttgcgact ctcttgataa gattttgacg
      181 ttttcaactt tttttctgat cgttgtctta taatttgcca ttgacgacct tgcgaataca
      241 aaccacgctg ttcaacacga tctaaaataa cacgtccata acgttcgcta ctaggaggta
      301 atagataaat atttaatgct gaaccataat aatgaactac cactcctaaa attcctaaaa
      361 ctataagcat tggaactaca aaaagtaaaa ctatttttcg ccgcatctct tgctccccct
      421 ttctctttaa gtctattata tggcaaaatg gtttactata accttacatc ttaagaccta
      481 tttcttacaa atttgaaagt tttaaaagct ttataaacta taattttgat ataatatctt
      541 acataacaaa tggaaagtac ggtgaaaaca tatggccaag acaagtaaaa aattgacaaa
      601 atctttaggg cctattggta aattaatttc aataatccct gacacaactg aacttattgg
      661 aaaagcaatt gataattctc gtccaattat tgaaaaagaa cttgaccgtc gtcatgaaaa
      721 aaaacagact tatactcagc ttgataatgt agtacacctt gatatcaatg atgcgcaagt
      781 ttacttagag caacgccatt ttaatgtatc aaggattgtt gctgaaccaa attcaaagtg
      841 ggcaacccta catcctaatc aagttgtgaa tatgtcgcct aaagctggta gggtcagaat
      901 cggttcatta gtgaagttgt actatattac agaggatatc cttgaagaaa gtaagacact
      961 acttttatct aaatccgaaa aaagtaaagc acgacaacaa cggttaacag aagtattgag
     1021 tacccctagt caattactca aaaaaaatcg aaataaataa gcatgatcga attgaccatg
     1081 cttatttatt tttaccaaac aggttgatct aaaccttgag atgttttttc gataactttt
     1141 ttaaccgcat ctgtatggta agctttcaca acttctttaa tggctttagc ttttgatttg
     1201 tcttgccaac ctttttgagc aactaaaagg ttgtaccatt gttttgcatt tttacttttt
     1261 ggttcaataa aaatagctga tttaggattt attcctgctt ctgtcacaaa atcattatta
     1321 ataactgctg catctgtgga atccaaagaa cgtgcggtct gtgctgcatc tacttctttt
     1381 aaatctaaag atttaggatt ggaaacaaca tctgacattg ttgctaatgt atcacctgat
     1441 actttcaatt ttagcaagcc tgctgattgt aacaagtaga gagagcgact ttcgttaact
     1501 gcatcgtttg ggatagtaat agttgcttta tttggaattt cagaaactgt ttggtattta
     1561 cctttaccgt tcttagtacc agagtataat ctaaaggaag taaagtatgt ctcagcaacg
     1621 gaaacaagat tggtcttatt tgctttattc caattgttta agtaattata atgctggaaa
     1681 gcattaatat caatctcatc actttctaaa gctttattag gttgtgtata atctgtgaat
     1741 tccgtaaatt ttagcttaac gccttttttc ttaactaatt cttcgatttt atcccaacga
     1801 gctttttcag ttttactctt taccattgtt ccaactctaa cgactttggc gtcttcattt
     1861 ttagactgac cacaggctcc taaaataagt gctgaaatta ctactgttgt cgttaaccct
     1921 aatagctttt tgattttcat aatttatttc tcctttgata tggttgtatg attaatcaac
     1981 tgatactagt gtaacaaaaa gctaatggat taaaaaatac ttattatcaa tcaagagata
     2041 tagcttttaa ctatatcatt tcttttataa ttttactttc gagggctccg taaagcggtc
     2101 caataatagg cgacttcctc ataaccttgg tgctcataca tttttctgtt ttcttcttct
     2161 gaaattaaga taactttctt aaacccccta cttgcatcaa attgtaggcg cgtaccaatt
     2221 ccttgtcctc tgtaggactc ttggacataa aaatcatcta tttccacgta ttcaccatag
     2281 tcccaagctg ttacatct
//
LOCUS       DATRQA010000198         2287 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.199, whole genome shotgun sequence.
ACCESSION   DATRQA010000198 DATRQA010000000
VERSION     DATRQA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.199"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            92..1432
                     /locus_tag="VBD27_001980"
     CDS             92..1432
                     /locus_tag="VBD27_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO7025948.1"
                     /translation="MNQNHSVKRSLSEVNQSVEVPHNSSFWNTLRAFLGPGALVAVGY
                     MDPGNWITSVIGGATYRYLLLFVVLVSSLMAMQLQQMAGKLGIVTRQDLAQATASRLP
                     KPLRYLLFIIIELALIATDLAEVIGSAIALHLLFGWPLLLSIMITILDVFLLLLLMKL
                     GVQKIEAFVSVLILTILIIFTYLVVLSQPDLDAMFKGFLPHHELFNISHEGKNSPLTL
                     ALGIIGATVMPHNLYLHSSLSQTRRVDYHNKSSIKKAVRFMTLDSNIQLSLAFVVNSL
                     LLVLGASLFYGHANDISAFSQMYLALSDKTITGAVASSFLSTLFAVALLASGQNSTIT
                     GTLTGQIVMEGFLHFKLPQWLIRLCTRLLTLLPIFAIALLVGGEENTLDQLIVYSQVF
                     LSLALPFSIFPLIYFTSQKSIMGEHANTKWNTYLAYLIAIILTLLNLKLIMDLL"
     gene            complement(1534..>2287)
                     /locus_tag="VBD27_001981"
     CDS             complement(1534..>2287)
                     /locus_tag="VBD27_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025949.1"
                     /translation="ILTIIVNYLSATGLLTGNSQKSLSDRYQTLLTPAPLAFSIWSVI
                     YLLTFLVILRAIFSKSQSYQDNFASIFPYFLGLLLVNNIWTVFFTSNLIGLSTIIIFA
                     YCILLVIIIKILSKNKSKLLLRITFGIHAGWLLVASLVNLAVYLVKIDFNYPLPKVYI
                     AIIALIFITVLSLYLARVLQNAYLILSVFWAWLMVFKAHLEGNFQHLYFSFQILSLIA
                     AIILGVSFFIIGYYNVKERYKQKRTSPYSRVL"
BASE COUNT          725 a          411 c          361 g          790 t
ORIGIN      
        1 caatcgatta ttaagtagca ttaattttta attgtttttc tattttctat ttgttataat
       61 ggcttgagat tactagaaag tatagtatta catgaatcaa aatcattccg tcaaaagatc
      121 cttatccgaa gttaatcaga gcgttgaggt tcctcacaat agctcttttt ggaacacttt
      181 aagagctttt cttggacctg gagctttggt tgcagttggt tatatggatc caggtaactg
      241 gattactagc gttattggtg gagctaccta tcgttactta ctattgtttg ttgttttagt
      301 atcttctttg atggcgatgc aattacaaca aatggctggc aagcttggta ttgttacacg
      361 acaggactta gcacaagcaa cagcttctcg actcccaaaa ccgcttcgtt acctgctatt
      421 tattatcatt gagttagccc ttatcgctac tgatttagca gaagtcattg gctctgccat
      481 tgctcttcac ttattatttg gctggcccct gttactgtct atcatgatta ctattttgga
      541 tgtattctta cttttactct taatgaagtt aggcgtccaa aaaattgaag cctttgtttc
      601 tgttctcata ttgacaattt tgataatctt tacatattta gttgttttat cacaacctga
      661 tttagatgct atgttcaaag gatttttacc acaccatgag ctttttaaca tatcacatga
      721 agggaaaaat agtcctttaa ccctcgctct tggcattatc ggcgcaacag tcatgccaca
      781 taatctttat ttacattctt ctttatctca gactagacgc gtggattatc ataataaatc
      841 ttcaataaaa aaagctgtac gtttcatgac cttagattct aacattcagc tatcacttgc
      901 atttgtcgtg aattcgctac tattagtttt aggagctagt ctattttatg ggcatgctaa
      961 tgatatcagt gccttttctc aaatgtatct tgccttatct gataaaacta ttactggtgc
     1021 agtagccagt tctttccttt ctaccctttt tgcagttgcc ctactagcaa gtggtcaaaa
     1081 ttcaacaatt acaggtactt taacaggaca aatcgttatg gaaggtttcc ttcacttcaa
     1141 attacctcaa tggttaataa ggctttgtac tagattatta actcttcttc caatttttgc
     1201 tatcgcccta ctagttggag gcgaagaaaa tacgttagat caactaatcg tctattcaca
     1261 agtttttctt tccttagctt taccattttc aatctttcca ttgatttatt tcacatcgca
     1321 aaaatcaatt atgggagagc atgctaatac taaatggaat acctatttag cataccttat
     1381 cgctatcatt ttaacattac tcaatttgaa attaatcatg gatttacttt aatacaatac
     1441 aaaaaagccc cactaatgaa ctgaacctct atcatgagac atacatctaa tgatagagga
     1501 aagtacataa tgatggagct ttttgtattt cttttaaaga acccgtgagt aaggactagt
     1561 acgtttttgc ttgtacctct ccttgacatt ataataaccg atgatgaaaa acgatacgcc
     1621 aaggataatt gctgcaatca aagataaaat ttggaaacta aaatataggt gttgaaaatt
     1681 tccttctaaa tgtgctttaa aaaccatcaa ccaagcccaa aaaacggata agattaagta
     1741 agcattttgt aataccctag ccaggtaaag acttaaaaca gttataaaaa tcaaggcaat
     1801 gatagctata tatacctttg gtaatgggta attaaaatca attttaacta agtagaccgc
     1861 taaattgacc aatgacgcaa ctagcaacca tcctgcatga attccaaatg ttattctaag
     1921 cagtaatttt gatttatttt tacttagtat cttgataatg ataaccagaa gaatacagta
     1981 agcaaaaatt attattgttg ataggccaat caaattgctc gtaaaaaata cagtccatat
     2041 attattaact aatagaagac ccaaaaagta tggaaaaatg ctagcaaagt tatcttggta
     2101 agattgactc ttactgaata tagctcttaa tattactaaa aaggtcaata aatagatgac
     2161 tgaccaaatt gaaaaggcta atggtgctgg tgtcaagagc gtttgataac gatcagacag
     2221 actcttttga ctgttccctg ttaacaaacc tgttgcggat aaataattca ctattattgt
     2281 taagata
//
LOCUS       DATRQA010000199         2277 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.200, whole genome shotgun sequence.
ACCESSION   DATRQA010000199 DATRQA010000000
VERSION     DATRQA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.200"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            143..1138
                     /locus_tag="VBD27_001982"
     CDS             143..1138
                     /locus_tag="VBD27_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO7025950.1"
                     /translation="MRTYITNLNGHSITSTAQIAQNMVTDIAVSLGFRELGIHSYPID
                     TDSPEEMSKRLDGICSGLRKNDIVIFQTPTWNTTTFDEKLFHKLKIFGVKIVIFIHDV
                     VPLMFDGNFYLMDRTIAYYNEADVLIAPSQAMVDKLQSYGLTVKKILVQGMWDHPTNI
                     TLQAVNHKKLVHFPGNPERFNFIKNWRIPTELHVYTDHNMQLPTTVVKEPYQSDEQLI
                     MKMSEGGYGLVWMDDRDKQYQSLYCPYKLGAYIAAGIPVIIQKGIANQDIIEKNNLGF
                     IIEKIDDISNIVESTTEEEYMEIVSDVRRFNPLVRQGYFTRKLLTDAVFSALNSM"
     gene            1146..>2277
                     /locus_tag="VBD27_001983"
     CDS             1146..>2277
                     /locus_tag="VBD27_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEO7025951.1"
                     /translation="MKELEEIVVFPINKSLDYILENHSSVIRFGDGEFDIIMGKSIPY
                     QDYNPELADRLLQLLHKQSTNDLLVCLPDMFQNRQRYNQSAQLFWKNHFQKFGNFYRQ
                     HCYQDWYGSSFISRPYMDLQDKESAVESFKKLKLLWDKRDILVVEGETSRSGVGNDLF
                     NNCNSLKRIICPSKNAYIKYDEILEAVERYAQGRIILLMLGPTAKILVRDLSDRGFQA
                     IDIGHIVSEYEWYKMGATQKIKFSHKHTAEFNDDENINLIDSQNYIDEIVKTITIDDT
                     EFRDTELISIILPVYNMSGSLSRCLDSILRQVYSTFEVILVNHGSTDGSGNICEKYAM
                     KDERIRLFHEEHQVITSVWNFALEKSKGKYVTFVNPDDFLDES"
BASE COUNT          776 a          307 c          439 g          755 t
ORIGIN      
        1 aacatgacaa gcgacctact ttttatttgg tagtacttat acttgttact gtagcagtta
       61 tattgtcgtt atttaaatat tttttataga ttcctattaa tggtagaaaa gaagcctgtt
      121 gttttttaga taggggaagt gtgtgaggac atatattaca aacttgaatg gacattcaat
      181 cactagtaca gcacaaatag ctcaaaacat ggtaacagat atagcagtaa gcttaggttt
      241 tcgtgagctg ggaatacatt cttatccgat tgatactgat tctcctgagg aaatgagtaa
      301 gcgtttagat ggaatctgtt ccggacttag aaaaaatgat attgtcatat ttcagacacc
      361 tacatggaac actacaactt ttgatgaaaa attatttcac aaattaaaaa tatttggtgt
      421 aaagattgtt atttttatac atgatgttgt accgctaatg tttgatggaa atttttattt
      481 gatggataga actatagctt attataatga agcagatgtt ttaatagccc ctagtcaagc
      541 aatggtcgat aagcttcaaa gttatggctt gacagtaaaa aaaatcttag ttcaaggaat
      601 gtgggatcat cccaccaata ttactttaca agcagttaat cataagaagc ttgttcattt
      661 tcctgggaac ccagagcggt ttaacttcat aaagaattgg agaattccta ccgagttaca
      721 tgtttatact gaccataaca tgcaattacc caccactgtg gttaaagagc catatcaatc
      781 ggacgaacaa ttaattatga aaatgagcga agggggatat ggcttagtct ggatggatga
      841 ccgtgataaa caatatcaat ctctttattg cccttacaaa ctgggcgctt atatagcagc
      901 aggaattcca gtgattattc aaaaaggtat tgctaatcaa gatataatag aaaaaaataa
      961 tttgggattt attattgaaa aaattgatga tattagtaat atagttgaaa gtactactga
     1021 agaggaatat atggaaattg ttagcgatgt caggcggttt aatcctttag ttagacaagg
     1081 atattttacg cgaaaactat taaccgatgc agtatttagt gcattaaata gcatgtgagg
     1141 ggaatatgaa agaactagaa gaaatcgtag ttttccctat aaacaaaagt ttggattaca
     1201 ttttggaaaa tcattcgtca gttattcgct ttggggacgg tgaatttgac attataatgg
     1261 gaaaatcgat tccttatcaa gattacaacc cagagttagc agataggtta ttgcaacttc
     1321 ttcataagca aagtacgaat gatctattag tatgtcttcc agatatgttt caaaatcgac
     1381 aaagatataa tcaatcggca caactttttt ggaaaaatca ttttcaaaaa tttggcaatt
     1441 tctataggca acattgttat caagattggt atggttcttc ttttatttca aggccatata
     1501 tggacttaca agataaggag tctgctgtag aatcatttaa aaaattaaag ttactttggg
     1561 acaaacgcga tattttggta gttgaagggg aaacttcgcg gtcgggtgta ggtaatgatc
     1621 tttttaataa ttgcaatagt ttaaaaagga ttatttgtcc ttctaaaaat gcttatataa
     1681 aatatgatga aattcttgag gctgttgaaa gatatgctca agggaggata atcttattga
     1741 tgctaggtcc gactgcaaaa attttagtaa gagatttatc tgatagagga tttcaagcca
     1801 ttgatatcgg tcatattgtt tctgaatatg aatggtataa gatgggagct actcaaaaaa
     1861 taaaattttc tcataaacac accgctgagt ttaacgatga tgaaaatatt aatttaattg
     1921 acagtcagaa ttatattgat gagattgtta aaacaataac tatcgatgat actgaattca
     1981 gggatactga attaatatca atcatacttc ctgtctataa tatgtctggt tctttatcaa
     2041 ggtgtttaga ttctatcctt aggcaagttt actcaacttt tgaggtgatc ttagtcaacc
     2101 atggttctac agatggaagt ggtaatattt gcgagaaata tgcgatgaag gatgagcgta
     2161 ttcgtttatt tcatgaagaa catcaagtta ttacatctgt atggaacttc gcattagaga
     2221 aatctaaagg gaagtatgtt acttttgtga atccagatga ttttcttgat gagtctt
//
LOCUS       DATRQA010000200         2259 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.201, whole genome shotgun sequence.
ACCESSION   DATRQA010000200 DATRQA010000000
VERSION     DATRQA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.201"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            21..680
                     /locus_tag="VBD27_001984"
     CDS             21..680
                     /locus_tag="VBD27_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEO7025952.1"
                     /translation="MSLEDYYLLSKKFSQVMTLDSHSSDDGYTIRSLYFDSLEDVDWQ
                     EKEDGVEIRRKIRLRNYGSQSNFAKLEMKQKQGANQKKRSLKLSKTDAQALINGNNSV
                     LLNYPDTFAAECYSRITTQLYQPKTVIEYQRKAFVAKENNIRITFDHHIIGTEASYDI
                     FSEDLNQNYLLDPYLVILEVKYNGFLLSYIKELLDSVDRSELSASKYCLGRAVSKHYR
                     F"
     gene            731..1408
                     /locus_tag="VBD27_001985"
     CDS             731..1408
                     /locus_tag="VBD27_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEO7025953.1"
                     /translation="MKDLLRNSLEQSGNLSFQDMILHILVAALLSVVIYVSYAYTHSG
                     TAYSKKFNVSLMTLTVLTATVMTVIGNNVALSLGMVGALSVVRFRTAIKDSRDTVYIF
                     WTIVVGICCGVGDYVVAALGSSVIFILLWVMGRVKNENRMLLIVKCDRTLEVDLEGIF
                     FQYFDGKAVQRVKNSTTNTIEMIFEISRKDYDKQLHVDNQLTEKVYQLGNIDYFNIVS
                     QSDEING"
     gene            1401..>2259
                     /locus_tag="VBD27_001986"
     CDS             1401..>2259
                     /locus_tag="VBD27_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEO7025954.1"
                     /translation="MGRLMRGKVIYGTTLIGLFLFLFFYFWIPKHHIERIHHHRIKQV
                     DAKSDLTGFKTHLPIISIDTKQQVIPLVTKEGGKYVKARDNINVDIELRDSPSRSHHL
                     SEKPRIRTKGLISYRGNSSRYFDKKSLKVKFVTNKLKEKKHRLAGMPKESEWVLHGPF
                     LDRTLLRNYLSYNIAGEIMSYAPNVRYCELFVNGEYQGVYLAVENIEQGEQRVPIEKS
                     DKKLHKTPYIVAWDREHKAKQKLDNYVHYTHQSGISALDVKYPGKQRLTSKQLEFINK
                     DINHIEKVLY"
BASE COUNT          783 a          312 c          443 g          721 t
ORIGIN      
        1 agacaggaaa aaaatattta atatcacttg aagattacta tcttttatct aagaaatttt
       61 cccaagtaat gacgctcgat tcgcatagtt cagacgacgg ctatactatc cgttcgctat
      121 actttgattc tttagaagat gttgattggc aagaaaagga agatggtgtt gagatacgaa
      181 gaaagattcg tttgcgcaat tatggtagtc aaagcaattt tgcgaaatta gaaatgaaac
      241 aaaaacaagg tgctaatcaa aaaaagcgct ctctaaaatt aagcaagaca gatgctcaag
      301 cgctgattaa tggtaacaat agtgttttat taaattatcc ggatacattt gctgcggagt
      361 gttattcaag gataacaacg caactttatc aacctaaaac ggttattgag taccaaagaa
      421 aagcttttgt tgctaaggaa aacaatatcc gaatcacctt tgaccaccat ataataggta
      481 cagaggctag ttatgatatt ttctcagagg atttaaatca aaattattta ttagatcctt
      541 atcttgttat tttagaagtt aaatataatg gatttttatt gtcttatatt aaagaactct
      601 tagattcggt agatagaagt gaactttcag cgagtaagta ttgtttagga agagctgtta
      661 gtaaacatta tcgtttttag tgaaaaagta tatacgttaa ttattattaa atgtgagagg
      721 ataacaaaaa atgaaagacc tattacgaaa tagtctagag caaagtggaa atttaagttt
      781 tcaagatatg attttacata ttcttgtagc agctttattg agtgtagtta tttatgtttc
      841 ctatgcttat acgcatagtg gaactgccta tagtaaaaag tttaatgttt cattaatgac
      901 attgacggtc ttgactgcaa cagtaatgac cgttattggt aataatgtag ccttgtcatt
      961 gggtatggtc ggtgccttgt cagttgttcg ttttaggaca gccataaaag attcaagaga
     1021 tacagtttat attttttgga ccatagttgt tggtatctgt tgtggtgtcg gtgactatgt
     1081 ggtagctgca ttaggaagta gcgttatctt tatcttatta tgggttatgg gacgtgttaa
     1141 aaacgagaat cgtatgttat tgattgtgaa gtgcgataga acactagaag ttgatttaga
     1201 aggaattttc ttccaatatt ttgacggaaa agctgttcag cgtgttaaaa attcaacaac
     1261 taatactatt gaaatgattt tcgaaatctc tagaaaagat tacgataagc aactccatgt
     1321 agataatcag ttaactgaaa aagtgtacca attgggaaat attgattatt tcaacattgt
     1381 tagccaaagc gacgaaatca atgggtagac ttatgagagg gaaggttatt tacggcacaa
     1441 cccttatagg tctttttcta ttcttatttt tctatttttg gattcctaag catcacatcg
     1501 agagaataca tcatcatcgt ataaagcagg tagatgcgaa gagtgattta acaggattta
     1561 aaacccattt gcccattatc agcattgata caaagcaaca agttattcct cttgttacaa
     1621 aagaaggcgg aaaatatgtc aaagctaggg ataatattaa tgttgatatc gaattacggg
     1681 attctccaag tagatcacat catttatcag aaaagccgag aattaggaca aaagggttaa
     1741 tatcatatag aggaaattcc tctcgttact ttgataagaa gtcattgaaa gttaagtttg
     1801 ttactaataa gttaaaggaa aagaagcatc gattagcagg aatgcctaaa gaatcggagt
     1861 gggtattgca tggtcccttt ctagacagaa cattattaag aaattatctg agttataata
     1921 ttgctggtga gattatgtcc tatgccccaa acgttcgcta ctgtgagtta tttgtcaatg
     1981 gtgagtatca gggagtatat ctagcagttg agaatatcga acaaggtgag caacgagttc
     2041 ccattgaaaa aagtgataaa aagttgcata aaacacctta tatcgttgct tgggatcgtg
     2101 aacataaggc aaagcaaaag ttagataact atgtacatta tacacaccag tctggtatct
     2161 cagctcttga tgtgaaatat ccaggtaaac aaagattgac atcaaaacaa ttagagttta
     2221 ttaataagga catcaatcat attgaaaaag tcctttatt
//
LOCUS       DATRQA010000201         2253 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.202, whole genome shotgun sequence.
ACCESSION   DATRQA010000201 DATRQA010000000
VERSION     DATRQA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.202"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1002)
                     /locus_tag="VBD27_001987"
     CDS             complement(<1..1002)
                     /locus_tag="VBD27_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEO7025955.1"
                     /translation="MFLLLCILSMWLMIFGVAFYYFGSLHQSLTSRIIYQFVLTVLLT
                     TTAWFMGAYFLAFEGHFKTVFQFQEADGKQIVNCLFQLCFALYAVVMLIGSIIDRVQT
                     KRLLLAVVSWLFLVYTPLAYLIWNSEGVFAKMGVLDFSGGMVVHLSAGLSSYILAHVI
                     GKSEHQHNKVKNDSLFLGMILITFGWFGFNMGPVGEWNSQAIMILLNTIFAIIGGGLA
                     WTLAAKWNGEEEKTGSLLNGIIVGLVTSTAGVGYLLTWQLLAVTFFASLFTYFVTDYV
                     AKAFAIDDVVSSFGMNGIGGLLGSLGVGLFKLSHMPVQLLALATTILLSIIMTYIISK
                     "
     gene            1106..2233
                     /locus_tag="VBD27_001988"
     CDS             1106..2233
                     /locus_tag="VBD27_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEO7025956.1"
                     /translation="MKHYQTIIIGAGAAGIGFGSAMQRLGLTNFLIIEKGHIGESFLR
                     WPRTTQFITPSFTTNGFGFPDLNAVIPDTSPAFSFEKEHLSGVEYARYLQLVAAHYNL
                     PIQNETSVLSIDKRDSLFVIKTSKGDFSADYLIMATGEFQNPNTIDIKGADLGMHYGQ
                     VDNFHIKSDNPFIIIGGNESACDALTHLVYLGNQVELYTDTFGRKESNPDPSISLSPL
                     TKERLKHIQDHKKEYYSISEGKKAIEIKQIGKQYQVTFDDGSTAESFHKPILSTGFLN
                     TCHLIDGIALFEYDKNQLPIVTEDDESTIVNNCFLIGPSLRQRDTIFCYIYKFRQRFV
                     PVINHIAQREGIILDEDTLEFFKENQMYLDNLACCDVNCDC"
BASE COUNT          797 a          449 c          345 g          662 t
ORIGIN      
        1 tttagatatg atataggtca ttataattga caaaagaatt gtggtagcta atgctaacag
       61 ttgaactggc atatgtgata atttaaagag accaactcct aaacttccca acaatccccc
      121 aataccattc attccaaagg aggaaacgac atcatcaatg gcaaaagctt tcgcaacgta
      181 atccgttaca aagtaggtga agagggatgc aaagaatgtt acagctaata attgccaagt
      241 taagagatac ccaactcctg ctgtactcgt cacaagacca acaataatgc cattcaataa
      301 gcttcctgtt ttctcttcct caccattcca cttagcagcc aatgtccacg ccaaaccacc
      361 acctataata gcaaatatag tatttaaaag tatcataatg gcttgtgaat tccactcacc
      421 aactggaccc atattaaatc caaaccaacc aaatgtaata agtatcattc ctaaaaagag
      481 actatcattt ttaaccttat tgtgctgatg ttcactcttg ccaataacat gagctaaaat
      541 atacgaagaa agccctgcag ataaatgaac gaccattccg cctgaaaaat caagtactcc
      601 catctttgca aaaacaccct ctgaattcca aattaaataa gctaagggcg tatacaccaa
      661 gaaaagccaa gaaacaacag ctaataaaag tcgcttcgtc tgaactctat caataattga
      721 cccaataagc attactactg cgtaaagggc gaaacaaagt tgaaataaac aatttacaat
      781 ttgtttacca tctgcttctt ggaattgaaa gacagtttta aaatgacctt caaatgctaa
      841 aaaataggca cccatgaacc aagctgttgt cgttagcaaa acagtaagta caaactgata
      901 aataatcctt gaagtcaatg attgatgtag cgacccaaag taatagaatg caaccccaaa
      961 aatcatgagc cacatcgata aaatacataa tagtaaaaac acaaatagtc ctttctttac
     1021 aaacactctt ttatttgtta aactgtattg ttgttaactg gttaagtatt ttatcttaca
     1081 catttatttt tgtcaaggag aagacatgaa acattatcaa accataatta tcggtgcagg
     1141 cgctgcagga atcggttttg gttctgccat gcaacgatta ggacttacaa attttcttat
     1201 cattgaaaaa gggcacattg gagaatcttt tttaagatgg cccagaacaa cacaatttat
     1261 cacaccttcc ttcactacca atggctttgg ttttccagat ctaaacgctg ttattccaga
     1321 tacttctcct gctttctcat ttgaaaaaga acatctttct ggagtggaat atgcacgcta
     1381 cttacaatta gttgctgctc attataatct ccctatccaa aacgaaacat ctgttctatc
     1441 tattgataaa cgagatagcc tttttgttat taaaacttca aagggagatt tttctgctga
     1501 ttatcttatt atggcaacag gagaatttca aaatccaaat accattgata ttaaaggtgc
     1561 agatctagga atgcactatg gtcaagttga taatttccat atcaaatctg acaatccatt
     1621 tatcatcatt ggtggaaacg aaagtgcttg tgatgcacta acacatctag tatatttggg
     1681 aaatcaagtc gaattatata cagatacgtt tggtcgtaaa gagagcaatc ccgatccaag
     1741 catctcttta tcccccctaa ctaaagaacg tttaaaacat atacaagacc acaaaaaaga
     1801 atattatagt atctctgaag gcaagaaagc cattgagatt aaacaaattg gaaaacaata
     1861 tcaagttact tttgatgacg gtagtactgc ggagtctttc cataaaccta ttctttccac
     1921 aggttttcta aatacctgtc atctcattga cggaatcgct ttatttgaat acgataaaaa
     1981 tcagttgcca atagttactg aagatgacga atctactatc gttaataact gtttcttaat
     2041 tggtccaagc ttacgacaaa gagatactat tttctgctat atttataaat tcagacagcg
     2101 ctttgtccct gttattaatc atatcgcaca acgagaaggg attatattag acgaagatac
     2161 ccttgaattc tttaaagaaa atcaaatgta ccttgataac ctagcttgtt gcgatgtcaa
     2221 ttgtgattgc taaaaaatcg ctagctttat tgc
//
LOCUS       DATRQA010000202         2238 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.203, whole genome shotgun sequence.
ACCESSION   DATRQA010000202 DATRQA010000000
VERSION     DATRQA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.203"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..709)
                     /locus_tag="VBD27_001989"
     CDS             complement(<1..709)
                     /locus_tag="VBD27_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7025957.1"
                     /translation="MLKRERLQKIIEKVNINGIVTVNEIMEELDVSDMTVRRDLDELD
                     KAGLLIRIHGGAQKVNAAPIPQNYEKSNTEKYDIQTNEKLEIAQFAKQFINDGETIFI
                     GPGTTLEKLATQLLDFKIRVVTNSLPVFNILNQSSTLDLILVGGEYREITGAFVGSVT
                     INSIKSLNFSKAFVSSNGVFEKSIATYDEGEGEIQRIALNNSFEKFLLVDSQKFGKYD
                     FYTFYQLDDIDFVLTDHN"
     gene            919..1392
                     /locus_tag="VBD27_001990"
     CDS             919..1392
                     /locus_tag="VBD27_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7025958.1"
                     /translation="MFAVTQDILFIDAHSQEELFDLVSKALIKQHYVSPDYRQAVKER
                     EREFPTGLKIDLKDGTPIQYVAIPHTETQYCLVDRIFYVKNSQPITFKHMINPEEECR
                     VQDFFFIINSRNSNQSDILSNLITFFITKGNLDRLHELGDNKEKINHYLIEKGVF"
     gene            1394..1699
                     /locus_tag="VBD27_001991"
     CDS             1394..1699
                     /locus_tag="VBD27_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7025959.1"
                     /translation="MIKILAACGAGINSSHQIKDAIETQLGDRGYNVHCDAVMVKDIT
                     EEMVNKYDIFTPIAKTDLGFNVPIPVVEAGPILYRIPVMSEPVFTALEQVIKEHNLS"
     gene            1739..2226
                     /locus_tag="VBD27_001992"
                     /pseudo
     CDS             1739..2226
                     /locus_tag="VBD27_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
BASE COUNT          762 a          335 c          371 g          770 t
ORIGIN      
        1 tattatgatc tgttaataca aaatcaatat cgtctaattg ataaaaagta taaaaatcat
       61 atttaccaaa tttttggcta tcaactaaaa ggaatttttc aaaggaatta ttaagagcaa
      121 tgcgctggat ttcaccttct ccctcgtcat aagtagcgat agacttttca aaaacaccat
      181 tactactaac aaaagctttt gaaaaattaa gtgatttaat agagttaata gtaacagaac
      241 cgacaaaagc accagtaatt tctctgtact ctcctcctac aaggataagg tcaagtgttg
      301 aactttgatt cagaatattg aaaacaggaa ggctattggt tactacccta attttaaaat
      361 caagtaattg cgtagctaat ttctctaagg ttgttccagg cccaataaag attgtttcac
      421 catcattgat gaattgtttt gcaaattggg caatttctaa tttttcattt gtttgaatat
      481 cgtatttttc agtgtttgat ttttcataat tttgtggaat tggtgctgcg tttacttttt
      541 gtgcaccacc atggatgcgt attaacagtc ctgctttatc taattcatcc aagtcacgcc
      601 taacagtcat gtctgataca tcaagttctt ccatgatttc attaacagtg acaataccat
      661 ttatattgac tttttctatt attttttgta atctttctcg ctttaacata ataatctctc
      721 ctacctcttc attgtatata tcataccata aatagagcta aaataataat aattttgaaa
      781 aaaacaaaca ataataaaca aaaataaata aaaaacgtct tgtgatgttt tattttgttt
      841 tgtataatat tgatgtagat aagttgataa ggcttgtttg ccttacaaca aaatgtaacc
      901 gcttaactaa aaagaaaggt gtttgctgtg actcaagata ttttatttat tgatgcccat
      961 tctcaggaag aattatttga cttagtatca aaagcattga taaaacaaca ctatgttagt
     1021 cctgactacc gacaggcagt aaaagaaagg gagagagagt ttccaacggg gttaaaaatt
     1081 gatttaaaag atgggactcc tattcaatat gttgctattc cacacactga aacgcagtat
     1141 tgtctagtag atagaatctt ttatgtcaaa aatagtcagc caattacttt caaacatatg
     1201 attaatccag aagaggagtg tagggttcag gattttttct tcatcattaa tagtaggaat
     1261 agtaaccaaa gtgatatttt atctaatttg attaccttct ttattacaaa aggaaaccta
     1321 gatcggctac atgagcttgg cgacaataag gaaaaaatta atcattattt aatagaaaaa
     1381 ggagtgtttt aacatgatca aaatattagc agcttgtggt gcaggcataa attcaagtca
     1441 tcaaattaaa gatgctatcg agacacaatt aggagataga ggttacaatg tacattgtga
     1501 tgctgttatg gttaaggata tcactgagga aatggtcaac aaatatgata tctttactcc
     1561 tattgctaaa actgatttag gttttaatgt accaattcca gttgtagaag caggacctat
     1621 tctttatcgc ataccagtta tgagcgaacc ggtctttact gctttagaac aagttatcaa
     1681 agaacacaac ctatcataac atatttaaaa ttggaattag tcttaaagga gaaggaacat
     1741 gcaggtattt ttaaatattg tcaataaatt ctttgatcca gttattcata tgggttcggg
     1801 agttgtgatg ctaattgtca tgacaggttt agccatgata tttggagtga agttttctaa
     1861 agcacttgaa ggtggtatta agttagctat tgctcttacg ggtattggtg ctattattgg
     1921 tattttaact ggtgcttttt ccgaatcact tcaagctttt gttaaaaata caggaatcaa
     1981 tctaagcatt attgacgttg gttgggctcc attagcaact attacatggg gatcaccata
     2041 tacgctttac ttcttattaa tcatgcttat tgtcaatatt gttatgattg ttatgaaaaa
     2101 aaaacggata ccttagatgt tgatattttt gatatttggc atttatctat cacaggtctt
     2161 ttaattatgt ggtatgctaa acaaatgaaa cgccgtaatg cagaatatgc agctaatcaa
     2221 aaataaatag aataaaca
//
LOCUS       DATRQA010000203         2221 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.204, whole genome shotgun sequence.
ACCESSION   DATRQA010000203 DATRQA010000000
VERSION     DATRQA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.204"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..178)
                     /locus_tag="VBD27_001993"
     CDS             complement(<1..178)
                     /locus_tag="VBD27_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7025960.1"
                     /translation="MNQEKKRLNLIGLILLAQAIVLSLATTLFAEILQNDVWIGIAST
                     LIALLIPCFFMGFKN"
     gene            complement(483..1043)
                     /locus_tag="VBD27_001994"
     CDS             complement(483..1043)
                     /locus_tag="VBD27_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO7025961.1"
                     /translation="MRITIFCGASTGENPVYSEKTVALAQWMAQNKHALVYGGGKVGL
                     MGVMADTVIANGGYTTGVIPTFLRDREIAHENLSELIIVNNMPERKAKMMLLGDAFIA
                     LPGGPGTLEEISEVISWSRIGQNDNPCILYNVNGYFNDLKNMFDHMVSEGFLSLEDRE
                     NVLFSDDIAEIEDFITNYKVPSTRQY"
     gene            complement(1040..1357)
                     /locus_tag="VBD27_001995"
     CDS             complement(1040..1357)
                     /locus_tag="VBD27_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO7025962.1"
                     /translation="MDITDYQKWVSEFYKKRNWYQYNSFIRSNFLSEEVGELAQAIRK
                     YEIGRDRPDETEQTDLENLNDIKEELGDVLDNIFILADQYNISLEEIISAHRTKLEKR
                     FET"
     gene            1571..2185
                     /locus_tag="VBD27_001996"
     CDS             1571..2185
                     /locus_tag="VBD27_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7025963.1"
                     /translation="MNKKSFFQCVILLVSFLVLTLAVKSQSDMISYLDNITSAFFQSI
                     RNSDLTNLMTIISTVVSPLTTSLIALVILGYQYFLNQRIAVWLFMLFFGTNALALLLK
                     DIIARHRPMNQLIFDSGYSFPSGHTISTFLLMILVLVVARQRLRRVLSQVVFVIFALV
                     ILASVIFSRLYLENHFLTDILGSLLLGASSYCGLSAIVSLKELQ"
BASE COUNT          703 a          369 c          359 g          790 t
ORIGIN      
        1 aatttttaaa acccataaaa aaacaaggaa tgagtaaggc gattaaagta cttgctatac
       61 ctatccagac atcattttgt aagatttcag caaatagtgt agtcgccaaa cttaaaacaa
      121 tagcctgagc taaaagtatc agcccgatta ggttaagtct ctttttttct tgattcaatt
      181 gattacctcc ttattaataa cggtaacgac aaagaaatat tataatgtat aatgtatagg
      241 tttctaagtc attagtcaat agggacgagc acttggatac ttctaataag ataaaataca
      301 tagacaagac tatggttttg gcttctctaa agattaccta aaaatttata agttctcgag
      361 cagaaaggat gagtttggat attgagagga tgggcttgac cagctgaaag aaaaatgaac
      421 caagggtaat cgcttcgaaa aaagaaggaa atttcaatta gaaacttcct ttttttatca
      481 atctagtatt ggcgcgtgct agggacttta taattcgtta taaaatcttc gatttcagca
      541 atatcatctg agaataggac attttctcta tcttccagac taagaaaacc ctcactaacc
      601 atatgatcaa acatattttt taaatcatta aagtagccat taacattata taaaatgcaa
      661 ggattatcat tttgaccgat gcgagaccaa gagataactt cggaaatttc ttcaagggta
      721 ccaggtccac caggtagagc gataaaggca tctccaagta gcatcatttt tgccttacgt
      781 tcaggcatat tatttactat gataagctct gataaatttt catgtgcaat ttcacgatct
      841 cttaaaaatg tcggaataac accagtagta tagccaccat ttgcaatcac agtatcagcc
      901 atgactccca ttaagccaac ttttcctcca ccatacacca gagcatgttt gttttgtgcc
      961 atccattgtg ccaaggcaac tgttttttca ctataaacag gattttcccc agtacttgct
     1021 ccacaaaaaa tagttattct catgtttcaa acctcttttc taatttagtg cgatgtgcgc
     1081 tgataatctc ttctagtgag atattatact gatcagcaag tatgaagata ttatctaaga
     1141 catctcctag ttcttctttt atatcattta agttttccaa atcagtttgt tcggtttcgt
     1201 caggtctgtc acgtccaatt tcatatttgc gaatggcttg agccaattcg ccgacttctt
     1261 ctgaaagaaa gttacttcta ataaaagaat tgtattgata ccagtttctt tttttataga
     1321 attcgctcac ccatttttga taatctgtaa tatccattgt tccctccgtt tcaagtatat
     1381 tgtagcaata ttgctttatt aatgaaaatt tgaacgctaa ttaataggaa aagttcgtat
     1441 ttggtatttc ttctagtttg tgaatagtca tatttaaact taagaggtta gcgcgtgctt
     1501 taagcatttt ttaaatattt taaaccataa tgtatgaaga tttatctctt tattaaagtg
     1561 aggttatgta gtgaacaaga aatccttttt ccagtgtgtt atactacttg tttctttttt
     1621 agtgttaaca ttagcagtta aatctcaaag tgatatgata agttatttgg ataatatcac
     1681 ttcagctttt tttcaaagca tacgaaactc cgatttaact aacttaatga cgataatatc
     1741 aacagttgtc agccctttga caacgtcatt aatagcactt gttatccttg ggtatcaata
     1801 ttttttaaac caaaggatag ctgtgtggct atttatgtta ttttttggta ccaatgcttt
     1861 ggcattgctt ttaaaagata tcatcgcacg tcatagacct atgaatcaac ttatttttga
     1921 ctcaggttat agttttccca gtggacatac gatatcaacg tttttattaa tgattcttgt
     1981 tttagtggtt gcaagacaga gattgagaag agtattgtca caagtagttt tcgtaatatt
     2041 tgcattagta atccttgcat cagttatttt ttcaagactt tatttagaaa atcatttttt
     2101 aacggatatt ttagggtcgt tgttactagg agcatcgtca tattgtggtt tgtctgctat
     2161 tgtatctttg aaagagttac aataaaaata gtagttacca atgaattata aaagagcctt
     2221 t
//
LOCUS       DATRQA010000204         2185 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.205, whole genome shotgun sequence.
ACCESSION   DATRQA010000204 DATRQA010000000
VERSION     DATRQA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.205"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(71..1567)
                     /gene="malQ"
                     /locus_tag="VBD27_001997"
     CDS             complement(71..1567)
                     /gene="malQ"
                     /locus_tag="VBD27_001997"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEO7025964.1"
                     /translation="MKKRASGVLMHITSLPGDLGIGTFGREAYAFVDFLVETDQKFWQ
                     ILPLTTTSFGDSPYQSFSAVAGNTHLIDFDLLTLEGFISKDDYQNISFGQDPEVVDYA
                     GLFEKRRPVLEKAVKNFLQEERATRMLSDFLQEEKWVTDFAEFMAIKEHFGNKALQEW
                     DDKAIIRREEEALAGYRQKLSEVIKYHEVTQYFFYKQWFELKEYANDKGIQIIGDMPI
                     YVSADSVEVWTMPELFKLDCDKQPLAIAGVPADDFSDDGQLWGNPIYNWDYHKESDFD
                     WWIYRIQSGVKMYDYLRIDHFKGFSDYWEIRGDYQTANDGSWQPAPGPELFATIKEKL
                     GDLPIIAENLGYIDERAERLLAGTGFPGMKIMEFGFYDTTGNSIDIPHNYTENTIAYA
                     GTHDNEVINGWFENLTVEQKAYAENYMRRLPNEPITETVLRTLYATVSQTTITCMQDL
                     LDKPADSRMNMPNTVGGNWQWRMRKEDLTENRKAFLKEITTIYNRGNK"
     gene            complement(1688..>2185)
                     /locus_tag="VBD27_001998"
     CDS             complement(1688..>2185)
                     /locus_tag="VBD27_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO7025965.1"
                     /translation="LYKKGHKSIQFVTDDLNSEVSEERYLGYFKGARKLGLNQKPALL
                     FDRGNPQVLEEFINRVKEEETTALIVIGDTVSVRVMQFLSFYKLKVPDDISIMTFNNS
                     LFSHLIHPYLSTFDINVNNLGRTSVRRLIDIIKSPDKVFSETIIVPFTLEERESVRDI
                     SKNNN"
BASE COUNT          675 a          434 c          335 g          741 t
ORIGIN      
        1 gttaattctg ataatacttt tccttgttgt ccaacgtaag ttgtaaaatt tcttgtcatg
       61 ataatattcc ttatttattc cctctattat aaatagttgt aatctctttt aagaatgctt
      121 tgcgattttc tgttaagtct tcttttctca tacgccattg ccagttacct ccaacggtat
      181 ttggcatgtt catacggcta tctgcaggtt tatctagtag atcttgcata catgtaatcg
      241 ttgtttgact aacggttgca tacaaggtac gcagtacagt ctcagtaatg ggctcatttg
      301 gcaaacgacg catgtaattt tctgcataag ctttctgttc gactgtaaga ttttcaaacc
      361 agccattaat aacttcgtta tcatgagttc cagcatatgc aattgtattt tctgtgtagt
      421 tatgaggaat atcaatacta tttcctgttg tatcataaaa accgaattcc atgattttca
      481 tacctgggaa ccctgttcca gccagtaatc tctctgctct ctcatcaata tagcctaaat
      541 tctcagcaat aataggtaaa tcacctaatt tttctttgat agttgcaaat aattctggac
      601 cgggagctgg ttgccatgag ccatcattag ctgtttgata atctcctcga atttcccaat
      661 aatctgaaaa acctttaaaa tgatcaatac gcaaatagtc atacatctta acaccagatt
      721 gaatacgata aatccaccag tcaaaatctg actctttgtg gtaatcccaa ttgtaaatag
      781 gattgcccca aagctgacca tcgtcagaaa aatcatccgc tggtacacca gcaatagcaa
      841 gtggttgttt gtcgcaatct aatttaaaca gttcaggcat tgtccaaact tctacactat
      901 cggcagaaac gtagattggc atatcaccga taatttgaat ccctttatca ttagcatatt
      961 cttttaactc aaaccattgt ttgtaaaaga aatattgcgt tacttcatga tattttatca
     1021 cttcactaag cttttgacga tatcctgcta aggcttcttc ttcgcggcgt ataatagcct
     1081 tgtcatccca ttcttgaagc gccttattac caaaatgttc tttgatcgcc ataaattcag
     1141 caaaatcagt tacccatttt tcttcttgca agaaatcaga tagcattctc gtagctctct
     1201 cttcttgcaa gaaattttta actgcttttt ctaaaactgg acggcgtttt tcaaacagac
     1261 cagcataatc aacaacttct ggatcctgtc caaaactaat attttggtag tcatctttcg
     1321 aaatgaaacc ttcaagcgtt agcaaatcaa aatcaattaa atgtgtatta ccagctactg
     1381 cagaaaaaga ctggtaagga gagtccccga agcttgttgt tgttaaggga agaatttgcc
     1441 aaaatttttg atcagtttcg accaaaaaat caacaaaagc gtaggcttct cgtccaaatg
     1501 ttccaatacc taaatcacct ggtagagaag tgatgtgcat taagacacca cttgcacgtt
     1561 ttttcatatt gattctcctt ttcgattact tactttactc ctccatctta acgcaagcga
     1621 ttgcataagt caagcataat ttaaaaaaac ttctatcgaa aattgataga agtctttata
     1681 tcatagctta attattattt ttagaaatat ctcggacact ttcacgctct tctaaagtaa
     1741 aaggcacaat aatagtctca ctaaagactt tgtctggaga tttaatgatg tcaatcagac
     1801 gacgaacgct tgtccttcct agattattaa cattaatatc aaatgtgctt aggtaaggat
     1861 gaatcagatg tgagaagaga gaattattaa aggtcatgat agaaatgtca tctggaactt
     1921 ttaatttata gaaagaaaga aattgcatta ctctaacaga gacagtatca ccaataacaa
     1981 ttaaagcagt tgtttcttct tctttgactc tatttataaa ttcctctaaa acttgcggat
     2041 tgcctctgtc aaataaaaga gctggctttt ggtttaaacc taactttcta gcacctttaa
     2101 aataccctaa atagcgctct tctgatactt ctgaatttaa atcatctgta acaaactgga
     2161 tgcttttatg tcctttttta tagag
//
LOCUS       DATRQA010000205         2074 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.206, whole genome shotgun sequence.
ACCESSION   DATRQA010000205 DATRQA010000000
VERSION     DATRQA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.206"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1017)
                     /locus_tag="VBD27_001999"
     CDS             complement(<1..1017)
                     /locus_tag="VBD27_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7025966.1"
                     /translation="MRIIAITEKVIKELFRDKRTLAMMFLAPILIMFLMNVMFSANSN
                     TKVKIGTINVNTKVVSNLDNIKHIQVRSFKFNSSAKKALKSNKIDALISEDNKSYTVF
                     YANTDSSKTTLTRQAFKTAVNTMNSKELISQVKILANKNPKLAQSLQTRSKYIKEKYN
                     YGNKNTGFFAKMIPILMGFMVFFFVFLISGMALLKERTSGTLDRLLATPVKRSDIVFG
                     YMLSYGILAIIQTIVIVLSTIWLLDIQVVGSIFSVIIVNFILALVALSLGILMSTLAK
                     SEFQMMQFIPLIIMPQLFFSGIIPLENMASWAQTVGKILPLSYSGDALTKIIMYGQGL
                     PNVSS"
     gene            complement(1020..1736)
                     /locus_tag="VBD27_002000"
     CDS             complement(1020..1736)
                     /locus_tag="VBD27_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7025967.1"
                     /translation="MKKVIDLKKLQKAYASETVLNNINLEVFKGEIIGLIGPSGAGKS
                     TLIKTMLGMEKADKGTALVLDTQMPDRNILNQIGYMAQSDALHESLTGLENLLFFGKM
                     KGIQKTELKQQITHISKVVDLENQLDKFVSGYSGGMKRRLSLAIALLGNPTVLILDEP
                     TVGIDPSLRRKIWQELINIKDEGRSIFITTHVMDEAELTSKVALLLRGNIIAFDTPLH
                     LKKQFNVSTIEEVFLKAEGE"
BASE COUNT          687 a          325 c          328 g          734 t
ORIGIN      
        1 tgaagaaacg ttaggtaatc cttgaccgta cataattatt tttgtaagtg catcaccaga
       61 gtaggatagc ggtagaattt taccgactgt ctgagcccaa cttgccatat tttctagagg
      121 gattattcct gaaaagaata attgaggcat tataattaag ggaataaatt gcatcatttg
      181 aaattctgat tttgctagag tagacattaa gattcctaat gataaggcaa ccagagccaa
      241 aataaaattg acaatgatga cactaaaaat actacctaca acttgaatat ctagtagcca
      301 aatagttgat aaaacgataa ctatcgtttg aataattgct agaatgccgt atgataacat
      361 atatccaaat acaatatcac tacgtttaac aggagttgca aggagtcgat caagtgtacc
      421 agaagtacgc tcttttaata aagccattcc agaaattaaa aaaacaaaga agaagaccat
      481 aaatcccatt agtattggta tcatttttgc aaaaaagcct gtatttttat ttccgtaatt
      541 atatttttct ttgatatatt tggagcgagt ttgtaaggat tgtgctagtt tcggattctt
      601 attagctaaa attttaactt gcgaaatcag ttccttactg ttcattgtat taacagcggt
      661 tttaaaagct tgtcttgtta aagtcgtctt tgaagaatct gtattcgcat agaagacagt
      721 ataagattta ttgtcctccg aaataagagc atcaatttta tttgatttga gtgctttttt
      781 agcagatgag ttaaatttaa atgatctcac ttgaatatgc ttaatattat ctaaatttga
      841 aacgaccttc gtgttaacgt taatagttcc aatcttaact tttgtattac tattcgcaga
      901 aaacataaca ttcatcaaaa acataattaa aataggtgct aaaaacatca tagcaagtgt
      961 tcttttatca cgaaacagtt cttttataac cttttcagtt attgcaataa ttctcatgat
     1021 tattctcctt cagcttttaa gaaaacttcc tcaatagtac tcacattaaa ttgttttttt
     1081 aaatgtaatg gagtatcaaa ggcaataatg tttccacgta atagtagtgc aaccttactt
     1141 gttaattctg cttcatccat aacgtgggtt gtaataaaga tagaacgtcc ttcatcctta
     1201 atattaatta gctcttgcca gatttttctc ctcaaggatg gatcaattcc aacggtaggt
     1261 tcatctagga ttaaaactgt ggggtttcca agtagggcga tggctagaga aagccgtctt
     1321 ttcatacctc ctgagtaacc tgagacaaat ttatcaagtt ggttttctag atctactact
     1381 ttagaaatat gagttatctg ctgttttaat tcagtttttt gaataccttt catttttcca
     1441 aagaataata aattttctaa gccagttaaa gactcgtgta aggcatcaga ttgagccata
     1501 tagccaattt gatttaaaat attacgatct ggcatttgag tatcaagaac aagagctgtt
     1561 cccttatctg ctttttccat gccaagcata gttttaatca aggtagattt ccctgctcca
     1621 gagggtccta ttaatccaat tatttctcct ttaaacacct ccaaattaat attatttaaa
     1681 acagtttctg aggcgtatgc tttttgtagt ttttttaaat cgatgacttt tttcatgata
     1741 actttttgct cctctctcag tgagtgagtg tttactcacc aatttgatat catatattat
     1801 agtgtgtaaa gacaggcttg tcaataaaag aattgacagg aagttaatat actataatag
     1861 tcaataaaat ggcttatttg agataactaa ttttttatct taaaaatcag ttgcatggag
     1921 agcattcgtg aaaatgggca cagaataaaa cgactaagaa tatccttgtc gttttatttt
     1981 ttatatagtt atacctcttt taaaattcaa tataataatg tttattcagg tattttttaa
     2041 caatgatatg aagagagaag ctaagtccca gtat
//
LOCUS       DATRQA010000206         2069 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.207, whole genome shotgun sequence.
ACCESSION   DATRQA010000206 DATRQA010000000
VERSION     DATRQA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.207"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            280..1506
                     /gene="clpX"
                     /locus_tag="VBD27_002001"
     CDS             280..1506
                     /gene="clpX"
                     /locus_tag="VBD27_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO7025968.1"
                     /translation="MAGNRNNDMNVYCSFCGKSQDEVKKIIAGNGVFICNECVALSQE
                     IIKEELAEEVLADLAEVPKPKELLEILNQYVVGQDRAKRALAVAVYNHYKRVSYTESS
                     DDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENIL
                     LKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIQINTKNILFIVGGAFDGIEDLVKQRLGEKVIGFGQTSRKIDD
                     NASYMQEIISEDIQKFGLIPEFIGRLPVVAALELLTAEDLVRILTEPRNALVKQYQTL
                     LSYDGVELEFDQDALLAIADKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVRI
                     TKAAVEGTDKPVLETA"
     gene            1517..>2069
                     /locus_tag="VBD27_002002"
     CDS             1517..>2069
                     /locus_tag="VBD27_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO7025969.1"
                     /translation="MAEEFLNTHNASILLSAANKSHYPQDDLPEVALAGRSNVGKSSF
                     INTLLGRKNLARTSSKPGKTQLLNFYNIDDKLRFVDVPGYGYAKVSKTERAKWGKMIE
                     EYLVTRDNLRVVVSLVDFRHDPSADDIQMYEFLKYYEIPVIIVATKADKIPRGKWNKH
                     ESSIKKKLNFDKKDHFIVFSSVDR"
BASE COUNT          651 a          329 c          452 g          637 t
ORIGIN      
        1 attattttga aaaggatgag cagaatgctc atgcttttac tgtgacatat tttgaaaaat
       61 aaggaaggaa tactattcaa tgcaacgtag cctttttggg gtctttaccg catttttagc
      121 tgtgatttgt gttttatgtg caattcctgc tataaagaaa aaaagatatg gattaggaac
      181 agtttttttg atgaatgctt tcactaacct tgtcaacaca atccatgcct tttatggaac
      241 attattttaa attaaatgaa tagaaaagtt gaggaaaaaa tggctgggaa tcgtaataac
      301 gatatgaatg tctattgttc attttgtggc aaaagccaag atgaagtaaa aaaaattatt
      361 gcaggtaatg gtgttttcat ttgtaatgaa tgtgtggcct tatcacaaga aattattaag
      421 gaagaattag ctgaggaagt actggctgat ttagcagaag taccaaaacc taaggaacta
      481 ttagaaatat taaatcaata tgttgtaggg caagatcgtg ctaaacgtgc tttagcagtt
      541 gctgtctaca atcattacaa gcgtgttagt tataccgaga gtagtgacga tgatgtagat
      601 ttgcaaaaat ccaacatttt gatgattggt ccaactggct caggaaaaac cttcttagca
      661 caaacactgg ctaaaagcct taatgtaccg tttgctattg cagatgcgac ttcattgacc
      721 gaagcaggat acgttggaga agatgttgag aatattcttc ttaaattgat tcaagctgct
      781 gattataatg tcgaacgtgc tgagcgtggt attatctacg ttgatgaaat agataaaatt
      841 gctaagaaag gcgaaaatgt ttctatcaca cgtgatgtgt ctggtgaagg tgtacagcaa
      901 gcccttctta aaattattga gggtacggta gcaagtgttc ccccacaggg tgggcgtaaa
      961 catcctaacc aagaaatgat tcaaattaat accaagaaca tcctttttat tgtcggtggt
     1021 gcttttgatg gtattgaaga ccttgtgaag caacgtttag gcgaaaaagt tattggtttt
     1081 ggacagacaa gccgtaaaat tgatgacaac gcttcttata tgcaagagat aatttctgag
     1141 gatattcaaa agtttggact gattccagag tttattggcc gtttaccagt agttgcagcg
     1201 ttagaacttc ttactgcaga agatctggtt cgtattctga cagaaccacg caatgctttg
     1261 gttaaacaat accaaacctt attatcttat gatggtgtag aattggaatt tgaccaggat
     1321 gctctattgg ctatcgctga taaggctatc gagcgcaaga ctggtgcacg tggtttacgt
     1381 tctattattg aagaaacgat gcttgatatc atgtttgaaa ttccaagcca agaagatgta
     1441 acaaaagttc gtatcacaaa ggctgctgtt gagggtactg acaagcctgt tttagagacg
     1501 gcttagggag atagagatgg cagaagaatt tttaaatact cataatgcgt ctattttatt
     1561 gagtgcagct aacaagtcac attatcctca ggatgatctc ccagaagttg ctctcgctgg
     1621 tcgttcaaat gttggtaaat caagctttat caacacgctt cttggtcgta aaaacctagc
     1681 gcgtacgtcg agtaagccag gtaagacaca actgctcaac ttttataaca ttgatgacaa
     1741 actgcgtttt gtcgatgtcc ctggatatgg ctacgctaag gtgtctaaaa ctgagcgtgc
     1801 taagtggggc aagatgattg aagagtacct tgtaacacgt gataaccttc gtgttgttgt
     1861 tagtttagtt gattttcgtc atgatccaag tgcggacgat attcaaatgt atgaatttct
     1921 taagtactat gagattccag taattattgt agcgacaaag gcagataaaa tacctcgtgg
     1981 caagtggaat aaacatgagt cgtctattaa gaagaaatta aactttgata aaaaggacca
     2041 tttcatcgtc ttttcatctg ttgaccgca
//
LOCUS       DATRQA010000207         2055 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.208, whole genome shotgun sequence.
ACCESSION   DATRQA010000207 DATRQA010000000
VERSION     DATRQA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.208"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..2041
                     /locus_tag="VBD27_002003"
     CDS             <1..2041
                     /locus_tag="VBD27_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025970.1"
                     /translation="QDPASIIKKIYLIQNGVPNELKKFDSSFGLTESQIDGYYIYKDA
                     INLKFKLTSGASLKVVYKGQEDPYSHQKEDMTKKGEQLSHSTQANENTAKVTFANIDW
                     SHYSKVTVNGKEVGKGSELPLTKGWTTFVLHKTENSLNVKSLIMETGSVSKKVQQLPL
                     SPRLSKNKHMRDMLLTMQKDSAYYETSDSLVLRINLTADTKLNFNAVKGASALTENMM
                     MRQFAVAGPQDDPVSEHKYPSVFLLTPALLETASEATLNGKEITASGIIGHIKDGDKS
                     KHVEVKMVNENGDMLGTPVIIQGKDLANRTKPLMSGRRVLYAGKQYEFRAKLPLSRFN
                     TWIRVEVVTEAGEKASIVRRMFFDQSVPELNTAVAKRDLTSDTALIHIVAKDDSLKLK
                     LYQDDSLLESVDKTGLYSFRNGVEITKDMTVPLEFGDNIIKLSAVDLSNYRRNETLHI
                     YRNRFDVKASQMTADKGAKVTVDMLMKHLVVPEMAGAYTLTIDEAPNTNESGMLTNAK
                     VSIHYVNGGVDKVDVPIKVVDLEAIRKAEEAHKADEARKAEEARKAEEAHKAEEVRKA
                     EEAHKVEEARKAEEGHKTQEAPIVEEGYKVNNVHQTDTTVKASDLPKTKTVSAVHMAR
                     TDNKQITSHQTHVEKQIKNTLPSTGDSKRGYYITGMAIVMLSVLFSLAKKFKSKY"
BASE COUNT          768 a          320 c          398 g          569 t
ORIGIN      
        1 tcaagaccca gcttcaatta ttaaaaaaat ataccttatc caaaatggtg ttccaaatga
       61 attgaaaaaa tttgactcta gttttggttt aactgaaagt cagatagatg gatactatat
      121 ttataaagat gcaattaacc ttaaatttaa attaaccagt ggtgcaagtc ttaaagttgt
      181 ttataaaggg caagaagatc catatagtca tcagaaagaa gatatgacta aaaaaggtga
      241 acagctcagt cattcaactc aagccaatga aaatacagca aaagtaacct ttgctaatat
      301 tgactggtca cattatagta aggttactgt gaatggaaaa gaagttggta aaggtagtga
      361 gttaccttta actaaaggat ggacaacatt tgtattacat aaaacagaaa attcattaaa
      421 tgttaaaagt ttgattatgg agacgggtag tgtaagtaag aaagttcaac aacttccttt
      481 aagtcctaga ttatctaaaa ataagcatat gagggatatg ctacttacta tgcaaaaaga
      541 ttcagcgtat tacgaaacaa gtgacagtct agtccttcga attaatctca ctgcagatac
      601 taaacttaat tttaatgctg ttaaaggagc gagtgctctt actgaaaata tgatgatgag
      661 acagtttgca gttgctggac cacaagatga tcctgttagt gaacataaat acccatcagt
      721 atttctctta actcctgcct tattggaaac tgctagtgag gcaactctaa atggtaagga
      781 aatcacagca tctggtatta tcggtcacat caaggatggt gataaaagca agcatgttga
      841 agtcaaaatg gtgaatgaaa atggagacat gctaggaacc cctgttatta ttcaaggtaa
      901 agacttggct aatcgaacaa aaccattaat gagtggacgt agagtacttt atgccggtaa
      961 acaatatgag ttccgggcta aattaccact tagtcgtttt aacacttgga ttagggttga
     1021 agtggtaaca gaagcaggag agaaagcaag tattgttcgt cgcatgttct ttgaccaatc
     1081 agttccagag cttaacacag cagttgctaa acgtgatttg acttctgata ctgctcttat
     1141 ccacatcgtt gccaaagatg actctctaaa actaaaatta tatcaagatg attcattact
     1201 tgaatctgtt gataaaaccg gtctttatag ttttagaaat ggtgtagaaa tcactaaaga
     1261 tatgacagta ccactagaat ttggagataa tattattaag ttatctgctg ttgacttatc
     1321 aaattatcgt cgtaatgaga cccttcatat ctatagaaac cgttttgatg ttaaagcaag
     1381 ccaaatgaca gctgacaaag gagctaaagt aactgtggat atgttgatga agcacttagt
     1441 tgttccagaa atggcaggag cttatacatt aacaatcgac gaagctccaa acacaaatga
     1501 atcaggaatg ttaacaaacg ctaaagtatc gattcattat gtaaatggtg gtgttgataa
     1561 agttgatgtt ccgattaaag tagttgactt agaagctatt cgtaaagctg aagaagcaca
     1621 taaagctgac gaagcacgta aagctgaaga agcacgtaaa gctgaagaag cacataaagc
     1681 tgaagaagta cgtaaagctg aagaagcaca taaagtcgaa gaagcacgta aagctgaaga
     1741 gggacataaa acccaagaag cacctatagt tgaagaaggc tacaaagtta ataacgttca
     1801 tcaaactgat actacagtta aagcgtctga tttaccaaag actaagacag tttccgcagt
     1861 tcatatggct agaacagaca ataaacagat aacttcacat cagacacatg ttgaaaaaca
     1921 aattaaaaat acattgccat ccactggtga cagcaaacgt ggttattata tcactggaat
     1981 ggctatcgtt atgctgagtg tattatttag tttagctaaa aagtttaaaa gcaaatatta
     2041 atgataaaat aaaat
//
LOCUS       DATRQA010000208         2052 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.209, whole genome shotgun sequence.
ACCESSION   DATRQA010000208 DATRQA010000000
VERSION     DATRQA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.209"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..715
                     /locus_tag="VBD27_002004"
     CDS             <1..715
                     /locus_tag="VBD27_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025971.1"
                     /translation="SRSNFSSPITLLDYVILFVLFIIGLIPPFIGLIFLVGYAYLVYK
                     KHKQHLNSKYYIIFNLGSSRDYFLYFKDSNFRDKVFDVITKSFNNKNQNIKIDIKNQN
                     IKGDNYESGSSRNDVSGSGNIVGTNIDNGSQVSLSGDISNGSTIIKDSNINNADIHKS
                     VEIPWTDMVEQLNLIINTQLSTADKAIYSELLEISEAEDERKLKNFVNKMSSFFKNDY
                     VQNTITGVLGNVISSILMK"
     gene            733..2031
                     /gene="purB"
                     /locus_tag="VBD27_002005"
     CDS             733..2031
                     /gene="purB"
                     /locus_tag="VBD27_002005"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO7025972.1"
                     /translation="MISRYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEKYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIDELFNPIYYTKRVDDIFERLGLEK"
BASE COUNT          652 a          349 c          446 g          605 t
ORIGIN      
        1 aagtagaagt aatttttcat ctccaataac gttgctagat tatgtgattt tatttgtatt
       61 atttattata ggactgattc ctccttttat aggacttata tttttggtag gctatgctta
      121 cctagtttat aaaaagcata agcaacattt aaattcaaaa tattatatta tttttaactt
      181 gggctctagc agggactatt ttctttattt taaagattcg aattttagag ataaagtttt
      241 cgatgtaatt acaaaatcgt tcaataacaa aaatcaaaat ataaaaattg atataaaaaa
      301 ccaaaatata aagggcgata attatgaatc tggatctagt cgaaatgatg tctcaggtag
      361 tggtaatata gttggaacaa atattgataa cggtagtcaa gttagtttat ctggggatat
      421 atcaaatggt tctactatca taaaagattc taatattaat aatgccgata tacataaaag
      481 tgtggaaatt ccatggactg atatggttga gcagttaaat ttaattatta acactcagct
      541 atctactgcg gataaagcga tatattcaga attgttggaa attagtgagg cagaggatga
      601 aagaaaattg aaaaattttg tcaataaaat gtctagtttc ttcaaaaatg attatgttca
      661 gaataccata actggggtgt taggcaatgt catttctagt attttaatga aataagttaa
      721 aggagacaaa caatgatttc acgttattca cgccctgaga tggcggcaat ttggacagag
      781 gaaaataaat accgtgcttg gttggaggtc gagattttgg ctgacgaggc atgggctgag
      841 ttgggtgaga ttcctaaaga ggatgtggct aagattcgcg agaaggcgga ttttgacatt
      901 gaccgcattc ttgagattga gcaggacacg cgtcacgatg tggtggcttt cactcgtgcg
      961 gtttctgaga cgcttggtga ggagcgcaag tgggtgcact acggtttgac gtcgactgac
     1021 gtggtggaca ctgcctacgg ttacctctac aagcaggcta acgacattat ccgtcgtgat
     1081 cttgagaatt tcacaaacat cgtagctgac aaggctaagg agcacaagtt caccatcatg
     1141 atgggtcgta cccacggtgt tcacgccgag ccaacgactt tcggtctcaa gttggcgact
     1201 tggtacagcg agatgaaacg taatattgag cgttttgaac atgctgccgc aggtgtggaa
     1261 gctggtaaga tttcaggtgc cgttggtaac tttgccaaca ttcctccatt tgtggaaaaa
     1321 tatgtctgtg acaaattggg catccgtccg caagaaattt caacacaggt tcttccacgt
     1381 gacctccacg cagaatattt tgcagtactt gcaagcatcg caacatctat cgaacgtatg
     1441 gcgacagaga ttcgtggtct gcaaaaatca gaacaacgtg aagttgaaga attctttgcc
     1501 aaaggtcaga aaggtagctc tgctatgcct cacaaacgca acccaatcgg ttcagagaac
     1561 atgacagggc tagcgcgcgt gattcgtggt cacatggtga cagcttatga gaacgtgtca
     1621 ctttggcatg agcgtgacat ttcgcactca tcagctgagc gtatcatcac accagacaca
     1681 actatcttga ttgactacat gctcaaccgt tttggcaata tcgttaagaa cttgactgtc
     1741 ttcccggaaa atatgatgcg caatatggaa tcaacttttg gtttgattta tagtcaacgt
     1801 gttatgctca aattgattga aaaaggaatg acacgagaag aagcttatga cttagttcaa
     1861 cctaagacag cttattcctg ggacaatcaa gtggatttca aaccactttt agaagaagac
     1921 accaaagtta cctcttgtct tacacaggaa gaaattgatg aactatttaa tccgatttat
     1981 tacacaaaac gtgttgatga tatttttgaa agactaggat tagaaaaata agaaaattcc
     2041 attactggtt aa
//
LOCUS       DATRQA010000209         2037 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.210, whole genome shotgun sequence.
ACCESSION   DATRQA010000209 DATRQA010000000
VERSION     DATRQA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.210"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            285..1091
                     /locus_tag="VBD27_002006"
     CDS             285..1091
                     /locus_tag="VBD27_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7025973.1"
                     /translation="MSKKIILGILSLLSVVTLVACGSSDKQLQDKVEKKGKLVLAVSP
                     DYAPFEFKALVNGKDKVVGADIELAQAIADELHVKLEVSPMSFDNVLSSLQTGKADMA
                     ISGLSYTKERAKVYDFSKPYYTTENAVLVRKSDLGKYTSIESLKGKKIAVQKGSIEEG
                     VSKKSFKNSHVVSLTAMGEAISELKSGKVEAVDLEKPVAEGYLAQNPDLVLAKFALKT
                     GEGDAKAVALPKDSGKMLKTVNKVIKRLEKEDKYKVYISDAAKLTGNQVE"
     gene            1174..1677
                     /locus_tag="VBD27_002007"
     CDS             1174..1677
                     /locus_tag="VBD27_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEO7025974.1"
                     /translation="MVQGSINIEKYKDLALQKQGEHRKFLASLKKKAPKNLDKIVQQI
                     HQEVFQEIDCTACANCCKTLGPLFTEADISRISKHFRMKLSAFEDLFLEVDEDGDKVF
                     KAMPCPFLGDDNLCSIYDVRPKACREFPHTDRKKIYQINNLTIKNTLTCPAAYLFVEK
                     LKSRIGK"
     gene            1781..1957
                     /locus_tag="VBD27_002008"
     CDS             1781..1957
                     /locus_tag="VBD27_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO7025975.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
BASE COUNT          739 a          274 c          393 g          631 t
ORIGIN      
        1 tttagcaagg agagtgacta aatcgaatta ttatcatttt cttactagac agcaactgta
       61 caaggttctg aaagttagtt gattattaat gatagttagc attttttaag aattggaata
      121 cttttaaaag gtgattgttt taattagtta tagttgttat taaaaatcgt ctaacagcaa
      181 gcatagttat cggtgtaatt tactgaattt ttattcaaaa aaacttgact tatgtataaa
      241 gatgatttat acttgttcct atcataataa aaggaagata gtttatgtca aaaaaaataa
      301 tattaggaat tttatctctt ttatctgtcg ttactttggt ggcgtgtggt tcatcagaca
      361 aacagctaca agataaagtt gagaaaaaag ggaagttagt tttagcggtg agtccagatt
      421 atgctccctt tgagtttaaa gcgcttgtta atgggaaaga taaagttgtc ggcgcagata
      481 ttgagttagc tcaagctatt gctgatgagc ttcatgtcaa actagaggta tctcctatga
      541 gttttgacaa tgttttatca agtttacaaa caggtaaggc tgatatggct atttctggat
      601 tatcatatac taaagagcgt gctaaagtct atgatttttc aaaaccttat tacactacag
      661 aaaatgcggt tcttgttagg aagtctgatt taggaaaata tacttcaatc gaaagcctta
      721 aaggtaaaaa aatagcagta caaaaaggaa gtatcgaaga gggagtttct aagaaatcat
      781 tcaaaaattc tcacgtcgtg tcactgacag cgatgggaga agcgattagt gagttaaaat
      841 caggcaaggt agaagctgtt gatttagaaa aacccgtagc tgaaggttac ttggcccaga
      901 atccagacct tgttcttgct aagtttgccc ttaaaacagg agaaggggat gctaaggctg
      961 tagctttacc taaagacagt ggaaaaatgt tgaagactgt taacaaagtg ataaagagac
     1021 ttgaaaaaga agataaatat aaagtttata tttcagatgc ggctaaatta actggcaatc
     1081 aagttgaata atattaaaaa gcctagtcat aggctttttt ggatgttata atatggcttg
     1141 actaagaata atagcataaa ggcagaggaa tatatggttc agggttcaat taatattgaa
     1201 aaatataaag atttggcgct ccaaaaacaa ggcgagcatc gtaaattttt agcaagttta
     1261 aaaaagaaag ctcctaaaaa cttagataaa atagtccaac agatacatca agaggttttt
     1321 caagaaattg attgtacagc atgtgctaat tgttgtaaaa ctttgggtcc tctatttaca
     1381 gaagctgata tttctaggat ttctaaacat ttccggatga agttatcggc ttttgaagat
     1441 ttatttttag aagtagatga agatggagat aaagtattta aagcgatgcc ttgtccgttt
     1501 ttaggcgatg ataacttatg tagtatatac gatgtcagac ctaaagcttg tcgtgaattt
     1561 ccacatactg atcgtaagaa aatctatcaa attaataact taacgataaa aaatactctt
     1621 acttgtccag cggcatacct ttttgtggaa aaattgaaat cacgtatagg gaaataataa
     1681 cccagagaaa gtatcataaa aatattgaca atctgctttt atttgggtat cctagtaagt
     1741 gaacgtgaaa atgttacaat atcttgagga ggtgaaacat atgtcaaaaa cagtagtacg
     1801 taaaaacgaa tcacttgacg atgcacttcg tcgtttcaaa cgttctgtga ctaaagctgg
     1861 tactcttcaa gaatcacgta aacgtgaatt ctacgaaaaa ccttctgtaa aacgtaaacg
     1921 taaatcagaa gcagctcgca aacgtaaaaa attctaatta gatagaatat gaaaaaacag
     1981 ttcagaattg agctgttttt tcatatatta taactataag ttaataaaaa aactcgg
//
LOCUS       DATRQA010000210         2005 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.211, whole genome shotgun sequence.
ACCESSION   DATRQA010000210 DATRQA010000000
VERSION     DATRQA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.211"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(29..619)
                     /locus_tag="VBD27_002009"
     CDS             complement(29..619)
                     /locus_tag="VBD27_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7025976.1"
                     /translation="MANMYYTENPNVEHDIHELNVKLLGESFSFLTDAGVFSKRMIDY
                     GSQVLLNSLHFEKNKSLLDLGCGYGPLGISLAKVQGVKATMIDINTRALELAKKNATR
                     NGVVVEVFQSNIYENISKTFDYIISNPPIRAGKQVVHSIIEESICYLNTGGSLTIVIQ
                     KKQGAPSAKAKMLDTFGNCDILKKDKGYYILRSEKV"
     gene            725..1645
                     /locus_tag="VBD27_002010"
     CDS             725..1645
                     /locus_tag="VBD27_002010"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO7025977.1"
                     /translation="MNNEFINFDRISRENWKDLHQQSQALLTEKELESIKSLNDNINI
                     QDVIDIYLPLINLIQIYKRSQENLSFSKAIFLKKENYQRPFIIGISGSVAVGKSTTSR
                     LLQLLISRTFKDSHVELVTTDGFLYPNEKLIQNGILNRKGFPESYDMESLLNFLDTIK
                     NGITAKIPIYSHEIYDIVPNQLQTIETPDFLILEGINVFQNQQNHRLYMNDYFDFSIY
                     IDAENKQIEEWYLQRFNSLLQLAEADPSNYYHKFTQIPPHKAMELAKDIWKTINLVNL
                     EKYIEPTRNRADFIIHKGKHHKIDEIYLKK"
     gene            1699..1947
                     /locus_tag="VBD27_002011"
     CDS             1699..1947
                     /locus_tag="VBD27_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEO7025978.1"
                     /translation="MEVKTLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELYRAASSSIDKAASKGLIHTNKASRDKARLATKLG"
BASE COUNT          719 a          355 c          272 g          659 t
ORIGIN      
        1 tattattttt acgctttagg gatatcattt agactttttc acttctcaat atataatatc
       61 ccttatcttt ttttaaaata tcgcaattac caaaagtatc tagcatttta gcttttgcac
      121 ttggagcgcc ttgtttcttt tgaatgacaa tggtaaggct accgcctgta ttaaggtaac
      181 agatactttc ttcgataata gaatgaacaa cttgctttcc agcacgaata ggtgggttgc
      241 ttataatata gtcgaaagtt ttagaaatat tttcataaat gtttgactga aagacttcaa
      301 ctactactcc attgcgtgtt gcatttttct tagctaattc caatgctctc gtattaatgt
      361 caatcatagt tgctttaact ccttgcactt tggctagaga aattcccagc ggaccgtatc
      421 cacacccaag gtctaacaag gatttatttt tttcaaaatg taaactattt agaaggactt
      481 gactaccata atctatcatt cttttagaga aaactcctgc atcagttaaa aaactgaatg
      541 attctcctaa taatttgaca tttagctcat gaatatcatg ttcaacatta ggattctctg
      601 tataatacat attagccata aataattcta accctccaat ttcgccatat tattttacca
      661 tattttgaaa cggttttcaa tcactcaata aaatgttata atagctttca tataggagtt
      721 tgacatgaat aatgaattta ttaatttcga ccgtatttcc agagaaaatt ggaaggactt
      781 acatcagcaa tcccaagctt tactaactga aaaagagctt gaatctatca aaagcttaaa
      841 cgataatatt aatattcaag atgttattga catctacctt cctttaatta accttattca
      901 aatctataaa cgtagtcaag aaaatttatc tttttctaaa gcaatatttc ttaaaaagga
      961 aaattatcaa cgtcctttta ttatcggcat atcagggtca gttgctgttg gaaaatcaac
     1021 taccagtcgc ttactacaat tactaatttc acgaactttt aaagatagtc acgtcgaatt
     1081 agtcactact gatggctttt tatatcctaa cgagaaactc atacaaaatg gaattttaaa
     1141 ccgtaaaggt ttcccggaat cctatgatat ggagtcactt ctaaatttct tagatactat
     1201 aaagaacggt ataaccgcga aaatcccaat ttattctcat gaaatctacg atatcgttcc
     1261 aaatcaattg caaactattg aaacaccaga ttttttaatt ttagaaggaa taaatgtctt
     1321 tcaaaaccaa caaaaccatc gactttacat gaatgattat tttgattttt caatatacat
     1381 tgatgctgaa aataaacaaa ttgaagaatg gtatttacaa cgatttaaca gtcttctaca
     1441 attagccgaa gcagacccta gtaattacta tcataaattc acacaaatac cgccacataa
     1501 agctatggaa ctagctaaag atatttggaa aactattaat ttagtcaacc ttgaaaaata
     1561 tatagaacca accagaaata gggctgattt tattattcat aaagggaaac atcataaaat
     1621 tgatgaaatt tatctcaaaa agtgataaag ggttgccaaa aatcacttaa ttagatatac
     1681 ttatatagtt agattaatat ggaggtgaaa acattggcaa atattaaatc agctatcaaa
     1741 cgcgctgaac ttaacgttaa acaaaacgaa aaaaactcag cacaaaaatc agctatgcgt
     1801 actgctatta aagcatttga agctaaccct tcagaagaac tttaccgcgc tgcttcttca
     1861 agcatcgaca aagctgcttc taaaggttta attcacacaa acaaagcaag ccgcgataaa
     1921 gcacgtcttg caactaaact tggttaatta acctagaaaa catcggatat atcgatgttt
     1981 tttatttaat tttttcgtag aataa
//
LOCUS       DATRQA010000211         1995 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.212, whole genome shotgun sequence.
ACCESSION   DATRQA010000211 DATRQA010000000
VERSION     DATRQA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.212"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            247..1539
                     /locus_tag="VBD27_002012"
     CDS             247..1539
                     /locus_tag="VBD27_002012"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO7025979.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDNKKFKLHLIPSGIFFKEKISVIGNGVVVNPKSLVKELAYLHGEGVTTDNLRISDRA
                     HVILPYHIKLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDREVFAERL
                     KINLAEKNRLFEKMYDSTPLEFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWSEDITACRSLDDLPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWSNI"
     gene            complement(1562..1855)
                     /locus_tag="VBD27_002013"
     CDS             complement(1562..1855)
                     /locus_tag="VBD27_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025980.1"
                     /translation="MILINLIALPICFLAELSAGLLLKLEMHKQHGYYPLERLLMTGS
                     ILTVVSLVLLHTLIHNINSLLFLSIIVVLLISGKSLSLISIYLLKRKSKHQKV"
BASE COUNT          634 a          315 c          403 g          643 t
ORIGIN      
        1 ttaagaatgc tggtatcagt aaatttcaag atttctttat caactataat gttagttttt
       61 taccgttttt ttacacttct aataattatt tttttctttt tttccgataa tatttgagga
      121 atttggcaaa aaaaaacatt ttttgctata ataaggtgtc agacaaactc gcaggtgaaa
      181 ttcctgccat gtttttccag aaaggattgg ggagtcccaa aaaggctcac tcagtgttaa
      241 ataaatatga cttcagttgt tgtagtaggt acccaatggg gtgacgaagg taaaggtaaa
      301 attacagatt ttttatctgc tgacgcagaa gtaattgcac gttatcaagg tggtgataac
      361 gctgggcata ctatcgttat tgataacaaa aaatttaaac ttcatttaat tccttcagga
      421 attttcttca aagaaaaaat ttctgtcatt ggtaatggtg ttgtcgttaa tccgaaatca
      481 ttagttaaag agttagctta tcttcacgga gaaggtgtaa caacggataa ccttcgtatt
      541 tcagatcgtg cgcatgttat cttgccatac cacatcaagc ttgaccaact acaagaagat
      601 gctaaaggtg ataataaaat tggaacaacc atcaaaggaa ttgggccagc ttacatggac
      661 aaggctgcgc gtgtcggtat ccgtattgct gaccttcttg atagagaagt ttttgctgag
      721 cgtttgaaaa ttaatttagc tgagaaaaat cgtttatttg aaaaaatgta tgacagtaca
      781 cctcttgaat ttgatgatat tttcgaagaa tattatgaat atggtcaaca aattaagcaa
      841 tatgtgactg atacatccgt tatcttaaat gatgctcttg atgccggtaa acgtgttctt
      901 tttgaaggag cacaaggagt tatgctcgac attgatcaag gaacataccc atttgtaaca
      961 tcttccaatc cagtagcagg tggtgtcaca attggttcgg gagttggacc aagtaaaatt
     1021 aataaagtgg taggtgtatg taaagcttac actagccgtg ttggcgatgg accattccca
     1081 acagaacttt ttgatgaggt tggtgaccgt attcgtgaga ttggtaaaga gtatggtaca
     1141 acgactggtc gtcctcgtcg cgttggatgg tttgattctg ttgttatgcg tcacagccgt
     1201 cgagtatcag gtattactaa cctctctctg aattcaattg atgttctttc agggcttgat
     1261 acggtgaaaa tttgtgtggc ttatgacctt gatgggaaac gtattgacta ttacccagca
     1321 agccttgaac agctaaaacg ttgtaaacca atctatgaag aattaccggg ctggtctgaa
     1381 gatattacag cttgccgtag cttagatgat cttccagaaa atgcacgtaa ttacgttcgc
     1441 cgtgttggcg aattggttgg tgttcgtatt tctactttct cagtagggcc tggtcgtgaa
     1501 caaacaaaca ttcttgagtc tgtttggtca aatatttaat attaaaaatc tgactgtaca
     1561 gtcagacttt ttgatgttta ctttttcttt ttaatagata gatactaata agacttaagg
     1621 acttcccaga aatcaataaa actactataa ttgataaaaa aagaagtgaa ttgatattgt
     1681 gaatcaaagt atgtaataat actagcgata caactgttag aatactacca gtcatcaata
     1741 agcgttccag aggatagtag ccatgttgct tatgcatttc taattttaat agtagaccag
     1801 ctgacaattc tgctaggaag caaataggta gagctatcaa attaataaga atcatagacc
     1861 aaccaaatcc ttgaccataa cctatatatg atagactatt tttgggaatt gttgacgtgt
     1921 gaattataaa atcaatcgta aagataatgt ctataagaag tagtagcgct aacagataat
     1981 gtagtaagaa gattg
//
LOCUS       DATRQA010000212         1909 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.213, whole genome shotgun sequence.
ACCESSION   DATRQA010000212 DATRQA010000000
VERSION     DATRQA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.213"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            27..227
                     /locus_tag="VBD27_002014"
     CDS             27..227
                     /locus_tag="VBD27_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025981.1"
                     /translation="MNSEIRNRKHVYLNDDIIFQLAEISEFLKSSQSTFKQERSPYSQ
                     IIDLRKEEAQAKSVRNSIEKLL"
     gene            358..1311
                     /locus_tag="VBD27_002015"
     CDS             358..1311
                     /locus_tag="VBD27_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEO7025982.1"
                     /translation="MKICILGLGVIGTTYGYIFQKAGHQVEHLVRESKKQDVPLKLDI
                     TMLDGRYNNKGEEKTDIYTVNLAKPNTNYDFILLSVAGGRIKDAIATLSQNNITGSLL
                     LFCNFWNSREEIEKMVGDFKYIIGFPTAGGNLKQKKLNCVLFDHIMLESEEKATITNY
                     KELLEFLESADIKVENPYDMIEWIWIHMAINAGVTSTAARAGKIDDPKQLAVNLMRDN
                     HGLSIAVKSIRETIKVVKNRGVDLRLYKNELFPYKIPSTIAGIIMKRMFARNELTSRI
                     MTLHNDVKDIMYGCKCIYDAGKQEKLNLPLFYHNMDKIFNS"
BASE COUNT          746 a          209 c          349 g          605 t
ORIGIN      
        1 ccgttaagga aaattaaagg agaaaaatga attcagaaat tcgaaataga aaacatgttt
       61 atttgaatga tgatattatc tttcagttag ctgaaatctc agaattttta aagtcaagtc
      121 agtcaacatt taaacaggag cgtagtccat attctcaaat aatagaccta cgaaaagaag
      181 aagcacaagc taaaagtgtt aggaatagca tagaaaaatt attgtaagaa aacagtgatc
      241 taatacaggt aaggaagtat gagttaggct ataaaactaa gtaaaaaaat atgatataat
      301 ataaaaaatt atcgagtaaa ataaactgga agcgaaactt aaggagatga ttattaaatg
      361 aaaatatgta ttttaggatt aggcgtaata ggcacaactt atggatatat ctttcaaaaa
      421 gcaggacatc aggttgaaca tttagttaga gaaagtaaaa agcaagatgt tccattaaag
      481 ctggatataa ccatgttaga tgggcgatat aacaataagg gagaagaaaa aacagatata
      541 tatactgtga atcttgcaaa accaaataca aattatgact ttatcctttt aagtgttgca
      601 ggcgggagga taaaagatgc catagcaaca ctttcacaaa ataatattac aggaagtttg
      661 ttattatttt gtaacttttg gaacagccgt gaagaaatcg aaaaaatggt tggtgatttt
      721 aaatatatca ttggatttcc gacagcaggt gggaatttga agcagaaaaa gttgaattgt
      781 gttctgtttg accatataat gcttgaaagt gaagaaaaag caactatcac aaactataaa
      841 gagttgttag aatttctcga atctgccgac ataaaagtag aaaatcctta tgatatgata
      901 gagtggatat ggattcatat ggcaataaat gcaggtgtta cttctactgc agcaagggca
      961 ggaaaaattg atgatccaaa acaacttgcg gttaatctta tgagagataa tcatggattg
     1021 tctatcgcgg taaaatcaat aagagaaaca attaaagtag taaaaaatag aggggtcgat
     1081 ttaagattat ataaaaatga actttttcct tataaaatac cttctactat tgcaggaata
     1141 attatgaaac gaatgttcgc aagaaatgaa cttacgagta gaattatgac gttgcacaat
     1201 gatgtaaaag atattatgta tggttgtaaa tgtatctatg atgctggaaa acaggaaaaa
     1261 ttgaacttac cattgtttta tcataatatg gataaaatat ttaatagtta aatccatatt
     1321 cataaatcag tgtttggagg gagaattttt taaatggttt tagtgattta tattttatta
     1381 tgatttacta gtttcagtta caatgattat tgacggacga atgatgtgga atcatctacc
     1441 tgaaaaaatt aatagattaa tgggatatcg tactacttta tcaatgaaaa aataggtaca
     1501 tggagtttta tacatgatta ctgtggctat taattattat tcatattcta ttttatagaa
     1561 gcacagatga tgaaataggt attattagct taattgtagt ttctatacga ataactattt
     1621 taattgtgtt aatacttacg actgaacggg agttgaagaa aactttcaca gaaaattgca
     1681 ttagaaagta gaacgatagc ttctagcttg attaattgaa tagaatattt ttagaaatag
     1741 taaatcacaa aggtttacta ttttttaggg ctttgcttgt tgcaatatat atatctactg
     1801 taatgttata cgaatctaaa ataactcagc ttacttcaat tatagccatg tagcatagaa
     1861 ttttattgag tttttgattt ttgatgacat gagactagat gatcaatat
//
LOCUS       DATRQA010000213         1887 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.214, whole genome shotgun sequence.
ACCESSION   DATRQA010000213 DATRQA010000000
VERSION     DATRQA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.214"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..1808
                     /gene="glmS"
                     /locus_tag="VBD27_002016"
     CDS             <1..1808
                     /gene="glmS"
                     /locus_tag="VBD27_002016"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO7025983.1"
                     /translation="IVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVVGDNKSQLVKS
                     VGRIAELQAKVGDSVSGTTGIGHTRWATHGKPTEGNAHPHTSGSGRFVLVHNGVIENY
                     LQIKETYLTKHNLKGETDTEIAIHLVEHFVEEDNLSVLEAFKKALHIIEGSYAFALID
                     SQDADTIYVAKNKSPLLIGLGNGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKDSVE
                     VQDYDGNVIERGSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRKLISTYANESGDMN
                     VDSDIIKSVQEADRLYILAAGTSYHAGFAAKTMIEKLTDTPVELGVSSEWGYNMPLLS
                     KKPMFILLSQSGETADSRQVLVKANEMGIPSLTITNVPGSTLSREATYTMLIHAGPEI
                     AVASTKAYTAQVATLAFLAKAVGEANGKAEAKDFDLVHELSIVAQSIEATLSEKDVIS
                     EKVEQLLISTRNAFYIGRGNDYYVTMEAALKLKEISYIQTEGFAAGELKHGTISLIED
                     NTPVIALISADSTIAAHTRGNIQEVVSRGANALIIVEEGLEREGDDIIVNKVHPFLSA
                     ISMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
BASE COUNT          626 a          303 c          392 g          566 t
ORIGIN      
        1 gtatcgtagg tgtagttgga aatacaaatg caacggatat tttaattcaa ggcttagaaa
       61 agctagagta ccgaggatat gactcagcag gtatttttgt agtaggtgat aacaaaagtc
      121 aacttgtgaa gtcagtcggt cgaatcgcgg agcttcaggc aaaagtagga gattctgtta
      181 gtggtacgac aggtatcggt catacacgtt gggcaaccca tggtaaacct actgaaggaa
      241 atgcacatcc tcacacttca ggatctggtc gttttgtatt ggtacacaat ggtgttattg
      301 aaaattacct tcaaattaaa gaaacttact taacaaaaca taacctcaag ggtgaaactg
      361 atacagaaat agctattcat ttagttgaac actttgttga agaagataac ttatcagtgc
      421 ttgaagcctt taaaaaagcc ttacatatta tagaaggatc atacgcattt gctcttattg
      481 attctcaaga tgctgatacg atttacgtag ctaaaaataa atcacctctt ctaataggtt
      541 tgggaaatgg ttataatatg gtgtgttcag atgccatggc tatgatacgt gagacaagtg
      601 aatatatgga aatccacgat aaagaactcg ttattgttaa aaaagatagc gttgaagttc
      661 aagattatga tggtaatgtc attgaacgtg gtagctacac agcagagctt gacttatcag
      721 atattggtaa ggggacttat ccattctaca tgcttaaaga aattgatgag caaccaactg
      781 tgatgcgtaa gttgatttca acttatgcga acgaatctgg agatatgaat gtggattctg
      841 atattataaa atcagtccaa gaagcagatc gtctttacat attggctgca ggaacgtcat
      901 atcatgctgg ttttgctgct aagaccatga tcgaaaaatt aactgataca cctgttgaac
      961 ttggagtttc ttcagaatgg ggttacaaca tgccactttt gagtaagaag ccaatgttta
     1021 ttttacttag tcaatcggga gaaacagcag atagtagaca agttttagtt aaagcaaacg
     1081 aaatgggaat ccctagttta actataacaa atgtgcctgg ctcaacactt tctcgtgaag
     1141 caacctatac gatgctcatt catgcaggac ctgaaatcgc tgttgcttct acaaaagcat
     1201 atactgctca agttgcaacg ttagctttct tagcaaaagc ggtaggagaa gcaaatggta
     1261 aggcagaagc aaaagatttt gatttggttc atgaattgtc aattgtagca caatcaattg
     1321 aagcgacctt atcagaaaaa gatgttatct ctgagaaggt agagcagttg ttaatctcga
     1381 cacgtaatgc cttctacatt ggtcgtggta acgactatta tgttacaatg gaggctgcac
     1441 tgaagctaaa agagatttct tatattcaga cagaaggttt tgctgcaggt gaattaaaac
     1501 atggtacaat ttcgttgatt gaagacaata ctcctgttat tgctttaata tctgctgatt
     1561 caactattgc agcgcatact cgtggaaata tccaagaagt tgtttctcgc ggagcaaatg
     1621 ctttgattat tgtagaagaa gggttggaac gtgaaggtga tgatattatt gttaataaag
     1681 ttcacccatt cttatcagcc atttcaatgg ttattccgac tcaattaatt gcttactatg
     1741 cttcattaca acgtggctta gatgttgata aaccacgtaa tttggcaaaa gctgtaactg
     1801 ttgaatagac tcgttacact aaaatacgct aatattaact acaaaatatt aaaaattgta
     1861 aaaaagccta tttttgaagg aaaatag
//
LOCUS       DATRQA010000214         1881 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.215, whole genome shotgun sequence.
ACCESSION   DATRQA010000214 DATRQA010000000
VERSION     DATRQA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.215"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            72..200
                     /locus_tag="VBD27_002017"
     CDS             72..200
                     /locus_tag="VBD27_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEO7025984.1"
                     /translation="MAFGDNGQRKKTGFEKLTLFVVILMVLVTVGGLVFGAISAIM"
     gene            261..1574
                     /gene="obgE"
                     /locus_tag="VBD27_002018"
     CDS             261..1574
                     /gene="obgE"
                     /locus_tag="VBD27_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO7025985.1"
                     /translation="MSMFLDTAKISVKAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFKVNEGLRTLMDFRYNRNFKAKAGEKGMTKGMHGRGAEDLIVSLPPGTTVRDANT
                     GKVITDLVEHDQEFVVARGGRGGRGNIRFATPRNPAPEIAENGEPGEERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVVSAAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYDDYISINNELETYNLRLME
                     RPQIIVANKMDMPDSEENLAAFKEKLAANYDEFDDMPMIFPISSLAHQGLENLMDATA
                     KLLANTEEFLLYDETDMQEDEAYYGFNEDERPFEITRDDDATWVLYGDKLEKLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDIVRIGNFEFEFVD"
     gene            1600..1758
                     /locus_tag="VBD27_002019"
     CDS             1600..1758
                     /locus_tag="VBD27_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025986.1"
                     /translation="MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLER
                     EKLAKEKG"
BASE COUNT          582 a          270 c          449 g          580 t
ORIGIN      
        1 gtagtttgcg aaataagacg tttcgtgtta atatagactt aactatatat tcagtaaatg
       61 aggtgacatg agtggctttt ggagataacg gacaacgaaa aaaaactggg tttgaaaaac
      121 taactctttt tgttgttatt ttgatggtac tagtaacagt tggtggtttg gtatttggtg
      181 ctattagtgc aatcatgtaa tgagaaacgc tgtaaagcgt ttttttatac tgaatagaat
      241 aaagaatagg aattttagaa atgagtatgt ttttagatac tgccaaaatt agtgttaagg
      301 caggtcgcgg tggtgatggc atggttgctt ttcgccgtga gaaatatgtc cccaatggag
      361 gcccgtgggg cggtgatggc ggtaaaggcg gatcggtcat ttttaaagta aacgaaggtt
      421 tacgaacttt gatggatttt cgatataacc gtaattttaa agcaaaagct ggtgaaaaag
      481 ggatgactaa aggtatgcac ggtcgaggtg cagaagacct tattgtgtca ttaccacctg
      541 gaacaactgt tcgtgatgct aatacaggta aggttattac cgaccttgtg gagcatgatc
      601 aggaattcgt tgtagccaga ggtggccgcg gtggtcgtgg aaatattcgc tttgcaaccc
      661 cacgtaatcc agctcctgaa attgctgaaa atggggagcc tggcgaagaa cgtgaattac
      721 aattagaatt aaaaattttg gctgatgttg gtctcgttgg tttcccatca gttgggaagt
      781 cgacgctact tagtgttgtt tcagcagcaa agcctaaaat tggtgcctat cattttacga
      841 caattgtccc taatttaggg atggttcgta caaaatcagg tgatagcttt gcaatggctg
      901 atctccctgg tttgattgaa ggggctagtc aaggtgtcgg attaggaact cagttcctta
      961 gacatattga gcgtacacgt gttattctac atgttattga tatgtcggca agtgaaggac
     1021 gagaccctta cgatgactat atttcaatta ataatgagtt ggaaacttac aatcttcgat
     1081 tgatggaacg tccgcaaatt attgttgcca ataaaatgga tatgcctgat tcagaagaga
     1141 atttggcagc attcaaagaa aaattagctg caaactatga tgagtttgat gacatgccta
     1201 tgatttttcc aatttcaagt ttagcacatc aaggactaga gaaccttatg gatgcgacgg
     1261 ctaaattatt agccaataca gaagagtttc ttctttatga cgaaactgac atgcaagaag
     1321 atgaagcata ctatggattt aacgaagatg aacgtccatt tgagattaca cgtgatgatg
     1381 atgcaacgtg ggtattgtat ggtgataagc tagaaaaact ctttgttatg acaaatatgg
     1441 aacgcgatga atcaataatg aaatttgctc gtcagttacg tggtatgggc gttgatgaag
     1501 cacttcgaga gcgcggagct aaggatggag atattgttcg tataggtaac ttcgaatttg
     1561 aatttgtaga ttaatgcagt acttgattgg aggtttttta tgggagataa accgatatca
     1621 tttcgtgata aagatgggaa ctttgtttca gcggccgatg tctggaatgc tgaaaaatta
     1681 gaagaactct ttaacacatt aaatcctaat cgtaaacttc gtttagaacg cgaaaaatta
     1741 gcaaaagaaa aaggatagtt aagtaactat cctttttagt tattttgggt atgaaaaaag
     1801 actctaaaac ataattgata gagtgttaag gtacagatta ctttattatt ttaaacgttt
     1861 ttgagctttt tgatatactg a
//
LOCUS       DATRQA010000215         1873 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.216, whole genome shotgun sequence.
ACCESSION   DATRQA010000215 DATRQA010000000
VERSION     DATRQA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.216"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1024)
                     /gene="trpS"
                     /locus_tag="VBD27_002020"
     CDS             complement(<1..1024)
                     /gene="trpS"
                     /locus_tag="VBD27_002020"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO7025987.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQNEGSYTLFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTLFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPAGFLVYPVAQAADITAFKANLVPVGTDQKPMIE
                     QTREIVRSFNHAYNCQVLVEPEGIYPENDAAGRLPGLDGNAKMSKSLNNGIFLADDMD
                     TVKKKVMSMYTDPNHIKVEEPGQIEGNMVFHYLDVFGRDEDQKEITAMKEHYQKGGLG
                     DVKTKRYLLDILERELSPIRERRLEYAKDMGQVYQMLQKGSEKAQAVAASTLDEVKSA
                     MGLNYFK"
     gene            complement(1132..1824)
                     /locus_tag="VBD27_002021"
     CDS             complement(1132..1824)
                     /locus_tag="VBD27_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO7025988.1"
                     /translation="MRRDDIIMPQDGRKMAVLLGFIGGFIDVYSHMQFHSLVATQTGN
                     IILMVANLGQNDFHFFITKLFSIFFFSIGFLVGIVIKERAVTAFWRLYALCPLLLSTM
                     FIPFVENVNHNIWVALLAFSTGILMLTFSGSRIESEPYTILMTSGNYRKMLQFWYEYI
                     VNRQRTAMQKRRAINYSLVVLAFIIGALVAAIVYDIFAYRAILGVTITLLIIMIHYTI
                     EIIKNDLTLHNV"
BASE COUNT          622 a          364 c          269 g          618 t
ORIGIN      
        1 atttaaaata gttcaaaccc atggctgatt taacctcatc caaagtagag gctgcaacag
       61 cttgagcttt ctcactacct ttttgtaaca tttgataaac ttgtcccata tctttagcat
      121 attcaaggcg acgttctcta ataggtgata attcacgctc taagatatca agtagataac
      181 gttttgtttt aacatctcca agtccacctt tttgatagtg ttctttcata gcagtaatct
      241 ctttttggtc ttcatctctt ccaaaaacat caaggtaatg gaataccata ttaccttcga
      301 tttgtcctgg ttcttctact ttaatatggt ttggatcagt atacatgctc ataacttttt
      361 tcttaactgt atccatatca tctgctaaaa agattccatt atttaatgac ttagacattt
      421 ttgcattacc atctaaccct ggcagacgac ctgctgcatc attttctgga tagattccct
      481 ctggttcaac taaaacctga caattataag catgattgaa agatctaact atttcacgtg
      541 tttgttcaat cattggtttt tggtcagtcc ctacaggaac aaggttagct ttaaaagcgg
      601 ttatatcagc tgcttgtgca acaggataaa ctaagaatcc tgctggaata gactcaccaa
      661 aacctttttg agcaatttct gtttttacag ttggattacg ttccaaacgt gcaagtgaaa
      721 ccaagttcat ataatacata cttaattctg ctaactcagg aatttgactt tgaatgaata
      781 aagtcgactt gtttggatcc agcccaacag ctaaataatc aagtgcaaca ttaccaattg
      841 attcaacaat agtttgcgga tctttagcat ggtctgtcag tgcttgttgg tctgctaaaa
      901 agacaaataa agtatagcta ccctcatttt gaagtagaac tctatttttt aagctaccta
      961 cataatgtcc aatatgcaac tttcctgtcg gtctatcacc tgttaagata attggttttg
     1021 tcatgtttac tccttaatat accaaagata tcatttcata tagttatctt atcatgtcac
     1081 aagccctatt gcaattcatc cctacaagaa acattcacct tattaagatg attaaacatt
     1141 atgtaacgtt aaatcgttct taataatttc aatcgtataa tgaatcataa taattaaaag
     1201 cgttatcgtc acacctaaaa tagctctata tgcaaagata tcgtaaacta ttgcagctac
     1261 caatgctcca ataataaatg ctagaactac cagtgaataa ttgatagctc gtcttttttg
     1321 catagccgtt ctctgacgat taacaatata ctcataccaa aattgtagca ttttccgata
     1381 attaccagaa gtcattaaga tagtgtatgg ttcagattca atgcgacttc ctgaaaatgt
     1441 caacattaaa atacctgtgc taaaggctaa taaagctacc cagatattgt ggtttacatt
     1501 ttcaacaaaa ggaataaaca tcgtagaaag taaaagggga cataatgcat atagacgcca
     1561 aaaagctgtt actgcacgtt ctttaatcac aataccaacc aaaaaaccaa ttgaaaaaaa
     1621 gaaaatagaa aataatttag tgataaaaaa gtgaaaatca ttttgtccga gatttgccac
     1681 catcaagata atattacctg tctgtgttgc aacaagggag tgaaactgca tgtgactata
     1741 aacgtcaata aaaccaccaa taaaacccaa taatacagcc atttttcttc catcttgtgg
     1801 cattataata tcatctctgc gcattatagc aaattctcct tcttgtatta tcatgttttc
     1861 tattatatac caa
//
LOCUS       DATRQA010000216         1797 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.217, whole genome shotgun sequence.
ACCESSION   DATRQA010000216 DATRQA010000000
VERSION     DATRQA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.217"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..364
                     /locus_tag="VBD27_002022"
     CDS             <1..364
                     /locus_tag="VBD27_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025989.1"
                     /translation="LLIFSGGFYMKEQQRKEELKRNREYEVSLVKALKNSYSDIKEIQ
                     ISNPITTTMPGDWRSTVKIIFSDNDEITYRITHNKDDEINRSGSINEKEHLFLSKKYG
                     RTKNHILVEFSTGEKEKR"
     gene            364..951
                     /locus_tag="VBD27_002023"
     CDS             364..951
                     /locus_tag="VBD27_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025990.1"
                     /translation="MTYSYSEYDLNRLNQGENVYSVNPNYVKQNGGKIITANPKKSNE
                     TNLIVTKTGKAFKVLKTSPDDMSGYQGMAVAPIIKGKVDYNSVAVISAATDSSNYKDL
                     IGAVSSAQPHQSSTQLKSADKFLKDVQSHDKWTVTQLSGYSQSAYMLKLGAKYHIPTT
                     VFNGWFRYSTLNEDEKNSWLSILNILLIFDIKRIM"
     gene            897..1577
                     /locus_tag="VBD27_002024"
     CDS             897..1577
                     /locus_tag="VBD27_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025991.1"
                     /translation="MAKHPEYFANFRHKEDNVTWWNDFNKLDDKGYGTVKWVNGKSHK
                     IESWKFTDDGQLKDEKGNIVNPKSPAVQSVLYEEVHFQKAKAKLKKSGGKLSHSEKVY
                     LDSEQAIFIANGLTTASQTASDDIKKNAELAKEKASELFAKTKVMPPGITDLSPEELA
                     DAYSAGGVREDTIVTPIETFFDEKVTNAQEITTSYTNLQKQIESGVQKLLEEDSKLAG
                     EFKEWSQY"
     gene            1562..>1797
                     /locus_tag="VBD27_002025"
     CDS             1562..>1797
                     /locus_tag="VBD27_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7025992.1"
                     /translation="MVTILDQNENKINSLKINLGKLEDNYHRENMEIEDKLYSLEDKK
                     NELETYLQEVYEETSHFLRQNDSDGSTFMSLNRI"
BASE COUNT          723 a          241 c          330 g          503 t
ORIGIN      
        1 actacttatt tttagcggag gattttatat gaaagaacaa caaagaaaag aagaactaaa
       61 acggaatcga gaatatgagg ttagtctagt caaagcattg aaaaattcat attcagatat
      121 taaggagatt caaatttcca atccaataac aacaacaatg ccaggtgatt ggcgtagtac
      181 tgtgaaaatt attttttcag ataatgatga aatcacttac cgtataaccc ataataaaga
      241 tgatgaaatt aatcgcagtg gatcaataaa tgaaaaagaa catttatttt taagtaaaaa
      301 atatggaaga acaaaaaatc atattttagt tgagttttct acaggagaaa aggaaaaaag
      361 ataatgacat attcttatag cgagtatgat ttaaatagat tgaatcaagg tgagaacgta
      421 tatagtgtaa atccaaacta tgtgaaacaa aatggcggaa aaataataac agctaatcca
      481 aaaaaatcta atgaaacaaa tttaatagta acaaagacgg gtaaagcgtt taaggtatta
      541 aaaacttctc ctgacgatat gtctggctat cagggtatgg cagttgcacc aattatcaag
      601 ggtaaagtag attataacag tgttgcagtt atttccgccg caacggattc aagtaattat
      661 aaagatttaa ttggcgcagt ttcatctgct caaccacatc aaagttcgac tcagttaaaa
      721 tcagctgata aatttttaaa agacgtacag tcacatgaca aatggactgt gactcagctt
      781 tcaggctatt ctcaatctgc ttatatgcta aaattaggtg ctaaatatca tatcccgact
      841 acagtattta atggatggtt taggtatagc actttaaatg aagatgaaaa aaattcatgg
      901 ctaagcatcc tgaatatttt gctaattttc gacataaaga ggataatgta acttggtgga
      961 acgattttaa taaactagat gataaaggtt atggtaccgt gaaatgggta aatggtaaaa
     1021 gtcataaaat agagagttgg aaatttactg atgatggtca gttaaaagat gaaaaaggta
     1081 acatcgtaaa tcctaagtca ccagctgttc agtctgttct ttatgaagaa gttcattttc
     1141 aaaaagctaa agctaaatta aaaaaatctg gaggcaaact tagtcatagt gaaaaagtat
     1201 atttagattc tgaacaggct atatttattg ctaacggttt aaccacggca tctcaaacag
     1261 cttctgatga tatcaagaag aatgctgaat tagctaaaga aaaagcatca gaactatttg
     1321 caaaaactaa agtgatgcct cctggtataa cagatttaag tccagaagaa ttagctgatg
     1381 catatagtgc gggtggagtt agagaggaca ccatagtaac accaattgaa actttctttg
     1441 atgaaaaagt tactaatgca caagagatca caacttcata tactaatctt caaaaacaaa
     1501 ttgaatctgg tgtccaaaaa ttgctagaag aggactccaa attagcagga gaatttaagg
     1561 aatggtcaca atactagatc aaaatgaaaa caaaattaat agtctaaaaa taaacctagg
     1621 gaaattggaa gataattatc atcgcgaaaa tatggagata gaagataaat tatatagcct
     1681 tgaggataaa aagaacgagt tagagactta tttacaagaa gtctatgaag agacaagtca
     1741 ctttttgaga caaaatgatt cagatggttc gacttttatg agtttaaatc gaataat
//
LOCUS       DATRQA010000217         1796 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.218, whole genome shotgun sequence.
ACCESSION   DATRQA010000217 DATRQA010000000
VERSION     DATRQA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.218"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            9..1778
                     /gene="uvrC"
                     /locus_tag="VBD27_002026"
     CDS             9..1778
                     /gene="uvrC"
                     /locus_tag="VBD27_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO7025993.1"
                     /translation="MKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVKSYFHGS
                     HNTKTELLVSEIEDFEYIVTTSNTEALLLEINLIQENMPKYNIRLKDDKSYPYIKITN
                     ERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCFYYHL
                     GQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERAAEYR
                     DLIEAISLLRTKQRVIHQDMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNMFPYY
                     NEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETIVGCKIVKPQRGEKKQLVN
                     LAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGTSPVA
                     AMVVFVNGNPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLIVIDG
                     GQGQVNIARDVIENQLGLAIPIAGLQKNDKHQTHELLFGDPLEVVELPRNSEEFFLLH
                     RIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSLPNIQKADIED
                     IIMCGIPRTVAESLRDSLNDPPK"
BASE COUNT          621 a          263 c          353 g          559 t
ORIGIN      
        1 atgaactgat taaacataag ctagaattgt taccagacag tcctggttgt taccttcata
       61 aagataagaa tgggacaatt atttatgtag gtaaggctaa aaatcttaag aatcgtgtga
      121 aaagttattt tcatgggagt cataatacta agacagaact tttggtctct gagattgaag
      181 actttgaata cattgtgaca acatctaata ccgaagctct attattggaa attaatttaa
      241 tccaagaaaa tatgccgaag tataatattc gattgaaaga tgataagtct tacccatata
      301 tcaaaataac aaatgaacgt tatcctcgtc ttatgattac acgtcaagta aaaaaatcag
      361 atggcactta ttttggacct tatccggatt ctggggcagc aactgagatt aagcggttac
      421 tagataggct ctttcctttt aagaaatgta ctaatccagc aaataaagta tgcttttatt
      481 atcatttagg gcaatgcaat gcacatacag tttgtcaaac taataaagct tattgggata
      541 gtctgagaga agatgtaaaa cagtttctta atggtaaaga taataaaatt gttaacggat
      601 taactgaaaa aatgaagagc gctgctatga ctatggaatt tgagcgtgcg gctgaatatc
      661 gagatttgat cgaagcaatt agtttgttgc gtactaagca gcgtgttatt catcaagata
      721 tgaaggatag agatgttttt ggttatttcg ttgataaggg ttggatgtgt gtacaggtct
      781 tttttgttcg aaatggaaaa ttgattcagc gtgacgtgaa tatgtttcct tattataatg
      841 aaccagagga agattttcta acttacatag ggcaatttta tcaagataca aaacattttc
      901 tacccaaaga agtctttatt ccacaggata ttgatgctaa gtcggttgaa acaattgtag
      961 gctgtaagat tgttaaacca caacgtggtg aaaagaaaca gttagtaaat ctggcaataa
     1021 aaaatgctcg tgtcagttta caacagaagt ttgacttact ggaaaaagat attcgtaaaa
     1081 ctcatggggc aattgaaaat ttaggtaatc ttcttaatat ccctaaacca gtacgcatag
     1141 aagcatttga taattccaat attcaaggga caagtccagt agctgctatg gtggtatttg
     1201 tgaatggaaa tccaagtaaa aaagactatc gaaagtttaa aattaagact gttatcggac
     1261 cagatgatta tgctagtatg agagaagtca ttcatcggcg ttatagtagg gttttaaaag
     1321 atggcttgac acctccggac ttgattgtaa ttgatggtgg tcaggggcaa gtaaatatag
     1381 cgcgtgatgt tattgagaac caattaggtt tagccatacc tattgcaggt ttgcaaaaga
     1441 atgataaaca ccaaacacat gaattacttt ttggagatcc tttagaagta gtagaattac
     1501 cgcgtaattc agaagaattt ttcttacttc accgtattca agatgaggta catcgttttg
     1561 ctattacttt tcatcgtcaa ttacgctcca aaaattcctt ttcttcaaaa ttagatggta
     1621 tcactgggtt gggaccaaaa cgtaaacaat tgctaatgaa acattttaaa agcttaccta
     1681 atatccaaaa agctgatatt gaggacatta ttatgtgtgg tattcctagg acagttgcag
     1741 aaagtctccg agattctctt aatgatcctc ctaaataaaa aacttagtag aacagt
//
LOCUS       DATRQA010000218         1795 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.219, whole genome shotgun sequence.
ACCESSION   DATRQA010000218 DATRQA010000000
VERSION     DATRQA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.219"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1448)
                     /gene="gtfA"
                     /locus_tag="VBD27_002027"
     CDS             complement(<1..1448)
                     /gene="gtfA"
                     /locus_tag="VBD27_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEO7025994.1"
                     /translation="MVVYNLNRGIGWASSGVEYAQAYRSEVFRKLGVEAKFIFTDMFQ
                     NENIEHLTRNIGFEDNEIIWLYTFFTDLTIAATSYSLQQLKESFSLPIDRTEKNGKII
                     SFFFKGSSIVVTVMLNDESSNIVQRVEYLMGGKLVRKDYYSYTKMFSEYYAPEDIGPC
                     LYQRTFYNEDGSVAYEENVDGENSIFKFKETILYSKEELVGYMLEKLQLTNSDLILLD
                     RSTGIGQAVLRNKGNAKVAVVVHAEHYNVSATDETTILWNNYYDYQFSNADSIDAFIT
                     STETQTKTLIDQFKKYLNIEPVVYTIPVGSLSKLQRKEWHERKAFSLLTCSRLASEKH
                     IDWLINAVVEANKVIPELTFDIYGEGGERQKLQEIIAKNKANNYIRLMGHKNLSSVYK
                     DYQVYLSGSTSEGFGLTLMEAIGSGLPIIGLDVPYGNQTFIENNLNGYLIPRETPDNP
                     QQISTAFAQYIVALFNSKDICKKHEYSYRIAS"
     gene            complement(1464..>1795)
                     /locus_tag="VBD27_002028"
     CDS             complement(1464..>1795)
                     /locus_tag="VBD27_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO7025995.1"
                     /translation="KLLIDIFDREFTAKKTKLQSDNEFDNFVRISILKAIDKSWIEEV
                     DSLQQLKGVVTNRGIGQRDIIQEYYKESLNSYYKMGKEIRYSIVKNIMLSTISKASDG
                     SYSIYYV"
BASE COUNT          581 a          344 c          222 g          648 t
ORIGIN      
        1 ctactagcta ttctataaga atactcgtgt tttttacaaa tatctttaga attaaataaa
       61 gcaacaatat actgagcaaa agcagttgat atttgttgag gattatctgg tgtttccctt
      121 ggaataaggt agccattcaa attattttca ataaaggtct gattaccata cggaacatct
      181 aaaccaatta tcggaagccc cgatccgatt gcctccatca aggttaaacc aaaaccttca
      241 ctggtagaac ccgataaata aacttgataa tctttataaa cagaagatag gtttttatgc
      301 cccattaatc gtatataatt atttgcctta tttttagcaa ttatttcttg taacttttgc
      361 cgttcgccac cttcaccata aatatcaaag gttaattcag gaattacttt attagcttct
      421 accacagcat taattaacca atcaatatgt ttttccgaag ctagacgtga gcaagttaac
      481 aacgaaaaag cttttctttc atgccactct tttctctgta attttgataa acttcctaca
      541 ggaattgtat aaacgaccgg ttcaatattt aaatactttt taaattgatc aatcaacgtc
      601 ttagtttgcg tttctgttga ggtgataaaa gcatctatgc tatcagcatt tgaaaattga
      661 tagtcataat aattattcca taaaatcgta gtttcatcag ttgcgctaac attataatgc
      721 tctgcatgaa caacaacagc tactttagca tttcctttat tccttagaac agcttgccca
      781 atgcctgtag agcggtctaa caagattaga tcactattcg ttaattgtaa tttttctaac
      841 atgtatccaa ctaattcttc tttagaatat agtatagttt ctttgaattt aaaaatacta
      901 ttctcgccat ctacattttc ttcatatgca acacttccat cttcattata aaatgtacgc
      961 tgatacaggc atggacctat atcttcggga gcataatatt ctgaaaacat tttagtatat
     1021 gagtagtagt cttttctaac aagctttccc cccataagat actctaccct ttggacgatg
     1081 ttactagatt catcgttaag cataactgta actacaatac tacttccttt aaaaaaaaat
     1141 gatattattt taccgttttt ctcagtccta tcaatcggta gtgaaaaact ttctttcaat
     1201 tgttgcagtg aataagaagt tgcagcaatt gttaaatcag taaaaaaagt ataaagccat
     1261 ataatctcat tgtcttcaaa gccgatattt cgagtaaggt gctctatatt ttcattctga
     1321 aacatatcag taaaaataaa tttagcttca acacctaact ttctaaaaac ttctgaacga
     1381 tatgcttgag catactctac accgctactt gcccaaccta tccctctatt caaattataa
     1441 actaccatta actccgtttt cctttataca taatatattg agtaacttcc atcacttgct
     1501 tttgaaattg tacttaacat aatattctta acgatagaat atcttatttc ttttcccatc
     1561 ttatagtaag aattaagcga ttctttataa tattcttgaa taatatctcg ttgaccgata
     1621 cctctattgg ttacaactcc ctttaattgc tgtaggctat ccacttcttc tatccacgat
     1681 ttgtcaatag cctttaatat cgatattctg acgaaattat caaattcatt atcactttga
     1741 agtttagttt tctttgcagt aaattctcta tcaaatatgt caatcaataa tttaa
//
LOCUS       DATRQA010000219         1789 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.220, whole genome shotgun sequence.
ACCESSION   DATRQA010000219 DATRQA010000000
VERSION     DATRQA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.220"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..900
                     /locus_tag="VBD27_002029"
     CDS             <1..900
                     /locus_tag="VBD27_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO7025996.1"
                     /translation="ILDSSEKDCYDLKHNSLRIINKDALTSELTCLTVDQAFINIPIE
                     LFGKYQIQDIINDIEAMGVIVNVNVEALSFDNIGEKRIQTFEGYSVITYSMKFYKYSH
                     LIAKRFLDITGAIIGLLICGIVAIFLVPQIRKDGGPAIFSQNRVGRNGRIFRFYKFRS
                     MRVDAEQIKKDLLVHNQMTGLMFKLEDDPRITKIGKFIRKTSIDELPQFYNVLKGDMS
                     LVGTRPPTVDEYEKYNSTQKRRLSFKPGITGLWQISGRNNITDFDEIVKLDVQYINEW
                     SIWSDIKIILLTLKVVLLGTGAK"
     gene            924..1373
                     /locus_tag="VBD27_002030"
     CDS             924..1373
                     /locus_tag="VBD27_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEO7025997.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            1373..>1789
                     /locus_tag="VBD27_002031"
     CDS             1373..>1789
                     /locus_tag="VBD27_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEO7025998.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMSVISLGKLPVVVPRRKQFGEHIND
                     HQIQFLKKIAHLYPLAWIEDVDGLAEALKRNIATEKY"
BASE COUNT          617 a          246 c          361 g          565 t
ORIGIN      
        1 atcttggact cctctgaaaa ggattgttat gatttgaaac ataactcgtt aaggataata
       61 aacaaagatg ctcttacttc agagttaacc tgcttaactg ttgatcaagc ttttattaac
      121 atacccattg aattatttgg taaataccaa atacaagata ttattaatga cattgaagca
      181 atgggagtga ttgtcaatgt taatgtagag gcacttagct ttgataatat aggagaaaag
      241 cgaatccaaa cttttgaagg atatagtgtt attacatatt ctatgaaatt ctataaatat
      301 agtcacctta tagcaaaacg atttttggat atcacgggtg ctattatagg tttgctcata
      361 tgtggcattg tggcaatttt tctagttccg caaatcagaa aagatggtgg accggctatc
      421 ttttctcaaa atagagtagg tcgtaatggt aggattttta gattctataa attcagatca
      481 atgcgagtag atgcagaaca aattaagaaa gatttattag ttcacaatca aatgacaggg
      541 ctaatgttta agttagaaga tgatcctaga attactaaaa taggaaaatt tattcgaaaa
      601 acaagcatag atgagttgcc tcaattctat aatgttttaa aaggtgatat gagtttagta
      661 ggaacacgcc ctcccacagt tgatgaatat gaaaagtata attcaacgca gaagcgacgc
      721 cttagtttta agccaggaat cactggtttg tggcaaatat ctggtagaaa taatattact
      781 gattttgatg aaatcgtaaa gttagatgtt caatatatca atgaatggtc tatttggtca
      841 gatattaaga ttattctcct aacactaaag gtagttttac tcgggacagg agctaagtaa
      901 aggtaaggtt tgaaaggaat ataatgaaaa tttgtctggt tggttcaagt ggtggtcatc
      961 tagcacactt gaaccttttg aaacccattt gggaaaaaga agataggttt tgggtaacct
     1021 ttgataaaga agatgctagg agtattctaa gagaagagat tgtatatcat tgcttctttc
     1081 caacaaaccg taatgtcaaa aacttggtaa aaaatactat tctagctttt aaggtcctta
     1141 gaaaagaaag accagatgtt atcatatcat ctggtgccgc tgtagcagta ccattctttt
     1201 atattggtaa gttatttggt tgtaagaccg tttatataga ggttttcgac aggatagata
     1261 aaccaacttt gacaggaaaa ttagtgtatc ctgtaacaga taaatttatt gttcagtggg
     1321 aagaaatgaa aaaagtttat cctaaggcaa ttaatttagg aggaattttt taatgatttt
     1381 tgtcacagtg gggacacatg aacagcagtt caaccgtctt attaaagaag ttgatagatt
     1441 aaaagggaca ggtgctattg atcaagaagt gttcattcaa acgggttact cagacttcga
     1501 acctcagaat tgtcagtggt caaaatttct ctcatatgat gatatgaact cttacatgaa
     1561 agaagctgag attgttatca cacatggcgg cccagcgacg tttatgtcag ttatttcttt
     1621 agggaaatta ccagttgttg ttcctaggag aaagcagttt ggtgaacata tcaatgatca
     1681 tcaaatacaa tttttaaaaa aaattgccca cctgtatccc ttggcttgga ttgaagatgt
     1741 agatggactt gcggaagcgt tgaaaaggaa tatagctaca gaaaaatat
//
LOCUS       DATRQA010000220         1750 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.221, whole genome shotgun sequence.
ACCESSION   DATRQA010000220 DATRQA010000000
VERSION     DATRQA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.221"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            91..960
                     /locus_tag="VBD27_002032"
     CDS             91..960
                     /locus_tag="VBD27_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEO7025999.1"
                     /translation="MYHTTALSFLKKGYQVIPLRKDTGTPMIKFKDIQITEEVIKNTN
                     WFNCDYALLMRGIWCIDIDTHDMDEKLAKELYIMIKKMGIDLLSVLSTDKYDNGLDGY
                     SSIIRHEYKNELISNFKNTFAELTASGGMHILFKKRDDINYTQKIGVMPGVDIKANDN
                     NFVKIFPSDGREVLQAVKTLPYYDGKFEEEVFKPKQEGITTYFGCSVTYTSNGSHEGR
                     EAYERVATGTSYNRNDDLFKGACWAFENGIDIDDLTSIIGTVKGRDVFTREEFELTIE
                     SAKRKVSYVTIRT"
     gene            914..1261
                     /locus_tag="VBD27_002033"
     CDS             914..1261
                     /locus_tag="VBD27_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEO7026000.1"
                     /translation="MNQRNEKSATSLSEHDIQNLIRMELSQAGYMVFRANVGKVKTAD
                     GRFFDTGLPKGFCDLFGFKPNGQIFFIEVKNETGRIRPEQKNFMEVMASKGALAGVAR
                     SVEDALKIVNGYH"
     gene            1340..1627
                     /locus_tag="VBD27_002034"
     CDS             1340..1627
                     /locus_tag="VBD27_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HEO7026001.1"
                     /translation="MTDKINAETMQVAYNENYQTFLAKNADYGNSFEKSLNDFGFVAG
                     VVRISDKYNRLYNLISSDKNVSESLSDTLNDMANYCVMLAVWLEVKDDTKI"
BASE COUNT          630 a          245 c          376 g          499 t
ORIGIN      
        1 attttgcgtt actgcgtaac catccttctc taatgctcta tgtatcaacg ttttagaggt
       61 tacgtagtaa aaaaggaggt gcgttacctt atgtaccaca caacagcact ttcattctta
      121 aaaaaaggat atcaggttat accgcttaga aaagatactg gaacgccaat gattaagttt
      181 aaagatatcc aaataacgga agaagtgatc aaaaatacaa actggtttaa ttgtgattat
      241 gctttattaa tgcgtggcat ttggtgtatt gatattgata ctcatgacat ggacgagaag
      301 ttagctaaag agttatacat aatgataaaa aagatgggga ttgatttatt atctgtatta
      361 tcgactgata agtatgacaa tggactagat ggttattcgt caattattag gcatgagtat
      421 aaaaacgaat taatcagtaa ttttaaaaat acattcgcag aattaactgc aagtggtggt
      481 atgcacatac tctttaaaaa acgcgatgat attaattaca cgcaaaaaat aggagtaatg
      541 cctggtgttg acataaaagc gaatgataat aattttgtca aaatattccc atctgacgga
      601 cgtgaagttt tacaagcagt taaaacatta ccttattatg atggaaaatt tgaagaggaa
      661 gtatttaaac caaaacaaga aggtataaca acctattttg gatgttctgt cacatataca
      721 tcaaacggaa gtcacgaagg ccgagaggct tatgaacgtg tagcaactgg aacatcatat
      781 aacaggaatg atgatttgtt taaaggagcg tgttgggcgt ttgaaaatgg tatcgatatt
      841 gatgatttaa catcaattat tgggacggtt aaaggcagag acgtatttac gagagaggag
      901 tttgaattaa caattgaatc agcgaaacga aaagtcagct acgtcactat cagaacatga
      961 tatccaaaac cttatccgaa tggaattatc acaagcgggg tatatggtat ttcgagcaaa
     1021 tgtcggaaag gtgaaaacag cagatggtag attttttgat acaggtttac caaaaggttt
     1081 ttgtgatttg tttggattca aaccaaacgg acaaatattt tttatcgaag ttaaaaatga
     1141 aacaggtcgg ataagaccag aacagaaaaa ctttatggag gttatggcat cgaaaggggc
     1201 gttagccgga gtggcgcgtt cggtggaaga tgctttgaaa atagtaaatg gatatcatta
     1261 ataaatttaa aaagttagat cattacaagg aaattaagcg attgatgtac gcagcaatga
     1321 ctttctgagg aggtatagta tgacagataa aattaacgca gaaactatgc aagtagcata
     1381 taacgaaaac tatcaaacat ttttagccaa aaacgcagat tacggaaatt ctttcgagaa
     1441 gtctctaaac gactttggat tcgtcgctgg tgtcgtacgt ataagcgata aatacaacag
     1501 actatataat cttataagca gtgataaaaa cgtctcagaa agcctgtcag acacgttaaa
     1561 cgacatggct aattattgcg tgatgttagc ggtttggttg gaggttaagg atgataccaa
     1621 aatttagagt gtgggataaa gtaacgcaag ctatgggcgt ggttgaagca atagattacg
     1681 tcgataacaa aatatacacc ttttatcgga aagttgtccg tagatatatc cctttcgagg
     1741 aagctgtgtt
//
LOCUS       DATRQA010000221         1748 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.222, whole genome shotgun sequence.
ACCESSION   DATRQA010000221 DATRQA010000000
VERSION     DATRQA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.222"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            246..542
                     /locus_tag="VBD27_002035"
     CDS             246..542
                     /locus_tag="VBD27_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026002.1"
                     /translation="MTEKNYNNHTISLNKTEFFTITDDQVTYTKNEELEKISYDSKNN
                     ELLLNGKKFWTHLTSGELQLTDPKNTDMTLNYASKNSPLFKSYEKGTAKFKEEN"
     gene            802..1134
                     /locus_tag="VBD27_002036"
     CDS             802..1134
                     /locus_tag="VBD27_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026003.1"
                     /translation="MKNLFATTEASSRKQEQDRIVNYIKQHVELTNGNQIKKIEFIDF
                     QKNEMTGTWGISTKINEQFSISFSEDRIGGKLRALGYQPNEIGFSKDINSNNQNVNDI
                     EVIYMKKE"
     gene            1136..1645
                     /locus_tag="VBD27_002037"
     CDS             1136..1645
                     /locus_tag="VBD27_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026004.1"
                     /translation="MISEESYRYLVEDAYRVDSKKVKIPLKRSDIVGNSDYVIIEPPI
                     DNTSNGMQAMVVAPIKEGMTAEPDTSEIVIVYAGTNLGERLDIATDVEMVAGGDTYLL
                     ADPKTKTFRKSQGKSALEYAEKISSKYPNSEITTTGHSLGESEALYVALKMGWMNVGY
                     NGPDIHIMI"
BASE COUNT          712 a          199 c          313 g          524 t
ORIGIN      
        1 ataataggct gtgattgtct aaactcaatt actattcata aagattttca agaaaaataa
       61 taaataattt tggggacaaa tgatgggaca aatcctcttc tgtcaagcaa aaaagcctaa
      121 gaacaattgt aataaggaga aaagatgaaa aaaaatattt attacttctt ccaatagtat
      181 taattatagt ggtagtagtt ggcatactta atcataaaaa aatgccagat gaaggaagat
      241 attatttgac tgaaaaaaat tataataatc atactatctc attaaataaa actgaatttt
      301 ttaccataac cgatgatcaa gttacctata caaaaaatga ggagttagaa aaaatatctt
      361 atgatagcaa aaataatgaa ttgcttttaa atggtaaaaa gttttggact catctcactt
      421 ctggtgagct tcagttaaca gatcctaaga acactgatat gacattaaat tatgctagta
      481 aaaatagtcc tttattcaaa tcatatgaaa aaggaacagc aaaatttaaa gaggaaaatt
      541 aaattatatt attaactgct gaataagtta tggtgcaaga catacttgtg ggagtgttaa
      601 ggttcaagaa ggagtagtat tgaatcttca attatttggg aatctgaatt accttcttca
      661 agaaaaaaga ggtaatttaa ttaatttaaa agaagttatc tatatttgaa tggtatagaa
      721 aaaacagagg tttatttgct tatgaaaaaa cgtatatggt atttgataat aataatcaca
      781 gtaattttag gaggactagc catgaaaaac ttatttgcaa caacagaagc atcatcaagg
      841 aaacaggaac aagatagaat tgtcaattac ataaaacaac atgttgagtt aacaaatggt
      901 aatcaaataa aaaaaattga gtttatcgac tttcaaaaaa atgagatgac aggtacatgg
      961 ggaatttcta ctaaaattaa tgaacaattt tcgattagtt tttctgaaga tagaattggt
     1021 ggtaaactta gagcattagg atatcaaccg aatgaaatag gtttttcaaa ggacatcaat
     1081 agtaataatc aaaatgttaa tgatattgaa gtgatttata tgaagaaaga atagaatgat
     1141 ttcagaagaa agttacagat atttagttga ggatgcttat cgagttgatt caaaaaaagt
     1201 aaaaatacct ctgaaaagaa gtgatattgt agggaacagt gactatgtta ttattgaacc
     1261 acctatagat aatacttcaa atggtatgca agctatggta gttgctccga taaaagaagg
     1321 aatgacagct gaaccagata ctagtgaaat agtgattgtt tatgcaggta ctaatttagg
     1381 tgagcgttta gacatagcta cagatgttga aatggttgct ggaggggata catatttact
     1441 tgctgatcct aaaaccaaga cttttaggaa atctcaggga aaatcagcac tagagtacgc
     1501 agaaaaaata tcgagtaaat atcctaattc agaaattact acaaccggtc actccttagg
     1561 ggaatcagag gcactatatg tcgctttaaa aatgggttgg atgaatgttg gttataatgg
     1621 tccagatatt catatcatga tataaaatga cgaaatagat tatatgaaat ctcatccaga
     1681 ataatttaga aaaaaatcga aaaatatgag ttgtttgagt taaaatttta actatggaga
     1741 tagagtcc
//
LOCUS       DATRQA010000222         1745 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.223, whole genome shotgun sequence.
ACCESSION   DATRQA010000222 DATRQA010000000
VERSION     DATRQA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.223"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1251)
                     /locus_tag="VBD27_002038"
     CDS             complement(<1..1251)
                     /locus_tag="VBD27_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEO7026005.1"
                     /translation="MKKESISIKDILWFIIPSLFGVLLLMTPFKYNGMTTVAVSVISK
                     TINQWINAVFPIHYIILLIIFISCVLALCYRLFRPSFIEKNDLLKEISDITIFWLIIR
                     LIGLALGLMTVLHIGPEMVCGKETGGLILFDLIGGLFTIFLAAGFILPFLTEFGLLEF
                     VGVFLTPIMRPFFQLPGRSAVNCVASFVGDGTIGIALTDKQYVEGYYTSREAATISTT
                     FSAVSITFCLVVLQNVGLTDYFGHFYLTVIISSVVCAFIIPYIPPLSRQSNSYYTKPK
                     KETSEVIPDGYNKIEWGLKLAIDKSKANGNMKTFVNNASKTVLSLWFGVMPTIMTVGT
                     IALIISVSTPIFKILGTPFLPFLELLGIPEADIASQTMIVGFSDMVVPSIMAAEIHSE
                     MTRFIVATVSIVQLIYMSETGAVIL"
     gene            1483..>1745
                     /locus_tag="VBD27_002039"
     CDS             1483..>1745
                     /locus_tag="VBD27_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7026006.1"
                     /translation="MTKHKHLTLSDRNDIQSGLDRGETFKAIGLNLLKHPTTIAKEVK
                     RNKQLRESTKDCLDYPLLRKAPYVCNGCPKRRINCGYKKTFYLA"
BASE COUNT          653 a          333 c          287 g          472 t
ORIGIN      
        1 taatatcacc gcaccagtct cagacatata aattaattgc acaatagaaa ctgttgcgac
       61 aataaacctt gtcatctcac tgtgaatttc agccgccatt atcgaaggaa caaccatatc
      121 tgaaaaacca acaatcatgg tttgactcgc aatatctgct tctggaatcc ctaaaagttc
      181 aaggaacgga aggaatggtg tacctaaaat tttaaaaata ggtgttgata ctgaaataat
      241 caaagctatt gttccgacag tcataattgt tggcataacg ccaaaccata aactaagtac
      301 tgtcttagag gcattattga caaaagtctt catgtttcca tttgcttttg atttatcgat
      361 ggcaagctta agtccccact ctatcttgtt ataaccatcc ggaattacct cagatgtttc
      421 tttctttggt ttagtgtaat aactatttga ttgacgagat aagggaggga tataagggat
      481 aatgaatgca cagacaacac tgctaatgat gactgttaga taaaaatggc caaaataatc
      541 agttaagcca acattctgca aaacaacaag acaaaaagtg attgatactg ctgaaaaagt
      601 agtggaaatc gttgcagctt ctctactggt ataataaccc tcaacatact gcttatcggt
      661 caaagcaatt ccaatagtac catcacctac aaatgaggct acacaattaa ctgctgaccg
      721 accaggtagc tgaaagaatg gacgcataat gggagttagg aagacaccaa caaactccag
      781 tagaccaaat tctgtcaaaa atggaagaat aaaacccgca gcaaggaaaa tggtaaacaa
      841 accaccaatc aaatcaaaaa ggataaggcc gcctgtctct ttcccacata ccatttcagg
      901 accaatatgt aaaaccgtca tcagaccaag tgccaaaccg atcaaacgaa taatcagcca
      961 gaaaatggta atgtcagaaa tttcttttag caagtcattt ttttcaataa agctaggtct
     1021 aaacagacga taacaaagtg ctaacacaca tgaaataaaa atgattagta aaataatata
     1081 atgtatagga aaaacagcgt taatccattg attaattgtc ttagagatta cagagacagc
     1141 tactgttgtc ataccattat atttaaatgg agtcattaat aataatactc caaataacga
     1201 cggaataata aaccacaaga tatctttaat agatatactt tcttttttca tacaatcctc
     1261 ctggataata tggaattgtt tctaaatata ctcataactt ccattgtaaa attttataat
     1321 aaaaaaaaca accttgaatt aaagaaaatt ttacttagta atactttagt aatttctcaa
     1381 agtaagttcc gtctgccctc caaaaccgga agttagtgtg aaaaggattt ttatggtgga
     1441 attaagtcta agacgtttgg gttagaaatg agtttatcca tcatgacaaa acacaaacac
     1501 ctaactctct cagaccgtaa tgacatccaa tctggtttgg atagaggaga aacctttaaa
     1561 gccatcgggc ttaatctact gaaacaccct actactatcg caaaagaagt caaacgaaat
     1621 aagcaactaa gagaatcaac caaagactgc ctagactatc cgctactaag aaaagctccc
     1681 tatgtttgta atgggtgtcc aaagaggagg atcaactgtg gttacaagaa gaccttctat
     1741 cttgc
//
LOCUS       DATRQA010000223         1732 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.224, whole genome shotgun sequence.
ACCESSION   DATRQA010000223 DATRQA010000000
VERSION     DATRQA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.224"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..293
                     /locus_tag="VBD27_002040"
     CDS             <1..293
                     /locus_tag="VBD27_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO7026007.1"
                     /translation="VYEDGILLVCPECAYEWNPEEIEEEVGLIVLDSNGTRLSDGDTV
                     TVIKDLKVKGAPKDIKQGTRVKNIRLVDGDHNIDCKIDGFGAMKLKSEFIKK"
     gene            428..1069
                     /locus_tag="VBD27_002041"
     CDS             428..1069
                     /locus_tag="VBD27_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7026008.1"
                     /translation="MSYILEILPSLLVGAQVTLQVFFIVICLSIPLGIVVAFLMKVHF
                     KPLQWLLTTYIWIMRGTPLLLQLIFFYYFLPSVGISFDRMPAAIIAFTMNYAAYFAEI
                     FRGGIESIPKGQYEAAKVLKFSKFQTVWYIVLPQVFKIVLPSVFNETITLVKDSSLVY
                     ILGVGDLLLESKTAANRDATLAPMFIAGGIYLLLIGLLTILSKQVEKRFNYYK"
     gene            1079..1708
                     /locus_tag="VBD27_002042"
     CDS             1079..1708
                     /locus_tag="VBD27_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7026009.1"
                     /translation="MLELKNISKCYGQKEIFKDFNLTVEEGKILSLVGPSGGGKTTLL
                     RMLAGLEKIDSGTIVHDGKEVSVDHLETLNLLGFVFQDFQLFPHLTVLDNLILSPVKT
                     MGLSKELAKEKALVLLERLGLKDHALVYPFSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQEVEKLILQNRETGMTQIVVTHDLQFAESISDTILKINPK"
BASE COUNT          545 a          254 c          344 g          589 t
ORIGIN      
        1 atgtctatga agacggtatt ttactggttt gtccagaatg tgcttacgag tggaatccag
       61 aagaaatcga agaagaagta ggtttaattg tattggatag caatggaacg cgcttatcag
      121 atggagatac tgtgacagtt attaaggatt taaaagtaaa aggagcgcct aaggatatta
      181 agcaaggcac tcgtgttaaa aatattcgtt tagttgatgg cgatcataat atcgattgta
      241 aaattgatgg ttttggagct atgaaactaa aatctgagtt tataaaaaaa tagctttcag
      301 gcaattggcg acggtcaatt gctttttgaa tatgacttga tttcatgcat ttttattaaa
      361 aaagctctat gacattggtt tgaattttga tataatgaga acatctattt tttatttagg
      421 ggggaccatg tcttatattt tagaaatctt acctagctta ttagtaggtg cgcaagtgac
      481 attgcaagtt ttctttattg ttatttgttt atcaattcca cttggtattg ttgtagcctt
      541 cctaatgaaa gttcatttta agccgcttca atggctactg acaacttata tttggattat
      601 gcgaggcacg cctctgttat tacagcttat ctttttttat tactttttac caagtgtagg
      661 aatttctttt gatcgtatgc cagctgcgat tattgctttt acgatgaatt atgcagctta
      721 ttttgcagag atttttcgag gaggtattga gtctattcct aaagggcaat atgaagctgc
      781 aaaggtatta aagttttcta agtttcaaac ggtttggtac atagtattac ctcaagtctt
      841 caaaatagtc ttaccgagtg ttttcaatga aacgattact ctagtgaaag attcctcatt
      901 ggtttacata cttggtgtgg gcgacttact tctagaaagt aagacagccg caaataggga
      961 tgcaacactt gcaccaatgt ttatagcagg tggtatttac ctattattaa ttggtctttt
     1021 aactatttta tctaaacaag ttgaaaagcg ttttaactac tataaataag gagggacgat
     1081 gttagaatta aaaaatattt ctaaatgcta tgggcaaaaa gaaatcttta aagatttcaa
     1141 tctgactgta gaagaaggta aaattctctc tttagtagga ccttctggag gtggaaaaac
     1201 aacactatta cgtatgttag ctggattgga aaagattgat tcagggacta ttgttcacga
     1261 tggtaaagaa gtatctgttg atcatttgga aacactaaat cttttaggat tcgtcttcca
     1321 agactttcaa ttattcccac atttaacagt attagataat ttgatcctct ctcctgttaa
     1381 aacgatggga ttatcaaaag aacttgctaa agaaaaagcc ttagtattac ttgaacgctt
     1441 gggacttaaa gaccatgctt tagtctatcc attttcatta tctggggggc aaaagcaacg
     1501 tgtcgcacta gctcgtgcga tgatgattga tccacagatt attggttatg atgagccaac
     1561 tagcgctctt gatccagagt tgcgtcaaga agtagaaaaa ctaattttac aaaatagaga
     1621 aacaggtatg acacaaattg tagtaacaca tgatcttcaa tttgctgaaa gtatatctga
     1681 tacgattctc aaaattaatc ctaagtagac ttgggggtag attgttgact ca
//
LOCUS       DATRQA010000224         1729 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.225, whole genome shotgun sequence.
ACCESSION   DATRQA010000224 DATRQA010000000
VERSION     DATRQA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.225"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            53..1255
                     /locus_tag="VBD27_002043"
     CDS             53..1255
                     /locus_tag="VBD27_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7026010.1"
                     /translation="MEDKLFNKHFIGITILNFIVYMVYYLFTVIIAFIATKELGVSTS
                     QAGLATGIYIVGTLIARLIFGKQLEVLGRKLVLRGGAIFYLLTTLAYFYMPSIGVMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPADKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HINFKMVIVLCSILIAIVVLGAFVFPVKNITLNPEQLAKSKSWTIDSFIEKKAIFITI
                     IAFLMGISYASVLGFQKLYTTEINLMTVGAYFFIVYALVITLTRPSMGRLMDAKGDKW
                     VLYPSYLFLTLGLALLGSAMGSVTYLLSGALIGFGYGTFMSCGQAASIKGVEEHRFNT
                     AMSTYMIGLDLGLGAGPYILGLVKDGFLGAGVQSFRELFWIAAIIPVVCGILYFLKSS
                     RQVETKTI"
     gene            1273..1725
                     /locus_tag="VBD27_002044"
     CDS             1273..1725
                     /locus_tag="VBD27_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO7026011.1"
                     /translation="MTQKYERILIAIDGSYESELAVEKGINVALRNDAELLLTHVIDA
                     HAYQSEGVFSDYVFDRQEQESADVLAYFEKLAHSKGLTKIKKITEIGNPKTLLAKDIP
                     IREKADLIMVGATGLNTFESLLIGSTSEYILRHSKVDMLVVRDSKKTL"
BASE COUNT          527 a          250 c          351 g          601 t
ORIGIN      
        1 tatttttgta aaatgtttat gaaaatgaaa ttgaaaaaag gagccatttg cattggaaga
       61 caaattattc aacaaacatt ttataggcat tactatttta aactttattg tttatatggt
      121 ctattatttg tttaccgtta tcatagcttt tattgcgact aaagagttag gtgttagcac
      181 tagccaagca ggattagcaa cggggattta tattgtaggg actttgattg ctcgtcttat
      241 atttggtaag caattagaag ttctaggacg taagttagtt ttacgtggag gggctatttt
      301 ttacttacta acaactttag cttattttta tatgccaagt atcggagtaa tgtatttagt
      361 tcgtttccta aatggttttg gttatggcgt cgtgtcaaca gcaactaata ctattgtaac
      421 agcctatata ccagctgata aaagaggtga ggggattaac ttttacggtc tatcaacaag
      481 tttagccgca gctattggtc cttttgtagg aacatttatg ctagacaacc ttcatattaa
      541 ctttaaaatg gttattgtat tatgtagtat tttaattgcg attgtagtgt tgggagcatt
      601 tgttttccca gtcaaaaata ttactttaaa tccagaacag ttagctaaat caaaatcatg
      661 gactattgat agtttcattg agaaaaaagc aatttttatc acaattattg catttttgat
      721 gggtatctcc tatgcttccg tgttaggttt ccaaaaatta tatacaacag aaattaattt
      781 gatgacagta ggagcttatt tctttattgt ttatgcactt gtcatcactt taaccagacc
      841 atctatggga agattaatgg acgctaaggg agataagtgg gtgctttatc caagttatct
      901 gttcttaact ttgggacttg ctttattagg gagtgctatg ggaagtgtta cctaccttct
      961 atcaggtgct ttgattggtt ttggttatgg cacctttatg tcttgtggcc aagcagcatc
     1021 aatcaaaggt gttgaggaac atcgtttcaa tacagccatg tcaacttaca tgataggtct
     1081 tgatttaggg ttaggtgctg gaccttacat tttgggactt gttaaagatg gttttcttgg
     1141 agctggtgtg caatccttta gagaattatt ctggatagca gcgattattc ctgttgtttg
     1201 tggtattcta tatttcttaa aatcatctag acaagttgaa actaaaacta tataaaaggg
     1261 aggaattaag ctatgacaca aaaatatgag cgtattctta ttgctattga tgggtcttac
     1321 gagtcagaat tggcggtaga aaaagggata aatgttgctc ttcgtaatga tgctgaatta
     1381 ctgctaacgc atgttataga tgctcacgct taccaaagtg agggagtctt tagtgactat
     1441 gtttttgata ggcaagaaca agagtcagca gatgtgctag cctattttga gaaattagca
     1501 cattcaaaag gccttacaaa gattaaaaaa attactgaaa tcggaaatcc taagactctt
     1561 ttggcaaagg atatccccat aagggaaaaa gcagatttaa ttatggtagg cgcaacagga
     1621 ttaaacactt ttgaaagcct attgatcggc tcaacttcag aatacatttt acgtcattcg
     1681 aaagttgata tgctagtcgt tcgtgatagt aagaaaacct tataaacta
//
LOCUS       DATRQA010000225         1676 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.226, whole genome shotgun sequence.
ACCESSION   DATRQA010000225 DATRQA010000000
VERSION     DATRQA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.226"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(86..1648)
                     /gene="guaA"
                     /locus_tag="VBD27_002045"
     CDS             complement(86..1648)
                     /gene="guaA"
                     /locus_tag="VBD27_002045"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO7026012.1"
                     /translation="MTDISILNDIQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TADEIRDINPIGIVLSGGPNSVYADGAFGIDEEIFELGIPILGICYGMQLITHKLGGK
                     VLPAGEAGHREYGQSALRLRSESALFAGTPQEQLVLMSHGDAVTEIPEGFHLVGDSVD
                     CPFAAMENTEKQFYGIQFHPEVRHSVYGNDILKNFAVNICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMDMLG
                     GKFGLNIIRVDASKRFLDLLSGVEDPERKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTALGMPDEV
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
BASE COUNT          540 a          355 c          297 g          484 t
ORIGIN      
        1 acataaaaat gatattttaa gtgtaagtat cccttattca agaaataaga gtaaaaaaac
       61 ttagctactt aaagtagcta agtttttatt cccactcaac agttgcaggt ggtttacttg
      121 taatatcata aacaatacgg ttaacgtgat caacttcgtt aacaatacgt gtcgaaattt
      181 tcttaagtac atcccaagga agttgcgcaa agtcagctgt catgccatca attgaagtaa
      241 ttgcacggat agcaattgta tagtcataag tacgaccatc tcccataaca ccgacagaac
      301 gtacgccagt attaactgtg aaatactgcc atacgtcacg atctagacca gctttagcga
      361 tttcttcacg aagaatagca tctgattcgc ggactgtttc aagtttttct tcggtaattt
      421 cacccataac gcgaatagca agaccaggcc cagggaatgg ttgacgccat acaacttcat
      481 caggcattcc aagtgcagta ccaagagcgc gaacctcatc tttgaataat gtgttaagtg
      541 gctcaatcaa ttcaaattgc atatcttctg gcaatccacc aacattatgg tgtgacttga
      601 ttgtttgggc tgtttctgtc ccagattcaa taatatctgt gtagagggta ccttgagcaa
      661 ggaaatcaac gcctttaagt ttacttgcct catcatcaaa aacgtacacg aactcatttc
      721 cgatgatttt tcgttttctc tcgggatctt caacaccaga aagaagatct aggaagcgtt
      781 ttgaagcatc aacacggata atattaagtc caaatttacc accaagcata tccataactt
      841 ggtcaccttc atttttacgt aataaaccat gatctacaaa aatacatgtt aattgatcac
      901 cgatagcacg ttgtaggagt actccgacaa cagatgaatc aacaccgcca gatagtccaa
      961 gaagtacttt acgatcgcct acagtttcac gtatttttgc aatttccata tcaatgaagt
     1021 tatccattga ccaatcacca cgtgcaccac agatattaac agcaaaattt ttaaggatat
     1081 cattgccata tactgagtga cgaacttcgg ggtggaattg gataccataa aattgttttt
     1141 cagtattttc cattgcagca aatggacagt caacagagtc accaacgaga tggaaaccct
     1201 cagggatttc agtaacagca tcaccatggc tcatcaaaac aagttgttct tgaggagtgc
     1261 cagcaaagag tgctgattct gagcgtaggc gaagtgctga ttgaccatac tcacggtgac
     1321 cagcttctcc cgcaggaaga accttaccac cgagtttatg ggtaatcagt tgcataccat
     1381 aacaaattcc taagattgga attcctaatt caaaaatttc ttcatcgatt ccaaaagcgc
     1441 catcagcgta tacggaattt ggaccacctg aaagtacgat accaatagga ttaatatcgc
     1501 gaatttcatc agccgtaatt ttatgtgatt ttaactcaga aaatacacca aattcacgga
     1561 tacgacgtgc gataagctgg ttatattgac taccgtaatc tagaacgata attttttgga
     1621 tatcattcaa tatagaaata tcagtcattg tttccctttc tttgtagcta atttta
//
LOCUS       DATRQA010000226         1545 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.227, whole genome shotgun sequence.
ACCESSION   DATRQA010000226 DATRQA010000000
VERSION     DATRQA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.227"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(71..805)
                     /locus_tag="VBD27_002046"
     CDS             complement(71..805)
                     /locus_tag="VBD27_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7026013.1"
                     /translation="MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGIDITDKKNDIFKMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGLSKLDAQTKAYELLEKVGLKEKANAYPASLSGGQQQRIAIARGLAMNPD
                     VLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGII
                     VEQGTPKKVFEQTKEIRTRDFLSKVL"
     gene            complement(798..1490)
                     /locus_tag="VBD27_002047"
     CDS             complement(798..1490)
                     /locus_tag="VBD27_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7026014.1"
                     /translation="MNFSFLPQYWSYFNYGVMVTIMISTCVVFFGTIIGVLIALVKRT
                     NLHFLTILANFYVWVFRGTPMVVQIMIAFAWMHFNNLPTISFGVLDLDFTRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPSGQIEAAYSLGIRPKNTLRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPVAPLLFAAFYYLMLTTILSALLKPM
                     EKYLGKGVKIDG"
BASE COUNT          535 a          301 c          235 g          474 t
ORIGIN      
        1 aatacagtaa caatttactc ataacaatgg aataataaaa gtctaaaaca agttgtttta
       61 gacaatgaaa ttataatact ttacttaaga agtctcttgt gcggatttct tttgtctgct
      121 caaatacttt cttaggggtc ccttgctcaa caataatccc tgcatccata aaaatgacac
      181 gatccgctac ttcacgtgca aaacccattt catgagtgac aataaccatc gtcataccag
      241 atttagctaa atcttgcata acagtcaaga cttcacctac catttcagga tcaagagctg
      301 aagtaggttc atcaaaaaga aggacatcag gattcattgc aagacctctt gcaatagcaa
      361 tccgttgttg ttgtcctcca gataagcttg ctggataagc attagccttc tctttgagtc
      421 caactttttc aagtagctcg tatgcttttg tctgagcatc aagcttagaa agtccctttg
      481 tcttaatagg tgataaagta atattttcta gtacagtcat attgggaaat agattgaact
      541 gttgaaaaac catgcccatt ttttcgcgca ttttaaaaat atcattcttt ttgtctgtta
      601 tatcaatccc ttcaaaagtc actgttccct ttgttggtac ttccaagaga ttcattgttc
      661 ttaaaaatgt tgacttacca gagccagaag ggccaataat aaccaccact tctccttgat
      721 gaatatccaa gtcaatgcct tttaaaacct catttttccc aaaactttta tgaagtcctt
      781 gtattgaaat aattggctca accatctatt tttacccctt tcccaagata tttctccatt
      841 ggttttaaca aagctgagag aatcgttgtc aacattaaat agtaaaatgc tgcaaataat
      901 aacggtgcaa ctggtgagta agtagccgtt acaactgatt gtgctccgtt ccataattcc
      961 atgacaccaa tagtttgaag gagagcacta tctttaataa ttgtaataaa ttcattccct
     1021 agagcaggta aaatattttt aaaagcttgg ggtaagataa catagcgaag tgtattttta
     1081 ggtcgaatcc ccaacgagta agctgcttct atttgtccag atggtacagc ctcaatccct
     1141 gcacgtacaa tttccgaaat ataggcacca ctatttaagg aaatgataat gataccaggt
     1201 aaaagtcgtg taaaatctaa atctaaaaca ccaaagctaa ttgttggtaa attgttaaaa
     1261 tgcatccatg cgaaagcaat cataatttga actaccatcg gtgtcccacg aaatacccat
     1321 acatagaaat tagctaatat tgtgagaaaa tgtaaattag tacgctttac taaagcaatt
     1381 aacacgccta taatagttcc aaaaaaaaca acacatgttg aaatcataat ggttaccatc
     1441 acaccataat taaaatagga ccaatattgt ggtaaaaatg aaaaattcat tgacatctcc
     1501 taaaaaagta attgccaata gtctatcaca caaagaataa aaata
//
LOCUS       DATRQA010000227         1544 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.228, whole genome shotgun sequence.
ACCESSION   DATRQA010000227 DATRQA010000000
VERSION     DATRQA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.228"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..325
                     /locus_tag="VBD27_002048"
     CDS             <1..325
                     /locus_tag="VBD27_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026015.1"
                     /translation="PIPSELTFIKFYGHGLGEADYSYFQSIFDSINLYSSDTVLIFYW
                     SCYNENIREQIKSQQVHAVHKLIEKYGDTFNNKNHGRNLFTKLQLENRLIIQEILYND
                     ILNPY"
     gene            complement(460..825)
                     /gene="rplL"
                     /locus_tag="VBD27_002049"
     CDS             complement(460..825)
                     /gene="rplL"
                     /locus_tag="VBD27_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO7026016.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGEAAAAKDSFDVELTAAGDKKVGVIKVVREITGEGLKEAKAIVDNAPSVIKEGASEA
                     EANEIKEKLEAAGASVTLK"
     gene            complement(889..1389)
                     /locus_tag="VBD27_002050"
     CDS             complement(889..1389)
                     /locus_tag="VBD27_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO7026017.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVEQDTNLRR
                     SLRESDVEFKVIKNSILTRAAEKAGLEDLKELFVGPSAVAFSNEDVIAPAKVISDFAK
                     DAEALEIKGGSVDGKFTSVEEINALAKLPNKEGMLSMLLSVLQAPVRNIAYAVKAVAE
                     KDEEVA"
     misc_feature    complement(1419..>1544)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT          472 a          296 c          246 g          530 t
ORIGIN      
        1 tccaattccc tctgaactaa cttttataaa attttatggt catggtctgg gtgaagcaga
       61 ctattcttat tttcaatcaa tttttgactc aattaatctt tattccagtg acactgtact
      121 aattttctat tggagttgtt ataacgagaa tattagggaa caaattaaat ctcaacaagt
      181 tcatgcagta cataaattaa ttgaaaaata tggagatact tttaataata agaatcatgg
      241 cagaaattta ttcacaaaac ttcaattaga aaatcgttta attattcaag aaatacttta
      301 taatgatatt ctaaatccat attaaacaaa acacccccac aaaatattgt gggggtgttt
      361 gtggggaaga ttcaattgaa tctttccttt tttaatcgcc ttaactttaa cgaaaagact
      421 ttaaaactag gctttcacct ttaaacaacc aatagatgat tatttaagag taactgaagc
      481 acctgcagct tcaagttttt ctttgatttc gttagcttct gcttctgaag caccttcttt
      541 gataactgat ggtgcgttat caacgatagc tttagcttct ttaagacctt cgcctgtgat
      601 ttcacgaaca actttgataa cgccaacttt tttgtctcca gcagctgtta attcaacgtc
      661 aaatgagtct ttagcagcag cagcttcacc accagcagcg gcagcagcta caggagcagc
      721 agcagttaca ccaaattctt cttcgatagc ttttacaaga tcgttaagtt caagaattgt
      781 agcttcttta atttcagcaa taatattttc aatgtttaat gccattgtta tttcctccaa
      841 aaatatgttt taatataaaa ttatgtagct aggctacttg aagagtgctt atgccacttc
      901 ttcgtctttt tctgcaactg ctttgacagc atatgcaatg ttgcgtactg gcgcttgaag
      961 tactgaaagg agcattgaaa gcataccttc tttgtttggt aatttagcaa gagcgttaat
     1021 ttcttcaact gaagtgaatt taccgtctac tgaaccacct ttgatttcaa gagcttctgc
     1081 atctttagcg aaatcgctaa taactttagc tggtgcaata acatcttcgt tagaaaacgc
     1141 aactgctgat ggaccaacaa aaagttcttt aaggtcttca agacctgctt tttcagctgc
     1201 acgagttaaa attgagtttt tgataacttt aaactcaaca tcgctttcac gaagtgaacg
     1261 acgtaagtta gtatcttgct caacagtaag accacgtgaa tcaacaacaa cgatacttgc
     1321 tgctgctttc attttctcag caataagttc aacttgttca gctttttttg caataattgc
     1381 ttcactcatt agttttacct ccgtttttat tttttgggat aggcacaaaa aaatcgcacc
     1441 taaacctaga cacgaaagta caatacgtta ttttataatg acgttttgtg cctcggtagg
     1501 aatattatga gcactgctcc cctactgtct taggtcagtt tcat
//
LOCUS       DATRQA010000228         1534 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.229, whole genome shotgun sequence.
ACCESSION   DATRQA010000228 DATRQA010000000
VERSION     DATRQA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.229"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(227..415)
                     /locus_tag="VBD27_002051"
     CDS             complement(227..415)
                     /locus_tag="VBD27_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, toxin component, MazF
                     family protein"
                     /protein_id="HEO7026018.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFNDEVIKTLRFTT
                     KYENKSDNIRSQYLEVID"
     gene            complement(412..705)
                     /locus_tag="VBD27_002052"
     CDS             complement(412..705)
                     /locus_tag="VBD27_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO7026019.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNKSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEYFK"
     gene            762..911
                     /locus_tag="VBD27_002053"
                     /pseudo
     CDS             762..911
                     /locus_tag="VBD27_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1417..>1534)
                     /locus_tag="VBD27_002054"
     CDS             complement(1417..>1534)
                     /locus_tag="VBD27_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001920755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7026020.1"
                     /translation="KGNKLPATGENATPFFNVVALTIMSSVGLLSVSKKKED"
BASE COUNT          554 a          280 c          175 g          525 t
ORIGIN      
        1 gaattagtcg aacaagcaac taaaaatact gtagatatag ctaaaatcat caatcctaat
       61 aatttctttt tcataactaa acttcctttg ctatgagaaa atcttttaac ctctcaacat
      121 ctctatcgct taacttacct ataattttaa tattgaaccc tttattctca acatcataat
      181 attgaatagt atctatccaa gaacgcctat ttaaatttgc ttttgcctaa tcaataactt
      241 ctaaatactg acttcttata ttgtcagact tattttcata tttagtagta aatcttaaag
      301 tttttattac ttcatcatta aatttgacta ctacagcagg acgaaccttt gaacctgtac
      361 catcagaata ttcaatacga gaaactaata cagaatatac ctcaatatca ctcatttaaa
      421 atattcctca tattctgaaa aatcttcatc tccaaaaaac gcatttacac tttctggatc
      481 actagcctta actcgtattc cctttaattt tgtttctacc aaattttgaa attcttcatc
      541 agaaacagat gatttatttt ttatttcaaa aggaatactt tgagttttaa cagattgaat
      601 caaaaacatt tttactgctg tgctcatatc caaccctaag gattgaaaaa gttctgtagc
      661 atcatctttt aattgatcat caactcttac tgctactgtt gacataatat cacctcaaat
      721 catttttgta taactattgt atcactaaat gcaatacaac gcaaacaaaa aaggaaatcg
      781 tctttcccaa tgaagaggct ctagaatact atctagtcac actatttaat gattacaact
      841 tcaagcaaga acaacgaatc cacaaaggtt ttggccaatg tacagacaca cttgaaagcc
      901 tatttgatta acaatcccag ctttgccagt gtgtttacac aacattattg acacactcct
      961 tttattttta taaattaccc ttaaatacat ttaattactt gaaaagacaa acagtcattt
     1021 tagaaaattt tgcatctaac cttacatttg attacaccta aaaattttaa atcatcaaaa
     1081 gagagcctag gattaacatc ttaggctcta atgatacact tcatttacaa atttcaacta
     1141 aataggatca tttcttcagt tttatagtaa gcgtccaata acaaggtata ttggatgctt
     1201 acggaatagg gaagaaaatg gaagatacct cgttattgga cgcttacaca aaatcaaaat
     1261 cctatcgaaa ttaataatct cgataggatt tttcaagaat taaactcgaa aaattgaaac
     1321 actttaatta agtttgttga agagagatac ataactttat taatgtgttc acaccaataa
     1381 atggtgaaaa gtttagtgac attttaggtc aaaagattaa tcctcttttt tcttagaaac
     1441 agataataaa ccaactgatg acataattgt caaagctaca acattaaaga atggagttgc
     1501 attctcacct gttgctggta gtttatttcc ttta
//
LOCUS       DATRQA010000229         1526 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.230, whole genome shotgun sequence.
ACCESSION   DATRQA010000229 DATRQA010000000
VERSION     DATRQA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.230"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            46..1491
                     /locus_tag="VBD27_002055"
     CDS             46..1491
                     /locus_tag="VBD27_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO7026021.1"
                     /translation="MKKTIGIIGMGVSGLAVLLALSRKKEKELKQLAIFCFDDTVHFG
                     RGIPFQEDVDSALINSPINDISFDYRHMSDFMDWLEENYYDTNLTYVSRTLYGQYLSE
                     RTKLLLDKLPVTVIKTRVDKLNYLSDEQQWQVYIQDKLFPVTFDELHLACGALPVIEP
                     YHLEGKTDYIKDPYPIKYLAQENWKGKTVAIIGTGLAAIDVIKWLLLNTQSSVKAFSR
                     TNYFPSVRIVNDLPLTWHFFTNDNLKAFLSHPSKTFTIADFNDLMSGELEHLGFKNWK
                     NTTETFLSPGIDGLKLAFKYPKQLYLLQQLASRITVWLTDLWPLMSLSDKKEYKALYG
                     KAIINLRNPMPDESAKVILKAAQKGTFSLINNVKTITKNDEGFSIEKEKQNHISVDRV
                     INATGYQLNTKNWTKANPLLKSLINQEFCQIDDQGGITILTENAQVLSPKYGPMPGLY
                     AHGALVNGVVYQNNSTIKIQQMAERAFSDNK"
BASE COUNT          518 a          279 c          250 g          479 t
ORIGIN      
        1 ttaaatcaaa atttatgcta tgatagtggt aaggaggctt gttctgtgaa aaaaacaatt
       61 ggtattatag gtatgggagt tagtggttta gctgttctac tagcactttc tcgtaaaaaa
      121 gagaaagagt taaaacagtt agctattttt tgttttgatg atactgtcca ctttggacga
      181 ggaattcctt ttcaagaaga cgttgactca gctttaatca attccccaat caacgatatt
      241 tcatttgatt atcgccatat gtctgacttc atggattggt tagaagaaaa ttactatgac
      301 acaaatctaa cctatgtatc aaggacactt tacggacaat acctttcaga aagaactaag
      361 ttacttttag ataaactccc tgtcacggtt attaaaacaa gagtggataa actaaactat
      421 ttatcagatg agcaacaatg gcaagtctat attcaagata agctttttcc tgtcacattt
      481 gatgaattac acttagcttg tggtgctctg cctgttatag aaccttatca tttggaaggg
      541 aagaccgatt atatcaaaga cccttatcct atcaaatact tggctcagga aaattggaag
      601 ggaaaaacag ttgctatcat cgggactggt ctagctgcaa ttgatgttat aaaatggttg
      661 ctactcaata cacaatcttc tgttaaagca ttttcaagaa ccaactactt ccctagcgtc
      721 cgtattgtta atgatttacc acttacttgg cactttttta ccaatgataa ccttaaagca
      781 tttctatcac atccttcaaa aaccttcact attgccgatt ttaatgattt gatgtctggc
      841 gaattggagc atcttggctt taaaaactgg aaaaatacaa cagaaacttt tttatcccca
      901 ggaattgatg gtctcaagtt agcctttaaa taccctaagc aactctatct attacaacaa
      961 ttagcttcac gaatcactgt ctggttaact gacctttggc cactcatgtc attatcagat
     1021 aagaaagagt ataaagcact ctatggaaaa gcgattatta acttgcgaaa tcctatgcct
     1081 gatgaatctg ctaaagtaat tttgaaagct gcacaaaaag ggactttttc tctgatcaat
     1141 aacgtcaaga caattacaaa aaatgacgaa ggattttcaa tagaaaaaga aaaacaaaat
     1201 catatatctg tcgatcgcgt cattaacgct actggttacc agttaaacac taaaaactgg
     1261 acaaaagcca atccacttct aaaatcgctc atcaatcaag aattttgtca aattgacgat
     1321 caaggaggga ttactatttt aactgaaaat gcacaagttt tgtctccaaa atatggtcca
     1381 atgccaggtt tatatgcgca tggtgcactg gttaatggtg tcgtttatca gaataactct
     1441 acaattaaaa ttcaacaaat ggcagagcga gccttttctg ataataaata accatttcta
     1501 aaaaatacct cctcttttat tattta
//
LOCUS       DATRQA010000230         1483 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.231, whole genome shotgun sequence.
ACCESSION   DATRQA010000230 DATRQA010000000
VERSION     DATRQA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.231"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..472)
                     /locus_tag="VBD27_002056"
     CDS             complement(<1..472)
                     /locus_tag="VBD27_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7026022.1"
                     /translation="MHSTIYKCHHCRYLIYKSFKDIFPFALIFSWIEILQKLVISDDS
                     FLAQTTKSSIYKNEWLYTVSNTLYDLSHLLIYFMASYFVASLVWRGLSNQTKESFSAS
                     LLGFATVWLLLTSVVPGTDYHYPSQPIWLMLALISYLFIFLTRLLQNRHHIFQYS"
     gene            808..>1483
                     /locus_tag="VBD27_002057"
     CDS             808..>1483
                     /locus_tag="VBD27_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO7026023.1"
                     /translation="MSKKSHRQYQIYEGLRCAVALCFISGYINAFTYVTQGKRFAGVQ
                     TGNLLSFAIHLSNKHYSQALAFLLPIMVFMLGQSFTYFMNRWANKHQLHWYLLSSFAL
                     TQVAIVTIILTPFLPSSFTVAGLAFFASIQVDTFKSLRGAPYANMMMTGNIKNAAYLL
                     TKGLYEKNSDIFLIARNTIIIIGGFIFGVVCSTYFSSKLGEWSLSLILIPLLYVNLLL
                     GHEFYNL"
BASE COUNT          501 a          219 c          258 g          505 t
ORIGIN      
        1 tactatactg gaaaatatga tgtcgatttt gaagcagtcg agttaaaaag ataaaaagat
       61 acgaaatcaa agccagcata agccaaatgg gttggctagg atagtgataa tctgtacctg
      121 gaacaactga agttagaagc aaccaaacag tggcaaagcc aagtaaactt gcagaaaaac
      181 tttctttagt ttggttagat aagcctctcc atactaaact agccacaaaa taactagcca
      241 taaagtagat tagtaaatga gataaatcat aaagagtatt ggaaaccgta tataaccact
      301 cgtttttata aatactggac tttgttgttt gagctaaaaa agaatcatct gatataacga
      361 gtttttgaag gatttcaatc caagaaaaaa tgagagcaaa aggaaagata tctttaaaac
      421 ttttataaat tagataacgg caatgatgac atttataaat agtgctgtgc ataatctgta
      481 ctgacctcct aataggttgt atttattgta acaaatgtta agaatggtgc aagttttttg
      541 gtagtaaaat taaaagtaac taattaaact tcaaattaag ggtaaatgac ttatgagatt
      601 ttctttatcc tttcataaag tgagattgct tcaatgctga ggctatgata tactaaaact
      661 acatttatca ttgcttaata atttatttag aactgttagg ctagctagtg ctaatattaa
      721 tactatttta aaccaatcta ttagaaaaaa agtgctgagc aagattgccc agtcttattt
      781 ttgtttgcca taaagttaga ggaaaatatg tctaaaaagt ctcaccgtca atatcaaata
      841 tatgaagggc tcaggtgtgc ggttgcactc tgttttattt caggttatat caatgctttt
      901 acttatgtaa cacaaggaaa acgttttgca ggtgtacaaa cagggaattt gttatctttt
      961 gctattcatc ttagtaataa gcattatagt caagcgcttg cttttttgct acctataatg
     1021 gttttcatgt taggtcaatc gtttacttat ttcatgaatc gttgggcgaa taaacatcaa
     1081 ctgcactggt acctattatc tagttttgcg ttgacacagg ttgctatagt aacaattata
     1141 ttgactcctt tcttaccatc gtcgtttact gtggcaggac tagccttctt tgcttcaata
     1201 caagtagata cctttaaatc gctcagagga gcaccttatg ctaatatgat gatgactggt
     1261 aatatcaaga atgctgctta tttattaaca aagggacttt acgaaaaaaa ttctgatatt
     1321 ttcttaattg ctagaaatac gattattatt attggaggat ttatctttgg tgttgtttgc
     1381 tcgacttatt tttcaagtaa actgggcgaa tggagcttat cattaatatt aattcctctt
     1441 ttatatgtca acttgctgtt aggccatgaa ttttataacc tac
//
LOCUS       DATRQA010000231         1479 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.232, whole genome shotgun sequence.
ACCESSION   DATRQA010000231 DATRQA010000000
VERSION     DATRQA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.232"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            74..1429
                     /gene="rlmD"
                     /locus_tag="VBD27_002058"
     CDS             74..1429
                     /gene="rlmD"
                     /locus_tag="VBD27_002058"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO7026024.1"
                     /translation="MNVVLKQRIPLKIKRMGINGEGIGFYKKTLIFVPGALKGEEVFC
                     QISSVRRNFAEAKLLKINKKSKNRVDPACSIYKECGGCQIMHLQYDKQLEFKTDIIRQ
                     ALMKFKPEGYENYEIRKTIGMSEPEHYRAKLQFQVRSFGGNVKAGLYAQGTHRLIDIK
                     DCLVQDRLTQEMINRVAELLGKYKLPIYNERKIAGVRTVMIRRAQASGEVQLIFITSK
                     RLDFDDVVIELVREFPELKTVAVNINASKTSDIYGQITEVIWGQESINEEVLDYGFSL
                     SPRAFYQLNPKQTQILYSEAVKALDVKEDDDLIDAYCGVGTIGLAFAGKVKSVRGMDI
                     IPEAIQDAKENALHMGFTNTHYEAGKAEDIIPRWYSEGFRANALIVDPPRTGLDDKLL
                     NTILKMPPEKMVYVSCNTSTLARDLVTLTKVYHVHYIQSVDMFPHTARTEAVVKLQRK
                     V"
BASE COUNT          503 a          200 c          312 g          464 t
ORIGIN      
        1 aaatagtaaa aatgagtctt tttttaaatg ttatggattt atagtatttt cttagttagg
       61 gagtaatgaa ctgatgaatg tggttttaaa acagcgaatt cctttaaaaa ttaaacgaat
      121 gggaattaat ggtgaaggaa taggttttta taaaaaaaca ctaatctttg ttccaggggc
      181 tctcaaggga gaagaggtgt tttgtcagat ttcttctgtt agacgtaatt ttgcagaagc
      241 taaactgtta aagataaata aaaagtctaa aaatagggta gatcccgctt gttctattta
      301 taaagaatgt gggggatgcc aaataatgca ccttcaatat gataagcagc tagagtttaa
      361 aacggatatt attcgacagg ctctaatgaa atttaaacca gagggatatg agaattatga
      421 aatccgtaaa acgataggta tgtcggaacc tgaacactat cgtgctaaat tacaatttca
      481 agtgcgttcg tttggaggga atgtaaaggc aggtttatat gcacaaggga cacatcgatt
      541 gattgatatc aaagattgtt tagtgcaaga taggcttact caggaaatga taaatcgagt
      601 tgcagaactt ttaggaaaat ataaattgcc tatttataat gaacgtaaaa tagctggtgt
      661 tcgtactgtt atgattcgac gagcacaagc gagtggagag gttcaactga tttttatcac
      721 aagtaaaaga cttgattttg atgatgttgt tattgaatta gttcgagagt ttcctgagtt
      781 aaagacagtt gctgtaaata tcaacgctag taaaacaagt gatatctatg gtcaaataac
      841 agaagttatt tggggtcaag aatccatcaa tgaagaagta ttagattatg gtttttcgtt
      901 atcaccacgt gctttttatc aacttaatcc taaacaaaca caaattttat atagtgaggc
      961 agtcaaggcc ttagatgtga aggaagatga tgatttaatt gacgcttatt gtggagtagg
     1021 aacgattgga cttgcttttg ctggaaaagt taaaagtgtt cgtggaatgg atattatccc
     1081 tgaggctatt caggatgcta aagagaacgc acttcatatg ggttttacta atacacatta
     1141 cgaagctgga aaggcagagg atattatccc taggtggtat agtgagggat ttcgcgctaa
     1201 tgcgttaatc gtggatcctc ctcgcacagg cttagatgat aaattgctaa atactatact
     1261 aaaaatgcct ccagaaaaaa tggtttacgt ttcttgtaat acatctacgc ttgctagaga
     1321 cttagtaaca ttaacaaaag tataccatgt tcactacatc caatctgttg acatgtttcc
     1381 acatacagca aggaccgagg ctgtagtgaa actacaacgt aaggtataat aaagttttct
     1441 aatctaaaaa taagagtatt tttgggtatt cttattttt
//
LOCUS       DATRQA010000232         1453 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.233, whole genome shotgun sequence.
ACCESSION   DATRQA010000232 DATRQA010000000
VERSION     DATRQA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.233"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            28..504
                     /locus_tag="VBD27_002059"
     CDS             28..504
                     /locus_tag="VBD27_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026025.1"
                     /translation="MYIIPAGYSPERLDSEVLTAKIEDDEALLRKIQDLKDHLDKNTK
                     ILAYAFDGLEVRVKARLRKNRKKLKNLIEFNAKSTSVFNELKDYSNAVSSGLTFVSKG
                     FSSFNGQFSSASNLDWQKTIKTGMRKREEDAKQLRKKALEDSNKLRKIASKRGYFE"
     gene            760..1097
                     /locus_tag="VBD27_002060"
                     /pseudo
     CDS             760..1097
                     /locus_tag="VBD27_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          542 a          183 c          276 g          452 t
ORIGIN      
        1 attgactgag ttactctttt ttaaaaaatg tatatcattc cagctggtta ctcacctgaa
       61 cgtttagact cagaagtcct tacagcaaag attgaagatg atgaagcctt attgcggaaa
      121 attcaagacc ttaaagatca tttagataaa aataccaaaa tcttggctta cgcttttgat
      181 ggcttggaag tcagagtgaa agctagacta agaaaaaatc gtaagaaact caaaaacctt
      241 atagaattca atgccaaatc tacctcagtc tttaatgaat tgaaggatta ttcaaatgct
      301 gtatcgtctg gtttaacctt tgtatctaaa ggctttagta gtttcaatgg ccagttctct
      361 tcagcctcaa atcttgattg gcaaaagact attaagacag gaatgagaaa acgagaagaa
      421 gatgctaaac agttgagaaa aaaagcttta gaagattcaa ataagctcag aaaaattgca
      481 tcaaaaaggg gttactttga ataatgcagt tgaatttgct aaagaacaga acaaacccct
      541 aaaaataaaa agataaccat agataaatat ggtcgtgtaa aaatggataa aacttttctt
      601 tatcgaaaag ctaaagatgg aaaatctgca tatatttatt ctgaagggag tgatcaatat
      661 aaagaactaa ctgggaaaga atttgatcct aaaactacaa atattatgaa aggtagaaaa
      721 ttagggacag atggtaagat atcaattgat tttggtgaga ttagtaactc aggaaaaaat
      781 gcagctatta agtcccttaa aaatgaaagt ccttgaatta aatcccaaaa atgcttttaa
      841 aggatggaat gagttaagca aaatagctaa aactgggaaa gtactaggat ggacaggtaa
      901 aattgctaat atttacatga atggaaaaaa agatcttttt gatgataaaa catcttcaat
      961 agatcaaaaa atcaaaaact ttgctatagg ttcaggtgta gatgcattgt ccggtgcagc
     1021 aatagggact ttggtaggag gacctgtggg aaccgttgtt ggagcaggtg cagggctggc
     1081 atttgatttt gctttgaatt ttaaagttaa ggagttgggt aatgaatcaa ttacaggttt
     1141 tgctaaaaaa ggtttaaaaa atcttgcagg aggattagga ttttgaaaaa aagtataaat
     1201 gtaagtaggt atcatggacc tttaattgaa tttgaggttg atgcaagtta tttaagaatt
     1261 ttttcatata tcggtttacc tttatcattg ttttggtatt ttgttactca taacagtgat
     1321 gactttggaa tattaaaatc gatattttta atttgtatta tagtcaattt aatcgggctc
     1381 atattaggat taatacaaaa gtttactaaa aaacttatca aatctctatc attttttgta
     1441 ctttcttagc gtt
//
LOCUS       DATRQA010000233         1443 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.234, whole genome shotgun sequence.
ACCESSION   DATRQA010000233 DATRQA010000000
VERSION     DATRQA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.234"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            125..1408
                     /locus_tag="VBD27_002061"
     CDS             125..1408
                     /locus_tag="VBD27_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO7026026.1"
                     /translation="MSLYYTEKAHAIAGPSDRQYVENPNPHIIVNVTGTDQNGNSILP
                     HYIEVNVKMGQTLSKEEILDYIARNLNSSVGGESKNVQYSNIEFKESAYLKRQLDDGK
                     TEEIAIDNDGVTVPKDGPNKFWIDVPVTCTVTPIVTETHEVRWGTPVAISHRIYFVEE
                     SSGKVLDEYTNLHTADSELNGYRVGDYITDYALSKSAYEAFLNSRLDKEGYKLQHRIS
                     TNVRQNLQINKLIFNYDFNEENIYYQIGNIRPLLSRSSAEVESDIITERYYVSKNAKS
                     LARTESTISIKMVDAKTEQPLFNHTLTGYQLVTVSHVYNRLFEENLIPTTKSGERYFI
                     QNMKKTAEQEYTVYLSETPYSKENAPVISYDARPVDWDYHSGASGSLENQPNIYTEED
                     STEFLGNKPQAACYPNKQFACENTDSKYNYSYLEK"
BASE COUNT          517 a          260 c          236 g          430 t
ORIGIN      
        1 atcattcact gctttatttt aggataacta aacaatttat tgtttaaaat gaaaggattt
       61 atgaatgtct aggaaaacat ttaaacgtat actatcgatt ggagtttgca cgctcgtact
      121 atcgatgagt ctttattaca ctgaaaaagc ccacgctatt gctggtccta gtgaccgcca
      181 atacgtagaa aacccaaatc ctcacattat tgtaaatgtt acaggtactg atcaaaacgg
      241 aaatagcatt ttaccgcatt atatcgaagt caatgtaaag atgggacaaa ctttaagtaa
      301 agaagaaatt ctagattata ttgctcgaaa tttaaactct agtgttggag gagaaagtaa
      361 aaacgttcaa tacagcaaca tcgagtttaa ggaaagtgct tatctgaagc gtcaattaga
      421 tgatggcaag acagaagaaa tagcaattga taacgacggt gttactgtac ctaaagacgg
      481 tccaaacaaa ttttggattg acgttccagt aacttgtact gttactccta tcgtaacaga
      541 aacacatgaa gttcgatggg ggactccagt cgctatatca caccgtattt actttgttga
      601 agaatcttct ggaaaagttt tagatgaata cacaaatcta cacactgctg attcggaact
      661 taacggttat cgtgttggag attatatcac agactatgca ctttctaagt ctgcttacga
      721 agctttttta aactctcgtt tagataaaga aggttacaaa cttcaacatc gtattagcac
      781 gaatgtacga caaaaccttc aaattaataa attgattttc aattatgact ttaatgagga
      841 aaatatttac taccaaatcg ggaatatccg tccactatta agtcgctcat cagctgaagt
      901 agaatctgac atcattacag aacgctacta tgtttctaaa aatgctaaaa gtttagcacg
      961 tacagaatca accatttcga ttaaaatggt tgatgccaaa actgaacaac cgctatttaa
     1021 ccacacatta actggttatc aattggtaac tgtctcccat gtctataaca gactctttga
     1081 agaaaatctt atcccaacta caaaatcagg agaaagatat tttattcaaa atatgaaaaa
     1141 aacagctgaa caagaatata ctgtttatct ttcagaaaca ccttattcta aagagaacgc
     1201 tcccgtaatt tcttatgatg caagacctgt tgattgggat tatcactcag gcgcttcagg
     1261 atcacttgaa aatcagccta acatctatac tgaagaagat tcaactgaat ttttgggtaa
     1321 taaaccacaa gcagcttgtt atccaaacaa acaatttgct tgcgaaaata ctgactctaa
     1381 atacaactat agctatttag aaaaatagtt ataaccgata gggttaatct ctcattatct
     1441 tat
//
LOCUS       DATRQA010000234         1392 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.235, whole genome shotgun sequence.
ACCESSION   DATRQA010000234 DATRQA010000000
VERSION     DATRQA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.235"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            53..706
                     /locus_tag="VBD27_002062"
     CDS             53..706
                     /locus_tag="VBD27_002062"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO7026027.1"
                     /translation="MKHSSWHDLIKRELPNHYYNKINTFMDAVYESGIVYPPRDKVFN
                     AIQITPLENVKVVIIGQDPYHGPQQAQGLSFSVPDNLPAPPSLQNILKELAEDIGSRS
                     HHDLTSWAQQGVLLLNACLTVPEHQANGHAGLIWEPFTDAVIKVVNQKETPVVFILWG
                     GYARKKKSLIDNPIHHIIESPHPSPLSAYRGFFGSRPFSRTNHFLEEEGINEIDWLN"
     gene            complement(772..1392)
                     /gene="plsY"
                     /locus_tag="VBD27_002063"
     CDS             complement(772..1392)
                     /gene="plsY"
                     /locus_tag="VBD27_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO7026028.1"
                     /translation="MIIAYLLGSIQTGLWIGKYFYQVNLRQHGSGNTGTTNTFRILGV
                     KAGIVTLTIDILKGTLATLIPIILGVTTVSPFFIGFFAIIGHTFPIFAQFKGGKAVAT
                     SAGVLLGFAPSFFLYLLVIFLLTLYLFSMISLSSITVAVVGILSVLIFPLVGFILTDY
                     DWIFTTVVILMALTIIIRHQDNIKRIRKRQENLVPFGLNLSKQKNK"
BASE COUNT          491 a          249 c          253 g          399 t
ORIGIN      
        1 ataaaatatt gctatactag taatacagat atctaaaagt aaggagttat aaatgaaaca
       61 ttccagttgg catgatttaa taaaaagaga actacccaat cattactaca ataaaattaa
      121 tacatttatg gatgcagttt acgaaagcgg aatcgtttat cctccaagag ataaggtgtt
      181 caatgccata cagataacac cactagaaaa cgtaaaagta gtgattattg gccaagatcc
      241 ttaccatggc cctcaacaag ctcaaggact ctcattttct gttccagaca atttaccagc
      301 tcctccttca cttcaaaata ttctaaaaga attagcagaa gatattgggt cacgttcaca
      361 tcatgactta acatcatggg cgcaacaagg agtgctgcta ttgaatgctt gtttaacagt
      421 tccagaacac caagctaatg gtcatgctgg gcttatatgg gaacctttta ctgatgctgt
      481 catcaaagtg gttaaccaaa aggaaacccc agtagtcttt attctgtggg gtggctatgc
      541 tcgaaagaaa aagtcgctaa ttgataatcc tattcatcat attattgaga gtcctcatcc
      601 gagtccccta tctgcttatc gtggtttctt tggtagcaga cctttttcta gaacaaatca
      661 tttcttagaa gaagaaggaa taaatgagat tgattggtta aattagaagg gaaatctact
      721 ctcgaaacat tgttagtctt ataataaaaa accgctagta gcggtttttt attatttatt
      781 tttttgttta cttaggttta agccaaacgg aaccaaattt tcttgccttt ttcgaatacg
      841 tttgatatta tcctgatgtc ggataataat cgtgagggcc attaagataa ccacagtggt
      901 aaatatccag tcatagtccg ttaatataaa acctactaat ggaaaaatta agacactaag
      961 gatacctacg acagctactg taatactgga tagcgaaatc atgctaaaaa gataaagcgt
     1021 taataaaaag atcactaata agtaaaggaa aaaagaaggt gcaaatccta gcaaaacccc
     1081 agcgcttgta gcaacggctt tcccaccttt gaattgtgca aaaataggga aagtgtgacc
     1141 tataatagca aaaaaaccga taaaaaatgg agatactgtc gttacaccta atatgatagg
     1201 aataagtgtt gctagagtcc cttttaaaat gtcaatagtc aaagtgacaa tgccagcttt
     1261 tacacctaaa atacggaagg tgttcgtggt gccagtatta ccactaccgt gttgacgaag
     1321 attgacttgg taaaaatatt taccaatcca aagcccagtt tggatagagc ctaacagata
     1381 ggcaatgata at
//
LOCUS       DATRQA010000235         1382 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.236, whole genome shotgun sequence.
ACCESSION   DATRQA010000235 DATRQA010000000
VERSION     DATRQA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.236"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..327
                     /locus_tag="VBD27_002064"
     CDS             <1..327
                     /locus_tag="VBD27_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO7026029.1"
                     /translation="ITLQSLLKEIGIIQSGGAIKKFLADNQVLFNGDLENRRGKKLRL
                     GDIITIPDQNIEIIIRKPSDQEIEERNVEIAEKQRVSAIVKEMNKNTNKDKSKTSKKP
                     VRFPGT"
     gene            330..>1382
                     /gene="recF"
                     /locus_tag="VBD27_002065"
     CDS             330..>1382
                     /gene="recF"
                     /locus_tag="VBD27_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO7026030.1"
                     /translation="MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIY
                     FLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGHLNLDIQLSEKGRITKVNHLKQA
                     KLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKSTYLAELSNYNHVLKQR
                     NTYLKTTNNVDKTFLTVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHL
                     SIHYKSSIEFTDKSSIREHFLNQLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFG
                     SQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQLKLLEGIKENVQTF
                     ITTTSLEHLSALPDQLK"
BASE COUNT          523 a          242 c          217 g          400 t
ORIGIN      
        1 attactttac aatcacttct aaaagagatt ggtatcattc aaagtggcgg agccattaaa
       61 aaatttttag cggataatca agtattgttt aacggtgacc ttgaaaatag gcgtggaaag
      121 aaactccgcc tgggggatat tattactatt cctgatcaaa atatcgagat cattataagg
      181 aagccatcag atcaagaaat tgaagaacgt aatgtagaaa tagctgaaaa acagcgtgtt
      241 tcagcaatcg ttaaagaaat gaataaaaac accaacaaag ataaatcaaa aactagcaaa
      301 aaaccagttc gctttccagg gacataaaaa tgtggataaa aaatatttct ttaaaacatt
      361 atcgtaatta tgaagaagca caagtagatt tttcacccaa cctcaatatt ttcattggcc
      421 gcaatgctca aggaaagaca aattttttag aagcaatcta cttcttggct ttgactcgaa
      481 gtcaccgtac ccgttctgat aaagaacttg tacattttaa acaccatgat gtacaaatta
      541 caggtgaggt aatccgcaaa agtggccatc ttaacttaga tattcaatta tctgaaaaag
      601 gaagaatcac taaagttaac catcttaaac aggcaaaatt atctgattat attggggcta
      661 tgactgtcgt actttttgcc cctgaagatc ttcaattagt taaaggtgca cctagtctaa
      721 gacgaaaatt tttagatata gatattggac aaattaagtc aacgtactta gctgaacttt
      781 caaattacaa tcatgtatta aaacaaagaa acacttatct aaaaactact aataacgtcg
      841 acaaaacttt tttaacagtc cttgatgaac aattagctga ttatggaagt cgtgtcattg
      901 aacaccgttt tgattttatc caagccctaa atgatgaagc ggacaaacat cactacatta
      961 tcagtactga gttagaacat ttatctattc actacaaatc gtcaatagag tttacagata
     1021 agtcaagtat tagggaacat tttttaaacc aattatctaa aagccactca agagacatct
     1081 ttaagaaaaa cacaagcatc ggacctcatc gtgatgatat tactttcttt attaatgata
     1141 tcaatgctac ttttggtagc caagggcaac aaagaagcct tattttatca ttgaaattag
     1201 cagaaattga gctaataaaa accgttacca acgattatcc gattctctta cttgacgacg
     1261 tcatgagtga acttgacaac catcgtcaac taaaactctt agagggaatc aaagagaatg
     1321 ttcaaacatt tattactaca acaagtttag aacatttaag tgctttacct gatcaactca
     1381 aa
//
LOCUS       DATRQA010000236         1379 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.237, whole genome shotgun sequence.
ACCESSION   DATRQA010000236 DATRQA010000000
VERSION     DATRQA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.237"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..258)
                     /locus_tag="VBD27_002066"
     CDS             complement(<1..258)
                     /locus_tag="VBD27_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA processing protein DprA"
                     /protein_id="HEO7026031.1"
                     /translation="MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSK
                     CKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNYPLELKE"
     gene            complement(396..>1379)
                     /locus_tag="VBD27_002067"
     CDS             complement(396..>1379)
                     /locus_tag="VBD27_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7026032.1"
                     /translation="LTTSQAVLAKEKSQTVTIKNNYSVYIKKEKRDKPDNKKQISETL
                     KVPLKPKKVVVFDMGALDTITALGAEKSVIGIPKAKNALSLLPNNVKSVYKAKRYQDV
                     GSLFEPNFEAIARMQPDVVFLGARMASVDNIEKLKEAAPKAALVYAGVDSKKVFDKGV
                     AERVTMLGKIFDQNKKAKTFNKDIAQAVLKLQKTIEKKGKPTALFVMANSGELLTQSP
                     SGRFGWIFSVGGFKAVNENEKLSSHGTPVSYEYIAEKNPNYLFVLDRGATIGQGASSK
                     ELFNNDVIKATDAVKNKRVHEVDGKDWYINSGGSRVTLRMIKDVQNFVDNR"
BASE COUNT          420 a          234 c          222 g          503 t
ORIGIN      
        1 ttcttttaac tctaaaggat agttagaatc caaaatcgat agaactggaa attttttgaa
       61 ttcttgtcga agttttttaa ggtctagctg tttataattt tctataaaga aagtaggatt
      121 tttacatttt gatactacgg ccatattgcg aacagataga gaagttaaac tattcttttt
      181 gagatagttg ataatattgt tgatattaag atttgttagt ccagcttttt taagcttaaa
      241 tagttcaaaa tgattcataa ggttacctcc attgactata ttcgaaaata atagacaaaa
      301 taaagtgata agatcaatct atactctgaa gcattgataa tagctatttt ttcaaaaaaa
      361 taagactagg atttttccta gcctttgaca aaaaattaac gattatcaac aaagttctgt
      421 acatctttaa tcatacggag tgttactcgg cttccgcctg aattgatata ccaatctttt
      481 ccatctacct catgaacacg tttgtttttg acagcatcag ttgctttaat aacatcgtta
      541 ttaaaaagtt cttttgatga agctccttgt ccaatagtcg ctccacgatc taaaacaaag
      601 agatagttag gatttttttc agcgatgtat tcataagata cgggagtacc atgtgaactt
      661 agtttttcat tttcattgac tgctttaaat ccacctacag agaaaatcca accaaaacga
      721 ccagaaggtg attgagttaa aagttcaccg ctgtttgcca ttacaaatag agctgtaggt
      781 ttaccttttt tctcaatagt tttctgcaat ttaagaacag cttgtgcgat atctttatta
      841 aaggtttttg cctttttatt ttggtcgaag attttcccta acattgtgac acgctcagca
      901 actcctttgt caaatacttt ttttgagtcg actccagcat atactaatgc tgctttaggt
      961 gcagcctcct ttaatttttc aatattatca acagaagcca tacgtgctcc taggaaaacc
     1021 acatcaggtt gcatacgagc aatagcttca aagtttggtt cgaagagact tcctacgtct
     1081 tggtatctct tagctttata aacagatttg acgttattgg gcaataaact tagagcattt
     1141 ttagccttcg ggataccaat aacagatttt tcagctccta aagctgtgat agtatccaaa
     1201 gctcccatat caaaaacaac tacttttttg ggttttaaag gaactttaag tgtctcgctg
     1261 atttgctttt tattatccgg cttgtctctt ttttcttttt taatatagac cgaatagttg
     1321 ttttttatgg taacagtttg tgatttttct ttagctaaaa cagcttgaga agtggttaa
//
LOCUS       DATRQA010000237         1371 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.238, whole genome shotgun sequence.
ACCESSION   DATRQA010000237 DATRQA010000000
VERSION     DATRQA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.238"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            98..>1371
                     /locus_tag="VBD27_002068"
     rRNA            98..>1371
                     /locus_tag="VBD27_002068"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          373 a          283 c          414 g          301 t
ORIGIN      
        1 gacgaacgaa tgtgcagggt ggatttacat tgtaaatccg tcaatgacaa aaaacaataa
       61 atctgtcagc gagacagaaa agagcaaagc tcaaactttt aatgagagtt tgatcctggc
      121 tcaggacgaa cgctggcggc gtgcctaata catgcaagta gaacgctgag gtttggtgtt
      181 tacactagac tgatgagttg cgaacgggtg agtaacgcgt aggtaacctg cctcatagcg
      241 ggggataact attggaaacg atagctaata ccgcataaga gtaattaaca catgttagtt
      301 atttaaaagg agcaattgct tcactgtgag atggacctgc gttgtattag ctagttggtg
      361 aggtaaaggc tcaccaaggc gacgatacat agccgacctg agagggtgat cggccacact
      421 gggactgaga cacggcccag actcctacgg gaggcagcag tagggaatct tcggcaatgg
      481 acggaagtct gaccgagcaa cgccgcgtga gtgaagaagg ttttcggatc gtaaagctct
      541 gttgttagag aagaacgttg gtaggagtgg aaaatctacc aagtgacggt aactaaccag
      601 aaagggacgg ctaactacgt gccagcagcc gcggtaatac gtaggtcccg agcgttgtcc
      661 ggatttattg ggcgtaaagc gagcgcaggc ggttctttaa gtctgaagtt aaaggcagtg
      721 gcttaaccat tgtacgcttt ggaaactgga ggacttgagt gcagaagggg agagtggaat
      781 tccatgtgta gcggtgaaat gcgtagatat atggaggaac accggtggcg aaagcggctc
      841 tctggtctgt aactgacgct gaggctcgaa agcgtgggga gcaaacagga ttagataccc
      901 tggtagtcca cgccgtaaac gatgagtgct aggtgttagg ccctttccgg ggcttagtgc
      961 cgcagctaac gcattaagca ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag
     1021 gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgaag caacgcgaag
     1081 aaccttacca ggtcttgaca tccttctgac cggcctagag ataggctttc tcttcggagc
     1141 agaagtgaca ggtggtgcat ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc
     1201 ccgcaacgag cgcaacccct attgttagtt gccatcatta agttgggcac tctagcgaga
     1261 ctgccggtaa taaaccggag gaaggtgggg atgacgtcaa atcatcatgc cccttatgac
     1321 ctgggctaca cacgtgctac aatggttggt acaacgagtc gcaagccggt g
//
LOCUS       DATRQA010000238         1366 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.239, whole genome shotgun sequence.
ACCESSION   DATRQA010000238 DATRQA010000000
VERSION     DATRQA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.239"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..723
                     /gene="recX"
                     /locus_tag="VBD27_002069"
     CDS             <1..723
                     /gene="recX"
                     /locus_tag="VBD27_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEO7026033.1"
                     /translation="NTENLYITEDTIVHFMLSKGMIINAEKLENIKKFAQLSYGKNLG
                     LYYISFKQRTEKEVIKYLQEHDIDSKIIPQIIDNLKSENWINDKNYVQSFIQQNLNNG
                     DKGPYVIKQKLLQKGIKSKIIESELKAINFQDLASKISQKLYKKYQNKLPLKALKDKL
                     MQSLTTKGFDYQIGHTVIQNLEIEKDQELEDDLIYKELDKQYQKLSKKHDQYELKQRI
                     INVLMRKGYQYEDIKSALREYL"
     gene            807..1340
                     /locus_tag="VBD27_002070"
     CDS             807..1340
                     /locus_tag="VBD27_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEO7026034.1"
                     /translation="MRLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRETGVSYYCNLASPYILDPEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEQHKAQMNYPTDIDYILKENVKILVEWINENKGPFS
                     SSYINIWYKRYLELKKR"
BASE COUNT          567 a          201 c          217 g          381 t
ORIGIN      
        1 aataccgaaa atttatatat aacagaagat actatcgttc attttatgtt aagtaaaggg
       61 atgataatta atgccgagaa acttgaaaat attaaaaaat ttgcccaact atcatacggt
      121 aaaaatctag gtctttatta tatttctttc aagcagagaa ccgagaaaga agttattaaa
      181 tatcttcaag aacatgatat tgattctaaa attattccac aaattattga taatctaaaa
      241 agtgagaatt ggataaacga taaaaactac gtgcaatctt tcatacagca aaatctaaat
      301 aatggcgata aggggccata cgtgataaaa caaaaattat tgcaaaaagg tatcaaaagt
      361 aaaataattg aatctgaact taaagctata aactttcaag acttagcatc caaaataagc
      421 caaaaactct ataaaaaata tcaaaacaaa cttccactaa aagcattaaa agataaattg
      481 atgcagagct taactacaaa aggatttgat tatcagatag ggcataccgt aattcaaaac
      541 cttgaaattg aaaaagatca agaacttgaa gatgacctca tttataaaga attagataaa
      601 caataccaaa aattatcaaa aaaacatgac cagtatgaat taaagcaacg tataatcaat
      661 gtcttaatgc gtaaaggata ccagtacgaa gatatcaaaa gtgctttaag agaatattta
      721 taagataata actctcaaac gatgtatttt acagaaaatt atgataaact ataacagacg
      781 tataaattgt agaaagttgg taggctatga gattacctaa agaaggcgac tttattacaa
      841 ttcaaagtta caaacatgat ggtagtttac accgaacttg gcgtgacacc atggtattaa
      901 aaacaaccga aaatgccctc attggtgtta atgatcatac tttagtaaca gaaaatgatg
      961 gtcgacgctg ggtgacacga gagcctgcaa tagtatactt tcataaaaaa tattggttta
     1021 acattatcgc tatgatacgt gaaactggtg tctcctacta ttgtaatcta gcaagtccgt
     1081 atatcttgga tcctgaagca ctcaagtaca tcgactacga ccttgatgtc aaagtatttg
     1141 cagatggtga aaaaagacta ctggatgtgg acgaatatga acagcataaa gctcagatga
     1201 actatcctac cgatattgat tatatattaa aggaaaatgt aaaaatattg gtagaatgga
     1261 taaatgagaa taaaggcccc ttttcatcat catatatcaa tatctggtat aaacggtacc
     1321 ttgaattgaa aaagcgctaa ctaacacact aaatagtgtg ttttta
//
LOCUS       DATRQA010000239         1333 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.240, whole genome shotgun sequence.
ACCESSION   DATRQA010000239 DATRQA010000000
VERSION     DATRQA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.240"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(200..523)
                     /locus_tag="VBD27_002071"
     CDS             complement(200..523)
                     /locus_tag="VBD27_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO7026035.1"
                     /translation="MINSTVFLAILLGFVVSWVPRVAPFIFVKYKGLPNSLVKFLKFL
                     PLSILFALTFSSFFSVKLGQLPTLKWLDLLAGIPTLVVTIRYRNLFYTVLTGIVTMAL
                     LRLLF"
     gene            complement(513..1205)
                     /locus_tag="VBD27_002072"
     CDS             complement(513..1205)
                     /locus_tag="VBD27_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO7026036.1"
                     /translation="MEKGFKEGVKDALPTALGYISIGLAFGIVASASDLSAIEVGLMS
                     ALVYGGSAQFAMCALLLAKADLMTITMTVFLVNLRNMLMSLHATTIFKSAHLMNQLAI
                     GTLITDESYGVLLGEALHHKVVSPSWMHGNNVMSYLTWVISTIIGTLLGSTIPNPEMF
                     GLDFALVAMFIGLFVFQLFGMLSDGKRLVVYVLASVGLSYFLLSTFLSGALSVLLATV
                     VGCSVGVVLDDK"
BASE COUNT          506 a          263 c          193 g          371 t
ORIGIN      
        1 agcatgtaga tatctttaaa aagcaatctt acaatcaact catattatca agatacagaa
       61 aaatctgttg atattattaa caattagtca aactattaca catcggctat caaaagttcc
      121 tgtctagaca aaaacacaaa aaacgtatct agaaatagat gcgttttttg tgtttttatt
      181 aatcttccat tcctacacgt taaaatagta atctcaataa agccatagta acgataccag
      241 taagaacagt gtaaaataaa tttcgatatc gaatagtgac aactagagtt ggtatgccag
      301 ccaataaatc taaccatttt agtgttggca attgtcctaa tttaactgaa aaaaagctag
      361 agaatgttag ggcaaataaa atagaaagag gaagaaattt taaaaatttc acaagactat
      421 ttggtagccc tttgtatttt acaaatatga atggagcgac tcttggcacc caagaaacaa
      481 caaaacctaa aagaatagca agaaaaacgg tactatttat catctaaaac aactcctaca
      541 ctacatccaa caacagtcgc aagcaatact gataaggctc ctgacaaaaa agtggataat
      601 aagaagtaac ttaaacctac agaagcaaga acatatacaa caagtcgctt tccatcagat
      661 aacatcccaa acaactgaaa tacaaagaga cctataaaca tggcgactaa tgcaaagtct
      721 aaaccaaaca tttctggatt aggaatagta ctccccagta aagttcctat tatcgttgag
      781 attacccatg tcaaataact catcacatta ttaccatgca tccaactagg cgatacgact
      841 ttgtgatgca atgcttctcc aagtaaaacg ccgtagctct catctgttat tagagttcct
      901 atggctaatt gattcattag gtgagcagac ttgaagatag tagtcgcgtg taaactcatt
      961 aacatatttc tcaaattaac taaaaatact gtcatcgtaa tagtcatcaa gtctgctttt
     1021 gctaataata gcgcacacat agcaaactga gcagatcccc catataccaa ggcactcata
     1081 agaccaactt ctattgcaga taaatctgat gctgaagcaa ctatcccaaa cgccaagcct
     1141 attgagatat accctaaagc tgtgggcaag gcatctttca ctccctcttt aaatcctttt
     1201 tccatcatat ccccctaaat tttcagtata ttcagttatt atagcacaac ggtgattact
     1261 aagaaagaag aggaatgatt atttagcttt gccagcaatt agactaataa atatcctgta
     1321 attattctat aaa
//
LOCUS       DATRQA010000240         1333 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.241, whole genome shotgun sequence.
ACCESSION   DATRQA010000240 DATRQA010000000
VERSION     DATRQA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.241"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(46..1251)
                     /locus_tag="VBD27_002073"
     CDS             complement(46..1251)
                     /locus_tag="VBD27_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEO7026037.1"
                     /translation="MSQKSNYFKGQIDYAVVIPVFFLLMIGLASIYVATMNDYPSNIY
                     IAMFQQVSWIIMGCIIAFVVMLFSTEFLWKATPYLYALGLTLMVLPLIFYSPQLFAAT
                     GAKNWVTIGSVTLFQPSEFMKISYILMLSRITVSFHQKNRKTFQDDWKLLGLFGLVTL
                     PVMILLMLQKDLGTALVFLAILSGLILLSGISWWIILPILSTIVLFIASFLMIFISPN
                     GKEWFYNLGMDTYQINRLSAWIDPFSFADSIAYQQTQGMVSIGSGGVTGKGFNILELS
                     VPVRESDMIFTVIAENFGFIGSAIVLGLYLIIIYRMLRITIESNNQFYTFISTGFIMM
                     IVFHVFENIGAAVGILPLTGIPLPFISQGGSSLLSNLIGIGLVLSMSYQNTVRQAKAN
                     GSHLKYRSI"
BASE COUNT          507 a          247 c          200 g          379 t
ORIGIN      
        1 atttcgatta ttaaaaggct ggattatctt tccaaccttt ttttgttata tactacgata
       61 cttcaaatgt gatccattag ccttggcctg acgaactgta ttttgataag acatggataa
      121 gaccaatcca attccaataa gattacttag taatgaactt ccaccttgcg aaataaacgg
      181 aagtgggatt cctgttagag gtaatatacc aactgctgca ccaatatttt caaaaacatg
      241 gaaaacaatc atcatgataa aaccagttga tataaaagta taaaattgat tattagattc
      301 aatggtgatc cttaacatac ggtaaatgat aatgagatat aaccctaaaa caatcgccga
      361 accaatgaag ccaaaattct cagcaatgac agtaaaaatc atgtcacttt cacgaacagg
      421 aaccgagagc tctaaaatat taaaaccttt acctgttacc ccgccacttc caattgatac
      481 cataccctgt gtttgttgat aagcaattga gtctgcaaaa ctaaatggat caatccaagc
      541 agataaacgg ttgatttgat atgtatccat ccccaaatta taaaaccatt ccttgccgtt
      601 tggtgagata aaaatcatca aaaagctagc tataaaaaga acgatagtag ataaaattgg
      661 caagattatc caccaagata tgcccgataa aaggattaaa cctgacaaaa tggcaaggaa
      721 aactaatgct gttcctaaat ctttttgtaa cattagaaga atcatcactg gcaatgtaac
      781 caaaccaaac aatcctagca acttccaatc atcttgaaaa gttttgcgat ttttctgatg
      841 aaaactaaca gttattcgag ataacattaa aatataggaa atcttcataa actctgacgg
      901 ttgaaacaaa gttaccgagc caatggtcac ccaattttta gcacctgttg ccgcaaaaag
      961 ctgtggacta taaaaaatta agggaagaac cattagggtt agtcctaaag catacagata
     1021 gggcgtggct ttccataaaa attcagtact gaaaagcata acgacaaatg caataataca
     1081 ccccataata atccaagaca cctgctgaaa catcgcgata tagatattag aaggataatc
     1141 attcatcgtt gcaacataaa ttgaggctaa accaatcatt aataaaaaaa atactggtat
     1201 cacaacagca taatcaattt gccctttaaa atagtttgat ttttgtgaca taggacttcc
     1261 ttttttattc atggtaatta ttataacatt ttttcagaat ctgacttata ttttttgcaa
     1321 taaaaaatgc aac
//
LOCUS       DATRQA010000241         1330 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.242, whole genome shotgun sequence.
ACCESSION   DATRQA010000241 DATRQA010000000
VERSION     DATRQA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.242"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            307..1329
                     /locus_tag="VBD27_002074"
     CDS             307..1329
                     /locus_tag="VBD27_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7026038.1"
                     /translation="MNIIIGTSLLILVLAIFTLFNYKAPYGTKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVTIAIGVTPGYAVLIGLSVSGTG
                     IIPGFLAGYLVGFLVKWMERNIPGGLDLISIIIIGAPLTRLVAKLLTPLINSTLLTIG
                     DILTSGAHSNPILMGVILGGTIVVVATAPLSSMALTAMLGLTGMPMAIGALSVFGSSF
                     MNGVLFHKLKLGSRKDNIAFAVEPLTQADVTSANPIPIYVTNFVGGAACGILIALMKL
                     VNDTPGTATPIAGFAVMFAYNPMIKVLITALGCIILSLLAGYFGGIVFKDYKLVTKEE
                     LQARDN"
BASE COUNT          389 a          210 c          263 g          468 t
ORIGIN      
        1 gacaatatca gactatttta aaagattaat gtaataaaaa attatcgtaa taaaaaaaag
       61 ttaagaaaat aaaaaattat tattgactat ggttatgctt ttaggtaaaa tgtgattata
      121 ttagcaacta aggagtaatc catgagataa gcctaataat tatgtcggac ttctttgttg
      181 caccttatcc agttcgtagt acaattaagt ttcagtatta cctaaaggcg cctttattga
      241 aattgaagca gtagtacaca gacactgaga ataaaaaact taactatttt tagcgggagt
      301 atatttatga atatcattat tgggaccagt cttttaatac ttgtattagc gattttcact
      361 ttatttaatt ataaagcacc ctacggaaca aaagcaatgg gagcattggc atctgcggct
      421 tgtgcatcct tccttgtaga agcttttcaa gattctttct ttggtaaagt tttgggattt
      481 caatttttaa gtgaagtggg tggtgctaat ggttcacttt ctggtgtagc tgcagctata
      541 ttggttacta ttgctattgg tgtaacccca ggatacgcag ttttaattgg tttatctgtt
      601 tctggaacag gtattattcc tggtttcctt gcaggatatt tagtgggatt tcttgttaag
      661 tggatggaaa gaaatattcc tggtggttta gatcttattt caattattat tattggtgca
      721 ccattaacac gtcttgtggc caaactgttg acgccgctta ttaatagcac tttgctcacc
      781 attggagata tcttaacatc aggtgctcat agcaacccta ttttaatggg ggttatactt
      841 ggcgggacaa ttgtagtagt ggcgacagca ccactttctt ctatggcatt gacagctatg
      901 ctaggattaa ccggaatgcc tatggctata ggagccttgt ctgtctttgg ttcgtcattt
      961 atgaatggtg tacttttcca taaattaaaa cttggaagtc gtaaagataa tatagctttt
     1021 gctgttgagc ctctaactca agctgacgtg acttcagcta accctattcc aatctatgtc
     1081 actaattttg ttggtggtgc agcttgtggt attttaattg ccttgatgaa attagttaat
     1141 gatactcctg gaacagcgac accaattgca ggatttgctg tcatgtttgc ctataaccca
     1201 atgataaaag tactaataac cgctctaggt tgtattatcc tatctttact agcaggctat
     1261 tttggaggca ttgtttttaa agattataaa ttagttacaa aagaagagct acaggctaga
     1321 gataactagt
//
LOCUS       DATRQA010000242         1330 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.243, whole genome shotgun sequence.
ACCESSION   DATRQA010000242 DATRQA010000000
VERSION     DATRQA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.243"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(7..>1330)
                     /gene="gtfB"
                     /locus_tag="VBD27_002075"
     CDS             complement(7..>1330)
                     /gene="gtfB"
                     /locus_tag="VBD27_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEO7026039.1"
                     /translation="ILFDFFDKKSKDLYYSLITSGLHGNAVVINDDGFLPQNINSPYS
                     FFCNMEGKNGNPLYFNQVPLPDLWEIKGNNIEAEIWDFSIKRAKIFYQEPKYKRQVKN
                     IDWFDNNKKVRYTDHYNRFGWCFARTHFDKNQNVTTKSYFDKDGKEVIVENFRTGVII
                     LNWLNKDYFFDNRVAFLNFYFSLMGWNLSRIWYNSLSTPFFVSYRMTYPGEDILFWQE
                     DIEDTIPANMRVLLESTNTRTQKVIVQKKNTYHKIKSMLPKEQQEKIGYLGFIYPNKK
                     NNKGRKDIFILTNSDQIEHLEVLVHHLSDYHFHIAAYTEMSFKLMSFSQEQNVTLYPN
                     ISRTDLDNLFEICDIYFDINHGNEVDDVIRRAFEYNHLIFAFDNTCHNRELVLDSNII
                     SHTTCEQLINLMKNLSGSIMYLLEQQREQTSNETKERYKEILGGYGNA"
BASE COUNT          448 a          222 c          170 g          490 t
ORIGIN      
        1 tttcttttag gcatttccat accctcctaa tatttcttta taacgctctt ttgtttcatt
       61 acttgtttgt tctctttgtt gctctagcaa atacataatg gagcctgata aatttttcat
      121 taaatttatc aattgttcac aggttgtgtg agaaatgata ttgctatcta atactaactc
      181 tctgttatga caggtattat caaaagcaaa gataaggtga ttatattcaa atgcccttct
      241 aataacatca tcaacttcat ttccatgatt aatgtcaaaa taaatatcac agatttcaaa
      301 taagttgtct aaatcagttc ttgaaatatt cggatatagc gttacatttt gttcttggct
      361 aaatgacatc aacttaaaag acatttctgt gtacgctgca atatgaaaat gataatcaga
      421 caagtgatga accaacactt ctaaatgttc aatttggtca gagtttgtca aaataaaaat
      481 atctttccta cccttattgt tctttttatt aggatatata aagcctaaat aaccaatttt
      541 ttcttgttgt tctttaggta acattgactt tattttatga tatgtatttt tcttttgaac
      601 aattactttc tgagttctag tattcgtact ttctaataac acgcgcatat tagcaggaat
      661 agtatcctct atatcttctt gccaaaataa aatatcttct cctggataag tcatccggta
      721 agaaacgaaa aatggagttg ataaggagtt gtaccatatt cgagacaagt tccaacccat
      781 aaggctaaaa taaaaattta agaatgcaac tcgattatca aaaaaataat ctttatttag
      841 ccaattaagg attataactc ctgttctaaa attttcaaca ataacttctt taccatcctt
      901 atcgaaataa gattttgtag tcacattttg attcttatca aaatgtgtac gtgcaaaaca
      961 ccatccgaag cgattataat gatctgtgta tctcactttt ttattattgt caaaccaatc
     1021 aatatttttt acttgacgtt tatacttcgg ctcttgataa aaaattttag ctcttttaat
     1081 actaaaatcc caaatttcag cctctatatt atttcccttt atctcccaaa ggtcagggag
     1141 tgggacttgg ttaaaataaa gaggattccc atttttccct tccatattac aaaaaaatga
     1201 ataaggagaa tttatatttt gtggaagaaa accatcgtca ttaatgacca cagcatttcc
     1261 atgtaaacct gaagtaatta aagaataata tagatctttg cttttcttat caaagaaatc
     1321 aaatagtata
//
LOCUS       DATRQA010000243         1305 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.244, whole genome shotgun sequence.
ACCESSION   DATRQA010000243 DATRQA010000000
VERSION     DATRQA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.244"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..321
                     /locus_tag="VBD27_002076"
     CDS             <1..321
                     /locus_tag="VBD27_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEO7026040.1"
                     /translation="SISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDITTLEKGNL
                     PKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGLTSLGIVPD
                     LNKL"
     gene            332..1021
                     /locus_tag="VBD27_002077"
     CDS             332..1021
                     /locus_tag="VBD27_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEO7026041.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAIASVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVNESVATYGDY
                     GNYGKRDRKRK"
     gene            1034..>1305
                     /locus_tag="VBD27_002078"
     CDS             1034..>1305
                     /locus_tag="VBD27_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026042.1"
                     /translation="MKEKENIQKIIIAMIQTVVVYFSASLTLTLITPNFKSNKDLLFV
                     LLIHYIVFYLSDFYRDFWSRGYLEEFKMVLKYSFYYIFISSSLFFI"
BASE COUNT          463 a          175 c          237 g          430 t
ORIGIN      
        1 tcaatatcgg tcgaagatgc taatccaaaa atgtctcaaa aattagcaaa ttcagttaga
       61 gaagcagcag tttcaaaaat caaggcagtt actcaagtag aggatatcac tactcttgag
      121 aagggaaatt tacctaaagc accatcttct cctaatatta aaaagaatgt actaatcggg
      181 tttattgttg gtgcaggatt atcaactatt gttttagtta ttatggggat tttggatgat
      241 cgtgttaata ctgaagaaga tattgaaaag gtcttaggac ttacttcttt aggtatagta
      301 ccagatttga ataaacttta aggagaatat aatgactcgt ttagaaatag ttgatagcaa
      361 gttaagacaa gcaaaaaaaa cagaagaata cttcaatgcg atccgtacaa acatacagtt
      421 tagtggaaag gaaaataaaa ttcttgcaat tgcctctgtt agggaagggg aaggaaaatc
      481 cactacttca acaagtttag ctttatcttt agctcaagca ggatttaaaa cattattaat
      541 tgatgcggat actaggaact ctgttatgtc tggaaccttt aaagcaactg gaactattaa
      601 aggcttgacg aattatttat caggtaatgc agatcttgga gatattatct gtgaaaccaa
      661 tgttcctaga ctgatggtcg ttccttcagg aaaagtacca ccaaatccaa cagcattact
      721 tcagaacgct tattttaata agatgattga agctattaaa aatatatttg attatattat
      781 catcgatact ccacctattg gtttagttgt tgatgccgca ataatcgcta atgcttgcga
      841 tggttttatt ttagtaaccc aagcaggcag aataaaacgt aattatgttg aaaaagcaaa
      901 agaacagatg gaacaaagtg gttcaaagtt cttaggtatt attcttaata aagttaatga
      961 atctgttgct acttacggcg attatggaaa ttacggaaaa agggatagaa aaaggaagta
     1021 agggactctg gtattgaaag aaaaagaaaa tatacaaaag attattatag cgatgattca
     1081 aacagttgta gtttattttt ctgcaagttt gacattaaca ttaattactc ccaattttaa
     1141 aagcaataaa gatttattgt ttgttctatt gatacattat attgtttttt atctttctga
     1201 tttttacaga gacttttgga gtcgtggcta tcttgaagag tttaaaatgg tattgaaata
     1261 cagcttttac tatattttca tatcaagttc attatttttt atttt
//
LOCUS       DATRQA010000244         1277 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.245, whole genome shotgun sequence.
ACCESSION   DATRQA010000244 DATRQA010000000
VERSION     DATRQA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.245"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..>1277
                     /locus_tag="VBD27_002079"
     CDS             <1..>1277
                     /locus_tag="VBD27_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="surface protein"
                     /protein_id="HEO7026043.1"
                     /translation="SIKRKYQVSGKKVLMDNNYRFGSGIKNTRQDAIAIVRLLLEKNK
                     DYLIYMNGLEEPSVLYNPENKRYKFTNNKGKAIGNIPVGLTAFENSAETQEYVLKNIQ
                     KNETIYLGNTRVDNPRVTVNNKKRDTIGVEYGKRITYRIPIYSKQLTVRVSPNFVVDS
                     TNYNYRLSQAPIIAEEGKEGRLYPIDSSISVDGDKIVLNSSGDETEQKELLQNSLYKL
                     HSIDKLDFDLTNADIKELEITGHVASKRDYNLSVAQEKKGVIETKRSLISVDNQKQDQ
                     GIFVEDSKGYIKTPSLSSYGINFAMYDDNQNRLLQGSEYILGRLNADKKVSLYQPNGF
                     WINTQKTIKSLNKEYLNQNAYHIRGNNIIGIDGYSNDIDLNPRLWAYNYKKQVKSNQS
                     LFKLRGLSNKYKYFLIQTKSPKGYLQSNKPFYF"
BASE COUNT          498 a          160 c          231 g          388 t
ORIGIN      
        1 atagtatcaa gcggaagtac caagttagtg gtaaaaaagt ccttatggat aataactata
       61 gatttggttc tggtataaaa aatactagac aggatgctat tgctattgta aggttactgc
      121 ttgaaaagaa taaagattat ttaatttaca tgaatggctt agaagagcca agtgttttat
      181 ataatccaga aaataaacgt tataagttca caaataacaa aggaaaagca attggtaata
      241 ttccagttgg tttaactgct tttgaaaaca gcgcagaaac tcaagaatat gtgttgaaaa
      301 atattcagaa aaacgaaacg atttatcttg gtaatacccg agtagataat cctcgagtaa
      361 ctgttaataa caaaaaaaga gatactatag gagttgaata cggtaaacgt attacttata
      421 ggattccaat ttattcaaag cagctgactg tcagagttag ccctaatttt gtagtagata
      481 gtactaatta taattaccga ctttctcaag cgcctattat tgcggaagaa ggtaaagagg
      541 gacgtttgta tccaatagat agcagtatta gtgtcgatgg tgataaaatt gttttaaata
      601 gtagtggtga tgaaacagag caaaaagaat tattacaaaa tagcctgtat aaacttcact
      661 ctattgataa gttagacttt gatttgacaa atgctgatat taaagagtta gagattacag
      721 gacatgttgc ttcaaaaaga gattataatt tgtctgttgc tcaggaaaag aaaggcgtca
      781 tagagactaa acggagtcta atttctgttg ataatcaaaa acaagatcaa gggatttttg
      841 tcgaggattc taaaggctat ataaagacac ctagtttgag cagttatggt ataaattttg
      901 ccatgtatga cgacaatcaa aatagattat tgcaaggttc agagtatatt ttaggaagat
      961 tgaatgcaga taaaaaagta tctctatatc aacctaatgg cttttggata aatactcaaa
     1021 aaacgattaa gagtttaaat aaagaatatc ttaaccaaaa tgcttaccat attagaggaa
     1081 ataacattat aggtattgat ggctattcaa atgacataga cttgaatcct aggttatggg
     1141 cttataacta taaaaaacaa gtgaaaagta accaatcatt atttaaatta cgaggtttgt
     1201 ctaataagta taagtatttc ttaatacaga caaaatctcc aaaaggctac ttacaaagca
     1261 ataaaccatt ttatttt
//
LOCUS       DATRQA010000245         1262 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.246, whole genome shotgun sequence.
ACCESSION   DATRQA010000245 DATRQA010000000
VERSION     DATRQA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.246"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            212..>1262
                     /locus_tag="VBD27_002080"
     CDS             212..>1262
                     /locus_tag="VBD27_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7026044.1"
                     /translation="MLGENIYLQRTQIGMTQENLSDYLHLTKTTISKWENNQAKPDID
                     YLILMANLFDISLDDLVGYQKTLSDDQRNQLIKDLKIKANVLSERDFFQEVKELSKQF
                     PNDFKTLLIMINMVLSNLTNLNDSEMKEWSLSTLNKIISKTVVESDLQTAIMFKTVVL
                     FHNLEYDEVINLYQNSPYKLGEELLLANSFFAKGDVYRAKQVLQVEIYQQILLVCEYL
                     LSLSTFEVEENKGDIFERLEHMIEAFQLNSLHPNTVIKCYYSLACHYSKSDDDKALGY
                     LKQCFKSLKQLLQRFELHGDTFFYTIDDWLETIPTGIAPPTSQLQVIERVEDLFQNSQ
                     FKELSGRKEFQQIIQK"
BASE COUNT          445 a          178 c          214 g          425 t
ORIGIN      
        1 ttaagggctc ttttataatt cattattttt caatgacaaa tattagtgaa gttaattttt
       61 atttaaccat tgttattatt aatgctgttt tgccacttat taatcaacga aaaagtttct
      121 tctatggaaa cttttttata ttatatttcc tgataggaac ttttattttt gatttagaaa
      181 ctctataatg gacgtgaaag gagattgacc gatgcttgga gagaatatct atttacaacg
      241 tactcagatt ggtatgacgc aagagaattt gagtgattat ttgcatttaa caaaaactac
      301 aatttcaaag tgggaaaata atcaagctaa accggatatt gactatctta ttttgatggc
      361 taatcttttt gatattagct tggatgactt agtcggttat caaaaaacat tgtcagatga
      421 tcaaagaaat cagctcatta aggatctaaa aataaaagca aatgtattgt ctgagagaga
      481 tttctttcag gaagttaagg aattatcaaa acagttccct aacgatttta agactctgtt
      541 gataatgatt aatatggtct tgagcaacct aaccaacctt aatgattcag aaatgaaaga
      601 gtggtcacta agtacactaa ataaaattat tagtaaaaca gtagttgagt ctgacctcca
      661 gacggcgata atgttcaaaa cggtcgttct ttttcacaac cttgaatatg atgaagtgat
      721 aaacctttac cagaatagtc cctataaatt aggtgaagaa ttacttttag ctaatagctt
      781 ttttgcaaaa ggcgatgttt accgagctaa gcaagtatta caggttgaaa tttatcagca
      841 gattctactt gtatgcgagt atctattgag tttatcaact tttgaagttg aagaaaacaa
      901 aggcgatatt tttgaaaggc ttgaacatat gatagaagcg tttcaattaa actctttgca
      961 tcccaacaca gtcatcaagt gttattatag tttagcttgt cattatagta aaagtgatga
     1021 tgataaggca ttaggatatt taaaacaatg ttttaaatct ttaaaacagt tgctccaaag
     1081 atttgaatta cacggtgata ctttctttta taccatcgat gattggttag agacaatacc
     1141 tactggtatt gcaccaccga cgagtcaatt acaggtaata gaacgagttg aggacttatt
     1201 tcaaaattca caatttaaag aactatcagg gcgtaaagaa tttcaacaaa ttattcaaaa
     1261 ac
//
LOCUS       DATRQA010000246         1247 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.247, whole genome shotgun sequence.
ACCESSION   DATRQA010000246 DATRQA010000000
VERSION     DATRQA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.247"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(291..362)
                     /locus_tag="VBD27_002081"
     tRNA            complement(291..362)
                     /locus_tag="VBD27_002081"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(327..329),aa:Arg,seq:cct)
     gene            complement(473..820)
                     /gene="rplS"
                     /locus_tag="VBD27_002082"
     CDS             complement(473..820)
                     /gene="rplS"
                     /locus_tag="VBD27_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO7026045.1"
                     /translation="MNPLIQSLTEGQLRSDIPEFRAGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
BASE COUNT          398 a          247 c          193 g          409 t
ORIGIN      
        1 aaaaaataaa aacgacagat tattcgacaa actttcaaat aatgcagtca ataaaactat
       61 ccgaaaaata actggtcgcg aggtacgagt gcactctctc aggcatacat ttgcaagcta
      121 tctaatttcc atctctcaag ttttagatca cgagaattta aatatcactt tagaagttta
      181 cgcacatcaa ttacaagaac aaaaagatag gaacgataaa cttaatcaga gaaatttggg
      241 gcggatttgg ggcaaaatag ctctaaaccg ctatttacat gcaatgaata tgtcccctgc
      301 cggaatcgaa ccagcaatta ctccttagga gggagttgtt atatccattg aactaagggg
      361 acctagtaaa aaaactgccc acaggcagat tttttacgtc ttggttgtcc agttttaaaa
      421 catagttact atcctcaaac aaccaagcat ttttaaaatc tgatcatcaa aattaacgac
      481 ggatttcttt aatacgtgca gctttacctt gcaatgcgcg taagtagtaa agtttagcac
      541 gacgtacttt accataacga acaacttcga ttttatcaac acgaggagtg tgaattggga
      601 atgtacgctc tacaccgata ccaccagaaa ttttacgtac tgtgtacatt tctgagattc
      661 cttgaccttt acgtgagata acaacacctt caaagatctg aatacgttcg cgagtacctt
      721 caacaacttt agcgtgaaca cgtacagtat caccagcacg gaactcaggg atatcagaac
      781 gaagttgacc ttctgtcaaa ctttgaatta atggattcat ttttattctc cttctcttac
      841 taatcttaag tacttgtctc agcggattag ccgttttttg tgcgtccatt acacactctc
      901 acaattctac caaattttaa cctgcttgta aagctatttt atctagaaga acaaggtgtc
      961 atcctttaga atagttattt gtatattatt gaaatttgat ttactgtttc taggttagct
     1021 tttttctgat ttttgagagt ttaattcatt taatattata tgatttttta aactacgttt
     1081 tctatattag ctttcgtgtc acctgactct ttcccataaa gttgaaatcc acatcaactt
     1141 tttatcttct aatgcatttc accatcgcga cttaaatgct tgtagacatt atcctaacct
     1201 aagttcttac acattcaatt ggattttaca gtattttaat gacaatc
//
LOCUS       DATRQA010000247         1227 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.248, whole genome shotgun sequence.
ACCESSION   DATRQA010000247 DATRQA010000000
VERSION     DATRQA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.248"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..1066)
                     /locus_tag="VBD27_002083"
     CDS             complement(<1..1066)
                     /locus_tag="VBD27_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEO7026046.1"
                     /translation="MKNLNRYVVAVSGVVLHLMLGSTYAWSVFRNPIISETGWDISSV
                     SFAFSLAIFCLGMSAAFMGHLVERFGPRIMGMISAILYGAGNVLTGLAIETQQLWLLY
                     VAYGILGGIGLGSGYITPVSTIIKWFPDRRGLATGFAIMGFGFASLVTSPLAQSLLIR
                     IGVGKTFYILGLVYFFVMMIASQFIKQPPQEKITILTHDGKKNAMNSQIITGLKANVA
                     IKSKTFYIIWLTLFINISCGLGLISAASPMAQDLAGYSAESAALLVGVLGIFNGFGRL
                     LWASLSDYIGRPLTFIILFIVNFIMTSSLFLSFNAIVFAIAMSILMTCYGAGFSLLPA
                     YLSDIFGTKELATLHGYSLTA"
BASE COUNT          469 a          243 c          176 g          339 t
ORIGIN      
        1 atgctgttaa actataacca tgtaaagtag ctaattcctt tgttccaaaa atatcactta
       61 gataagcagg taataaggaa aaacctgcac cataacaagt cattaaaata gacatcgcta
      121 ttgcaaatac aatagcattg aatgacaaaa ataaactaga agtcataata aagttcacaa
      181 taaataatat tataaaggtc aacgggcgtc caatgtagtc agagagactt gcccataaca
      241 gacgtccaaa accgttaaat atccctagta cccctactaa taaggctgca gattctgcgg
      301 aatagcctgc taaatcttgt gccattggtg aagctgctga tattaaacct aagccacacg
      361 aaatattaat aaacaaggtc aaccaaatga tgtaaaaggt ttttgatttt atagcgacgt
      421 ttgcttttaa tccagtgata atttgtgaat tcatagcatt ctttttacca tcgtgagtca
      481 aaatagttat tttttcctga ggtggttgtt taataaattg tgaggcaatc atcatgacaa
      541 aaaaatatac taatcccaaa atataaaacg ttttacccac accaatccta atcagtaagg
      601 attgtgcaag cggacttgtt actaaagaag caaagccaaa tcccataata gcgaatcctg
      661 ttgctagtcc cctcctatca ggaaaccatt taataatagt cgatactgga gtaatataac
      721 ctgaaccaag tccgattcct cctaaaatac cgtatgcaac atacagtaac cataactgct
      781 gagtttcaat ggctaagcct gttaacacat tccctgctcc atataaaata gcagaaatca
      841 ttcccattat cctaggacca aaacgctcta ctaagtgtcc cataaaagct gcagacattc
      901 ctagacaaaa aatagccaaa ctaaaagcga atgaaactga tgaaatatcc caaccagtct
      961 ctgagataat tgggttacga aacacactcc aagcataagt tgatcctagc attaaatgta
     1021 aaacgactcc agaaaccgca actacataac gatttaaatt tttcaaataa tcctcctaaa
     1081 aacgaactta agttttattt ttaaaaatat agttcgcaat aaacagatta tcaaaaaact
     1141 gaatatatca ccagttttct gcttagcata tctattttcc tcaataaaac tgaatattat
     1201 tcgtgattaa aaaacaaaaa agttagg
//
LOCUS       DATRQA010000248         1221 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.249, whole genome shotgun sequence.
ACCESSION   DATRQA010000248 DATRQA010000000
VERSION     DATRQA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.249"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..554)
                     /locus_tag="VBD27_002084"
     CDS             complement(<1..554)
                     /locus_tag="VBD27_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO7026047.1"
                     /translation="MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSN
                     ELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIII
                     GSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVM
                     ITGLSNMSIKRFVTIIMITKPISI"
     gene            647..996
                     /gene="ssrA"
                     /locus_tag="VBD27_002085"
     tmRNA           647..996
                     /gene="ssrA"
                     /locus_tag="VBD27_002085"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          413 a          216 c          191 g          401 t
ORIGIN      
        1 atgatagaaa taggtttcgt tatcataatg attgtcacaa agcgcttaat agacatatta
       61 gatagaccgg taatcatcac catgatgtcc gctggtgaaa tgggagatgc catacaaaag
      121 ataaaaaact tttcatatcc ggatgtttct agcttagact catatttatc aaatgtcttc
      181 tgatccataa atagcaaaac gaattttcgt ccataaaatt tgactaacca aaaaaggatg
      241 acacttccga tgataatccc aatatagtta taaataaagc ctaaggtagg accaaaaatt
      301 aagaaacctg ctactgttgt aacacctcca ggaattactg gaaaaacaat ctgtacaatt
      361 tgagccacaa taaaaatcat tggaccccaa aattcatact tgtgtactag gtcttttaat
      421 tcattgctat cattcaaaat accaagtttg tacaaataaa caactaagac aagggtcgct
      481 attaacgcta agacacccaa agccttaatg accttctgcc aaaagcggta acgttttgat
      541 aatttcatat tcatgcttta atcataacac aactgttaaa tttttgctat tttaaatagt
      601 atttgttata ctatattggt aagtttttat gctttattct acttttgggg tcgttacgga
      661 ttcgacaggc attatgaggt atattttgcg actcatcggc agatgtaaaa tgccagttaa
      721 atataactgc aaaaaataca aattcttacg cattagctgc ctaaaaaaca gcctgcgtga
      781 tcttcacaag attgtttgcg ttttgctaga aggtcttatt tatcagcaaa ctacgtttgg
      841 ctactgtcta gttagttaaa aagagattta tagactcgct atgtgagggc ttgagttatg
      901 tgtcatcacc tagttaaatc aatacataac ctatagttgt agacaaatat attagcagat
      961 gtttggacgt gggttcgact cccaccggct ccatatcatt ttatcataac ttactataca
     1021 tacttaaaac cttgttatta caaggttttt attttgtttc tttgctcata cattgcccct
     1081 atcttaggaa aaaaatactt ggaacagact ctaacattga attaggccac ctcattagct
     1141 cactctttgt caatatccga gaataagttc tttatcattc taatttatag aatttattaa
     1201 aaaagataag gtttttagag g
//
LOCUS       DATRQA010000249         1200 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.250, whole genome shotgun sequence.
ACCESSION   DATRQA010000249 DATRQA010000000
VERSION     DATRQA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.250"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            105..407
                     /locus_tag="VBD27_002086"
     CDS             105..407
                     /locus_tag="VBD27_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO7026048.1"
                     /translation="MTKKITVVALETLIAQHNNIHLIDVREEHEYRGGHIPGAINLPL
                     SQLSHKFEQLDKNKEYYLVCQRGGRSIRACQFLELKGYKVINVDGGTEAWRGNLEV"
     gene            complement(564..>1200)
                     /locus_tag="VBD27_002087"
     CDS             complement(564..>1200)
                     /locus_tag="VBD27_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7026049.1"
                     /translation="LFLLSIIYTTNNIVSTIWDAVLNHLNIMESESQNYDATFSILMI
                     SYSVVVGPFFEEVLYRGIVLNLLSKYGKWFAIITSGILFGLMHQDISQLLTTSIAGII
                     MGFIAYHYSFKVALLLHICNNFIVEIFTQLSTVNELYGTYFENILLILAILFILYYLF
                     THRNTARRRISLHSNVREADSINSKQHTKLLLTSWPFILLVIYDIVLTTIN"
BASE COUNT          440 a          166 c          229 g          365 t
ORIGIN      
        1 gatttacgac aatagtcata atagtagaac gatgtcacca ttttaaataa taaagtgatt
       61 agtcatttga ctaaatttgc caagtatcaa aggaaataaa gattatgact aaaaagataa
      121 ctgttgtagc attagaaaca ttgattgccc agcataataa tatccatttg atagacgttc
      181 gtgaagagca tgagtatcgt ggagggcata ttccaggtgc gataaatctt cctttgagtc
      241 aactcagtca taagtttgaa cagttagata aaaataagga atattatctt gtttgtcaac
      301 gagggggaag atctattaga gcatgccaat ttctcgagtt gaaaggctac aaagtaatca
      361 atgtagatgg tggcacagaa gcgtggagag gaaatctaga agtgtaaata tgtcctgtgt
      421 agtctaccat atttgataat aaaaaaattg acattatctc actattaatg accttttagt
      481 aaacataaag aaaagtatgt gttttgaaaa ggaggatgat tatccttttc aaaaccatac
      541 tttttgtgtc ttgtggcttg gtcttaatta atagttgtta ggacaatgtc gtaaatgaca
      601 aggagaataa aaggccaact agtaagtaat aattttgtgt gttgtttaga gtttattgag
      661 tcagcctctc gaacgttaga gtgcagactt atacgacggc gagcagtatt cctatgcgta
      721 aaaaggtaat ataatataaa taagattgct aaaattagca atatattttc aaaataagtc
      781 ccgtataact cgtttactgt tgatagctgg gtgaaaattt ctacgataaa gttattgcag
      841 atatgaagga gtagggctac cttaaaggaa taatggtaag cgataaaacc cattatgata
      901 cccgcaatag atgtagtcaa tagctgagaa atgtcttggt gcattaaacc aaataggatg
      961 ccagaagtga taatggcaaa ccatttacca tacttggata ataaattcaa tactattcct
     1021 cggtataaaa cttcttcaaa aaaaggacct acaacaacag aataactaat cataagtata
     1081 ctgaatgtgg catcataatt ttgactttcc gattccataa tattgagatg atttagcaca
     1141 gcatcccata ttgtggagac tatattatta gtagtatata taatgcttaa caagaataaa
//
LOCUS       DATRQA010000250         1182 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.251, whole genome shotgun sequence.
ACCESSION   DATRQA010000250 DATRQA010000000
VERSION     DATRQA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.251"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(56..>1182)
                     /gene="dnaN"
                     /locus_tag="VBD27_002088"
     CDS             complement(56..>1182)
                     /gene="dnaN"
                     /locus_tag="VBD27_002088"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO7026050.1"
                     /translation="SINKNFFLHALTVTKRAISHKNAIPILSTVKIEVTRDAIILTGS
                     NGQISIENTIPASNENAGLLVTNPGSILLEAGFFINIISSLPDVTLEFTEIEQHQIVL
                     TSGKSEITLKGKDVDQYPRLQEMTTDTPLTLETKLLKSIINETAFAASQQESRPILTG
                     VHLVISQNKYFKAVATDSHRMSQRTFQLEKSANNFDLVVPSKSLREFSAVFTDDIETV
                     EVFFSDSQMLFRSENISFYTRLLEGNYPDTDRLLTNQFETEIIFNTNALRHAMERAYL
                     ISNATQNGTVRLEIQNETVSAHVNSPEVGKVNEELDTVSLKGDSLNISFNPTYLIESL
                     KAVKSETVTIRFISPVRPFTLTPGEDTEDFIQLITPVRTN"
BASE COUNT          388 a          190 c          204 g          400 t
ORIGIN      
        1 tagcatatct tagaataaga aaactaccca agaataactt gggtagtttt cttatttagt
       61 tagtacgaac aggagttatt aattggatga aatcttcagt atcttcacca ggggtcaaag
      121 taaatggacg tactggagaa ataaatcgaa tcgtaactgt ttcgcttttt actgctttta
      181 aagattcaat taggtaagtt ggattaaaac taatatttaa actatcacct ttaaggctaa
      241 cagtatccaa ttcctcatta actttaccaa cttctggaga gtttacatga gctgagactg
      301 tttcattttg aatttctaaa cgaacagtac cgttctgagt tgcattcgaa attaaataag
      361 cacgttccat agcatggcgt aaagcatttg tattaaagat aatttcggtt tcaaactgat
      421 tagttaggag gcgatcagta tcagggtagt ttccttcaag caaacgtgta tagaagctga
      481 tattttcact tctaaataac atttgactat ctgagaagaa aacctctaca gtttcaatat
      541 catctgtaaa aacagccgaa aattctcgaa gggatttact tggaacaacc aaatcaaaat
      601 tattagccga tttctctaat tggaaagtgc gttgactcat acggtgtgaa tctgtcgcaa
      661 cagccttaaa gtatttattt tgactgataa ccaaatgaac acctgttaag attggacggc
      721 tttcttgttg gctagcagca aaagcagttt cattaataat tgattttaac agtttagttt
      781 ctaatgttaa tggagtatct gttgtcattt cctgcagacg agggtattga tcgacatcct
      841 tacctttcaa agtaatctct gattttccac tagtaagaac aatttgatgt tgttcaatct
      901 ctgtaaattc taaagttaca tctggtaaac ttgaaataat attaataaag aaaccagctt
      961 ctaacaaaat agagcctgga ttcgttacta gtaaacctgc attttcattt gaagcaggaa
     1021 tagtattttc aattgaaatt tgtccatttg accccgttaa aatgatagca tctctagtca
     1081 cttctatttt aacagttgaa aggattggaa tcgcattttt atgactaata gctcgtttgg
     1141 ttactgttag agcatgcaag aagaaatttt tatttattga aa
//
LOCUS       DATRQA010000251         1158 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.252, whole genome shotgun sequence.
ACCESSION   DATRQA010000251 DATRQA010000000
VERSION     DATRQA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.252"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            459..851
                     /locus_tag="VBD27_002089"
     CDS             459..851
                     /locus_tag="VBD27_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEO7026051.1"
                     /translation="MQSQKLNILQRVLRDKFLLQGLGVPNSNTVLNRSFTALLLALLL
                     EDSNNKSWISEEDQIKIMNQAFSWLIDELDFRGFDEELGWIHVFAHGADLLTEIVKMK
                     CFSKDDSSKILTIIKRVMITDDILTLNG"
     gene            1030..>1158
                     /locus_tag="VBD27_002090"
     CDS             1030..>1158
                     /locus_tag="VBD27_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEO7026052.1"
                     /translation="MRDEQEIYNLVLNIANQDKRIEAVLLNGSRANPNVPKDDFQDY"
BASE COUNT          445 a          131 c          211 g          371 t
ORIGIN      
        1 aggaaattat aaattaagca gaagtatttt agatttttat acttttatac tttctatatt
       61 ctctggtaat tttagttcta tcgtatattt atggtatgcc tcaatttttt cctaatagag
      121 gttacaaggt aggccttcgc tatatttcta gagtcaaaga ataagtacta aaaatttctg
      181 atataaaagt gagtatccaa gtaaaaaatt aaagttacta taatcatttt aaagtgaaaa
      241 cgaaaactct aatgttacac tttggaaaat ataaaatata aagtcctgca aggagaaaca
      301 tggaagcaag tgatgtaatc aaaaagatag aaaataatta tccaattgat gagaatgttt
      361 tatctttttg attacatcat ataggagatt ggaacccgag agttcgagat gaaatagtat
      421 atgggtcttg ggttaaatta gtaatagaac ataaactgat gcaaagccag aaattaaata
      481 tccttcagag agttttaagg gataaatttc ttttacaagg attaggagta cccaacagta
      541 atactgtgtt aaacagatct tttactgctc tattactagc tttattgttg gaggactcaa
      601 ataataaaag ttggatatct gaagaagatc agataaaaat tatgaatcag gcattctcct
      661 ggcttataga tgaacttgat tttagaggct ttgacgaaga gcttggatgg atacatgtct
      721 ttgctcatgg agctgattta ttaacagaaa tagtcaagat gaaatgtttt tcaaaagatg
      781 acagttcaaa aatcttaact atcataaaaa gagtgatgat aactgatgac atactaacat
      841 taaacggttg aaaatatttt gaatcataat cagttgttca gaaaaattta aaattgaatc
      901 cttggaggtc gtaaaggtat ttccatgcta atttaagttt atgattgcat gcttagagaa
      961 aaatacaagg gtaatataaa ggtattagaa aaaatatggt aatataaagt gaagtaggag
     1021 gtagccatta tgagagatga gcaagaaata tataatttgg ttttaaacat agcaaatcaa
     1081 gataagagaa tcgaagctgt actattgaat ggatctcggg ctaacccaaa tgtaccgaaa
     1141 gatgattttc aagattat
//
LOCUS       DATRQA010000252         1150 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.253, whole genome shotgun sequence.
ACCESSION   DATRQA010000252 DATRQA010000000
VERSION     DATRQA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.253"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            43..573
                     /locus_tag="VBD27_002091"
     CDS             43..573
                     /locus_tag="VBD27_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026053.1"
                     /translation="MSPSEKLDRRIDSSSDLSQYVYDFEKQYTEYMKKGYSEKNASIQ
                     ARRTLNEILQENNPGLRSSDAMYDKETGIAAIAVKDKNTGETYIAYAGTNKDADGATD
                     FISDANIGANNSAYLKRLGDKATSFYDRVQSNGDNITVTTGHSYGDFLASRVAIELIP
                     FFINFRKVNDSFHFCY"
     gene            665..888
                     /locus_tag="VBD27_002092"
                     /pseudo
     CDS             665..888
                     /locus_tag="VBD27_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          412 a          163 c          217 g          358 t
ORIGIN      
        1 attcgaaggt attacattag aggatgtaaa ggagttttct aaatgagtcc aagtgagaaa
       61 ttagatagaa gaattgattc ttcatcagac ctctcccagt atgtatatga ttttgaaaag
      121 caatatacag aatatatgaa gaaggggtat tctgaaaaaa atgcgagtat acaagcacga
      181 cgaaccctga atgaaatact acaagaaaat aatccaggat tgagatctag cgacgccatg
      241 tatgataaag aaacaggtat tgcagctatt gctgtaaaag ataaaaatac tggtgagact
      301 tatatagctt atgcgggtac taataaggat gctgatgggg caaccgactt tatttcagat
      361 gcaaatatcg gtgctaataa ctctgcttat ttaaaacgat taggagacaa agctactagc
      421 ttttatgatc gtgttcaaag taatggcgat aatattactg tcacaacagg tcattcttat
      481 ggtgactttc tcgcttctag agtggcaatt gagctaattc cattctttat taactttaga
      541 aaagttaatg attcatttca cttttgttat tagcttgtga tcacttataa agtgacagct
      601 agttcatgaa atttcttagg agttttctgg ctttaaaggt aacctatgat ttaaaaaaca
      661 tctcatggaa ttcaatgcgg aatcctcaac agtcttttgt ggactaacag aagttcagga
      721 tgtcgtttcg gcaggcctta actattctca gaaggttttg gaaatttcaa tggtacattt
      781 gatacctcag gtaagggatt agaatggaca aaaacaattc aaacaggtat gaaaaaacga
      841 gaagagaaaa tcaaataccc tcattttaca attgttgaca taaagtaatt aaacaaaatc
      901 cggaaaattt atattaagta atttttctaa aaagtcatat aggcatactt ttaaagttcc
      961 tctaaactta ataaggaaga aacgttttga aaaaagataa atatattgca ttgaattggt
     1021 tgtgtgcgat agccacgcta tactattaag ttattacaaa ctatgaatta cacctcaaat
     1081 gttggagata aaacctatcg tttggggtgt tttttggtaa aatagaatga aaagagttac
     1141 tctgtgtcat
//
LOCUS       DATRQA010000253         1145 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.254, whole genome shotgun sequence.
ACCESSION   DATRQA010000253 DATRQA010000000
VERSION     DATRQA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.254"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            120..1043
                     /locus_tag="VBD27_002093"
     CDS             120..1043
                     /locus_tag="VBD27_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7026054.1"
                     /translation="MRIQQLQYVIKIVETGSMNEAAKQLYITQPSLSNAVRNLETEMG
                     IQIFIRNPKGITLTKDGMEFLSYARQILEQTALLEERYKGDNTSRELFSVSSQHYAFV
                     VNAFVALFNGTDMTQYELFLRETRTWEIIDDVKNFRSEIGVLFLNSYNRDVLTKLFDD
                     NSLIATTLFTTTPHIFVSKSNPLANRKKLSMKDLEDYPYLSYDQGLHNSFYFSEEMMS
                     QIPHPKSIVVSDRATLFNLMIGLDGYTVATGILNSKLNGDEIVAIPLDVDDVIDIVYI
                     RHDKANLSKMGQKFIDYLLEEVSFNHKNKQS"
BASE COUNT          381 a          188 c          191 g          385 t
ORIGIN      
        1 aacgaaaata atattggaat aatctgatta tattagctaa ataacatttt caattgatct
       61 atattgataa tttagctgat ctaattgaat ttatttacat tctagtttaa actagaatta
      121 tgagaattca acaattacaa tatgttatta agattgtaga aactggtagc atgaatgaag
      181 ccgctaaaca actctacatt acccaacctt cactttcaaa cgctgttagg aaccttgaga
      241 ctgagatggg tatccaaatt tttattcgaa atccaaaagg aataacgtta acaaaagatg
      301 gtatggaatt tctgtcatat gcgcgacaaa ttctagaaca aacagcactt cttgaagagc
      361 gttataaggg tgataataca agtcgtgagc tgttcagcgt ttcttcacaa cactatgcat
      421 ttgtggttaa cgcttttgta gctctcttta atggcacaga catgacccaa tacgaacttt
      481 ttttacgtga aactaggact tgggaaatta ttgatgatgt taaaaatttc cgttcagaga
      541 ttggtgttct gtttcttaat agctataata gagatgtatt aaccaaatta ttcgacgata
      601 attccttaat tgccacaact ctttttacta ctacacctca tattttcgtg agcaaatcca
      661 atcccttagc taatagaaaa aaattaagta tgaaagattt agaagattat ccttatctga
      721 gttatgatca aggcctgcat aattctttct acttttctga ggaaatgatg tctcaaattc
      781 ctcacccaaa atccattgtt gtcagtgata gagcaactct gtttaattta atgattggat
      841 tagatggtta tacagtggca actggtatcc tcaactcaaa attgaacggt gacgaaattg
      901 tcgcaatccc tcttgacgtg gatgatgtta ttgatattgt ctatatccga cacgacaaag
      961 caaacctttc aaaaatggga caaaaattta ttgactattt attagaggaa gtttcgttta
     1021 atcataaaaa taaacagtcc tgagattgtt gtactgaccc ccaaaagttg gacaatttat
     1081 tttaagtgaa ggatttagtt ctgtattgca caggactaag tccttttagt tttgctttga
     1141 ttcgc
//
LOCUS       DATRQA010000254         1120 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.255, whole genome shotgun sequence.
ACCESSION   DATRQA010000254 DATRQA010000000
VERSION     DATRQA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.255"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(442..660)
                     /locus_tag="VBD27_002094"
                     /pseudo
     CDS             complement(442..660)
                     /locus_tag="VBD27_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001889697.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          352 a          193 c          143 g          432 t
ORIGIN      
        1 tattattttt tacatattta ttaattaata tggtaacacc cattatctga agtgtgtagg
       61 cgaggtctaa gtaaagaatt taagcgtttt gataataaaa aagtgagaac aatagattct
      121 cactcacaat tagcctggta atttctcaca cgatttggta ctgctttttt taagttgggc
      181 gatggtgtta tttttaatat gagtagctgc actcgaatcc gattgctcaa aagcacttgt
      241 cgctccattg agatagctat tcagatcaga aataactggt tgaatgtttt ggctgacttc
      301 tttgataact tttgatccag cttctaagta ttttcaatgt tcttgaaccc attggtgatc
      361 tttttttact aagtcacatg ctgttaaaat catcattaag cttatccctt ccacaataat
      421 aatcaatttt ttaaatcctt tttatcttat tgattttttc aattttattg ctgtcttgac
      481 gttgctatcg caaacaacca actcaatgca atatagttac ggtatacata tcaatgtaag
      541 actcaagatt tttaaattca tattatttgt aggttcttta tcagaccaag ctaatactct
      601 ttcatttgaa gtaatattat tatttgtatg atattcaaga ttagctattt ttgatttcac
      661 aaaaaaacct tcttctactt ttttagactg attctttctt cccacttctt cgtatcaata
      721 tcatatattt gaagaactaa actctcagag tttccagtca attttagtaa taatcccaaa
      781 actaatcctt cctacaaacc aacggataat tttatttcaa ctggtcctgg ttttaatttt
      841 acttgtgcaa tttcgcttgt tataccaact attgatatcg ccgatactcc tattcctaat
      901 gatgcgcacg catcattagt taaacaaagt actttaaatc ctacagaggg tcattccatc
      961 aattaatggc ataggttttg gcactagaaa ctgaatatga attctattat ctattaaaaa
     1021 aatcaatatc accagagtat ttataaaaca taacgataaa aaatcaaaaa atatttttac
     1081 tatttccctt aatttcatga gatttaattt ccctttttgt
//
LOCUS       DATRQA010000255         1112 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.256, whole genome shotgun sequence.
ACCESSION   DATRQA010000255 DATRQA010000000
VERSION     DATRQA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.256"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(1..284)
                     /locus_tag="VBD27_002095"
                     /pseudo
     CDS             complement(1..284)
                     /locus_tag="VBD27_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            462..662
                     /locus_tag="VBD27_002096"
     CDS             462..662
                     /locus_tag="VBD27_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7026055.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFIEKTIAKGK
                     ELADDAKDAVEGAVDAVKEKLK"
     gene            704..892
                     /locus_tag="VBD27_002097"
     CDS             704..892
                     /locus_tag="VBD27_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO7026056.1"
                     /translation="MSIRTDIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLLHEEVR
                     EEEVVTVERVLDVLRKLS"
BASE COUNT          407 a          140 c          220 g          345 t
ORIGIN      
        1 tttgcatcca tacgatgact caatttccaa gcaatatatt ttctagaata gagatcaatt
       61 attgcgcaga gatagacata tttcttatat ccaatagaaa tataggtgaa gtcagtagac
      121 catacttgat taggtttatc aggataaaat ttctggttga gtaagttttg aggatacgtt
      181 ttttgagtct ttttaagagc tgtttttgtc ttaacggtag ccattttagg gagcgccata
      241 ttcttcatca gacggtaaat tcttccttca gagatttttg tgtcttttaa aacaggttat
      301 aacacacaaa gtgttgtcta tttttatagt atacatccat actcgcataa ccatgagata
      361 atcactaaaa caagagtcgc ctgacactag cgactcttgt ttttatttgc taaagaaaga
      421 tatagatgtt atgattaata aaaataataa ggaggtgcag tatgtcacaa gaaaaactaa
      481 aatcaaaatt agatcaagca aaaggtggtg ctaaagaagg ctttggcaaa ataaccggtg
      541 ataaagagtt agaagcaaaa ggatttatcg aaaaaacaat tgctaaaggc aaagaactag
      601 cagatgatgc taaagatgct gttgaagggg cagtagatgc tgtcaaagaa aaactgaagt
      661 aacaaataac cgctcattta acgagcggtt atttgttatc tttttgtcta tcagaacaga
      721 tatagatgag tttaaagaag cgattgataa aggctatatt tcagggaaca cagtagcgat
      781 agtgcgtaaa aacggaaaga tatttgatta tgtgttacta cacgaagaag tgagagaaga
      841 agaggttgtt acagttgaga gagtgcttga tgtactgagg aagttatcat aataacggac
      901 caaaattcgc cccaaaaagt attttaaaat agaataaaat aaaaaaatat gctttttatt
      961 atggatgtat cgtgtatttt aacctttaat atatcctatt ttaatggtag agaagttggc
     1021 aggggacata ttaaaacctt ttttgagatg ttaggaagga ttttattctt tagactagtg
     1081 ttgagaaaat aacaaaactt aaataagatt at
//
LOCUS       DATRQA010000256         1110 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.257, whole genome shotgun sequence.
ACCESSION   DATRQA010000256 DATRQA010000000
VERSION     DATRQA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.257"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            67..1053
                     /gene="dhaQ"
                     /locus_tag="VBD27_002098"
     CDS             67..1053
                     /gene="dhaQ"
                     /locus_tag="VBD27_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEO7026057.1"
                     /translation="MRKTEHHKMEYVFETIKAITKTNPKVEQIKDLPAIHYLGQSEQV
                     IPILSGGGSGHEPAHFGYVGEGMLSAAISGPIFVPPCASDILETIRFINRGKGVFVII
                     KNFEADLEEFSQAIEQARQEGIPIKYIVSHDDISVETSNFKIRHRGVAGTVLLHKIIG
                     QAALEGASLDELEQLGLSLTTSMATLGVASKSATILGQHQPVFDLEEGYISFGIGIHG
                     EPGYRTMPFVSMEHLANELVNKLKMKLRWQDGEAFILLINNLGGSSKMEELLFTNAVM
                     EFLALDDLQLPFIKTGHLITSLDMAGLSVTLCRVKDSRWIDYLKHKTDARAW"
BASE COUNT          380 a          163 c          232 g          335 t
ORIGIN      
        1 ttatttaata ggaaagtcat tgaaaaaatt atcagaacat caatagaaaa ttattaggac
       61 aaggatatga gaaaaacaga acatcataaa atggaatatg tttttgaaac aataaaagca
      121 atcactaaaa ctaatcctaa agtagagcaa ataaaagatt tgcccgctat tcattattta
      181 gggcaatccg agcaagtaat tccgatttta tcaggaggag gcagcggaca tgagccggct
      241 cactttggat atgttggtga gggaatgcta tcggcagcga ttagtggtcc tatttttgta
      301 ccaccatgtg ctagtgatat tctagagact attagattta tcaatcgagg caaaggtgtc
      361 tttgttatta tcaaaaattt tgaagctgat ttggaagaat tttcccaagc gattgaacag
      421 gctcgtcaag aaggcattcc aatcaaatac atcgtttctc atgatgacat ctctgttgaa
      481 acatcaaatt ttaaaattcg tcatcgaggc gttgctggta ctgtgttgct acataaaatt
      541 attggacaag ctgctttaga aggagcaagc ttagatgaat tagaacagct aggactatct
      601 ttgacaactt caatggctac tttaggtgta gcaagtaagt cagccactat tttaggtcag
      661 catcaacctg tttttgattt agaagaaggt tacatctcat ttggaatcgg tattcatggt
      721 gagccaggat accggacaat gcctttcgtg tccatggaac atttggcaaa cgaactagtt
      781 aacaaattga aaatgaaatt acgttggcaa gatggagaag cattcattct attgataaat
      841 aatcttggag gaagttctaa aatggaggaa ttacttttta caaatgctgt catggaattt
      901 ctggctttag acgatttaca attacctttt attaaaacag gccacctaat tacaagttta
      961 gatatggctg gtttatcagt taccttgtgt cgtgtaaagg atagtagatg gattgattac
     1021 ttaaaacata agacagatgc tagagcttgg tgatagaagt aagtggtaag agtttttaga
     1081 atagaaaata ctttaaggag aacagagaaa
//
LOCUS       DATRQA010000257         1088 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.258, whole genome shotgun sequence.
ACCESSION   DATRQA010000257 DATRQA010000000
VERSION     DATRQA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.258"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..297
                     /locus_tag="VBD27_002099"
     CDS             <1..297
                     /locus_tag="VBD27_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7026058.1"
                     /translation="KQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDSVT
                     AGEIINILKELAQDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
     gene            297..>1088
                     /locus_tag="VBD27_002100"
     CDS             297..>1088
                     /locus_tag="VBD27_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7026059.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMATLSIISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTNSIDSYVKRINSVADLV
                     DHDIIETQDTLANQSPERAKNFKRTVMLTGVNDSAKETKFVSEAYKLVEGKHLENKDK
                     NKILMHKDLAKKNNLKVGDKIKIKSNLFDADNEKVANETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHSAAKVYGNTEDTAVYQDATFFVKGDKNLDSVI"
BASE COUNT          463 a          131 c          202 g          292 t
ORIGIN      
        1 aaacaaataa aaagaaatgt tatgaaatta tctggtggtc agcaacaaag ggtagctatt
       61 gctagggcac tggtatcaga tgccccaata atactagctg atgagcctac cggtaaccta
      121 gacagtgtta ctgctggaga aataattaat atattaaagg aattagctca agatagaaat
      181 aaatgtgtaa tagttgtaac acatagtaag gaagtagcag attctgcgga tatcatttta
      241 gaactaagtg gtaaaaagtt gaaaaaagta aataaaatga atttggaggt tgaataatga
      301 taaaaaatgc atttgcttat gtaactagaa aaagcttaaa atcattaatt attatattag
      361 ttattctatc aatggcaact ttaagcatca ttagtttatc tattaaagat gccacagata
      421 gagcttctaa ggaaacattt gctaatataa caaacagttt ttctatggaa ataaatagac
      481 aagtaaatcc aggaacacct aggggtggag gaaatgtaaa aggtgaagat attaaaaaga
      541 tatctcaaac aaacagtata gactcctatg taaaaagaat aaacagcgtc gcagacctag
      601 ttgatcatga tataattgaa actcaagata cacttgcaaa tcaatcacct gaaagagcaa
      661 agaattttaa gagaacagtt atgttaacag gggttaatga ctcagcaaaa gaaacaaaat
      721 ttgtatcaga agcatataag ttagtagaag gaaagcattt agaaaataaa gataaaaata
      781 aaattttaat gcataaagat ttggcaaaaa aaaacaatct caaagttgga gataaaataa
      841 aaataaaatc taatttgttt gatgctgaca atgaaaaagt tgcaaatgaa actgtagaag
      901 tagaaattaa aggtttattt gatggtcata atagcggtgg ggtaagtgca gcacaagaac
      961 tatacgaaaa tacactaatc actgacgttc attcagctgc taaagtttat ggtaatacag
     1021 aagatacagc cgtataccaa gatgcaacat tctttgtaaa aggtgataag aatcttgata
     1081 gtgtaata
//
LOCUS       DATRQA010000258         1071 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.259, whole genome shotgun sequence.
ACCESSION   DATRQA010000258 DATRQA010000000
VERSION     DATRQA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.259"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            133..1038
                     /locus_tag="VBD27_002101"
     CDS             133..1038
                     /locus_tag="VBD27_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7026060.1"
                     /translation="MKNQQKLMYLESIELYSNITKAAAHLFISQPYLSKVIKQLENEL
                     EIKLIQSQGHQTFLTYAGQRYLFYLKEIDMIERQMAKELYLIRSDKKGEITLGINSGL
                     ASSILANVLPKFNLEHPEISVKLLENNQNISEQLVASGDIDLAVGMAPILYKDGIAST
                     TIYRDELFLMIPTTSQLYNAEKRGQIIPFEYPISVLDNEPLILTPLEYGIGKTIAQFY
                     ELHHMSLNQMITTSTVPTAASLSLSGMGATFVPQTLIHRYLDKECNVYHFHKNKLFSE
                     YIMIYKKDVELSGIALLLYKAFLTK"
BASE COUNT          375 a          202 c          159 g          335 t
ORIGIN      
        1 atagttatga atatatcatg ttatactact aaattaaata gattgatttt agctcaaaat
       61 gatagtaaac ttatagccat aacgtatcat aacttattat taatctaagg atatcaaact
      121 ggttataaag tcttgaaaaa tcaacaaaaa ttaatgtatc tagagagtat cgagctatac
      181 tcaaacatca ctaaagccgc tgcacacctt tttatttctc aaccttacct aagcaaagtg
      241 atcaagcaat tagaaaatga gttagaaata aaactcatcc agagccaagg acaccaaacc
      301 tttttaactt atgctggaca gagatatcta ttctacctaa aagaaattga tatgattgag
      361 cgtcaaatgg ccaaagaact atatctcatc cgttctgata aaaaaggaga aatcacactt
      421 gggattaact ctggcttagc aagttctatt ctggcaaatg tacttcccaa atttaacttg
      481 gagcacccag aaatttctgt caaattactc gaaaacaatc aaaatatttc tgaacaattg
      541 gtagcgagtg gtgatattga cctagctgtt gggatggctc ctatccttta taaagatgga
      601 attgcatcaa ccactattta ccgcgacgaa ttgtttttaa tgatacctac caccagccaa
      661 ctctacaacg ctgaaaaacg tggacagatt attccttttg aatatcctat ttcagttcta
      721 gacaatgaac ctcttatcct aacaccatta gagtacggaa ttggcaaaac aattgcacaa
      781 ttctatgagc ttcatcacat gtcattaaat cagatgatta caactagtac cgttcctaca
      841 gcagctagcc tatctttgtc agggatggga gcaacatttg ttccacaaac tctcattcat
      901 cgctatttgg ataaagagtg caatgtctac cattttcata agaataagtt attttcagag
      961 tacattatga tttataaaaa agatgttgaa ttatctggaa ttgctctcct actttataaa
     1021 gcttttttaa ccaagtagtt tcgctaattc tattgaaaat cagtttactt c
//
LOCUS       DATRQA010000259         1063 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.260, whole genome shotgun sequence.
ACCESSION   DATRQA010000259 DATRQA010000000
VERSION     DATRQA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.260"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..965)
                     /locus_tag="VBD27_002102"
     CDS             complement(<1..965)
                     /locus_tag="VBD27_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO7026061.1"
                     /translation="MSEKIRVLLYYKYVSIENAEEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWIHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENEAKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGES"
BASE COUNT          338 a          250 c          142 g          333 t
ORIGIN      
        1 aaggattctc caatagcttc taaacgttct gcccattctt gacgtgataa accattttct
       61 tgcacatagc gattacgttc atgtgcacga cattcaggcg agcaccctcg aacatacttc
      121 gcttcgtttt cttctgaagc aaaaatttgt ttgttacaaa acggatttgc acaattgaca
      181 taacgttcac aaggtgttcc atcaaaataa tctttactaa taacagttgg attaacatga
      241 ttaattggca ccgagatacg atcatcaaaa acatacatcg caccatccca aagttcacct
      301 tgaacttccg gatcttttcc atatgttgca ataccaccgt gtaattgtcc aacatcttta
      361 aaaccttctc gaaccatcca acccgagaat ttctcacaac gaacaccacc ggtacagtac
      421 acaacaacac gtttttccat aaacttatct ttgttgtcac gtacccattg tggtaactca
      481 cggaaattgc gaatatcagg acgaattgct ccacggaagt gacctaagtc atactcataa
      541 tcattacgcg tatctaaaac aacagtatct tcatcaagca aggcttcttt aaattgttta
      601 ggatttaaat actcacctgt tgtttccaaa ggattaatat cgctatcaaa gttattatct
      661 tctaacccaa ggtgcacaat ctctttctta taacgaacaa acattttacg gaaagcttgt
      721 tgattttcct cgtcaatctt gaaccaaaga tcagcaaaac gctcatcgct gtggatccaa
      781 tccatatact tctgagttgt ttcataatct ccagaaacag ttccattaat cccctcatcc
      841 gcaatcaaaa tacgaccttt aagcccaata gatttacaaa attctagatg tttcgcagcg
      901 tactcttccg cattttcaat tgaaacatat ttataataga gtaaaactct gattttttct
      961 gacatttttt ctcctcatct gcaaaataag ttagtatact tcattatata acgactcctt
     1021 acaacatagc aaaaaatacg cttaggacat attctttgct tta
//
LOCUS       DATRQA010000260         1047 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.261, whole genome shotgun sequence.
ACCESSION   DATRQA010000260 DATRQA010000000
VERSION     DATRQA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.261"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(94..351)
                     /locus_tag="VBD27_002103"
     CDS             complement(94..351)
                     /locus_tag="VBD27_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HEO7026062.1"
                     /translation="MTQHLLNCYPEYFEAIMDGTKTFECRYNDRDFKVGDELLLREYD
                     PQKGYTYRCIVRKITYILSDFIGLKDGYVILGVTNEPIDYF"
     gene            complement(535..906)
                     /locus_tag="VBD27_002104"
     CDS             complement(535..906)
                     /locus_tag="VBD27_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026063.1"
                     /translation="MSDNIEIIMDIETLDIFEVIDLDEEKEVAVSDSETFPKEEKVSP
                     YDDLNRIWLRNGYYYKPSSLLWEVVRDGIIHELSPRNKHGTYPCCEHSRNGTWGHVKQ
                     LTQEGALWLTLELNPDLMNYV"
BASE COUNT          325 a          211 c          154 g          357 t
ORIGIN      
        1 tttatgacaa agagaaataa gttcaataaa taaggctctc tcttgtgata gttactgata
       61 aacatggggt agaagacaaa agcaaaacta atgttaaaaa taatcaatgg gctcatttgt
      121 cactcctaaa attacataac catcttttaa accaataaag tccgatagaa tataagttat
      181 ttttctaaca atacagcgat aggtgtatcc tttttgggga tcatattctc tcagtaacag
      241 ttcatcgcca actttaaaat ctctatcgtt atagcgacat tcaaatgtct ttgtcccatc
      301 cataatggcc tcaaaatact ctggataaca gttcagcagg tgttgtgtca cttcttttcc
      361 tcctctgcag cttcaataag ctgaagttca ataagcttat tactgggttg caaaagtata
      421 catctcttac actcttgctt tgtgtgcttt actttccaca atggaatcac accaaataga
      481 tatagccgtg cagtataaag aatctcatcg tctctactct tcacaatgaa tctcctacac
      541 ataattcatc aagtctggat tcagttccaa agtaagccat aaagctcctt cttgtgtgag
      601 ttgcttgaca tgaccccaag tgccatttct ggaatgttca cagcagggat atgttccgtg
      661 tttatttcga gggcttaact catggatgat gccatctctt acaacttccc atagaaggct
      721 tgaaggtttg taataataac catttcttaa ccaaattctg tttagatcat catatggaga
      781 tactttctct tctttaggaa aagtttcaga atctgaaaca gccacttctt tctcttcatc
      841 aagatctatc acttcaaata tgtctagagt ttcaatatcc atgatgattt caatattatc
      901 tgacattgcc gtatatcctt tcactagttt tgaccaacga aaaaagagaa cgattttctc
      961 gttctcttaa gctataagga tatttaattt tgtttttccc gaaactctag acacttacaa
     1021 gccattaatt tcattatatt ttaaatt
//
LOCUS       DATRQA010000261         1034 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.262, whole genome shotgun sequence.
ACCESSION   DATRQA010000261 DATRQA010000000
VERSION     DATRQA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.262"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(3..563)
                     /locus_tag="VBD27_002105"
     CDS             complement(3..563)
                     /locus_tag="VBD27_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7026064.1"
                     /translation="MNKFFLESERLIIRPLEESDYDSWYTSFMNRGPSLTPFDDGILD
                     MSMCDINWFKNLVNLQRKEWESDIVYIFGVFLKSGEHVGMLNIVTLARADMQWGELGY
                     VFHNQFWSNGYAFESILALLNSTYEKLGFHHIEAQITPGNERSEKLVRRLGLTYETTR
                     KDFSFENGKWTDKLIYSINLHNNNLE"
BASE COUNT          363 a          180 c          117 g          374 t
ORIGIN      
        1 aattattcta aattattatt atgtaaattt atactataaa tcagtttatc tgtccatttt
       61 ccattctcaa atgaaaaatc ttttcgagtt gtctcatagg ttaatcccaa tcttctaacc
      121 aacttttccg atcgttcatt cccaggagta atctgtgctt caatatgatg aaaacccaat
      181 ttttcataag tactattaag taatgccaag attgactcaa atgcatagcc attactccaa
      241 aattgattat gaaaaacata tcccaattct ccccattgca tatctgctct tgcaagggta
      301 acaatattta acattcctac gtgctcaccg ctttttagaa aaacaccaaa tatatatact
      361 atgtcacttt cccattcttt cctttgtaaa tttactaaat ttttaaacca attaatgtca
      421 cacatagaca tatcaagtat cccatcatca aatggagtta aagatggtcc acgattcata
      481 aaactagtat accaactatc ataatcactt tcttctagtg gccttataat aagtctctct
      541 gattctaaaa aaaatttatt caatttttat ttcctttctt ccagagtaaa taaataagtg
      601 tgttttaatt aaaatttcca taatattacc tccttgaaaa gttattaact acattataaa
      661 aaatacaata ataaaaaaca aatataagta attttaggta acttttcaca ataaattatc
      721 taacaaaaca aaaaagacaa ggtctagaaa ccttatcttt taaagtatac cggcggctgg
      781 ggtcgaaccg acacgtccgt gaggacactg gattttgagt gctactgagc actttttaaa
      841 gataacaaac attgatttaa caaggtttga gtattagaca ttaacttaaa aaccaagtaa
      901 ttttgagatt ttagtgattt tgtagtgact gttcgtcgtg aaaaataaat actcatttta
      961 ctgcaagatt ctttattttt catatttata tatattactt gaaaaaattt ttatcctttt
     1021 tttgcttcgt tttg
//
LOCUS       DATRQA010000262         1017 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.263, whole genome shotgun sequence.
ACCESSION   DATRQA010000262 DATRQA010000000
VERSION     DATRQA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.263"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            122..403
                     /locus_tag="VBD27_002106"
     CDS             122..403
                     /locus_tag="VBD27_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026065.1"
                     /translation="MSLIGAVLKGTKEDKEFKKQIQKALVRFVHAPEIVEEADSIKTT
                     YSEKIDDISETAKSSIKTIKNQIDTGLVGQFWEKVKAQVDLNKGSYDDL"
BASE COUNT          414 a          126 c          171 g          306 t
ORIGIN      
        1 agaggaaaat agtcggacac agtttctaaa agaccgagta aaattagaaa atgacatttc
       61 tagtttagaa ggaagaaaat actactaatt tagatggact ataaagtgaa agaggcaata
      121 aatgagttta ataggcgctg tattgaaagg aaccaaagaa gacaaagaat tcaaaaagca
      181 aattcaaaaa gctttagtaa ggtttgtcca tgccccggaa atagtagaag aagctgattc
      241 tattaagacg acttatagtg aaaagattga tgatatttct gagactgcta aatcatcaat
      301 aaaaacgata aaaaaccaaa ttgacactgg acttgtagga caattttggg aaaaagtaaa
      361 ggcacaggta gatttaaata aaggtagtta tgatgattta taatcgagtg aaaatgttga
      421 ggaagcttac tatcctgata gttattatag atatgaaaat aatcaagttg gattagaaga
      481 cataacattt tacatgaagg ataacaaagt ttattattta cattttggta aagcaataga
      541 taaaaatgat aagtatctca atcaaattat aaataattaa tttctttatt ttataagccc
      601 cgaaaacaaa ttttttgata accttttgat aacccctccg cgaacatatt gtaatacata
      661 gtgcactttt gggatataaa ttgttgattt ttagctagtt tataccatat aacaccatat
      721 attacagttg tcagagaaaa ccaggtttaa aaaaagcccg taaagcatca caatcctcaa
      781 aacgttaata tatacctata tatcagcgat tggaagcact tcatggttca caccatgggg
      841 tgttttttgt gtaatattca aaaattgtcc taaagtcatt tttaaaactt ctaaatactt
      901 gagaaaccat tgtaaattta aataactact ttaaatagca caaaggcaat gacaaagtta
      961 gatgagatga ttctatttta atttataatt gaaaaaaaga gtatatgatg gaggaat
//
LOCUS       DATRQA010000263         1013 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.264, whole genome shotgun sequence.
ACCESSION   DATRQA010000263 DATRQA010000000
VERSION     DATRQA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.264"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(165..>1013)
                     /locus_tag="VBD27_002107"
     CDS             complement(165..>1013)
                     /locus_tag="VBD27_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO7026066.1"
                     /translation="DFCGDETELSVRQLARIESGQSIPNLTKAHYIAKQLNVKLDILT
                     GGESLELPKRYKELKYLILRIPTYADAERLKLRECQFDHIFEEFYDNLPEDECLAIDS
                     LQAKFEVYQTGDINFGVEVLCECFDKVKYKEKYTLNDLIIIDLFLTCAVVSKFNNRAF
                     TKEVFQTICKTLISQNHKLTAEDLFWFNHVLLNCVFVGLCLNSEECLAEMLEVSRQTM
                     VSTHDFHKMPLYFMYQWKYFITIDNDIKSAENAYQQSIMFSKMIDDKHLIKKLELEWQ
                     EDITGH"
BASE COUNT          347 a          184 c          115 g          367 t
ORIGIN      
        1 gcccatattc caccaaccca ttcctaaaaa caaatcaaat gaacctaaat ttgataatag
       61 aacaagtaat actattaaaa aacatttttt aatcactata ataaactcct aaataatatt
      121 ttgtacgttt attataaatt tttagacctc agagtcacag gacatcaatg tcctgttata
      181 tcttcttgcc actccaattc taatttttta attagatgct tgtcatcaat catttttgaa
      241 aacataattg attgttggta agcattttct gcagacttta tatcattgtc aattgtaatg
      301 aagtatttcc attggtacat aaaatataaa ggcattttat gaaaatcatg tgttgacacc
      361 atcgtttgtc ggctaacttc taacatttct gcaagacact cttcactatt taaacaaagt
      421 ccaacaaata cacaattcag gagtacatga ttaaaccaaa acaaatcttc tgctgtaagt
      481 ttgtggttct gacttatcaa agttttacat atagtttgaa atacttcttt agtaaatgct
      541 ctattgttaa atttagatac aactgcacaa gttaaaaaca aatctatgat aatcaaatca
      601 ttaagagtat atttttcttt atattttact ttatcaaagc attcgcataa aacctccaca
      661 ccaaaattaa tgtctcctgt ttgatagact tcaaatttag cttgtaaact gtcaattgct
      721 aagcactcgt cttctggaag attatcatag aattcttcga aaatatgatc aaactgacat
      781 tctcttaatt ttaatcgttc agcatctgca taagttggaa ttcttaaaat caaatacttc
      841 aactctttat accgtttagg taattctaag ctctctcctc ctgttaaaat atctaatttt
      901 acattcaatt gtttagctat gtagtgagct ttagtcaagt ttgggattga ctgtccactt
      961 tcaatcctag ctaattgtct tacagataac tcagtttcat caccacaaaa atc
//
LOCUS       DATRQA010000264         1002 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.265, whole genome shotgun sequence.
ACCESSION   DATRQA010000264 DATRQA010000000
VERSION     DATRQA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.265"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..683)
                     /locus_tag="VBD27_002108"
     CDS             complement(<1..683)
                     /locus_tag="VBD27_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO7026067.1"
                     /translation="MATLTNNPIFGIMLTVWAYYIGIRIFRKYPSPATTPLLLATILL
                     IAFLKLTHISYKDYYNGGSFLTMLITPSTVVLAIPLYRTFHLMKHHIKSISISIILAS
                     VINTVFTAIVAKFFGMKYFLAISLFPKSVTTAMAVGITSKAGGLATITLVVVVITGIL
                     TSVLGPIFLKLLKIEDPVAIGLALGGTGHAIGTGQALKYGQVQGAMAGLAIGITGICY
                     VIVSPLVAG"
     gene            complement(664..>1002)
                     /locus_tag="VBD27_002109"
     CDS             complement(664..>1002)
                     /locus_tag="VBD27_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO7026068.1"
                     /translation="VLSTIFNLPIPGSIIGLILLFLALKYKIIRLRHIDTVGNFLLAN
                     MTILFLPPAVGLMEHFQDIKPYLFGIAIIILGALFLNILTIGLVSQWIKKRYEGDYPE
                     IGGKNGNFNE"
BASE COUNT          342 a          179 c          183 g          298 t
ORIGIN      
        1 aagcctgcaa ctaaaggact gacaataaca tagcaaattc ctgtgatacc aattgctagt
       61 ccagccatgg cgccttgaac ttggccatat tttaaggctt gtccagttcc tatagcatgc
      121 ccagttcctc ctagcgcgag ccctatagca acaggatctt ctatttttaa taattttaga
      181 aaaataggcc ccaaaacgct tgttaagatg cctgtaataa caactacgac cagagtgata
      241 gtagctaatc cacctgcttt acttgtaatt ccaacggcca tagctgtagt tacggacttt
      301 gggaaaagtg agattgctaa gaaatatttc ataccaaaaa acttagcaac tattgcggta
      361 aaaactgtgt tgataacaga agctaagatg atgctaatgc taatactttt aatgtgatgc
      421 ttcattaaat gaaaggttct atagagagga atagccaata caaccgttga aggcgtaatt
      481 aacatcgtta gaaatgaacc accattgtag taatctttat aggagatatg tgttaatttt
      541 aaaaaagcaa ttaataaaat agtcgccaaa agaagtggag ttgttgcagg ggaaggatat
      601 tttctaaaaa tccttatgcc aatgtaatac gcccatacgg ttaacataat accaaagata
      661 gggttattcg ttaaagttgc catttttacc tccaatttct ggataatctc cttcatatct
      721 ctttttgatc cattgggata ccagtccaat agttagaata ttgagaaata gcgctcctaa
      781 gataatgata gctatcccaa aaagataggg ctttatgtct tgaaagtgct ccatcagtcc
      841 aactgctggt ggtaaaaata aaatggtcat atttgctaat agaaaattcc ccacagtatc
      901 aatatgcctg agcctgatga ttttgtattt taacgctagg aatagtaata ttaatccaat
      961 aatactacct ggtataggta agttgaatat agtagataga ac
//
LOCUS       DATRQA010000265          997 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.266, whole genome shotgun sequence.
ACCESSION   DATRQA010000265 DATRQA010000000
VERSION     DATRQA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.266"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..461
                     /locus_tag="VBD27_002110"
     CDS             <1..461
                     /locus_tag="VBD27_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7026069.1"
                     /translation="EDPYYLAIVDNNTGKALGTFSLMRIDTKNRVVEMGWVVYSSKLK
                     QTRIATEAQYLVMKYVFEELCYRRYEWKCDSLNAPSNNSAKRLGFTFEGTFRQAVVYK
                     GRNRDTNWYSILDKEWPEKKTRFERWLDDSNFDVNEYQIRSLSSIEQKTK"
     gene            complement(721..>997)
                     /locus_tag="VBD27_002111"
     CDS             complement(721..>997)
                     /locus_tag="VBD27_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti sigma factor C-terminal domain-containing
                     protein"
                     /protein_id="HEO7026070.1"
                     /translation="LNTPQIFIDKIKENLKGNSKVYFNNINIYSDLEKYAKKYDKVNN
                     SDKLKFSGIILTGKAENFKQLKEKEWIHASSIGANIEYKPYYKLDKE"
BASE COUNT          327 a          131 c          164 g          375 t
ORIGIN      
        1 cagaagatcc ttattattta gctattgttg ataataatac tggtaaagca ttagggactt
       61 tttcattaat gcgtattgat acaaaaaata gagttgttga aatgggatgg gtggtttatt
      121 catcaaaact aaaacaaaca aggatagcaa cagaagctca atatttggtt atgaaatatg
      181 tttttgaaga actctgttac cgccgttatg agtggaaatg tgatagtttg aatgcaccat
      241 ctaataattc tgcaaaacga cttggtttta cttttgaagg aacttttaga caagctgttg
      301 tatataaggg tcgtaacaga gatacaaatt ggtattctat tttagataaa gaatggccag
      361 agaaaaaaac acggtttgaa agatggctag atgattcaaa ctttgatgta aatgagtatc
      421 aaatcagatc actatcaagc attgagcaga agacaaaata gagtcattca ttaatcacta
      481 aaatttcaaa attacatgtt tttttagcta acatcttaaa ctttgttaaa taaatgttta
      541 ttaactttta aaggtgcatg gtaggactca aaatcgggtg tcctcacgga cgtgctagct
      601 cgactttggt cgcgggcatc aaagaaaaaa gtcaagatta ttatcttgtc tttttttcta
      661 tgagttatta gctgtaaaag ttacgtaaaa ttaccaaaca aattttttaa caattaatta
      721 tcattcttta tctaatttat aatagggctt atattctatg tttgccccta tacttgaagc
      781 atgtatccat tctttttctt taagttgttt aaagttttcg gctttacctg ttaatattat
      841 tcccgaaaat tttaatttgt cgctattatt taccttgtcg tattttttgg catacttttc
      901 taaatctgag tagatattta tattattaaa gtatactttt gagttacctt ttaagttttc
      961 ttttatttta tctatgaata tttgtggtgt attcaaa
//
LOCUS       DATRQA010000266          996 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.267, whole genome shotgun sequence.
ACCESSION   DATRQA010000266 DATRQA010000000
VERSION     DATRQA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.267"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            235..987
                     /locus_tag="VBD27_002112"
     CDS             235..987
                     /locus_tag="VBD27_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7026071.1"
                     /translation="MFNKIGFRTWKSGKLWLYMGVLGSTIILGSSPVSAMDSVGNQSQ
                     GNVLERRQRDAENKSQGNVLERRQRDAENKSQGNVLERRQRDVENKSQGNVLERRQRD
                     AENKSQGNVLERRQRDVENKSQGNVLERRQRDAENKSQGNVLERRQRDADNKSQVGQL
                     IGKNPLFSKPTVSRENNHSSQGDSNKQSFSKKVSQVTNVANRPMLTNNSRTISVINKL
                     PKTGGDQNVIFKLVGFGLILLTSRCGLRRNEN"
BASE COUNT          375 a          138 c          195 g          288 t
ORIGIN      
        1 aaatcctaat gttagtatga ctatctatca tgttttataa tattaaagga aaatttaaaa
       61 atatcatgtt ttagatatca actatttaat tttaagcata caaattaata ataaattgta
      121 attaaataac aaattaactt ggcatagatt ataaaatgtt ttaatatata taatctaaat
      181 aaaaataata ataaaatgac ttttaaaatt taaaaaaagt aaggagaaaa ttaattgttc
      241 aataaaatag gttttagaac ttggaaatca ggaaagcttt ggctttatat gggagtgcta
      301 ggatcaacta ttattttagg atcaagtcct gtatctgcta tggatagtgt tggaaatcaa
      361 agtcaaggta atgttttaga gcgtcgccaa cgtgatgcgg aaaacaaaag tcagggtaat
      421 gttttagagc gtcgccaacg tgatgcggaa aacaagagcc aaggcaatgt tttagagcgt
      481 cgtcaacgcg atgttgagaa caagagccaa ggtaatgttc tagagcgtcg ccaacgtgat
      541 gcggaaaaca agagccaagg caatgtttta gagcgtcgtc aacgcgatgt tgagaataag
      601 agccaaggca atgttttaga gcgtcgtcaa cgtgatgcgg aaaacaaaag tcagggcaat
      661 gttctagagc gccgccaacg tgatgcggat aacaagagcc aagtaggtca acttataggg
      721 aaaaatccac ttttttcaaa gccaactgta tctagagaaa ataatcactc tagtcaaggt
      781 gactctaaca aacagtcatt ctctaaaaaa gtatctcagg ttactaatgt agctaataga
      841 ccgatgttaa ctaataattc tagaacaatt tcagtgataa ataaattacc taaaacaggt
      901 ggtgatcaaa atgtcatttt taaacttgta ggttttggtt taattttgtt aacaagtcgc
      961 tgcggtttga gacgcaatga aaattaagta taatca
//
LOCUS       DATRQA010000267          990 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.268, whole genome shotgun sequence.
ACCESSION   DATRQA010000267 DATRQA010000000
VERSION     DATRQA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.268"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            164..499
                     /locus_tag="VBD27_002113"
     CDS             164..499
                     /locus_tag="VBD27_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000827864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7026072.1"
                     /translation="MKRNKHLPLTETTYYILLALFEEAHGYAIMKKVEEMSGGDVRIA
                     AGTMYGAIENLLKQKWIKSISSDDRRRKVYIITETGKEIVELETNRLRKLLNTANQLG
                     FGGDGYDKV"
     gene            486..>990
                     /locus_tag="VBD27_002114"
     CDS             486..>990
                     /locus_tag="VBD27_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEO7026073.1"
                     /translation="MIKFKLFFKSTEKEEEWIDKVQKSGYTFTGINSFLPAFYYFKRD
                     KEKDKMSIKMDFKKYMSKDDYTNYITLFEDSGWKHISGSRWGGLHYFQKIKPETSSEI
                     FSDQESKSAMAMSYYKYTCEHAFFSLFYFFLLIRTTSITIFDPKSWYLTQGLWEMQGK
                     IFWLGFLF"
BASE COUNT          369 a          117 c          187 g          317 t
ORIGIN      
        1 atagtacttt tgagaagtta cgactaaaat aaaaaactcc aaacgaataa ttggataggc
       61 tatctgttct tttcttttta ataagtttaa atatacagtt gacctatata gttatattat
      121 atagaataac tatataaagt ttaactgtat agaaagaaaa ttgatgaaaa ggaataaaca
      181 tttaccgtta acagaaacta cctattatat tttattagct ttgtttgagg aagcgcatgg
      241 ctatgctatt atgaaaaaag ttgaagaaat gagtggcggt gatgttagaa tagccgcagg
      301 gacaatgtac ggtgccattg aaaatttact taaacaaaaa tggataaagt ctatctcaag
      361 tgacgataga agaagaaaag tttatattat tactgagaca ggaaaagaaa tagtagaact
      421 tgaaacgaat cgattaagaa agttacttaa tactgctaat cagttgggtt ttggaggaga
      481 tggttatgat aaagtttaaa ttatttttta aatccacaga aaaagaggaa gaatggatcg
      541 ataaggttca aaaaagtggc tatactttta caggcattaa ctcatttctt ccagccttct
      601 attattttaa gagagataaa gaaaaagaca agatgtcaat aaaaatggat tttaaaaaat
      661 acatgtcgaa agatgactat actaactata tcactttatt tgaggacagt ggatggaagc
      721 atattagcgg tagtcgttgg ggagggctgc attattttca aaaaataaag cctgaaacca
      781 gttcagagat tttttctgat caagaatcaa aatcagcgat ggctatgagt tattataaat
      841 atacttgtga gcatgcgttt ttctctttat tttatttctt tctcttgata agaacaactt
      901 ctatcaccat atttgatcca aaaagttggt atctgacgca aggtctatgg gaaatgcaag
      961 ggaaaatatt ttggttaggg ttcttatttg
//
LOCUS       DATRQA010000268          977 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.269, whole genome shotgun sequence.
ACCESSION   DATRQA010000268 DATRQA010000000
VERSION     DATRQA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.269"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..963)
                     /locus_tag="VBD27_002115"
     CDS             complement(<1..963)
                     /locus_tag="VBD27_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO7026074.1"
                     /translation="MEKYTSIKQSGRHYGLDLLRIISMFMIVITHVLGKGGLRSSVEG
                     HADSYFIVTWIIQVLVYGAVNCYALISGYVGINSRYRYSKLLSIWTQVFFYTFTITAL
                     FAITGHEVTLLNWRDAFFPIVSGQYWYITAYFGLLVFMPVINNGLNALTDKQLKQLVL
                     LMFIIFSILPAVLNNRVPEFSLSKGFEMTWLLILYIIGAYLKRIDLNIFKTSYLLIVY
                     LLSLVATYVMKFSIGDIWYWYVSPTLTLGAVSLFILFARASIKPSGFLKKIIVVLAPS
                     TLGVYLCHLHPLIVKYFVRDFAETFVYESIYLYPFLILGAGILIY"
BASE COUNT          387 a          180 c          143 g          267 t
ORIGIN      
        1 ataaattaaa atccctgctc ctaaaattaa aaatgggtaa agataaatgg actcataaac
       61 aaacgtctct gcaaaatctc ttacaaaata ttttactatt agtgggtgga gatgacagag
      121 ataaactccc aaggttgaag gggctaatac aacaattatt ttctttaaga atcccgaggg
      181 cttaatgctt gctctagcaa ataatataaa caaacttacc gcccctaaag ttaaagtagg
      241 cgaaacatac caataccaaa tatcaccaat actaaacttc atgacataag tagcaaccaa
      301 tgataataga taaactataa gtaagtatga tgttttaaat atatttaaat caatacgttt
      361 caaataggca ccaataatat ataaaatcag tagccaagtc atttcaaaac ccttgctcaa
      421 tgaaaattct ggaacacgat tgtttaaaac agctggtaaa attgaaaaaa taataaacat
      481 taacaaaacc aattgtttta attgtttatc agtcaaggca tttaatccat tattaataac
      541 aggcataaaa accaatagac caaagtaagc agtaatgtac caatactgcc cactgacaat
      601 agggaaaaaa gcatctctcc aattaagcaa ggtcacttca tgacctgtga ttgcaaaaag
      661 tgctgttatt gtaaaggtgt agaaaaaaac ctgtgtccag atagacagta attttgagta
      721 acgataacgc gaattaattc ccacataacc acttattagt gcgtaacagt taacggcccc
      781 ataaactaag acttggataa tccacgttac tataaaatag gagtctgcgt gtccctcaac
      841 cgaagatctc agaccacctt ttccaagaac atgcgtaata acaatcatga acatggaaat
      901 aatccttaaa aggtctaagc cgtaatgacg acctgattgt ttaatacttg tatacttttc
      961 cataatcata ttttagc
//
LOCUS       DATRQA010000269          977 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.270, whole genome shotgun sequence.
ACCESSION   DATRQA010000269 DATRQA010000000
VERSION     DATRQA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.270"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..>977
                     /locus_tag="VBD27_002116"
     CDS             <1..>977
                     /locus_tag="VBD27_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7026075.1"
                     /translation="INTKGIIFASTNPKRVGEFHEIGLKVAQTGQMIEVTDQESYFGT
                     QAGINIPFYYNCELLATIGISGNPNQVGKYALLAQKMTRLILKEHELDYLDFGRKNEA
                     SIVLHHLVEGRELDYYYLNQFLNQYHLSEKTDYRLLTFEINSQKQKLLLSQSEMSLLN
                     FFDKLDTAIYTFNYPNQYWLLLSDQMFDYYYPNILSKFECEKGLYKVGIGQKSSLSLL
                     KRSYETSILALKALKGQQKVNLVDDLDLELLLTSIDSNIKQYVLNKALVNLSENDKIL
                     LNSYFKHNLSLKECSQELFIHKNTVQYRLNKIYESTQLNPRNFKDATLL"
BASE COUNT          357 a          169 c          140 g          311 t
ORIGIN      
        1 ttattaatac caaaggaatc attttcgcta gtaccaatcc aaaacgtgtt ggagaattcc
       61 atgaaattgg tttaaaagta gcacagacag gacaaatgat tgaagtaaca gatcaagaat
      121 cttattttgg gacacaagca gggatcaata ttcctttcta ttataattgt gaattattag
      181 ctactattgg gattagtgga aaccccaacc aagtaggaaa atatgcttta cttgctcaaa
      241 aaatgactcg actcatctta aaagagcatg aattagatta tctggatttc gggcgaaaaa
      301 acgaagcttc tattgtctta caccatcttg ttgaaggcag agagttagac tactattacc
      361 tcaatcaatt tttaaaccaa taccacctat cagaaaaaac tgactatcgt ctactcactt
      421 ttgaaatcaa tagtcaaaaa caaaaattac tcttaagtca atctgaaatg tcactcttga
      481 acttttttga taaacttgac actgctattt atacctttaa ttatcccaat caatactggc
      541 ttttactttc tgaccagatg tttgactatt attatccaaa tatcctatca aagtttgaat
      601 gcgaaaaggg tctctataaa gttggtatcg gacaaaaatc atctttatca cttttaaaaa
      661 gatcatatga aacgtctatc ttagccctga aagctttaaa aggtcaacaa aaggtcaacc
      721 tcgtcgacga ccttgatctt gaactgttat taacaagtat tgattctaat attaaacaat
      781 atgtgctaaa taaagcgctt gtaaacttat ctgaaaatga taagatcttg cttaactcat
      841 attttaagca taatctatca ttaaaagaat gtagtcagga actttttatc cataaaaata
      901 ctgttcaata taggttaaat aagatttatg aatcaacgca actcaatcca agaaacttta
      961 aggatgcaac tttattg
//
LOCUS       DATRQA010000270          972 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.271, whole genome shotgun sequence.
ACCESSION   DATRQA010000270 DATRQA010000000
VERSION     DATRQA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.271"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(34..900)
                     /locus_tag="VBD27_002117"
     CDS             complement(34..900)
                     /locus_tag="VBD27_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEO7026076.1"
                     /translation="MEGLLIALIPMFAWGSIGFVSNKIGGRPNQQTFGMTLGALLFAI
                     IVWLFKQPEMTASLWIFGILGGILWSVGQNGQFQAMKYMGVSVANPLSSGAQLVGGSL
                     VGALVFHEWTKPIQFILGLTALTLLVIGFYFSSKRDVSEQALATHQEFSKGFATIAYS
                     TVGYISYAVLFNNIMKFDAMAVILPMAVGMCLGAICFMKFRVNFEAVVVKNMITGLMW
                     GVGNVFMLLAAAKAGLAIAFSFSQLGVIISIIGGILFLGETKTKKEQKWVVMGILCFV
                     MGAILLGIVKSY"
BASE COUNT          351 a          215 c          136 g          270 t
ORIGIN      
        1 tgaaaaaaaa gcaacagatt aatctgttgc tttttaataa gatttaacaa taccaagtaa
       61 tatagcaccc ataacaaaac aaaggatacc catgacaacc catttctgct ctttcttcgt
      121 ttttgtctca cctaaaaata aaataccacc aataatagag ataattactc caagttgaga
      181 aaaactaaaa gcaattgcta gccctgcttt agctgctgcc aataacatga agacattacc
      241 aacgccccac atgagacctg taatcatatt tttaacaaca acagcctcaa agttaacacg
      301 aaacttcatg aaacaaattg cacctagaca cattccaaca gccatgggta aaatgacggc
      361 catagcgtcg aacttcataa tgttgttaaa taaaactgcg tacgagatgt aacctacagt
      421 tgaataagca attgtagcaa atccttttga aaactcttga tgtgttgcca aagcttgttc
      481 tgaaacatca cgtttacttg agaaatagaa gccgataact aataatgtca acgctgtcaa
      541 tcctaaaata aattggattg gcttagtcca ttcatgaaag actaaagcac caactaggct
      601 tccacctact aattgtgcac cacttgacag tggattagca acagagactc ccatatattt
      661 cattgcttga aattgaccat tttggccgac tgaccatagg ataccaccta agataccaaa
      721 aatccacaat gaggcagtca tctctggctg tttaaataac catacgataa tcgcaaatag
      781 caatgctcct aaagtcattc caaatgtttg ttgatttgga cgccctccaa ttttattact
      841 aacaaatcca atacttcccc acgcaaacat gggaatcaat gcaataagta aaccttccaa
      901 ttctttctcc ttacaatgga atattaagta ttacctattt tcaatcctag agaatctatt
      961 gactaacttt at
//
LOCUS       DATRQA010000271          969 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.273, whole genome shotgun sequence.
ACCESSION   DATRQA010000271 DATRQA010000000
VERSION     DATRQA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.273"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            88..327
                     /locus_tag="VBD27_002118"
     CDS             88..327
                     /locus_tag="VBD27_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7026077.1"
                     /translation="MTLFLNSLLKELIIVNRLREYRIKASISQLALAKSVGVARQTIN
                     LIENNKYNPSLDLCIRLAECLQTDLNTLFWDSRHL"
     gene            427..960
                     /locus_tag="VBD27_002119"
     CDS             427..960
                     /locus_tag="VBD27_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEO7026078.1"
                     /translation="MKEKKNLGIKLIKSLYGINGPFDEYREQKINYLGNLLFATTSLY
                     HILSSIIAWFTVSKYPTETACTLLALTFFLYFCIFLIVIPSYLNSHDLITPLEVSKND
                     YPVAIKKNKQKFIKIGIFYTVIQWLILGLTTNNNTPLIVSLFSLKVILSSIMSGVAYN
                     GVMYLIFKNNVDIEKDE"
BASE COUNT          351 a          160 c          133 g          325 t
ORIGIN      
        1 tgactggaaa caagtttttt atatttattt gtcacatata attgacactc ttcgaaaaat
       61 gatatatact taaaatgtca tctatatatg acacttttct taaattcttt actaaaggag
      121 ctaataatcg tgaatcgtct tagagaatac agaattaaag ctagcatatc tcaattagct
      181 ttagcaaaat cagttggtgt cgccagacaa acaataaacc ttattgaaaa taataaatac
      241 aatccttcgc tagatctttg tataagacta gcggaatgcc tacaaacaga cctcaatact
      301 cttttttggg atagtagaca tctataacag attagactct ctaaatctat gaaaactcgg
      361 gaggttaggt cgtattatta tttcaatctc aaactttatt aaatagttca aggataggag
      421 aatattatga aagaaaagaa aaatttgggg ataaaattaa taaaatccct atatggtatt
      481 aatggcccat tcgatgaata cagagaacaa aaaatcaatt accttggaaa tctattattt
      541 gcaaccacta gtctctatca cattttatcc tctatcatcg cctggttcac ggtaagtaaa
      601 tatcccacag agactgcatg tactctccta gctttaacat tctttctata tttttgtatt
      661 tttctaattg ttattccctc ttatctcaac agtcatgatt taataacgcc actagaagtc
      721 tcaaaaaatg actatccagt agcaataaag aaaaacaaac aaaaatttat caaaatcggg
      781 atattttata ctgtcatcca gtggcttatc ttgggtttaa caactaacaa taacactccg
      841 ctgattgtat cactattttc tttaaaagtt attttgtcta gtattatgtc cggtgtagct
      901 tacaatggag ttatgtatct aatatttaaa aacaatgttg atattgaaaa agatgaataa
      961 tgacaacat
//
LOCUS       DATRQA010000272          965 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.274, whole genome shotgun sequence.
ACCESSION   DATRQA010000272 DATRQA010000000
VERSION     DATRQA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.274"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..735
                     /locus_tag="VBD27_002120"
     CDS             <1..735
                     /locus_tag="VBD27_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7026079.1"
                     /translation="SISTVSRSDVNIIMTVNTNTHKVLLTTTPRDAYVKIPDGGGNQY
                     DKLTHAGLYGVETSMKTLENLYDINLDYYARINFSSFLKLIDLLGGVTVYNDQAFTSK
                     HGNFDFPVGQVTLNSEQALGFVRERYSLQGGDNDRGRNQEKVIAAIINKLASSQSVTK
                     LNSITSQLQTSVQTNMTIDNINDLINNQLSTGQRFTVESQALTGHGSTGELPSYAMPG
                     AQLYMMSIDQSSLSNAKSKIKNTMEE"
     gene            741..>965
                     /locus_tag="VBD27_002121"
     CDS             741..>965
                     /locus_tag="VBD27_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEO7026080.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQI"
BASE COUNT          349 a          156 c          169 g          291 t
ORIGIN      
        1 tctatatcaa cagtatcaag atctgatgta aatattatta tgacggttaa taccaatacc
       61 cacaaagtat tgttaacgac aacaccacga gatgcctatg taaaaattcc agatggtggg
      121 ggcaatcaat atgataaatt aacccatgca ggtttgtatg gtgttgagac atcaatgaaa
      181 acacttgaaa acctttacga catcaacctt gattattatg ctagaattaa tttttcatca
      241 tttttaaaat taatagacct cttgggagga gtgacagttt ataacgatca agcttttaca
      301 agtaaacatg gtaattttga ctttcctgtt ggtcaagtaa cattgaattc tgagcaggct
      361 ttgggctttg ttagagaacg ttattctcta caaggaggcg ataacgatag aggtagaaat
      421 caagaaaaag tgattgcagc tattataaat aagttagctt ctagtcagtc agtaacaaaa
      481 ttaaatagca ttacctcaca gctccaaacg tccgttcaaa ctaatatgac tattgataat
      541 attaatgatt tgattaacaa tcaattgtca actggacaac gcttcactgt cgagtcacaa
      601 gcattaactg gtcatggttc aacgggtgaa ctcccttcat atgcaatgcc aggagctcaa
      661 ctttatatga tgtcaattga tcaatctagc ttatctaatg caaaatcaaa aattaagaac
      721 acaatggagg aataacaaga atgattgata ttcattctca tatcgttttt gatgtagatg
      781 atgggcccaa aacgcttgaa gagagtttat ctttaataga agaaagttat cgacaaggtg
      841 ttagaataat tgtttcgaca tcacatagaa gaaaaggtat gtttgaaaca cctgaagata
      901 ttatcttcaa aaacttttct atagttaaac acgaagcgga aaaaagattt gaacatcttc
      961 aaatc
//
LOCUS       DATRQA010000273          962 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.275, whole genome shotgun sequence.
ACCESSION   DATRQA010000273 DATRQA010000000
VERSION     DATRQA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.275"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..173
                     /locus_tag="VBD27_002122"
     CDS             <1..173
                     /locus_tag="VBD27_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEO7026081.1"
                     /translation="RCPHPKLINVLERKLEIILGDQKHILEKDSLISLSPQETHHLRA
                     IENSKFLQIELD"
     gene            244..314
                     /locus_tag="VBD27_002123"
     tRNA            244..314
                     /locus_tag="VBD27_002123"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:276..278,aa:Thr,seq:ggt)
     gene            complement(450..920)
                     /locus_tag="VBD27_002124"
     CDS             complement(450..920)
                     /locus_tag="VBD27_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001889031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO7026082.1"
                     /translation="MVYFRKQFYRVTWDDIDFERNSIYTYRRYSSIKNDFTKPKTRTS
                     IRRVSVSHDLKELLLELKFQQERMLKGLHIKNTDQLIFYDYRFGMISNDAVNKFLASS
                     LEKLEIESKMSSTGARHTYGSYLLANGVDIWAVAKLMGHKDIKQLIETYGHLLL"
BASE COUNT          334 a          159 c          118 g          351 t
ORIGIN      
        1 aacgatgccc tcatccaaaa ttaataaatg ttttggaaag aaaacttgaa attatccttg
       61 gtgatcagaa acatatcctt gaaaaagata gccttattag cctctctcca caagaaacac
      121 accatttaag agctattgaa aatagtaaat ttctccaaat agaattagac taaaaaaacg
      181 actatttttc taagtaaaga aatacttgat ttcgtcaaaa tcttatagta tcatagaaaa
      241 gtagccgcta tagttaaatg gtataataga gcaatggtaa tgctcaattc cgagttcaat
      301 tctcggtggt ggcagaaaat atatgagtga aaccttatta taatagggtt tcttttattt
      361 ttgttcttaa tttgttcttg atagtcattt taaaaactac ctttattatt aagaattttt
      421 aaaagctgat cattttcctt ttcttccact tacagaagca aatgaccata tgtttctatt
      481 aattgtttga tatctttatg ccccattaat tttgcaaccg cccaaatatc tactccgtta
      541 gccaacaaat aacttccata agtatgtcta gcacctgttg atgacatctt agattctatt
      601 tctaattttt ccaatgagct tgccaagaat ttgttaacag catcatttga tatcatacca
      661 aacctatagt cataaaaaat caattgatct gtatttttta tatgcaaacc tttcagcatt
      721 ctttcttgtt gaaattttag ttcaagcaat aactctttta aatcatgact tactgaaact
      781 ctccttattg aagtccttgt tttaggcttt gtaaagtcat tttttattga agaatatctt
      841 cgatatgtat aaatactatt tctttcaaaa tcaatatcat cccaagttac tctataaaat
      901 tgtttacgaa aataaactat tcaaagaaat aaccattgat ttagaaattt caaaggaatg
      961 tt
//
LOCUS       DATRQA010000274          960 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.276, whole genome shotgun sequence.
ACCESSION   DATRQA010000274 DATRQA010000000
VERSION     DATRQA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.276"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..234)
                     /locus_tag="VBD27_002125"
     CDS             complement(<1..234)
                     /locus_tag="VBD27_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7026083.1"
                     /translation="MFVMRTFGNSLSGPLVVILSSILFSWSHLHGLSVVDFVVYFGMG
                     LIFASLHHYTKSIHYSIGEHIVWNSLSYIFYFLA"
     gene            complement(253..504)
                     /locus_tag="VBD27_002126"
     CDS             complement(253..504)
                     /locus_tag="VBD27_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026084.1"
                     /translation="MMILLSYVLTKTEPISNYQESSSNLKKWLFAIGAFSVLFCWALL
                     VKTFFPIRQNQEGLIADMNQVNHYFRVVLLLTICLIAPI"
BASE COUNT          356 a          171 c          155 g          278 t
ORIGIN      
        1 tgctagaaaa taaaaaatat atgacaaact attccaaaca atatgctcgc caatcgaata
       61 atgaatggat tttgtatagt ggtgcaaact agcgaaaatc aaacccatac caaaatagac
      121 aacaaagtcg acgacagata gaccatgtaa gtgagaccag ctaaatagta tagaagataa
      181 gatcacaacc aagggaccac tcagggagtt cccaaatgtt ctcatgacaa acaacctgaa
      241 cgttatctct tcttaaattg gggcaataag acagatagtc aataataaga caactcgaaa
      301 gtagtgattg acctgattca tatcagctat taatccctcc tggttttgcc ttattgggaa
      361 aaatgttttt actagtagtg cccagcaaaa taaaacgcta aaagcaccta ttgcaaatag
      421 ccattttttg aggttagatg atgactcttg gtagttactg attggctcag tttttgttag
      481 cacataactc aacagaatca tcattataag attaatgcca atccatatac tccaactcca
      541 ctctaaccct attgatagta aactaatcac ccaatgccca agcaattgtt attattgtgt
      601 tgttgcaact ttggcaaaga tgaatatagc aggatggaaa acagattgtt tctgaaactt
      661 caaaggtatc ttcttatcac aagctattat ttggttatct ccataattaa tctaaatgga
      721 gcaatatatc gtttttctac taactgttga caaataaaaa actccccttt aaagtctggt
      781 aacttttcga tttttaccag aaacttaagg agagtgaggc aagaactaat agaatcttat
      841 ttaaagtttt atgattggga gaacagaaca actatcataa ttactgaaaa attatgaaag
      901 gcatggcaaa caacagatgg tcaaatccgc tctgttgaaa acaaatttta aatataattc
//
LOCUS       DATRQA010000275          944 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.277, whole genome shotgun sequence.
ACCESSION   DATRQA010000275 DATRQA010000000
VERSION     DATRQA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.277"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            162..929
                     /gene="cfb"
                     /locus_tag="VBD27_002127"
     CDS             162..929
                     /gene="cfb"
                     /locus_tag="VBD27_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb"
                     /protein_id="HEO7026085.1"
                     /translation="MNVKHMMYLSGTLVAGALLFSPAVLEVHADQVTTPQVVNHVNSN
                     NQAQQMAQKLDQDSIQLRNIKDNVQGTDYEKPVNEAITSVEKLKTSLRANPETVYDLN
                     SIGSRVEALTDVIEAITFSTQHLTNKVSQANIDMGFGITKLVIRILDPFASVDSIKAQ
                     VNDVKALEQKVLTYPDLKPTDRATIYTKSKLDKEIWNTRFTRDKKVLNVKEFKVYNTL
                     NKAITHAVGVQLNPNVTVQQVDQEIVTLQAALQTALK"
BASE COUNT          357 a          136 c          169 g          282 t
ORIGIN      
        1 agttttatga aaaaaatgca gtttatttgt aataatgtaa aatatagggg aaaagaaagc
       61 gcttgacgac cttttggaca agtagtaaga tacaaacatg ggccctgtaa attaaaaata
      121 ctgcagtaaa agtgatttta gtttaaagga ggaaatttat tatgaacgtt aaacatatga
      181 tgtatctatc tggaactcta gtggctggtg cattgttatt ttcaccagct gtattagaag
      241 tacatgctga tcaagtgaca actccacaag tggtaaatca tgtaaatagt aataatcaag
      301 cccagcaaat ggctcaaaag cttgatcaag atagcattca gttgagaaat atcaaagata
      361 atgttcaggg aacagattat gaaaaaccgg ttaatgaggc tattactagc gttgaaaaat
      421 taaagacttc attgcgtgcc aaccctgaga cagtttatga tttgaattct attggtagtc
      481 gtgtagaagc cttaacagat gtgattgaag caatcacttt ttcaactcaa catttaacaa
      541 ataaggttag tcaagcaaat attgatatgg gatttgggat aactaagcta gttattcgca
      601 ttttagatcc atttgcttca gttgattcaa ttaaagctca agttaacgat gtaaaggcat
      661 tagaacaaaa agttttaact tatcctgatt taaaaccaac tgatagagct accatctata
      721 caaaatcaaa acttgataag gaaatctgga atacacgctt tactagagat aaaaaagtac
      781 ttaacgtcaa agaatttaaa gtttacaata ctttaaataa agcaatcaca catgctgttg
      841 gagttcagtt gaatccaaat gttacggtac aacaagttga tcaagagatt gtaacattac
      901 aagcagcact tcaaacagca ttaaaataat atttgtattt ttcg
//
LOCUS       DATRQA010000276          944 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.278, whole genome shotgun sequence.
ACCESSION   DATRQA010000276 DATRQA010000000
VERSION     DATRQA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.278"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            90..>944
                     /gene="ltrA"
                     /locus_tag="VBD27_002128"
     CDS             90..>944
                     /gene="ltrA"
                     /locus_tag="VBD27_002128"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO7026086.1"
                     /translation="MSELLDKISSRNNMLEAYKQVKSNKGSAGIDGVTIEQMDDYLHQ
                     NWRETKKLIKERSYKPQPVLRVEIPKPNGGVRNLGIPTAMDRMIQQAIVQVLSPLCEK
                     HFSEYSYGFRPNRSCETAIVQLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     QDGDTESLIRKYFHSGVVINGQRHKTLVGTPQGGNLSPLLSNIMLNELDKGLEKRGLR
                     FVRYADDCVITVGSEAAAKRVMHSVSSYIEKRLGLKVNMTKTKIVRPNKLKYLGFGFW
                     KSPKGWKCR"
BASE COUNT          295 a          189 c          223 g          237 t
ORIGIN      
        1 tagaactaga tgcggttacg tattgactag aggaaggttc accaatgtaa gatatcatgc
       61 agacaacgaa aaaagaaagg aatacgcaca tgtcagaatt gctagataag atatcatctc
      121 ggaacaatat gctcgaagct tacaagcaag tgaaatcaaa caaaggttct gctggtatcg
      181 atggggtcac tatcgagcag atggatgact atcttcacca aaattggcga gaaaccaaga
      241 aactcatcaa agagaggagc tataaacctc aaccggttct cagggttgaa atcccaaaac
      301 caaacggagg agttcgtaac ctaggtatcc cgacggctat ggatagaatg attcagcagg
      361 ccatcgttca agttttgagt ccactctgcg aaaaacattt ttcagagtat agctatgggt
      421 tcagacccaa tcgctcctgc gaaacagcca ttgttcagct acttgagtat ttaaacgatg
      481 gctacgagtg gattgtggac attgacttgg aaaagttctt cgatactgtt ccgcaagaca
      541 gattgatgtc cctggttcat aatatcattc aagatggcga tacggagtca ctgattcgta
      601 agtacttcca ttcgggagtt gttattaacg gacagcgaca taagacttta gtcgggacac
      661 ctcaaggcgg gaatctatca cccctcctat ctaatattat gcttaatgag ttagacaaag
      721 ggttggaaaa gcgaggtctt cgctttgtcc gttacgccga tgactgtgtc atcactgtcg
      781 gaagcgaagc agctgctaag cgggtcatgc attcggtcag tagctatatt gagaagcgat
      841 tagggttgaa agtcaacatg actaagacca agattgtcag accgaacaaa ctcaaatacc
      901 tcggatttgg tttctggaaa tctccaaaag gttggaagtg tcgt
//
LOCUS       DATRQA010000277          936 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.279, whole genome shotgun sequence.
ACCESSION   DATRQA010000277 DATRQA010000000
VERSION     DATRQA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.279"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            1..520
                     /locus_tag="VBD27_002129"
                     /pseudo
     CDS             1..520
                     /locus_tag="VBD27_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584204.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            628..>936
                     /locus_tag="VBD27_002130"
     CDS             628..>936
                     /locus_tag="VBD27_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026087.1"
                     /translation="MEVKKFSEKDFVNEINKIKQKQFLSQIEQYESYIAPQMRTKGYK
                     RINQSERTVVFSFGEITFSRSRWTNGFETRIPVDEWLGLEKYKRYSIEFLYHVAKLAT
                     M"
BASE COUNT          369 a          111 c          174 g          282 t
ORIGIN      
        1 ttatttacag gttctgaaag cagtaatact tctaaaaaga ataagacaaa tcctagtggt
       61 gcgacaagtg caaaagtcgt taaacacaaa aaaactgatg aagaacgagc agaagattat
      121 ataaaaacgt taggattaaa cccgagtgat tatataaaag aaatggatta ttcaaaggtt
      181 gattttaggg ggggatttat catcagtatc aggtaattat aataatttta ctgttgaaaa
      241 catcaatcaa acttttgaaa aaggtgataa atctttaaaa gatattattg aaatttttgg
      301 aaagcctgat acaatttggg ttaattacgt tgacaatcag tacaatactg atttgtactg
      361 gaaaaaagat gacacagatg attctaatta tattcatgca gaatggtctg agccaaaaga
      421 tgaatatgat gttcaaagtt taagtggcac ggcttcaaat tcaggtactg gcaagtttca
      481 gtcattcatt ggattagatg gatttgagcc tgcacagtag gataaataga aagatcacgt
      541 tattaaatgt ggtcttttac tttaactttt cgaataaaaa atagcaataa tattctaatc
      601 ctgtaacaaa aattgtaaaa tattatcatg gaagttaaaa aattctcgga aaaagatttt
      661 gtaaatgaaa taaataaaat aaaacagaaa caatttttaa gtcaaattga acagtatgaa
      721 agctatatcg ctcctcaaat gagaacgaaa ggctataaga ggatcaatca gtctgaacgt
      781 acggttgttt tctcttttgg agaaataaca tttagtagga gtcgctggac aaatggcttt
      841 gaaactagaa taccagtaga tgagtggtta ggtcttgaaa aatataagag atattcaata
      901 gaattcttat atcatgttgc aaaattggct acaatg
//
LOCUS       DATRQA010000278          865 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.280, whole genome shotgun sequence.
ACCESSION   DATRQA010000278 DATRQA010000000
VERSION     DATRQA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.280"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(1..73)
                     /locus_tag="VBD27_002131"
     tRNA            complement(1..73)
                     /locus_tag="VBD27_002131"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Ile,seq:cat)
     gene            complement(93..166)
                     /locus_tag="VBD27_002132"
     tRNA            complement(93..166)
                     /locus_tag="VBD27_002132"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Met,seq:cat)
     gene            complement(182..255)
                     /locus_tag="VBD27_002133"
     tRNA            complement(182..255)
                     /locus_tag="VBD27_002133"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="VBD27_002134"
     tRNA            complement(263..336)
                     /locus_tag="VBD27_002134"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(348..432)
                     /locus_tag="VBD27_002135"
     tRNA            complement(348..432)
                     /locus_tag="VBD27_002135"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(396..398),aa:Leu,seq:taa)
     gene            complement(441..512)
                     /locus_tag="VBD27_002136"
     tRNA            complement(441..512)
                     /locus_tag="VBD27_002136"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Gly,seq:gcc)
     gene            complement(546..618)
                     /locus_tag="VBD27_002137"
     tRNA            complement(546..618)
                     /locus_tag="VBD27_002137"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(583..585),aa:Thr,seq:tgt)
     gene            complement(629..710)
                     /locus_tag="VBD27_002138"
     tRNA            complement(629..710)
                     /locus_tag="VBD27_002138"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(674..676),aa:Leu,seq:tag)
     gene            complement(720..792)
                     /locus_tag="VBD27_002139"
     tRNA            complement(720..792)
                     /locus_tag="VBD27_002139"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(757..759),aa:Lys,seq:ttt)
     gene            complement(795..865)
                     /locus_tag="VBD27_002140"
     tRNA            complement(795..865)
                     /locus_tag="VBD27_002140"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Asp,seq:gtc)
BASE COUNT          236 a          251 c          196 g          182 t
ORIGIN      
        1 ggaccttgta ggactcgaac ctacgaccgc tcggttatga gccgagtgct ctaaccagtt
       61 gagctaaagg tccaaagtct caataaaata aatagcggcg gaggggatcg aacccccgac
      121 ctcccgggta tgaaccggac gctctagcca gctgagctac accgccataa aaatatatcc
      181 atcgggaaga caggattcga acctgcgaca ccttggtccc aaaccaagta ctctaccaag
      241 ctgagctact tcccgaaaaa tatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctaaa tattatatgc cgaggaccgg
      361 aatcgaaccg gtacgatgtt taccatcgca ggattttaag tcctgtgcgt ctgccagttc
      421 cgccaccccg gcctctaaca agcgaacgac ggggttcgaa cccgcgaccc tcaccttggc
      481 aaggtgatgt tctaccactg aactacgttc gcactaaaga cactatttat cctataaaat
      541 tgtaatgccg gctacatgac ttgaacacgc gaccctctga ttacaaatca gatgctctac
      601 caactgagct aagccggcaa tctactaatg cgggtgaagg gacttgaacc cccacgccgt
      661 taagcgccag atcctaaatc tggtgcgtct gccaattccg ccacacccgc atttctaaat
      721 gacccgtact gggctcgaac cagtgaccca ttgattaaaa gtcaattgct ctaccaactg
      781 agctaacgag tctacggtcc cgacgggaat cgaacccgcg atcttcgccg tgacagggcg
      841 acgtgataac cgctacacta cggga
//
LOCUS       DATRQA010000279          836 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.281, whole genome shotgun sequence.
ACCESSION   DATRQA010000279 DATRQA010000000
VERSION     DATRQA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.281"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
BASE COUNT          346 a          125 c          106 g          259 t
ORIGIN      
        1 ccactatacc aaaagttgag aataataata caatactcca atgattagga tctatttcct
       61 caaagagcgt gtaacataat aacgtataaa aaaataatct gtagatttta taattttttg
      121 aaatcgcttc tttatataaa agtataaagg cagaaaaata aagaacaatt cctaataaag
      181 aagtcctgta aaagacacta atataagtag aatgcgatcc taggaatatt ccttcacttg
      241 atggaatcct tataccctgt ccaaataaaa tatttccttt tagaacttca ataatacttt
      301 cttgataaac tatcatccta gtgatagtac tttggtttct gtacataatt attttttcaa
      361 ttattgacca tatgttataa gaaaaacaag cggtgataaa taagaaagtc ataccaagta
      421 taaaagtgtt tttcttggtc aatttcaaat tagttttaaa aaggatataa tctaaaagaa
      481 gcgaagctaa taaaaccaac atacagacca atagtattct agcgccagaa aagtatgaac
      541 atatagtcaa cagtaaggat attaaaatat tttccatatg tttttggggt ttcaaaaaaa
      601 gtatgggata agaaaataaa ataaactgac caattagtgt agcatattca aaatatgccg
      661 caagtcttat tctcatatgt ggaaaccact ctattgaaaa caattctctt ccaaataaag
      721 ttggcttcag cataggaaaa aataaatata aaactgttat aagaaacaac acaaaatagt
      781 taacctttac cacattcctt acaactgaac tattaaaatc agcttttcta taataa
//
LOCUS       DATRQA010000280          825 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.282, whole genome shotgun sequence.
ACCESSION   DATRQA010000280 DATRQA010000000
VERSION     DATRQA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.282"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..254)
                     /gene="cadX"
                     /locus_tag="VBD27_002141"
     CDS             complement(<1..254)
                     /gene="cadX"
                     /locus_tag="VBD27_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEO7026088.1"
                     /translation="MKKDSICQVDVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQN"
     gene            complement(266..>825)
                     /locus_tag="VBD27_002142"
     CDS             complement(266..>825)
                     /locus_tag="VBD27_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEO7026089.1"
                     /translation="LIILMLFFAKRKSRKDIINIYLGQFLGSVSLIFLSLLFAFVLNY
                     IPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKDGLRKDNKNLIFLVAMITFASCGA
                     DNIGVFVPYFTTLNLANLIVTLLTFLVMIYLLVFSAQKLAQVPSVGETLEKYSRWFIA
                     VVYLGLGMYILIENNSFDMLWAVLG"
BASE COUNT          288 a          139 c          139 g          259 t
ORIGIN      
        1 atgttttgat tggctagttt acgaagatgg tgggaggcag atgccatact gagatttaat
       61 aaacaggcta tatcgcagac acagagttct tcgacggcaa ggagataaaa gatgatattt
      121 atctgtttat tatcggtaaa ttttgataaa atgcgaagtg atttttggac tttttccttt
      181 tcaaggtagt tcgttgcggt tgtaacattt tgttgattta taacatccac ttgacagata
      241 ctatcttttt tcataatttt ttctcctagc ctaacacagc ccatagcatg tcaaagctgt
      301 tgttttcaat caggatatac atccccaatc ctaaatagac aacggcaata aaccatctgc
      361 tatatttttc caaagtttct ccaacagaag ggacttgtgc taatttttgt gcagaaaaaa
      421 ccaagagata aatcatgact agaaaagtaa gtaaagtcac tatcaaattc gctaaattta
      481 aggtggtaaa atatgggaca aagacaccaa tattgtcagc gccacaactt gcaaaagtaa
      541 tcatagcgac tagaaaaatc aggtttttat tgtcttttcg caaaccatct tttgcaatag
      601 cttctccatc agaatctcct aaaagcaaaa cttttaggcc taggaaaatt ggaatcaaac
      661 cgagtaaacc taaaatctct ttactaggaa tataatttaa gacaaatgca aaaagcaaac
      721 ttaggaatat tagactaaca gagcctagaa attgtcctaa atagatgtta atgatgtctt
      781 ttctgctttt tcttttggca aaaaataaca ttaggataat aagta
//
LOCUS       DATRQA010000281          822 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.283, whole genome shotgun sequence.
ACCESSION   DATRQA010000281 DATRQA010000000
VERSION     DATRQA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.283"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            432..>822
                     /locus_tag="VBD27_002143"
     CDS             432..>822
                     /locus_tag="VBD27_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEO7026090.1"
                     /translation="MTFFPEINIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQSHGTPSKPVERLALNRVSAEQELEAIEQSVSMILEPEKRRILYDKYLSPYKNAD
                     KVIYTELCMSESFYYDTLDAALLAFAEL"
BASE COUNT          294 a          154 c          157 g          217 t
ORIGIN      
        1 gaaacttggc attttaaact attaaacaca gaagcaaatt aaagtcccac gcaagcgcct
       61 aagagcctgc aatggctctg tgggtctacg agctggaata ctcgttaaac ttaccctaga
      121 agctttctgt aagtattcag ctacgtagcg tggaataatc gttacgtagt tatagagcga
      181 aatttttaga aagggaaata tcctccgaca ttttttcata aaaatctaaa gtctgttatc
      241 gctcacagat gattatacaa gcgcaatgct gcaaataaag tgctgacgca aaactaaaaa
      301 tttaatactc gacaatttaa caacaaaaat aagtcagcag aggaaggaaa ggagaacaat
      361 aaaaaagccg gctcacgcaa gccagctccg atatatttgt ctactaatat tatatcatac
      421 gaggagcttt catgacgttt tttccagaaa ttaatataca aaaaactaaa tcaaacgcta
      481 agcgaaaact aagagagtat ccacgctggc gtaggatcgc taatgatgta gatactcaaa
      541 aagtgacagc cacttattcc tttgaaccta gacaatcaca tggaacccct agtaagccgg
      601 ttgaacgctt agcactcaat cgtgtgtcag cggaacaaga attagaagca attgagcaat
      661 cggttagtat gatactagag ccagaaaagc gcaggatttt gtatgacaaa tacttatctc
      721 cttacaagaa tgcagataag gttatttata cagaattatg tatgtcagag agcttttatt
      781 atgacacgct agatgctgca ttattagctt ttgcagagct tt
//
LOCUS       DATRQA010000282          818 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.284, whole genome shotgun sequence.
ACCESSION   DATRQA010000282 DATRQA010000000
VERSION     DATRQA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.284"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(18..347)
                     /locus_tag="VBD27_002144"
     CDS             complement(18..347)
                     /locus_tag="VBD27_002144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026091.1"
                     /translation="MGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQT
                     PPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKR
                     IFQKLEI"
     gene            complement(326..649)
                     /locus_tag="VBD27_002145"
     CDS             complement(326..649)
                     /locus_tag="VBD27_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO7026092.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYLW
                     EFRYF"
BASE COUNT          273 a          167 c          128 g          250 t
ORIGIN      
        1 ctatttaacc ccttgtatta tatctctaac ttttgaaaaa ttctttttga atatattgaa
       61 atcatgattg aagcaataga agctacaaac ataacaaccc atgtttcttg tataaataca
      121 tcgaataaaa taccgtaaac catttgtcca ataggttgac cgcacatagc aactgctacc
      181 agcgtagcca ttatctttcc tactaattgc ggtggtgttt gtgtctgaac aagagtgtaa
      241 atttgaacaa caaacatagt agatacaccc atagccacaa agcacatcac tgtaactacc
      301 cagtaggaaa ctattgcagg caattctaaa aatagcgaaa ttcccataag taaaacagca
      361 gtagaatttc acgtttcttg tctggcaact cacgtaatgc ttcactcaac aaatcatttt
      421 caacgcctac tgataaccca ttgagtgtaa aaatctgaaa gtcagttgaa tagttatctg
      481 ttgtcgcaaa ctggctaaca agataatcgc caacatccga aaaggacacc tcacgctttg
      541 caatccttga aagataaagc atataattct ttcgctcgtc ttccatagca cgtttacaga
      601 tatagtcaaa ctgattttct attgtggtct gaaaagaaga tggtttcatg tttctcaccc
      661 cctttctgtc taggaaagga agtgagcctt gctcgtttat ctcctttcac tcttagtccc
      721 aatgtgaaag ggggatttgt tgcattactg ataaataaac tttgtaaaaa agttctgaat
      781 agccaaaaaa gcatataaac agatttattt ctctgttt
//
LOCUS       DATRQA010000283          796 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.285, whole genome shotgun sequence.
ACCESSION   DATRQA010000283 DATRQA010000000
VERSION     DATRQA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.285"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            1..796
                     /locus_tag="VBD27_002146"
                     /pseudo
     CDS             1..796
                     /locus_tag="VBD27_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISSag5 family transposase"
BASE COUNT          298 a          158 c          121 g          219 t
ORIGIN      
        1 tacttactgc caagcaaata caagagttac aaaaacgtaa tgcgcagctt gaagaggaga
       61 accttatcct aaaaaaagca agtgccatat tcatgcaaaa cttaaagtaa gactcctcgc
      121 tgtctatcgg ttacgctttg aacacgccac aacaaccttg tgtcgtgttt tacgtgtcaa
      181 tcgctccact tactataaat ttctaaaaca taagccctca aaaagagaat tggataatca
      241 aatttataaa aaacaaatac ttgagattta taccaaagca aacaaaagac ttggtgtgaa
      301 gtctatcaaa gtcattcttc aaagagacta cgacacaaaa atctctgaag gaagaattta
      361 ccatctgatg aagaatatgg cgctccctaa aatggctacc gttaagccaa aaacagctct
      421 taaaaagact caaaaaacgt atcctcaaaa cttactcaac cagaaatttt atcctgacaa
      481 acctaatcaa gtatggtcta ctgacttcac ctatatttct attggatata agaaatatgt
      541 ctatctctgc gcaatacttg atctctattc tagaaaatgt attgcttgga aattgagtca
      601 ccgtatggat gcaaagttag catgtaacac tctagaatta gctcttaata aaagaaagat
      661 tgaaggaaca cttctctttc attccgacca agggtcacaa tttaaggcca gggaatttag
      721 aaaaataatt gatgacaaca atatcatgca ttctttttct aaacctggat atccttatga
      781 taatgccgta actgaa
//
LOCUS       DATRQA010000284          789 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.286, whole genome shotgun sequence.
ACCESSION   DATRQA010000284 DATRQA010000000
VERSION     DATRQA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.286"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            193..602
                     /locus_tag="VBD27_002147"
                     /pseudo
     CDS             193..602
                     /locus_tag="VBD27_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            677..>789
                     /locus_tag="VBD27_002148"
     CDS             677..>789
                     /locus_tag="VBD27_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7026093.1"
                     /translation="MGDVVENFTEGKNPKIDTLNGKTVRIEKINPDHFEDL"
BASE COUNT          323 a           74 c          147 g          245 t
ORIGIN      
        1 agattattga agtggtttat agataattaa ggaaggaata ttgattagaa atgaataatt
       61 tggatacact atattgtaat ttagcaatgt gcaatcatag tactaatgga aaggtagcga
      121 cttcagcttg ttgatttatc taatgtaaat gagtttttag aattttcatc tgattttgaa
      181 actgtatttc atatgcaacg atataaagcc aataccacag aagaagcaca ttgattttgg
      241 cgaatgtctg tatgaaatca ttattaggaa tctatgcgat gattgaaaca gaatcaaata
      301 aaatgattgg tattatagaa ttagaaatta gagacgagtt tccagcagaa ttcggttata
      361 tattaaataa aaattatagt ggaaaaggtt atatgataaa agcttgttca aaattgatgt
      421 caattggatt tgaacaacta gatttggaaa gaatttatgc tcgatttgaa ctaataataa
      481 aaaatctgaa aatgttatgg aatgcctaag aatgcaaaaa gaaggtgaac gtcgtcatat
      541 tgtaaaaaaa tccaaaaggt gagtggaaaa ccagagcata ttattctatt ttaaaggaat
      601 aatattttaa atagacgaca ataaaaattt gtgaaagcag aaaaattaaa tgagatacaa
      661 taggtttaat caactagtgg gtgatgtcgt tgaaaatttt acggaaggta aaaatccgaa
      721 gattgataca cttaatggga aaacagttag aatagaaaaa ataaatccag atcattttga
      781 agacttatt
//
LOCUS       DATRQA010000285          769 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.287, whole genome shotgun sequence.
ACCESSION   DATRQA010000285 DATRQA010000000
VERSION     DATRQA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.287"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            174..449
                     /locus_tag="VBD27_002149"
     CDS             174..449
                     /locus_tag="VBD27_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026094.1"
                     /translation="MKKNSLYNQLINSEPIGFIDPLTDLGEFDIFQMKFKEPVRYLKN
                     KYSGKPYSPHWQNKIEKMRSHQESLREEVKEEIPITSRARNQENNLS"
     gene            510..769
                     /locus_tag="VBD27_002150"
                     /pseudo
     CDS             510..769
                     /locus_tag="VBD27_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
BASE COUNT          310 a           91 c          138 g          230 t
ORIGIN      
        1 agatcataga aaggtgaaga gaacatggaa ggtattgaaa taatattaga tatagaaacg
       61 ttaggaattt ttcaagtcat agatcatggt atctctccag atgatagaga aacatatgaa
      121 caattaactt tgttttaaat aacttaactt tattatctta gaggatgtta catatgaaaa
      181 agaatagttt atataatcag ttaataaata gtgaaccgat aggctttatt gatccactaa
      241 ccgacttggg ggaatttgat atttttcaaa tgaagttcaa agaacccgtt agatacttga
      301 aaaataaata ttcaggaaag ccttattcac cacattggca gaataaaatt gaaaaaatga
      361 ggtctcatca agaaagccta agagaagaag ttaaagaaga gattccgatt actagtaggg
      421 caagaaatca agaaaataat ttgagttgat cttaaattga taaaaagttc gacttttttc
      481 ctaaaaaata gtgtgctata agtcaattaa aagatgtgct atatttattt aatagatatc
      541 gtctcattga atatcaacta accggagaaa attcgaatgc aaaaaattta atgacaggca
      601 ctcaaaattt aaatgctaat tttgaagatg aaaaatcgtc gatggtatat tatgaaaata
      661 tggtggctca ttatataaga ctaacaaacc atcatgtgag ataccgtgtt acactcttat
      721 ttaaaaatat tgagatggtt gcaagagggg ttggtatgga aacccaaag
//
LOCUS       DATRQA010000286          766 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.288, whole genome shotgun sequence.
ACCESSION   DATRQA010000286 DATRQA010000000
VERSION     DATRQA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.288"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..232)
                     /locus_tag="VBD27_002151"
     CDS             complement(<1..232)
                     /locus_tag="VBD27_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO7026095.1"
                     /translation="MLHLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFTFIFL
                     IYQYDRETIFKTITIVIFFLFYFLWQNHNMNVQ"
     gene            complement(216..>766)
                     /locus_tag="VBD27_002152"
     CDS             complement(216..>766)
                     /locus_tag="VBD27_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO7026096.1"
                     /translation="THHKEDDLNINLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKG
                     AVNHPGVYSLPSQSRVTDAIKRAGGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITV
                     VEEEKANNIATQGNSKGKINLNKADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDD
                     LKNVSGIGEKTLEKLRQDVTLD"
BASE COUNT          248 a          136 c          108 g          274 t
ORIGIN      
        1 attggacatt catattgtga ttttgccata aaaaataaaa taagaaaaaa attactattg
       61 ttattgtttt aaaaatagtt tcacgatcat attgataaat cagaaaaata aaagtaaata
      121 aaagaaaggc acaaccaagc attgtccaag aaaacactag taagtaaatt tggaagacca
      181 acaatgctaa ataaataggt tttagaggaa aatacttagt caagtgtaac atcttgacgt
      241 aatttttcta gtgttttctc accaatacct gatacatttt tgaggtcatc aatagtttta
      301 aaaccacctt gactatcacg ataatctaaa atatcttgag ctcttttagc tccaacgcct
      361 gatatggtct gaagacttga taaatctgct ttattaagat taattttccc cttactattt
      421 ccctgagtag caatattatt tgctttctcc tcttccacaa cagttatttt ttcacctttt
      481 tgagcaacat agataaccgt ctcatcttgt aatttctgtg ctagattaac ggatttacta
      541 tcagcgagat tgcttagtcc acctgcacgc ttaatcgcat ctgttacacg actttgagaa
      601 ggcaaactat aaacacctgg atgattgact gcccctttta cgtcaaccgt tacttgatta
      661 gatactttat ctttgacgtg actaatcatt ggtacctttt tttctgttat agttgatact
      721 tttttctcta aattgatatt taaatcgtct tctttatgat gagtgc
//
LOCUS       DATRQA010000287          761 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.289, whole genome shotgun sequence.
ACCESSION   DATRQA010000287 DATRQA010000000
VERSION     DATRQA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.289"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..212
                     /locus_tag="VBD27_002153"
     CDS             <1..212
                     /locus_tag="VBD27_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7026097.1"
                     /translation="YPYDNAVTEAFFKYLKHRQINQKNYQNIKQVQLDCFEYIENFYN
                     NYIPHTANLGLTPNQKEENYFNAIK"
     gene            complement(333..665)
                     /locus_tag="VBD27_002154"
     CDS             complement(333..665)
                     /locus_tag="VBD27_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO7026098.1"
                     /translation="MLRKYKQRNYNVAHTKQRFSIKKFKFGVASVLIGLTFLGMSSHS
                     VLADESSIMSTEVPQTAGVKPEVTVTQPTSDVTSLATDTSTSAVLSTDASHSNPTESA
                     EHAVGLEC"
BASE COUNT          246 a          147 c          111 g          257 t
ORIGIN      
        1 gatatcctta tgataatgcc gtaactgaag catttttcaa gtatttaaag catagacaaa
       61 tcaaccaaaa aaattatcaa aatatcaaac aggttcaatt agactgcttt gaatacattg
      121 agaattttta taacaattac atcccacata cggctaatct aggactaacc cctaatcaga
      181 aagaagaaaa ttattttaac gcaataaaat aacacggttt tctgtctact tatttgacat
      241 tagtccactc tgtgaattca gatgtggaag aagaagtaac tcgcaatgtt ttatttgctt
      301 ctttttcgtg tgtcactgga accgctgcat cttcagcatt ctaatcctac agcatgctcc
      361 gctgattctg taggattaga atgagatgca tctgttgata acactgctga agttgacgta
      421 tctgtcgcaa gacttgttac atctgaagta ggctgtgtaa cagtaacttc cggttttaca
      481 ccagctgttt gtggtacctc agtactcata atgcttgact catccgccaa aacagaatga
      541 ctactcattc ctagaaatgt caatccaatt aataccgatg ctacaccaaa tttgaatttc
      601 ttaatcgaaa aacgctgttt cgtatgtgcg acattataat ttctttgctt atacttccta
      661 agcataactt ctctcctttt caaaagtaaa aatagattaa ccaataaagt tatctatttt
      721 tacttttttt aaagttttac tgttttacat tatctcgata a
//
LOCUS       DATRQA010000288          711 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.290, whole genome shotgun sequence.
ACCESSION   DATRQA010000288 DATRQA010000000
VERSION     DATRQA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.290"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..366
                     /locus_tag="VBD27_002155"
     CDS             <1..366
                     /locus_tag="VBD27_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO7026099.1"
                     /translation="REESGKIEPWFVIIPDMNSFVAETNMDKEQILFLLDSAHKYHIY
                     FLFGGLASYLMTNISDVPKAIRTQAQYVLLGMRVMDQSVLPKTYNSKEPYLKPDMIYI
                     HDRRQETMLKITQEIEMEH"
     gene            371..>711
                     /locus_tag="VBD27_002156"
     CDS             371..>711
                     /locus_tag="VBD27_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026100.1"
                     /translation="MKKQRLLLLFGGLLIMIMMTACKDSKIPENRTKKEYQAEQNFKS
                     YFKYISDKNNYLDNIKVYYFSISISKDVQDKVSETTTCSYRLEKQKNQEFIGNFEHEV
                     SESSQYSTEVK"
BASE COUNT          287 a           95 c          117 g          212 t
ORIGIN      
        1 agagaagaat ctggtaaaat agaaccatgg tttgttatta ttcctgatat gaatagtttt
       61 gtggcagaaa caaatatgga taaagaacag attttattct tacttgactc ggctcacaaa
      121 tatcatattt atttcttatt tggtggctta gcatcatacc ttatgactaa tatctcagat
      181 gttccaaaag ctattagaac acaggcacag tatgttctct tgggaatgag agttatggat
      241 cagtcggtat taccgaaaac atataattca aaagaacctt atttgaaacc agatatgatt
      301 tatattcatg atagaagaca agagacaatg cttaaaatca ctcaagaaat agaaatggag
      361 cattgagcat atgaaaaaac aaagactatt actgcttttt ggaggcttat taataatgat
      421 aatgatgaca gcatgtaagg attcaaaaat cccagaaaac cgcacgaaaa aggaatacca
      481 ggcagaacag aattttaagt catactttaa atatatatca gataaaaata actatttaga
      541 taatataaaa gtttattact tttctataag tatttctaaa gatgtacaag ataaagtcag
      601 tgaaacaaca acttgttcat atagactaga aaagcaaaag aatcaagagt tcattggtaa
      661 ttttgaacat gaagttagtg aatctagtca atattcaacc gaagttaaaa a
//
LOCUS       DATRQA010000289          709 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.291, whole genome shotgun sequence.
ACCESSION   DATRQA010000289 DATRQA010000000
VERSION     DATRQA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.291"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            54..344
                     /locus_tag="VBD27_002157"
     CDS             54..344
                     /locus_tag="VBD27_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO7026101.1"
                     /translation="MSQIKLTPEELRISAQKYTTGSQSITDVLTVLTQEQAVIDENWD
                     GTAFDSFEAQFNELSPKITQFAQLLEDINQQLLKVADVVEQTDSDIASQINK"
     gene            415..>709
                     /locus_tag="VBD27_002158"
     CDS             415..>709
                     /locus_tag="VBD27_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026102.1"
                     /translation="MKKSTQIILLIVALFILVFSGGFYMKEQQRKEELKRNREYEVSL
                     VKALKNSYENIEEIKITHPVSTEIPGDWHCTVKISFNDKKSIVYNITHNLESKK"
BASE COUNT          298 a           96 c          110 g          205 t
ORIGIN      
        1 gtaaggaaca tctaaatatg ctatactgca gttaaaaaaa tagggagcaa atgatgtcac
       61 aaattaaact aacacctgaa gaactccgta tttcagcaca aaaatataca acaggatcac
      121 aatcaattac agatgtgtta acagttttga ctcaagaaca agctgttatt gatgaaaatt
      181 gggatggtac agcatttgat agctttgaag cccaattcaa tgaattatct ccaaaaatca
      241 cacaatttgc acaattatta gaagatataa atcaacaatt attgaaagtt gcggatgttg
      301 tcgaacaaac agactcagat attgcctcac aaattaataa ataattggct tattcaaatt
      361 cataagttga tatattatag ctagatgatg taattgtttc ataaaggagt aaaaatgaaa
      421 aagagtaccc aaataatact actaatagtt gcattattca tacttgtttt tagcggagga
      481 ttttatatga aagaacaaca aagaaaagaa gaactaaaac ggaatcgaga atatgaagtt
      541 agtctagtca aagcattgaa aaattcctat gagaatatag aagaaataaa aatcacacat
      601 cctgtttcaa ctgaaattcc tggagattgg cattgtactg taaagatttc atttaatgat
      661 aaaaaatcta ttgtttataa tattacacat aatttggaat cgaaaaaaa
//
LOCUS       DATRQA010000290          704 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.292, whole genome shotgun sequence.
ACCESSION   DATRQA010000290 DATRQA010000000
VERSION     DATRQA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.292"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            153..653
                     /locus_tag="VBD27_002159"
     CDS             153..653
                     /locus_tag="VBD27_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7026103.1"
                     /translation="MACIIRRARLGDEVNLAYIQTESWKAALGKILPEDIIQKTTEIE
                     PAITMYQQLLHKEVGKGYILEVDSNPHCMAWWDKSREDGMLDYAELICIHSLKEGWGK
                     GYGSQMMNHVLSEIQQAGYNKVILWVFTENTRARKFYDRFGFSFKGKSKTYFEKEEMC
                     YEKILH"
BASE COUNT          269 a           80 c          149 g          206 t
ORIGIN      
        1 tctgaggtta atgtttaaaa ccatctaaaa ttttagaaat ataatgataa atgagaacca
       61 gaactgttca aaataaaaat taatatcacg aaaatggtat aattggagta gttatcaggc
      121 agttaataat aatttttgaa aggattttag ttatggcttg cataatcagg agagcaagat
      181 taggagatga agtcaattta gcttatattc aaacggagag ctggaaagct gctttgggta
      241 agatactacc tgaggatatc attcaaaaaa ctacggaaat agaacctgcc ataacaatgt
      301 atcaacagct actacacaag gaggttggta aggggtatat attggaagta gatagcaatc
      361 ctcattgtat ggcttggtgg gataaaagta gagaagatgg tatgcttgat tatgcagaac
      421 tcatttgtat acatagtctt aaagaaggtt ggggaaaagg ttatgggtct caaatgatga
      481 atcatgtact atcagagata caacaagcag gttataataa agtgatactt tgggtattta
      541 cagaaaatac acgcgcaagg aaattttacg ataggtttgg tttttctttt aagggtaaat
      601 caaaaactta ttttgaaaag gaagaaatgt gttatgagaa gattttgcac tagcgagata
      661 aagcatccga taatacaata gtataaacat cctattttga aaaa
//
LOCUS       DATRQA010000291          701 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.293, whole genome shotgun sequence.
ACCESSION   DATRQA010000291 DATRQA010000000
VERSION     DATRQA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.293"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            172..423
                     /locus_tag="VBD27_002160"
     CDS             172..423
                     /locus_tag="VBD27_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026104.1"
                     /translation="MIINSFDNSRKATINPEDINSPIKGFPKTVITCFARETFNRILE
                     ELPHREIARTSVANLEIPIYELEFKGQKIVLIKRLVMKI"
     gene            420..>701
                     /locus_tag="VBD27_002161"
     CDS             420..>701
                     /locus_tag="VBD27_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7026105.1"
                     /translation="MICLHGMNWVDGQLAAYYRLIPEDDKVHLGRVIVNPDFRKKGLG
                     NQLVEYEIKFSEANYPNKPIYAQAQAYLQDFYQSFGFQPISDIYLEDNIP"
BASE COUNT          246 a           91 c          128 g          236 t
ORIGIN      
        1 gtttaagcta tttgatacgg aaatttacct tagaaatggt aaataattcc attaagtata
       61 ttcatctttg caaatttgac cttgcctttt aagtttcatt gggttaaaat agaaaaaata
      121 tttttaacaa gatgatagaa catcgtaaat taaggtttaa aggaaatgct tatgataata
      181 aattcattcg ataatagtag aaaagcgact attaatcctg aagatattaa ttcaccaata
      241 aaaggttttc caaagactgt tattacctgt tttgcgcggg agactttcaa tcgcatttta
      301 gaagaattgc ctcatcgtga aattgctaga actagtgtag caaacttaga aattcccatt
      361 tatgaattag aatttaaagg acaaaagatt gtccttatca agaggttggt gatgaagatt
      421 tgatttgttt gcatgggatg aattgggtag atggtcaatt ggcagcttat tatagattga
      481 taccagaaga tgataaggtg catttgggac gtgtgattgt taatccagac tttagaaaaa
      541 agggattagg aaatcaatta gtggaatatg agattaaatt tagcgaggca aattacccta
      601 ataaacctat ttatgctcag gcccaagctt atttacaaga tttttatcaa tctttcggat
      661 ttcaacctat ctcagatatc tatctagaag ataatattcc t
//
LOCUS       DATRQA010000292          687 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.294, whole genome shotgun sequence.
ACCESSION   DATRQA010000292 DATRQA010000000
VERSION     DATRQA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.294"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            180..>687
                     /locus_tag="VBD27_002162"
     CDS             180..>687
                     /locus_tag="VBD27_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7026106.1"
                     /translation="MVTIKDVAAKAGVNPSTVSRVLKDNESISSKTKERVKKAMEELG
                     YVPNVAAQMLASGLTQNIGVIFPPLVLPDRLSEPFFMEILSTLTNEAKEHGFTVSIAT
                     SMSSEELLEQVKLMHLQKRVDGFIVLYSDQKDPIREYLLKYKLPFVVVGAANGYENEI
                     TYIDNDNQL"
BASE COUNT          248 a           87 c          137 g          215 t
ORIGIN      
        1 ttaaaaaaaa taagaaaaat aaaaaaataa tatcaattaa tactaaatga gaagattatt
       61 ttagtgagag tattacattt gcagagaagg caaacgtttg tttttttctt agattagagt
      121 ataatagaca aaggactata ccaaaaggta taaaatgatt agaatgaaga ggtcgtagga
      181 tggtaactat aaaagatgtt gctgccaaag ctggtgttaa tccatcgaca gtaagtcgtg
      241 tactaaaaga taatgaatct atttcaagta agacgaaaga gcgcgtgaaa aaagctatgg
      301 aagagctggg ctatgttcct aatgttgcag cacaaatgtt ggctagtggt ttaacgcaaa
      361 atattggagt tatttttcct ccactggttt taccagaccg cttaagtgaa ccctttttca
      421 tggagattct ttctacatta actaatgaag caaaagagca tgggtttaca gtttctattg
      481 caacaagtat gtcttctgag gagttgttag aacaagtaaa attaatgcat ctacaaaaac
      541 gagtggatgg ttttattgtt ctctattcgg atcaaaaaga tcctatcaga gagtatttat
      601 taaaatataa actgcccttt gttgttgttg gtgctgctaa tggctatgaa aatgagatta
      661 cttatattga taatgataat cagctga
//
LOCUS       DATRQA010000293          677 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.295, whole genome shotgun sequence.
ACCESSION   DATRQA010000293 DATRQA010000000
VERSION     DATRQA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.295"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..469
                     /locus_tag="VBD27_002163"
     CDS             <1..469
                     /locus_tag="VBD27_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEO7026107.1"
                     /translation="EKLKLKQIPTLNNTKFALIEFSMQTSWKDIHTALSNVLMLGITP
                     VVAHIERYNALENQKERVKEIINMGCYTQINSFHILKQKLFNDKHKRFKKRARYFLEE
                     NLVHFVASDMHNLDVRPPFLAEAYKIICRDFGKERANQLFIENAQSILKNHYI"
     gene            478..>677
                     /locus_tag="VBD27_002164"
     CDS             478..>677
                     /locus_tag="VBD27_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEO7026108.1"
                     /translation="MNKIANTEVEINIFNLLKKLWKKKFLITFVAIAFATAGLFYSLF
                     IVTPQYTSSTRIYVINPNTPNN"
BASE COUNT          251 a           97 c          105 g          224 t
ORIGIN      
        1 agaaaaactg aagttaaagc aaattccaac tttaaacaat acgaaatttg ctttaattga
       61 attttctatg caaacttctt ggaaagatat tcatacagct ttgtcaaatg ttttaatgct
      121 tggtattaca ccagtcgttg cgcatataga gaggtataac gctttagaga atcaaaaaga
      181 acgggtgaag gaaattatta atatggggtg ttacacacaa ataaatagtt tccatatttt
      241 gaaacaaaaa ctttttaatg ataagcataa acgctttaag aaaagagccc gttatttttt
      301 agaggaaaat ttagtgcatt ttgtagcgag tgatatgcat aaccttgatg ttagaccgcc
      361 atttttagca gaagcttata agattatctg tagagatttc ggtaaagaac gtgctaacca
      421 actttttatt gaaaatgctc aatctatatt aaaaaaccat tacatttagg agatttcatg
      481 aataaaatag ctaatacaga agttgaaata aatatattca accttttaaa aaaattgtgg
      541 aaaaagaaat ttttaatcac atttgttgcc attgcgtttg caacagctgg acttttctat
      601 agtcttttta ttgttacgcc acaatatact tcttcaacaa ggatatatgt cattaaccct
      661 aataccccta ataatag
//
LOCUS       DATRQA010000294          675 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.296, whole genome shotgun sequence.
ACCESSION   DATRQA010000294 DATRQA010000000
VERSION     DATRQA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.296"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..>675)
                     /locus_tag="VBD27_002165"
     CDS             complement(<1..>675)
                     /locus_tag="VBD27_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein"
                     /protein_id="HEO7026109.1"
                     /translation="RSKNNSYDTLQTKQRFSIKKFKFGAASVLIGISFLGGFTQGQFN
                     ISTDTVFAAEVISGSAVTLNTNMTKNVQNGRAYIDLYDVKNGKIDPLQLITLNSPDLK
                     AQYVIRQGGNYFTQPSELTTVGAASINYTVLKTDGSPHTKPDGQVDIINVSLTIYNSS
                     ALRDKIDEVKKKAEDPKWDEGSRDKVLISLDDIKTDIDNNPKTQSDIANKITEVTNLE
                     KILVPRI"
BASE COUNT          206 a          128 c           91 g          250 t
ORIGIN      
        1 gattcgaggt actagtattt tttctaaatt agtaacttca gttattttat tggcaatgtc
       61 tgattgcgtc ttaggattat tatcaatatc tgttttgata tcatctaaac ttatcaaaac
      121 tttatcgcga cttccctcgt cccatttagg gtcttccgct ttctttttaa cttcatctat
      181 tttatctctc aaagctgaag aattgtaaat agtcaatgaa acgtttataa tatccacttg
      241 tccatcaggc ttcgtatgag gacttccatc tgtcttcaat actgtataat taatactagc
      301 tgcaccaaca gtagtcaatt cagaaggttg tgtgaaataa ttgccgcctt gcctaatgac
      361 atactgagct tttaaatcag gtgaatttaa cgtaattaat tgtaatggat ctattttccc
      421 atttttcaca tcatataaat ctatatatgc tctaccattc tgaacatttt tagtcatatt
      481 tgtgtttaac gtaacagcac ttcctgaaat tacttcagct gcaaacacag tatctgtaga
      541 aatattaaat tgcccttgag taaaacctcc taaaaaacta ataccaatta gtacagaagc
      601 tgcaccaaac ttaaacttct taattgaaaa ccgttgtttc gtctgtaaag tatcataact
      661 gttattttta gacct
//
LOCUS       DATRQA010000295          672 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.297, whole genome shotgun sequence.
ACCESSION   DATRQA010000295 DATRQA010000000
VERSION     DATRQA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.297"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..199)
                     /locus_tag="VBD27_002166"
     CDS             complement(<1..199)
                     /locus_tag="VBD27_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026110.1"
                     /translation="MTMKTIEEIYKNYLNIPYISPERDLAEINFSKVVPRKNMEETSE
                     GLLPGDIILLWRIQFGTFTTET"
     gene            complement(250..>672)
                     /locus_tag="VBD27_002167"
     CDS             complement(250..>672)
                     /locus_tag="VBD27_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEO7026111.1"
                     /translation="LFAVGQVVAGVLLYYFKPTQIKHLLFLLTEGEVHGGIHGFISAH
                     FLKISSYINDSASHLAAIYLLIHGGIKLVALILLHKEKLFAYPLSILVFVGFIIFQLR
                     EYFSSHSILMIFLCVFDVIMIILTWLEYQKMKLNFSKR"
BASE COUNT          256 a          106 c           99 g          211 t
ORIGIN      
        1 ttgtttcagt tgtgaaagtt ccaaactgta tacgccataa taagataata tctccaggca
       61 agagtccttc actagtttct tccatatttt tcctaggaac aactttacta aaattaattt
      121 cagccaagtc tctttcaggt gagatatagg ggatattaag ataatttttg taaatttctt
      181 caatagtttt catagtcata ttgtagcaaa aaagcgctta taaaaacaag cgctaaggca
      241 tgggggaagt cacctttttg aaaaattcaa tttcattttc tgatattcta accatgttaa
      301 aattatcatg ataacatcaa atacacatag aaaaatcatt aaaatgctat gactagaaaa
      361 atattccctt agttggaaaa tgataaaccc aacgaaaact aaaattgata aagggtaagc
      421 aaataatttc tctttatgta ataaaataag tgctactaat ttgataccgc catgaattaa
      481 tagatagata gcggctaaat gactagccga atcattaata taactgctta ttttcaagaa
      541 atgagcagaa ataaaaccat gaatcccacc gtgaacttca ccttcagtta aaagaaatag
      601 gagatgtttt atttgtgtag gtttaaaata atataataaa acgccagcta ctacttgtcc
      661 gactgcaaat ag
//
LOCUS       DATRQA010000296          634 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.298, whole genome shotgun sequence.
ACCESSION   DATRQA010000296 DATRQA010000000
VERSION     DATRQA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.298"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(130..>634)
                     /locus_tag="VBD27_002168"
     CDS             complement(130..>634)
                     /locus_tag="VBD27_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO7026112.1"
                     /translation="YNFNKLMIYLTHPFISDLNMPSFPSSEDGLKHFADVKYLFTLAH
                     GLFVILTFPVIYFLRRGWKQKSIFLYEGFFKIAIMLPIFIVVCAFLLGFDQFFTLFHE
                     VLFPGDSTWQFNPLTDPVIWILPETFFLHCFIIFLLIYETITIILLIIGRKHLKLRKY
                     KNKMQAL"
BASE COUNT          253 a           95 c           93 g          193 t
ORIGIN      
        1 ttttaatcca gtccgtattt agcttgaaaa agtgagcacc gattcggtgc tttttttatg
       61 tcattgttat aattccgaca tttctcatat ttgtattata aaaaaatagc aaacaaaatg
      121 caagcgctat tataaggctt gcattttgtt tttatatttt cttaatttta aatgttttct
      181 acctattatc aaaagaatta tagttattgt ttcatagatt aacaaaaaaa tgatgaaaca
      241 atggaggaaa aaagtttcag gaagtatcca aattactgga tccgttagag gattaaattg
      301 ccaagtagaa tctcctggaa aaagcacctc atgaaataga gtgaaaaact gatcaaaacc
      361 caacagaaaa gcacatacta caataaaaat aggcaacata atagctatct tgaagaagcc
      421 ttcatataga aagatacttt tttgcttcca tccacgtctt aaaaaataaa taactggaaa
      481 agttagtata acaaaaagcc catgcgctag agtaaaaaga tattttacat ccgcaaaatg
      541 ctttaaacca tcctctgaag aaggaaagct aggcatattt aaatcagaga taaaaggatg
      601 agtcagatat atcatcaact tattaaaatt ataa
//
LOCUS       DATRQA010000297          624 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.299, whole genome shotgun sequence.
ACCESSION   DATRQA010000297 DATRQA010000000
VERSION     DATRQA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.299"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            51..>624
                     /locus_tag="VBD27_002169"
     CDS             51..>624
                     /locus_tag="VBD27_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO7026113.1"
                     /translation="MNHQILQNYINSHNLPIIEKDYHKYLTFESLEEDYTYILKDGIV
                     KQSVLSKYGTEFNLRYVTGLEITSILNTDYSQHMGEPYNVRIESETAHFYKVRRSTFL
                     KDINNDIELQGYVKDFYHNRLEKSMKKMQCMLTNGRIGAISTQLYDLSKMFGEERDNG
                     DIYINFVITNEELGKFCGISTGSSVSRILKQ"
BASE COUNT          228 a          111 c          101 g          184 t
ORIGIN      
        1 ttaacaaatg ttaaggtggt ctatcaaacc taaaacattt ggaggaagtc atgaatcatc
       61 aaattttaca aaattacatt aatagtcata atcttccaat tatcgaaaaa gactaccata
      121 aataccttac atttgaaagt ttagaagaag attatactta tatcctaaaa gatggtattg
      181 ttaaacaaag tgttctctct aaatatggta ctgagttcaa tctgcgttat gtgacaggct
      241 tagaaatcac ttctatccta aatacagact actcccaaca catgggagaa ccttacaacg
      301 ttcgaatcga atccgaaact gcccactttt acaaagtacg tcgctctacc ttcctaaaag
      361 atatcaataa tgatattgaa ctgcaaggat atgtgaaaga tttctatcat aaccgccttg
      421 aaaaatctat gaaaaaaatg caatgtatgc tcactaatgg tcgaattggt gctatctcca
      481 cacaattata tgacctcagt aagatgtttg gtgaagaacg agataacggt gatatctata
      541 ttaattttgt tatcacgaac gaagaacttg gtaaattttg cggaatatcc acaggaagta
      601 gcgtgagccg cattttgaaa caac
//
LOCUS       DATRQA010000298          620 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.300, whole genome shotgun sequence.
ACCESSION   DATRQA010000298 DATRQA010000000
VERSION     DATRQA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.300"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(28..342)
                     /locus_tag="VBD27_002170"
     CDS             complement(28..342)
                     /locus_tag="VBD27_002170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026114.1"
                     /translation="MEEMYNNGIDFYQDNIETEIQFLVSKGIIAPDPNFYCHQFIPSI
                     KKFSLENDETVQKLLKENEYSTPKTIISVEHKVYCLTTLGNELKDTLINGSDILFTED
                     DL"
     gene            complement(273..>620)
                     /locus_tag="VBD27_002171"
     CDS             complement(273..>620)
                     /locus_tag="VBD27_002171"
                     /inference="COORDINATES: protein motif:HMM:NF025695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4393 domain-containing protein"
                     /protein_id="HEO7026115.1"
                     /translation="FCNLFASLIASSMDKRKTVKPIYSSILKEMSSSDAKLLQYFFSN
                     RFIFEINIQSSNHLFSIQIYITGIGIVNIIKGSILIILKQLGIMNNPQKWKKCTITAL
                     IFTKIILKPKFNF"
BASE COUNT          218 a           80 c           88 g          234 t
ORIGIN      
        1 attctgaaga tattataaaa agtgtaatta aagatcgtct tcggtaaata aaatatcact
       61 tccattaata agagtatctt ttaattcatt ccctaatgtc gtaagacaat atactttatg
      121 ctcaactgaa ataatcgtct tcggtgtgga atattcgttt tctttaagta atttctgaac
      181 agtttcatca ttttcaagtg aaaatttttt aatgcttgga ataaattggt gacaataaaa
      241 attgggatca ggtgcaatta ttcctttact aactaaaaat tgaatttcgg tttcaatatt
      301 atcttggtaa aaatcaatgc cgttattgta catttcttcc atttctgtgg gttgttcata
      361 attccgagtt gcttcaaaat tatgaggata gatcccttta taatatttac tatcccaata
      421 ccagttatat aaatttggat tgaaaataaa tgattactac tttgaatatt aatttcaaat
      481 ataaaacgat tcgaaaaaaa gtattgtaat agttttgcat ctgaagacga catttctttt
      541 agtatagaag aataaatagg tttgacagtt tttcttttat ccatacttga tgcaataagt
      601 gaagcgaata aattgcaaaa
//
LOCUS       DATRQA010000299          594 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.303, whole genome shotgun sequence.
ACCESSION   DATRQA010000299 DATRQA010000000
VERSION     DATRQA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.303"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            116..>594
                     /locus_tag="VBD27_002172"
     CDS             116..>594
                     /locus_tag="VBD27_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026116.1"
                     /translation="MRETETHDHQALIQKLLVSIHYLTLFRDEIILVEKTPSLLGKHF
                     SIAIVQNELGEILSKIEALSKQKKLIRSIYWYDESSFKVMNKALAIVEEWIKGLDNLL
                     EFCQSQTVFQAILGDERAHVFGILIDVYTSLNIINTSLKEENSRPVSLSDLALGLKD"
BASE COUNT          199 a           77 c          106 g          212 t
ORIGIN      
        1 aatatttatt ttataaaatt attcatctta tttttgcgat ttcaatagtg atatggtata
       61 tttaaagtaa gctacgtata gttttaccag tcagaaatag taaggatggt tgagtatgag
      121 agaaactgaa acacacgatc accaagcgct aattcaaaaa ttgttagtca gtattcatta
      181 tttaaccttg tttcgtgatg aaattatatt agtagaaaaa accccatctt tactggggaa
      241 acatttttct attgctattg tgcagaatga actaggtgag atactttcta aaatagaagc
      301 tttgagtaag caaaaaaaac tgattcgttc tatttattgg tatgatgaat cgtcatttaa
      361 agtcatgaat aaggctctgg ctattgtaga agaatggatt aagggcttag ataatctttt
      421 agaattctgt caatcacaga cggtttttca agcaatttta ggtgatgaac gtgcacatgt
      481 atttggaatc cttattgatg tttatacttc tttaaacatt ataaatactt ctttgaaaga
      541 agaaaattct agaccagtgt ccttatctga tttggctcta ggtcttaagg acga
//
LOCUS       DATRQA010000300          589 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.304, whole genome shotgun sequence.
ACCESSION   DATRQA010000300 DATRQA010000000
VERSION     DATRQA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.304"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..570
                     /locus_tag="VBD27_002173"
     CDS             <1..570
                     /locus_tag="VBD27_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO7026117.1"
                     /translation="NIKLLHNSYDLELLANYIMDLEAKVKNGLIIDFVRSVSPILYRL
                     FMILLAQEVPHLHDYIYNARDDHYDTWKFKELKESNHPVLLAFSERWHDSRLTSKSLA
                     ECLQLTDLDEEVKSTIIQLRQFEKSVRNPLAHLIKPFDEQELYRTTQFSSQAFLDQII
                     FLAKVIGVEYDTVNFHYDTVNKLIIKILE"
BASE COUNT          198 a           97 c          101 g          193 t
ORIGIN      
        1 aatataaagc ttttgcataa ctcgtatgat ttagagttgc tggcaaatta catcatggat
       61 ttagaagcaa aagtaaaaaa tggattaatc atagattttg tccgtagtgt tagtcctatt
      121 ttatatcgtc tatttatgat tttacttgca caagaagtac ctcacttgca tgattacatc
      181 tataatgcaa gagatgatca ctacgatact tggaagttta aagaattaaa ggagtcaaac
      241 catccagtcc ttttggcatt ctctgaaagg tggcacgata gtcgcttgac ttctaaaagc
      301 cttgcagaat gtttacaatt aaccgacctt gatgaagaag tgaaatcgac catcattcaa
      361 ttaagacagt tcgaaaaatc agtcagaaat cctttggctc acctgattaa accttttgat
      421 gagcaagaac tatatcgtac aactcaattt tcttctcaag catttttaga ccagattatc
      481 ttcttggcaa aggtaattgg tgttgagtat gatactgtta attttcacta cgatacggtt
      541 aacaagctta ttataaagat acttgagtaa tttaaaaaca gttctgaga
//
LOCUS       DATRQA010000301          585 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.305, whole genome shotgun sequence.
ACCESSION   DATRQA010000301 DATRQA010000000
VERSION     DATRQA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.305"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..299
                     /locus_tag="VBD27_002174"
     CDS             <1..299
                     /locus_tag="VBD27_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026118.1"
                     /translation="LIIFIVFSGIYYFYYLPKNQGKKWKFNQWKLQKGKKEQLKINYG
                     IIFGLVMLSPVFLVGSIENIFGLIGGVLFTATFTALIVDSLYAAYYIHKYPEYE"
BASE COUNT          198 a           62 c          110 g          215 t
ORIGIN      
        1 tgttaattat ttttattgta ttttctggaa tatattattt ctattattta ccaaaaaatc
       61 aagggaaaaa atggaaattt aatcagtgga aattacaaaa aggaaaaaag gaacaattaa
      121 aaattaatta tggaattatt tttggtttag tgatgctttc tccagtcttt cttgtaggta
      181 gtattgaaaa tatttttggt ttgattggag gtgtattatt tacggcaact tttacagctc
      241 tgattgtcga ttccctttat gcagcatatt atattcataa atatcccgaa tatgagtgat
      301 aaattaatta atttaaggta ggagcaataa atatctagag aaaatgaagt gggtagaata
      361 gtctctacgc ttgctgagag tgtttttgta gtaatcataa caactccaat ttgtaccagt
      421 agtagcagtt gttataggta ctttggccat tgtggagtaa aagcactcta tgaaaatgtt
      481 aaagatgtaa gaaattttgt ggagccgttt catgagcaaa tattattgat caagattata
      541 ttttgattat ctggcaggat ttttccagag aggtcaattt agaac
//
LOCUS       DATRQA010000302          580 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.306, whole genome shotgun sequence.
ACCESSION   DATRQA010000302 DATRQA010000000
VERSION     DATRQA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.306"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            23..>580
                     /locus_tag="VBD27_002175"
     CDS             23..>580
                     /locus_tag="VBD27_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO7026119.1"
                     /translation="MSNHSRRHQKKHSHTPLRVINLFLLVIFILLSVVSLFLMYRHHF
                     LAFRHLNVIYGVVIILIILASLFLCIKNKARIFTTIILVLSSIFVATTLYGFKSTIDL
                     TNNLNKTASYSEIEMSVVVPKDSKITNIEAVSKLAAPVKNDTSNITDLIEHIKSEKGI
                     SITPQKTDSYQDAYNRIKNGDSQAMV"
BASE COUNT          202 a           89 c           81 g          208 t
ORIGIN      
        1 ttttaagtat tggagaatta caatgtctaa tcattcgcgc cgtcaccaaa agaaacactc
       61 acatacacct ctacgggtga ttaatttatt tcttttggtg atttttattt tgttaagtgt
      121 agtctcatta tttcttatgt atcgtcacca ttttttggca tttagacact tgaacgtcat
      181 ttatggagtt gtaattattt taatcatttt agcaagttta tttctttgta ttaagaataa
      241 agctagaatt tttacaacta taattttagt actatcctct attttcgttg ctactacttt
      301 atatggattt aagtcaacca ttgatttgac aaataatcta aataaaactg cttcatactc
      361 tgaaattgag atgagtgtag ttgtaccaaa agattctaaa ataaccaata tagaagctgt
      421 cagcaaatta gccgcaccag ttaaaaacga tacttcaaat attactgatt tgatagaaca
      481 tataaaatca gaaaaaggaa tctctattac accacaaaaa acagattctt accaggatgc
      541 atacaataga attaaaaatg gtgatagtca agctatggtt
//
LOCUS       DATRQA010000303          573 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.307, whole genome shotgun sequence.
ACCESSION   DATRQA010000303 DATRQA010000000
VERSION     DATRQA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.307"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            7..567
                     /locus_tag="VBD27_002176"
     CDS             7..567
                     /locus_tag="VBD27_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001905871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEO7026120.1"
                     /translation="MSYEKFVYYMLHKPKGVISATDDPSHKTVLDLLDKTARDKAVFP
                     VGRLDIDTTGLLLITNNGELAHKMLSPKKHVDKCYEAKISGIMTEDDILAFDKGIILK
                     DFTCLPALLEIVEVNQVKKQSLVKITIKEGKFHQVKRMVAACGKEVLELKRLSMGNLQ
                     LDKQLESGQWRRLTIKEIEKLEKHMQ"
BASE COUNT          219 a           76 c          117 g          161 t
ORIGIN      
        1 actctagtgt catatgaaaa gtttgtttat tatatgcttc ataaacccaa aggtgttata
       61 tcagcaacgg atgatccttc gcataaaacc gtattagatt tgctagacaa aactgctcga
      121 gataaagctg tttttccagt ggggcgttta gatattgata caacaggcct tttgttaata
      181 actaataatg gggaattagc gcataaaatg ttatcaccta aaaaacacgt tgataaatgc
      241 tatgaggcaa aaatctcagg gataatgaca gaagacgata ttttggcatt tgataaaggg
      301 attattctga aagattttac ttgtttacca gcattattag aaattgtgga agtaaatcag
      361 gtgaagaagc agagtcttgt taaaatcact attaaggaag gaaaattcca tcaagttaaa
      421 cgtatggttg ctgcgtgtgg caaggaagtc ttagagctaa agcgtctaag tatgggaaat
      481 ctacaattag ataaacagct agaaagtgga caatggagac ggctaacaat aaaagaaata
      541 gaaaaattag aaaaacatat gcaataatat aaa
//
LOCUS       DATRQA010000304          573 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.308, whole genome shotgun sequence.
ACCESSION   DATRQA010000304 DATRQA010000000
VERSION     DATRQA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.308"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(260..490)
                     /locus_tag="VBD27_002177"
     CDS             complement(260..490)
                     /locus_tag="VBD27_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026121.1"
                     /translation="MKLKISVLTAASAVTLLLATSASLAKADTVSYPSRDAGFIYSRG
                     YAPSESSYPSRDAGFIYSRGYAPKHFLSAYDK"
BASE COUNT          206 a           79 c          112 g          176 t
ORIGIN      
        1 attaaatatc aatcaaaagt aataagttca aagatatatt aaacctttat taatgaggtt
       61 tagatttgac aagtgatgtt aagtgaaaga tgaatgagga aaatacccaa cgagaaagta
      121 gctaatggct atcattgtgc atataaaaaa acgatcgatg agtgatcgtt tttcagcaaa
      181 ctaaataatt atttttaatg taattgaatg aaaatttata cttgtgaaag tgtatcggtt
      241 aagttaataa ggtcagtttt tatttatcgt aagcacttag gaagtgtttt ggagcgtatc
      301 cacgagagta gataaacccg gcatcgcgtg aaggatagct tgattcagaa ggcgcatacc
      361 cacgagagta gataaatccg gcatcacgtg aaggataaga cactgtgtct gccttcgcaa
      421 gacttgctga agtagctaat aatagagtga cagctgaagc tgctgttaaa acactgattt
      481 taagtttcat tataaaatcc tttcaaagat aataaactat tcttgcacag tggttagttc
      541 attttgtgca tactatatta tatcatcaaa agc
//
LOCUS       DATRQA010000305          557 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.309, whole genome shotgun sequence.
ACCESSION   DATRQA010000305 DATRQA010000000
VERSION     DATRQA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.309"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..484)
                     /locus_tag="VBD27_002178"
     CDS             complement(<1..484)
                     /locus_tag="VBD27_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO7026122.1"
                     /translation="MIDFLLLKSLHSLLGLTITVCDQNFSVIREYKSEKTISLFYNHY
                     LILSNFSKTQHDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNVIDPLLLKKKLTETQI
                     NASEQDYFIDRLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFD
                     L"
BASE COUNT          201 a           74 c           98 g          184 t
ORIGIN      
        1 ctagatcaaa ggagttgctc caccctttcg tgtagtaatg taagggctct gataatttat
       61 ctttcacaac cccagttaga caataattgg ttactattag taattctcgg atttgactaa
      121 gtgagaagaa aggtaattgg gatagtctat caataaagta gtcttgttca ctagcattga
      181 tttgtgtttc agtcagcttt ttcttaagaa gtaaagggtc aataacattg ctacgccagg
      241 gaccgatgat aatataatat tgttgaatat ggtggacaag aaaaagttct cctaaagagc
      301 cataatgaaa taaaaaatca tgttgagttt tactaaagtt acttaaaata aggtaatgat
      361 tgtaaaaaag tgaaatagtt ttctcggatt tatactctct gataacagaa aaattttgat
      421 cacaaacagt aatagttaga cctagaagac tgtgtaatga tttcaacagt agaaaatcaa
      481 tcatatgagt taccaccttt gatttaatat gttatctatt ttagagaata tttctttaaa
      541 gtcaacagaa taattag
//
LOCUS       DATRQA010000306          543 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.310, whole genome shotgun sequence.
ACCESSION   DATRQA010000306 DATRQA010000000
VERSION     DATRQA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.310"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..>543)
                     /locus_tag="VBD27_002179"
     CDS             complement(<1..>543)
                     /locus_tag="VBD27_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEO7026123.1"
                     /translation="SFANYFVINEFFRNVDAGKFSTYLYKDLRDRAKLVVWDFNNAFD
                     NQIEGRVDEADFTLTDAPWFNMLIKDKAFIDLVVHRYKELRKGVLATEYLSNYIDETR
                     HFLDPAIDRNYKKWGYVFDLKNTDPRNYLIPTERNVTSYHKSVEQLKDFIKKRGRWMD
                     RNIETLYQYSAPSKNTNTLL"
BASE COUNT          173 a          104 c           74 g          192 t
ORIGIN      
        1 ccagtaaagt attagtattt ttcgatggtg cactatattg atacaaggtc tcaatatttc
       61 tatccatcca tctgccacgc tttttgataa aatcttttaa ttgttctact gatttgtgat
      121 aacttgtcac attgcgttca gtaggaatca aataattcct tggatcagta ttctttaaat
      181 caaagacata gccccatttt ttataatttc gatcaatagc tggatctaaa aaatgccttg
      241 tctcgtctat gtaattagat aaatattctg tcgctagaac tccctttcgt aattctttat
      301 agcgatgaac aaccaggtct ataaatgctt tgtccttaat gagcatattg aaccacggtg
      361 catccgtcaa ggtaaaatct gcctcatcaa cacgtccttc aatttgatta tcaaaagcat
      421 tattaaaatc ccaaactact agttttgctc tatcacgaag atctttatat agataggttg
      481 aaaatttacc cgcatccaca tttctaaaaa attcattgat gacaaaataa tttgcaaatg
      541 att
//
LOCUS       DATRQA010000307          534 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.311, whole genome shotgun sequence.
ACCESSION   DATRQA010000307 DATRQA010000000
VERSION     DATRQA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.311"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..532)
                     /locus_tag="VBD27_002180"
     CDS             complement(<1..532)
                     /locus_tag="VBD27_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HEO7026124.1"
                     /translation="MIEFFSNIRTEIPQMPLLIHSLILSVLPFLMWLTLVNRDKPLYK
                     TIWSILLGLQLITIYTWFFWAKLPLSESLPLYHCRIGMFVVLLARPRILKDYFALLGV
                     VGGVLAMIHPDFYPYQFLHVTNIFFFIGHFALFVLSLLHLMTQSNLDKLNPKLIIQLT
                     LLINMSLIFINLLTGGN"
BASE COUNT          212 a           89 c           91 g          142 t
ORIGIN      
        1 aatttccccc agtcaagagg ttaataaaaa tcaaagacat attaatgaga agcgttagtt
       61 gtataatcaa ttttggattt aatttgtcca aattggactg tgtcatcaga tgtagtaaac
      121 taaggacaaa taaagcaaaa tgtccaataa agaagaaaat attagtcaca tgtagaaact
      181 ggtaagggta aaagtctggg tgaatcattg ccaatactcc acctactact cctaacaatg
      241 cgaaataatc ctttaatatt ctaggcctcg ctaagagaac gacaaacatg cctattcgac
      301 aatggtaaag gggaagactt tcagataaag gcaattttgc ccagaaaaac caagtataaa
      361 tcgtaattaa ctgaagtcct aaaaggatac tccaaatagt tttatacaaa ggcttatctc
      421 tattaaccaa agtcagccac atcagaaaag gtaagacaga taaaatcaaa ctatggataa
      481 gtaaaggcat ctgcggaatc tctgttctga tattagaaaa gaattctatc attt
//
LOCUS       DATRQA010000308          525 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.312, whole genome shotgun sequence.
ACCESSION   DATRQA010000308 DATRQA010000000
VERSION     DATRQA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.312"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            126..>525
                     /locus_tag="VBD27_002181"
     CDS             126..>525
                     /locus_tag="VBD27_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026125.1"
                     /translation="MLNSDFIISKSLANYIHHRRLEVGVSSTDLAEISNMSKSDWESF
                     EKNGGAIPLKSKDIILDLLFLERFPKEKECDFIDKLFEEAKENKLWPEKIYQTMGLTP
                     ALSFIAGCEILSDDINNDLEELSKLPKESHL"
BASE COUNT          187 a           67 c           96 g          175 t
ORIGIN      
        1 tatgaatcat tacaacatct ctagaaaatc atgatgtgct aaatcaaaag atgcatgtgt
       61 ttttacttga aaaaaatttt ttatatgtga taatcataat gaataggaat caaaaggaga
      121 acggaatgct aaattctgat tttattatta gtaagtcact tgctaactac attcaccatc
      181 gtaggttaga agttggcgtg tcgtcaactg atttggcaga aatatccaat atgtctaaat
      241 cagattggga atcatttgaa aaaaatggtg gggcaattcc tttgaaatct aaggatatca
      301 tacttgactt attatttttg gagagattcc ctaaagagaa agaatgtgat tttatagata
      361 aattatttga ggaagcaaag gaaaataaac tttggcctga aaagatttac cagactatgg
      421 gacttactcc tgctctttca tttattgctg gttgtgaaat actttctgat gatattaata
      481 atgatttgga ggaattatct aaactaccta aagaaagtca tctgg
//
LOCUS       DATRQA010000309          521 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.313, whole genome shotgun sequence.
ACCESSION   DATRQA010000309 DATRQA010000000
VERSION     DATRQA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.313"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            1..519
                     /locus_tag="VBD27_002182"
                     /pseudo
     CDS             1..519
                     /locus_tag="VBD27_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445921.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT          204 a           67 c           74 g          176 t
ORIGIN      
        1 gttcagataa cactaattta aaatttttaa aagctttctc agaattgtta aagatgagga
       61 gctttgagca aattaaagtt agcgatttag caaaaaaagc tcgacttagt agaagatctt
      121 tctataatca ttacaattca aaagaagact tccttaggga gtctatttta ataatctttg
      181 atgatatcac aaaaatttta aataatgatt tattatatga agaagtggtc ttaaaggaga
      241 tgcttagcta tatgtatata aataaagaaa ttattaaatc ctttgttttt tccgaatatt
      301 gacaacataa ttaaaaacta tatcaaagag atgattacac attccgatat accagactta
      361 caaaaacaac tggaaacagc atatcaagtt ccatattctt tcgctcttgg tatttatatc
      421 tcaacaattg aaagtattat tttaaattgg atagatcatg actttactga agagcctgaa
      481 gaaatagcaa gatatattac atctgtcgtg agaatttaat a
//
LOCUS       DATRQA010000310          516 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.314, whole genome shotgun sequence.
ACCESSION   DATRQA010000310 DATRQA010000000
VERSION     DATRQA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.314"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..>516
                     /locus_tag="VBD27_002183"
     CDS             <1..>516
                     /locus_tag="VBD27_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSag9 family transposase"
                     /protein_id="HEO7026126.1"
                     /translation="EKLLVESREGIPLNKETFWKIDRVLSNGVKKGQRIYHILKTNDL
                     EVSSSTVYRHIKKGYLSITPIDLPRAVKFKKRRKSTLPPIPKAIKEGRRYEDFIEHMN
                     QSELNSWLEMDTVIGRIGGKVLLTFNVAFCNFIFAKLMDSKTAIETAKHIQVIKRTLY
                     DNKRDFFELFPV"
BASE COUNT          172 a          115 c          102 g          127 t
ORIGIN      
        1 gaaaaacttt tagttgaatc tagagaagga atccctttga acaaagagac cttctggaaa
       61 atagatagag tgctttctaa tggggtcaag aagggccaac gtatctatca tatcctcaaa
      121 accaacgatc tagaagtgag ttcttcaacc gtttatcgac acatcaagaa aggctaccta
      181 tccatcacac caatcgacct acccagagct gtgaagttca aaaaaaggcg aaagagcaca
      241 ctccctccta ttccaaaagc gattaaagaa gggcgacggt acgaggattt tatagaacac
      301 atgaaccaat cagagctgaa ctcctggctt gaaatggaca cggttattgg acggattggt
      361 ggaaaggtcc ttctcacctt caatgtcgcc ttctgtaact tcatctttgc caaactgatg
      421 gattctaaga cagctatcga aactgctaaa cacatacagg tcattaagag gacactctat
      481 gataacaaga gagacttctt cgaactcttt cctgtt
//
LOCUS       DATRQA010000311          513 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.315, whole genome shotgun sequence.
ACCESSION   DATRQA010000311 DATRQA010000000
VERSION     DATRQA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.315"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..>513)
                     /locus_tag="VBD27_002184"
     CDS             complement(<1..>513)
                     /locus_tag="VBD27_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026127.1"
                     /translation="GHKINSVKYRTAIQNKKAVLPHYRPLSPKDIIELKDIDVKYSEL
                     PWKLYRNTGKLKHPRFRLIPKKDKNRDHERKIEWPTFYLGLSRLYPLGEVEENLLIKD
                     LNSGYEDKISKIHKDIMTSSDEYEAVQVTNINGMKTGVLVNTTTYPGTANSAGQDNLG
                     QILSAVFSFE"
BASE COUNT          145 a           83 c           71 g          214 t
ORIGIN      
        1 ttttcaaatg aaaaaacagc agatagaatt tggcccaaat tatcctgacc tgctgaatta
       61 gctgttccag gatatgtagt cgtgttgaca agtacacctg ttttcatccc atttatgttt
      121 gttacttgta cagcttcgta ttcatctgat gaagtcataa tatctttatg aatttttgaa
      181 attttatctt cataaccact gtttaaatct ttaattagta aattttcctc aacttctcca
      241 agtggatata acctagaaag tcctaagtaa aaagttggcc actctatttt tctttcatga
      301 tctctatttt tatctttttt agggataagt ctaaaccttg gatgttttag ttttccagta
      361 tttctgtata acttccatgg taattcacta tatttaacat ctatatcttt taattcaata
      421 atatcttttg gcgaaagggg tctataatgt ggtaaaactg cttttttatt ttgtattgca
      481 gttctatatt ttacactgtt gattttatga cca
//
LOCUS       DATRQA010000312          510 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.316, whole genome shotgun sequence.
ACCESSION   DATRQA010000312 DATRQA010000000
VERSION     DATRQA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.316"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
BASE COUNT          163 a          109 c           68 g          170 t
ORIGIN      
        1 ggtaacacca aaacatacac gaacttttat tcatatgtgt taccatttta catctttcat
       61 attgagatat ttgattagca atacctcctt gactaacaaa aattactaac acatctaggg
      121 gtatacccta gtttatattt gttcatgatt acctcaagcg agcatgcgct aatggtaatg
      181 aactattacc acattgtctg tcttccttaa attatataag gacttatgtg tcaccgttac
      241 tgtagcgacc tacatctaaa cctaacataa gtgcccctcc aactatttct aggaaatgaa
      301 gaaaacttca aatccttgtg actgtttgca acatcaatgc attgataaca attttagtgt
      361 atcgccattc ttatagcgac ccatattaaa aattaacata agcgctacct ccattaacta
      421 aagttagctt gcctaccaat ctttcttttg gagactagtc tccattctta tacctagaga
      481 tattgtaata gatcatggat aacctccttg
//
LOCUS       DATRQA010000313          491 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.317, whole genome shotgun sequence.
ACCESSION   DATRQA010000313 DATRQA010000000
VERSION     DATRQA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.317"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..>491
                     /locus_tag="VBD27_002185"
     CDS             <1..>491
                     /locus_tag="VBD27_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO7026128.1"
                     /translation="SYKYQYHFENNILKAVKSGSEFLLKETVEQFSNSIVPIISGDEL
                     RSEKNYSIMIYDRLSQATIQAGLDIETAYRARDRFIKENESTISLNEVLKLRDTAILF
                     YTQQVHSLKKHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQE
                     KHIT"
BASE COUNT          190 a           86 c           70 g          145 t
ORIGIN      
        1 tcttacaaat atcagtatca ttttgaaaat aatattctaa aagctgttaa atcaggtagt
       61 gaatttcttt tgaaagaaac agtggaacaa tttagcaatt ctatcgtacc tataatcagt
      121 ggagatgaat tgcgatctga aaaaaactac tccatcatga tttatgatcg tctctcacag
      181 gctaccatcc aagcaggact tgatatcgaa acagcttatc gagcacgaga tcgttttata
      241 aaagaaaacg agtcaactat aagtctaaat gaagttttaa aattacgtga tactgctatc
      301 ttattctata ctcaacaagt tcattcttta aaaaaacatc tcgaaacccc tcattctcaa
      361 actattgtcg cagtgattcg atatcttgaa aataatctaa atcgctttat taaaacagaa
      421 gaaatagcta aagaatgtca catgagtgaa tcgaagttac gaaaactatt taagcaagaa
      481 aaacacatca c
//
LOCUS       DATRQA010000314          481 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.318, whole genome shotgun sequence.
ACCESSION   DATRQA010000314 DATRQA010000000
VERSION     DATRQA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.318"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..>481
                     /locus_tag="VBD27_002186"
     CDS             <1..>481
                     /locus_tag="VBD27_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7026129.1"
                     /translation="QQTHPLIEVILIDDGSTDNSGEICDNLSQEDNRILVFHKKNGGV
                     SSARNLGLDKSTGEFITFVDSDDFVAPNMIEIMLKNLITENADIAEVDFDISNERDYR
                     KKKRRNFYKVFKNNNSLKEFLSGNRVENIVCTKLYKKSIIGNLRFDENLKIGEDLLFN
                     "
BASE COUNT          188 a           45 c           93 g          155 t
ORIGIN      
        1 caacagactc atccattgat agaagttata ctaattgatg atggatccac tgataatagt
       61 ggagaaattt gtgataattt atctcaggaa gataatcgca tacttgtatt tcataaaaaa
      121 aatggagggg tctcttcggc aaggaaccta ggtctagata aatccacagg agaattcata
      181 acatttgtgg atagtgatga ttttgtagca ccgaatatga ttgaaataat gttaaaaaat
      241 ttaatcactg agaatgctga tatagcagaa gtagattttg atatttcgaa tgagagagat
      301 tatagaaaga agaaaagacg aaacttttat aaagttttta agaataataa ctctttgaaa
      361 gaatttttat caggtaatag agtggaaaat attgtttgta caaaattata taaaaaaagt
      421 ataattggta acttgaggtt tgatgagaac ttaaaaattg gtgaggattt actttttaat
      481 t
//
LOCUS       DATRQA010000315          475 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.319, whole genome shotgun sequence.
ACCESSION   DATRQA010000315 DATRQA010000000
VERSION     DATRQA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.319"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            63..233
                     /locus_tag="VBD27_002187"
     CDS             63..233
                     /locus_tag="VBD27_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026130.1"
                     /translation="MNKILAIGTEIAPVIGAVASIGVKALYNNSKSFRNVISSTGNVI
                     NNIDKHFTKINS"
     gene            246..>475
                     /locus_tag="VBD27_002188"
     CDS             246..>475
                     /locus_tag="VBD27_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026131.1"
                     /translation="MGIKSYRRVELVAKLNFRYYLVSISGRYYIIDYANPSDIKDYFI
                     GIFPERVEKYQIYDVTADYKNFLVNKKSRLYL"
BASE COUNT          188 a           41 c           86 g          160 t
ORIGIN      
        1 aggtattgat cgaattaaaa aaaaatagta atagtggaac caatatataa aaggagttgt
       61 gattgaataa gatattagca attggtactg aaattgcacc tgtgatagga gcagttgctt
      121 cgattggtgt aaaagcattg tataataatt ctaaatcatt tagaaatgtt ataagtagta
      181 caggaaatgt aattaacaat attgataaac attttacaaa aataaatagt taggtacgtg
      241 aactaatggg aataaaaagt tatagacggg ttgagcttgt agcaaaactt aattttagat
      301 attatctagt tagtattagt ggacgttatt acattattga ttatgccaat cctagtgata
      361 taaaagatta ttttataggt atttttccag aaagagtaga aaaatatcaa atttatgatg
      421 tgactgcgga ttataagaat tttttagtaa acaaaaaaag tcgcctttat cttta
//
LOCUS       DATRQA010000316          450 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.320, whole genome shotgun sequence.
ACCESSION   DATRQA010000316 DATRQA010000000
VERSION     DATRQA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.320"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..450)
                     /locus_tag="VBD27_002189"
     CDS             complement(<1..450)
                     /locus_tag="VBD27_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO7026132.1"
                     /translation="MCLIEDNKGRIVMQIRDPKRYSWSGAALPGGHIEEGESLYDALV
                     REVREETGLTISNPKLVGMKHWYTDEGIRYLVFLYKTYDFSGELQSSDEGDVIWIERS
                     QLDKIELAYDMLNLLRVFDEDDISEMFYSKRLEDGFIRKILLNKKELG"
BASE COUNT          139 a          101 c           60 g          150 t
ORIGIN      
        1 tcctagctct tttttgttta ataaaatttt tcttataaaa ccatcttcaa gtcgcttact
       61 ataaaacatc tctgagatgt catcttcgtc aaatactctc aaaaggttaa gcatatcata
      121 tgctagttca atcttatcaa gttgtgaacg ttcaatccat ataacatcac cttcatctga
      181 cgattgtaat tcacctgaaa aatcatatgt tttataaagg aataccaaat aacggatgcc
      241 ttcatctgtg taccaatgct tcataccaac taatttggga ttacttattg ttaaacctgt
      301 ctcctcgcga acttctcgaa cgagagcatc atacaagctt tctccttctt caatatggcc
      361 gcctggtaga gcagctcctg accaagaata acgtttagga tcacggattt gcatcacaat
      421 acgtccttta ttatcttcaa taagacacat
//
LOCUS       DATRQA010000317          446 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.322, whole genome shotgun sequence.
ACCESSION   DATRQA010000317 DATRQA010000000
VERSION     DATRQA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.322"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            233..>446
                     /locus_tag="VBD27_002190"
     CDS             233..>446
                     /locus_tag="VBD27_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEO7026133.1"
                     /translation="MESHLVRIINRLELMATDGGNLKRNFEREGVVVAEVSFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEI"
BASE COUNT          156 a           55 c           89 g          146 t
ORIGIN      
        1 tgcttttatt atagcaccta aagcatagaa atcaagaaaa aagttctgtt tttagcactg
       61 aatatattag agtgctaaat tgaaaaatag atgactactg agaaaaatcg ttatttgcgg
      121 attttagacg ataaaatcgt taaaaagtta gtaaaaatgt tgcttttcat gttgctttgg
      181 tcacttttgt gttaaaatag aaaagactag aaaatatata ggagtaagta agatggaatc
      241 acatttagtg agaatcatca atcgcctcga attaatggcg actgatggtg gtaatttaaa
      301 acgtaatttt gaacgtgaag gtgttgttgt agcagaagta tcttttagta atgaccctga
      361 aaacggtcct gttttcacat tacgtgatgt agaagcgcgt gaatcatatt catttgacag
      421 cattgacctt atcgcaatgg aaattt
//
LOCUS       DATRQA010000318          440 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.324, whole genome shotgun sequence.
ACCESSION   DATRQA010000318 DATRQA010000000
VERSION     DATRQA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.324"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..223)
                     /gene="ipk"
                     /locus_tag="VBD27_002191"
     CDS             complement(<1..223)
                     /gene="ipk"
                     /locus_tag="VBD27_002191"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEO7026134.1"
                     /translation="MKIFEKAPAKLNLGLDIKGRCDDGYHELAMIMVSIDLNDYVTIS
                     ELKEDCIVIDSDSSKMPLNNDNDVFKAADI"
     gene            complement(363..436)
                     /locus_tag="VBD27_002192"
     tRNA            complement(363..436)
                     /locus_tag="VBD27_002192"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(400..402),aa:Asn,seq:gtt)
BASE COUNT          163 a           92 c           54 g          131 t
ORIGIN      
        1 ttatatctgc agctttaaac acatcattat cattatttaa aggcattttg ctactatcag
       61 aatcgataac aatacaatct tcctttagct cagaaatggt aacgtagtca ttaagatcaa
      121 tactaaccat aatcatagct aattcatgat aaccatcgtc acatcgtcct ttaatatcta
      181 atcctaaatt aagtttggca ggagctttct caaaaatttt cataaaacct ccctaataaa
      241 atatagaata tccatattat aacataacaa atgacaaatt cggacaatta agatgctagc
      301 cgaccttaag gaataataat tccaataaaa aaaggctaac caaagttagt ctcctttatc
      361 tactccgcca gtaggactcg aacctacgac atcatgatta acagtcatgc gctactacca
      421 actgagctat ggcggattat
//
LOCUS       DATRQA010000319          425 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.326, whole genome shotgun sequence.
ACCESSION   DATRQA010000319 DATRQA010000000
VERSION     DATRQA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.326"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..380
                     /locus_tag="VBD27_002193"
     CDS             <1..380
                     /locus_tag="VBD27_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO7026135.1"
                     /translation="LTMRKWSIDLITRIERLNWVIRGWINYFSLGNMKSIMTQIDERL
                     RTRIRVIIWKQWKKKAKRLWGLLKLGVARWIADKVSGWGDHYQLVAQKSVLKRAISKP
                     ALAKRGLVSCLDYYLERHALKVS"
BASE COUNT          129 a           79 c          116 g          101 t
ORIGIN      
        1 aactgacgat gaggaaatgg agcattgacc tgataactcg cattgaacga ttgaactggg
       61 tcattcgagg atggataaac tatttctcgc ttggcaatat gaagagtatc atgacacaaa
      121 tagatgagcg tctgcgaacc cgtattcgag tgattatctg gaagcaatgg aagaagaaag
      181 caaagcgcct atggggactc ttaaaactag gagttgctag atggatagcc gataaagttt
      241 ctggatgggg tgaccactat cagttggtag ctcagaagtc ggtactcaaa cgtgctatat
      301 caaaaccagc cctcgcaaag cgaggactgg tcagttgctt agattactat cttgaacgac
      361 atgcgttaaa agttagttga accgccgtat gccgaacggc acgtacggtg gtgtgagagg
      421 ggcta
//
LOCUS       DATRQA010000320          409 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.328, whole genome shotgun sequence.
ACCESSION   DATRQA010000320 DATRQA010000000
VERSION     DATRQA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.328"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(17..394)
                     /gene="mscL"
                     /locus_tag="VBD27_002194"
     CDS             complement(17..394)
                     /gene="mscL"
                     /locus_tag="VBD27_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO7026136.1"
                     /translation="MIKELKEFLFKGNVLDLAVAVILGAAFNAIITSLVKDVITPLIL
                     NPVLKAAGVSNIAQLSWNGVAYGNFLSAVINFLIVGTTLFFIVKAANKVMAKKPAEEE
                     IIEVVEPTQEQLLAEIRDLLANK"
BASE COUNT          142 a           73 c           69 g          125 t
ORIGIN      
        1 taatccgagt cttttattat ttgttagcga gtaagtcgcg gatttcagca agtaattgct
       61 cttgtgttgg ttcaacaact tcaataattt cttcttcagc aggtttctta gccataacct
      121 tgttagcagc tttaacgatg aagaataaag tagtaccaac gattaagaaa ttaataactg
      181 cacttaagaa attaccgtaa gcaacaccat tccatgaaag ctgtgcgata tttgaaacgc
      241 cagcagcttt taatacagga ttaaggataa gcggtgtaat aacgtcttta acaagtgaag
      301 tgatgatagc attaaaagct gcacctaaaa taacagcaac agcaaggtct aaaacattac
      361 ccttaaataa aaattctttt agttctttaa tcatgtgaac aagctcctt
//
LOCUS       DATRQA010000321          299 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.333, whole genome shotgun sequence.
ACCESSION   DATRQA010000321 DATRQA010000000
VERSION     DATRQA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.333"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..244)
                     /locus_tag="VBD27_002195"
     CDS             complement(<1..244)
                     /locus_tag="VBD27_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7026137.1"
                     /translation="MKNNIIFSIEQYINDQEMPKGKQKVILSAIELFASQGFHGTSTA
                     QLAKNAEVSQATIYKYFETKDKLLVFILELIVQTIGR"
BASE COUNT           89 a           45 c           52 g          113 t
ORIGIN      
        1 ggcgtccaat agtttgtacg attaactcga gaataaagac caaaagcttg tctttagttt
       61 caaaatattt atatatagtt gcttggctaa cttctgcatt tttagctagt tgagcagtgg
      121 aagttccatg gaagccttgt gaggcaaaga gctctatagc ggataaaata actttttgtt
      181 ttccttttgg catctcttgg tcattaatat actgttcaat agaaaaaata atattatttt
      241 tcatacttag ttccttttca aatggtatta tactttacgg tatcgtttta gtccaaaga
//
LOCUS       DATRQA010000322          274 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.336, whole genome shotgun sequence.
ACCESSION   DATRQA010000322 DATRQA010000000
VERSION     DATRQA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.336"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
BASE COUNT          100 a           47 c           38 g           89 t
ORIGIN      
        1 gtagcgatga taaacaatga actgatgtct ggttaaggaa atagttatta agcaatacag
       61 attttatgat gcatactggc tgtttcaata atcatcccgt acttgacagt acctatctaa
      121 ttttacataa gttcacctcc caatttagct tacaataaat agacatttaa ctcttaaagc
      181 gaagaattac gtttactagt aaaattatca atctataagt tactagacac cagtgacaaa
      241 atagtgttgc ctaaatacta gattacatac cttt
//
LOCUS       DATRQA010000323          271 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.339, whole genome shotgun sequence.
ACCESSION   DATRQA010000323 DATRQA010000000
VERSION     DATRQA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.339"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..>271
                     /locus_tag="VBD27_002196"
     CDS             <1..>271
                     /locus_tag="VBD27_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor regulator N-terminal
                     domain-containing protein"
                     /protein_id="HEO7026138.1"
                     /translation="SDPLEQLAKKRKRKTLFKTVVLALITVLLVVGIAFKGFTYLTSK
                     NGQKTYHNFELLSEIAYPNISYDSLYYQPSGQFTGKVHADRFKDID"
BASE COUNT          106 a           40 c           43 g           82 t
ORIGIN      
        1 agtgatccac tggaacaatt agccaaaaaa aggaaaagga aaacattatt caagacagta
       61 gtattggcat taataactgt actattagta gttggaatag cttttaaagg atttacatat
      121 ttaacttcaa aaaatggtca aaaaacatat cataactttg aactactttc tgaaatagca
      181 tatccaaata tttcctatga tagtctctat taccagccaa gcggtcagtt tactggtaaa
      241 gtacatgctg atagatttaa agatatcgat g
//
LOCUS       DATRQA010000324          270 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.340, whole genome shotgun sequence.
ACCESSION   DATRQA010000324 DATRQA010000000
VERSION     DATRQA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.340"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..>270)
                     /locus_tag="VBD27_002197"
     CDS             complement(<1..>270)
                     /locus_tag="VBD27_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7026139.1"
                     /translation="KEMAGIDNWEHYQKEKKITIGFDNTFVPMGFESRSGDYTGFDID
                     LANAVFKEYGISVKWQPINWDMKETELNNGNIDLIWNGYSKTAERA"
BASE COUNT           89 a           59 c           30 g           92 t
ORIGIN      
        1 gcacgttctg ccgtttttga ataaccattc caaataaggt ctatattacc attattaagt
       61 tcagtttctt tcatatccca gttaataggc tgccatttca ctgaaatacc gtattcttta
      121 aaaacagcat tagctaaatc aatatcaaag ccggtatagt caccagaacg actttcaaat
      181 cccataggaa caaaagtatt atcaaatcca atagtaattt tcttttcctt ttgataatgt
      241 tcccaattat caataccagc catttcctta
//
LOCUS       DATRQA010000325          267 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.341, whole genome shotgun sequence.
ACCESSION   DATRQA010000325 DATRQA010000000
VERSION     DATRQA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.341"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..>267)
                     /locus_tag="VBD27_002198"
     CDS             complement(<1..>267)
                     /locus_tag="VBD27_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7026140.1"
                     /translation="YSYPNIDLEYLIQNHFSQWLGDGPHQLIPIMLWSILAIFLTLSG
                     LFVPDILFRPYSYFSVVSENLNAALSQHTDKIPYLYTFYTVKNS"
BASE COUNT          108 a           33 c           50 g           76 t
ORIGIN      
        1 agctattttt aacagtataa aaggtataca gataagggat tttatctgta tgttggctca
       61 aggcagcatt taaattttcg ctaactactg aaaaatagga gtaaggacga aataaaatat
      121 ctggtacaaa aagacctgat aaggttagaa aaatagctaa gatagaccaa agcataatag
      181 gaattaattg atgtggccca tcacccagcc attgagaaaa gtgattttgt ataaggtatt
      241 ctaaatcaat attaggataa ctatata
//
LOCUS       DATRQA010000326          266 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.342, whole genome shotgun sequence.
ACCESSION   DATRQA010000326 DATRQA010000000
VERSION     DATRQA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.342"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            complement(<1..>266)
                     /locus_tag="VBD27_002199"
     CDS             complement(<1..>266)
                     /locus_tag="VBD27_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEO7026141.1"
                     /translation="NPSQAQDVTQDVFLQILESDFSLPLDKIKAWMYRTTIRRYIDLY
                     RRNKRYNEILQKEFFIENNLYYTESEKFFELHEAILHLKKDYQT"
BASE COUNT           83 a           39 c           43 g          101 t
ORIGIN      
        1 aagtttgata atccttcttc aagtgtaata ttgcttcatg taattcaaaa aacttctctg
       61 attcggtgta atacaagtta ttttctataa agaattcttt ttgcaggatt tcgttatagc
      121 gtttatttct tcgataaaga tcaatatacc tcctaattgt agtacgatac atccaagctt
      181 ttattttatc aagtggaagg gagaaatcag attctagtat ttgtaaaaag acgtcttgag
      241 taacatcctg tgcttgacta ggatta
//
LOCUS       DATRQA010000327          265 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.denovo.343, whole genome shotgun sequence.
ACCESSION   DATRQA010000327 DATRQA010000000
VERSION     DATRQA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.denovo.343"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            <1..>265
                     /locus_tag="VBD27_002200"
     CDS             <1..>265
                     /locus_tag="VBD27_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7026142.1"
                     /translation="VTINYLTKHTGQINVVENPKNDYLRRDLMLKLGQPIKQSISVLT
                     EDDTARWYLAHLLNYYVQKRPNTKLQIDTLKMVDCHIGWSSIVS"
BASE COUNT           97 a           37 c           45 g           86 t
ORIGIN      
        1 gttactatta attatttaac aaaacatact ggtcaaatta atgttgtaga aaatcctaaa
       61 aatgattatt taagacgtga tttaatgtta aaactcggtc agccgattaa gcaatccatt
      121 tcagttttaa cggaagatga tacggcaagg tggtatctag ctcatttact taattattac
      181 gttcagaagc gaccaaatac aaaacttcaa attgacacat taaaaatggt tgattgtcat
      241 attggatgga gcagtattgt aagtc
//
LOCUS       DATRQA010000328         2573 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699685-rid18550523.guided.191, whole genome shotgun sequence.
ACCESSION   DATRQA010000328 DATRQA010000000
VERSION     DATRQA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699685
            Sequence Read Archive: SRR7789091
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789975.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789975.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,161
            Genes (coding)                    :: 2,097
            CDSs (with protein)               :: 2,097
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 43 of 64
            Pseudo Genes (incomplete)         :: 10 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 6 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699685-rid18550523.guided.191"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2012-12-12"
                     /collected_by="RMPRU"
     gene            155..271
                     /locus_tag="VBD27_002201"
     CDS             155..271
                     /locus_tag="VBD27_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO7026143.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            287..2206
                     /gene="tet(M)"
                     /locus_tag="VBD27_002202"
     CDS             287..2206
                     /gene="tet(M)"
                     /locus_tag="VBD27_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO7026144.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2325..2492
                     /locus_tag="VBD27_002203"
     CDS             2325..2492
                     /locus_tag="VBD27_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO7026145.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
BASE COUNT          880 a          383 c          522 g          788 t
ORIGIN      
        1 gctattattt aagtatctat ttaaaaggag ttaataaata tgcggcaagg tattcttaaa
       61 taaactgtca atttgatagt gggaacaaat aattggatgt ccttttttag gagggcttag
      121 ttttttgtac ccagtttaag aataccttta tcatgtgatt ctaaagtatc cggagaatat
      181 ctgtatgctt tgtatgccta tggttatgca taaaaatccc agtgataaga gtatttatca
      241 ctgggatttt tatgcccttt tgggcttttg aatggaggaa aatcacatga aaattattaa
      301 tattggagtt ttagctcatg ttgatgcggg aaaaactacc ttaacagaaa gcttattata
      361 taacagtgga gcgattacag aattaggaag cgtggacaaa ggtacaacga ggacggataa
      421 tacgctttta gaacgtcaga gaggaattac aattcagaca ggaataacct cttttcagtg
      481 ggaaaatacg aaggtgaaca tcatagacac gccaggacat atggatttct tagcagaagt
      541 atatcgttca ttatcagttt tagatggggc aattctactg atttctgcaa aagatggcgt
      601 acaagcacaa actcgtatat tatttcatgc acttaggaaa atggggattc ccacaatctt
      661 ttttatcaat aagattgacc aaaatggaat tgatttatca acggtttatc aggatattaa
      721 agagaaactt tctgccgaaa ttgtaatcaa acagaaggta gaactgtatc ctaatatgtg
      781 tgtgacgaac tttaccgaat ctgaacaatg ggatacggta atagagggaa acgatgacct
      841 tttagagaaa tatacgtctg ggaaattatt ggaagcatta gaactcgaac aagaggaaag
      901 cataagattt cataattgtt ccctgttccc tgtttatcac ggaagtgcaa aaaacaatat
      961 agggattgat aaccttatag aagtgattac gaataaattt tattcatcaa cacatcgagg
     1021 tcagtctgaa ctttgcggaa aagttttcaa aattgagtat tcggaaaaaa gacagcgtct
     1081 tgcatatata cgtctttata gtggcgtact gcatttgcga gattcggtta gaatatcgga
     1141 aaaggaaaaa ataaaaatta cagaaatgta tacttcaata aatggtgaat tatgtaaaat
     1201 cgataaggct tattccgggg aaattgttat tttgcaaaat gagtttttga agttaaatag
     1261 tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc cgcaccctct
     1321 actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc ttgatgccct
     1381 tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta cgacacatga
     1441 aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac tgttgcaaga
     1501 aaagtatcat gtggagatag aaataaaaga gcctacagtc atttatatgg aaagaccgtt
     1561 aaaaaaagca gagtatacca ttcacatcga agttccaccg aatcctttct gggcttccat
     1621 tggtctatct gtagcacagc ttccattagg gagcggagta cagtatgaga gctcggtttc
     1681 tcttggatac ttaaatcaat cgtttcaaaa tgcagttatg gaggggatac gctatggctg
     1741 tgaacaagga ttgtatggtt ggaatgtgac ggactgtaaa atctgtttta agtatggctt
     1801 atactatagc cctgttagta ccccagcaga ttttcggatg cttgctccta ttgtattgga
     1861 acaagtctta aaaaaagctg gaacagaatt gttagagcca tatcttagtt ttaaaattta
     1921 tgcgccacag gaatatcttt cacgagcata caacgatgct cctaaatatt gtgcgaacat
     1981 cgtagacact caattgaaaa ataatgaggt cattcttagt ggagaaatcc ctgctcggtg
     2041 tattcaagaa tatcgtagtg atttaacttt ctttacaaat ggacgtagtg tttgtttaac
     2101 agagttaaaa gggtaccatg ttactaccgg tgaacctgtt tgccagcccc gtcgtccaaa
     2161 tagtcggata gataaagtac gatatatgtt caataaaata acttagtgta ttttatgttg
     2221 ttatataaat atggtttctt gttaaataag atgaaatatt ttttaataaa gatttgaatt
     2281 aaagtgtaaa ggaggagata gttattataa actacaagtg gatattgtgt cctgtatgtg
     2341 gaaataaaac acgattaaag ataagggaag atactgaatt aaaaaaattc cccctctatt
     2401 gtccgaaatg cagacaagaa aatttaattg aaataaagca gttcaaagta actgtgatta
     2461 cagagccaga cgcaaagacg cagagccgat aaaatgagat taatacaatc tcattttatc
     2521 ggctctttcc gttatgtatg gattctttta attagtcttc gatgtttctt gct
//
